LOCUS       DAOSIE010000001        30072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_1743_length_30072_cov_4.626478, whole genome shotgun sequence.
ACCESSION   DAOSIE010000001 DAOSIE010000000
VERSION     DAOSIE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30072
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1743_length_30072_cov_4.626478"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /gene="lepA"
                     /locus_tag="PLI69_00005"
     CDS             complement(<1..1524)
                     /gene="lepA"
                     /locus_tag="PLI69_00005"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOG40905.1"
                     /translation="MKHIRNFCIIAHIDHGKSTLADRLLEFTNTIDRRDMATQVLDSM
                     DLEKEKGITIKSHAIQMDYVHKGEKYTLILIDTPGHVDFSYEVSRAIASCEGALLVVD
                     ATQGIQAQTISNLYLAIEHDLEIIPILNKIDVEAAMVDMVREQVTDLLGCKDEEVLLA
                     SARMGLGIEAILEAIVEKIPAPQGDPEAPLQALIFDSVFNSFRGIIAYFKVANGIIRK
                     GDKVKFVNTGKEYVADEVGVLKMKYLPRNEIECGSVGYIISGIKAAAEVKVGDTITHV
                     HNPCKSAIAGFEDVKPMLYAGMFPVSADEYEDLRASLEKFQLNDASLVFEPESSLALG
                     FGFRCGFLGMLHMEIVQERLFREFDMDVITTVPNVSYKVYTTQGECIEVHNPSGLPPT
                     TLIHHIEEPYIQAQIISKTEYYGPIMKLCLEKRGTLKNQHYISSDRIELTYDMPLSEI
                     VFDFYDKLKSISKGYASFDYHLTGFREAKLVKLDILLNGEPADALSTLIHQDHAYDFG
                     "
     gene            1588..2433
                     /locus_tag="PLI69_00010"
     CDS             1588..2433
                     /locus_tag="PLI69_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOG40906.1"
                     /translation="MNDIHTYQKIASQVRRDILRMVTGAGSGHPGGSMSSADILTALY
                     FKEMNVTPEGWNRAGNGHDIFFLSIGHISPIFYSVLARRGFFPISELGTFRAYGSRLQ
                     GHPSIDAALPGVHQASGSLGQGLSVAIGAALAKRLAGDNSRVFVLIGDGESQEGQIWE
                     AAAAAVHHKVDNLIAMTDWNKQQIDGSTEEVQSLGDLTLKWNAFGWECIVADGHDFNS
                     IFEAFDRANSLLGGGRPVMILFETEMGHGVDFMAGTHEWHGKSPSQEQCERGLAQLEE
                     TFGDY"
     gene            2445..3398
                     /locus_tag="PLI69_00015"
     CDS             2445..3398
                     /locus_tag="PLI69_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOG40907.1"
                     /translation="MKKYINTGNKDTRSGFGAGLSHLGTINPKVVALCADLTGSLKMD
                     AFAKAHPERFFQCGIAEANMIGMAAGLAIGGAIPFAGTFANFATGRVYDQIRQSVCYS
                     ETNVKIAASHAGITLGEDGATHQILEDMGLMRMLPNMVVINPCDYNQTKAATIAAAAW
                     QGPVYLRFGRPGVPNFTPEDQVFEIGKALHLNEGSDVTVIATGHLVWEAIQAAEILEK
                     EGISVDLLNIHTIKPLDHEAIISSAVKSGRVVCAEEHLIAGGLGEAVAGLLAREHPVP
                     MRFVAIDDRFGQSGKPAQLMSAYGLDTAHIVAAVKEIIGVK"
     gene            3428..3970
                     /locus_tag="PLI69_00020"
     CDS             3428..3970
                     /locus_tag="PLI69_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOG40908.1"
                     /translation="MEDKDIIGLYKSGQAERAFTFIVRKYSERLYWHIRQLEADHDDA
                     DDILQETFIKVWNALPAFREDSKLYTWLYRIATNESLTFLKRRKLRATFTLRDPDMDM
                     ASFIESDPLFDGDELQLSLQKAIATLPPKQKSVFVMRYFHEIKYEDISEIMGISVGAL
                     KASYHHAFNKVQEYLKKEGY"
     gene            4003..4476
                     /locus_tag="PLI69_00025"
     CDS             4003..4476
                     /locus_tag="PLI69_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40909.1"
                     /translation="MNEQYNITDDPHLKSNPFKAPEGYLESLEERILSLAGIVQGEAA
                     EEVVTPSGVWRRFGILKPALLLACTFGVIFGMGYGVLRLTGTLDKSPNGEDSYYAKMV
                     EKGLIKTKFIDWIEEDLEYDDFYEEDQDFDEEEVVSWLATQYSSMELAQLYYDEK"
     gene            4484..5005
                     /locus_tag="PLI69_00030"
     CDS             4484..5005
                     /locus_tag="PLI69_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40910.1"
                     /translation="MKRFILTITAVLFCLGIYAQEKPQKQETPSRQEEIFNKLHNERI
                     AFFTSEIDLTPEEAQLFWPVYNKYLKECQRAHVQSRKSLQALKNKSGEKLSEAEILKR
                     IDAYVASIAAEDEVFVKYAPEFKKVLPMEKVAKLYVAEEKFRIKMIRGFRNKSSDHKD
                     SENSGSVTNKGKH"
     gene            complement(5002..5751)
                     /locus_tag="PLI69_00035"
     CDS             complement(5002..5751)
                     /locus_tag="PLI69_00035"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG40911.1"
                     /translation="MGAFRFKRFALSNERSAMKVNTDGVLLAAWVNLEQTPRANKSPL
                     KVLDIGTGTGVIALVITQRREGDALVTGIDIDSDSVEEATENFANSPWSEVLTARQIS
                     FQKFAAQYEAGAFDLIISNPPYFIDSQKAPSHRRSTARHSDTLEYDDIINGAITLLAP
                     EGRLALILPMEESLIFERMAESKGLALTRLCLVRSLPDTPPKRALMEFSLRNKICGNE
                     IPVTPEELVIQTKSDFTQEYKSLTSEFYLNF"
     gene            complement(5755..6408)
                     /locus_tag="PLI69_00040"
     CDS             complement(5755..6408)
                     /locus_tag="PLI69_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG40912.1"
                     /translation="MIKADHIKKSFGTLVVLKDICFRADAAEVVSIVGASGAGKSTLL
                     HILGTLSKPDSGQVIIDGTDVLSLSDKELARFRNTRVGFVFQAHHLLPEFTAIENVMI
                     PALIAGKSRREASSGALELLSQLGLSDRVNHKPSELSGGEQQRVAIARALVNRPAVLF
                     ADEPSGNLDSKNKDELHRLFFDLRDRYGITIIIVTHDKELAALSDRTLEMKDGEFVR"
     gene            complement(6408..8525)
                     /locus_tag="PLI69_00045"
     CDS             complement(6408..8525)
                     /locus_tag="PLI69_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG40913.1"
                     /translation="MKTYSTDKIRNVVLMGGTRSGKTTFAEAMLFEGKVIDRRGTVEA
                     KNTVSDNTEVEQLFQRSIYSTPLFAEFMDHKLNIIDAPGADDFIGGVVSAMKVCDSGI
                     MLINAQQGVEVGTEIHARYAEERNFPVVLAVNQLETEKANWDNTIDSLNQAFGSKAVV
                     FQYPVNPGLGFDSFIDVLTMKLYRYKDENGTREELDIPADQVGKAEEMRAVLMEMAAE
                     SDESLMEKFFEDGELSEEDMNKGLNIGFVKGELYPIFCLSAKKCIGVKRIMQFMIDVA
                     PAPSEKVNGPISLFVYKSSVEQHLGEVSYFKVMSGKIGEAQDLVNPVTGSKERVTALY
                     AVAGKKKEKVSELVAGDLGCTVKLRSVKTNQTLNQPGYEEVVEPTVFPPSRYRAAIKA
                     KDEKDEEKLGEALNRAAAEDPTLVVEYSKELKQTILNGQGEQHINILKWHLNNTNKIE
                     IDLFAPKIPYRETITKVANATYRHRKQSGGAGQFGEVTLLVEPIVEGVETTAKFKIDG
                     KEQVLNVRAKEEFVLDWGGKWEFYNCVVGGAIDANFMPAIKKGIIQRLEEGPLTGSYA
                     RDIRVFVYDGKMHPVDSKEIAFIIAGRNAFREAFRNAGPKIMEPIYLVEVMVPGDCMG
                     DVMSDIQNRRGMIEGMSSKKGFQVLRARVPLAELYRYSTTLSSLTSGRATFTQELIEY
                     QQVPADVQEKLLKEYEAESKEED"
     gene            complement(8613..9245)
                     /locus_tag="PLI69_00050"
     CDS             complement(8613..9245)
                     /locus_tag="PLI69_00050"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOG40914.1"
                     /translation="MLSAEALLRGDYPALIEAGCDEAGRGCLAGPVFAAAVVLPKGFS
                     HPLLNDSKQLNASQREQLREIIEREALAWSVVSIDAQEIDRINILNASIEGMHRALDN
                     LKNSTGEKVIPSLIFVDGNRFKPYREIPHHCIIKGDGKLSCIAAASILAKTHRDEYMR
                     HLATQYPQYGWEENMAYPTAKHREAIALYGLTPYHRRSFNLIDNQLNLHI"
     gene            complement(9231..10316)
                     /locus_tag="PLI69_00055"
                     /pseudo
     CDS             complement(9231..10316)
                     /locus_tag="PLI69_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            10377..12518
                     /locus_tag="PLI69_00060"
     CDS             10377..12518
                     /locus_tag="PLI69_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOG40915.1"
                     /translation="MKKITIVSLLALIFAIAPMRADEGMWLLPLLKKMNINTMQQMGL
                     KLSAEEIYSINNSSIKDAIVHFGGGCTAEIISSEGLLVTNHHCGYGSIQRLSSIEHDY
                     LADGYWAMDRSQELPAEGLTVTFLESMTDVTKDVEKVLKKARTAQDSTEAMKQLEANL
                     TGKAVGENKYLRARVYSMYGGNAYYLVVSKVYNDVRFVGAPPSSIGKFGADTDNWMWP
                     RHTGDFSMFRVYADKNNNPAPYSPDNVPYRPKQHLKISLKGIQPNDFTMIIGYPGRTN
                     RFMTQSELVEQRDISNAISIYIRGIRQELMMEDMLADPKVKIQYASKYSGSSNGWKKW
                     MGMNETFAKLNVEARRAQEEKEFKEWVGKKKSRVKKYDDALDKISYAVKERTEALTIY
                     TTLAESVGRIEIMSIASMVNSFYTTPFATDKEREQYLNVLDRRVAAFYKDYSLPTDRK
                     IAKAMMKIAKERLLEENLPSFYKEIESNFAGDINAYVDYVFDNSIFTSDKKVLEAIAA
                     ETPIKEDPAVVLANSFSAVIPPLRAKIASVQKIFDEGKKAYIAGTLEMREGEAIYPDA
                     NSTMRLTYGSVLPYSPKDAVVYNYYTTLEGVMQKEDPDNWEFIVPERLKELHKAKDYG
                     KYAMPNGEMPVCFITNGDITGGNSGSPVLNARGELLGLAFDGNWEAMSGDIIFEPELQ
                     RCINVDIRYVLFIMDKFGGAGYLLDEMDIVK"
     gene            12545..12994
                     /locus_tag="PLI69_00065"
     CDS             12545..12994
                     /locus_tag="PLI69_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOG40916.1"
                     /translation="MSLERQIQEDLKAAMFAKNATALASIRGIKAAIMLAKTAPDATD
                     ELTDADIIKIIQKLVKQRKESAAIYTEQSRPELAEAELAEAAIMEKYLPVQMSEEEVE
                     EALKKIIAQVGAKSPADMGKVMGVATKALAGQADGRLISIIVKRLLS"
     gene            complement(12960..13865)
                     /locus_tag="PLI69_00070"
     CDS             complement(12960..13865)
                     /locus_tag="PLI69_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOG40917.1"
                     /translation="MNNQYNIKGPYNPNPPKKEQKKKRTILDLDQKQLAHVDKIIAIA
                     SGKGGVGKSTVAVNLAITLARMGYKVGLADADVYGPSVPKMTGTEGEVPGVELYDARG
                     NLFEDYGQYTEECTEIILPIEKFGVKWMSIGYFARPEQALIWRGPMACNALKQMILQV
                     KWGELDFLLIDLPPGTGDIHISLVHDIPLDGAIIVSTPQAVALTDVEKGVNLFRNENV
                     NKHIYGIIENMSWFTPAELPQNKYYIFGKDGCKAMSEKYNLPLLGQVPLVQSIREGGD
                     EGNPAALLDGPLFDAMKAAALQLLK"
     gene            14019..14555
                     /locus_tag="PLI69_00075"
     CDS             14019..14555
                     /locus_tag="PLI69_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG40918.1"
                     /translation="MEFLRVDIDNTERWNKVWELYEASFPIAELRKKKDHLRACQDPC
                     FFPISAWEGNQLIGLLFYWEWNTYRYLDYLAVSPELRDHGYGLQMLQYLRDTEHKVIL
                     EIDPLTDEQSIRRLHFYEHAGYTLTPYRFSLAYRLDAEPQELLIMSYPEIITKEQYND
                     FLDFIEEHVILYCEREES"
     gene            complement(14631..>15369)
                     /locus_tag="PLI69_00080"
     CDS             complement(14631..>15369)
                     /locus_tag="PLI69_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOG40919.1"
                     /translation="QVGAKGLIYIRVTEEGIKSSIDKYFSAEELQALAKDMGAKVGDM
                     ILIMSGERKKTQVALGTLRIELGNRLGLRRPDRFAPLWVYDFPLLEWDEETERFYAMH
                     HPFTSPKPEDLDKFYSNGKEDLERVAANAYDFVLNGTELGGGSIRIFDSNIQKRMFEV
                     LGMNEEEYLYKFGFMIDAFKYGAPPHGGIAFGFDRLCALFGGQETIRDFIAFPKNNTG
                     RDVMSDAPSKIDDTQMAELFLKTTAKK"
     assembly_gap    15370..15379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15380..16403)
                     /gene="aspS"
                     /locus_tag="PLI69_00085"
     CDS             complement(<15380..16403)
                     /gene="aspS"
                     /locus_tag="PLI69_00085"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOG40920.1"
                     /translation="MYRTHTCGELRLEDVGRSVTLAGWVQKTRKMGGMSFIDLRDRYG
                     ITQVVIDESSSSELNDKVSRLGREFVIQVMGRVEERESKNPRIPTGDIEIKLTHLNIL
                     NESETPPFTIEDESDGGEEIRAKYRYLDLRRNPLKRALTLRHKLAHETRNFLDSRNFI
                     EVETPFLIKSTPEGARDFVVPSRMHPGEFYALPQSPQQYKQLLMVAGYDRYFQIVRCF
                     RDEDLRADRQPEFTQIDCEMSFVEREDVLNTFEDLMKHLFKSVLKVEFKEQFPRISYN
                     DAMTYYGSDKPDIRFEMKIREITDKVKGHDFVVFDSAPYIGGIVVEGIASYTRKQIDE
                     LTEWVKR"
     gene            complement(16408..17736)
                     /locus_tag="PLI69_00090"
     CDS             complement(16408..17736)
                     /locus_tag="PLI69_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOG40921.1"
                     /translation="MRNKLFLLTLATFVAVIFTASCKRETAVEPSWYTNEPYFLEKHV
                     MYAELPVDTSNIVLVGDDFIDRGMWSDFFGSVKVIGRGITYDGTEHILYRIGGIAKAK
                     PKKIFLSAGARDIMHGDDNAAILKRVSEICSLIKKASPESEIYYIGVTPMGEMTAEQI
                     AQTAELNISLAESAEETGFTFIDMATVLTDESGAINPAYSWNGMLLNGAGYEAYAKAI
                     EPFVGLTALNKAQEEEVKAERFDHYNHRLSILRSLPNSEERSVVMLGNSLINNAPWKE
                     LFPFLNILNRGISGDVIEGIIDRVDEIADENAGKIFLMTSTNDLLNDQELPILEIWAK
                     YESLIIKLRKEMPNTILYVHSVLPLNPKTKFYEGNNERITELNKLLDAGKDRYEYIYI
                     DIASLLSDTNGDLADEYTTDGIHLSATGYFVWAKKLAEGYRIMIMEPSFE"
     gene            complement(17756..18859)
                     /gene="ychF"
                     /locus_tag="PLI69_00095"
     CDS             complement(17756..18859)
                     /gene="ychF"
                     /locus_tag="PLI69_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOG40922.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNCISSSKAQAANFPFCTIEPNIGFT
                     IVPDDRLTVLEKMVKPRRVVPTTVEIVDIAGLVKGASKGEGLGNQFLANIRETDAILH
                     VLRCFDDPNVVHMDGSVNPVRDKEIIDIELQIKDLDTVNNRIVKVQKQAQAGGDKQAK
                     KVLELLLRYKEKLEMGRNAREVVIENAEEAKIAKEFYLLTNKPVMYVCNVDESSALSG
                     NAYVDALREAVNGEDAEILVIAAKTESEIAELESFEEREMFLREIGLESSGLVRLIQS
                     AYSLLSLQTFFTVGEPEVRAWTIKKGAKALKAAGVIHSDFERGFIRAEVIKYDDYVAL
                     GSEAACRAAGKISSEGKEYVVQDGDIMHFLFNV"
     gene            18955..19974
                     /locus_tag="PLI69_00100"
     CDS             18955..19974
                     /locus_tag="PLI69_00100"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOG40923.1"
                     /translation="MRGLFLSILLIVAAPLQERAKEEIRVMFWNVENYFDPFDDEKTN
                     DEDYIYGGRYGWSWKRFIRKRNAIAKVIVSSAGEPGTWPDLVGLCEVENRMVLRQLTE
                     QTPLVRLDWGIVHYDSPDKRGIDVALLYRKERFKVLRSRAVEVSLPDSSATRDILYVC
                     GIMLQGECALDTLHLFVNHWPSKLSDDSVTSPRRMAAAEALKEAIDSVRIFSPEAAII
                     IMGDFNDTPDSRILTDFSREMGFYSPAAGLASRGQGSIKYKGVWELIDQFFLSGNLLG
                     RVIQWDDGAFMRIFAPEFLLEADKEFLGQKPKRTYIGPRYNGGVSDHLPILLRLQRKM
                     HIFAQ"
     gene            19971..20765
                     /locus_tag="PLI69_00105"
     CDS             19971..20765
                     /locus_tag="PLI69_00105"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOG40924.1"
                     /translation="MKQYLDLLEHIMRNGTDKSDRTGTGTRSVFGYQMRFDLSQGFPA
                     LTTKKLHLRSIIYELLWFIAGDTNIRYLNEHGVTIWDEWADANGDLGPIYGHQWRSWP
                     TPDGGTIDQLTTLVKSLKSNPDSRRHIVSAWNVAEVGKMALPPCHALFHFYVAGGKLS
                     CQLYQRSADVFLGVPFNIASYSLLTMMLAQVCGYIPGEFVHTFGDAHIYSNHFEQVAL
                     QLSREPRPLPKMNINPEKKDLFNFEYEDFTLVGYDPHPGIKAPIAV"
     gene            20762..21295
                     /locus_tag="PLI69_00110"
     CDS             20762..21295
                     /locus_tag="PLI69_00110"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOG40925.1"
                     /translation="MSRVPIISIIVAVATDGAIGKANTMPWHISEDMKYFKRVTLGKC
                     VIMGRKTWESLGSKPLPGRDNIVISRSLSVDDLGEIPSRLHFVTSLDSAINLALSISA
                     ESGENEVFVIGGGHIYHQALERASRVYITEVHTSVPEADTFFPVLDPEKWHIATRSGI
                     AMDEKSGLNYEFLIYEC"
     gene            21285..22652
                     /locus_tag="PLI69_00115"
     CDS             21285..22652
                     /locus_tag="PLI69_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOG40926.1"
                     /translation="MSANKSQLSSTTGRDSFTSSFGVLVALAGSAVGLGNLWRFPYLV
                     GENGGAAFIFIYIAFVFILCLPIMYSEFIIGRRSQRNVFGAFKVLAPGSKWGIVGIFS
                     VLSSLCILSFYSVVGGWSIEYLVKSITFDFTRETGGNLETMFGEFVSSPIKPLIWHVV
                     FLLLTAMVVIAGIKNGIEKYTKMMMPLLFVMIIIIAVRSVTLEGANEGIVYLFKPDFS
                     KVTFDMMLSALGQAFFSLSIGCGTIMTYASYVSKSENIVKTTALTAVADTTFAIIAGL
                     AIMPAVFAFGISPGEGPGLVFITLPKIFAQMPLGGLLAIIFFFVLLIAALTSSISLLE
                     VVVAYLKEEFKIKRTTAVIIGSSIFLVTGTLASLSMGVLSDVKIFGKTFFDLFDTLSS
                     DFFLTTCGLLVVLFVGWRLGKANVMDELTNSGTLKLPKWFTEMTFFIIRFVAPATILV
                     IMIFG"
     gene            complement(22904..24544)
                     /locus_tag="PLI69_00120"
     CDS             complement(22904..24544)
                     /locus_tag="PLI69_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40927.1"
                     /translation="MRKNIINLMLIAFVSVAMASCGNPTKMIEAADQVKINCNPEVLE
                     VIAGNIDAVVDVTFPAEYFHPKAILEVIPVLVYSGGEVEGKPFVYQGEKITDNYKLVT
                     EDGATISERIHFKYVPGMEKSQLIARATVLYKGKEFPFPADIKIADGAYTTYMLVETE
                     GAYVKMKDNYKEVIPETEEAQILYLVNSATVRPSQLKSEDIKNFEQALKDLAKDERRE
                     VKGTNIVAYASPEGAVDFNNRLSANREKSAERAFNQFAKKLETGAVSTKSLGEDWEGF
                     QELVKNSNIEDKELILRVLSMYSDSNVREREIRNMSQVYKTLAETILPELRRARFIAN
                     IEYTNYSTDELKELVETNINVLDEEALLRAATLVKDNNAKLDIYKKAIDKFDSDRAKY
                     NAACVYLDLMKNSEAKALLEKINDKKCYYKNAMGVIAMREGKNAEAMKWFNDCHRPEA
                     KINAAVIDILEGRYKEALTKLDGTGDHNEPLAHILNGQPEKAVVKCKCPHASYLRAVI
                     AARKGDAETCKAELEKASQREDYAKRMLTDIEFAKILR"
     gene            24652..25857
                     /locus_tag="PLI69_00125"
     CDS             24652..25857
                     /locus_tag="PLI69_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG40928.1"
                     /translation="MDLRQIKDKYQIIGKDPRLDRAIEAAVNVASTDLSVIVTGESGV
                     GKEFIPRIIHDHSPRKHKRYIAVNCGAIPEGTIDSELFGHEKGAFTGAVDSRKGYFEE
                     ADGGTIFLDEVAELPVGTQVRLLRVLQNGEFIRVGSSKVQKTDVRVIAATNIHLDRAV
                     AAGKFRRDLYYRLNTVPIVMPALRERKADIRMLFRKFASDFADRYTMQPVRLDDEAMQ
                     LLENYPWPGNIRQLKSVTERISIMETDRLITAEKLSAYLPKDHSSQMPVHTGSFFDSC
                     SDPYREFLIQLYAQYQQLRMDVDELVRIVKGSAPTQQVQQAPTINHTHMLTEGSEVRV
                     PGPVIEVEEVENLNEQEEGTPLTLDQITGRAIAEALHRNANNREKAAAELGISARTIY
                     RKIKEFNLE"
     gene            25866..26384
                     /locus_tag="PLI69_00130"
     CDS             25866..26384
                     /locus_tag="PLI69_00130"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOG40929.1"
                     /translation="MRRISKILLPLIALLATATLVTGCWLYSFSGTSIQPDVKTICIE
                     PVQNRALKVNPSLANSLHEALTDKYRKLTRLEQVEDAGDLYVLAVIEGYDVSATAITA
                     DEVAARNRLTVTVKVKFVNEKYPEENFEKNFAGYEDYDSDRSLDEVEATLCDTIIEKI
                     VEDIFNATVANW"
     gene            26398..26976
                     /locus_tag="PLI69_00135"
     CDS             26398..26976
                     /locus_tag="PLI69_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40930.1"
                     /translation="MKLRELSISELEEMLERYPWFAAAHREFVIRKGGAGDEELRRAA
                     AAAGIHLLSRHFFVKSVRRSIKERLYDDDVIFGKPSNAEHRLKYYVVGGDYFGQEDFK
                     ELERSGEAFKETFNINPISSTLSSLPDFDFENRNTGEDDSEQFYTETLARIYVEQEFY
                     NQALSIYEKLILLYPEKSAYFASLIENIKILK"
     gene            26984..27346
                     /gene="secG"
                     /locus_tag="PLI69_00140"
     CDS             26984..27346
                     /gene="secG"
                     /locus_tag="PLI69_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOG40931.1"
                     /translation="MQAAYVTVSIIIVILSILLTLVVLIQNSKGGGLAANFATGNQAF
                     GVRQTADFLEKITWGLAVSILVLCVVATFLIPDRNASGSKLREAIEQQVTTEGTPIVN
                     PEVAVPVELPTTPESDNN"
     gene            27503..28087
                     /locus_tag="PLI69_00145"
     CDS             27503..28087
                     /locus_tag="PLI69_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOG40932.1"
                     /translation="MKKVVNAGIGGRPFILEEDAFSKLESYLSFFKSKINMGSQEKEV
                     MDDLEMRIADLFEEKLGSGSEVVTLQMVEEIISRLGMPDGSSPFENGGDTSSSASASA
                     ESKGPRKLYRDPDDRVIGGVCSGLAQYLNIDVVLVRILFVVFTLIGGAGIWVYIILWI
                     VAPQALTAAQKCEMRGWPVTAENIRKASQSQNRK"
     gene            28093..28422
                     /locus_tag="PLI69_00150"
     CDS             28093..28422
                     /locus_tag="PLI69_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOG40933.1"
                     /translation="MEHIVDNIKSQMRKGLLEYCILLILNKKEAYASSIIEELKKANM
                     IVVEGTLYPLLIRQKNQGLLNYRWEESTQGPPRKYYMITEKGRMLISEMDLAWQEIVS
                     TIETIRK"
     gene            28419..>29023
                     /locus_tag="PLI69_00155"
     CDS             28419..>29023
                     /locus_tag="PLI69_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOG40934.1"
                     /translation="MKKVEKVSIGRYAFTLYEEAYYKASEYLDELSNYYSNREGGNEV
                     MEGIEERMAELLLEKCGPGGIVSVEMISEVISILGRPEVIENADSDGSAGSGSNLHYN
                     AKKKLFRDPSSRILGGVSSGLAAYFNIETALVRVIWVVLWFVFSSAGALTLPVFGGAF
                     IPIVYIIMWLVIPPAKTVQQRCQMRGEDGTLGDLEKNIAKGA"
     assembly_gap    29024..29033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29034..29457
                     /locus_tag="PLI69_00160"
     CDS             <29034..29457
                     /locus_tag="PLI69_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40935.1"
                     /translation="DYVGRKAKEIGNSDFWKTIGRVFSIIIGMILLIIGVSGLMVGVL
                     GLFGITFFNYTILDMGLGWLSEYAPIFALSLPVMLLKTVIMLVVLLPFLGMLYGGIQM
                     IFGFKPPKWRPGLIIFVIWMIALIALVVLGLAGIFSVG"
     gene            complement(29613..>30072)
                     /locus_tag="PLI69_00165"
     CDS             complement(29613..>30072)
                     /locus_tag="PLI69_00165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG40936.1"
                     /translation="SYRVIIAGESYGSFDKYRDQIANLVAKNHSLAERIVIFNRYIDD
                     YEVPAFFSAADLCVLPYKSATQSGITAISLHFEIPVVATRTGGLEESIEEPGIGILAD
                     EISAEGVVRAIESYFSHPYKSLFTENIRKFKETMSWKSFAKALVNFCESL"
BASE COUNT         7715 a         6878 c         7139 g         8320 t
ORIGIN      
        1 gccgaaatcg tatgcatggt cctgatgaat cagagtggag agcgcatccg ccggttctcc
       61 gttgaggaga atgtcgagtt ttaccagttt ggcttcgcga aatcccgtca gatggtagtc
      121 aaatgaggcg taacctttcg aaatggattt gagcttatcg taaaagtcga aaacaatctc
      181 cgaaagcggc atatcgtagg tcagttcgat gcggtcggaa gagatatagt gctggttttt
      241 gagagtacct ctcttttcga ggcagagctt cattatcgga ccgtaatact ccgtcttgga
      301 gataatctgc gcctggatat atggctcttc aatatggtga ataagcgttg tggggggcag
      361 tcccgaagga ttgtgcacct ctatacattc cccttgagtc gtatatactt tgtaagaaac
      421 gttgggaacg gtggtaatca catccatatc aaactcgcgg aatagcctct cctgcactat
      481 ttccatgtga agcattccaa gaaatccgca acggaatcca aatccgagcg ccagcgaact
      541 ctccggctcg aataccagcg aagcatcatt taactgaaat ttttccaaag aagcgcggag
      601 atcttcatat tcatccgcac ttaccggaaa cataccggca tagagcatgg gcttgacatc
      661 ttcaaatccg gcaatggcgc tcttacaagg attatgaaca tgtgtaatgg tatctcccac
      721 ctttacctcg gcagcagcct tgatgcccga aataatatat cccaccgaac cacattcgat
      781 ctcatttctg gggagatatt tcatctttag aactcccact tcatctgcga catactcctt
      841 gcccgtattg acgaatttga ctttatcacc tttacggata atgccgtttg ccaccttgaa
      901 ataggctatg atgcctctga aggagttgaa taccgagtcg aaaatcagag cctggagggg
      961 tgcctcagga tcgccctggg gggccggaat cttctctaca atagcctcca aaatagcttc
     1021 aatgcccaat cccatacgcg ccgaagccag caacacctcc tcatccttac agccgagaag
     1081 gtcagtcacc tgctctctga ccatatccac catggcggcc tcaacatcta ttttgtttag
     1141 gatgggaatt atctcaaggt cgtgttcgat ggcgagatag agattggaga tggtctgtgc
     1201 ctgaatacct tgtgtggcat ccaccaccag gagggcaccc tcacaggatg cgatggcgcg
     1261 tgatacctcg tatgaaaaat ctacgtgacc cggagtatca atcagaatga gagtatattt
     1321 ctcaccttta tgcacatagt ccatctggat ggcgtggctc ttgatggtga tacccttttc
     1381 tttctccaaa tccatggaat ccagcacctg tgtcgccata tcacggcgat cgatggtatt
     1441 ggtaaattcg agcagtctgt cagccagagt actcttcccg tgatctatat gagcaatgat
     1501 gcaaaaattc ctgatatgtt tcatacttgt cttcttccaa accgcaaaga taatgattat
     1561 ctttgttttt taatttcatt tcgtactatg aacgacattc atacttatca aaaaatcgca
     1621 tctcaggtca gaagggacat cctaaggatg gtcacgggag caggttccgg ccacccggga
     1681 ggttcgatga gcagtgccga cattttgacg gcactctact tcaaggagat gaatgtgact
     1741 cccgagggtt ggaacagggc cggtaacggt catgatatct tctttctctc cataggccac
     1801 atatctccta tcttttacag cgtgctggct cgcagagggt ttttccctat ttcagaactg
     1861 ggcaccttca gggcatacgg cagcaggctt cagggccatc cctcaattga tgccgctctt
     1921 ccgggagtgc atcaggcttc aggttctttg ggtcaagggc tgtcggtagc cattggagct
     1981 gctctcgcaa agcgcctcgc gggcgacaat agcagagtat ttgttcttat tggtgacgga
     2041 gagtctcaag agggtcagat ctgggaggca gcggcggcgg cagtgcacca caaggtggac
     2101 aatctgattg ccatgaccga ctggaataag cagcagatag acggcagcac cgaagaggtg
     2161 cagagcctcg gcgatttgac tctgaagtgg aatgctttcg gatgggagtg catagtggct
     2221 gacggtcacg acttcaactc catttttgag gcattcgaca gggccaactc tcttttgggc
     2281 ggaggtcgtc cggtgatgat tcttttcgag accgaaatgg gtcatggagt cgattttatg
     2341 gcaggtactc acgaatggca tggcaagagc ccttctcagg agcagtgtga acggggactt
     2401 gcccagcttg aagagacttt tggtgattat taaaaaatga gagtatgaaa aaatatatca
     2461 atacaggaaa taaagatacc cgttcgggtt ttggggcggg tctgtcacat ctcggcacca
     2521 tcaatccgaa agtggttgcg ctctgcgcag accttaccgg ttcgctcaag atggatgcct
     2581 tcgcaaaagc acatccggaa agatttttcc agtgcggcat tgccgaggca aatatgattg
     2641 gaatggcggc aggacttgca ataggcggag ccatcccctt tgcaggcaca tttgccaatt
     2701 ttgccaccgg cagggtatat gaccagatcc gtcagtcggt ttgttactcg gaaaccaatg
     2761 taaagatagc tgcttctcac gccggcatca ctctcggaga agatggagct actcatcaga
     2821 tattggaaga tatggggctt atgcggatgc ttcccaatat ggtggtgata aatccttgcg
     2881 attacaacca gaccaaggcg gccaccatag ccgcagcagc ctggcaaggt cccgtttatc
     2941 ttagattcgg acgtccggga gtacccaact tcactcctga agatcaggtg ttcgaaatag
     3001 gaaaagccct gcatctcaac gagggatcgg atgttaccgt aatcgctacc ggacacctcg
     3061 tttgggaggc tatccaggcg gctgaaattc tcgaaaaaga gggcataagc gtagacctcc
     3121 tcaatattca caccatcaaa cctctggacc acgaagcaat aatctcttca gctgtaaaga
     3181 gcggtagagt cgtctgtgcg gaagagcatc tgatagcagg agggctcggt gaggcggtag
     3241 ccggccttct cgcaagggaa catccggtgc ctatgaggtt tgtggctatc gacgaccgtt
     3301 ttggtcagag cggtaagcct gcccagttga tgtcagccta cggcctcgat acggcacata
     3361 tcgttgctgc tgtaaaagag attatcggtg taaaatagca ggaattgtgg tggtataacc
     3421 attattgatg gaagataagg atatcatagg attgtataaa tccggtcagg ccgagcgcgc
     3481 ttttaccttc attgtgcgca agtacagtga gcgcctctac tggcatatca gacagctgga
     3541 ggctgatcac gacgatgccg acgatatcct gcaggagaca tttatcaaag tatggaatgc
     3601 tcttcccgca ttcagggagg attccaagct ttacacctgg ctttaccgca tagctacaaa
     3661 tgagagcctt acatttctga agaggcgaaa actccgggcc acctttacac ttcgtgatcc
     3721 ggatatggac atggcctctt ttatagagtc cgatccgctt tttgatgggg acgaactcca
     3781 actttccctt cagaaggcta tagccaccct tcccccgaaa cagaagagtg tatttgtgat
     3841 gcgttatttc cacgagataa agtatgagga catttcagaa ataatgggca tttccgtagg
     3901 agcgttaaaa gcctcttatc atcacgcatt caacaaagta caagaatatc tcaaaaaaga
     3961 ggggtattaa accttttgcc tcttttaatg tctaaaagtt atatgaatga gcaatataac
     4021 ataactgatg atccgcactt aaagagcaat cctttcaagg cccccgaagg ttatttggaa
     4081 tctctcgagg agaggattct ttctttggcc ggcattgttc agggtgaggc ggcagaggaa
     4141 gttgtaactc cttcaggcgt ctggagacgg ttcggcattc ttaaaccggc cctcttattg
     4201 gcctgtacat tcggagttat attcggcatg gggtacggtg tattgagatt gaccggtact
     4261 cttgataagt ctccaaatgg cgaggactcc tattatgcaa aaatggtaga aaaagggttg
     4321 attaaaacta agttcatcga ttggattgaa gaggatttgg agtacgatga tttctatgaa
     4381 gaagatcagg attttgatga ggaagaagta gtcagttggt tggcaacaca gtactcgtca
     4441 atggagctgg cacagttgta ttatgacgaa aaatagaaaa gatatgaaaa gatttatttt
     4501 gactattacg gcagttctgt tttgtcttgg catatatgct caggagaagc ctcagaagca
     4561 ggagacccca tccaggcaag aggagatatt caacaagttg cataatgaaa gaattgcctt
     4621 tttcacatct gaaatagacc ttacgccgga agaggcacag ctattctggc cggtctacaa
     4681 taaatatctc aaagagtgcc aaagggccca tgtacaaagc aggaaatccc ttcaggcttt
     4741 gaaaaataag tcgggagaaa aactcagcga agccgaaatc ctgaaaagga ttgatgctta
     4801 tgtggccagt atagctgctg aggatgaggt tttcgtcaaa tatgcccccg aatttaaaaa
     4861 ggtgctccct atggagaagg tggcaaaact ttatgttgcg gaagagaaat ttcgtataaa
     4921 gatgatccgc ggattcagga acaaaagttc cgaccataag gattctgaaa actccgggtc
     4981 tgttaccaat aaggggaaac attaaaaatt gagataaaac tcagaagtaa gcgacttata
     5041 ctcttgagta aagtcgcttt ttgtttgtat caccaactcc tcaggggtaa ccggaatctc
     5101 atttccacat attttgttcc gaagggaaaa ctccatcaaa gctcttttgg gcggagtgtc
     5161 gggcagagat cttaccagac agagacgggt gagagccaaa cccttcgact ctgccattcg
     5221 ctcaaaaata agactctctt ccataggaag tatcagtgcg agccgcccct ccggagcaag
     5281 gagagtgata gctccgttga tgatatcgtc atactccaaa gtgtcactgt gtcgcgccgt
     5341 actgcgtctg tgggaaggag ccttttgtga atctatgaaa taggggggat tggagattat
     5401 cagatcgaaa gccccggcct catattgcgc ggcaaatttc tgaaaggaga tctgacgggc
     5461 tgtcaggact tcactccagg gggaattggc aaaattttct gtcgcctcct ctaccgaatc
     5521 ggagtcaatg tcgatccccg tgacaagagc atccccctca cggcgctgag ttataaccag
     5581 agctattaca cctgttccgg tgccgatatc cagcactttc aaaggggatt tattggccct
     5641 gggagtttgt tcaagattca cccatgcggc gagcaatacg ccgtcggtat tgaccttcat
     5701 tgccgacctc tcgttggaaa gggcaaatct tttaaatctg aaagccccca tcggttatct
     5761 gacaaactcc ccatccttca tctcgagggt tctgtctgag agagcagcaa gctctttgtc
     5821 atgagtgacg atgatgatgg tgatgccata cctgtctctt aggtcaaaga agagacgatg
     5881 gagttcatct ttgttcttgc tgtcgagatt accggagggc tcgtcggcaa ataagactgc
     5941 cggacgattg accagagcgc gggctatggc cacacgctgc tgctcgcctc cgctgagctc
     6001 ggagggcttg tggtttactc tatcactgag gccgagctgg ctgagcagtt ccaaagcccc
     6061 tgaggaggcc tctctgcgac tcttgccggc aatcagagcc gggatcatta cattttcaat
     6121 agccgtaaac tccggaagca gatggtgtgc ctgaaataca aatccgaccc tggtgttgcg
     6181 aaagcgggcc agttctttgt cggaaagaga gagaacatcc gtcccgtcta taatcacctg
     6241 cccgctgtcg ggtttggaaa gtgttccgag aatgtgcagc agagtagatt tgccggcacc
     6301 gctggcccct acaatactga caacttcggc tgcatctgcc cgaaaacaaa tatccttcag
     6361 gacgacaagc gtcccgaagg attttttgat atggtcggct ttgatcacta atcttcttct
     6421 ttggactcgg cttcgtattc tttgaggagt ttttcctgaa catcggcagg tacctgttga
     6481 tattcgataa gctcctgggt gaaagtagcg cggcctgaag taagtgaact taaagtagta
     6541 gagtatctgt agagttctgc aaggggaacg cgtgcgcgga gtacctggaa gcccttcttg
     6601 gagctcattc cctcgatcat gccgcgacgg ttctggatgt cactcataac gtcacccata
     6661 cagtcacccg gcaccattac ctccaccaaa tagataggct ccataatctt cggacctgca
     6721 tttctaaagg cctctctgaa agcgttacgg ccggctatga tgaaagcgat ctccttggag
     6781 tcaaccgggt gcatttttcc gtcatagacg aatacacgga tatcacgggc ataagaacct
     6841 gtaagaggtc cctcctccag tctctggata atacccttct ttatggcagg catgaagttg
     6901 gcatcaatgg cgccacctac aacgcagttg tagaactccc attttccacc ccaatccagc
     6961 acgaactcct cttttgctct cacattgaga acctgctctt tgccgtcaat cttgaacttt
     7021 gcggtagtct ccactccctc tacgataggc tctaccagta gagtaacttc accgaattga
     7081 ccggcaccac ctgactgttt tctgtgacga tatgtggcgt ttgcaacctt cgtgatggtc
     7141 tcacgatagg gaatcttcgg agcaaagagg tcaatctcaa tcttgttggt attgttgagg
     7201 tgccatttca ggatattgat atgctgctcg ccctgaccgt tgagtatggt ctgtttgagt
     7261 tctttggagt attccactac caaggtggga tcctctgctg ccgcacggtt gagagcctcg
     7321 ccgagcttct cctcatcctt ctcgtctttc gcctttatag cggcgcgata ccttgaaggc
     7381 gggaatactg tgggctcaac tacctcttca taccccggtt ggttgagggt ctggttggtc
     7441 tttacgctcc tgagctttac tgtacagcct aagtcacctg ccacaagctc gcttaccttc
     7501 tcttttttct tgcctgcaac ggcatataga gctgtaactc tctctttgct ccctgttacg
     7561 ggattaacca gatcttgtgc ttcgccgatc ttaccggaca ttaccttgaa gtaggatacc
     7621 tcaccgaggt gctgctctac ggatgatttg taaacgaaaa gcgaaatggg accgttaact
     7681 ttttccgaag gagcgggagc aacatctatc ataaattgca tgatacgctt tacgccgatg
     7741 catttctttg cagagaggca gaatatagga tagagttcac ccttgacaaa gccgatgttt
     7801 aaccctttat tcatatcctc ttcagagagt tcgccatctt cgaaaaactt ctccatcaaa
     7861 gattcgtcac tctcggcggc catttccata agaactgcgc gcatctcttc agccttaccc
     7921 acctggtcag cgggaatatc cagctcctca cgtgtgccat tttcatcttt gtacctatac
     7981 aatttcatcg tgagcacatc gataaagctg tcgaaaccaa ggccgggatt gacaggatac
     8041 tggaatacaa ctgcctttga gccgaaggcc tgattgaggc tgtcaatggt attatcccag
     8101 tttgcctttt cggtttccag ttggttgact gcaagtacta cggggaaatt gcgctcttcg
     8161 gcatagcgtg cgtgaatctc agttccgacc tcgacaccct gctgagcatt tataagcatt
     8221 ataccggagt cgcacacctt cattgccgat accacaccgc ctatgaaatc gtcggcaccc
     8281 ggtgcgtcga ttatattgag cttgtggtcc atgaattctg caaagagcgg agtggaatag
     8341 atggagcgct ggaagagctg ctcgacttcg gtgttgtcag aaacggtgtt cttggcttca
     8401 actgtgcctc tgcggtcaat gactttgcct tcgaaaagca tagcttcggc aaatgtagtc
     8461 ttgcctgacc ttgtaccacc cattagaacg acatttctga tcttgtctgt agagtaagtt
     8521 ttcatcgtat aaaatctatt tgaatatatt tatttgttta ttgcaagagt atctttctct
     8581 caaaaatagc ccacaaattt aggttttttg cattaaatat gcaaattcaa ttggttgtca
     8641 atcaggttga atgaacgacg atggtaggga gtcaggccgt aaagggcaat tgcctccctg
     8701 tgttttgcgg taggataggc catattttcc tcccatccat attggggata ttgggtcgca
     8761 aggtggcgca tatattcatc cctgtgggtt ttggccagga ttgaagcagc tgcaatgcag
     8821 ctcaatttac cgtcgccttt gataatgcag tgatgaggta tttccctgta aggtttaaat
     8881 ctgttgccgt ccacaaatat cagcgaaggt atcacctttt ctccggtgga gtttttgagg
     8941 ttgtcgagag ccctgtgcat cccctcaatg gaggcgttga ggatattgat gcggtcgatc
     9001 tcctgcgcat cgatggaaac cacgctccag gccagcgctt cgcgctctat aatctcccta
     9061 agctgctccc tctgactggc gttaagctgc tttgaatcgt ttaatagcgg atgcgagaag
     9121 ccttttggca gtactacagc agcggcaaaa acaggcccgg caaggcatcc gcgacccgct
     9181 tcgtcacaac ccgcttcgat aagtgcaggg tagtcgcccc ttagtagcgc ctctgccgat
     9241 aagatgcagc tctgcctgtc gggacaaata tccatcagtt gatagacatt gcctatttta
     9301 tgctttttga gtagctcggg gctcactctg ttttcgccgc ataccaccac ggggcgctta
     9361 tcatatttac ggcagagcga tgcgatgccg tctatcactt taccctgcaa actctgggag
     9421 tcaaatcgac cttcaccggt tataattgtc tctgcggaag caattttttc ctctatcgaa
     9481 agaatttctg aaaatagttg ccatcccggc agaagttcgg ctcggaaaaa gccgtagaaa
     9541 gctgcaccca gccctcctgc ggctcctcct cctggtatgg cagttatctt ggcggcgcgt
     9601 ttggcaaatt ctcctccaaa tgatttgagc aacatttgcg ctttggccgc atatagctcc
     9661 atacgttgct ccagtagcgg gagagtcatc cggtttgctc ctttctgcgg tgcatatatc
     9721 atggtggctc catcagggcc caataatgga ttctccacgt cacaggctac ctgaaaggag
     9781 atagtgtcgc acattcgcac aatctcctcc ggaggtatgc ttcctccttc tccccaaatg
     9841 ccgaacccca tatcgttggt ggcgctaccc ccgataccta ttatgaccct tcttgcaccc
     9901 gcctctgctg cggcacggat catcaaaccg agtccgaaag cgtcggtttt taggggatcc
     9961 ctttcctcct ctttaagcaa tgtcaacccg caacaccgag ccatctcgat aaaggcacat
    10021 agttcaccgc cgctacgaaa gagcagcatt ggggccgtga gaggcctccg gagagggtcg
    10081 cagcactcca cctcaatcag agttgcctca gatgaacttt cccttgaaag tgcggtgtac
    10141 ataatatcca gcgacccgtc cccgccatcc gccatcggga ccttttctat ctccatatct
    10201 gaaatgtgta cagtacgcat ccttattgcc tcttctatgg catctgccac tttggaggca
    10261 ctcagggagc ctttgaattt atccggtaca atcaatattt ttctttttga aaccatttct
    10321 gtacatttgt aagataaact ctaaaaataa accatttttt tgacaaattt ttaaatatga
    10381 agaaaattac aatcgtatcc ctcctcgcgc tgatttttgc gatagcaccg atgcgcgccg
    10441 acgaaggaat gtggcttctg ccattgctga aaaagatgaa tataaacaca atgcagcaga
    10501 tgggcctcaa actttcggca gaagagatct acagtatcaa caactcctcc atcaaggacg
    10561 ccattgttca ctttggaggt ggttgtactg ccgaaatcat ctcgtcggaa ggtcttttag
    10621 tgaccaatca ccactgtggt tacggttcca tacagcgtct gagctctata gagcacgatt
    10681 atctggccga tggctattgg gcgatggatc gctcacagga gctccctgca gagggtctta
    10741 ccgtgacatt cctcgagagc atgaccgatg tgaccaagga tgtagagaaa gtgctaaaaa
    10801 aagcgagaac tgctcaggat agcacggagg ccatgaagca actcgaagcc aacctcacgg
    10861 gcaaggcagt cggagagaat aaatatcttc gcgccagagt ctactcgatg tatggcggaa
    10921 atgcctatta tcttgtggtt tcaaaagtct ataatgatgt gcgttttgtg ggtgctcccc
    10981 cttcatcaat aggaaaattc ggagcagaca cggacaactg gatgtggcca cgtcacacag
    11041 gcgacttttc catgttcagg gtatatgcgg ataagaataa taatcccgcg ccttattctc
    11101 ccgacaatgt gccttacaga cccaaacagc accttaaaat ctccctcaaa ggcattcagc
    11161 ccaacgattt taccatgatt atcggctatc cgggccgcac aaaccgtttc atgacacagt
    11221 cggagcttgt ggagcagcgt gatatttcca atgccatcag catctatatc agaggtatcc
    11281 gccaggagtt gatgatggag gacatgcttg ccgaccccaa agttaaaatc cagtatgcaa
    11341 gcaaatacag cggctcttcc aatggctgga aaaaatggat ggggatgaat gagacctttg
    11401 caaaacttaa tgtagaggcg agacgcgcac aggaagagaa ggaatttaaa gagtgggtgg
    11461 gtaagaagaa gtcccgcgtt aaaaaatatg atgatgccct cgataagatc agttatgccg
    11521 taaaagagag gacagaggct cttaccatat atactacact tgcagagagt gtgggccgca
    11581 tagagataat gtccattgct tccatggtca acagttttta caccaccccc tttgcaaccg
    11641 ataaggagag ggagcaatat ctgaatgtgc tggatcgccg tgtcgccgct ttttataagg
    11701 attattccct tcccaccgac aggaagattg ctaaagcgat gatgaagata gcaaaggaac
    11761 gcctcctgga ggagaatctc ccttctttct acaaggagat tgagagtaat tttgcagggg
    11821 atatcaatgc ttatgtggat tatgttttcg acaattccat ctttacttcc gataagaaag
    11881 ttttggaggc aattgctgcc gaaactccca tcaaagagga tcccgcagtg gttttggcaa
    11941 attcattcag tgccgtcatt ccacctctca gggcaaagat tgcctctgtt cagaaaatat
    12001 ttgacgaagg taaaaaggca tatattgccg gtactctcga gatgcgtgag ggtgaggcca
    12061 tctatcccga cgccaactcc acaatgagac ttacttatgg cagcgtgcta ccctattctc
    12121 ccaaggatgc agttgtatac aactactaca caacccttga gggcgttatg cagaaggaag
    12181 accccgacaa ttgggaattt atagtacctg aaaggcttaa agaacttcat aaagccaaag
    12241 attacggcaa atatgccatg ccgaacggcg aaatgcccgt atgtttcatt acaaatggag
    12301 atatcaccgg cggtaattcc ggcagccccg tactcaatgc caggggagaa cttctcggac
    12361 tggcctttga cggtaactgg gaggcaatgt cgggtgacat aatctttgag ccggaactcc
    12421 aacgctgtat caacgtggat atccgctacg tcctctttat catggataaa tttggaggag
    12481 cgggttatct tctcgacgag atggatatag tgaaataata gaaatctata aaaaacatta
    12541 caaaatgagt ttggaaaggc agatccagga ggatcttaaa gctgcaatgt ttgccaaaaa
    12601 tgcaactgct ttggcctcca taagggggat caaggcagcc atcatgttgg caaaaacggc
    12661 accggatgcc acagatgaac ttactgatgc agacattatc aaaatcattc agaaacttgt
    12721 aaagcagcgc aaagagagcg cagccattta taccgaacag tcacgtccgg aacttgcaga
    12781 agcggaattg gctgaggctg ccattatgga gaagtatctt cccgttcaga tgagtgaaga
    12841 ggaggtagaa gaggctctca aaaagataat tgcccaggtc ggagcaaaat cgccggctga
    12901 catgggcaag gtcatgggag tggccacaaa agccctggca gggcaggcag atggccgtct
    12961 tatttcaata attgtaaagc ggctgctttc atagcatcaa agagaggtcc gtccaggagt
    13021 gcggcgggat tgccttcgtc gcctccttca cggatactct gtaccagtgg aacctgtccc
    13081 agtaggggca ggttatattt ttcagacatt gccttacagc catcttttcc gaagatgtag
    13141 tatttgttct gcggcagttc tgccggagta aaccaggaca tattctcgat aatgccgtag
    13201 atatgcttgt tgacattttc atttctgaaa agattgacac ccttttccac atcggtaaga
    13261 gctacggctt gcggggtgct gacaatgatg gcaccatcca gcgggatgtc gtgtaccagc
    13321 gaaatatgaa tatctccggt tccgggagga aggtctatca gcaaaaagtc cagttcaccc
    13381 cattttacct gaaggatcat ttgtttgagt gcattgcaag ccatcgggcc tcgccaaatc
    13441 agcgcctgtt caggtcgggc gaaatagcct atcgacatcc attttacacc gaatttttct
    13501 atcgggagta tgatctcggt gcattcttcg gtatattggc cataatcctc gaaaagattg
    13561 ccccgggcat catataattc aactcccggc acttctcctt cggtgccggt cattttcggt
    13621 actgaagggc cataaacgtc ggcatcggcg aggcccacct tgtagcccat acgggcaagt
    13681 gttattgcga ggttgactgc tacggtagat ttgccaactc cacctttgcc ggaggctatt
    13741 gcgataattt tatccacgtg ggccagctgc ttctggtcga ggtcgagaat ggtccttttc
    13801 tttttttgct cctttttagg aggattggga ttgtagggtc cctttatgtt gtactgattg
    13861 ttcatttggt cttgtaataa ttgcgcaaag ttaataaaga aaataaggaa cgacaaggag
    13921 aaagaatttg caaaaaaaat attgaatgga aaggttgtgc cattagtgat acccaaccaa
    13981 attgttgcgt atatttgtcg cctcattaca atttactcat ggaattttta cgagttgaca
    14041 tagataatac tgaaagatgg aataaagttt gggagctgta tgaggccagt tttcccatcg
    14101 ccgaactgcg caagaagaaa gaccatttgc gtgcatgtca agacccatgt ttcttcccga
    14161 tttcggcatg ggaaggaaat caattgattg gtcttctatt ttattgggag tggaacactt
    14221 accgctactt agattattta gccgtttcgc ccgagttgcg cgatcatggt tacggtcttc
    14281 agatgttgca ataccttcgc gatacggaac ataaggttat tttggaaatt gatccgctca
    14341 ctgacgaaca atccatacgc cgtttgcatt tttacgaaca cgcaggatat acccttaccc
    14401 cttatcgctt ttccctcgct taccggttgg atgctgaacc gcaggaactg cttattatga
    14461 gttatccgga gataattacc aaggagcagt acaacgattt ccttgacttt attgaagaac
    14521 acgtaatctt atattgcgaa cgggaagaat cataatcaaa acttccacac tgcacttatt
    14581 ccaaacttat tttacccccc aactactcac tagagactca atactcaaaa ctatttttta
    14641 gcggtggttt tgaggaaaag ttcggccatt tgagtgtcgt ctatcttcga aggggcgtcg
    14701 ctcatcacat ctctgccggt attgtttttg gggaacgcga taaaatccct gatggtttcc
    14761 tgtccgccga aaagggcgca gagacggtca aatccgaaag cgataccgcc gtgaggggga
    14821 gctccgtatt tgaatgcgtc tatcataaat ccaaacttgt agagatattc ctcttcattc
    14881 attccaagta cctcgaacat cctcttctgg atattggaat caaatatgcg gatggatcct
    14941 ccgccgagct cagtgccgtt taggacaaag tcgtaagcat tggctgccac ccgctccaga
    15001 tcctccttgc cgttgctata aaatttatcc aggtcttccg gcttgggaga ggtgaagggg
    15061 tggtgcatcg catagaaacg ctcagtctct tcatcccact cgagcagcgg gaaatcatag
    15121 acccagaggg gagcgaaccg atcgggacga cgaagtccga ggcggtttcc aagctctatt
    15181 ctgagagttc cgagtgccac ttgagttttc ttcctctctc ccgacataat cagtatcata
    15241 tcacctactt ttgcacccat atctttggca agggcttgta gttcctccgc agagaaatat
    15301 ttgtcaatcg aggattttat accctcttcg gttactctta tatagatgag tccttttgca
    15361 cccacctggn nnnnnnnnng tctttttacc cactcggtaa gctcgtctat ctgcttgcgt
    15421 gtgtaggagg cgataccttc caccacgatt ccaccgatat agggtgcgga gtcaaataca
    15481 acaaagtcgt gtcctttgac tttatcggtg atctcccgga ttttcatttc gaagcggata
    15541 tcgggcttgt cggaaccgta gtatgtcatg gcgtcattat agctgatacg gggaaactgc
    15601 tccttaaact ccaccttgag cacactcttg aagagatgtt tcatcaaatc ctcgaaagtg
    15661 ttgagtacat cctcacgctc cacaaaggac atttcgcagt caatctgggt aaattccggt
    15721 tgacggtctg cacgcagatc ctcatctctg aagcagcgaa caatctggaa atatctgtcg
    15781 tatccggcca ccatcaagag ttgtttgtat tgctgggggc tctggggaag cgcataaaat
    15841 tcacccgggt gcatacggct cggcactacg aagtcgcgtg ccccttcagg agtggatttg
    15901 atcaggaaag gggtctctac ttctatgaaa ttgcggctgt cgagaaagtt gcgggtctcg
    15961 tgagccagtt tgtgacgaag tgtaagagca cgtttgagag gattgcgcct caggtcgaga
    16021 tagcgatatt tcgccctgat ctcttcccct ccatccgact cgtcctctat tgtaaaggga
    16081 ggggtttcgg attcgttaag gatgttgagg tgggtgagtt tgatctctat atctccggta
    16141 ggaatgcggg gatttttgct ctctctttcc tctaccctcc ccatcacctg tattacaaat
    16201 tcgcgtccga ggcgggatac tttgtcattg agctcactcg atgaggactc gtcaatcacc
    16261 acttgtgtta tgccatagcg gtctctgagg tctatgaagc tcattcctcc cattttgcgg
    16321 gttttttgta cccatcccgc aagggtcaca ctccggccta catcttccag ccgcagttca
    16381 ccgcaagtat gtgttctgta catattatta ttcaaagctg ggttccataa tcattatcct
    16441 atacccttcg gccagttttt tagcccacac aaagtagccg gtggcactta aatgtatgcc
    16501 atcagttgtg tactcatctg caagatctcc gttagtgtcg ctcaagagcg atgcgatatc
    16561 gatatatata tattcgtatc tatcttttcc ggcatccaac aacttgttga gttcggttat
    16621 tctttcgttg ttgccttcat aaaattttgt tttcggattg aggggaagaa cgctatgcac
    16681 gtaaagtatg gtattgggca tctctttcct aagtttaatg atgagcgatt catatttagc
    16741 ccatatctcc aaaatgggga gctcctggtc gttgagaagg tcattggtag aggtcatcag
    16801 gaagattttg ccggcatttt catcagcaat ctcatcaacc ctgtcgatta tcccctcaat
    16861 gacatctccg ctgataccac ggttgaggat gttgaggaag gggaagagtt ccttccaggg
    16921 ggcattattg atgagactgt tgccaagcat tacaactgac ctttcttcgc tgttgggaag
    16981 agatcgtaag atggaaagac ggtgattgta gtggtcaaat ctttcggctt tcacctcttc
    17041 ttcctgtgcc ttattcaggg cggtcaggcc cacaaagggt tcaattgcct ttgcataggc
    17101 ctcataaccg gctccgttga gcaacatccc attccagcta tatgcgggat tgatggctcc
    17161 gctctcatcg gtaagcacgg ttgccatgtc gatgaaggta aagccggttt cctcggcaga
    17221 ttcagccaga gagatgttaa gttctgccgt ttgtgctatc tgttcggcag tcatttctcc
    17281 cataggggtg actcctatgt agtaaatctc actttcaggg gaggcttttt ttatgaggct
    17341 gcatatttca gatacacgtt ttagaattgc cgcattgtca tctccatgca tgatgtctct
    17401 tgcaccggca ctcagaaaga tttttttagg tttagctttg gctataccgc ctatcctata
    17461 tagaatatgt tcggtgccgt cgtatgtgat acctctgccg ataactttga ctgatccaaa
    17521 aaagtcactc cacatacccc tgtcaatgaa gtcgtcaccc actaacacta tattggatgt
    17581 atccaccggc aattcggcat acattacatg tttctcaagg aaatagggtt cattggtgta
    17641 ccatgagggt tccacggccg tctccctttt gcaggaagcg gtaaatatta ctgcgacaaa
    17701 tgtcgccaga gttaatagaa atagtttgtt tctcatttta tcatcatttt tgtcattata
    17761 cattgaaaag aaagtgcatt atatcgccat cctgtaccac atattctttg ccttcactgc
    17821 ttattttacc cgcagcacga caggcagctt cagatccaag tgcaacatag tcatcatatt
    17881 tgattacctc ggcacgtatg aaccctctct cgaagtcgga gtgtatgact ccggcggcct
    17941 tcaaagcctt agctcccttt ttaatggtcc atgcccttac ttcaggttct cccacggtga
    18001 agaaggtctg caggctcagt agcgaatagg cagattgtat cagacgcacc aatccggaac
    18061 tctccagccc tatctcccgc agaaacatct ctctctcctc aaagctttcc aattctgcaa
    18121 tttccgattc ggtcttggcg gcaataacca gtatctcggc atcttcacca ttgacagcct
    18181 cacgtaacgc atccacgtag gcgttacccg atagagcaga ggattcatct acgttgcaaa
    18241 catacatcac gggcttgttg gtgagcagat aaaattcctt tgcaatcttg gcctcttctg
    18301 cattctcaat cacaacttca cgggcattgc gacccatctc cagcttctcc ttatatctga
    18361 gcagcagctc caggaccttt tttgcctgtt tatcaccacc tgcctgcgct tgtttctgaa
    18421 ccttcacgat acggttgttg accgtatcca aatctttaat ttgaagctct atatctataa
    18481 tttccttgtc tcttaccgga ttaacgctac cgtccatgtg gactacgttg gggtcgtcaa
    18541 agcagcggag tacatgcaat atcgcatctg tttcacgaat attggccaga aattggttgc
    18601 cgaggccttc gcctttgctt gctcccttta caagtcctgc tatatctacg atttccacgg
    18661 tggttggtac cacgcggcgc ggtttaacca tcttttcaag tacggtaagc ctatcgtccg
    18721 gtacgatggt aaaacctata ttgggctcta tcgtacaaaa agggaaattg gctgcttgtg
    18781 cctttgagga gctgatgcag ttgaaaagag ttgactttcc aacatttggc agtcctacta
    18841 ttccacattt aaatcccata tataattatt gcaaaaagct gtaattattg ttgcagtaaa
    18901 ctgcaaaagt aataattatt tgtttatctt ggcgctaaaa caaggaggta tgtgatgaga
    18961 gggttatttt tgagcatttt gctcattgtt gcagctccgc ttcaggagcg ggcgaaagaa
    19021 gaaatcagag tgatgttttg gaatgtcgaa aattatttcg atccctttga tgatgaaaaa
    19081 accaatgatg aggattatat ttatgggggt cggtacggtt ggtcgtggaa gcggtttatt
    19141 cgtaaacgca acgccattgc caaagtcata gtctcttcag cgggtgagcc gggaacgtgg
    19201 ccggatctgg tggggttgtg cgaggtggaa aacaggatgg tgcttcgcca acttacggag
    19261 caaactcctt tggtgcgttt ggattgggga atagtacatt atgattcgcc ggacaagcgt
    19321 ggtatagatg tggcgctact ttatcgtaag gagcggttta aagtattgcg ctcgagagca
    19381 gtggaagtat ctcttcccga cagctccgcc acacgcgata ttctgtatgt atgcggtatt
    19441 atgttacagg gagagtgcgc tttggatacg ctccatctat ttgtcaacca ctggccctcc
    19501 aagttgagtg atgattcagt tacttctcca cgaagaatgg cagccgcaga ggctttgaag
    19561 gaagcaatag attcggtgag aatattttcg ccagaggcgg caataataat aatgggggat
    19621 ttcaatgaca ctcccgattc ccgcatcctt accgattttt cgagggaaat gggattctac
    19681 tccccggctg ccggtttagc ctctaggggg cagggctcca taaaatataa aggtgtctgg
    19741 gagctcatag atcaattttt cctttcagga aatcttctcg ggagggttat tcaatgggat
    19801 gacggtgcct ttatgcgcat atttgctccc gaatttttac ttgaggcaga taaagagttt
    19861 cttgggcaaa agcccaagcg aacatatatc ggaccgcgct acaacggagg ggtgagtgac
    19921 caccttccta ttttgctgcg attgcagcga aaaatgcata tatttgcaca atgaaacagt
    19981 atctagacct cctcgaacac ataatgcgta acggtaccga caaaagtgat cgtaccggta
    20041 ccggcaccag aagtgttttt ggttatcaaa tgcgctttga cctgagtcag ggttttccgg
    20101 cgctgactac caaaaaactc catctgagat ccataatata cgaactgctc tggtttatag
    20161 cgggagatac aaatatccgc tacctcaatg agcatggagt gacgatatgg gatgaatggg
    20221 cagatgccaa cggagattta ggtcccattt acggtcacca gtggcgtagt tggccgactc
    20281 ccgacggtgg cacaatagat cagttgacga cactggtcaa gtcgcttaaa agcaatcctg
    20341 actctcgtcg tcatatcgta tctgcatgga atgttgcgga ggttggaaaa atggcccttc
    20401 cgccctgtca tgcacttttc catttctatg tggcgggagg gaagctctca tgccaactct
    20461 atcaacgcag tgcagacgta ttccttggag tgcccttcaa tattgcttct tattcactgc
    20521 ttaccatgat gctggcgcag gtatgtggct atatccccgg agaatttgtc catacctttg
    20581 gagatgctca tatctacagc aatcattttg agcaggtagc tctacagctc tcccgtgagc
    20641 ctcgtccgct ccctaaaatg aatatcaacc ctgaaaagaa ggatctcttc aactttgagt
    20701 atgaggattt cactctcgta ggatacgatc cccatcccgg aataaaagcc cccatagccg
    20761 tatgagtaga gtacctatca tctccattat cgttgcagtt gcgaccgatg gagccattgg
    20821 aaaagccaac accatgccct ggcatatttc tgaggatatg aaatatttca aacgggttac
    20881 tctcggcaaa tgcgtcatta tgggtcgaaa gacatgggag tccctcggca gcaagcctct
    20941 tcccggtcgg gataatatag ttatcagccg ttctctctcc gtggatgatt taggggagat
    21001 tccttcacgg ctgcattttg tcacttcact tgatagcgcc atcaatcttg cattatctat
    21061 ttctgcagaa agcggagaga atgaggtatt tgtcatcgga ggaggtcata tctatcatca
    21121 ggcattggaa agagcatcga gagtctatat caccgaggtg catacatccg tacccgaagc
    21181 agatacattt ttcccggttc ttgatccgga gaaatggcat attgctaccc gcagcggtat
    21241 tgccatggat gaaaagtcag gtttaaacta tgaatttttg atatatgagt gctaataaat
    21301 cacagctttc aagcactacc ggacgtgact cgttcaccag ttcatttgga gtactcgttg
    21361 cactggcagg ttctgccgta ggattgggca atctgtggag gttcccatat ttagtgggag
    21421 aaaatggcgg agccgccttt atcttcattt atattgcttt cgtttttatc ctctgtcttc
    21481 cgataatgta ttcggaattt atcataggac gaagaagtca gagaaatgtt tttggggcat
    21541 tcaaagtgct tgcacccggt tccaaatggg ggatagtagg gattttttcg gtactttcat
    21601 ctctttgcat actatcgttt tatagtgttg tgggaggatg gtctatcgaa tatctggtaa
    21661 aatccataac ttttgatttt acccgtgaga ccggaggcaa tcttgaaacg atgtttggtg
    21721 aatttgtttc ctctccgata aagccgctca tctggcatgt ggtattcttg cttctgaccg
    21781 ctatggtagt gattgccgga atcaaaaatg gtattgagaa atataccaaa atgatgatgc
    21841 ccctcctttt tgtgatgata atcatcattg cggtacgctc ggtgaccctt gaaggggcaa
    21901 atgagggtat agtctatctt ttcaaacccg atttttccaa agtgactttt gatatgatgc
    21961 tctctgcgct gggccaggca tttttctcgc tttccatagg ttgcgggact ataatgacct
    22021 atgcctccta tgtcagcaaa agtgaaaata tagtaaagac gaccgcactt acagcagtag
    22081 ccgacaccac ttttgcaatc attgcaggcc tggcaataat gccggcggta tttgcctttg
    22141 ggatcagtcc gggagagggc cccggcctcg tattcattac attgccaaag atattcgcgc
    22201 agatgccact tgggggcctg ttggccatca ttttcttctt tgtgcttcta atcgccgcat
    22261 tgacctcctc tatttctctg ttggaggtgg ttgtagcata tctcaaggag gagttcaaga
    22321 ttaagcgtac cacggcagta atcatcggtt cctcaatttt ccttgtaacg ggaactctcg
    22381 cttctctctc catgggagtg ctttcggatg tcaagatatt tggaaagact ttctttgatc
    22441 tctttgacac cctttcttcc gactttttct tgaccacatg cggcctattg gttgtcctct
    22501 ttgtgggctg gagactcggc aaggccaatg tgatggatga actcaccaat agcggtactc
    22561 tcaagcttcc aaaatggttc acagaaatga cattcttcat cattcgtttc gtcgctccgg
    22621 caaccatttt ggtgataatg atttttggat agtttcgagt atgtagtgtg tagttgatgc
    22681 aagagtttga taattaagag tgaaggtaat agttattggc aaattatcat cagtatatta
    22741 cagttatttt attgtttatc tgtaatatat tacggataat tgagtattta tcctcaacat
    22801 gctacggata attaaccccg caccccgtac cccgcactgc gtacctccaa actaaaaatg
    22861 ggcagccgta tggccaccca tttttgtttg gaacttactc cgactatctg agaatcttcg
    22921 caaactctat gtctgtgagc atcctctttg cgtagtcttc tctctgagat gctttttcaa
    22981 gctctgcctt acaggtctca gcgtcgcctt tacgtgcagc tattacagct ctgaggtaag
    23041 aggcgtgagg gcatttgcac tttacaactg ccttttcagg ctgaccgttt aggatatgag
    23101 cgaggggctc gttgtggtca cctgtgccgt caagtttggt gagagcttct ttatatctgc
    23161 cttctagaat gtcaataact gcggcgttga tcttggcctc ggggcggtga caatcattga
    23221 accacttcat tgcttctgca tttttgcctt cgcgcatagc gataacaccc attgcattct
    23281 tatagtagca ttttttatca ttaatcttct caagaagagc cttggcttcg ctattcttca
    23341 taaggtcgag atagacacaa gcagcattgt atttcgcgcg gtcgctgtca aacttgtcaa
    23401 tagccttttt gtagatatcc agtttagcgt tgttgtcttt cacgagagta gctgcacgga
    23461 gaagagcctc ttcatcaagt acgttgatat tggtctccac aagctccttg agttcgtctg
    23521 tcgagtaatt ggtatactct atattggcaa tgaatcttgc acggcgaagt tccggaagaa
    23581 tagtctctgc aagtgtcttg tatacttgag acatgtttct gatctctctt tcgcgtacgt
    23641 tggaatcact gtacattgaa agaacgcgga ggataagctc tttgtcttca atatttgaat
    23701 ttttcacaag ctcctggaag ccttcccagt cctctccgag gctctttgtg cttactgcac
    23761 ctgtctcgag tttctttgca aactggttaa atgccctctc tgcactcttt tcgcgatttg
    23821 cagagagtct gttgttgaag tctacggcac cttcaggaga tgcataggca actatattag
    23881 tacccttaac ctctctgcgc tcatcttttg caaggtcttt gagagcttgc tcaaagtttt
    23941 tgatgtcctc actcttaagc tgagagggac gtacggttgc gctattcaca agatagagaa
    24001 tctgtgcctc ttcggtctcg ggaatcacct ccttatagtt gtccttcatc tttacgtatg
    24061 caccttcggt ctctacaagc atgtaggtag tgtaggcacc gtcagcaatc ttgatgtcgg
    24121 cagggaaggg gaattcttta cctttgtaga gtacggttgc acgagcgatt agttgtgatt
    24181 tctccatgcc gggaacatat ttgaaatgga tgcgttcgct gatggttgca ccatcctcgg
    24241 taacgagttt gtagttgtcg gtaatctttt cgccctgata tacaaagggc ttgccctcaa
    24301 cctctccacc actataaacc aatacgggaa tcacctcaag aattgccttt ggatggaagt
    24361 attcagcagg gaatgtaacg tcaacaacag cgtcgatgtt gcctgcaatc acttcgagaa
    24421 cttcgggatt gcaattgatt ttaacctggt cggcggcttc aatcatcttg gtaggattgc
    24481 cgcaggaagc catagctact gaaacgaaag ctatgagcat aagattgatg atgttctttc
    24541 tcataaaaat ataattgttt ttgttaaaat aattcgtcgg aaaataatta tgttttatca
    24601 tacaaatata atgataattt ttttcagata aaaatttata actttgcaat tatggattta
    24661 aggcaaatta aggataaata tcagattatc ggaaaggatc cgaggctcga cagagccatt
    24721 gaagcagcgg tcaatgtggc ttctaccgac ctctccgtga ttgtgacagg tgaaagcggc
    24781 gtaggaaaag aatttattcc caggattatt cacgatcatt ctccccgtaa acacaaacgt
    24841 tatattgcgg tgaactgcgg agctattccc gaaggaacga tagattccga acttttcggc
    24901 catgaaaagg gtgcctttac cggtgcggtt gatagccgta agggctattt cgaagaggcg
    24961 gacgggggta caatcttcct ggatgaggta gccgaactac cggtaggtac tcaggtacgc
    25021 ctgctcagag tgctgcagaa cggtgagttt ataagggtgg gttcttccaa ggtacagaag
    25081 accgatgtaa gagtaatagc agcaaccaat attcatcttg accgcgctgt ggcagcgggc
    25141 aaattcagac gcgacctcta ctaccgccta aatacggttc cgatagtgat gcctgccctc
    25201 agggagcgca aggcagatat acgtatgctc ttccgcaagt ttgcgagcga ttttgccgat
    25261 agatacacga tgcaaccggt gcgtttggat gatgaagcga tgcagcttct tgagaattat
    25321 ccgtggccgg gtaacattcg tcagctcaaa agtgttacag agcgtatttc aattatggag
    25381 acagatcgtc tgattacggc agagaagctc tccgcttatc ttcctaaaga tcattcctcc
    25441 caaatgcctg tacataccgg aagtttcttc gatagttgtt ccgacccata tagggaattt
    25501 ctcatccagc tctatgccca gtatcagcaa ttgagaatgg atgtggatga gcttgtccgg
    25561 atcgtgaaag ggagtgctcc tacgcaacag gtacaacagg ctcccaccat caatcatacc
    25621 cacatgctca ctgagggttc cgaggtgcgg gttccgggtc cggtgatcga ggttgaggag
    25681 gtggaaaatc ttaacgaaca agaggagggg actcctctca ctttggatca aattaccggc
    25741 agagcaatag cagaagcatt gcatcgcaat gcaaataacc gcgagaaagc tgctgcggaa
    25801 ctgggtatct ccgcgcgcac catttacagg aagatcaaag aattcaatct ggaataagag
    25861 atgaaatgag acgaatatcc aaaatattgc ttcctctgat cgctctttta gcgacagcta
    25921 ctctggttac aggttgctgg ctttactctt ttagcggtac atccatccag ccggatgtta
    25981 aaaccatctg tatcgaaccg gtccagaata gggcactcaa ggtcaaccct tcacttgcta
    26041 actccttaca tgaggcactt acggataaat atcgtaaact tacccgtctg gaacaggtgg
    26101 aggatgccgg ggatctctat gtgctggctg tcattgaggg ctatgatgtc tctgcaaccg
    26161 caattactgc cgatgaggtg gcagccagaa atcgtctcac cgtcaccgtg aaggttaaat
    26221 ttgtcaatga aaagtatccg gaagaaaatt ttgagaaaaa ttttgcgggg tatgaggatt
    26281 atgactcaga ccgttctctt gatgaagtag aagctacgct ttgcgatacg ataattgaaa
    26341 aaattgttga agatattttc aacgcaacag tagccaactg gtaacacccc tttatagatg
    26401 aaacttagag agctctccat atcggaactt gaagaaatgc tggaacgcta tccttggttt
    26461 gcagcagcgc acagggagtt tgtcattcgc aaaggcggag ccggtgatga ggagctgcgt
    26521 cgtgcagctg cagcagcagg aatacacctg ctcagccgtc attttttcgt caagtcggtc
    26581 aggaggtcca taaaagagcg tctatatgat gacgacgtca tatttggaaa gccatccaat
    26641 gccgagcata gactcaagta ctatgttgta ggaggggact atttcggtca ggaggatttc
    26701 aaggagctgg agcgtagcgg agaggcattt aaggagacct tcaatatcaa tcccatctca
    26761 tcgacgctga gctctctgcc cgattttgat tttgaaaata ggaataccgg ggaggatgat
    26821 tccgaacagt tttatactga aactcttgca agaatctatg tagagcagga gttttataat
    26881 caggcacttt caatttatga gaaactaatt ttgttatatc cggaaaaaag tgcttacttt
    26941 gcgtccctta tagaaaatat taaaatacta aaataaccca atcatgcaag cagcatacgt
    27001 tacagtctct attattatag ttattttgag cattctgctc accctcgtgg ttttgataca
    27061 gaattccaaa ggcggtgggc tggcagcaaa ttttgcaacg ggtaaccagg cattcggagt
    27121 tcgtcagacg gccgactttc ttgagaagat cacctggggt ctggcagttt caattttggt
    27181 actttgtgta gttgccacat tccttattcc tgacaggaat gcttcaggct caaaactaag
    27241 agaagccatt gagcagcagg tgaccactga aggtactcct atcgtaaacc ccgaagttgc
    27301 ggtccctgta gaactcccaa ccactccgga gtctgataat aattagtggg aaaggtacgt
    27361 ttaatagaaa aagtgacgat tttgtcactt ttttttaaaa aataatactt tgtattgcaa
    27421 ggtatttaaa aaaatatata tatttgcatt gcaaaattat ttgcatagcc aacaatttta
    27481 ttcaaataaa taaagagcaa ttatgaaaaa agtagtaaat gcaggtatcg gaggcagacc
    27541 tttcattctg gaagaggatg ctttcagcaa attggaatct tatctctctt tctttaaatc
    27601 taaaatcaat atgggttctc aggagaaaga ggtgatggat gacctggaaa tgaggattgc
    27661 cgatcttttt gaagagaaac tcggctccgg aagtgaagtt gtcaccctac aaatggttga
    27721 agagattatc tctcgtcttg gcatgccgga tggcagctcc ccctttgaaa atggtggtga
    27781 tacttcatcc tctgcttccg catcagcgga gagcaagggc ccccgcaaat tatacagaga
    27841 tcctgatgac cgtgttatcg ggggcgtctg tagcggtctc gcccagtacc ttaacattga
    27901 cgtcgtactt gtacgtattc tctttgtggt gtttacactt attggcggcg ccggcatctg
    27961 ggtatatatt atcctatgga tcgtagctcc ccaagctctg acggctgcgc agaaatgtga
    28021 gatgagaggg tggcctgtca ctgcggagaa tattagaaag gcttcacagt cacaaaacag
    28081 aaaataacgg ttatggaaca cattgttgac aatattaaat cacagatgag gaaaggattg
    28141 ctggaatatt gcatcctgct tatcctcaat aaaaaagagg cctatgcctc ctcgatcata
    28201 gaggaactca agaaagccaa tatgatcgtt gtggaaggga ccctttaccc tctacttata
    28261 cgccaaaaga atcaggggtt gctcaactac cgatgggaag aatctaccca gggacccccg
    28321 cgcaaatatt atatgattac agaaaaggga cgcatgctaa taagcgagat ggacctcgcc
    28381 tggcaggaaa tagtatccac aattgaaaca attcgaaaat gaaaaaagta gaaaaagtaa
    28441 gcataggcag atatgctttt accctctatg aagaggcata ttacaaggca tcggagtacc
    28501 ttgacgagct cagcaactat tattcaaata gggaaggcgg caacgaagtt atggagggca
    28561 tagaggagag aatggcagag ctgctcctgg aaaaatgtgg tcccggaggt atagtgagtg
    28621 tggagatgat atcggaagtt atctccatcc tcggaagacc cgaggtcatc gaaaatgcgg
    28681 attccgatgg atcggcaggc tccggcagca accttcatta caatgcaaag aaaaaactct
    28741 ttcgcgaccc ctcctcaagg attttgggtg gagtcagcag cggtctggct gcctatttca
    28801 atatagagac agctctggtc agagttatct gggtggttct gtggtttgtc ttctcctcag
    28861 cgggagccct gaccctcccc gtatttggcg gagccttcat acccattgta tatattataa
    28921 tgtggctggt aattccgccg gcaaagactg tgcagcagcg ttgccaaatg agaggagagg
    28981 atggcacgtt aggggatctg gaaaagaata ttgccaaggg agcnnnnnnn nnnagactat
    29041 gtgggtcgta aggcaaagga gatcggtaac tcggatttct ggaaaactat cggaagagtg
    29101 tttagtatca tcatcggcat gattttgctc atcatcggtg tttcaggcct tatggtaggc
    29161 gtgttagggc tcttcggcat aactttcttt aactatacga tactcgacat gggtttaggg
    29221 tggctatcag agtacgctcc gatatttgcc ttgagcctac ctgtaatgtt gcttaaaacc
    29281 gttataatgc tggtggttct tttgcccttc ctcggtatgt tgtatggtgg tatacagatg
    29341 attttcggct tcaaacctcc aaaatggagg cccggtctga tcattttcgt gatttggatg
    29401 attgcgctta tagcattggt agtgctgggc ttagcgggaa tcttttcggt aggatgatct
    29461 ttagtgtaaa gtataaattt attggtttaa ttaaaccttt taaaacaatt gtagtcaaag
    29521 atacgactat tcgttagttt ttcttaggtt aagtgagggg ggctgcacca attccatttc
    29581 tggtgcagcc ccctgaactt ttatatctcc gcttaaaggc tctcgcagaa gttaaccagc
    29641 gcttttgcaa aagatttcca ggacatcgtc tctttaaatt ttcggatatt ttccgtaaag
    29701 agggatttat aaggatggct gaaataggac tctatggccc taacgacgcc ttcagccgag
    29761 atttcatcag ccaatatgcc gatgcccggc tcttcaatac tctcttcgag gccacctgtc
    29821 ctggtagcaa ctaccggtat ttcgaaatga agtgatattg ctgttatacc gctctgggtc
    29881 gcacttttat agggtagaac gcataggtct gctgcggaga aaaaagccgg cacttcatag
    29941 tcatcgatat atctgttgaa tataacgatg cgctccgcca aagagtggtt ttttgcaacg
    30001 aggtttgcaa tttggtcccg gtatttgtca aaactaccat aactttctcc ggcaatgatc
    30061 actctgtaac ta
//
LOCUS       DAOSIE010000002        26252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_2063_length_26252_cov_4.602626, whole genome shotgun sequence.
ACCESSION   DAOSIE010000002 DAOSIE010000000
VERSION     DAOSIE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26252
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2063_length_26252_cov_4.602626"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PLI69_00170"
     CDS             complement(<1..999)
                     /locus_tag="PLI69_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG40937.1"
                     /translation="MTRQFFIGFVFIILMASLCSCESRSGVTAETECFKADSVYYAKG
                     FEISRFDEWIIVNVRNPWDTLKRLRRYVLIDKNKPLPDSLPEGIIIRTPVEKAVFYTS
                     VHASMVAQMGRIDAIKGVCEIQYITDSLILERLAAGKIADIGLATAPNIEKIIELDAE
                     LIIASPYENSGYGAAEKIGIPIVEAADYMENHPLGRTEWGRFYGLIFGEGERADSIFF
                     ATKERYNHLKNLATQSENKPRVLLERKYGASWIVPAGGSYIGTIHSDAGADYIFSDFK
                     GSESVHQTFESVLDRAYDADIWLFKYDTKQDYTYDILKDEYQPYSNFRPFKEKNIYI"
     gene            complement(1002..2123)
                     /locus_tag="PLI69_00175"
     CDS             complement(1002..2123)
                     /locus_tag="PLI69_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40938.1"
                     /translation="MKRTLLRLAIIAGAVVIMSSSSCLDPNNGKEPKIPLVTIGTYVL
                     NSGNFGSNDANFTAYNPYTKELNYNIFERSNAGKKLGDTAQDMIAFGSKIYIAVYNSG
                     VIFVTDLEGKVIKEIRTKAKGSSANLSPRYFTTWENSVYVTFYEGYAGKIDTTSLTVS
                     ALTKVGDNPEQIKIAGEKLYVANSGGMNYPNYGKTLSVLNPVTMDVMNEIKVADNPVF
                     LESDSKGHLYVVSMGNYNDVPSCLQVVNPNNDSVQKLDLGGLTPSFIARGPGNALYLV
                     SSSYDANWKLIADYYIFDTSKKEIYGKLIKDGTLIENPYSLSADTLLTLIYIGTSDYV
                     TNGQMYVIDAFGHMYHAFDTGGLNPITACPVTAGSQKDK"
     gene            complement(2144..4165)
                     /locus_tag="PLI69_00180"
     CDS             complement(2144..4165)
                     /locus_tag="PLI69_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG40939.1"
                     /translation="MQNSFRAFFKGVTSFVVALSLLPAPLFAQNKERDTVVVRHLEES
                     RITSTAKPSPTLQSAPVQVVDRTAIERLGVQELHEAVKNLAGVNIRDYGGIGGIKTVS
                     IRSLGAQHTGVSYDGIAVSDIQSGQVDIGRFSLDNVEMITLSIGQSDEIFMSARMFAS
                     AGTLNITSSRPVFDDCGTNVTARMKVGSFGTYNPSLLLEQRIGSRWALGANADWITSK
                     GDYPFIIHDGREPEHLLRKNSDVNTLRAEINIWGFIGASGKFSLKASGLSGERGLPGS
                     VVLYNQDAFERLWDKNGFVQASYNTAFGEKWDLKGVLKYNYAWSKYYDESQRYPLGFV
                     EDIYTQQEYYGSIATRFKPVKGVELTFAQDLSYNILDSTIPESPFPERLTSLTTLAGK
                     YQGASVTVVASLTNTYITEKVQKGTPAPDRNNLSPALSISWKPFQATNFRIRASYKMG
                     YRAPTFNDLYYLRVGNTNLRPERADQYNLGVTWSGALGKEEYGWLTATLDGYYNSVED
                     KIVARPTLFIWKMMNIGKVQIAGADLSVTSGIHLGSDHTVAISTNASYQYAVDISDPE
                     STTWRHQIPYTPRISGAANLAWSNPWVNLSYIISFVGKKYALPLNVERNLIKGYAEHT
                     VSAYRDFNLRGVSFRLQGEIINFTNKMYDIIQYYPMPGRNFRLTLKIKI"
     regulatory      complement(4204..4373)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4386..5915)
                     /locus_tag="PLI69_00185"
     CDS             complement(4386..5915)
                     /locus_tag="PLI69_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOG40940.1"
                     /translation="MSLINKAFLTYAHRMLKGLDRTRKHPFEVQMKVFRALLKGGKDT
                     AFGRDHGFSKIGSVRDFQRLVPVRDYNELYPYIERLRSGEDYVLWNEKTRFFAKSSGT
                     SSGKSKFIPITRTNLWGCHYGGMQRMLANYVQRFPDSRIYSGKALTLGGSVEPDLGGK
                     SFSGDLSAILLRNSPAPAEFFRVPDKKTAMMHDFHEKVEIICRKYSKANVTSFAGVPS
                     WNLIMLRRIMEYNNASRLTDVWPNMELFMHGGISFEPYRAQYDAIIPPGSVRYFENYN
                     ASEGYFAFQDDPEDKSMLLTLDNGVFYEFATLKELSEAQENERPVPTRTVEEVEPGVP
                     YAVIISTTAGLWRYLIGDVVTFTSLVPHKVVISGRTQLFINAFGEELMIGNANTALAA
                     ACKLHNATISDFTVAPIFIEGRLKGAHQWVIEFENAPEDTEAFADTLDEELCACNSDY
                     EAKRTHTMTMERLHLAVVPAGTFYRWMESRGKIGGQNKVPRLYSNRRYVDEILGTIFF
                     F"
     gene            complement(5938..6675)
                     /gene="recO"
                     /locus_tag="PLI69_00190"
     CDS             complement(5938..6675)
                     /gene="recO"
                     /locus_tag="PLI69_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOG40941.1"
                     /translation="MSLVCKSQTIVLHLTKYGDSGLIAHVLDNRLGRRGIYLRGIKKS
                     KNSAVIAQFHNLAILEVVTAASTKSKLLYMQEYHPLVQLHSIRTDISKSAIALFVSEV
                     IYRTLREESGDEELFEWLKGMISALEALEGNSANFHLKFLVGFCRQMGFLPHDNYSEQ
                     TPLFDIISARYVSAGALRDSTYLFTEQDSLLLYRLLNADLNEAMQIPLNARARSGFCD
                     QMLKYLSYWLDTPINIRSLAVLHTIFA"
     gene            complement(6645..7235)
                     /gene="rdgB"
                     /locus_tag="PLI69_00195"
     CDS             complement(6645..7235)
                     /gene="rdgB"
                     /locus_tag="PLI69_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOG40942.1"
                     /translation="MEIVFATGNANKLAEAQRVLGKRFQLVMPSELGFDGELPETHDT
                     LYENAVEKALFIWNKFQKNCFADDTGLEVDALGGAPGVHSARYAGEPKSPPNNIRKLL
                     KELEGVPYEKRTARFRCVIALIEDGHLHTFEGICEGHITTTLHGFDGFGYDPIFRPLG
                     MDATMAELSLEEKNAISHRGKALEKLLCHLSANPKR"
     gene            7324..8934
                     /locus_tag="PLI69_00200"
     CDS             7324..8934
                     /locus_tag="PLI69_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG40943.1"
                     /translation="MKIFKTIAVTIAILASVTSFAQSAEFKLGQNMEIQYSILKELSK
                     SYVDTIEYGKIIPIGIQAMLQSLDPYTTYIPEEEGEDFDMMTTGAYGGIGSLIKKSPD
                     SGVLIVEPYSGSPAIKAGIVPGDVIIAIDGVPVYGETSAQSAAKMKGQPGTEVVFTIV
                     RGRTLDTVDITVTRERIRVSDIVYSGIIRDSIGYIALTGFTEKMHLQFKESVLDLKKR
                     GAKRLVIDLRGNGGGIMEEAVSLMSLFVPEGTLVLSSRGREERMNEEYYTTTSPIDTL
                     IPVLVMVDSGSASSAEIVAGAFQDLDRGAIAGRRTFGKGLIQSIKPTLYDGKVKITTG
                     KYYTPSGRCVQAIDYSHRNEDGSVGNVPDSLKREFKTRLGRSVYDGGGITPDFITQTR
                     SYSRPAYSLVYNDILGDYAIEYFKKNLSIVPASEFHLTDEEYEDFIRYAGTREFDIRS
                     SAESSLDQLVKAAKQDGLYDLYKSEIDALSARIKVDKETMLRIKKDEILPLLEEEIVV
                     KYYMRGSEYEIRLRDDQGLYQALDWWVE"
     gene            9118..10551
                     /locus_tag="PLI69_00205"
     CDS             9118..10551
                     /locus_tag="PLI69_00205"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOG40944.1"
                     /translation="MSYIKRFMLLGLVFLAVLSACQKEVSCTLSESNIELAYTGTPLK
                     VYLSSSVPWKIEVPTDAQWLQITPLSGPAGEEIELTLKAQPNEKRVRKTQLTVRCGSR
                     ILDLSVLQQPLNVEEYTYTVLQRHTQGTYPNEVIILGDGYSALDYGSEGPFNKDAHDA
                     MEAFFSVEPFSTYRSYFRVAQFVVFSPQSGITERDKKTEVRTAFGVRFDEPYVMTMDD
                     PNLVFKAAMRIPGMTTERISNTLFILLANVDRYGGLCWQYGDGSAIALCPVCRTSAHG
                     GYALFADLIHHEAGGHGFGRLVDEYITHPGETLPDEATSASPHRHKKELQGRKYFGYA
                     FNVDISGNKEQAPWAHYYTLPGYEAVGYYEGAYFYEKGAWRPEQRSCMVDNRPYYNAP
                     SRESIVKLLLRRAAGVRLYDYLYQSSSGTYEVVPIPNDPFNMEDFMRNDMKKAWSAPM
                     PIPSDTRSGQSFIPTQPVRFIPGSPFD"
     gene            complement(10673..11005)
                     /locus_tag="PLI69_00210"
     CDS             complement(10673..11005)
                     /locus_tag="PLI69_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOG40945.1"
                     /translation="MGKFEITLRKNGEFQFNLKASNGEIILTSEGYKTKSACLNGVES
                     VKKNSVEEARYEKKVAANGKPFFNLKATNGQVIGNSQMYASERTRDNGIASVMKHAPE
                     AKIVELLD"
     gene            11110..12150
                     /locus_tag="PLI69_00215"
     CDS             11110..12150
                     /locus_tag="PLI69_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG40946.1"
                     /translation="MKRKSYIFFSLILCVLCIAETQSLSAQTPSGVMVKHVPTELIQN
                     PDNPILLMFTASWCAPCHRMRSVMFKKENISLLLRKYNVILLDVDSQPGSDLQRIYCE
                     DSAVPQFIVMDKEQNVLRKMRGATDSESEFAEFLRSGIEEIRPGLPAVQDIISQEEME
                     LLTGDDFKAATSRLKAKWSFGVGIGGHISNMPVSSRSLFAEERASALDSTQLNYRGVK
                     PGGSISALVRYTFYKSTIETGLLFMSAGGRDIRTKQSFDSYYIGVPVDFHFGIWDNLK
                     FGAGISAMYMVKDVAKNRFDAGLRLKLIWDASPFCISAGWQRGLVNILGAESPLRAKN
                     NHFFVNFAFYFN"
     gene            12159..13088
                     /locus_tag="PLI69_00220"
     CDS             12159..13088
                     /locus_tag="PLI69_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG40947.1"
                     /translation="MRVRHLFRFFIVTTLWAFGSMLFSATAAPEGSLFADGEKRVAEF
                     THDELLSRNMPAELAFLPEAISNLSMPGNRAGIWALDALDAVRHGLKVDLTHDQRYDD
                     TLCTRAALSCLQEYYDRYREWDLALAAYLHSPAVLNACLQSGDSTLLLHTLTLSDLGR
                     MEKSDHSSPAEEMYTLYFDRPISLEAVTHLTGISAKEFSGTNPIFRSDPLVPLEGYGL
                     KFPPYVAQTIEVDSLYAVTDRMVAAREAELLKKQKEAEEERKQTSITYTVKYGDTLSG
                     IAQKHRVRVSDIKKWNKLRSDFIRERQKLIIYK"
     gene            13095..14624
                     /locus_tag="PLI69_00225"
     CDS             13095..14624
                     /locus_tag="PLI69_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40948.1"
                     /translation="MKASKIFLFILSVIFILGVVWAIFPQGGIEVGGTTLRFASYEEA
                     ASGINEEVVDVDKVLSDVEQSFSMLSGSQQDTLRYFRNFLKYNPNRIWLPNDDYTYLD
                     DLFHLFESAKKENKTYRIMHYGDSQIEMDRISSIIREHLQGIFGGNGNGLVPAIQSVA
                     TYSLVQSASGALTRYTLIGDSTTVRADHNRYGVMTQLSQLSGEASITFRVSKNSNCQE
                     RAKKFSKVALLVGNTSPGFKASLRCDTIRTQNKVLKNAVNGVSLLTWELPAYASRGTI
                     TLSGSGEIYGFLMDGKNGIALDNAPLRGSSGTIFTRMSKSVMKQSFDLLDTRMIILQF
                     GGNSAVSIRNEKQISAYLRKLENQIDYFHEVAPEAQVLFIGPSDMGRSVEGRVATIPM
                     LPALNDSLKVMALSKGAAYWDLFNMMGGENSMVRWVKHSPALASSDYIHFTTKGAEHV
                     GMAFARAFEVCYNFYKLRKELDPEMVSAYMSSQDSTEVPFADTKESDINMVSEGELGR
                     E"
     gene            14621..15913
                     /locus_tag="PLI69_00230"
     CDS             14621..15913
                     /locus_tag="PLI69_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOG40949.1"
                     /translation="MRRGTLVFRFFVAAIVLFAAWSGDAFAQGLHKPIPNYMFAHFEE
                     NAITFPSGDSADFERFFLKLDTLLFFGEGDINIVHIGGSHVQSGTFPNRMRRNLLAMR
                     YGIEGGRGVVFPFSVAKSNTPSSYYSSYTGKWKAYRNSQPKTPLRMGLTGMAAATSDP
                     SATITIVSRERNPMGSPHDFRFDRVQVLGRADNDSLVPVVRLDTLTLLPLSHDPDRDL
                     YEFVLPYLTDSLVLGTSGVPGEWNLTGIVLDHGRPGLTYHGIGVNGASLNSYFKCEDF
                     ERDLALLKPDLIIFAIGINDANTSNFNKELFISNYIKMVDLARRVAPDCAILFVTNND
                     SFRRSRRSYYLNQNGPVVQDAFYSLARRYDAGVWDLFRIMGGLNSMKQWESAGLANRD
                     KVHFTEKGYILIGDLLYNALLDRYLEHLKSNTSKNGTR"
     gene            15910..17823
                     /locus_tag="PLI69_00235"
     CDS             15910..17823
                     /locus_tag="PLI69_00235"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOG40950.1"
                     /translation="MSGFIHIAADFIKGLFTFDQSHPLLFTQFYFWAFFAIVFAFFAL
                     FKNKLLLRNGFLFFASLFFYYKTSGIFTLILLFVTLYNFLGAKWMHTRKSDGGKRAVL
                     VVSLIVNLFTLCYFKYAYFITDVVNNLFGTEFVVFDLFAVVGNAIVGANRFSIDTIIL
                     PVGISFYTFQAISYIMDVYRSRVTPVRNILDFGFYLSFFPQLVAGPIVRASEFIPQLH
                     RKFFLSRRQFGIAVFWIVNGLVKKIVLSDYLAINFIDRVFENPLLYSGFENVMALFGY
                     SLQVYADFSGYTDIAIGVAMLMGFYLPQNFDSPYKAKSPVEFWRRWHMSLSRWLKDYL
                     YIPLGGNRNATFGTYCCIVSISLIGVLLSGSWWVALVVAAIAVALILIARLKPAKRKS
                     ITTNLNSMNTMLLGGLWHGASWNFMIWGGLNGIGMVIYKVWQNWGIGLRFLVITAVTV
                     TLGILSVVAPRPLYNMFFVWMAVVMTGNLVKLLYVLICRIRGIQAAIVAGPEASKESR
                     FRRLSDIWAIFQTFVFITFTRLFFRSGSNLDPAEANETAWNTAKNMVNRIGGHWDMAM
                     IPEMVWQYRNVFLLVVIGMVIHWLPDRFKRRYRLWFASIPLPLMALMVALAVFFVYQF
                     ITADLQSFIYFQF"
     gene            complement(17820..18239)
                     /locus_tag="PLI69_00240"
     CDS             complement(17820..18239)
                     /locus_tag="PLI69_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40951.1"
                     /translation="MRDKKTILASAVATVTAFVMLAQFVSCEKYVLPELTISRDTIIF
                     NYNIDSASLDIKSNVIWEIETPDISFSNQWVIVRPNWADGDTTVIFKTTLNEGPRRET
                     KARVFSETIERFLILIQEAAPEDPEDPGNQNGDPVTF"
     gene            18344..18952
                     /locus_tag="PLI69_00245"
     CDS             18344..18952
                     /locus_tag="PLI69_00245"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOG40952.1"
                     /translation="MRNIIPIIVAVALIATACHKVVDPEISVDPYAITCDYNGGSFDV
                     NIRCNVDWTADCDTLGITISPTSGTKDGSIRISVPSTTSKSTQVARITFTAKGEESSK
                     IAKTVVTIGSYPFIGVDVGQLSIAAEGGGTQFLLTANRQWSATSSNPAFTFSPENGAV
                     NDIIRVTADVNNTGSPRECIINFKLKEYQNKTAQIKIVQTSL"
     gene            18957..21905
                     /locus_tag="PLI69_00250"
     CDS             18957..21905
                     /locus_tag="PLI69_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG40953.1"
                     /translation="MRKALLIFFASLFFFPAFAQKYQFSGKVLEKGTDVPVEFATVVM
                     ADKELWAVANAKGEFVINGVSRGKTKVEISCLGYAPTTFEIEITKDLKGVTYYLSVDD
                     LTLESAVVTAKENTNSATTSRQIDRTALEHMQMVNISDISSLLPGGVSTSTGKLTNST
                     EALQIRSDETSAVASFGTAVELDGVRLSNNAAYSSVSGVSTRNIASSNVESIEIITGV
                     PSVEHGDMTNGIVKINTRKGKSPWMITAMTSPNTKQLSLSKGFDLGSKGGVINASAER
                     TTSVQDLASPYTTYQRNAFSLLYSNSFGTLGQPLRFSFGLSGNVGGYDRSADPDSFMN
                     TYSKSKENTIRSNFSLNWLLNKSWITNIELTGSLVYSDNFSSYSDNRSSSSSTSSLHG
                     MEEGYFVSTPYSSNPNAEVILRPAGYWYEVSHGDNKPLDYNLHLKINWAHDFGGIVNK
                     LKLGLHFTGSGNLGEGFYYEDLSKAPDWRPYPYKDIPFVNNLAAYGEENINIPIGNTT
                     LNLVGGVRSEWTMINNSGYGTVNSLSPRFNAKYSIIKYRRSRILRELSVRGSWGVAVK
                     LPSVSILYPTPSYYGKQVFAPGTTADGSSFYAYYILPRQIEYNPDLRWQKAQQSEVGV
                     EMDIAGIKFSLAAFYSKTLDSYFTYSEYEPFTYKWTDQRNLEKKCPIPSSDRLYSIDR
                     ETGVVTVKDITGKLPDQTLEYTEKNTFVSKTKVTNEVSPTIRQGIEWVIDFGQIQALR
                     TSIRMDGTLYKYRSVNENIVQSYNSSRTMADGITPYQFIGYYVGSSSGTSNGSESVKL
                     RNNVTLTTHIPQARLIFTLRLESSLYRYDRNLAEYSGGQIAWPMSGNEKIPSGTEFYD
                     IRGHVVKYPLYYSTFEEPNILIPFLEEYLWAKENNKKLFSELDAMINKTGYTHLFLKN
                     VFSPYFAANISVTKEIGKIASLSFYANNFFYSTMRFKMSASGTDASLFESTSYIPKFY
                     YGLTLRLKIQ"
     gene            21924..23276
                     /locus_tag="PLI69_00255"
     CDS             21924..23276
                     /locus_tag="PLI69_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4876 domain-containing protein"
                     /protein_id="HOG40954.1"
                     /translation="MKKIVSFLMFSVIFLAAVSCHKDDPYKLYDVKVQLVYAQGSDFG
                     VVADVTVSAVNTNTGMIYVGTTNSEGLAIVPAVAGLYEISATETRAEGGTAYSFNGFK
                     QQVAITHDYEKNPFVVTIDMVGSEAKQVIIKELYTGGCQKDDGSGNFQMDKYVVLYNN
                     SPVDADLKNLCFAMTIQYNSHTTNADVVDGKLTYEAQGTVPAGNGFWTWAEGQAPKLK
                     PGEEVVVAINGAINHTITYSKSVDLSKSAYYAMFDPESGYNHASYYPTPSEAIPTSHY
                     LKAYKYGAGTGWPISATGPAFFVFITPDGVTPQSFFEDTSNENFYNNLSAMKRKWVPA
                     DWVVDAAEVFSAGYVGKNNKRLITAVDGGSVVLTSKMGYSIYRNVNKDATEAIPGNKG
                     KIVYNYSMGTSDLEEGTTDPSGINAEASIKAGAHIIFMDTNNSTNDFHQRKEAALRSK
                     "
     gene            23289..24884
                     /locus_tag="PLI69_00260"
     CDS             23289..24884
                     /locus_tag="PLI69_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40955.1"
                     /translation="MKRYLTIVTMVLLCLISGRAQVTPKHFYDFDFIKGSEPWLTSSN
                     ASGLIRLPVEKIFITQAGFDKNNGGFVAHHQSDNSFNAGALAESFSHLGQDVYVYGRL
                     SYDYFKGKNMGGSVMSDPYHSPFNLVAMADSTAGVKTLEVYNLAGGVAYRFNDALAAG
                     GKINYTSGNYSKARDPRHVNMIMDMEVTAGLTWNIGNKVIIGADYIFRRNLESFYYQV
                     YGTTDKVYYYLVDYGGFFGQRELFGERGYTTQTTTVPLLTTYHGGALQLEMAFSPGVK
                     LFNEFSFKAFDGYYGKKSVTTVMYSAHNGYQAGYNGHFSFGKDNNRHIVKLTADYTKM
                     INNENVFQEKTTEGVKEIIYFGSNEALDRDLIEGNLEYSLNLGIRDHRPKLVLKASAD
                     ALYRTQTASLYPYYRKQDLFAIDGLLSGAYNIFRENGIMGIRLGAGFGGGSGSPFNDG
                     LYMPTNNPRTPRTMDNAMRMEFEYLTAYRINAGAGFRYSHFFKNNITGYVDLDYRFVN
                     ALGISYLAGAMHHRISAIVGISF"
     gene            24896..>26252
                     /locus_tag="PLI69_00265"
     CDS             24896..>26252
                     /locus_tag="PLI69_00265"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOG40956.1"
                     /translation="MIKKPILAVMVLFLAAVLCRADEGMWLINLIEQGLAKKMQERGL
                     QLDPNTLYDEEKGGLSDAVVSLAFGCSGSMISSEGLMITNHHCAYSDVHALSTPEKNY
                     LEDGFWAMERSQEIPVKKDGIWFLKKVIDVTAEVQHLRDSIVPRGGGFAMRRVYSLIE
                     GKYSKIFEGKGEVSCASMWGGNKYYMALYEVYKDVRLVAAPPICIASYGGEVDNWEWP
                     QHKGDFALYRIYTAPDGSPADYSPDNIPMKPTRTLKISTAGVQRGDFAMVIGYPGSTD
                     RYASSFKVNNTATVVNPIQVRIQGEQMKIIDGWMNLDPAVRLKYADHYFSLSNVQEIR
                     EGEIFCYTKYDVVGKKREQEVELHRWIMSDSLRAQRYGTLLTDLQIKYKTLEYIETQI
                     GYFRETMARGVRLHRMINNVARAAELLAKRKGYQVPIADSTAALKAIMDSYGEFDMRV
                     EK"
BASE COUNT         6811 a         5916 c         6320 g         7205 t
ORIGIN      
        1 gatataaatg ttcttctcct tgaatggtct gaaattgctg taaggctgat attcatcttt
       61 cagaatatcg tatgtgtaat cctgtttagt gtcatatttg aaaagccaaa tatccgcatc
      121 ataagctctg tcaagaacac tttcaaaggt ctggtgtaca ctttcgctcc ctttgaagtc
      181 gctgaaaatg taatcggctc cggcatcgga gtgtattgtg cctatatagc tccctcctgc
      241 gggaacaatc caggatgctc catattttct ctccagcaat acccggggtt tgttttcgga
      301 ttgtgtcgcc agatttttca aatggttgta acgctctttt gtcgcaaaga aaatggagtc
      361 cgccctctct ccctctccga aaataagacc gtagaagcga ccccactcag ttctaccgag
      421 ggggtggttt tccatatagt cggccgcttc cactatcgga atgcctattt tctcggcagc
      481 gccgtaaccg ctgttttcgt aaggagatgc aatgataagt tctgcgtcca gttctatgat
      541 tttctcaata ttgggtgccg ttgcaagacc tatatcggct attttgccag ccgcaagtct
      601 ttcgaggatt aggctgtcgg tgatatattg tatttcacaa actcctttaa tggcatctat
      661 acggcccatt tgtgcaacca ttgaggcatg gacggaggtg tagaacactg ccttttcgac
      721 aggagtccta atgatgatgc cttcgggaag ggaatccggt aatggtttgt ttttatcaat
      781 caacacatag cgtctgagcc tctttagagt atcccagggg ttgcggacat tcacgattat
      841 ccactcatca aaacgggaaa tctcaaagcc tttggcgtaa tagacgctgt cggctttgaa
      901 acattccgtt tcggctgtta ctccgctcct gctttcacag gagcagagcg aagccatcaa
      961 tataataaaa acaaaaccta tgaagaattg tcgggtcact cttatttatc tttttgactg
     1021 ccggccgtta ccggacaagc ggtgatgggg ttgagtcctc cggtgtcaaa tgcgtggtac
     1081 atgtgcccaa aggcatcaat tacatacatc tgtccgttgg taacatagtc ggatgtgcct
     1141 atgtaaataa gtgtaagcag tgtgtcggct gaaagggaat aagggttttc gatgagcgtt
     1201 ccgtctttta tcagtttgcc gtaaatttcc tttttactcg tgtcgaaaat ataatagtcg
     1261 gctatcagtt tccaatttgc atcataactc gagcttacaa gatagagggc gttacccgga
     1321 ccccgtgcaa taaatgaagg tgtaaggcct cccaaatcga gtttttgtac ggaatcatta
     1381 tttgggttga caacctgaag gcaggaggga acatcattat agttgcccat tgagacaaca
     1441 tagagatgtc ccttcgagtc ggattcgaga aataccggat tatccgccac cttgatttcg
     1501 ttcataacat ccattgttac ggggttgaga acggaaagag tcttgccata gttgggataa
     1561 ttcattccgc ccgaattggc aacatagagt ttttcccctg caattttaat ttgctctggg
     1621 ttgtccccaa cctttgtgag tgcggaaacc gtgagggatg tggtgtcgat cttgcctgca
     1681 taaccctcat agaaggtcac atagacacta ttttcccagg tagtgaagta gcgtggagaa
     1741 agattggcag aggaaccttt ggctttagtt cttatctcct ttatcacttt tccctccagg
     1801 tcggtaacga aaattacacc agagttgtag acggcgatat aaattttgct gccgaaagct
     1861 atcatatcct gagcggtatc accaagtttt ttacccgcat tgctgcgttc gaagatgttg
     1921 taattgagtt ccttggtgta aggattgtaa gcggtgaagt tggcatcgtt tgagccgaag
     1981 ttgccgctat tgagtacgta ggtgccgata gtaaccaaag ggattttcgg ctccttacca
     2041 ttgttggggt cgaggcagga ggaggatgac atgatcacta ccgctcctgc aatgattgcg
     2101 agtctgagaa gtgttctttt cattttttat atcgtttttt gatttatatt tttattttta
     2161 atgtgagtct gaagttgcgt ccgggcatcg gataatattg aattatatcg tacattttat
     2221 ttgtgaagtt aataatttct ccttggagtc gaaaagagac tccgcgcagg ttaaagtccc
     2281 tgtatgcgga gacggtatgc tcggcatagc cttttataag gttgcgctcc acattgagag
     2341 gcagtgcata tttcttccct acaaaggaaa ttatgtagct cagattaacc caggggttac
     2401 tccaggcaag attggctgcc cccgaaatgc gtggagtata gggaatctgg tggcgccagg
     2461 tagtgctttc agggtctgaa atatccaccg catattgata tgaagcgttg gtcgaaattg
     2521 caacggtatg gtcgctgccg aggtgaatgc ctgaagttac gctcagatcc gctccggcta
     2581 tctgcacttt gcctatattc atcattttcc aaataaagag ggtaggtctg gccacaatct
     2641 tatcctcaac gctattgtag taaccgtcca gagtggcggt gagccatcca tattcctctt
     2701 ttccgagagc gccgctccag gtcaccccga gattgtattg gtcggcacgc tccggtctga
     2761 ggttggtatt gcctactctc aaatagtaga ggtcattgaa ggtaggtgcg cggtatccca
     2821 ttttgtacga ggctctaatt cggaaattag tggcctggaa gggcttccag gagatgctca
     2881 gggccggtga aaggttgttt ctgtcgggcg caggagtacc cttttgcacc ttttcggtaa
     2941 tatatgtatt tgttagtgat gcgactacgg ttacagatgc tccctgatat tttcccgcaa
     3001 gagtggtgag tgaggtcaac ctctcgggga atggagactc gggtatggtt gagtccagaa
     3061 tattgtaaga tagatcctgg gcaaatgtca attccactcc ctttaccggc ttgaaacggg
     3121 tggctataga gccataatac tcctgttgag tataaatgtc ttcaacaaat cccaaaggat
     3181 atcgctgact ttcatcgtaa tatttgctcc atgcatagtt gtattttagt acacctttaa
     3241 ggtcccattt ttcgccgaag gcggtattat aggaagcctg cacgaaaccg tttttgtccc
     3301 acagacgctc aaaagcatcc tgattgtaaa gaaccacaga gccgggaagt cctctttctc
     3361 ccgatagtcc gcttgccttg agggaaaatt tgccggaggc gccgataaag ccccaaatat
     3421 tgatttccgc tctgagggta ttgacgtcgc tgttttttct caacagatgc tccggctccc
     3481 tgccgtcatg gatgatgaag ggataatcgc ccttggaggt aatccaatcg gcattcgctc
     3541 cgagcgccca cctgcttccg attctttgct ccaatagcag ggaagggttg taggtaccga
     3601 atgaaccaac tttcatacgt gcggtgacat tagtgccgca atcgtcgaaa accggtcttg
     3661 acgaggtgat attgagcgtc cctgcggagg caaacatcct tgcagacatg aatatttcat
     3721 cgctttggcc gatggagaga gtgatcatct ccacattgtc cagcgaaaac cttccgatgt
     3781 ccacctggcc gctctgtatg tcggagaccg caatgccatc ataggagacg ccggtatgct
     3841 gagctcccag acttcttatg gagaccgtct tgatgccgcc tatcccacca tagtcccgaa
     3901 tattgacgcc tgcgaggttt ttaaccgctt cgtgcagctc ttgtactccc aaacgttcaa
     3961 tagccgtacg atccactacc tgcacgggtg cgctttgaag ggtgggtgat ggcttggcag
     4021 tagatgtaat gcgtgattct tccaggtgac gcactaccac cgtgtcccgc tccttatttt
     4081 gggcaaaaag aggcgccggc agcagagata atgccaccac aaacgaggtt acacctttga
     4141 aaaatgccct gaaggagttt tgcatttaaa aaacaaatca tgaaatccaa acccgggatt
     4201 tccaatccaa caaataatcg gggcaggtct tctgacttgt tcctccttcg agccgccttc
     4261 ccatccgcta aggacagtgg caaagaatag cttaggaatt acagaactca cagctacggg
     4321 tatagttccg gattcacacc ggattccctt tagcctcaat tacgatgcaa aggtaaagga
     4381 tttttttaaa agaaaaaaat agttccaagg atttcatcca cataccggcg gttgctgtag
     4441 aggcggggga ctttgttttg gccgccgatt ttgccgcgtg actccatcca gcggtagaat
     4501 gttcctgcgg ggacaacggc caggtggagt cgttccattg tcatggtatg agtacgtttg
     4561 gcctcatagt ccgaattgca ggcgcagagt tcctcatcca gagtatctgc aaaggcctcc
     4621 gtatcttctg gggcattttc aaactcaatc acccattggt gggcgccctt caacctccct
     4681 tctataaaga tcggggcaac tgtaaagtca cttatggtgg cattgtggag tttgcaggca
     4741 gcagccagag ccgtgttggc attgccaatc atcaattcct ctccgaaggc attgataaag
     4801 agttgggtcc tgccggaaat caccactttg tggggaacga gtgaagtgaa tgtcacaaca
     4861 tcgcctatca gatagcgcca cagaccggcc gtagtagata ttatgacggc atagggtacg
     4921 cccggctcca cttcttcaac tgtacgagta ggcacgggac gctcattttc ctgagcttcc
     4981 gagagctctt tgagagttgc aaactcatag aagacaccat tgtcaagcgt cagcagcatg
     5041 gatttatctt cagggtcatc ctgaaaagca aaatatccct ctgaagcatt gtaattttca
     5101 aaataacgca ccgagccggg aggaataatt gcatcatact gcgctctgta tggctcaaaa
     5161 ctaatgcccc cgtgcataaa caattccatg ttaggccaca catcggtaag ccggctcgca
     5221 ttgttgtact ccattatgcg gcggagcata attaagttcc aggagggtac tccggcgaaa
     5281 ctggttacat tggctttgct atatttgcgg catataatct ccactttctc atggaagtcg
     5341 tgcatcatcg ccgttttttt gtcgggaacc cggaaaaact ccgcgggagc aggagagttt
     5401 cgcaggagga ttgcagaaag atccccgctg aagctcttac cacccaaatc gggctccacg
     5461 ctgcctccaa gcgtaagcgc tttgcctgag tagatgcgtg agtcggggaa gcgctgtaca
     5521 taatttgcca gcatccgttg catccctccg taatgacacc cccagagatt ggtacgggta
     5581 atggggataa acttgctctt tcctgagctg gtacccgaac tttttgcaaa aaagcgggtt
     5641 ttttcattcc aaagtacata atcctcaccg gaacgcaatc gctcaatata gggatatagt
     5701 tcattatagt cacgcaccgg caccaaacgc tgaaaatccc tcaccgagcc tatctttgaa
     5761 aagccgtgat cacggccaaa agcggtatcc ttgcctccct tgagaagagc tcgaaacacc
     5821 ttcatctgca cctcaaaagg atgttttcgg gtacggtcaa gtcccttgag catcctgtgt
     5881 gcataggtaa gaaaagcttt gttgatgagt gacatatata aattatttat gacaaagtta
     5941 tgcaaaaata gtatgaagta ccgccaatga acggatgttg atgggcgtat cgagccaata
     6001 actgagatac tttagcattt gatcgcagaa gccgctcctt gccctcgcat tcagcgggat
     6061 ttgcattgcc tcgttgaggt ctgcattaag gagccggtaa agtagcaggg aatcttgctc
     6121 cgtaaagaga taagtcgagt cacgcaaggc cccggctgag acatatcggg cacttattat
     6181 gtcaaacagc ggagtctgct cagaatagtt gtcatgaggc aggaatccca tctgtctgca
     6241 aaatccaacc aaaaatttta ggtggaagtt tgcactgttt ccctcgaggg cttccagagc
     6301 ggatatcatt cctttgagcc attcaaatag ttcttcgtcc ccgctctcct ccctgagggt
     6361 gcggtatatc acttcactta caaaaagtgc aatggcactt ttgctgatgt cggtccgtat
     6421 ggaatggagc tggaccaggg gatggtattc ctgcatataa agcagcttgc ttttggttga
     6481 ggcagcggtg accacctcga gtatggcaag gttgtgaaat tgggcaatga cggcactgtt
     6541 tttgctcttt ttaatgccac gcagatagat gccacgccgt ccgagtctgt tgtcgagcac
     6601 gtgtgctatg agacccgaat cgccgtactt tgtcagatga agcactatcg tttgggattt
     6661 gcagacaagt gacataggag tttttccaac gctttgcctc tatgagagat ggcatttttc
     6721 tcttccagag agagttcggc catggtggca tccataccga gcggcctgaa gataggatca
     6781 tatccgaaac cgtcaaagcc atgcagagtg gtggtaatat gaccttcgca aataccttca
     6841 aaggtgtgca ggtggccatc ctctatcagc gcaatgacgc acctgaatct cgcggtacgc
     6901 ttttcgtaag gtacgccttc aagttctttg agcagttttc tgatattgtt ggggggagat
     6961 ttgggttcgc ccgcatagcg tgcggaatgt accccgggag cacctccgag agcatccact
     7021 tccagaccgg tgtcatcggc aaagcaattt ttctgaaatt tattccagat aaagagggct
     7081 ttttcaacgg cattttcgta tagagtgtcg tgggtttcgg gaagttctcc gtcaaatccc
     7141 agctccgagg gcatcacaag ttggaatctc ttacccagta ccctctgagc ttcggccagt
     7201 ttattggcat tgcccgtggc aaacactatt tccattattg atgattctga taattggcct
     7261 caaagatatg aacaaaatga taaaagtatt aattttgcag agttttaaaa cgagataaag
     7321 aagatgaaaa tttttaaaac cattgcggtg acgattgcga ttttggcatc agtgacctca
     7381 tttgcgcagt ccgccgaatt taaattagga cagaatatgg agattcagta ttcaatactg
     7441 aaagagctct ccaaatcata tgttgatacc atagaatatg gcaaaatcat acccatcggt
     7501 attcaggcca tgctccagtc tctcgatcct tacactacat acattcccga agaggaggga
     7561 gaggatttcg acatgatgac tacgggtgcc tacgggggca tcggttctct cataaaaaag
     7621 agtcctgaca gcggagtact gattgttgaa ccttattcgg gatccccggc catcaaggca
     7681 ggaattgtgc cgggtgatgt aatcatagct attgacggtg tcccggtcta tggagaaacc
     7741 tcagcacaga gtgcagctaa aatgaagggg cagccgggta cggaggtggt ttttaccatc
     7801 gtcagaggtc gtacgctcga cacggtcgac attaccgtta cccgtgagag aatacgcgtt
     7861 tccgatatag tctattcggg gataatcagg gattccatcg gttacatagc cctcaccgga
     7921 tttactgaga agatgcatct ccagttcaag gagagcgtac tcgatcttaa aaagagaggt
     7981 gctaaacgtt tggtaatcga cctcagaggc aatggaggag gcattatgga agaagctgtg
     8041 tcacttatgt ctctatttgt gccggaaggc actctggtac tctcttccag aggacgtgaa
     8101 gagaggatga atgaggagta ttacacaacc acttccccta ttgacacact aataccggtg
     8161 cttgtgatgg tggatagcgg ctcagcctct tctgcggaga tagtggcagg agctttccag
     8221 gacctggaca gaggggcaat agccggaagg aggaccttcg gcaagggact aattcagtcc
     8281 ataaaaccca ccctatatga tggtaaggtg aaaattacca ccggaaaata ttatactcca
     8341 agcggaaggt gcgtgcaggc tatcgactat agtcaccgca atgaagatgg tagcgtgggt
     8401 aatgtacctg atagcctcaa gcgtgaattt aagacacggc tgggtcgaag cgtctatgac
     8461 ggaggaggaa ttactcccga cttcatcact cagaccagat cttatagccg tccggcatat
     8521 tctctcgtct ataatgatat actgggtgat tatgcaatcg aatatttcaa gaagaatctt
     8581 tccatagttc ctgcctccga gtttcatctt accgatgagg agtatgaaga ttttatccgc
     8641 tatgcgggaa cccgcgaatt tgatatccgt agttctgctg aatcttcgct ggatcagctg
     8701 gtaaaggctg caaagcagga tggcctctat gatctctaca agagtgaaat cgatgctctc
     8761 tccgcaagga tcaaggtgga caaggagact atgctgcgta ttaagaaaga tgagatcctc
     8821 cctctgctgg aggaagaaat cgtagtcaaa tactatatgc gcggttcgga atatgagatc
     8881 cgtttgaggg atgatcaggg actttatcag gccctcgact ggtgggtgga atagttccga
     8941 gtgtgtagta tgtagtatgt agtaattggg tgttgcggtt acgtggtgcg aggtgtgtgt
     9001 tagttccgaa tattgagtaa gtttgagtgt gtcgcaggtt acgcggtgcg ggttacgtgg
     9061 taagtgtgta gtgagttggt gcgactttag aatattttta agaaaagaag aactactatg
     9121 agttatatta aacgtttcat gttgttgggg ctggtttttt tggcagtgct tagtgcctgt
     9181 caaaaggagg tctcttgcac tttgagcgaa tcgaatattg aattggctta taccggtact
     9241 cctctcaagg tgtacctttc cagcagtgtt ccgtggaaga tcgaagtgcc gaccgatgct
     9301 cagtggctgc agatcactcc tctttcggga ccggcagggg aagagataga actgactttg
     9361 aaagcgcagc cgaatgagaa gagggttcgc aaaacgcagc tgactgtccg ttgtggaagc
     9421 cgtattttag atctatccgt actccaacaa cctttaaatg ttgaggaata cacttatacg
     9481 gttttacaga ggcatacgca aggaacctat cccaacgaag tgattatctt gggcgatggc
     9541 tattccgcac tggattacgg ctcggagggc ccttttaata aggatgcgca cgatgccatg
     9601 gaggcctttt tttcggtaga acccttcagc acctatcgtt cctattttcg agtagctcaa
     9661 tttgtcgttt tttcaccgca aagcggtatt acggaacgcg ataaaaaaac cgaggtccgc
     9721 accgcttttg gtgtccgctt tgatgaacct tatgtcatga cgatggacga ccctaatttg
     9781 gtattcaaag cagctatgcg tattcccggt atgaccacgg agcgtatcag caatacatta
     9841 tttattcttc tggccaatgt tgaccggtat ggcggtctgt gttggcaata cggagacgga
     9901 tcggcaattg ccctttgtcc ggtttgcagg acatcggcgc atggaggcta tgcgctgttt
     9961 gccgatttga ttcaccacga ggccggaggc catggttttg ggcggctggt cgatgaatat
    10021 ataacacatc ccggagagac gctgccggac gaggcgacaa gcgcttcgcc tcaccgtcat
    10081 aaaaaggaac tgcaagggag gaaatatttc ggctatgctt tcaatgtaga tatcagcggt
    10141 aacaaagaac aggctccgtg ggcgcattat tatacgctgc cgggatacga ggcggtaggt
    10201 tattatgagg gggcctattt ttatgaaaag ggtgcctggc gaccggaaca gcgcagttgt
    10261 atggttgaca atcgtcctta ctataatgct ccgagccgtg aaagcatcgt taaattgttg
    10321 ttaagacggg cagcaggtgt caggttgtat gattatttgt atcaatcctc ttcagggact
    10381 tatgaagtgg tccctattcc gaacgatccg ttcaatatgg aggattttat gcgcaatgat
    10441 atgaagaaag cctggtcggc gccgatgcct ataccatccg atacccgaag cggccaatct
    10501 ttcatcccca cccaaccggt acgcttcata cccggctcac cgtttgacta actcaatact
    10561 ccttactcat aactaaaaac tgagggtcac caaccggtga ccctcttcag catcccctcg
    10621 gcgggagcca cggtttcggt ctgcctcttc ggcgaagcag acaccttaat tactagtcca
    10681 gaagctctac gattttggcc tcaggagcat gtttcataac ggatgcgata ccattgtctc
    10741 tcgtcctttc ggaagcatac atctggctat ttccaattac ctgaccgttg gtagctttaa
    10801 gattgaagaa gggcttgccg tttgcagcca ctttcttctc atatcttgcc tcttcaacgc
    10861 tgtttttctt tactgattca acgccgttta ggcaagcgga cttagtcttg tagccttcgc
    10921 tggtgaggat tatctcgcca tttgaagctt ttaggttgaa ttggaattct ccgttttttc
    10981 ttaaagtaat ttcaaatttt cccatatcaa tatagtgttg ttgtttagta cgtcaagtcc
    11041 tacaaagtta ggtaaaaaaa ttataaatca aaatgattta catatcttcg taaataaaaa
    11101 cagaccgaaa tgaaaagaaa atcatacata tttttctctc tgatactttg tgttttgtgt
    11161 atagcggaaa cgcaatcgtt atcagcacag actccatcgg gtgtgatggt aaaacatgtt
    11221 ccgactgaac ttatacaaaa tcccgataat ccgatattat taatgtttac tgcctcctgg
    11281 tgtgctccct gtcataggat gcgcagtgtg atgtttaaaa aggagaatat ttcgcttctg
    11341 ctcagaaaat acaatgtgat attgttggac gtggactcac aacctggctc ggatctgcag
    11401 agaatctatt gcgaagatag tgcggttccc caatttatag taatggataa agagcagaac
    11461 gtgctccgaa aaatgcgtgg tgctactgat agcgaatctg agtttgcgga gtttctgcgt
    11521 agcggtattg aagagataag acccggtctg ccggccgtgc aggatattat ttctcaggaa
    11581 gagatggaac tcctcaccgg tgatgatttc aaggcggcta cgagtcgcct caaggcgaag
    11641 tggagtttcg gggtgggtat cggaggccat atttccaata tgcctgtttc cagcagatct
    11701 ctctttgctg aagagagagc ctcggccctc gacagcacac aactcaatta ccgcggggtt
    11761 aagccgggcg ggagcatttc cgcattggtg cgttacacat tctacaaatc caccatagag
    11821 accggtctcc tctttatgag tgccggaggt cgtgatatac gtaccaaaca gagttttgac
    11881 tcatattata taggtgtccc ggtagatttt cattttggaa tttgggacaa tcttaagttt
    11941 ggtgcaggta tttcagccat gtatatggtg aaggatgttg caaaaaatcg ttttgatgcc
    12001 ggcttgcgcc taaaacttat ttgggatgcc tctccatttt gcataagtgc aggttggcaa
    12061 cgtggtcttg tcaatatttt gggggcggaa tcgccattaa gagctaagaa caatcacttt
    12121 tttgttaatt ttgcctttta tttcaattga aaccatcaat gagagtaagg catttattca
    12181 gatttttcat cgttacgacc ctctgggcct tcggttctat gctcttttct gcgacggcag
    12241 ctcctgaagg gagtttattt gcagatggtg aaaagagagt cgctgagttc acgcacgatg
    12301 aattgctctc gagaaatatg cctgccgaac tggcgtttct gccggaggct atatcaaatc
    12361 tctcaatgcc cggcaatcgt gcaggtatct gggctctcga tgccctggat gcggtgaggc
    12421 atggcctcaa ggtggatttg acacacgatc agcgctacga tgatacactc tgtacccggg
    12481 ccgccctgag ttgtttgcag gagtactatg accgttaccg ggagtgggat ttggctttag
    12541 ccgcctacct gcatagccct gctgtattga atgcatgcct tcaatccgga gattcgactc
    12601 tgctactcca tacgctgact ctctctgatc tcggtcgtat ggaaaagagc gatcattcct
    12661 ctcctgcgga ggaaatgtat accctgtatt tcgaccggcc gatctctctg gaagccgtga
    12721 cgcatcttac cggcataagt gctaaggagt tttccggtac aaatcccatt ttcagatcag
    12781 atcccctggt gcctctcgaa ggttacggac taaaatttcc tccctatgtg gctcaaacta
    12841 tagaggtgga ttccctttat gccgttacgg atcgaatggt ggcagccaga gaggccgaat
    12901 tgcttaaaaa acagaaagaa gccgaggagg agcgcaagca gacctccatc acttatacgg
    12961 taaaatatgg tgatactttg agcggaattg cacagaaaca cagagtcaga gtttccgata
    13021 tcaaaaagtg gaacaaactc aggagtgatt ttatacgaga aagacaaaaa ctaataattt
    13081 acaaataagg attgatgaaa gcgagcaaga tttttctctt catattgtcg gtaattttta
    13141 ttcttggagt ggtttgggct attttcccgc agggaggaat agaggttgga ggcaccactt
    13201 tgcgatttgc ttcctacgaa gaggccgctt ccggcataaa tgaggaggtg gtggatgtgg
    13261 ataaagtact ttccgatgta gagcagagtt tcagtatgct ctccggttct cagcaggata
    13321 cccttagata ttttaggaat ttcctcaaat acaaccctaa tcggatttgg cttccaaacg
    13381 acgactatac ctatcttgat gatctgtttc atctatttga aagcgcaaag aaggagaaca
    13441 agacttaccg gataatgcac tatggtgact ctcagataga gatggaccgt atctcttcta
    13501 tcattcgtga gcatcttcag ggtatcttcg gcggcaatgg taatggtctt gtgccggcaa
    13561 tccagagtgt tgccacctac tctttggtgc agagtgcatc cggagcactt acccgttata
    13621 cgctcatcgg tgactccact accgtgcgcg ccgatcacaa ccgttacgga gtaatgactc
    13681 agctctccca actctccggt gaggcctcca taactttcag ggtttccaaa aactccaatt
    13741 gtcaggagcg tgccaagaaa ttttctaaag ttgcattgct tgtaggcaat acttctcccg
    13801 gatttaaagc ctctttgcgc tgcgatacca tccgaacaca aaacaaagta ctcaaaaatg
    13861 cggtaaatgg agtttcgctc cttacatggg aacttcctgc gtatgcatcc agaggcacta
    13921 ttaccctgag tggcagtgga gagatatatg gtttcctaat ggatggcaaa aacggaattg
    13981 ctctggataa cgcacctctc agaggcagtt caggtaccat attcacccgt atgagcaaaa
    14041 gcgtgatgaa gcaatccttc gacctgctcg atacccgtat gattatattg cagttcggag
    14101 gcaacagcgc cgtcagcatc cgaaatgaaa agcaaatctc cgcctatttg cgtaaactcg
    14161 aaaatcagat tgactatttt cacgaagtgg ctcccgaggc gcaagtcctt tttattggtc
    14221 cttcggatat gggcaggagc gtagaagggc gcgttgccac tattcccatg ctgccggctc
    14281 taaacgattc gcttaaggta atggccctga gcaaaggcgc ggcatattgg gatctcttca
    14341 atatgatggg gggtgaaaat tctatggtgc gttgggtaaa gcatagtcct gcacttgcga
    14401 gcagcgatta tatccacttt acaaccaagg gtgccgagca tgtggggatg gcttttgcaa
    14461 gagcttttga agtatgttac aatttttaca aactcaggaa agagctggat cctgaaatgg
    14521 tatccgctta tatgagtagt caggattcca ccgaagtgcc ttttgctgac actaaggaga
    14581 gtgatataaa tatggtttca gaaggagaat tgggaagaga atgagaagag gcacgctagt
    14641 attcaggttc tttgttgcag caatcgtgct gtttgcggca tggagcggag atgcctttgc
    14701 tcagggtctc cacaaaccca ttcccaacta tatgttcgcc cattttgagg agaatgcgat
    14761 cactttcccc tcgggggata gtgcggattt cgagagattt tttctcaaac tcgataccct
    14821 tctctttttc ggagagggag atatcaatat agtccatatc ggcggttcac acgtacaatc
    14881 cggaactttt ccaaacagga tgcgccgaaa tttgctcgcg atgcgctatg gtatcgaggg
    14941 tggtagagga gtagtttttc cattctcggt agcgaagagc aacactcctt ccagttatta
    15001 cagcagttat accggtaagt ggaaagctta ccgcaactca cagcccaaaa cgcctctacg
    15061 tatgggtctg accgggatgg cggctgccac ttcagaccca tcggcaacaa taaccattgt
    15121 ctcacgtgag cgcaatccca tgggttctcc ccatgatttc agatttgaca gagtacaagt
    15181 actggggcgt gccgataacg attcgctcgt accggtagtt cgtctggata ccctgacgct
    15241 tttacccctc tctcacgatc cggatagaga cctttatgag tttgtgctgc cttacctgac
    15301 ggattctctg gtactgggta ccagcggagt ccccggagag tggaatttga cggggattgt
    15361 gctggaccat ggtcgtccgg ggctcaccta tcacggtatc ggggtcaatg gcgcgagcct
    15421 caactcatat tttaagtgtg aggattttga aagggatttg gctttgctca agcccgatct
    15481 gataatattt gccataggca tcaacgatgc aaacacctcc aatttcaaca aagagctatt
    15541 tatttccaat tatataaaaa tggtcgatct ggctcgtagg gtggcgcccg actgtgccat
    15601 actctttgtg accaacaacg acagtttcag gcgttcccgc cgctcatatt atctcaatca
    15661 gaacggccct gtggtacagg atgcctttta tagtctggcg cgtagatatg atgcaggcgt
    15721 atgggacctt ttcagaataa tgggcggtct caattctatg aagcagtggg agagtgccgg
    15781 actggccaac agggataaag ttcattttac ggaaaagggg tatattttga taggtgacct
    15841 gctttataat gctctgctgg atcgttatct ggagcatctc aaatcaaata cttcaaagaa
    15901 cggtaccaga tgagcggttt tatccatata gcggcagatt ttatcaaagg actcttcaca
    15961 tttgatcagt cccacccttt gctcttcacg caattctatt tctgggcatt ctttgcaatc
    16021 gtatttgcct ttttcgccct tttcaaaaat aagctgctgc tcagaaacgg tttcctcttc
    16081 ttcgccagcc tctttttcta ttataagacc agcggtatct ttactttgat actacttttt
    16141 gtgacgcttt acaatttctt gggcgccaaa tggatgcata ccagaaagag tgatggggga
    16201 aagagagccg tacttgtggt gagtcttatt gtcaacctct ttacgctatg ctacttcaaa
    16261 tatgcctact tcataaccga tgtggtcaac aaccttttcg ggacggagtt tgtcgtattt
    16321 gatttgtttg ctgtagttgg gaatgctatt gtgggtgcca atcgtttcag catcgatacc
    16381 ataattttgc ctgtgggtat ttctttctat acttttcagg cgatcagtta tataatggat
    16441 gtctaccgca gtagagtgac tcccgtgcgt aatattctgg acttcggttt ctatttgagt
    16501 ttcttcccgc aattggtggc cggtcccata gttcgtgcca gcgaattcat acctcagctg
    16561 caccgaaagt tttttctcag cagaagacag tttggcattg cggtattttg gattgtaaat
    16621 ggcctggtaa agaaaatagt ccttagtgac tacctggcca ttaatttcat agaccgtgtc
    16681 tttgaaaatc ctttgctcta ttccgggttt gaaaatgtaa tggccctctt tggatactca
    16741 ctacaggttt atgccgactt ttcgggatat accgatattg ccataggagt agcaatgctg
    16801 atgggtttct accttcctca aaacttcgat tcgccttata aggcaaagag tccggtagaa
    16861 ttttggcgaa gatggcacat gtccctatcc agatggctta aggattacct ctatatccct
    16921 ttaggaggta accgcaacgc taccttcggt acatattgct gcatcgtttc catttcactt
    16981 atcggagttt tactttcagg ttcgtggtgg gtagctctgg tcgtcgcggc cattgcagtg
    17041 gctctcattc ttattgcaag gcttaaaccc gcgaagcgca agagcatcac taccaatctc
    17101 aattcgatga acaccatgtt gctgggtggt ttgtggcatg gagcaagctg gaattttatg
    17161 atctggggag gcctgaacgg catcggaatg gtaatttaca aggtgtggca aaactgggga
    17221 ataggtttga ggtttcttgt cataacggcg gttactgtga cacttggtat cttatccgtt
    17281 gttgcgcccc gtccactata taatatgttt tttgtctgga tggcagtagt gatgacaggc
    17341 aatcttgtca aattgctata tgtgctgata tgtcgtattc gtggtattca ggcggctatt
    17401 gtggccgggc cggaagcctc taaagagtcc cgtttcagac gcctctcgga tatctgggcc
    17461 attttccaga catttgtctt catcaccttt acacgtctct ttttccgttc gggatccaat
    17521 ctcgatcccg cagaggccaa tgagacagcc tggaatacgg caaaaaatat ggtaaaccgc
    17581 attggcggcc attgggatat ggccatgata ccggagatgg tatggcaata tcgcaacgtg
    17641 tttcttcttg tggttatcgg tatggtgata cactggcttc ctgaccgctt taaacgccgt
    17701 tatcgtctct ggtttgcatc cataccgctg ccacttatgg ctctgatggt agctctggca
    17761 gtcttttttg tctaccagtt tataactgcg gacttgcagt catttattta tttccagttc
    17821 taaaaggtaa cgggatcgcc attctgattt ccgggatcct cgggatcttc gggtgcggcc
    17881 tcttggatca gtatcaaaaa acgttctatc gtttcactga atactcttgc tttagtctct
    17941 cgcctgggac cttcgttgag ggtggtctta aagataacag tggtgtcacc atcagcccag
    18001 ttgggtctga ctattaccca ttgattacta aaactgatat cgggtgtctc tatctcccag
    18061 atgacattgg actttatatc cagagaggca ctgtcaatat tgtaattaaa tatgatggta
    18121 tcccgtgaaa tggtcaattc cggaagaaca tatttttcac aggagacgaa ctgcgcgagc
    18181 attacaaaag cagtaacagt cgcaacagcc gaggccaaaa tggttttttt atctctcatt
    18241 tcgaagcaaa gttacaaata atccgatgtt gcgctctttt ttttgtcgat aggggaagat
    18301 atttttcatt ttttacttat atttgtcact tgatagatac agtatgcgta atattattcc
    18361 aattatagtt gcggtagcgc tgattgctac cgcttgtcac aaagtggtgg atccggagat
    18421 ttcagtagat ccttatgcca taacttgtga ctataatggt ggctcgttcg acgtcaatat
    18481 acgctgcaac gtggattgga cggcagattg cgataccctc gggataacta tatcgcccac
    18541 gtcaggaact aaagacggtt ccataaggat ttcagtccct tcaaccactt caaagtccac
    18601 tcaggtggcc agaataacat ttaccgcaaa gggtgaggag agttccaaga tagctaaaac
    18661 tgtcgttacc ataggctcct atccctttat cggggtagat gtaggtcagc tcagcattgc
    18721 agctgaggga ggcggcacgc aatttttact gacggccaac cgtcaatgga gtgccacttc
    18781 ttcaaatccc gcctttacct tttctccgga aaacggagcg gtaaatgata tcatcagggt
    18841 cactgccgac gtcaataata ccggctctcc gagggagtgt atcattaact tcaaacttaa
    18901 agaataccag aataaaacgg cacaaataaa aatagttcaa acatcactgt aataccatga
    18961 gaaaggcact gctaatcttt tttgcttcat tattcttttt tccggccttt gctcagaaat
    19021 atcaattttc gggtaaggtg cttgaaaaag gtaccgatgt ccctgttgag tttgccactg
    19081 tagtaatggc agataaggag ttgtgggccg tagccaatgc aaaaggagaa tttgtaatca
    19141 acggagtttc cagaggtaaa actaaagtag agatatcctg tctgggatat gctccaacca
    19201 cgtttgaaat agaaataacc aaggacctca agggagttac atactatctg tcggtagatg
    19261 acctgaccct tgagagtgcg gtggttactg caaaagagaa tacaaacagc gctaccacca
    19321 gccgtcaaat tgacaggact gcactggagc atatgcagat ggttaacatt tcggatatct
    19381 cctcactgct gccgggtgga gtctcaacct ctaccggtaa actcaccaac tcaaccgagg
    19441 ccttgcaaat tcgttcagac gagacctcgg cagtcgcctc tttcggtact gctgttgaac
    19501 tggatggtgt ccgactctca aacaacgctg cctacagttc ggtatcagga gtctccaccc
    19561 gtaatattgc ctcgtccaac gtagagtcca tagagatcat aaccggagta ccatctgtgg
    19621 agcatggtga tatgactaac ggaattgtca agatcaatac ccgtaaagga aaatctccct
    19681 ggatgataac cgcaatgacc agtcccaaca ccaaacaact ctctttaagc aaaggctttg
    19741 accttgggtc gaaaggtgga gtaatcaatg cgagtgccga gaggaccact tccgtacagg
    19801 atctggcatc tccctacact acctaccagc gtaacgcctt ttcattgctc tattccaatt
    19861 cttttgggac gttgggacaa ccgttaagat tttcattcgg tctttcaggg aatgtcggcg
    19921 gatatgatag gagcgctgac ccggattcct tcatgaatac ttactcaaag tcgaaggaga
    19981 ataccatcag gagtaatttt tcactcaact ggcttctcaa caagtcctgg attaccaata
    20041 ttgagttgac gggttcgcta gtttattccg ataatttcag ctcctatagc gacaacagat
    20101 ccagctccag ttcgacctcc tccctgcacg gaatggaaga gggatacttt gtttccactc
    20161 cctattcatc caatcccaat gcggaggtta ttctcagacc ggccggatac tggtatgagg
    20221 taagtcacgg ggataataag cccctcgatt acaatctaca cttaaagata aattgggctc
    20281 acgacttcgg aggcattgtc aacaaattaa aacttggact ccatttcacc ggaagcggta
    20341 atcttggaga gggcttctat tatgaagatc tgtcgaaggc acccgattgg agaccctatc
    20401 cctataagga tattcccttt gtaaataacc ttgcggccta tggcgaagaa aatataaata
    20461 ttcccattgg caacactacc ctcaatcttg tagggggtgt acgctcggaa tggactatga
    20521 tcaacaattc aggttatggc acggtcaata gcctctcacc tcgttttaat gcgaaatact
    20581 ctatcatcaa atatcgcaga agccgcatat tgagagaact ttcggttcgt ggcagctggg
    20641 gtgtggccgt taagctccct tcggtctcaa ttctgtatcc gacaccatcc tactacggga
    20701 aacaggtatt tgccccgggt acgactgcgg atggctcttc attctatgcc tattacattc
    20761 ttcccagaca gatagagtac aatcccgatc tgaggtggca gaaggcacaa cagagtgaag
    20821 tgggagttga gatggatata gccggcatca aattctccct ggcggctttt tattcaaaaa
    20881 ccctggactc ctattttact tacagcgagt atgagccctt tacttacaaa tggaccgacc
    20941 agcgcaacct tgaaaagaag tgcccgatcc cctcttccga tcgtctctac tccattgaca
    21001 gggagaccgg agttgtcacc gtaaaagaca tcacaggtaa attaccggat cagacactcg
    21061 aatatacgga gaaaaatacc ttcgtatcaa agaccaaagt taccaatgag gtctcaccga
    21121 ccatacgcca aggtatcgag tgggtgatag attttggaca gatacaggca cttcgcacct
    21181 ccatcaggat ggacggtaca ctatataaat ataggagtgt taatgaaaac atagtgcagt
    21241 cctataattc cagtcgtaca atggctgatg gtataacgcc ataccaattc ataggatact
    21301 atgtcggcag cagttccggt accagcaacg gttccgagag tgtgaagctc cgtaacaacg
    21361 tcactcttac tacccatatt ccccaggcac ggttgatatt tactctcaga cttgagagtt
    21421 cgctctaccg ctatgaccgc aatcttgcag aatatagtgg cggacagatc gcctggccaa
    21481 tgagcggtaa cgagaagatt ccctccggta ccgagtttta cgacattcgg gggcatgttg
    21541 tcaaatatcc cctctattac tctacatttg aagagcccaa tatcctgata ccattccttg
    21601 aggaatatct ttgggctaaa gagaacaata agaagctctt ctctgaattg gatgcaatga
    21661 tcaacaaaac gggttacact catctattcc ttaagaatgt cttttctccc tattttgcag
    21721 ccaacataag tgtgactaag gagataggta agatagcttc cctttccttc tatgccaaca
    21781 atttcttcta ctcgacaatg aggttcaaaa tgagcgcttc ggggaccgat gcatcattat
    21841 ttgaatccac ctcctatata cccaagtttt actacggact cacattgagg cttaaaattc
    21901 aataatataa acacactatg cttatgaaaa aaattgtttc attcctgatg ttcagcgtga
    21961 tatttctcgc tgcggtctcc tgtcacaagg acgaccctta caaattgtat gacgtcaaag
    22021 ttcagctggt ctatgctcaa ggtagtgact tcggtgttgt tgccgacgtt acggtaagtg
    22081 cagtaaacac aaataccgga atgatttatg tcggtacaac taatagcgaa ggccttgcga
    22141 tcgtccccgc agttgccggt ctatatgaaa tttcggctac tgagacacgt gccgaaggtg
    22201 gtaccgccta ttccttcaat ggttttaaac agcaggttgc cattacccac gattatgaga
    22261 aaaatccttt tgtagttacc atcgacatgg taggttcaga ggctaaacag gttatcatca
    22321 aggaacttta tacaggtggt tgtcaaaaag atgacggctc cggtaatttc caaatggata
    22381 aatatgtagt cctctacaac aactcgccgg tggatgccga cctcaaaaat ctctgcttcg
    22441 caatgacaat ccaatacaac tcacatacaa cgaatgcaga cgtggttgat ggaaaactta
    22501 cttacgaggc tcaaggtacc gtccctgccg gtaacggttt ctggacatgg gctgaaggtc
    22561 aggctcccaa actgaaaccg ggtgaggagg ttgttgtggc tataaatggt gccataaacc
    22621 acaccattac ctacagtaag tctgtggatc tctccaagag cgcctactat gcgatgtttg
    22681 accctgagtc gggatacaat catgcatcat actatccgac accatccgag gcaataccta
    22741 catcccatta tctcaaggcc tacaagtatg gcgcaggtac gggttggcct atttcggcga
    22801 caggccccgc tttctttgta ttcatcactc cggatggggt aacccctcag tcattctttg
    22861 aggatacttc caatgagaat ttctacaaca atttgagtgc aatgaaacgt aaatgggtcc
    22921 ctgctgactg ggttgtagat gcagcagaag tcttctcagc cggctatgtt ggcaagaaca
    22981 acaagcgtct catcactgca gttgacggag gtagtgtagt actcaccagt aagatgggat
    23041 attccatcta ccgtaatgta aacaaggatg ccactgaggc catacccgga aataaaggga
    23101 aaattgtcta caattacagt atgggaacgt ctgatcttga agagggcacg accgacccga
    23161 gcggtatcaa tgctgaagcc tcaatcaagg ccggtgcgca tattatcttc atggatacca
    23221 acaactccac aaacgacttc caccaacgca aagaagctgc tttgagaagc aaataacaca
    23281 aagtgataat gaaacgctat ctgactatag tgacaatggt gctgctctgc ctgatttcag
    23341 gtcgggcaca ggtgactccc aagcattttt atgattttga tttcataaag ggttcggagc
    23401 cctggctgac cagtagtaat gcatccggtc ttattcgtct gcctgtggag aagatcttca
    23461 taacgcaggc aggatttgac aagaataatg gcggatttgt ggcccatcac cagtcagata
    23521 acagtttcaa cgccggagct ttggcggaat cattctccca tttgggacag gacgtctatg
    23581 tttatgggag attgtcgtat gactatttta aagggaaaaa tatgggtggc tcagtaatga
    23641 gcgacccata tcattcccct ttcaatctgg tggcaatggc cgacagcact gccggagtca
    23701 agacactgga agtttataat ctggccggtg gagtcgctta ccgtttcaat gatgctctgg
    23761 ctgccggagg caagataaac tacacaagcg gtaactattc caaggcacgc gacccgcgtc
    23821 acgtaaatat gataatggat atggaagtaa cggcgggcct gacctggaat attggaaaca
    23881 aagtcatcat tggagccgat tacattttcc gaagaaatct cgaaagtttt tattatcagg
    23941 tttacggcac aaccgataag gtctactatt atttggtgga ttacggcgga ttttttggtc
    24001 agcgggagct ctttggagag aggggctata cgacccaaac cactaccgta cctctgctta
    24061 ctacctacca tggaggagct cttcagctgg agatggcatt ttcgcccggg gtaaagttgt
    24121 tcaacgaatt ttcattcaag gcgtttgacg gctattatgg aaagaagtcc gtaactacgg
    24181 taatgtattc agctcataat ggctaccagg caggctataa cggccatttt tccttcggaa
    24241 aggacaataa ccgtcatata gtgaaactca ctgccgacta caccaagatg atcaacaatg
    24301 agaatgtctt tcaggaaaaa actactgagg gagttaagga gatcatttat ttcggcagca
    24361 atgaggctct ggacagagat ctgattgaag gcaatttgga gtattccctc aatctcggca
    24421 tcagggatca tcgtcctaaa ctggtgctga aagcctctgc agatgcgctg tatcgcactc
    24481 agacagcttc tctatatcct tactaccgca agcaggattt attcgccatt gacgggctgc
    24541 tctcaggagc gtacaatatt ttccgtgaaa acggtataat gggaattcgc ctcggagcag
    24601 gatttggggg tggctccggc tctcctttca atgacggact ctacatgccg accaacaacc
    24661 caagaactcc gcgcacgatg gacaacgcga tgcggatgga gtttgaatat ctcactgcat
    24721 accgcatcaa tgccggtgcg ggtttcagat actcccattt tttcaaaaat aatattaccg
    24781 gctatgtcga tttggactac cggtttgtaa atgccctcgg catctcatat cttgccggtg
    24841 ctatgcatca tcgcatcagc gctatagtag gcatctcatt ctaataccaa acaaaatgat
    24901 taaaaaaccg atacttgcgg tgatggtcct ctttctcgcc gccgtacttt gccgtgccga
    24961 tgagggcatg tggcttatta acctaataga gcagggactc gccaagaaga tgcaggaaag
    25021 gggcctacag ctcgatccga acactctcta tgacgaagag aagggagggc tgagtgatgc
    25081 tgtcgtgtcg ctcgcttttg gctgtagcgg cagcatgatc tcatcggaag gtctgatgat
    25141 aaccaatcac cactgcgcct attcggacgt acacgcgttg agtacccctg aaaaaaacta
    25201 tcttgaagat ggattctggg ctatggagcg ctcacaggag attccggtca aaaaagacgg
    25261 tatctggttt cttaagaaag ttatagatgt aactgccgaa gtacaacatc tgcgtgactc
    25321 aatagttccc agaggcgggg gatttgcaat gcgaagggtt tacagtttaa tagagggtaa
    25381 atatagcaag atatttgaag ggaagggaga ggtctcctgt gcctcaatgt ggggtggcaa
    25441 caaatattat atggctctct atgaggtcta caaagatgtg cgtttggtag cagctccccc
    25501 tatctgcata gcatcctacg ggggtgaggt agataactgg gaatggcccc agcataaggg
    25561 agattttgcg ctataccgca tctacacggc accggatgga tctccggctg attactctcc
    25621 ggacaatatt cccatgaagc ctacccgtac gttgaaaata tccactgccg gtgtgcagag
    25681 aggagatttt gccatggtca tcggctaccc gggcagcact gacagatatg catcctcatt
    25741 taaggtcaac aacacggcaa ctgttgtaaa tcccatacag gtaaggatcc agggtgagca
    25801 gatgaagata attgacggtt ggatgaatct tgatccggca gtgcgtctga aatatgccga
    25861 ccactacttc tcattgagca atgtgcagga gatccgtgaa ggtgagatat tctgctatac
    25921 caagtatgat gtagtgggaa aaaaacggga acaggaggta gagcttcacc gctggataat
    25981 gtcggattcg ctcagagctc aacgttatgg tactttgctt acggatcttc aaatcaagta
    26041 caagaccctg gaatatatag agacgcagat cggctatttt cgtgagacaa tggccagagg
    26101 agtgcgtttg caccgtatga tcaacaatgt cgccagggct gcggaattat tggcaaagag
    26161 aaaaggctac caagtgccaa tagccgatag tacagctgct cttaaggcaa taatggattc
    26221 ctacggagag tttgacatga gagtagaaaa gg
//
LOCUS       DAOSIE010000003        23784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_2372_length_23784_cov_4.380294, whole genome shotgun sequence.
ACCESSION   DAOSIE010000003 DAOSIE010000000
VERSION     DAOSIE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23784
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2372_length_23784_cov_4.380294"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLI69_00270"
     CDS             <1..219
                     /locus_tag="PLI69_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40957.1"
                     /translation="MEQTSNEMFAGLKDKEIDPNLMERLRELFTTADFVKFAKHTPSI
                     GENEEAIPTAVRFVNATFLQEIEKEKEE"
     gene            223..1209
                     /locus_tag="PLI69_00275"
     CDS             223..1209
                     /locus_tag="PLI69_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG40958.1"
                     /translation="MFFEYPYLLWLELILIPLIALYIWREFRGSSPSMIVSNATPWKV
                     KGGTFKKAVRHLPFILRMIAIAAIIVAIARPRTSETFETVDTEGVDIVLTMDISTSML
                     ARDFKPDRIGAAKDIAIEFVASRPSDRIGIVVFAGESFTQSPLTTDRVTLINLMKEIE
                     TGLIEDGTAIGNGLATAVARLKDSDAKSKIVILLTDGVNNRGEIAPLMAAEIAKTYNI
                     RVYTIGVGAMGTAPYPVMTPWGVRLQDIEVEIDEKLLQEIATQTGGKYFRATDNTKLL
                     EIYGEINAMEKSKTTVDSFPIYSELFMKYASIALLALLVELILRLFVIRRIP"
     gene            1211..2224
                     /locus_tag="PLI69_00280"
     CDS             1211..2224
                     /locus_tag="PLI69_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG40959.1"
                     /translation="MIHLAQGEYLLLLLIIPFLFIFYGVSLRIKKRRIAKIGDTALVS
                     KLMPDVSRGRGWLKITLLAIAWFFFVLGLVRPQLGARLKERQSKGVEVMVALDVSNSM
                     LAEDYSPNRLERSKLAIERLVDRLHGDRIGLILFAGQSFVQLPITTDYVSAKIFLNSI
                     NTGSVPIQGTALADAIATSARSFSTQSENSRAIIIISDGEDHEGEVMDVARSVAESGI
                     RIYCIGVGSPEGKPIPIKGELLKDNYGEIVVTRLDENTLRQIAGIGNGLYVRAGNSEF
                     GLNPIIDHIKEMDEQEFQSVVFEDFDEQYMYLFGIALFFLILELLIPGKKGRRQLFKM
                     EDK"
     gene            2221..3033
                     /locus_tag="PLI69_00285"
     CDS             2221..3033
                     /locus_tag="PLI69_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG40960.1"
                     /translation="MMKRIILILFAFSLVVPTALAQSDRSQVRRGNRDFRKEKFQEAE
                     ISYKKALVADSLSNAAGYNLGNTFFRMENFTEADKYYSSVADSLGKGKRGSDIYHNMG
                     NSLLSQKKFAEAIEAFKNSLRRNPGDMETKSNLAYAQKMLQEQQQNQDQNQDQNQDQN
                     QDQNQDQNQDQNKDQDQNQDQNKDRDKKDNKDQDKDKNQDQDQNQDQDQNKDNNDNKQ
                     DQSQGQTPPPKITPQAAQQMLQAIQEKEKETQEKVKKEKALLLKSKQKEKNW"
     gene            3053..5653
                     /locus_tag="PLI69_00290"
     CDS             3053..5653
                     /locus_tag="PLI69_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOG40961.1"
                     /translation="MKRTLTFLFILLVSAKATFGQGLTVEAPGVVAMDETFRLVFTSD
                     GKMSDFNWPGSEDFNVVWGPQKGTSSSVSIINGKRTSTHNETVTYLLQAKGEGRFTIP
                     VATATIDKQQYSTRSVIIEVVGSQQERQSQSQSQQTNTQQSQSDSRVQSASSDDIFLR
                     LTLNKRNVVKGEPIIATLKLYTKVDIASFEDVKFPTFNGFWSKEVDTPQNIEFNRENV
                     DGTIYNAALLRRYMLIPQQTGKIKIESAEMVCVVRIRAGTTGPRSIFDDFFDNFQTVR
                     KRLSSPETTITVNALPTAPASFGGGVGDFKISTSLSKDSLTAHEAASLTVTISGTGNI
                     SMLEAPKVEFPSDFELYDVKTSEKVSYDGTSGSKTFEYPFIPRSHGDFEIAPIEYSFY
                     DLSGKRYKTLNTGTLSVKVGRGSGIDNGGVVMPGVNRQSVKNLAEDIRYISTGAPLLR
                     DKGRFFAGSLHFYGLIALIVLLFFAVGAILKKRAARRMDVIGTRNRRAKKMARARLQQ
                     AGSYLKQGLSGAYYEELHKAMLGYVSDKLIIPVADLSKERISSSLAEKNLPEQLITQF
                     ISILDRCEFARYAPEVKTEEMESLYDESVSVLSEIESKLKSVPKRPRGNVASIVALLL
                     LVTPFAVSAQESVDDLWAMAADYYAEGDYVNALNFYLMIEGEQKVSPDLYYNIANSYF
                     KSGDNSHAILYYEKTLKLDPSHADAAHNLAIAQRFTLDKIDILPELILVTWVRKVKYL
                     FSADVWAWIAVALTFAVAMLMLGFRFSSSLRRRKTSLVLACVVFLFAIISFAFSISEK
                     NDAIRDESAIVMSPVASVKSSPGEAGNSIFVLHEGTKVNLLDELGEWTRIELSDGRQG
                     WIAAGSIIKI"
     gene            5713..6327
                     /locus_tag="PLI69_00295"
     CDS             5713..6327
                     /locus_tag="PLI69_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40962.1"
                     /translation="MPLRAQYYFAIEDGSQASFRVYDNKGDVLGYYDETLKIVSAESL
                     DDIHAVLEQVYFDKDGKHLFKDKSSNLSLHIKEGEVTAKLETIKQGMTTKDLMVRGCV
                     IYVPSDIKSGDILPDRTMSIKVGIIGGTIYLTERKVLGMESVTVPAGTFNAAKVREKQ
                     KTKVAIISEEVTIISWMVKDLGCVRQESYDSKGRLVQIIEMVKK"
     gene            complement(6317..8230)
                     /locus_tag="PLI69_00300"
     CDS             complement(6317..8230)
                     /locus_tag="PLI69_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOG40963.1"
                     /translation="MKRILFITALCLSALMLSAQTERVKFAFIADTHIAEGSGTNDDL
                     LQCINDINNLKKELQFVIFAGDITEFGSDEEIFLAKKIIDNLEIPYYIVAGNHDSKWS
                     ESGCNTFAKVFGYENFEFEAGGIKFLGSNSGPNMRMAPALMPHESLVWLDSIAKVTPL
                     KQPVIFVNHYPQDTSMLNYFQVLNTLKQTNIQLVMGGHWHRNTILDYDGVPGVLGRAP
                     AAGRNGNVGYNIVNIENNRISIYERQAADNNGKGGKSGKAWYSREIFSEPQFTPDKAS
                     GRNNEYALPANFPWLTFDVNRKYPQVKAIWKIQDESDIGSGAVKAGKYILYANTQGVI
                     KALDSATGSLRWRFATGGKVFSTPAISGSRVVIGSTDGYIYCLNLRKGTLLWKYKCKK
                     SVLGSPVIMDNVVYIGASDNSFKAISLRKGKLIWQYDSVKGFVECKPFADKEQIVFGD
                     WANSLYSLDPATGKEQWVWRNKGSRMLSPAAVWPVKSNGKIFIVTPARKCHAIDAGTG
                     KEVWSADGGRESIAISKDGSKIFVKNMFNSIQCWSTLSDDARLEWNIQSDLGYDIAPT
                     PAATILLKDGREMLIVPTDKGNIFCFDGKNGNLVWKHKISVALINSIEPIGQDRLLIS
                     TMDGVVTMLSVTF"
     gene            complement(8235..8819)
                     /locus_tag="PLI69_00305"
     CDS             complement(8235..8819)
                     /locus_tag="PLI69_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOG40964.1"
                     /translation="MGNEKQIVLGITGASGVIYAERYIRGCANVQEISLSVILTGTAR
                     NIWLRELRYSPEEMISECGATLIPNDNFNAPFCSGSAAADTLLLLPCSMGALARIAGG
                     LASDAISRIADVQLKERKELIIVPRETPLNLIHIHNMKLLTEAGAVICPASPSFYHNP
                     VTIDDLVDSFVGKLLHMTGVKPLEKRYQWNNNQE"
     gene            complement(8812..9498)
                     /locus_tag="PLI69_00310"
     CDS             complement(8812..9498)
                     /locus_tag="PLI69_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOG40965.1"
                     /translation="MKKIVFLTGAGISAESGIKTFRDSDGLWENYRVEDVASIEGWYS
                     NPKLVIEFYNERRRQLETVKPNRAHEVIAELEKDFDVTVVTQNVDNLHERAGSTNIIH
                     LHGELTKIRPVDGDDGDAIDIGYADLSINDKDSRGVQLRPHIVWFGEAVPMIEKAARI
                     VSKADILVIVGTSLAVYPAAGLIHYAPSGIPLYLIDPKPSGLNHPRLTQFVEKATTGM
                     ERLKNELING"
     gene            complement(9508..10590)
                     /locus_tag="PLI69_00315"
     CDS             complement(9508..10590)
                     /locus_tag="PLI69_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40966.1"
                     /translation="MIITFVLQMNLPFNYDRIAENHDIIGRRAEAELFFKSIYDRKRS
                     VAIYGAPKSGKESFIRYSLDTLESRGLSFILCEINLFNIRTMEDFISLFKEKMLECYN
                     EIHYNSILPFEIHVDSLDERKILNLPEIIATDAGKLIIIYFKEFQNLLQCSNDQIDLE
                     DLDKIWSKQTNVRYIFSGSFVNQMKYIFEEKKYFYFMCDVISLEKLKKKECVDYIVRS
                     FQTLGRVIQDEQAGTIYHLADGNIWYIKHLCSICMSMPIGYVNNNIIKKATDSLISLH
                     TPRFMQIMMDLTPNQINFLKAVVDGVQRFSSQEILEGYHLNSSANVFRLKDALKKKEV
                     LTFDKEDNAIILDTLFAYWLKNTYFQ"
     gene            10783..11091
                     /gene="rplU"
                     /locus_tag="PLI69_00320"
     CDS             10783..11091
                     /gene="rplU"
                     /locus_tag="PLI69_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOG40967.1"
                     /translation="MYAIVDIAGQQFKVEAGKKIFVHRLAAEVGASLDFDKVLLTDND
                     GVVKVGAPYVEGAVVKATVLDHLKGDKVIIFKKKRRKGYQKKNGHRQYLTQIQIETIA
                     "
     gene            11110..11367
                     /gene="rpmA"
                     /locus_tag="PLI69_00325"
     CDS             11110..11367
                     /gene="rpmA"
                     /locus_tag="PLI69_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOG40968.1"
                     /translation="MAHKKGVGSSKNGRESHSKRLGVKLFGGQSARAGNILVRQRGTV
                     HNPGENVGMGKDHTLFALIDGTVVFKKKANNKSYVSVLPQA"
     gene            11449..12723
                     /gene="serS"
                     /locus_tag="PLI69_00330"
     CDS             11449..12723
                     /gene="serS"
                     /locus_tag="PLI69_00330"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOG40969.1"
                     /translation="MLTLKLLREQPEFVIARLAVKHFDAKEIVYDIIDLDKKRRSAQQ
                     ELDSCLAEQKIKASEIGKLMKEGRRDEAEGVKSQVASLKERSRTLEEEMAAAQQEQDR
                     KLVLLPNLPAAQVPEGRTAEDNVIVRQGGVIPDLGENAKPHWELVKELDIIDFDLGVK
                     LTGAGFPLYKGAGAVMQRALMAFFLERNSSAGYIEYQPPYIVNEDSGFGTGQLPDKEG
                     QMYHIAVDGFYLIPTAEVPLTNIYRDVILQDSDFPVKMTGYTPCWRREAGSYGKDVRG
                     LNRLHQFDKVEIVRIERPENSYAALDEMVRHVESLVQALALPYRIVRLCGGDMGFTSA
                     ITYDFEVWSAAQQRWLEVSSVSNFESYQANRLKLRYKDEEGKSQLAHTLNGSALALPR
                     ILASIIENNQTPDGRIVVPEVLRKYTGFEIIG"
     gene            12756..13292
                     /gene="ruvC"
                     /locus_tag="PLI69_00335"
     CDS             12756..13292
                     /gene="ruvC"
                     /locus_tag="PLI69_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOG40970.1"
                     /translation="MGVDPGTSLLGYGVILVDKKKVHYVDMGVIDLRKEKDHFLKLRR
                     IFKEIGELMDKYAPDDLAIEAPFYGKNPQVMLKLGRAQGSAIAAALHRGIPVSEYAPR
                     KVKMAITGRGDASKEQVRVLMEKTLGIKMESAFLDASDALAIAMCHFYQLISPLVDVS
                     ASSNWKSFVESHPTRVKK"
     gene            13382..16297
                     /locus_tag="PLI69_00340"
     CDS             13382..16297
                     /locus_tag="PLI69_00340"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG40971.1"
                     /translation="MRQKVLKAVALILLIFPTLVSAQRGGFTVKVTLTEAASGAGVEF
                     ATVSLTEAGSDKAYKYALTNENGFVEIRGVRAGKYLIRGELMGYVPYRDTINVDKNLD
                     LGKKKMDVEVHFLEGATVTDLGNPIVIKKDTIEHNIGLMKVTDNETLEDILKRLPGIE
                     VSADGTITANGKEIKKITIDGRTFFLDDPQLASKNIPAKIIEKVKVVDKQSEQAEFTG
                     IDDGEEETIIDLSVMKGMMNGWFGNIMAGGGSDLRGKDEDGVAIDNDFRFQGASMIAK
                     FSESDHIAFLGNANNTNNRGFNDLAGNLMAGMRGGGMRGGGMRGGGGGMPGMWGGRSG
                     ISTSYMAGLNAGKTFDNKSELTGNYMFNGNERVVEESTAKTTFKQDGSSLFAIDDGYS
                     ITNSYGHRAGARADWKISEQTSILFQPQFNFGTGNFEERSDFATDNISASGQKSKIND
                     GYSLSTGENSNQTARGFLLFRQRLGRPGRTISLNIDYDLSNNQTDGINRSLTNSYKNN
                     ILADSTLVDQFYHQSAESYGAGARLSYTEPLGKNFFAEATYSYDYSYRNSYKTTYDDY
                     ETGAIINHEYTNDITNESMLQRAGINIMKQEEKYNITIGATAQPSRTINVTEVGGNST
                     TIDRKVVNWAPNARVDFNFSDYEMLRFRYRGRTTQPSITQLQTVPDNSNPQRVTLGNP
                     YLDPSFTHRLNMMYNKNNVQTFSSLMLNADFNYNQRNIVNASWYDKKGVQYTIPMNND
                     KGTFSTMARLTYNSPIAKSKFSVMSFFNVSYSTGVSFVGKDELLDIDNPSTYLNVNNF
                     EENEYQNLSFSENLRFVYRDDIFEVSVGGNVRYSQAWYTITAENVRPTWTNTVNANVI
                     ANIPNIFNFSTDAAYTFYYGYEEGYNEPSLVWNAELSRQILKNLTISIKCYDILNQSR
                     NTFRTTTDNYVQDTRNNTLGRYLMVNLTYRFGTFGNQSLGGRMGGHGRGGGPGGMYH"
     gene            complement(16298..17359)
                     /locus_tag="PLI69_00345"
     CDS             complement(16298..17359)
                     /locus_tag="PLI69_00345"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG40972.1"
                     /translation="MTDLQSDPDLLQDEEQELFEHYRFEAEKGLTPLRVDKFITNRME
                     QTSRHRIQLAINAGYVLVNDRIVKANYKVKPLDVVTIMMPYQRRGFEVLPEPIPLNII
                     YEDEDLLIINKPPGLVVHPGRGHASGTLVNALAYHLGISPGIDQEDERMGVLVHRLDK
                     ETSGLLVVAKSEDAQFHLTKQFFDRTVERKYVALVWGNIQADSGTIEGNIGRDPNDRL
                     RFKVFSDDEQGKPSITHYQVIERFGYVTMVECILETGRTHQIRVHMNHIGHPLFNDER
                     YGGDRILKGTIYTKYKQFIDNCFTLLPRQALHAKSLGFIHPRTGEEMRFESPIPSDMT
                     ALIEKWRKYAGVRELEEEE"
     gene            complement(17352..18140)
                     /locus_tag="PLI69_00350"
     CDS             complement(17352..18140)
                     /locus_tag="PLI69_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOG40973.1"
                     /translation="MKTNWIVRNILLAVAIFAALIIIIFGSLNIITRHNRELSVPDFT
                     NMSLEDAHNLAEKRHVRIEVTDSVFVKRLDRGYIFSQNPVAGSKVKKGRRILITINAI
                     NPQMVEMPSLVGFSLRQAKTELTSKGLSLGSLIYVEDIATNNVLEQRYEGKPIAPNTL
                     LESESEIDLLLGLNPEDSQTYIPHLTGYTYTVAKDYLIDNSLNVGLVRFDETVINYTD
                     SLEAVVYSQSPAFSESLPFVCGTKVSIYLTKDQSKLAIKRNYND"
     gene            complement(18150..19202)
                     /locus_tag="PLI69_00355"
     CDS             complement(18150..19202)
                     /locus_tag="PLI69_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOG40974.1"
                     /translation="MKRNSNRYGFDQLMDLYSGDMFRLRSLAFAEREARYGKTIFFNR
                     NFHIEPSNVCIHQCSFCSYRRDSEDQPGAWSMDLDQIRDYCLQKYSKGITEVHIVGSV
                     HPERDFDYYRSIISLVRELLPNEVAIKAYSAVEIEDMSISSGLNLEQVLIQLKEAGLD
                     ALPGGGAEIFDSHIRNQICPDKLSAERYLEVHRTAHKLGISSNCTMLFGHIEKREHRI
                     AHLLALRELQDDTGGFSAFIPLKYSCANNSLSGIGEVSEEEVLRTIAISRLALDNIPH
                     IKAYWPMLGKRTSLLAMEYGADDMDGTIEDSTKIYSMAGSEEQRPVLAVEELGELASA
                     SGFQIQERDSFYRIIS"
     gene            complement(19275..19427)
                     /gene="rpmH"
                     /locus_tag="PLI69_00360"
     CDS             complement(19275..19427)
                     /gene="rpmH"
                     /locus_tag="PLI69_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOG40975.1"
                     /translation="MKRTFQPSQRKRRNKHGFRERMSTPNGRRVLAARRRRGRKKLSV
                     SSEELW"
     gene            complement(19496..20113)
                     /gene="rsmG"
                     /locus_tag="PLI69_00365"
     CDS             complement(19496..20113)
                     /gene="rsmG"
                     /locus_tag="PLI69_00365"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOG40976.1"
                     /translation="MENILRYFPDLTPLQRDRFAALEHLYVEWNSKINVISRKDIGSF
                     MTHHVLHSLAIGKAMSFAPDESLLDLGTGGGFPGIPLAILFPETKFTLCDSIGKKIRV
                     AESVSQSLGLENVICLNSRAENIGESFDYVVSRAVAPLRELYPWVKDSYRKAIISLKG
                     GDLQEEIREFQRGWRVKNERITIIDITSFFEEEYFTEKKIIIISR"
     gene            complement(20118..20684)
                     /locus_tag="PLI69_00370"
     CDS             complement(20118..20684)
                     /locus_tag="PLI69_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG40977.1"
                     /translation="MAKIGSKHSNSDDYNLVIAARNGDQKAFSALYDKYRSALLAFLL
                     KYVSITEDAEDICQRSFEKAFMNIGRYNPQYAFSTWLYIIAQNEAIDHIRKARNALKS
                     VSLSGGEADLRDISVTYSPEEMVIIKQDMDLFTTRILNLPEKYREVAKLRFLSDYAYE
                     EIAKELQITLGAVKTRINRAKKILRETI"
     gene            complement(20656..23316)
                     /gene="polA"
                     /locus_tag="PLI69_00375"
     CDS             complement(20656..23316)
                     /gene="polA"
                     /locus_tag="PLI69_00375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOG40978.1"
                     /translation="MKRDERVGKLFLIDGHSLIFRMYYAFLRRPMINSKGRDTSILFG
                     FTKYLLEMVARENPTHIAVAFDPPSETFRHKIYPDYKANRSETPELVRSSLEPLIGLL
                     EAMEIPVLITPGYEADDVISTIAVRGAAKGFDVYMVTPDKDFGQVISDHIWQYKPGKS
                     GNENEIIGKEEICAKYGISDPKQVIDILTIWGDSSDNIKGVYGIGEVGAKKLISQYGS
                     IENILEHIDELTPKQRRGFEESADHLRMSRFLVTIKTDVEVEVNEEDLRWSACFNEKV
                     RELFNEYEFNSLKSLLAEESCGQETPEIATISFEEVTPEEFEKHISGRVALRSGKRII
                     LSNGNYCCTLPPESPITKRILGNNTIDKIGFNLKNTMLTLIEHDISLEGELFDIELMH
                     YLLNPERTHKLEILVQGYLNVSLDSPLPSNARQDQPLPDLFSAHEAEESVNGLLDIEA
                     ERRLMREAVAVWQLYDIIAKEMDEESLWDLYRRIDMPLMEVLAHMEYNGVRIDTEQLR
                     SYSKELSSELATIEEEARILADEPQLNVASPKQVGVVIFEKLALDPKAKKRKNDNWPT
                     DEETLMELVHKHPFVEKVLEYRSLRKLISTYLDPLPSLVNPATGRIHTTFNQALTATG
                     RLSSVRPNLQNIPIRTERGQKIRMAFVPSSDEGYILSADYSQIELRIMAELSGDPRLI
                     ESFLHGNDIHTATAAAIFHVSPEEVTKEHRRQAKVANFGIIYGISSFGLSQRLGIPRN
                     EAKNFITKYFNTYPGVRRYMDKCIADAREKGYVTTIFGRRRYLPAITSRNHVVRGLAE
                     RNAINTPVQGSAADIIKLSMINVFRKMKSEGLKSKMVLQVHDELVFDVIPSELNRLCD
                     IVKQEMESVIKLSIPLTTECSYGKNWLEAQ"
     gene            23526..>23784
                     /locus_tag="PLI69_00380"
     CDS             23526..>23784
                     /locus_tag="PLI69_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
                     /protein_id="HOG40979.1"
                     /translation="MHTGKFVFSQLLGFIDRNDFNYLARKYDGDKYVKQFTCWNQLAV
                     LMFGQLSNRESLRDLVLATQAHASKAYHLGFGKHAVRSTLAD"
BASE COUNT         6526 a         5154 c         5467 g         6637 t
ORIGIN      
        1 atggagcaga cctcaaacga gatgtttgcc gggctgaaag ataaagagat tgaccctaat
       61 cttatggaac gccttagaga actctttaca acggctgatt ttgtgaagtt tgccaagcat
      121 accccctcca ttggggagaa tgaggaggct atccctacgg cagtaagatt tgtcaatgcc
      181 acattcttac aggaaatcga aaaagaaaag gaggagtagt taatgttttt tgaatatcca
      241 tatctgctct ggctggagtt gatactcata cctctgattg cactttacat ttggagggaa
      301 ttcagaggct cttcgccctc catgattgtc tccaatgcta ccccctggaa ggtaaaaggc
      361 ggaacattca aaaaagcagt gcgccatctc ccctttattt tgaggatgat tgcaattgct
      421 gccattattg ttgccattgc ccgcccacgc acctcggaga ctttcgaaac tgtggatacg
      481 gaaggtgtgg acatagtgct tacaatggat atctccactt cgatgcttgc ccgagatttt
      541 aaaccggacc gcataggagc tgccaaggat atagcgatag agttcgtagc ttcaagaccg
      601 agcgaccgca tcggaatagt ggtatttgca ggtgagagct ttacccagtc gccgcttact
      661 accgaccgtg tgacattgat caatcttatg aaggagatag agaccgggct gatcgaggat
      721 ggtactgcta ttggcaatgg acttgctaca gcggtggcgc gactcaaaga ctccgatgcc
      781 aagagcaaga tagtgatcct gcttaccgat ggtgtcaata accgtggtga aatagccccc
      841 ctgatggcag cggagattgc caaaacatat aatatccgag tatataccat aggggtcgga
      901 gcgatgggta cggcacctta tccggtaatg accccttggg gagttcgtct gcaggatata
      961 gaggtggaaa ttgacgaaaa gctccttcag gagatagcca cccagaccgg aggtaaatat
     1021 ttcagggcca cggataacac caaactactt gagatttatg gtgagataaa tgccatggag
     1081 aagagtaaaa ccaccgtaga cagtttcccc atctattcgg aattatttat gaagtatgcc
     1141 tcgatagcat tgctggctct tttagttgaa ctaatactta gattgtttgt aataaggagg
     1201 ataccataga atgatacacc ttgcacaagg agaatatctg ctcctgttac taatcatacc
     1261 cttcctgttt attttctacg gagtttcact ccgcataaag aaaaggagaa ttgctaaaat
     1321 cggtgacacg gcactggtat ccaaactgat gccggatgtt tccagaggta ggggatggct
     1381 taaaatcacc ctactggcga ttgcctggtt cttttttgta ttgggtctag tgaggccaca
     1441 gcttggagcc cgcctcaaag agaggcagag caagggggtt gaagtgatgg tggcactcga
     1501 cgtttctaac tcgatgcttg ccgaagatta ttctcccaac cgtctcgaga ggtcaaagct
     1561 tgcaatagaa cggctggtag accgtctcca cggtgatcgt atcggattga ttctatttgc
     1621 gggacaatcc ttcgtccagc tcccgataac taccgactat gtgtcggcta agatatttct
     1681 caatagcatt aacacaggtt cggtaccgat acagggcaca gcgctggcag atgctattgc
     1741 aacctccgcc cgtagtttta gcacccagag tgagaatagc cgtgcaataa ttatcatttc
     1801 tgacggagaa gatcatgagg gagaggtaat ggatgtggct cgctcggtgg ccgagagcgg
     1861 tatcaggatt tattgtatag gtgtcggatc accggaaggc aaacctatac ctataaaagg
     1921 agagctgcta aaggacaatt atggagagat agtggttacc cgtctggatg aaaatactct
     1981 cagacagata gccggtatag gcaatggcct ctatgtacgt gcgggtaaca gcgaatttgg
     2041 acttaaccct attattgacc atattaagga gatggatgag caggagtttc agtctgtggt
     2101 atttgaagat tttgatgagc agtatatgta ccttttcggt atagcgctct ttttcctaat
     2161 actcgaactg ctgattcccg gcaagaaagg ccgcagacaa ttgttcaaaa tggaggataa
     2221 atgatgaaaa ggataatttt gatactattt gctttctctt tggttgtgcc aacggccttg
     2281 gcgcagagtg atcgttctca ggtacgcagg ggcaacagag atttccgtaa ggaaaagttc
     2341 caggaggctg aaattagcta caaaaaggca ctggttgctg actcgctctc aaatgctgcc
     2401 ggatacaatc tcggcaatac ttttttccgt atggaaaatt ttaccgaagc ggataaatac
     2461 tactcttcag tggccgattc ccttggcaag gggaagcgcg gttccgacat ttaccacaat
     2521 atgggtaatt cgctgctaag tcagaaaaaa tttgcagagg cgatagaggc tttcaaaaat
     2581 tcgttgcgtc gcaaccccgg agatatggag accaagtcca atctggctta tgcacagaag
     2641 atgttgcagg agcagcagca gaaccaggat cagaatcagg atcagaatca ggatcagaac
     2701 caggatcaga atcaggatca gaatcaggat caaaacaaag atcaggacca aaaccaggat
     2761 caaaataaag accgggataa gaaagataac aaggatcagg ataaggacaa gaatcaggac
     2821 caggatcaga atcaagatca agatcagaac aaggacaata atgataacaa gcaggaccaa
     2881 agccaggggc agactcctcc tcccaaaata acaccgcagg cagctcagca gatgcttcag
     2941 gcaattcagg agaaagaaaa ggagacacag gagaaggtta agaaggagaa ggcccttttg
     3001 ttaaaatcaa aacagaaaga aaaaaactgg taatcacacc ggatgaaata ttatgaaacg
     3061 aacactgaca ttcctcttta tattgctggt ttcggccaag gcaacttttg gtcaggggct
     3121 taccgttgag gctccgggcg tagtggcgat ggatgagacc ttcagactgg tcttcacatc
     3181 ggatggcaag atgtcggact tcaattggcc ggggagcgag gattttaatg tagtctgggg
     3241 accacaaaaa ggcacttcca gcagtgtaag cattattaac ggcaaaagaa cttccaccca
     3301 taacgagacc gtgacttatc tgctccaggc caaaggggag ggacgtttta caatccccgt
     3361 tgcaacagct accatagata agcagcaata tagcacccgt agtgttatca tagaggtggt
     3421 gggaagtcag caagagagac aaagccaaag tcagagtcag cagaccaaca cccagcagag
     3481 tcagagtgat tcaagagtgc aaagtgcctc atccgatgac attttcctca gattgacact
     3541 aaacaagcgc aatgtagtta agggtgagcc cataatagcc acacttaaat tatataccaa
     3601 ggtggacata gcaagctttg aagatgtaaa gttccctacc ttcaatggtt tttggagtaa
     3661 ggaggtcgat acgccgcaga atatagagtt caacagggaa aatgttgacg gtactatcta
     3721 caatgcggca ttgctccgta ggtatatgct cataccccag cagaccggta agattaagat
     3781 tgagtctgct gagatggtct gtgtggttag gatacgtgca ggcaccaccg gccctagaag
     3841 cattttcgat gatttttttg acaactttca gacggttaga aaacgtctct cctctcccga
     3901 aaccacaatt acggtgaatg ctttaccgac tgctccggcc tctttcggcg gtggagtcgg
     3961 ggattttaaa atcagtacat cgttaagcaa agatagtctt actgcgcatg aggccgcttc
     4021 gttaacggta actatcagtg gaaccggcaa tatttccatg ttggaagctc caaaagtaga
     4081 gtttccctcc gatttcgagc tttatgacgt aaaaacttct gaaaaggtct cctatgacgg
     4141 cacttcaggc agtaaaactt tcgaatatcc atttatacca cgtagtcacg gtgattttga
     4201 aattgctcct attgagtact ccttctatga tctttcaggg aaacgatata aaactctaaa
     4261 taccggtaca ctctccgtta aggtgggacg tggaagcggt attgacaacg ggggagtggt
     4321 aatgcccggc gttaaccggc agagcgtaaa gaacctggct gaagatatcc gatatatctc
     4381 gacaggtgct cctttattga gagataaagg gcgtttcttt gcgggatcgc tccattttta
     4441 tggattgatt gctctcatcg tgctgctgtt ttttgccgta ggtgcaatcc tcaagaagag
     4501 agctgcgcgc cgtatggatg tgataggaac acgtaaccgc agagcaaaga aaatggcccg
     4561 ggcacgcctg caacaagcgg gatcctatct aaagcagggt cttagcggag catattacga
     4621 ggaactgcac aaggccatgc tcggttatgt ctccgacaaa ttgattatac ctgtagccga
     4681 tctttccaaa gagaggattt cctcatcact tgcagaaaag aatcttcctg aacaactgat
     4741 aactcagttc atatctattc tcgacaggtg tgaatttgca agatatgctc ctgaagtcaa
     4801 gactgaggag atggagagtc tttacgatga gtctgtaagc gttctttcag aaatagaatc
     4861 aaaattgaaa agtgtaccaa aacgtccacg gggaaacgtg gcatcaatag tagcattgct
     4921 cctattagtg actccttttg ccgtttctgc tcaggaaagt gtagatgacc tttgggctat
     4981 ggcagcagat tattatgccg aaggcgatta tgtcaatgca ctcaactttt atctgatgat
     5041 agaaggtgag cagaaggtct ctcccgatct ttattacaat atagccaaca gctacttcaa
     5101 gagtggagat aattcccacg caattctcta ttacgagaaa acactcaaac tagatccatc
     5161 acacgctgat gcggcacaca acctggccat tgcacagaga ttcactctgg acaaaataga
     5221 tatattgcct gagctgatac ttgtaacctg ggtgcgaaag gtaaaatatc tcttctcagc
     5281 agatgtgtgg gcctggattg cagttgcact tacttttgct gtggcaatgc tgatgctcgg
     5341 attccgtttc agctcttcgc tccgccgtcg taaaacctcg ttggtgctcg cttgtgtcgt
     5401 ttttctcttt gccattatct catttgcatt ctccattagt gagaaaaatg atgcaatcag
     5461 agatgaatcg gcaatagtta tgtcacctgt ggcttctgtg aaaagctcgc ccggtgaggc
     5521 aggaaactct attttcgtac ttcacgaagg aactaaagta aatctgcttg acgagcttgg
     5581 agaatggaca agaattgaac tctcggacgg acgtcagggc tggattgctg ccggatccat
     5641 aattaaaatc taatcatctg cataatgaag cgagttactt ttatactatt gttggttctc
     5701 tttgttgcga ttatgcctct cagagcacag tattattttg ccatagagga tggctctcag
     5761 gcgagtttta gggtttacga taataaagga gatgtcctgg gttattatga cgagaccctc
     5821 aaaatcgtaa gcgctgagtc acttgatgat attcatgcgg tgttggaaca ggtttatttt
     5881 gacaaggatg gcaaacatct ttttaaagac aaatcttcta atttatctct tcatatcaaa
     5941 gaaggagagg tgaccgcaaa actggagaca attaaacaag gaatgacaac aaaggacctt
     6001 atggtacgag gttgtgttat atatgttcct tcagatataa agtcaggtga catactcccc
     6061 gacagaacca tgtcaatcaa agtaggcata atcggcggca ctatctatct gactgagcgt
     6121 aaagtactcg gtatggagag tgtaaccgta cctgcgggca cattcaatgc tgccaaagtc
     6181 cgggaaaaac agaaaacaaa agttgcaatt atctcagaag aggttacaat aatctcatgg
     6241 atggttaagg acctgggttg tgtaagacag gagtcttacg atagcaaggg tcgtctggta
     6301 cagattattg agatggttaa aaagtgacgg acaacattgt aaccactcca tccattgttg
     6361 aaatcaagag cctatcctga ccgatgggtt cgatggagtt gatcaatgca acggagatct
     6421 tgtgtttcca aacgaggttt ccgtttttgc cgtcaaagca gaagatattt cccttgtctg
     6481 taggaacgat gagcatctcc ctgccatcct ttaaaaggat tgtggcagcc ggagtaggag
     6541 ctatgtcata gcccagatcg ctttgaatgt tccactccag ccgggcatcg tccgacaaag
     6601 ttgaccagca ttgtatggag ttgaacatat tcttaacgaa aatttttgac ccatcctttg
     6661 aaatagctat tgattcacga cctccgtcag cactccatac ctcttttccg gtaccggcat
     6721 ctattgcatg acactttctg gccggtgtaa ctatgaatat tttgccgtta cttttcacag
     6781 gccatacagc tgcaggggag agcatcctac tgcctttatt gcgccatacc cattgttctt
     6841 tgcccgtagc cggatcaaga gaataaaggg aattggccca gtctccgaaa actatctgct
     6901 ctttatcagc aaagggcttg cactcaacaa aacctttcac agaatcatac tgccatataa
     6961 gtttgccttt ccgtaaactt atggctttga aagaattgtc cgaagcaccg atatagacca
     7021 cattgtccat gataaccggt gaaccgagta ctgacttttt gcacttatat ttccataaaa
     7081 gtgtgccttt gcgaagattc agacagtaga tataaccatc tgtggaaccg atgaccactc
     7141 tcgaacccga tattgccgga gtggaaaaga ctttgcctcc ggtggcaaat ctccacctca
     7201 gactacctgt ggcactatcc agagctttta taactccctg tgtgttggca tagagtatat
     7261 atttgccggc ctttacggcg ccggatccga tatcactttc atcctgaatt ttccaaattg
     7321 ccttcacctg gggatatttt cggttgacat cgaatgtcaa ccatgggaag tttgcaggaa
     7381 gtgcatattc attgtttctg cctgaggctt tatccggagt gaattgaggc tcagaaaaga
     7441 tctccctcga gtaccaggct ttgccgctct ttcccccttt gccattgttg tcagccgctt
     7501 gacgctcgta gatggagata cggttgtttt cgatgttcac gatattgtaa ccgacattgc
     7561 cgttacggcc ggcagcaggg gctctcccga gtactcccgg cactccatcg taatcaagta
     7621 tggtgttgcg atgccagtgg cctcccatca caagctggat atttgtctgc ttgagggtat
     7681 tgagtacctg gaaataattg agcatcgagg tgtcctgagg gtagtggttg acaaatatta
     7741 cgggttgctt aagaggagtc acttttgcaa tggaatccaa ccaaaccaga ctctcgtggg
     7801 gcatcaatgc cggcgccatc ctcatattcg gaccgctgtt gctacccaga aacttgatgc
     7861 ctccggcttc gaattcaaaa ttttcatagc cgaatacctt tgcaaaggtg ttgcagccac
     7921 tttccgacca ctttgagtcg tggttgcctg ctacaatgta gtagggtatc tccagattgt
     7981 caataatttt tttagcaaga aaaatttctt cgtcgcttcc gaactctgtt atatctccgg
     8041 caaaaatgac aaactgcagt tccttcttca agttattgat atcgttgatg cattgcaaaa
     8101 ggtcatcgtt ggtacctgaa ccctcagcga tatgggtatc cgcgataaat gcaaacttga
     8161 ctctctcagt ctgtgctgag agcatcagag cggagaggca gagcgctgta atgaatagga
     8221 ttcttttcat attgtcactc ctgattgttg ttccattggt atctcttttc cagaggtttg
     8281 actccggtca tgtgaagtag cttgccgaca aatgagtcta cgaggtcatc gatagttacc
     8341 ggattgtgat agaaagaggg cgaagccgga cagattactg ctcctgcctc tgtaagcaac
     8401 ttcatgttgt ggatgtggat caggttcagg ggagtttccc gcggtactat tatcagctct
     8461 ttccgctctt taagttgaac atctgcaatc ctcgagatag cgtcggaggc aagtccgccg
     8521 gctattctgg cgagagctcc catggagcag gggaggagta gcagcgtatc tgcggctgcc
     8581 gacccggagc agaaaggggc attaaagtta tcattaggta tgagagtagc accacattcc
     8641 gatatcattt cctcaggaga atacctcagc tcccgcagcc agatgtttcg ggctgtcccg
     8701 gtaagtatca cactgagtga aatctcctgc acattggcac aacctcgtat atagcgctcc
     8761 gcatagataa ccccgcttgc accagtaatt ccaagtacta tctgtttttc gttacccatt
     8821 tataagttcg tttttgagtc tctccatacc ggttgtagcc ttctctacaa actgtgtaag
     8881 tcggggatgg tttagtcctg aaggtttggg gtcaataaga tagagaggga tgcctgaggg
     8941 agcgtagtgt atcaatcctg cggcgggata aactgcaaga gatgtaccta caattaccag
     9001 tatatctgcc ttggaaacaa ttctagcagc tttttctatc atgggaacag cctcaccgaa
     9061 ccaaacgatg tgcggtcgca gctgcactcc acggctatct ttgtcgttga tgctcaaatc
     9121 ggcgtaaccg atatctatgg catcgccatc gtctccatct acgggacgga ttttggtcaa
     9181 ttctccgtgc agatggatta tattggtact gccggccctc tcgtgcagat tatccacatt
     9241 ttgtgttaca acggtcacgt caaagtcttt ttccagctct gcgataacct catgtgcacg
     9301 gttgggtttt accgtttcga gttgtctgcg acgttcgtta tagaactcta ttaccagttt
     9361 aggattgctg taccatccct ctatggaggc gacgtcttct actctgtaat tctcccacaa
     9421 tccgtcgctg tcgcgaaatg tctttatacc gctttctgcg ctaatacctg cgccggtcag
     9481 aaatacaatt tttttcataa ctaatctcta ttgaaaatat gtgtttttga gccagtaggc
     9541 gaatagggtg tccaatatta tagcgttatc ctccttgtca aatgtaagta cctccttctt
     9601 tttgagtgca tctttcaggc gaaatacatt tgcggaggag ttgaggtgat atccttcgag
     9661 gatctcttga ctgctaaatc gctgcactcc gtctaccacc gcttttaaga agttgatctg
     9721 gttaggtgtc aggtccatca ttatctgcat aaaccgaggt gtgtggaggg atatgagact
     9781 atcagtggcc tttttgatga tgttattatt aacatatcct ataggcatgg acatgcagat
     9841 ggaacataag tgcttaatat accatatatt tccatcggcc agatggtata tggtgcctgc
     9901 ctgttcatcc tgaatcactc tgcccaatgt ttgaaatgat cgcacaatat agtccacgca
     9961 ctcttttttc ttgagctttt caagagaaat gacgtcacac atgaaataga aatatttctt
    10021 ttcttcaaat atgtacttca tctggttgac aaatgagccg ctgaaaatat agcgaacatt
    10081 ggtttgtttt gaccatattt tgtccaaatc ctccagatcg atttggtcat tgctgcactg
    10141 tagaaggttc tgaaactcct tgaaatagat tataatcaac tttcctgcat ctgtagctat
    10201 tatttcagga agattgagga ttttgcgttc atccagagag tctacgtgga tttcaaatgg
    10261 taatatggag ttatagtgga tttcattgta acattcgagc atcttctcct tgaaaagtga
    10321 gataaaatcc tccatagtac gtatgttgaa gaggtttatt tcacagagaa tgaaactcag
    10381 gccacgtgat tcgagtgtgt caagtgagta tcttatgaat gactccttgc cacttttggg
    10441 tgcgccatag atggcaacgc tccgtttcct atcgtatata ctcttaaaaa agagttcagc
    10501 ctcagctctt cgtcctatta tgtcgtgatt ttctgcaatg cgatcgtaat taaaaggcag
    10561 gttcatttgt agcacaaagg ttattatcac aattttgtta caaaagtaac caataggcta
    10621 ctatttggca aaaaaaaatg tagtaattgt caaaattttt gtagtaataa tcaaaattgt
    10681 tatatttgca gtccaaaaat gttgatagac aaccgttaag ctgttgaata agagatactt
    10741 aagttgtatc cgcttgcggt tataaatttt aaaatttatt ttatgtacgc aattgtagat
    10801 attgcaggac agcaattcaa agttgaagcg ggcaaaaaga tcttcgttca tcgccttgcg
    10861 gctgaagtag gtgcttctct tgactttgat aaagtattat tgactgacaa tgatggcgta
    10921 gtcaaagttg gtgcccctta tgtggaaggt gcggttgtta aagccaccgt tctcgaccat
    10981 cttaaaggtg ataaggttat cattttcaaa aagaaacgcc gcaaaggtta tcagaagaaa
    11041 aatggccatc gccaatattt gacacaaatt caaattgaaa caatcgctta attaaaaacg
    11101 tattagatta tggctcacaa gaaaggtgtc ggtagttcta aaaacggccg cgaatcacac
    11161 agcaaacgct tgggcgttaa attgttcgga ggacagagtg ccagagctgg caatatcttg
    11221 gtccgccaaa gaggaacagt tcacaatccc ggtgaaaatg ttggaatggg taaagaccat
    11281 actcttttcg ctttgattga cggtactgtt gttttcaaga aaaaggctaa taataagtct
    11341 tacgtatcag tgctccccca ggcatgacgg gaccactatt gaaaaataga gccgcttcgc
    11401 acagaagtgg ctctttttgt ttaactttgc acaccaaata attaatttat gttgacgtta
    11461 aaacttttga gagagcagcc cgaatttgta atcgcgcgtc ttgcagttaa gcatttcgat
    11521 gccaaagaga tagtgtacga tatcatagac cttgataaaa aacgccgttc ggcgcagcag
    11581 gaactggact cctgccttgc ggagcagaaa attaaagcat ccgagatcgg gaaacttatg
    11641 aaagaaggac gccgtgatga ggctgaaggg gtcaaatctc aggtagcatc tctcaaggaa
    11701 cgctccagaa ctcttgaaga ggaaatggct gctgcacagc aggagcagga tcgtaagttg
    11761 gtgctgcttc caaaccttcc tgcagcacag gtgccggagg ggcgtacagc agaggataat
    11821 gtcattgtgc gtcaaggggg cgttatcccg gatttgggag agaatgcaaa acctcattgg
    11881 gaactggtta aggagctgga tattatcgac tttgatctgg gagtcaagct tacaggtgcc
    11941 ggatttcctc tttacaaagg agcaggtgca gttatgcaga gagcattgat ggcatttttt
    12001 cttgagcgca attcatctgc cggttacata gagtaccaac ctccatatat cgtcaatgaa
    12061 gattccggtt tcggcacggg acagcttcct gataaggagg gtcaaatgta tcatatcgcc
    12121 gttgatggtt tctatttgat tcctactgcg gaggtaccat taactaatat ctatcgggat
    12181 gtcatcctgc aggattccga tttccctgtc aagatgacgg gatatacacc ctgctggcgt
    12241 cgcgaagccg gttcttatgg taaagatgtg agaggtctca atcgtctgca ccagtttgat
    12301 aaagtggaaa ttgtccgcat agagcgtcct gaaaactcct atgcagcgct ggatgagatg
    12361 gtacgtcatg ttgaatcttt ggtgcaggcg cttgctcttc cttataggat cgtacggctt
    12421 tgcgggggag atatgggttt tacttcagcc attacttacg atttcgaagt gtggtcagca
    12481 gcacagcaga gatggctcga ggtgagttcc gtgtcaaatt ttgagtcata tcaggccaat
    12541 cgtttaaagc tcagatataa ggatgaagag ggcaaatcgc agctcgccca tactctcaat
    12601 ggcagtgctc tcgccttacc gagaattctc gcctctataa ttgagaataa ccagactcct
    12661 gacgggagga tcgttgtccc tgaagtattg cgcaaatata cagggtttga gatcataggg
    12721 taatgatacg gagtgacaat ccacaaagaa taattatggg agtcgatccc ggcacctctc
    12781 ttttgggtta cggggtcatt cttgttgaca agaagaaggt ccattacgtg gatatggggg
    12841 tgatagatct gcgcaaggag aaagaccatt ttctaaagct cagaagaatt ttcaaggaaa
    12901 taggggaact tatggataaa tatgctcccg atgatctggc tattgaagct ccattttacg
    12961 gtaagaatcc gcaagtcatg ctaaaactgg gtcgtgctca aggctctgca attgctgctg
    13021 ctcttcatcg tggtattccc gtttcggaat atgctccgag aaaagtcaaa atggcaataa
    13081 cgggtcgcgg agatgcttcc aaggagcagg tgagagtttt aatggaaaaa accctcggca
    13141 ttaagatgga gagtgcattt ctggatgctt ccgatgcact tgcaatagcc atgtgccatt
    13201 tttaccagtt aataagccct ctcgtggatg tttctgcttc atccaactgg aaatcctttg
    13261 ttgagtctca tccgacaagg gtgaaaaagt gactgccgga tttaactttt gtcccatttt
    13321 caagtctaaa ataacaagct gacatatttg ataaatattt gtaaaaaaag atattatata
    13381 tatgagacaa aaagttttaa aagcggttgc attaattctg cttatatttc caacattagt
    13441 aagtgcacag cgcggaggtt ttaccgtaaa ggttactctg accgaggctg caagcggagc
    13501 cggagttgaa tttgcgacag tatcccttac tgaggcgggt tctgataagg cctataaata
    13561 tgcactcaca aatgagaatg gttttgttga gatcagaggt gtaagggccg gaaaatacct
    13621 tataagagga gaacttatgg ggtatgtccc ttatcgggat acaatcaatg tggataagaa
    13681 tctcgatctt ggtaagaaga aaatggatgt tgaggtgcat tttctggaag gagcgacggt
    13741 taccgacctg ggtaatccta ttgtaattaa aaaagatact atcgaacaca acataggact
    13801 gatgaaagtt accgacaatg agactcttga agatattctt aagcgtcttc ccggcattga
    13861 agtctcggca gacggtacta tcactgcaaa tggtaaggaa atcaagaaaa tcaccattga
    13921 cggcagaacc ttcttcctcg atgatcctca gcttgccagt aaaaatatcc ccgctaagat
    13981 aatagagaag gtaaaagttg tggacaaaca gtcggagcag gctgaattta ccggtattga
    14041 tgacggagag gaagagacca tcattgatct gagtgttatg aaaggaatga tgaacgggtg
    14101 gtttggtaat attatggcgg gagggggtag cgatcttcgt ggtaaagatg aggatggagt
    14161 ggccatagac aatgatttcc gttttcaggg agcttcaatg attgccaaat tttctgagag
    14221 cgatcacatc gcttttctcg gaaacgccaa taatacaaat aaccgtggtt ttaatgatct
    14281 cgcaggtaat ttaatggcgg gtatgcgtgg aggtggaatg cgtggaggtg gcatgcgtgg
    14341 cggtgggggt ggtatgcccg gcatgtgggg aggtcgtagt ggtatttcca cttcatatat
    14401 ggccggtctg aatgcaggta agacatttga caataaatcg gaactgacag gcaactatat
    14461 gtttaacggc aacgaaagag ttgttgagga gagtactgca aagaccacgt tcaaacagga
    14521 cggatcatca ctcttcgcta tcgatgacgg ctatagtatc accaactcct acggccatag
    14581 agccggagct cgtgccgact ggaagatttc agaacagact tccatccttt ttcagcctca
    14641 gttcaacttt ggcacaggta attttgaaga gcgttcggat tttgctaccg acaatatttc
    14701 agcctccgga cagaaatcca agatcaacga tggttacagt ttatcgacag gtgaaaattc
    14761 caaccagact gcaagaggct ttctcctctt cagacagcgt ctcggaagac cgggaagaac
    14821 catctcgcta aatatagatt atgatctctc caacaaccaa acggatggca ttaaccgctc
    14881 tctgactaac tcttataaga ataacatact tgcagattcc acactggttg accaattcta
    14941 tcaccagtca gccgagtcat acggagcagg tgcccgtcta tcatataccg aaccacttgg
    15001 taaaaatttc tttgccgaag ctacctactc ctacgattac agctatagaa actcctacaa
    15061 gacaacttac gatgattatg agaccggtgc catcatcaat catgaatata ccaatgatat
    15121 aaccaatgag tccatgttgc agagggcagg tatcaatata atgaagcagg aggagaaata
    15181 taacatcacc ataggtgcaa cagcacaacc ttcaaggact atcaatgtta cagaggtagg
    15241 tggcaattcg accactattg accgtaaagt ggttaactgg gctcctaatg cgagggtaga
    15301 cttcaatttc agcgattacg agatgttgag attcagatat aggggacgta ccactcagcc
    15361 ctcgattact cagttgcaga ccgtacctga caactcaaat cctcagagag taactcttgg
    15421 aaatccctat ctcgacccct catttacgca taggctaaat atgatgtata ataagaataa
    15481 tgtgcagaca ttctcctcgc tgatgctcaa tgcagacttc aactacaatc agcgaaatat
    15541 tgtcaatgca agctggtatg acaaaaaagg ggtacagtac actattccca tgaacaatga
    15601 caaaggtaca ttctccacta tggcaagatt gacctataac tcacctattg ccaaaagcaa
    15661 attttcagtt atgtctttct tcaatgtgag ttactctaca ggtgtaagtt ttgtgggcaa
    15721 agacgagttg ctcgacatcg acaatccatc aacttatctc aatgtgaaca attttgaaga
    15781 gaatgaatat cagaacctct ctttctcaga aaatctcaga ttcgtctatc gtgacgatat
    15841 ttttgaggtc tccgtaggtg gaaatgtgag atattcgcag gcctggtata ctattacggc
    15901 agaaaacgta agacctacct ggaccaatac cgttaatgca aatgtgatag ccaatattcc
    15961 caatattttt aatttttcga ctgacgcagc ttacacgttt tattacggtt atgaagaggg
    16021 ttataacgaa cccagccttg tctggaatgc cgaactcagc agacaaatcc tcaaaaacct
    16081 gacaataagc atcaagtgct acgatattct caaccagtcc aggaatacct tccgtacaac
    16141 aacggataac tacgttcagg atactcgcaa taatactctc ggtagatatc tgatggtgaa
    16201 tttaacttat cgtttcggca ctttcggcaa tcaatccctt ggtggtcgta tgggaggtca
    16261 tggtagaggt ggtggccccg gaggcatgta tcactaatca ctcttcctct tcgagctcgc
    16321 gaacgccggc atattttcgc cacttttcaa tcagggcggt catatccgat gggatggggg
    16381 attcaaacct catctcctct cccgtacgcg gatgtataaa tccgagactt ttggcgtgaa
    16441 gtgcctgacg tggaagtaga gtgaaacaat tgtctataaa ctgtttgtac ttcgtataga
    16501 tcgttccctt taaaatcctg tcaccgccat agcgctcatc attgaagagc ggatggccga
    16561 tgtggttcat atggacacgt atttgatgtg tacgtcctgt ctcgagaatg cactccacca
    16621 tcgttacata tccgaaacgt tctatcactt gataatgggt gatggaaggc tttccctgct
    16681 catcatcgga aaatactttg aatcttagtc tgtcgttggg gtcacgcccg atatttccct
    16741 ctatcgtacc gctatctgcc tgaatgttgc cccataccag tgctacgtat ttacgctcca
    16801 cagtccggtc gaagaactgt tttgtaagat ggaattgcgc atcttcactc ttggctacga
    16861 caagcagacc ggatgtctct ttatccagac ggtgaaccag tacgcccatc ctttcatctt
    16921 cctgatcaat gcctggagag atacccagat gataggctaa cgcatttacc agagtcccgc
    16981 ttgcgtgacc gcgaccggga tgcaccacta gtccgggagg tttgttgatt atgagcagat
    17041 cctcatcctc atagataatg tttaatggaa taggttcagg cagcacctca aacccacgcc
    17101 tctggtatgg catcatgatg gtgaccacat cgagcggttt cactttgtag ttagccttga
    17161 cgatacggtc atttaccaac acatagcccg cattgattgc aagttgtatg cggtgcctgg
    17221 aggtttgctc catacggtta gttataaact tgtctacacg cagcggcgtc aggccctttt
    17281 ccgcctcaaa gcggtagtgc tcaaacagct cctgttcctc atcctggagt aaatcgggat
    17341 cactctgcag gtcagtcatt ataatttctt ttgattgcca atttggattg gtctttagtg
    17401 aggtagatgc tcactttagt gccgcatacg aagggtagag attccgaaaa agcaggggac
    17461 tgactataaa ctaccgcctc aagggagtcg gtatagttga tgacagtctc atcaaacctt
    17521 acaagaccca catttagaga gttgtctatc aggtaatcct ttgcaaccgt atatgtatat
    17581 ccggtcagat gggggatata ggtttgggaa tcctcaggat tgagtcccag cagaagatct
    17641 atctcacttt cgctttccag aagcgtatta ggggcaatgg gtttgccctc gtatcgctgt
    17701 tccagcacat tattggtggc aatgtcttca acataaatca gcgatccgag actcaaccct
    17761 ttggaggtaa gttcagtctt ggcctgtctt agtgaaaaac caacaaggga gggcatttca
    17821 accatctgag gattaattgc attaatcgta atcagtatac ggcggccttt cttgacttta
    17881 ctgcccgcaa caggattctg tgaaaaaata tagcccctgt ccaaacgctt tacaaatacc
    17941 gaatcggtta cttcgatgcg tacatgacgt ttttctgcaa gattatgggc atcctcaagg
    18001 gacatattgg taaaatcggg aacgctaagc tccctattat gtcgtgtgat gatgtttaaa
    18061 gagccgaaaa ttattatgat aagggctgca aaaatagcga cagccagtag aatattgcgt
    18121 actatccaat tggttttcat attgcgaagt taagaaataa ttctgtaaaa actgtccctc
    18181 tcttgaatct gaaatccgga agctgaagca agctctccaa gctcttccac tgcaagtacc
    18241 ggacgttgtt cttctgaccc cgccatactg tagattttgg tactatcctc tatggtaccg
    18301 tccatatcgt ctgcgccata ctccatagca agcaggctgg ttctcttgcc gagcatcggc
    18361 caataggctt tgatatgagg aatattgtcc agcgcaagac gcgaaatggc aattgttcgc
    18421 aaaacttcct cctcagagac ctctccgatt cccgacagtg agttgttggc acaggagtat
    18481 ttgagaggta taaatgcact aaatccaccg gtatcatcct gaagctctcg taatgcgagt
    18541 aaatgagcaa tcctgtgttc cctcttttcg atatgaccga aaagcatggt gcagttggaa
    18601 gatattccca atttgtgtgc agtacggtga acttccagat aacgctctgc agagagctta
    18661 tccgggcata tctgatttct tatgtggcta tcaaaaattt cagccccacc cccggggagg
    18721 gcatcgagtc cggcttcttt tagttggatg agtacctgct ctagattgag tcccgaagag
    18781 atggacatat cctctatctc tactgccgaa taggctttaa tcgctacctc atttggcagc
    18841 agctccctta ccagggagat gatggatcgg tagtaatcaa aatccctctc cggatgtaca
    18901 cttcccacta tgtgcacttc ggtgatgccc ttactatatt tttgcaggca ataatctctg
    18961 atttggtcaa gatccatgct ccatgctccc ggttgatctt cactatcacg tctgtaggag
    19021 cagaaactac attggtgtat acagacgttg ctcggctcaa tatgaaagtt acggttgaag
    19081 aagatggtct tgccatatct cgcctcacgc tcagcaaatg cgagtgaacg aagtcggaac
    19141 atatcgcccg aatagagatc catcagttgg tcaaatccgt atctatttga attgcgcttc
    19201 atacaaccta ttattgataa tgaaaataga ccgacctgag tcagtctatc tctttttaaa
    19261 ccctatttca aatattacca gagttcttcg gatgaaacgg aaagtttctt gcgtccacga
    19321 cgcctacgtg ctgcaagtac tcggcgtccg ttcggagtgg acatgcgctc acgaaatccg
    19381 tgtttgttgc gtctttttcg ctgtgaaggt tgaaatgtac gtttcattat ttaatatttt
    19441 aatatttatc gaaattggga gtgcaaaggt aatgtttttg tttcaaaata caaatttatc
    19501 gggagattat aataatcttt ttttcggtga aatactcttc ttcaaaaaat gaggttatat
    19561 cgattatggt aattcgctcg ttctttacac gccaacccct ttgaaactct cttatttcct
    19621 cctgcaggtc gccacccttg agagatataa tggctttacg gtaggaatcc ttaacccacg
    19681 gataaagttc cctaagaggc gctactgcac gtgacaccac atagtcaaaa ctttctccta
    19741 tattttcagc cctgctattg aggcatatta cattttccaa gccgagtgac tgtgatacac
    19801 tttcggccac ccttattttc tttccgatgg aatcgcaaag tgtaaatttt gtctccggaa
    19861 agagtatcgc aagcggaatg cccggaaatc ctccaccggt gccaaggtcg aggagagact
    19921 catcgggagc aaatgacatg gccttaccta tagccagact gtgcaacacg tgatgcgtca
    19981 taaatgaacc tatgtctttc ctcgaaatga cattgatctt tgaattccat tctacgtaga
    20041 gatgttcgag tgctgcaaat ctatcccttt gaagtggcgt gagatcagga aagtatctta
    20101 atatgttttc cattctccta aattgtttcc ctcaaaatct tttttgcacg gttgatcctg
    20161 gttttcacag cacccagagt aatctgaagt tcttttgcta tctcttcata agcgtaatcg
    20221 gatagaaatc tcaattttgc aacttcacgg tatttttccg gcaggtttag gatacgtgtt
    20281 gtaaacagat ccatatcctg ctttattatc accatttctt cggggctata tgtcacggag
    20341 atatctctca aatcagcttc acctccactc agagagacgg atttaagggc gttgcgcgct
    20401 ttacggatat gatctatagc ctcattctgt gcaattatgt agagccaggt ggaaaatgcg
    20461 tattgaggat tgtatctgcc tatattcatg aaagctttct caaaactcct ctgacaaata
    20521 tcttcggcat cttcagttat ggatacatat ttgagcagaa atgcaagcaa tgcactgcga
    20581 tatttatcat acaatgcgga aaatgccttc tggtcaccat tgcgagctgc aattaccaaa
    20641 ttataatcat cgctgttact gtgcttcgag ccaatttttg ccataactgc attctgtcgt
    20701 cagtggaatg gagagcttta tcacactttc catctcttgt ttaactatat cgcagagccg
    20761 gttgagctcc gaagggataa catcgaatac caattcgtcg tgtacctgga gtaccatttt
    20821 gctttttaga ccttcactct tcattttccg gaagacattg atcatggaga gcttgatgat
    20881 atcagccgca ctaccttgaa cgggcgtatt gatggcattt ctctcggcaa gacccctaac
    20941 cacatgattg cgtgaagtaa tggccggtaa atagcgcctg cgaccgaaaa tggtagttac
    21001 ataacctttt tcacgtgcat ctgcgatgca tttgtccata tagcgccgta ctcccgggta
    21061 ggtattgaaa tattttgtaa tgaaattttt agcctcattg cggggaattc caagtctctg
    21121 ggaaagtccg aatgaggaga ttccatatat tatgccgaaa ttggcaactt tagcctgcct
    21181 gcgatgctct ttggtcacct cttcaggtga aacatgaaat attgcggcgg cagttgcggt
    21241 atggatgtcg ttaccatgaa ggaaactttc gatgagcctc ggatcaccgc taagctcggc
    21301 cattattcta agttctattt gcgagtaatc tgcagaaagg atgtagccct catcggagga
    21361 gggaacaaac gccatacgga tcttttgtcc ccgctctgtc ctgataggga tattctggag
    21421 attgggcctg acgctgctta gccgtcccgt agctgttagt gcctggttga aggtagtgtg
    21481 tatacgtcct gtagccggat tgaccagaga gggtagcggg tcgaggtaag tagatatcag
    21541 tttgcgcagg gaacggtatt ccagtacttt ctccacaaat ggatgcttat gcacaagctc
    21601 catgagagtc tcctcgtcag taggccaatt atcattcttt ctcttttttg cttttggatc
    21661 aagggccagt ttttcaaaaa tcaccacccc tacctgcttg ggagaagcga cattaagttg
    21721 gggttcatct gcaaggatac gtgcttcttc ctcaatcgtt gcaagctctg aggagagctc
    21781 cttgctataa ctacgtagtt gctcggtatc aatcctcact ccattgtact ccatatgagc
    21841 cagaacctcc atcagcggca tatcaatgcg tctatagaga tcccagagtg attcttcatc
    21901 catctcttta gcgattatat catagagttg ccataccgcg acagcttcac gcataaggcg
    21961 tctctctgcc tcaatatcaa gcaggccatt tacggactct tccgcctcat gggcagagaa
    22021 gaggtcgggt aacggctgat cctgccgtgc attcgagggg aggggggagt cgagtgatac
    22081 gtttaggtat ccctgaacga gaatttccag cttatgggtc ctctcagggt tgagcaaata
    22141 gtgcatcaat tctatatcga aaagctcccc ttccaaggaa atgtcatgct ctatgagggt
    22201 aagcattgtg ttttttagat tgaaacctat cttatctata gtgttgttgc caagaatacg
    22261 tttcgtaata gggctttccg gcggaagagt acagcaatag ttgccgttgc ttaggattat
    22321 gcgtttgcct gaccttagag ctacccttcc ggaaatatgc ttttcaaact cttcgggagt
    22381 cacctcctca aatgaaatcg tcgctatctc aggtgtttct tgaccgcaac tctcctctgc
    22441 cagtagcgat tttagagagt tgaattcata ctcgttgaaa agttccctga ctttttcatt
    22501 gaaacaggca ctccagcgca aatcctcttc attgacctct acttccacat cggtcttgat
    22561 ggtgactaga aatcggctca tccgtaggtg gtcagccgac tcttcaaatc cccggcgctg
    22621 ttttggggtg agttcatcaa tatgctccag aatattttca atcgagccgt attgagaaat
    22681 aagtttcttc gcccccacct cacctatgcc atacacgccc tttatgttgt cggaagagtc
    22741 tccccaaatg gtcagaatgt caatcacctg ttttggatca cttataccat atttggcgca
    22801 aatttcttcc ttaccgatta tttcgttttc gttacccgac ttacccggtt tatattgcca
    22861 gatatggtct gaaataactt ggccaaaatc tttgtcgggc gttaccatgt atacgtcaaa
    22921 tccttttgcc gcacctctga ctgcaatcgt ggaaatcaca tcgtctgcct catagcccgg
    22981 ggtaatcagt accggaattt ccatggcttc gagcaggccg ataagcggtt caagagagct
    23041 acgtaccagt tccggagtct cgctgcggtt ggctttatag tcgggataga ttttgtgtct
    23101 gaatgtctct gaggggggat cgaaagctac agctatatgc gtagggtttt ctcttgcgac
    23161 catttccagc agatatttgg taaagccgaa caggatagag gtatcacggc ctttgctatt
    23221 aatcataggc cttctcagaa atgcataata catcctgaaa atcagtgagt gtccatcgat
    23281 aaggaataat ttcccaactc tttcatcccg tttcatctta caaatgtaag aaataaataa
    23341 ggttttccag caaaaggctt tccggaaaaa tatttgctgt tattaaaaat gagttgtgaa
    23401 atatcactag tgtccggtaa aaaaccataa aagttctttg aaaatattga aaattaggta
    23461 attacgaggc tttttcgagt caacggattt gaaattatct ttgccagact aacgtagaat
    23521 ggcacatgca tacaggaaaa ttcgtgttct cacagctttt aggctttatt gatcgcaacg
    23581 attttaacta cctcgcaagg aaatatgacg gtgacaaata tgtcaagcaa tttacctgct
    23641 ggaatcaact cgcggtactg atgtttggtc agctttccaa ccgtgaaagt ctcagagatc
    23701 ttgtgctggc aacccaggct catgcctcta aggcatacca tcttgggttc ggtaagcacg
    23761 ccgtcaggag tactcttgct gatg
//
LOCUS       DAOSIE010000004        16980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_3572_length_16980_cov_4.025938, whole genome shotgun sequence.
ACCESSION   DAOSIE010000004 DAOSIE010000000
VERSION     DAOSIE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16980
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3572_length_16980_cov_4.025938"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..345)
                     /locus_tag="PLI69_00385"
     CDS             complement(34..345)
                     /locus_tag="PLI69_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG40980.1"
                     /translation="MRPKIDTDKLKYLPTAEEMFTDEYGAKGTASRDEFDAKSLAWYY
                     SEVLKNARKSAGMTQKQLADKIGKKREYIAMLEKGETDMQLSTFIMIADAVGLKFALT
                     Y"
     gene            complement(345..674)
                     /locus_tag="PLI69_00390"
     CDS             complement(345..674)
                     /locus_tag="PLI69_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG40981.1"
                     /translation="MRTIIRSIEFDEFYNSLPANVQNRVQYVLNIIAEVKVVHKKFVK
                     KLVNTNFYELRISVGSEYRVILFAIDHENFIEAHQILLLNGFIKKSTKDYDKEIKKAQ
                     QILNTLQ"
     gene            complement(742..1611)
                     /locus_tag="PLI69_00395"
     CDS             complement(742..1611)
                     /locus_tag="PLI69_00395"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOG40982.1"
                     /translation="MRKIFNKIALSVLITLFSVTLSAQNTDSLTFVTAKAVANAEKNG
                     IVFNNYLFEGNLFNSNQCISLLEVPSGYHFDVVAAPEGTLVWTSLLSMGAKAVAALNG
                     SFFNMRPPFGGVTYTRVDGTIVAENNKDVSTPFWSRSIRQNGAIAILGGELYVLKADQ
                     LRNWEKYIFAEDVLTSGPILMVDGALEPQAPDKFNTTRHPRTAVGKRSDGTVVFVVVD
                     GRFPEAAGVSTTELSLVMSWLGCRDALNLDGGGSSTMVFDGNIVNHPSDNKKFDHQGE
                     RVVANAIVVRDAD"
     gene            complement(1812..2492)
                     /locus_tag="PLI69_00400"
     CDS             complement(1812..2492)
                     /locus_tag="PLI69_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOG40983.1"
                     /translation="MNKVAENPSKNRTVPYPIPLVIVSAVFVTLYLVSNIMAVKIVGI
                     GKWIYFDAGTITFPFAYMLGDVLTEIWGYKTAKRVILLTLACNILLVICTWIGVFLPV
                     PDYNDAIASAYNRVFTYVPRLVLGSIAGFLCGELSNAWLMVKIKKWTEGRWLWVRTIG
                     SSIVGHLFDTVPFVLIAFGGVVPWIDILKLIAFQFVAKLAIEAIMGTPLAYLILGWMR
                     RFGITSHS"
     gene            complement(2580..3533)
                     /locus_tag="PLI69_00405"
     CDS             complement(2580..3533)
                     /locus_tag="PLI69_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOG40984.1"
                     /translation="MKAMNKKCLYCYKALNAEGDFHPSCSAHFFGTHEPPTLDYTMSE
                     MAELAKEVVEQSITVPGVQPKLSLGLIREVLQDGSRGRLTIMGALGGNYILKPQNEIF
                     PQMPENEHLTMRMAELCGISVVPSTLIRLKSGELSYLTKRIDRTETGEKIHMLDMFQI
                     LEAFDKYKSSVEKVGKAVAKHSSNTLLDLLRLFEVVLFSYITGNNDMHLKNFSLILEK
                     EDWILSPAYDLLNVNLHLPEDTEEMALTINGKKRKLTKSDFIKLGLGFQLTEKQIFNT
                     FKRFVEAEGAMKNEIKSSFLSSGNQAKYIELLESRLNLLKE"
     gene            complement(3517..3852)
                     /locus_tag="PLI69_00410"
     CDS             complement(3517..3852)
                     /locus_tag="PLI69_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HOG40985.1"
                     /translation="MRQGKVFYKDNFAGTITETDDGDYFFEYDKEYIDLFPNQPITFS
                     MPVSNKKYKDKRLFPFFDGLIPEGWLLEIASRSWKLNQNDRMGLLLACCKNCIGAVSV
                     IPINNESNE"
     gene            complement(3849..4061)
                     /locus_tag="PLI69_00415"
     CDS             complement(3849..4061)
                     /locus_tag="PLI69_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG40986.1"
                     /translation="MTLSLAAFVKTKRKEANLTQQEFADRAGVALTVVRKIEQGKENL
                     SLEKVNQVLMMFGHKLAPVNHKKIEP"
     gene            complement(4285..4971)
                     /locus_tag="PLI69_00420"
     CDS             complement(4285..4971)
                     /locus_tag="PLI69_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOG40987.1"
                     /translation="MQRIINEIKAYIDPEKARILPRFFKTGKGEYGEGDRFMGVTVPN
                     IRKVAKSNKDVSLELVEKLLQSEWHEVRMCAVLLLVEKFKKQENKVLEIYLRNTARIN
                     NWDLIDLSAPQIAGRYLLDKSDRSLLYRLAKSESLWERRIAIVSTLTFIRNGQFEDTI
                     AISERLLGDPHDLIHKATGWMLREMGKRDLTLLRSFLSKHSGTMPRITLRYAIEKIDE
                     EERKFWMNRR"
     gene            complement(5224..5577)
                     /locus_tag="PLI69_00425"
                     /pseudo
     CDS             complement(5224..5577)
                     /locus_tag="PLI69_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            6100..6486
                     /locus_tag="PLI69_00430"
     CDS             6100..6486
                     /locus_tag="PLI69_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40988.1"
                     /translation="MNIKRIFGLLTIACAITLMCGFSSCSEKPDEDLIFSYTANGNIS
                     ASSGDAVESLFCIAEYSEAIAKVLGQTFSMTERDKDVINACDDVFLKHRINHPEWKGY
                     VEIEKSRVGILGEKLSSVVLKTYKYE"
     gene            6501..7451
                     /locus_tag="PLI69_00435"
     CDS             6501..7451
                     /locus_tag="PLI69_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HOG40989.1"
                     /translation="MKKIFIALINATIIFSSGCKESQKPTYIPQAPDYTRESMWYNVL
                     NDTTGEGADVFYIPSTWEFDWSTEDGKISHYADVYNQAHTDNMTIEMKGVADYMAEGN
                     NFYSPFYRHITLDSWATLNEDTIARRYHSVSFVDVQSAFDHFVANYNNERPFILAGFS
                     QGAKSVVELMKTMPAETRSRMIAAYVLGYKVTPDDTALAPWIVPAQDSLDTGVTICYN
                     SVSDVKYTKPILCSDNIMCINPVNWRTDSTPAVLHDTITVTLNPQYKVLVLEGFDGSY
                     LPNILDMVNVGDYHGIEPWIYSDCLRKNFKQRIKAYKEAN"
     gene            complement(7448..8212)
                     /locus_tag="PLI69_00440"
     CDS             complement(7448..8212)
                     /locus_tag="PLI69_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOG40990.1"
                     /translation="MKSIIIALLIPLLGTVLGSAFVFVMRKEMPALLQKALLGFASGV
                     MVAASVWSLLIPSIEMGNGKMAIVPAVVGLLLGFAFLLLIDYITPHIHASGASEGPKS
                     GLSKTTKLALAVTIHNFPEGMAVGVAIAGATLSDFNIAGALALSVGIAIQNIPEGAII
                     SMPLRAAGNSKWRSFLIGAASGIVEPIGGALVLLLASGILGILPYMLSFAAGAMLYVV
                     VEELIPDASKGEHSNIGTIGFAIGFALMMVLDVVLG"
     gene            8662..9561
                     /locus_tag="PLI69_00445"
     CDS             8662..9561
                     /locus_tag="PLI69_00445"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG40991.1"
                     /translation="MEKSEYNTLYQSEVIHDVADTDSTIVVATYNIGYLSGLTNNLPI
                     YASPEFIANNEVRVIEALRELDAHIIGFQEIEFGSSRVYGTNQGTMICDSLYAYGAFA
                     INWDKRYVPFPYWPPKVHFGKVISGQAVMSKFPITEQDVHQLEGVKGSSYFYREIYAN
                     RLAQVVSIDHPVKPFLVINLHAEAFEKETRLKQLDYVYELFWELEKDIPVILLGDFNS
                     NLHEEDAKIYLFLNDERLVSCAVFRDAFTSSPNSFPTDNPDRRFDYIFFNPEDFELID
                     ARIATEFGDISDHFPCVATLRIK"
     gene            9610..10536
                     /locus_tag="PLI69_00450"
     CDS             9610..10536
                     /locus_tag="PLI69_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG40992.1"
                     /translation="MKKKEDYKLKYFPWRWGDRRDGWRVRNVDPLAEMIPYFFRKRMD
                     SQIYFEATFPIDEIEAFVKKHKEEIPGLSLMHVIIAATVRVFSQRPKLNRFISRNKLY
                     ARKYINVSLIVKKSMTDEGEEDWIKIYFHPTDTLYDVVKKVQQALDEAHTEGKQDTGN
                     IAINILSILPSFLKRFMVSTLVWMDKLGVAPRAFEEGAPFFTSVFLSNIGSIGAESVY
                     HHLYEVGTCSMFMAMGTKSTQHITRRTGEVQTFKTIKMKFTFDERIADGFYFASSIRT
                     AFRISQDPEQLLTPPEQVIVDDGVGRKRKDQP"
     gene            10606..10992
                     /locus_tag="PLI69_00455"
     CDS             10606..10992
                     /locus_tag="PLI69_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG40993.1"
                     /translation="MKLLCPYCGEEISDKAIKCKYCGEWLTNDQNVRNSTVQPQVVIR
                     PVERKTNGAGTAGFVIALISAIVFWLPFVSQILWVFGLIFSFIGLFKRPRGLAIAGFI
                     ISFVNLIVILVIILVFGLVLGGIFAK"
     gene            11180..12430
                     /locus_tag="PLI69_00460"
     CDS             11180..12430
                     /locus_tag="PLI69_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG40994.1"
                     /translation="MKTKFYNAKIYRNKTATEFIVEKGKITQIGTNLPKCDKEIDLKG
                     KLVLPPYVDPHLHLDYVYTLGEMEGIGAASGTLFEAIENWPKYKATMTKESVKNLAKK
                     GILDEVSQGVQIIRAQTDVTDPKFTGFKAIMELKEELKHIVDIQVVAFPQNGMYTYKG
                     GRDLVEEALKMGADVIGGIPHYEPAREFGEKSIHDIVKLALKYNVLIDVHCDETDDPM
                     ARFVEILNALVYLEDYGTKATASHTCSFGSADDSYAYRMFDMFKRSKLNFVSCPTENA
                     YLQGRQDTYPKRRGLTRVKEFMEMGINVAFAQDSINDPWYPLGSGNMMNVLDNGIHLS
                     QFASPEEVEVAFDLITYNGARCLNIHDKYGIEVGKDANFIVLDGDSPWDCIRRRVGVL
                     ASVRKGEFLFKKKPTEFDVPIVKF"
     gene            complement(12501..13673)
                     /locus_tag="PLI69_00465"
     CDS             complement(12501..13673)
                     /locus_tag="PLI69_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG40995.1"
                     /translation="MNKEKKSTGIFPWVLMILMSSVTFVGILSELMPSGVLPQMMADL
                     GIDEIQGGRLVGFYAIASAIFGIPLISATMRYNRKVLLLLLLVGFAVCNIASALVHNY
                     YLVLSLRFLGGIAAGVMWPMIAAYGMRLVDPHQQGKAVAVIMAGNTLGISLGMPLMTS
                     IGERFGWRIEFIVLGLIVVLIAVLSIFMLPSTPGEKLTRSTSPFAMLKSKPILLIILL
                     TFLGVCAHYGVYTYITQLVAEIGIAGGIQIALLVFGIGSLISIVIATKHIDKYLRQLT
                     VLMFLFIGIAMLIFYFFKGTMVISHIGFFLWGLSFGPLVTLFQAAVGNQVATAKDVAM
                     SVQSCMFNLSIMITTWIAGVLLVHYGAFSLIWYAVILAVPGIIISHFAKHTLRPMG"
     gene            13867..14232
                     /locus_tag="PLI69_00470"
     CDS             13867..14232
                     /locus_tag="PLI69_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOG40996.1"
                     /translation="MKQRLTSKEEEVMNIIWENGEMFIRDIVALMPEPKPNYNTVATQ
                     VKFLEEKGFVERRAMANSFQYRAIVSEREYRGTRVGALVSKYYDNSYASVVSQFVEDE
                     KMDLNELKRLIARIEKKRK"
     gene            14229..15494
                     /locus_tag="PLI69_00475"
     CDS             14229..15494
                     /locus_tag="PLI69_00475"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOG40997.1"
                     /translation="MIPWLLKSGVLLAIFYLFYLLFMRKSTFFRFNRIAIFVGSAICF
                     ILPLINVGSFLPTEAMPRIELPTLVVGGDLPLEAPSAINWKNILLIIYIIGAAVVLAI
                     TAFSVLKTFSILRKGERVTLKECKLTIIDSKIPSFSFLNHIAISREDYERNPVILSHE
                     LQHIRCRHTIGILLFAVVTVLQWFNPLVWILRTELKMLHEYEADEAIIKQGIDATQYQ
                     LLLVKKAVGAHRFQMANGFNHAKLKNRITMMQSTKSNKFKRLLYLACLPLIVFSLSFC
                     NRQKEKATDPAVEIEISDETKAPATVEIKVKESTQDEDTFPLAEVTEKPTFNGKDANE
                     FAKWVNTQLVYPEDAMAKGEQGRVTLKFTITKEGYVTNVKVLRGLSESLNAAAISAVE
                     ASPKWTSPGKINGKPVNVTYIFPVLFQLR"
     gene            15497..16687
                     /locus_tag="PLI69_00480"
     CDS             15497..16687
                     /locus_tag="PLI69_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG40998.1"
                     /translation="MHLLRILFVSVFLALLPTFVTAQETDSLALRQNTINAAKYLKSI
                     YKIDEAIDTLSTLVGLTFDEEVLSELADCHYQNGDLENAAGTYLLLTSVNPNNILYKV
                     RQVAIFYKLKAYGQAVEVGKEVLRIDPISAIAGLIGDSYNQMGQYDSALVYYNQTLAL
                     KPFNESVVSKVAKIYLDRKEYPEVIAISDAFLEFSPDNTNIAPVKGLALYLHGQYFPA
                     IDIYEKQLKLGNDTYSIHYYLGQCYWRTLLLYRAERELKAAWQLDSSDVNLAYSIAGV
                     LADANRPFDDVKPWLDKALAMLEPDSSVLSRIHQQYGLGYYKKQDSWDQAIEHYKKAY
                     NYNPQFISALSTIAFCFEQKKDYKSALEWYEKYLAVAKPGSSGYKFAQKSIEILKGEL
                     FMEE"
BASE COUNT         4977 a         3525 c         3608 g         4870 t
ORIGIN      
        1 cgagtttttt taggatggag tagaaataag tattcaatag gtcaaagcga attttaagcc
       61 gacagcatcg gcaatcatta taaatgtaga gagttgcata tcggtttcgc ctttttcgag
      121 catagcaata tattcgcgct tttttcctat tttatcggca agttgcttct gggtcattcc
      181 cgcagattta cgagcatttt tcaatacttc tgaatagtac caagcaagag acttcgcatc
      241 gaactcatca cgcgaagcag ttccttttgc tccgtactca tctgtaaaca tctcctcggc
      301 ggtaggaaga tatttgagtt tgtctgtgtc aatctttggt ctcattactg taatgtgttt
      361 aatatttgtt gtgctttttt aatctcttta tcataatctt ttgtggattt ctttataaag
      421 ccattcaaaa gaagtatctg gtgagcttca ataaagttct catggtctat ggcaaataga
      481 ataactctat attcacttcc aaccgatata cgtaactcgt agaaattcgt gtttacaagt
      541 ttctttacaa acttcttgtg tacaacctta acctctgcga taatgttcaa gacatattgt
      601 actctgtttt gaacatttgc gggtagagag ttataaaact catcaaattc tatacttctt
      661 atgattgttc tcatgcgcaa atgtaataaa tttattacat tttgcaaaaa aaatgcaaaa
      721 aatgcctaaa ggaaggtata actagtccgc atcccgaacc actattgcgt ttgccacgac
      781 tcgttcgccc tggtggtcga attttttatt gtcggatggg tgatttacga tattgccgtc
      841 gaaaaccatt gttgaggagc cgccgccgtc cagattgagt gcatcacggc atcccagcca
      901 gctcattacc agactcagct cggtagtaga aactccggct gcttcgggga atcggccatc
      961 tacgaccaca aatacaactg tcccgtcact gcgtttacca acagcagtac ggggatgtct
     1021 ggttgtgttg aatttatcag gtgcttgtgg ttcaagtgcg ccgtcaacca tcaggatagg
     1081 acctgaggtg agcacatctt cagcgaagat atacttttcc caattcctga gttgatctgc
     1141 cttgagcaca tatagctctc cgccgagtat cgcaattgct ccgttctgac gtatacttcg
     1201 tgaccagaag ggagttgata catctttgtt gttctcagct acgatagtcc cgtctacgcg
     1261 ggtataggtg actcctccga atggaggtcg catattgaaa aatgaaccgt ttagggcggc
     1321 gactgctttt gctcccatcg aaagtaggga tgtccataca agcgtccctt ccggtgctgc
     1381 aactacgtcg aaatggtatc ctgacggtac ttccagtaat gaaatgcact gattggagtt
     1441 gaataaattc ccttcgaaga gataattatt gaatacaatt ccatttttct cagcatttgc
     1501 gacagctttt gcagtgacaa atgtgagcga atcggtattt tgtgccgaaa gtgtgaccga
     1561 aaagagggta atcagtacac tcagggctat tttgttgaat attttacgca tggctttatg
     1621 ttattgagag atgtgtctat ttagtgttat tatagtaggt acaatgtaat cgtcaaacaa
     1681 gaacaaatgt ccctgatgtc acaaatataa gatttattcg ccttataatt cataatacgc
     1741 aactcgcaac ctgcaccccg taacactgag aactccggac taattactta ctcggaactc
     1801 tttactcaga cttaggaatg actagttatt ccaaatcggc gcatccatcc caggatgaga
     1861 taggccagag gtgttcccat tatagcttcg atggcgagtt tggcaacaaa ttgaaaggca
     1921 atcaatttca gaatgtcaat ccagggtact acgcctccaa aagctatcag cacaaaaggg
     1981 actgtatcaa agagatggcc gacaatagag ctgccgatgg tacgcaccca gagccaacgg
     2041 ccctctgtcc actttttgat tttcaccatt agccaggcat ttgatagttc tccacagaga
     2101 aaacctgcga tggaacccaa aacgaggcgg gggacataag tgaagactct attatatgcc
     2161 gaagcaatgg catcattgta atccggcacg ggaaggaata cacctatcca ggtgcagatc
     2221 accaaaagga tattgcatgc caaagtaagc aatatgaccc gcttggcggt cttatagccc
     2281 catatttcgg taagtacgtc accgagcatg taggcgaatg gaaatgttat ggtgccggca
     2341 tcgaaataga tccatttgcc aattcctacg atttttactg ccatgatatt ggacaccaga
     2401 tagagtgtca cgaagaccgc agagacaatc accaatggaa tgggataggg gactgtgcgg
     2461 ttttttgaag ggttttccgc taccttattc atattcagtt aaatattttg tgcaaatgta
     2521 gtaatttcgg tggaattaga atataagata tgattctact tagcaccagg ccaaatgact
     2581 tattccttca ataaattcaa tctgctttca agcaattcaa tatattttgc ctgattcccg
     2641 gaagaaagga atgaagactt tatctcattt ttcatcgctc cctcggcctc gacaaatcgc
     2701 ttgaaggtat tgaaaatctg cttttctgtt aattggaagc ccaatcccag tttaataaag
     2761 tcagactttg tcagcttcct cttcttacca ttgatggtga gtgccatttc ttcagtatct
     2821 tctggaagat gcagattgac attcaataaa tcatacgcag gagagagtat ccaatcttcc
     2881 ttttccaaaa taagcgaaaa gtttttcaga tgcatatcat tattgcctgt gatataacta
     2941 aaaagcacta cctcaaaaag tctcagcaaa tccagtaatg tgttggaaga gtgttttgca
     3001 accgctttcc caaccttttc tacagagctt ttatatttat caaaggcttc cagtatctga
     3061 aacatgtcca gcatatggat tttttctccc gtctcggtac gatctatacg ctttgtaagg
     3121 taagagagtt cacccgattt taatctaatc agagtagatg gaacaacgga aatgccgcaa
     3181 agttcggcca ttctcatagt cagatgctca ttttccggca tttgaggaaa aatttcattt
     3241 tgaggtttga gaatataatt tcctcccaaa gcacccatta ttgtcaatcg tcctctacta
     3301 ccatcttgca aaacctctcg aatcaagcct agagatagct ttggctgcac cccgggaacg
     3361 gttatcgatt gctcaacaac ttctttagct aattctgcca tttccgacat tgtatagtct
     3421 aaggttgggg gctcatgggt tccaaaaaaa tgagcactgc aagaggggtg gaagtcacct
     3481 tccgcattca acgctttata gcaatataaa cacttcttat tcattgcttt cattgtttat
     3541 aggaattaca ctcaccgcac caatgcagtt tttacaacag gccaaaagca agcccatacg
     3601 gtcattctgg ttaagcttcc agctcctgga tgcaatttcc agcagccaac cctccggaat
     3661 caacccatca aaaaagggga ataagcgttt atccttatat tttttattag aaacaggcat
     3721 agaaaaggtt atcggttgat tggggaagag atcgatatat tccttatcgt attcgaagaa
     3781 ataatcgcca tcatccgttt cagtgattgt tccggcaaaa ttatctttat aaaacacttt
     3841 accctgtctc atggctcaat ttttttatgg ttaacggggg cgagtttatg cccgaacatc
     3901 atcaggacct gattaacttt ctccaagctg aggttttctt tcccttgctc gatttttctg
     3961 actacggtaa gggctacgcc tgctctatca gcaaattcct gttgcgtaag attggcctct
     4021 ttccgttttg ttttaacaaa tgcagctaaa ctcaatgtca taattatatg cttttgcagt
     4081 tatttttggc aaatataaga aattatatgt aaaagcatat aaaaacagta ataaaagtga
     4141 aattatatgt aaatgcatat aaaatgagtt aagtattagg aactatatgc gaatgcatat
     4201 agtttaggct ttagaaacga ttttgtatgt gttaggatat aatcaatgag cgaataagga
     4261 ggaggagcct ttttggtagc taatttatct tctattcatc caaaacttcc gttcttcctc
     4321 atctatcttt tcgatagcat atctcaaagt gatgcggggc atcgtgccgg agtgcttgct
     4381 tagaaacgag cgcagaagtg tgagatccct tttgcccatc tcacggagca tccatcctgt
     4441 cgctttgtgg atcagatcgt gaggatcacc cagcagcctc tctgatatgg caatagtatc
     4501 ttcaaattgg ccgtttcgga taaaagtcag agtgcttaca atggcaatgc gccgttccca
     4561 taaagattcg ctcttagcaa gtcgatagag tagggaacgg tcgctcttat ccaataaata
     4621 cctacccgca atctgcggag cacttaggtc tatcaaatcc caattattaa tcctggcggt
     4681 attgcgaagg taaatctcga gaaccttatt ttcctgcttc ttaaacttct caaccaatag
     4741 cagcacagca cacattctca cctcgtgcca ctcgctctga agcagctttt ccaccaattc
     4801 cagcgaaaca tccttgttac tctttgccac cttgcgaata ttcggtaccg tcacacccat
     4861 aaacctatcg ccctcgccat attcaccctt tcctgtcttg aaaaacctcg gcaatatacg
     4921 agccttttcc ggatctatat aagcttttat ttcattaatt atccgttgca tttcccattc
     4981 ttgattgaag agttgttgga tggttttagg gtttttgagg agaaattgga attctttcag
     5041 tttcgcagca tttggagatt cgggaatcca gagttttaag tagccgcctt ctgcatattt
     5101 attggccatg tggtcgagaa atctctcgaa atgttcctct ttatcgaggg tagggtagtg
     5161 tttaagcccg tattggatag tgcgtctgat tatggttgag ccgtcaatac ctctgtctgc
     5221 ttctgcaatg attttaccat atattgaacg gggctcgtgt tcattagagg ctcggtgatc
     5281 ctccgctgca tcagccatga tctcaatttg cttaggagaa aaccatttct gcaaagcctt
     5341 atcctctcgt attatgcgcg cagactcaat atggtgcgtc tcccgatcaa cttccagccc
     5401 caaatcgtga taggccgcaa tcacatatac catctcggca ttcacatcat aatgcttcgc
     5461 cagttttgcg ctctccttta tcacttttaa ggcatgatcc ctctgatgcg ccgcatcaaa
     5521 tgaatcgtat cgaggaagga tcttttcttc gatatattgc tttagagaag aattcatgga
     5581 gtaaaggtaa ggaatttttg gctttggcat ctatttccat ttccctggga tagtataagt
     5641 ttttatcccg ttcgtcacga agtccgacat gattgcattg gcaaatttga tttcactttt
     5701 ctccataaat cttaaaaaat aaaaagccgc taccggagcg gcttaaacta tacaagaaag
     5761 tatagcgggg tttgttttac aaagatagat aaatttgcgc actttaaata tattctgata
     5821 ttaatctgtt tctaggttgc tcataattca taatatgaac tgatgagaat catatccctt
     5881 caaactgttg caaaatgtaa cggttttgta atcatcagaa catttattaa ataggcttcc
     5941 ttaatcggat cactttcctt gcgtagcaat gcagaaaatt aggtagccgg atggtcaaac
     6001 attagattta taattattac atacaagaaa gcatactaat tgagaaaata gtcgtaaatt
     6061 ggatgattga aatattataa atttaaaaca ttcctaacca tgaatataaa aagaattttt
     6121 gggcttttga ccattgcgtg tgccattaca ttaatgtgtg gtttcagctc atgttccgaa
     6181 aaacccgatg aagatttaat cttttcatac actgccaacg gtaatatatc ggcatcatcc
     6241 ggtgatgctg ttgaatcgtt attctgtatc gcagaatatt cagaagcgat tgctaaggta
     6301 ctggggcaaa ccttttctat gacggaaagg gataaagatg ttattaatgc atgtgatgat
     6361 gtttttctaa aacacagaat taatcatccg gagtggaaag gatatgtgga aattgaaaaa
     6421 tccagggttg gcattttagg tgagaagctt tccagtgtgg tcttaaagac ctataaatat
     6481 gagtagttat ggaattgaaa atgaagaaaa tatttattgc gcttattaac gccacaatca
     6541 ttttttccag tggctgtaag gaatcacaaa aaccaactta tattccgcaa gcgcccgatt
     6601 atacccggga aagtatgtgg tacaatgttc tcaacgacac taccggagaa ggtgcggatg
     6661 ttttttatat tccttccaca tgggagtttg attggagtac cgaagatggc aaaatctctc
     6721 attatgccga tgtctataat caggcgcata ccgacaatat gaccatcgag atgaaagggg
     6781 ttgcagatta tatggcagaa gggaataatt tctattcacc tttttaccgt catataaccc
     6841 tggattcgtg ggccactctc aatgaagata cgatagcgag gagatatcat agtgtctctt
     6901 ttgttgatgt gcaaagtgcg tttgatcatt tcgtagccaa ttacaacaat gagcgtccgt
     6961 ttatattggc gggatttagt caaggagcta aatctgttgt ggagttgatg aaaaccatgc
     7021 ctgctgagac aagaagcagg atgattgcag cttatgtact cggttataaa gtgactcctg
     7081 atgatactgc tcttgcccca tggattgtgc cggcacagga ttccctcgat acgggagtta
     7141 ctatctgcta taattccgta tcggatgtaa aatacacgaa gccgattctt tgcagtgaca
     7201 atattatgtg cataaatccg gttaattgga gaaccgattc cacccctgcc gtgttacatg
     7261 ataccataac cgttacgctc aatccgcaat ataaagtatt ggttctggag ggttttgatg
     7321 gcagttatct gccgaatatc ctcgatatgg tgaatgtcgg cgattatcat ggcattgagc
     7381 cctggattta cagtgattgc ctgcgtaaga atttcaaaca aaggataaaa gcctacaaag
     7441 aggcgaatta acccaaaacc acatccaaca ccatcatcaa tgcgaagccg atggcgaaac
     7501 ctattgtacc tatattagag tgttcgccct tggatgcatc cgggatcaat tcttccacca
     7561 ccacatagag cattgcgccg gcagcgaaag agagcatata gggcagaatg cccagaatgc
     7621 cggaagccaa caacaggaca agtgcaccgc caataggttc cactattcca cttgctgcgc
     7681 caatcaggaa ggagcgccac ttgctattgc cggcagcacg cagaggcatg gagatgatag
     7741 ccccctccgg aatattttgg atagcgattc cgacagagag cgccaaggct cctgcaatat
     7801 tgaaatccga gagcgtcgca ccggcgatgg cgacaccaac cgccatacct tccggaaaat
     7861 tgtgaatagt gacagccagc gccaacttgg tcgtcttcga aagcccgctt ttgggtccct
     7921 cagaagcacc actcgcatgt atatgggggg ttatgtaatc tatcagcaga aggaatgcga
     7981 agcccagcag aaggccgact accgcaggaa cgatggccat cttgccattt cccatctcta
     8041 tggaagggat cagcagcgac caaacggagg ctgcgaccat gactcccgat gcaaagccga
     8101 gcaacgcctt ttgcagcaga gccggcatct cctttctcat cacgaaaaca aatgctgagc
     8161 cgagtacagt gcctaataaa ggtatgagaa gtgcaataat tatagatttc ataacggttt
     8221 aaatggggaa ggagtcaata ttgatcgtct gtaattgtgc atttggtgcg gtaaagataa
     8281 ggattaatct caaagtaggg agaataaaaa ttgacttctg tatgcacctg gattagaaga
     8341 tgcaccaaat cggcggagtc agaaacaatc tacaatgata tatgtgagag agaaatggtg
     8401 gttaataaga gtttttttgt aattttgaac taataaatag gaaatgattg cagatggccc
     8461 ctgccggaca atatccctat catcctatgc ttaaaaatga gtgaaaattt taacctttta
     8521 tctgatatga ctatgaaaac atgtgattca aacgagagac aatgttgtag ttgtggtaaa
     8581 atttgggcta tcatattgat cgtaatagcc gttattgcaa ttcttgtatt tggatttttc
     8641 ttttgggcaa gatccggcaa tgtggagaaa tcggagtaca acactctgta tcaatctgag
     8701 gtgatacacg atgtggcaga tacggatagc acgattgttg tcgccaccta caatatcggt
     8761 tacctgtcag ggttgaccaa caatctcccc atatacgctt ctcccgaatt tattgccaac
     8821 aatgaggtgc gagtgataga agctctgagg gagttggatg cccatatcat agggttccag
     8881 gagattgaat tcggtagcag ccgcgtgtac gggaccaacc aaggcacaat gatatgcgac
     8941 tcgctctatg cttatggagc atttgccatc aactgggata agcgttacgt acccttccct
     9001 tactggcctc cgaaggttca tttcggaaaa gtcatctccg gacaggccgt tatgagcaaa
     9061 ttcccaatta cggagcagga tgtacaccaa ttggagggtg ttaagggcag ttcctatttt
     9121 tacagagaaa tttacgccaa tcgtttggca caggtggtaa gcatagacca ccctgtgaag
     9181 ccatttctag ttataaacct tcacgcagag gcttttgaaa aggagactcg tctcaagcag
     9241 ctcgattatg tgtatgaact cttttgggaa ctggagaagg atattcctgt aattctcctg
     9301 ggagatttca actccaatct acatgaggaa gatgctaaaa tatatctctt tctgaacgat
     9361 gagagattgg tttcttgtgc agtattcaga gatgccttta cttcgtcacc caattcattc
     9421 ccaaccgata atcccgacag acgtttcgat tatattttct tcaacccgga ggattttgaa
     9481 ctgattgacg cacgaatcgc caccgaattc ggcgacatat cggatcactt cccttgtgta
     9541 gcgacattga ggataaaata aggagaatag acggaataat tatttaattg aaaccattat
     9601 ttcttattta tgaaaaaaaa ggaagattat aagttaaagt atttcccctg gaggtggggt
     9661 gaccgtagag atggttggag ggtacgcaat gtcgatccgt tagctgaaat gataccctat
     9721 ttctttcgga aaaggatgga ctcgcaaatc tactttgagg ctacattccc gattgatgag
     9781 atagaagctt ttgtaaaaaa acacaaggaa gagatcccgg gcttgtccct aatgcatgta
     9841 attattgcag ctacagtaag agtgttttcc caacgcccaa agttaaatcg cttcatctca
     9901 cggaataaac tttatgccag gaagtacatt aacgtatcac ttattgtaaa aaaatccatg
     9961 accgatgagg gcgaagaaga ttggattaaa atttattttc atccaacgga taccttgtat
    10021 gatgtcgtta agaaagttca acaagcgtta gatgaggcac atactgaagg gaaacaagac
    10081 acggggaata ttgctattaa tattcttagt attttacctt cctttttgaa gcgcttcatg
    10141 gtttcaacac ttgtttggat ggacaaactg ggagttgctc cgcgtgcatt tgaagaaggt
    10201 gccccattct ttactagtgt atttttatcc aatatcggct ccataggcgc agaatcagta
    10261 tatcaccacc tttacgaagt aggaacatgc agcatgttta tggcgatggg tacaaaatcg
    10321 acccagcaca taaccaggcg gacaggcgaa gtacaaacat ttaagaccat aaaaatgaaa
    10381 ttcacatttg acgagcgaat agctgatgga ttttattttg cttcttctat aagaaccgct
    10441 ttccgcatat ctcaagatcc ggagcagtta ctcacgccac ccgaacaggt tattgtagat
    10501 gacggagtag ggaggaagag gaaagatcaa ccctgatgtg agatttttca aatatttcta
    10561 tttatttgcc aaatttggtt tttgaaatca aatcgtaaat ctattgtgaa actactctgt
    10621 ccatattgtg gtgaggaaat aagcgataaa gcaataaaat gcaaatattg cggggaatgg
    10681 ctgactaatg atcagaatgt aaggaattct acggtgcagc cgcaggttgt catcagaccg
    10741 gttgagagaa agactaatgg ggcggggact gcggggtttg tgattgcatt gatttctgct
    10801 attgtcttct ggttgccatt tgtaagtcag attctttggg ttttcggtct gatattttca
    10861 ttcatcggac ttttcaagcg ccctcgtggg cttgctattg caggattcat aatatcattt
    10921 gtcaatttaa tcgttattct tgtgattatt ctggtttttg gcctggtgtt gggtggaatt
    10981 ttcgctaaat agctcttgac cgacctcaat aattagccaa taattataga atccgaactc
    11041 gtgaacacct ctcacgagtt ccctttctat cactcacgaa cccacccaaa atccgcacat
    11101 tttttcttca aaaattatag ttttattata aataaatgag taaatttgaa gatatatttc
    11161 attttttaat ttctcaggta tgaagacaaa attttataat gcgaaaatct atcgcaacaa
    11221 gactgcaacg gagtttattg ttgaaaaagg caaaatcact caaatcggaa caaatctacc
    11281 caaatgtgac aaggaaatcg atttgaaagg taaattggtt cttcctcctt atgtggatcc
    11341 tcaccttcac cttgactatg tttacacatt aggggagatg gaaggcatcg gagcagcatc
    11401 cggtactctt ttcgaggcta tcgagaactg gcctaagtac aaagctacca tgaccaaaga
    11461 aagtgtaaag aatctggcta agaaaggtat cctcgatgaa gtttctcaag gtgttcagat
    11521 tattagggca caaactgacg ttacggaccc caaattcaca ggtttcaagg ctattatgga
    11581 gctcaaagag gagttgaaac atattgtgga tatccaggtg gtagctttcc ctcagaatgg
    11641 tatgtacacc tataagggcg gtagagatct ggttgaagag gctttgaaga tgggtgccga
    11701 tgtaatcggt ggaattccac actacgagcc ggcaagagag ttcggtgaga agtcaattca
    11761 tgacattgtg aaacttgctc tcaaatacaa tgtattgatt gacgttcact gcgatgagac
    11821 tgacgaccct atggcaagat tcgttgaaat cctgaatgcg ttggtatatc ttgaagatta
    11881 tggcacaaaa gcgaccgcga gccatacttg ttcattcggt tcagctgatg attcttacgc
    11941 ttacaggatg tttgatatgt tcaagaggag caaactgaac tttgtgtctt gccctacgga
    12001 gaatgcttat ctgcagggaa gacaggatac ctatccgaag cgtcgcggtt tgaccagagt
    12061 taaggagttt atggagatgg ggatcaatgt tgcatttgca caggactcca tcaacgaccc
    12121 ctggtatcct ctcggaagcg gtaatatgat gaatgtcctc gacaacggta tccaccttag
    12181 ccaatttgca agccccgaag aagtagaagt tgcttttgac ttgatcacat ataacggagc
    12241 aagatgtttg aatatccatg ataaatatgg aattgaggta gggaaggatg ccaacttcat
    12301 cgtattggac ggcgacagcc cctgggattg catccgcaga cgtgtcggcg tgttggcttc
    12361 agtaagaaag ggagaattcc tattcaaaaa gaaaccgaca gaatttgatg ttcctatcgt
    12421 taaattctaa ttaagtaggt aatattaatg aaaaagccac ctttttaggt ggctttttct
    12481 attcaacata aaacccaatt ttatcccata ggacgcaggg tgtgtttcgc aaagtgcgaa
    12541 ataattatac cgggaaccgc caatatcact gcgtaccata tcagactgaa cgcaccataa
    12601 tgtaccaaca gtacacctgc aatccaggta gtaatcataa tggaaagatt aaacatacat
    12661 gactgcaccg acattgccac gtcttttgca gtagccactt ggttaccgac agccgcctgg
    12721 aagagtgtta caagaggacc aaacgacaaa ccccacagga agaatccgat atgagagatg
    12781 accatcgtac ccttgaagaa atagaatatc aacattgcta ttcctataaa gaggaacatc
    12841 agaactgtaa gttgacgcag atatttgtcg atatgctttg ttgctatcac tatggatatc
    12901 aaggaaccaa tgccaaatac caataatgct atctgaatac caccggcaat gccgatctct
    12961 gccaccaact gagtgatgta agtataaact ccgtagtgtg cacaaacgcc gaggaaagta
    13021 aggagaatta tcaacaaaat gggtttgctc ttaagcatag caaaaggtga tgtgcttctt
    13081 gtaagttttt cgcccggagt ggatggcagc ataaagattg aaagaacagc aataaggaca
    13141 acaataagtc ccaatactat aaactcgata cgccagccga atcgctcgcc tatggaagtc
    13201 attaaaggca tacccagact gataccaagt gtattacctg ccataatcac agcaacggct
    13261 ttcccttgtt gatgagggtc aaccaaacgc atgccgtagg ccgcaatcat aggccacatt
    13321 acaccggcag cgatacctcc gagaaaacgc agagacaaaa ccaaataata attgtgtacc
    13381 agagctgagg ctatgttaca tacggcaaag ccaaccaata atagcagcaa caatactttg
    13441 cggttataac gcatggtcgc cgaaattagc ggaataccga aaatcgctga ggcgatggca
    13501 taaaagccca ccaaacgtcc gccttgtatt tcgtcgattc ccaagtcggc catcatttgt
    13561 ggaagtactc ctgaaggcat caactcagac aaaattccca cgaaggttac tgaagacata
    13621 aggatcataa gtacccaggg gaatatcccg gtagattttt tttctttatt catatgctaa
    13681 gtttttaata catatttata ggcttaaagt taacaattat ctcgcacata tttttattta
    13741 ttttgaaaaa ttattattgt ccggttaagt aaaaactaat ttatctcgta aaactcctaa
    13801 aataattagg agttatcatt tttttatcta tctttgcggt agataacgtg gataaccgga
    13861 attaatatga aacaaagact tacatcaaaa gaggaagagg tgatgaatat catctgggaa
    13921 aacggggaga tgttcataag ggatatagtt gctctgatgc cggagccaaa accgaactac
    13981 aacaccgttg ctacgcaagt caagttcttg gaagagaagg gatttgtgga aagaagggct
    14041 atggctaact cgtttcagta ccgtgcaatc gtctctgagc gagagtatcg cgggacacgc
    14101 gtcggtgcgc ttgtatcgaa gtattatgac aattcctacg cgagtgtggt gtctcaattt
    14161 gtagaggatg agaaaatgga cctcaatgag ctgaagagac tgattgcacg aatagaaaag
    14221 aaaagaaaat gataccctgg ttactaaaaa gcggtgtgct gctcgcgatc ttctacctgt
    14281 tctacctgct tttcatgcgg aaaagcacct tttttcgttt caaccgcatt gcgattttcg
    14341 tagggagtgc aatatgcttc attcttccgt tgatcaacgt cggctctttt ttgccgactg
    14401 aggcaatgcc gagaattgag cttccaacac ttgtagtagg tggagacctc cctcttgaag
    14461 cacccagcgc cataaattgg aaaaatattc ttttaataat ttatataata ggagcagcgg
    14521 tagtgctggc tataacggca ttttccgtgc tgaagacatt ttcgattctg agaaaagggg
    14581 agagggttac tcttaaggag tgcaaactca ctattattga ttccaagatc ccttctttca
    14641 gctttcttaa tcatatagca atcagcagag aggattacga gcggaatccg gtgatactca
    14701 gccacgagtt acagcacatc agatgccgcc acaccatcgg catccttttg tttgctgtcg
    14761 ttacagtact ccagtggttc aatccgcttg tctggattct ccgcaccgaa ttgaaaatgc
    14821 tccacgaata tgaggccgac gaggccatta tcaaacaagg catcgatgct acacaatatc
    14881 aacttcttct cgtgaaaaaa gctgtgggag ctcatcgctt ccaaatggcc aacggcttta
    14941 atcacgcgaa actaaaaaat cgtattacaa tgatgcaatc tacaaaatcc aacaaattta
    15001 agagacttct ctatcttgca tgtcttccac taattgtatt tagtctctct ttttgcaacc
    15061 ggcagaagga gaaggcaaca gatcctgctg tggagattga gatttctgat gaaaccaaag
    15121 ctcctgctac ggtggagata aaggtaaaag aatcaactca ggatgaggat acatttccat
    15181 tggctgaagt aaccgagaaa cctactttca acggaaaaga tgccaatgaa tttgccaaat
    15241 gggtgaacac gcaactcgtc tatccggaag atgcaatggc aaagggggag cagggtagag
    15301 taacgcttaa attcactatc acaaaagagg gttatgttac caatgtaaaa gtccttcgcg
    15361 gtctttccga atctctaaat gcggcagcaa tcagtgctgt tgaggcatca ccgaagtgga
    15421 cttctccggg taagattaat ggaaaaccgg ttaatgtcac ctacattttt ccggtactat
    15481 tccagcttcg ataacgatgc atttgctccg aattctcttt gtttccgtgt ttcttgcgct
    15541 gttacctacc tttgtaacgg cgcaggagac ggattccctg gccttacggc aaaacacaat
    15601 caacgctgca aaatacctta aaagcattta taagattgat gaagcaattg atactttgtc
    15661 tactttggta ggactcactt tcgacgaaga agtcctttcc gaactcgccg actgccatta
    15721 tcagaatggt gatttggaaa atgcagccgg gacctatctt cttttgacta gcgtaaatcc
    15781 gaataatata ctttacaaag tacgccaggt agcgattttc tataaactta aagcttacgg
    15841 ccaagctgtc gaagtaggaa aggaggtact tcggattgat cctatttccg cgatagcggg
    15901 ccttataggg gattcttata atcaaatggg gcagtacgac tccgccctgg tatattacaa
    15961 tcaaacgctg gcactcaaac catttaacga gagtgtggtc agcaaagttg ctaaaatcta
    16021 tcttgaccga aaagagtatc ctgaggttat tgccatttct gacgcatttt tagagttttc
    16081 tccggataat acaaatattg ctccggtaaa agggcttgcg ctctatttgc acggtcaata
    16141 tttccctgcg attgatatct acgaaaaaca gttgaaactc ggtaacgaca catactccat
    16201 acattactat cttgggcagt gctattggcg cactttgcta ctttataggg ctgagcgaga
    16261 acttaaggcc gcctggcaac tggactccag cgatgtcaac ctcgcttatt ccattgccgg
    16321 tgtgcttgca gatgcaaata gaccttttga tgatgtcaaa ccctggcttg ataaggctct
    16381 ggcaatgctt gagcccgatt cttctgtttt gtcaagaatt caccaacaat atggcctcgg
    16441 ctactacaag aaacaagatt cttgggacca agccatagaa cattataaaa aagcctacaa
    16501 ctacaatcca cagttcatct cagcactctc tacaatagcc ttctgcttcg agcagaaaaa
    16561 agattacaaa tcggctcttg aatggtatga gaaatatctc gccgttgcca aacccggctc
    16621 cagcggctac aaatttgccc aaaaaagcat agaaatcctg aaaggagagc tgtttatgga
    16681 ggaatagggc agggggtgcc tgtttgccat tagccattga ctgttggctg ttggcgatta
    16741 gcggttatat tcaataaagc tcaaacgagt ttggcttgac aacagtgttc tttatatttc
    16801 cgtgcatttc ccgctgctac gcagctctgt gcagccctgt ggctgcgaaa tgcccggcaa
    16861 tgattttctc gtcacagtca agttcaagca agcttgactt gacgttcggc tcgtcattat
    16921 ttgcctatac agtctataaa tctaattgat aatcaattag ttaagtcgtt cgtggttcaa
//
LOCUS       DAOSIE010000005        15743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_3901_length_15743_cov_3.899669, whole genome shotgun sequence.
ACCESSION   DAOSIE010000005 DAOSIE010000000
VERSION     DAOSIE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15743
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3901_length_15743_cov_3.899669"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1580)
                     /locus_tag="PLI69_00485"
     CDS             complement(93..1580)
                     /locus_tag="PLI69_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG40999.1"
                     /translation="MKRNLLLLIVATFVLFGCKSTQPEGNFSNIEHPEWVKNAVIYEV
                     NIRQYTPEGTFAAFEQHLDRLQQLGVDVLWLMPIHPIGEESRKGTLGSYYSIKNYCEV
                     NPEFGTMEEFRHLLEAAHQKGFKVILDWVANHTSRDAKWTVEHNDWYYKDSLGNLAVQ
                     YDWTDIARLNYENQDLREEMIAAMKYWIELGVDGFRCDVAAELPTDFWNDAVSRLKEI
                     NPDIFMLAEAEKAELQYDAFNAHYTWDQMHNWYALAAGTIDADSIANFYVNYQNRSGM
                     PSNTIPMNFTTNHDQNSWYGTCTEMYGPAVRQFAVLSFVVPGMPLIYSGQEAGLDKRL
                     EFFEKDVIDWGADQGNMADFYRELIEMRDKHACLWAQPWGGVMAILPSDRPQEIFVFE
                     REVEGDLCLAMFNFSDQTVTFNVENHLVTRDSEFTLPPHGYHIIFSIGECFGSCDECK
                     EGCDCGDCDDCDDGRKCEGCVDCDHNDKSVEAKEKTEVKAETTEK"
     gene            complement(1589..3673)
                     /locus_tag="PLI69_00490"
     CDS             complement(1589..3673)
                     /locus_tag="PLI69_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG41000.1"
                     /translation="MKPDFFILRKLVLAILPAIYLLSGCSQKEFSFDYAEHLGNMTPC
                     IVEQDTTIVILRDYFPKMKDMDTLTIITSADAPFLVMYECYDTVDGVEKFCPLLVKNA
                     IPAKLEGVDRSKAPKMSTLPAGKSGKDFFIKIEGGMEQLVVLWEGRILERGRHFDLLS
                     DSLVRVRTPRAARKLERSHIQAFVSNASGVGNDIFVPLEFGKVIRDPAQLKRTDKHSQ
                     ILYSLLIDRFVNGNSANDWSIGDPSLVHPKVNYWGGDLAGVTAKIKDGYFSDLGINTI
                     WLSPITQNPYDAWGYAPDPGTKFSGYHGYWPIFATCIDKRFGTEDELNELLAEAHAAD
                     MNVILDYVSNHMHIESPTLKAHPDWTTPSYTPDGRKNRELWDEFRLTTWFDDHIPSLD
                     LEREEICRQLSDTALFWMQNYDFDGYRHDATKHIPEGYWRLLTRKILDSLPGKSLYQI
                     GETYGSLSLISLYVKPGMLDGQFDFNVYDTFIWATTRPEGSFEGLAGCIEGNLGVYGH
                     HNLMGYISGNHDRPRYISLASGDVRHDEDQKLAGWKRDIVVSDTMAYRKLAMLHALNL
                     TLPGVPCIYYGDEYGQPGANDPDNRRWMEFEGYSRYEQQVADEVRKLSHLRRSSMPLI
                     YGDYFSLAAENDIFVYMRSYMGEAVIIFLNKSGDSREISVLLPFGLEYEGSTSVSVTL
                     EPHSYLIIETNK"
     gene            complement(3684..5726)
                     /locus_tag="PLI69_00495"
     CDS             complement(3684..5726)
                     /locus_tag="PLI69_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG41001.1"
                     /translation="MRRKIIFFLALTLTLFATGSSCNPTVNPDMQEFKEVMAYPPQWD
                     GTKRGDITYQLLVYSFADSDGDGWGDLRGITEKLDYIQSLGASAIWLSPIHPSASYHG
                     YDVEAYGVINPRFGTMADFKQLVSEAHKRNIHIYLDYVINHSGVNHIWFKIATSSMKN
                     TYRDYYIFSNDPQKDIAAGKIPMIATEGAGGYDSGQWFSTGTMPSGIYKFTLNWSNGN
                     APTVTISSGGTPDKDNPDQTTEGAKYLYYGDGICKKFYSKGNGVYELTVDFNSSWGFL
                     IRTSNSSSWPLGTKYGAKKGTSGQLSFDVPFTLYTDNSSNDNILNLTLPGGNIYYYHS
                     HFWTDWFADLNYGPVATAEFSLAFKEIVAIAQGWINTGVDGFRLDAVKHIYHNENSDE
                     NPQFLKKFYDIVNTHFKAKNGRDIYMVGEVFSDWDKVAPYYKGLPALFDFSFWWRMNA
                     AINQGQGLGFADATITIHKAYSNQRSDFIAATKLSNHDENRTRSELGGSLAKAKLAGA
                     MLLSSQGSPYIYYGEELGYTGTKTGGDEYVRTPMYWGDRYVTSYTDKIDPAVGSTVGS
                     VVSQEADASSILTMYRQLGRLRNSCPALATGKMERHPVINPQTGGIYSQVAAWYMIEG
                     KQKLLVLHNLGQGEITLSLSESLTKPLFANGLVQTKKVKENFQLKMEGYSSVIFEL"
     gene            complement(5886..8444)
                     /locus_tag="PLI69_00500"
     CDS             complement(5886..8444)
                     /locus_tag="PLI69_00500"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HOG41002.1"
                     /translation="MKKILSFIAVAALMLGITSCEVFPEDAFSTAPVAPELYSHSDIL
                     MTSNTMDEDINFSWSAYRFLPEGLNYDFYAEYEGTAAKLASTKTLYYIASKSDFKTIL
                     YNTFSSLPENNTFTMSFFVQVTDGANTYKSETLTIDIYAFGDAVVPVFGEVIKSVTLD
                     PADPTGNIMLFTWEPARLGYNEEITYNVYAQFEDGDAVELASELKETSYSTTVDAFND
                     AIVSTGAPEDATSEVKFFVKAFSETYASGVPSAVITIPVKTYIASYPDILYLPGSHQG
                     WNPATAPMMYESTIAKGFYEGVIDLRTEDGSDVEFKFCIKPAWGDDFGGIVKVSTFAG
                     EYASATGAVGAPDNIKVPSGIYNIALNKKLNTIYMVQIGSLGVIGTAVGGWSSDINLV
                     YDAEKHTFSAPVDIVPGEFKFRFNDDWTHSIGGTGLNNVSSMIGNNLNYEGPEGNYKI
                     VLNVSKTPYTVTFINTAYPDNLYIPGNHQGWNPGTAPVLTGDGEGHYEGFAHLDGEFK
                     FTHERNWDNDFGGTFDALVLKGSNFNLPKGYYYLSVDLTELKATALLITRVAVVGSFC
                     GWGSPEDAAMTYDAASNSWKISGLVFPANTEYKFRMNAGWDLPGAYNLGGEPANLVYG
                     GANLKDAEGGTYDIELILSEKPFRAVITRTGDAPAPAYASYVTVPGSYSGHSWNPTDD
                     VALYQAGTEGLYKGAIAMYGGSNQFKFHEAGAGWISGQLVAGTTYEFNLWSGDNMEVA
                     DGTYFWTVDLPAKKAVATPLTRVAIVGAFAAGDDWGDGVAMAFDAATRTYSATVEFKA
                     GSEFKFKFNNNWDYNLGSGDKGLVFNGGNIKVAEAGTYKVTLDIANTAKYVVTK"
     gene            complement(8464..9570)
                     /locus_tag="PLI69_00505"
     CDS             complement(8464..9570)
                     /locus_tag="PLI69_00505"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HOG41003.1"
                     /translation="MKQFKYIVMLMMALFLFSACEMEIEHVMVDPAQSVAPTLLAQKD
                     IIVNKDNSKVEAVVFNWTSASFGVPTQIQYALHLTLGEYTVLAGTSFSNSFTISKQDL
                     NGLVCNDLKVKPNETANISASLTASIYGTVVAPVKSNSISFNIKTYAADLRYLFLPGN
                     YQSWNITQAPQFWETDGGTNLYKILVDLQDGSADYSYFKVTVARNWSDANWGYNYLTP
                     SWSCPEQSDSNLSVDLRDGSIYELTVNRSAMTIDKKVVDGVGMVGAYNGWSYATTGDD
                     PLKYDATENVWVSPAVTFKESDGLGFLIRLNTAHDPNNWTFKFGTNGEASSDVPGGIK
                     LVQGGGDIFVPSAGTYIMKLYGNRTPYVLVMEKQ"
     gene            complement(9602..11272)
                     /locus_tag="PLI69_00510"
     CDS             complement(9602..11272)
                     /locus_tag="PLI69_00510"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG41004.1"
                     /translation="MNKILYKSFLALLASMILATSCIGDLDTQPLNETDATSETAYKD
                     EVSYLKSLGYIYGYWALASQNDAGSSDINVSDAGQSELARMYMILNELSTDALKIIWG
                     DNYINPIQYHKWTSADNEATIAVYTRCMKGITLVNEFLVRTTPEMLASRGHEAFQKTI
                     DQYRAEARFNRALYYYLLMDFFGNPPFAMPEHIGGDLPEQIGRTELFKWIEGELKELS
                     ALADMPDKGTVPYPRATKGSVWALLARMYLNAEVYTGTARWQDAKDAAAKVIGMGYKL
                     HDNYHELFMQDNTTNGAAEEFIFAISYDKDQTQSWGGTTHLVSATLSGDANAAIGAAV
                     VGEGQLINPENWNGYHVADDYVARFELVNVKWGTNGFGYDRAASDKRAFFFNGGATKE
                     FDNTTTDSGWRCWKFSGLYSDGHAVSSAETNYKFSSIDFPVFRLPEMYLIYAEADARL
                     NGGNVTDETAKGYIRQLRQRAGVDATTPSIMNLNWLLDERARELMWEGHRRVDLIRYG
                     YFTSMQFPWTLKGGVPNGKIALPDYRTVYPIIISDLNANPKLVQNPGY"
     gene            complement(11287..14280)
                     /locus_tag="PLI69_00515"
     CDS             complement(11287..14280)
                     /locus_tag="PLI69_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG41005.1"
                     /translation="MKRLMTMLLSVMVLSVTAAFAQYTAKGVVVDSKGETIIGAAVIQ
                     VGTANGTQTGLDGDFVLTVPSGETMLEISFLGYKTVTITAAQASAARIVMQDDVEFLE
                     EVVVIGYGTVKKSDMTGSVVAIKADEINRGAITSPDQMLLGKVSGLHVTPASGQPGAS
                     ATIRIRGAASLNASNDPLIVIDGVPVTSDGGAGMGNPLASVNPNDIESYTVLKDASAT
                     AIYGSRASNGVIIITTKKGRAGARGVNVSYNSSYSVKQNASTIDMMDADTFRNFMQST
                     YANNNTIQTLLGNESTDWQKQIYRLAFNTDQAVSVYGATKHLPYRVSLGYNYDQATLK
                     VGDNQKANVDISLSPKFFDDHLSINLNVKGIYQKTNWAPTGAVGSALSFDPTKPTHFT
                     NADGSIDYSKVANGYWNWLNADGSANTMAGTNPLSSLYDFTDVNKGLRSIGNLQVDYM
                     FHGFEDLRFNLNLGYDIAKSNGEKFNHPGSISAMRSSLDLYTDYANYNSNTLLEAYFN
                     YKKELGKSNLDVMAGYSWQHNYVRYDQSTYLNNEARPLYQANPTNAKEYYLISFFGRI
                     NYSLLGRYLFTATIREDASSRFSKANRWGFFPSVAFAWNVAEESFLKGSDAVSALKLR
                     LGWGRTGQQDIGSDYYPYMARYEESSSIAMKYYLGTGYYTTLAPQKYNPNLKWETTET
                     YNLGLDFGFMNDRITGSLEGYYRYTYDLLNEISTPLGSNFGNSIISNIGNMENKGVEL
                     SLNVIPVETSDMSLSIGGNVTLQSTKITKLTSVETDGYLGVTTGSGMGGTGGYTSLHR
                     TGFAPFTFYLFQQLYDSQDNPIQNGLVDRDGDGVITDADRYVTGFKPAPDAYFGLNLK
                     FRYKKWDFGFNGHGSFGNYIINRVAMSYASSYSDNWNKGYLDNLSNIYLVDGWTAAME
                     NNQKYTDMFIENGSFFRMDDINLGYTFDKVKWVESIRLSASVQNVFVISKYRGLDPEL
                     QASDGVDSNIIPRPRLFTLRLNINF"
     gene            complement(14302..15639)
                     /locus_tag="PLI69_00520"
     CDS             complement(14302..15639)
                     /locus_tag="PLI69_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG41006.1"
                     /translation="MKRPKMSFWGIFNMCFGFLGIQFGLALQNANVSRIFQSFGADVS
                     TLSLFWLAAPLTGMIVQPIIGHYSDRTWTRLGRRRPYFLVGAILASLMLFLMPNSPAL
                     APFLSPLLIAAGVLMIMDASINVAMEPFRALVADNLPSEQRTLGFSVQTFLIGIGAVC
                     GAVLPFVLTNWFGMSNVPESAGGVAPNVKLAFYIGGIVLFASVMVTVVRTKEYPPEPG
                     AKKEKDGGFIEIFRDFAKMPKTMRQLGLVQFFSWFALFSMWVYMTPAVADHYYGTVDT
                     ASAAYGEAGDWVGVLQAIYNGVAAIIAFLLPVTADKIGRKATHAISLILGALGFASFL
                     FFKNPTMLIISMVGIGIAWGSILAMPYAMLAGSLPPKKMGVYMGIFNFFITIPQICNG
                     LIGGLMVKHIYGGQTIYALLFSGISLLIAAISVFFVDDKDDPVQLFQRKTIKQ"
BASE COUNT         4177 a         3764 c         3647 g         4155 t
ORIGIN      
        1 cagcatggcg gttccactct ctcaatttcc tgtgcatatc ccgctgcact tataagcagc
       61 aggagcaaga gtgcacttat cctgcgcata gactatttct ctgttgtttc agcttttact
      121 tctgtttttt cttttgcctc aacactctta tcattatgat cacagtctac acacccttca
      181 cactttctac cgtcatcgca gtcatcgcag tcaccacagt cacacccctc cttacattca
      241 tcacagctac caaaacattc acctatacta aaaatgatat gataaccgtg tggaggcaat
      301 gtaaattccg aatccctggt cacaagatga ttttcaacat taaatgtaac tgtctgatcg
      361 gaaaaattga acattgcgag acaaaggtct ccttcaactt ctctttcgaa aacaaatatt
      421 tcctgtggtc tgtcggaagg cagaatagcc atcacaccac cccacggctg agcccagagg
      481 caggcatgtt tgtcgcgcat ttcaataagt tctctgtaaa aatcagccat gttgccctga
      541 tctgcacccc agtctattac atctttctcg aaaaactcga gacgcttgtc cagacctgcc
      601 tcctggccgc tgtaaattaa aggcattccg ggaaccacaa atgaaagaac cgcaaactgc
      661 ctgacagcag gaccatacat ttcagtgcag gtgccatacc aggagttctg atcatggttg
      721 gtagtaaagt tcatcggaat cgtattggag ggcattccgc tcctattttg ataatttaca
      781 tagaagttgg ctatggagtc cgcatcaatt gtcccggcag ccaaggcgta ccaattgtgc
      841 atctgatccc aggtatagtg tgcattgaat gcatcgtatt gcaattctgc cttttcagcc
      901 tcagcgagca taaagatatc ggggttgatc tcttttaaac gtgagacagc gtcattccag
      961 aaatcggtgg gcaactcagc tgccacatcg catcggaaac catcaacgcc aagctctatc
     1021 caatatttca ttgcagcaat catctcctct ctcaaatctt gattttcata attgaggcgt
     1081 gctatatctg tccaatcata ttgaacggca agattgccca gggaatcctt gtaataccaa
     1141 tcattgtgtt ccacagtcca ttttgcatcc cttgaagtgt ggttggcaac ccaatccagg
     1201 attactttga agcccttctg gtgggcagcc tcgaggagat gtctgaactc ctccatcgtt
     1261 ccgaattcgg gattgacctc gcaataattt ttaatggaat aataactgcc tagagtccct
     1321 ttacggctct cctctcctat aggatggatc ggcataagcc aaagtacgtc gacacccaac
     1381 tgttgcagac ggtcgagatg ttgttcgaat gccgcaaatg tgccctccgg agtgtactgg
     1441 cgaatattca cttcgtagat aacggcattt tttacccatt cgggatgctc aatattggaa
     1501 aaattgcctt ccggctgtgt ggatttgcaa ccaaagagta caaaggttgc aacaatcaat
     1561 aataagaggt tacgtttcat agatagtatt atttattagt ttcgataatc aaataagaat
     1621 ggggttccag cgtcacgctg acagaagtgg atccctcata ttcgagtcca aaggggagta
     1681 agacggaaat ctcgcgtgag tcccccgatt tattgaggaa tatgatgacg gcctcaccca
     1741 tatatgaacg catatagaca aagatatcat tttcagctgc aagcgaaaaa tagtcaccat
     1801 atatcagcgg catcgaagag cggcgcaggt gagacagttt cctgacctca tccgctacct
     1861 gctgctcata gcgactatag ccctcaaatt ccatccagcg cctattgtcg ggatcgtttg
     1921 ccccaggctg accatactca tccccataat agatgcaggg tactccgggg agtgtcaaat
     1981 tgagagcgtg aagcatagcc aacttacgat aggccatcgt gtcgctcact acaatatctc
     2041 gtttccaccc cgcgagcttc tggtcctcat catgcctgac atcacccgat gcgagcgaga
     2101 tatatctagg acggtcgtga ttgccggaga tgtagcccat aagattgtga tggccataga
     2161 ctccgaggtt gccctcaata caacccgcaa gaccctcaaa agagccctca ggacgcgttg
     2221 tcgcccaaat aaaagtatca tatacattga agtcgaactg accgtctagc atgccgggct
     2281 ttacatagag ggagatcagc gatagcgaac cataggtctc ccctatctga tagagacttt
     2341 ttccgggcag ggagtccagt atctttctgg tcagcagacg ccaataaccc tccggaatat
     2401 gtttggtggc atcatgccta taaccgtcaa aatcataatt ctgcatccag aaaagtgcgg
     2461 tatccgagag ctggcggcag atctcctccc tctcgaggtc cagagaggga atgtggtcat
     2521 caaaccaggt agtcaggcga aactcatccc ataattcgcg gttcttgcga ccgtcaggag
     2581 tgtatgaagg ggtagtccag tcgggatggg ctttaagggt tggactctca atgtgcatgt
     2641 gattggatac atagtcgaga atcacattca tatctgctgc gtgggcctcc gccagcagct
     2701 cgttgagctc atcttcggta ccgaagcgct tgtctatgca ggtggcgaag attggccagt
     2761 aaccgtggta tccgctaaac ttggtacccg gatcgggtgc atatccccat gcatcatagg
     2821 gattctgagt aatcggcgag agccaaatag tattaatacc caagtcgctg aagtaaccgt
     2881 cctttatttt ggcagtaact cctgccaaat cccctcccca atagttcacc ttgggatgca
     2941 ccagcgaggg atcgccaatg ctccagtcgt tggcactgtt gccgttgacg aaacggtcta
     3001 tgagcagcga atagagtatc tgtgagtgct tatcggtacg ctttagttgc gcaggatctc
     3061 tgatgacctt tccaaactcg agtggcacga agatgtcgtt gcctacccct gaagcgttgg
     3121 agacgaaagc ctggatgtgg gagcgctcca acttgcgtgc agctctgggt gttcttactc
     3181 ttaccagcga gtcgctgagc aggtcaaaat ggcgtccgcg ttcaagtatg cgaccctccc
     3241 ataggactac caactgctcc attcccccct ctatttttat aaagaaatct ttcccggatt
     3301 tacctgcagg cagggtggac atcttcggag ccttactcct atccactcct tcgagcttcg
     3361 ccggaatggc atttttgaca agcaaagggc aaaatttttc cactccatcc accgtatcat
     3421 aacactcata catcaccaga aacggagcat ccgcggaagt tatgattgtg agagtgtcca
     3481 tatccttcat ttttggaaaa taatccctga gaataactat ggtcgtatcc tgttccacta
     3541 tgcagggggt catattgccc agatgttctg cataatcaaa cgaaaactct ttttgcgagc
     3601 aaccgctgag gaggtagatg gcaggtagta ttgccagtac cagtttgcgg agaatgaaaa
     3661 aatcaggttt catatattaa tatttaaagt tcaaaaatta ctgatgagta tccttccatc
     3721 ttcagttgga aattttcctt cactttcttc gtctgtacca atccgttggc aaagaggggt
     3781 ttggtgagcg attcgcttag ggataacgtt atttctcctt gtccgaggtt gtgaagtaca
     3841 aggagtttct gtttcccctc tatcatatac caggcagcta cttgggagta gatacctccg
     3901 gtctgaggat tgattacggg atggcgctcc atctttcctg tagcaagtgc aggacaggaa
     3961 ttacggagtc ttcccaactg gcggtacatc gtcaggatcg aagatgcatc cgcctcctgt
     4021 gacacgacgc tacctaccgt tgaccctact gcaggatcaa ttttatcggt ataagaggtg
     4081 acgtaacgat caccccaata catcggggtc cgtacatact cgtcgccacc ggttttggtt
     4141 ccggtataac ccagttcctc tccgtaatag atatatggag agccctgcga ggagagaagc
     4201 atggctcctg ccagtttagc cttggctaat gagcctccca gttcgctgcg ggtcctgttt
     4261 tcgtcatgat tggagagttt tgtggctgca atgaagtcac tgcgttggtt gctgtaggct
     4321 ttatggatgg tgattgtggc atctgcaaat ccaagtcctt gaccttgatt tatggcggcg
     4381 ttcattcgcc accagaatga gaagtcaaat agggccggaa gccccttgta ataaggtgct
     4441 accttatccc aatcggagaa gacctctccc accatataaa tatctctgcc atttttcgct
     4501 ttgaaatggg tattgacgat atcgtaaaat tttttgagga attgagggtt ctcgtcggag
     4561 ttttcgttat gatagatatg ttttaccgca tcgagtcgaa agccgtcaac gcccgtattt
     4621 atccatccct gcgcaatcgc aactatttcc ttgaatgcaa gagaaaactc tgcagtagcc
     4681 accggcccat aattgagatc ggcaaaccag tccgtccaaa aatgagagtg gtagtaatag
     4741 atattgcccc cgggcagagt caggttcagt atgttatcgt tactcgagtt atcggtatag
     4801 agagtaaatg gcacatcaaa cgacaattgg ccggatgtcc ccttcttcgc accatatttt
     4861 gtgccgagtg gccaggatga ggagtttgaa gtccttatca ggaaacccca actggagtta
     4921 aaatctaccg tcaactcgta tacgccgtta cctttggagt aaaatttctt acaaatgcca
     4981 tcaccatagt agagatattt tgccccttct gtagtttggt cgggattatc tttatcaggc
     5041 gtaccgccac tcgaaatcgt gacggtagga gcattgccgt tgctccagtt gagagtgaat
     5101 ttataaatgc cggaaggcat cgtacctgta gaaaaccact gtccgctgtc atatcccccg
     5161 gcaccctcgg tagcaatcat agggatttta cccgccgcta tatccttttg cgggtcgttg
     5221 gaaaagatgt agtaatccct gtacgtattt ttcatcgatg acgtagctat tttgaaccat
     5281 atatggttga cacccgaatg gtttataaca tagtcgagat agatatgaat attgcgttta
     5341 tgagcctcgc tgaccagttg tttgaagtca gccatggtac cgaaacgcgg attgatcacc
     5401 ccatacgctt ctacgtcgta tccatgatag gaagcactcg gatgtattgg tgaaagccat
     5461 atcgccgagg ccccaagtga ctgaatatag tccaacttct ccgtgatgcc gcgtaaatct
     5521 ccccatccgt caccatcgga atcggcaaaa ctgtaaacca gcaattgata ggtaatatct
     5581 cctcgctttg ttccatccca ttgtggcgga tatgccatta cctccttaaa ttcctgcata
     5641 tcagggttga cggtaggatt gcaggaggag ccggtagcaa atagggttag cgtcagcgcc
     5701 agaaagaata taatttttct tctcatctcg tatttttatc gtgtctgtcc gggtattaaa
     5761 tcactaattc taccgaattg aatttcacat cattataaac tataaagata atcattccat
     5821 acgaacaatc aaaaatgcgc cgctacaagc gacgcatttt ttgttaatat agtactacac
     5881 ttatgctatt ttgtaacaac atatttcgca gtattggcaa tgtcgagagt gactttatat
     5941 gtgccggcct ctgcaacctt gatgttgcct ccgttgaaaa caagtccttt atcaccgcta
     6001 ccgagattat aatcccaatt attattgaat ttgaacttaa attcggagcc cgccttgaac
     6061 tctacagtgg cactgtaagt tctggttgcg gcatcaaatg ccatcgccac gccatctccc
     6121 cagtcatcgc ctgcggcaaa tgcacctact atggcaaccc tggtcaacgg agttgcaaca
     6181 gcctttttgg caggcaagtc tacagtccag aaataggttc catcggcgac ttccatatta
     6241 tctccactcc agagattgaa ctcgtaggtt gtacccgcta caagttggcc ggagatccag
     6301 cctgcacctg cttcatggaa cttgaattgg ttagaaccgc catacatcgc tatggcaccc
     6361 ttgtagagcc cctcagtacc tgcctgatag agagccacat catccgtagg attccaggag
     6421 tgacccgagt aactgccggg tacggtaacg tatgaagcat aagcgggagc cggagcatct
     6481 cctgtcctgg tgataactgc cctgaaaggc ttttcagaga gaatcaactc tatatcgtat
     6541 gttccacctt cggcatcttt gagatttgct ccaccataaa ccaagttagc aggctcacca
     6601 ccgaggttgt aggcaccggg tagatcccag cctgcattca tacggaactt gtactccgta
     6661 tttgcaggga acaccagacc tgaaatcttc cagctgttag aggcggcatc gtatgtcatg
     6721 gccgcatcct caggactacc ccagccgcag aatgaaccaa ccaccgcaac ccttgtaata
     6781 agaagtgccg tagcctttag ttccgtcaga tccactgaga gatagtaata acccttaggc
     6841 agattgaaat tgctgccctt caatacgagt gcatcgaagg taccgccgaa gtcattgtcc
     6901 caattgcgct cgtgggtaaa tttaaattca ccgtcaaggt gagcaaagcc ctcatagtgg
     6961 ccttctccgt cacctgtaag cacaggagct gtaccgggat tccatccctg gtggttgccg
     7021 gggatataaa gattgtccgg atatgcagta ttgataaacg tcaccgtata aggagtcttg
     7081 gagacattga ggacaatctt gtaattgcct tcgggaccct cataattgag gttattacct
     7141 atcattgagg agacattgtt cagaccggta ccaccgatag agtgtgtcca gtcatcattg
     7201 aaacggaact tgaactctcc ggggacaata tcgactgggg cgctgaaagt atgcttctct
     7261 gcatcgtata caaggttgat gtcggaactc catccaccca ctgccgtacc gataactccg
     7321 agagagccga tctgtaccat atatatggtg ttcagctttt tattcagagc aatattatag
     7381 atgcctgaag gcaccttaat attatcaggg gcaccaacgg cacctgttgc ggaggcatat
     7441 tctccggcga aggtagaaac cttaacaatg ccgccaaaat catcacccca cgcaggcttg
     7501 atgcagaact taaattcaac atccgagcca tcttcggttc tgaggtcaat aacaccctca
     7561 tagaagccct tggcaatagt cgattcgtac atcatcggag cagttgcggg attccagccc
     7621 tggtgactgc cggggagata gaggatgtcc ggatatgacg ctatataggt tttgacggga
     7681 attgtgatca cagccgaagg cacaccggaa gcataagttt ccgagaatgc ctttacgaag
     7741 aattttactt ctgaagttgc atcctcgggt gctcccgtag agacaatcgc atcattaaaa
     7801 gcatctaccg tggtggaata ggaggtctct ttaagctcgg aagccagttc aacagcatct
     7861 ccatcttcaa actgagcgta gacattgtaa gtaatctctt cattgtaacc aagacgagcc
     7921 ggctcccagg tgaagagcat gatattacct gtagggtcgg cgggatcaag tgtaaccgat
     7981 tttataactt caccgaatac gggaaccact gcatcaccga aggcatatat atcgatggtg
     8041 agggtttctg acttgtaggt attggcacca tccgtcacct gcacaaagaa cgacatcgta
     8101 aaggtattgt tctcgggaag tgaagagaag gtattataaa ggattgtctt aaaatcgctt
     8161 ttggaggcaa tgtagtaaag agtttttgtg gatgcaagtt ttgctgcagt accctcgtac
     8221 tccgcgtaga agtcatagtt caacccttcc gggaggaatc tgtatgccga ccaggagaag
     8281 ttgatatctt catccattgt attggatgtc ataaggatat ccgaatggga atagagctcc
     8341 ggagctacgg gagccgtgga gaatgcatcc tccgggaata cttcacagga ggtaatgccc
     8401 aacatcaaag cagcaactgc aataaagctt aatatctttt tcatcgtatt atcgttttaa
     8461 aggttactgt ttttccatta ctaatacata aggagtcctg ttgccgtaca atttcataat
     8521 gtaagtgccg gcagaaggta caaaaatatc accaccaccc tgaacgagct tgatgccgcc
     8581 gggaacatcc gaactggcct cgccatttgt gccgaacttg aatgtccagt tattggggtc
     8641 gtgtgcagta ttgaggcgga taaggaagcc cagtccgtca ctctccttga atgtcacggc
     8701 aggtgaaacc caaacatttt ccgttgcatc atatttcaga gggtcatcac cggtagtggc
     8761 ataggaccat ccgttataag caccaaccat tccaactcca tcaaccactt tcttatcaat
     8821 agtcattgcg ctgcgattga ctgtgagttc atagatggag ccatccctca aatcaaccga
     8881 aaggttggag tcggactgct cggggcaact ccatgaagga gtaagatagt tgtaacccca
     8941 gttagcgtca gaccagttgc gtgcaaccgt cactttaaaa taggaataat cggcactgcc
     9001 atcctgtagg tcgacaagaa tcttgtaaag gttggtgcca ccgtcagtct cccagaattg
     9061 gggggcttgg gtgatgttcc atgactgata attgccgggg aggaagagat acctcagatc
     9121 agctgcataa gtttttatat tgaaactgat gctattggat ttgacaggtg ccacaacagt
     9181 accataaatg gaagccgtga ggctggcgct gatattggcg gtctcattag gctttacctt
     9241 aaggtcgttg catacaagtc cgttcaaatc ctgcttgctt atggtaaagg agtttgaaaa
     9301 agaggtaccc gccaaaacag tatattctcc aagagtaaga tggagagcat actggatctg
     9361 agtaggtact ccaaacgaag ctgatgtcca gttgaaaact actgcctcta ccttgctgtt
     9421 gtccttattg acaataatgt ccttctgggc aagcaaagtc ggagcaacgc tttgtgcagg
     9481 atcgaccatt acatgttcta tctccatttc gcaagcggag aagaggaaca gggccatcat
     9541 cagcattact atatatttaa actgtttcat aatcttattg atttttcaat gtttcttgcc
     9601 attagtaacc gggattctgc accagcttgg ggttggcgtt gaggtcactg ataattatag
     9661 gatatacggt gcggtaatcg ggaagagcaa tcttaccgtt aggtacacca cccttcaatg
     9721 tccaggggaa ctgcattgaa gtgaagtagc cgtagcgaat gaggtctaca cgacggtggc
     9781 cctcccacat aagctctctg gcacgctcgt cgaggagcca attgaggttc attattgaag
     9841 gggttgtagc atcgacaccc gcacgttgac ggagttgacg aatataacct ttcgctgtct
     9901 cgtcggttac atttccgccg ttaaggcgtg catcagcctc ggcataaatg aggtacatct
     9961 caggaagacg gaaaacgggg aagtcaatcg aggagaattt gtagttagtc tcggcgctcg
    10021 aaaccgcatg accgtccgaa tagagtccgg agaacttcca gcatctccaa cccgaatcag
    10081 tagtggtgtt gtcaaactcc ttggtagctc cgccattgaa gaagaacgcc ctcttgtcgc
    10141 tggcagccct gtcataacca aagccattcg taccccactt cacatttacc agttcaaaac
    10201 gtgcaacata atcatctgct acgtgataac cgttccagtt ctcagggttg ataagttgac
    10261 cttctccaac aacagctgca ccgatagccg cattagcatc accgctgagt gttgccgaaa
    10321 caaggtgagt agtgcctccc cagctctgtg tctggtcctt gtcataggag attgcaaaga
    10381 taaactcctc tgctgcaccg ttagtggtgt tgtcctgcat aaagagttca tggtagttgt
    10441 cgtgcagttt gtaccccata ccgataacct tagctgccgc atctttggca tcttgccaac
    10501 gtgccgtacc tgtgtatacc tcagcattga ggtacatcct tgcaagaagt gcccaaaccg
    10561 aacccttggt tgcgcgggga taaggcacag tacccttgtc aggcatatcg gcaagtgccg
    10621 acagctcttt aagctcaccc tcaatccact tgaaaagttc agtcctgccg atttgctcgg
    10681 gcaggtcgcc gccaatgtgt tcgggcattg cgaatggagg atttccgaag aagtccataa
    10741 gcaggtaata gtatagtgca cgattgaatc ttgcctctgc acggtattgg tcaatagtct
    10801 tttggaacgc ctcatgtccg cggcttgcaa gcatctcggg agttgttctc acgaggaact
    10861 cgtttaccag ggtaatgccc ttcatgcagc gtgtgtaaac tgcgatggtg gcttcattgt
    10921 cggctgacgt ccatttatga tactggatag gattgatata gttgtcaccc cagataatct
    10981 tgagtgcatc ggtgctgagc tcattaagta tcatatacat acgagcaagc tcgctctgac
    11041 cggcgtctga cacgttaatg tccgacgatc cggcatcatt ctgacttgcc agcgcccagt
    11101 acccatagat atatcccaaa gacttgagat agctcacctc gtccttatat gcggtctccg
    11161 aggttgcgtc agtttcatta aggggctgag tatccagatc acctatgcag gaagtagcaa
    11221 ggatcataga ggccaacagc gctaagaaac ttttatataa tatcttattc atacgtctgg
    11281 tcatttttag aagttaatat ttaaacgcaa agtaaagaga cggggacggg gaatgatatt
    11341 ggaatccaca ccgtcagatg cctgcaattc gggatcgaga cctctgtact ttgaaataac
    11401 aaatacattc tgtacagaag cggagagacg gatgctctct acccatttca ctttgtcgaa
    11461 tgtgtagccg aggttaatgt cgtccatcct gaagaatgaa ccattctcga taaacatatc
    11521 ggtatatttc tggttatttt ccatagcggc agtccagccg tcaaccagat agatgttgct
    11581 taggttgtca aggtagccct tgttccagtt gtcgctgtat gagcttgcat aggacatcgc
    11641 aaccctgttg ataatgtagt taccaaagga gccgtggccg ttaaagccga agtcccattt
    11701 cttatatctg aactttagat taagtccgaa ataggcatcg ggagcgggct tgaagcctgt
    11761 cacatacctg tcagcatcag taattactcc atcgccgtca cgatccacca atccattctg
    11821 gataggattg tcctgcgaat catagagctg ctggaagagg taaaatgtaa aaggtgcaaa
    11881 tccggtgcgg tgaagtgaag tgtatccgcc cgtaccgccc ataccggaac cggtagtaac
    11941 accgagatag ccatccgtct caacacttgt aagcttggtg attttcgtac tctggagagt
    12001 aacgtttccg ccgattgaga gcgacatatc gcttgtctcg acaggaatta cattgagaga
    12061 gagctctaca cccttattct ccatattacc gatattggag ataatgctgt tgccgaagtt
    12121 tgaaccgaga ggagttgaga tttcgttgag caggtcataa gtgtagcggt aataaccttc
    12181 aaggctacct gttatcctgt cattcataaa tccgaaatcg agaccgaggt tataggtctc
    12241 ggtagtctcc catttgagat tcgggttgta cttctgggga gcgagtgtgg tataatatcc
    12301 ggtaccaaga tagtatttca tagcaatcga gctggactcc tcatatctag ccatataagg
    12361 atagtagtcc gatcctatgt cttgctgtcc ggtgcgtccc cagccaagac ggagttttag
    12421 ggctgaaacc gcatcactgc cctttaggaa gctctcttca gccacattcc aggcaaaagc
    12481 caccgaaggg aagaagcccc agcgattagc tttgctaaag cgagaggagg catcctcacg
    12541 tattgtagct gtaaagagat acctgccgag tagagagtaa tttattcttc cgaagaatga
    12601 tatcaggtaa tactccttag cattggtagg gtttgcctga tagagaggcc tagcctcgtt
    12661 attgagataa gtactctgat cataccttac atagttgtgc tgccatgagt aaccggccat
    12721 cacatccagg ttgctcttgc ccaattcctt cttataattg aagtaagcct cgagcagtgt
    12781 attggagttg tagttggcat aatcagtgta aaggtcaagg cttgagcgca tagctgaaat
    12841 tgaaccgggg tggttaaatt tttcaccgtt tgacttagcg atgtcataac cgaggtttag
    12901 gttgaatctc aaatcttcaa atccatggaa catataatca acctgtaggt taccgatcga
    12961 gcggagacct ttgttgacat ccgtgaagtc gtagagactg gaaagggggt ttgtgcctgc
    13021 catggtattg gcacttccat ctgcgttgag ccagttccag tagccgtttg ccactttaga
    13081 atagtcaata cttccgtcag catttgtaaa atgtgtcggt tttgtggggt cgaacgagag
    13141 ggcactgcct actgcgcctg tcggagccca atttgtcttt tggtagatac ccttcacatt
    13201 gagattgatg gaaagatgat cgtcaaagaa tttgggagaa aggctgatat ctacgttggc
    13261 cttctggttg tcacccactt tgagtgttgc ctgatcatag ttgtatccaa ggcttacacg
    13321 ataaggaaga tgctttgttg ctccataaac acttacagcc tggtcggtat tgaatgcgag
    13381 gcggtagatc tgcttctgcc agtccgtgct ttcattaccg agaagagtct gaatggtgtt
    13441 gttgtttgca taagtactct gcatgaagtt gcggaatgta tcggcatcca tcatgtcaat
    13501 cgtagaggca ttctgtttta cggagtaact cgaattatag ctcacgttaa ctccacgcgc
    13561 acctgcacga cccttctttg tggtaatgat aatcacaccg ttggaagcac gcgaaccata
    13621 aattgcggta gcggaagcat ccttcaatac ggtataactc tcgatgtcat tggggttaac
    13681 ggatgccagg gggttaccca ttccggcacc tccgtcagaa gtaaccggta ctccatctat
    13741 cacgatcagg ggatcgttgg atgcatttag cgaagctgca ccacgaatac ggatggtagc
    13801 ggatgcaccc ggctggcctg atgccggagt cacgtgcaga cccgatactt taccgaggag
    13861 catctgatcg ggagaagtga tggctccgcg gttgatctca tcagctttga tggctacaac
    13921 cgatcctgtc atatcgcttt ttttgacagt accatatccg ataaccacta cctcctcaag
    13981 aaattcgacg tcgtcctgca tcacaatcct tgcagctgac gcctgagcgg cggtaatggt
    14041 cacggtcttg taacccaaaa aactgatttc caacatggtc tcaccggaag gtactgtaag
    14101 gacaaaatcg ccgtccagac ccgtctgggt accgtttgca gttcccactt ggataactgc
    14161 agcaccgata atggtttcgc cttttgaatc caccacgact cccttcgcgg tgtattgcgc
    14221 aaaggcagct gtaacggaga gaaccattac cgatagtaac attgtcatta atcgtttcat
    14281 aggaaaacct ttataataat attattgttt aattgttttt ctttgaaaaa gctgtacggg
    14341 atcatccttg tcatcgacaa agaatacact tatcgcagct atcaaaagag atattccgga
    14401 aaaaaggaga gcataaatag tctgtcctcc atagatatgt tttaccatca atccccctat
    14461 cagaccattg caaatctgag ggatggtgat gaaaaagttg aatattccca tatagactcc
    14521 catcttcttt ggagggagtg atcctgccag catagcataa ggcatagcca ggattgaacc
    14581 ccaggcaata ccgataccca ccattgaaat gattagcatg gtcggattct taaaaaacag
    14641 gaacgaagca aaaccgaggg cccccaatat aagtgaaatt gcgtgtgtgg ccttacgacc
    14701 gattttgtcc gcagttacgg gaagaagaaa tgctataata gcagcaacac cgttatagat
    14761 agcctgaagt actcctaccc agtcacccgc ctcaccataa gcagcagagg cggtatccac
    14821 agttccgtag tagtggtctg caaccgcagg agtcatgtat acccacatgg agaagagggc
    14881 aaaccaggag aaaaattgca ctaaaccaag ctggcgcatg gttttgggca ttttggcaaa
    14941 atccctgaaa atctcgataa atccgccatc tttctctttc ttggctcccg gctcgggtgg
    15001 atactcttta gttcgcacca cggtcaccat aaccgaagcg aataacacaa tccctccgat
    15061 atagaaagcc aacttgacat tgggtgccac accgcccgca ctctcaggta cattagacat
    15121 gccaaaccag ttggtaagca cgaagggcag gacagcccca cagactgcac cgataccgat
    15181 gaggaaagtc tggactgaaa aaccgagcgt tctctgttct gaaggaagat tgtcagcaac
    15241 aagcgccctg aatggctcca tagctacatt gatggaggca tccataatca tcaggacacc
    15301 cgctgcaatc agaagtggcg aaagaaatgg agcaagggcc ggggagttgg gcatcagaaa
    15361 tagcatcagc gaggccaaaa ttgcacccac aagaaaatag gggcgtctac ggccaagcct
    15421 tgtccaggtc ctgtcactat agtgtccgat tatgggctgg acaatcatac ctgtcaaagg
    15481 cgcagcaagc cagaagagcg agagtgtact gacatccgct ccaaaggatt ggaaaatcct
    15541 gctgacgttt gcattctgaa gagccaaacc gaattgtatc cccaaaaagc cgaagcacat
    15601 gttgaagata ccccaaaagc tcatctttgg tcttttcata aatatatttt tgtatcagtt
    15661 tataattaaa gggactcgaa tttataaaaa aacacctcta tataatagag tttccttccg
    15721 aattgcaata aaatcgggat gaa
//
LOCUS       DAOSIE010000006        14866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_4160_length_14866_cov_4.007427, whole genome shotgun sequence.
ACCESSION   DAOSIE010000006 DAOSIE010000000
VERSION     DAOSIE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14866
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4160_length_14866_cov_4.007427"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLI69_00525"
     CDS             <1..400
                     /locus_tag="PLI69_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG41007.1"
                     /translation="DGELAGFSYIETWGHQKFVANSGLIVAHKFRQTGLARRIKQKIF
                     DLSRTLYPNAKIFSITTGLAVMKINYDLGFHPVTFSELTDDEEFWKGCSGCRNFDILQ
                     RNDYKMCLCTGLLYDPAQHSGDPKKQTENN"
     gene            406..1605
                     /gene="argG"
                     /locus_tag="PLI69_00530"
     CDS             406..1605
                     /gene="argG"
                     /locus_tag="PLI69_00530"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOG41008.1"
                     /translation="MNKKVVLAFSGGLDTSFCIPYLKNEKNFEVHTVTVNTGGFSEEE
                     AKNIEKRALELGAASHTTVDATETYYRKGIRYLIFGNILKNNTYPLSVSSERIFQALE
                     VLKQVKKIGADAVAHGSTGAGNDQVRFDIVFEILSPEVEIIAPIRKLGLSRKEEIDYL
                     QKHGFNFSWEKSRYSINQGLWGTSIGGVETLSSHGYLPEEAYPVQVTRDGSEIVTITF
                     EKGEPVALNSEKMTPVELIRKLREIAAPYGIGRDTHVGDTIIGIKGRVAFEAAAPLII
                     IKAHHLLEKHVLTKSQLYWKEQLSGFYGQMMHEAQYLDQVMRNIEVYLEDSQRAVTGE
                     VQVRLQPYNYQVLGCKSDFDLMNSKFGDYGEANKSFTGRDVIGFTKVLANPLKIYYSI
                     HDEENIL"
     gene            1619..2590
                     /gene="argC"
                     /locus_tag="PLI69_00535"
     CDS             1619..2590
                     /gene="argC"
                     /locus_tag="PLI69_00535"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOG41009.1"
                     /translation="MGKIKATIAGGTGYTAGELIRILINHPEVEITNVMSTTQVGDMV
                     SSVHRDLFGECDLKFTDKVGDPEVLFLCLGHGISREFLSTHSIPESCRIIDLGNDFRI
                     DGDYMGRDFVYGLCEMNRELIKGSYSVANPGCFATAIELGIIPLLEKKMVYDEIHVHA
                     ITGSTGAGKTPVETTHFSYRDNNISVYKSFRHQHLAEISRVIRTLTGKDIRVNFVPMR
                     GNFTRGIFASIYTRCALPAEKISAIYKAYYKSSPFVFVSDTPISLKEVVNTNKGLIHI
                     ASYDGYLHITSIIDNLVKGASGQAVQNMNLMFGYDESSGLRLKATTF"
     gene            2602..3741
                     /locus_tag="PLI69_00540"
     CDS             2602..3741
                     /locus_tag="PLI69_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG41010.1"
                     /translation="MKLFDVYPLWPIEPVKAYGCRIIDKEGKEYIDLYGGHAVISIGH
                     CHPTYVEMISGQLHKIGFYSNAVQNSLQQQLAQELGKLSDYGEFSLFLCNSGAEANEN
                     AIKLASFHTGKARVLALRKAFHGRTSGAVAATDNLAIQAPFNRTGNVTFIPMGDLAAA
                     GAEMAKGEYAAILIEGIQGVAGIFEPKADYLEGIRELCDKYEVVLIFDEVQSGYGRSG
                     RFFAHQHSGVRGDIITVAKGMGNGFPIGGVLISPLFQALHGMLGTTFGGNHLACTAAL
                     AVLKVIREESLMENAAAMGDYITSCLKGNRAIKEIRGKGLMIGIELKEGYETLRNRLL
                     FEKQIFTGGAGKNIIRLLPPLNVDKQTIDIFVEAFNQLTDDNNKQ"
     gene            3749..4705
                     /locus_tag="PLI69_00545"
     CDS             3749..4705
                     /locus_tag="PLI69_00545"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HOG41011.1"
                     /translation="MKHFTSIHDIASIDDALAKAKYVKANPLADIELGRNKTLLMIFF
                     DSSLRTRLSTQRAALNLGMNVIVLDINQGAWKMETERGVVMDGDKPEHILEAIPVMGS
                     YCDMIGVRSFARFESKEDDYKEKILNQFVKYSGKPVFSMEAATRHPLQTFADLITIEE
                     YKRTERPRVVLTWAPHPKALPQAVSNSFAEWINAKGYELVITHPKGYELADEFIGKAT
                     VEYDQEKAFEGADFIYAKNWSAYRNPNYGKVLHMDHSWCVDSRKMSLTNDAYFMHCLP
                     VRRNMIVTDDVIESPNSIVIPQAANRVVSAQVIIKELLLGIQ"
     gene            4702..5478
                     /gene="argB"
                     /locus_tag="PLI69_00550"
     CDS             4702..5478
                     /gene="argB"
                     /locus_tag="PLI69_00550"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOG41012.1"
                     /translation="MKTLQVIKIGGNVVDDPAALKRFLYDFSKIKGAKILIHGRGVMA
                     TEMAERLGIETRMYKGRRITDYETLKTVAMVYAGWINKSIVAALQSRGCNAIGLSGAD
                     CNIVPATRRSPEPIDFGYVGDVDPSNIGSEELSTLLERGLCPVFCAITHDGKGSLLNT
                     NADTMASTLAIAMTGRYDVRLIFCLEKDGVLLDRHDESSVVPRITEEYFAKLLEEGII
                     EGGMIPKLENAFAAIGNGVSEVVIKHARNLNNERGTTLIK"
     gene            5485..6564
                     /locus_tag="PLI69_00555"
     CDS             5485..6564
                     /locus_tag="PLI69_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG41013.1"
                     /translation="MESIGRYCEEAILFLEKMITIPSLSFEEANRAEFIVEYLRKRSL
                     EPQLVGNNIIVRLPHSSNDRPVLMLNSHIDTIKPSELYTFDPFNPPLDKERILGVGSN
                     DAGGSLSALLHIFIYFAKHKEHLESLKVEPLLLLTAEEERSGPDGMQRISAEMPDLAD
                     MAIVGEPTGMRAAIAERGLLVIDATAKGVSGHAARNDGDNAILIALNDISILRDFRFD
                     KISPTMGATHINVTQINGGTAHNVIPDECTFVLDIRPTDAYSNPEIMEILQNNLHSTL
                     KARNLTNRSSATPLGHPLLEAAEICSIEQYVSPTTSDWMRLDIPAIKMGPGESSRSHK
                     ADEYILKEEICRAIHTYIKFITHLK"
     gene            6561..7877
                     /gene="argH"
                     /locus_tag="PLI69_00560"
     CDS             6561..7877
                     /gene="argH"
                     /locus_tag="PLI69_00560"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOG41014.1"
                     /translation="MSTLWNKGTSATEIVEKFTVGNDKILDLRLAKYDIIGSKAHIAM
                     LATIGLLEKGEEIILTQELDRILAEIERGEFSIEDGVEDIHSQVELLLTRRVGDLGKK
                     IHSGRSRNDQVLVDLKLYLKDEAKAVRDEVHSLFDTLLKLSTRFADVLLPGYTHGQIA
                     MPSSFGMWLGAYAETLVDDMTLLAAAYRVIDQNPLGSAAGYGSSFPLDREQTTHLLGF
                     KTLHFNSIAAQMSRGKCEKAFSNALAAIASTLNKLASDCCQYMSGNYGFISFPDELTT
                     GSSIMPHKKNPDVWELIRAKSNRLQSVPNEISLLATNMPHGYHRDFQLLKDILFPAIE
                     TTHSIIRMSQYMLENMQVNKNILDDPKYDYLFTVEEVNKRVLSGVPFRDAYKSVGLEV
                     NNHTFATEISKGYDSVEKLNHTHIGSIGNLSNERIAAKMDDILKEF"
     gene            complement(7874..9421)
                     /locus_tag="PLI69_00565"
     CDS             complement(7874..9421)
                     /locus_tag="PLI69_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOG41015.1"
                     /translation="MERDLIIDVKQTEITIALMENHRLMELNKEAHGGKSYNVGDVYL
                     GKVKKLLPALNAAFVDIGDEKDAFLHYLDLGFNFKAFDFFTRQINTNKNFKSFYSGVR
                     IDEILEKEGKIERFLSPGQPIVVQIVKEPISTKGSRLTAEISIAGRNMVLLPFADKVS
                     VSQKITSKEEKRRLERLVYSILPRNYGVIIRTAAEGKKAAVLDAELRSLIKKWEDSWQ
                     KIAKSKSTQLLFTENSRTTTILRDLLNDSFSNIYVNDETIYEEVCEYVSLIAPEQEKI
                     VKLYKGSEPIFDYFEVTRQIKAAFGKIVSLRQGAYLIIEHTEALHVIDVNSGIRTKNK
                     EQENNSFDVNINAADEIARQIRLRDMGGIVVIDFIDMDANEHKNLLYKHMCEVMASDR
                     AKHTILPLTRFGLMQITRQRVRPTKEINTSEVCPTCTGTGKIGPTLLIDETLERELAF
                     YVKEKKIKEFILRTSPILAAYLTKGLLSIRYKWSRKYGCTIKVVGDTDYSIIQTRWLD
                     RKGDPLD"
     gene            complement(9635..9916)
                     /locus_tag="PLI69_00570"
     CDS             complement(9635..9916)
                     /locus_tag="PLI69_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG41016.1"
                     /translation="MTKADIVNEVAKATGLEKIAVQKVIECFMDSVKNSLEKGDNVYL
                     RGFGSFIVKERAEKTARNISKNTTIVIPAHKIPSFKPAKVFVDKVKNAQ"
     gene            10107..12521
                     /gene="lon"
                     /locus_tag="PLI69_00575"
     CDS             10107..12521
                     /gene="lon"
                     /locus_tag="PLI69_00575"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOG41017.1"
                     /translation="MKDIFDNIFSANSPDVNIIPVMNIDNIQNLKESEMPPVLPILTL
                     RNAILFPETVIPVTIGREKSIRLINESYKSNRLIGVVTQIDAKVEDPQPSDLFEVGTL
                     GKIIKIIEMPDKTITAIVQGLRRFKIDQINITAPYLMAQVTYLEDLVPDKDDKDMDAI
                     TDSIKDAALHIIRLSPNLPSEAGFAIKNLEGYEFLINFIASSIEIDNIVDKVELLRLS
                     SLKDRALKLLELLNRQIEILKIKDDIQRKVKSEIDQQQREYYLSNQLKTIQEELGIGS
                     EQHDFSQLREKAANKKWPQYAADAFEKELKKLENTNPSSPDFNIQFSYVEFLTDLPWD
                     EVTEDDLDMKNAQKILDEDHYGLEKVKERIIEYLAVLKLKGDMKSPIICLHGPPGVGK
                     TSLGKSVARTLGRKYGRISLGGLHDESEIRGHRRTYIGAMPGRIIQTIKRAGSSNPVI
                     VLDEIDKVGDNFRGDPASALLEVLDPEQNTAFHDNYLDIDYDLSRVLFITTANNVGAI
                     HPALRDRMELINVSGYLAQEKVSIAEGYLIPKQIEAHGLKKEDMQLSREAIDTIIDYY
                     TRESGVRGLDKQIAKVARITAKKIALEEELKPLLGSDDIKEYLGLPTNQPNIQEGNEA
                     PGVVTGLAWTEMGGDILFVECSLSEGKGVLSTTGNLGDVMKESATIALQYLKAHPELS
                     GVDGKSFYKKDFHIHVPEGAIPKDGPSAGITMVTAIASALQGKSIRSGIAMTGEITLR
                     GKVLPVGGIKEKILAAKRAGVKTIVLSHENEKDIKEIKEIYIEGLEFKYVKTIDEVIN
                     YVFSNK"
     gene            12524..13192
                     /gene="ung"
                     /locus_tag="PLI69_00580"
     CDS             12524..13192
                     /gene="ung"
                     /locus_tag="PLI69_00580"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018910762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOG41018.1"
                     /translation="MDVKIESSWKEALNEEFKKPYFASLVETLRDEKRQGRIIYPPGP
                     QIFHAFDLTPIDKVKVVILGQDPYHGPGQAHGLSFSVPKGVQPPPSLLNIYKEIENDL
                     GITLNKDGSLEGWARQGVFLLNAVLTVRASEATSHSGIGWMEFTDAVIRILSQKRENI
                     VFLLWGNFARSKKELIDTSKHFVFEAAHPSPLARGAFFGCRHFSMTNEALVSRGLSPI
                     DWSL"
     gene            13255..13728
                     /gene="coaD"
                     /locus_tag="PLI69_00585"
     CDS             13255..13728
                     /gene="coaD"
                     /locus_tag="PLI69_00585"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOG41019.1"
                     /translation="MRLALFPGSFDPFTMGHLDVLKSALKLFDKVIVAVGYNSSKGGL
                     FSPEARVEMIKKAIGDMERVEVDSYTGLTVDYCRARGAVCIIRGLRTTTDFELESVIA
                     QANRKMAPEISSIFIPAGHEYSFISSTVVRDVLMNGGDAKCFLPPGIDIDDYINK"
     gene            13744..>14866
                     /locus_tag="PLI69_00590"
     CDS             13744..>14866
                     /locus_tag="PLI69_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG41020.1"
                     /translation="MKLRVISLVFVLVIGIFSTSGQNTAKLHKGIEEYFDSLIYYPID
                     TTTSRIDRLITALPDKKEQAKVAGAAFDYFYGSPIMGMEAVSLHIADNWFLNGKLEWA
                     NPESWHLLYTFAEFNRSSMIGCDAPELILESIEGYMINILKGDTRWKILYFYDDQCST
                     CKEETPGLARFAREYSGPRITIFAIYTQGDRKEWQEYVNRVFGDISNPDVTILHLWDP
                     EANSAYHMKYGVLTTPSMFLIDRFNIIAGRKLNCEALYRLLDVKFNESNKFRKLFVNI
                     FSSMEPVDEDVIKQVAETFYRRTALDSALYRETFHELYSFLKNTAGADYQQGALDIGR
                     SYMLEKEEYWSKEYLEYISYDIRLSSTNLPGEKASDLLLN"
BASE COUNT         4284 a         3396 c         3407 g         3779 t
ORIGIN      
        1 ggatggtgaa cttgccggct tttcctatat agagacctgg ggacaccaga agtttgtagc
       61 aaattccggt ctgatagtag cacacaaatt tcgacagacc ggccttgcca gacgcataaa
      121 acagaagatc ttcgaccttt ccagaactct ttacccaaat gctaagatat tcagcatcac
      181 caccggtttg gcggtaatga agattaatta cgatttggga tttcaccctg taaccttttc
      241 cgaacttacg gacgatgagg agttttggaa gggttgcagc ggatgccgca attttgatat
      301 tcttcagagg aatgattaca aaatgtgcct ttgtacaggt ctgctctatg atcctgcaca
      361 acattcgggc gatcccaaaa aacaaacgga aaacaattaa aagatatgaa taaaaaagtc
      421 gtacttgcat ttagcggagg tctggatacc tctttttgca ttccttacct caaaaatgaa
      481 aagaattttg aagttcatac ggtaactgtc aataccggcg gtttttccga agaggaagca
      541 aaaaacatcg aaaagagagc acttgaactt ggagctgctt ctcacaccac tgtggatgcc
      601 acggaaacat actaccgtaa aggcatcaga tatcttattt tcggaaatat acttaaaaac
      661 aacacttatc ccctttccgt aagttcagag cgcatattcc aggcactgga ggtactaaaa
      721 caggtcaaaa agattggtgc cgatgcggta gcgcacggca gcacgggagc aggcaacgac
      781 caggtacggt ttgatatagt attcgagatc ctgtcgcccg aagtggagat tattgccccc
      841 ataaggaaat tgggcttaag tcggaaagaa gagattgatt acctgcagaa gcacggattc
      901 aatttctcct gggagaaatc caggtactcc atcaaccaag gcctctgggg taccagtatt
      961 ggaggagtgg agactctctc ttctcacggc tacctccccg aggaggcata tccggtacag
     1021 gtgacccgcg atgggagcga aattgtgacc ataacctttg aaaagggaga gccggtggcg
     1081 ctcaattcgg agaaaatgac tcccgtggag ctgatccgta aactcaggga aattgcagct
     1141 ccctacggta tcggtcgtga tactcatgtt ggggatacaa ttataggcat caagggcaga
     1201 gtggcatttg aggcggcggc cccactgatt ataataaagg cgcaccacct actcgagaag
     1261 catgtgctga ctaagtcaca actctactgg aaagagcagc tctcgggatt ctacggccag
     1321 atgatgcatg aggcgcagta ccttgaccag gtgatgcgca atattgaagt ctatcttgag
     1381 gactcacagc gcgctgttac gggtgaagtg caggttcgtc tgcaacccta caattatcag
     1441 gttctgggct gcaagagcga tttcgacctt atgaactcca aattcggcga ttacggagag
     1501 gccaataagt cattcaccgg cagggatgta ataggattta ccaaggtatt ggccaatccg
     1561 ctcaagatct attattcgat acacgatgag gaaaatatcc tctaagaaaa attatacaat
     1621 gggaaaaatc aaagccacga tagcgggagg cactggatat accgccggtg aactgatcag
     1681 gattttgatc aaccatcccg aggttgaaat caccaatgtt atgagtacca ctcaggttgg
     1741 tgatatggtt tccagtgtgc acagagatct ttttggagag tgtgacttaa aattcaccga
     1801 taaggtgggg gatccggagg tactcttcct atgtctggga cacggcattt cacgtgaatt
     1861 tctctccacc cactccatcc cggagagttg ccgaatcata gatttaggca atgatttcag
     1921 aattgacggc gattatatgg gaagggattt tgtctatgga ctctgcgaaa tgaataggga
     1981 gttgatcaaa ggtagttatt ccgtagccaa tcccggctgc tttgcaacag ccatcgagtt
     2041 aggaataata ccgctccttg agaaaaagat ggtgtacgac gaaatccacg ttcacgccat
     2101 taccggttct accggcgcag gcaaaacacc tgtcgagacc acccatttca gctacaggga
     2161 caacaacatt tctgtttaca aatccttcag acatcaacat ctggcagaaa tctccagagt
     2221 tatccgcaca cttacgggta aagatatccg tgtcaatttc gtgccgatga gaggcaattt
     2281 tacccgaggc atatttgcca gcatctatac aaggtgtgca ctgccagcag aaaagatatc
     2341 cgcaatttac aaagcatatt acaagagttc gccttttgtc tttgtttcgg atacccccat
     2401 cagtctcaaa gaggtggtta ataccaacaa agggcttatc cacattgctt cctacgatgg
     2461 ttatctgcac attacctcta taattgacaa tctggtaaaa ggggcatccg gacaagccgt
     2521 acagaatatg aacctcatgt tcggctatga tgaaagcagc ggattgagac tcaaagccac
     2581 cacattctaa agatcaggta tatgaagctc ttcgatgttt accctctctg gcccattgaa
     2641 cctgtcaagg catacgggtg cagaattatt gacaaggaag gaaaagaata tatcgatctc
     2701 tacggaggtc acgccgttat ttccataggt cactgccatc ccacttatgt tgaaatgatc
     2761 agcgggcagc ttcataaaat aggtttctat tccaatgccg tgcaaaattc ccttcaacaa
     2821 caactggcac aagagcttgg taaactatct gactacgggg agttttcact tttcctctgc
     2881 aattccggcg cagaagccaa tgaaaatgcc ataaaactgg cctctttcca taccggaaaa
     2941 gcgagagtac tggcactacg gaaggcattc cacggccgta cttccggtgc cgtggctgcc
     3001 accgacaatc tcgcaataca ggctcctttc aaccgcacag gcaatgtcac cttcatacct
     3061 atgggcgatc ttgctgctgc cggtgccgag atggcaaagg gggaatacgc agctatcctg
     3121 attgaaggca tccaaggagt agcgggtata tttgaaccca aagcagacta cctcgaaggc
     3181 atcagggaac tttgcgacaa gtacgaagta gtgctcattt ttgacgaagt gcagtcggga
     3241 tatggaagaa gcggccgttt ttttgcccac caacatagcg gagtgagagg tgacatcatc
     3301 accgtagcca aagggatggg aaacggcttc cctattggcg gagtgcttat ctccccactc
     3361 ttccaggctc tacacggtat gctcggaacc actttcgggg gcaaccacct cgcctgtacg
     3421 gcagctttgg cggtattaaa agtaatcaga gaggaatctt tgatggaaaa tgctgccgca
     3481 atgggtgact atataactag ttgtctgaag ggaaacagag ccatcaagga aattcggggt
     3541 aaaggtctga tgatcggtat tgaattgaaa gaggggtacg agactctacg caaccgatta
     3601 ctatttgaaa aacaaatttt caccggaggt gcgggcaaaa acatcatccg tctgcttcct
     3661 cctctgaatg ttgataaaca aacaatagat atctttgtag aagcttttaa tcaactaact
     3721 gatgataaca acaaacaata ataacctcat gaaacatttt acctcaatac atgacatcgc
     3781 atccattgac gatgcgctcg ccaaggccaa atatgtcaag gctaatcccc tcgcagacat
     3841 tgaactggga cgaaacaaga ccctgcttat gatatttttc gactccagtc tccgcacaag
     3901 actcagcact cagagggcag ccctcaacct tggaatgaat gtgatagtcc tcgacatcaa
     3961 tcagggtgcc tggaaaatgg agactgaaag aggggttgtc atggatggag acaaacccga
     4021 acacatactc gaggctatac ctgtgatggg gtcttattgc gacatgatcg gagttagatc
     4081 ttttgccaga tttgagagta aagaagatga ctataaagaa aaaatactca accagtttgt
     4141 caaatattcc ggaaagccgg ttttcagcat ggaggctgcc acaagacacc cccttcaaac
     4201 atttgctgac ctcataacca ttgaggaata taagagaacc gaaaggccca gggtggtgct
     4261 aacctgggct ccacacccca aagccctgcc tcaggctgtg agtaattcat ttgccgagtg
     4321 gatcaatgcc aaagggtatg aacttgtaat tacacacccc aaaggttacg aactcgctga
     4381 tgagtttatc ggaaaagcca cggtggaata tgatcaggag aaggcatttg agggtgccga
     4441 cttcatctat gccaaaaact ggtctgcata caggaatccc aactacggca aagtattgca
     4501 tatggatcac tcctggtgcg tggacagcag gaaaatgtcc cttaccaatg atgcctattt
     4561 catgcattgt ctgcctgtaa gacgcaacat gatagtgact gatgatgtca tagagtctcc
     4621 taactccata gttatcccac aagcggccaa cagagtagtc tctgcgcagg taataataaa
     4681 agaacttctt ctcggaatcc aatgaaaact ctacaggtca taaagatagg aggcaacgtg
     4741 gtcgatgacc ccgctgcact caaacgtttc ctttatgatt tcagtaagat aaaaggagct
     4801 aaaatactca tacacggtag aggagtgatg gctacagaga tggctgaaag actcggcata
     4861 gagacccgaa tgtataaagg gcggcgtatc acggattatg agacactcaa gaccgttgcg
     4921 atggtctatg ctggatggat caacaaatcg atagttgcag ctttgcaaag caggggttgc
     4981 aatgccatcg gcctttccgg tgcagattgc aatatagtac ccgctacaag acgctcacct
     5041 gagcctatag acttcggata tgtgggagac gtcgatccct ctaatattgg cagcgaagag
     5101 ctttcgactc tgcttgagag aggtctttgc cctgttttct gtgccatcac gcacgacgga
     5161 aagggttcgc tcctcaacac taacgccgac actatggcat caacgcttgc aatagcgatg
     5221 accggcagat atgatgtccg tctgatattc tgtcttgaaa aggatggagt actcctggac
     5281 cggcatgatg agtcctcagt tgtcccaagg ataacggagg aatatttcgc gaagctactt
     5341 gaagagggta taattgaagg aggaatgatt ccaaaactgg agaatgcatt tgccgccata
     5401 ggcaatggag tatccgaagt tgtgatcaaa catgctcgca acctgaataa tgagaggggt
     5461 acaaccctta taaaatagaa aatcatggag agtatcggca gatattgcga agaggcaatc
     5521 ttattcctcg aaaaaatgat taccattcct tcgctctcat tcgaagaggc gaacagggcg
     5581 gaatttatag tagaatatct caggaagagg tctctcgaac ctcaacttgt cggcaataat
     5641 attattgtac gccttcctca ttcttccaac gaccgccccg tgctaatgct caacagtcat
     5701 atagacacga tcaagccctc cgaactctac accttcgacc cattcaatcc ccctttggac
     5761 aaggagcgga tactcggggt tggaagcaat gatgccggtg gaagtctctc cgctctcctc
     5821 catatattta tctattttgc gaagcataag gaacacctgg aatccctcaa agtagagccg
     5881 ctgctgctgc ttacagctga agaggagcgc tcaggtcctg acggtatgca aagaataagc
     5941 gcagaaatgc cggatcttgc agatatggcg attgttggcg aacctacagg catgcgcgca
     6001 gctatcgctg aaagggggtt gctggtgata gatgctactg caaagggagt cagcggacac
     6061 gccgcccgca atgatggcga taatgcaatc cttatcgccc tcaatgacat ctcaattctc
     6121 agagatttcc gatttgacaa aatatctccg acaatgggag ctacccatat caacgtcaca
     6181 cagattaatg gcggtacggc gcacaatgtt attccggatg agtgtacttt tgtgctggac
     6241 atcagaccga ctgacgcata cagcaatcct gagataatgg aaatcctcca aaataatctg
     6301 cacagtactc tgaaagcacg caacctcacc aaccgatcat ctgcaacgcc cctagggcat
     6361 cccctgcttg aggctgctga gatatgctca atagagcaat atgtctcccc caccacttcc
     6421 gattggatga gactcgatat ccctgctata aaaatggggc ccggagagtc atcacgttca
     6481 cacaaggcgg acgaatatat actcaaggag gagatatgcc gagctattca cacttacata
     6541 aaattcataa cccatctgaa atgagcactc tttggaacaa aggcacatct gccacggaaa
     6601 ttgttgaaaa atttacagta ggcaatgata aaatacttga tctgcgcctc gcaaaatatg
     6661 acataatcgg ctccaaagcc cacattgcga tgctggccac cattgggctt ctcgaaaaag
     6721 gagaagagat catactcact caggagttgg acaggatcct cgccgagata gaaaggggtg
     6781 aattttcaat agaagatgga gtggaagata tccattcgca ggtggagctg cttcttaccc
     6841 gcagagtagg cgacctcggc aaaaaaatac actcgggacg ctcccgtaat gatcaggtgc
     6901 tggtggacct caaactctac ttaaaggatg aggctaaagc ggttagagat gaagttcaca
     6961 gcctttttga cacgctactc aaactaagta caaggttcgc tgacgttctt ctcccgggtt
     7021 atacacacgg acagattgca atgccctcat cattcggcat gtggcttgga gcttatgcgg
     7081 aaacattggt ggatgatatg acgctgttgg cggctgcata tagggtaatt gaccagaatc
     7141 ctctcggctc cgctgccggg tatggctcct catttccgct ggacagggaa cagaccaccc
     7201 atctgcttgg atttaaaaca cttcacttca actccattgc agcgcagatg agtcgtggca
     7261 aatgcgaaaa agcatttagt aatgccctgg ctgccatagc ctccaccctc aacaaactcg
     7321 cttctgactg ttgtcaatat atgagcggca attatggttt catttcattt cccgacgaac
     7381 ttaccacagg ctcgagcata atgcctcata agaaaaatcc cgatgtttgg gaacttatca
     7441 gagctaaaag taatcgcctg cagagcgttc caaatgagat ttctctcctc gctacaaata
     7501 tgcctcatgg ctaccacaga gatttccagt tgttgaaaga tattctcttc cccgctattg
     7561 aaaccaccca tagcataata agaatgtcgc aatatatgct ggaaaatatg caggtgaata
     7621 aaaatattct ggatgacccc aaatatgact acctgtttac ggtagaagag gtaaataaac
     7681 gcgtactatc cggtgttccc ttcagggatg cctataaaag tgtaggactt gaagtaaaca
     7741 accacacttt tgctacagag atctcaaaag gatatgatag cgttgaaaaa ctcaaccata
     7801 cccatatagg aagcataggc aatctctcaa atgagagaat tgccgctaaa atggatgata
     7861 tattgaagga gttttagtcc agaggatcgc ccttgcggtc caaccatcgg gtctggatga
     7921 tggaatagtc ggtatctcct accactttta tagtacaacc gtactttctc gaccatttat
     7981 aacgaatgct caacaatcct tttgtaagat atgctgcaag aatgggactt gtacggagta
     8041 tgaattcttt aatctttttc tcctttacat agaaagccag ctctctctcg agagtttcat
     8101 ctataaggag tgtggggccg atcttacccg tacctgtaca ggtgggacat acctccgaag
     8161 tattgatttc ttttgtagga cgaacccttt gacgggtaat ctgcatcagg ccgaatctgg
     8221 tcaggggaag gatggtatgt tttgccctgt ccgaggccat aacctcacac atgtgtttat
     8281 agagcagatt tttgtgctca ttggcgtcca tatcgataaa atcaatgacc acgatgcctc
     8341 ccatatccct caggcgaatc tggcgtgcga tctcatcagc agcattgatg ttgacgtcaa
     8401 aggaattgtt ctcctgttcc ttgtttttgg tgcggatgcc gctattaacg tcaattacat
     8461 gcagcgcttc ggtatgttct atgataaggt aggccccctg acgcaatgag actatcttgc
     8521 cgaatgccgc tttgatctgc cttgtaacct caaagtagtc aaagatgggc tccgaaccct
     8581 tatagagttt gactatcttt tcctgctccg gagcgatgag tgatacgtat tcacaaacct
     8641 cttcatagat ggtctcatca ttgacataga tattggagaa ggagtcgttc agcaagtcac
     8701 gcagtatggt ggtagtcctg ctgttctccg taaaaagtaa ttgagtggac tttgatttgg
     8761 caattttctg ccatgagtcc tcccatttct ttattagtga cctgagttcc gcatccagta
     8821 cggctgcttt cttgccctct gcggcagtcc tgatgattac accgtaattc cggggaagta
     8881 tactataaac gaggcgctca agacggcgtt tttcctcttt ggatgtaatt ttctgcgata
     8941 cgctgacctt gtcggcaaat ggcagaagta ccatattgcg tcctgcgatg gatatttccg
     9001 cagtgagtct cgaacccttt gtggaaatgg gctccttgac tatctgaacg actatgggct
     9061 gcccgggaga gagaaacctc tcaattttac cctctttttc aagaatctca tcaatccgta
     9121 ctccagagta gaatgatttg aaattcttat tggtattgat ctggcgtgta aaaaagtcga
     9181 atgccttgaa attgaatccg aggtcgagat agtgtaggaa tgcatctttc tcatccccta
     9241 tatccacaaa ggccgcattg agtgcaggta ggagcttctt gactttgccg agatacacat
     9301 cgccgacatt gtaacttttg cccccatgcg cctccttgtt gagttccata agacggtgat
     9361 tctccatcaa agcaatggta atctcagtct gcttgacatc aattatcaga tctctctcca
     9421 tcgaaaaaca gttcctttat aaaagacaat aatctgaagc gggccgcaga ttgctcgcga
     9481 aaccacttca gattatacac tgtaaggcca aaatcagcag caaattattt cttcttgtgt
     9541 ctgttcttgc gcaaacgttt tttacgtttg tgcgtggcca ttttatgtct ctttcttttc
     9601 ttaccgcttg gcatatttct attattttaa taaattattg tgcgtttttt accttgtcga
     9661 caaaaacttt tgcgggcttg aatgaaggga ttttgtgggc cgggataaca atggttgtgt
     9721 tttttgaaat gttgcgggca gtcttttctg cacgttcctt cacgatgaaa ctgccgaaac
     9781 cgcggagata cacattgtct cctttttcaa gtgaattttt aacgctatcc atgaagcact
     9841 ctatcacttt ctgtactgcg atcttttcca aaccggtagc ctttgcaacc tcgtttacaa
     9901 tgtctgcttt tgtcataaca atttagtatt taatggttca tatttcttat tggggatgca
     9961 aaattaaagt taatttttta atattcaaaa agtaatgcat ttttttccct tttttaagtt
    10021 tggcataatg attgaccata ataagggtga cacctgaaaa ggtgtgaaaa cggtttattt
    10081 atgttttatt actgacattt tgacacatga aagatatatt tgacaacatt ttctcagcaa
    10141 actcaccaga tgtaaacatt atacctgtga tgaacatcga caacatacag aatctcaaag
    10201 agagtgaaat gcctcctgtg ttaccgattc tgacactcag gaatgccatt ctatttcccg
    10261 aaactgtaat accggtaacc ataggcaggg aaaaatccat ccgtctgatt aacgaatcat
    10321 acaaatccaa tcgcttgatt ggggtggtga ctcaaattga tgcaaaagtg gaggatcccc
    10381 aaccgtcaga tctcttcgaa gtgggcactc tgggcaaaat tatcaaaatt atcgagatgc
    10441 ccgataaaac tataaccgct atagtgcaag gtttacgcag atttaagatt gatcagataa
    10501 acatcactgc accctatcta atggcgcaag tgacctatct cgaagatcta gttcccgaca
    10561 aggacgataa agatatggat gccataaccg acagtatcaa ggatgctgca ctccatatca
    10621 taaggctctc ccccaacctc ccttcggagg caggatttgc cattaaaaat ctggagggat
    10681 atgagttcct gatcaatttt atagcctcga gcatagagat tgacaacatt gtcgacaaag
    10741 tggaattgct tcgactctca tcacttaagg acagggcgct aaaattgctc gaattgctca
    10801 accgccagat tgagattctc aaaatcaagg acgacataca aaggaaagta aaaagcgaga
    10861 ttgaccagca gcagagggaa tactatctga gcaaccagct caagaccatt caggaagagc
    10921 tgggaatcgg ctccgagcag catgactttt cccagttgag ggagaaggcc gcaaataaaa
    10981 aatggcccca atatgctgcc gatgcctttg agaaagagct taagaaactc gaaaacacca
    11041 accctagttc acccgacttc aacatacagt tttcttatgt ggaattcctt acagaccttc
    11101 cttgggatga ggttacagag gacgatctgg atatgaaaaa tgcccagaaa atacttgacg
    11161 aggatcatta cggccttgaa aaggtcaagg aaagaattat agaatacctt gccgttctca
    11221 agcttaaggg tgatatgaaa tcccctataa tatgccttca tgggcctccg ggagtgggta
    11281 aaacctctct tggcaaatct gtggcgcgta cactcggcag aaaatatgga agaatctccc
    11341 tcggaggact ccatgatgag tccgagatcc gaggacatag gagaacctat atcggagcta
    11401 tgcccggccg tataatacag accattaaga gggccggcag ttccaatccg gtaatcgtgc
    11461 tggatgaaat agataaggta ggagataatt tcaggggcga ccccgcatcg gcattgctcg
    11521 aagtgctcga tccggaacag aatactgcat ttcacgacaa ctatctcgac atagattacg
    11581 atctgtcgag agtgcttttt ataactacag caaataatgt aggagccata catcccgcac
    11641 tccgtgacag gatggaactc atcaatgtct ccggctatct tgcccaagag aaagtttcca
    11701 tcgcagaagg ctacctcatc cccaagcaaa ttgaggccca cggcctgaaa aaggaggata
    11761 tgcaactttc gagagaggct atagatacaa tcatagatta ctacacccga gagtccggag
    11821 taagaggcct tgacaaacag atagccaagg tggcacgtat cacggccaag aagatagccc
    11881 tagaagagga gcttaagcct ctgcttggca gcgacgatat taaggagtat ctcggattgc
    11941 ccaccaacca acccaacatt caggaaggca atgaagcccc cggagtagtc acaggccttg
    12001 cctggactga gatgggtggt gacattctat ttgtagagtg cagcttgagt gaagggaagg
    12061 gagtactctc gaccaccggt aatctcggag atgtgatgaa ggagtctgca acaatcgcac
    12121 tacaatacct taaagctcac cctgagcttt ccggagtgga tggaaagagt ttctacaaaa
    12181 aagacttcca tatacacgta ccggaaggtg ccattccgaa agatggacca tcggcaggta
    12241 tcaccatggt cacggcaatt gcttcagcgc tgcagggcaa gagtatccgc agcggcatag
    12301 ccatgaccgg tgagatcact ctcaggggta aggtgctccc ggttggcggc atcaaggaga
    12361 agatccttgc tgccaaaagg gccggagtaa agacaatcgt actctcgcat gagaatgaaa
    12421 aggatattaa ggagatcaag gagatctaca ttgaaggcct tgagttcaaa tatgtgaaaa
    12481 ccattgacga agtaattaac tacgtctttt caaataaata gtcatggatg tgaagattga
    12541 aagcagttgg aaagaggctc tgaacgagga atttaaaaaa ccctatttcg cttctctcgt
    12601 ggagactctt cgggacgaaa agcgtcaggg aagaattatc taccctcccg ggccgcaaat
    12661 tttccatgct tttgatctca ccccaataga taaggtaaag gtggtaatcc tggggcagga
    12721 cccctatcat gggcccggac aggctcacgg cctctctttt tcagtgccta aaggagtcca
    12781 gccgccccct tccctactga atatttacaa agagatagaa aatgacctgg gcatcactct
    12841 gaataaagat ggttcgttgg agggatgggc acgccaaggg gtctttctgc tcaatgcggt
    12901 actcaccgta agggcttctg aagccacttc ccacagcggg ataggctgga tggagtttac
    12961 ggatgccgtc atccgcatcc tttcgcaaaa gcgcgaaaat attgtttttc tgctttgggg
    13021 taattttgca aggagcaaaa aggagctgat cgatacctcg aaacacttcg tcttcgaagc
    13081 ggcacaccct tcgcctctgg caaggggtgc ttttttcgga tgcaggcatt tctctatgac
    13141 caacgaggct cttgtcagcc gaggactctc tcctattgac tggagtttat aataagtttt
    13201 tgaaatattg ttttttaact ataacttcgc ggtgtaatat ctgatatgaa aaatatgaga
    13261 cttgcgcttt ttccgggttc ttttgatcca tttacaatgg gacacctgga tgtattgaaa
    13321 tccgcactga aactttttga caaagtcatt gtggcagtgg gatacaacag ttccaaaggc
    13381 ggactctttt cccctgaagc cagagtcgag atgataaaga aagccatcgg agatatggaa
    13441 cgagtggaag tagactccta tactggcctt actgttgatt attgtagagc aagaggtgcg
    13501 gtatgcatta ttagaggctt gagaaccacc accgactttg aactggaaag tgtcatagcc
    13561 caggccaacc gcaaaatggc accggagatc tccagcatct ttattcccgc gggacacgaa
    13621 tattcattta tctcctcgac agtagtaaga gatgtactca tgaatggcgg tgacgcaaaa
    13681 tgttttttgc ctcccggcat tgacattgac gattacatta ataaatagca tccaaataca
    13741 ggcatgaaac taagggtaat atctttggtt ttcgtcctgg tcatcggaat cttttccaca
    13801 tcgggccaga acacggccaa actccacaaa ggcatcgaag aatacttcga tagcctgatc
    13861 tattatccca tcgataccac aacatcgcgt attgaccgtt tgataacagc ccttcctgac
    13921 aaaaaggagc aggctaaagt tgccggagcc gcattcgact atttttacgg ctctcccata
    13981 atggggatgg aagcggtttc cctacatatt gccgacaact ggtttctcaa tgggaagctc
    14041 gaatgggcta atcccgaaag ctggcatctg ctctatactt tcgccgaatt caacagatca
    14101 tccatgatcg gatgcgatgc ccccgaactc attctcgaga gtattgaagg ctacatgata
    14161 aatattctca aaggtgacac tcggtggaaa atactctatt tctatgacga ccaatgcagt
    14221 acctgcaaag aagagactcc cgggctggcc cggtttgcaa gggagtattc gggaccgcga
    14281 atcaccatct tcgcaatcta tacgcaaggc gaccgcaaag agtggcaaga gtacgtcaac
    14341 cgcgttttcg gagatatttc caatccggat gtaaccatac ttcacctgtg ggatcccgaa
    14401 gccaacagtg cctatcacat gaaatatggt gtgcttacca caccttcgat gtttcttata
    14461 gatagattca atataattgc aggtcgtaaa ctcaactgcg aggctctata tcgccttctg
    14521 gatgtaaagt tcaacgaaag caataaattc aggaaactct ttgtcaatat attctcttcg
    14581 atggagccgg ttgacgaaga tgtcataaaa caagttgcgg agacatttta tcgtaggacg
    14641 gccttggatt cggcacttta ccgcgaaaca tttcacgaac tctattcttt tttaaaaaac
    14701 accgccggag ccgattatca gcagggtgcg cttgatattg gccgtagtta tatgcttgag
    14761 aaagaggaat actggtccaa agaatacctg gagtatatct cctacgatat aagactttcc
    14821 tccaccaatc tccccggaga gaaggcatca gaccttttgc tcaacg
//
LOCUS       DAOSIE010000007        14273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_4347_length_14273_cov_4.664791, whole genome shotgun sequence.
ACCESSION   DAOSIE010000007 DAOSIE010000000
VERSION     DAOSIE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14273
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4347_length_14273_cov_4.664791"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLI69_00595"
     CDS             <1..313
                     /locus_tag="PLI69_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41021.1"
                     /translation="LFFVRRKILKSIASFIVINMVLSILIIALIKGLDVLGFLEGYEG
                     SVGYKGDINISYGDRGLFDFYRGDHPLMIFMMILRLFMEMVLPILLMLGSYRLMKTNR
                     Y"
     gene            316..693
                     /locus_tag="PLI69_00600"
     CDS             316..693
                     /locus_tag="PLI69_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG41022.1"
                     /translation="MINFDEHKPIYLQISDSICEKILNDEYAEDERIPSIREMGAQLG
                     VNPNTVMRSYDHLKSLDIIYDKRGMGFFASPDAKRSVKKMYKKEFFEMELPAIMKKMK
                     LLDIDTDELVLKMHGTDDKHSEF"
     gene            697..1395
                     /locus_tag="PLI69_00605"
     CDS             697..1395
                     /locus_tag="PLI69_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG41023.1"
                     /translation="MIDIKQLHKSYYTEAISLHVLKGLDLKIEAGEYVSIMGASGSGK
                     STLLNIIGILDTYDEGEYRLNGTLIRDLSEKQAAKYRNEMIGFVFQSFNLINFKNALE
                     NVALPLYYKNIGRKQRNIIAMEHLDKMGLKDWAHHLPNELSGGQKQRVAIARAMISNP
                     KIILADEPTGALDSQTTIEVIGVLTDLNKQGITTIIVTHEQSVADSTNRIIRLKDGMI
                     ESDTRHPHPVPRNS"
     gene            1403..2665
                     /locus_tag="PLI69_00610"
     CDS             1403..2665
                     /locus_tag="PLI69_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG41024.1"
                     /translation="MDYLQEIFATLRKNKLRTILTGLSVSWGIFILIILLGAGNGLKN
                     GVTSNFEQRAVNRVNLYAGYTSMPYKGLKSGRSIEFTENELSAVEQELEESRMVTARS
                     STTQNISYGVEYGSYDVRGVMPSYAEMEKLIFEPGNGRFFNQLDYKQKSKVIVLDTKI
                     AEVLFKGKSPLGEYVKVGQLMFKVIGVNSKKAQWGGPVAYIPFSISQAIFNPDKKFNQ
                     LTFTVEGLESKDENELFNSSVRNLMGRRLKFDPSDEQALWVNNTQADYLETMKIFGGI
                     NVFVTIIGILTLLAGIVGVSNIMLVSVKERTREIGIRKAIGAPPASILTSIILESIII
                     TAIFGYIGMFLGIGLTELINFFMEMSASAAQSVDAGPQMSVFKNPTVNVGYAIFATII
                     LIISGVIAGYLPARKAVKIKPIEAMRQE"
     gene            2777..4027
                     /locus_tag="PLI69_00615"
     CDS             2777..4027
                     /locus_tag="PLI69_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG41025.1"
                     /translation="MLLFDIDRWQEIWITITSNKSRSFLTAFGVFWGIFMLVLMVGSG
                     NALETGIMSQMEGFATNSVFFAAERTTEPYKGFRKGRSWDMTNSDLPIIRERVDEMQY
                     MSPVLFAGGGDKNVVRGDKSGSYLVKGCYPEYDLIEKSKMLYGRYVNDIDIAEKRKVC
                     VIGERIYEVLFHGEDPTGKQIRVNGIYFQVIGVARSTSQVSIGGQTAETVVLPFTTMQ
                     QAFNQGNIIHFLAATAKPGMDVKIIEDKITNILKQQHQIAPEDKSAVFSMNIEEQFKM
                     FNNLGIGIAALIWIVGLGTLLAGAIGVSNIMLVTVRERTKEIGIRRALGATPNNIIGQ
                     IMSETVVLTLLAGIGGIVLGVGLLSAIGVALSQGDQFFKDPQISFSMAIVSLIILIVI
                     GLLAGLVPAQRAMMIKPVEAIAEE"
     gene            4124..5227
                     /locus_tag="PLI69_00620"
     CDS             4124..5227
                     /locus_tag="PLI69_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG41026.1"
                     /translation="MRKVLKIVGLVLLGVLVIGTFVFLYKKSRPVRKTYQIETVIQGT
                     IEKKTVATGKVEPRNEILIKPQMSGIVSEVYKEAGDRVLAGDIIAKIQVVPDMVNLSG
                     AESRVTRAQLAAEQSKTNYERDRKLYENLVISKEEFERVQLQYKNDQEELQAAMDNLS
                     LVKTGMTKNTQKSSNTLVRATVSGTILDIPVKVGNSVIQTNNFNDGTTIASIANLSDM
                     LFVGKIDETEVGKLSVGMPMEITIGAIQDKKIPAKLEYVSPKATEESGAILFEMKANM
                     EMPKDVFIRAGYSANADIVLDRRADVLVIPETCIEFSNDSSFVYIVKNENPQEFDRKH
                     VSVGLSDGINIEIIEGLKLDDKVRGNEIIEKKK"
     gene            5229..6527
                     /locus_tag="PLI69_00625"
     CDS             5229..6527
                     /locus_tag="PLI69_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG41027.1"
                     /translation="MRKILIALFLTISMTGMAQQYTLQECIGIALQNNRTIKQQELNK
                     EQRKIAYTQARNDLLPSLNAQAGQNFIFGRSIGLDNTYQNTNSAQTSFGVGADITLFD
                     GLRMKYNIDAKRADLSAAEADVEKFKDDIEMSVATAFLQVLLQKELLEIANEQLALTD
                     QNISRRNELIQSGKMAEGEIYELEAQRAREEQNKVQAESNLKLALLDLAQIMELDDFS
                     KFDVLTPPVEEIIKNEMLVSPTEVYQEALLIRPEIRASEYRLKSSEKGVLIAKSQLYP
                     TLSFGANLGTGYYKMSERPNDPFSSQFRNNMSNALGFSLRIPIFNRLQTRNNIRTAEL
                     AVENSRIEIDKVKIELRKRIEQAFHNAVGAQSKWRATQKSEKASREAFRFAQEKFDNG
                     RANAYELFQAKSNLTQVLSEQAQAKYEYAFRLKILEMLKE"
     gene            complement(6552..6929)
                     /locus_tag="PLI69_00630"
     CDS             complement(6552..6929)
                     /locus_tag="PLI69_00630"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOG41028.1"
                     /translation="MSSKLKVGMKYVLEHTVREDETAFNYDSGAMHVYSTPALIARIE
                     CAAHTMLMELGLDSVGTIVNIEHKKACKPGTVVIAEALLTEIDGKRLMFDVKVTDREG
                     NILGEGTHGRYIIDPERFMQKIG"
     gene            complement(7012..8274)
                     /locus_tag="PLI69_00635"
     CDS             complement(7012..8274)
                     /locus_tag="PLI69_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOG41029.1"
                     /translation="MYALADCNNFFVSCERVFRPDLNGKPVIVLSNNDGCAISRSNEA
                     KALGIKMGVPFFKIRNLVEEHGVVVFSGNMALYGDMSRRVRQTLERFAPSLEVYSIDE
                     VFLDLHGMQIEDFGSYAREISRICLRDTAIPVSIGISPTKTLAKIASKLCKQYPKLRG
                     GCYMHRPEDIEKVLRGFPVEDVWGIGRRTTPKLYQRGIRTAFDFIKMDEERVRALMGI
                     TGVRTHRELRGIPSIKFEDSVVTKQSICVSSSFSKEITDCRDLCSQVANFAAKAAEKL
                     RGQGSVAFEMIVFAMTNRFRENAPQAYKSILVPFVEPTCEQRTIVGSAVAATRELFNA
                     RMSYKKAGVVITRLEQRNQITGLLFEDSEVAERESRISYLIDSINQQYGYGSIRIGIQ
                     GDGSIHKLSEHRSPHYTTLWSDIPTASV"
     gene            complement(8280..8699)
                     /gene="umuD"
                     /locus_tag="PLI69_00640"
     CDS             complement(8280..8699)
                     /gene="umuD"
                     /locus_tag="PLI69_00640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF007621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOG41030.1"
                     /translation="MIPYEIDENLTTESWIVGDVKAGFPSPAEDIHEKLDLIKLLVRN
                     PASTFFFRIDGVSMVDAAMDEGDIIIVDRSIDPFNGCMAVCYIDGGYTVKRVEVHGKE
                     LHLLPANKHNLSYKPIIVTPDNQFMVWGLVTYVIKKV"
     gene            complement(8702..9256)
                     /locus_tag="PLI69_00645"
     CDS             complement(8702..9256)
                     /locus_tag="PLI69_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG41031.1"
                     /translation="MENPVIETIMARRSIRAYKPEAVEREIMNQIVKCGINAPNAMNA
                     QPWEVRIVDNPEFIEGCTKLFVERMKQDPRRAKMVEDPSFRNMFRNAPTVVFIAINDG
                     RFSSIDCGLMAQNMMLAAKSLGIGSIALGSPVDFFQSEVAAEYYKKLDIPDTHTLALV
                     LGFGYPNESPDAKPRDESKVRWIE"
     gene            complement(9372..11465)
                     /locus_tag="PLI69_00650"
     CDS             complement(9372..11465)
                     /locus_tag="PLI69_00650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOG41032.1"
                     /translation="MKLTRTIAAASLAALMLTSCTMKENPLLQRSTHPYQAPAFDKIE
                     IKHYKPAFIAAIEEAKAEIRLIADNTKEPTFANTIEALNTSGRTLNTVAGIFFNLNQA
                     NTNEEMQQIAEEVAPMMTEYSMSIILNPVLFQKVKRVYEKKDSLGLNQEQAKLLEESY
                     KSFTRNGANLPDDKKEEYARLQEELSVATLTFGRNVLNATNAYLLEITDEADLEGLPD
                     YVREMAAADAQAKGKEGWLFTLDHPSYGPFLKYSTRRELREQLWRAINSKCIGGEFDN
                     TTNIRKIVDLRIRVAQLLGYDTYADYALEERMAKNPATVNSFLADLMQKSLPFAKRDV
                     AEIQAYATTNGFEGELMPWDFSHWSEKFQEERYSLNEELLKPYFELSSVQAAIFDLAG
                     RLYGLKFEENPQLPVYHPDVKVFEVRDENGRFMSLLYIDYFPRESKRGGAWMTSFREQ
                     GFYDGVEERPHVSLVTNFTKPTSTTPSLITFYEFTTILHEFGHALHGMLAEGTYSTLT
                     GTNVARDFVELPSQIMENWAYEPEYLNSFAKHYQTGEVIPQDLIDKIIAARNFLAGYA
                     GVRQLNYGITDMAWHNVTSVPEASVVEYEQEILSNSAIMPVIEGMVFSPSFSHIFSGG
                     YAAGYYSYKWAEVLEADAYSLFKERGIFNREVAAEFRDKILSRGGIEDADVLFRNFRG
                     RDPQPEALLEKLGMK"
     gene            complement(11523..11873)
                     /locus_tag="PLI69_00655"
     CDS             complement(11523..11873)
                     /locus_tag="PLI69_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41033.1"
                     /translation="MDTNRNCKRCGERFVGRSDKIFCCDSCRNDYHNSLRRERNKGMN
                     RVNGILSRNREIIRSLLSKGIESIDARELSMLAFDFDHFTSVDKRILGRTCYHIYEYY
                     YYSANGEVHFSLKE"
     gene            12062..12400
                     /gene="ssb"
                     /locus_tag="PLI69_00660"
     CDS             12062..12400
                     /gene="ssb"
                     /locus_tag="PLI69_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG41034.1"
                     /translation="MKSTFINRVELKGHVGHDPKITEVGESQVARFSLATNETFKDKK
                     GEIKEETTWHNITAWSGRNTADFSLIRKGMLVSVIGRIRNVRYTSSSGDERQFMEILA
                     GKVTIVPPTE"
     gene            12492..13970
                     /gene="cysS"
                     /locus_tag="PLI69_00665"
     CDS             12492..13970
                     /gene="cysS"
                     /locus_tag="PLI69_00665"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOG41035.1"
                     /translation="MARDLFLYDTLKREKRIFKPVNPGYVGMYVCGPTVYGDPHLGHA
                     RPAITFDLLFRLFRHLGYKVRYVSNITDVGHLENDADTGEDKIAKKARLESLEPMEVV
                     QTYTLRYHEAMKELNVLRPSIEPRASGHIIEQIELIKTILDAGYAYVSNDSVYFDVEK
                     YDGEHSYGVLSGRVIDDMMAGSRELDGQEDKRSPNDFALWKKAAPEHIMRWPSPWSVG
                     FPGWHLECSAMSEKYLGEEFDIHGGGMDLIFPHHECELAQNTASRGKGGVNYWVHNNL
                     ITINGQKMGKSKGNFITLEELFAGRHDALEQPYSPMTIRFFILQAHYRSTLDFSNEAL
                     QAAEKGLKRLMQAVKDVASLRHSADAPLLPQVTSIKEDIYTALLDDMNTPVALSSIFD
                     AVKIVNGVKDRGEVLNNGDYNTLTKLFTEIVSDVLGLVDDRQDSSAESVIGGLVEMVL
                     QNRNAAKAAKNWAESDRIRDELKAIGIQIKDTKEGTEWELTN"
     gene            complement(13960..>14273)
                     /locus_tag="PLI69_00670"
     CDS             complement(13960..>14273)
                     /locus_tag="PLI69_00670"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOG41036.1"
                     /translation="NQGDGLLFSPTDLFASSLGSCMLTIIGMVAGTYGFSIDGATVTT
                     EKVMGTNPRRVVEIRLEVNFPKGVEYSKKERTIIVNAAKSCPVANSLDPAINKVVNFN
                     W"
BASE COUNT         4111 a         3032 c         3305 g         3825 t
ORIGIN      
        1 tctctttttt gtaagacgga aaattcttaa aagtatcgca agttttattg ttatcaacat
       61 ggtgctttcc attctaatca tagcactgat aaaaggactt gatgttttgg gctttttaga
      121 gggatatgaa ggaagcgtag ggtataaagg agatataaat atttcctatg gcgaccgagg
      181 cttgtttgat ttttacagag gcgaccatcc tttgatgata tttatgatga tactcagact
      241 gttcatggaa atggttttgc cgatactctt aatgcttggc tcatatcgct taatgaaaac
      301 taacaggtat tgatcatgat aaactttgat gaacataagc ccatttatct tcaaatatcg
      361 gactcgattt gcgagaaaat tctcaacgat gagtacgcag aagatgaacg aataccttcc
      421 ataagggaga tgggggcaca attgggggtg aatcccaata ccgttatgcg tagctacgac
      481 catttgaaat cgctcgatat tatttacgat aagcgcggca tgggtttttt cgcatcgcca
      541 gatgccaaaa ggagcgtgaa aaagatgtat aaaaaggaat tttttgaaat ggaattgccg
      601 gccattatga aaaaaatgaa gcttttggat atagataccg atgaattggt actaaaaatg
      661 catggcacag atgacaaaca ttctgagttt taaattatga ttgatattaa acaacttcac
      721 aagtcctact ataccgaagc catatcactt cacgtgctta aaggtcttga cctcaaaatt
      781 gaggcgggcg aatatgtctc cattatggga gcatccggtt cgggaaaatc cacgctgctg
      841 aatataatag ggattctcga tacctacgac gaaggtgaat ataggttaaa tggtaccctt
      901 atcagagacc tgagtgaaaa acaggcggca aagtatcgca acgaaatgat cgggtttgta
      961 tttcaatcct tcaatcttat caatttcaag aatgcccttg aaaatgttgc gcttccgctc
     1021 tattataaaa atataggccg caaacaacgc aacataatcg cgatggagca tctggataaa
     1081 atgggactga aagattgggc acatcattta cccaacgaac tttcgggtgg acaaaagcaa
     1141 cgtgtagcca ttgcccgcgc aatgatttca aatccaaaaa ttattctcgc agatgagccg
     1201 acaggagcat tggacagtca aaccaccatc gaggtaatag gagtcctcac agacttgaat
     1261 aaacagggta ttaccactat catcgttacc cacgagcaat ccgtagccga ttccactaac
     1321 cgtattatcc gtctaaaaga cggcatgatc gaaagcgaca cccggcaccc gcaccccgta
     1381 ccccgtaact cgtaacccga aaatggatta tttacaagaa atattcgcta ctctccgcaa
     1441 aaacaagttg cgcaccatac ttaccggtct atcggtttcg tggggaattt ttatccttat
     1501 tatcttgctt ggtgcgggca acgggttgaa aaacggggtg acatcgaatt tcgaacaacg
     1561 tgccgtaaat cgtgtcaacc tttatgcagg ttatacctca atgccgtaca aagggttgaa
     1621 atcaggacga agcattgagt ttaccgaaaa tgaactatcg gctgtagagc aagaattaga
     1681 ggagagccgt atggttactg cccgttcaag tacaacacaa aacatctctt atggagttga
     1741 atatggctcc tatgatgtgc gtggtgttat gccgagttat gccgaaatgg aaaaattgat
     1801 ttttgagccc ggtaacggaa gatttttcaa tcaacttgat tataagcaga agagtaaagt
     1861 gattgtcctc gacacaaaaa tagcggaggt cctctttaaa gggaaatcac cattggggga
     1921 atatgtaaag gttggtcaac tgatgtttaa agttattggt gttaattcta aaaaagcgca
     1981 gtggggaggc cccgttgcct atataccctt ttccatatct caagcgattt ttaatccaga
     2041 taaaaaattc aatcaactca cctttactgt agaagggctc gaatccaaag atgaaaatga
     2101 actctttaat agttctgtaa gaaatcttat gggtcgcaga ctcaaatttg atccttcaga
     2161 cgaacaagca ctttgggtaa acaacacaca agccgactat cttgaaacga tgaagatttt
     2221 tggcggaatc aatgtatttg taaccattat aggaattctc acgttacttg ccggaattgt
     2281 gggtgtcagc aatatcatgc tggtttctgt aaaagagcgt acgcgagaaa taggaatccg
     2341 taaagccatt ggcgcaccgc ctgcctccat attgacctcc atcattttgg aatctatcat
     2401 tataaccgcc atcttcggct acatcggtat gtttttagga ataggtctca cagagttaat
     2461 caactttttt atggagatgt cagcaagtgc agctcaaagc gtagatgcag gtccgcaaat
     2521 gtcggtgttt aaaaatccta ccgtaaatgt cgggtacgca atatttgcaa ctattatact
     2581 tattatatcg ggagttattg caggctatct gcccgcaaga aaggctgtta aaataaagcc
     2641 gatagaggca atgagacagg agtaggtttt agtattgagt tctgagtatc tagtattgag
     2701 ttttgaataa aatagaatga aaaaagagat aaatagtcag ctacaatttg agaaaacctc
     2761 cctttggaga aggttcatgt tgcttttcga catcgatcgc tggcaggaga tctggattac
     2821 aattacaagt aataagtcgc gcagttttct cacggcattc ggagttttct gggggatatt
     2881 tatgcttgta ctgatggtgg gttcgggtaa tgctttagag acaggaatta tgtcgcaaat
     2941 ggaaggtttt gctaccaatt cggtcttttt tgcggcagag cgtacgactg agccttacaa
     3001 gggtttcaga aaagggcgat cttgggatat gacaaatagc gatttgccaa ttattcgtga
     3061 aagagttgat gaaatgcaat atatgtctcc cgttcttttt gccggaggcg gtgataaaaa
     3121 tgtggtgaga ggtgataaaa gcggttccta tctggtaaaa gggtgctatc ccgaatatga
     3181 tttgatagaa aaaagcaaaa tgctatatgg gcgatatgta aatgatatag atattgctga
     3241 aaaacgcaaa gtgtgtgtta taggtgagcg aatatatgag gtgcttttcc atggtgaaga
     3301 tccgacagga aaacagattc gagtaaatgg aatatatttt caggtcatag gcgtagcccg
     3361 cagtacatcg caagtaagca ttggcggaca aactgcagaa acagttgttt tgccattcac
     3421 aacaatgcaa caagcattta atcaaggaaa tataattcac tttttggcag ctaccgccaa
     3481 accgggtatg gatgtgaaaa taatagagga taaaataacg aatattttaa aacaacagca
     3541 ccagattgcg cctgaagata aatcggcagt ttttagcatg aacatcgagg agcaattcaa
     3601 aatgttcaac aacctcggta tcggcattgc cgcactcatc tggattgtcg gtctcgggac
     3661 gctcttagcc ggagctatcg gagttagcaa tattatgctc gttacagtgc gcgaacgcac
     3721 caaagagatt ggtatccgcc gtgcccttgg agctacgccg aataatatta taggacaaat
     3781 aatgagcgag accgtagttc tgactctttt ggcagggatc ggaggaattg tgctgggtgt
     3841 cggtttgctt tctgccatag gtgtagcgtt gagccagggt gatcaatttt ttaaagaccc
     3901 tcaaatcagc ttctcaatgg cgatagtctc ccttattatc ctgatcgtta taggattatt
     3961 ggcaggactt gtccccgccc aacgcgccat gatgataaaa ccggtggaag cgatagcgga
     4021 ggaatagaag tgttacaggg tgcaggttgc gagttgcgag caagagtcct gaggaaattg
     4081 atttagaaga aatagaaaca aacaataaaa acaataatat acaatgagaa aagtattaaa
     4141 aatcgtaggt ttagtccttt taggagtatt ggtaatcggg acatttgtgt ttctttataa
     4201 gaagtcgcgt cctgtccgga aaacatatca aattgaaacc gtcatacaag gaacgataga
     4261 gaaaaaaacg gtggcaacag gcaaggtaga gccacgtaac gaaatcctta tcaagcctca
     4321 aatgtccggt atcgtatccg aagtatacaa agaagcgggg gatagagttc tggcaggcga
     4381 tattattgca aagattcaag tagtaccgga tatggtcaat ttgagcggag cagaatcgcg
     4441 tgttacaagg gcacaacttg ctgcggagca gagtaaaacc aattatgaac gcgaccgtaa
     4501 actgtacgaa aacttggtaa tatcgaagga agagttcgaa agagtgcaac ttcaatacaa
     4561 aaacgaccag gaagaacttc aagctgctat ggataacctg agtctggtta aaacgggaat
     4621 gacaaaaaat acccaaaaat ccagcaatac actggtacgt gccactgtca gcggaactat
     4681 cctggatatc cccgtaaaag ttggaaattc ggtaatacaa accaacaatt tcaacgatgg
     4741 gactacgatt gcctccattg cgaacctgag cgatatgctt tttgtgggaa aaattgatga
     4801 aacggaagtg ggcaagctct cagtgggaat gcctatggag ataacgatag gcgctattca
     4861 ggacaaaaaa ataccggcaa agctggaata tgtatcgccc aaagccaccg aagagagcgg
     4921 agccatccta tttgagatga aagccaatat ggagatgcct aaagatgtct tcatccgtgc
     4981 cggttatagt gcaaatgcgg atattgtttt ggataggaga gcagatgttc tggttatccc
     5041 cgaaacatgt atcgaattca gcaacgattc ttcatttgtt tacatagtaa aaaacgaaaa
     5101 cccgcaggag tttgaccgta agcatgtctc cgtggggctt tctgacggta taaacattga
     5161 gataatcgag ggattgaaat tggacgacaa agttcgtgga aatgagataa ttgagaaaaa
     5221 gaagtaacat gagaaaaata ttaatagcat tatttttaac catttccatg acaggaatgg
     5281 cgcagcaata cacccttcaa gagtgtatcg ggattgcgtt gcaaaataat cgcaccataa
     5341 aacaacagga gctgaacaaa gagcaacgga aaattgctta tacgcaagca cgaaacgatt
     5401 tgttaccaag tctgaatgcg caagcgggac aaaatttcat attcggacgc tccatcggac
     5461 tggataacac ctatcaaaac accaactcgg cgcaaacttc attcggagtt ggagcagata
     5521 ttacgctttt tgacggcttg cgcatgaagt acaatataga tgctaaacgc gctgacctga
     5581 gtgcagcgga agcagacgtg gagaagttta aggatgacat agaaatgagc gtagccaccg
     5641 ctttcctgca agtgttgctt caaaaagaat tgttggaaat tgccaatgaa caactcgctt
     5701 taaccgacca aaacatctct cgccgcaatg aactcattca aagcggtaaa atggcggaag
     5761 gcgaaatcta cgagctggaa gcacagcgtg cacgtgaaga gcaaaataag gtacaggcag
     5821 aaagcaattt gaaattggca ttgctcgatc tggcacaaat aatggagctt gatgatttct
     5881 caaaatttga tgttttaact ccaccggttg aagaaataat caagaatgaa atgttggtgt
     5941 caccaaccga ggtgtaccaa gaggcattgc ttatccgtcc ggaaattcgc gcctcggaat
     6001 atcgcttgaa aagtagtgag aaaggggtac tgattgcaaa atcgcagctc taccctacct
     6061 tgtcatttgg agccaatttg ggcaccggat attataaaat gagtgagcga ccgaatgatc
     6121 ctttcagctc acaatttcgc aataatatga gcaacgccct cggttttagt ttgcgtatcc
     6181 caattttcaa caggttgcag acacgcaata atatccgtac tgccgagctt gctgtggaaa
     6241 acagccgtat tgaaatagat aaggttaaga ttgagcttag aaaacgcatc gagcaggcat
     6301 ttcacaatgc agtcggtgca caaagcaagt ggcgggcaac acaaaagtca gaaaaagcga
     6361 gtagagaagc ctttagattt gcacaggaga agtttgacaa tggccgtgcg aatgcctacg
     6421 aactttttca agctaaaagc aaccttacac aagttttgag cgaacaggca caagccaaat
     6481 atgaatatgc tttccgattg aaaatcctgg aaatgttgaa ggagtaaggt aatcttgtct
     6541 cgctctgatt atcaaccgat tttctgcata aaacgttccg gatctataat gtaccgaccg
     6601 tgagtgcctt caccaagaat gttgccctcc cggtcggtca cttttacatc aaacataagg
     6661 cgttttccgt caatttccgt caggagtgcc tcagcaataa ccacagtacc gggcttacag
     6721 gcttttttat gttcgatatt tacaatggta cccacgctat ccagacccaa ttccatcaac
     6781 attgtatgtg cagcacattc tattcttgcg attagtgccg gagtagaata gacgtgcatt
     6841 gctcccgaat cataattgaa agcagtttca tcttccctta cggtatgctc caggacatac
     6901 ttcattccaa ctttaagttt tgagctcatt atctttatcc tttattgttt taaattgcag
     6961 ttgcgaaaat acgacaatta tctgaataac aatcccaaaa gtcatggagg cttatacact
     7021 cgctgttgga atatcactcc atagggtagt gtagtgtgga gaacggtgtt cgcttaactt
     7081 atgtatgctg ccgtccccct gaataccgat acggatggat ccgtagccgt attgttgatt
     7141 tatagagtct ataagatagg aaatacggga ttcgcgttcg gctacttcac tatcttcgaa
     7201 gagcaagcct gtgatttggt tgcgctgctc aagacgagtg ataaccacac ctgctttttt
     7261 gtaggacatt cgggcattaa atagttctct tgtggcggca acggctgaac ccactatggt
     7321 gcgttgctcg caggtaggtt cgacaaaggg tacaagtatg cttttatatg cttgaggagc
     7381 attctcgcgg aagcgatttg tcattgcaaa gactatcatt tcaaatgcca cggacccttg
     7441 tccacgaagc ttttccgccg ctttggcggc gaaattggcc acctgactac ataaatccct
     7501 gcaatcggtg atttccttgg aaaagctgct tgagacacat atcgactgtt tggtgacgac
     7561 gctgtcctca aatttgatac tggggattcc acgtagttcc ctgtgcgtac gcactccggt
     7621 aatacccatc agcgcacgga ctctctcttc atccatttta ataaagtcaa aagcagttcg
     7681 gatgcccctc tgatagagtt tgggagtggt tcttcgcccg attccccata catcctctac
     7741 cggaaatcca cgcagcacct tctcaatatc ctctgggcgg tgcatataac aacctcctcg
     7801 caactttgga tattgtttgc agagtttgga ggcgattttc gccagagttt tggtagggga
     7861 gatgccgatt gaaacaggaa tggccgtgtc acgaaggcag atacgggata tctcccttgc
     7921 ataacttcca aaatcttcaa tctgcattcc gtgcaggtcg agaaagacct catctatgga
     7981 atagacctcc aatgaagggg caaacctctc cagagtttgc ctcactctac gcgacatatc
     8041 gccgtagagc gccatatttc ccgagaaaac tacaactcca tgctcctcca ccaaattacg
     8101 tattttgaag aatgggactc ccatctttat accgagagcc ttggcttcat tgctgcggga
     8161 aatggcacaa ccatcattat tggagagaac aatgacaggt ttcccgttta gatcgggccg
     8221 gaacacccgt tcacacgaga caaaaaagtt gttgcagtcg gccagcgcat acatggcggt
     8281 cagacctttt ttatgacgta ggtcaccaac ccccagacca taaattggtt gtctggcgta
     8341 acgataattg gtttatagga tagattgtgc ttatttgccg gtagcaggtg cagctctttt
     8401 ccgtggactt ccaccctttt taccgtatat ccgccatcaa tgtagcagac tgccatacag
     8461 ccgttgaagg ggtcaatgct cctatcaact atgatgatgt ctccctcatc cattgcagca
     8521 tccaccatcg agactccgtc tatcctgaaa aagaaagtgg aggcggggtt gcgtaccagg
     8581 agtttgatca gatcgagctt ttcgtgaata tcctccgcag gcgagggaaa accggccttt
     8641 acatcaccca ctatccaact ttccgtagtc agattctcat ctatttcata aggtatcata
     8701 cttactctat ccagcggact ttgctctcat ccctgggctt ggcatcggga ctttcattag
     8761 ggtaaccgaa ccccaagacg agcgcaaggg tatgggtgtc ggggatgtcc aactttttat
     8821 aatattctgc agccacttcg ctttggaaaa agtcgaccgg actaccgaga gctattgaac
     8881 cgatcccgag cgatttggct gcaagcatca tgttctgagc catgagaccg cagtcaattg
     8941 aggaaaacct gccgtcattg attgctataa agaccaccgt aggtgcattt ctgaacatat
     9001 ttctgaaaga agggtcttcc accatttttg ccctgcgcgg atcctgtttc atgcgttcta
     9061 caaaaagttt ggtacaaccc tcgataaatt ccggattgtc cacgatgcgg acctcccatg
     9121 gttgtgcatt catggcatta ggggcattaa tgccgcattt aactatttgg ttcataattt
     9181 cgcgttctac ggcctccggc ttatatgccc tgatgcttcg acgcgccata attgtttcga
     9241 ttacaggatt ttccattggg tttgcagttt cttgattctg attgccgcaa ctgacactca
     9301 aaagtaacgt tgcagccata atatacaaaa atattttctt cataactagt atagattaaa
     9361 tgttgacctt actatttcat accgagcttc tcaagaagag cttccggctg gggatccctt
     9421 ccgcggaagt ttcggaacaa tacgtcagca tcctcaatgc ctcctctgga gaggatttta
     9481 tcgcggaatt cagcagccac ctctctatta aaaatacctc tctccttaaa gagggaataa
     9541 gcatctgcct ccagcacttc agcccattta taggagtagt aaccggcggc atatccacca
     9601 gagaaaatat ggctgaatga gggtgaaaag accatgcctt ctattacggg cattatggcc
     9661 gaattgctga gaatctcctg ctcgtactcc acaacggaag cctccggtac cgatgtaaca
     9721 ttgtgccagg ccatatcagt gattccgtaa ttgagctgtc ttacgccggc atatccggca
     9781 aggaagttcc tggcagcgat aatcttgtca atcagatcct ggggaataac ctcacctgtc
     9841 tggtaatgct tcgcaaaaga gttcagatac tcgggctcgt atgcccagtt ctccatgatc
     9901 tgtgagggaa gctctacaaa gtcgcgggcc acgttggtgc ctgtcaaagt actgtaagta
     9961 ccctctgcca gcatgccgtg gagtgcgtgt ccgaattcat ggagtatggt agtaaattcg
    10021 tagaaagtta taagcgaagg agtagttgag gtgggtttgg taaaatttgt taccaatgaa
    10081 acgtggggtc tctcctcgac tccgtcgtag aaaccttgct ccctgaaaga ggtcatccat
    10141 gcaccacctc tcttgctctc acgagggaag tagtcgatat agagcaggga catgaatctt
    10201 ccattctcat ctcttacctc aaatactttg acgtcgggat gatatacggg caactgcgga
    10261 ttctcttcga atttcagtcc atagaggcgt cctgccagat caaaaatggc cgcctgaaca
    10321 ctggatagtt cgaaataggg cttgagcagc tcttcgttaa gcgagtatct ctcttcctgg
    10381 aatttttccg accagtgtga gaagtcccag ggcatcaatt ctccctcgaa gccgtttgtt
    10441 gtggcgtagg cctggatttc ggcgacatcg cgtttggcaa aggggagaga tttctgcatc
    10501 aggtctgcaa ggaaactgtt gactgtagcc ggattctttg ccatcctctc ctccagtgca
    10561 taatctgcat aggtatcata gcccagcagc tgtgccacgc gaatgcgaag atccactatc
    10621 tttcggatat tggtagtatt gtcaaactca ccgcctatgc atttgctgtt tatcgctctc
    10681 cagagctgtt cgcgcagttc gcgacgagta ctgtacttaa ggaaggggcc atagctcggg
    10741 tgatctaggg taaagagcca tccctcctta ccttttgcct gcgcatctgc cgcagccatt
    10801 tccctgacat agtcgggaag tccttccagg tcggcttcat cagtaatttc aagtaaataa
    10861 gcgtttgtgg cgttgagtac attgcgtccg aatgtaaggg tggcaaccga gagttcctcc
    10921 tgcaatcgcg catattcctc tttcttgtca tccggcagat tggctccgtt gcgggtgaaa
    10981 gatttgtaac tctcttccag cagtttagcc tgctcctgat tgagtccgag actatctttc
    11041 ttttcataga ctctcttcac cttttggaat agcacggggt tgagaatgat cgacatgcta
    11101 tattcggtca tcatgggggc tacctcttca gcaatttgct gcatctcttc gttagtattg
    11161 gcttggttga ggttgaagaa gatgcctgca acggtattta gtgtgcgtcc ggaggtgttg
    11221 agtgcctcta tggtattggc gaaagtgggt tctttcgtgt tgtcggcaat aagccggatt
    11281 tcagctttgg cctcttcgat agccgcaata aaggctggct tgtagtgctt tatttctatt
    11341 ttgtcgaagg ccggggcctg ataggggtgt gtagatctct gtagtaaagg attttctttc
    11401 attgtgcagg aagtaagcat cagtgcggcg agtgatgccg cagcgattgt tctggtgagt
    11461 ttcatattgt gcttaataat ttgtttcgaa acttcaaagt taacgatttt aagtcaaaaa
    11521 actcattctt tgagagagaa atgtacctca ccattagctg aataatagta gtattcgtag
    11581 atgtgatagc aggtacgtcc gagaattcgt ttgtccacag aggtaaaatg gtcgaaatca
    11641 aaggcaagca tactcaactc tctcgcatcg atgctctcaa tacctttgga gagcagagaa
    11701 cggataattt cgcggttgcg cgaaagaatt ccattcaccc tgttcatacc tttgtttcgc
    11761 tccctccgaa gagaattgtg ataatcgttc ctgcaactgt cacagcagaa gatcttgtca
    11821 cttctaccca cgaatctctc tccacatctt ttacagtttc tatttgtatc catatagatc
    11881 ctctattaaa cttgacgtaa aatttggttt cgacaacaaa aataattgaa aatatttaca
    11941 aacaggtgtt gaaaaagcaa ttttaacgag tcgaaacata tagaaacggg tataattatt
    12001 tagagtattg atacttgata ttaacctctt ttactttgcc tttgaacaaa aaccaaatat
    12061 tatgaaaagt acatttatca atcgggtaga gttgaaggga catgtaggcc acgacccgaa
    12121 aatcacagag gtgggcgagt cgcaggtggc aagattctcc cttgcgacca atgagacatt
    12181 taaggacaag aaaggagaga tcaaggagga gaccacctgg cacaacatca cagcctggag
    12241 cggacgtaat actgcagact tctcgctcat ccgcaaaggt atgctggttt cagtcatcgg
    12301 acgcataaga aatgtcaggt atacgtccag cagtggtgac gaacgccaat ttatggaaat
    12361 tcttgcaggc aaagttacca tagtaccccc aacagagtag tagtgtagca ttttagttca
    12421 actttcggca atagcgagcc aaatattgcc gaaagttgct aattttgtag tttagtgcaa
    12481 aaaagatgat tatggctcgt gacttatttc tttatgatac cctaaaaagg gaaaaacgca
    12541 ttttcaaacc tgtaaatccc ggctatgtcg gaatgtatgt atgtggccct acagtatatg
    12601 gtgacccaca tctgggccat gcccgccctg caataacatt tgatctgcta ttcaggttat
    12661 tcagacattt ggggtacaaa gtgagatatg tgagcaacat caccgatgta ggacatcttg
    12721 aaaatgatgc cgatacaggc gaggacaaga ttgccaagaa agcccgcctt gagtctttgg
    12781 agcctatgga agtggtacag acctatacct taagatatca tgaggcaatg aaggaactta
    12841 atgtgctgcg cccttcaatt gaaccgaggg cctccggtca cataatagag caaatagagc
    12901 ttatcaaaac cattctcgat gccggatatg cctacgtcag caatgactcc gtctattttg
    12961 acgttgaaaa atatgacggg gaacattcat acggagtgct ttccggtcgt gttattgacg
    13021 atatgatggc agggtcacgg gagttggatg gtcaggagga caaacgttcc ccaaatgact
    13081 tcgccctctg gaaaaaggcc gctcctgagc acataatgcg ttggccctct ccctggagtg
    13141 tcggatttcc gggctggcat ctggagtgtt ccgccatgag tgagaagtat ctcggagagg
    13201 agtttgacat tcacggtggc ggaatggacc tgatatttcc ccaccacgag tgtgaattgg
    13261 cccagaacac cgcttcccgt ggtaaaggtg gcgttaacta ttgggtacac aacaacctca
    13321 tcacaataaa cggccagaaa atgggcaaat ccaaaggtaa cttcataacg ctagaagagt
    13381 tgtttgctgg aagacacgat gccctcgagc agccctattc gcctatgact ataaggtttt
    13441 tcatccttca ggcgcattac agaagcactc tggacttcag caatgaagcc ttacaggctg
    13501 ctgaaaaggg acttaagcgc cttatgcagg cggttaagga tgtcgcttca ctcagacatt
    13561 cggctgatgc tcctcttttg ccgcaggtga cttccataaa ggaagatata tatacagctc
    13621 tcctggatga tatgaataca cctgtggccc tctcttctat cttcgatgca gtaaagatcg
    13681 tcaacggagt caaagacaga ggggaggtac tcaataatgg cgattacaac accctaacca
    13741 aactatttac tgagattgtc tcggatgtac tcggcttagt cgatgatcgt caggattcat
    13801 ctgccgagtc tgtaataggt gggctggtgg agatggtact acaaaatcgc aatgcagcta
    13861 aagctgcaaa gaattgggca gaaagcgacc ggatccgcga cgaacttaaa gccatcggga
    13921 ttcagatcaa ggataccaaa gagggtactg agtgggagct taccaattaa agttcaccac
    13981 tttattgata gccggatcaa gactgtttgc caccggacaa gatttggcgg catttacgat
    14041 gatagtcctc tcttttttgg aatattccac acccttgggg aaattcactt caagacgtat
    14101 ctccacgaca cggcggggat ttgtccccat taccttttcg gtggtgacgg tggctccgtc
    14161 aatcgaaaag ccgtaggtgc ctgctaccat tcctatgatt gtcagcatgc aacttcccag
    14221 cgaggaggca aaaaggtcag taggtgaaaa gaggaggcca tctccctgat tat
//
LOCUS       DAOSIE010000008        14152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_4395_length_14152_cov_4.185288, whole genome shotgun sequence.
ACCESSION   DAOSIE010000008 DAOSIE010000000
VERSION     DAOSIE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14152
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4395_length_14152_cov_4.185288"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..694)
                     /locus_tag="PLI69_00675"
     CDS             complement(251..694)
                     /locus_tag="PLI69_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41037.1"
                     /translation="MKSITKIFFISGVAFWSIGLMYSFSKLIALNGLGNIFEMNEAQN
                     ANTRIIRDSTHITISYRYQVDEKTYQDSYKMLIEYFEKCDIDTIAIKYNKRFPMVSYI
                     DSVPLKMRQQKVGICISAFFILFFIFLWKLSDKDKWIKTYEDVGN"
     gene            complement(711..1256)
                     /locus_tag="PLI69_00680"
     CDS             complement(711..1256)
                     /locus_tag="PLI69_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41038.1"
                     /translation="MKKIIKTITIGIAVLIGLVLIARPALGLYGECWAYFDKKAFLAK
                     YEFEDFSQFKNVSLFIRGGDSERNPVIVVDAPHLVRDTSKVGYYVVTLDKRTYQVKEA
                     KWMTEYDVEADTLKLQQLAQTFMMYRIPRLDVDTAGNVFVYVKDVATLALVRFANENE
                     LQKRSKETKWINIKSNWYKPR"
     gene            1467..3797
                     /locus_tag="PLI69_00685"
     CDS             1467..3797
                     /locus_tag="PLI69_00685"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOG41039.1"
                     /translation="MNKFLRLLVILLLMSATHLSAQNSPLYYVNPMVGTKSMGHTFPG
                     ACAPFGLVQLSPDTEMIPHNIDGVYQSDAYKYCAGYQYDDKTIVGFSHTHFNGTGHSD
                     LGDILIMPMTGEVKLDMGTEDNPETGYRSRFSHDYEKAEAGYYSVLLQDYGILAEMTA
                     TERVGVHRYTFPRDTKTGHIILDLDYGIYNYDGKVLMANLRVEDEYTLTGYRITRGWS
                     RMNYTHFAVKFSKPIQNYGCSNKEKVLYTGFWRKFDMTDNFPEMFGKHLTAYFDFDFS
                     DGEPLDIQVALSPVDCRGAMKNLEAETGGKSFDDIRNETQRKWEKELNCIQFEADEET
                     KYVFYTALYHTMINPSVYQDVDGRYRGIDHNIHQAEKGATNYTVLSVWDTFRAQHPLM
                     NLIKPSQSTQFVNSMLNHYKQSVHKALPVWSHMGNENWCMIGYHSVSVVSDAIAKGLD
                     IDTELAFEACVNSSNVDYFDGTGDYKKYGYVPLETSSSAASITLEYSYDDWTIHKLAN
                     RLNKSEISQEYARRALNYRNIFDPSLHFARPKLKDGTFKEDFDLLDTHGQGFIEGNSW
                     NYSFFVPHDVNGLIKEMGGEKQFIRRLDSLFTMHLPAKYFEKTEDINEEGLMGNYVHG
                     NEPSHHVAYLYKWTKEPWKSEYRLHEIMRQMYKNKIDGLSGNDDCGQMSAWYIFSALG
                     FYPVCPGSDQYVIGAPKTKEATIYLENGKTFTVKTENLSDKNVYVKSIKLNGKKYEKH
                     FITHEDILKGGEMLFEMSNKPNRKSASYEKPYSFTK"
     gene            complement(3870..5027)
                     /gene="ilvA"
                     /locus_tag="PLI69_00690"
     CDS             complement(3870..5027)
                     /gene="ilvA"
                     /locus_tag="PLI69_00690"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HOG41040.1"
                     /translation="MKRTDLERSKSFSNMVNAQVYLKLENLQSTGSFKIRGAYNHISR
                     LSPEDKERGVLCASAGNHAQGVAYAATKLGVKSTIFMPEFAPPIKVIATRSYGANVIL
                     KGDSFNDAYEAAMEYLDESGAVFVHPYNHPDIISGAGTIGLEIFEQLDDIDMVFVPIG
                     GGGLISGIAIALKSMNPNIKIIGVEAEGAHSMRVSLDNGVLTPFVSGNTIADGIAVKA
                     PGNLNFEIVKKYVDDVVVVNDNEIAQACYRLLQRAKNLSEPSGAASLAAILNKKVDVV
                     NKNVVAVISGGNINTSILEQIIEKGVIGEGLRARIAVLIPDQAGMLSKIISILEKLRA
                     SIHDIEHERSITNVPVGHVQVTITFNLQDTSQLFTLGEELKAKGLPYKILK"
     gene            complement(5426..6739)
                     /locus_tag="PLI69_00695"
     CDS             complement(5426..6739)
                     /locus_tag="PLI69_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOG41041.1"
                     /translation="MDKKKNQLSRREALKLFGTGIIGVAMATTGLSSISARNVESREP
                     NVAKRRHKGSSNMVSLLGFGMMRFPTRSDKSIDEELSLSMIDYAYKHGVNYFDTAFNY
                     HGGTSEIFTGKALKRYPRESFYLASKMPGWMVTSLEKAKEIFETQLQRCQVDYFDYYL
                     LHSVSREEDYVRVYEEIGTLEYLIEEKKAGRIRNLGFSFHGSNELFDKMLEKYDWDFV
                     QIQINYHDWDQMDAKYLYRRLEEKDIPCIVMEPIRGGMLAKLNPDAEAVLKRVHPEKS
                     IASWALRYVASLPGVLTVLSGMSAMEHVVDNVNTLSTEFKSLNDKERVALDKALNIFL
                     KTRPIRCTACKYCMPCKFGVDIPANFMHYNKCVNESNIPERDSQTPEEFERRKKIFLE
                     GYYAIPEEERADKCRLCGKCERSCPQRLKIADEMERITKLVKSLQ"
     gene            7008..8537
                     /locus_tag="PLI69_00700"
     CDS             7008..8537
                     /locus_tag="PLI69_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOG41042.1"
                     /translation="MLCKTYCATCIGITAVTVTVEVDLSNGIGIHLVGLPDNAVKESL
                     LRVTTALSTYGYRIPGRKLVFNLAPADIRKEGSSYDLAIAIGLLAVSEQAYLPGCSKY
                     IMLGELSLDGRIRNVTGALPIAEHAREMGFKGCIFPKESALEAADVEGIDIYGVSMLS
                     EVLEILRGDNCPHLLVVRDPETSRRPHYAEDFKNVKGQRMAKRGLEIAAAGGHNLLLV
                     GTPGSGKTFMASCLPSILPQMSRDESIETSKIYSISGNSTGRRGLMLERPFRSPHHSA
                     SLVSLIGGGQNASPGEVSLSHNGILYLDEMAQFSKIILDALRQPLEDGKVTISRARYK
                     VEYPASFMLIASMNPCPCGYYGDDSHKCTCTQSAVMRYMSRISGPMMDRLDMHIFVKA
                     VSGDDLIAEGEEETSEAIAARVMAARQIQYERFSGEGIFTNSQMNVRQLNNFCKVSTE
                     QKRFLRDVINRLNLSARSYSRILKLSRTIADIDGDNFISLQHISEAIQFRNLDRGNFY
                     D"
     gene            8530..8943
                     /gene="tsaE"
                     /locus_tag="PLI69_00705"
     CDS             8530..8943
                     /gene="tsaE"
                     /locus_tag="PLI69_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOG41043.1"
                     /translation="MIEIIINGTGDLQRAARVFLEKAGESRIIAFYGSMGAGKTTFIT
                     ALCRQLGVLDVVNSPTFTIVNEYADAEGNRFYHFDFYRINRLSEAIDIGLFEYFDSGD
                     YCFIEWPEMIEELLPEETLKVNILVDDADTRRLLF"
     gene            9054..10178
                     /locus_tag="PLI69_00710"
     CDS             9054..10178
                     /locus_tag="PLI69_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOG41044.1"
                     /translation="MDLPQEQILVGAESLDEYLPLLEGKKVGLLSNQTGILSNGTHLL
                     DTLLSHGVNMVLILSPEHGFRGNADAGELVSSSVDEKTGIPIRSLYDGKGGYPTQETM
                     DEIEVMVFDLQDVGCRFYTYVTTMTKTMEVCAKNGKKMIIMDRPNPLGFYVDGPILDM
                     QYKSTVGFLPIPVVHGMTLGEIGMMINGEKWLPDGIQCDLTVIKCKNYTHQSHYSLPV
                     KPSPNLPDMRSIYLYPSMCYFEATPVSLGRGTDKAFQMYGHPNMKGYKYSFTPRSVDG
                     AKNPPQLDRECFGVDLRTEPSIEQINAVGLNLEYVIDAYRNLNLDSHFFRSFFELLIG
                     RDYVRKMIMSGASAEEIKAMWAEDVASFKEQRRPYLLYEE"
     gene            complement(10380..11588)
                     /locus_tag="PLI69_00715"
     CDS             complement(10380..11588)
                     /locus_tag="PLI69_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41045.1"
                     /translation="MNEKDLYELLREKLDASVEMPDASTWDGIRSKMARRHRMMVVRR
                     VSFISVAAAAVLAAGLFIFRGDEISTLPSLPSAIAETTIPDTGIPADREIRPMAEQVA
                     DLTPSGAVAKHTKPVARTATPVADKLSETTVDKVAEEKVVADVDVVVSDNEVVAEQVV
                     AQDDKPASDPVVREWDEKDLDRILGQEKPVRRISKNTSLLAISSNISQVASKDGIFAD
                     LGPRYAPSELGTPIESQSIIPFDDDPQFDIPLSFSLQFKQAIVPRLYIGSGLVYTYLH
                     SQYSAMINLERHPNVSSQLHYLGIPLNLSYNFVDRPRFDAFVSVGGMVDKCIMRRYVY
                     GSTDRREKVDGVQWSVNLGLGAEYWFVKHVGIAFKPSLAYYFDNNQPLSIRTQQPIQF
                     NLELGLRFRM"
     gene            complement(11589..12158)
                     /locus_tag="PLI69_00720"
     CDS             complement(11589..12158)
                     /locus_tag="PLI69_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG41046.1"
                     /translation="MKQSMKKQSALTDTDLNDLIQRCLKRDVVAQKRLYDTYAPKMLA
                     LSIRYMGDREAGMDVLQDGFVTVFSKLRTYKGEGSFEGWMRKIFVNEALMALRKNDAL
                     RYADKIDNIPAAFMMESREDAVSRMGAKELTALIESMPPGYRAVFNLTIDGFQHDEIA
                     RMLGISSASSRSQLSRAREWLQKRIEELY"
     gene            12281..13954
                     /locus_tag="PLI69_00725"
     CDS             12281..13954
                     /locus_tag="PLI69_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG41047.1"
                     /translation="MKKILIIFALTIVNFLCAAQSHTYLDALQAYQKGEIAQAHTLFV
                     NEIKANPDNDAAHFYLAMIYSANTNSHIKAEEEFKKALAIDPNNYWYKYSLALFYAET
                     DRMELTTLLLEELIEAFPKKSELYFTAINAFLSQNDITKALTTLDKIEGKMGKTEMIG
                     MTRMDLMSKQGKYTDKELYDYLESYYKECQSPRMATMLGDRYASLYNDSLSLFYYNQA
                     IDIDQGYSPAYYGRAHVFQALRQYERYFEDMGIFMKDPAISPRLKSEYLNKLTESAQF
                     VMAFPEETEQMVLEAYTTHPKDEAITTMAGVFFYRMDKTHYAIELMKQNSENHPDNPE
                     AALQYLMMLYYQKLWDELIPACDRTLERFGYNSDIVQLRGIAYSLKEEFDLAIKDFKL
                     IASSAPKDSATIVFTNSMLGDLHHQKGDSKNAYKHYKKVLKTNPNYAPTLNNYAYYLA
                     LEGKSLKKAKAMSEITIKQEPDNPTYLDTYAWILHLLGQNIEAKAIFKHAMLYGGKES
                     AVILDHYGDVLFELKEYDLAFVYWNQAKALDDTLGIEEKIKVKKAEIGR"
     gene            13957..>14152
                     /locus_tag="PLI69_00730"
     CDS             13957..>14152
                     /locus_tag="PLI69_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41048.1"
                     /translation="MKRLLLILIILLPSLSLSAQDISRQSEQKKRLEEEIEFINKQLK
                     EILSKEKASTLELTLITRKIS"
BASE COUNT         4099 a         3131 c         2912 g         4010 t
ORIGIN      
        1 cgtgaacaga aaatttgatt tttttgtcca aatctttaac aattaaatta caaacatcag
       61 gtgatacgtt aaaaattcta aatataaaat ttacctcatc tgtacctttt tctctattga
      121 ttatcatttt tatttttctt tatatgtttt agccattttc tccaattcct tccaagtata
      181 agtatcatta tctgcacctt gctttccctc tttcattttt gattgttagg tcgtctggat
      241 ataaccaagg tcaatttcct acatcttcgt atgttttaat ccatttatct ttatcgctca
      301 atttccaaag aaaaataaaa aacaatataa aaaaggcaga tatacaaata cctacttttt
      361 gttgtctcat ttttaacgga acactatcaa tataactaac cataggaaag cgtttattgt
      421 atttgattgc aatggtatca atgtcacatt tttcaaaata ttcaataagc attttataac
      481 tgtcttgata cgttttttcg tcaacttggt atctgtaaga tatagtaata tgtgttgagt
      541 ccctaattat tcttgtgttt gcattttgag cctcattcat ttcaaaaatg ttccctaatc
      601 catttaatgc gattagtttt gaaaaactat acattaggcc tatactccaa aaagcaacgc
      661 ctgaaatgaa aaatattttt gttattgatt tcatttttcc ggagtttaac ttatcttggc
      721 ttgtaccaat tggacttaat gttaatccat tttgtctctt tactccgttt ttgcaactca
      781 ttttcattgg caaatctgac taatgccaac gtcgcaacat ctttcacata aacaaataca
      841 tttccagctg tatctacatc caggcgtggt atcctataca tcataaacgt ttgtgccaat
      901 tgttggagtt ttagagtgtc ggcttcaaca tcgtattctg tcatccattt tgcttctttt
      961 acctggtagg ttcttttgtc caacgttaca acataatatc ccacttttga agtatctcta
     1021 accaaatgtg gagcatctac gacaataaca ggatttcttt cgctatctcc acctcgaata
     1081 aagagagaaa catttttaaa ttgagaaaaa tcctcaaatt catatttggc aagaaatgct
     1141 tttttatcga aatatgccca acattcgcca tacaaaccca aggcaggtct tgctattaaa
     1201 acaagtccga ttaataccgc aataccaata gttattgttt taattatttt tttcattgta
     1261 atatttttat tcaataacca aaacctttct tttatagcga gttgatgttg agaattttta
     1321 tgaaataaag gtaatcaata attattattc ccggaaaata tatcttgaaa taggtttccc
     1381 atctttcaat atattcgtct tacacatcgt tttattgact atctttatga tttggagtct
     1441 gattttactt accaattaga aaaaatatga ataaatttct tcgtttatta gttatattgt
     1501 tattgatgtc tgcaactcat ttatcggctc aaaattcacc tctatattat gtgaatccca
     1561 tggttggcac aaaaagcatg ggacatactt ttccgggagc atgcgccccc ttcggtttgg
     1621 ttcagttaag ccccgatacg gaaatgattc ctcacaatat tgatggagtt tatcagagcg
     1681 atgcatataa atattgtgcc ggttatcagt atgatgataa aaccattgta gggttcagtc
     1741 atacccattt caacgggact gggcattccg atttgggtga tatactgata atgccgatga
     1801 ccggcgaggt gaaattggat atgggaacgg aagataatcc tgaaaccggc tatcgttcac
     1861 gatttagtca cgattatgag aaagcggaag caggttacta ctctgtcctt ttgcaagact
     1921 atgggattct tgcagaaatg actgcaacag aaagagtagg agtacatcgc tacaccttcc
     1981 cgcgagacac gaaaaccgga catatcattc tcgatttgga ttacggaatt tacaactatg
     2041 acggtaaagt gctgatggca aacctgcgcg tggaagatga atacaccctt accggttacc
     2101 gaatcacgcg cggatggtcg agaatgaatt acactcattt tgccgtgaag ttttcaaaac
     2161 ctatccaaaa ctatggttgt agtaacaaag aaaaagtgct ctataccggc ttttggcgca
     2221 aatttgatat gaccgacaac tttcctgaaa tgtttggcaa acacctgact gcctatttcg
     2281 atttcgattt ttccgacgga gaacctctcg acattcaggt tgcactttca cctgtggatt
     2341 gccgtggtgc aatgaagaac ctggaggctg aaaccggcgg aaaatccttt gatgatattc
     2401 gcaatgaaac tcagcggaaa tgggaaaaag aacttaactg catccaattt gaagccgatg
     2461 aagagacaaa atatgttttt tacacggcac tctatcatac tatgataaac ccatccgttt
     2521 atcaagatgt ggatggaaga tatagaggca tcgatcacaa tattcatcaa gccgaaaagg
     2581 gagcaaccaa ctatacggtt ttatcggttt gggatacgtt tcgtgcacaa catccgctta
     2641 tgaatcttat aaaaccttca caaagcaccc aatttgtcaa ctccatgtta aatcactata
     2701 aacaaagtgt acataaggct ttgccggtat ggtcgcacat gggaaacgaa aactggtgta
     2761 tgatcggtta tcacagcgtt tcagtggttt cggatgcaat tgccaaggga ttggatattg
     2821 atacagaatt ggcatttgaa gcttgtgtaa acagctccaa tgtcgactat tttgatggta
     2881 cgggcgatta taaaaaatat ggatatgtgc cattggaaac aagttcctcg gcagcctcta
     2941 taaccttgga atacagttat gacgattgga ccattcacaa gctggcaaat cgtttaaaca
     3001 aaagtgaaat cagtcaggag tacgctcggc gtgccttaaa ttaccgcaat atctttgacc
     3061 cttccctgca ttttgcccgt ccgaaactaa aagatggaac atttaaagag gattttgatc
     3121 tgctggatac acatggacaa ggttttattg aaggaaattc gtggaattac tccttttttg
     3181 taccacacga tgtgaatgga ttgataaagg aaatgggggg cgagaaacaa tttattcgcc
     3241 gtcttgattc gcttttcacg atgcatcttc ctgccaaata tttcgaaaag accgaagata
     3301 tcaacgagga agggctgatg ggtaattatg tacacggaaa cgagccaagt catcatgtag
     3361 cctatttgta caaatggaca aaggagccct ggaagtcgga gtaccgtctc cacgaaatca
     3421 tgcggcagat gtacaaaaac aaaatagacg gattgtcggg taatgacgat tgtggtcaaa
     3481 tgtcggcttg gtatatattt tcggcactag gattttatcc cgtttgtcct ggctccgacc
     3541 aatatgtaat cggagcaccc aagacaaaag aagcaaccat ttatttggaa aacggaaaaa
     3601 catttactgt caaaaccgaa aatttatccg ataagaatgt ttatgtgaaa tctataaagc
     3661 ttaacggtaa aaaatatgaa aagcatttca taacacacga agatattctc aaaggtggtg
     3721 aaatgctatt tgaaatgagc aataagccaa acagaaagtc cgcttcctat gaaaagcctt
     3781 attcttttac aaaatgacga tttcaaagaa gcgtaacttg tattatctgt tccacaccaa
     3841 actctaaact acggacccgg aacttaactt tatttcaata ttttgtacgg taagccttta
     3901 gcttttagtt cttcacctaa tgtgaaaagt tgcgaagtgt cttgcaagtt gaaggtaatc
     3961 gtaacctgga cgtgtccgac aggaacgttt gtaatggagc gctcgtgctc aatatcgtga
     4021 attgaagcgc gcagtttttc caaaatgctg attattttac tcaacatgcc ggcctggtcg
     4081 ggaattaaaa ctgctattct ggctcttaat ccttctccta taacaccttt ttcgataatt
     4141 tgttccaaaa tgctcgtatt aatattacct ccgcttataa ctgcaaccac atttttattt
     4201 acaacatcta cctttttgtt taagattgcg gctaaggagg cagcaccgga gggttccgac
     4261 aagtttttag cacgctgtag caatctgtag caggcctgtg ctatttcatt gtcgtttaca
     4321 accacaacat catcgacgta ttttttaact atttcgaaat ttagattacc cggagcttta
     4381 actgcaattc cgtctgctat cgtatttccc gacacaaaag gagtaagcac accattgtca
     4441 agtgagaccc gcatgctatg agctccttca gcttcaactc caataatctt gatgtttggg
     4501 ttcatgcttt ttaaagcaat ggcaattccc gaaataagtc cgccaccgcc tatgggaaca
     4561 aaaaccatat caatgtcatc caactgctca aatatttcca agccgatagt accggctcct
     4621 gatataatat cggggtgatt gtatgggtgg acaaaaacag ctccggattc gtcaagatat
     4681 tccatagccg cttcgtaagc atcgttgaaa gaatctcctt ttaaaataac attagctcca
     4741 taacttctgg ttgcgataac ctttattgga ggagcaaatt cgggcatgaa aatagtgctc
     4801 ttaacaccca attttgttgc agcataagct acgccttgcg catgatttcc tgccgaagca
     4861 caaagcactc cgcgttcttt atcttcggga cttagccggc tgatatggtt gtaagccccg
     4921 cgtattttga acgaaccggt agattgcaag ttttcaagtt ttaaataaac ttgcgcattc
     4981 accatgttag agaacgattt gctgcgttcc aaatcggtac gtttcactat tttttccaaa
     5041 ttctttttcg cctttattat gtctgataat gcaggcatag gcatgtcgca tgtactcatt
     5101 tctattgttt ttttacaaat ttacatctat ttattccaat ataaaaagcc tttaataatg
     5161 tataatcgtt cggttgtaga taccgacatt attttttcaa tttttcatct attatactac
     5221 ctattataca cgataatgca aagccgactg aaaagagtat ggcccaccta atttccgttc
     5281 ccggcttaaa cactagatag agtgtaatgg ctatactata acagaaaaag aagagtgcaa
     5341 gccttttctt tactgactca gggattttca ttctaaaaga tatttcttat tacgaaaaca
     5401 aaataaccaa gtcgcaccga ttgttctact gtagagattt gaccaacttt gtaatacgct
     5461 ccatttcgtc agcaatcttc aggcgctggg ggcagctgcg ctcgcactta ccacagagtc
     5521 tgcatttgtc tgcacgctcc tcttcgggta tggcatagta accctccagg aatatcttct
     5581 ttctgcgctc aaactcttca ggagtctggc tgtcacgctc aggaatattg ctctcattta
     5641 cacatttgtt gtaatgcatg aagttggccg gaatatccac tccaaattta cagggcatac
     5701 aatacttaca agccgtacac ctgattggac gggtcttcaa gaaaatattc agagctttgt
     5761 cgagagctac acgctcctta tcgttgaggg acttaaattc ggtagagaga gtattgacat
     5821 tatccaccac atgctccata gcgctcattc cgctcaacac agtgagcact cccggtagcg
     5881 aagccacata ccttagcgcc caggatgcaa tactcttttc aggatgtacc cttttgagta
     5941 ccgcttctgc atcagggtta agtttggcga gcattccacc acggatgggc tccatcacga
     6001 tgcaggggat atccttctcc tcaagccttc tataaagata cttggcatcc atctggtccc
     6061 aatcatggta gtttatctga atttgtacga aatcccaatc atatttttcg agcatcttgt
     6121 caaacaactc atttgatccg tggaaggaga atccgagatt acgaatccta cccgcctttt
     6181 tctcctcgat aagatattcg agggttccga tttcctcata gacacgcaca taatcctctt
     6241 cgcggcttac tgagtggagc aaataataat caaaataatc cacctggcag cgctgcaact
     6301 gggtttcaaa tatttccttt gccttttcaa gagatgtcac catccatccc ggcatcttcg
     6361 aagcgaggta gaaactctca cgcggatatc gctttaaagc cttgccggta aaaatttcgg
     6421 atgtgccgcc gtgataatta aaggcggtat caaagtaatt gaccccatgt ttataggcat
     6481 agtctatcat actgagtgac aactcttcat cgatggactt gtcggaacgg gtcgggaagc
     6541 gcatcattcc gaaccccaag agagaaacca tattggaact ccctttgtgt cggcgtttag
     6601 ccacattcgg ctctctcgac tctacattgc gggctgaaat ggaactgaga ccggttgtgg
     6661 ccatagccac accgattatg ccggtaccga aaagcttcag tgcctcgcga cgggaaagtt
     6721 ggttcttctt tttatccatt tgtataaatg tttgaaaata atttgaaata ttttgaaata
     6781 atttgcaccc tttttcacaa ggattatatc gtacaaaaat agatttttca tctgagaagt
     6841 gcaatagtct gaaaaactta aattttaagg aacagaccaa tatcgctttt cgggatttgg
     6901 taaatatttg cttttaagtg tgtataaatg gctcaaggtt gctcagagcc gcagcgaaat
     6961 ctatttttgt cattagtaac tcacgactta aaggtgataa aaagagaatg ttgtgcaaaa
     7021 cttattgtgc cacctgcata ggaataacgg cagtaacggt aaccgtggag gtggatcttt
     7081 ctaatggcat aggtatacat ctggttgggc tgccggataa tgcagttaag gagagtttgc
     7141 tgagggtaac tacggcactc tcgacttatg gatacaggat tcccgggcgc aaactggtct
     7201 tcaatctcgc gcccgccgat atacgaaaag agggttcttc ttatgatctg gctatcgcaa
     7261 tcggactgct ggcggtgtcg gaacaggcat atctgcccgg atgcagcaaa tatatcatgc
     7321 tcggtgaact ctcgctggac ggtcgcatac gcaatgtgac cggtgcactt cccattgccg
     7381 aacacgcccg cgaaatggga ttcaaagggt gtatttttcc gaaggaatcc gccttggaag
     7441 ctgccgatgt agagggaatt gacatatatg gcgtaagtat gctaagtgag gtattggaga
     7501 tactgcgcgg agataactgc ccccatctgc tggtggtacg cgacccggaa acatccaggc
     7561 gaccccatta tgctgaggat tttaagaatg taaaggggca aaggatggct aaaaggggtc
     7621 tcgaaatcgc tgctgccggt ggccataatc tcctgctggt aggcacaccc ggcagtggta
     7681 agaccttcat ggcctcctgt ctaccttcga tactgccaca aatgagcagg gatgagtcca
     7741 tagagaccag caagatctac tctatttccg gtaattctac gggcagacgg gggttgatgc
     7801 tagagcgtcc ttttagaagt ccccaccaca gcgccagtct ggtctcgtta atcggaggag
     7861 gacagaacgc ctctcccgga gaggtttccc tttcccataa cggcatattg tacctcgacg
     7921 agatggcgca gttcagcaag ataatactgg atgcactgcg ccagcccctt gaggacggca
     7981 aagtcaccat ctccagagct cgctataaag ttgaatatcc ggcatctttt atgttgatag
     8041 cctcaatgaa tccctgtcca tgtggttact atggtgacga ttcgcacaaa tgcacctgca
     8101 cacaatcggc cgtgatgcgc tatatgtccc ggatttcggg accgatgatg gatcggctcg
     8161 atatgcatat tttcgtcaaa gcggtcagcg gcgacgatct cattgcagag ggagaagaag
     8221 aaaccagcga agctattgcc gcaagggtaa tggcagcacg gcaaatacag tacgaacgct
     8281 tcagtggtga agggatattc acaaattccc agatgaacgt gcggcaactc aacaacttct
     8341 gcaaggtaag caccgaacag aaacgctttc taagggatgt aattaaccgg ctcaatctgt
     8401 cggcccgctc ctatagccgt atcctcaaat tgtcccgtac aattgcagat attgatggcg
     8461 ataattttat atctttgcag catatcagcg aagcgataca atttcgcaat ctggacagag
     8521 gcaattttta tgattgagat tatcatcaac ggtaccggag accttcagag agctgcacgc
     8581 gtctttctcg aaaaggcggg tgaaagcagg ataatcgctt tttacggctc aatgggggca
     8641 ggcaaaacca cattcattac tgccctatgc cgtcaactgg gagttttgga tgtagtcaac
     8701 agtcccactt tcacaattgt caacgaatat gcagatgcgg aagggaatcg tttttatcat
     8761 ttcgactttt accgtatcaa ccgcttgtcc gaagcgatag atattggcct tttcgagtat
     8821 ttcgattcgg gtgattattg ctttatcgag tggcccgaga tgatagagga acttctgccg
     8881 gaggagaccc tcaaggtcaa tattcttgtg gatgacgctg ataccagaag actattattc
     8941 taaaattcta ataccatttt actatgagac gatttctttt ctcaattgca gcaattttgc
     9001 ttgcagtctc tatttgccaa tgctctccaa aaggtcagaa tgatgaaacg gcgatggatc
     9061 tgcctcaaga gcagattctg gtgggcgccg agtcacttga cgaatatttg cctttgctgg
     9121 aggggaagaa ggtaggtctg ctctccaacc agacaggcat actctccaac ggaactcatc
     9181 tgctggacac cctcctctca catggagtta acatggtttt aattctctcc cctgaacatg
     9241 gtttcagagg caatgctgac gccggcgaac tggtgagcag ttcagtcgat gagaagaccg
     9301 gtatacccat ccgttcacta tatgacggca aagggggcta tcccacacag gaaacaatgg
     9361 atgagatcga ggttatggtt tttgaccttc aggatgtcgg ctgccgattc tacacctatg
     9421 tcactaccat gacgaaaaca atggaggtat gcgccaaaaa tggcaagaag atgatcataa
     9481 tggatcggcc caatccgctc ggattctatg tagacggccc catcctcgac atgcaataca
     9541 aatctacggt aggcttttta cccatacctg tagttcacgg tatgactctg ggagagattg
     9601 gtatgatgat aaatggtgaa aaatggctgc ctgatggcat ccaatgcgat cttaccgtca
     9661 ttaaatgtaa aaattatact catcaatcac actattctct tcctgtaaaa ccttcaccca
     9721 accttcctga tatgcgttcc atatatcttt acccctctat gtgctatttc gaggccactc
     9781 ccgtaagtct cggacgtggt acagataagg ctttccagat gtacggacac ccaaatatga
     9841 agggatacaa atattcattt acaccgcgca gcgtggatgg agcaaaaaat cctcctcaac
     9901 tcgacagaga gtgcttcggt gttgaccttc gtactgagcc atcgattgag caaatcaatg
     9961 ccgtaggtct caaccttgaa tatgtcatag atgcataccg caatctcaat ctcgactcac
    10021 acttcttccg ttcattcttt gaacttctga ttggccgtga ttatgtcaga aagatgataa
    10081 tgtcgggagc ttctgctgaa gagattaagg cgatgtgggc cgaagatgtc gcctctttca
    10141 aagagcaaag acgaccctat ctgctatatg aagaataaag ttgtgttgcg gggtgcgagg
    10201 tacgggttgc gaggtatttg gttctctgtt ttcaatgtgt gacatggagt attgatccac
    10261 accgagtacc aagaaactcg gaactcagaa ctcagaactc agaactcgga actcagaaat
    10321 aatagagaag cgaccctaaa agatcgcttc tctattattt atgtatatag gttatgacgc
    10381 tacatcctga atctcaggcc caactccaga ttgaactgta tcggctgctg ggtacgtatg
    10441 ctgagcggct gattattatc aaaataatag gccaatgagg gcttgaatgc gatacctaca
    10501 tgtttgacaa accagtattc cgcacccaga cccaagttta ccgaccactg tacgccgtca
    10561 actttttctc gtctatccgt gctgccataa acatagcgcc tcatgataca cttatcaacc
    10621 atacctccta ccgatacaaa tgcgtcaaac ctcgggcggt cgacaaaatt gtaggagaga
    10681 ttgagtggaa tgccaaggta atggagctgg cttgagacat ttggatgcct ctccagattt
    10741 atcatggcac tatactgcga atgaaggtaa gtgtagacaa ggcctgatcc gatatataat
    10801 ctcggcacta tggcttgttt aaactggaga ctgaacgaca gcggaatgtc aaactgcgga
    10861 tcatcatcga aggggataat actctgggac tcaataggag tgcccaattc tgacggagca
    10921 tatctcggcc caaggtcggc aaatattcca tccttcgatg caacttgtga aatattggaa
    10981 gatatagcga gtaatgatgt gttcttagaa atacggcgca caggcttttc ttgaccgaga
    11041 atcctgtcga gatctttttc gtcccattca cggactacag gatccgaagc aggcttatcg
    11101 tcctgtgcaa ctacctgctc tgccaccact tcattgtctg atacaactac atctacatcg
    11161 gcaacaactt tctcctcagc aactttgtca actgtagtct cagaaagttt atctgcaacc
    11221 ggtgttgcag tacgggcaac aggttttgta tgcttggcca ctgctcccga tggggtaagg
    11281 tcggcaactt gttcggccat tggtctaatt tcccgatcag ccggaatccc ggtatcaggg
    11341 atagtggtct ctgcaatggc cgaagggagg gaaggaagtg tagaaatctc atctccccta
    11401 aatataaata atccggcagc aagtaccgct gctgctgcaa ccgaaatgaa cgaaacacgg
    11461 cgcaccacca tcatcctgtg acggcgtgcc atcttgctac gaataccatc ccaagtggat
    11521 gcatcaggca tctcaacgct ggcatcaagc ttttcccgta atagttcgta tagatctttt
    11581 tcgttcattc aatataattc ttcaatcctt ttctgcagcc attccctggc tcttgagagt
    11641 tgcgacctcg atgatgctga agatatgccc agcatccggg cgatctcgtc gtgttgaaaa
    11701 ccgtcaatgg tgagattgaa aaccgcgcgg tagcccggag gcatactttc aatgagcgct
    11761 gtgagctctt tcgcccccat cctggaaacg gcatcttccc tactttccat cataaatgcc
    11821 gcaggaatgt tgtcaatctt gtctgcatac ctgagggcat catttttacg aagcgccatc
    11881 aatgcttcat tgacaaaaat ctttctcatc caaccttcaa aagaaccttc tcccttgtaa
    11941 gtacggagct ttgaaaaaac tgtaacaaag ccgtcctgca agacatccat ccccgcttcg
    12001 cgatcgccca tatacctgat ggataaagcc agcatctttg gcgcgtaagt gtcgtacaag
    12061 cgcttctgag ccactacatc ccttttcaaa cacctctgta tgagatcatt taaatcagta
    12121 tcagttaaag ccgactgctt cttcattgac tgtttcatta tatagatgca aaaatagcac
    12181 aaaatgttgc atcaggtgca aaatattttt ggagtgattt ttgtttgaac atttatactt
    12241 aattttgcat attgtaaaaa tataaaggaa tgtgagaaag atgaaaaaaa ttctaataat
    12301 ttttgcttta acaatcgtta attttttgtg cgcagcacaa tcacacacct accttgatgc
    12361 attgcaagcc tatcaaaagg gtgaaatagc tcaggctcat accctttttg taaacgagat
    12421 aaaggcaaac cccgataacg atgccgccca cttttatctg gcgatgatct attctgcgaa
    12481 cacgaacagt catataaaag cggaagaaga attcaagaag gctctcgcca ttgatccaaa
    12541 caactattgg tataaatatt cccttgctct attttatgct gaaacggata ggatggagct
    12601 tactactctg ctgcttgaag agctgattga ggcctttccc aaaaagagtg agctctattt
    12661 tacggcaatc aatgcatttc tcagccagaa tgatatcacg aaggctctta ccacactcga
    12721 caaaatagag gggaagatgg gtaaaaccga gatgataggt atgaccagaa tggacctgat
    12781 gagtaaacag ggaaagtata ccgacaaaga actctacgat taccttgaga gctattataa
    12841 ggagtgtcaa agtccgagaa tggccacaat gctcggcgat cgctatgcat cactctataa
    12901 cgactctctg tcactcttct attacaatca ggccattgat atagatcagg gatattctcc
    12961 ggcctattat ggtcgtgcac atgtttttca ggcactcaga caatatgagc gatattttga
    13021 ggatatgggt atttttatga aggatcctgc catctctccc cgcctcaagt ccgaatacct
    13081 caacaaactg accgagtctg cacaatttgt aatggcattt ccggaggaga cagaacagat
    13141 ggtactcgag gcatacacga cacatcctaa agatgaggcc atcaccacta tggccggagt
    13201 ctttttctac agaatggata agactcacta tgccatagaa ctgatgaagc agaattcgga
    13261 aaatcaccct gataatcctg aggctgcctt acagtacctg atgatgctct actaccagaa
    13321 attgtgggac gaactgatcc ctgcatgtga ccgcactttg gagcgtttcg gctacaattc
    13381 cgacatagtg cagctccgag gcatagccta tagcctgaag gaagagtttg atttggccat
    13441 aaaggacttc aaacttatag catcttcggc accgaaagat agtgccacaa ttgtcttcac
    13501 caactcaatg ctgggcgatc ttcatcacca aaagggagat agcaaaaatg cctataagca
    13561 ttacaaaaaa gtactcaaga ccaatcccaa ctatgcccca accctcaaca attacgcata
    13621 ctacctcgct ctggaaggta aaagcctcaa aaaggcgaaa gctatgagcg aaattaccat
    13681 aaagcaggag ccggataacc ccacctacct cgacacctac gcctggattt tacaccttct
    13741 cgggcagaac atagaagcca aggcgatttt taaacacgcc atgctctacg gaggcaagga
    13801 gagtgccgta atcctggatc actacggtga cgtgctattt gaactcaaag agtatgatct
    13861 tgcctttgtc tattggaatc aggcaaaagc gctcgatgac accctcggca tagaggaaaa
    13921 aatcaaagtt aagaaggcag aaatcggcag ataaccatga aacgtctcct gctaatactc
    13981 ataattctac tcccctctct gagcctctcc gcacaggata tctcaaggca gtcagagcag
    14041 aaaaagcggc ttgaagaaga gattgagttt atcaataagc aacttaagga gatcctatca
    14101 aaggaaaaag ccagcacctt agagctcacc ctcataacca gaaaaatatc ca
//
LOCUS       DAOSIE010000009        13465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_4674_length_13465_cov_4.060030, whole genome shotgun sequence.
ACCESSION   DAOSIE010000009 DAOSIE010000000
VERSION     DAOSIE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13465
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4674_length_13465_cov_4.060030"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLI69_00735"
     CDS             complement(<1..542)
                     /locus_tag="PLI69_00735"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HOG41049.1"
                     /translation="MVVAATGFFDGLHLGHRKVIEKLTSIAAEQGGESMIISFWPHPR
                     NVLQQDAYKLRLLTTLEEKRALCFSLGIDRFEVICFDKEFSQLSTEEFFRGYLIEKYK
                     VSTLVVGYDHRLGHGKDERNIEEIAASCGIRTVRVGKFQRGERVISSTAIRNCLLKGD
                     IACANEWLGYRYSLHGVVISG"
     gene            complement(533..4768)
                     /gene="glgP"
                     /locus_tag="PLI69_00740"
     CDS             complement(533..4768)
                     /gene="glgP"
                     /locus_tag="PLI69_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOG41050.1"
                     /translation="MKQNSIMPDYLFEISWEVCNKVGGIHTVIATKSLFLGKEFKKNH
                     IHIGPDVWRDIAQNPEFNEDPMLFRSWREAALSEGLRVRVGRWNVPGNPIAILVDFTQ
                     FISRKDEILTDFWKNFGVDSLNGNWDYIESALFGYAAANVIEHFVRFNVTPNKRVVAQ
                     FHEWMTGSGLLYIKQRELPIGTIFTTHATVIGRSVAGRNLPLYDQLSNLNADDIAREF
                     GVVSRHSLEKAAALNANVFTTVSQITAMECKQFLGREVDVITPNGFEKSITPPEEQFP
                     EYEARARKKLIEVASIMSGEQYGEDALLICISGRYECTNKGIDIFIDALSRLNSGGYK
                     GKPVLAFIMVPSGHNGPDKELLAKLHGADSNYTTNVSHLLSDDYDMVVNRLRALKLNN
                     SSGDKVKVFFIPTYLNGSDGIFDMSYYDLLVGMDLTLFPSYYEPWGYTPLESLAFKVP
                     TATTTLAGFGLWVQEHYPKDKSRKSIDIITRTDSNYDDVVNDVVNRIREIAALSPAEK
                     KSYKENAKEVSEIALWENNIKYYKRAFSLAIEKLVEAKGEFLGIVDDMPQSYNKLTTN
                     DPFWTNVLVNRELPQSLRHLDTLARNLWWCWNQDAIDLFISLDPKLWEKCKGNPISLL
                     DKIDLKRYKELENDSAFLARLEAVYADFHAYMEGKRHRKGPKVAYFCMEYGLDTSLKI
                     YSGGLGILAGDYLKEASDMQVNLTAVGLLYRYGYFTQHLSAMGDQISEYEPQNFMKIP
                     ATPVRDSAHNWVTVKIAFPGRNLYARVWRVDVGRTELYLLDTDFEDNRPEDRQITHQL
                     YGGDWENRLKQEFLLGVGGIRALRALGKDAEVYHCNEGHAAFIGLERLYEYISKENLS
                     FPEAMEVVRASSLFTTHTPVPAGHDAFTEDMLRAYIAHYPDKFKIDWKTLMGLGKMDV
                     DNPSEKFSMSVLAANLSQEVNGVSWLHGVVSQKIFSGMWPGYLPEELHVSHVTNGVHY
                     PTWTAPEWKEVHAEVFGEEFKTHHYDKSCFDGIHKVSDEKIWSIRQQLRSKLIKAVKD
                     IMSDTAATSHYSPHQIVKIKKTLRDDILTIGFARRFATYKRATLLFSNLDRLNDIINN
                     PDRPVQFLFAGKAHPADKAGQDLIKRIIDISKMPQFIGKIVFIPNYDITIAKLLIPGV
                     DVWMNNPTRPLEASGTSGEKAVMNGVMHFSVLDGWWVEGYKPGAGWALPMERTYDDQQ
                     FQDELDSATIYTIIENEIAPIFYDKDKKGISADWIEIIKNTVSQVANNFTTNRMMTDY
                     LNQYYIPLAKRQSEMVASDFAKAREMAFWKKRLRREWPHLEIISLSKPDNTKDSLTLG
                     KEFTAELVIAIGDLTPEDLGVELIVATQNRRGEFKIRESFEFKTVSFENGIATYRANV
                     VPDMSGSFHMAMRLYAKNEKMPHRQDFELVKWL"
     gene            complement(4791..6002)
                     /locus_tag="PLI69_00745"
     CDS             complement(4791..6002)
                     /locus_tag="PLI69_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOG41051.1"
                     /translation="MKSICLYFQVHQPDRLRQFRFFDIGNDFHYFDEFANRTILRRVA
                     DKCYLPANNILLDLIKKHGKDFKVTFSISGVAVEQFLMYAPEVIESFKALAATGCVDF
                     LAETYSHSLASLSSPAEFKKQVKQHAEMITRVFGVKPTAFRNTELIYSDSIGDAVADL
                     GYNVMLTEGAKHILGWKSPNFVYTNAINPRQKLLLRNSSLSDDIAFRFSNKSWDGWPL
                     TADKYAAWLVDSLSGEDIVNLFMDYETFGEHQKEYTGIFDFLKALPEAILAKGDIRFR
                     TVPEAAAFHQPVAPLHVQYPISWADEERDTSAWLGNELQNEAFNKISALEADVLKTKD
                     EKLLNIYRKLQESDHFYYMCTKFFSDGSVHNYFNPYNTPYEAFINYMNVFSDFEIRVK
                     EKIGSLKRDKK"
     gene            complement(6014..7303)
                     /locus_tag="PLI69_00750"
     CDS             complement(6014..7303)
                     /locus_tag="PLI69_00750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG41052.1"
                     /translation="MRVLMFGWEFPPHIAGGLGTACYGLTKGLAHHNVEVLFVMPSAS
                     GDEDQSAVRIINASDVAVYDTCKNIDEFLGKVDFLRVGSNLRPYIDPISFRELVTEEQ
                     KYQTEEFKGYFKEKYKFSGKYGANLMEEVARYAMVAGAIAQQHQFDVIHAHDWLTNLA
                     GIAAKKVSGKPLVIHVHATEFDRTGENVNPEVFRLEQTGMREADKVITVSNLTRNIVI
                     NKYGIDPSKVITVHNAVDFSGRSDIEVQRGVKEKIVTFLGRITFQKGPEYFIEAANKV
                     LKMYPNVRFVMAGSGDLMNRSIKRVAQLGISDRFHLTGFLRGQDVQRMFAYSDVYVMP
                     SVSEPFGISPLEAMISNVPTIISKQSGVAEVLRYAIKVDFWDIDALADAIYGLLNYPA
                     LSKMAIDSGLEEVNSLKWDHAAAKVKRVYEAAINAKK"
     gene            complement(7306..9240)
                     /locus_tag="PLI69_00755"
     CDS             complement(7306..9240)
                     /locus_tag="PLI69_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HOG41053.1"
                     /translation="MAYLKFNKSELVNLEYSLKRELISTNMAGGYTNTTIVCCNTRKY
                     HALLAVPVDNFGGSKHILLSSLDETLIQHGRPFNLGIHNYGSVFEPKGHKYIIDFEMD
                     PIPVITYRVGGMVFRKSIIFSTKNENQLLIKYTLVDAHSNTILRLKPFLAFRNIHSLT
                     RANSDADTRYRSIENGVAFKMYEGFPDLNLQISKPCDYVPSPDWYYNIVYREEYRRGF
                     DCTEDLFVPGYFEMPINKGETIIFSASTSESNPNNLKTKFTRELNVREPQESYEDCLR
                     DAAMQLFTSRNDKIEICAGYSWLESGLLRETCVALPGLTLYNNGDVKLFRRILDDTIA
                     THNEAILKGCDQVEAPLRLTELVQHLISYTKESAKVWSKYGPILKDIIHSFIQGRPEV
                     ILHNNGLLWAQKSGVPLTWMDAYAGGRPVTERAGYQVETNCFWYNALCFASAMEKSFA
                     KENDFTRECDAIKKKIEDNFFNLFWVESRRHLADYVDGLGQNICTRPNQIYACSLDYS
                     PVSETVQDDILRSINQELITTRGVRTLSPKNPHYKGVYEGNQIERDLATHNGSTRPWL
                     LGAYIASCLKLYGASYLRTSQALVSGFEEDMNIHGIGMVAEIYDGDPPHHPHGAILSA
                     SATAEIIRVKYLINKYKLEE"
     gene            complement(9339..11321)
                     /locus_tag="PLI69_00760"
     CDS             complement(9339..11321)
                     /locus_tag="PLI69_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOG41054.1"
                     /translation="MVAMKIYKKDKYLEPYKEIIERRNAMVLQKKRQMAGEGRLSDVV
                     NNHLYYVLHRDNRGWVFREWAPNATRIWLIGDFNGWKREERYTFTPTGGGNWELVVPA
                     EIISHGTLYRWYVEWPGGGGERLPSYATRCVQDEHTKIFSAQVWAPSAPYKWKRKFRR
                     RVKNPLIYETHIGMSSEEERVAGFSEFRRDVLPRIAAGGYNTIQIMALQEHPYYGSFG
                     YQVSNFFALSSRFGTPEEFKELVDEAHRLGIAVIMDIVHSHAVKNEVEGLSRLDGSYT
                     TYFHAGERGEHPAWNSRCFDYGKNETLYFLLSNCKYWLEEYNLDGFRFDGVTSMLYYD
                     HGLERNFIGYDCYFDGGQDEDALVYLGLANILVKEVKPLAFTIAEDMSGMPGLAAPIK
                     KGGFGFDFRMSMGVADHWIKWIKELSDWDWSMGEIFYQLTNKRADERTISYAECHDQA
                     LVGDKTIIFRLMDKEMYSSMNKESENIIVDRGMALHKMIRLVTLATAGDGYLNFMGNE
                     FGHPEWIDFPREGNSWSYFYARRQWSLADADYLRYKSLGDFDKSMIHLFADNNILKYK
                     IVSLRQDEEKKILIFTRGAFLFVFSFNPEHSFENYCFEAPAGKWVPILDSDAPEFGGF
                     GRNDAGVEHFTRYKEGQNLLSLYIPCRSALVLRYDD"
     gene            complement(11299..11511)
                     /gene="xseB"
                     /locus_tag="PLI69_00765"
     CDS             complement(11299..11511)
                     /gene="xseB"
                     /locus_tag="PLI69_00765"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOG41055.1"
                     /translation="MKEKMSYEEAFAELERLVVGIEDPERDLSGIENDVKRAMELIEY
                     CRSAIRGEQEKIERIINERNGSDENI"
     gene            complement(11508..12707)
                     /gene="xseA"
                     /locus_tag="PLI69_00770"
     CDS             complement(11508..12707)
                     /gene="xseA"
                     /locus_tag="PLI69_00770"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOG41056.1"
                     /translation="MDDGKLYTLYQLQSDISEVLAEAFTDSLWVRSEVSECSVNRSGH
                     CYLTLVEKEEKSGRLLAKCSAVIWAQTYRILSAHFRSVTGSDIVAGMNILVKVQVNYS
                     ELYGLSLNIRDIDPSFTVGNLELERQRTIARLSEEGMMDINSSLELPALPRSLAVISA
                     ETAAGYRDFMEHLHGNQYGFTFATTLFPAPMQGAEAPSGIIAALDTIAQRIDEFDAVL
                     ILRGGGSVMDLVCFDDYELALNVAQFPLPVLTGIGHDKDYHVIDMVAWHNLKTPTALA
                     DFFIEMFVHEEQAVAALISRLKLALKSKSHRELALFDNMKVRIRSAVSLKASEALRRV
                     DLLQTRLIAANPRTLIEKGYLVVLKDGRRIATGAELVPGERLKVMFSDAVVECEVVSS
                     EIKNNAI"
     gene            12794..13402
                     /gene="yihA"
                     /locus_tag="PLI69_00775"
     CDS             12794..13402
                     /gene="yihA"
                     /locus_tag="PLI69_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOG41057.1"
                     /translation="MIIKKAIFAGSSERVAQKPKALYPEFAFIGRSNVGKSSLINSLC
                     QNKKLAHTSSTPGKTQLINHFLINDSWYLVDLPGYGYAKISKTARAKLAKIIDGYIDL
                     SQEMILLFVLLDSRHDIQQIDLDFLISLGERAIPFSIIFTKCDKSPKNVLEKQIEKNR
                     NQLLEYWEELPPIFLSSSVTGSGREEILSYIDSILSTLNSEP"
BASE COUNT         3612 a         3283 c         2882 g         3688 t
ORIGIN      
        1 ccggagataa ctactccatg aagagaataa cgataaccaa gccattcatt tgcacaagcg
       61 atatcccctt ttagcaaaca gttgcggata gcagtggaac tgatcaccct ctctcctctc
      121 tgaaatttcc caacacgcac cgtacgaata ccgcaggagg cagcaatttc ctcaatattc
      181 ctctcatctt tcccatgtcc aagacgatgg tcatatccca ctaccagcgt agaaactttg
      241 tacttctcta taagataacc tctgaaaaac tcttctgtgg ataattggct gaactctttg
      301 tcaaaacaga ttacctcaaa tctgtcaatc cccaaagaga aacacaatgc gcgcttctct
      361 tcgagggttg tcagtagtct gagcttgtag gcatcttgct gaagtacgtt tctcggatgg
      421 ggccagaaag agataatcat actctctcct ccctgctctg ctgcaatgga ggtgagcttc
      481 tctatgacct ttctgtggcc taggtgaagt ccgtcaaaga aacctgtagc cgctacaacc
      541 attttaccag ttcgaaatcc tgcctatgag gcatcttctc atttttagcg tacagacgca
      601 ttgccatatg gaaagagcct gacatatccg gaacgacatt ggctctatat gtagctatgc
      661 cattctcaaa ggaaacagtc ttgaattcaa agctttcacg aatcttgaat tctcctcttc
      721 tattctgagt tgccactatc agttcgactc cgagatcctc aggagtgaga tctcctatgg
      781 caataaccaa ttccgctgta aattcttttc cgagggtaag tgaatccttg gtattatcgg
      841 gcttggagag tgaaattatt tccagatgag gccactccct gcggagacgc ttcttccaga
      901 atgccatttc cctcgctttt gcgaaatctg aagcaaccat ctcgctctga cgtttcgcca
      961 gaggaatata atactggtta agataatcgg tcatcatcct gttggtagta aagttattgg
     1021 ctacctggga tacggtattt ttgatgattt caatccaatc tgccgagatg cccttcttgt
     1081 ccttatcata gaagatagga gctatctcgt tctctatgat ggtgtaaatg gtagcagagt
     1141 ccaactcatc ctggaattgc tgatcatcgt aggtgcgctc cataggaaga gcccatccgg
     1201 ctcccggttt gtaaccctcc acccaccatc cgtcgagtac ggagaaatgc attactccat
     1261 tcataacggc tttttcacca ctggtacctg aggcctccag gggacgggta ggattgttca
     1321 tccatacatc gacacccggt attagaagtt ttgcaatggt gatatcatag ttgggtatga
     1381 atactatctt gcctatgaac tggggcatct tggaaatatc tatgattctc tttatcagat
     1441 cctgacccgc tttatctgcg ggatgcgctt ttcctgcaaa gaggaattgt acggggcgat
     1501 cagggttatt gattatgtca ttgagcctat caagattgct gaaaagcagg gttgccctct
     1561 tgtaggttgc aaagcgacgg gcaaatccta tggtaagaat atcatcgcgc agagtctttt
     1621 taatcttgac aatctggtga ggagaatagt ggctggtagc ggcagtgtcg ctcattatat
     1681 cctttacggc tttaatgagt ttactgcgca actgctgtct tatactccag atcttctcat
     1741 ctgatacttt gtgtatgccg tcaaagcaac tcttgtcgta gtggtgagtc ttaaactcct
     1801 cgccgaaaac ctctgcgtgg acctctttcc actcgggagc cgtccatgta ggatagtgaa
     1861 ctccgttggt tacatggctc acatgtagct cttccggcag gtaacccggc cacattcctg
     1921 aaaaaatctt ctgggaaacc actccgtgca accaacttac cccatttacc tcctgagaaa
     1981 ggttggcagc cagtacgctc atggagaact tctctgaagg attgtcgaca tccatcttac
     2041 cgagtcccat aagagtcttc caatcaatct tgaacttatc gggatagtgc gctatatatg
     2101 ctctaagcat atcctcggta aatgcatcgt gtcctgcagg gacaggagtg tgagttgtaa
     2161 agagtgagga ggcccttacc acttccattg cctcagggaa ggagagattt tccttagaaa
     2221 tatattcata tagacgctcc aggccgatga aggcagcatg gccctcattg cagtgataga
     2281 cctctgcatc tttaccaaga gccctgagtg cccttatgcc gcccactccc agcagaaact
     2341 cctgtttaag acgattttcc cagtcaccgc cataaagttg gtgagtgatc tgcctatcct
     2401 ccggacggtt gtcctcaaaa tcggtatcaa gcagatatag ttcggtacga ccgacatcta
     2461 ctctccaaac ccttgcgtat agattacgac cggggaaggc tattttaacc gtcacccaat
     2521 tgtgagcaga atcccttacg ggagtagcag gaattttcat gaagttttgc ggttcatatt
     2581 cggaaatctg atctcccatt gccgaaaggt gttgggtgaa gtatccgtat ctgtaaagca
     2641 gaccgacagc agtaagattc acctgcatat cacttgcttc cttgaggtag tcgccggcaa
     2701 ggatacccaa tcctccggaa tagatcttta gcgaggtgtc caaaccatat tccatacaga
     2761 aataggccac cttcggaccc ttacgatgcc ttttcccctc catataggca tggaaatcgg
     2821 cataaaccgc ttcaagacgc gcgaggaaag cggaatcatt ctccagctcc ttatatctct
     2881 ttagatctat cttgtctagt agagatatgg ggttgccctt acatttttcc caaagttttg
     2941 gatctaaaga aatgaagaga tcaatggcat cctgattcca gcaccaccag agatttcttg
     3001 ccaaagtatc aagatgtctg agactttggg gcagctccct attgacaagc acattggtcc
     3061 agaaaggatc gttggtggtg agcttattat atgactgggg catatcatcc actatgccga
     3121 gaaattcacc tttggcttca accaactttt caatggcaag cgagaaagct ctcttgtagt
     3181 actttatatt attctcccac agggcaatct ctgaaacctc ctttgcattc tccttgtatg
     3241 actttttctc tgcaggagaa agggctgcaa tctcacgaat gcggttgact acgtcattta
     3301 caacatcatc atagttggaa tcggtgcggg tgattatatc tatcgattta cgactcttat
     3361 ctttgggata atgttcctgt acccaaagac caaatcccgc aagggtagta gttgcggttg
     3421 gcaccttgaa tgcaagcgac tcgagtggag tatagcccca aggttcatag taagagggga
     3481 agagggtaag atccatgcct acgagcaaat catagtaact catatcgaat ataccatcac
     3541 tgccgttgag gtaggtaggg ataaagaaga ccttgacctt atctccggag ctattgttca
     3601 gtttaagtgc cctaagacgg ttcaccacca tgtcatagtc atcgctcaaa agatgtgaca
     3661 cattggtagt ataattcgaa tcggcaccgt gaagctttgc caaaagctcc ttgtcagggc
     3721 cgttatgccc tgatggtacc attataaagg ccagcaccgg cttacctttg taaccgccgc
     3781 tgtttagacg actcagagca tcaataaaga tatcgatacc cttatttgta cactcgtaac
     3841 gcccgctgat acatatcagc aaagcatctt ccccgtactg ctcacccgac atgatggaag
     3901 ccacctcaat cagcttcttt ctggctcttg cctcatactc gggaaattgc tcctcagggg
     3961 gagttatact cttttcaaaa ccattagggg taatgacatc cacctctctg cccaggaact
     4021 gcttgcactc catagccgta atttgactca ccgtagtgaa tacgttggca ttaagagcag
     4081 cagctttctc cagcgaatga cgggaaacaa caccaaactc tcttgcaatg tcatccgcat
     4141 tgaggttgga gagctgatca tagaggggaa gatttctacc ggctacacta cgaccgataa
     4201 cagtagcgtg agtggtaaaa atagtaccta ttgggagctc ccgttgcttt atgtagagaa
     4261 gaccggatcc tgtcatccac tcgtggaact gcgccaccac acgcttattc ggggtgacat
     4321 tgaagcgcac gaaatgctca attacattag cggcggcata gccgaaaaga gccgactcaa
     4381 tgtagtccca attgccgttg agtgaatcaa ccccaaagtt tttccaaaag tccgtcagga
     4441 tctcgtcctt tctggagatg aactgggtga agtccaccaa gatcgctatg ggattgccgg
     4501 gaacattcca gcggcctacc ctcacacgca aaccttcact cagagcagcc tctctccagg
     4561 aacggaagag catcggatcc tcgttaaact cggggttctg ggcaatgtcc ctccatacgt
     4621 cgggacctat gtgaatatga tttttcttaa attcttttcc taaaaaaagt gattttgtag
     4681 caatgacagt gtgtataccg cctaccttgt tacaaacttc ccaactgatt tcaaacaggt
     4741 agtcgggcat aatcgaattt tgtttcataa atgtattata gtggttctat ttatttttta
     4801 tccctcttca atgagcctat tttctctttg actctgatct cgaaatcgct gaatacattc
     4861 atatagttga tgaatgcctc gtagggggta ttatagggat taaagtagtt atgtacggat
     4921 ccatcggaga aaaacttggt acacatataa tagaaatggt cgctctcctg gagttttcta
     4981 tagatgttga gaagtttctc atctttcgtt ttaagcacgt ctgcctcaag ggccgatatc
     5041 ttgttgaaag cttcgttttg gagttcgtta ccaagccagg cactggtatc cctctcctcg
     5101 tcagcccagg aaatagggta ttgtacgtga agaggtgcaa caggctggtg gaaggcagct
     5161 gcctccggaa cagttctgaa gcgaatatca cctttggcaa gaatagcttc aggaagtgcc
     5221 tttaagaagt cgaaaatgcc ggtgtactcc ttctggtgct caccgaatgt ctcataatcc
     5281 ataaaaagat ttacgatatc ctcacccgaa agagaatcta ccagccaggc cgcatattta
     5341 tctgcggtaa gaggccatcc atcccaactc ttattagaga atctgaaagc tatgtcatca
     5401 ctcagactgg agtttctgag caaaagtttc tgtcggggat taatggcatt ggtatagacg
     5461 aaatttgggc tcttccagcc aagaatatgt tttgcaccct cggttagcat tacattatat
     5521 cccagatcgg ctactgcatc tcctatggag tcggagtaga taagctcggt gttgcggaat
     5581 gcggtgggtt ttactccaaa tacccttgta atcatctctg catgctgttt aacctgcttt
     5641 ttaaactcag cgggtgatga tagagaggca aggctatgag aataggtctc cgcgaggaaa
     5701 tccacacatc ccgtagcagc aagggctttg aaactctcaa tcacctcagg tgcatacata
     5761 agaaactgct ctacagccac accggagatg gaaaatgtca ctttgaaatc tttgccgtgc
     5821 tttttaatta ggtcaagcag gatattattt gccgggaggt aacatttgtc tgccactctc
     5881 ctgagtatag tacggttagc aaactcgtcg aaataatgaa aatcgttgcc gatatcaaaa
     5941 aagcggaatt gcctgagtct gtccggctga tgtacctgaa agtataaaca aattgacttc
     6001 atatctgtaa tttttatttt ttcgcattta tcgcagcctc gtagaccctc ttgaccttgg
     6061 ctgctgcgtg gtcccacttc aaagagttaa cctcttcaag gccactatct atcgccattt
     6121 ttgaaagggc cggatagttg agcaagccat atatagcatc tgcaagggca tctatatccc
     6181 agaaatcaac cttgatggca tatcttaaca cctcggctac acctgactgt ttggaaatga
     6241 tggtaggcac atttgaaatc atagcctcaa ggggtgagat accgaagggt tcagagaccg
     6301 aaggcatcac atagacatcg gaatatgcaa acatacgctg cacatcctgt cctctcaaaa
     6361 agcctgtgag gtgaaatctg tctgatatgc ccaattgtgc cacacgttta atggaacgat
     6421 tcatcaagtc accgcttcct gccataacga agcgaacatt cggatacatt ttcagtacct
     6481 tattggcagc ctcaataaaa tattcgggac ctttctggaa ggtaatgcga ccaaggaagg
     6541 tgacaatttt ttctttcaca ccccgctgca cttcgatatc actgcggcct gaaaaatcca
     6601 ctgcattgtg tacggtgata accttggagg gatctatgcc atatttattg attacgatgt
     6661 tacgtgtcag attactcacg gttatgacct tatcagcttc gcgcatgcct gtctgctcca
     6721 gacggaatac ttcaggattg acgttttcgc ctgtacggtc aaactccgtg gcatgaacgt
     6781 ggattaccag cggcttcccg gaaaccttct ttgcagctat gcctgccaga ttggtaagcc
     6841 aatcatgcgc atggatgaca tcaaactgat gctgttgagc aatagcacct gcaaccattg
     6901 cataacgggc cacctcttcc ataaggtttg ccccatattt gccggaaaac ttatatttct
     6961 ccttaaaata acccttaaac tcttcggtct gatacttctg ttcctccgta accagttctc
     7021 taaatgagat cgggtcaatg taaggtctga gattggaacc cacgcggaga aaatccacct
     7081 tgcccaaaaa ctcgtcaata ttcttgcagg tatcataaac agcaacatcg ctggcattta
     7141 taattcttac tgcgctttgg tcctcatccc cggaagcaga aggcatcacg aaaagcactt
     7201 ccacattgtg gtgggcaagt cctttcgtca atccgtaaca ggcggtaccc aagccacctg
     7261 caatatgagg aggaaactcc catccgaaca tcagtactct catggctact cctccaattt
     7321 atatttgttt ataagatatt tgacacggat aatctcagcg gtagccgatg ccgatagtat
     7381 ggctccgtga ggatggtgcg gaggatctcc gtcatatatc tcagctacca ttcctattcc
     7441 gtggatattc atatcctcct caaaaccact taccagtgct tgtgaagtgc gtaggtaaga
     7501 agctccgtag agtttaagac aagaggcgat gtaggcgccg agaagccagg gccttgtgct
     7561 tccattgtgg gtagcaagat cacgctctat ctggttgccc tcatagaccc ctttatagtg
     7621 gggattctta ggtgaaagag tcctgactcc gcgtgtcgtt atgagttcct gattgataga
     7681 gcgcagaata tcatcctgta ccgtctcgct gaccggactg tagtcgaggg agcaagcata
     7741 tatctggtta ggtctcgtac aaatattctg tccaagacca tcaacgtagt ctgcaagatg
     7801 acgtctcgat tcaacccaga acagattgaa gaaattatct tctatttttt tcttaattgc
     7861 atcacactcg cgggtgaagt cattttcttt tgcaaaactc ttctccatcg cagaagcgaa
     7921 gcaaagcgcg ttataccaaa aacaattggt ctcaacctgg tatcctgcgc gctcggtcac
     7981 gggacggcct ccggcatagg catccatcca ggtgaggggt acaccggact tctgtgccca
     8041 taacagacca ttattgtgca ggatcacttc aggacgcccc tggataaagg agtgtatgat
     8101 gtccttgagg attgggccat attttgacca aaccttagcc gactctttgg tataagagat
     8161 gagatgctgc accaactccg taaggcgtaa aggagcctca acctggtcgc atccttttag
     8221 aatggcctca ttatgggtgg caatggtatc atcgagaatg cgcctgaaga gcttcacatc
     8281 gccgttgttg taaagggtca aaccgggaag cgctacgcaa gtctctctca acagacccga
     8341 ttcgagccag gagtatccgg cacaaatttc aattttgtcg tttcgggatg taaaaagctg
     8401 cattgctgca tccctgagac aatcttcata actctcctga ggctcacgca cattaagttc
     8461 tcttgtaaat ttagttttta ggttgttcgg gttagattca ctggttgagg cggaaaatat
     8521 tatcgtctca cctttattga taggcatttc gaagtatcct ggaacgaata gatcttcggt
     8581 acagtcaaat ccgcgacgat actcctctct gtaaacgatg ttgtagtacc aatccggaga
     8641 gggtacatag tcgcaaggtt tggaaatctg caagttgaga tcgggaaaac cctcgtacat
     8701 tttaaaagcc actccgtttt ctatactcct gtaacgggta tcggcatcac tattggccct
     8761 tgtcagagag tggatgttgc ggaatgcaag aaaaggcttg agcctaagga tagtgttgct
     8821 atgtgcatcc acaagggtat atttgatcag cagctggttt tcatttttag tagagaagat
     8881 gatgcttttg cggaacacca taccgccgac acggtatgtg ataacgggta tggggtccat
     8941 ttcaaagtca ataatatact tatgaccttt gggttcaaat acggagccat agttatgaat
     9001 accgaggtta aaaggtcgtc cgtgctgaat gagcgtctca tcgagagagg acaacaggat
     9061 atgcttgctc ccgccgaaat tgtcaacggg aactgccagc agagcgtgat attttctcgt
     9121 attgcaacac acgatggtcg tatttgtata gccaccggcc atattggtag aaatcaactc
     9181 gcgcttgaga gagtattcga gatttaccag ttccgatttg ttgaatttca agtaagccat
     9241 atagttatat tgtaattatc gtgttgtttg cacctatttt gaccttgttc tgaacccgca
     9301 aaattagata taaaaattca atatttcaat tttgtcacct aatcgtcata tctcaaaacg
     9361 agagcgctgc gacatggtat atagagtgat agtaggtttt gcccctcctt ataccgggta
     9421 aagtgctcta caccagcatc attcctgcca aaaccgccaa attccggagc atcgctgtcg
     9481 agtatgggta cccatttgcc ggcaggggcc tcaaaacagt aattttcgaa agaatgttcc
     9541 ggattgaaac tgaaaacgaa tagaaatgca cctctggtaa agatgagaat cttcttttcc
     9601 tcgtcctgac ggagagatac gatcttgtat ttcaatatat tgttgtcggc aaacagatgt
     9661 atcatagatt tgtcgaaatc acctaacgat ttgtaacgga gatagtctgc atcggccaat
     9721 gaccactgtc tgcgtgcata gaaatatgac caggaatttc cctccctggg aaaatcgatc
     9781 cactcaggat gcccgaattc attacccatg aagtttagat agccgtcacc ggcagttgca
     9841 agtgtaacca ggcgtatcat cttgtggagt gccatacccc tgtctacaat gatgttctcc
     9901 gactccttgt tcatggatga atacatctcc ttgtccataa gccggaatat tatcgtctta
     9961 tctcctacaa gagcctggtc gtgacattcc gcatagctga tggtcctctc gtcagcacgt
    10021 ttattggtaa gttggtaaaa gatctctccc atgctccaat cccaatccga caactctttg
    10081 atccacttga tccagtgatc tgcaacaccc atactcatcc tgaaatcgaa tccaaagcca
    10141 ccctttttta tcggagctgc cagacccggc atgccggaca tatcctcggc aatggtgaat
    10201 gccaatggtt ttacttcctt tacaagaata ttggcaagac ccagataaac cagcgcatcc
    10261 tcatcctgac cgccgtcaaa atagcaatca taccctatga aattgcgttc cagaccatga
    10321 tcatagtaaa gcatgctggt aactccgtca aaacggaatc catcgagatt gtactcctcc
    10381 aaccaatatt tacagttgga aagcaggaaa tagagtgtct cgttcttacc ataatcaaag
    10441 cagcgcgaat tccaggcggg atgctctccc ctctctcccg cgtggaaata ggtagtataa
    10501 gagccgtcga gacgactgag accctccacc tcgtttttaa ctgcgtgaga atggacaata
    10561 tccattatta cggcaatgcc gagccggtgc gcctcatcca ccagttcctt gaactcctcc
    10621 ggagtaccga atctgctgct aagggcaaaa aaattggaga cctgatagcc aaaagaaccg
    10681 taataggggt gctcctggag ggccattatc tgtatggtat tgtatcctcc ggctgcaatc
    10741 ctggggagga catcacgcct aaattcgcta aatcccgcca ctctctcctc ttcagagctc
    10801 ataccgatgt gggtctcata gatcaaagga tttttcaccc tgcgacggaa ttttctcttc
    10861 catttgtatg gtgcggatgg ggcccatacc tgggcagaaa agatttttgt gtgttcatcc
    10921 tggacacagc gagtggcata ggagggtagg cgctctccgc caccgccggg ccattcgaca
    10981 taccaacgat aaagggttcc atgggatatg atttccgcag gtactaccaa ctcccaatta
    11041 cctccgccgg taggtgtaaa agtatacctc tcctcccttt tccagccatt aaaatcacct
    11101 ataagccaga ttctggtggc attgggtgcc cattcacgga aaacccatcc ccggttatca
    11161 cgatgcagca cataataaag atggttgttg actacatcgg acaggcggcc ctctccggcc
    11221 atttgccgtt tcttttgaag caccatggca ttacggcgct ctataatctc cttgtagggt
    11281 tcaaggtatt tatctttttt atatattttc atcgctacca ttcctctcat tgataatcct
    11341 ttctatcttc tcctgctctc cacgtattgc acttcggcaa tattctatca gctccatagc
    11401 tctttttacg tcattttcaa tgccggagag gtccctctca ggatcttcta tgcctactac
    11461 gagccgttcc agctcggcaa aggcctcttc ataactcatt ttctctttca tatagcatta
    11521 ttctttattt cgcttgatac aacctcacat tcaaccaccg catcactgaa cattacttta
    11581 agtcgctctc ccggcacgag ttccgcaccg gtagcgatcc tgcgtccatc ttttagcact
    11641 acaagataac ccttttcaat cagagtacgc ggatttgcag ctatcagacg cgtctgcaaa
    11701 aggtctaccc tacgtaaagc ctccgaagct tttaaagaga cggcactcct gatgcgtact
    11761 ttcatattgt caaaaagagc cagttcacgg tggctcttgc tcttaagagc taatttaagt
    11821 cgggaaatca aggctgccac agcctgctct tcatgaacaa acatctctat gaaaaaatct
    11881 gccagagcgg taggagtctt aaggttgtgc caggcaacca tatctatgac atggtaatct
    11941 ttgtcatgcc ctatgccggt aagaaccggt agcggaaact gggctacatt tagcgcaagc
    12001 tcataatcat caaaacagac caaatccata accgaaccgc cacccctaag tatcaatact
    12061 gcatcaaatt catcaatgcg ttgagcgata gtatcgaggg cagcaataat gcctgaagga
    12121 gcctctgcac cctgcatcgg agcaggaaat agtgtggtgg caaaagtaaa cccatattga
    12181 ttgccgtgaa ggtgttccat gaaatcgcgg taacctgctg cagtttcagc cgaaatgacc
    12241 gccagactac ggggaagcgc aggtaactca agagaagagt taatatccat catcccctcc
    12301 tccgagagac gtgcaatcgt tctctgacgt tccaattcca aattgccaac cgtaaaggaa
    12361 gggtcgatat ctcgtatatt tagcgaaagg ccgtacagct cactataatt tacctgtacc
    12421 tttaccaaaa tgttcattcc ggctacgatg tcgctgccgg tgacgctacg aaagtgtgca
    12481 gataagatgc gataggtctg ggcccatatt actgcagaac attttgcgag caaccttcca
    12541 ctcttctctt ccttttccac taatgtcaaa tagcaatggc ctgagcgatt tacgctgcat
    12601 tcacttacct ccgatctgac ccatagagaa tcagtgaaag cctcggccag tacctcggag
    12661 atatcgctct gcaactggta cagcgtatag agttttccat catccataac atgacaaaga
    12721 taatgattat tgggtaaata tatttctata attcaagtta cttccgtaca tttgcaaata
    12781 agaattcaaa gtgatgatta tcaaaaaggc tatttttgca ggcagcagcg aaagagttgc
    12841 ccagaagccg aaagcactct atcccgagtt cgcttttatc ggcaggtcaa atgtcggcaa
    12901 atcatcactt ataaactcct tgtgtcagaa taaaaagctt gcccacacct cttcaactcc
    12961 gggcaagaca cagctgatca accacttttt gataaatgac agttggtatc tcgtagacct
    13021 tcccgggtac ggctacgcca aaataagcaa gaccgccaga gccaaattgg cgaaaatcat
    13081 agatggctac atagaccttt ctcaggaaat gatactgctt tttgtcttgc tggattccag
    13141 acatgacatc caacagatcg acctcgattt tttgatatct ctcggagaga gggccattcc
    13201 attttcaata attttcacca aatgcgacaa atctcctaag aatgttcttg agaaacagat
    13261 tgagaagaac cggaaccaac tgctcgaata ttgggaagaa cttcctccca tatttttaag
    13321 ctcttccgta accggttcag gccgtgaaga aattctttcg tatattgact ccatactctc
    13381 aaccctaaac tccgagcctt aacccgaaac acgaaaccca taacacacaa caaataacac
    13441 gcaacacgca acacgcaacc cgtaa
//
LOCUS       DAOSIE010000010        13417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_4689_length_13417_cov_4.245772, whole genome shotgun sequence.
ACCESSION   DAOSIE010000010 DAOSIE010000000
VERSION     DAOSIE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13417
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4689_length_13417_cov_4.245772"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLI69_00780"
     CDS             complement(<1..750)
                     /locus_tag="PLI69_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41058.1"
                     /translation="MKRKYFLTILTLFFGAIILFAQNANEPQYKSVLSRDSILIGDQI
                     EWRIPVKIYEGEDYYIERPDEPVAPGVETIKGFHIDTLSFRRGRLEVEGRMTLTAFDS
                     GSYFLPPVIVHIERADGSVDTLIYDGPILDVNTIQIDTATFVPFDIKGQIKYPVTFSE
                     ILLWGGIVLIVVALVWLIVRFVKRSGEGRSFFGRPIVKDPPHIVALRSLEKIRGQKLW
                     QNNRHKQFYTAVTDTLRKYISDRYHIAAMEQT"
     gene            complement(750..1631)
                     /locus_tag="PLI69_00785"
     CDS             complement(750..1631)
                     /locus_tag="PLI69_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOG41059.1"
                     /translation="METDLLKKVRKIEIKTRALSHQIFAGEYHSAFKGRGMAFSEVRE
                     YQYGDDVRSMDWNVTARLRTPYIKVFEEERELTVMLLIDVSGSRAFGTVGKSKKDLMA
                     EIAAVLSFSASINNDKVGALFFSSKVEKFIPPKKGRSHLLRIIREILEFKPEEKGTDI
                     GEALRYLTNAIKKRCTAFLLSDMLDTDENESPRYEEALKIAAGRHDLSVINIYDPRER
                     EIPDVGLIRIRDAETGQGIWVDSSSSKVRSSFAQWNRELYEDTIHLLRRYKVDNVSIS
                     TNQDYVKELISFFKNRA"
     gene            complement(1643..2635)
                     /locus_tag="PLI69_00790"
     CDS             complement(1643..2635)
                     /locus_tag="PLI69_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG41060.1"
                     /translation="MDSVNIKELNDRIERESAFVDIVTMEMNKVIVGQKHLVENLLIG
                     LLADGHILLEGVPGLAKTLAINTLASIVDAKFSRIQFTPDLLPADVIGTLIYSQKSEQ
                     FQIKKGPIFANFVLADEINRSPAKVQSALLEAMQERQVTIGDDSFPLPQPFLVMATQN
                     PIEQEGTYPLPEAQVDRFMLKVVVSYPKKEEEKLIIRMNNSGTFPKPSTVLKPEDIVR
                     AREVVRDVYMDEKIEKYIVDIVFATRYPEEYNLPKLKDMIAFGGSPRASISLSLAAKA
                     YAFIKRRGYVIPEDVRAVCYEVLRHRIGLTYEAEAENVTTEAIIAEILNAVEVP"
     gene            2704..3465
                     /gene="truA"
                     /locus_tag="PLI69_00795"
     CDS             2704..3465
                     /gene="truA"
                     /locus_tag="PLI69_00795"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG41061.1"
                     /translation="MRFFVSISFNGGAYSGWQIQKNAISVQAVMERALTLATGETISV
                     TGAGRTDTGVNAKGFIAHFDVSPECSKKPNEILRKINAILPSDIIAHNICQVEDDAHA
                     RFDAISRTYRYYVHTEKDPFAIFSYYYRHPLDVERMNVAAKHLLGTKDFSSFEKLGGD
                     NKTSICSVISAQWEEIDKTHLCFCVTADRFLRNMVRAMVGTLLDVGRGKREPEWIAQL
                     IEEKERCKAGQSVPGHALFLTEVRYPYPIFENNKK"
     gene            3549..4181
                     /locus_tag="PLI69_00800"
     CDS             3549..4181
                     /locus_tag="PLI69_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOG41062.1"
                     /translation="MSLIDMAIKGGWLMIVLALLSIIGLYIFGERWWAIRRAGKISKT
                     FMKDVNDYILDGKIKSALGLCKKTSSPIARLVEKGIERIDKPLTDIQAAVENVGNLEV
                     AKLEKSLPFLATIAGGAPMIGFLGTVMGMIQAFFNMSQAGNNIDISLLSDGIYTAMVT
                     TVGGLIVGIMAYFGYNYLTARVSDVVFKMENVTIDLLDTLQSKQEDSNLY"
     gene            4189..4602
                     /locus_tag="PLI69_00805"
     CDS             4189..4602
                     /locus_tag="PLI69_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOG41063.1"
                     /translation="MAIKRKTKPEASFSMSSMTDIVFLLLLFFLITSTLVNPNALKLL
                     LPKSTGQISAKATVSVSIKHYPEQGMVSYHINGNQIPVRFDQIEGSVVTLLQGEDDPT
                     FSIYADETVPIKEVVALMNIAKRNKFKVIMATSPE"
     gene            4613..5368
                     /locus_tag="PLI69_00810"
     CDS             4613..5368
                     /locus_tag="PLI69_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOG41064.1"
                     /translation="MNSGVRDGKSNMFGIGLTVIFHIALLFIGVGSGLKYNYPPPEEQ
                     AILMDFISEEEFKPIEVATGVEPRAPNPNPEEEVRLVQRSDGPVEGTKSNEALEATVG
                     DDGDVEVPEPPRKEINRRALFSVADNNKKDTLAPQTAETPSGNLTAGHAQGNTRVGST
                     DGTPSARLQGRSVMGSLPLPEYSVQASGRVVVRIMVDKNGNVTSAQPGVAGTTVTNRT
                     LWEAAKEAALKAKFNTSSTISQEGTITYIFTLK"
     gene            5424..6983
                     /locus_tag="PLI69_00815"
     CDS             5424..6983
                     /locus_tag="PLI69_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOG41065.1"
                     /translation="MKRLISTFIMLMVVISVSFSQELPKREFRGAWLHIVGNQQMRTM
                     TMEEIQEWFINSLDRLQQLGCNAILFQVRPQADAFYESDIEPWTRFLTGEQGKAPAPY
                     WDPMAFLIKETHARGMEFHAWLNPYRVTSNDTEVLHPDHLYFKKPEIFKRYGKQLYFD
                     PGEPESVSHTVKVIVDIVNRYDVDAIHFDDYFYPYPVPYEEFHDDASFIKYGKAQGFE
                     YWQKNDWRRNNVETLMREINSAIKAIKPWIRFGISPFGIHRNLKDTPDGSGSKTNGLS
                     NYEQLFADVPGWAEKGIIDYIVPQLYWKIGHPAADYDVLINWWNDSNFKGHLYIGQSI
                     STFNEPDLDNPQMTQMPRKMELVRTLPNVDGNVWWPGWTLTPAPVIKAEGERQTPPRR
                     AEQAPSLPFSITDSLENVYQRCPALIPAYTDLDAINPDPVESMKIRHGSIVWEVESTD
                     DVMQEPHFYVVYRFPENEEINLDDSQYIVKITKDQSFKPEGKRDRKFKYVVTVVDRCW
                     NESAASKAVVW"
     gene            complement(7016..8641)
                     /gene="pruA"
                     /locus_tag="PLI69_00820"
     CDS             complement(7016..8641)
                     /gene="pruA"
                     /locus_tag="PLI69_00820"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOG41066.1"
                     /translation="MNNAIFSFPLPANEPVKTYLKGSPEREALEAELKKQSSQVIEIP
                     LIIGGKEVRTGNIGKVVMPHDHQHVLASYHKAGEKEVQMAIDAAMAAHKEWSELDWTV
                     RATILLKVADLIAGKYRAVINASTMLGQSKNIYQSEIDAACELIDFLRYNAHFAGKLY
                     GFQPKSAPGQINSVEYRALEGFVFALSPFNFTSIACNLNMSPILMGNVTVWKPATTAI
                     LSNYYLMKVFKEAGVPDGVINFVPGQGSAIGNVVFNSKHLAGVHFTGSNATFNNLWKQ
                     AANNLEKYVSYPRLVGETGGKDFIFVHPSSCVKEVATAAFCGAFEFQGQKCSAASRMY
                     VPASLWPSILEAIKAQAASITMGDVCDPSNFVNAVIDEVSFDNIVSYIELARNSKDAK
                     IVMGGKYDKSKGYFVHPTVIETTNPHFKSMVEEIFGPVLTVYVYKDAEIDKAVELCDT
                     STIYGLTGAVFGRDRMAVQKIADRLRYTAGNFYINDKPTGAVVGMQPFGGSRASGTND
                     KAGGEFNLIRWTIPRTIKETLVSPTDYRYSYMK"
     gene            8727..9491
                     /locus_tag="PLI69_00825"
     CDS             8727..9491
                     /locus_tag="PLI69_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG41067.1"
                     /translation="MKTALVQHNIVWAEPEINCNNISRLLENFPQVDLIVLPEMFSTG
                     FATKPEGIAENEPSYSLEWMKKSAAKMDCAIAGSVALKTEGGYINRFYFVKPDGDVIT
                     YDKRHLFTYGGEHLTFKPGNQRVIIEWRGVRILPIICYDLRFPVWSRYRGDYDMIICV
                     ASWSIPRRYHWDTLIRARAIENQCYMAAVNRIGTDPSCEYDGGTVFIDPYGYPVAACS
                     DNKEEVIICETDLKLLKKYRKRFPALRDRDDFSILL"
     gene            complement(9527..10360)
                     /locus_tag="PLI69_00830"
     CDS             complement(9527..10360)
                     /locus_tag="PLI69_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG41068.1"
                     /translation="MGILLKLNDVKESLLIISIIAIVLLLVAIVLLFYNVKLYRSLSR
                     SEQDKSRIKRQLTQNAAHEIKTPIASIHGYLETLVANPDLPEEKRRHFLERCLSQSER
                     MTRLMAYMSTISRLDEGRVTMEEGVDLERIINDVIEDFKSKLDSAGISVKLAIPEYLF
                     VRGDQGLLYSIFRNLVENTVSYASGADTLRIACVRREDGFEFEVSDNGSGIADKHLNR
                     IFERFYRVDKGRTRGEGAAGGTGLGLSIVKNAVLAHGGTISASSGPSGTGLIVRFSLP
                     A"
     gene            complement(10335..11036)
                     /locus_tag="PLI69_00835"
     CDS             complement(10335..11036)
                     /locus_tag="PLI69_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG41069.1"
                     /translation="MCMARILVIEDEMDLCEILQFNLEQDGHKVDIANSAETAMKMDI
                     SSYDLLLLDVMLGGISGFRLASILRENPATKQVPIIFTTALGESSDVVRGLEVGGDDY
                     ISKPVRIAELKARIHAVLRRYEQQQGRAASVDNTIKHQDIELTIDAEQKAAFIDGHDL
                     KLTKLEFEVLNLLFSNSPKVFSREQIITAVWPSDVIVTDRTVDVNITRLRRKLGPLGS
                     CIKTRIGYGYSFETE"
     gene            11192..12898
                     /locus_tag="PLI69_00840"
     CDS             11192..12898
                     /locus_tag="PLI69_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOG41070.1"
                     /translation="MLLQILTLLGALGMFLFGMNMMSSGLQKAAGDRLRRLLSSITSN
                     RFKGVLTGLIITSIIQSSSATTVMVVGFVNAGLLTLLQAIGVIMGANIGTTMTAWIIS
                     LLGFKADISILAVPLMALGFILSMMKSEKRKNIGEFVIGFALLFIGLAFMKSSVPDLE
                     STPEVLSFIRGWGDYGFGSVLLFVLLGTVLTLILQSSSATVALTMIMLTLGWMPFEHA
                     AAMVLGENIGTTITANIAAAVGNTSAKRAALAHTVFNLFGVIWALILFRPFVGFIGSI
                     IAAMGLPNPAHMGTEVASGDNQTALLYGLSMLHTVFNIITTLILVWFCPQIAKLVSAI
                     IRMPKSTEDQEVFRLKYIEGGPVSTAELSIKQALKETVHFGRISQKGLDYALKAVNSK
                     TGDEFDTYRKKLVKYEEISDRMELEIAHYLHKIPTADISTGTSEQIRTLYRIISELER
                     LGDSGERIGNIIVRRNLHNKRFNEEEIKNISNMFDRVDNAYEVMISNLTRAADGKPVD
                     LIAAQNAEVQINQMRNDLRDEEMYKMEGKGDFLTSVYYLDLISELERMGDFIINISQA
                     LA"
     gene            13097..>13417
                     /locus_tag="PLI69_00845"
     CDS             13097..>13417
                     /locus_tag="PLI69_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOG41071.1"
                     /translation="MLLQILTLLGALGMFLFGMNMMSSGLQKASGDKLRKLLSSITSN
                     RFKGVMTGLSITAVIQSSSATTVMVVGFVNAGLLTLLQAVGVIMGANIGTTMTAWIIS
                     LLGFK"
BASE COUNT         3727 a         3153 c         2939 g         3598 t
ORIGIN      
        1 ggtctgctcc atcgcggcaa tgtgatatct gtcactgata tatttgcgca gagtatcagt
       61 caccgctgta tagaactgct tatggcgatt attttgccaa agtttctgac cgcgtatctt
      121 ctcaagtgag cgtaatgcta ctatatgagg gggatcctta actatcggac gaccgaagaa
      181 ggaacgtccc tccccggatc ttttcacaaa acgtactata agccacacga gagcaaccac
      241 tatcagcaca atacctcccc aaagaagtat ctcggagaag gtcaccggat attttatctg
      301 tccctttata tcgaaaggaa cgaaagtggc agtatctatc tgtatggtat tgacatcgag
      361 aataggtccg tcatagataa gagtatcgac ggagccgtca gccctctcta tgtgtacaat
      421 caccggcggc aggaaataac ttccgctatc gaatgccgta agtgtcattc tcccctccac
      481 ttcaagtctt cctcgcctga aagaaagggt atctatgtgg aatcccttaa tggtctcaac
      541 tccgggagct accggctcat ccggtctttc tatgtaatag tcctcccctt cgtagatctt
      601 taccggaata cgccactcga tctggtctcc tatcaatatg gaatccctgc tgagcaccga
      661 tttatattga ggctcattag cattctgggc aaatagtata atcgcaccga aaaagagtgt
      721 caatatggtc aaaaaatatt ttcttttcat caagctctgt ttttgaaaaa cgaaatcagc
      781 tccttgacat aatcctggtt ggtgctgatg ctgacattgt ctaccttata cctacgcaaa
      841 agatggattg tatcttcgta aagctcccta ttccattgcg caaaactgct gcggaccttg
      901 gaggaagagg agtctaccca gattccctga ccggtctctg catcacgtat tcttatcagg
      961 cctacatcgg gaatctccct ttcacgagga tcatatatat tgattaccga gagatcatga
     1021 cgtcccgccg caatcttaag agcctcttca taacgcggag attcattctc atcagtatcc
     1081 agcatatctg agagcagaaa tgccgtacaa cgctttttta tggcgttagt aagataacgc
     1141 agcgcctcgc ctatgtcggt acccttctcc tccggtttga attcgagaat ctccctgata
     1201 atcctcaaca agtggctacg ccccttcttt ggaggaatga acttttccac cttgctgctg
     1261 aaaaagagag cccctacctt atcgttgttg attgatgcag aaaaagagag tactgcagct
     1321 atctcggcca tcagatcctt cttggattta cctaccgtac cgaaggccct tgagccgctc
     1381 acatctatca gaagcataac ggtaagctcc ctctcctctt cgaacacctt aatataggga
     1441 gttctgagac gagcggtgac attccagtcc atactgcgta catcgtcgcc gtattggtac
     1501 tcgcgtacct cgctgaaagc cataccacgc cccttgaagg cggaatgata ttcacctgca
     1561 aagatctgat gagacaaagc gcgggtctta atctcaattt tcctgacttt tttcaacaaa
     1621 tccgtttcca tccaattatc tattatggaa cttcaactgc atttaaaatt tcagcaataa
     1681 tggcctctgt agtgacattt tctgcctcag cctcataggt caggccaatt ctatgtctga
     1741 gcacctcata gcatacagcc ctgacatctt ccggaataac atagcccctg cgcttgataa
     1801 aggcataggc cttggcggcc aatgatagcg aaatactggc tcggggggaa ccgccgaatg
     1861 caatcatatc cttgagtttg ggcagattgt attcttcagg ataacgggtg gcaaaaacta
     1921 tgtcaactat atatttttca attttttcat ccatatagac atccctgaca acctcgcggg
     1981 cgcgcacaat atcctcgggt ttaagtaccg tggagggttt ggggaaagtc cccgaattgt
     2041 tcatgcggat aatgagcttt tcctcctcct ttttgggata actcacaact actttaagca
     2101 taaatctgtc tacctgtgcc tcgggcagag ggtaggtgcc ttcctgctcg atagggtttt
     2161 gggtcgccat cacgaggaag ggctggggca gagggaaact atcgtcaccg atggtcacct
     2221 gacgctcctg cattgcctcg agtaatgccg actgtacctt tgcaggggaa cggtttatct
     2281 catccgcaag cacgaagttt gcgaagatgg gccctttctt aatctggaac tgttcgctct
     2341 tttgactgta aataagcgta ccgatgacgt ctgccggcaa aaggtccggg gtaaactgta
     2401 ttctgctgaa ttttgcatcc acaatggatg cgagagtatt gattgccaag gtcttggcaa
     2461 gacccggcac gccttcgagg aggatatgcc catcggcaag aagcccgata agaagattct
     2521 ccaccagatg tttctgcccc acaatgacct tattcatctc cattgtaaca atatcgacga
     2581 aggcactttc gcgttctatg cggtcgttga gttctttgat gtttacactg tccatatatt
     2641 tataaattga atatttttgt acttttgtaa tttataacct acaaaattaa tatttttttt
     2701 ccaatgcggt tctttgtctc aatctctttc aacggcggtg cttatagcgg atggcagatc
     2761 cagaagaatg ctatctcagt gcaggccgtc atggagaggg cacttactct tgcaaccgga
     2821 gagacaattt ccgttaccgg cgccggaaga accgataccg gggtcaatgc caaaggattc
     2881 atagcccatt tcgacgtttc tcctgaatgt tccaagaagc caaatgagat attacgcaaa
     2941 ataaatgcca ttctgccttc tgacataatt gctcacaata tttgtcaggt tgaagatgat
     3001 gcccatgccc gttttgatgc cattagccgc acatacagat actatgtaca caccgaaaag
     3061 gacccttttg ccattttttc atattattat agacatcccc tggatgttga aagaatgaat
     3121 gttgccgcta agcatctgct aggtacaaag gatttctcaa gttttgaaaa gctcggcggt
     3181 gacaataaga catctatatg tagtgtcatt tcggctcagt gggaagagat tgataagact
     3241 cacctctgct tctgcgttac tgcggatcga tttttgagaa atatggtcag agcgatggtt
     3301 ggcacacttt tggatgtcgg cagaggcaaa agagaaccgg aatggatagc acaattgata
     3361 gaggaaaaag agcgctgcaa ggcaggacaa tcagttcccg gccatgcact ttttctgacg
     3421 gaagtcagat atccctatcc catttttgaa aataataaaa aataaaaaca gatacattta
     3481 tgctttttac atttctacag acaagcactg aagtgattga gggcacaatt gccccggtgc
     3541 aagagaacat gtcattgatt gatatggcaa tcaaaggtgg atggctgatg atagtactcg
     3601 cactactctc catcataggc ctatatattt tcggtgaaag atggtgggcc atcagaagag
     3661 ccggaaaaat cagcaaaacc tttatgaaag atgtaaacga ctacatcctg gacggtaaaa
     3721 tcaaatcagc tttaggtctt tgcaaaaaaa cctcatctcc tattgccaga ctggttgaga
     3781 aagggatcga aaggattgac aaacccctta cagacataca ggcagcggtg gagaatgttg
     3841 gcaacctcga agttgccaaa ctcgaaaaaa gccttccctt cctggcaact atagcgggtg
     3901 gcgcacctat gatcggattt ctcggtaccg taatgggtat gattcaggca ttcttcaata
     3961 tgtcacaggc aggtaataac attgacatct cccttctctc ggatggtatt tatacggcta
     4021 tggttactac cgtaggtggt ctgattgtgg gcattatggc ctatttcgga tataactacc
     4081 tgactgcaag ggtatccgat gtagtattca agatggaaaa cgtcaccatc gatcttcttg
     4141 atacattaca gtccaaacaa gaggactcca acctttattg attaggatat ggccataaaa
     4201 cgtaaaacca agccggaagc ctctttttca atgtcctcta tgacggacat cgtgtttctg
     4261 ttgctcttat ttttcttaat aacctccaca ctggtcaatc ccaacgcgct caagcttctt
     4321 ttgcccaaga gtacaggtca gatatctgcc aaagctacgg tgagtgtctc cataaagcac
     4381 taccccgagc aaggcatggt ttcttaccat atcaacggca accagatacc tgtccgcttt
     4441 gatcagatcg agggaagtgt ggtaactctc cttcaggggg aagatgaccc taccttctcc
     4501 atctatgccg acgagaccgt tcccataaaa gaggtagtag cactgatgaa tattgcgaag
     4561 cgaaataaat tcaaagtgat aatggcgacc tcgccggaat aataaactga aaatgaactc
     4621 cggagtcaga gatggtaaat ccaatatgtt cggtatcgga ctgacggtga tatttcatat
     4681 cgcccttctc tttattggtg tcggctccgg actcaaatac aactatccac cccccgaaga
     4741 gcaggcaatc ctaatggatt ttatctccga agaggagttc aaacccatcg aagtggcaac
     4801 cggtgtggag cccagagctc caaaccccaa tcctgaagag gaagttaggc ttgtacagcg
     4861 ctccgatggt ccggtagaag gtaccaagag caacgaggcg ttagaggcga ccgtaggtga
     4921 tgatggtgat gttgaggtac ccgaaccacc ccgcaaggag atcaaccgca gggctctatt
     4981 ctcagttgct gacaacaata aaaaagatac tcttgcaccc caaactgcag agactccttc
     5041 aggcaatctc actgcaggtc atgcgcaggg taacaccaga gtcggctcta ccgacggaac
     5101 tccttcggca cgcctgcagg gacgctccgt tatgggttcg ctacctcttc ccgaatattc
     5161 cgtgcaggct tccggtagag tagtggtgag aataatggtg gataaaaatg gcaatgtgac
     5221 ctctgcacaa cccggtgttg ctggtactac agttacaaac agaacccttt gggaggcggc
     5281 aaaggaagcc gcacttaagg ccaaattcaa caccagcagc actatctcac aggaaggtac
     5341 cattacctat attttcacac tgaaataact ctcttttttc attatatttg ccgctgattc
     5401 atcaattaac agcaactact gtaatgaaaa gactcatttc cacatttatc atgctgatgg
     5461 ttgtcatcag cgtttctttt tcccaagaat tgccaaaacg tgagttccgc ggcgcctggt
     5521 tacacattgt cggtaaccaa cagatgcgga caatgaccat ggaggagatc caagagtggt
     5581 ttattaattc actggataga cttcagcaat tgggctgcaa tgccattctt ttccaggtaa
     5641 gacctcaggc agatgctttc tatgagtcgg atattgagcc ctggacccgt tttctgaccg
     5701 gagagcaggg caaagccccg gccccctact gggaccctat ggcatttttg atcaaagaga
     5761 ctcatgcgcg cggaatggag tttcacgcct ggctcaatcc atatcgcgtc acttccaacg
     5821 atacagaggt actccatccc gaccatctct atttcaagaa acctgagatt ttcaaaagat
     5881 atgggaaaca actctatttt gatccgggcg agcctgaatc cgtttcccat accgtaaagg
     5941 tcatagtgga tatcgtcaat agatatgacg tggatgcaat tcattttgac gactattttt
     6001 atccctatcc ggtaccctac gaagagttcc acgatgacgc atctttcata aaatatggaa
     6061 aagcccaggg gtttgagtat tggcaaaaga atgactggag gaggaacaac gtggagaccc
     6121 tgatgcggga aataaacagt gccatcaaag ctatcaaacc ctggatcagg tttggtattt
     6181 cacctttcgg aatccataga aatctaaagg acacccccga tggcagcggc agcaagacaa
     6241 acggcctctc caactatgag caacttttcg cggatgttcc gggttgggct gaaaaaggca
     6301 tcatcgacta tattgtaccc caactttatt ggaaaatcgg tcatcctgcc gcagattacg
     6361 atgtgcttat aaattggtgg aacgatagca atttcaaagg acatctctat ataggacaga
     6421 gcatctcaac attcaatgag ccagacctcg acaatccgca aatgactcag atgcccagaa
     6481 aaatggaact cgtacgcacc ctgcccaatg tggatggcaa tgtatggtgg ccgggctgga
     6541 cattaacacc cgcacctgtg atcaaagcag agggagaacg ccagacgcct ccccgcaggg
     6601 cggaacaggc gccctctctc ccattctcca ttacagactc tttggaaaat gtatatcaga
     6661 gatgcccagc tctgataccg gcatacaccg acctcgatgc catcaatccc gaccccgtag
     6721 agtctatgaa gatcagacat ggctctatcg tatgggaggt ggaatccacc gatgacgtga
     6781 tgcaggaacc acatttttat gtagtttaca gattcccgga aaatgaagag atcaatttgg
     6841 atgacagcca atacattgtt aaaataacaa aagatcagag cttcaaaccc gaaggaaaac
     6901 gagatcgtaa attcaaatat gtcgtaaccg tggtggacag gtgctggaac gaaagcgccg
     6961 catcaaaagc ggttgtttgg taactcaaaa aaaggcctct ttcgaggcca tttttttatt
     7021 tcatataaga gtacctgtaa tccgtagggg aaaccagagt ctcttttatt gtacggggga
     7081 ttgtccagcg aatgaggttg aactctccgc ccgccttgtc gttggtacct gaggcgcggg
     7141 agccgccaaa tggctgcatt cctacaaccg ctccggtagg cttgtcgttg atgtagaagt
     7201 tacctgccgt atagcgcagt ctgtcggcta tcttctgaac tgccatcctg tctcgtccaa
     7261 acactgctcc ggtaaggccg taaatagtag aagtatcgca aagctccact gccttatcaa
     7321 tctctgcatc tttgtaaaca tatactgtaa gcacggggcc gaagatctcc tcaaccatag
     7381 atttgaaatg aggatttgta gtctcaatga cggtaggatg aacaaaataa cccttgctct
     7441 tgtcgtattt gccacccatt actatcttgg catctttgct attgcgtgca agctctatat
     7501 aagaaacaat attgtcaaaa gagacttcat caattactgc atttacaaag ttcgagggat
     7561 cgcaaacatc tcccatagtt atggatgctg cctgtgcctt aatagcctcg agtattgaag
     7621 gccaaagtga agcgggaaca tacattcttg aggctgccga gcacttctga ccctggaact
     7681 caaatgctcc acagaaagct gcggtggcta cctctttcac acatgacgag gggtgtacaa
     7741 aaataaagtc cttgccgccg gtttctccga ccaaacgggg atatgagaca tatttttcaa
     7801 gattattggc tgcctgcttc catagattgt tgaaagtagc gtttgatcct gtaaagtgta
     7861 cccctgccaa atgtttggag ttaaagacta cattaccgat agccgatccc tgacctggaa
     7921 caaagttgat tacaccgtca ggtactcctg cctccttgaa gaccttcatc agatagtagt
     7981 tggatagaat cgcagtagtc gccggcttcc atacggtcac attgcccatc agtatggggg
     8041 acatattgag gttacaggcg atagaggtaa agttgaaggg acttaatgca aacacaaaac
     8101 cctcaagggc tctatactcg acgctgttga tctgcccggg agcgctcttt ggctggaatc
     8161 catagagctt accggcgaaa tgtgcgttgt agcgaaggaa gtcaatcagt tcacaggctg
     8221 catcgatttc cgactgatag atatttttgc tctgtccgag cattgtggaa gcattgataa
     8281 cagcgcgata tttacccgca atcaggtctg ccactttcag cagaatagta gcacgaacag
     8341 tccagtcgag ctccgaccac tctttgtgag cagccatcgc tgcatctatt gccatctgta
     8401 cctccttctc tcctgccttg tgatagctgg cgagcacatg ctgatgatcg tgtggcatca
     8461 ccactttacc tatgttgccg gtacgcacct ctttgcctcc tatgataaga gggatctcta
     8521 tcacttgcga actctgtttc ttgagttctg cctccagagc ttccctttca ggggaacctt
     8581 tgagatatgt ttttaccggt tcatttgccg gcagcggaaa agaaaatatt gcattgttca
     8641 taatgattat agttttaatt attccgctaa gatagcaaaa tcagaataat ttttctaatt
     8701 ttgatacatg ttattaacgg attgatatga aaacagcact tgtacaacat aatatagtat
     8761 gggcagaacc cgagataaat tgcaacaata tatcccgttt acttgagaat tttccacagg
     8821 tagaccttat tgtattaccg gagatgttct ccacaggttt tgccacaaag cctgaaggaa
     8881 ttgccgagaa tgagccaagt tactctctgg aatggatgaa gaagagtgct gccaaaatgg
     8941 attgtgccat agccggaagt gtagctctaa aaacagaggg aggatatatc aaccgcttct
     9001 attttgtaaa acctgacggt gatgtgataa cttatgacaa acgtcatctt ttcacatacg
     9061 gaggagaaca tcttaccttc aaaccaggaa atcaacgggt cataatcgag tggcgcgggg
     9121 taaggatact gcctattatt tgctatgacc tacgcttccc tgtttggagt cgctatcgcg
     9181 gggactatga tatgattatt tgcgtggcca gttggtcgat accccgccgt tatcattggg
     9241 acacactcat cagagccagg gccatcgaaa atcagtgcta tatggcagca gtaaaccgta
     9301 tcggcaccga tccgagctgc gagtatgatg gtggcacggt gttcatcgac ccttacggat
     9361 atcctgtagc agcctgcagt gataacaagg aagaggtaat catctgcgaa accgacctta
     9421 agctgctaaa aaaatataga aaaagattcc ctgctctcag ggacagggat gatttttcaa
     9481 ttcttttata gagtagtcag aaaaaccttc ggtaggtagt aaatttctat gccggcagcg
     9541 agaacctgac tataaggccg gtacctgagg ggccggagga tgcggaaatg gtacccccgt
     9601 gagctaaaac ggcatttttg acaatggaaa ggcccagacc tgttccacct gccgctcctt
     9661 ctccgcgtgt acgtcctttg tcgacacgat aaaacctttc aaaaatacgg ttcagatgct
     9721 tatcggcaat accggagcca ttatctgaaa cttcaaactc aaaaccatcc tctcttctta
     9781 cacacgcgat cctcaaagtg tcagctcctg atgcgtaaga taccgtgttt tctaccaaat
     9841 ttcggaaaat cgagtagaga agtccctggt cacctctaac aaaaagatat tcaggaatag
     9901 ctagcttaac cgatatgcct gctgagtcga gtttagattt gaaatcctct atcacgtcat
     9961 ttatgatacg ttccaggtct actccttcct ccattgtaac tctaccttca tcaaggcggg
    10021 agatggtgga catataggcc ataagacgtg tcattcgctc actttgtgaa aggcaccgtt
    10081 caagaaagtg acgacgcttt tcctccggaa ggtcagggtt ggccaccaaa gtctccaaat
    10141 agccgtgtat actggcgatc ggtgttttga tttcatgcgc agcattctga gtaagctgtc
    10201 gcttgatacg actcttatcc tgctcgctgc gcgaaagact cctatatagt ttaacattat
    10261 agaacagcag tacaattgct actagcagca gtactatagc aattatactg attataagca
    10321 gactctcttt cacatcattc agtttcaaaa gaatacccat accctatacg ggtctttatg
    10381 cagctgccca gaggacccag ctttcgacgc aagcgggtta tatttacatc aaccgtacgg
    10441 tctgtcacta tcacatccga aggccagaca gccgtgatga tctgctccct ggaaaagacc
    10501 ttaggagaat tgctgaagag caaatttaga acttcgaact ctaattttgt gagtttcagg
    10561 tcgtgtccat ctataaaggc cgccttttgc tccgcatcta tggtcaactc aatatcctgg
    10621 tgctttatag tattatctac cgaagccgct cttccttgtt gttgttcata ccgtctaagc
    10681 acagcatgta tgcgagcttt gagttctgct atcctgaccg gtttgctaat gtaatcgtca
    10741 ccaccgactt ccagacctct gaccacatcg gaactctctc ccagtgcggt ggtaaatata
    10801 ataggaactt gcttagtcgc aggattttca cgcaggatag atgcaagacg gaagccggag
    10861 ataccaccca acattacatc gagcagcaaa aggtcatacg aggaaatatc cattttcatt
    10921 gcagtttcag ccgaattggc gatatcaact ttatgcccat cctgctccag gttgaattgc
    10981 aggatttcgc acaagtccat ttcatcttca attaccagaa tacgtgccat acacaaggat
    11041 attttgtgca aatttacaaa tatctgtgcc attacaactc tattttttgg atttacgaca
    11101 aatgtcacac aattgtaaca caacttaaac cttagtgtaa aatattattg ccacttttgc
    11161 agcctattat acccttttta aaagtaatat tatgctatta cagatcctta cacttttagg
    11221 agccctcgga atgttcctgt tcggtatgaa catgatgagt tccggactcc aaaaagcagc
    11281 aggagacaga ctccgcaggc ttctctcttc aattacatca aatcgcttca aaggagtttt
    11341 aaccggcctg atcatcacat ccataataca atcttccagt gccaccacgg taatggtcgt
    11401 tggatttgta aatgccggac tgctgacact cttacaggca atcggagtta taatgggtgc
    11461 caatatcggt accactatga cagcatggat catttcattg ctgggtttca aggcggacat
    11521 atctatactt gcagtacctc tgatggcttt gggattcatt ctcagcatga tgaagtccga
    11581 aaaaagaaaa aatataggtg agtttgtaat aggattcgca ctactcttca tcggtctagc
    11641 gtttatgaag tcctcggtac ccgacctgga atcaacaccg gaagtgcttt cgttcatccg
    11701 gggatggggc gactacggat ttggctcagt attgcttttt gttcttcttg gcacagttct
    11761 tacactgata ctccaatcct ccagcgctac agttgccttg acaatgatta tgctcacttt
    11821 gggctggatg cctttcgagc atgctgcagc aatggtatta ggagagaata taggtactac
    11881 catcacagct aacattgcag ctgcagtcgg gaatacatcc gcaaagagag cagctctggc
    11941 ccatacggta ttcaatctct ttggggttat atgggctctg atacttttcc gaccattcgt
    12001 gggattcata ggaagtatca tagctgcaat ggggcttccc aatcctgctc atatgggaac
    12061 tgaagtagca tccggagaca accaaactgc tcttctatac ggcttgtcaa tgctccacac
    12121 ggtattcaac ataatcacca ctctcattct cgtatggttt tgtcctcaga ttgcaaaact
    12181 ggtgagcgcc atcatcagga tgcccaagag tactgaagat caagaggtct tccgcctaaa
    12241 atacattgag gggggtcctg tttccacagc cgaactctct atcaaacagg cactcaagga
    12301 gactgtacac tttgggcgca tttcacagaa aggtctcgac tatgctttga aggctgtgaa
    12361 cagcaagacc ggcgatgaat ttgacaccta ccgcaaaaaa ctggtaaaat acgaagaaat
    12421 atcagacagg atggaactcg aaatagccca ctatcttcac aaaatcccta ccgccgatat
    12481 aagcaccggt acttcagaac aaatccgtac actctacagg attatcagcg aacttgagag
    12541 actgggtgac agtggtgaga gaatcggcaa cattatcgtc cgtcgtaatc tccacaataa
    12601 gagattcaac gaggaagaga tcaagaacat ctcaaatatg tttgacagag tcgacaatgc
    12661 ttacgaagtt atgatctcaa atctgacccg ggctgctgat ggtaaacctg tggatctcat
    12721 tgctgctcaa aatgcagagg tgcaaatcaa tcagatgcga aacgatctac gcgatgagga
    12781 gatgtacaag atggagggca agggcgactt tctaaccagt gtttattatc ttgaccttat
    12841 ctcagaactt gagagaatgg gagacttcat cataaacatt tcccaggcgc tagcatagaa
    12901 tattgcttac gcgatagaag aaggtcgtgg aatatacatt ccccgacctt ctttttattt
    12961 tttgcttttc ttttacaatg gttttaataa aaaattgtgt acaaatagcc aaaattgtaa
    13021 tataattgta acatcagttg aagggtgtta caaaatatta ttagcacttt tgcacccgaa
    13081 aacatagtaa acaattatgc tgttacagat tctcacactt ttaggagccc ttggaatgtt
    13141 cctgttcggt atgaacatga tgagttccgg tctccaaaag gcatcaggag ataaactacg
    13201 taaactccta tcgtcgatta catccaaccg cttcaagggc gttatgaccg gtctgagtat
    13261 cacagccgtg atacaatctt caagtgctac tacggtaatg gtagtaggat ttgtcaacgc
    13321 cggacttctg acgcttttac aggctgtcgg agtaataatg ggtgccaata tcggcactac
    13381 gatgacagca tggattattt cgctacttgg attcaag
//
LOCUS       DAOSIE010000011        12721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_4987_length_12721_cov_4.381810, whole genome shotgun sequence.
ACCESSION   DAOSIE010000011 DAOSIE010000000
VERSION     DAOSIE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12721
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4987_length_12721_cov_4.381810"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PLI69_00850"
     CDS             complement(<1..604)
                     /locus_tag="PLI69_00850"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOG41072.1"
                     /translation="MIFLLDSHCDSPLMLVKGADFGQTVQQGYTPGKFPVTHVDFAKM
                     KKGGVNGSFFAIYTSNSLSPDESTRRAFELISSVYDAVEQNPDKVAFATTPAQAKRNK
                     KNGLISIFLGMENGAPIQKDLSMLRMFYRMGVRYLTLTHAGNNEICDSCAPKEKRWGG
                     VSPFGKEVIEECNRLGILVDVSHISDDSFWDVLKYSKAPVV"
     gene            726..1232
                     /locus_tag="PLI69_00855"
     CDS             726..1232
                     /locus_tag="PLI69_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOG41073.1"
                     /translation="MTQKEIKKFYDTHYRRLFNASYRIVGNGADAEEIMHDTLLRFLE
                     MENPPKEPAQISAWLIRTCIRRSIDRLRVIKREMSFKENYEEPESISAEESEEMEIST
                     REEVKRVKEALQTMKDPYRLVLTLSLVEGMDYQEIAEITGQKEVTIRSQVSRGKAALL
                     KILQNERN"
     gene            1219..1746
                     /locus_tag="PLI69_00860"
     CDS             1219..1746
                     /locus_tag="PLI69_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41074.1"
                     /translation="MREIDNIRKIFDDMEPAEGHFERFLKKANPSKRIEFRRNTPRKR
                     VFWKAVLFPVAASVMMIFGANLLIKTMNVKSDSFSKTVAVMEDPQDIYNAYMDVVRDC
                     YDYFSSLTSYDPRNGVSEHFNTLECITSEHIPLLEVLPEELSDREKAEILKEYYNRRV
                     ASVVELRKYVASIVR"
     gene            1800..2684
                     /locus_tag="PLI69_00865"
     CDS             1800..2684
                     /locus_tag="PLI69_00865"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOG41075.1"
                     /translation="MKRFILTFAAILGFVLTSGAQTVKSFDFKDFEALDISHWFDVEV
                     VRGNTYSIVVTISEDIEKYLLVKNSKGTLIIGLDNPSKRVLRNEDKIAKATITMPVLN
                     KLTLSGASKFNTSDRFDSGKNRFVMKLSGASLVYGLDVTAKDAQITISGASTVRELKG
                     DFANADIKVSGASKIYAKINADELDVEISGASKGEVEGEFEEVDLECSGASKAILSGM
                     ADKAIIEGSGVCSIVADNMSAKDVKIDLSGAAKAHVYVTRRLDVECSGASSCTYKTDK
                     SISITSEISRGSSLRRVD"
     gene            complement(2681..3430)
                     /gene="rlmB"
                     /locus_tag="PLI69_00870"
     CDS             complement(2681..3430)
                     /gene="rlmB"
                     /locus_tag="PLI69_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOG41076.1"
                     /translation="MEEKKQHYLYGMHPVYEAIRTGRKFEKILFKQGLEGDRFRELFD
                     LVKQNEIPFQFVPGEKMNKLCRGSHQGVTGYMSQIDYLPYEVIVEKALAESPNPIFLM
                     LDGVSDVRNFGAIVRSAECAGVSGVILPAKGGAAINADAIKTSAGALLRVPVSKVTNL
                     RIPLYYLIDAGFQVVCATEKAGGSIYDVDFTQPTVIVMGSEGKGISDSVLSLCNVEAA
                     IPMSGKIGSLNVSVATAIVLFEAVRQRMPQV"
     gene            complement(3435..5360)
                     /locus_tag="PLI69_00875"
     CDS             complement(3435..5360)
                     /locus_tag="PLI69_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOG41077.1"
                     /translation="MISISDLTVSFGGFTLLDSINFHISEKEKVGLVGKNGAGKSTIL
                     KLIMGLQSPTSGKVSMPADRKVGYLPQIMKHSRDKSVIEEAMTAFAEHNELNMRMDRI
                     SHELASRTDYESDEYHRLIIELNEISDQLRLYPLESPHVQAEKTLLGLGFRKGELDRM
                     TNTFSHGWNMRIELAKILLRNPDILLLDEPTNHLDIESIQWLEEYLKTFPGALVLISH
                     DRRFLDNCTNRTVEVMLGKIHDYKVPYSQFVELRKERIAQQRAAYDNQQRLIEKSEEF
                     IEKFRYKPTKSNQVQSRIKQLEKLERIEIDEEDLNTITVRFPSAPRSGDVVFSLKDAT
                     IGYSKPRKKIVLRGANMTIRRGEKVAFVGRNGEGKTTLMRLLTGQLQPFEGEALVGYN
                     VKTGYFAQNQEDLLDKKDTVYGTLDKIAVGDIRTKLRDILGAFLFRGDDIDKKVAVLS
                     GGERSRLAMAKLILHPYNLLALDEPTNHMDLRSKDVLKQALQKYDGTLIIVSHDRDFL
                     DGLVEKIYEFADGGVKEHLGGVDDFLYRKKISSLREIEIKQQSSAQPAPESKKEKFST
                     YEEQKYLSREERKIKNRRQRLENEISSLEEEIAQVESILASPTPEDDMVELTHKYQAL
                     KSDLETKMDEWASLTID"
     gene            complement(5357..6178)
                     /gene="tatC"
                     /locus_tag="PLI69_00880"
     CDS             complement(5357..6178)
                     /gene="tatC"
                     /locus_tag="PLI69_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOG41078.1"
                     /translation="MGNSNMSFWEHLDELRKVLFRSVAVLVVLMIVAFFFKETIFGNI
                     IFAPLTSDFCLYRWLDALLVLMGSEPIGEFSIELINIEMAAQFFTHVRISLYVALIVA
                     MPFIFYQIWTFVRPALYENEKRVIRRAFGFAGILFYIGIAVGYLLVFPLTVRFLGTYQ
                     VSADVPNHISLNSYIGMFVSLILIMGIVFEMPALAAVLSKLGIITKEFLKKYRKHALV
                     ILLILAALITPSGDAVTMLFVAIPLYFLYEVSILVCRSGKSETDEIAADAPGGEE"
     gene            complement(6200..6415)
                     /locus_tag="PLI69_00885"
     CDS             complement(6200..6415)
                     /locus_tag="PLI69_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOG41079.1"
                     /translation="MRNLLLFGSFGWQEILIIAFIVLLFWGGKKIPELMKGLGKGVRS
                     FKEGMKEVENEVKGIDDEPKNGDKEKK"
     gene            complement(6445..9108)
                     /locus_tag="PLI69_00890"
     CDS             complement(6445..9108)
                     /locus_tag="PLI69_00890"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOG41080.1"
                     /translation="MELPNKYDPSLTEDKWYSYWLENGFFHSEPDEREPYTIVIPPPN
                     VTGVLHMGHMLNNTLQDVLVRRARMTGYNACWVPGTDHASIATEAKVVEKLKAEGIEK
                     SDLTRDQFLKHAWEWRDKHGGIILEQLKKLGASCDWNRTRFTMDPDLSAAVINTFVHF
                     YKKGLIYRGVRMVNWDPEGLTAVSDEEVIHRETDSKFYYLRYKIAGGVSQGECDYVTI
                     ATTRPETIMADVAVCVNPKDERYLHLRGKNVIIPLINKEIPIIEDEYVTMDLGTGALK
                     ITPAHDINDYEIGLRHKLPVIDIINDDGTLNEQAQILVGEERFVARKKIVKMLEEAAL
                     LEKVEDYKSQVGYSERTNAVIEPKLSMQWFLKMEDLAQEALQVVEDDSVRLIPDKFKN
                     TYRHWMENVRDWCISRQLWWGQQIPAYYLPNGEYVVESTFEEALEAARRIDPSIKEED
                     LVQDEDVLDTWFSSWLWPISVFDPSKPGFPDKEPNRDLAYYYPTNDLVTAPEILFFWV
                     ARMIMAGQEFCQDIPFRNVYLTGIVRDKLGRKMSKQLGNSPDPIKLMKEYGADGVRLG
                     MLLCTSAGNDILFDESQVEQGRNFSNKIWNAYRLVKGWKVSETKSQSDVNLLAVSWFS
                     AKLNKAIESINEDFTKFRISDAVMTIYKLFWDDFCSWYLEIIKPPFADGAQESIDKAT
                     FDATISYFDSLLKILHPVMPFITEELWQNLEEREKGETIMLRQMPVSTCYDEAIISAF
                     DKAIETVVAIRALRQLKNISPKEPLMVQVRSTYYPEIITPVISKMANVPDLQVVEEFD
                     TTSAGQSFMVGTIEYFVPLEGMINMDEEIAKIESEIKRYEGFIKGVNAKLGNAAFVAN
                     APEKVVALERKKLSDATTKIETLQARLATMK"
     gene            complement(9234..9866)
                     /locus_tag="PLI69_00895"
     CDS             complement(9234..9866)
                     /locus_tag="PLI69_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOG41081.1"
                     /translation="MFEGYRFILASKSPRRHELLAGLDIKFKVETGPETPEAFNCDIP
                     HSQVPEYLAKHKSLAFPGVLEADEVLITADTLVFGSHEKLEIGMLSDEILQKCLILGK
                     PKDREDAVRILSLLSDNTHYVLTGVAIRTPNEIRSFTATTEVTFKKLSMEEISYYIEK
                     YEPYDKAGAYGVQEWIGHIGITAISGSYYNVMGLPVQRLYQELCELCPKK"
     gene            complement(9859..10440)
                     /locus_tag="PLI69_00900"
     CDS             complement(9859..10440)
                     /locus_tag="PLI69_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG41082.1"
                     /translation="MKTENMFENIKAFVFDVDGVFTDGSVLAMPDGDLLRTYNAKDCF
                     AVRMAVDNGYPVAIITGGCSQSIMHRAISLGVEAQDLYQLSRDKRPDFLKFCSDYGLK
                     PSEVAFVGDDVPDIPVLREAGLAVCPSDAVADVKEACNYISPFPGGRGCIRDIVEKVL
                     KMQGRWQFDPEIPVSPNYPDHIMELSKKTGRNV"
     gene            complement(10454..12157)
                     /locus_tag="PLI69_00905"
     CDS             complement(10454..12157)
                     /locus_tag="PLI69_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOG41083.1"
                     /translation="MKKYWGYDSFRPMQEDIITSVLEGRDTLAILPTGGGKSICFQVP
                     SMMRPGICIVVSPLIALMKDQVESLNERGIPSLLVCSGMSYREIDITLDNAVYGDYKF
                     LYLSPERLRTEIFRVRVARMNVNFLAVDEAHCISQWGHDFRPDYLQIAEIREIINTQK
                     RVPVIALTATATLKVADDIMSNLEFDSTNKITSSFERPNLSYVFRKAEDKMGQLLSIC
                     QNVPGSGIVYVGKRRTAEQIASFLRSQKIDAQAYHAGMERDGRSRIQDQWKSNRTRII
                     VSTNAFGMGIDKPDVRFVCHFDMPDTLEGYFQEAGRAGRDGEPSYTVLLWNESDIRRL
                     RQIGRVTFPPLDYICDIYQKVFIFLGIPYEEGRGGSVKFDLDEFVRHFKLHSATAYYA
                     IRYIEQAGYWTLSEELEIPSKVRFDVSRDELYRVQLGSNEMDTFVKVLLRMYPGIFSG
                     YVTIDEEQIARAGRYAVDAVKAKLISLSKRHVLTYVQKFRSPILTFYNERLYEKNLRL
                     PESEYDEKVERRNAGVNAMISIVTDNSTCRSVSLLSYFGQKESAPCGKCDICIDKKKR
                     I"
     gene            12278..>12721
                     /locus_tag="PLI69_00910"
     CDS             12278..>12721
                     /locus_tag="PLI69_00910"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOG41084.1"
                     /translation="MANIDLSKYGITNVKKVIHNPSYEELYKAELDPALEGFEAGQLT
                     ELGAVNVMTGIYTGRSPRDKFWVMDDVTRDTIWWTSDEYLNDNKPVTPEAWKVLKKIA
                     SEQLSDKTLYVMDAFCGANENSRIKIRFIVEVAWQAHFVKNMFIRP"
BASE COUNT         3486 a         2958 c         2762 g         3515 t
ORIGIN      
        1 ccactacggg ggcttttgaa tatttgagga catcccaaaa ggagtcatcg gagatgtgag
       61 atacgtctac cagtatgccg aggcggttgc actcctctat cacctctttt ccgaaggggc
      121 ttacacctcc ccagcgtttc tcctttggag cgcaggagtc acaaatctca ttattgccgg
      181 cgtgtgtcag tgtaagatat cgtactccca tacggtaaaa catcctgagc atggagagat
      241 ccttctgtat cggagcacca ttttccatac cgagaaaaat ggaaataagt ccattctttt
      301 tattgcgttt ggcctgtgcg ggagtggtgg caaaagccac tttgtcggga ttttgctcca
      361 ctgcatcgta aacactcgat ataagttcga aagctcgtct ggtagactcg tccggagaga
      421 gagaattaga ggtgtaaatt gcgaaaaaag agccgttgac tcctcccttt ttcattttgg
      481 caaaatccac gtgtgtaacg ggaaacttgc cgggagtata gccttgctgt actgtctgcc
      541 caaaatctgc acccttgacc agcattagcg gagagtcaca gtgggaatcc aataaaaata
      601 tcataaaatc tcaataatat tttaaggtta gataaataat ggtcaaattt acaaattatc
      661 gagaaattgt gcaatagttg caacaatcgg agccaagctc cgtcttatag ttagaaaaga
      721 gagtgatgac acagaaagag atcaagaagt tttatgacac tcactacagg cgacttttca
      781 atgcgagtta caggatagta ggcaacggtg ccgatgctga agaaatcatg catgatacgt
      841 tgctgagatt tttagaaatg gagaatccgc ctaaggaacc tgctcaaata tctgcctggc
      901 ttattcgcac ctgtataaga aggtcaattg acagactgag ggtcatcaaa agggaaatga
      961 gttttaagga aaactatgaa gaacctgaaa gcatttccgc tgaagagagt gaggagatgg
     1021 agatctccac aagggaggag gtaaaacgcg taaaagaggc actccaaacc atgaaagatc
     1081 cctatagatt ggttttgaca ctctcgctgg ttgaaggaat ggattatcag gagattgcgg
     1141 agattaccgg gcagaaagag gtaacgatac gctcccaggt tagcagagga aaggcagcac
     1201 ttttaaaaat attgcaaaat gagagaaatt gacaacatca ggaagatttt tgacgacatg
     1261 gagccggcag agggccattt tgagaggttt ttgaaaaaag caaatccctc gaaaaggatt
     1321 gaattccgca ggaatactcc acgcaaacga gtattctgga aggcggtact cttccctgtc
     1381 gctgcatcgg tgatgatgat ttttggagcc aatctgctta tcaagaccat gaacgtaaaa
     1441 agtgatagtt tctccaagac cgtagcagtt atggaagatc cgcaggatat ctacaatgcc
     1501 tatatggatg tggtgaggga ttgttatgac tatttctcat cgctgacatc gtatgacccc
     1561 cggaatggtg tcagcgaaca tttcaacact ttggaatgta tcacctcgga acatattcct
     1621 cttttggaag tgcttcctga agagctgagt gaccgggaaa aggcggaaat actcaaagaa
     1681 tactacaacc ggagggttgc aagtgtggtt gagctccgca aatatgttgc atcaatcgta
     1741 cgttgacaca tatatttaat agaaacaata aaaagagatt aacagtaaaa acaacttaaa
     1801 tgaaacgttt tatcctaaca tttgccgcaa ttctgggctt tgtgcttaca agcggcgcac
     1861 aaacagtaaa aagttttgac tttaaagatt tcgaggccct cgatatctcc cattggtttg
     1921 acgtggaggt tgtaaggggt aacacttact ctatcgtagt aactataagt gaggatattg
     1981 aaaaatacct tttggtaaag aattctaaag gaactcttat cataggcctg gacaatccca
     2041 gcaaaagagt tttaagaaat gaagataaaa tagcaaaggc gacaattaca atgcctgtgc
     2101 tcaacaaatt gaccctttcc ggagcctcta aattcaacac ttcggataga tttgactcgg
     2161 gtaagaacag atttgtgatg aaacttagcg gcgccagctt agtctacgga ttggacgtaa
     2221 ctgctaaaga tgctcagatt accataagcg gagcctccac cgtaagagaa ctaaaaggtg
     2281 attttgccaa tgcagacatc aaggtatccg gggcaagtaa gatctatgca aaaataaatg
     2341 cagatgaact tgatgttgag atctccggtg catcaaaagg tgaagtggag ggagagtttg
     2401 aagaggtgga cctcgagtgc agtggagctt ccaaggcaat actttccggt atggctgata
     2461 aggcaatcat agaagggagc ggagtttgca gtatcgtagc agacaacatg agtgccaaag
     2521 atgtgaaaat tgacctttca ggtgctgcca aagcgcatgt atacgtgaca agacgtcttg
     2581 atgtagagtg ctccggcgca tcttcctgca cctacaagac agataagtcc ataagcatta
     2641 catcggagat cagtcgtggg tccagtctcc gccgtgttga ctagacttgc ggcatcctct
     2701 gccttactgc ttcaaacagt actatggcag tggccacgga aacattgagg gaaccgattt
     2761 tacctgacat gggaatcgct gcctctacat tacagagcga aagcacagaa tcggaaattc
     2821 ccttaccttc agaacccatt acgattacgg tcggctgagt gaaatctaca tcgtagattg
     2881 aaccgccggc cttttctgtt gcacacacaa cctgaaaacc ggcatcgatc aagtagtaca
     2941 atggaatacg gaggttggta accttactga caggcactct aagaagagct ccggcagatg
     3001 ttttaatggc atcggcattt atggccgctc cgcctttagc gggcaagatc acaccgctta
     3061 ctccggcaca ttccgcactt ctgactattg caccgaaatt gcgtacgtcg cttacgccat
     3121 cgagcatgag aaaaataggg tttggagact ctgcaagtgc cttttctacg ataacttcat
     3181 agggcagata atcaatttgc gacatatacc cggtaacgcc ctggtgtgaa ccacgacaga
     3241 gtttgttcat cttttcaccc ggaacaaact ggaaagggat ctcgttttgt ttcaccaaat
     3301 cgaataattc cctgaaacgg tcgccttcaa ggccttgctt gaatagaatt ttttcaaatt
     3361 tcctgcctgt tcttattgct tcatatacag gatgcatccc gtagaggtag tgttgctttt
     3421 tttcttccat tttatcaatc tattgtaagg gatgcccatt catccatttt ggtttcaaga
     3481 tccgatttaa gtgcctggta tttgtgcgta agttctacca tatcatcttc cggggtgggt
     3541 gatgcaagaa tggattccac ttgtgcgatc tcttcctcca gagatgatat ttcattttcc
     3601 agccgctgcc ggcgattttt tattttacgt tcctcccttg aaaggtattt ctgttcctcg
     3661 taagtggaga atttttcctt tttggactca ggagccggct gagcagaaga ttgctgcttg
     3721 atttctattt ccctgaggga acttatcttc tttctataaa ggaagtcatc tacacctccg
     3781 aggtgctcct ttacccctcc gtcagcaaac tcatatattt tctccaccag tccatcaagg
     3841 aaatctctgt cgtgagaaac gatgatcagg gtgccgtcat atttctgcag cgcttgtttc
     3901 aacacatcct ttgagcgaag gtccatgtgg tttgtaggct cgtccagagc caatagattg
     3961 taaggatgca gaataagttt tgccatggcc agtctggagc gttcacctcc gctaagtacc
     4021 gccaccttct tatctatatc gtcacctctg aaaagaaaag cacccagtat atcgcgaagt
     4081 ttggttctta tatctccaac tgcgatcttg tccaaggtac catatacagt atcttttttg
     4141 tccaataaat cctcctggtt ttgcgcaaag tatccggtct ttacattata accaacaaga
     4201 gcttcacctt cgaagggctg gagttgaccg gtgagtagtc gcataagagt agtcttaccc
     4261 tcgccattcc ttccgacgaa ggccaccttc tcccctctcc ttattgtcat gtttgctccg
     4321 cgcaatacta tcttcttcct gggcttggaa tatcctatag ttgcatcctt aagggaaaat
     4381 accacatctc cggagcgcgg tgcagaggga aaacgaacgg taatggtgtt caaatcctct
     4441 tcatctatct cgatgcgctc caacttctcc agttgtttaa tgcgggattg gacctggttg
     4501 ctcttggtag gcttgtagcg aaatttttct ataaactcct cgctcttttc tataagacgc
     4561 tgctggttgt cataagcggc acgctgctgg gcaatcctct cctttctcag ttccacaaat
     4621 tgcgagtagg gtactttata gtcatgaatt tttcccagca tcacctctac ggtacgattg
     4681 gtacagttgt cgaggaatct gcgatcgtgg gagatcagca ccaatgcgcc tggaaaggtt
     4741 ttgaggtact cttcgagcca ctgaatggat tcgatgtcga gatgatttgt cggctcatcc
     4801 aacagcagta tatcgggatt gcgaagcagg atttttgcca gttcgatgcg catattccag
     4861 ccgtggctga atgtgtttgt catcctgtcg agttctcctt tgcgaaagcc aagtccgaga
     4921 agggttttct cggcctgcac atggggagat tccaaggggt aaagcctgag ttgatcactt
     4981 atttcattca gttcaattat cagtctgtgg tactcatccg actcgtagtc tgtgcggctt
     5041 gccaactcat gcgatatccg gtccatccgc atattgagct cattatgctc ggcaaatgcc
     5101 gtcatagcct cctcaattac gcttttgtcg cgtgaatgct tcattatctg tggaagatag
     5161 cccactttcc tatctgccgg catcgagacc ttacccgatg tgggcgactg aaggcccatt
     5221 atgagtttta gaatggtgga tttaccggct ccgttcttgc cgacaagtcc tactttctct
     5281 ttctcgctaa tgtgaaaatt tatgctgtcc agaagagtaa atcccccgaa tgagacagtg
     5341 agatcactga tcgatatcac tcctcacctc ccggggcatc agccgcaatt tcatccgttt
     5401 cggatttgcc cgacctgcag accagaatac taacctcata aagaaaatag agtggaattg
     5461 ccacaaataa catcgtcact gcatctccgg aaggagttat aagtgccgca agaatgagaa
     5521 gtatcaccag ggcatgttta cggtatttct taagaaactc ttttgttatg attccaagtt
     5581 tggagagtac tgccgcaagt gccggcattt caaaaacaat tcccataatt agaatcagtg
     5641 agacgaacat ccctatgtaa gaattgagcg atatatggtt agggacatcg gctgagacct
     5701 gataagtgcc gagaaatctt acagtcagag gaaagaccaa taggtatcct actgctatcc
     5761 caatatagaa caaaatcccc gcaaatccaa atgcccgcct tataaccctt ttttcattct
     5821 catagagcgc cgggcgcaca aatgtccata tttggtaaaa gataaagggc atcgctacaa
     5881 tcaatgccac ataaagagat atcctgacat gagtaaagaa ctgagccgcc atctcaatgt
     5941 ttatcagttc aatcgaaaac tcccctatcg gttccgaacc cataaggacg agcagggcat
     6001 ccaaccatcg atagagacag aaatcagatg tcaggggcgc aaatatgatg ttaccgaaaa
     6061 tcgtctcctt gaaaaagaag gcgacgatca tcagcaccac aagcactgcg actgaacgaa
     6121 aaagtacttt ccgcaactca tcgagatgct cccaaaagga catattgctg tttcccatca
     6181 gaagagcagt atggaatggt tatttcttct ccttatcgcc attcttgggt tcatcgtcta
     6241 ttcccttcac ctcattttcc acttccttca tgccctcctt gaaactacgg acgccctttc
     6301 caaggccctt cataagttct ggaatcttct tgccacccca aaaaagcagt acgataaagg
     6361 cgatgatcag gatttcctgc catccgaatg atccgaataa taacaaattt ctcatcttat
     6421 attggtatta taatattttc aacactattt catagtggcc aggcgcgcct gtaatgtctc
     6481 tattttggta gtcgcatcgg agagtttttt tcgctccaga gctacgacct tttcgggagc
     6541 atttgccaca aatgccgcat tacccagttt tgcattcact cctttgataa aaccttcgta
     6601 acgtttgatc tcggattcta tctttgcaat ctcttcatcc atattgatca taccttccag
     6661 gggcacaaaa tactctatag tacccaccat aaaagattgt cctgccgatg tggtatcaaa
     6721 ttcttccaca acttgtaaat cgggcacatt cgccatcttg gaaattaccg gagtaatgat
     6781 ttcagggtag taagtactcc tgacctgcac cattagcggc tctttgggag agatattttt
     6841 taactgtctt agtgccctta tggctacgac agtctcaata gccttatcga aagcagaaat
     6901 aatggcctca tcatagcagg ttgaaacagg catctgccgg agcatgatgg tttccccttt
     6961 ttcacgttct tctaaattct gccatagttc ttcagtaatg aaaggcataa cgggatgcag
     7021 tatctttaaa agagaatcaa aataggagat tgtagcatcg aaggtagcct tgtctatgct
     7081 ctcctgagca ccatctgcga atggaggctt gataatctcc agataccatg agcagaaatc
     7141 atcccagaag agtttgtaaa tagtcattac tgcatctgaa atgcggaact tggtaaaatc
     7201 ttcgttgata ctctcaatag ccttattcag tttggcggaa aaccaggaga ctgctaggag
     7261 attgacatcg ctctgactct tagtctcact aaccttccag ccttttacca aacgataggc
     7321 attccagatc ttattgctga aattacgtcc ctgttctacc tgactctcgt cgaaaaggat
     7381 gtcgttgccg gcagatgtac agaggagcat tcccaggcgc acgccgtctg ctccatactc
     7441 cttcattaat ttaatgggat caggagagtt gccaagctgt tttgacatct tacgacccaa
     7501 tttatcacgc actatacccg tcaggtagac atttctaaaa ggtatatcct gacagaattc
     7561 ctgacctgcc atgatcatcc tcgcaaccca gaaaaagaga atttcagggg ccgtcacaag
     7621 gtcatttgtc ggatagtagt atgcgaggtc gcgatttggc tccttatcgg ggaagccggg
     7681 cttggaaggg tcaaataccg atatcggcca tagccaggat gagaaccatg tatccagtac
     7741 atcctcgtcc tgaaccagat cctcctcttt tattgaaggg tcgattcggc gtgcagcctc
     7801 cagggcttct tcaaatgtag attcaaccac gtattcccca ttgggaagat aataggccgg
     7861 aatctgctgt ccccaccata actgtcgtga tatacaccag tcacgcacat tttccatcca
     7921 gtgacgatat gtattcttga atttatcagg gatgagtctc acggagtcat cttccaccac
     7981 ttgtagggcc tcttgtgcaa gatcctccat tttaaggaac cactgcatgg agagttttgg
     8041 ctcaataacg gcatttgtac gctccgagta acccacctgc gacttgtagt cctccacctt
     8101 ctcgagaagt gcggcctctt cgagcatttt aactatcttt ttacgggcga cgaatctctc
     8161 ctctcctaca aggatttgag cttgttcatt gagggttccg tcatcgttga taatgtcgat
     8221 tacagggagt ttgtgacgca agcctatctc gtagtcattg atgtcatggg ccggggtgat
     8281 tttaagggct cctgtgccga ggtccatcgt cacatactcg tcctcaatta taggaatctc
     8341 tttattgata agcgggataa tcacattttt tccacgcaga tgaagatatc tttcatcttt
     8401 gggattgaca cagaccgcca cgtcagccat aatggtttcg ggacgggtcg tggcaatagt
     8461 gacataatca cactctcctt gtgagacacc acctgctatt ttatacctca ggtagtagaa
     8521 tttggagtcg gtctccctat gtattacctc ctcgtcgctt accgccgtca aaccttcggg
     8581 gtcccaattg accatacgca ctccgcggta gatgagtcct tttttataga aatggacaaa
     8641 tgtattgatg acggcagcac ttagatcggg atccattgta aatctggtac gattccaatc
     8701 acaggaggct ccaagctttt tcagttgctc cagaatgata ccaccatgtt tatccctcca
     8761 ctcccaggca tgctttaaaa attggtcacg tgtcaggtcg gacttctcta ttccctcagc
     8821 tttgagcttt tccaccacct tcgcctctgt tgcgattgaa gcatgatccg tacccggaac
     8881 ccagcaggca ttgtagccgg tcatcctcgc acgacgcacc aacacatcct gaagtgtatt
     8941 gttgagcatg tgacccatgt ggagcactcc ggtcacattg ggaggcggga tcactatagt
     9001 atatggttca cgttcatccg gttccgagtg gaaaaatcca ttctcaagcc agtaagaata
     9061 ccacttgtct tccgtaaggg aagggtcgta tttgttgggg agttccatct atctaaatat
     9121 atagctttgt tgtggtcaat tattcaaact gcaaaaataa acaatttcgg taaattatcc
     9181 ctaagaatga gaacagcatc tcgctaaatg gtcgcctaat ctctttagta taatcacttt
     9241 ttaggacaca gttcgcacag ctcttgataa aggcgttgta cgggaagtcc cattacattg
     9301 tagtaagagc cgctgatggc ggtaatgccg atatggccta tccactcctg cacaccgtaa
     9361 gccccggctt tatcgtacgg ctcatatttc tctatataat aggaaatctc ctccatggag
     9421 agctttttga aagtcacttc tgtagttgcg gtaaatgaac gtatctcatt gggtgtacgg
     9481 attgccactc ccgtcagcac atagtgggtg ttgtccgata gtagagagag gatccttact
     9541 gcatcctccc tatctttcgg tttacccaat atcagacatt tttgaagaat ttcgtcagag
     9601 agcattccga tttccagttt ttcatgggaa ccgaagacaa gcgtatcagc tgttataagc
     9661 acttcatcag cctccaggac tccgggaaat gcaagggatt tgtgtttggc gagatattcg
     9721 ggaacttgtg agtgcggaat atcgcagttg aaggcctccg gcgtttcggg acctgtttcc
     9781 actttgaatt tgatatccaa tcccgccaga agctcgtgcc ttcggggcga ctttgaagca
     9841 agaataaatc tataaccctc aaacatttct tcctgttttc tttgataact ccattatatg
     9901 gtcgggatag ttgggactta caggtatttc gggatcaaac tgccatcttc cctgcatttt
     9961 cagaaccttt tcaacaatat ctctgatgca tccccggcca cccgggaaag gagaaatata
    10021 gttgcatgcc tcttttacat cagccaccgc atcggatgga cagactgcaa gaccggcctc
    10081 acgaagtacc ggtatatccg ggacatcatc tccgacaaat gccacctctg agggtttcag
    10141 accataatct gagcaaaact taaggaaatc gggacgcttg tctctcgaaa gctgataaag
    10201 atcctgagct tccaccccca gtgaaatagc cctatgcata atactttgtg agcaacctcc
    10261 tgtaataatc gctaccggat agccattatc cactgccatt cttactgcaa aacaatcttt
    10321 tgcgttgtat gtgcgaagaa ggtccccgtc cggcatcgcc aaaacagaac catcggtaaa
    10381 aactccgtcc acgtcaaata caaatgcttt aatgttttca aacatatttt cagtcttcat
    10441 ttattgttat tctttatatt ctcttttttt tgtcaataca aatatcacat tttccacagg
    10501 gggcagattc cttctgaccg aaatagctca gcagagatac gctgcgacag gtggagttgt
    10561 cggtaacaat ggatatcatg gcgttcaccc cggcgttgcg gcgctccact ttttcatcat
    10621 attccgactc gggcaaccgt aggttttttt catacaaacg ctcattataa aatgtaagaa
    10681 taggcgaccg gaatttttgc acataggtca aaacgtgtct ctttgacaga gagatgagtt
    10741 tggccttgac cgcatccacg gcataacgtc ctgccctggc aatttgctct tcatctatag
    10801 tcacatagcc ggaaaaaatc cccgggtaca tcctcaaaag tactttcaca aaggtatcca
    10861 tctcattgga accgagctgg actctgtata gttcatccct cgaaacgtca aaacgcacct
    10921 ttgagggtat ttccaactct tcggaaaggg tccagtatcc cgcttgctca atgtatctga
    10981 tcgcataata ggcagtagcg gagtggagtt tgaaatgccg cacaaattca tcaagatcaa
    11041 acttaacact acctccccga ccctcctcat agggtatccc cagaaaaatg aaaaccttct
    11101 ggtaaatatc acagatatag tccagaggag gaaaagttac gcgaccaatc tgtcgcaaac
    11161 gtctgatatc gctttcattc caaagcaata ccgtataaga tggctccccg tccctgccgg
    11221 ccctgcctgc ttcctggaaa tatccttcca gagtatcagg catgtcaaaa tggcaaacaa
    11281 atctcacgtc gggcttgtca atgcccattc cgaaggcgtt ggtggatact attatacgag
    11341 tccggttgct cttccattgg tcttgaatac gcgagcgtcc atccctttcc attccggcgt
    11401 gataagcctg tgcatcaatt ttttgactac ggaggaaaga agcaatctgt tcggcagtac
    11461 gcctcttgcc tacatagact attccactcc ccggcacatt ttgacatatt gagagcaact
    11521 gtcccatttt gtcttcggct ttccggaaaa cataggaaag attaggcctt tcaaaagatg
    11581 aggtaatctt gttggtagag tcgaactcca gatttgacat aatgtcatcc gcaaccttca
    11641 aagtggcagt agcagtaaga gctatgacag ggacacgctt ttgtgtgttg attatctctc
    11701 taatttccgc aatctggaga taatccggcc tgaaatcatg gccccattgg ctgatacagt
    11761 gtgcctcatc caccgccaga aaattgacat tcatccttgc caccctcaca cggaaaattt
    11821 cggttctcag gcgttcagga gagagataga gaaacttata gtcaccataa acagcattgt
    11881 cgagagttat gtcaatctcg cgatatgaca taccggaaca gactagtaat gaggggatac
    11941 cgcgttcatt aagactctcc acctgatctt tcatcagcgc tatcagtggc gataccacta
    12001 tacatattcc gggtctcatc attgagggca cctgaaaaca aatagacttg cctccgccgg
    12061 taggtaaaat ggcaagcgta tccctcccct ccagcacaga ggtgataata tcctcctgca
    12121 tcggacggaa ggagtcatat ccccagtact ttttcaaagt ttctctcatt gcgcaaaaat
    12181 agtaattata tggcaaactt tttgcgtaaa attaggggca tataaataat tattatcttt
    12241 gcccgacaaa taataaaaag aaataaaaat aacaattatg gcaaatatcg atttatctaa
    12301 gtacggcata accaatgtca aaaaagtaat acacaacccc agttatgagg agttgtacaa
    12361 agctgaactt gatcctgctc tagaaggatt tgaggcgggt caattgacgg agcttggtgc
    12421 cgttaacgta atgacaggta tctatacggg acgttctccc agagacaaat tctgggtaat
    12481 ggatgatgtt acgagagata cgatttggtg gacttcggat gaatacctta atgacaataa
    12541 gccggttact ccggaagcat ggaaggtgct gaaaaaaatt gcttcagagc aactctcaga
    12601 taagactctg tatgtgatgg atgcattttg cggtgctaat gaaaactcac gcattaaaat
    12661 ccgtttcata gtagaagtgg catggcaggc acatttcgta aagaatatgt tcatacgccc
    12721 t
//
LOCUS       DAOSIE010000012        12305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_5176_length_12305_cov_4.885796, whole genome shotgun sequence.
ACCESSION   DAOSIE010000012 DAOSIE010000000
VERSION     DAOSIE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12305
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5176_length_12305_cov_4.885796"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /gene="sufB"
                     /locus_tag="PLI69_00915"
     CDS             <1..895
                     /gene="sufB"
                     /locus_tag="PLI69_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOG41085.1"
                     /translation="SAVFSDGSFCYIPKGVRCPMELSSYFRINAKGTGQFERTLIIAD
                     EGSYVSYLEGCTAPIRDENQLHAAVVEIIVERDAEVKYSTVQNWYPGDKEGKGGIYNF
                     VTKRGLCKGSGSRLSWTQVETGSSITWKYPSTILRGDNSSSEFYSVAVTNNRQQADTG
                     TKMIHLGKNTVSRIVSKGISAGYSQNSYRGLVKVAPGASGSRNHSQCDSLLLGDKCGA
                     HTFPVIQCDNLSSVVEHEATTSRISEDQLFYCRQRGIPEEDAVGLIVNGYAREVLNQL
                     PMEFAVEAQKLLQISLEGSVG"
     gene            892..1566
                     /gene="pnuC"
                     /locus_tag="PLI69_00920"
     CDS             892..1566
                     /gene="pnuC"
                     /locus_tag="PLI69_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOG41086.1"
                     /translation="MIKEIINYPLFTIAGDLPVLLIDLVTAIFGLTCVFLAGRNSKYN
                     FWVGYFYTALLFLMFWNKNLYASLILQPISLGINILGHYRWTHPKKGEESSSDPKSLK
                     VTMLTWRQRTITIALVFLLAIVWGWVLRQLGTRWFVGVFPSDPIPYLDAFVTVLILVA
                     QFLSAQKKWDCWVVWLFVNITQIILHISVGHIFMPIVSGLYLINGLASLYTWFKLYKK
                     EKNDNK"
     gene            1581..2321
                     /gene="sufC"
                     /locus_tag="PLI69_00925"
     CDS             1581..2321
                     /gene="sufC"
                     /locus_tag="PLI69_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOG41087.1"
                     /translation="MLKISGLKVSIDDREILTGVNLEVRPGEVHAIMGPNGSGKSTLS
                     AVLAGNENFKVTEGEVLFEGEDLLKLSPEERSWAGVFLGFQYPIEIPGVTNVNFIKTA
                     INEHRKRKGLPELSAAQFLKLMRERMAYVDMDSSFTSRGVNVGFSGGEKKRNEIFHMA
                     MLEPKLAILDETDSGLDVDALRIVASGINRLKTPQNATIVITHYQRLLDYIVPDYIHV
                     IYKGSIIKSAGRELAREIEQRGYDWLIN"
     gene            2324..3325
                     /gene="sufD"
                     /locus_tag="PLI69_00930"
     CDS             2324..3325
                     /gene="sufD"
                     /locus_tag="PLI69_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOG41088.1"
                     /translation="MKQNYIYAPNLTSEFKCKVPLQGTRQLFIVNGRVQGGRSPFSLR
                     LEEGEAMEEILQIISVRHKSYKEELSTQQDFHFGKGSVGRIIQCSHTFSPDRFVTTEK
                     VTITVEKDASAEFCIMQNEHNNAEHNTLYEANLAEGASLNMVFLSLHGGIIRNEIVVN
                     LNGSHAQCDLAGLYLTDSRQLMDNNIHLNHLVPYCTSSQLFKGILDDEGVARFYGLIK
                     VAPDAQKTEAYQANHNLLISDKAKAHTKPQLEIYADDVKCSHGATMGRLNEDELFYMR
                     TRGIPAAEARILQQKAFAHEVLEKISNTQLRERMLILVERRLRGEFSHCKNCTNNCC"
     gene            complement(3322..4332)
                     /locus_tag="PLI69_00935"
     CDS             complement(3322..4332)
                     /locus_tag="PLI69_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41089.1"
                     /translation="MGTTRTEISTIGRENLINRLFENTGYTNERTIKLSEKGECCTSH
                     KILLEGVNFDLVYTPLKHLGYKAALCAMGEIYALLRQPASLSLNLGLSSRFSVEDVTD
                     LWSGFVAAAKEHAIKHLSLELNPSLTGLCISISSSGVQKKGIIAKVPAPKNMDLICLT
                     GNVGAAYMGLHVLQREKVSFIEESSQQPDLSKYKHLLAAYLSPEINPSVVSRFLKEEI
                     YPSAGYFLTRGLGAGVLDLVKDSGFGAKIYLDRIPVSSQSLEMAQEIEMDMVTAIING
                     GEDYKFIFTVPIEKHDVIRKEFQDYDVIGHLARPEVGAVLVTPEGAELTIRAQGYEAS
                     EG"
     gene            4572..7094
                     /locus_tag="PLI69_00940"
     CDS             4572..7094
                     /locus_tag="PLI69_00940"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOG41090.1"
                     /translation="MSIMKYSFDEAIAASKIYFKGDELAANVWVNKYALKDSFGNIYE
                     KSPADMHKRLAAEFARIEKKYPNPMKASEIFELLKDFKYIIPQGGPMTGIGNEHQVAS
                     LSNCFVIGHERPADSYGGIMMIDEEQVQLMKRRGGVGHDLSHIRPTGSAVLNSALTST
                     GVVPFMERYSNSTREVAQDGRRGALMLTISIKHPDAENFIDAKMEQGKVTGANVSVKV
                     DDDFMRAALSGSNYTQQYPVYSKKPKVTQEIDASSLWKKIIHNAWKSAEPGVLFWDTI
                     LRESVADCYADKGYRTVSTNPCGEIPLCPYDSCRLIAINLYSYVDHPFTPKASFNFPL
                     FKEHCVKAMRLMDDLIDLEMEKIDLIMAKIKADPEDDYVKRTEYELWDKISKKSLGGR
                     RTGLGITAEGDMLAALGLTYGTMEAIEFAVQVQKTLAVEAYRSSVIMAEERGAFPIYE
                     ASKEVNNPMIIRIKTEDPDLYARMVKSGRRNISMLTIAPTGTTSLMTQTTSGIEPVFR
                     PFYLRRKKVNPNDVNVKVSYTDDMGDSFEEYFVFHHKFLTWAMINGYERDQVLGMCED
                     ELEDLLKKSPYYKATANDIDWVSKVKMQGEIQKWVDHSISVTVNLPEDITEETVAEVY
                     KTAWNSGCKGITVYREGSRSGVLVSTSKDTPVLKPKKRPQTLEADIIRFRNKSEKWIA
                     FVGKLNGQPYEIFTGIVDEDTRVIPRSIEKGWIVKEKDLKSGKSRYDFHYVDALGYPS
                     VLGGISHMFNKEFWNYAKLISGVIRNGMPIVDILNLVQGLELDTESINTWKNGVERAL
                     KRYIPDGTRDETGQKCSNCTSKNLVYQEGCLVCLECGYSKCS"
     gene            7121..7876
                     /gene="ispE"
                     /locus_tag="PLI69_00945"
     CDS             7121..7876
                     /gene="ispE"
                     /locus_tag="PLI69_00945"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOG41091.1"
                     /translation="MIFFPNAKINLGLDIIEKRPDGFHNIETLFVPHTECYDILEVLY
                     AEKFSIHLYGIPLEGDPMDNLCVKAFRLMEKIYDIPPVSIRLYKKIPVGAGLGGGSSD
                     AAGTLILLNRMYSLGLSDEVLAGHAASLGSDCPFFIYNRPMYATGRGEMLSPVELALD
                     DYRIELVTPDIFISTKEAYSGVTPRRPAVPISEIIKKPVEEWKGFLKNDFEESLFLRY
                     PRLAAEKEALYARGAVYASMSGSGSSLYGLFKK"
     gene            7907..8518
                     /locus_tag="PLI69_00950"
     CDS             7907..8518
                     /locus_tag="PLI69_00950"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOG41092.1"
                     /translation="MHIGIAGNIGSGKTTLTRMLAQHYGWTPKYEAVTYNPYLEDYYK
                     DIPRWSFNLEVYFLTQRIKDLMDIANSDKVIIQDRTIFEGVYVFVANNKDMGNLSQRD
                     FDAYMDLFGVMMKMIKVPDLLIYLRSSIPHLVSRIQKRGRDYEQSMSLEYLGGLNERY
                     EKWISEYPGEVLIIDADNLDFENRPEDFASITNRLDARLFGLF"
     gene            8613..9230
                     /locus_tag="PLI69_00955"
     CDS             8613..9230
                     /locus_tag="PLI69_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41093.1"
                     /translation="MTYLEFRAQLFDLACFNIHQVYAWQPDFDRNNLTRWVKKGYLIR
                     LRQGYYAFSEYKGKPDYPLYFANRIYRPSYISLHTALSFYGMIPEAVVQITSVTSLKT
                     ASFANDFGEYSYKNVKENLMFGYDLKQMVDNRTIRFATPEKALLDLLYLYPFYNTERE
                     LEELRLDDYFMHEDLNEDLLMVYCEKFQSKALDNRIGLLFKAYDL"
     gene            9227..10009
                     /locus_tag="PLI69_00960"
     CDS             9227..10009
                     /locus_tag="PLI69_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOG41094.1"
                     /translation="MIRIEQIRNFFPAQIRRDSGFDKYMLKEYLQLMILDYLSSTPSI
                     RKMAFIGGTNLRLVKGIDRFSEDLDFDCKEFSKEEFDEMTNGVVQFLERSGLRVEVRD
                     KENPKLTAFRRNIYFPELLFDLGLSGHKEERFMIKIESQDQGISYPHVMTNIKGCGFF
                     FPFPVPSDGVLCSMKIAAMLSRAKGRDFYDLMFLLPQSKPDYDFLSKRCGIHNLQEFK
                     QATSELLKTVDLNKKQKDFEHLLFNKTSSEKILRFGEFVDSL"
     gene            10020..10688
                     /locus_tag="PLI69_00965"
     CDS             10020..10688
                     /locus_tag="PLI69_00965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOG41095.1"
                     /translation="MMSKTYIHPRLGEIIIRKSRLSRRVKLIVHPINGISVTVPWFVS
                     YSKAIAFVEEKEEWIAATLLKQRKKRKEEAVRIAPDQPLKLITGEIPYERLKMASSDG
                     IISEEDIVKIIRAEAKKYLPQRVELLAAKYGFNPGKVTVRNNRTNWGSCSKSGNISLN
                     IHLMRLTPELADFVIVHELCHLIHKNHGPQFHALLNSLCNGRERELNRLLKQQRPQVL
                     FVTE"
     gene            complement(10734..>12305)
                     /locus_tag="PLI69_00970"
     CDS             complement(10734..>12305)
                     /locus_tag="PLI69_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG41096.1"
                     /translation="NIFAGASANIPLSKERASLLLSGRYSPFQLEYKALKSFLPKEKL
                     PFDKLQTNIFDLYGKFSYRIDDASRLEFFLFATEDSYGFGKEDLSKNYLRWGNIVSKA
                     TYNRIMKSGRLYDASLAYNRFSSGQRQEVYMEGTFNELAIRSGLNEITLNLKTEKERH
                     GHSFITGLKLMYSLNAPGSSKLYEGEGRNVEKKAYVRNPHHSILNTLYFQYSTTAIRN
                     LFFKLSLKASLYAPDLQSELNTERGVLFRPEGSLLLSYIFSKYLQLEASVDRTVQFYH
                     VLEGLPIGWSMDLMVPAGKKNKPEESTQFYLGLSSTPGNHFVSLGGYYKFMNNLVFYT
                     DATTLFSASLSGWSDNIEMGKGTSYGGEFMYRYTAPKTHIRVAYTLSKTDRTFDNINY
                     GESFPAKFDRRHILNLSASYGGFNSAFTLQSGHWESVKTSVYPGVLPDGSPIILDYFG
                     RPNNYRMPTYIRWDIGYSFKWERPKVKHYLNVGIFNVLNRHNAFTLWYDSENNNWKQL
                     SLFPIMPSISYRITF"
BASE COUNT         3364 a         2730 c         3020 g         3191 t
ORIGIN      
        1 ctccgcagtt ttcagtgacg gctccttctg ctatattccc aaaggggtca ggtgtccgat
       61 ggaactttcg agttatttcc gcataaatgc gaaaggcacc ggacagtttg agaggactct
      121 gatcattgct gatgaaggct cctatgtgag ctatctggag ggctgtacgg caccgatccg
      181 cgatgagaat cagctgcacg cggcagtggt agagatcata gtggagcgcg atgcggaggt
      241 aaaatattcc accgttcaga attggtatcc gggagataag gagggcaagg gaggtatcta
      301 caattttgtg acaaagagag gcctctgtaa gggttcgggc agccgtttgt cctggacaca
      361 ggtggaaacg ggatcctcca ttacctggaa atatcccagt accatactta ggggagacaa
      421 ttccagctcg gaattctatt ccgtagcggt gacgaacaac cgccaacagg cagacacagg
      481 taccaaaatg atccatttgg gtaaaaatac cgtcagtcgc atcgtaagta agggtatctc
      541 ggcaggttat agccagaata gttaccgtgg gcttgtaaaa gtggcccccg gtgcttccgg
      601 atcgcgtaat cactcccagt gcgactccct tttgttggga gacaaatgcg gtgctcacac
      661 cttccctgtg atacagtgtg ataatctaag ctcggtagtg gagcacgagg ctaccacctc
      721 ccggataagt gaagatcagc tcttctattg tcgtcagaga ggcatcccgg aggaggatgc
      781 ggtaggactt atcgtaaacg gttatgcgag agaggtgctg aatcaactcc ccatggagtt
      841 tgcagtcgag gcgcaaaaac tattgcaaat atcacttgaa ggaagtgtag gatgattaaa
      901 gagataataa actatccgct atttaccatt gccggtgatc ttccggtgct gcttatcgac
      961 cttgtcaccg cgatattcgg gttgacttgt gtctttctgg ccggtcgcaa cagcaagtac
     1021 aacttctggg tgggctattt ctatacggca cttctctttc tgatgttttg gaataaaaat
     1081 ctctacgcca gcctcatact tcagcccata tctttgggta taaatattct gggccactat
     1141 aggtggaccc atcctaaaaa aggggaggag agttcttcag accccaaatc ccttaaggtg
     1201 accatgctca cctggcgcca gaggacaatc accatagcat tggtcttcct tctggcaata
     1261 gtatggggat gggtgctgag acagcttggt acaagatggt tcgtgggagt gttcccttcc
     1321 gacccaatac cctatctgga tgcatttgtg acggtattga tacttgtggc acaatttctt
     1381 tccgctcaaa agaaatggga ttgctgggtt gtctggcttt ttgtcaacat cactcagata
     1441 atacttcaca tctcggtagg ccacatattc atgcctattg tgagtgggct atacctcatc
     1501 aatggtttgg cctccctcta tacttggttt aaattgtaca aaaaagaaaa aaacgataat
     1561 aaatgaacaa cgacaacatt atgcttaaaa taagcggact aaaagtctcc atagatgacc
     1621 gtgaaatcct cacaggcgtc aatctggagg taaggccggg agaggtacac gccattatgg
     1681 gtccaaacgg ctccggcaaa agcactcttt ccgcagtact tgcgggtaat gagaatttca
     1741 aagtgaccga gggggaagtc cttttcgagg gtgaggattt gctcaaactc tctccggaag
     1801 agcgctcctg ggcaggcgtt ttcctgggat tccagtatcc gatagagatc cccggagtga
     1861 caaacgtcaa ttttataaaa actgccataa atgagcaccg caaacggaag ggacttcccg
     1921 aacttagtgc ggcgcaattt ctcaaattga tgcgggagag aatggcttat gtcgatatgg
     1981 atagcagctt cacctcaagg ggagtcaacg tgggattttc cggaggagag aaaaaacgaa
     2041 acgagatatt ccacatggcg atgctcgagc cgaaacttgc aatcctggac gaaacggact
     2101 ccggactgga cgtggatgca cttcgcatag ttgcaagcgg catcaacagg ctaaaaaccc
     2161 ctcagaatgc gacaatagtt ataacacact accagcgtct tctcgactac atagtacccg
     2221 actacatcca cgtcatttac aaggggagca tcatcaaaag tgcaggccgt gaacttgcac
     2281 gggaaatcga acaacgaggt tacgactggc tgatcaattg acaatgaaac agaactatat
     2341 atacgcaccc aatcttacca gcgaatttaa atgtaaggtg cctctgcaag ggacacggca
     2401 actctttatt gtcaatggaa gggtacaggg cggtaggagc ccattctcac tcaggcttga
     2461 agagggagaa gcgatggaag agatacttca gatcatctcc gtgcgccaca agtcctataa
     2521 agaagagctc tccacgcagc aggacttcca tttcggaaaa ggctccgtgg gtagaataat
     2581 ccaatgctct cacaccttct caccggacag attcgtgact actgaaaagg ttaccataac
     2641 ggtggaaaaa gatgcatcag ctgagttttg cattatgcag aatgaacaca acaatgcgga
     2701 acacaacacc ttatacgagg caaatctggc tgagggagcc tctctcaata tggtctttct
     2761 ctcgctgcac ggcggaatca tacgcaacga gatagttgtc aatctcaatg gaagtcatgc
     2821 gcaatgcgac cttgcaggac tctacctcac cgattccagg cagctgatgg acaacaatat
     2881 ccatctcaac cacctggtcc cctactgtac cagcagccaa ctcttcaagg gaatcctcga
     2941 cgacgaagga gtagcccgct tctatggtct tatcaaagta gcgccggatg cacagaagac
     3001 ggaggcctac caggccaacc acaacctgct tatttccgat aaggcaaagg cacacaccaa
     3061 acctcagttg gaaatctatg cagatgatgt aaaatgcagc catggggcca ccatggggcg
     3121 cctgaatgaa gacgaactct tctatatgcg tacccgagga atccctgcgg cagaagcgcg
     3181 catccttcaa cagaaggcat ttgcacatga agtgctggag aaaatttcca acactcaact
     3241 tcgcgaaagg atgctcattt tggtggaaag aaggcttcgc ggtgaattct cacattgtaa
     3301 gaactgtacc aacaactgtt gttagccctc tgaggcttca tagccttgcg cacggatggt
     3361 gagttcagct ccctccggag tgaccagtac cgctcccact tcggggcggg caaggtgtcc
     3421 tatgacatca taatcctgaa actcttttct gatcacatcg tgcttttcga taggcacggt
     3481 gaagatgaat ttgtagtcct ctcctccgtt gattatggcc gtcaccatat ccatctctat
     3541 ttcctgagcc atttcgagac tctgcgagga tacgggtatc ctgtcgaggt aaattttagc
     3601 tccgaagccg ctatctttca ccagatccaa tactccggca cccagacctc tggtcaggaa
     3661 atatcctgcc gaagggtaga tctcctcttt cagaaatctg cttaccacgg agggattgat
     3721 ttcagggctt aggtaggcag ccagcagatg tttgtatttt gagagatcgg gttgctgcga
     3781 actctcttca ataaaggaga ctttctccct ttggagtacg tgaagtccca tataggcagc
     3841 cccaacattg cccgtaaggc agataaggtc catatttttg ggtgcgggaa cttttgctat
     3901 gatgcctttt ttctggactc cggaggagga gatgctgatg cagagtcccg tgagtgaagg
     3961 gttgagctcc agagagagat gttttatcgc atgttccttg gctgctgcca caaagcccga
     4021 ccagagatcg gtaacatctt ctacggaaaa tcggctcgag agtccgagat tgagcgagag
     4081 tgaggccggt tggcgaagca gcgcatagat ttctcccatg gcgcagagtg cggctttata
     4141 tccgagatgt ttgagagggg tatagaccag gtcaaaattg accccttcca gtaggatttt
     4201 atgagaagtg cagcactccc ctttttcaga gagttttatg gtgcgttcat tggtgtatcc
     4261 ggtattctca aaaagacggt ttatgaggtt ttctctgccg atggttgaga tttcagttct
     4321 tgttgttccc attggatgta gaaattttta caaaagtaca aaaaaatgta acagtaatgt
     4381 aatggcaaga aatataggac tttgttaaaa ttgttgataa ctttgttgat aactatgttg
     4441 atattactgg aaaggcctat gcattttgct gtaacttgcg tacctgaatt tacagatgta
     4501 aaccggtgtt attggccccg taataacact tgaggggatt tatccccttt cacctagcgt
     4561 aacaactaca aatgtcaatc atgaagtact cttttgatga agctattgct gcgagtaaga
     4621 tttatttcaa gggcgacgaa ctggccgcca atgtatgggt aaataaatat gccctgaagg
     4681 attcttttgg taatatttac gagaaatctc ccgccgatat gcacaagcgg ttggctgcgg
     4741 agtttgccag aatagagaag aagtatccta atccgatgaa ggcatcagag atttttgagc
     4801 tgctcaagga tttcaaatat atcattcctc agggaggtcc catgaccggc atcggtaatg
     4861 agcatcaggt ggcttcgctc tccaattgct ttgtgatagg tcacgagcgt cctgcagatt
     4921 catacggcgg cataatgatg attgacgagg agcaggtaca gctgatgaag cgcagaggag
     4981 gcgtggggca cgacctttcc catatcagac ctacgggtag tgcggttctc aacagcgctc
     5041 tgacttcgac gggtgtggtt ccttttatgg agagatattc caactcaacc cgtgaggtag
     5101 cgcaggatgg tcgcagaggt gcgcttatgc tcaccatttc catcaaacat cccgatgcgg
     5161 agaatttcat tgatgcgaag atggagcagg gcaaggtgac cggagccaat gtctccgtca
     5221 aggtagatga tgattttatg cgtgctgctc tctcgggcag caattatacc cagcagtatc
     5281 ccgtttatag caagaagccg aaggtgactc aggaaattga tgcctcttcg ctctggaaaa
     5341 agattattca caatgcctgg aagagtgcgg aacccggagt cctgttttgg gataccatcc
     5401 taagggagtc cgtggccgat tgctatgcag ataaaggcta caggaccgtc agcacaaatc
     5461 cctgtggaga gattccccta tgtccctacg attcctgccg cctgattgcc atcaacctct
     5521 actcttatgt ggatcatcca ttcacaccca aggcttcatt caacttccct cttttcaagg
     5581 agcattgcgt aaaggcgatg cgcctgatgg atgacctaat tgacctcgag atggaaaaaa
     5641 tcgaccttat catggccaaa atcaaagccg acccggagga tgattatgtc aagaggacag
     5701 agtatgagct ctgggataaa atcagcaaaa aatcgctggg tggacgcagg acgggcctcg
     5761 gcattactgc ggaaggcgac atgctcgcgg ctctgggact gacctacggt accatggagg
     5821 ccatagagtt tgccgttcag gtacagaaaa cacttgccgt agaggcctat aggtcctctg
     5881 taatcatggc ggaggagcgt ggggcattcc ccatatacga ggcatcgaaa gaggtcaaca
     5941 acccaatgat catccgcatc aagaccgagg accccgatct ttatgcccgt atggtaaaat
     6001 cgggtcgccg caacatttcc atgcttacca tcgctcccac cggcactacc agtcttatga
     6061 cacagaccac ttccggtata gaaccggtgt tcagaccctt ctatctccgc agaaaaaagg
     6121 tcaatcccaa cgatgtgaat gtcaaagtct cctatacgga tgacatgggc gacagctttg
     6181 aagagtattt cgtattccat cacaagttcc ttacctgggc catgataaac ggttacgaga
     6241 gggatcaggt attgggaatg tgcgaagatg agctcgaaga cctccttaaa aagtctcctt
     6301 actacaaggc tacggccaat gacattgact gggtatccaa ggtaaagatg cagggtgaaa
     6361 tccaaaaatg ggtggatcac tccataagcg ttaccgtcaa tcttccggaa gatattacgg
     6421 aggagaccgt tgcggaggtt tacaagacgg cctggaattc gggatgcaag ggcataaccg
     6481 tctatcgtga aggcagccgt tccggcgtac ttgtctctac cagcaaggat acacccgtgc
     6541 taaaacccaa aaagcgtcct cagactctgg aggccgacat catccgcttc cgcaacaaat
     6601 cggagaagtg gattgccttt gtaggtaagc tcaacggaca gccctatgag atattcacag
     6661 gtattgtgga cgaagatacc cgcgtaatcc cgaggtctat cgaaaaggga tggattgtca
     6721 aggagaagga tctcaagagc ggcaaatcga gatatgattt tcattatgta gacgctctcg
     6781 gctatccgag cgtactcggc ggtatctccc acatgttcaa taaggagttc tggaactacg
     6841 ccaagctaat ttcaggtgtt atacgtaatg gtatgcccat agtggacatc ctcaacctcg
     6901 tccagggact cgaactggac actgaatcca tcaacacctg gaaaaatgga gtggagaggg
     6961 cactcaagcg ctatattccg gacggtaccc gtgacgaaac aggccaaaag tgcagcaatt
     7021 gtaccagcaa aaaccttgtc tatcaggagg gctgtctggt ctgtctcgaa tgcggctatt
     7081 ccaagtgcag ttagtagaaa aatttttata gagagacagg atgatatttt ttccgaatgc
     7141 gaaaatcaat ctgggtctcg atatcataga gaagcgtccc gacggttttc acaatatcga
     7201 gacgcttttt gtgccgcata cggagtgcta cgacattctt gaggtgctat acgccgaaaa
     7261 attctccatt cacctatacg ggattcctct tgagggtgat ccgatggata acctctgcgt
     7321 caaggccttc cgcctgatgg agaagatata cgacataccg cccgtatcga tccgcctata
     7381 caaaaaaatc ccggtaggtg ccggtcttgg cggcggctct tccgatgctg cgggtacgct
     7441 gatattgctt aacaggatgt actcgctggg tctgagtgac gaagtgctgg ccggtcatgc
     7501 cgcttcactc ggcagtgatt gtcctttctt tatatacaat cgtccgatgt atgcgaccgg
     7561 tcgtggcgag atgctttccc cagtggaact tgctctcgat gattaccgta tcgaactggt
     7621 gactcccgat atattcatct ccacaaaaga ggcctattcc ggagtaactc cgcgcagacc
     7681 tgcggtgccg atttcggaga tcatcaaaaa gcccgtggaa gagtggaagg gatttttaaa
     7741 aaatgatttc gaggaatcgc ttttcctcag atatccaaga cttgcggcgg aaaaagaggc
     7801 tctatatgcc agaggtgcgg tatatgcctc aatgtcgggt tccggcagtt ctctatacgg
     7861 attattcaaa aaatagtgct atcttgtagt ctcaaaatca ggagcaatgc atataggtat
     7921 agcgggaaat attggaagcg gtaagaccac tcttacccgg atgttggcgc agcattatgg
     7981 ctggacgccc aaatatgaag ctgtcaccta caatccctat ctcgaagatt attacaagga
     8041 tattccgcgc tggtcattca atctcgaagt ctattttctt actcaaagaa ttaaggatct
     8101 gatggatatt gccaatagcg acaaggtcat catccaggac aggaccatat ttgaaggagt
     8161 ctatgttttc gtggccaaca ataaggatat gggcaatctt tctcagcggg atttcgatgc
     8221 atacatggac cttttcgggg taatgatgaa gatgatcaag gtgcctgatc tgctcattta
     8281 tcttaggtca tcaattcctc atctggtgag ccggatccag aaacgcggac gtgactacga
     8341 gcagagcatg agcctggagt atcttggcgg cctgaatgag cgctacgaaa aatggatatc
     8401 ggaatatccg ggtgaagtac tcatcatcga tgccgacaat ctcgattttg aaaacaggcc
     8461 ggaggacttt gcttccatta ccaaccgcct cgacgcccgc ctgttcgggc ttttctagaa
     8521 cgggttgcgg ggtacgggtt ccgggttgcg atttttttgt tttatttctg aaatgcatta
     8581 tatttgcagc gtaatagaaa taaaataaga agatgacata tctggaattt agagcgcaac
     8641 tatttgattt ggcatgcttc aatatccatc aggtgtatgc atggcaaccc gattttgacc
     8701 ggaataacct tacacgctgg gtgaaaaaag gttatcttat ccggctgagg cagggatatt
     8761 atgcattttc tgaatataag ggcaagcctg attatcctct ctattttgcc aaccggattt
     8821 accgtccctc ttatattagt ctccacacgg cattatcctt ctacggaatg ataccggaag
     8881 cggtagtgca gatcaccagt gtaacatctt taaagacagc atcattcgcc aatgatttcg
     8941 gcgaatattc ctacaaaaac gtcaaggaaa acctgatgtt cggatatgat ttgaagcaaa
     9001 tggtagacaa ccgaaccata cgctttgcca caccggagaa agctctgctg gatttgctct
     9061 acctatatcc cttctataac acggaacggg agttggagga gttgcgtttg gatgactatt
     9121 tcatgcatga agatttgaat gaggatttac tgatggtata ttgcgaaaag tttcaaagca
     9181 aggcgcttga caatcggata gggttacttt ttaaggctta tgatttatga tacggattga
     9241 acaaatcaga aattttttcc cggctcaaat acgcagagat tcgggatttg ataaatatat
     9301 gctgaaagag tatttgcaat tgatgattct ggattatctt tcatctactc caagcatccg
     9361 aaagatggct tttatcggag gaacgaacct tcgtttggtc aaaggaatag atcgtttttc
     9421 cgaagatttg gatttcgatt gcaaggagtt ctcaaaagag gaatttgatg aaatgacaaa
     9481 cggcgtagtt caatttttgg aacgttcggg ccttcgtgtg gaggtcaggg ataaagagaa
     9541 tccgaaactg acggcattta gaaggaatat ttattttccc gaattgttgt ttgatttggg
     9601 attgagcggt cataaagaag aacgttttat gataaaaatt gaaagccagg atcaggggat
     9661 ctcataccct cacgttatga cgaatatcaa aggttgcgga ttctttttcc cctttcccgt
     9721 tccctccgat ggggttttat gcagcatgaa gatagctgca atgttatctc gtgccaaagg
     9781 acgtgatttt tatgacctga tgtttttact tccacagtca aaaccggatt atgactttct
     9841 ttcaaaacgt tgtggaatac acaatttgca agagttcaaa caagcaacat ctgagctgct
     9901 caaaacagtt gacttgaata aaaagcaaaa agattttgaa cacttgcttt tcaacaaaac
     9961 aagtagtgag aaaattttgc ggtttgggga atttgtggat tctttgtagc gctgtccgta
    10021 tgatgtcaaa aacatatatc catccgaggc ttggtgagat tattataaga aaaagccggc
    10081 tgtcgagaag agttaagctg attgtacacc cgataaacgg gatttcggta acggtgccat
    10141 ggttcgtcag ctactcgaag gcaatcgctt ttgttgagga gaaagaggag tggatagcgg
    10201 ctacgctttt aaaacagagg aaaaagagga aggaggaagc agtcaggatc gctcctgacc
    10261 aaccccttaa attgattacc ggagagattc catacgagag gctcaaaatg gcttcgtcag
    10321 atggcattat atcggaagag gatattgtaa agataatccg cgccgaagca aaaaaatatc
    10381 ttccgcaacg agtggagctg ctcgctgcca aatatggctt taaccccggt aaagtgacgg
    10441 taagaaataa ccgcacaaac tggggcagtt gctcaaaatc gggaaatatc agcctcaaca
    10501 tccaccttat gcgactcact ccggagctcg ccgactttgt gattgttcac gaactttgtc
    10561 atttgattca taaaaatcac gggccccaat ttcacgccct gctcaactct ctttgcaacg
    10621 gcagagagcg tgaattgaac agattgctta aacaacagag acctcaggtg ctatttgtta
    10681 ccgaatagaa tctgtaaatt gcagcgaaaa aggttttgga gctcttacct cattcaaaac
    10741 gtaatcctat aacttattga gggcatgatt gggaataacg aaagttgttt ccaattattg
    10801 ttttcggagt cgtaccagag tgtgaaggca ttgtgtctgt tgaggacgtt gaagataccc
    10861 acattcagat agtgtttgac cttcggtctt tcccatttga agctgtagcc gatatcccat
    10921 cttatgtagg tgggcatccg gtagttgttt ggcctcccga aataatcgag gataatgggg
    10981 gatccgtcag gaagcactcc gggataaaca gaggttttta cgctctccca atggccgctc
    11041 tgcaacgtga aagcactatt gaatccgccg taagaggctg aaaggttcaa tatatggcga
    11101 cggtcgaatt ttgcggggaa actctctcca tagttgatgt tatcgaatgt cctgtcggtt
    11161 ttggagaggg tgtaagcaac tctgatatga gttttagggg cagtatatcg atacatgaac
    11221 tcgcctccgt aggatgttcc ttttcccatc tcgatattgt cgctccatcc ggagagggaa
    11281 gcgctgaaca gagtagtggc atcggtataa aataccagat tattcataaa cttgtagtaa
    11341 ccaccgagtg aaacaaaatg gttacccgga gttgagctca agccgagata gaactgagta
    11401 gactcttctg gcttgttctt tttgccggca ggcaccatca ggtccattga ccatcctatc
    11461 ggcaacccct caagtacgtg ataaaactgc acggtcctgt ctaccgatgc ttcgagttgg
    11521 agatattttg aaaaaatata gcttaaaagg aggcttcctt cgggtctgaa caatacccct
    11581 ctttctgtgt tgagttccga ctggaggtcg ggagcgtata gagatgcttt cagcgatagc
    11641 ttaaaaaaga ggtttcttat ggccgtggtg gagtattgaa aatagagtgt gttcaggatc
    11701 gaatgatggg gatttctcac ataagccttt ttttccacat tacgaccttc gccctcatag
    11761 agttttgacg atccgggggc attcagggag tacatcagct tcaatcctgt aataaaggaa
    11821 tggccgtgcc tttctttttc ggttttcagg ttcaatgtaa tctcattcag accggatctg
    11881 attgccaact cattaaaagt cccttccata tagacttcct gtctctgtcc tgaggaaaag
    11941 cggttgtaag ccaatgaggc atcgtatagt ctgccgctct tcattatccg attgtaggtc
    12001 gccttgctca caatattgcc ccatctgaga taattcttgg acaaatcctc ctttccgaag
    12061 ccgtagctat cttcagttgc aaagagaaag aactccaatc gggaagcgtc atcaatccta
    12121 tatgaaaact ttccgtagag gtcgaaaata ttggtctgca acttatcaaa ggggagtttt
    12181 tctttcggta gaaaggattt caatgccttg tactcgagct ggaaagggga gtatcttcct
    12241 gagaggagta atgatgccct ctcctttgag aggggaatat tggctgaagc tcccgcaaaa
    12301 atatt
//
LOCUS       DAOSIE010000013        11817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_5428_length_11817_cov_3.930114, whole genome shotgun sequence.
ACCESSION   DAOSIE010000013 DAOSIE010000000
VERSION     DAOSIE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11817
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5428_length_11817_cov_3.930114"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..900
                     /locus_tag="PLI69_00975"
     CDS             235..900
                     /locus_tag="PLI69_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOG41097.1"
                     /translation="MANIFTSSIGKKVIMSISGLFLILFLSLHMLLNITYIFSEGTFL
                     QVIDFMGLPIVTIMVPVLAFGFVIHILYAFILTLGNLRARGGYNRYAVPNKAATDSWA
                     SRNMVVLGIIVALGLILHLTHFWVDMQFAEWMGGEVANAPDLMVSTFGNVWVTVLYII
                     WFIAIWFHLTHGFWSAFQSIGLNNTVWFKRLKVVGIVLVTLIMSGFTLIAIYTCCKAN
                     GLL"
     gene            complement(915..2039)
                     /gene="hemW"
                     /locus_tag="PLI69_00980"
     CDS             complement(915..2039)
                     /gene="hemW"
                     /locus_tag="PLI69_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOG41098.1"
                     /translation="MAGIYIHLPFCSSFCLYCDFYSEIDGRARRRRYLKALNSEISER
                     VDFFEGVLPDTIYMGGGTPSLLDTEEIAEFSHTLCNRFNITSLREFTLEANPDDITPP
                     KLEGWLRAGVDRLSIGVQSFCDDHLVWMRRRHSSAQAINAFKMAREAGFNNISLDLIF
                     GFEGLSYEQWEYNIEQLTALYPEHVSCYQMSVESGSELASLVDSGSYHEPSQEICATQ
                     YSVLQQRLEKAGYRQYEISNFALPGWESIHNSSYWERMPYLGLGASAHSFDGEGCRCW
                     NIADINSYVEAIEGNYYDKIKSEEILSDDDKFNEQIMLGLRKVTGLSLSSLESSRLAK
                     IKPVLDYLTSTGDILYEKGIIRIPAGRLFLSDGIMQQLFL"
     gene            complement(2049..2897)
                     /locus_tag="PLI69_00985"
     CDS             complement(2049..2897)
                     /locus_tag="PLI69_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41099.1"
                     /translation="MKKIVITLSVVAAVLLGVLVFIWVSKQKLVKDLNLEKEDLTTEL
                     IALQNDYATLTSTNKAINDSLAVEREKVGQLIERIQKTEATNRSKIRQYEKELGTLRS
                     IMKGYIVQIDSLNSLNVALRQEASEARLEAEESRKQYDELVTTTEALSAKASAGAVVK
                     ARGVVLSAINASNKETDRSSRVEKLRTCLSLIENSIAEKGPKVIYIRVKGPDGILMTG
                     DQQRIFTIDGEEMIYSASREVDYQGDEVEVCIYFASGQGFVKGIYNVEVYSSEAKLGS
                     ADLLLR"
     gene            3022..3519
                     /locus_tag="PLI69_00990"
     CDS             3022..3519
                     /locus_tag="PLI69_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41100.1"
                     /translation="MKKYLIRSIKYLIYCLVFFFIIVGLLFLWQYAKNPDMTCSCMFK
                     EGSMPKLVIFFLLVAGTYPALGFVKRKFHLNGDYPKYAEIIEDAFREAGYKLDKKDDE
                     KVIFRHAKAVTRLTRMYEDAITFDLTQDPIVVDGIRKDVDRILRNVSYRIRREEEDNS
                     SQDAQ"
     gene            3536..3748
                     /locus_tag="PLI69_00995"
     CDS             3536..3748
                     /locus_tag="PLI69_00995"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOG41101.1"
                     /translation="MKEVARYTEPYLAHIARGLLESEGIMAEVLNENSSLPGFCNMYD
                     GAIKLVVRDEDYDLALKVLETDSSSE"
     gene            complement(3700..4278)
                     /locus_tag="PLI69_01000"
     CDS             complement(3700..4278)
                     /locus_tag="PLI69_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOG41102.1"
                     /translation="MIRIGDILVSSEILTEYFSCDYEKCGGICCIVGDSGAPLEEPEA
                     DILKAEYPNYAAWMSIEGRAQVEKKGFFEIDMDGDMVTPLIGDREECVYTRFEGANCF
                     CAIERAFCAGRCDFAKPISCRLYPIRVSQLSSGMTALNLHRWPICDPAFEKGKKEGIP
                     VYKFLRNPLIFRYGEEFYSELESVSRTLSAKS"
     gene            4386..5345
                     /locus_tag="PLI69_01005"
     CDS             4386..5345
                     /locus_tag="PLI69_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG41103.1"
                     /translation="MMKKIFVTIFVALFTMGVSMAQDLGQATELFNNAGMTLQEGNET
                     AALDLFQKAMDMALQLGDEGTAIVTECKTVIPQLMLKLGKDMIREGNIDDAIVKIKKA
                     AEKAAEYENEGVLLEANDLIGQLKINKLMGVANDLLTAREFAEAVEAYKEVIAVDPNN
                     ANAYFRMGQAANAADNTDVAEEAFIKAKELDESIAENVDRQLSTVYLKKAVASQRAKD
                     LKGALENAQKSIEFSDNANAQKIAGLSALGLKQNAVAADALKAYLAMSPDARDRVQIV
                     YQLGTALIAAGQTGEACGYFKEIAQDPKWGEAARYQITVLKCN"
     gene            5336..7165
                     /locus_tag="PLI69_01010"
     CDS             5336..7165
                     /locus_tag="PLI69_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOG41104.1"
                     /translation="MQLELELLAPARNKDIGTAAIDCGADAVYIAGPSFGAREAAGNP
                     VSAIGQLADYAHKYSAKVYAVFNTILFDDELEAAGKLLWELYHAGVDAFIVQDLAIAR
                     MEKPPLPLFASTQTHIRTPQQAQFLSSLGFKRLILERQLSLAQIKEIRRSTTCDLEFF
                     VHGALCLSYSGQCYLSQRLAGRSANRGACIQACRSDYDLVDSNGKVLVKNRPLLSLKD
                     LNLSEYIGDLIEAGITSFKIEGRLKNASYVKNVVREYRRIIDRFISSHPNYGKASAGE
                     IYGGFTPDVNATFNRGYTSCFIEGKRGLWASSDGTKSMGEYLGEIFSVKGDWISISTD
                     KELNNGDGLAVISPSGEIKGFRADVCEGNRVRIKEASRLRKGMKVYRNLNTAFEKELE
                     KNMPERWIKATISCTSKAGVTTFEAVSSQDRAILTFDDDNPPAQKPEKALEALSRQLS
                     KSSGEYIFTLGDVECDIIRFYPASALNGIRRQLAELLKEAALSRMAEGRNIDPDEMVA
                     RNVAKYPERHLTYLANCSNQLSRDLYLESGVKSVGPAYEIEAPEEVAVMRTKHCIKYE
                     LGLCHKNPEESVADFREPLYLANRNYKLRLEFDCKNCEMIVNL"
     gene            7191..>7854
                     /locus_tag="PLI69_01015"
     CDS             7191..>7854
                     /locus_tag="PLI69_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOG41105.1"
                     /translation="MRFADVIGDKGLKERLIRMVEHKRLSHAILFREEPNGGGLAFAL
                     ALGQYVNCRHRSDGDSCGICPTCNQFQKLIHPDLHFAFPVNSSREVAESQKGRPISDH
                     FLDNWRELVLNDPYFTEQDLYDAIGIDNKSGNISVHEARRIIERLQLRSFEADYKVMV
                     IWLPEKMNQECSNKLLKLLEEPPTGTLFLLVSQAPEKLLATIRSRCQLIEIPPMSAEQ
                     HQS"
     assembly_gap    7855..7864
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7865..8237
                     /locus_tag="PLI69_01020"
     CDS             <7865..8237
                     /locus_tag="PLI69_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41106.1"
                     /translation="LQEPVEYRDMLTGLIEAGISKKLIDTFAIWEALSEMGREKQKEF
                     CIYSEKFIRKIYMTSQGLEQIAGIFDQEESQIRDLASRIKPDFYEKALSAFDRTISAI
                     ESNTNSKLTFCDLSNRLLLYL"
     gene            8240..9571
                     /gene="ricT"
                     /locus_tag="PLI69_01025"
     CDS             8240..9571
                     /gene="ricT"
                     /locus_tag="PLI69_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOG41107.1"
                     /translation="MEEKDQKKIYDCSRGCEVERTPHGALSISYSPGCCKLADFDWMQ
                     GISQERYKDLFEVRFKNTRKIVYKNTTGQLIKIGDLVVVEAPTGHDVGIVTLEGPVVG
                     RQLLRHNIDPSTYEFRRIYRRAKILDIERWQEAIAREHKTMIRARQIAAGLGLDMKIG
                     DVEFQGDGTKAIFYYIADERVDFRQLIKDFAEEFHIRIEMKQIGARQEAGLIGGIGVC
                     GRALCCSAYMSDFKSITTQAARCQDLSLNPQKLAGQCSKLKCCINYEASLYIDAQKHL
                     PDVRGPLEFKDGSAYLQKCDILKGIMYFSYDPHCMINLFPLSGQRVREIISMNRKGIK
                     PDSLKDKETDVRETDFISAVGDDSISRFDEKRRSKRKKEFKRPERNRKQQFEDDKNRQ
                     EGDAKPRFDGKPQSGQNKGYRRQGQRSGIKVRDQEKQGRKGEENREPSDNQ"
     gene            9537..10031
                     /locus_tag="PLI69_01030"
     CDS             9537..10031
                     /locus_tag="PLI69_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41108.1"
                     /translation="MKKIESHPIISRMFAIMLTLSAVCTIILGSCSRPDDGYRYIKVD
                     PDCDSIPVFRFSLPVADSTALYSTFIAARYDCRLHSNLISFNVEVTSPDGEIFTEKIT
                     LPLTDNPQVEIRRSRFGIVDIKWPYRDNIKACEGEWSVALTPLHKDVRKWLHGVGFSY
                     HINE"
     gene            10045..10794
                     /gene="trmB"
                     /locus_tag="PLI69_01035"
     CDS             10045..10794
                     /gene="trmB"
                     /locus_tag="PLI69_01035"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOG41109.1"
                     /translation="MSKKNKLLKFRENETFKCLIQPRTEEVFRCDHPRKGSWHKAIFG
                     NDNPIVLELGCGKGEYTIELAERYPERNFIGMDIKGARLWKGAKYANEHKLQNVAFLR
                     TRIEFIESLFAAGEVAEIWITFADPQEGKPKKRLTHPLFLTRYRNILTEGGIVHLKTD
                     SRLLYHYTMEVASANSLEVITHCEDIYGSPESVPEEITAIQTFYESHFLMQGIPITYM
                     AFRINNEEELISPEWDQTPYLQPYKTTTIMK"
     gene            complement(10778..11791)
                     /locus_tag="PLI69_01040"
     CDS             complement(10778..11791)
                     /locus_tag="PLI69_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HOG41110.1"
                     /translation="MKCNKKVFTPEGGGAELQIMVSAPSVESAFKAAADSIPHGFNLI
                     AGRLFLDEFTVVPCMEQPFDSISVVRETPLGDLPVSLWIWASAPSQASGLLVWSANLV
                     SDKESVFDQTNDILCKYQQFLTSQGMNITDNCVRTWFFVDDIDYKYADFVRARRDFFD
                     SVGLRSDTNYIASTGIFGIPGNRPATGGDVMLDALALKYPKKCLIRYLHARSHLSPTS
                     LYGVTFERGTVVDYGDRSHIFISGTASIDSRGEVLHAGDVGKQCDRMMENVSVLLSEA
                     DASMEDVQMAIVYLRNSEDLSMVKQRLSEILRDIPHNYLLAPVCRPEWLVEIECIAAT
                     SLS"
BASE COUNT         3424 a         2692 c         2680 g         3011 t
ORIGIN      
        1 ccacgaacca cggaagctat ggccccggca taacgagcca tgacatcctc atcaagtccc
       61 acaccgtcgg gacctcctat ggcctctccg ctaagcttca gtaatatcct gttgtacttc
      121 atacaagtct attgtttggt tgttacaact aacaaaagta ggtaaatatt atgattttta
      181 caactaaagg tctatatttg cgctctcaaa taacgaagac acaaaatttg acttatggca
      241 aatatattca cttcatccat cgggaaaaag gtaataatga gcatcagcgg attgttctta
      301 atactgtttc tttcactcca catgctccta aatatcacat atattttcag cgaggggaca
      361 ttcctgcaag tcattgactt tatgggtctc cccatcgtga caattatggt gccggtactc
      421 gctttcggat ttgtaataca catcctgtac gcctttattc tcactctcgg caacctgaga
      481 gctcgtggcg gctacaaccg ttacgcagtt cccaataaag cggctaccga ttcatgggct
      541 tcacgcaaca tggtggttct cggcataata gtggcccttg gccttatact gcatctgaca
      601 catttctggg tggacatgca gtttgcagag tggatgggag gggaggtagc caacgccccc
      661 gatctaatgg tctcgacctt tggaaatgta tgggtaaccg ttttatacat tatttggttt
      721 atagccatct ggtttcacct tacccacggt ttttggagtg cctttcagtc tatcgggctg
      781 aataacaccg tctggttcaa gagattgaaa gtggtgggga ttgtgttggt aaccctgata
      841 atgtcaggtt ttaccctgat agccatttat acctgctgca aagcaaacgg cctgttatag
      901 gttataggaa gtatttaaag gaaaagttgt tgcattatcc cgtccgagag gaacaatctc
      961 cctgccggga tccgtataat gcccttttca taaagtatat cgccggtgct cgtcaaataa
     1021 tcgagtaccg gttttatttt tgccagtcgt gaactctcaa gggaagagag agataaaccg
     1081 gtaacttttc gtagtccaag cataatctgc tcgttgaatt tgtcatcgtc ggataggatt
     1141 tcctcgctct ttattttgtc gtaataattc ccttcaatag cctctacata actattaata
     1201 tctgcaatat tccagcagcg gcagccctca ccatcaaaag agtgtgccga agctcccaaa
     1261 ccgagatagg gcattctctc ccaataagag gagttgtgga tggattccca accgggaagg
     1321 gcaaaattag agatctcgta ctggcggtaa ccggcctttt ccagcctctg ctgtaaaaca
     1381 gaatattgtg ttgcacaaat ctcttgcgaa ggctcatggt agctgcccga gtcgaccagc
     1441 gaagcgagtt cgctaccgga ttctacgctc atctggtaac aggaaacatg ctcgggataa
     1501 agagccgtaa gctgctctat gttgtactcc cattgctcgt aggagagtcc ttcaaaacca
     1561 aatatcaaat ccagtgaaat attgttaaaa ccggcctccc gtgccatctt gaaggcattt
     1621 attgcctggg ctgaggaatg cctgcggcgc atccatacga gatggtcatc acagaatgac
     1681 tgaactccaa tactcaacct gtctactcct gcacggagcc acccctctag tttcggtgga
     1741 gttatgtcat cgggattggc ctccaatgta aattcacgaa gagatgttat attaaatcta
     1801 ttgcacagag tatgagagaa ctccgctatc tcttcggtat ccaaaagcga aggagtgccc
     1861 ccacccatgt aaatggtatc ggggagcact ccttcaaaaa aatcaactct ttccgaaatc
     1921 tcggaattaa gggctttcaa gtagcgtctg cgccgcgctc tgccatcaat ctccgaataa
     1981 aagtcgcaat aaaggcaaaa agaggaacaa aaaggcagat ggatgtagat acccgccata
     2041 acaggtctct acctcaggag tagatccgca ctgccgagtt tggcctctga agagtaaacc
     2101 tcaacattgt agatgccctt cacaaagccc tgaccactgg cgaagtagat acaaacctcc
     2161 acctcatcgc cctggtaatc aacctcacgt gaagcggaat agatcatctc ctcgccatca
     2221 atggtaaaga tcctctgctg atcgcctgtc ataaggatgc catcgggacc cttaactctg
     2281 atatagataa cctttggacc cttctctgca atggagttct cgatcagact gaggcaggta
     2341 cgtagtttct ccacacggct gctgcggtca gtctccttgt tggaagcatt tatggccgag
     2401 agtactactc ccctggcttt cactactgcg ccggcggatg cctttgcaga caatgcctca
     2461 gttgtggtta ccagctcatc atattgctta cggctctcct cggcctccaa gcgggcttcc
     2521 gacgcctcct ggcgcagagc aacatttaga ctgttaagag aatctatctg gacaatatag
     2581 cctttcatga tggaacgaag cgtgccaagc tccttttcgt actgacgaat cttagacctg
     2641 ttggtagcct ctgtcttctg aatacgctcg attagttggc caaccttctc cctctctact
     2701 gctagagagt cgttgatcgc cttgttggtc gatgtaagag tggcataatc attctgtagg
     2761 gcaatcagtt cggtggtaag gtcttctttt tcaagattaa ggtccttaac caatttctgt
     2821 ttgctaaccc aaataaatac cagcactccc aaaaggactg ctgccacaac ggataatgta
     2881 attacaattt ttttcatcgt atgtatgact gttttgtgtt tttcttaatt attctacaaa
     2941 aataattcct ttttttgaaa cataacatta aatttgcaga aaatagttaa aacgactcat
     3001 cattaacaca ataacaacgc aatgaagaag tatttgatac gctcgataaa atacctgatc
     3061 tattgtctgg ttttcttttt tatcatagtt gggttgcttt tcctatggca atatgccaag
     3121 aatcccgaca tgacctgttc ttgtatgttc aaggagggtt caatgcccaa actggttatt
     3181 ttctttttac tggttgccgg aacatatccg gctttgggat ttgtcaagag aaaatttcat
     3241 ctgaatggtg attacccgaa atatgcggag ataattgagg atgccttcag ggaagcagga
     3301 tataaactgg ataagaaaga tgatgaaaag gtgattttca ggcatgccaa agcggttaca
     3361 cgcctgacaa ggatgtacga ggatgccatc acgttcgatc ttacacaaga tcccattgta
     3421 gtggatggta ttcgcaagga tgtggaccgc atcctacgca acgtcagtta tcgtataaga
     3481 cgcgaagaag aggacaattc ctcacaggat gcacaataaa ataacaaatt gtaaaatgaa
     3541 agaagttgca aggtatactg agccatatct ggcccacatt gccagagggt tacttgaaag
     3601 cgaaggcatt atggccgagg ttctcaatga aaactcatcc ctgcccggtt tctgtaatat
     3661 gtatgacgga gccatcaaac tggtggtgag ggacgaggac tacgatttgg cactcaaggt
     3721 tctggaaact gactccagtt ccgagtaaaa ttcctcgcca tacctgaata ttaagggatt
     3781 cctgagaaat ttgtagacgg ggatcccttc ttttttgcct ttctcgaaag cggggtcaca
     3841 gataggccaa cgatgtaaat tgagagcagt cattccactc gaaagttgag ataccctgat
     3901 cggatagagg cgacaggata taggctttgc aaaatcgcac ctgccggcac aaaatgccct
     3961 ctctatggca cagaagcagt tagctccctc gaatcgggtg tatacacact cctcccggtc
     4021 accaatgagc ggagtcacca tatcgccatc catatcaatc tcgaaaaaac ccttcttttc
     4081 aacctgcgca cgaccctcta tactcatcca ggctgcatag tttggatatt ccgcttttaa
     4141 aatatccgct tccggctcct ccagaggtgc accgctatca cccactatac aacaaatacc
     4201 accgcatttt tcataatcac aactaaaata ttcggtaagt atttctgatg acacaagtat
     4261 atctccgata cgtataatcg aggtgtgcat ttcttttttt tgcaaaaata aagataagtt
     4321 tctaattttg gccggattta tgcaataagc atttgtaata caaattttaa ttacaagaac
     4381 aagatatgat gaaaaagatt tttgttacca tttttgttgc cctctttacc atgggcgttt
     4441 ctatggctca ggacctcggt caggcaacgg aacttttcaa caacgcaggt atgacacttc
     4501 aggaaggcaa tgagactgcc gctctggatc ttttccagaa ggctatggat atggccttac
     4561 agctaggtga tgagggtact gcaatagtta ccgagtgtaa aacggttatt ccccaactta
     4621 tgcttaagct aggtaaggat atgataaggg agggtaatat tgacgacgct atagtaaaga
     4681 tcaaaaaggc ggcagaaaaa gcagcggaat atgagaacga aggcgtacta ctcgaggcca
     4741 acgaccttat cggccaactc aagataaata aacttatggg ggttgccaat gacctgctta
     4801 cagccagaga gtttgccgaa gccgttgaag catacaaaga ggttatagca gtagacccca
     4861 ataatgccaa cgcctatttc cgtatgggac aggctgcaaa tgcagcagac aataccgatg
     4921 ttgcagaaga ggccttcatc aaggccaaag aacttgatga gagcattgca gaaaatgttg
     4981 acagacagct ttcaaccgta tatctaaaga aggcagttgc aagtcagaga gccaaggacc
     5041 tcaaaggcgc tcttgaaaat gcacagaaat ccatagagtt cagcgataat gccaacgctc
     5101 agaagatagc gggcctgtcg gctcttggtc tgaaacaaaa tgcagtagct gcagatgcac
     5161 ttaaggctta tctcgcaatg agtccggatg caagagacag agtgcagatc gtataccagt
     5221 tgggtacagc cctgattgct gcaggccaga ccggcgaagc ttgcggttac tttaaagaga
     5281 tcgcacagga tcctaagtgg ggtgaagctg ccagatatca gataaccgtt cttaaatgca
     5341 actagagctc gagcttctcg ctccggcgcg taataaagac atcggcactg cagcgataga
     5401 ctgcggtgcc gatgccgtat atattgcagg cccctctttt ggggcgagag aggctgcagg
     5461 caatcccgtt tcggcaatag gacaacttgc ggattatgct cataaatact ctgcgaaagt
     5521 ctatgcggta ttcaatacca tcctatttga cgatgagctg gaggctgccg gtaaattact
     5581 atgggaactt tatcatgccg gagtggatgc cttcattgtc caagacctgg ccattgcccg
     5641 aatggagaaa cctcccctac ctctttttgc ctctacccaa acccatattc ggactccgca
     5701 gcaggctcaa tttctctcct ctctcggctt caaacggttg attttggagc gtcagctctc
     5761 actcgcacaa attaaggaaa tcagacgcag taccacttgt gacctggaat tcttcgtaca
     5821 cggggcactt tgcctctcct acagcggaca gtgctattta agtcagcgtc ttgccggccg
     5881 tagcgctaac agaggagcct gtattcaagc ctgcagatcc gactatgatc tggtagacag
     5941 caatggtaag gtgcttgtta aaaacaggcc gttactttct ctaaaggatt tgaatctgag
     6001 tgagtatatc ggggatttga tagaggcggg catcacctcc ttcaaaattg aaggaagact
     6061 caaaaatgct tcctacgtta agaatgttgt aagggaatat cgccgcatca tagatcggtt
     6121 tataagcagc catccaaatt atgggaaggc ctctgccggc gagatatatg gaggctttac
     6181 ccccgatgtg aatgccactt tcaatcgagg ctacacatcc tgcttcatag agggtaaaag
     6241 agggttgtgg gcctcttctg acgggactaa atccatgggt gagtatcttg gagagatttt
     6301 ctccgtcaag ggagattgga tatcaatctc caccgataag gagctcaaca atggtgacgg
     6361 tctggctgtc atttctcctt ccggggaaat taaaggattc agggctgacg tatgcgaagg
     6421 caaccgtgta cgcattaagg aggcatccag actacgcaaa ggtatgaagg tctaccgcaa
     6481 tctcaacacc gcttttgaaa aagagctgga aaaaaatatg cctgaaagat ggattaaagc
     6541 cactattagt tgtacttcta aagccggagt caccacattc gaggctgtca gctctcagga
     6601 cagggcaatc ctcacttttg atgacgacaa tcctccggct caaaaacctg aaaaggcttt
     6661 ggaagctctt tccaggcagt taagtaaaag ttcaggagaa tatattttca cgcttggcga
     6721 tgtagagtgc gacataataa ggttttatcc tgcctctgcc ctcaacggca tcagacgcca
     6781 acttgcagag ttgctcaaag aggctgcttt gagccgaatg gctgaaggca gaaatattga
     6841 ccccgatgag atggttgctc ggaatgtggc caaatatccc gaaaggcatc ttacctatct
     6901 ggccaactgc tccaaccaac taagcagaga cctctatttg gagagtgggg taaaatctgt
     6961 aggccccgct tatgaaatag aagctcctga agaggtagct gtgatgcgga ctaaacattg
     7021 catcaaatat gaactcggcc tctgccacaa aaatccggaa gaaagtgtgg ctgatttcag
     7081 ggagcccctc tacctggcta acaggaatta caaattgaga ttggaatttg attgcaaaaa
     7141 ttgcgaaatg attgtaaatt tgtagtttag accaacaatc tttgtctagg atgagatttg
     7201 cagatgtcat aggagataag ggcctcaagg agcgacttat aaggatggtt gagcataaac
     7261 ggctctccca cgccatcctt ttcagagaag aacctaacgg tggaggtctc gcttttgcct
     7321 tggcgctagg ccagtatgtc aattgtcgcc accgtagtga tggcgactcc tgtggtatct
     7381 gccctacctg caatcagttt cagaagctta tccatcccga tctgcatttt gcatttcccg
     7441 tcaatagttc cagagaggta gcggaaagtc aaaagggacg accgatttcc gaccattttc
     7501 ttgacaactg gagggaattg gtgttgaatg acccttattt tacagaacaa gacctctacg
     7561 atgctatcgg tattgacaac aagagtggca acatcagtgt tcacgaagcg agacgcatca
     7621 ttgagaggct acagttgcgt tcttttgaag ccgattacaa agtaatggtg atatggcttc
     7681 cggaaaagat gaaccaggag tgttcaaaca aactcctgaa acttctggaa gagcctccga
     7741 caggcacact attcctgttg gtatcacagg cccccgaaaa gcttctcgcc accatccgtt
     7801 caagatgcca gcttatagaa atacctccta tgagtgccga acagcatcaa agcannnnnn
     7861 nnnnattaca ggagccggtc gaataccgtg acatgcttac cggactgatt gaagccggta
     7921 tctccaaaaa gctgatcgat acctttgcca tctgggaggc actctcagag atgggaaggg
     7981 agaagcagaa agaattctgt atttatagtg aaaaatttat cagaaaaata tatatgacct
     8041 cgcagggtct ggaacaaata gctggcatat ttgatcaaga ggagagtcaa atccgcgatc
     8101 tggcctcacg tataaaaccc gatttttacg aaaaggctct gtcggctttt gacagaacta
     8161 tatcagctat cgaaagtaac accaactcca aacttacatt ctgcgacttg tcaaaccgtt
     8221 tgttactcta cttgtaatta tggaagaaaa agaccaaaag aaaatatatg attgctcaag
     8281 aggttgtgaa gtcgaaagga ctccacacgg agcactctcc atatcctata gtcccggctg
     8341 ctgcaaactt gcagacttcg actggatgca gggtatatct caggagcgct ataaagactt
     8401 atttgaggta agattcaaaa atacccgtaa aatcgtctat aaaaacacta ccggacaact
     8461 tattaaaata ggagatttgg tggtggtgga ggctcccacc ggtcacgatg taggtattgt
     8521 aactcttgaa ggtcccgtag tgggacgtca actgttgcga cacaatatcg atccatcaac
     8581 gtacgaattc agacgtatct accgtagagc gaagattctt gacatagaaa gatggcaaga
     8641 ggccattgcc cgcgaacaca agacgatgat acgtgctcgc cagattgctg caggacttgg
     8701 acttgatatg aaaataggcg atgtagaatt tcagggggac ggtacaaaag ccatctttta
     8761 ctatattgcc gatgaaaggg tggatttcag gcaactgatc aaggattttg ccgaggagtt
     8821 ccacatcagg atagagatga aacagattgg ggctcgtcag gaagccgggc taatcggagg
     8881 tattggcgtg tgcggcagag ctctctgttg ctcggcatat atgtccgact tcaaatcaat
     8941 caccacccag gccgcacgct gtcaagacct ctcgctcaat cctcagaaac ttgccggaca
     9001 gtgcagtaaa ctcaaatgct gcatcaacta cgaagcctca ctctatatcg atgctcaaaa
     9061 gcaccttccg gatgtacgag ggccgctgga atttaaagat gggagtgcat atcttcagaa
     9121 gtgtgacata ctaaaaggaa tcatgtactt ctcatacgat ccacattgca tgataaatct
     9181 ctttcctcta agcggacaac gcgtaaggga gatcatatct atgaaccgta agggcatcaa
     9241 acccgattcc cttaaagata aagaaacgga tgtgcgcgaa acagacttca taagtgccgt
     9301 aggtgacgac agtatctctc gttttgacga gaaacgccgt tctaaacgga agaaagagtt
     9361 caaacgcccc gaacggaaca ggaaacaaca attcgaagat gataaaaacc gtcaggaagg
     9421 tgatgccaag cctcgcttcg atggtaaacc tcaaagcggt cagaataaag gctaccgtcg
     9481 tcagggacaa cgtagcggaa taaaagtaag agatcaggaa aaacagggaa gaaaaggtga
     9541 agaaaataga gagccatccg ataatcagta ggatgtttgc cattatgctg acattatcgg
     9601 ccgtttgcac aataatctta ggatcatgct caaggccgga tgacggttat aggtatataa
     9661 aagtagatcc cgactgcgac tccataccgg ttttcagatt ttctctaccg gtggctgaca
     9721 gcacagccct ctactctacc ttcattgcag cacgatatga ttgccggtta cacagtaacc
     9781 tcatatcatt caatgtggag gtgacttcac cggatggtga aatttttacc gaaaaaataa
     9841 ctctacctct tactgataac cctcaggtgg agatcagaag gagtagattc ggtatcgtag
     9901 atatcaagtg gccatacagg gataatataa aagcctgcga aggagagtgg agcgtagctc
     9961 ttacgcctct ccacaaggat gtcaggaaat ggttgcacgg agtaggtttt tcataccata
    10021 tcaacgaatg aatcggcact caacatgagc aaaaaaaata aattgctgaa attcagggaa
    10081 aatgaaactt tcaaatgcct tattcagcca cgtacggaag aggtattccg gtgtgaccat
    10141 ccacgtaagg gaagctggca taaggcgata ttcggcaatg acaatcctat agttttagaa
    10201 ctgggttgcg gtaaaggtga atataccata gagctggctg aaaggtatcc ggagcgcaat
    10261 ttcataggaa tggatataaa aggtgcacga ttatggaaag gagccaaata tgccaatgag
    10321 cataagcttc aaaatgtagc attcctcaga acaaggattg agtttatcga atctcttttc
    10381 gctgccggcg aagtggctga aatatggatt acatttgccg accctcagga gggcaaaccg
    10441 aagaaacgac tcacccatcc tctttttctc accagatatc gcaatatact aacggaaggg
    10501 gggatagtcc atctcaaaac agacagcaga ttgctctatc actatacaat ggaggtggcg
    10561 tcagccaatt cccttgaagt tataactcat tgtgaagata tttatggctc gcctgagtcc
    10621 gttccggaag agataacagc gattcagacc ttttatgaat cacatttttt gatgcagggc
    10681 atccctatca cctatatggc attcaggata aacaatgagg aagaacttat ttcccccgaa
    10741 tgggaccaaa caccctatct ccagccctac aagacaacta cgataatgaa gtagcagcta
    10801 tgcactctat ctccacaagc cattcggggc ggcacacggg agctaaaaga taattgtgag
    10861 gtatatcacg gagaatctcc gaaagtcgct gtttaaccat agagagatcc tcactatttc
    10921 tgagatagac tatagccatc tgcacatctt ccatggaggc atctgcctct gagagcagga
    10981 cagagacatt ttccatcatt ctatcacatt gtttgccgac atcgccggca tggagtacct
    11041 cgccacgaga atcgatgctt gccgtacctg atataaagat atggctgcga tcaccataat
    11101 ccactaccgt acccctttca aaagtgactc cgtacaatga agtaggagag agatgcgagc
    11161 gggcgtgcag atatcttatt aaacactttt tgggatattt gagtgcaaga gcatccagca
    11221 ttacatcgcc tcccgtagcc ggtctgttac cgggaatccc aaaaattccc gtacttgcta
    11281 tatagttggt atcgctcctc aggcctaccg agtcaaaaaa gtctcttctt gctctgacaa
    11341 aatcggcata tttgtagtcg atatcatcga caaaaaacca ggttcgtacg cagttatcgg
    11401 ttatattcat cccttgagag gtgagaaact gctgatactt gcacaagata tcattggtct
    11461 gatcaaaaac agactctttg tcagagacaa gatttgcgct ccatactaat aaaccggaag
    11521 cctgcgatgg ggcggaagcc catatccaga gggagacggg cagatccccc agaggagtct
    11581 ctcttactac tgaaatagaa tcaaaaggct gttccataca gggtactacc gtaaattcat
    11641 ccagaaacag acgacctgca attagattaa atccatgagg aatagagtca gcggcggcct
    11701 taaacgcact ttccaccgaa ggagcactaa ccattatctg tagttctgct ccaccgccct
    11761 caggggtgaa aactttttta ttgcacttca ctctcatagc caaatatgct caacgag
//
LOCUS       DAOSIE010000014        11677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_5495_length_11677_cov_3.998881, whole genome shotgun sequence.
ACCESSION   DAOSIE010000014 DAOSIE010000000
VERSION     DAOSIE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5495_length_11677_cov_3.998881"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLI69_01045"
     CDS             complement(<1..601)
                     /locus_tag="PLI69_01045"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOG41111.1"
                     /translation="MDYNTQRDILHLPEYGRHVQQMIEQVKRIPDREKRNEQIRAVIQ
                     VMAILNPHMKDFPDYKQKLWDHMYIIADFDIDVDSPYPKPDKKEFFSRPEKIQLEEKP
                     MKASCYGRNIENMINLIAAREDDDAKTEMICTLGHYMRQQYLIWNKDSVADETIFSDM
                     EKLSEGRLKVPEGLQLSALSSDATFNRPGVMAPGTSKSGF"
     gene            769..1662
                     /gene="rfbD"
                     /locus_tag="PLI69_01050"
     CDS             769..1662
                     /gene="rfbD"
                     /locus_tag="PLI69_01050"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOG41112.1"
                     /translation="MGKTLVTGSLGQLGSELRELVGNDRNWIFTDALPNEETLQLDIC
                     DSRRVRELMEGQDVDLIINCAAYTNVDKAESEKELSFKINAEGPKVLAECVRERNAAL
                     VHISTDYVFDGKRKRGAYRESDPCRPQSVYGATKRQGELAIRRSRCRGVIIRTAWLYS
                     SYGNNFVKTMVSLGSERSEIGVVADQIGTPTYARDLAKAILTIIPQIGEHRGDIYHYT
                     NEGSCTWFDFAALIMTYCGLDCKVNPLTTEEYPTAARRPAYSVLDKSKIREEFGVETP
                     WWSISLREALRKMGKPVPQHL"
     gene            complement(1625..2164)
                     /gene="rfbC"
                     /locus_tag="PLI69_01055"
     CDS             complement(1625..2164)
                     /gene="rfbC"
                     /locus_tag="PLI69_01055"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOG41113.1"
                     /translation="MNFIKTGIEGPVIIEPKVFYDHRGYFFESFSLREFEANVQKINF
                     VQDNESLSAKRGVIRALHFQKGEAAQAKLIRVVQGRVLDVAVDIRPESPTYGKYVSVE
                     LSAENKRMFFIPRGFAHGYLVLEDNTIFQYKCDNYYAPEMEDTYRWNDPALAIDWGID
                     PKEVILSARDAEAPAFPSS"
     gene            2430..3437
                     /locus_tag="PLI69_01060"
     CDS             2430..3437
                     /locus_tag="PLI69_01060"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOG41114.1"
                     /translation="MNWDKLAFDYVKCNTMVCSFNKGDGWSPLESRTNDKYEITLMSG
                     SLHYAIEIFEGLKAYNGRDGKIRIFRPGENAVRMIRSAEFLGMPPVPEEMFTEAVTRA
                     VAENREFIPPYESGASMYIRPFMISTNPQLTLELGKEFMFVVAVTPTGNFTGTSFNPT
                     KALLVRDHDRAAPLGTGSYKIGANYAASMLAGVQAKKEGYPSILYLDSKEKKYIDEFS
                     SSNFFAIRDNSYITPDSPSILPSITNKSLREIASQMGMTVEHRPVPVEELATFDEAGA
                     CGTALVITPVHEIYDRERDIRYNIGSPVRVGDKSRGLYKHLTGIQFGEIEDSFGWCRI
                     L"
     gene            3623..3952
                     /locus_tag="PLI69_01065"
     CDS             3623..3952
                     /locus_tag="PLI69_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41115.1"
                     /translation="MKSKLFIIALLAIVAVGCERVIDLPEPNVQGGDVVTIEATIPPE
                     TRVAYTDSYTSGNGGTLAWQTGDQLRLAAYNGNTYIGSSLFTFVTGSANKFTGTLVEG
                     ATPHHRL"
     gene            3981..5888
                     /locus_tag="PLI69_01070"
     CDS             3981..5888
                     /locus_tag="PLI69_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOG41116.1"
                     /translation="MDDNGNVQPLAAAFWQQTQVGNGNDHLRDKLILSDVIANTINQS
                     FELLLNSSIVRLELYNMPQDIYELKSVIWTVKTTSGETRSAILDVTGVTAPPTHPYPI
                     PFNLKAYLAFDPDFMQVAVGGKVKITLIGDKSYEWSTTSQDGMTYTKGTRYYATVSGN
                     WMQKVPFIFTIKTNQSNQILTLYQRAASNSPANLTIDWGDGSGYRTFNQGDPIGETID
                     THNYVNAGDYTVTIYSNNPNYSSKQIPQITFYKYSSVNQLLTAVVTPFPNMGADATSF
                     EECFYQCTQLTTIPADLFRYNTQATSFRKCFIFCNGLTSIPSGLFSYNTAATNFNSCF
                     YGCTGLSSIPAELFNNNTQTIDFRSCFANCTGLTAIPANLFTSNDQAQIFENCFNGCT
                     GLTAIPMGLFDNNLAATSFHGCFAGCTGLTAIPENLFQYNTQAKHFLWSFGGCTGLTA
                     IPAGLFSTNTNAINFLGCFINCAGLTAVPADLFRYNVEATNFSNCFYNCTGLTAVPAD
                     LFTYNVEATNFSYCFYNCSGLTALPSHLFRYNTKATDFSYCFSGCIKLQLNLDIFPSP
                     APIFGKPDFFNDRAMDFTDFCKNVGSHPTAISGTAPKLWSFNYGAGVTTTGCFTGANV
                     TNAGDIPNNWK"
     gene            complement(5942..6796)
                     /locus_tag="PLI69_01075"
     CDS             complement(5942..6796)
                     /locus_tag="PLI69_01075"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOG41117.1"
                     /translation="MINEKESVNIFHQPLIEHFERYALIGGMPEVVARYAADRDIVSL
                     GKTYNELLNAYKNDVEKYAETNTQAAVLRYILEEGWAFSGQTITFGGFAESPYKARET
                     GEAFRTLSKAMLLELVYPTTNTIMPATSDLKKTPKLFWLDAGLVNYAAGIQREYLLQK
                     DLMDTWRGAAAEQIAAQELKALSNDVGFRRNYWRRNKRGSTSEVDFIWIQDGLIIPVE
                     VKSGHNAHLKSIHQFMDETNHNVAVRIWSGKYGVDDVKTNAGKSFRLINLPFYLISKL
                     ADIVRETL"
     gene            complement(6817..7278)
                     /locus_tag="PLI69_01080"
     CDS             complement(6817..7278)
                     /locus_tag="PLI69_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG41118.1"
                     /translation="MFKRNIETQLHKWAKNPYRKPLILRGARQVGKTTVVNQFAENFE
                     NFISINLEKSAAKNLMESVDEVKKLIPLLFLYANKTQKEGRTLLFIDEIQNSPHTVSL
                     LRYFYEETPEIYIIAAGSLLETMLDKHISLPVGRVEYMAFILALFPNIWLP"
     gene            complement(7432..8634)
                     /locus_tag="PLI69_01085"
     CDS             complement(7432..8634)
                     /locus_tag="PLI69_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG41119.1"
                     /translation="MKGFFNMAVDSLLDNRLRSTLTILIIATGITSLVSIQTAIEGLS
                     QTVEKSFGRMGAHCFTISSLEEEHEHFARAISWSDVRQFVRRYDSKAVVSINSSIIMQ
                     QVSAGSVISDPTVTVTATDEHHLRLGLASLASGRNFSSREIEGSSDLCIIGDNIARRL
                     FSGNDPLGQTLRVGAVEYLVTGVMEREGALFGIGTDNTVLIPLGPARTRLLSSDAGYS
                     LDIMPVGGESAFEQAWERAVVVMKLIRGLRPQDDANFAISRSDSIMRELDALKGKLSI
                     AALVIGLITLLGASVGLMNILLVSVKERTCEIGIRKALGAKPLTIAMQFLAEAVLVGQ
                     IGCALGLFMGLLFGNLVAKLLGCAFALPWRWLGLSVLICIVVSILSGYLPARRASLLD
                     PIEALRWE"
     gene            8723..>9078
                     /locus_tag="PLI69_01090"
     CDS             8723..>9078
                     /locus_tag="PLI69_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41120.1"
                     /translation="MENRKTERSRKESKGEAYGNKPARKSGVTEKATRRTTRSADRPK
                     RDDDEKFYHKDGGKPTGKGRATVNKKGAVTSRHDREEWHDKPDRRERSERHDRYGKPE
                     SRDRRERSERHDRYDKP"
     assembly_gap    9079..9178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9179..10055
                     /locus_tag="PLI69_01095"
     CDS             <9179..10055
                     /locus_tag="PLI69_01095"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOG41121.1"
                     /translation="KPESRDRRERSERHDRYGKPESRDRRERSERHDRYDKPERKERS
                     KRSKRLELSESVRLNKFIANSGICSRREADEYIKTGLVTVNGEIVTELGTKVKQDDDV
                     RFNGQRLKGEKKVYLLMNKPKDYITTMSDPHAKKSVIDLISPEKCKERVVPVGRLDKA
                     TTGVLLFTNDGDLAARLTHPSFNVKKIYHVFLDKNLKKTDFDALLKGITLEDGEIHAD
                     SLSYVDDDMSQVGIEIHSGRNRIVRRMFEHLGYEIKKLDRVYFAGLTKKNLRRGAWRF
                     LTEEEITYLKMGNFN"
     gene            10128..11009
                     /gene="era"
                     /locus_tag="PLI69_01100"
     CDS             10128..11009
                     /gene="era"
                     /locus_tag="PLI69_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOG41122.1"
                     /translation="MHRSGFVNIIGNPNVGKSTLMNALVGEKLSIVTAKAQTTRHRIM
                     GIVNGDNWQIVYSDTPGILKPHYKLQESMMDFVDVAIGDADIILYVTDVVEKKEKNIS
                     YIEKLKQLECPVIVVLNKIDLSTQEKVVEMMSRWKELLPKAEIIPVSATEKFNLEKLF
                     DTILSHLPVSPPWYDKETFTDKSMRFFASEIIREKILLNYSQEIPYCTEVVIEEFKEG
                     KERYDISAVINVMRNSQKGIIIGNKGAALKKTGTQARIDMEDFFEKKVFLQLFVKVEP
                     DWREDRRKLRNFGYIND"
     gene            11033..>11677
                     /locus_tag="PLI69_01105"
     CDS             11033..>11677
                     /locus_tag="PLI69_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOG41123.1"
                     /translation="MANKTDDFVEEDDLLTEENYSDEESDQDIIVEEDDDVEEDHRFD
                     KVMAGGESKYKLSGMYREWFLDYASYVILDRAVPHIYDGLKPVQRRILHAMKKSDDGR
                     FHKVAGIVGDTMKLHPHGDASIKDALVQLGQKNYLISTQGNWGNIFTGDPAAAMRYIE
                     ARLSKFALEVAFNPKITEYVPSYDGTNEEPIALPMKFPLLLAQGTEGIAVGLAVK"
BASE COUNT         3378 a         2647 c         2580 g         2972 t
ORIGIN      
        1 tgaatccgga tttggacgta cccggggcca ttactcccgg acgattaaat gtagcatcgc
       61 tggagagcgc gctgagctga agtccttccg gcaccttcag gcgaccttcc gagagtttct
      121 ccatatcact gaatatagtt tcgtcagcga ctgaatcctt attccagatg agatattgtt
      181 gcctcatata gtggccgaga gtgcagatca tttcggtctt ggcatcatca tcctcccgtg
      241 ctgcaatgag attgatcata ttctcgatat tacggccgta gcaggaagcc ttcataggct
      301 tctcttcaag ttgtatcttc tcaggacggc tgaaaaactc ctttttatcg ggcttcggat
      361 agggagaatc cacgtctatg tcaaaatctg caataatgta catgtggtcc cagagtttct
      421 gtttatagtc cgggaaatcc ttcatatggg gattgagtat ggccatcacc tggatgacgg
      481 cacgtatctg ttcattacgt ttttcacggt cgggaatcct tttgacctgc tctatcatct
      541 gctgaacatg ccttccgtac tccggcaggt gaagtatatc tctttgcgta ttgtaatcca
      601 tataactttt ttttaaagta ataacctaca aatatagtga tttttagtaa ataatggtct
      661 attttacgac agtttaccca ttattgacaa accggtcatt tgcaatttgt ctaaactggt
      721 gctacttttg taatgcaaat tgcattatac tattaaatca aaagaattat gggaaaaact
      781 cttgtcaccg gatcgctggg acagcttggt agcgaattga gagaactggt cggaaatgac
      841 cggaactgga tatttacgga tgccctacct aacgaagaga ctctccaact ggatatctgc
      901 gacagccggc gtgtgaggga gctgatggag ggacaggatg tggatcttat aatcaattgt
      961 gccgcataca cgaatgtgga caaggccgaa agcgaaaagg aactcagctt taaaattaat
     1021 gccgaaggtc ctaaagtcct cgcagagtgc gttcgtgagc gcaatgccgc acttgtccat
     1081 atctccaccg actatgtatt tgacgggaag cgcaagagag gtgcctatcg cgaaagtgat
     1141 ccctgccgcc cacagagcgt ttatggagca accaaacggc agggagaact tgccatacgc
     1201 cgtagccgtt gccgcggcgt aatcatccgc accgcctggc tctactcctc atacggtaac
     1261 aatttcgtca agacaatggt aagtctcggt agcgaaagaa gtgaaatcgg agtagtagcc
     1321 gaccagatag ggacacccac ctatgcgcgt gatctggcta aagccattct gaccataata
     1381 ccgcaaattg gagagcaccg gggagatatt taccattata caaacgaagg cagctgcaca
     1441 tggtttgact ttgccgcact tataatgacc tattgcggtc ttgactgcaa ggtcaatccg
     1501 cttactacgg aagaatatcc gactgcagca cggagaccgg cctactcggt gttggataaa
     1561 agtaaaataa gggaagaatt cggagtggaa accccttggt ggagcatatc actcagagaa
     1621 gctctcagga agatgggaaa gccggtgcct cagcatctct agctgagaga atcacctcct
     1681 tcggatcaat gccccaatct atggccaacg caggatcatt ccacctgtaa gtgtcctcca
     1741 tttccggtgc atagtaatta tcacacttat actgaaatat ggtattgtcc tccaacacca
     1801 aatatccatg tgcaaatcct cgcgggataa agaacattcg cttgttctca gcactgagct
     1861 ccacagaaac atatttgccg tatgtggggg attcgggacg aatatcaacc gctacgtcga
     1921 gcacacggcc ctgcactacc ctgataagct ttgcctgcgc agcctcaccc ttctggaaat
     1981 ggagtgcccg tatcaccccg cgcttggcgg acaaggactc attgtcctga acaaaattga
     2041 ttttttgcac atttgcctca aactccctca gggaaaatga ttcgaaaaaa taacccctgt
     2101 ggtcgtaaaa cactttcggt tcaataatca caggaccctc tatgcccgtt ttgataaaat
     2161 tcatttcgca atatttttac aaaaataata attattgtaa aattgtgtta cctttgttgc
     2221 acatacaaga aatgattgct atgaacaact gcatattaat atccctctcc ctatcacttc
     2281 ttcttgagaa tgactctcgg gacgggattg ccattatgta ggtttgtcgt ttatacatag
     2341 atgtaatgcc gcccgagcac tcgggcggca ttttttattt aagacttgat tttgataaac
     2401 attaacacaa actataaaac aacaagatta tgaattggga caaactcgct tttgactatg
     2461 tcaaatgcaa caccatggtg tgctccttta ataaaggtga tggatggagt ccgctcgaga
     2521 gcaggacaaa tgacaagtat gaaattacgc tgatgtcggg atctctacat tatgcaatag
     2581 agatatttga ggggcttaag gcctataatg gacgcgatgg aaaaatacgc atcttccgac
     2641 ccggtgaaaa tgccgtcagg atgatccgct ccgctgaatt tctcggaatg cctccggtac
     2701 ccgaagagat gtttacagag gcggttacac gagcggtggc cgaaaacagg gaattcattc
     2761 ctccttatga aagcggtgca tcaatgtata ttcgcccatt tatgatctcc actaacccac
     2821 aactcaccct ggagttggga aaagagttta tgttcgttgt ggcggtaaca ccgacaggca
     2881 atttcaccgg caccagcttc aaccccacga aagctctgct cgtgagggat cacgatcggg
     2941 cagcacctct cggtaccggc tcttataaga taggcgccaa ctacgctgct tccatgctcg
     3001 caggcgtaca ggccaaaaaa gagggctatc cctccatcct ctatctcgat tcaaaagaga
     3061 aaaaatacat agatgaattt agcagttcca actttttcgc cataagggac aacagttaca
     3121 ttactcccga ttctccatcg atcctccctt ccatcaccaa taagtctttg cgtgaaattg
     3181 catcacaaat gggcatgaca gtggaacacc gtccggtgcc tgtggaagaa cttgcaacct
     3241 tcgatgaggc gggagcctgc ggcacggcgc tggtgatcac accggtacac gagatatatg
     3301 acagggagag ggatatccgc tacaacatag gcagtcccgt ccgggttgga gataagagcc
     3361 ggggcctata taaacacctc acaggcatcc agtttggaga gatcgaggac agctttggat
     3421 ggtgccgtat tttatagttt cggggtagtt ttaagttttg ggggttaatt tttgcgaatt
     3481 aaaaagtagg gttacaggca gtttaaggca ctctattttg ttttttaaca aaatagagtg
     3541 gttgatctct tttttcattg caaaaagtgt cataaatttg tatcattcaa catattttgt
     3601 gggaaacgat acaaaaacaa ccatgaagtc caaactgttc attattgcgc tgctcgccat
     3661 agtggcggta ggctgcgaac gcgtcattga tcttccggaa cccaatgtac aagggggaga
     3721 cgttgttacc atagaagcga ccatcccgcc ggaaacccgc gtggcataca ccgattccta
     3781 cacttccggt aacggtggta cactcgcctg gcagacaggt gaccaacttc gactggctgc
     3841 atacaacgga aatacttata ttggaagttc tctctttact ttcgtcaccg gaagcgccaa
     3901 caaatttacc ggcaccttag tggagggcgc taccccccac caccgtttat aaggcctatt
     3961 atcctccgga cgcctataat ttggatgata acggcaatgt acaacctctt gccgccgcct
     4021 tctggcaaca gacgcaggtc gggaatggca atgaccattt aagagataag ctaatattga
     4081 gcgacgtgat agccaacacc atcaatcagt cctttgaatt gctcttaaat agcagcatcg
     4141 tcagattgga actctacaat atgcctcaag atatatatga gctaaaaagt gtgatttgga
     4201 cggttaagac cacatctggt gaaacacgat cggcaattct cgacgtaacc ggggttaccg
     4261 cgccacctac acatccttac cctatcccat tcaacttgaa agcttacctc gcttttgacc
     4321 ctgactttat gcaggtcgcc gtgggtggga aggtaaaaat tacactgatt ggcgacaaat
     4381 cttacgagtg gagtaccacc tcacaagatg gaatgactta cacaaaaggg acgcgctact
     4441 acgctaccgt atccggtaat tggatgcaaa aggttccgtt tatcttcacc atcaaaacca
     4501 atcagtcaaa tcagatactt acactttatc agagggctgc atccaatagc cctgccaatc
     4561 tgacaatcga ctggggagat ggctccggtt ataggacctt caatcaggga gatcctattg
     4621 gagagaccat cgatacgcat aactatgtaa acgcgggaga ttataccgtt accatctact
     4681 ccaataatcc aaattactca agcaaacaga ttccgcaaat cacattttac aaatattctt
     4741 ccgtaaacca gttactgacg gcagttgtta ccccatttcc aaatatgggg gcagatgcaa
     4801 caagtttcga agaatgcttt taccagtgca ctcagttaac tactattccg gcagacttgt
     4861 tcaggtacaa tacgcaagca acgagttttc gcaagtgttt cattttttgc aacggattaa
     4921 cctctattcc atccggactt ttcagttaca atacggcagc aacgaatttc aattcctgtt
     4981 tctatggctg caccggattg agttctattc cggcggaact atttaacaac aatacacaaa
     5041 caatagattt ccgctcttgc tttgctaatt gcaccggatt aaccgcgatt ccggcaaacc
     5101 tgtttacctc taatgatcaa gcacaaattt tcgaaaattg cttcaacggt tgcaccggat
     5161 taaccgcaat accgatgggg ctatttgata acaatctggc agcgactagt ttccatggtt
     5221 gctttgccgg ttgtaccggg ttaaccgcta ttccggaaaa cctattccag tataatacgc
     5281 aagcaaaaca tttcctatgg agcttcggtg gttgtaccgg attaaccgct attccggcag
     5341 ggttgttcag caccaatacc aatgcaatca atttcttagg atgcttcatc aactgcgccg
     5401 gattgactgc tgttcctgca gacctgttca gatataatgt agaagcaact aatttcagca
     5461 actgtttcta taactgcacc ggattgaccg ctgttcctgc tgacctgttc acatataatg
     5521 tagaagcaac aaattttagc tactgtttct acaactgctc cggattgact gctctcccga
     5581 gccacctatt ccgttataat acgaaagcaa cggatttcag ctactgtttc tccggctgca
     5641 taaaattgca attaaatttg gacattttcc caagtccggc acctattttc ggcaaacctg
     5701 acttttttaa tgatagagca atggatttca cagatttttg taaaaatgtg ggttcgcacc
     5761 ctacagccat atccggtacc gcccccaagt tatggagttt taattacggc gccggagtga
     5821 caactaccgg ttgtttcaca ggagccaatg taaccaatgc cggagatatt cccaacaatt
     5881 ggaaatagat ttaggggttg cggggtacgg ggtggagttc cgagttctga tctccgagta
     5941 tttagagtgt ttctctcact atatcggcca attttgaaat caaataaaag ggtagattga
     6001 tcagcctaaa actctttcct gcattggttt taacatcatc aaccccatat ttgcccgacc
     6061 aaattctcac agcaacatta tggttggttt catccataaa ttgatgaatt gatttgagat
     6121 gggcgttatg tccgcttttc acctctaccg gaataatcaa tccatcctga atccaaataa
     6181 aatcaacttc agatgtactg ccacgtttgt tgcgccgcca ataatttcgt ctgaaaccta
     6241 cgtcatttga gagtgctttc aattcctgag cagcaatttg ctcggcagcg gcaccacgcc
     6301 acgtatccat caaatctttt tgcagcagat actctcgttg aataccggca gcgtagttca
     6361 ccaggccggc atcaagccaa aaaagtttag gtgttttttt caggtcggaa gtcgcaggca
     6421 ttattgtatt tgtcgtagga tagactaatt ccagtaacat cgctttactt agtgtgcgaa
     6481 atgcttcgcc tgtttcacgg gctttgtatg gagattcggc aaaaccacca aaagtgatgg
     6541 tttgccctga aaaagcccat ccttcttcta atatataacg gagaacagca gcttgcgtat
     6601 tggtttcggc atacttttca acatcatttt tataagcgtt caatagctcg ttataagttt
     6661 tgccaaggga gacaatatct cggtcagcag cgtaacgagc aaccacttcc ggcattccgc
     6721 cgataagggc atagcgctca aaatgctcaa ttagcggttg atgaaatata ttcacactct
     6781 ctttctcatt aatcacttgt aaaagtccgt tctcatttag ggcaaccaaa tattcggaaa
     6841 aagagcaagg atgaaggcca tatattccac tcgtcccaca ggtaatgaaa tatgcttgtc
     6901 gagcattgtc tccaaaagtg atcccgcagc gatgatgtaa atctcgggag tttcttcata
     6961 gaagtaccgc aataatgaga cggtgtgtgg tgagttttga atctcatcta taaacagcag
     7021 agtgcgtccc tctttttgtg ttttattggc ataaagaaaa agtagcggaa tcaatttttt
     7081 cacttcatcc acactctcca taagattttt agcggcagac ttttcgagat taattgaaat
     7141 gaaattttca aagttttccg caaattgatt tactacggtt gtctttccta cctgccttgc
     7201 accacgtaga atcagtggtt tgcgataggg attttttgcc catttatgca gttgagtttc
     7261 tatgtttcgc ttaaacattg aataataatt tgattttcaa gtgcaaatat aagtaaaatg
     7321 tttcattgca taggtaaaat catgcaattt atgtttcatt gcataggtaa aattacgcta
     7381 tttttgtatc attgcataga taattgggtg tattttgttt cccccgcacc actactccca
     7441 tcttaatgct tctatgggat ccaaaagaga ggcgcggcgg gccggcagat agccggaaag
     7501 gatactgacc actatacata tcagcacaga gagacctagc caacgccagg ggagggcaaa
     7561 ggcacaaccc aacaattttg ccaccaggtt accgaaaagc aaccccataa acagacctaa
     7621 tgcacatcct atctgtccta ccagcactgc ttcagccaga aattgcatgg caatggtaag
     7681 cggtttggca ccgagagctt tgcgtatgcc tatctcgcaa gtacgctcct tgaccgaaac
     7741 cagcagtata ttcatcaacc ccaccgaagc cccgagtaaa gtaatcaatc cgataaccaa
     7801 agccgctatg gagagttttc ccttgagggc atccagctcc ctcattatag aatcgctacg
     7861 actgattgca aaatttgcat catcctgcgg acggagtccc ctgatgagtt tcataaccac
     7921 tacagctcgc tcccatgcct gttcaaaggc ggactcccct cctaccggca ttatatccag
     7981 cgaatagcct gcatcggaag agagcaaacg cgtacgtgcc ggacccaacg ggattaatac
     8041 tgtattgtcc gtaccgatac cgaaaagcgc cccctctctc tccatcacac cggtcactaa
     8101 atactccacc gcacccactc tcagagtctg accaagggga tcgttaccgc tgaagagccg
     8161 tctggcgata ttgtctccta taatgcaaag gtcgcttgag ccctcgatct cacggctgct
     8221 gaaattgcgt ccggaagcca atgaggcaag tccgaggcgt agatgatgtt catccgttgc
     8281 tgttacggtg actgtggggt cgctgattac acttccggcg gagacttgct gcataataat
     8341 cgaagaattg atacttacca ccgctttaga gtcgtatctc ctgacgaatt gccgtacatc
     8401 actccaggag atggcgcgag caaagtgctc atgctcctct tcgagggatg aaatggtaaa
     8461 acagtgtgct cccattcggc caaaactctt ttctacggtc tgcgaaagcc cttcaatggc
     8521 tgtttggatg gagaccagcg aagtgatgcc ggtagcaata ataagtatgg taagtgtgga
     8581 gcgcaatcta ttgtccagaa gagagtcgac tgccatattg aaaaatcctt tcatcctcaa
     8641 tctttttctt tttcaattat gtttcaaaaa taggaaaata atttagatta agttaacttt
     8701 gccgcgcaaa attgcatgta aaatggaaaa cagaaaaaca gaaagaagcc gtaaggagag
     8761 caaaggcgag gcatacggca ataagccggc acgaaaaagc ggtgtcaccg aaaaagcgac
     8821 acgcagaact acacgttcgg cagatcgtcc caaaagagat gatgatgaaa agttctatca
     8881 taaagatgga ggcaaaccca ccggaaaggg tagagctaca gtaaataaaa aaggtgccgt
     8941 aacttcacga catgacagag aggaatggca tgataaacct gacagacgag agcgcagcga
     9001 aaggcatgac agatatggca agcctgaaag ccgtgacaga cgagagcgca gcgaaaggca
     9061 tgacagatat gacaagccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
     9181 agcctgaaag ccgtgacaga cgagagcgca gcgaaaggca tgacagatat ggcaagcctg
     9241 aaagccgtga cagacgagag cgcagcgaaa ggcatgacag atatgacaag ccggaaagga
     9301 aagaaaggtc taaaagatcc aaaagattgg agctgagtga gagtgtacgc ctcaacaaat
     9361 tcatagccaa ttcgggtata tgctccagac gagaggctga cgagtacatc aaaacaggac
     9421 ttgttacagt caatggtgaa attgtcaccg aactgggtac aaaagtaaag caggatgatg
     9481 acgtccgttt caacggacaa cgcctcaaag gagagaagaa ggtatatctc cttatgaata
     9541 agccaaaaga ctatataacc acaatgagcg acccgcatgc aaaaaagtct gttatcgacc
     9601 tcatttcacc tgaaaaatgc aaagaacgcg tagtacccgt aggacgtctc gacaaggcta
     9661 ccaccggtgt attgctattt acaaatgatg gagatttggc ggcacgcctg acacatcctt
     9721 cattcaatgt aaaaaagata taccatgtat tcctcgacaa aaacctcaaa aaaaccgatt
     9781 tcgatgcgct actcaagggg atcacactcg aggatggcga aatacatgca gattcactct
     9841 cctatgtgga tgatgatatg tcgcaagtgg gtatcgagat acattcaggg cgaaatagaa
     9901 ttgttcgaag aatgtttgag cacctggggt atgagatcaa aaaacttgac agagtctatt
     9961 ttgccggtct taccaaaaag aatcttcgcc ggggggcatg gagatttttg accgaggagg
    10021 aaataacata tctgaaaatg ggcaacttta attaaatagc ttttggcttt tggcttttgg
    10081 ccgttagcca ttagcagcca aagagaaaca gcacaaaaca gacaattatg catagatccg
    10141 gatttgtcaa tataataggc aaccccaatg tgggcaagtc cacacttatg aatgccctcg
    10201 taggtgagaa actttccatc gtgactgcca aggcacagac cactcgccac cgcataatgg
    10261 gaattgtaaa cggagataat tggcagatag tctattccga cactccgggc attctcaaac
    10321 cacactataa gctccaggag agcatgatgg acttcgtgga tgttgccatc ggtgatgccg
    10381 acattatcct atatgtcact gacgttgtag aaaagaaaga gaagaatatt tcgtatatag
    10441 aaaagctaaa acaactcgaa tgtcctgtaa ttgtagttct aaataaaata gacctaagca
    10501 ctcaggagaa ggtggtggag atgatgtccc ggtggaaaga gcttctacca aaggctgaga
    10561 ttatacccgt ttccgctacc gaaaaattta atctcgaaaa attattcgat acaattctat
    10621 cccatcttcc ggtaagtccg ccatggtatg acaaagagac cttcacagat aagagcatga
    10681 ggttttttgc atctgaaatc ataagagaga aaattttatt aaattactcg caagaaatcc
    10741 cctactgcac ggaagtggtt attgaggagt tcaaggaggg caaagagaga tacgacatca
    10801 gtgccgtaat caatgttatg cgaaattcgc agaaaggcat catcataggg aacaaggggg
    10861 ctgctctcaa gaagacaggc actcaagccc gtatcgacat ggaggatttt tttgagaaaa
    10921 aagttttcct tcagcttttc gtcaaggttg aacccgactg gagggaagat agaagaaaat
    10981 tgagaaattt tggatatatt aacgactgaa aaataattga gataccctat atatggcaaa
    11041 taaaacagac gactttgtag aagaggatga tctcctgacc gaagagaact attccgacga
    11101 ggaatccgac caggacatta ttgtcgaaga ggatgatgat gttgaggaag accacagatt
    11161 tgataaggtt atggcgggcg gagagagcaa atataaactc tccggaatgt atcgtgagtg
    11221 gttcctggat tatgcttcgt atgtaatcct cgacagagcc gttccccata tttatgacgg
    11281 actcaagccc gtgcaacgcc gcattctcca cgctatgaag aagagcgatg atggccgttt
    11341 ccataaggta gcgggcatcg taggcgacac gatgaaactc catcctcacg gagacgcatc
    11401 catcaaggat gcgctggtac agcttgggca aaagaattat ttgatctcca ctcagggcaa
    11461 ctggggtaat atttttacgg gagaccctgc cgccgcaatg aggtatatcg aggcaagatt
    11521 gagcaagttt gcactcgagg tggcatttaa ccctaaaatc acagaatacg tcccctctta
    11581 tgacggtaca aatgaggagc cgatagccct accgatgaaa tttccgctgc tgctggctca
    11641 aggtactgag ggtattgccg taggactcgc agtcaag
//
LOCUS       DAOSIE010000015        10971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_5886_length_10971_cov_4.850861, whole genome shotgun sequence.
ACCESSION   DAOSIE010000015 DAOSIE010000000
VERSION     DAOSIE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10971
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5886_length_10971_cov_4.850861"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1142
                     /locus_tag="PLI69_01110"
     CDS             132..1142
                     /locus_tag="PLI69_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG41124.1"
                     /translation="MKGRSKHIAIYISLGVALLLLFCGGLLYGSVRIPIGSILEIIAG
                     RFEGRQTWVQIVMQSRLPQTITALFAGASLAVSGLLLQTLFKNPLAGPSILGISDGAY
                     LGVALVMIYFSFNSYLTTILAAFVGAAVVLLIIIGFSRKVQNSVMLLIIGIMVGYLAS
                     SIISILNYHASPDKVHQFVLWGMGDFTGVSMEKLPWFVIFASGGLLGSVFLIKPLNAL
                     LLGESYAANLGVRVKSIRIAILLCTGILTAATTAFCGPISFIGLAVPHIARLLLGSSN
                     HKQLLPITLLAGACVALLCNMLTVVPGSNSILPLNAITPIIGAPVIIYVIVNRRNIQY
                     FN"
     gene            1144..2175
                     /locus_tag="PLI69_01115"
     CDS             1144..2175
                     /locus_tag="PLI69_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG41125.1"
                     /translation="MKRQITISAHGLAIGYSLRGGRRKVIHNSLDMELRNGEVTCLLG
                     LNGAGKSTLIKTLCGLLPPLAGSIRVLGAPLENYSHNRLSTLIGVVLTEKTFAGGITV
                     YDLVSLGRYPHTGIFGQLKESDHEAISRAIESVGIEHKAASYVSELSDGERQKAFIAK
                     VLAQESPIIILDEPTAFLDVTSRIEIMVLLRKLAREQQKSILLSTHDLDSAIQLGDKL
                     WLQERGAKDNRASAPMISGTPEDLILSGVLESFFGKKGVVFDSYSGKISPVPSENPVG
                     IDGEARSAFWAGNALKRKGFAPTTPAKDIVSVQCLSPTEFNVSFPDGKTLKANSIEEL
                     LDLFTNSSS"
     gene            complement(2156..5770)
                     /gene="dnaE"
                     /locus_tag="PLI69_01120"
     CDS             complement(2156..5770)
                     /gene="dnaE"
                     /locus_tag="PLI69_01120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOG41126.1"
                     /translation="MSFVHLHVHSQYSILDGASSIKSLFQKAHKYGQPALALTDHGYM
                     YGVKEFLKVAADYPDVKPIVGCEVYVALEDRHTRRGKIDQKAYHLILLAKNVTGYHNL
                     VKLCSLGNIEGFYFHPRIDRELIEKYHEGLICTTACLGGEVPRHIIEGNMEAAEEAVL
                     WYKSIFGDDYYLEVQSHPTDVPLAPKETFEKQQIVNEALYELAQKHDIKVIATNDVHF
                     VDKEDGPAHDRLICLTFNNNVDDPDRLRYTQQEFLKSKEEMAALFPGHPEVLSNTLEV
                     AEKVESYSIDSDPILPHFPIPEEFEDSDAYLRHLTYKGAEMRYGKEISQKIRDRIDFE
                     LETIQKMGYPDYFLIVQDFIAEARRIDVSVGPGRGSAAGSVVAYCLKITNIDPIKYDL
                     LFERFLNPDRISMPDIDIDFDDEGRSRVFEYVENKYGKDHVAHVVTFGTMATKSAIRD
                     VARIEQVPLQEADRLSKLVPRSFEIDVYDKTDSTSLVKVEVPPTIKLCVERIPEFKEA
                     LDSPDQKLSNTIKYAQQLEGTIRQTGVHACALIIGREDLTNIIPIAVEEDKESKEKIW
                     VSQYEGSYIEQVGMLKMDFLGLKTLSIIKETMSNIVESGHKPFDIEAIPIDDPLTYKL
                     FSKGNTTAVFQFESDGMKKWLRELHPTRFEDLIAMNALYRPGPMEYIPDFVARKHGKQ
                     EITYDLKEMEEYLEDTYGITVYQEQVMRLSQELAGFTPGMADTLRSAMGKKRLSVMEK
                     LYDQFMEGALAKGHPQEILEKIWKDWKSFAEYAFNKSHATCYAWLGYQTGYLKAHYPA
                     QFLAANLSKNLNDIEEITKLMDDCKRMKIEVLGPDINESRTTFAVNKKGNIRFGMAGI
                     KGVGTNVIDAIIAERERGGAFTDIFDFVERLPSGVVNRKVIECLIYSGSFDSFGDMHR
                     TQYFLFNNRDESFIDALLKYTYKYQSDTVMQGGLFGDTEEMKPVRPEVPPLQEYDEIE
                     LLKKEKELVGMYISSHPLDKFKFEFDNLVTVELSEVRNIEDNLSRDPKLRNIEFTVGG
                     LVTDVQIKYTRTGNRPWCTFTLEDFNGSHTFSLSGKSYEAYMKYMVDHTALLIRCVTK
                     EFYRKESTEEKKVPEYRLFIANMTLLSNAKDQFFKEFHISMDLEQSDAEFRKALTKKL
                     RAHKGETPLFIDLSFEHNGQKDCVSLYSDKFRVSVDGELVDWIYEQGLDCRAVKKMNW
                     "
     gene            complement(5799..7316)
                     /gene="gpmI"
                     /locus_tag="PLI69_01125"
     CDS             complement(5799..7316)
                     /gene="gpmI"
                     /locus_tag="PLI69_01125"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOG41127.1"
                     /translation="MNKKVLLIVMDGWGEGRQDYSNAIYTAGTPEIDALKAKYPFSQL
                     SASGEDVGLPEGQMGNSEVGHLNIGAGRIVYQDLVKINIACREHALMSNKEIAAAYDY
                     VKKSGKQLHFMGLCSHGGVHSSLNHLYEFLREAANAGLEKVFVHCFMDGRDTDPRSGK
                     GYVAELEEVLSRTTGKIASVVGRYYSMDRDKRWERIKEAYDLLVSGKGRKAISAVEAI
                     EESYREEVTDEFIKPISIVSEKGTPVATLEEGDAVIFFNFRNDRAREITYVLTQADMP
                     EAGMHTIPLYYCTLTPYDDKFEGLHLLFDKDNVPQTLGEVVSSVGKSQLRIAETEKYA
                     HVTFFFNGGREKEFAGEDRILINSPKVATYDLKPEMSAPEVKDALVADLATGKHDMII
                     LNFANGDMVGHTGVYDAIVKAVRTVDSCVGEVVRAARKAGYSVLITADHGNADHAVNE
                     DGTPNTAHSLNKVPFIVVDDDVKEVRAGILADIAPTMLKLMGIPQPEIMTGRSLI"
     gene            7388..9442
                     /gene="rnr"
                     /locus_tag="PLI69_01130"
     CDS             7388..9442
                     /gene="rnr"
                     /locus_tag="PLI69_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOG41128.1"
                     /translation="MGTKRKKNDFSGSKSSKSDKKNRRKMQDPVKASDRRSKSKVEAE
                     GIINLTRDGFGFVTVEGIPNDIFIHISKMRGALHGDRVKILIPKNIDHRVRRIEGEVV
                     HIIERSRKPHIGVLHIVGKQVWAIVESRNMPYDIRIPVDSLDELPEMGGIKAAKGIKV
                     AVLVTDWPRRSPEPLGKIVDVLGVPGDNDTEMHSILAQYNLPYRFEPEVDSAANAISD
                     VITADEIAKRWDYRSVTTFTIDPADAKDFDDALSIRELENGICEVGIHIADVTHYVTP
                     DTPVDKEAFNRATSVYLVDRTIPMLPEKLSNNLCSLRPHEDKLCYSVIFKIDDNAKVL
                     DAKFGRTIINSDHRFDYEQVQEIIQNMHGPLCKEILKLHSIATILRRKRFEHGAIKFE
                     RPEMKVIVDKEGKPLDVCRQESVESNWLIEEFMLLANRYVAFQVARKCKSVNPTFVYR
                     VHDNPNPDKLRELRRFAKNFGHTLGDTDNPKNIARSLNKLMTGIKDRPEENAIEMMAL
                     RSMSRAVYSTDNIGHYGLAFQFYTHFTSPIRRYPDMMVHRLLTKYLNGAKSQDKAYYE
                     ENCKYCSEREQLATDAERASIKLKLVEFMQDKIGQEFEGTVSGLTEWGMYVEIEPTKI
                     EGMVSLREFTKDYLVFDEEKYFIVAKASGRKFTLGDKVKVRVLRANMEQKLIDYELIW
                     EE"
     gene            9637..10191
                     /locus_tag="PLI69_01135"
     CDS             9637..10191
                     /locus_tag="PLI69_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41129.1"
                     /translation="MRKKYFILLFLAVLLCSSCVETIHPESVEEAPVVVNCVLDTSDT
                     QSLYLAYSSKVSEVEDFKIIDNADAVLREIKGNDTVTVATFSFESDGMWRTNFRPVGG
                     KEYQLLIEIPGQKRVTAITQMPEIITIYHGIGSSSSIGEISRYPGSSYWSFFANNTLY
                     LSMFGKDNEGNIVIADEIATDHLF"
     gene            10204..10590
                     /locus_tag="PLI69_01140"
     CDS             10204..10590
                     /locus_tag="PLI69_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41130.1"
                     /translation="MTGKTFTDLSKLPILYTTVEGKPLHKQYIRIPNPDKYINPNYNS
                     GKEEYKGQFTVATNEKSIRNLTILQVSDELDAYYKDVLTYQNKYEVMDNLLIVYDRAN
                     VYNNVTNAIGIFGAYNKYILPWAPLL"
     gene            10587..>10971
                     /locus_tag="PLI69_01145"
     CDS             10587..>10971
                     /locus_tag="PLI69_01145"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HOG41131.1"
                     /translation="MKRIYYIAILFLIFIGCGSLSAQEAVPIRDAIKQSVIVDSRIMR
                     QQQGLTTIQIEAIKGIISPAGEGDAIKFIQTLPGVSTGAEGTSSYYVRGGNSGNNAVT
                     LDGVPIYGISHLMGMTSTIPAEIVSS"
BASE COUNT         2884 a         2694 c         2513 g         2880 t
ORIGIN      
        1 cactacccga accacctatt acgacgatat aactctccat cccgaccgtc ttctggaaga
       61 tttgatctat atttatcatc cggaattgct tcccggatat gttctgagat attatgcccc
      121 tatgaaatga gatgaaagga aggagcaaac atatagcaat ctatattagc ctcggcgtag
      181 ctctgctgct gcttttctgt ggcggactgc tctatggttc ggtcaggata ccgataggtt
      241 ccatattgga aattattgcc ggcagatttg aaggtcgtca aacctgggta caaatcgtta
      301 tgcagtcgag actgccccag acaataacgg cgctatttgc cggagcctct ctggctgtaa
      361 gcggattgtt gttacagact cttttcaaaa atccccttgc aggcccttca attctgggta
      421 tcagtgacgg agcctatctc ggcgtggcgc ttgtgatgat atatttctct ttcaacagtt
      481 atcttacaac gattctcgca gcatttgtag gagcagcggt cgtgctgctt atcatcatag
      541 gattttcccg aaaggtacaa aacagcgtca tgctgctcat aatcggtata atggtgggct
      601 atctcgcctc ctcgatcatc tccatcctca actatcacgc ttcccctgac aaagtgcatc
      661 aatttgtact ctgggggatg ggcgacttca ccggagtctc gatggagaaa cttccgtggt
      721 ttgtgatatt cgcctccgga gggctgttag gctcggtttt tctgataaag ccccttaatg
      781 cgctgttgct cggagagtca tacgctgcca atctgggcgt gcgcgtcaag agtatccgca
      841 tagcaattct tctctgtacc ggtatcctta ctgccgctac tactgccttc tgcggcccca
      901 tctcctttat cggtctggcc gtacctcata ttgcgagact gcttctcgga agttcaaacc
      961 ataagcagct gctgccgatt accctgcttg caggtgcctg cgtagccctt ctttgcaaca
     1021 tgctgacagt ggtgccggga agcaactcca tactaccgct caatgccatc actccgatta
     1081 taggagcgcc ggtcatcata tatgtgattg tcaaccgaag gaatatccaa tatttcaatt
     1141 gaaatgaaac ggcagataac catatcggca catggactgg ccatcggata ttctctgagg
     1201 ggcggccggc gcaaggtgat ccacaactcg ctggatatgg aacttcgcaa cggtgaagtg
     1261 acctgtctgc tcggcctcaa cggtgccggc aaatccactc tcatcaagac cctatgcggg
     1321 ctgcttccac ccttggcagg cagtatcagg gtattgggag caccacttga gaactactcc
     1381 cacaaccgtc tatcaactct gatcggggtg gtactcacgg aaaagacctt tgcaggaggc
     1441 attaccgtct acgatttggt ctccctcggc agatacccgc acacaggcat cttcggacag
     1501 ctcaaagaga gcgaccacga agccatttcg agagcaattg agtcggtagg catagagcac
     1561 aaggccgcct cttacgtctc agaactgagc gatggagagc gccaaaaggc ctttatcgcc
     1621 aaagtgctgg cccaggagtc cccaatcatc atactagacg aacccactgc ctttctggat
     1681 gtaacaagca ggatagagat aatggtgctt ttgcgtaagc ttgcgcggga acagcaaaag
     1741 agcatcctgc tctccaccca cgatctggat tccgcaattc agctcggaga caaattgtgg
     1801 ctgcaagaga ggggggcgaa ggacaaccga gcctctgcac cgatgatcag cggcacaccg
     1861 gaagatttga ttctcagcgg tgtgctggag tctttctttg gaaaaaaggg agtggttttc
     1921 gactcctatt cgggaaaaat atctcccgtc ccttcggaaa atccagtagg catagacgga
     1981 gaggcacgaa gtgcattctg ggcgggaaat gctctgaagc gcaaagggtt cgctcccacg
     2041 actcctgcca aagacatcgt ttctgtgcaa tgtctctcac ccaccgagtt taatgtttca
     2101 tttccggatg gaaaaaccct caaggcaaat tccatagagg agttgctgga tctctttacc
     2161 aattcatctt cttgacggca cgacagtcga gaccctgctc gtaaatccaa tccacaagtt
     2221 ccccatctac cgatactctg aatttatcgg aatagagcga cacgcaatcc ttttgcccgt
     2281 tatgttcaaa tgaaagatcg atgaaaagag gtgtttcccc tttatgagca cgcaatttct
     2341 ttgtcaaggc tttacggaac tcggcatccg actgctccag atccatcgat atgtgaaatt
     2401 ctttgaaaaa ttgatctttt gcgttggaga gaagagtcat attggcgata aagagacgat
     2461 attccggcac ttttttctcc tctgttgact ccttgcggta aaactctttc gtgacgcatc
     2521 tgatcagtaa agcagtatga tccaccatat acttcatata tgcctcatag ctcttgcccg
     2581 aaagggagaa ggtgtgactc ccattgaaat cctctaaggt gaatgtacac caagggcgat
     2641 ttccggtacg ggtatacttg atctgtacgt cggtaacaag gccccctacc gtaaattcta
     2701 tattccggag tttgggatcc ctcgagaggt tgtcctctat attacgcacc tcgctaagtt
     2761 caaccgtcac caaattgtca aattcaaatt tgaatttatc caacggatga gaggagatat
     2821 acatacccac aagttctttt tcctttttga gaagctctat ttcgtcatac tcctgaagag
     2881 gcggtacttc gggacgcacc ggcttcatct cttcggtatc cccgaagagg cctccctgca
     2941 tgaccgtatc gctctgatac ttgtaggtat acttgagcag agcgtcaata aatgactcat
     3001 ccctgttgtt gaacaagaaa tactgcgtcc ggtgcatatc accaaaggag tcgaacgagc
     3061 cggaatagat cagacactct ataaccttgc ggttgaccac tccggaaggg agcctctcca
     3121 cgaagtcaaa gatatctgta aatgctcctc cccgttccct ttccgcaatg attgcatcta
     3181 tgacattggt tcccacaccc ttgattcctg ccattccgaa acggatattt cccttcttgt
     3241 tgacggcaaa tgtggtgcgg ctctcgttga tgtcgggtcc gagcacttcg attttcatcc
     3301 ttttgcaatc atccatcagc ttggtgattt cctcaatgtc gttgaggttt ttactgaggt
     3361 tagcagccag gaactgagca ggatagtgtg ccttgagata accggtctga tagcccaacc
     3421 aggcataaca ggtggcgtgt gacttgttga acgcatactc tgcgaaactc ttccagtcct
     3481 tccagatctt ttccaaaatc tcttgcgggt gacctttggc cagagctccc tccataaact
     3541 gatcgtagag cttttccatc acactgagtc tctttttgcc catggcactg cgcagagtgt
     3601 cggccatacc gggagtaaat cctgcaagct cctgagaaag acgcatcacc tgttcctgat
     3661 ataccgtaat cccataggtg tcctcaagat actcttccat ctccttcaag tcgtatgtta
     3721 tctcctgttt gccatgttta cgtgctacaa aatcggggat atattccata gggccgggac
     3781 gatagagtgc attcatcgca atcaggtctt caaaacgggt cggatgcagt tcgcgtaacc
     3841 attttttcat accatccgac tcaaactgga agactgccgt agtattgccc ttactgaaaa
     3901 gcttgtaagt aagaggatcg tcgatgggaa tggcttcgat gtcaaaaggc ttatgaccgc
     3961 tctcaacaat gttggacatc gtctctttga taattgaaag agtcttcaga ccgagaaagt
     4021 ccatcttgag catacctacc tgctcaatat aggatccctc atactgggaa acccatattt
     4081 tctctttgct ctccttatcc tcctcaactg ctattggaat aatattggta aggtcctcac
     4141 ggcctataat cagcgcacag gcatggactc ctgtctgacg gatggtgcct tccagctgct
     4201 gggcatactt gatggtgttg ctcaattttt ggtcgggaga atcaagggcc tccttaaact
     4261 caggtattct ctccacgcag agctttatag tcggaggtac ctcgaccttg accagcgacg
     4321 tagaatcggt tttgtcgtaa acatctatct caaatgatct ggggacaagt ttgctcagac
     4381 gatccgcctc ctggagaggc acctgctcga tacgggcaac atccctgatg gcgctcttgg
     4441 ttgccatcgt accgaatgtc accacatggg caacatggtc tttaccatat ttattttcca
     4501 catattcgaa aaccctggaa cgtccctcat catcgaaatc tatatctata tcgggcatcg
     4561 aaatacggtc gggatttagg aaacgctcaa acagaagatc atatttgata gggtcgatat
     4621 tggttatctt aaggcaatat gccaccacgg atcccgccgc agagccacgt ccggggccta
     4681 ccgaaacatc tattctacgc gcttctgcaa taaaatcctg cactatgagg aaatagtcgg
     4741 gatatcccat cttttggatg gtttcaagct cgaaatcaat cctgtcgcgt atcttttggg
     4801 aaatttcttt cccgtagcgc atttccgcac ctttataagt gaggtgtctc agataagcat
     4861 ccgaatcttc aaactcttca ggaattggga aatggggcag aatgggatcg gagtctatgg
     4921 aataggactc aactttttcg gctacttcga gagtattgga caacacctcc ggatggccgg
     4981 gaaagagagc cgccatctcc tctttgcttt taagaaactc ctgctgagtg taacgcaacc
     5041 tgtcgggatc atcgacattg ttgttgaacg tcagacaaat gagacgatca tgtgcagggc
     5101 catcctcctt atctacaaaa tggacgtcat ttgtggctat aaccttgatg tcgtgctttt
     5161 gtgccagctc atagagagcc tcattgacaa tctgctgttt ctcaaacgtc tctttcgggg
     5221 caagcggtac atccgtggga tggctctgaa cctcgagata atagtcatcg ccgaaaatgg
     5281 atttatacca aagtacagcc tcttccgctg cctccatatt tccttcgata atgtgccttg
     5341 gcacttctcc tcccagacag gcggtggtgc aaatcaatcc ctcgtgatat ttttcaatca
     5401 gttctcgatc tattctcgga tgaaaataaa aaccctctat attgcccagc gagcagagtt
     5461 ttaccagatt gtgatatccg gtcacattct ttgccaaaag tatcaggtga taggcctttt
     5521 ggtcaatttt cccccgtctg gtatgtctat cctccagtgc cacataaacc tcgcatccga
     5581 ctatgggttt tacatcggga tagtcagccg ccaccttcag aaactccttt acgccataca
     5641 tataaccatg gtcggtcagt gcaagggcgg gctgtccata cttgtgcgcc ttttggaaaa
     5701 gggactttat agaagaggct ccgtccagaa tggaatattg cgagtgtacg tgaaggtgga
     5761 caaatgacat tggaaaatgc tcggttaaat gttgataatt atattagtga acgacccgtc
     5821 ataatctcgg gctgaggaat gcccatcagt tttagcatgg tgggggcaat atcggccagg
     5881 atgccggctc taacctcttt cacatcatca tctaccacta taaagggcac tttgttgagt
     5941 gagtgagccg tattgggagt gccgtcttca ttgacggcat ggtcggcatt gccgtggtca
     6001 gcagtgataa gtaccgaata accggcctta cgggccgccc tgaccacttc gcccacacag
     6061 gagtcgaccg tccttaccgc cttgacaatg gcatcataga caccggtatg tcctaccata
     6121 tctccgttgg cgaaattaag tattatcatg tcgtgtttgc ctgtggccag atcggctaca
     6181 agagcatcct ttacctcagg tgcgctcatc tccggcttca gatcataagt tgccactttc
     6241 ggggaattaa tcagtatgcg atcctctccg gcaaactctt tttcacgccc gccgttgaag
     6301 aaaaatgtca catgtgcata tttttccgtt tcagcaatgc gaagctgact ctttccgaca
     6361 gaagaaacca cttcaccgag agtttgaggc acgttgtcct tatcgaaaag gagatgaagt
     6421 ccctcgaatt tgtcatcata gggagtcaga gtgcaatagt agaggggaat ggtatgcatt
     6481 cctgcctcag gcatatcggc ctgggtgaga acgtaggtta tttcgcgggc gcgatcattc
     6541 ctgaaattga agaaaataac cgcatcaccc tcctccaggg tggctaccgg agtccctttt
     6601 tccgacacta tggagatggg ctttataaat tcgtcagtaa cctcttcccg ataggattcc
     6661 tctatggcct caacagctga aatggccttc cggcctttac cgctgacaag tagatcatag
     6721 gcctccttga ttctttccca tctcttgtcc ctatccattg aataataacg gcccaccacg
     6781 gaggctatct taccggtagt gcgtgaaaga acctcctcca actcggccac atagcccttt
     6841 ccacttctcg ggtcggtatc acgaccatcc ataaagcaat gcacaaatac cttttccaga
     6901 ccggcattgg ctgcttcgcg taagaattca taaagatgat tgagagaact gtgtactccg
     6961 ccatgcgaac aaagtcccat gaaatggagc tgtttacccg atttttttac ataatcgtat
     7021 gcagccgcaa tctccttgtt ggacatcagg gcatgttcgc gacaagcgat attaatttta
     7081 accaaatcct gatatacaat gcggccggct cctatattga ggtgtcccac ctcggaattg
     7141 cccatctgcc cttcgggaag accaacgtcc tcgccgctcg cactgagctg cgaaaaggga
     7201 tacttcgcct tcaaagcatc aatttcaggg gttcctgccg tatatatcgc atttgaatag
     7261 tcctgacggc cttctcccca gccgtccatc actatcaaga gcactttttt attcattatc
     7321 tattctattt ttactaaatt tgttacaata tttcgtccga tcggacgaat gtacaaagtt
     7381 acgaattatg ggaacaaaga gaaagaaaaa tgatttcagc gggagtaaat cttccaaaag
     7441 cgataaaaag aatagaagaa aaatgcagga tcccgtcaag gcatccgaca ggcgaagcaa
     7501 aagtaaagtc gaggctgaag gcataatcaa tctcaccaga gacggattcg ggtttgttac
     7561 cgttgaaggt attcccaatg atatattcat ccacatcagc aagatgcgtg gcgctctgca
     7621 cggggaccgc gtcaaaatcc tcattcccaa aaatatagac cacagagtgc gcaggattga
     7681 aggcgaagta gtgcacatca tcgagcgttc gcgaaaaccc catatcggag tgctgcacat
     7741 agtcggaaag caggtctggg ccatagtgga gagccgcaat atgccctacg acatccgcat
     7801 ccccgtagat agcctcgatg aattaccgga aatgggcggc atcaaggcag ccaaagggat
     7861 caaggtagcc gtgctggtga ccgactggcc gcgccgcagt cccgaacctc tcggcaaaat
     7921 agtggatgtt ctcggagtcc cgggagataa tgataccgaa atgcactcca tccttgcgca
     7981 atacaatctc ccctaccgct tcgaaccgga ggtggatagt gctgccaacg ccatctcaga
     8041 tgtaataacc gccgatgaga tcgccaaacg gtgggactac cgctccgtca ccacctttac
     8101 cattgacccc gcagatgcca aggactttga cgacgctctc tcgataagag agctggaaaa
     8161 tggcatctgt gaggtgggaa tccacattgc ggacgtgacg cactatgtga ctcccgatac
     8221 tccggtggac aaagaagctt tcaaccgcgc aacttccgtc tatctggtag acaggaccat
     8281 accgatgctc cccgaaaaac tctccaacaa tctctgttca ctgcgtccgc acgaggataa
     8341 actctgttac tcggtcattt ttaaaattga cgacaacgca aaggtgctgg atgccaaatt
     8401 cggacgcacc atcatcaact ccgaccacag atttgattac gagcaggtgc aggagatcat
     8461 ccagaatatg cacggacctc tctgcaaaga gatactcaag ctccactcca ttgccaccat
     8521 cctccgcagg aaacgcttcg aacatggtgc aataaagttt gaacgtcccg agatgaaggt
     8581 aatcgtggac aaagagggca aaccgctgga tgtatgccgc caggagtcgg tagagagcaa
     8641 ctggcttata gaggaattta tgttgttggc taaccgttat gttgcttttc aggtagccag
     8701 aaaatgcaaa tcggtaaacc ccacttttgt ttaccgcgta cacgacaatc ccaatcccga
     8761 caaactacgg gagctgcgcc gctttgcgaa gaatttcggt cacaccctcg gcgataccga
     8821 caacccgaaa aatatcgcca ggagcctcaa caagcttatg accggcatca aagatagacc
     8881 tgaagaaaat gcaatcgaga tgatggcact ccgctctatg tcccgtgccg tctattccac
     8941 cgacaatatc ggccactatg gtctggcatt tcagttctat acacatttca cctctcccat
     9001 caggcgctat cccgatatga tggtgcatcg tttgctgacc aaatacctca acggagccaa
     9061 atctcaggac aaagcctatt atgaggagaa ttgtaaatat tgctccgaga gggaacagct
     9121 ggctaccgat gccgaacgcg ccagcatcaa gctcaaactc gtcgaattta tgcaggacaa
     9181 gatcgggcag gagtttgaag gcactgtctc aggtctcacc gaatggggaa tgtatgtgga
     9241 aatcgagccc accaagattg aaggtatggt ctcattgagg gaatttacca aggattatct
     9301 ggtgtttgat gaggaaaaat attttattgt cgccaaggcc tccggccgca aattcaccct
     9361 cggagacaaa gtcaaagtgc gcgtcctccg cgcaaacatg gagcagaagc ttatagatta
     9421 tgaactgatt tgggaggaat gaaaatggga cgagtcccat tttagttctg agtcggtagt
     9481 gtgtagtttc gagtaggtag tgggagtctg tagtggtgcg agttacgcgt tgcgaggtgc
     9541 ggggcgcaaa ttgtagggag tcatatagct ctgagtatgg agtattgcat ttgaagttgg
     9601 ctgtttgcgg ttggcggtat tcgatttaat gccattatga gaaaaaagta tttcatatta
     9661 ctatttttag cagttttgct atgctcttcc tgtgtcgaga ccatccatcc ggagtcggtt
     9721 gaggaggcgc cggtagttgt gaattgtgtt ttggacacat ccgatacgca atctctttat
     9781 ttggcttatt ccagcaaagt cagcgaggtt gaagatttca aaataattga caacgctgat
     9841 gcggtattga gagaaataaa aggcaatgat acggtcactg tggccacatt ttcttttgaa
     9901 agcgatggta tgtggagaac caatttccgt cctgtaggtg ggaaggaata tcaactgctg
     9961 atagagattc cgggacaaaa gagagtaacc gctattaccc aaatgccgga aataataaca
    10021 atttaccatg gtattggttc ctccagctct attggagaaa tatcacgtta tcccgggtct
    10081 tcatattggt ctttttttgc taataataca ttgtatttat ctatgttcgg aaaagataat
    10141 gaaggaaata tagtaatagc tgatgagatt gcaactgacc atctgttttg agaccaattc
    10201 aatgtgacgg gtaaaacttt tacagatctg tcaaaattgc cgatattata tacgactgtt
    10261 gaaggaaaac ctctacacaa acaatatatc agaataccca atcctgacaa atatatcaat
    10321 ccaaactaca atagtggcaa agaagaatat aaaggccaat tcacggttgc cacaaatgaa
    10381 aaatctatac gtaaccttac tattttgcag gtttctgatg aattggatgc ttattataag
    10441 gatgttctta catatcaaaa caaatatgaa gtaatggaca atttgcttat tgtgtatgat
    10501 agagctaatg tatacaataa tgttaccaat gccataggaa tatttggcgc ttataacaaa
    10561 tatatactgc catgggctcc attgctatga aaagaatata ctacattgcc atactatttt
    10621 taatatttat cggctgcgga agcctctccg ctcaggaagc tgttcccata agggatgcca
    10681 tcaagcagtc cgtcatcgtt gactcgagga taatgaggca gcaacaggga ttgaccacca
    10741 ttcagataga ggctatcaaa ggaataatat cgcctgccgg tgagggcgat gccatcaaat
    10801 tcatccagac cctgccggga gtctccacag gtgcggaagg cacatcctct tattatgtta
    10861 gaggaggcaa ttccggcaat aatgccgtta ccttggatgg agtcccgatc tatggcataa
    10921 gccacctcat gggaatgacc tcaacaattc ccgcagaaat tgtaagttca g
//
LOCUS       DAOSIE010000016        10885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_5940_length_10885_cov_4.257064, whole genome shotgun sequence.
ACCESSION   DAOSIE010000016 DAOSIE010000000
VERSION     DAOSIE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10885
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5940_length_10885_cov_4.257064"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PLI69_01150"
     CDS             complement(<1..755)
                     /locus_tag="PLI69_01150"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOG41132.1"
                     /translation="MNNILESDIKFLQGVGPKRAALLQKELGISTFRDMLYTFPFRYI
                     DRTRFYSIREIDSSSAYIQLRGSIRDIRTVGEGRAKRLIAYLYDGTGTIELVFFQGIK
                     WISGRLKIGAEYIVFGKPSIFNQTYNMVHPEIDPATEDKLAMVGTLIGVYSSTERLRS
                     ACIGNKVFAKLQYNLINQVIESIEETLPRSLLLRKRFISLRQALVNIHFPTDLQILDK
                     AKERLKFEELFYLQLTLLKQKNIRMRGSTGILF"
     gene            828..1907
                     /gene="pdxA"
                     /locus_tag="PLI69_01155"
     CDS             828..1907
                     /gene="pdxA"
                     /locus_tag="PLI69_01155"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOG41133.1"
                     /translation="MSRKIVVGITQGDSNGIGYEVIIKALSDARMLDMCIPVVYGCSR
                     TFGMYRKLVPETEQITTNIINSASEARPKHVNIVNAVPDSLAVDVGQPTFDGARAAII
                     ALKAAVADVKANFLDAIVTAPFSKHTIAQTEFSFPGHTEYLINEFGVEDGLMFLCADN
                     LRVGVVTNHLPLSKVSSAITKEVLMSKIRLMNHSLIRDFGIVRPKIAVLGLNPHAGDG
                     GSLGTEEIDTIIPAIRESNVENILAFGPYSPDGFFATNTQYKFDAVLAMYHDQGLIPF
                     KALAFDKGVNFTAGLPIVRTSPDHGTAFEIAGKSMANPGPMISAIYTAIDICNNRDNY
                     DEMSANPLEIKHFEGPKHERTILPE"
     gene            1911..2408
                     /gene="rnhA"
                     /locus_tag="PLI69_01160"
     CDS             1911..2408
                     /gene="rnhA"
                     /locus_tag="PLI69_01160"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOG41134.1"
                     /translation="MAKRTPIQIYTDGACRGNPGPGGYGVVMRCGNYEKEFSAGYRLT
                     TNNRMELLAVIIGLEAIKWSDAAVTVWSDSSYVVNAVENGSVFNWEKKNFLKKANPDL
                     WIRFLKVYREHKVSFVWIKGHSGHPDNERCDYLAVTASMGPDLAMDEVYETSNVKNEL
                     FNNLK"
     gene            2415..3101
                     /locus_tag="PLI69_01165"
     CDS             2415..3101
                     /locus_tag="PLI69_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HOG41135.1"
                     /translation="MKKYFAEVIGTMVLVLLGCGSAVFNGGVGTTAQVLTVSFAFGLS
                     VVAMAYAIGSISGCHINPAITLGVMLTGRMKTGEGLKYMLAQVIGAFIGSALIFALVQ
                     TGDKDAAMYATTTGANAVSNSASVGSALIAEMVGTFIFVLVVLGVTDSKKGNPALAGL
                     AIGLTLVLIHIVLIPITGTSVNPARSIAPAVFEGGQALKDLWIFIVGPFVGAALSAVV
                     WDGLAKETAK"
     gene            3290..3991
                     /locus_tag="PLI69_01170"
     CDS             3290..3991
                     /locus_tag="PLI69_01170"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOG41136.1"
                     /translation="MKHNGLTLGVLGGMGPAATAEFLRLLAEKAPADVDQEHPRMIVY
                     SHTITPDRTTFILGKGPDPHNYIREGLDTLIGWGADLLAVTCNTAHYFIDKFRDELPV
                     PVIHIIEETIKKSQAVCPEGAWLTATLGTMRTGLYQKHSAYFGYDFKLPSEEMQVEIH
                     DITDQVKAGKHREAGVRFRKVVEQLWEIEKLPVVGACTEIPIAYSHTGLPEEMGISCL
                     EALAEGCIRELYRPL"
     gene            4037..>5019
                     /locus_tag="PLI69_01175"
     CDS             4037..>5019
                     /locus_tag="PLI69_01175"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOG41137.1"
                     /translation="MRRIVKYTVLMAIMLMAASCGDPKPSTGPDNPSKVNFAKGADVS
                     WVTQMERDGVKFYNSKGTEMECMALLKSLGMNTIRLRVWVNPVDGWNGKADLLVKAKR
                     AKALGMRLMIDFHYSDSWADPGKQNKPAAWVNLPFEELKGALVSHTIEILEALKGESI
                     YPEWVQVGNETSDGMLWEDGRASVNMKQYTDLHNAGYRAVKEVFPEAKVILHIDRGDS
                     GAHYNWLLDGMAPYLPQYDIIGMSLYPDPGNWQYINEACLINMKELISKYNKDIMICE
                     VGMSWDKPEICKGFLIDLIDKALEKTDGRCLGVLYWEPQGFVNWSRYTKAA"
     assembly_gap    5020..5029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5085..7586
                     /locus_tag="PLI69_01180"
     CDS             5085..7586
                     /locus_tag="PLI69_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOG41138.1"
                     /translation="MKKYIPLALLLSIFAAVSCHKGNPRSEILLEEGWKFSIGDFDTA
                     SDPAFDDSEWQEVRIPHDWAIYGPFERSNDLQVVAVRQNDEIIATEITGRTGGLPYVG
                     VGWYRTEFDLRGFDGEKEAVSLLFDGAMSGAQVWVNGTKVITWPLGYNSFHCDITQWI
                     NEDGEDNSIAVRLENLPKSSRWYPGAGLYRNVRLVRTSKLHIPIWGTYITTPEVSRKK
                     AEVSMQIAVEGSTDKELRIVTEIRNPKGKKVAYSESLVEELNDQTDTVMLRFTVRRPD
                     LWSPESPALYTAHTTIYEGDLLTDSHVTRFGIRYTEVVEGKGFYLNGESRKFKGVCNH
                     HDLGPLGAAVNKAALRHQLEMLKDMGCDAIRTSHNMPAPELVDLCDEMGFMLIVEAFD
                     EWDIAKCTNGYHLYFEEWAEKDMVNMIRNFRNNPSVVMWSIGNEVPTQCDDQGYKVAA
                     FLQSICHREDPSRLVTCGMDQVQCVLANGFAQLMDVVGLNYRTHIYKEAFETLPQGNI
                     LGSETSSTVSSRGVYKFPVALKAGAKYDDHQSSSYDVEYCSWSNIPDVDFALAEDFEW
                     TMGQFAWTGFDYLGEPTPYDTDSWPNHSSLFGIIDLASIPKDRYYLYRSQWNENDNTL
                     HLLPHWNWEGREGETIPVMCYTDYPSAELFVNGVSQGVVTKGSYSLADIDLSKMDKDA
                     LMARYRLIWPDVVYQPGEIEVVAYNAKGREQMRKRIVTAGEPHAIVLEANRNKIEADG
                     RDLAYVYVSIVDKDGNLCPNASNEVRFEVKGAGEFVAVANGDPTCLYPFHEPIIPAFS
                     GRLTAIVTSHDYRGSMSVKAFSEGLEPAQIIIKTN"
     gene            7598..8749
                     /gene="galK"
                     /locus_tag="PLI69_01185"
     CDS             7598..8749
                     /gene="galK"
                     /locus_tag="PLI69_01185"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOG41139.1"
                     /translation="MIDSKILKKAIKDKFGHSDFSLFSAPGRINLIGEHTDYNGGFVL
                     PASIDKCISLAIVPNGTSTVNLYSIDYDESVSFDVDGAKPEQQWASYVYGVIQEMKKR
                     GASPKGFDAVFGGDIPLGAGMSSSAALESVFGTALNTIFKLGFDKRELAKIGQMTEHN
                     YVGVRCGIMDQFASLFGEAGKVIRLDCRSLEYELFPFAPEGIRLVLIDTMVKHSLASS
                     EYNVRRAQCEAGVAVISRYKEGVELLRDVTMEDLDADKGEMDPEVYRRCHFVISENQR
                     LLDGCEDLSRGDYALFGQKMFRTHEGLSKEYGVSCEELDFIVEIARGVEGVLGARMMG
                     GGFGGCVLAMVKNENHDEYVAKVREAYQKRFGKDPRVIDVVISDGARQW"
     gene            8743..9762
                     /locus_tag="PLI69_01190"
     CDS             8743..9762
                     /locus_tag="PLI69_01190"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOG41140.1"
                     /translation="MVDSDIKYFTLTNSSGASVILSNLGAGIISVIVPDKDGNMADVA
                     LGYAKPEDYFCDGPCMGKVPGRYANRIAFGKFSLSGKEYSLSINNGPNHLHGGPGEEC
                     WANRIWFGVQNGNEVSFALESGDGDAGYPGNVMAEVIYRWDEESRLTITFRAKSDADT
                     IINLTNHAYFNLKGEGRGDIKDHTLQLEASNYLPTDETLIPAGSPAPVEGTPMDFRTP
                     VKLGEHLFDDFPALRYGKGYDNCWAVDGWQKGVVRKVAELANPASGRKLIVSTDAPGI
                     QIYTGNWLKGSPKSKSGAEYDDYYGVALECQSFPDSPNRPDFPSTLLAAGDTWQRVIE
                     FHFTK"
     gene            9759..>10885
                     /locus_tag="PLI69_01195"
     CDS             9759..>10885
                     /locus_tag="PLI69_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG41141.1"
                     /translation="MNLHLFIARRIGTEGSGRGRMGIISNRIGCISVAVSILIMILAV
                     AIVAGFKSEIRSKATGFMGSVTLVAPGESPINELYPLSDSFSYKKAILDLEFVRGVDG
                     VVYCSGMIKSEDDINGLYFKGVDSLYNLAFFENCLYEGELPDYSGRISNDVLISRRLA
                     SRLGCSIGDDLVTYFIKDDVKVRKFRICGLYDAQLEDLDMTLAIVDKRHAQRINGWNK
                     NQVSSFEIAIDPGADIKEANAEIEEILYVNSTDEDDAMFTMPLTRILNNLFDWLALLD
                     LNMLMVLLLMMVVAGFNMISAILIILFEKISMIGLLKALGMRNRNIRKIFLLKAGSIV
                     GKGMIWGNIIGLLICFLQWKFKIIKLNPTNYFVSFVPIKLS"
BASE COUNT         2905 a         2334 c         2727 g         2909 t
ORIGIN      
        1 ttgaacagga tcccggtgga gccgcgcatc cgtatgtttt tctgctttag gagcgttaac
       61 tgtaggtaga agagctcctc aaacttgagc ctctcttttg ctttgtcgag tatctgaagg
      121 tcggtaggga aatgtatgtt gaccagcgcc tgacgcagag atatgaagcg tttgcgtagc
      181 agcagagagc gtggaagggt ctcttcaatg ctctcaatca cctggttaat gagattgtac
      241 tggagcttgg cgaagacctt gttgcctata caggcacttc gcagcctctc agtacttgaa
      301 tagacaccaa taagggttcc aaccatcgcg agtttatcct cggtggccgg gtctatttcg
      361 gggtgaacca tattataggt ctggttgaat atggagggct tgccgaatac tatatattct
      421 gctcctattt tgagtctgcc ggagatccat ttaatcccct gaaagaaaac aagttcgata
      481 gtacccgtcc catcgtacag gtaagctatc agacgtttgg cacgcccttc gccgactgtg
      541 cggatatccc tgatgctgcc tctaagctga atataagcac tgctgctgtc aatttccctt
      601 atggaataaa atcttgttcg gtcaatatat ctgaatggaa atgtatagag catatcccta
      661 aaagtggaaa tgcccaactc tttttgcagc aatgcagccc tcttaggccc gacaccctgc
      721 aggaacttta tgtcactttc cagaatattg ttcacaactg caaatataca ttaattatgc
      781 gtcaaatttc cgtgcttttt agtaacttgc ggaaatttta gaaacctatg agtagaaaaa
      841 tagtagtggg gattacccaa ggagacagta acggcatcgg ttacgaggtg atcatcaaag
      901 ctctttcaga tgcaaggatg ctcgacatgt gcattcccgt tgtatacggt tgctcacgca
      961 ctttcggtat gtaccgaaaa ctagtaccgg aaaccgaaca gattactaca aatataatca
     1021 acagtgcttc ggaagcacgt cccaaacatg tcaacatagt caatgccgtg ccggattcat
     1081 tggccgtaga tgtaggtcag cctacatttg atggagccag agctgccatt atagctctta
     1141 aggctgccgt agccgatgta aaagcaaatt tccttgacgc catagtcaca gctcccttca
     1201 gtaaacatac gatagcacag acggaattct ccttccccgg ccatacggag tacctgataa
     1261 acgaatttgg cgttgaagat ggtctgatgt ttttatgtgc cgataaccta agggtaggag
     1321 tagttaccaa ccatcttcct ctatcgaagg tcagctctgc aattacaaaa gaggttctta
     1381 tgagcaagat ccgtcttatg aaccactctc tcataaggga ttttggtata gtgaggccga
     1441 aaatagctgt tctgggactc aatccccatg caggtgacgg aggttcgctg ggtactgagg
     1501 agattgatac cattatccct gcaataaggg agtccaatgt agagaatata cttgccttcg
     1561 gtccctattc tccggatggt tttttcgcaa caaatacgca gtacaaattc gatgctgttc
     1621 tggctatgta tcatgaccag ggactcatcc ctttcaaggc gcttgccttc gataagggag
     1681 taaactttac ggcggggctg ccgattgtca gaacatcccc cgatcatggt acggcatttg
     1741 aaattgccgg taagtccatg gcaaatccgg gaccgatgat ttcggcaata tatacggcca
     1801 tagatatctg caacaaccgg gacaactatg atgagatgag tgccaaccct ctggaaataa
     1861 aacattttga ggggcctaag cacgaacgca cgatattgcc ggaataactg atggcaaaaa
     1921 gaacacccat acaaatatat acggatggag cctgcagagg caatcccggc cccgggggat
     1981 atggtgtagt gatgcgttgc ggaaattatg agaaggaatt ttcggcgggg taccgcctca
     2041 ctaccaacaa tcgtatggaa cttttggctg tcattatcgg cctggaggct atcaagtggt
     2101 cagatgccgc agtcactgtt tggagtgatt cctcatacgt agttaacgct gtggagaatg
     2161 gttcggtatt caactgggaa aagaaaaatt ttctaaaaaa ggccaatccc gatctttgga
     2221 tccgtttcct gaaagtttat agagagcaca aggtgagttt tgtgtggatt aaaggccact
     2281 ccgggcatcc tgacaatgaa cgttgcgatt accttgccgt cacagcctct atgggacctg
     2341 acctggccat ggacgaggtc tatgaaacat cgaatgttaa aaacgaactt tttaataatc
     2401 ttaaataatc atctatgaag aagtattttg ctgaagtaat aggcacgatg gtgcttgttc
     2461 ttctaggttg tggtagcgca gtcttcaatg gcggcgtagg caccactgca caggttttga
     2521 ctgtttcctt cgcattcggt ctttcagttg tggcaatggc ttatgctatc ggctctattt
     2581 cggggtgcca tataaaccct gccattaccc tcggtgttat gcttaccggt agaatgaaaa
     2641 caggcgaagg cctgaaatat atgcttgctc aggttatcgg tgcatttata ggttcggctc
     2701 tcatttttgc acttgtacag acaggagaca aggatgcagc catgtatgcc acaacaacag
     2761 gagcaaatgc tgtttcaaat tcagcctcgg tcggatcagc gcttattgcc gaaatggtgg
     2821 gtacatttat cttcgtttta gtagttctcg gtgttactga ttcaaagaaa ggcaatcccg
     2881 ctcttgcagg cctggccatc ggtttgacac ttgtgctcat tcacattgtt ctgattccaa
     2941 ttacaggaac atcggtaaat cctgcccgta gtattgctcc ggcagtattc gaaggcggcc
     3001 aggcactgaa ggatctctgg attttcatcg taggtccttt tgtgggtgct gcactttcgg
     3061 cagtggtttg ggatggactt gcaaaggaaa cggcaaagta acttattgct tccaaagtaa
     3121 atttaaagtg gggtgactaa aagtcaagca gacttcaagt tcaccccttt tgatattatt
     3181 atttaagcaa aactctatcc cagaacatta aacgcaactc gcaacttaat tacttgatat
     3241 tccatccaaa agagttaaat ttgtagatta ttaaatttac tttttagaga tgaagcacaa
     3301 tggacttaca ttgggagttc tgggaggtat ggggcctgct gcaacagcgg agtttttgag
     3361 actccttgct gaaaaggcac ccgcggatgt tgatcaggaa catccccgaa tgatagttta
     3421 ttcacatacg attactccgg acaggacaac ttttattctc ggcaaagggc cggatccgca
     3481 caattatatc agggaaggac ttgatactct catcggttgg ggtgctgacc ttcttgcagt
     3541 cacctgcaat accgcccatt acttcataga taagttcaga gatgaacttc ccgttcccgt
     3601 aatccacata atagaggaga ccataaaaaa atcgcaggcc gtatgtcccg agggtgcctg
     3661 gctcaccgct actctcggta ccatgcgtac cggtctctat cagaaacact cggcttactt
     3721 tggttatgat ttcaagctcc cttcagagga gatgcaggta gaaatccacg acataacaga
     3781 ccaggttaag gccggcaaac atcgtgaggc cggtgtcaga ttccgaaaag ttgtagaaca
     3841 actctgggaa atcgaaaaac ttcccgtagt gggtgcctgt accgagattc caatcgctta
     3901 cagtcatacc ggactgcctg aggaaatggg tatttcctgc ctggaggctc ttgcagaggg
     3961 atgcataagg gagctttacc gaccgctctg attttgtttg aaattgcatt gaaccagatc
     4021 tttaagatta gaaataatga gaagaatagt aaaatataca gtcttaatgg cgattatgct
     4081 gatggcagcc tcttgtggcg atcccaaacc ctctacgggt cctgacaatc catcaaaagt
     4141 gaattttgca aagggtgcgg atgtgagctg ggtcacacaa atggaaaggg atggtgtaaa
     4201 attctacaac tcaaagggta ctgagatgga gtgcatggcc ttgctcaaaa gtttgggcat
     4261 gaatactata cgactgcgag tatgggtcaa tccggttgat ggttggaatg gcaaggctga
     4321 tcttctggtt aaggcaaaaa gagccaaggc cctcggaatg cgccttatga ttgatttcca
     4381 ctatagcgac agttgggctg accccggaaa gcaaaataaa cctgcagcct gggttaatct
     4441 tccttttgag gagctcaaag gtgctttggt atcccatacc atagagatac tggaagcact
     4501 caaaggagaa agcatctatc ccgaatgggt acaggtaggt aatgagactt ctgacggtat
     4561 gctttgggag gatggtcgag cctcagtcaa tatgaaacaa tacactgatc ttcacaatgc
     4621 aggctacaga gctgttaagg aggttttccc tgaggctaaa gtgatacttc atattgatag
     4681 aggagatagc ggtgcacatt acaactggct tctcgacggt atggcaccct acttacctca
     4741 gtatgacata atcggaatgt cgctctaccc tgatccggga aactggcaat acatcaatga
     4801 ggcgtgtctg atcaacatga aagaactcat aagtaaatac aataaggata taatgatctg
     4861 tgaagtaggt atgagttggg acaagcctga aatctgcaag gggttcctta tagacctgat
     4921 agacaaagcg cttgaaaaga cggacgggcg ctgtctgggc gttctgtatt gggaacctca
     4981 gggatttgtc aattggagtc gctataccaa ggctgcattn nnnnnnnnng ccaactgtgg
     5041 ctctggatgc ctttaaggag tgatactgac caaattttta aaatatgaag aaatatattc
     5101 ccctggcatt actgctgagt atttttgctg ccgtatcctg ccataaagga aatccgcgaa
     5161 gcgagattct gctggaagag ggatggaagt tttccatcgg agatttcgat acggcatcgg
     5221 atcccgcttt tgacgactct gagtggcaag aggtacgcat tccgcacgat tgggcaatat
     5281 atggcccttt tgagaggtca aatgacctcc aggttgtagc agtgagacag aatgacgaaa
     5341 ttattgcaac agaaataacc ggtcgcaccg gcggacttcc ttatgtgggg gtggggtggt
     5401 atcgcaccga gtttgacctg cgtggatttg atggagagaa ggaggccgtc tctttgctct
     5461 tcgatggggc tatgagcggc gcacaggttt gggtcaatgg aacaaaagta attacttggc
     5521 cactgggcta taactctttt cattgtgaca tcactcaatg gatcaacgaa gatggggagg
     5581 acaactccat agcagtgcgt ctggaaaatc tcccaaaatc ttcgagatgg tacccgggtg
     5641 caggacttta ccgcaatgtt cgtctggtca ggacaagcaa acttcatatc cctatatggg
     5701 gaacttatat caccactccg gaggtttccc gtaagaaagc ggaggtctcc atgcagatcg
     5761 ccgtggaagg cagcactgac aaagaattga ggattgtcac tgaaatacgc aatcctaaag
     5821 gcaaaaaagt agcatattcc gagagtttgg ttgaagaatt gaacgatcag accgataccg
     5881 tcatgttgag gtttaccgtt cgcagacccg acctctggtc tcccgagtcg ccggctcttt
     5941 atacagctca tacaactatc tatgagggag atcttcttac cgacagccac gttacacgtt
     6001 ttggcatccg ttataccgaa gtagtagagg gcaaaggatt ttatctcaat ggtgagtcgc
     6061 gtaaattcaa gggtgtatgc aatcatcacg accttggtcc tcttggtgct gctgtaaaca
     6121 aggctgcgct ccggcatcaa ctggaaatgc taaaggacat gggttgtgat gctatccgta
     6181 cctcacacaa tatgcctgcc ccggaactag ttgacctttg tgacgaaatg ggattcatgt
     6241 tgatagttga ggctttcgat gaatgggata ttgccaagtg caccaacggc taccatctct
     6301 acttcgagga gtgggcagaa aaggatatgg tcaatatgat ccgtaatttc cggaacaatc
     6361 cgagtgtagt tatgtggagc atcggtaacg aggttcccac tcaatgtgat gatcagggtt
     6421 acaaggtggc cgcatttctt cagagtatct gtcacagaga ggatccttcg cgtcttgtca
     6481 cctgcggtat ggaccaggtg cagtgtgttt tggccaatgg atttgctcag cttatggatg
     6541 tcgtgggact caattaccgc acccatattt acaaggaggc attcgaaacc ctgccgcaag
     6601 gcaatatcct gggttcggaa acttcctcta cggttagttc caggggtgtt tataagtttc
     6661 cggttgctct gaaggcaggt gctaaatatg atgatcacca atcctcctct tacgatgtag
     6721 agtattgcag ttggtcgaat atccctgacg tcgattttgc ccttgccgag gattttgagt
     6781 ggacaatggg acaatttgcc tggacagggt ttgactatct gggtgagcct actccatacg
     6841 ataccgactc ctggcccaat catagttcac ttttcggtat aatagatctg gcatcgatac
     6901 ccaaagacag atattacctc taccgttcac aatggaatga aaatgataat acgcttcatc
     6961 tattgcccca ctggaactgg gaggggcgtg aaggtgaaac catacctgta atgtgttata
     7021 cggactatcc atccgccgaa ctctttgtaa atggcgtttc acaaggcgtt gtaacaaaag
     7081 gcagctattc tcttgctgat attgatcttt cgaaaatgga caaagatgcc ttgatggcca
     7141 gatacagact tatttggccg gatgtggtct atcagcccgg agagatagag gtagtggctt
     7201 acaatgccaa ggggcgtgag cagatgcgta aacgtatcgt aactgccggc gaaccccatg
     7261 cgattgtgct cgaagccaat cgcaataaga ttgaagcaga tggtagggat ctggcttatg
     7321 tttatgtcag tatagttgac aaggatggga atctatgtcc aaatgcctcc aatgaggtaa
     7381 ggttcgaagt gaagggagcc ggagaatttg tagcggtggc aaatggtgat cccacctgcc
     7441 tctatccatt ccacgagccg ataattcccg ctttcagcgg tcgtttgacg gctatagtca
     7501 cttcgcacga ctatcgcggt agtatgtccg tgaaagcctt ttcagagggg ctcgaaccgg
     7561 cacaaatcat aatcaagaca aattaaagca gacaataatg atagattcaa aaattcttaa
     7621 gaaagcgata aaggataaat tcggtcattc cgatttttca ctcttctcag ctccgggacg
     7681 tatcaatctc ataggtgagc ataccgatta taatggcggc ttcgtactgc ctgcatccat
     7741 agacaaatgc atttccctgg caatagtacc taatggtacc tctaccgtaa atctctattc
     7801 catagattac gatgagagtg tttcattcga tgttgacggt gcaaagcctg agcagcagtg
     7861 ggcatcctat gtatatggtg tgattcagga gatgaagaaa aggggcgctt cacccaaagg
     7921 ttttgatgcc gtatttggcg gagatattcc tctcggagcg ggtatgtcct cttcggcagc
     7981 cctcgagtcc gtttttggaa cggctctcaa taccattttc aagctgggat ttgataaaag
     8041 ggaattggca aagatcggtc agatgacgga acacaattat gtcggtgtcc gctgcggcat
     8101 aatggatcaa tttgcctccc tttttggaga agccggaaag gtaataaggc ttgactgccg
     8161 ttcgctggag tatgaacttt tccctttcgc gcccgaaggc atcagactgg tgcttataga
     8221 tacgatggtc aaacattcgc ttgcctcttc ggagtacaat gttcgtcgtg cccagtgtga
     8281 ggcgggtgtc gccgtcatat ccaggtacaa ggaaggagtg gagctgctca gagatgtgac
     8341 catggaggat cttgatgctg acaaggggga aatggatccc gaggtctatc gcagatgcca
     8401 ttttgtgata agtgagaacc agcgtctttt ggatggttgt gaggatttgt cgaggggtga
     8461 ttatgctctg tttggtcaga agatgttccg tacccacgaa ggtttgagca aagagtatgg
     8521 ggtaagttgc gaggaactcg actttatcgt agagatagca cgtggtgtgg agggagtttt
     8581 gggagccaga atgatgggtg gcggcttcgg cggctgcgtt ctcgcaatgg tgaaaaatga
     8641 gaaccatgat gagtatgttg caaaagtgcg cgaggcttat cagaagcgct tcggcaagga
     8701 tccgcgcgtt attgatgtgg ttatttcaga tggagcaagg caatggtaga ttctgacatc
     8761 aaatatttta ctctcaccaa cagtagcggt gcgagtgtca tcctcagcaa tctgggtgcg
     8821 ggtattattt cggtcatcgt acccgacaag gatggcaata tggctgatgt agccctgggt
     8881 tatgcaaagc ctgaggatta tttttgtgat ggtccctgta tgggaaaggt accgggaaga
     8941 tatgcaaaca ggatagcctt cggcaaattt tccctttcgg ggaaagaata ttccctttca
     9001 ataaacaacg gtcccaacca tctacacgga gggccgggag aggagtgctg ggcaaaccgt
     9061 atctggtttg gcgtgcagaa tggcaatgaa gtctcctttg cccttgaaag tggtgacggt
     9121 gatgccggat accccggaaa tgtaatggct gaagtcatct acaggtggga cgaagagtct
     9181 cgtctgacca tcacttttcg cgctaagagc gatgccgata cgattatcaa tctcaccaac
     9241 cacgcctatt ttaacctaaa aggcgagggc aggggagata tcaaagatca cactttgcaa
     9301 ctcgaagcat ccaattatct tccaactgat gaaaccctaa tacctgccgg ttcaccggct
     9361 ccggtggaag gcactccgat ggatttcagg actccggtca agttagggga gcaccttttt
     9421 gacgattttc cggcacttcg ttacggcaaa ggatatgaca actgctgggc ggtggacgga
     9481 tggcagaaag gagtagtgcg caaagtggcc gaactcgcaa atcctgccag cggccgaaag
     9541 ctcatagtaa gcaccgatgc gcccggtatt cagatttaca ccggtaactg gctcaagggt
     9601 agccctaaat ccaaaagcgg agcggagtac gatgactatt acggagtggc actcgagtgc
     9661 cagtctttcc ccgattctcc taaccgcccc gatttccctt caaccttact ggctgctgga
     9721 gatacctggc aaagagtgat tgaatttcat tttaccaaat gaatctccac cttttcatag
     9781 ctcgcaggat aggtaccgaa ggctccggca gaggtagaat gggtatcatc agcaaccgta
     9841 ttggctgcat atccgtagct gtcagcattc taattatgat attggcggtg gccatcgttg
     9901 cgggatttaa atcggaaata cgatcaaaag ccacgggttt tatgggttcg gtaacacttg
     9961 tggctccggg tgaatctcct attaacgaac tttatcctct gagtgactct ttttcctaca
    10021 agaaagcaat ccttgatctg gagttcgtca ggggagtaga cggagtagtt tattgctccg
    10081 ggatgataaa gagtgaagat gatatcaacg ggctctattt taagggggtg gattcactct
    10141 ataatcttgc tttttttgag aattgtcttt atgaaggaga attgcctgat tacagcggaa
    10201 gaatctcaaa tgatgtgctt atctctcgcc gtcttgcctc ccgtctcggt tgctctatag
    10261 gagatgattt agttacttat ttcataaaag atgatgtcaa agtgaggaaa ttccgcatct
    10321 gcggcctcta tgatgcacag cttgaggacc ttgacatgac tctcgcaatt gtcgataaac
    10381 gccatgcaca acgcatcaac gggtggaata aaaatcaggt ttcctcattt gagatagcaa
    10441 tagatccggg ggccgatatc aaagaggcaa atgccgaaat cgaagagatt ctttatgtca
    10501 actctaccga tgaagatgat gcaatgttta caatgccgct cactcgcatt ttaaacaatc
    10561 tttttgactg gctggccctg ctcgacctca atatgctgat ggtattgctg ctgatgatgg
    10621 ttgtggcagg tttcaacatg atatctgcta tactgattat tctttttgaa aagatatcga
    10681 tgattggttt gctcaaagcc ctcggaatgc gcaatcgaaa tatcaggaaa atattcttac
    10741 tgaaagcggg atcaattgtg ggcaaaggaa tgatttgggg taatattatt ggactcctta
    10801 tctgtttcct ccaatggaaa ttcaaaatca tcaagctcaa tcccaccaat tatttcgtaa
    10861 gttttgtgcc tataaagctc tcttt
//
LOCUS       DAOSIE010000017        10741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_6030_length_10741_cov_3.126614, whole genome shotgun sequence.
ACCESSION   DAOSIE010000017 DAOSIE010000000
VERSION     DAOSIE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6030_length_10741_cov_3.126614"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLI69_01200"
     CDS             <1..271
                     /locus_tag="PLI69_01200"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOG41142.1"
                     /translation="GITYDEVDYYLLHQANIRIIETIRKTLDQDKVKFPHNIENYGNT
                     SSASLPILLDELNRNQTLNSGDTIVLSAFGAGFTAGACLLKWSKN"
     gene            277..1221
                     /gene="fabK"
                     /locus_tag="PLI69_01205"
     CDS             277..1221
                     /gene="fabK"
                     /locus_tag="PLI69_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003566773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HOG41143.1"
                     /translation="MIKTRITELLGIEYPIVQGGMAWVADASLASAVSNAGGLGLISS
                     INAGADVVLEEIRKCKKMTNKPFGVNIMLMSPIADQVAQFVIDEQVPIVSTGAGTPAK
                     YMEQWLKAGIKVIPVVASAAFAIRMEKIGAVAVVAEGGEAGGHIGEMNTMALIPQVCD
                     AVSIPVIAAGGIADGRGFAAAMMLGAEGVQIGTRFITATESNAHDNYKQKIIAASDTD
                     TIVTGKTLGHPVRALKTPFSRKFAQMENDPTQTPESIMEFGSGSLRKAVKDGDEKMGS
                     FMAGQGSGLVKKVQPAKEIILEIVNDARNLLEGSSKYF"
     gene            1223..2467
                     /gene="fabF"
                     /locus_tag="PLI69_01210"
     CDS             1223..2467
                     /gene="fabF"
                     /locus_tag="PLI69_01210"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOG41144.1"
                     /translation="MARRVVVTGMGVISPVGNNLQEFWSSIIAGKCGIGMIERFPTDN
                     LLVKIAGEVKNFNPGDHGIDPPTARKNDRYALFALAAAHQAMTASGLNKENIAPERLG
                     VYIGSGIGGMDTFVTETTKLVQDGPKWVSPLFIPMMISNIASGNIAIEYDARGVCLPI
                     VTACATGTHSIGEAFRAVKHGYADAIICGGSEAAVHPLSISGFANSRALSRSEDPLQA
                     SLPFDIRRQGFVIAEGAGIVVLEEYNHAVARGAKIYAEVSGYGNTCDAYHYTAPRPDA
                     TTAAEAMRQALEEAGYRKGEKIHINAHGTGTPLNDKSETLSIKLAVGEEEARKVAICS
                     TKSMTGHMLGGAGGVELVAAVMALSEGIIPPTIGLNEPDPECDLDYTPNKARNFAADI
                     ALSNSLGFGGHNGCIALRTINL"
     gene            2495..2935
                     /gene="fabZ"
                     /locus_tag="PLI69_01215"
     CDS             2495..2935
                     /gene="fabZ"
                     /locus_tag="PLI69_01215"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOG41145.1"
                     /translation="MTREQIKEFIPHREPMILVDKIEIDDNNVAHASYYVTGDEFFLK
                     GHFPGHPVVPGVILCEMMAQACSLLIGDDLKGRTPFYAGIEGVRFKNSVFPGDTFETT
                     ARIIDKKGMVYFTEATGSVDGKLCVKSKLAFALVDNDKLEERKQ"
     gene            2945..3685
                     /gene="fabG"
                     /locus_tag="PLI69_01220"
     CDS             2945..3685
                     /gene="fabG"
                     /locus_tag="PLI69_01220"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOG41146.1"
                     /translation="MGLLKGKIALVTGASRGIGKAIAIKFAQEGADIAFTARSETKHF
                     LDTVAQVTTFGVRCKGYTSDASDFKQTHELVEQIMADFGRIDILVNNAGVTRDGLIMR
                     MSEEQWDEILTVNLKSAFNFTHAVVPVMARQRGGSIINMTSIVGISGNAGQCNYAASK
                     AGMIGLAKSIAKEMGSRGIRANSIAPGFVETEMTDALPEDVRQGWLKMIALRRSATPE
                     DVANVALFLASDLSGYVTGQTIVVDGGM"
     gene            complement(3758..4816)
                     /locus_tag="PLI69_01225"
     CDS             complement(3758..4816)
                     /locus_tag="PLI69_01225"
                     /inference="COORDINATES: protein motif:HMM:NF026250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6359 domain-containing protein"
                     /protein_id="HOG41147.1"
                     /translation="MKRFVLMGVLFSIIMASCNMDAFSEGEASLTFSFAVPLKTTVTK
                     ALSMPDTNSFVLLVRSNTGETLYNGLYGARPTEITVAAGTYEVSVQSVEFEKPAFETP
                     QFGDSKVIVVSNGEKVSVAFLCKLLNSGMRFSFTERFLQKHNSGHILLEQEAGNLEYL
                     FTEQRTAYLKAGNVGICHKMTTASVPLFNRTLGPGEIYNITLDASTAESASEFSIKVD
                     TTALYINEKVIIGEEFSGADGSSAEKAVSVSQAKNRVGDTLWVWGYIVGGDMTSSSVT
                     FVGPFTKDSHIAIAENPSEKNREACFSVELSKTAVKSALNLVSNPTNLGRKVFLKGAV
                     VESYFGLNGLKKVTEYSM"
     gene            4963..8247
                     /locus_tag="PLI69_01230"
     CDS             4963..8247
                     /locus_tag="PLI69_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG41148.1"
                     /translation="MYKITNHPILDVPQEDLVEFLFEGTPVRGQKGHTIGAALHQAGF
                     PVHQHSLLGRHRSMECGIGKCGACEMLVDGTIRRICITKVDGVKVVERIGDANSAGGK
                     GVLAAVPESFAKDAPKRTYRTTVAIIGAGPAGLAVREELNKAGVDNIVIDNNDKIGGQ
                     FVMQTHQFFFFERERKFGGMRGFDIAKTLSGEDEEGIFLNSTVWDILEGKLIAVKNIV
                     TQEVFYVEADQLVIATGALPFMPALKNDDVPGVYTAAVVQRMMNKEYTLLGKNILTVG
                     AGNIGYLTSYQAMQAGARVKAIIEAMPHEGGFPVQANRVRRLGIPIMTSHILLEAIPN
                     EDRSSITGAIIARCENFKAIPGTERRIEGIDCINICTGLMPDNQLLRKGQEVFGLACH
                     GVGDAVRIGEGTSAVLRGRQCAFEIMQALGRRINYDEYLNISKEYIDSQQHPKRILDE
                     PRIPSAERIAKRGFVLIDCLYGFACNPCSFACRYGAIAKSSTSTVPMIDYDKCIGCME
                     CVSQCPGLAIFGYNTGRRQLYLPIEYAAESGNEVFLVDNDGKKVGEGIIERILIKPNR
                     TNVAIVKATEIYGESSPGDLLKARGFILKEEYPEMPDLQPVSKTESAREDSADTYICH
                     CEDVTLERLLEMMGNRTSITAQELKHITRIGMGVCRGSRCLQRTRQVLRSHGIEVIGE
                     FTPRGPMANLVEIGAVSNREGSGVFITSDRFTDQTPIKVGAFVAGGGIAGSATFRYLA
                     ESGFAPILINMDHGSSWRNIAGGRPAFSLPALADIAKNNLEIFKDLHSKGNIDYRLIR
                     YISFAHDEATYKALEASTAWSDAYMVEKKDFKREISDYFNPNLDTYSHALITKDCWQA
                     SPGRTIDLVRHLGIEAGGTLFENTQLVSVSKNGDLYELLVKHPDGKYVRYQSEIFVNA
                     LGANAEKFAKQLGIETGLFPVRHQAFITKRLPLLGKNGAPLDMLIDRRKYKGFSAVYG
                     QQLADTGQIIGCASPANDATDTFRNLKVGTREFIEIVAEVFSDWIPQLRGMSFQAMWS
                     GYYIEPRYIVDPARGLFMGMRGHGFMLSQYIAKLYVDSLLGREVPDYFYKLALDGPGL
                     SEQAFK"
     gene            complement(8302..9522)
                     /locus_tag="PLI69_01235"
     CDS             complement(8302..9522)
                     /locus_tag="PLI69_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOG41149.1"
                     /translation="MKLSKALCRLIFGLTFILSGFLKLTDPIGTGLIIQEYLTVFSMR
                     FLDFASIGLGIALSAIEFVIGISILLGLRMRFFTWMGLGFISFFTLLTLYLAIFNPIT
                     DCGCFGEAIHLTNWETFYKNLVLLTCALVLFFNRKSFIPIAYAAFEWVSVGLFLGIAL
                     LIAIPTYYNIPQIDFTAYKIGTDLDMLTRESHAEYETIFLYSKDGKQQEFGLNDLPDS
                     TWTFVDSRTELISGSPELAQVDISLRDTAGEYRTEVLLQEGPLVAGVIWDPEEMTPQM
                     WTNLVILKSETLSEGSRFILLSSVREFDGNQAEAFFPQEIIYTADRKALMTLVRSNGG
                     GVYFFDGTIIHKWPSRRITSERISAILSEDSDQVVLNDSIHQRLYINILLISIILIVI
                     LMRYFCKSFYTHKE"
     gene            complement(9519..10196)
                     /locus_tag="PLI69_01240"
     CDS             complement(9519..10196)
                     /locus_tag="PLI69_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HOG41150.1"
                     /translation="MLIVLEGLDGAGKSTQLKMVSNYMESRGHKVEYLHFPRFDAPVF
                     GDLIAGFLRGDYGTIDEVHPQLVALLFAEDRRDAAPMLRKWLDEGRCVVLDRYFYSNI
                     AFQCSKLESDEESRRLRDWILDVELGHFAIPVPDLNLFLDVPIKFVDSKLNTSRQGDD
                     RAYLEGKNDIHEADISFQVKVRDIYLEQCALDRSFKRIDCSDIYGNMLPANDIFIKIR
                     TEMEKLS"
     gene            10249..>10741
                     /locus_tag="PLI69_01245"
     CDS             10249..>10741
                     /locus_tag="PLI69_01245"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOG41151.1"
                     /translation="MEKQRVEIMGIVNVNDDSFFAQSRAKEKDAFIARVEMMLNQGAD
                     SIDIGAVSTRPNMASVSLEEEWERLSPVVQLFASRFKGVRFSVDTYRGEIVKRVYEAA
                     GPFVVNDISAGEEDPQMLDRVAEFDLEYIAMHRKGDCVTMHHKYEYEDVVGEVVEYFR
                     KFDE"
BASE COUNT         2906 a         2359 c         2683 g         2793 t
ORIGIN      
        1 cggcatcact tacgatgaag tggattacta tcttctccac caggcaaata tccgtattat
       61 tgaaacaata agaaaaacgc tcgaccagga caaagtcaaa tttccgcaca atatcgagaa
      121 ttatggaaac acatcctctg ccagtttacc cattctcctt gatgaactaa accgtaacca
      181 aacactaaat agcggagata ccatagtgtt gagcgcattt ggagcaggct tcacagccgg
      241 ggcctgtctc cttaaatggt caaaaaacta ataatcatga taaaaacaag aattacagaa
      301 ctgttgggta ttgagtaccc tatcgtacaa ggtggtatgg cttgggtagc cgatgccagt
      361 ctcgccagtg ccgtctccaa tgcaggaggc ctgggtctca tctccagcat caatgcaggt
      421 gcggatgtgg tccttgaaga gatccgaaaa tgtaaaaaaa tgacaaacaa gcctttcggc
      481 gtaaacataa tgctgatgag tcccattgcg gatcaagtgg cacagtttgt tatagatgaa
      541 caggtaccga tagtttccac aggtgccggt acgcctgcaa aatatatgga acagtggctt
      601 aaggcaggta tcaaagtaat acctgtcgtg gcctcagctg cttttgccat acgaatggag
      661 aagataggtg cagtcgctgt tgttgctgaa ggcggtgaag caggagggca tattggtgag
      721 atgaatacca tggcccttat accgcaggtg tgcgatgcgg tttccatccc cgtaattgct
      781 gcaggtggta ttgcagatgg cagaggcttt gccgctgcga tgatgctcgg tgcagaaggt
      841 gttcaaatag gcacccgctt tattaccgcc actgaatcta acgctcacga caattataag
      901 caaaaaatta ttgccgcaag cgataccgat accattgtta ccggtaaaac tcttgggcat
      961 ccggtacgag cattgaaaac tccatttagc agaaaatttg ctcagatgga gaacgaccct
     1021 acgcagaccc cggaatccat aatggaattt ggtagcgggt cgctccgtaa agcggttaag
     1081 gatggagatg aaaagatggg cagcttcatg gccggccagg gttcaggact ggttaagaag
     1141 gttcaacccg caaaagagat tatactggag atcgtaaacg atgccaggaa tcttctcgaa
     1201 ggttcttcta aatattttta atatggcacg tagagtagtt gtaacaggaa tgggggtaat
     1261 atcccctgtt ggaaataact tgcaagagtt ctggtcttcc attattgcag gaaagtgcgg
     1321 aatagggatg attgagcgtt ttcctacgga caatcttttg gtaaaaattg ccggtgaggt
     1381 caaaaacttc aaccccggtg atcatggcat tgacccacct actgcacgaa agaatgatcg
     1441 ttatgcactc tttgctcttg ctgcggccca ccaggccatg actgcaagtg gtctgaataa
     1501 ggagaatatt gctcctgaac gcctcggagt ttatatagga tcaggtatcg gaggtatgga
     1561 taccttcgtt acagagacca ccaaactggt tcaggatggt ccaaagtggg tatctccact
     1621 atttatcccg atgatgatat ccaatattgc ttccggcaat atagccatcg agtacgatgc
     1681 gaggggtgtt tgtctgccta ttgttactgc ttgtgccacg ggtacccact cgataggaga
     1741 ggcctttagg gctgtaaaac atggttatgc tgacgctatc atctgcggag gttcagaagc
     1801 tgctgttcac ccgctttcca tcagcggatt tgctaatagc agagcattat cccgctcgga
     1861 ggatcctctg caagcctctt tgccttttga catacgcagg cagggtttcg ttatagctga
     1921 gggagcagga atagtggtgc tggaggagta caaccacgca gtagctcgcg gcgctaaaat
     1981 ctatgcggag gtcagtggct atggcaacac ctgtgatgct taccattata ctgcaccgcg
     2041 cccagatgct acaacagcag ctgaggcaat gcgccaggca cttgaagagg ccggataccg
     2101 taagggagag aaaatccata tcaatgctca cggtacagga acccctctca acgacaagtc
     2161 cgagacactc tcaataaaac ttgccgtagg tgaagaggag gctcgcaaag ttgccatttg
     2221 ttctaccaag tccatgaccg gtcatatgct gggtggtgca ggaggtgtgg agctggtagc
     2281 agccgtgatg gctctcagtg aggggattat ccctcctacg ataggtctga atgagccgga
     2341 tcccgagtgt gacctcgatt atactccaaa caaggcacgc aattttgccg cagatatagc
     2401 tctctccaat tcactgggat ttggtgggca taatggctgt atcgctttgc gcacaatcaa
     2461 tttataaacc ttttaattat atttgcacag gattatgact agagagcaga tcaaagagtt
     2521 catccctcac agagagccga tgatactggt tgataaaatc gaaatcgatg acaataatgt
     2581 agcacatgcc agttattacg tcactggaga tgaatttttc cttaagggac actttcccgg
     2641 ccatccggtt gtacccggag taatactttg cgagatgatg gcacaagcct gctcgttgct
     2701 aataggtgat gatttgaagg gacgaacacc tttttatgca ggtattgaag gagtaaggtt
     2761 caagaattcg gttttccccg gagacacttt tgagactact gccaggatta tagataaaaa
     2821 agggatggtc tattttacag aagctaccgg gtctgtagat gggaaactgt gcgtgaaaag
     2881 caaactggca tttgctcttg tggacaacga caaattggag gagagaaaac aataattaag
     2941 agatatggga cttctaaagg gaaaaatcgc actggtaacc ggtgcatcga gaggtatcgg
     3001 caaagccatt gccattaaat ttgctcagga gggtgcagat atagcattta cggcacgtag
     3061 cgagactaag cactttctcg ataccgtggc gcaagtaacg acatttggag tgcgatgcaa
     3121 aggatatact tcagatgcat ccgattttaa gcagacgcac gagcttgtag agcagataat
     3181 ggccgatttt ggcagaatag acattttggt caacaatgca ggtgtgactc gtgacggcct
     3241 tataatgaga atgagcgaag agcagtggga tgaaattctt acagtcaatc tcaaatcggc
     3301 attcaacttt acccatgccg tagtgcctgt tatggcgcgc caaagaggtg gcagtattat
     3361 caacatgacc tctatcgtag gtatttccgg caacgcggga caatgcaact atgcagcttc
     3421 aaaagcagga atgatagggt tggcaaagag tattgccaag gagatgggtt cacgtggtat
     3481 tcgtgccaat tccatagctc cgggctttgt ggagaccgaa atgaccgatg cccttcctga
     3541 agatgtacgc cagggatggc tcaagatgat tgcgctccgt cgcagcgcca ctcccgaaga
     3601 tgtagcaaat gtcgctctct tccttgcctc cgacctttcc gggtacgtca caggccagac
     3661 gatagtagtg gatggaggaa tgtaaaaaaa tgttcggaaa tttccgaaca tttttttagt
     3721 gtttagttcc gagttccgag taactccata ctccaaacta catactatac tctgttactt
     3781 tcttcaggcc gttgaggccg aaataggatt ccacaaccgc tcccttaaga aaaacttttc
     3841 gtccaaggtt agtgggattt gagaccagat ttagggcact tttaacggct gttttagaga
     3901 gctctacaga aaagcaggct tcacgattct tttcggaggg attttccgca atggctatgt
     3961 gcgaatcttt ggtaaagggg ccgacaaagg taaccgatga tgaagtcatg tcacctccca
     4021 cgatgtatcc ccatacccac agtgtgtcac cgacacgatt tttggcttga gataccgata
     4081 ctgccttctc tgcactgctg ccgtctgccc cgctaaactc ttcacctatg attacttttt
     4141 catttatgta caaagcggta gtgtctactt taatgctaaa ctccgaggca gattctgccg
     4201 tcgatgcatc caatgtgatg ttgtagatct cacccggccc aagagttcgg ttgaacagcg
     4261 gtacgctagc cgttgtcatc ttgtggcaga tgccgacatt gcctgccttg agataggcgg
     4321 tacgctgctc cgtaaacaga tactccagat tgccggcctc ctgctccagc aaaatgtgtc
     4381 cgctgttatg cttttggaga aatctttccg taaaagagaa tctcatacct gaattgagaa
     4441 gtttgcagag aaatgccaca gatacctttt caccatttga gacaacaata actttgctgt
     4501 caccaaactg gggagtctcg aatgcaggtt tttcaaattc gacagattgt acagagactt
     4561 cgtatgtacc tgcagctact gtgatttcag tcggtctggc tccataaagt ccattgtaga
     4621 gtgtctctcc tgtgttgctc ctgaccagca ggacaaatga gttggtgtcc ggcattgaca
     4681 gggctttcgt aacggtagtt ttcagtggca cggcaaaaga gaatgtgagt gatgcctcgc
     4741 cttcagaaaa ggcatccata ttgcaggatg ccataatgat ggagaataaa acccccatca
     4801 gcacaaatct tttcattgtt tacctccatt ttatctagtt taacactgtc gcaagatatc
     4861 tcctttactg caaacaaccc gtgttgaaac ggactatttt gaaaaagttt ctttttctgt
     4921 aaaaaaaagc taaatttggc aattcttaat gcacgcgatt ttatgtataa aataacaaat
     4981 caccccattt tggatgttcc acaggaagat cttgtggaat ttctatttga aggcactcct
     5041 gttcgcggac agaagggcca taccattgga gcagcgcttc accaggcggg atttcctgtt
     5101 caccagcaca gtcttttagg gcgccatcgt tctatggagt gcggtatcgg caaatgtggt
     5161 gcctgtgaaa tgttggtcga tggtacaatc cgcaggatat gtatcactaa ggttgatggt
     5221 gtcaaggtag ttgagcgtat tggggatgcc aattctgcag gaggtaaagg ggttttagca
     5281 gcagtgccgg agagctttgc taaagatgcg cctaaacgta cttatcgtac cacagtagct
     5341 ataattggag cgggtcctgc cggtctggct gtgcgcgaag agctcaacaa ggcaggggtg
     5401 gataatattg tcatagacaa taatgacaaa atcggcggtc agtttgtgat gcagactcac
     5461 caatttttct tcttcgagcg tgaacgcaag ttcggcggaa tgagaggttt cgatatagca
     5521 aaaactcttt caggagagga cgaggaagga atttttctaa attctactgt atgggacatt
     5581 cttgaaggca agctgattgc cgttaaaaac attgtgacac aggaggtgtt ctatgtagag
     5641 gctgaccaac ttgttatagc taccggagca ttgcctttta tgcctgcgct aaaaaatgat
     5701 gatgtgccgg gagtttatac cgccgccgtg gttcagagga tgatgaacaa ggagtatacc
     5761 cttctaggga aaaatattct caccgtaggt gccggtaata taggttatct cacttcctac
     5821 caggcaatgc aggccggtgc ccgtgtaaag gcaattattg aggcgatgcc tcacgaggga
     5881 ggatttcccg tccaggccaa tcgcgttcgc cgtctcggta ttccaataat gacttctcat
     5941 attttgcttg aggccatacc caatgaagat cgcagcagca tcaccggagc aattatagca
     6001 cgatgtgaaa atttcaaggc gattcccggt acggaacgcc gtatagaagg cattgactgc
     6061 ataaacattt gtacaggact tatgcctgat aaccagctgc tgcgcaaagg gcaggaggta
     6121 ttcgggcttg catgtcatgg agttggagat gcggtacgta taggagaggg tacttctgct
     6181 gtactaaggg gtcgccagtg tgcttttgag atcatgcagg cactcggtcg caggatcaat
     6241 tatgatgaat accttaacat ttccaaggaa tatatagact cccagcagca ccccaaacgg
     6301 attctagatg agccgcgtat tccctctgcc gagcgtatag caaaaagggg ttttgtcttg
     6361 atagactgtc tttacggatt tgcctgcaat ccctgctctt ttgcctgccg ctatggtgcc
     6421 atcgctaaga gttccacctc tacagttcct atgatagact atgacaagtg tattggctgc
     6481 atggagtgcg tatcacaatg tccgggactt gctattttcg gatataatac cggccgaaga
     6541 caactatatc ttcccataga gtatgccgcc gagagtggaa acgaagtttt tcttgtggac
     6601 aacgacggga aaaaggtggg agagggcata attgagcgta tcctgattaa gcctaaccgt
     6661 accaatgtag ccattgttaa ggctactgag atttacggtg agagctctcc cggggacttg
     6721 ctcaaggcac ggggatttat attaaaagag gagtatccgg agatgcctga ccttcagccg
     6781 gtatcgaaaa cagagtcggc cagagaggat tccgccgata cctatatttg tcattgtgaa
     6841 gatgttactc ttgagcggtt gctggaaatg atgggtaatc gcaccagtat cacagctcag
     6901 gagctaaagc atattacccg tataggtatg ggtgtatgca gaggcagcag gtgtcttcag
     6961 agaacccgtc aggtgctacg cagccatgga attgaggtta taggtgagtt tacccccaga
     7021 ggacctatgg caaacctggt cgagatcggt gccgtttcaa accgcgaggg tagcggtgtt
     7081 ttcatcacct cggatcgctt caccgatcag acacctatca aggtaggtgc tttcgtcgcc
     7141 ggaggcggaa tagccggcag tgccacattc agatacctgg ctgagtccgg ttttgcccct
     7201 atattgatca atatggatca cggcagctcc tggcgaaaca ttgccggtgg ccgtcctgcc
     7261 ttttcgctgc ctgcgcttgc ggatattgct aaaaacaatc ttgagatatt taaagatctc
     7321 cactccaaag gaaacataga ttacagactt ataagatata taagctttgc ccatgacgaa
     7381 gcaacttaca aggccttgga ggcctctacc gcctggtcgg atgcttatat ggtggagaaa
     7441 aaggatttca aacgagaaat ttcggactat ttcaatccga atctcgatac ttacagccat
     7501 gctcttataa cgaaggattg ttggcaggct tcccccggta ggaccattga tttggtgcgc
     7561 catcttggaa ttgaggccgg aggtacccta tttgaaaaca ctcaacttgt aagtgtttca
     7621 aaaaatggtg atctctacga attgcttgtc aagcatccgg atggcaaata tgtgaggtat
     7681 cagtcagaaa tatttgtcaa tgcgcttgga gcaaatgcgg agaaatttgc aaaacagctc
     7741 ggtattgaga caggattgtt ccctgtgcgt caccaggctt tcattaccaa gcgtctccct
     7801 ctgctgggca agaatggagc tcctctggat atgcttattg atcgtcgtaa atacaaggga
     7861 ttctcggcgg tttacggcca gcaactggca gatacgggcc agattatcgg atgtgcttca
     7921 ccggcaaatg atgcgaccga tacattcagg aatctgaaag tgggtaccag ggaatttatt
     7981 gagatagtgg ctgaggtctt ttcagattgg ataccgcaat tgcggggaat gagtttccag
     8041 gccatgtgga gcggttatta tatcgagccg cgctatatcg tggatcctgc cagaggactc
     8101 tttatgggta tgcgaggaca tggctttatg ctctcgcaat atatcgcaaa actctacgtc
     8161 gactcactcc ttgggcggga agtccccgat tatttctaca aacttgctct cgacggcccc
     8221 ggcctcagcg aacaagcctt taagtagtgt tgcgggcact cggtactcag aactccgcac
     8281 cctgcacccc gcaactcgca tctattcctt gtgtgtgtaa aaggatttgc agaagtagcg
     8341 catcaggatg actatgagaa ttatgctgat gagcagtatg ttgatataga ggcgctggtg
     8401 gatggagtcg ttaagtacca cctggtcgct atcttcagat agaatagcgg aaattctttc
     8461 tgaagtaatc cttcgcgaag gccatttgtg gattattgtt ccgtcaaaga aatagactcc
     8521 tcctccattg gagcgaacaa gtgtcatcag tgccttgcgg tcagctgtgt agatgatttc
     8581 ctgagggaaa aatgcctcag cctgattccc gtcaaactcc cttacgctac tcaaaagtat
     8641 aaaccggcta ccctctgata gtgtctcaga ctttagtatg actaaattag tccacatttg
     8701 cggcgtcatt tcctccggat cccaaatcac acccgctacc agcggaccct cttgcaagag
     8761 cacctcagta cggtactctc cggctgtatc cctaagtgaa atatccacct gtgccagttc
     8821 cggtgaacct gaaatcagct ccgtcctcga atctacaaag gtccaggtgg aatcgggcag
     8881 atcgttgagg ccgaactcct gttgcttgcc atccttggag tagagaaata tcgtctcata
     8941 ttcagcgtga gactctctgg taagcatatc aagatcggtt cctatcttgt aggcagtaaa
     9001 atctatctgg ggaatattgt aataggtcgg aattgcaatc agcagcgcaa tacccaggaa
     9061 gagtcccacg cttacccact caaaagcggc gtatgctatt gggataaagc ttttgcggtt
     9121 gaaaaaaagc accagcgcac aggttagcag cacgagattt ttgtagaatg tctcccaatt
     9181 ggtcaggtgt atggcttcgc cgaagcaccc gcaatctgtt atcgggttga atatggccag
     9241 atagagtgtg agcagtgtga aaaaagagat gaaaccaagc cccatccagg taaaaaatct
     9301 cattctcagt cccagtaata tggatatacc tataacaaac tctattgctg aaagtgcaat
     9361 acccagtcca attgaagcaa aatccagaaa tctcatagag aaaaccgtga ggtattcctg
     9421 aataatcaat cccgtaccga tgggatcggt aagcttgaga aaacctgaaa gtatgaatgt
     9481 gaggccgaat ataagccggc acagggcttt tgataacttc atgacaattt ctccatctcc
     9541 gttcgtattt taataaaaat gtcgtttgcc ggaagcatat tgccatatat gtcactacaa
     9601 tcgatcctct tgaaactcct gtccagagca cattgctcca gatatatatc cctcactttg
     9661 acctgaaatg agatgtcagc ctcgtgaatg tcatttttgc cctcaaggta ggcgcggtca
     9721 tcaccctggc gagaggtgtt aagtttcgaa tcgacaaact tgataggcac gtcgaggaac
     9781 aagttgagat ccggtaccgg aatggcgaaa tggcccagtt caacatcgag aatccaatcc
     9841 ctaaggcggc gagattcttc atccgattca agcttggagc actggaaggc tatgttggag
     9901 tagaaatatc tgtccaacac aacacatctt ccttcatcca gccatttgcg caacatgggg
     9961 gcagcatctc tgcggtcctc agcaaagagc agcgctacta gctggggatg aacttcatct
    10021 atagtgccgt aatcacccct tagaaaccct gcaatgagat ccccgaatac cggagcatca
    10081 aacctgggga aatggaggta ttcgactttg tgcccgcggg attccatata attggaaacc
    10141 atctttagtt gcgtggattt accggctcca tcaagtcctt caagtactat cagcataaat
    10201 gtctatcttt gtcttttgta ccacaaagtt aattgatatc ttcggattat ggaaaaacag
    10261 agagtagaaa ttatgggaat cgtcaatgta aatgacgact cattttttgc gcaaagccgt
    10321 gcaaaagaga aagatgcctt tattgcaagg gttgagatga tgttgaacca gggtgcagat
    10381 agcatagata tcggagctgt ttctacccgt cccaatatgg catccgtctc tttggaagag
    10441 gagtgggagc ggttgtcgcc ggtagtacaa ctttttgctt cccgcttcaa gggtgtgagg
    10501 ttttctgtgg atacatacag gggagagatt gtgaaaaggg tatatgaggc agccggacct
    10561 tttgttgtaa atgatatttc ggcaggggag gaggatcctc agatgctgga tagagttgca
    10621 gaatttgacc tggaatatat agcaatgcac cgcaaagggg attgtgtgac catgcaccat
    10681 aaatatgagt atgaggatgt ggtaggtgag gttgtggagt atttcaggaa gtttgacgag
    10741 c
//
LOCUS       DAOSIE010000018        10682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_6076_length_10682_cov_4.898278, whole genome shotgun sequence.
ACCESSION   DAOSIE010000018 DAOSIE010000000
VERSION     DAOSIE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10682
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6076_length_10682_cov_4.898278"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLI69_01250"
     CDS             complement(<1..259)
                     /locus_tag="PLI69_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
                     /protein_id="HOG41152.1"
                     /translation="MHKGKFVFSQLLDFIDKDVFLRISNKYGGNRYVKQFTCWNQLAV
                     LMFGQLSNRESLRDLVLATQAHASKAYHLGFGKHAVRSTLAD"
     gene            378..1502
                     /locus_tag="PLI69_01255"
     CDS             378..1502
                     /locus_tag="PLI69_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41153.1"
                     /translation="MKIHNKYQELMGKSSGELFAQFFIRIYQEIPQCVLAEFSSLKIL
                     QAPNFSDFRNIFRAKLEKIFLVPANTFDNVKGDFPIGFIIWDTSKDEVFEKTSADVYD
                     AKGNFVGTKVITAYDNLKYISDWLDINSTNISTNYIGHLASVGNDFQNQRMIFIDNEK
                     KENWKKGGRHTRITAENLIIASIFFAVRKVIPATWLNDRDQFLHPNNKWEEDTEFQND
                     CLAYTLFNNNISSSFGVNDWIPFTEQEVDAREKFASNFMINFINGKLKTDHIGGLFST
                     QTERTTPLIFSLEAAAVFDAGRELWKYYHKQPNCNVNASYYDIREHFQGRNEAGKMNN
                     TSSDETYTSLISNLRDKLRQLTYKIEPKVYQYGFLKKQHI"
     gene            complement(1700..1784)
                     /locus_tag="PLI69_01260"
     tRNA            complement(1700..1784)
                     /locus_tag="PLI69_01260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1748..1750),aa:Ser,seq:tga)
     gene            complement(1843..2490)
                     /locus_tag="PLI69_01265"
     CDS             complement(1843..2490)
                     /locus_tag="PLI69_01265"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOG41154.1"
                     /translation="MKISRGFKISLFVILLLIIDQIIKILVKTNMTLGQSIPVFGSWF
                     QILFIENNGMAFGMQFGGNVGKFILSIFRVVLVIAIIIYIRKLLKREETPTGVLIGLG
                     AILCGALGNIIDSLFYGMIFSESTPIQVAELFPPGGGYSGFLFGKVVDMFYFPIIDTT
                     LPEWVPVWGGRNFIFFRPIFNFADACITCGAIYLLIFKWKFFSKQPKTEESDSAK"
     gene            complement(2496..2876)
                     /locus_tag="PLI69_01270"
     CDS             complement(2496..2876)
                     /locus_tag="PLI69_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOG41155.1"
                     /translation="MNEKVRYSDEELQEFKELILEKLAQAREEYEMLRGAVNHSTSND
                     TEDTSPTFKVLEEGASSLSKEESGQLAARQYKFIQNLENALIRIENKTYGICRVTGKL
                     IPKERLRLVPHATLSVEGKNEMNK"
     gene            complement(2932..6297)
                     /gene="ileS"
                     /locus_tag="PLI69_01275"
     CDS             complement(2932..6297)
                     /gene="ileS"
                     /locus_tag="PLI69_01275"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOG41156.1"
                     /translation="MSFNEYKKLDLVETNRTVLEKWNKQDTFRKVLAAGENAPNFIFF
                     EGPPSANGMPGIHHVMARAIKDVICRYKTQSGYRVERKAGWDTHGLPVELSVEKMLGI
                     TKEDIGKKISVEEYNEACRKEVMKYTREWVSLTELIGYWVDMDNPYITYDNRYIETLW
                     YLLKIIYEKGLLYKGYSIQPYSPAAGTGLSSHELNQPGCYRDVKDTTAVVQFEIIKDT
                     KSQFLFEDSPLPLFFLAWTTTPWTLPSNTALSVGPNIEYLKVESLNPYTKEPAIYVVA
                     KALLNAHFNEKIPFRVIGEPIKGKELAGIRYEQLFPWVKCSEGAHKVVLGDFVTTEDG
                     TGIVHIAPTFGADDDKVAKANGVPPLIMVDAAGLNQAMVDPTGKFYALEDLDPVFVAE
                     RVNVEEYGKYAGRYVKNEYDPTLGPDDVTLDIDLSVDLKQQGKAFKIEKQTHNYPHCW
                     RTDKPVLYYPLDSWFIKTTAVQERMMELNDTIDWKPASTGTGRFGKWLENLQDWNLSR
                     SRYWGTPLPIWRTEDGTVEKCIGSVAELYEELEKSVSAGIMPSNPLKEKGFVPGDYSD
                     ENFAKIDIHRPYVDDLFLVSDDGRKMVRESDLIDVWFDSGAMPYAQETLAKVNSPDFG
                     QTADFIAEGVDQTRGWFFTLHAIATMVSDKCAFKRVISNGLVLDKEGNKMSKRLGNAV
                     DPFSTLDEYGADPLRWYMLTNAQPWDNLKFDMGGVDEVRRKFFGTLYNTYSFFALYAN
                     VDGFNGSEPEVPLSERQEIDRWLISLLNTLVKKSVEAYEDYDFTTAGRLIQDFVCDNL
                     SNWYVRLNRKRFWGGGLTPDKVAAYQTLHEALKTVALLSAPIAPFYMDLLWCDLVPGA
                     ESVHFERMPAYNEEYIDSALEERMDLAQKSSSMVLALRRKVNIKVRQPLAKLIIPVLD
                     RAIEEQLERVKEIILNEVNVKEMEFIHDTTGIITKKIKPNFKTLGKRYGKQMKEIASA
                     FATLSQQEISEIEKAGDYNLSLPSGEVTLSTEDYEITSEDMPGWLVATEGKLTVALDV
                     TVTDALRREGVARELINRIQNLRKESGFEVTDRINVFIQHNPEIEESLSDFKTYVCEQ
                     TLSKSITLTEECEDGTPVEWDEGELRISVVKLK"
     gene            6369..6854
                     /locus_tag="PLI69_01280"
     CDS             6369..6854
                     /locus_tag="PLI69_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOG41157.1"
                     /translation="MGVIDIILLVCFLPAFYFGIKNGLVKQIISVAVVYFGIVLSLRF
                     SDKVSTWILQLVTLSDFWVKAISFVLIFIVVAILLNLIGKLIEKILKIALLGWLNRLL
                     GVVVTLAIFVLLLSTIIHFVDAANSTFHFISEDKIAESKLYPSLLDLSYKLFPYFESF
                     F"
     gene            complement(6923..7141)
                     /locus_tag="PLI69_01285"
     CDS             complement(6923..7141)
                     /locus_tag="PLI69_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41158.1"
                     /translation="MKAVAAKTESKRQSKETAAYRSTKPLPVSGTIPDTVRSSSDAGN
                     ISLSLFIIRGSAKKSIGKVVDKKEKKYA"
     gene            7243..9321
                     /locus_tag="PLI69_01290"
     CDS             7243..9321
                     /locus_tag="PLI69_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOG41159.1"
                     /translation="MRRITNIRQHDRTDCAAACIASIAGWYGYSIPLTVIREASGTGA
                     YGTTVKGILDACREIGFTAKGYKSETGDTTPLLGLGCPAILHILKENGDLHFYVLYKI
                     NGKKAIVMDPAEGRRIKKDIDTLHKEWSGYLVIMRPDPDFTQFRGKRGHFSKYASLLL
                     LTKRDYSLSLAGAAAYIVAGICTALFLQHIIDNVLPAGDMRALGVTGALMILIMAAAL
                     FVGYGQVIFTLRTGITMDSYIILKYINHLFSLPVGFFKGRGAGELNSRIGDSMRIRSF
                     LIEGVTTILTSVLTLAVSFVLMFSFYWKLALMTFAFIPLFSTVYIIAGRINRKTSREI
                     IESAAEFEEKSVEGISSIKVIKYFGNGEHYYRNIERKYVTFAEKLYRGGRYSGIFATS
                     ADAISRLLTVCLILIGSLFIFKGKLSVGELVSFYSLTSFFSGPLSQLVKLTDDLAEFN
                     TASERIFEILDLKSEGGESLPYVPDRCEDILFHNVEFSYPGHEPLLSNFNITIPKGQI
                     TVIKGASGCGKSSLASLLMRDYKVQKGKITLGDIDISQIEIGQWRDYISIVPQDADLI
                     SGTILENITCHDPKPDVERVINILEELGMRQFISGLQMGLLTRTGEHGSRISGGQRQR
                     IALARVLYRNPFIYILDEATSSLDGESEAFILRKLQDLRDKGKTVILITHNKENALIA
                     DNTITMSAHS"
     gene            9377..9574
                     /locus_tag="PLI69_01295"
     CDS             9377..9574
                     /locus_tag="PLI69_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41160.1"
                     /translation="MEEKRLKELGLREMTSIELQSAGGTTLSAISLFEKVRAIINFIA
                     DYLPKLVKGIIDGWNSLKGPK"
     gene            9590..9781
                     /locus_tag="PLI69_01300"
     CDS             9590..9781
                     /locus_tag="PLI69_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41161.1"
                     /translation="MREDFTIPGLRPIGVSESACIIGGIDKKAQDALYYIGYVIGLVA
                     QYICSLFKKIKNVFGGTNA"
     gene            9848..>10682
                     /locus_tag="PLI69_01305"
     CDS             9848..>10682
                     /locus_tag="PLI69_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG41162.1"
                     /translation="MEKTRKRLALTAVMLIASIFTSSGQNWTLEQCINHALEHNHDIR
                     SARASLEEIESAFNELSWSLIPRIGGEMGQDFNWERLSPYKLKGKQSTNVSIGLNVTL
                     FDGLRGYFSIKEAGHSLNQAKNSLEGVRDAVKVSVIRSCLEMVLARELHSIAEYNFES
                     IVRQMEKCRKMVLAGLRPLSSLMELEAQVAQEQYTITATAAEIESSRIALMQLLNLPY
                     ESRFNVEVPPIDTLPPEDLPELAAYKLADAHPSIRAAKAALEADKAAIGVAMSSIYPT
                     IA"
BASE COUNT         2951 a         2421 c         2375 g         2935 t
ORIGIN      
        1 catcagcaag agtactcctg acggcgtgct taccgaaccc aagatggtat gcctttgagg
       61 catgagcctg ggttgccagc acaaggtctc tgagactttc acggttggaa agctgaccaa
      121 acatcagtac cgcaagttga ttccagcagg taaattgctt gacatagcga tttccgccat
      181 atttatttga tattctgagg aatacatcct tatcaataaa atctaaaagc tgtgagaaca
      241 cgaattttcc tttatgcata tgccattcta cgttagtctg gcaaagataa tttcaaatcg
      301 gcgcggtctc aaaacctctg taattaccta attttcaata ttttcaaaga acttttatga
      361 ttttttaccg gacactaatg aaaatacata ataaatatca agaacttatg ggcaaatcta
      421 gtggcgaatt atttgctcaa ttttttataa gaatttatca agaaatccct caatgcgtgc
      481 ttgcagaatt ttcatctttg aaaattctgc aagcaccaaa cttttcggat tttcgcaaca
      541 tttttcgagc aaagctcgaa aaaatttttt tggttcctgc aaatactttt gataatgtca
      601 aaggagattt tcctattggt tttattattt gggacacatc aaaagatgaa gtttttgaaa
      661 aaactagtgc agacgtgtac gatgcaaaag gtaattttgt cggaacaaaa gtaattacag
      721 cttatgacaa tttaaaatac ataagcgatt ggcttgatat taattccaca aacatttcta
      781 ccaactacat tggtcatttg gcatctgtcg gtaatgattt tcaaaatcaa cgaatgattt
      841 ttattgataa tgagaaaaaa gaaaattgga aaaaaggcgg acgtcatacc agaattacag
      901 cagaaaattt aattattgct tccatatttt ttgcagttcg aaaagttatt cctgccactt
      961 ggctgaatga ccgcgaccaa tttctacacc caaataacaa atgggaagaa gataccgagt
     1021 ttcaaaatga ttgtttggca tacacacttt ttaataacaa tatctcaagc agttttggtg
     1081 taaacgattg gattccattt acagaacaag aagtagatgc gagggaaaag tttgcaagta
     1141 attttatgat caactttata aacggcaaac ttaaaaccga ccatattgga ggtttattta
     1201 gcacacaaac agaaaggaca acgccactca tcttttcact agaggcagca gctgtttttg
     1261 acgcaggtcg tgaactttgg aaatattacc acaaacaacc taactgtaat gttaatgctt
     1321 catattacga cattagagaa cattttcaag gcagaaacga agcaggtaaa atgaacaata
     1381 ccagttcaga cgaaacatat actagcttga ttagcaacct aagagataaa ctcagacaac
     1441 ttacctacaa aatagagcca aaagtttatc agtatgggtt cttaaaaaaa caacatattt
     1501 gatagagagg gactctctct ccgggatttg gtcgcatacg ctcccacatc cctatccact
     1561 cgccgtgggg cccttacaag tcgaaaggcc tgaagcggag cttcagtttt tatacaacaa
     1621 atcccctttt gagcaagctc aaggggattt atttgtataa aaaaaccgaa tgactttcgt
     1681 cattcggcct ttcgacttgc ggagagagag ggattcgaac ccccggaagc tttcacttca
     1741 acagttttca agactgccgg tttaaaccac tcacccatct ctccttccct ttggtttggg
     1801 agtgcaaaga tatattaaaa aaacatttac tcaaaaaaaa ggttattttg cggagtcgga
     1861 ttcttcggtc ttaggctgct tggagaagaa tttccatttg aaaatcagga ggtagatggc
     1921 tccgcaggtt atgcacgcat cggcgaagtt gaagatggga cggaagaaga taaagttgcg
     1981 tccaccccaa acgggtaccc attcggggag tgtggtgtct atgattggga aatagaacat
     2041 gtctaccact ttgccgaaaa ggaatcctga atagccgcct cctgggggaa atagctcggc
     2101 tacctgaatg ggtgtgcttt cgctgaatat catcccgtag aaaagagagt caatgatgtt
     2161 gccgagagca ccgcacagga tggctccgag tccgatgaga acacctgttg gagtctcttc
     2221 gcgcttgagg agttttctga tatatatgat gatggctatg acaagcacca ccctgaagat
     2281 agagaggatg aatttgccaa cgttgcctcc gaactgcatt ccgaaggcca tgccgttatt
     2341 ttcaataaag agtatctgga accaactgcc gaagacaggg atgctctgac ctaaagtcat
     2401 gttggtctta accaggattt tgataatctg gtcaataatc aaaagaagga ttacaaagag
     2461 ggatatctta aaacctctgg aaatcttcat ctttgttatt tgttcatttc attcttaccc
     2521 tcaacgctga gtgtagcgtg aggcacaagt ctaagtctct ccttaggtat cagtttaccg
     2581 gttacacggc agataccgta ggtcttgttt tcgatgcgaa taagggcatt ctcgaggttc
     2641 tgtatgaact tgtactgacg tgcagccagt tggcccgact cttccttgga aagggaagaa
     2701 gcgccctcct ccagcacctt gaaggtggga gatgtgtcct ctgtatcgtt acttgtgcta
     2761 tggtttacag cacctctgag catttcatac tcttctctgg cttgagccag cttttcaagg
     2821 atgagttctt tgaactcctg aagctcttca tcagagtaac gtaccttttc atttatttct
     2881 actggtttgc ccatgacttt atatgttggt taaagtgtaa aaatagcaat attatttcaa
     2941 tttaacaaca gaaatcctca attctccttc atcccactct accggagtac catcttcaca
     3001 ttcttcggtg agtgtaatgg attttgagag tgtctgctcg catacatagg ttttaaaatc
     3061 acttagtgac tcctcaattt caggattgtg ctggatgaat acgtttatac gatcagtcac
     3121 ctcaaatccg ctctctttgc gcagattctg aatacgattg atcagttcac gtgccactcc
     3181 ttcccttctg agggcatcgg tcacagtgac atccagcgct acggtaagtt tgccttcggt
     3241 agcgacgagc cagccgggca tatcctcgga tgttatctca taatcctcag tagatagcgt
     3301 cacttctccc gatgggaggg aaagattata gtctccggcc ttctcgattt ccgaaatttc
     3361 ctgctgcgaa agtgttgcaa aggcggatgc tatctccttc atctgtttgc cgtatctctt
     3421 gccgagagtc ttgaagttgg gttttatctt ttttgtaata atgccggtag tatcgtggat
     3481 aaattccatt tccttaacat tcacctcatt gagaatgatc tccttaaccc tctcaagctg
     3541 ctcctctata gcacggtcga gcacgggaat gatgagttta gccagcggct gacgcacctt
     3601 tatgttgacc tttctgcgaa gcgccaagac cattgaagag ctcttttgag ccaaatccat
     3661 gcgctcttca agagcggagt ctatatactc ttcgttgtag gcaggcatac gctcaaagtg
     3721 caccgattca gcccccggta cgagatcgca ccagagcaga tccatataaa atggagctat
     3781 cggagctgag agcagggcca ccgtcttgag tgcctcatgg agggtctgat aagctgcaac
     3841 cttatcagga gttagcccac cgccccagaa gcgcttgcga ttgaggcgta cataccagtt
     3901 gctgaggttg tcgcagacga aatcctgaat cagtctgcct gcggtggtaa aatcatagtc
     3961 ttcgtatgct tcaaccgact ttttgacgag agtgttaagc agcgaaatta gccatctgtc
     4021 gatctcctgg cgctctgaca ggggcacttc cggttcagaa ccgttgaaac cgtccacatt
     4081 tgcataaagt gcaaagaatg aataggtgtt gtagagggtt ccgaagaatt ttctacgtac
     4141 ctcatccact ccacccatgt caaacttcag gttgtcccag ggttgcgcat tggtaagcat
     4201 ataccatctg agcgggtcgg caccatattc atcgagggtc gagaatggat ccactgcatt
     4261 gccgaggcgc ttgctcatct tgttgccctc tttgtccaga acaagtccgt tggagataac
     4321 tctcttaaat gcgcacttat cgctcaccat ggtagctatt gcatggaggg taaaaaacca
     4381 acctcttgtc tggtcaacgc cttcggcgat gaaatccgca gtctgaccga aatcggggct
     4441 attgaccttt gcaagggtct cctgagcata gggcatcgct cccgaatcga accagacatc
     4501 aatcagatcg ctctccctta ccatcttgcg gccatcatcc gaaacgagga aaaggtcatc
     4561 gacgtaggga cggtgtatat ctatcttcgc aaaattttca tccgaatagt cgccggggac
     4621 aaatcccttc tccttcaaag ggttggaggg catgatccct gcggataccg atttctccag
     4681 ttcctcgtaa agctctgcta ctgaaccaat acatttctcc acagtgccat cctcggtacg
     4741 ccaaatagga agcggagtac cccaatagcg acttctggag agattccagt cctggaggtt
     4801 ctccagccat ttgccgaaac ggccggtacc tgtggaagca ggcttccagt caatggtatc
     4861 attgagttcc atcattctct cctgcacggc ggtagttttg atgaaccatg agtcaagcgg
     4921 gtaatatagc accggcttat cggttctcca gcagtggggg tagttgtgtg tttgtttctc
     4981 tattttaaag gcctttccct gctgtttcag atctacggaa agatcgatgt ccaaagtgac
     5041 gtcatcaggg ccgagagtgg ggtcatattc attctttaca taccttccgg catactttcc
     5101 atactcctca acattcactc tctcggcaac aaaaacggga tcaagatcct caagagcata
     5161 aaattttccg gtagggtcga ccatagcctg attgaggcct gcggcatcca ccattatcag
     5221 cgggggtacg ccgttggcct ttgctacctt gtcgtcatcc gcaccaaagg ttggggcaat
     5281 atgcacaata ccggtaccat cttcagtggt cacaaagtca cctaatacta ccttatgagc
     5341 gccttcagag cactttaccc aagggaacag ctgctcgtat cggatacctg ccaattcttt
     5401 tcctttgatc ggctccccta ttacgcggaa ggggatcttt tcattgaagt gagcatttag
     5461 gagggccttg gccaccacat agatggcagg ctctttcgta tagggattga gactctcgac
     5521 tttaagatat tcgatatttg gcccgacact aagtgcggta ttggagggta gcgtccaggg
     5581 agttgtggtc caggcaagga agaatagagg cagcggacta tcctcgaaaa ggaattgcga
     5641 ctttgtatcc ttgattatct caaactgcac gaccgcagtt gtatccttaa cgtcacgata
     5701 acatcccggc tggtttagtt catgcgaact gagtcccgtg ccggctgcgg gggaataggg
     5761 ctgtatggag tagcctttgt agagcagacc cttttcatat attattttga gaaggtacca
     5821 caaggtctct atatagcggt tgtcataggt aatgtaggga ttgtccatat ccacccagta
     5881 acctataagt tcggtaagtg agacccattc cctggtatat ttcatcacct ccttacggca
     5941 ggcttcatta tattcctcta cggaaatctt tttcccaata tcttccttcg tgataccgag
     6001 cattttttcc acactaagct ctaccgggag accgtgagta tcccatcccg ccttgcgctc
     6061 cactctataa ccgctctgtg tcttatacct gcaaataaca tcttttatgg ccctggccat
     6121 cacatggtgg ataccgggca tgccgttagc cgaaggggga ccctcaaaaa agatgaaatt
     6181 cggcgcattc tctcctgcag caagtacttt gcggaaagta tcctgcttgt tccatttctc
     6241 taatactgta cgatttgtct ctaccaaatc gagctttttg tactcgttaa aactcatatt
     6301 tcattcattt gaatttgcaa agatacgata attcactaat tttgaccaat cattaaaaaa
     6361 ttaagtatat gggagttata gacattatcc ttctagtctg ctttctaccc gcgttttact
     6421 ttggcatcaa gaatggtctt gtcaaacaaa taatatccgt ggcagtcgta tatttcggca
     6481 tcgtactctc tctgagattt tcagataagg tttctacctg gattctgcaa cttgtcacac
     6541 tatccgattt ctgggtaaag gctatttcat tcgttttgat atttatagtt gtagctattt
     6601 tgctcaatct catcggtaaa cttatagaaa aaatattaaa aatagccctg ctcggatggc
     6661 taaacagact cctcggagtg gttgttactc tggcaatttt tgttctgctg ctttccacca
     6721 ttatccattt tgtagatgcc gccaacagca cattccattt tatttccgaa gataaaatag
     6781 ctgagtcaaa gctatatccc tctttgctcg acctctcata caagcttttt ccctattttg
     6841 agtcgttttt ttaaaaaatt ctgatttttt caaaaaaatt aaatggctga cgcttgaagc
     6901 aacaactgtt atagttgctt tctcaagcat attttttctc ttttttatct actactttgc
     6961 ctatggattt tttggcagag ccacggatta tgaaaagaga aagagaaata ttgcctgcat
     7021 ctgaactcga ccgaacagta tcgggaatgg tgcctgacac cggaaggggt ttcgtgcttc
     7081 tatacgcggc agtctccttg ctttgtctct tactctccgt tttcgcggct acagctttca
     7141 tctaagaggg agcctgtgtt gaagatatgt cctgatggcg gacgggacac tgaagggtca
     7201 ggatgactcc ctctttttaa tgaatcgaag aggagaatgg gaatgagaag gataacaaat
     7261 ataaggcaac atgacaggac ggattgcgca gcagcctgca tcgcctccat agccggttgg
     7321 tatggataca gcataccatt gaccgtcatc agagaggcgt ccggtacagg tgcatacggc
     7381 accacggtca aaggtattct ggatgcttgc cgggagatag ggttcacggc caaaggctat
     7441 aagtcggaaa ccggcgatac cactcccctg cttggactgg gctgccctgc cattcttcac
     7501 attctcaagg aaaacggaga ccttcatttc tatgttctct acaaaataaa tggtaaaaaa
     7561 gccatcgtta tggatcctgc agagggccgt cgtattaaaa aagatatcga cacgttgcat
     7621 aaagagtgga gcgggtacct ggtcataatg agaccggacc ccgatttcac ccaattccgc
     7681 ggaaaacgcg gacatttctc taaatacgct tcactactct tactaacaaa aagggattac
     7741 tccctttctc ttgccggggc tgctgcctac attgtggccg gcatctgcac cgctcttttc
     7801 ttgcaacata tcatagataa tgtactcccg gccggagata tgagagctct gggtgtcaca
     7861 ggcgcactta tgatcctcat aatggctgct gctctatttg tcgggtacgg tcaggtgatc
     7921 ttcaccttgc gtaccggaat tacgatggat agttatataa ttctcaaata tataaaccac
     7981 cttttctctc tgcctgtcgg atttttcaaa ggcagaggcg cgggcgaact caactccaga
     8041 ataggggatt ctatgcgcat ccgctccttt ctgatagaag gtgtcaccac tatactaaca
     8101 agcgttctca cacttgcagt ctcattcgtg ctgatgtttt cattttactg gaagctcgca
     8161 ttgatgacat ttgctttcat cccgcttttc tctacggtct acataattgc cggaaggatt
     8221 aacagaaaga ccagcaggga gataatagag agtgctgccg aatttgagga gaagagcgta
     8281 gaaggcattt cctccataaa agtaatcaag tatttcggta acggagaaca ctattatcgc
     8341 aatatcgagc gaaaatacgt cacatttgca gagaagcttt atcgcggagg gcgttattcg
     8401 ggcatatttg caacatctgc ggatgctatc tccagattgc tgacagtttg cctgatactc
     8461 ataggttcgc tgtttatctt taaaggaaaa cttagcgtcg gggaactggt ttccttctac
     8521 tctctcacct cattcttttc cgggccatta agccaactgg tgaaattaac ggacgattta
     8581 gccgagttca acacggcatc agagaggatt tttgaaatcc tcgacctaaa aagcgaaggt
     8641 ggagagtccc ttccatatgt gccggacaga tgcgaggata tattattcca caacgtggaa
     8701 ttttcctatc ccggacatga acctttgctg agcaacttca acataactat accaaaagga
     8761 caaataacgg ttataaaagg agccagcgga tgtgggaaaa gttctctcgc ctcactgctt
     8821 atgcgggact ataaggtaca aaaaggtaaa ataacccttg gcgatatcga catcagtcaa
     8881 attgagatag gtcaatggcg tgactatatt tcgattgtac cccaggatgc tgaccttata
     8941 agcggtacaa tactggaaaa tatcacctgc cacgacccta aacctgatgt ggaacgggta
     9001 ataaacatcc tcgaagagct gggtatgcgt caattcattt cgggcttgca gatgggactc
     9061 ctgacacgca ccggggaaca cggatcccgc atttccggtg gccagagaca gcgtatagca
     9121 cttgccagag ttctctaccg taatccattc atatacatac tggatgaggc cacctcatcc
     9181 ctggatggcg aatcggaggc atttattctg cgcaaactgc aagatctaag ggataagggc
     9241 aaaaccgtaa tattgattac acacaataaa gagaatgctt tgattgcaga caatactatc
     9301 acgatgagtg cgcactcata gaggtcattt ccgggcacct gaccaaagaa cggagtaaca
     9361 caaaaaattt agctttatgg aagaaaaaag attgaaagaa ctgggattga gggaaatgac
     9421 ttccattgaa ctccagtcgg caggcgggac aactttgagt gccatctcgc tgtttgaaaa
     9481 agtaagggcc atcatcaatt ttattgctga ctatctgcca aaacttgtaa aaggaattat
     9541 tgacggatgg aacagtctca aaggaccaaa atgatggagg aagtgaacta tgagagagga
     9601 tttcacaata ccgggactca gacctattgg ggtaagcgag tcggcctgca tcatcggcgg
     9661 catagataaa aaggcccagg atgcacttta ctacatcggt tatgtaattg gactcgtagc
     9721 gcagtacatc tgctctctgt tcaaaaaaat aaaaaatgtt ttcggaggaa cgaatgcata
     9781 accattaata ggcgggaatt ccgggtgctg acgacacccg ggactccctt ttatcacaaa
     9841 tcaaaccatg gaaaaaacca gaaagcgcct cgcgctaacg gcagtaatgc tgattgcaag
     9901 catattcact tcttcggggc agaattggac tttagagcaa tgtataaacc atgcattgga
     9961 gcacaaccac gatatcagaa gtgcccgggc ctcactggaa gagattgaaa gcgcattcaa
    10021 cgagctctca tggagtctaa ttccccggat cggtggtgaa atgggacagg atttcaattg
    10081 ggaacgactc tccccctaca agctcaaggg taagcaaagt accaacgtct ccattggact
    10141 taatgtgacg ctgttcgacg gactaagggg atacttcagc atcaaagagg ccggtcactc
    10201 gctcaatcag gcgaaaaact cccttgaagg ggtgcgtgac gcagtcaaag tcagcgtaat
    10261 tcgcagctgc ctggaaatgg tgctggcgag agaattacac tccatagcgg aatacaattt
    10321 tgaaagcatc gtcagacaga tggagaagtg ccgtaaaatg gtgctggccg ggctgcgccc
    10381 attgagcagc ctaatggagc ttgaggcaca ggtagctcag gaacagtaca ctattaccgc
    10441 aactgctgct gagatagaga gcagccgaat tgctctgatg cagctgctta acctccctta
    10501 tgagagcagg ttcaacgtag aagtgccacc gattgatacg ctgccaccgg aggatttacc
    10561 ggagttggct gcttacaagc tggctgatgc gcatccttcc ataagggcag caaaggctgc
    10621 actggaagcg gataaggctg ccataggagt agctatgagc agcatttatc ccaccatagc
    10681 ac
//
LOCUS       DAOSIE010000019        10605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_6129_length_10605_cov_4.144455, whole genome shotgun sequence.
ACCESSION   DAOSIE010000019 DAOSIE010000000
VERSION     DAOSIE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6129_length_10605_cov_4.144455"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2648
                     /locus_tag="PLI69_01310"
     CDS             <1..2648
                     /locus_tag="PLI69_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOG41163.1"
                     /translation="IMKRRLFFTFLTAFAICFIQAAGRSSTNSILIKGGLVYDGSLNK
                     PVKCDVLIIGDRIAFVGDYTGKTADRVIDASGYIVTPGFIDPHSHSDGDFLKERKSNE
                     NYLQMGVTTVLCGQCGSGSWPMTETFEKMRNQGMGTNCGLFLGHSPLRKAVMGDDQMR
                     DATKEEIEKMKQLAIEAMNLGAFGISTGLYYLPGTFTSTEELIEVISAISDYGGIYAT
                     HIRSEGTSGVGLDSAINEAIRIAREAQVQLNISHVKSSGGTKGSSGRIIAAIEAAQNE
                     GLYITADQYPYLASSTSLSSALLPKWAQFGGRNAYLRRFDHPDTLRMIKEEISENISN
                     RGGAGSIMIIKGPIKEYIGKYLNYIAKEMKLSPVDAVVEILRQGAPSIANFNLSEKDV
                     CNYMKQPWVMTCTDGSFGGHPRATGSYPHKIQHYVLKKNILTMEQMIHKSTGKVAQTY
                     GIEGRGFIKEGYYADILVFKPKEFRANSTYADASAYASGLRLQLVNGEITIENSKYNG
                     TLAGKPLKMKNSAKDTAIFESLMGQRMKKFGAIGLITAVVRNNEIVYHHSFGSKDLEG
                     HSFRGDERDVVRIASISKSFCALSLMQLMEQKKIDLDADVGEILGFKVRNPKYPDDKI
                     TISMLMSHTSSLNDSQGYKTLDKINPLKNPNYNKCYSNYAPGMGYRYCNLNFNMLGAV
                     VEKISGVRYDRYVKQNILDPLGLTGSLNMADIPDTTICVPLYEYNEGKGGFIHRPEAY
                     DPLAKSDQDYVLGYDPALFSTTGGMKMSALDLARYMMMHINYGTLDGVSIINESSARL
                     MQSIQTPTDSPGIYYGYAMRTMQDVRIPGIVTHGHTGSAYGMKSSMTFDPIRKNGVIT
                     LCNGHKSKERPTESFDRLLFAFFFR"
     gene            complement(2652..4316)
                     /locus_tag="PLI69_01315"
     CDS             complement(2652..4316)
                     /locus_tag="PLI69_01315"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG41164.1"
                     /translation="MKRIHPIIFMIAAVLTFWSCSKEFLDTAPTSSVDEASIFSSTEN
                     AMMAINGIHRMMYEGNSSGTTTSWNGQGGYQTFCLHLAFMTDDVVWTYDNVMYKFCAQ
                     LTHHRDLSHKYNDLNYYWKLFYRVINNANKVIEAYDAGMPGSEQYGYMAVGEAYAYRG
                     FALFELVQAWAKRYNPAGNNTQPGVILRLTPSPDPMARSSVEETYAQILADLGTAFEY
                     LDKCTIKKVNKSHFDKYVVRGIRARVYLTMGKWSEAAADAEYVIKNSGAKLQDDTYTT
                     VVNRASDATNTEWLWGLIAKEDNSQGGGNRSWHNFISNNYAAYNRNSPRAILNLLYKT
                     IPESDVRKRLWVEDPYTAKELYLPASSSARRACWMSQKWIVDDNQTQNDFHDVAYMRL
                     PEMYLIAAEGYARAGQNDKAQSMLYTIANHRDPKYAKPITSGEALVEAVIWQRRVELW
                     AEHGTRWHDLKRLDQAIDRGPKPRDGYNQGGTANGWGTSAKVAPWNNPKVPPLDPLAS
                     NYNMYGEQVTGEQARKTGKPSVDEVWQWLIPYNEIASNPLCEQNPL"
     gene            complement(4329..7544)
                     /locus_tag="PLI69_01320"
     CDS             complement(4329..7544)
                     /locus_tag="PLI69_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG41165.1"
                     /translation="MNYKHLLITVMVLLLFLPLRMLGQGGNISVKGTIFDENHDPVPF
                     ASVVLKGTTSGVAADADGKYSISVPRNSVLIFSSIGYLNQEIPVNGRAEIDVFLIPDS
                     EALEEVMVVAYGTATKTSFTGSAGKISGEKIQLIPNTNPLNTLNGTTPGIRLTSTLGQ
                     PGADATITIRGIGSINGNIDPLIVMDGIIYSGVLSSIPSGDIESITVLKDAASTALYG
                     ARAANGVIMVTTKQGKSEKATINVKVSTGWVTREFKDYETMGIKDYMETYWRLYYNNH
                     ILNGVTPEDAARRASNSVATSLNFTEEYNPWSCGITEVVDINGKYNSSATFKWLDDTD
                     WRNGIEQVGLVQDYSVSANGRHGKNSYYASVGYSDNEGYIVGSGFERYTARANVSTKA
                     SDWLNIGVNMASTLSTQYGIQSTSQGTMGNVFMIARRMPPVYGVHLHDNTGAYILDEH
                     GNPIYDFGEGYTTASGLTIPGRVIYSGVNAPRQLKDRFNQQERTMMDVKPYIEISFLK
                     DFKFTANAALYNSNYVSHSATPFYSELTSNTPATSKTISNTRTWTFNQLLTWGHNFGK
                     HHVDALLGHESNSYTYSLNSSVSMRYQIVIGDNYEFDNYIEVSAQPSSYKNVYNTEGY
                     FARANYDYDGKYFFSSSFRRDGSSRFARNSRWGNFWSVGGSWVLDRENFMRGIDWING
                     LKLRASVGTVGSDDLGSYFPWMALYVINQNVDEPGYTQSMTSTGNPDLQWEVSTNWDV
                     AVEFSLFNRRLRGSLEYFDRRTTNLLMEVTLPPSTGLTSHNENAGGLYNRGIEFQLDY
                     DIFKTGDINWNVGLTGTYLKNRITSLPIEPFLRSASYNKVEQGHSVYEWWLYQWAGVD
                     PQTGLNIFEIADEFISDTSEDIVSVNGINYTTNMAKAREDYSGSSIPDLFGGINTSLS
                     WKGFTLSLDLYYQLGGWTYDRAYENVMIGALVSTNSVNLSPDLAANMWRKPGDVTNVP
                     MLTSNATYASNIHAARSTRWLTTTNMLEINSLNLAYSLPKKWCERLKLSKIQVYLSGD
                     HLYVFNARRGLNSNYSLSNYDSGGDRFSPSRTISLGVNITL"
     gene            complement(7912..8394)
                     /locus_tag="PLI69_01325"
     CDS             complement(7912..8394)
                     /locus_tag="PLI69_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41166.1"
                     /translation="MKTKSAIITMLALFFLIGGTGCDRETPNENLQIKDFSYQGCKEI
                     VTNLTKSADKEEYIEYRAIGDGYLRIKHVNAVFNCCPGTIKSDLSVEGNTITIVETET
                     DPQCKCICDYDLEYTLGPLSSNTEYILIFCNNKGEYLRFTIKYKSSLEGKVIVKKFKG
                     "
     gene            complement(8405..8872)
                     /locus_tag="PLI69_01330"
     CDS             complement(8405..8872)
                     /locus_tag="PLI69_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41167.1"
                     /translation="MLSKFKPFLAVKMKMITILILGFLFTLFFVSGCEKRPQYEIYEN
                     HDITVCGVKDPLRNMEWIKNYCAEHSKAYFIKISLYQDLETNANYFVIESKSDFIPGR
                     SPSCIFSESVYSCDGVQLLFYGVEGPIPEGWDEFFEKVKLVGTIWSCERVYNK"
     gene            complement(8856..9311)
                     /locus_tag="PLI69_01335"
     CDS             complement(8856..9311)
                     /locus_tag="PLI69_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41168.1"
                     /translation="MKTKSILLSVIFLFFLIGGTGCEEKNLTTNDAIIIKELPKMYLL
                     DGKMKQGEGKVVQSRQELLALFSQDEIDKYPDLKGIDFSTQTLLIGCDGLPSLAHLNY
                     AFYKNKDDDFIFFVEIGGDATQPDPHFHYGIVVEKLAKTAKVIFNVVKI"
     gene            complement(9318..9788)
                     /locus_tag="PLI69_01340"
     CDS             complement(9318..9788)
                     /locus_tag="PLI69_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41169.1"
                     /translation="MKTKTVLITMLALCFLIGGAGCDKETSNESLRIKDFSYQGCKET
                     KSLLTSYHGEESIEYKAVANGYLHIKHINAVFNCCPDTIQADVSMKDNIITIVESETN
                     PICDCICNYDLEYTFGPLTSNKEYILIFSSGAYVFSKLIIKYSPSLEGRITVQH"
     gene            complement(10042..10114)
                     /locus_tag="PLI69_01345"
     tRNA            complement(10042..10114)
                     /locus_tag="PLI69_01345"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10079..10081),aa:Pro,seq:tgg)
     gene            10213..10299
                     /locus_tag="PLI69_01350"
     tRNA            10213..10299
                     /locus_tag="PLI69_01350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10247..10249,aa:Ser,seq:gct)
     gene            complement(10362..>10605)
                     /gene="rhuM"
                     /locus_tag="PLI69_01355"
     CDS             complement(10362..>10605)
                     /gene="rhuM"
                     /locus_tag="PLI69_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOG41170.1"
                     /translation="NRNIPMTMQDWATHLDRILMATEHELLTNPGTISAEVARKHAET
                     EWEKYRIVQDRLFESDFDRFIKLNSTAQRLKLIINS"
BASE COUNT         3042 a         2345 c         2187 g         3031 t
ORIGIN      
        1 tcattatgaa aagaagactg ttttttactt ttttgacggc cttcgccatt tgtttcatcc
       61 aggcagcggg gcgctcttcc accaactcca tactcataaa aggcggtctt gtatatgatg
      121 gctccctcaa taaacccgtc aaatgtgacg tactgatcat aggggaccgg attgcatttg
      181 tcggagacta taccggcaaa acggccgaca gagtaatcga tgcttcaggt tatattgtca
      241 ctccgggctt tatagaccct cattcacatt ccgatggaga tttcctcaaa gagagaaaat
      301 caaacgaaaa ttatttacaa atgggggtga ccacagttct ttgcggacag tgtggatcgg
      361 gctcctggcc tatgaccgaa acttttgaaa agatgcgtaa ccaaggcatg ggtactaact
      421 gcggactctt tcttggccac tctcctttgc gcaaggctgt tatgggagat gaccagatga
      481 gagatgccac caaggaagaa atcgaaaaga tgaaacaact cgcaatcgaa gcgatgaatc
      541 tgggcgcctt tggaatttct accggacttt actatcttcc gggaacattc acaagtactg
      601 aagaacttat tgaagttata tctgccatct ccgattacgg tggcatttac gccacccata
      661 tccgtagcga agggacctcc ggtgtggggc tggatagtgc aatcaacgaa gccatacgca
      721 tagcacgcga agctcaagta cagttaaata tctcacatgt caaatccagc ggggggacaa
      781 aaggcagttc aggcaggatc atagctgcca tagaagccgc tcagaacgag ggactgtata
      841 ttactgcaga ccaataccct tacctagcct caagcacctc actctcttct gcactacttc
      901 ctaaatgggc ccagttcggc ggcagaaatg cttacttgcg aagatttgac catcctgata
      961 ccctgcgtat gataaaagag gagatcagtg agaatatctc caatcgaggt ggcgcaggct
     1021 ccataatgat cataaaaggt cctatcaagg aatatattgg aaaatacctc aactacatag
     1081 caaaagagat gaaactttcc cctgtagacg cagtagtgga aatccttcgc cagggagctc
     1141 cctccatcgc taacttcaat ctaagtgaaa aagatgtatg caactatatg aaacagccct
     1201 gggtgatgac ctgtactgac ggtagttttg gagggcaccc gcgtgccacc ggctcatacc
     1261 cccacaaaat acaacattat gtcctcaaaa agaatatcct tacgatggag caaatgatcc
     1321 acaaaagcac cggaaaagtg gcgcagacat atggcattga ggggcgtggt ttcatcaaag
     1381 agggatatta tgcagatatt ctggtcttta aaccaaaaga gtttcgagcc aattccacct
     1441 atgccgacgc ctcagcatac gcatcaggac tgagacttca gctggtcaac ggagaaataa
     1501 caatcgaaaa cagcaaatac aatggtactc ttgccggcaa accgcttaaa atgaaaaaca
     1561 gtgccaagga tactgcaatc tttgagtcgc tgatggggca gagaatgaaa aaatttgggg
     1621 ctatcggact cattactgct gttgtcagaa acaacgaaat cgtatatcat cactctttcg
     1681 gcagtaaaga ccttgaaggc cattctttcc gaggagacga gcgtgatgtc gtgcgcattg
     1741 cctccatttc caaatctttc tgtgctcttt cactaatgca gttgatggaa cagaaaaaga
     1801 tagacctgga tgccgatgta ggggaaattc ttggtttcaa ggtaagaaac ccaaaatatc
     1861 ctgatgataa aatcactatt tcgatgctta tgagtcacac atcgtcacta aatgattccc
     1921 agggctacaa aactcttgac aagattaatc ctttgaaaaa ccccaactac aacaaatgtt
     1981 atagcaatta tgctcctgga atgggataca gatattgtaa tcttaatttc aatatgctcg
     2041 gtgcagtggt ggaaaaaatt tccggcgtta ggtatgatcg ctatgtcaag cagaacatat
     2101 tggatcctct cggtcttacc ggcagtctaa acatggccga cataccggat acgacaattt
     2161 gtgttccgct ttatgaatac aacgaaggta aagggggttt tatccataga cctgaggcct
     2221 atgatccttt ggcaaagagt gatcaagact atgtattggg ctatgatccc gctcttttct
     2281 ccaccacggg tggaatgaaa atgagtgcac ttgatctagc ccgatatatg atgatgcaca
     2341 tcaattacgg tacacttgat ggagtaagta ttattaatga gagcagtgcg cgcttgatgc
     2401 aatccataca gactccgacc gattccccgg gtatatatta tggctatgcg atgaggacga
     2461 tgcaggatgt ccggattccc ggcatcgtaa ctcacggtca caccggatcg gcatacggta
     2521 tgaaaagcag tatgactttt gatccgattc gcaaaaatgg agttataacg ctatgcaacg
     2581 gacataaaag caaagagcgg cctaccgaaa gtttcgatag actgctcttt gcattctttt
     2641 ttagatagag actacagcgg attctgctcg caaagcggat ttgatgcgat ctcgttgtat
     2701 ggaatcaacc actgccaaac ctcatcaacc gaaggttttc ctgtcttcct tgcctgttct
     2761 cctgtcacct gctctccata catgttgtag tttgaagcca gaggatcaag aggaggtact
     2821 tttggattgt tccaaggagc caccttggca cttgtgcccc atccgtttgc cgtaccaccc
     2881 tgattgtagc catcgcgtgg cttgggacca cggtctatcg cctggtcaag gcgtttaagg
     2941 tcgtgccagc gggtaccatg ttcagcccat aattcaaccc ttctctgcca tatcaccgct
     3001 tcaacaagag cctctccgct tgttatcggc ttcgcatatt tgggatcgcg atgattcgct
     3061 atagtgtata gcatactctg cgctttatca ttttgccctg cacgtgcata gccctctgcc
     3121 gctatcaaat acatctcagg aagccgcata taggcaacat catggaaatc attctgtgtc
     3181 tgattgtcat ccacaatcca tttctgcgac atccagcaag ctctacgcgc agatgaacta
     3241 gccggtagat atagttcctt ggcagtatag ggatcctcca cccacaggcg tttcctcaca
     3301 tctgactcag ggattgtttt atagagcaga ttgagaatag cccttgggct gtttctgttg
     3361 taggctgcat aattattgga gatgaaattg tgccaagagc gattaccacc accttgtgag
     3421 ttatcttctt ttgctatgag tccccataac cattcagtat tggttgcatc actggcgcgg
     3481 ttgaccacag tggtataggt atcatcctgg agcttagctc cggaattttt gataacgtac
     3541 tcggcatcag ctgcggcttc actccatttt cccattgtca gatagactcg ggcacggatt
     3601 cctcttacga catatttatc gaaatgtgat ttgttaacct ttttgatggt acatttgtcg
     3661 agatattcaa acgcagtacc aaggtctgca agaatctgcg cataggtctc ttcaaccgaa
     3721 cttctagcca tagggtccgg cgaaggagta agacgtaaga ttacaccggg ttgagtattg
     3781 ttgcctgcag gattgtatct ttttgcccat gcctgaacaa gctcaaacaa tgcaaatcca
     3841 cgataagcat atgcctcccc aaccgccata tagccgtatt gttccgaacc gggcatgccg
     3901 gcatcataag cttcaattac tttgttggca ttatttatta ccctatagaa taatttccag
     3961 taataattga gatcattgta tttgtgagag agatcacggt gatgagtcag ctgtgcgcaa
     4021 aatttgtaca tcacattgtc gtaggtccat accacatcat cagtcataaa ggcgagatga
     4081 aggcagaaag tttgataacc tccctgtcca ttccaggagg tagtagtgcc gctggagttc
     4141 ccttcgtaca tcatacggtg aattccgtta atggccatca tagcattttc tgtactacta
     4201 aagatactgg cctcatcaac tgaagaggta ggtgctgtgt ccagaaactc tttgctgcaa
     4261 gaccagaagg tcagtactgc agctatcata aatatgatag gatgtattcg tttcatatca
     4321 agtaagcttt acagagtaat attaacacca agtgaaatgg tccttgaagg actgaagcga
     4381 tctccgccgg agtcataatt actcaaagaa tagtttgagt tgagacccct acgggcattg
     4441 aatacataga gatggtctcc tgacaaatat acctgaatct tactaagctt gagcctttca
     4501 caccacttct taggaagtga ataagccagg ttcagactat ttatctccag catattggta
     4561 gtggtaagcc atcttgttga acgtgcagcg tggatatttg aagcgtaagt ggcgttactg
     4621 gtcaacatag gcacattagt cacatcaccc ggcttacgcc acatattggc ggcaaggtcg
     4681 ggtgagaggt ttaccgagtt ggtgctcaca agagcaccta tcatcacatt ttcataagcc
     4741 cgatcatagg tccatccgcc aagttgatag tataaatcaa gcgataaagt gaagcctttc
     4801 cagctaaggg aggtatttat tcctccgaat aggtctggta ttgaggaacc actataatct
     4861 tcacgcgcct tagccatatt tgtcgtataa ttaataccgt taacactcac aatatcctcg
     4921 gaggtatcgc taataaattc gtctgcaatc tcaaagatgt tcaaacctgt ctgaggatcc
     4981 acgcctgccc attgataaag ccaccattca tatacggagt gtccctgttc cactttattg
     5041 taggaagcgc ttctcaagaa gggctcgata ggcagcgagg taatgcggtt tttaaggtat
     5101 gtaccggtaa gtccgacatt ccaatttata tcgccggtct tgaaaatgtc atagtcaagt
     5161 tggaattcaa ttcctctgtt gtagaggccg ccggcatttt cgttgtgaga agtaagaccg
     5221 gttgaaggag gaagggttac ctccatcaaa aggttagtgg tcctgcggtc aaaatattct
     5281 aacgatcccc tcaaccgcct gttgaatagg gagaattcta ctgcaacgtc ccagtttgta
     5341 ctaacctccc attgtagatc gggatttcct gtagaggtca tgctctgggt ataaccgggc
     5401 tcgtccacat tctggttaat cacataaagt gccatccagg gaaagtagct gccaaggtca
     5461 tcacttccaa cagttccaac agaagcacgc agtttcagtc cgtttatcca gtcaatacct
     5521 cgcatgaagt tttctctgtc aagaacccag gatcctccaa ctgaccagaa attgccccaa
     5581 cgtgaatttc tcgcgaatct ggaactgcca tccctgcgga acgaactgga gaagaaatac
     5641 ttgccgtcat agtcataatt ggcacgagca aaataacctt cagtattgta aacgttttta
     5701 tacgaactgg gttgtgcact cacttcgata tagttatcga attcataatt gtctcctatg
     5761 acaatctgat aacgcatcga aaccgaggag ttgagtgaat atgtgtaact gttgctttcg
     5821 tgaccgagca atgcatccac atgatgtttc ccgaagttgt gtccccaggt tagaagttgg
     5881 ttgaaagtcc aggtacgggt attcgagatt gttttagaag ttgcgggggt atttgatgtc
     5941 aactccgaat agaaaggagt agctgaatgt gacacatagt tactgttgta gagagcggca
     6001 tttgccgtga acttgaaatc ctttaggaac gaaatctcaa tgtagggttt cacatccatc
     6061 attgttctct cttgttggtt gaatcgatcc ttaagttgtc tgggggcatt tactcccgaa
     6121 tagatcacac ggcccggaat agtcagtcct gaagctgtag tgtaaccttc tccaaaatca
     6181 taaatcggat taccatgttc atccaatata taagcacctg tgttatcgtg tagatgtaca
     6241 ccatacacgg gaggcatacg tcgcgcaatc atgaatacat tacccattgt tccctgcgaa
     6301 gtggactgaa taccatattg tgtactcaac gtagaagcca tattgactcc gatattgagc
     6361 cagtcactgg cttttgtaga gacattcgcc ctagctgtgt aacgttcaaa tccggatcct
     6421 acaatatacc cttcattgtc ggaatatcct acggaagcat agtaactatt cttgccgtga
     6481 cgtccgtttg cacttaccga ataatcctga acaagtccca cctgctctat tccatttctc
     6541 cagtctgtat catccaacca tttgaaagtc gcgctgctat tgtatttgcc gtttatatcg
     6601 acaacttcgg taataccgca agaccaagga ttgtattctt cagtgaaatt aaggctggtt
     6661 gcaacagagt tagaggctcg acgagctgca tcttcgggag tgacgccatt gagaatgtgg
     6721 ttgttgtaat atagtcgcca ataggtctcc atataatcct ttatgcccat agtttcatag
     6781 tccttaaact cgcgagtcac ccagcctgtg gagactttaa cattgatagt ggctttctcg
     6841 cttttgccct gcttggtagt aaccattatc acaccattgg cggcacgtgc accgtacagt
     6901 gcagtggaag cggcatcctt cagcacagta atactttcga tatcacctga agggatactt
     6961 gagagtactc cggagtagat aataccgtcc ataactataa gcggatcaat attaccattt
     7021 atggatccta tgccacggat agtaatggtg gcatcagcgc ccggttgacc aagggtggag
     7081 gtaagtcgga taccgggtgt cgtaccgtta agagtgttga gcgggttagt attaggtatt
     7141 agctgaatct tttcaccgct aattttgccc gcggatcccg taaatgaagt tttggtagcg
     7201 gtaccatagg ccactaccat tacctcttcc aaggcttcag aatcgggaat gagaaataca
     7261 tctatttcgg ctcgtccgtt tacagggatt tcctggttga ggtaccctat ggaagaaaaa
     7321 ataagtacgc tgttacgcgg taccgatatg gaatatttgc cgtcggcgtc agccgcgact
     7381 ccggaagttg tgccctttag aacaacgctt gcaaaaggta ccggatcgtg attctcatcg
     7441 aatatggtac ccttgacgga aatgtttccg ccttgcccaa gcatccttaa aggcaagaat
     7501 aacagcaata ccattactgt tatcaggaga tgtttgtaat tcatatatag aagtttttag
     7561 gagatttcaa agaaataaaa tatactctcc caccccttca tgccggcaat ttgtaaaaaa
     7621 atataattag tagggttcaa aatactctct tatccatagt aaagtatcgg aaatactaaa
     7681 aacagaacaa gagagaagaa taagatggcc gtttggcgca tcctgaaaca ataaaaatac
     7741 ttattgcaaa aagacatggc ttgcaatatc ttagttcttt tacaaagata agttttttat
     7801 acaaaaaaag agcaacaaat tgctcttttg atgtaactat tttttcaagg gttcatgaca
     7861 ccaacataat ggggccataa gtggtctgta gtagcaggac aaaccgacat ttcatccttt
     7921 gaattttttg acaataacct taccttccaa ggatgacttg tatttgatgg taaaccttaa
     7981 atactcacct ttgttattac agaaaatcaa tatgtactcg gtattagagg atagcgggcc
     8041 caacgtgtac tccaaatcgt aatcacagat acatttgcat tgaggatctg tttcagtttc
     8101 aacaatggtg atggtgtttc cttccacaga gagatctgat tttatagtac ccggacagca
     8161 gttgaatact gcattgacgt gcttgatacg aagatacccg tcacctatcg ctctatattc
     8221 gatatattct tccttatcag ctgattttgt gagattggtc acaatctctt tacatccttg
     8281 atatgaaaaa tctttaattt gaaggttttc attcggtgtc tctctatcgc agcccgttcc
     8341 tcctatcagg aagaacaaag caagcattgt tattatggcc gattttgttt tcatagtggt
     8401 tattttattt attgtaaact ctttcacatg accagatggt gccaacaagc ttgacttttt
     8461 caaaaaattc atcccagcct tccggtattg gtccttctac tccataaaat aagagctgaa
     8521 ctccgtcgca cgaatatact gattccgaaa atatacaaga aggcgagcgc ccgggaataa
     8581 agtccgattt gctctcaatt acaaaataat tggcgttagt ttcaagatct tgatataaag
     8641 aaatttttat gaaatatgcc tttgaatgct cagcacaata atttttaatc cactccatat
     8701 ttcttagcgg atcttttact ccacatacgg ttatatcgtg gttttcataa atttcatatt
     8761 gaggcctctt ttcacaccct gacacaaaaa atagcgtaaa gaggaaacca agaatcagaa
     8821 tagtaatcat tttcattttc actgctaaaa atggtttaaa ttttgacaac attgaatata
     8881 acttttgctg ttttagccaa tttttcaaca acaatcccgt aatgaaaatg gggatcgggt
     8941 tgtgttgcat caccccctat ctctacgaaa aaaataaaat cgtcatcctt atttttataa
     9001 aaagcataat taaggtgcgc cagagatgga agaccgtcgc atcctatcag caaagtttgt
     9061 gtagaaaaat caataccctt taaatcagga tatttatcta tctcatcttg actaaacaga
     9121 gccaataact cctgtctcga ttggaccacc tttccttctc cctgcttcat ttttccatcc
     9181 agaagataca ttttaggcaa ttccttaatg ataattgcat cattagtagt aaggtttttt
     9241 tcttcgcatc ctgttcctcc tatcaggaag aacaggaaaa taacacttaa taaaatcgac
     9301 tttgtcttca tatcctttta gtgctgaaca gtaattctac cctccaaaga tgggctgtac
     9361 ttgataatta actttgaaaa cacataagca ccactgctaa aaatcaatat atactcttta
     9421 ttggaggtta aggggccaaa cgtatattcc aaatcataat tacaaataca atcacatata
     9481 ggatttgttt cactctccac aatagtaatg atgttgtcct tcatagaaac atctgcttga
     9541 atagtgtcag gacagcagtt gaatacggca ttgatatgct tgatatgaag atatccgttg
     9601 gctactgctt tatattcgat agactcttca ccgtgatatg aggttaggag acttttagtt
     9661 tctttacatc cttgatatga aaagtcttta atccgaagac tttcattcga tgtttcctta
     9721 tcgcagcccg ctcctcctat cagaaaacac agagcaagca ttgtaattaa aaccgttttc
     9781 gttttcatga tttacagtat actgagattt ataaatataa caacttctat ctaatatata
     9841 cgcatttact cacgaaatac ttccataact gattaaaaat tgtgcgacaa gagatcctat
     9901 attgtataga tgcaaaaatt agcccaaacg ctgcatacaa tgagattaat cttcttaatc
     9961 ccaattggtt gcattatctg aaagttccct tacaaagata gctttttaaa acaaaaaagg
    10021 agcggttgct cgctcctttt gtcgggatac tccgactcga acggaggacc ccttggtccc
    10081 aaaccaagtg cgctaccaac tgcgccacat cccgaaatca tcccttaatg gggggacaaa
    10141 gataaccttt tttttatact tgctgaaata ttttgctaaa acggcaaaat tgcataactt
    10201 tgcactccca acggtgaggt gggtgagtgg ctgaaaccat cagtttgcta aactgacgta
    10261 cgggcaaccg tatcgggggt tcgaatcccc ctctcaccgc acaacaaaaa aaacagcgcc
    10321 agaaatgctc ccggcgctgt ttttttactt gtatatatct cttaactatt tattatcagc
    10381 ttcaaccttt gcgctgttga attaagttta ataaaacgat cgaaatcgct ttcaaaaagt
    10441 ctgtcctgca ctattctgta tttttcccac tctgtttccg catgtttacg tgccacttca
    10501 gcagaaatag tgcctggatt tgtaagcaat tcatgttcag ttgccataag aatgcgatca
    10561 agatgtgtag cccaatcttg catggtcata ggtatattac gattt
//
LOCUS       DAOSIE010000020         9683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_6788_length_9683_cov_4.129622, whole genome shotgun sequence.
ACCESSION   DAOSIE010000020 DAOSIE010000000
VERSION     DAOSIE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6788_length_9683_cov_4.129622"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1067
                     /locus_tag="PLI69_01360"
     CDS             24..1067
                     /locus_tag="PLI69_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41171.1"
                     /translation="MKIKLFTFTLLALLVANCTREHLPEYGGPENGFGETVTISVTIS
                     PETRVAYDGGVGNNVLSWENGDKLLLAGYDGATYKGSRIFNWDSGNTFTGQEVTGATT
                     YKAYYPGEKITLDANGNVIPGSATSWNQAQNGENSTAHLRNHLLLFDEEANLLTETFN
                     LTLKNSIIKFKLKNVPAGMGTLNSLSWTVETATGVFKAMTLSVTGVTFSSALDTITAF
                     LAFDPAVMKIAANGNFKITLFGNKSYQWSIDVAGGKNYAVGNRYTATVNGGWSEMITP
                     LVYVAESNVNPAGNGFVTDLTACNVSGYFNWNAAKAINISGYHLPSINEWRSIIPQNT
                     NYVYFIIILPRTS"
     gene            1088..2080
                     /locus_tag="PLI69_01365"
     CDS             1088..2080
                     /locus_tag="PLI69_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41172.1"
                     /translation="MHSDYKNTGNNLTYALRYKGTDYVSAWKYEYISDGNNTHMKITS
                     RSVAPSVTIGNISDNNGAFWNTGQENDVVRYFPASGYKSSSGSLFYVGTHGRFWSSTE
                     NNSDNTVAWNMQFHETFAATNSDNNSKDTGQTVRLFAGNAPAPTPLIYVAESNVNSAG
                     TGFVTNLTACDLGNYINWNVAQTINISGYHLPSVDEWRGIIPQNSNYVYFTSNPSYNN
                     ISESVIVQGESINMTSDYKNTGSSNNPAPSYALRYKGTKWVSAWKYEFISDGNNTHMK
                     ITSRRVAPSVTIDHIANANYWSSNNENDVVRYFPASGYKSSTGKTYNVGTYGYY"
     gene            complement(2465..3205)
                     /locus_tag="PLI69_01370"
     CDS             complement(2465..3205)
                     /locus_tag="PLI69_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOG41173.1"
                     /translation="MTLLIIEDEIPARMQLTRLLRKYFPEIEIVGCLDSIVTSQEWLS
                     SNPAPDIILMDVELSDGKCFELFNRVNITSKVIITTAYEDYALPAFKTKCIDYLLKPI
                     GESEFRASMERCIVFCQAEEALESAEEAFEPDYRTKFTIKIGNHILIADVHDIAFFFS
                     ENKATYLVTTDRKQYLLDSSLESLEQQLNPKNFFRLSRSCITSLAAISNISKHFNSRL
                     RVTLVPDVIEPVLVSRARAPLLMQWLDR"
     gene            complement(3218..4264)
                     /locus_tag="PLI69_01375"
     CDS             complement(3218..4264)
                     /locus_tag="PLI69_01375"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOG41174.1"
                     /translation="MMEMARKESRYYNLAAVVILALIVFAARMIFEYLLPFFGQGTGQ
                     SFFFTLKGMLRNYPLTLLMLIADFMLVRSLVNYYSYGHSFLSRTLWELGGVLFIAFVS
                     AILMQLTSSEYLVGDTLNQQLFFSFVVALFFNILVTVVIDIFSYLRWKRRRELAKEVR
                     LRSQANYQYQLMKAQLNPHFLFNSLNVLDYLIHEDQERASDYVKKLSDLYRYQLGLES
                     RQTVILEDELDFVMLYVGLIKERFGEALEVRIDVDRRWLRSRIVPSSIQILIENAVKH
                     NIVKISEPLVIDVRVENEHLVVSNRINLKIKSGDSAGVGLENIDKQYRILFDSHIIVD
                     DSDGFFTVKIPLFI"
     gene            complement(4261..4644)
                     /locus_tag="PLI69_01380"
     CDS             complement(4261..4644)
                     /locus_tag="PLI69_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41175.1"
                     /translation="MGALFKRSGNGRRDFGKANLIIFLFALAHALICYLLYDTSVGDG
                     LFLTILTIAMVFCLIRFYGSPLDVFLGLAFLSCFAGFYFGTSVGDYLHKLFPALGVVN
                     NMIVTFVTTEILGLATVLVVARKNG"
     gene            4736..6265
                     /locus_tag="PLI69_01385"
     CDS             4736..6265
                     /locus_tag="PLI69_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOG41176.1"
                     /translation="MKRLTLILVALMWLPLASFAQNNLSQEDISNIESYIEQIRTDWQ
                     IPGMGVSIQKDGELLLCKGFGVKNLNNPGEMVDPHTLFQIGSVTKSFTAYLLATLVEE
                     GLISWDDTVKEILPDFKLYDPWITEHLQVKDLTSHRTGIGRQVGTYIANLGYDREDIY
                     KMMELIKPSYTFRGAYQYNNITFIPAAMIIEKVTGKSWEDNIRERIFEPLGMTESTIN
                     GEGFAEARNVAHPYEFLSKEGKMVINPLYGEEQALWWLTVIGPAGSVCCPPTDLIKWA
                     QFQLDGGKVGDKQLISKENMDYLHRGITITSQSESKTTLYGHCWFIEQSKKGRLFFHT
                     GTTWGMTTLCAFVPELNLALTIQVNSEAPSEPRYAVLRRVIDLCLDLEDYDYNADFLK
                     EWYSNATKKAEEEAKAAAEKVVEPAPESKLLVGKYTKDPLFGDAWVTLEKGKLYIKVG
                     KQGWKHEMTHVSGNTYKFRSDGTGFEIKFKFEEGRKKAASFEIDFKQGEEFGPWTRLS
                     K"
     gene            6282..7073
                     /locus_tag="PLI69_01390"
     CDS             6282..7073
                     /locus_tag="PLI69_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOG41177.1"
                     /translation="MKRIPTFLFVLLFAGSLFAKSPIIGISSYRSGSSHMIGHTYVNS
                     VRLAGGVPLIIPVTTDDAQIEAILNVIDGLIMTGGEDFDPLKWYGEEPLRANGEIAPE
                     RDEFDVKLIRAAVAKGIPVLGICRGQQLMSVAFGGSLYQDIPSQIASSYVKHRQGDTP
                     GYYGTHTINIEKGSILAEVVGSNKAVVNSFHHQAIKAMPAGFKVTARSADGIIEGIER
                     VSPLKGYADGGALIFGTQFHPEIFTNAGNPLFLGLFEKLVSEASK"
     gene            complement(7080..8435)
                     /locus_tag="PLI69_01395"
     CDS             complement(7080..8435)
                     /locus_tag="PLI69_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOG41178.1"
                     /translation="MGAFHAYDIRGIYNVDFDKYLAYKVGYFIPVLLEADKVLVGRDA
                     RTSSPEIFEYVVKGINDAGADVYDVGLSTTPMVYFGTAIHGFPASVQITASHNPKEYN
                     GMKISRENALPVGFDTGLSTIKRWIEEGRETPVATTRGKIVPFNISEEYISFLNSRKE
                     DVSDLKISIDISNGMAALFARKIFGEGPFYLFDELDGTFPNHDPNPLVPENLIELQKS
                     FITQRSDVGVIFDGDADRVMFVDERGKFISPDLIIALLGHYFFENEKYKGAQRIVLQD
                     IRSSKSVAEYLEPMGAEVVTWKVGRAFAAHKLREIDGLFGGELAGHYYFKDFFYSDSG
                     ILAAILALNVIARFKREGVSLSQLIERINGYFSSGEINFAISKKEDAMVAVRELFVSQ
                     ERPVASYDFDGFRVEFPQWWFNIRPSNTEPYLRFICEATSIDLLEEKVAQAREVIKNF
                     I"
     gene            complement(8441..8998)
                     /gene="def"
                     /locus_tag="PLI69_01400"
     CDS             complement(8441..8998)
                     /gene="def"
                     /locus_tag="PLI69_01400"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOG41179.1"
                     /translation="MILPIYLYGSQVLREKAVEADIEEREELTKLLDDMYETMKQADG
                     CGLAAPQVGRSIRVLIVDGSDLADRYPELADFMRKMINPVFTFKSEEMSTYSEGCLSI
                     PDVDAEIDRPKVVRIKYIDENFQEKEEEFDGFASRMLQHEMDHLDGVVFTDRAAPIRK
                     KILGSKLNNISKGNVATSYRVKIEK"
     gene            complement(9015..9437)
                     /gene="ruvX"
                     /locus_tag="PLI69_01405"
     CDS             complement(9015..9437)
                     /gene="ruvX"
                     /locus_tag="PLI69_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOG41180.1"
                     /translation="MNRIIGIDYGSKRTGLAVSDPLRIFASALDTVPSAKIIDYLQNY
                     LQNQKVELFVVGFPLNMDNTDSQSAPLVQAFINLLKKKFPSIPIVLEDERFTSVLAHR
                     AMIEGGMKKSERRDKSSVDKISAAIILQSYLDRTQKQQ"
     gene            9457..>9683
                     /locus_tag="PLI69_01410"
     CDS             9457..>9683
                     /locus_tag="PLI69_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41181.1"
                     /translation="MARQKEKHGKKLRFSIINDDSHKELFSFRTSKINIILISILVLL
                     AIMAGTYLLTSRTFIRQTIPGYPSPETRRMAV"
BASE COUNT         2754 a         2287 c         2127 g         2515 t
ORIGIN      
        1 cagccgttta aaaacacact gttatgaaaa ttaaactatt tacttttaca ctgctcgctc
       61 tgttggtggc gaactgtact cgtgaacatc ttcccgaata cggggggccc gaaaatggat
      121 ttggagaaac tgttacgata agtgtgacta tctcaccgga aacccgtgtg gcgtatgacg
      181 gtggagtcgg caataacgta ctctcatggg agaatggcga taagctcctg cttgccggat
      241 atgacggcgc aacctataaa ggaagccgga tctttaactg ggattcaggc aacactttta
      301 cgggacaaga ggtaacaggc gcaacaacct acaaagccta ctatcccgga gaaaaaatca
      361 cgctggatgc gaacggaaac gtgatacccg gttcagcaac ttcatggaat caggcacaga
      421 acggagagaa ttcgactgca cacctccgca accaccttct tctgttcgac gaggaggcta
      481 acctccttac tgagaccttt aacttaacct tgaagaatag cataatcaaa tttaaactca
      541 aaaacgtgcc ggccggtatg ggaacgctca atagcctgag ctggactgtt gagactgcaa
      601 cgggagtatt caaagccatg acacttagtg tgaccggtgt tactttctcc tctgctctcg
      661 acaccattac cgctttcctc gcatttgacc ccgccgtgat gaaaatcgct gcaaacggaa
      721 attttaaaat aacgctgttc ggcaacaaat cttaccagtg gagcatcgac gttgcgggcg
      781 ggaagaacta tgccgtagga aatcgctaca ctgcaaccgt aaacggcggt tggagtgaaa
      841 tgatcacccc gctcgtctac gttgccgagt ccaacgtcaa tcctgccggt aacggttttg
      901 tgactgattt aactgcttgc aatgtgagcg gttacttcaa ttggaatgct gcaaaagcca
      961 tcaacatttc cggttaccac ctgccgagca taaatgagtg gcgaagtatc ataccacaga
     1021 acactaatta cgtttacttt ataataatat tgccgagaac gtcatagttc agggtgtgag
     1081 cataaatatg cacagcgatt ataaaaatac cgggaacaat cttacttatg ctcttcgtta
     1141 caagggtact gattatgtct ctgcatggaa gtacgagtat atcagcgatg gcaataatac
     1201 tcatatgaaa attacttctc gcagtgtagc gccatccgtg accattggca acatcagtga
     1261 caacaacggt gccttttgga ataccggtca ggaaaacgac gttgttcgtt atttcccagc
     1321 tagcggctat aaatcaagct caggctcgtt attctacgta ggcactcacg gtcgcttctg
     1381 gtcgtccacg gaaaacaata gtgataatac cgtagcgtgg aacatgcaat tccatgaaac
     1441 cttcgcggct actaattccg acaacaatag taaggatact gggcaaacgg ttcgtttgtt
     1501 cgcaggcaat gcacctgctc ctaccccgct catctacgtt gccgagtcta acgtgaatag
     1561 tgccggtacc ggttttgtaa cgaatttaac tgcttgtgat ctgggtaact atattaattg
     1621 gaatgttgcg caaactatca acatttccgg ataccacctg ccgagcgtag atgagtggcg
     1681 agggatcata ccgcagaaca gcaattacgt ttacttcaca agtaatcctt cttataataa
     1741 tatttccgag agcgtcatag ttcagggtga gagcataaat atgaccagcg actataaaaa
     1801 taccggaagc agtaataatc cggctccctc ttatgctctt cgttacaagg gaaccaagtg
     1861 ggtctctgct tggaaatatg aatttatcag cgatggcaat aatactcata tgaaaattac
     1921 ttctcgcagg gtagcgccat ccgtgaccat tgatcatatc gccaatgcga actactggag
     1981 tagcaacaac gagaacgacg ttgttcgtta tttcccggct agcggctata aatcaagcac
     2041 cggtaaaaca tacaacgtag gcacttacgg ctactactga tcgtctacgg aaaacaatag
     2101 tgataatacc gtagcgtgga acatgcaatt ctacagaatc ttcgcgaaca caaacaacac
     2161 aagatctaag tcaaatgggc taatggttcg cctgttcgct ccgggaaatt aatcaggcat
     2221 attgcatgat ttttacggct taagccttgt ttggggcttt ttcgccccaa tgattggagg
     2281 aaactctaac gccctctttc gatagataga tctctctcac ctacgacgac aatgaaccct
     2341 gaaagttgaa taaaaaactt tcagggtttt gtttttgtga cttggaagca acccgtaacc
     2401 tgcaacccgt accccacacc actccacact acaaattaca cactacatac tacacactac
     2461 tcactcacct atccaaccac tgcatcagca gcggcgctct ggcacgggaa acgagtaccg
     2521 gctcaataac atccggtaca agagtcaccc tcaatcttga gttgaagtgt ttggagatat
     2581 tgctgattgc ggccaaactg gtaatgcagc ttctcgaaag tctgaagaaa tttttaggat
     2641 tgagttgttg ctccagggat tcgaggctgg agtccaacag gtattgcttc cgatcggtag
     2701 ttacaagata ggtggccttg ttttccgaaa agaaaaatgc tatgtcgtgt acatcggcaa
     2761 ttaggatatg gttgccaatt tttatggtga attttgtacg gtaatcgggt tcgaaggcct
     2821 cttcggcact ttccagcgcc tcctcggcct gacaaaatac aatgcagcgt tccattgagg
     2881 ccctaaactc actttcaccg ataggtttga gcagataatc tatgcatttt gttttaaagg
     2941 caggaagtgc atagtcttca taggcggtgg ttataattac cttggaggtg atgttaaccc
     3001 tgttgaaaag ttcaaagcat ttgccgtccg agagctctac atccatcaat ataatatccg
     3061 gagcgggatt cgaacttagc cactcctgcg aagtgacgat cgagtcgaga cagccgacta
     3121 tctcgatttc gggaaaatat ttgcgcaaga ggcgggtaag ctgcatccgg gcagggattt
     3181 catcttctat gatcagcagg gtcatattgg cgtaacttta tatgaagaga gggattttta
     3241 ccgtaaaaaa accatccgag tcatcaacga ttatatgaga gtcaaataga atcctgtatt
     3301 gcttgtctat attttccagt cccactccgg ctgagtctcc cgatttgatc ttgagattta
     3361 ttctattgga gaccaccaga tgttcatttt ccaccctcac atctatcacc agcggctctg
     3421 aaatcttgac tatattatgt tttactgcat tctcaattag aatctggata ctgctgggga
     3481 ctatcctgct tcggagccat cttctgtcta catctatcct cacttccaga gcctctccga
     3541 agcgctcctt tatcaacccc acatagagca ttacaaagtc gagttcatcc tccagaatta
     3601 ctgtctggcg cgactccaaa ccgagctggt agcgatagag atccgagagt ttcttgacat
     3661 aatcggaggc gcgctcctga tcctcgtgga taaggtagtc gagtacattg aggctattga
     3721 agagaaaatg tgggttgagt tgcgccttca tcagttggta ttggtaattg gcctgactgc
     3781 gaagcctaac ctctttagcg agctcacgtc ttcttttcca gcggaggtag gaaaaaatat
     3841 ctatcactac cgttaccaat atattgaaga aaagcgccac tacaaaggag aaaaagagct
     3901 gctgattgag agtatccccc acaagatatt ccgatgaagt gagttgcata aggattgcag
     3961 atacgaaggc tataaaaagc acccctccca gctcccaaag agtacgtgaa aggaaggagt
     4021 ggccgtagga ataatagttt accaagctgc gcaccagcat aaaatccgca ataagcatca
     4081 atagggtcag cggataattt ctcaacatac ccttgagggt aaaaaagaag gattggcctg
     4141 tcccttggcc aaagaatgga agcaagtatt caaatatcat ccttgcagca aatacaatca
     4201 gcgccaggat gaccaccgca gcaagattgt aatatctgct ctccttgcgc gccatctcca
     4261 tcatccgttt ttacgggcta caaccagtac cgtggcaagt cccaggattt ccgtagtgac
     4321 aaaagtcaca atcatattgt taactacacc caaagcggga aataacttgt gtagataatc
     4381 tcccacagat gtcccaaaat agaatccggc aaaacagctg agaaaagcca gaccgaggaa
     4441 tacatcgagg gggctgccat agaaacgaat cagacagaat accatggcta tggtgagaat
     4501 agtgagaaac agaccatctc ccactgaggt atcgtagagc agataacata tcaacgcatg
     4561 tgcaagagca aagaggaata ttatgagatt ggccttgcca aaatccctcc tgccattgcc
     4621 gctcctttta aataatgcgc ccatatcgtg attaattcgt taataattgt aaatataggt
     4681 atttttttcc aaataaaaga tggctatatt tgtcctccgt aacaaactat ctgtgatgaa
     4741 acgattgaca ctcattcttg tagcccttat gtggctacca cttgcctctt ttgcgcagaa
     4801 caatctctcg caagaggata tttccaacat cgaatcctat atcgaacaga tccgcaccga
     4861 ctggcaaata cccggtatgg gcgtttccat ccaaaaggat ggagaacttc tgctctgcaa
     4921 aggatttgga gtcaaaaacc tcaacaatcc cggggaaatg gtggatcccc atacactatt
     4981 ccagattggg tcggtcacca aatcctttac tgcatacctg ctggctactc tggtggaaga
     5041 ggggcttatc agctgggatg ataccgtaaa agagatactt ccggacttca aactgtacga
     5101 cccctggata acggaacacc tacaggtcaa ggacctgacc tctcaccgga caggtatcgg
     5161 gagacaggta ggcacctaca ttgccaatct tggctatgac agggaggata tctacaaaat
     5221 gatggaactc atcaagcctt catacacctt cagaggcgct taccaatata ataacattac
     5281 cttcattccc gctgccatga ttatcgagaa ggtgaccggt aaaagctggg aagataatat
     5341 ccgcgaacga attttcgagc ccctcggaat gaccgaatcc accatcaacg gtgagggatt
     5401 tgcggaggcc cggaatgtgg ctcatcccta cgaattcctc tccaaagagg gtaaaatggt
     5461 tataaatccc ctctacggtg aagagcaggc actctggtgg cttactgtca taggacccgc
     5521 cggatccgtc tgctgccctc ccacagacct gattaaatgg gcacaatttc aacttgacgg
     5581 tggaaaagtg ggtgataagc aactgatttc caaagaaaat atggattacc tccacagagg
     5641 gataaccatt acctcccaaa gcgaatccaa aaccaccctc tacggccact gctggtttat
     5701 cgaacagagt aagaaaggac gcttgttttt tcatacaggc accacctggg ggatgactac
     5761 attgtgtgca tttgtgccgg aactcaacct cgcacttacc atccaagtca actctgaagc
     5821 gccgagtgag cctcgctatg cggttttacg ccgagtgata gacctctgcc tggaccttga
     5881 agattacgac tacaatgccg atttcctgaa agagtggtat tccaatgcca ctaaaaaggc
     5941 cgaagaagag gcgaaagctg ctgcggaaaa agtggtggaa cccgcaccgg agtcgaagct
     6001 gctcgtaggc aaatatacca aagatcctct atttggggat gcctgggtaa ctcttgagaa
     6061 gggaaaactc tatatcaagg taggtaaaca gggctggaaa cacgagatga cacatgtaag
     6121 cggcaatacc tataagttcc gctccgacgg caccggtttt gagataaaat tcaaattcga
     6181 agagggacgg aaaaaagctg cttcttttga aattgacttc aagcagggtg aagagttcgg
     6241 tccctggacc agattaagta aataacaaaa acactcacta tatgaaacgc attccaactt
     6301 ttttgtttgt actgcttttt gcaggctcac ttttcgccaa gtcccccatt atcggtattt
     6361 cttcatacag gagcgggtct tcacacatga tagggcacac ctatgtaaac tccgttcgtt
     6421 tggcaggtgg tgtacctctg atcatacccg ttactactga tgatgcgcag atagaggcta
     6481 ttctcaatgt aattgacggt ctgatcatga cgggcggcga agactttgac ccgctgaagt
     6541 ggtatggtga agagcctttg agggccaatg gtgaaatcgc ccctgaaagg gatgagtttg
     6601 acgttaaact aatcagggcg gctgtagcca aaggtattcc cgtgctggga atatgtcgcg
     6661 gccagcaatt gatgagtgta gcttttggcg gctcgctata ccaggatatt ccctcacaga
     6721 ttgcaagcag ttatgtcaag cacagacaag gagatactcc cggttattat ggcacacata
     6781 ccatcaacat agagaagggg tccatactgg ctgaagttgt cggcagcaat aaagccgttg
     6841 tcaattcatt ccaccatcag gccataaaag cgatgcccgc agggtttaag gtgactgcaa
     6901 ggtctgctga tggaataata gagggaattg aaagggtatc tcccctcaag ggatatgctg
     6961 acggaggtgc acttattttc ggaacacaat tccaccccga aattttcact aatgccggca
     7021 atcccctctt cctcggactt ttcgagaagc tggtttccga agcctcgaaa tagttctgat
     7081 taaataaaat tcttaattac ttcgcgggct tgggcgactt tctcttcgag aaggtctatt
     7141 gatgtggctt cgcagataaa gcgaaggtaa ggttcggtat tggagggacg tatattgaac
     7201 caccattggg ggaattccac tctaaaaccg tcaaaatcgt atgatgcaac cggacgctcc
     7261 tgagagacaa acaactctct tacagctacc atcgcatcct cttttttgga tatggcaaag
     7321 ttgatttcac cggagctgaa atagccattt atcctctcta tcagctgtga cagcgacaca
     7381 ccttctctct tgaagcgcgc gatgacattt agcgccagaa tggctgccaa aataccgcta
     7441 tccgaataga aaaagtcctt gaagtagtaa tggccggcca gttcaccacc gaataggcca
     7501 tctatctcac gcaatttatg ggcggcaaaa gccctgccaa ccttccaggt aacgacctct
     7561 gctcccattg gttcaaggta ctcggcaacc gacttggaac tgcgtatgtc ctgaagcaca
     7621 atacgctgcg cccccttata tttctcattt tcgaagaaat agtgacccag aagtgctata
     7681 atcaaatcgg gtgaaatgaa tttacctctc tcatccacaa acatcaccct gtccgcatca
     7741 ccatcaaata ttactcccac gtcgctgcgc tgagttataa acgacttctg aagttcgatg
     7801 agattttcgg gtacgagcgg atttggatca tggttgggga atgtgccgtc cagctcatcg
     7861 aagagataga aaggcccttc tccgaaaatt tttctcgcaa aaagagccgc cataccattg
     7921 gatatgtcaa tactgatttt caaatccgaa acatcctctt tcctggagtt caaaaaggag
     7981 atatactcct ctgaaatgtt aaacgggact attttgcctc gcgtggttgc aacaggtgtc
     8041 tccctaccct cttcaatcca tctctttata gtagaaagac ctgtatcaaa gccaaccgga
     8101 agagcatttt cacgagaaat tttcataccg ttgtactctt tgggattatg agaagcggta
     8161 atctgcaccg aggcagggaa accgtgaatt gcagtgccga aatagaccat cggcgtggta
     8221 ctcagcccga catcgtaaac atccgcaccg gcatcgttga tacctttaac cacatattcg
     8281 aaaatctcag gggaagatgt tcttgcatcc ctacccacca atactttatc ggcttccagt
     8341 agaacgggaa taaaataacc taccttataa gccaggtatt tatcgaaatc cacattatag
     8401 attccgcgta tatcataagc gtgaaatgct cccataaagt ttacttttct atttttaccc
     8461 tgtaagatgt tgcgacgttt cctttggaga tgttgttgag tttgctccct agaatttttt
     8521 tacgaatagg cgcagccctg tcagtaaaga ccactccgtc gagatggtcc atctcatgct
     8581 gtaacattct ggaggcgaat ccgtcaaatt cctcctcctt ctcctgaaag ttttcgtcaa
     8641 tatatttgat gcgtaccact ttgggtctgt caatctctgc atcgacatcc gggatactca
     8701 aacaaccttc actataggta ctcatctctt cgcttttgaa ggtaaaaacg ggatttatca
     8761 tcttgcgcat gaaatcggca agttcgggat agcgatcagc caaatcactg ccgtccacaa
     8821 tcagaaccct gattgaacgg cctacttgtg gagctgcaag gccacaacca tcggcttgct
     8881 tcatggtttc atacatgtca tccagaagct tcgtcagctc ctctctctcc tcaatatccg
     8941 cctccacagc cttttcacgc aatacctggg atccatataa ataaatgggt aatatcatat
     9001 ctgtaaattt ttaatcattg ttgtttctgt gttctatcta aataactttg caggatgatg
     9061 gcagcactga tcttatccac ggaggatttg tcacgacgtt ccgatttttt cattccaccc
     9121 tctatcattg ctctgtgtgc caggactgaa gtaaatcgtt cgtcttcaag cacaatggga
     9181 attgagggga actttttctt cagcaggttg atgaaagcct gtaccagcgg agctgattga
     9241 gaatcggtgt tgtccatatt taaagggaag cccaccacaa agagttccac cttttgattt
     9301 tgaaggtaat tttgaagata atctattatc tttgcggacg gaacagtatc gagggctgac
     9361 gcaaatatgc gcaacgggtc gctcactgcg agaccagtgc gcttgctgcc gtagtctatg
     9421 cctattatcc tgttcatgat gcaaagttaa tgttttatgg cacgtcaaaa agaaaaacat
     9481 ggtaaaaagt tgagattttc gataataaac gatgactctc acaaggagct tttctcgttt
     9541 agaacatcaa aaatcaacat tatactgatc tctatactgg tattgctggc aataatggca
     9601 ggcacttacc tgctcacttc acgtactttt ataagacaga ctattcccgg ttatccttca
     9661 cctgaaacca gacgaatggc ggt
//
LOCUS       DAOSIE010000021         9603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_6858_length_9603_cov_4.772099, whole genome shotgun sequence.
ACCESSION   DAOSIE010000021 DAOSIE010000000
VERSION     DAOSIE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9603
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6858_length_9603_cov_4.772099"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1938
                     /gene="uvrC"
                     /locus_tag="PLI69_01415"
     CDS             130..1938
                     /gene="uvrC"
                     /locus_tag="PLI69_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOG41182.1"
                     /translation="MEVRDKIEFLPQTPGVYRYLDSEGTVIYVGKAKNLKKRVSQYFA
                     PSESLDRKTRVMVSKIADLEYTVVGSEQDALLLENLLIKEFQPRYNIILKDGKTYPWI
                     SIRNEPFPRITTTRRYVKDGSLYFGPYSSAFHANQLTQLISGLYKLRDCKLSLTPEKI
                     AAGKHRPCLNYHIGKCAAPCVGLIREEEYNRQIEGITSLLKGDFSSLKREFKEKMSLH
                     SQHLEFEKAQEYKEKLHLLEQYYSKSVIVGFGGKDIDAISFVEEGSEVFSNFIRVRQG
                     AIVHSFNMELKVRIEQSREEILSEIIAEIISRFGELSQEVLVPFLPDQKFEGKNIHIP
                     VRGDKKSLLELSRKNATQMKLNRLKQEEALRPEEHRERIVANLKKDLGLKELPLHIEC
                     FDNSNIQGTDPVAACVVFREGVPSKKEYRHFNIKSVVGANDYASMKEVVNRRYSRMIA
                     EGESLPQLVLIDGGRGQANFAYEALEELGIAGEVAVAGIAERLEELIIPGDPTPLWLD
                     KNSTSLRLLMQIRDEAHRFGLTHHRGKRSKRQIRSELSQIKGVGEATQTKLLKHFRSV
                     ARIRQASFDELKECVGPHLATLIFGYFHSSQDGSIE"
     gene            complement(1892..2590)
                     /gene="pnuC"
                     /locus_tag="PLI69_01420"
     CDS             complement(1892..2590)
                     /gene="pnuC"
                     /locus_tag="PLI69_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOG41183.1"
                     /translation="MTNTPALKLSWFDWFLILGVTATSIITSAFAENFDWLGLIINIT
                     GIINLVLCAKGNLINYFFGIIYNALYVYVAFSTKLYADSAIYLLYYLPMQFVGWANWK
                     KNQSKESGAVITRRITMKSAALLLAIAAVLVPLFAWILSLPAIGDAQPWADSATTVAS
                     IIAMYMMVKAYVEQWYIWLLLDLIQSVKWLIETIRGREHAAMMLIMFLFFTANAIYGL
                     IQWSRLEKSENNRK"
     gene            complement(2602..5607)
                     /locus_tag="PLI69_01425"
     CDS             complement(2602..5607)
                     /locus_tag="PLI69_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41184.1"
                     /translation="MRITPRVGPFVLLTILIAALLLSQNRAAAQYYTLGNDPAKAKWS
                     VIQGETYKVIYPQQTDSLARRYLYLFEKYKHTNLVGLKIESPKMPIILHPYTTTSNAT
                     VVWAPKRMEIFTSPPATGGYAQNWETQLALHEGRHLGQMQHYTKGVFSFFNILFGEQS
                     IALGIGFYPSVWLLEGDAVLNETDFSNAGRGRSGEFLMYYRTAFLQNDIRSYDHWRYG
                     SYQYFAPNKYAFGYMLTSMMRYASGNYHATGDAMSVQVKRWWDFFGVWNTSFIRATGR
                     TPRKNWRLTVSFMSDVWQKDYLSRGPYHIAEPLLAKEPRIYTNYTDILPTNRGIIATK
                     SGYHDSRTLVSIDSFGKERRIRPFSYTTGNIISDGDGTLIWAETVPDLRWELQNFSII
                     RSYNLNNGKIMDITSRTKYFNPALSTDGQSLFVVEYPVGGGSRLVQIEKESGDVTKSI
                     PAPQGGQITEIAPIGDSLYALIITELGMGLYWTELSKGDGVWYNEIPDQHRYIQDLTV
                     SGSSLCFTSDLDGLVNSYIYNPKLKLLRKLTNSRFGAKYPHIDNKVGRLYWADFGLKG
                     YQPVSAPLDSLDWRVADFEKPFVNDVAEHLSRQARLNTEPLSEKEDFALKTKIDSIPA
                     KEYKKFPHLLRIHSWAPFYANINRIMNMSYEHFYQLVSAGATVISQNDLSTATMQLGY
                     SYHKGFHSGHLNFNYSGLYPVLEIAVDYNDRYRTNTTIEEAHHNQTIMRSDPAMLPGD
                     MYPIRYTIDTTDIPSWDVSLRTYVPLNFSKSGWNSGLIPQLKYDFSNDKYSLFGHKSI
                     YRHSLTYGVRYYKVLPNPKSRLFPKWGFGVQLSGSYSHGPHNSSGNIAYIYTYGYLPG
                     VLRNQGIKLSAAYQRQFNKRAFGYLPNLASMPRGFSSIVLMDYAKFTADYAIPIYLGD
                     VTWPWLYYLKRLQIIPFGDFAINNASWHPQNGVIKPVDHKYLFSYGVDVLVNAHLLRI
                     GSELSFGFRYARTSLGTNYWNALFSTGLF"
     gene            complement(5659..6618)
                     /locus_tag="PLI69_01430"
     CDS             complement(5659..6618)
                     /locus_tag="PLI69_01430"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HOG41185.1"
                     /translation="MTKQSLIALLLASLLLLSACKDRAEVPARAFPMPQVPAMLGSNM
                     EQASQYIALHFWDRLFAPGIEYSKDSSLVMGVTYADFEEAFAKYLTTLDNVPLPVMNV
                     AQDTMLARAAGHKLSHPESRIWETITELSGKYLYDPNSPYRNEEMFLPVLAHLKESPF
                     TTDEEKVHYSHLYRLCSYNRIGTPAANFKYTLKKGNTSTLYQIKAEYVLLFFSNPECQ
                     SCKEIVDILANLPEISQLIDQKRLAVLNIYPDTELDEWYEYLPHYPETWINGYDQDLV
                     LNMQTIYNIRAIPSLYLLDKEKKVIIKDAPPEKIISRLVRIAG"
     gene            6742..7014
                     /locus_tag="PLI69_01435"
     CDS             6742..7014
                     /locus_tag="PLI69_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOG41186.1"
                     /translation="MNIKPLADRVLIEPKEAETKTAGGLFIPDTAKEKPQQGIVVAVG
                     NGKKDEPMELTVGDTVLYGKYAGTEISVDGKDYLIMRQSDVVAVLK"
     gene            7034..8665
                     /gene="groL"
                     /locus_tag="PLI69_01440"
     CDS             7034..8665
                     /gene="groL"
                     /locus_tag="PLI69_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694639.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOG41187.1"
                     /translation="MAKEIKFDIEARNLIKEGADALANAVKVTLGPKGRNVVIDKKYG
                     APQVTKDGVTVAREIELEDRFANMGAQMVKEVASQTNDQAGDGTTTATVLAQSIINVG
                     LKNVTAGANPMDLKRGIDKAVAVVVESLKSQTTPVGDDYSKIEQVGTISANNDNAIGK
                     LIADAMSKVKKDGVITVEEAKGTTTEVKLVEGMQFDRGYISAYFMTNPDKMEAVLENP
                     YILITDKKISTMKDLLPVLEPIARDGKSLLIIAEDVEGEALTTLVVNRLRGTIKIAAV
                     KAPGFGDRRKEMLQDIAILTGGAVISEERGFTLENATVAMLGQAEKVVIDKENTTIVN
                     GAGKKGQIDDRANQIRKQIETTTSDYDREKLQERLGKLAGGVAVLYVGAATEVEMKEK
                     KDRVEDALNATRAAVEEGIIPGGGVAFIRATSALKGLKGDNDDETTGINIIARAIEEP
                     IRMICENAGKEGSVIVQKVKEGKGDYGYNAHTDTFENFFSTGVIDPTKVMRVALENAA
                     SVAGMLLTTECALADIKEELPPMPMGGGAPGMGMM"
     gene            8740..9174
                     /locus_tag="PLI69_01445"
     CDS             8740..9174
                     /locus_tag="PLI69_01445"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOG41188.1"
                     /translation="MDDIRYMREALKEAKEALKQGEVPIGGIVVCKGHIIARGHNLTE
                     LLTDPTAHAEMQLITMATESIGGKYLTDCTLYVTIEPCPMCAAALAWAQLGRLVYGAD
                     DPKRGYSLFAPSMLHPKTEVTKGVLAPECGNLVADFFRDKRS"
     gene            9254..>9603
                     /locus_tag="PLI69_01450"
     CDS             9254..>9603
                     /locus_tag="PLI69_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOG41189.1"
                     /translation="MEWLEGLGLLGLFLGTFMAATIVPFSSDVLYIGMLLTGVNPWIV
                     WLVGTLGNWSGGLTSYGVGYIGKWEWIERWFKVKPETLAAQKVKIDRWGPPLALVTWL
                     PFIGDVFARALGFY"
BASE COUNT         2619 a         2068 c         2416 g         2500 t
ORIGIN      
        1 ccaatcggac ccttcatctt tatgactttc attatgtccg atttattgat tacttttgtt
       61 cgttccattg gctggcgtag tttaagtttt ccacaaatat acatattttg ctcaaatcct
      121 gctcaaaaaa tggaagtcag ggataaaata gaatttcttc cgcaaacccc gggtgtctac
      181 aggtaccttg attcagaagg gacagtaata tatgtgggca aggcaaagaa cttaaaaaaa
      241 agggtttcgc aatattttgc gccgtcggaa tcactcgatc gcaagacaag ggtaatggtc
      301 tcaaaaattg ccgatctgga gtataccgta gtcggaagcg aacaggatgc gcttttgctt
      361 gagaatttac ttataaagga gttccagccc agatataata ttatcctgaa ggatggaaaa
      421 acctatccat ggatctctat cagaaatgaa ccattcccta ggataactac taccaggaga
      481 tatgtcaagg atggatcttt gtactttgga ccatacagtt ccgcatttca tgcaaatcag
      541 ctgacacaac tgatttccgg cctttataaa cttcgcgatt gcaaactttc ccttactccg
      601 gaaaaaattg ccgcaggcaa acatcgtccc tgcctcaact accatatagg caaatgtgcc
      661 gcaccttgtg tagggctgat cagggaggag gagtacaatc gtcagattga gggcatcact
      721 tctctgctca aaggtgattt ttcctcactt aagagggaat ttaaggaaaa aatgagcctc
      781 cattcacaac atctggagtt tgagaaggcg caggagtata aagagaagct tcacctgctt
      841 gagcaatatt atagcaaatc ggtgatagtg ggattcgggg gcaaggatat agatgctatt
      901 tcttttgtag aagaggggag cgaagtcttt tccaacttca tccgtgtccg acaaggtgct
      961 attgtacact ccttcaacat ggagctcaag gtgcgtatag aacagagtag ggaggagatt
     1021 ctgagtgaaa ttatagcaga aatcatttcc aggttcgggg aactttcaca ggaagtgctg
     1081 gtgcctttct tgcccgatca gaaatttgag ggcaaaaata tccatatacc tgttagagga
     1141 gataaaaaat cattgcttga gcttagccgc aaaaatgcca ctcagatgaa gctcaaccgc
     1201 ctcaagcagg aggaggcttt aagacccgaa gagcaccggg agcgcatagt ggccaatctt
     1261 aaaaaggatt tagggcttaa ggagcttccc ctccatatag aatgctttga caactccaat
     1321 attcaaggga ccgatcccgt tgctgcatgt gttgtcttca gagagggcgt tccaagcaaa
     1381 aaagagtatc gtcatttcaa catcaaaagt gtagtcgggg ccaatgacta tgcttcaatg
     1441 aaagaggtgg ttaaccgtcg ctattcgcgt atgatcgcag aaggcgaatc tcttccacag
     1501 ctggtgctga tagatggagg gaggggacag gccaactttg cctacgaggc actggaagag
     1561 cttggtatcg cgggagaagt ggcagtggcg ggcattgccg aaagattgga agaacttata
     1621 atcccgggag acccgacacc gctctggctt gacaaaaact ccacctcctt gcgcttgttg
     1681 atgcaaatac gcgacgaagc acacagattc ggtttgacac atcacagggg aaaacgtagc
     1741 aaacggcaga tccgcagcga gctttcacag atcaaagggg tgggagaagc tacacagaca
     1801 aaactcctta aacatttcag aagtgtggca cgcatccgcc aggcttcatt tgatgagctc
     1861 aaagagtgtg tcggccctca tcttgcaaca ctcattttcg gttattttca ctcttctcaa
     1921 gacggctcca ttgaatgaga ccatagatgg catttgctgt gaaaaataga aacattatca
     1981 gcatcattgc agcatgttct cttccacgta tagtctctat aagccatttt acactctgaa
     2041 tcaaatcaag caaaagccag atgtaccatt gttccacata ggctttgacc atcatataca
     2101 tagctattat tgaagctacg gtagtagcgg agtcggccca cggctgggca tctccgatgg
     2161 caggcaatga gaggatccat gcaaataacg gcacaagcac cgcggctata gccaaaagta
     2221 gggcagcgct cttcatggtg atccgcctgg tgattaccgc tccactctct ttgctttggt
     2281 ttttcttcca gtttgcccat cctacaaact gcataggcag atagtagagc agatatatgg
     2341 cagaatcggc atatagtttt gtggaaaacg ctacgtagac atatagtgca ttgtagatga
     2401 tgccgaagaa gtagtttatc agattgcctt tggcgcaaag taccagattg atgatacccg
     2461 tgatgtttat gataagccca agccagtcga aattctcagc aaaagctgat gtgatgatac
     2521 ttgttgcagt gactcccaat atcagaaacc agtcgaacca ggataatttg agtgcgggtg
     2581 tatttgtcat tgggtttagt ttcaaaagag acctgtgctg aagagtgcat tccagtaatt
     2641 agtaccgaga cttgttctgg catagcgaaa tccaaacgaa agttcgctac ctatacgcag
     2701 aagatgggca tttaccaata catctactcc gtatgaaaag agatacttgt gatcaacggg
     2761 ctttataaca ccattctgag gatgccagga agcattattg atggcaaagt caccgaaagg
     2821 aattatctgt aggcgtttga gatagtataa ccagggccag gtaacatctc cgagatagat
     2881 tgggatggca taatcggctg taaattttgc ataatccatc agcacgatac tgctgaaacc
     2941 acgcggcatt gaggccagat tggggagata tccaaaagct ctcttattga actgtctctg
     3001 atatgcagcc gagagcttga tgccctgatt gcgcaaaaca ccgggaagat aaccgtaagt
     3061 gtagatatat gcaatgtttc cggaggaatt gtgtggtccg tgagaatagg atccgctgag
     3121 ttgcacgccg aaaccccatt tggggaagag acggctcttg ggattgggca gcaccttata
     3181 gtagcgcact ccataggtca aactatggcg atagatgctt ttgtgtccga aaagagagta
     3241 tttgtcgttg ctgaagtcat atttcaactg aggtataagg ccgctattcc atcccgactt
     3301 gctgaagttg agagggacgt atgttctcaa tgagacatcc catgagggaa tgtccgtagt
     3361 atctatagtg tatcttatgg gatacatatc tccgggaagc atagcgggat cggatctcat
     3421 tatagtctga ttgtggtgtg cctcctcaat ggtggtgttg gtacggtatc tgtcgttata
     3481 gtccactgca atttccaata ccggatagag tcccgagtag ttgaagttta ggtgtccgga
     3541 gtggaatccc ttgtgatatg agtatccgag ctgcattgtg gcagtactta ggtcgttttg
     3601 tgaaatgacc gttgcgccgg ctgacaccag ctgatagaaa tgctcatagc tcatattcat
     3661 aatcctgttt atattggcgt agaaaggagc ccaggagtga attcttagaa ggtgcggaaa
     3721 ttttttgtac tcttttgcgg gtatggagtc tatttttgtc ttgagcgcga aatcttcctt
     3781 ttcggagagg ggttccgtgt tgagcctggc ctgacgagac agatgttctg ccacatcatt
     3841 tacaaagggt ttttcaaagt ctgccactct ccagtcaagc gagtcaagag gtgccgatac
     3901 gggctggtag cctttgagtc cgaagtccgc ccagtagagt cgtccgactt tattgtcgat
     3961 atgagggtat ttggcgccga atcgcgaatt ggtgagtttg cgcaataatt ttagcttggg
     4021 gttgtaaata taactgttta caagaccatc cagatcagag gtgaaacaga gactgctgcc
     4081 gctaacggtc agatcctgta tgtatctatg ctgatcgggt atttcgttgt accatactcc
     4141 atcgcctttt gagagctccg tccaatagag ccccattccc aattctgtta tgatcagcgc
     4201 gtagagcgaa tctccaatcg gtgctatctc cgtaatttgt cctccttgag gagcgggaat
     4261 gctttttgtc acatctccgc tttctttttc gatctgaacc aggcgggaac ctcctccgac
     4321 cggatattcc acgacaaaga gggattgtcc gtcagtcgag agtgccggat tgaagtactt
     4381 agttcgggac gtgatgtcca ttatttttcc attgttgagg ttgtagctgc gtatgatgga
     4441 gaaattttgc agctcccagc gcaggtcggg cacagtctct gcccagatga gggtgccgtc
     4501 tccatcagat atgatattgc cggtagtgta cgagaagggt cttatcctgc gttctttacc
     4561 gaaagagtca atggagacaa gtgttctgga gtcatgatac ccgcttttag tcgcaatgat
     4621 tcccctattt gtcggaagaa tgtcggtata attggtgtat atgcgtggtt ctttggcaag
     4681 caacggctcc gctatgtgat agggccctct gctcagataa tctttctgcc atacatcact
     4741 catgaatgag accgttagac gccagttttt cctcggagta cgccccgtag ccctgatgaa
     4801 actggtgttc cagacgccaa agaagtccca ccacctttta acctggaccg acatcgcatc
     4861 gcctgtagca tgataatttc cggaggcata tctcatcata ctggtcagca tataaccgaa
     4921 tgcatattta ttgggagcaa aatattgata tgaaccatat ctccaatggt cataggaacg
     4981 aatgtcattt tgaagaaatg ccgttctgta atacattaaa aactccccgc tacgaccgcg
     5041 tccggcgttg gaaaagtcgg tctcgttgag gaccgcatcg ccttcgagaa gccataccga
     5101 agggtaaaaa cctattccca gtgctatcga ctgttctccg aagaggatgt tgaagaaact
     5161 gaatacgcct ttggtgtagt gctgcatctg cccaaggtgc cgtccctcgt gaagagccag
     5221 ctgtgtctcc caattctgag cgtatccccc tgtcgccggt ggcgaggtaa atatttccat
     5281 tctctttggg gcccaaacta cggtggcatt actcgttgtg gtgtaggggt ggaggattat
     5341 tggcatttta ggggactcta tcttgagtcc gaccagattg gtatgcttat atttctcgaa
     5401 aagatagagg tacctccttg ccagagaatc ggtctgctgc gggtatatga ctttgtatgt
     5461 ttctccctgt atgacagacc attttgcctt cgcagggtca ttgcccagcg tataatactg
     5521 cgctgctgcc cgattttgtg acaacaaaag ggctgcaatg agtattgtca ggaggacaaa
     5581 tgggcctacg cggggtgtaa ttctcataaa tagggctcaa agatagcaat tttgcaacaa
     5641 atacttgaaa aacaggcatt agcctgcaat cctaaccagc cgtgagatta ttttctcggg
     5701 tggagcatcc ttaataataa ctttcttctc tttatcgagg agatagagtg aaggtatcgc
     5761 acggatgtta tagatggtct gcatatttag taccaaatcc tgatcgtaac catttatcca
     5821 ggtttcggga tagtgtggca ggtattcata ccattcatcc aactcagtat cggggtagat
     5881 gttgagtacc gccagccttt tttggtcgat aagttgtgaa atttcgggca aattggcgag
     5941 aatatccact atctccttgc aactttgaca ttcgggattg ctaaaaaaca gcagtacata
     6001 ttctgctttt atttggtata aagtgcttgt gttgcccttt tttaaggtgt atttgaaatt
     6061 tgccgccggt gtgccaattc ggttgtaaga gcaaaggcgg tacaggtggc tatagtgaac
     6121 tttctcttca tcggtggtga agggggactc tttcaaatga gccaacacgg ggagaaacat
     6181 ctcttcattt ctgtagggag aattggggtc ataaagatac ttcccggaaa gttcggtaat
     6241 ggtttcccaa atcctactct caggatgaga gagcttgtgt ccggctgcac gggcaagcat
     6301 tgtatcctga gcgacattca ttacgggaag aggtacattg tccagcgttg taagatattt
     6361 tgcaaaagcc tcttcaaaat cggcgtatgt gactcccatt accagagaac tatctttgga
     6421 gtattcgatt cccggggcga agagcctatc ccagaaatgc agagcgatat attgggatgc
     6481 ctgctccata ttgctcccga gcattgctgg tacttgtggc atggggaatg ctctggccgg
     6541 cacttcagct ctatctttac acgcggaaag cagtagaagc gatgctaaaa ggagagctat
     6601 gagtgattgt tttgtcatcg tacaccaatt tttttgcaaa tgtaaagaaa atagtgtgac
     6661 aaattggcag aaatatagcg atggcatcta ttttgccgaa aatagagcat aagattttat
     6721 aaaattaaaa catttcatac tatgaatatt aaaccattag cagacagagt tctgattgaa
     6781 cctaaagagg cggaaaccaa gaccgcgggc ggattgttca ttccggacac cgctaaagaa
     6841 aagccccagc agggcattgt agtagccgta ggtaacggta aaaaagacga acccatggag
     6901 ctcaccgtgg gtgataccgt attgtacggc aaatatgccg gcactgaaat cagtgttgac
     6961 ggtaaagatt atctaataat gcgtcagtca gacgtagtcg cagtacttaa ataattaaag
     7021 aaatttccat atcatggcaa aagaaatcaa atttgacata gaagcacgta atctcatcaa
     7081 agagggtgca gatgctttgg ccaacgcagt gaaggtcact ctcggaccta aaggccgcaa
     7141 cgtagttatt gacaagaaat atggtgctcc ccaggtaacc aaggatggag tcaccgtagc
     7201 cagagaaatt gaacttgagg acagattcgc caatatgggc gctcagatgg ttaaagaggt
     7261 agcctctcag accaacgacc aggccggtga cggtaccact accgctacgg ttctcgctca
     7321 gtccatcatc aacgtaggac ttaagaatgt taccgcaggc gcaaatccca tggacctcaa
     7381 gcgaggtatc gataaagcgg ttgccgttgt tgtggagagc ctcaagtcgc agaccactcc
     7441 cgtaggtgac gattactcaa agatcgaaca agtcggaacc atctccgcca acaatgacaa
     7501 cgccattggc aagctcatcg cagacgcgat gtccaaagtt aagaaagatg gcgtcattac
     7561 tgttgaggag gcaaaaggta ccactaccga agtaaaattg gtagagggaa tgcaatttga
     7621 cagaggctat atttcagcct acttcatgac taaccccgac aagatggagg cagtgcttga
     7681 gaatccctac attctcatca ccgataagaa aatttccacc atgaaggatc tccttcccgt
     7741 gctcgagccc atagcgagag acggcaaatc tctgctcatc atagcagagg atgtggaagg
     7801 cgaagcactt accacccttg tggtcaacag actacgcggc accatcaaga ttgctgcagt
     7861 aaaggcaccg ggcttcggtg accgtcgcaa agagatgctt caggatatag caatccttac
     7921 agggggagcc gtaatttccg aggagagagg gtttactctc gagaatgcaa ccgtagcaat
     7981 gctcggacag gctgaaaaag tagtaatcga caaggagaat accaccattg tcaatggcgc
     8041 cggcaagaaa ggacagattg acgaccgcgc caatcagatc cgcaagcaga tcgaaactac
     8101 gacatccgac tatgaccgtg aaaagctcca ggagcgtctg ggtaagcttg cgggcggtgt
     8161 agcagtactt tatgttggtg ccgcaaccga ggttgagatg aaggagaaga aagatagagt
     8221 tgaggatgct ctcaatgcta cccgtgctgc agttgaagag ggtattatcc ccggtggtgg
     8281 cgtagccttt atcagagcta cttcagcact caagggcctc aagggtgaca atgatgacga
     8341 aaccacaggt atcaatatca ttgcacgtgc cattgaggag cccatcagaa tgatttgtga
     8401 aaacgcgggt aaagagggta gcgtaattgt acagaaggtt aaggaaggta aaggtgacta
     8461 cggttataat gcccacaccg acacttttga gaacttcttc agtacaggcg taatcgaccc
     8521 cacaaaggtt atgcgcgtgg ctcttgagaa tgcagcttca gtggccggaa tgttgctcac
     8581 taccgagtgt gcactcgccg atatcaagga ggagctgcct ccgatgccaa tgggcggtgg
     8641 tgctcccggt atgggaatga tgtaatcccg tatattggat agaaatatta tatttgcggg
     8701 gagcaatcat atgtggttgc tcccctttta tttcagacaa tggatgatat caggtatatg
     8761 cgcgaagcgc taaaggaggc taaagaggca cttaaacagg gagaggtgcc cataggaggg
     8821 attgtggtat gtaagggcca cataattgcg cgcggccaca atcttaccga acttctcacg
     8881 gatcctaccg cccatgcgga gatgcagttg atcaccatgg ctaccgaatc tattggtggc
     8941 aaatatctga ccgactgtac tctttatgtg actatagagc cttgccccat gtgcgccgca
     9001 gcgctggctt gggcgcaact tggccgcctt gtttatggag ccgacgatcc taagaggggt
     9061 tattcgctct ttgctccttc gatgctacat cctaagacag aggtgactaa aggggttctt
     9121 gctcccgagt gtggcaatct ggtagccgac ttcttcagag acaaacgaag ctgatgtttg
     9181 gttgcgattt acaagtaaag ttaagagcca aaggccaacg gctaacggct aatggcaatt
     9241 tcaagtattg agtatggaat ggcttgaagg acttggtctt ctgggactgt ttctgggtac
     9301 atttatggca gccaccattg tgccttttag ttcggacgtc ctatacatag gaatgcttct
     9361 taccggagta aatccttgga tagtgtggct ggtcggcaca ctgggtaact ggtcgggagg
     9421 cctgacctcg tatggtgtcg gatatatagg caaatgggag tggatagaga ggtggtttaa
     9481 ggtgaaaccc gagactctcg ctgcgcaaaa agtaaagata gatcgctggg ggccgcctct
     9541 ggcccttgtt acctggcttc catttatagg agacgtcttt gcaagagcac tcggttttta
     9601 taa
//
LOCUS       DAOSIE010000022         9279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_7091_length_9279_cov_3.979402, whole genome shotgun sequence.
ACCESSION   DAOSIE010000022 DAOSIE010000000
VERSION     DAOSIE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7091_length_9279_cov_3.979402"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLI69_01455"
     CDS             <1..429
                     /locus_tag="PLI69_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOG41190.1"
                     /translation="CGGGRYDDLTGIFGLPNMSGVGISFGADRIYDVLKGLDLFPQEM
                     ASASTVLFSNMGEDEVAFSVPVAALLRRAGIACEIYPDNTKLRKQFEYADKKGIPYLA
                     IVGANEIAEHSVTLKNLTTGEQQLVKIPEIIDKLNQVLNV"
     gene            461..533
                     /locus_tag="PLI69_01460"
     tRNA            461..533
                     /locus_tag="PLI69_01460"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:494..496,aa:Met,seq:cat)
     gene            complement(772..1914)
                     /locus_tag="PLI69_01465"
     CDS             complement(772..1914)
                     /locus_tag="PLI69_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG41191.1"
                     /translation="MNKLAKYIIIAVTVALVGFLVWFFSEIVWYLLISAVIFLIGKPI
                     TTILTSFSIKRFRIPNWLASAITLLAIFSAVGLVLFFLTPIVIRIISNVGQLDFERVL
                     TNISEPFIKFNYRLTTLFPSLGEDFRVEVYILDQIKESTSITFFTRAFSSIASFTVDL
                     AIGIFSVVFISFFFLQERNLMGNMLNAIVPDKYEDNMKRALSSAENLLRRYFTGIFIE
                     SILITILNTLGLYFIVGFDFDLAVMVAFASGVLNVIPYVGPLTGELLGVLAGIVTHYS
                     TPDAGPVLPFVLLIFGVMLTTQLIDNYLFQPFIYSSSVKAHPLEIFIVILVAGYVGGV
                     IGMLVAIPAYTVIRVFAGEFLSHFKIVQRLTSSLKRGGDNVSDEAS"
     gene            complement(2005..2382)
                     /locus_tag="PLI69_01470"
     CDS             complement(2005..2382)
                     /locus_tag="PLI69_01470"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOG41192.1"
                     /translation="MYYEDFDNAGQKERPGDDVYSKPVRAGKRTYFFDVKTTKGNDYY
                     LTITESKRRVEKDGRFVYDKHKIFLYKEDFEKFSEGLQEVVDFIKTKCPVIETQNEAR
                     GDESDEEQQSGPASFTDVNFDEL"
     gene            2470..3420
                     /locus_tag="PLI69_01475"
     CDS             2470..3420
                     /locus_tag="PLI69_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HOG41193.1"
                     /translation="MLTRRLLRVKAFKVLFSNMAQESPSIDNMKKELRYSCEKTRELY
                     WFLLSICKPLVNVARERIEAGKRKYKATPEEANPNYRFVNNSFVELLSRDEEMDKFCK
                     KRGLVWSDYDYTVKRIFRSISASDYYKEYMASTESSFERDCNLFIDIFSNEFEDNEEL
                     AGILEERSIYWLDDITFVLDEIIRNIGVMRSTRRNISPVQKEEDIQYALQLLEESFLR
                     YQEYSALIGENIVNWDVDRLVSTDKTIIIMGITEAVVFETIPVKVTINEYVEISKYFG
                     TPNSRIFVNGILDSIILKKLNEGEIVKKGRGLQEGSELKK"
     gene            3441..3764
                     /gene="yajC"
                     /locus_tag="PLI69_01480"
     CDS             3441..3764
                     /gene="yajC"
                     /locus_tag="PLI69_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOG41194.1"
                     /translation="MNVLTFFILQADAAAPKAGGSYTMLIMLALIFVVMWLFMIRPQK
                     KKQKELDEFRNSLKKGDKVITVGGIYGTVDEVKETKILIEVDKDVKIFVDKASLVKDP
                     ADTKQ"
     gene            3774..4754
                     /locus_tag="PLI69_01485"
     CDS             3774..4754
                     /locus_tag="PLI69_01485"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HOG41195.1"
                     /translation="MSGSDNKDFKKRKGRVWVSLILSLLLAFFIWLVHNLSNEYSAFL
                     RFQVKVVTNITGHQPEAVADEILLVRGRATGFYILRNNRRGSVPLITLEVPPELFTPD
                     KELPDRFCLTANNLKDKLSAALSGEIAVDFIETSILTFTFPGQSHRKVPINPLISVVY
                     KKQYTKVGDIVISPDSVLVYGNTEDIANIRSINTLPVNLTNVGKSIQGVTRLETIRDV
                     RIDCDEVRYFIRVDRYVEVSGQMSVKVNNLPSGYSIVLIPSKVKFTARIPFTAKNDNI
                     FDEISLNMDYNLFINQRSSKVIPTLERGDADIYSYSLDPPMIEAVITQER"
     gene            4751..5350
                     /gene="coaE"
                     /locus_tag="PLI69_01490"
     CDS             4751..5350
                     /gene="coaE"
                     /locus_tag="PLI69_01490"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOG41196.1"
                     /translation="MKESSVILCTGGIGSGKSYVVKVFNALDIPSYDSDSRTKLLYDR
                     DRALLESINTIAGGDVVKDGVLQRAAFAAKIFSDPEMLARVESVVHPAFATDFLKWKE
                     EQQSDIVLMESAILLEKPLLLPLIDYVLVVHAPLECRVERVMARDNVSRERVLQRISN
                     QWSDSRRLEMADFSIETNDRDSIIEKVMDIIRKIRDGKR"
     gene            5337..6134
                     /locus_tag="PLI69_01495"
     CDS             5337..6134
                     /locus_tag="PLI69_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOG41197.1"
                     /translation="MGRGKWIGGFLGWVIFGPIGGLIGFLIGSMADMGSKLIESDVNK
                     GRPYTGTHKSDKSYDPRTASSAKERNIGQRNSFIVSLLVLSTAVIKADGKYLKSELDY
                     VREFIRSNFGDRAVPEAMEILKGLKDKEINIYEVGGQIRQYMNYSQRMQLFHYLVALA
                     QSDGNVCQAEIDVLRSIASAMGISSANANSILAMFEKNIDSAYTVLEIDKNATEEEIK
                     RAYKRLAMKHHPDKVATLGPDIQKSAEENFKNIQEAYETIKKERGIN"
     gene            6158..6601
                     /locus_tag="PLI69_01500"
     CDS             6158..6601
                     /locus_tag="PLI69_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOG41198.1"
                     /translation="MEKTDLKKILSVSGQRGLFLYLSQARNGVIVESLETKQRTTFGE
                     SAKVSSLADISVYTTSEDVSLKVIFTDMAKKLNNGPAMSSKEDPKKIKAFFREVLPEY
                     DEDRFYVSHMKKILEWYNLLQKFASLEFLEEEEDEEQEAEVTTSE"
     gene            6626..7948
                     /gene="rimO"
                     /locus_tag="PLI69_01505"
     CDS             6626..7948
                     /gene="rimO"
                     /locus_tag="PLI69_01505"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOG41199.1"
                     /translation="MKKLQFISLGCSKNRVDSEHLMKQLSASGYTLVSESVDLVVEPV
                     DCVVINTCGFIQDAKEESIETIFKVVDAKNNGLANKVFVFGCLSQRYMETLAEMIPEV
                     DGFFGVFDFETLLSALGVKYNSGLLQQRILTTPSHYAYLKISEGCDRSCSYCAIPMIR
                     GPHISIPEEEVLIEARNLAQMGVKELIVIAQDTTYYGLDLYRKRALATLLEKLSLIEG
                     IEWIRLHYSYPAGFPEDVLRLIAENPKICKYIDIPLQHSSDKVLKMMRRSIDGRQTRS
                     LIEKMRQMVPGVVLRTTMLVGHPGEGKREFEDLLEFVSEIRFERLGAFIYSEEEGTFG
                     AQNYKDSVSRKVKQERYERLMELQAGISLEYNRSRIGSKEKVILDSFSDGVYVGRSMS
                     ESPEVDGEILIGASSVDKLFKGKNPVGRFVTVDILNADEYDLVAVCSV"
     gene            complement(8017..8958)
                     /locus_tag="PLI69_01510"
     CDS             complement(8017..8958)
                     /locus_tag="PLI69_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOG41200.1"
                     /translation="MSKDSLPRGGSVWSEGMVLNPFTDFGFKYLFGTEKNKDLPIGFI
                     NIILKEELESPIEEIEFLDKELIPSVETAKRSVVDVLCSTKDGVRYLIEMQNQYYRHM
                     FDRLIYYNCDLISDTVSRGDKKKYGDIKKVFTICLSNFQIEKNGRIKNEYKMCNTKTG
                     KVISSIIHIITLQLPCVNIKNIAKSGKDYESLLVLLKLMTDRKMGITYKEFKEAVDSI
                     DVSDKIKRYFMDLICKAALTSLTEKERQEYRDHYFAVTYDDIIESAREEGLEEGMEKG
                     KAEGIRQVAKSLLSQGMSLESISKATGLSQNEISKLS"
BASE COUNT         2689 a         1903 c         2124 g         2563 t
ORIGIN      
        1 tgtggtggcg gccgctatga tgaccttacc ggtattttcg ggctgcccaa catgtcggga
       61 gttggtatct cctttggagc tgaccgcatc tacgatgtgc tcaaggggct ggatcttttt
      121 cctcaagaaa tggcttcggc ctctacggtt ctcttctcca atatgggaga ggatgaggtg
      181 gctttttccg tgcctgtggc agctttactt cgtcgcgccg gaatagcttg tgagatctat
      241 cccgacaata ccaagctccg caaacagttt gagtatgctg ataagaaggg aattccttac
      301 ctggcaattg tcggagctaa tgaaattgcc gaacattcgg ttacattgaa aaacctgaca
      361 accggagaac agcaacttgt caagattccg gaaatcattg ataaattaaa tcaagttttg
      421 aatgtttgaa aaaaatgctt atctttgtag cccattacac cgcggagtag agcagttggt
      481 agctcgtcgg gctcataacc cggaggtcgg aggttcgagt cctccctccg ctacaaagaa
      541 taaagcgctg acaaaatcag cgcttttctt attatcagga ggactcgaac ctccatcgcc
      601 gccaggcgcc atccccttga ggggtgtcag actcgtctga cggggtgatt atagccctcc
      661 gggtgcgtca gcacccgagt cctccctccg ctacaaagaa taaagcgctg acaaaatcag
      721 cgcttttctt attatcagga ggactcgaac ctccatcgcc gccaggcgcc atcaacttgc
      781 ctcgtcggag acattatccc cgccacgttt cagactgctg gtaaggcgtt ggactatttt
      841 aaaatgagat aaaaattctc cggcaaagac tcttattacg gtatatgccg ggattgcaac
      901 cagcatacct ataactccac ctacataacc tgctacaagt atgactataa aaatctccag
      961 cgggtgtgct ttgacactgc tcgagtagat aaagggctgg aaaagataat tgtcaatcag
     1021 ctgggtagtg agcattactc caaaaatcag caacacaaag ggcaatacag gacctgcgtc
     1081 aggcgtgctg tagtgggtga caattccggc caaaaccccc aacaattcgc cggtcagcgg
     1141 gccgacataa gggatgacat tgagcacacc ggaggcaaag gccaccatta ccgccaggtc
     1201 gaagtcaaac cctacgataa agtagaggcc gagggtattt agtatggtaa tcagaatgga
     1261 ttcaataaaa ataccggtga agtagcgtct caagaggttt tctgctgacg aaagggctcg
     1321 cttcatgttg tcctcatatt tatcgggaac aatcgcatta agcatattac ccatgaggtt
     1381 acgctcctgt aggaagaaga aggagatgaa gaccactgaa aagatgccta tagccagatc
     1441 taccgtaaac gaggcgatcg aggagaatgc acgtgtaaag aaagttatgc tggtactctc
     1501 cttgatctgg tcaaggatat agacctccac tcggaaatct tccccaagag aagggaagag
     1561 tgtggtcaat ctgtaattga actttataaa aggttcggag atgttggtaa gtaccctttc
     1621 aaagtccagc tggccgacat tactaataat ccttattacg ataggagtca gaaagaaaag
     1681 aacgagtcct accgcagaga atatggcaag cagagtgatt gctgaagcga gccagtttgg
     1741 aatgcggaat cttttgatgg aaaaggaggt cagtattgtg gtaatgggct tacctatcag
     1801 aaatataacc gcagaaatca gaagatacca gactatctcg ctaaagaacc atacgagaaa
     1861 tcccaccagt gctacggtta ctgctataat gatatatttt gcgagtttat tcatccgcta
     1921 ttatttaatt tcaaaaatag tcatctataa tttaaaaaac aagccggtac aaagaccggc
     1981 ttgtatgcga tagttcgaaa tatgctacaa ttcatcgaaa ttcacatcag tgaaagaggc
     2041 agggccggac tgttgttcct catctgactc atcgccgcgt gcctcattct gcgtctcaat
     2101 gacggggcat ttggtcttga taaaatcaac aacttcctga agaccttctg agaacttttc
     2161 gaagtcttcc ttatagagga aaatcttgtg tttgtcataa acaaaccttc catccttttc
     2221 tacccttctt ttgctttcag tgatagtgag ataatagtcg ttacctttgg tggtcttgac
     2281 atcaaagaaa tatgtccttt ttcctgccct taccggcttg gaataaacat cgtcgccggg
     2341 gcgctccttc tgtcctgcgt tgtcaaaatc ttcgtagtac atatcaattt ttttaaaatt
     2401 ctttcacaaa gataaatttt tattttgata gtaaaggtta tatttgcaaa atatttaaga
     2461 aatttaagaa tgctgaccag aagattgctc cgtgtaaaag cgtttaaagt tcttttttcc
     2521 aatatggccc aggagtctcc ctccattgac aatatgaaga aagaattgcg ctactcctgt
     2581 gaaaagaccc gggagctcta ctggtttctt ctaagtatat gcaagccgct ggtaaatgtg
     2641 gccagggaga ggattgaagc aggcaagagg aagtacaagg ctacacccga ggaggctaac
     2701 cccaattata ggtttgtgaa taactccttt gtggagttgc tctcccgtga tgaagagatg
     2761 gataaatttt gcaaaaaaag gggtcttgtc tggagcgatt atgactatac ggtcaaaaga
     2821 attttcaggt caatatctgc cagcgattac tacaaggagt atatggcttc gacggagagc
     2881 tcttttgagc gcgactgcaa cctttttatc gatatttttt caaatgaatt cgaagataac
     2941 gaggagttgg cagggatatt agaggagcgt tccatttatt ggttggatga catcacattt
     3001 gtactggacg agattatccg aaatataggc gttatgcgct ctacccgaag gaatatatct
     3061 ccggtgcaaa aagaagagga tatccaatac gcattacagt tgcttgagga gtctttcttg
     3121 cgttatcagg aatactccgc actcattggc gagaatatag ttaactggga tgtggatcgt
     3181 cttgtttcca cagacaagac cattatcatc atgggcatca cggaggcagt ggtattcgag
     3241 actattcccg taaaggtgac catcaatgaa tatgtggaga tttccaaata tttcggaact
     3301 ccgaatagcc gaatatttgt caacggtatt cttgactcca ttatccttaa aaaactcaat
     3361 gaaggagaga ttgtcaagaa gggaaggggc cttcaggaag gcagtgaact gaaaaaataa
     3421 tttatttaat acaattttat atgaacgttt tgacattttt tattttgcag gcagatgctg
     3481 cggctcccaa agcaggaggc agttacacaa tgttaattat gctcgccctt atcttcgtgg
     3541 ttatgtggct cttcatgatc cgtccacaga agaaaaaaca gaaagaactt gacgagtttc
     3601 gcaacagtct taagaagggc gataaagtta ttaccgtggg aggtatttac ggcacggtag
     3661 acgaagttaa agaaaccaaa attctcatcg aggttgataa ggatgtaaaa atcttcgttg
     3721 acaaggcctc tcttgtaaag gatccggcag ataccaagca ataaaatacc attatgtccg
     3781 gtagtgacaa caaagatttt aaaaagcgga aggggagagt gtgggtatct ctgatactct
     3841 ctctcctgct ggcttttttc atctggcttg tccataacct ttccaatgaa tattctgctt
     3901 tcctgcgttt tcaggtaaag gtggttacca atattaccgg tcatcagccg gaagcggtgg
     3961 ccgacgagat tctccttgta agaggaaggg ctaccggttt ctatattctc cgcaacaacc
     4021 gccgagggtc ggtacccctt ataacactgg aagtgcctcc cgaattattt actcccgata
     4081 aagagttgcc ggacagattc tgcctgacag cgaacaatct gaaggacaaa ctctcggctg
     4141 ctctctcagg tgagattgca gtggacttta tcgagacaag tattcttaca tttacatttc
     4201 ccgggcagtc acatcgtaag gtcccgatca accccctaat ttccgtcgtt tataagaagc
     4261 aatacaccaa ggtgggagat attgtcattt ctcccgactc ggtattggtt tacggtaata
     4321 cggaagatat tgcaaacatt cgctctataa acacacttcc tgttaatctg accaatgttg
     4381 gtaagtctat tcagggtgta actcgccttg aaaccatacg agacgtccgt atagattgtg
     4441 atgaggtccg atacttcata agggtggata gatatgtaga agtgtcgggt cagatgagtg
     4501 ttaaggtcaa caacctcccc tccggatatt ctatagtcct gatcccttca aaagtgaagt
     4561 ttaccgcaag aatacccttt acggcgaaga atgataatat atttgacgaa atatcgctca
     4621 atatggacta taatctcttc ataaatcaga ggagttccaa ggtaatccca actctggaaa
     4681 ggggtgatgc ggatatatat tcctactcgc ttgatccacc aatgattgag gctgtaatta
     4741 cacaggaaag atgaaggaga gcagtgtcat attgtgtaca ggcgggatag gcagcggaaa
     4801 gtcttacgtc gtcaaggttt ttaatgccct cgatataccc tcgtatgact ccgattccag
     4861 gacaaaactg ctctatgata gagatagagc gctattggaa agcattaaca ccatagccgg
     4921 aggtgatgtc gtaaaggatg gcgtgcttca gcgtgccgct tttgccgcaa aaattttttc
     4981 agatcccgag atgcttgcaa gagtagaatc agtagtacat cccgcttttg cgacagattt
     5041 tcttaaatgg aaagaggagc aacagagcga tatcgtactg atggagtctg caattttact
     5101 cgaaaaacct ttgctgctcc cgctgataga ttatgttctg gtagttcacg ctcctctcga
     5161 gtgtagggta gaacgtgtta tggcacgtga caatgtctca agagaaaggg tattgcagcg
     5221 tatttccaat caatggagtg atagtcgccg actcgaaatg gctgacttct ccatagaaac
     5281 aaatgatcgt gattctatta tagaaaaagt aatggatatt ataagaaaaa ttagagatgg
     5341 gaagaggtaa gtggatagga ggctttctag gatgggtgat ttttggcccc attggcggac
     5401 tgatcggctt tttgattggt tccatggctg atatgggctc aaaattaatt gagtcggatg
     5461 tcaataaggg acggccatat acgggaacac ataagagtga taagagttat gatccccgta
     5521 ctgcctcctc tgccaaggag cgcaacattg gccagcgcaa cagtttcatt gtaagtctgc
     5581 tggttctctc aactgccgtc atcaaggccg acggcaaata tctcaaaagc gagctcgact
     5641 acgtgaggga gtttatccgt tccaattttg gagatagggc tgtgcctgag gcaatggaga
     5701 tcctcaaggg acttaaggat aaagagatca atatatatga agtaggaggt caaatccgcc
     5761 agtatatgaa ttactcgcag aggatgcagc tttttcacta tctggtggct ttggctcaaa
     5821 gtgacggtaa tgtttgtcag gcagaaatag atgtccttag gagtatagca tcagcaatgg
     5881 gcatctcttc agcgaatgcc aactccatcc tcgctatgtt tgaaaagaac atagattccg
     5941 cctataccgt actcgagata gataagaatg ctaccgaaga ggagatcaag agggcttaca
     6001 aacgtcttgc gatgaagcat caccccgata aggtggccac acttggtccg gatatccaga
     6061 agagtgcaga agagaatttc aagaatatcc aggaggcata cgaaactatc aagaaagagc
     6121 ggggaattaa ttaaaatcga atagttttat aaataatatg gaaaagacag atctaaaaaa
     6181 gattttatcg gtatcgggac agcgcggatt gttcctgtat ctttcacagg ccagaaacgg
     6241 cgtgatagta gagtcattag agaccaaaca gaggacaact ttcggggagt cggccaaagt
     6301 ctcctctttg gcagatattt cggtatatac cacatcagaa gatgtttctc taaaagtgat
     6361 atttaccgac atggctaaaa aactcaataa tggccctgcc atgagttcca aggaggatcc
     6421 caaaaagatc aaagccttct tcagagaggt tcttcccgaa tatgacgagg accgcttcta
     6481 tgtttcacac atgaagaaga tacttgaatg gtataatctg ctccagaagt ttgcctccct
     6541 cgaattttta gaagaggaag aggatgaaga acaggaagca gaagtgacta cttcggagta
     6601 aactcctgcc gaagagtccc aatcaatgaa aaagctgcaa ttcatttcac tcggttgctc
     6661 caaaaacagg gttgattcgg agcatcttat gaaacaactc tctgcatcgg gttatacctt
     6721 ggtgtcggag agcgttgatc ttgtggtgga gccggtagat tgcgttgtaa tcaatacctg
     6781 cggatttatc caagatgcaa aggaagagtc catagagact atatttaagg tggtggatgc
     6841 taagaacaat ggtctcgcca ataaggtatt tgtgtttggt tgcctatcgc agcgttatat
     6901 ggagactctg gcggagatga ttccggaagt ggatggattt ttcggagttt ttgattttga
     6961 aactctcctc tcggctctag gagtaaaata caactcagga ttgttgcagc aaaggatttt
     7021 gactacacct tctcactatg catatttgaa aatttccgaa gggtgcgacc gcagttgctc
     7081 ttattgtgca ataccgatga ttcgcggccc ccatatctcg ataccggaag aggaagtgct
     7141 gatagaggct cgcaatctgg cacagatggg ggttaaggag cttatagtga tagcgcaaga
     7201 cactacctac tatgggcttg atctctacag aaagcgcgct cttgctactc tcctcgagaa
     7261 attatccctt attgagggta tcgagtggat ccgtctccat tattcttatc cggcgggttt
     7321 cccagaagac gtgctccggc tgattgcgga gaatcccaag atctgcaagt atatcgatat
     7381 tccgctccag cactcttctg ataaggtgct gaaaatgatg aggcgctcca ttgatggccg
     7441 ccaaacccgc agtctgattg aaaagatgcg tcaaatggtg cccggagtag tactgcgtac
     7501 caccatgctt gtgggccacc ccggcgaggg taagcgcgaa tttgaggatt tactcgaatt
     7561 tgtgagtgag attcgctttg agcgtctcgg ggcgtttatc tattcggagg aggaggggac
     7621 cttcggtgca caaaactata aggatagtgt cagtcggaag gtcaaacagg agcgttatga
     7681 gcgtctgatg gagcttcagg caggaatctc tttagagtac aacagatcgc gtatcggctc
     7741 gaaagaaaag gtgattttgg attctttctc agatggagtc tatgtgggac gcagtatgag
     7801 cgaatctccg gaggtggatg gcgaaatact catcggagcc tctagtgtgg ataaactttt
     7861 taaaggaaaa aatcccgtcg gccggtttgt cactgtagac atcttaaatg ccgacgaata
     7921 tgatctggtg gcagtgtgta gtgtgtagtg tgtagtggta cggggtgcgg gttacgggtt
     7981 acgggttacg ggggagttct tagtatagag actcaattac gagagtttac tgatttcgtt
     8041 ttgtgataat cccgtagctt ttgagattga ctccaatgac atgccttgag acaacaatga
     8101 ctttgcaact tgacgaatac cttcagcctt acccttctcc attccttcct ctaatccctc
     8161 ttcacgtgcg gattcgataa tatcatcata agtaactgca aaataatgat cacgatattc
     8221 ttgacgctcc ttttcggtaa gtgacgtcaa agctgccttg cagataagat ccatgaagta
     8281 tctcttgatt ttgtctgata cgtcaatgct atctactgct tccttaaatt ctttataagt
     8341 gattcccatt ttcctgtcag tcattagttt tagcaaaact aataaacttt cataatcttt
     8401 cccacttttg gcaatgtttt taatatttac acaggggagc tgtagggtta taatgtgtat
     8461 tatgctgctt attactttcc ctgttttcgt gttgcacatc ttatactcgt tttttatccg
     8521 gccgtttttt tcaatctgaa aattgctcag gcagatggtg aaaaccttct tgatatcgcc
     8581 atactttttc ttgtctccgc gactgacggt atcgcttata agatcgcagt tataatagat
     8641 caatctatca aacatgtgcc tataatactg attctgcatt tctatcaggt aacgtactcc
     8701 atccttggta ctgcatagaa cgtcgacaac cgaccttttt gcagtctcta cggaaggaat
     8761 cagttcctta tccaaaaact caatctcctc tatgggactt tcaagctcct ctttgagaat
     8821 gatgttaata aaaccaatcg gcaaatcctt gtttttctcg gttccaaaaa ggtacttaaa
     8881 accaaaatct gtaaagggat tcaaaaccat tccctccgac cacacgctac ctccacgtgg
     8941 caatgaatct ttgctcataa tgtgataact taaatgttaa gtaaataaaa tatattgtaa
     9001 atgctaaaat aataaattat aatgcaaata aaggaaataa aaacaataaa aataagtaat
     9061 agagacaaaa tttgttcgct attcagaaac aagctgcact gccttccact cgactctaca
     9121 tactcaaatt ctaaaaaata ttttttttat tgaaaataaa atcttatttt gaggcgttta
     9181 gtatcaatcg tttgaagtag gggaacttca taatcggatt tacaatatta aaataatcga
     9241 ctgcataact atgacagccg acatcttaaa aacacatag
//
LOCUS       DAOSIE010000023         9272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_7097_length_9272_cov_3.744494, whole genome shotgun sequence.
ACCESSION   DAOSIE010000023 DAOSIE010000000
VERSION     DAOSIE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9272
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7097_length_9272_cov_3.744494"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLI69_01515"
     CDS             complement(<1..193)
                     /locus_tag="PLI69_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41201.1"
                     /translation="MIGTLHIKWFEETDSTNTQLQNDRHNLENLAVYAAYFQTAGRGQ
                     RGNRWESKRGENLTFSILFK"
     gene            265..654
                     /gene="rsfS"
                     /locus_tag="PLI69_01520"
     CDS             265..654
                     /gene="rsfS"
                     /locus_tag="PLI69_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOG41202.1"
                     /translation="MKKTKEVNKSEIETNLNEINVIADAMMEKKGHDVISLDLRRINT
                     AFTDHFVICNADTPIQVVAISDYVEEMMEKKADRPAVRKQGREHGFWIILDYTDIVVH
                     IFKTEQRLFYRLEDLWADAVKTVFEEV"
     gene            656..2611
                     /gene="ftsH"
                     /locus_tag="PLI69_01525"
     CDS             656..2611
                     /gene="ftsH"
                     /locus_tag="PLI69_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOG41203.1"
                     /translation="MASNTRNTRGGRPTGKPNRRKAPNWLFWFYILLFVGLGYMFLTS
                     SGVEPIKKEWIEVKEEILPSGDIDKVVYIRNERRGDIYISKDSISEYKNIFGGKEPKS
                     GPHFYFLVSDKFDAEVELEAARESLGEGRKFDVVVEEKHNYWGRALEWLMFPLLLILM
                     WFLLFRPMRSNMGGGAGGGIFNVGKSQAKLYEKENNIKITFKDVAGLQEAKVEIMEIV
                     DFLKNPKKYTALGGKIPKGVLLVGPPGTGKTLLAKAVAGEANVPFFSISGSDFVEMFV
                     GVGASRVRDLFKQAKEKAPCIVFIDEIDAVGRARSKNLGYSSNDERENTLNQLLTEMD
                     GFGSNSGVIILAATNRPDILDKALMRAGRFDRQIHLDLPELKERLEIFKVHLRNLKLV
                     SGFDIDFLAKQTPGFSGADIANVCNEAALIAARNNKEFIDRQDFLDAIDRIVGGLERK
                     SKFISPEEKRTIAYHEAGHATVSWLLPNANPLLKVTIIPRGQSLGAAWYLPDERHLTT
                     KEQLKDEMASTIAGRVAEEIINGKISTGALSDLEKLSKQAYAMVTFFGMSDKVGNVSF
                     YDSTGRSEMALSKPYSEKTAELIDSEVKRFIDEAHKTATRILNENREGFEQLAELLLE
                     KEVIFSEDLEKIFGPRKGGKDPNKLLA"
     gene            2615..3496
                     /locus_tag="PLI69_01530"
     CDS             2615..3496
                     /locus_tag="PLI69_01530"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOG41204.1"
                     /translation="MSSLSQRITSGIIFFIVVAGALLCSCTFLPLTVAIGALMMSEYY
                     SMTLAKRFFAEQILMILAATLAFVLFFLTFKIDLNHGYIFLVFIPVLAACISFIYTST
                     RDLRRGEADRGVSRDIVNSVAHLMMPALYIVAPLLLAQFLVFGLKGVYTPWLLLSVMI
                     LIWLNDVGAYMFGMLLGQRQGSRKLFPSVSPKKSWAGFLGGALITFLISILLYYVTGS
                     LSLFYISWWHWLAMAAIVSVFGLFGDLFESLIKRACNVKDSGNIMPGHGGLLDRFDAT
                     LFVIPAIVLYLKLVCVI"
     gene            3534..4187
                     /locus_tag="PLI69_01535"
     CDS             3534..4187
                     /locus_tag="PLI69_01535"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOG41205.1"
                     /translation="MRIHKEGHTIIIINFLIWAAITGFCFVFCKNAVVNWIVAGVALV
                     MSLFMIWFFRVPVRDAVVDDSLVISPGDGKVVNVKEVEENEYFGEKCLCISIFLTFFN
                     VHVTWFPVGGVVKYYKYHPGKYIFAFHPKSSEKNERTTIAMRTDSGNELMFRQIAGIV
                     ARRVVCYAKENEYFGQNAEAGFIKFGSRLDVFLPLSTEIFVKKGDKVSGQTSVLAKF"
     gene            complement(4257..6107)
                     /locus_tag="PLI69_01540"
     CDS             complement(4257..6107)
                     /locus_tag="PLI69_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOG41206.1"
                     /translation="MKYLKSIIMSLLLVMLLASCNEWLNVNTDPDNPSSESALFQNRL
                     AHIEFYTNHAQQFAAWRTSMSMGDWTRYNGGGTYFNMSIWYPVGGICTTPYQWWFVGA
                     ACNLNDMYKRAEEAEAWHYMGAFKIIRAYGFMMMTDLYGEMPYTDALGENATPEYDDG
                     KTIYLGCLEELDEGLALMAKTQAPTTPALALGDYWGNGDTQKWIKWGNLLKARFINKL
                     IKKGAGSYKEGKFDSQAILDALAKGPQSINDNMVIYHTDNNSTTHDVLGWNEPVDYSP
                     LYSVSGMNAGYMPTKMLFDNLTNFAGYGVEDPRADRILPWAYDANPRAGAPADVKWSG
                     NWRRSRGVDMTSGDCPIFQGGPLRANFSTTNGWFIQSDNPARPNDIYYVEATSSSKGY
                     AANPDLLYRRAGKTVDGSRESGTFYTRVSSPTYMGSYAEACFIKAEVLFNKGDKSGAF
                     TAYKEGVKASMDQMNIKLNQWVAEDPGLLECPSFVPMTTAEINNFLNNGIGTAGDLSL
                     GKILTQKRIALHFSVEIWNDMRRYDFDTKLFFNWKIPAYHDVSAGALKAIPMGKHFRR
                     WMQCSHEYNYNKTNLQAIGEKVPGADMTKPMWNTVDDVWTIPVWWDSNQN"
     gene            complement(6139..>9272)
                     /locus_tag="PLI69_01545"
     CDS             complement(6139..>9272)
                     /locus_tag="PLI69_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOG41207.1"
                     /translation="INVTGTVTDKSGDPLVGVYVVVRGTQTGTATDIDGFYTISVPAN
                     GTLEFTSMGYKTATVAVAGRAKIDVVLEDDALLLEDAVVTALGIKKERKSLGYAVEDL
                     KSEELMKIKTANPISSLSGKIAGVNITQSSGAAGSGAQIILRGGTSASEGKDNQPLFV
                     VDGIIYDNSSTVVGNSAFDGSYRSATTTSNRVMDINPEDIESMSVLKGPAASALYGSR
                     AANGVVLITTKRGQEGVVEVNFSSKLSTSWAKALPKFQNQYTMGFMEDQYDSSKKYIG
                     TVFNDYSYNSWGKEGGVFYDNMGTFFDNGLILDESISISGGTKQGSYYLSGSLFDQNG
                     IVPTTGYSKYTFRFNGDQKIGIFTFSASAAYSKAHTDKTLTGAGLYGSAGTGALYSVY
                     TWAPSDNMSRYLNEDGTRFRHFADRQDPWDDRDNPYWIINKFNIFDDTDRFTGNLNVR
                     ADITPWWNISYKIGIDRYTQVDSKRIAAGSAIKQVFQKGMMSDNTRQYEYMTNSIISD
                     MNKKFGDFEVGLMLGAATDQYKTNSDYLMAYNFSVPDFFSYANTTTDNKAFSHGSSLK
                     RLIGVFGEARASWRNMVYLTVTGRNDWTSTLPLDSRSYFYPSVSGSFVFTQLLQDLNV
                     IDDSILSFGKVRVSWAKVGKDTGVYETATALWPVGTYLGELVGVGNSWTRGNPYLKPE
                     MTKSTEIGVELSFFKNRLHIDYAYYTNDSFNQILSPRGPQSTGYIFCSINAGNVYNKG
                     MELSISGTPIETRNFRWDVGLNMAGNRGTMDGLPSGTDVMYVTDVQYGPAKAASYNGG
                     DFMAIAGYKWSRNKAGNVILDKNGFPTYAANTYYAVGNREATFAGGFNNTIQWKGFTF
                     NMLWEFRVGGDVFNGTQYAMDVAGTSQFSADIRNKPLTINGDFQVGTVKNADGEDEPV
                     YEARSNTWDPKQVYDFNGVPTSGQNIIKNYYTNYYTQEAANYITKVNLLRLRALSVAY
                     EFPKKWMEKTGFIKRASLSLSANNLLLFTNYKGDPEVAVAGAGVGGSSSVGFAYCDVP
                     ATSGMTFGLNLTF"
BASE COUNT         2417 a         2021 c         2237 g         2597 t
ORIGIN      
        1 gcttgaaaag tattgaaaat gtgaggtttt cgcctctttt gctctcccag cgattgcctc
       61 tttgacctct ccccgccgtc tggaaatagg ctgcatagac agcgagattc tccagattgt
      121 ggcggtcatt ttgcaattgt gtattggtgg aatccgtctc ttcaaaccat ttaatgtgga
      181 gtgtgccgat cattaatctc tctataattt taggttggct aaaaatttgc agtatatttg
      241 tcgcaccaag ttagtaattt tcagatgaag aaaaccaaag aagttaataa aagtgagatt
      301 gagaccaatc tcaacgagat aaatgtcata gctgatgcta tgatggagaa aaagggccat
      361 gatgttattt ccctagattt aaggcgcatc aacacagcat ttacagacca ttttgtgatc
      421 tgtaatgcag atacgcccat ccaggtggtt gccatcagtg actatgtaga ggagatgatg
      481 gaaaaaaagg cagatagacc tgcagtaagg aaacaaggca gggaacatgg tttctggatt
      541 attctcgact acacagatat tgtcgtccat attttcaaga cagagcagag attgttctac
      601 cgactggaag atctttgggc tgatgctgtg aaaactgttt ttgaggaggt ttaatatggc
      661 tagtaacaca cgcaatacgc gcggcggcag accgaccggc aaacctaata gaagaaaagc
      721 acccaactgg ctcttttggt tttatattct actctttgta ggactgggtt atatgtttct
      781 tacaagcagc ggagttgaac ccattaaaaa ggaatggatt gaggtaaagg aagagattct
      841 tcccagcgga gatatagata aggtagttta tattcgcaat gagcgcaggg gtgatattta
      901 tatctctaag gatagtattt ccgagtacaa aaatattttc ggaggtaagg aacccaagtc
      961 aggtccccat ttttatttcc ttgtttccga caaatttgac gccgaagtag aactagaggc
     1021 tgcacgagag tcacttggag agggacgtaa atttgacgtg gtagtggagg agaaacacaa
     1081 ttactggggc agagccctgg aatggctgat gtttcctttg cttttaatcc tcatgtggtt
     1141 cctgcttttc cgtcctatgc gtagcaatat gggcggtggg gccggtggcg gcatatttaa
     1201 tgtcggtaaa tcccaggcaa aactctacga aaaggaaaac aacatcaaga ttacattcaa
     1261 agatgtagca ggtttgcaag aggcgaaagt tgaaatcatg gagattgtgg atttcctcaa
     1321 gaatcccaag aaatataccg ctttaggtgg aaaaattcct aaaggagtac ttcttgtcgg
     1381 ccctcctggt acaggaaaga ctcttcttgc caaagctgtg gcaggtgagg ccaacgttcc
     1441 cttcttctcg atttcaggct cagacttcgt cgaaatgttt gtaggcgtag gagcatcaag
     1501 ggtgagggat ctcttcaaac aggcaaagga aaaggctccc tgtatcgtat tcatagatga
     1561 aatcgatgcc gtaggtcgtg caaggagcaa aaacctgggt tactcttcca acgatgaacg
     1621 tgagaacact ctcaaccagc ttcttaccga gatggacggt tttggttcca attccggagt
     1681 cataatactt gctgccacaa acagaccgga tatcctcgac aaggcactca tgagagcagg
     1741 ccgttttgac cgacagatac acctggatct tcccgagctc aaagaacgtc tagagatttt
     1801 caaggtgcat cttcgcaacc ttaagcttgt cagtggcttt gatatagatt tcctagccaa
     1861 gcaaactccc ggattttcgg gtgccgatat tgccaacgtt tgtaacgaag cggctctgat
     1921 tgctgccaga aataacaagg agtttattga cagacaagat ttcctcgatg caattgacag
     1981 gatagtgggt ggtcttgagc gtaagagcaa attcatttcc ccggaggaaa agcgcactat
     2041 tgcgtaccac gaggcgggac atgctacagt aagttggctg ttgcccaatg ccaatcctct
     2101 gctaaaagtg accattatcc cccgaggaca gtcgcttggc gcagcctggt atctccccga
     2161 tgagcgccat ctcacgacaa aagaacaact gaaggatgaa atggcaagta ccattgcggg
     2221 tcgtgtggcg gaagagataa taaacggcaa aatctccacc ggtgcgctca gcgatctgga
     2281 aaaactcagc aaacaggctt atgcaatggt cacttttttt ggcatgagtg ataaggtggg
     2341 taatgtctct ttctacgact ccaccggacg ttcagagatg gcactttcca agccatatag
     2401 tgaaaagact gcggaactta tcgattctga agtaaagaga tttattgatg aagcgcataa
     2461 gacggcaacc cgcatactta acgaaaatcg tgaaggcttt gagcaactgg cagaactcct
     2521 tttggagaaa gaggttatat tctctgagga tcttgaaaag atattcggtc cccgtaaagg
     2581 gggaaaagat cccaacaagt tgctggcgta gcctatgagc tctttgtcac agcgcatcac
     2641 aagcggtatc atctttttta tagtagttgc gggagcactg ctatgcagtt gtaccttcct
     2701 ccctcttacg gtggcaatag gtgcgttgat gatgagtgag tactattcga tgactctggc
     2761 aaaacgtttt ttcgcagagc agatacttat gattttggct gcgactctag ccttcgtact
     2821 ttttttcctg acctttaaaa ttgatcttaa ccacggatac atatttctgg tgttcatacc
     2881 tgttcttgct gcctgcatct cttttatcta tacttcaaca agagatttgc gcagaggtga
     2941 ggcagatagg ggagtatctc gtgatattgt caatagtgtg gcgcatctga tgatgccggc
     3001 gctctatatt gtggccccgt tgctacttgc acagtttctg gtcttcggac ttaagggagt
     3061 gtacactccc tggctgctgc tcagcgttat gatattgatc tggctcaatg atgttggggc
     3121 ctatatgttc ggaatgttgc taggacagcg tcagggcagt cgtaagctct ttccaagcgt
     3181 ttcacctaag aagtcctggg ccgggttcct gggaggtgcg ctgataacct ttttgatttc
     3241 aatcctgctc tattatgtaa ccggcagcct atcgttattc tatatttcct ggtggcattg
     3301 gctggcgatg gccgccatag tctcagtttt cggcctcttc ggtgacctct tcgagtcttt
     3361 gatcaagaga gcctgcaatg tcaaggattc aggcaatata atgccgggcc acggaggtct
     3421 tctggatcgt tttgatgcaa ccttattcgt tatccctgca attgtgctct acctcaagct
     3481 tgtttgtgtt atttgaaggg attgcatatc tttgaatcca aattaatcca taaatgagaa
     3541 tacacaaaga aggccatacg atcattatca tcaacttcct gatttgggct gcaatcaccg
     3601 ggttctgttt tgtgttttgt aaaaatgcgg ttgtcaattg gatagtggcg ggtgtagcgc
     3661 tcgttatgtc gctcttcatg atttggtttt tcagagtccc cgttcgtgat gccgttgtcg
     3721 acgattctct ggttatatct cctggagatg gtaaggtggt caatgtcaaa gaggttgagg
     3781 aaaatgaata tttcggtgaa aaatgcctct gtatctcaat tttccttacc tttttcaatg
     3841 ttcatgttac ctggttcccc gtaggaggtg tcgtaaaata ttacaaatac caccccggca
     3901 aatatatatt tgctttccat cccaaatctt cggaaaagaa tgaacgcacc accattgcca
     3961 tgcgtaccga ttcgggtaat gagttgatgt ttcgccagat tgcgggtatc gtagccagac
     4021 gcgtagtctg ctatgcaaag gagaatgagt acttcggcca gaatgcagaa gcaggtttca
     4081 taaaattcgg ttcaagactg gatgtcttcc tacctttgag tacggagatt tttgtcaaaa
     4141 agggagataa ggtttccggc cagacttcag ttctggcaaa gttttaagtt actacccact
     4201 acttactaca gactacatac taaaaaaaaa tccacccgaa ggtggatttt tttaaattag
     4261 ttctggtttg aatcccacca tacgggaatg gtccaaacat cgtctaccgt gttccacata
     4321 ggtttggtca tatctgcacc gggaaccttc tctccaattg cctggaggtt ggtcttgttg
     4381 tagttatact catgtgaaca ctgcatccag cgacggaaat gcttgcccat gggaatagcc
     4441 ttgagagcac cggcgcttac atcatggtaa gcaggaatct tccaattgaa gaagagtttt
     4501 gtgtcaaagt catagcggcg catatcattc cagatttcaa ccgagaagtg taatgcaata
     4561 cgtttctggg ttagaatctt accaagtgaa aggtcacctg cagtaccgat accgttattg
     4621 aggaaattgt taatctcggc agtagtcata ggtacgaatg aaggacattc aagcagaccg
     4681 ggatcttctg caacccactg gttcagtttg atattcatct gatccataga tgccttaaca
     4741 ccctctttgt aagcagtgaa tgctccgctc ttgtctccct tgttgaagag cacctcagct
     4801 ttgataaagc aggcctctgc ataggatccc atataggttg gagatgatac tctggtgtag
     4861 aaagtacctg attcacgaga accgtcaaca gtctttcctg cccttcgata gagaaggtcg
     4921 gggttggctg catagccctt cgatgaagaa gttgcctcta cataatagat atcgtttgga
     4981 cgagcggggt tgtcactctg gataaaccat ccgttagtag tgctgaagtt ggcacgtaga
     5041 gggccaccct ggaagatagg gcagtcgcct gaggtcatgt caacaccacg tgaacggcgc
     5101 cagttgcctg accatttaac atcagcagga gcacctgctc tggggtttgc atcataagcc
     5161 cagggtagaa tcctgtctgc acgagggtct tctacaccgt agccggcaaa attggtgaga
     5221 ttgtcaaaca acatcttggt aggcatataa ccggcgttca taccactgac agagtagaga
     5281 ggtgagtagt ccaccggctc attccatccg agtacgtcgt gagttgtgct attattatcg
     5341 gtgtgataga ttaccatatt gtcatttatg ctctggggac ccttagcaag tgcgtcgagg
     5401 atagcctgag aatcaaattt gccctctttg tagctccctg cacccttctt tatgagtttg
     5461 tttatgaagc gtgctttgag cagattaccc catttgatcc atttctgagt atcaccgtta
     5521 ccccagtagt cgccaagtgc gagagcagga gtagtggggg cttgagtttt ggccataagt
     5581 gccagacctt catcgagctc ttccagacat ccgagataga tggttttgcc gtcatcatat
     5641 tcgggtgtgg cattctcacc taacgcatcc gtatatggca tttcaccgta gaggtcggtc
     5701 atcatcataa aaccgtatgc acgaataatc ttgaaagcac ccatgtagtg ccatgcttca
     5761 gcctcttcag ctcttttgta catgtcgttg aggttgcagg ctgcacctac aaaccaccat
     5821 tgatagggag tagtgcagat acctcctaca ggataccaga tagacatgtt gaagtaggta
     5881 ccaccgccat tgtatcttgt ccagtcaccc attgacatgc ttgtacgcca tgcagcaaat
     5941 tgctgagcat gattggtata gaactcaata tgcgcaagac gattctggaa caaagcactc
     6001 tctgatgagg gattatcagg gtcggtgttc acattaagcc actcattgca ggatgcgagg
     6061 agcatcacta agaggagtga cattattatt gatttcaaat atttcataat cttactgtta
     6121 tttatcattg tccatttgtt agaatgtcaa gtttagaccg aatgtcatac ctgatgtagc
     6181 aggaacatca cagtaggcaa aacctactga ggaggaacca cctacacctg caccggctac
     6241 agcaacctca ggatctccct tgtagttggt aaagagaagg aggttgtttg ctgagagtga
     6301 gagggaagca cgtttgataa agcctgtttt ttccatccat ttcttgggaa actcgtatgc
     6361 tacagagagc gcacgaagtc tcaggaggtt aactttagta atatagttag ctgcctcttg
     6421 agtgtagtag ttggtatagt agttcttaat gatgttctga ccacttgtag gaacaccatt
     6481 gaagtcatat acttgtttag gatcccaggt gttgctcctg gcttcataaa caggttcatc
     6541 ttctccatct gcattcttga cagttccaac ctggaagtcg ccattaattg tcagaggttt
     6601 gttcctgata tcggctgaga attggctggt accggccaca tccattgcgt attgtgtacc
     6661 gttgaatacg tcaccgccta cgcggaattc ccaaagcatg ttgaatgtga aacccttcca
     6721 ttggatggtg ttgttaaaac ctccggcgaa ggttgcttca cggttaccaa ctgcataata
     6781 ggtgtttgct gcataagtgg ggaatccgtt cttgtcaaga attacattgc ctgccttatt
     6841 gcgtgaccat ttgtaacctg caatggccat gaagtctcct ccgttatagg atgctgcttt
     6901 cgcgggacca tactgcacgt cagttacata cattacatca gtacctgagg gcagaccatc
     6961 cattgtaccg cggttaccgg ccatattcaa tcccacatcc catctgaagt ttcttgtctc
     7021 tatcggggta cctgagattg agagctccat acccttgtta taaacgttac ctgcgttgat
     7081 agagcagaat atataacctg tggactgagg tccgcgaggg cttagaatct ggttgaatga
     7141 gtcgttggtg tagtatgcgt agtcaatgtg aagtctgttt ttgaagaagc tcaattcaac
     7201 tccaatttcg gttgattttg tcatctccgg tttcaaataa gggttaccgc gggtccagga
     7261 gttacctaca cccactaatt cgccaagata agtacctaca ggccacaaag cggtagctgt
     7321 ctcataaacg cctgtatcct tacctacctt tgcccatgaa acgcgaacct ttccgaatga
     7381 taagatgctg tcgtcaatca cattcaaatc ttggagaagt tgtgtaaaga caaatgatcc
     7441 gcttactgaa ggatagaagt aagagcggct atccagagga agcgtggatg tccagtcgtt
     7501 acgtccggta acggtcagat aaaccatatt tctccaagat gcacgtgcct caccgaatac
     7561 acctatgagt ctcttgagag atgaaccgtg tgagaaggct ttgttgtcag tggtggtatt
     7621 ggcatatgag aagaagtcgg gaaccgagaa gttataagcc atcagatagt ctgaattggt
     7681 cttgtactgg tcggtagctg cgccgagcat caaacctact tcaaaatctc caaatttctt
     7741 gttcatgtcg gaaattatac tgttggtcat atactcatat tgcctggtat tatctgacat
     7801 catacctttc tggaatactt gcttgattgc agagcctgca gcgatgcgtt tggagtctac
     7861 ctgagtataa cggtcgatac cgatcttgta ggagatgttc caccatggag tgatatcagc
     7921 tcttacattg aggttgcctg tgaaacggtc tgtatcgtca aaaatgttga acttgttgat
     7981 aatccagtag gggttatcac ggtcgtccca aggatcctga cgatcagcaa agtgacggaa
     8041 acgagtgcca tcttcattga gatatctgct catattgtcg gagggagccc aggtataaac
     8101 agaatagaga gcacctgtac ctgccgagcc atagagacct gcaccggtaa gagttttgtc
     8161 cgtatgagcc tttgagtaag ctgcacttgc actgaatgtg aagataccta tcttctggtc
     8221 accattgaaa cggaaagtgt acttactgta gcccgttgtg ggtacgatac cgttctggtc
     8281 aaatagagaa ccggagaggt agtaactgcc ctgtttggta cctccggaaa tgctaatgct
     8341 ctcgtcgagg atcagaccat tgtcaaagaa tgtgcccatg ttgtcataga agacaccgcc
     8401 ttccttgccc catgagttgt aggagtagtc gttgaaaaca gtaccgatgt atttcttgga
     8461 agaatcatac tggtcctcca tgaatcccat ggtatactgg ttttggaact tgggaagtgc
     8521 ctttgcccag gaagtagaaa gttttgaact gaagttaacc tcaacgacac cttcttgacc
     8581 gcgtttggtg gtgataagca cgacaccgtt agcagcacgc gaaccgtaaa gtgcggaagc
     8641 ggcagggcct ttgaggactg acatcgactc gatatcctca gggttgatgt ccattacacg
     8701 gttggaggtg gtagtggcgc tcctgtagga accgtcgaat gccgagttgc cgacaactgt
     8761 agatgagttg tcatagatga taccgtccac aacgaagaga ggctggttgt ccttgccctc
     8821 tgatgcggaa gtaccaccac gtagaataat ttgtgcgcct gaaccggcgg cacctgaaga
     8881 ctgggtgatg ttgacaccgg cgatctttcc ggagagggag gagatcgggt tggcagtctt
     8941 aatcttcatc agctcttcag acttgagatc ttccactgcg tatccgagag attttctctc
     9001 cttcttgata cccaatgccg tgaccacagc gtcctcgagc aataatgcgt cgtcttctag
     9061 taccacatca atctttgctc ttccggctac tgctacagta gcggttttgt agcccattga
     9121 tgtgaattcc aaggtgccat ttgcaggaac cgaaattgtg tagaaaccat caatgtcagt
     9181 ggccgtaccg gtttgagtac cacgcactac cacatagaca ccgacgagcg gatcaccgct
     9241 cttgtcagtc accgtaccgg tgacattgat gt
//
LOCUS       DAOSIE010000024         9183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_7174_length_9183_cov_4.088848, whole genome shotgun sequence.
ACCESSION   DAOSIE010000024 DAOSIE010000000
VERSION     DAOSIE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9183
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7174_length_9183_cov_4.088848"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLI69_01550"
     CDS             <1..257
                     /locus_tag="PLI69_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOG41208.1"
                     /translation="RRALKILPSELHWIRPDLALKIGEELECDIRIRYRQPLQKGRLI
                     CRDEALYILFNEPQRGITPGQFAVWYLPDGEMFGSGVIHD"
     gene            294..1727
                     /locus_tag="PLI69_01555"
     CDS             294..1727
                     /locus_tag="PLI69_01555"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG41209.1"
                     /translation="MTSFRKLFLLSLVTAFTLVSCGDPANPTPVLSDAANLNSVSFST
                     VDNPSMSVSAHSTPCLGYLAVTVPEGSNISALKATYYVSDGAEVLIKGQKQVSGQTIN
                     DFSNIVDLQVVSESGKVKKDYVLYVKSGNASVDARFYQFMNKHSVPGVSFSVMRGEQV
                     VYTTGYGYAVTESRTKTTPDHLFRLASISKQFTTLCIMVLYERGMLGIDDKVFGENGI
                     LKKEFKTKGMEDMITVRHLLQHSAGWTREVLDPMFSSVRNGKTLDELLQWVLDNQNLK
                     FTPGSNYEYSNVGFSFLGRIVEVVSGKPFETFLKEDVLKPMGVTDIHVGGGQDERRPN
                     ECVYYSQDGTNGYGNPMQVIAACGGIIASTNQMMTVLAHIDGRDGVPDILKPETLDLM
                     YNSEIDPNADPSTNLRRYGLGWRRLAGYYPGAHWHGGNIAGTATIWVGNTNAGMSAAI
                     LCNSRSYDKSIDGDMQTMIRDLMEVFQ"
     gene            1737..3035
                     /locus_tag="PLI69_01560"
     CDS             1737..3035
                     /locus_tag="PLI69_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG41210.1"
                     /translation="MKYGGEGGDMSDIIVIGGGPAGMMAAIKAAERGTKVTLLEKNNI
                     CGKKIFITGKGRCNLTNTKSWSEFSTHIHPSANFLKPAFFNFSNAATIDYFNSIGLPT
                     TVEQGERVFPRSMRAADVPAVLVERMEKLGVEVICSSHVLNVSYSDALFHCLCSRSDS
                     LRGERMDMVKYSAPVLIIATGGLSYPGTGSTGDGYEFAKSLDHSVTETFPSLTALMPR
                     KYDTDLVGIELKNVGLSLFIDMDLIKIEDGDLTFTSNGIEGALGFRLSRRAVLALMNG
                     QRVELVLDLKPALSLEKLRARISRDLERMGVTAANVGRNRFRTLLRGLMPAALVDPFQ
                     HHHPDLSLSNLPDKLKEWRFEIKSYTGYERAVVTAGGIPQKEIIAKTMASKKQSNLYF
                     AGEILDIDGDTGGYNLQVAFSTGALAGQSAAQQILKLKSL"
     gene            complement(3005..3517)
                     /locus_tag="PLI69_01565"
     CDS             complement(3005..3517)
                     /locus_tag="PLI69_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41211.1"
                     /translation="MNLIVFPYDCDNYYFRPDTTMVHEARDFYCPDIITVLEAAPCIC
                     IRICKSGKAISERFAGRYYDAVGFGVTLYAGNILAEGGLFALTQSTSFDATTVIPIPL
                     KPAESLQELCPEITQEYIGRKMAVISRNTLLRIGDMLIFELAPRSIVDKSLPFTLEWR
                     GQRLFSFNIC"
     gene            complement(3514..4308)
                     /locus_tag="PLI69_01570"
     CDS             complement(3514..4308)
                     /locus_tag="PLI69_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG41212.1"
                     /translation="MVLDLKRPILVFDIETTGLSVAEDRIVEISVIKLFPDGSREHKT
                     RRINPEIPVPEESRMIHGISDEDVKDCPTFKQIAKSLADWMSGSDIAGFNSLKFDIPI
                     LAEEFLRAGVEFDFRQIEMIDVQNIFHKMEPRTLVAAYRFYCNKELDNAHSAEGDAIA
                     TLEVLEAQVERYHELQNNVKFLASFSERGKMVDFAGRIARDSSDEPIFNFGKHKGKRV
                     VDVFKSEPSYYDWMMKGEFTRDTKQVITDIWMKHKEGELKDLFNSR"
     gene            complement(4313..5431)
                     /gene="dnaN"
                     /locus_tag="PLI69_01575"
     CDS             complement(4313..5431)
                     /gene="dnaN"
                     /locus_tag="PLI69_01575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOG41213.1"
                     /translation="MRFSVSSSELLHGLVSVSRVIANKPSSSILENFLFDLKDNALTV
                     TASDGETTLKTVISIENVQQEGAFAVPARRLTDYLKEFPDQPLTFYGHDNTMEITWIN
                     GESKIPCFNADDYPQLPALGETHDTLEIPAETLLNGINNTIYATAEEELRPVMNGIFF
                     DLTPESSAMVASDAHKLICFEFKDANVAQQCSFILHKKPAAILRSILPKVEEKISIVF
                     DSKNAYFNFGTNMLVCRLIEGKYPQYRSVIPKNDNKLTIGRTDFLNSVRRIAVCSAQA
                     SNQIKLKLSLNELVVSAQDLSFSTSAYERLSCNYEGDEMEIGFKAQFLIEILTNLPYT
                     NVCMELADPFKAVLIVSADETDPDEELRALLMPVMINV"
     gene            5554..6684
                     /locus_tag="PLI69_01580"
     CDS             5554..6684
                     /locus_tag="PLI69_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOG41214.1"
                     /translation="MKRNTLFLLITLLLSLTISWLVADKVVETKLAAAAAEADGKRSS
                     IFTSAPGSANRTVISTNMGDFSDAAETAVHTVVYVKVVKRAAKMPSSIMDLIFGFATP
                     KDQVGMGSGVIIREDGYIVTNNHVIAGADQIEVTLENNKSYPARLVGTDPATDLALLK
                     IDEDSLPSIVLGDSDNLRLGEWVLAIGSPYDLRSTITAGIVSAKGRSLPNYDGQYRVE
                     SFIQTDAAVNPGNSGGALINTMGELVGINTSIISLTGSYAGYSFAVPVNIVKKITDDL
                     IRYGEVRRAMLGISMTDITEGMARQFSLESMEGVYVAEVQPNSAADKGGIRVGDIITE
                     INLNKVKNGAAVQEQMNRLRPGEQAAITIQRGGKQIELIVSL"
     gene            6813..7673
                     /locus_tag="PLI69_01585"
     CDS             6813..7673
                     /locus_tag="PLI69_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOG41215.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGREELITVEEEVELAQRIR
                     KGDPEALEKLTKANLRFVVSVAKQYQNQGISLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKALSKFEQEHERMPSPDELSE
                     ILDIPREKIADTLRVSGRHVSVDAPFVDGEDNSLLDVLVNNDSPNADRGLVNESLNKE
                     IERALSTLTERERDIVKYFFGIGCQEMTLEEIGEHFGLTRERVRQIKEKAIRRLRHSA
                     RSKLLKSYLG"
     gene            7706..>9183
                     /locus_tag="PLI69_01590"
     CDS             7706..>9183
                     /locus_tag="PLI69_01590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOG41216.1"
                     /translation="MKKLILPLLAVVMMTACTETNPLLGDWKAPFEIPPFEQVLPEHY
                     LPAYIKAMEVHSAEIDAITSNPEPATFENTIAAYSYSGELLSRVSAVFGSASGVKSND
                     EIKAIARELSPLTSAHYDNILLNEALFARIKEVYDKRDSLGLEPDQMRLLTETYKGFA
                     RSGANLPPDKKEELKKINTEIAANQLAFGQNLLKETEWTLLVEDEKDLSGLSEAQIAT
                     AAARAQQAGKEGWIFGLDNPSVMPFLQSADNRELRIKMLDAYLNRCNNGNEYDNNEVI
                     KNLIRLRLQKANLLGYEDFAAYVLEDRMAKNADAVYALLNQLWTPAIAVAKAELADIE
                     ALAAKDGIKEVVAADWRYYFEKSMQSKYNISDEELRPYFKLENVRDGIFLLANKLFGI
                     TFEQLDNVPLPHAEAVAYECKEADGTTLGILFMDMFSRPGEKRGGAWCGGFRSQYYED
                     GKRVLPLVTIVGNFTRPSGDKPALLSTDETNTFFHEFGHALAS"
BASE COUNT         2500 a         2040 c         2191 g         2452 t
ORIGIN      
        1 accgccgtgc actgaaaatt cttccttcag agctccactg gatcaggccc gacctcgctt
       61 taaaaatagg agaggagtta gagtgtgata tccgtatccg ataccgacag cccctgcaga
      121 aaggtcgcct tatatgtcgc gatgaggccc tctatatact atttaatgag ccccagagag
      181 gtataacacc gggccaattt gcggtctggt atttaccgga cggggaaatg ttcggaagtg
      241 gcgtgatcca tgactgattc ttcatacaag tttaacctca aatcaaaatc ataatgactt
      301 cttttagaaa actatttctg ctctcgctgg taacagcatt tactctggtt tcctgtggtg
      361 atccggccaa tccgacaccg gtcctttcgg atgctgcaaa cctaaattcc gtctccttct
      421 ctacggtaga caatccttca atgtccgttt cagcgcattc tactccctgt ctgggttatc
      481 tggcggtgac agtccccgaa ggttccaata ttagtgcact caaggcaact tattatgtct
      541 ctgatggtgc ggaagtgttg ataaaaggac agaagcaggt gagtggtcaa accatcaatg
      601 acttctcaaa tattgtagat ttgcaggtgg tatccgagtc cggaaaagta aagaaagatt
      661 atgtcctcta tgtaaagagc ggcaatgcat cggtagatgc gaggttctat cagtttatga
      721 ataaacactc agttccgggg gtctcttttt cagttatgag aggcgagcag gtagtctata
      781 ctacaggata cggctatgcg gtaacggagt cgaggacaaa aaccactccc gaccatcttt
      841 tccgcttggc gagcatatcc aaacagttta cgacactctg cataatggtg ctttacgaga
      901 ggggaatgct tggaatagat gataaggttt ttggtgaaaa tggtattctc aaaaaagaat
      961 tcaaaaccaa aggtatggag gatatgatta ccgtacgcca cctcctccaa cactcagccg
     1021 gatggacaag agaggtactt gatcctatgt tcagcagtgt cagaaacgga aaaactctcg
     1081 acgaactcct tcagtgggtg ctcgacaatc agaatcttaa atttactccc gggtccaact
     1141 atgagtattc caacgtagga ttctcattcc tgggccgcat tgtagaggtt gtgagcggca
     1201 agccttttga aaccttcctt aaggaagatg ttctaaagcc gatgggtgtt actgatattc
     1261 atgtgggagg tggccaggat gagcgcagac ctaatgagtg tgtctattat tcacaggacg
     1321 gcaccaatgg ttatggcaac cccatgcagg tgatcgctgc ctgtggcggt atcatagcat
     1381 ctaccaatca gatgatgacg gttttggcac acattgacgg tagagatggt gttccagata
     1441 ttctgaagcc ggaaactctc gatctgatgt ataattccga aatagatccc aatgcagatc
     1501 cgagtaccaa tcttagaaga tatggtcttg gatggagacg tcttgccgga tactatcccg
     1561 gtgctcactg gcacggtggc aatattgccg gtactgctac tatatgggtt ggcaatacaa
     1621 atgccggaat gagtgcggcc attctttgca actcccggag ttacgacaaa agtattgatg
     1681 gtgatatgca gaccatgatc cgcgacctca tggaggtatt tcagtaatat taataaatga
     1741 aatatggtgg ggaaggaggt gatatgagtg acattatagt gataggagga gggccggccg
     1801 gtatgatggc tgccatcaag gcggcagaaa gaggtactaa agtcaccctt ctcgagaaaa
     1861 ataatatttg cggtaaaaag attttcatta ccggaaaggg aaggtgcaat ctgaccaaca
     1921 caaagtcttg gtcggagttc tccactcata tccacccctc ggcaaatttt ctaaaaccgg
     1981 catttttcaa tttcagcaat gctgcgacta tagattattt taactcaata ggtttgccca
     2041 ccaccgtaga gcagggtgag cgggttttcc ctcgttcaat gcgtgcggca gatgtgccgg
     2101 cagtgcttgt agagcggatg gagaaattgg gagttgaagt aatatgctcc tctcatgtgc
     2161 taaatgtctc ttatagtgat gctcttttcc attgtctctg cagcagaagt gattctttgc
     2221 gtggagagcg tatggatatg gtgaaatact ctgctccggt actgataatt gcgaccggag
     2281 gtctttctta tcccggtacc ggctctaccg gtgacggata tgaatttgca aagagcctgg
     2341 atcactcggt tacggagact ttcccctcac ttacggcttt aatgccacgg aaatacgaca
     2401 ctgatttggt tgggatagag ctgaaaaatg taggactttc tctctttatc gatatggatt
     2461 tgataaaaat agaggatggc gaccttacct ttacctccaa tggcatagag ggagcactcg
     2521 gtttcagatt gagcaggcga gcagtattgg cccttatgaa tgggcagaga gttgaacttg
     2581 tgctggacct caagccggcc ctttcgttag aaaaactgcg ggcgcgcatc tccagagatt
     2641 tggagcggat gggagtgacg gctgccaatg tgggacgcaa cagatttcgt actcttctac
     2701 gcggacttat gccggcagca ctcgtagacc ctttccagca tcatcacccg gatctaagcc
     2761 tttccaacct tccggataag ttaaaagagt ggagattcga gattaaatct tataccggat
     2821 atgagcgcgc agtagtcacc gccggaggta ttcctcagaa agaaataatc gccaagacta
     2881 tggcttccaa aaaacaatcg aatctctact tcgcgggaga aatactcgat attgacggag
     2941 atacgggagg ttacaatctg caggtggcct tttctaccgg agcccttgcc ggtcaatccg
     3001 ccgctcagca gatattgaag ctgaaaagcc tctgacctct ccactccaga gtgaaaggga
     3061 ggctcttatc cacaatactc ctgggtgcaa gttcgaagat cagcatatct ccaattctta
     3121 gcaaggtgtt gcgagatata actgccatct ttcggccgat atattcctga gtgatctccg
     3181 gacagagttc ttgcagactt tcagcaggtt ttagaggtat tgggatgacg gtagtagcat
     3241 caaatgacgt gctttgggtt aaggcaaaaa gccctccctc ggccagaata ttgccggcat
     3301 aaagagtgac tccaaagccg accgcatcat aataacgccc ggcaaatctc tcggaaatag
     3361 ctttgcctga tttgcatatc cttatgcaga tgcatggagc cgcttccaga acagtgatga
     3421 tatcgggaca ataaaaatcc ctcgcttcat gcaccattgt ggtatcaggg cgaaagtaat
     3481 aattgtcgca atcgtaagga aaaactatga gattcatctt gaattgaaga gatccttgag
     3541 ttcaccctcc ttgtgtttca tccagatgtc ggtaatcact tgctttgtat cgcgggtaaa
     3601 ttcgcctttc atcatccagt cataataact gggttcactt ttgaatacgt cgactactct
     3661 tttacctttg tgcttaccga aattgaatat cggctcatcg gaagagtccc gtgcaattct
     3721 ccctgcaaaa tctaccattt tacccctttc ggagaacgag gcgagaaact ttacgttatt
     3781 ttggagttca tgatatcttt cgacctgagc ctcaagtact tcgagggtag caatggcatc
     3841 tccctcggcg gagtgcgcat tatcaagttc tttgttacag tagaagcgat aagcggcaac
     3901 cagggttcgt ggctccatct tgtggaaaat gttttgcaca tctatcatct cgatctgcct
     3961 gaagtcaaac tcgacacctg ctcttagaaa ttcctcagcc agtattggaa tatcaaattt
     4021 gagggaatta aagccggcta tgtcggagcc gctcatccag tcggccaacg attttgctat
     4081 ttgcttgaac gtggggcaat ccttgacatc ttcgtcactg ataccatgaa tcatgcgaga
     4141 ctcttccggt acgggtatct cagggttgat tctgcgggtc ttatgttcgc gggaaccgtc
     4201 gggaaagagt tttatgacgc tgatttctac aatcctgtct tcagcaacgc taagtccggt
     4261 ggtctcaatg tcaaatacta ggatgggcct cttaagatcc agtaccatag ttctaaacgt
     4321 tgatcatcac aggcatcagc agtgcacgga gctcctcatc gggatctgtc tcatcagccg
     4381 acactatcag tacagcctta aatggatctg caagctccat acatacgttg gtgtagggga
     4441 gattggtaag gatttcaatc aggaactgtg ccttaaatcc tatttccatc tcgtcacctt
     4501 cgtaattgca ggaaagacgc tcataagctg atgtggaaaa actcaggtct tgtgccgaca
     4561 caactaattc gttgagcgat agtttgagct taatctggtt agaagcctgt gcggagcata
     4621 ccgcaatgcg tcttacggag tttagaaaat ctgtcctgcc aattgtgagc ttattgtcat
     4681 tcttgggaat tacactgcga tattgggggt atttgccttc gatcagacgg cataccagca
     4741 tgttggtgcc gaagttgaaa taggcatttt tggagtcaaa aacgatggaa atcttctctt
     4801 ctactttcgg caggatgctt cttaggattg ctgccggttt tttgtgcaga atgaaagagc
     4861 actgctgtgc aacattcgca tccttaaact caaagcagat gagtttatgc gcgtccgatg
     4921 ccaccatggc tgagctttcc ggggtaaggt caaagaagat accgttcata acagggcgca
     4981 gttcttcctc tgcagtagcg tatattgtgt tattgatacc attcaggaga gtttccgcag
     5041 ggatttccag agtgtcgtgt gtctcaccca gggcggggag ttgagggtag tcatctgcat
     5101 taaaacaggg tatcttggac tcaccgttaa tccaggtaat ctccattgta ttgtcgtgtc
     5161 catagaatgt aagcggctgg tcagggaact ccttcagata gtcggtcaat cttcttgcgg
     5221 ggaccgcaaa tgctccctcc tgttgtacat tctctatgct tatgaccgtc ttaagggtgg
     5281 tttcaccatc ggaagcggtc acggtaagag cattgtcttt taagtcaaaa agaaaattct
     5341 ccagaatgct ggaagaaggt ttgtttgcga ttacccttga tacagatacc aggccgtgca
     5401 aaagttctga acttgatact gaaaatctca tatttcgtaa ttttatatat atttatctct
     5461 aactgccaaa tctagtttgt gacccacaaa tgtaataatt ttttggcata tattttggtt
     5521 tattttatat atcctgcgaa taaattagaa cttatgaaac gaaataccct ttttttactg
     5581 attaccctac ttctttcact tactatttcc tggcttgtgg cggacaaggt cgtggaaaca
     5641 aagcttgctg cagccgctgc agaggctgat ggcaagcgat catccatttt tacgtcggcc
     5701 cccggatctg caaaccggac agttatttct accaatatgg gggacttttc ggatgctgcc
     5761 gaaacggcag tccatactgt tgtttatgtc aaagttgtca aaagggcagc gaagatgccc
     5821 tcttccataa tggatctgat attcggcttt gccaccccaa aagatcaggt gggcatgggt
     5881 tcgggagtca ttatccgtga ggatggttat atagtgacca acaaccatgt aatagccggt
     5941 gcggatcaga tagaggtgac tcttgaaaac aacaagtcct atcccgcgag gctggtcgga
     6001 actgaccctg ccaccgatct ggccctgtta aagattgatg aggactctct tccctccatt
     6061 gtccttggag actcggataa tttgaggctt ggggagtggg tgcttgcaat tggcagccca
     6121 tacgatttac gctctaccat tacagccggt attgtcagtg ccaaaggtcg ttctcttccc
     6181 aattatgacg gccagtatcg tgtagagtca tttattcaga cagatgctgc ggtaaatccc
     6241 ggcaatagcg gcggagcact tatcaatacc atgggggaac ttgtaggtat aaatacttcc
     6301 ataatttctc ttaccggttc ttatgccgga tactcgtttg cagtacctgt caatattgtg
     6361 aaaaagatta ccgatgactt gataaggtat ggagaggtac gtcgtgcaat gctcggcatc
     6421 tccatgacag atatcacgga aggtatggca cgccagttta gtctcgagag tatggagggg
     6481 gtctatgtag cagaggtgca gccaaacagt gctgccgaca agggaggcat cagagtcggt
     6541 gacatcataa ctgagataaa tcttaataaa gttaaaaacg gggctgcggt acaggaacag
     6601 atgaatcgtc tgcgtccagg tgaacaggca gcgattacca tacaaagagg tgggaaacag
     6661 attgaattga tcgtttcttt gtaaaagttg catttatttt gtaacttttt ccgaatatat
     6721 tcgtacaata tacacgtgcg ggaattgaat tgccgcatgt gtatcttttt attccaaact
     6781 attattacta aaacctttat tataatttcc gaatgagaca actcaagatt accaaatcaa
     6841 taaccaaccg cgaaagtgct tcgctcgata aatacctgca ggagataggc agggaagagc
     6901 tcattaccgt cgaggaagag gtggaactag cccaaagaat cagaaaaggc gacccggaag
     6961 cacttgaaaa gcttaccaaa gccaatttaa ggtttgtcgt ttcggtagcc aaacagtatc
     7021 aaaatcaagg aatcagtctt cccgacctca ttaatgaggg caaccttggt ttgataaagg
     7081 ctgcggaaaa gttcgatgag actcgcggct ttaaattcat ttcatacgca gtatggtgga
     7141 taagacaatc catcctgcaa gcgctggcgg agcagtccag gattgtacgc ttgccactca
     7201 accaggtggg ttcgctcaac aagatcaata aagctctctc caaattcgaa caggaacatg
     7261 agcgtatgcc ctcgcccgac gaactttccg aaattctcga tattcccagg gagaagattg
     7321 ccgatactct cagggtatcg ggtcgccacg tctctgtgga tgcccctttt gtcgatggtg
     7381 aggacaacag tctgctggac gtactcgtta ataacgactc ccccaatgcc gatcgcggcc
     7441 ttgttaatga atctctcaac aaagagattg aaagagccct ctctacactt actgagaggg
     7501 agcgtgatat tgtcaaatac ttcttcggca taggatgcca ggagatgact ttggaggaaa
     7561 taggcgagca cttcggactt acacgtgaaa gagtgcgcca gataaaagaa aaagctataa
     7621 ggaggctccg ccatagtgca agaagcaaac tcctgaagag ctacctcgga taacagattc
     7681 aaaaaagcaa aataatatca atacaatgaa aaaacttata ttaccccttt tagcagtagt
     7741 tatgatgact gcctgtacag aaaccaatcc gctacttgga gattggaagg ctccctttga
     7801 gatacctcct tttgagcagg tactacccga acactatttg ccggcatata tcaaggcaat
     7861 ggaggtacac tccgcagaga tcgatgcaat cacatccaat cccgagcctg caacctttga
     7921 aaataccata gctgcatata gttattccgg cgaactgctc agcagagtct cagcagtatt
     7981 tggtagtgct tcaggcgtca aatccaatga tgagattaag gcgattgcaa gagaactttc
     8041 ccctctgacg agtgcgcatt acgataatat ccttctcaac gaggcgctat ttgcacgtat
     8101 aaaagaggta tatgacaaga gagacagcct cggtctggag cctgatcaga tgcgtcttct
     8161 gactgagaca tataaaggct ttgcccgcag cggagccaat ttacccccgg ataaaaagga
     8221 agaactcaaa aagattaata ccgaaatagc agccaatcag ctcgctttcg gtcaaaatct
     8281 tcttaaagag acggaatgga cacttcttgt cgaagatgaa aaagacctct caggactttc
     8341 cgaggctcaa attgccactg ctgctgcccg tgcacaacaa gcaggcaaag agggttggat
     8401 cttcgggctt gataacccca gtgtcatgcc cttcctgcaa tctgccgaca atcgtgaact
     8461 gagaatcaaa atgctggatg catacctcaa tcgctgcaac aacgggaacg agtatgacaa
     8521 caatgaggtt attaaaaacc ttatcagact gcgtctgcaa aaggcaaatc ttctcggata
     8581 tgaggatttt gccgcttatg ttctggaaga taggatggcc aagaatgccg atgccgtata
     8641 tgctctttta aaccaactct ggactccggc aatcgctgtg gctaaagcgg agcttgcaga
     8701 tattgaggct cttgcagcaa aagatggaat taaagaggta gtagcggccg actggagata
     8761 ctattttgaa aaatccatgc agagcaaata caatatttct gatgaggagt tacgccccta
     8821 tttcaaactc gaaaatgtac gtgacggtat tttcctttta gcaaataaac ttttcggcat
     8881 cactttcgag caacttgata atgtacctct acctcatgcc gaggcggtag catacgagtg
     8941 caaagaggct gacggcacca ctttgggtat tttattcatg gatatgttct cccgtccggg
     9001 cgagaaacgt ggtggtgcct ggtgtggcgg cttccgctcg caatactacg aggatggtaa
     9061 gagagtgctt ccccttgtga ctatagtagg caattttaca cgcccttcag gtgacaaacc
     9121 ggctcttctg agtaccgatg agaccaatac ctttttccat gagttcggcc acgctctggc
     9181 atc
//
LOCUS       DAOSIE010000025         9010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_7322_length_9010_cov_4.378559, whole genome shotgun sequence.
ACCESSION   DAOSIE010000025 DAOSIE010000000
VERSION     DAOSIE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7322_length_9010_cov_4.378559"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..343
                     /gene="rplW"
                     /locus_tag="PLI69_01595"
     CDS             53..343
                     /gene="rplW"
                     /locus_tag="PLI69_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOG41217.1"
                     /translation="MGILIKPVVTEKMTIQGDKLNRYAFIVDRDANKVEIKKAVEEMY
                     GVSVIDVNTVNYHGKRKSRYTTAGMLTGRTNHYKKAFVTLAGDDKIDFYSNI"
     gene            348..1181
                     /gene="rplB"
                     /locus_tag="PLI69_01600"
     CDS             348..1181
                     /gene="rplB"
                     /locus_tag="PLI69_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOG41218.1"
                     /translation="MAVRKFKPVTPGQRNKVISAFDDITCTTPEKSLLKPIKRTGGRN
                     NQGKMTMRYIGGGHKRMYRVIDFHRDKDQYTATVKTIEYDPNRSARIALVVYGDGEKR
                     YIIAPNGIKVGQVISSGPGVAPEIGNALPLSEIPLGTLVHNIELRPGHGAAMARSAGT
                     FAQLTAREGDHAILRLPSGETRMVLVTCRATVGTVSNPDHSLESHGKAGRKRWFGRRP
                     RTRGVVMNPVDHPMGGGEGRASGGHPRSRKGVPAKGYKTRNPKKTTNKFIIERRRKRK
                     K"
     gene            1204..1470
                     /gene="rpsS"
                     /locus_tag="PLI69_01605"
     CDS             1204..1470
                     /gene="rpsS"
                     /locus_tag="PLI69_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOG41219.1"
                     /translation="MSRSLRKGPYIQDSLEKRILAMNEGSMKKEVIKTWSRSSMISPD
                     FVGHTIAVHNGNKFVPVYITENMVGHKLGEFAPTRTFKGHSGNR"
     gene            1493..1924
                     /gene="rplV"
                     /locus_tag="PLI69_01610"
     CDS             1493..1924
                     /gene="rplV"
                     /locus_tag="PLI69_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOG41220.1"
                     /translation="MGSRKREMAERLKEIKKQTAIAKLNDIPTSPRKMRLVADIIRGV
                     EVNRALDILKYSKKEASIRLEKLVRSAIANWEAKNEADRKELENGNVYIKSIMVGPGS
                     TLKRIRTAPQGRAARIRKRSNHVTIVLDTKKTVKPVTTEEA"
     gene            1927..2697
                     /gene="rpsC"
                     /locus_tag="PLI69_01615"
     CDS             1927..2697
                     /gene="rpsC"
                     /locus_tag="PLI69_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOG41221.1"
                     /translation="MGQKTNPISNRIGIIRGWDSNWYGDYASRILEDAKIREYLNTRL
                     AKASLSKIVIERTLKLITVTIHTARPGVIIGKGGQEVDKLKEELKKVCNKEVQINIFE
                     VKRPEVDANIVANNIARQVERRVSYRRAVKMAIASTMRVGAEGIKVLISGRLGGAEMA
                     RSELYKEGRTPLHTFRADIDYALAEAHTKVGVLGIKVWICNGEVYGKRDLSPNTGISA
                     NAASTQPQSRGGDRRGRSGRGGDRRRQSGRPRQSRDSE"
     gene            2848..4149
                     /locus_tag="PLI69_01620"
     CDS             2848..4149
                     /locus_tag="PLI69_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG41222.1"
                     /translation="MKRLVISAVTAAMMILTAICAGAQQNGTASKVLTLEECRTLARV
                     SIGNSQNASLVQQGAEIRGSILKRIILPQIAAVGYGTYQTHTPDMGALINHQIDFKKL
                     SQWQYGSYLFFAQNIYDGGEYRTKRLLNNLAKEAELLDIEQKALQMENIADELYFNAL
                     LAEKSLEILKLAHERLQSDLNSIKSLFAEGVIYRKDVMLIEMQLLESSHRISALEIER
                     EKCLALLSTLTGSEITTDTRLEMPSRELVDAVAEDPAMRKIDVRREQIDAGRRLSLSK
                     VLPRVELFAFGHYGKWGMDVFSDDPLLSGMAGLALIIPISDWRTHHRNKALSVNEKNL
                     LEITRANLELQTELKRTEIEKEVSAYEELEKQTAEMASKYRELADELRLMMQKGEVSE
                     NEYLTALNMQTQTELNREYHRIEKIRNIILSHRIFIDNLDN"
     gene            4155..5042
                     /locus_tag="PLI69_01625"
     CDS             4155..5042
                     /locus_tag="PLI69_01625"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOG41223.1"
                     /translation="MKHLSIILTATLLISACNQYKEPDGYGIFYNIEWTVSADADGKV
                     TDLGAEEGTILRSGEVVGHIDTTLLVLQRNNLISQMAALRATLPKEAIQIEAYISEKE
                     ALVNELERVRNLVESGSLDSKKMEQTQDKISILESRIEAARAQIKQQSASVLAQIELL
                     VSQKHLIDEQIRRSTIYSPEDGIVVTKYLNEHEYVLTGRPIFKLARTDKLIFKCWLDG
                     ATLTKVNLGDSVTVMVDGPDGTLVEHQGIVSYISEHSEYTPNHIQTRDNRTRLVYMVK
                     IDVPNDGTLRAGLPAELHL"
     gene            5039..5749
                     /locus_tag="PLI69_01630"
     CDS             5039..5749
                     /locus_tag="PLI69_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG41224.1"
                     /translation="MIHAEGISKWYGSFQALYDLTFSVEKGTIISMIGANGAGKSTLF
                     DIITTLDSDFKGKMMVGGYDTRREKSAIRKLIGYVPGTFSLYSDLTVMENVDFFANAY
                     GCGREALYKMTPRIWSSLEPFAGYRASQLSGGMKQKLTLCCAAVHSPSILLLDEPTTG
                     IDPLSRWELWQELLALREQGITILMSTHYLDEAARADKVMLLDKGRLISYDTPKHLTE
                     DEESLEEVFIKFLERRDE"
     gene            5742..6428
                     /locus_tag="PLI69_01635"
     CDS             5742..6428
                     /locus_tag="PLI69_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG41225.1"
                     /translation="MNRILTISSLNKRFGSLLAVRDFSLTLDKGEVLGIFGANGAGKT
                     TLIRMIIGLTKPTSGEYIVATGSRIGYMSQSFSLVGTLTVLENFEFYGALYNLPWQEF
                     ITQRDRFINHFKMDEYLNVRANRLPAGRKQLLAFSISRLHSPTLLLLDEPTSGLDLLN
                     RKRLWEAVRDAANEGIAILVSTHYLDEAFHCDNMVMMEHGAIKAYGKPVDLAAKAGGD
                     LINLFLEEMK"
     gene            6425..7507
                     /locus_tag="PLI69_01640"
     CDS             6425..7507
                     /locus_tag="PLI69_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG41226.1"
                     /translation="MRGVWTVARKELSHILRDKVSGLILFALPALFFISIGFAISIDI
                     QRIDLAIANTAGPKANEVISRLCDLEKVNKVVMLNSPSEVEESFARDEVSAAIVISEG
                     EGGYNAPLKFDLFVDCVFTGNVLAIKNRINSVIKSSLIGPENDPPFTIRYLYNADLNK
                     RMATIPGLIMIILIVVSTMIFGISINREKVNGTSRLLSLTPLGTVSIVMGKIIPYFLI
                     SLVHISYLIFLGQSVFGLGINGSFLLYFLFCALFSLNSMMIGLLVGSYCKTYEATLII
                     CWFFIFTPNLFLSGFVMPVNNMPEAMRAISRLCSGYHFIEGYRGIAYKGTGFTENLPF
                     ISFLTIQSLVALILALLGFSRRYSKS"
     gene            7507..8577
                     /locus_tag="PLI69_01645"
     CDS             7507..8577
                     /locus_tag="PLI69_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG41227.1"
                     /translation="MRRLYYIFKHEVLTFVRNPLMILLLVLSPLVVVGFIPQTLGNTN
                     KVSCAIVDMDRSAASREIVQIMLSAEDCLSPALCGSIEEAMRLMERNKVAMIIFIPDG
                     YGRDMDLGHPRKMTLLADGARVLEADISASQLISLLSGDQLSNGIVSNHRLFNNYDHS
                     EEFYLVSLMSLTLVFIAIFLIGLNLSEEKKHNTNSQIIVTGVDMRLYLGIKVLFHSLV
                     VILELFICLLLGYIVYDMTIVSGLGQLILVTYILTFPLLFVGVFLAAVARNDIQTIYL
                     MIFAMIFLVMLSSIIAPLINMSGISYYFCRLNPVYWLTKGLRTVITYGFPLRTLIPEL
                     VAGIVLSLIFFFSSIRLLKRIN"
     gene            8615..>9010
                     /locus_tag="PLI69_01650"
     CDS             8615..>9010
                     /locus_tag="PLI69_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOG41228.1"
                     /translation="MKRFLLIALFGAFLATGCTTTPAPGTLAKATPESQGMNPAILSQ
                     VDSVVFKAIEEGQCPGAVLSVVKGDKIVYLKAYGNKSVVPDTVAMTTETVFDLASVSK
                     CVGTTLSILQLVENGSIRLTDRVDIYIPDF"
BASE COUNT         2537 a         2050 c         2118 g         2305 t
ORIGIN      
        1 gttgacggag ttcaggatat tctggctgaa agacgcaaat aacaaaagaa cgatgggaat
       61 tttaattaag ccagtagtaa ctgaaaagat gacgattcaa ggcgataaat tgaatcgata
      121 cgcttttatc gttgaccgcg acgccaacaa ggtggagatc aaaaaagcgg ttgaggaaat
      181 gtatggtgtt tcagtaattg acgtaaacac tgtaaattac cacggcaaac gtaaaagccg
      241 ctatacaaca gcaggaatgc tgacaggccg tacaaatcac tataagaagg catttgtgac
      301 attggccgga gatgataaga tagacttcta tagtaacatt taaggccatg gcagtacgta
      361 aattcaaacc ggtaactccc ggacaaagaa ataaggtgat cagcgctttt gacgatatca
      421 cctgcacaac ccctgaaaag tcccttctta agcccatcaa gcgcacgggt ggacgcaaca
      481 accagggtaa gatgacaatg cgctatatag gcggcggtca caagagaatg tatcgagtaa
      541 tcgacttcca tcgtgacaaa gaccaatata cagctactgt caagactatc gaatacgatc
      601 caaaccgtag tgcacgcatc gcattggtcg tatatggtga tggcgaaaaa cgctacatca
      661 tcgctccaaa cggtatcaag gtaggccagg tgatctcttc cggtcccggc gtagcccctg
      721 aaatcggtaa cgctcttcct ctatcggaaa ttcctctcgg tacattggta cacaacattg
      781 agctccgtcc gggtcatggt gccgccatgg cacgcagcgc cggcactttt gctcaactta
      841 cagcacgcga aggcgaccat gcaatacttc gtctcccttc aggtgagaca cgtatggtac
      901 tggtaacctg ccgcgctact gtgggtactg tgtccaatcc ggaccacagc ctggagtctc
      961 atggtaaagc cggtcgcaaa cgctggttcg gtcgcagacc ccgcacccgc ggtgtcgtaa
     1021 tgaatcccgt ggatcaccct atgggtggtg gtgaaggccg tgcatccggt ggacaccccc
     1081 gttcacgcaa gggtgtgcct gccaagggat ataagactcg caatcccaag aagactacca
     1141 ataagttcat cattgaaaga agaagaaaga gaaagaaata acggaattaa actttagaag
     1201 attatgagtc gttcacttag aaaaggtcct tatattcagg acagtttgga aaaaagaata
     1261 cttgccatga atgagggcag tatgaaaaaa gaggtcataa agacctggtc aaggtccagt
     1321 atgatttcgc cggactttgt gggacacact atcgctgttc acaacggaaa caagtttgtt
     1381 ccggtatata ttacagagaa tatggttggt cacaaactcg gagaatttgc accgacacgc
     1441 accttcaaag gtcactcggg taatcgttaa catttaaaaa gagaagatta caatgggatc
     1501 tagaaaaaga gagatggccg aaaggctgaa agaaataaag aaacagacag ctatcgcaaa
     1561 gctgaatgat attcctactt cgcctcgtaa gatgcgtctg gttgctgaca ttatcagagg
     1621 tgtagaggtc aaccgtgctt tggatattct caaatattcg aagaaagagg catctatccg
     1681 cctcgagaaa ctcgtacgca gtgcaatcgc caactgggag gccaaaaatg aggcagaccg
     1741 caaagagttg gagaatggca atgtatatat caagagcatt atggtgggac ccggaagtac
     1801 gctaaaacgt atccgtacag cccctcaagg ccgtgcagct cgtatacgta agcgttcaaa
     1861 ccacgttacc atcgtgcttg ataccaagaa aaccgttaaa ccagtaacaa ctgaggaggc
     1921 ataaaaatgg gacagaaaac taaccctatc agcaatcgta taggcattat ccgtggctgg
     1981 gactccaact ggtacggtga ctatgccagt agaattctcg aagacgcaaa gattcgtgaa
     2041 tatctgaata ctcgtcttgc aaaagcaagc ttgtcaaaaa tcgttataga gcgtactttg
     2101 aaactcatca ctgtaaccat ccacaccgct cgtccgggtg tcataatcgg caaaggcggc
     2161 caggaggttg acaagttgaa ggaagaattg aagaaagttt gcaataaaga ggtacaaatc
     2221 aacatctttg aggttaaacg ccctgaggtt gatgccaaca ttgttgccaa caacatcgcc
     2281 cgccaggttg aaagacgtgt ttcttaccgc agagcagtca aaatggctat tgcatctaca
     2341 atgcgcgtag gtgcagaagg tatcaaggtg cttatcagcg gccgtctcgg cggcgctgaa
     2401 atggcaagaa gcgagctcta caaagagggc cgtactcctc ttcacacttt ccgtgctgac
     2461 attgactatg ctttggcaga ggcacacact aaagttggtg ttctcggcat taaagtatgg
     2521 atatgcaatg gtgaggttta tggcaaacgc gacctatcac ctaacacagg catttctgcc
     2581 aacgctgcat caacccaacc acaaagccgt ggcggcgatc gtcgtggtcg tagcggacgc
     2641 ggtggcgaca gaagacgtca gagcggacgt ccccgtcaat caagggatag cgaataataa
     2701 agaggttcga tatctaatat taaaaaatac tcaatattag atactcagaa atccaaactt
     2761 aaaacggcct tccaaggccg tttttttata tccggtattt ctcactattt ttgtttaaat
     2821 ttgtatttta gtaggtaaaa cagttttatg aagcgcttag tgatttcagc ggtaactgcc
     2881 gctatgatga tattgacagc tatttgcgcc ggagcgcaac agaatggcac agcgagcaaa
     2941 gtgctgactc tggaggagtg ccgtactctg gcccgcgtat ctattggaaa tagccaaaac
     3001 gcctcactgg tgcagcaagg tgctgaaata cgcggtagca tactcaaaag aataatcctt
     3061 ccacagatcg ctgcagtcgg ttatggaaca taccagacgc atacgccgga tatgggcgcc
     3121 ctgatcaacc accagattga tttcaagaaa ttatcccaat ggcagtatgg cagctatctg
     3181 tttttcgctc aaaatattta cgatggtggt gagtaccgta ctaaacgtct gctaaataac
     3241 cttgcaaaag aggcggaact gctcgatatt gagcaaaagg ctcttcaaat ggaaaatatc
     3301 gctgatgaac tctatttcaa tgcactactc gccgaaaaga gcctcgaaat tctcaaactt
     3361 gcccatgaga ggctccagag tgatttaaat agtatcaaat ccctttttgc cgaaggtgta
     3421 atctatcgca aggatgtgat gctaattgag atgcagttgc ttgagagcag ccataggata
     3481 tccgctttag agatcgaaag agaaaaatgt ctggcattac tctccactct gacgggaagt
     3541 gaaataacta ctgacacccg gctcgaaatg ccttcgcggg agttggtaga tgccgtagca
     3601 gaagatcccg ccatgcgtaa aatcgatgtg cgtagagaac agattgatgc cggcagaagg
     3661 ctttctctct caaaagtgct tccgagggtg gaattgtttg ctttcggcca ctatggcaaa
     3721 tggggtatgg atgttttttc agacgatcct cttctctccg gaatggccgg cctcgccctc
     3781 ataataccta tttctgactg gcgaacacac caccgcaaca aagccctatc cgtcaacgaa
     3841 aaaaaccttt tggaaatcac cagggctaac ctggaactac agaccgagct caaacgcacc
     3901 gaaattgaaa aggaggtatc agcctatgaa gagctggaga aacagaccgc tgaaatggct
     3961 tcaaaatatc gtgaactggc ggatgagctg cgcttgatga tgcaaaaggg agaagtctcc
     4021 gaaaacgaat atctcaccgc actcaatatg cagacacaga ccgagctcaa cagggaatat
     4081 catcgaattg aaaaaatccg caatattatt ctaagtcacc gcatatttat agacaacctt
     4141 gacaattgac gactatgaaa catctctcta taattctcac agccactctg ctgatctctg
     4201 cctgtaatca gtacaaagag cctgatggct atggcatttt ttacaatata gaatggaccg
     4261 tatctgccga tgcagacggg aaagtaaccg atcttggagc cgaagagggc acaatcctca
     4321 ggagcggaga ggtagtagga catatagata ccacccttct ggtacttcag cgcaacaacc
     4381 ttatctctca gatggctgcc ctaagggcca ctttgccaaa agaagcaata cagatagagg
     4441 cctatatcag tgaaaaagag gctctggtca atgaattgga aagggtacgc aatctggtgg
     4501 agtcagggtc gctggactcc aagaaaatgg agcagaccca agataagatt tccattctgg
     4561 agagcagaat agaggctgca agagctcaga tcaagcagca gtcagcctcc gtactggcgc
     4621 aaatagagct actcgtttct cagaagcatt tgattgacga acagatcaga cgctctacca
     4681 tatactcacc agaagatgga attgtagtaa ccaaatatct caacgaacac gagtacgtgc
     4741 ttaccggtcg cccgatattc aaacttgccc gtaccgacaa gctgatcttc aagtgctggc
     4801 ttgacggagc taccctgaca aaggtcaatt tgggtgacag cgttaccgta atggtagatg
     4861 gtccggatgg cactctggtg gagcatcagg ggattgtaag ctacatttcc gaacactcgg
     4921 aatatactcc caaccacata cagacccgtg acaaccgcac ccgtcttgta tacatggtaa
     4981 aaattgacgt gcccaatgac ggcacactgc gcgccggtct acctgctgaa ctccacttat
     5041 gatccatgcc gaaggcatat ccaagtggta cggcagcttt caagcgttgt acgatctcac
     5101 cttcagtgtt gagaaaggga ctattatctc catgatagga gccaacgggg cgggcaagtc
     5161 caccctgttt gatatcatta ccactcttga cagtgatttc aagggaaaaa tgatggttgg
     5221 aggatatgat accaggcggg agaagagcgc catacgcaaa ctcatcggat atgttcccgg
     5281 caccttttcg ctctatagcg acctgaccgt tatggagaat gtggatttct ttgcaaacgc
     5341 ctacggatgt ggcagggagg cactctataa gatgactccg cgcatttgga gttctctgga
     5401 gccatttgcg ggataccggg cgagtcaact ctccggaggc atgaagcaaa agctcaccct
     5461 ctgttgtgcc gcagtccact ctccttcgat actgctgctc gatgaaccca ctaccggcat
     5521 agatccccta tcaagatggg aactatggca agaattgttg gctctgaggg agcagggtat
     5581 cacaatattg atgagcacgc actatctgga tgaagctgca cgcgcagaca aggtgatgct
     5641 gctcgacaaa gggagactca tctcatacga cactccaaag catctgacag aggatgagga
     5701 gagtctggaa gaggtcttca ttaaattttt ggaacgcagg gatgaatagg attctcacca
     5761 tatcatccct taacaagcga ttcggctccc tattggccgt acgcgatttc tcccttacgc
     5821 tggacaaagg agaagtccta ggcattttcg gcgccaacgg tgccggtaaa acgaccttaa
     5881 tcaggatgat tatcggcctc acgaagccca cctccggtga gtacattgtg gcaacaggga
     5941 gtcgcatcgg ctatatgagc caatcatttt ccctggttgg gactctgacg gtattggaaa
     6001 attttgagtt ttacggtgcc ctctacaatc ttccttggca ggaattcata actcagagag
     6061 atcgtttcat caaccatttc aagatggatg aatatctgaa tgttcgtgcc aacaggttgc
     6121 ctgccggcag aaaacaattg ctggccttct ccatatcgag gctccactct cctactctgt
     6181 tgctgctgga cgagcccact tcgggacttg acctgctcaa ccgtaaacgc ctctgggagg
     6241 ccgttcgcga tgctgccaat gaaggaattg ccattctggt aagtacccac tatcttgacg
     6301 aggcatttca ttgtgacaat atggtgatga tggagcatgg agctataaag gcctacggca
     6361 agcccgttga tttggcggcc aaggccggag gagacctaat aaacttattt ttggaggaga
     6421 tgaaatgaga ggcgtttgga cagtcgcacg aaaggagttg tcgcacatct tgagggataa
     6481 ggtgagcgga cttatcttgt ttgcactgcc ggcgcttttt ttcatctcaa taggctttgc
     6541 catttcgata gatatacagc gtattgacct ggccattgca aatactgcag ggccgaaagc
     6601 gaatgaagtg ataagtcgct tgtgcgatct ggagaaagtg aataaagtgg tgatgctgaa
     6661 ttccccctct gaagtggaag agtcttttgc acgggatgaa gtatctgctg caattgtcat
     6721 aagtgaaggt gagggtggct acaatgctcc cctcaaattt gatctctttg tagactgtgt
     6781 cttcaccggc aatgttcttg cgataaaaaa ccgcattaac tccgtcatca aaagctctct
     6841 tataggaccc gaaaacgacc ctccttttac cataagatac ctttataatg cagacctcaa
     6901 caaaaggatg gctaccattc cggggttgat tatgattatc ctgatagtgg tatcgactat
     6961 gattttcggc atcagcatca acagggagaa ggtaaatggc acatcacggc tgctttcact
     7021 tacaccactc ggaactgtga gtatcgttat gggtaaaatt attccctatt ttttaatatc
     7081 gctagtacat atctcctatc tgattttcct gggacaatct gtgttcggac tcggcatcaa
     7141 tggcagtttt ctgctctatt tcctcttctg cgccctgttt tccctcaact ccatgatgat
     7201 aggactgctt gtcggctctt attgtaagac atacgaggca actctcatca tctgctggtt
     7261 ttttatattc acgcctaatc tattcttatc gggatttgta atgcctgtta ataatatgcc
     7321 cgaagcgatg cgggcaatct ctcgtctttg cagtggatat catttcatag agggatatag
     7381 ggggattgca tacaaaggaa cgggctttac agaaaacctg cccttcattt catttcttac
     7441 aattcagagc ttggttgctc tcattcttgc cctgctgggg ttctcacgcc gttattctaa
     7501 atcctgatga gaagattgta ttacatattc aaacatgagg tgttgacatt cgtgagaaat
     7561 ccgctgatga tacttttgct ggttctttct cctttagtgg ttgtcgggtt cattccacaa
     7621 accctgggta ataccaataa agtgagttgt gccatagtag atatggaccg ctctgctgcc
     7681 agtagggaga ttgtgcagat aatgctctcg gctgaggatt gtctgtcccc tgctttgtgt
     7741 ggctccattg aagaagcaat gcgccttatg gagcgtaaca aagtcgcgat gatcattttc
     7801 atacctgatg gttacggacg tgacatggac ctgggtcatc ccaggaaaat gactcttttg
     7861 gcagatgggg ccagagtact tgaggctgat atctcggcat cacagttgat ctcactccta
     7921 tctggagatc aactttcaaa tggaatcgtc agcaaccatc gtctttttaa caattacgat
     7981 cactccgaag agttttatct ggtctctctg atgtcactga cgctcgtgtt tatagctatt
     8041 ttccttattg gactcaatct ctcggaagaa aaaaaacaca acaccaattc ccagatcatc
     8101 gttacgggag tcgatatgcg actctatctg ggtatcaagg ttttgttcca ttcgctcgta
     8161 gtgattctgg aactttttat ttgtctgctt ttggggtaca tagtctacga catgactata
     8221 gtttcaggtc tggggcaatt gattttggta acatatatac tcacattccc attgttgttt
     8281 gtaggtgtat tcctcgcggc tgttgcccgt aacgatattc aaactatata tctgatgatt
     8341 tttgcaatga tctttctggt aatgctcagt tcaattattg ctccgttaat caacatgagc
     8401 gggatctcat actatttctg ccgtctgaac cctgtttatt ggcttacaaa aggccttcga
     8461 acggtcatta cttatggttt ccctttaaga acattaattc cggaacttgt cgcaggaatt
     8521 gttttgagtc tgattttctt tttctcttca attagacttc tcaaaagaat taattaactt
     8581 tgatggttat caattaatca gtaaacatac tttcatgaaa cgttttctcc ttatagccct
     8641 gttcggtgca tttctggcaa caggttgcac taccacaccc gcacccggaa cgcttgctaa
     8701 ggcaacgccc gagtctcagg ggatgaatcc ggcgattctt tcccaagtag attccgtagt
     8761 atttaaagct attgaagagg ggcagtgccc cggagcagta ttatcagttg tcaaagggga
     8821 taaaatcgta tatctcaaag catacggcaa caaatcggta gtaccggaca ctgtggcaat
     8881 gactaccgag accgtatttg acctggcttc agtcagcaag tgtgtcggca ctactttatc
     8941 tattctccag ctggttgaaa acggcagtat ccgtctcacc gaccgtgtag atatctatat
     9001 cccggatttc
//
LOCUS       DAOSIE010000026         8574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_7712_length_8574_cov_3.925461, whole genome shotgun sequence.
ACCESSION   DAOSIE010000026 DAOSIE010000000
VERSION     DAOSIE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7712_length_8574_cov_3.925461"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLI69_01655"
     CDS             <1..258
                     /locus_tag="PLI69_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HOG41229.1"
                     /translation="FGFNNPTRVEYKAINLSSLQAACEKHNLTTFDIDTMIAVGLAGK
                     NDLVKILGNGELTAKIDVTANAFSASAIKKIEEAGGTANKI"
     gene            264..1589
                     /gene="secY"
                     /locus_tag="PLI69_01660"
     CDS             264..1589
                     /gene="secY"
                     /locus_tag="PLI69_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOG41230.1"
                     /translation="MKRFFQTIVNIFKIEELRTRILYTLGLIVIYRLGSYIVLPGIDA
                     SQLANLQSQSSEGLLGLLNMFSGGAFGNASIFALGVMPYISASIVIQLLAVMLPFFQR
                     LQREGESGQRKMNQYTRYLTIAILAIQGPAYIANLSNQLPPEAFVATIGRGWYGIFVT
                     MVLIGGTMFVMWLGERITDRGIGNGISLIIMVGIVARLPMALWIEFTEKLHSTGGGIL
                     MFIVELLILFLVFVASIALVQAVRKIPVQYAKRVIGNKQYGGVRQYIPLKINAAGVMP
                     IIFAQALMLFPLIFSNFSATKGLAAVMSNTAGFWYNFVFAMMVVLFTYFYTAITVNPT
                     NMAEEMKKNGGFIPGVKPGRKTAEYIDTIMSRITLPGSIFLAVVAVLPAFAMMLGVNN
                     QFAAFYGGTSLLILVGVILDTLQQIESHLLMRHYDGLMKTGRLRGRSGM"
     gene            1614..2381
                     /gene="map"
                     /locus_tag="PLI69_01665"
     CDS             1614..2381
                     /gene="map"
                     /locus_tag="PLI69_01665"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOG41231.1"
                     /translation="MVKPKTEEELVIIRENALLVSKTLAEVGRHIAPEITTKELDFIA
                     EKYIREAGAEPGFLGYGGFPFTLCISLNDTVVHGFPSDYKLQEGDIVSVDCGTKYREF
                     NGDSAYTFAVGKIREEDKRLLEATKASLYLGIEQAVSGNRTGDIGFAVQSYVESLGYS
                     VVRELVGHAIGRNLHESPEVPNYGRRGKGVKLREGMVLCIEPMVNAGGKEIYLDENGW
                     AVRTVDGKKSAHFELMVAIRKGAPEILSTFEFIENNK"
     gene            2414..2632
                     /gene="infA"
                     /locus_tag="PLI69_01670"
     CDS             2414..2632
                     /gene="infA"
                     /locus_tag="PLI69_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOG41232.1"
                     /translation="MPKQQAIEQEGTVIEALSNAMFRVELDNGHVIVAHISGKMRMHY
                     IRILPGDRVRVEMSTYDLTKGRISFRYK"
     gene            2647..2763
                     /gene="ykgO"
                     /locus_tag="PLI69_01675"
     CDS             2647..2763
                     /gene="ykgO"
                     /locus_tag="PLI69_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOG41233.1"
                     /translation="MKVKASIKKRSEDCKIVKRKGRLYVICKKNPKFKMRQG"
     gene            2800..3180
                     /gene="rpsM"
                     /locus_tag="PLI69_01680"
     CDS             2800..3180
                     /gene="rpsM"
                     /locus_tag="PLI69_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOG41234.1"
                     /translation="MMARIAGVDLPNNKRGEIGLTYIFGIGRSSAKKILTDLDIDLDT
                     KVKDWNDDQITAIRTKIANEYKIEGELRSSVQLNIKRLMDIGCYRGIRHRIGLPVRGQ
                     STKNNARTRKGRKKTVANKKKATK"
     gene            3193..3582
                     /gene="rpsK"
                     /locus_tag="PLI69_01685"
     CDS             3193..3582
                     /gene="rpsK"
                     /locus_tag="PLI69_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOG41235.1"
                     /translation="MAKKPTTSKKRVVKVDAYGQAHISSTFNNVIVTITNSTGQVISW
                     SSAGKMGFRGSKKNTPYAAQVAAQDCAKVAYDLGLRKIKAYVKGPGAGRESAIRTIHG
                     AGIEVTEIIDVTPLPHNGCRPKGRRRV"
     gene            3615..4220
                     /gene="rpsD"
                     /locus_tag="PLI69_01690"
     CDS             3615..4220
                     /gene="rpsD"
                     /locus_tag="PLI69_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOG41236.1"
                     /translation="MARYTGAKTKIARKFGEPIFGPDKQFEKKNYPPGQHGQAKKRKK
                     TSEYGTQLAEKQKVKYTYGLLERQFRNLYGKAHRMKGRTGENLILLLESRLDNIVYRL
                     GIAPTRAAARQLVTHCHITRNGEVCNIPSTIVKPGELVGIRERSKSLEVIQDSLSRSS
                     NTYSWLEWDAAKYQGKYTNVPLRSEVPENINEQLIVELYSK"
     gene            4261..5274
                     /locus_tag="PLI69_01695"
     CDS             4261..5274
                     /locus_tag="PLI69_01695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOG41237.1"
                     /translation="MAILAFQKPDKVIMLDATDTFGRFEFRPLEPGYGITVGNALRRI
                     LLSSLEGFAITSIRIDGVSHEFDTIPGVIESVVDIILNLKQVRLRRMIEDVDSEVVNV
                     TVSGKQEFTAEDISKSLSSFKVLNPDLHICSMEPSVKLNIEMRIGKGRGYVPSEENKV
                     DNAPLGTIAIDSIFTPIKNVKYSVEDWRVEQKTDYEKLNIEITTDGSIHPKEALKEAA
                     TILIQHFWLFSDEKITLETTVEQDNKQLDEESMRMRHILLTKLSDMELSVRALNCLKA
                     ASIETFADLVSHQRTDLMKFRNFGKKSLNEIDMLMDRLKLNFGMDITKYNIEIKAQEN
                     EAQ"
     gene            5261..5917
                     /gene="rplQ"
                     /locus_tag="PLI69_01700"
     CDS             5261..5917
                     /gene="rplQ"
                     /locus_tag="PLI69_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOG41238.1"
                     /translation="MRHNRSINSLGRKSGHRKAMLANMASSLILHKRIETTLEKAKAL
                     RVYVEPLITKSKEDTTHSRRIVFSYLKQKEAVAELFRVVAPKIADRPGGYTRIIKNGF
                     RLGDGANMAYIELVDFNKAVLEADAPAKETKKASTRRSRSKKSADTSTEKVVDKNVEV
                     SAEEAEVKATKAAKPAAKKAAPKAASKAAPKATPKGAGKVAAKAPVKKVSAPRKTCNA
                     "
     gene            complement(6140..>7161)
                     /locus_tag="PLI69_01705"
     CDS             complement(6140..>7161)
                     /locus_tag="PLI69_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HOG41239.1"
                     /translation="MAGYNIVLVGCDENGNIDVEELREKAVANRDNLAGIMITYPSTH
                     GVFEVEIREICNIIHENGGQVYMDGANMNAQVGLTNPGYIGADVCHLNLHKTFAMPHG
                     GGGPGVGPICTAKHLTPYVPGHPVLPECGGINMTAVAAAPYGSPLLLPITHAYIKLLG
                     ADGLRKATEIAILNANYIAVQLEPELKTLYTGDKGRVAHECIVDFRHFRSEYGVDATD
                     IAKRLMDFGFHAPTLSFPVHETLMVEPTESESLPELDRFVEALKCIVAECEDIKAGRL
                     DKDDNPIKMAPHTACEVGSDNWSHPYTRKQAAYPLEWIRDNKFWPHVARVDSGYGDRN
                     LVATV"
     assembly_gap    7162..7171
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7172..>8574)
                     /locus_tag="PLI69_01710"
     CDS             complement(<7172..>8574)
                     /locus_tag="PLI69_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HOG41240.1"
                     /translation="NSSMLDDATAAAEAMKMMYELRSRDAVKEGKNVLFVDKNIFPQV
                     LSVLETRSAPLGIEIVLGDYSDYKFDEKCYGAILQYPAANGEVRDYSSFCSKAHERGI
                     LVTAYCDLLALAVLKEPAAWGADIAVGTAQRFGLPMGFGGPTAGFIATSDAFKRNLPG
                     RIVGISVDRHGNPAVRLALQTREQHIKREKATSNICTATALMATMSGMFALYHGPKGI
                     RKIAMNAYRHAHAVADTLRKNGYRLAAKNFFDTIEIIGAEGIEFDPAEINSRAEEAGI
                     NFCYTSDNTIIISFDELSNHEEASIIGRIFGCVDLNPGISAEPSFMKRETPILTSEIF
                     NKYHSETDMMRYIKKLERRDISLTHSMIPLGSCTMKLNAAVEVMPMSWSEFGNVHPFA
                     PTDQVEGSLKVIKELEHDLAVITGLDGCSLQPNSGAAGEYAGLMAIRAYHQANGEAHR
                     DIIIIPTSAHRTHPASA"
BASE COUNT         2378 a         1893 c         2067 g         2226 t
ORIGIN      
        1 tttggcttta acaaccctac cagggttgag tataaggcaa tcaatctctc ttctctacag
       61 gctgcttgtg agaaacacaa tctcactact tttgatattg acacaatgat tgctgtaggt
      121 ctggccggca agaacgatct cgtaaagatc cttggcaacg gtgaacttac cgctaaaatt
      181 gatgttacag ccaatgcgtt ttccgcttcc gccattaaga aaattgagga ggcaggtgga
      241 actgcaaata aaatctaatc attatgaaaa gatttttcca gacaatagta aacatcttca
      301 agattgaaga gctccgcacc aggattctct acaccctggg cctgattgtg atctaccgtc
      361 tgggaagtta tatcgtactt cccggtattg atgcatctca gcttgccaac ctgcagagcc
      421 agtcttccga aggtctgctg ggactattga atatgttctc cggtggtgct ttcggtaacg
      481 cttcaatctt tgcattgggt gttatgcctt atatttctgc ttccattgtc atccagcttt
      541 tggctgtgat gctccccttc ttccaacgcc tccagcgtga aggtgagagt ggacaaagga
      601 aaatgaatca gtatacacgt tatctgacaa tcgccatttt ggcaatacag ggtcctgctt
      661 atattgcaaa cctttccaac cagctccccc cggaggcatt tgttgctaca atcggaagag
      721 gttggtatgg gatttttgtc acaatggtgc tgattggagg tacgatgttc gttatgtggt
      781 taggtgaaag gattacagac cgcggcattg gtaatggtat ttccctaatc atcatggtgg
      841 gtatcgttgc acgtctaccg atggctttgt ggattgaatt cacggaaaag ttacactcta
      901 cgggaggcgg tattcttatg tttatcgtcg aactgttgat actatttctt gttttcgttg
      961 cctctattgc cctcgtgcag gcagtgcgta agattcccgt tcaatatgcc aaacgtgtta
     1021 ttggcaacaa acaatatggc ggagtgcgtc agtatatccc gttgaagatc aatgctgccg
     1081 gtgttatgcc gattatcttc gcacaggctt tgatgctctt cccgctcata ttttcaaact
     1141 tcagcgccac taagggcctt gcggcagtga tgtccaatac ggccggtttt tggtacaact
     1201 ttgtctttgc aatgatggtt gtcctgttta cgtatttcta caccgctatt acagtaaacc
     1261 cgaccaatat ggcagaagaa atgaagaaga atggcggatt tattccgggc gttaagccgg
     1321 gaaggaaaac cgccgaatac attgacacaa taatgtcacg cattacacta cccggttcta
     1381 ttttcctggc agtggtagct gttttgccgg catttgcgat gatgcttgga gtaaacaatc
     1441 agtttgcagc cttctacggt ggaacatcat tattgatcct cgttggcgtt atactggata
     1501 ctctccagca gatcgaaagt catttgctaa tgcgtcacta cgacggtctg atgaagacgg
     1561 gacgtctcag aggccgttcg ggtatgtaat tttattgata agttctttag agtatggtca
     1621 agcccaaaac cgaagaagag ttggttatca ttagggaaaa tgcccttctg gtttccaaaa
     1681 ctctggctga ggtcggacga catattgctc cggaaataac aactaaagag ttggatttca
     1741 ttgcggagaa atatatcaga gaggcgggag cagagccggg cttcttgggt tacggaggtt
     1801 ttccctttac attgtgcata tctctcaatg atacggtagt tcatggattt ccctcagact
     1861 acaaattgca ggaaggagat atcgtttcag ttgattgtgg cacaaaatat agggaattta
     1921 atggtgactc cgcctatacc tttgctgtcg gaaagatcag agaggaggac aagaggcttc
     1981 tcgaagccac gaaggcctcc ctctacctcg gtatagaaca ggcagtcagc ggcaatcgta
     2041 caggtgatat cgggtttgcg gttcagtcct atgtggagag cctcggttat tcagtggtca
     2101 gagagttggt cggtcatgcc atcggtcgca atctccacga aagccccgaa gtccctaatt
     2161 atggacgcag aggtaaagga gtcaaacttc gtgaaggtat ggtcctttgt atcgagccga
     2221 tggtcaatgc aggaggtaaa gagatttacc tggacgagaa tggttgggct gtacgtacgg
     2281 ttgatggcaa gaaatcggca cactttgagt tgatggttgc catcagaaag ggtgctccgg
     2341 agatcttatc cacatttgaa tttattgaaa acaacaaata accacaagtc aaaacctacg
     2401 gaattgttat aatatgccaa aacaacaagc tatagaacaa gaagggacag ttatcgaagc
     2461 tctttccaac gcaatgttca gagtcgaact tgacaacgga catgtgatag tcgcccacat
     2521 ttcgggtaaa atgcgtatgc attatatcag gatattgccg ggagataggg taagggtgga
     2581 aatgtccacg tatgatttga ctaaaggaag aatttctttc agatataaat aataaaactt
     2641 acacaaatga aagtaaaagc atccataaag aagcgtagtg aggattgtaa aattgtaaag
     2701 cgcaaaggtc gcttgtatgt aatttgcaag aagaatccaa aattcaaaat gcgccaggga
     2761 taatttttta atttgtaata acaataaagt aaaaaataga ttatggcacg tatagccgga
     2821 gttgatttac cgaacaacaa acgcggagag ataggattga cctatatctt cggtatcggt
     2881 cgcagttctg ctaaaaagat cctcacggat cttgatatag atcttgatac caaggtcaag
     2941 gactggaatg acgaccagat tacagcaatc cgtacaaaga ttgccaatga gtacaaaatt
     3001 gagggagagc ttcgctcatc cgttcagttg aacatcaaac gtctgatgga tatcggatgt
     3061 tataggggta tccgtcaccg tataggtctt cccgtacgcg gtcagagtac caagaataat
     3121 gcacgtacac gcaagggtcg taagaagact gttgcaaata agaagaaagc aactaaataa
     3181 gagatttgaa ttatggcaaa aaaacctaca acaagcaaga aaagagttgt taaagttgac
     3241 gcttatggtc aagctcatat ttcgtcgact ttcaacaacg tcatcgtcac aatcaccaac
     3301 agtaccggtc aggtaatcag ttggtcgtca gcaggtaaga tgggcttcag aggatccaag
     3361 aagaatactc cctatgcagc acaagtagcc gctcaagatt gcgccaaggt tgcgtacgat
     3421 ctcggattac gcaagattaa ggcatatgta aagggtccgg gagcaggtcg tgagtctgca
     3481 atccgcacta tccatggagc aggtattgaa gttaccgaga ttatagacgt tactcctctg
     3541 ccccacaatg gttgccgccc caaaggccgc cgcagagtat aaccttaata accttaaagc
     3601 aatagtataa taatatggca agatatacag gggccaaaac taaaatagcc cgcaaatttg
     3661 gagagcctat ctttggtccg gataaacagt tcgaaaagaa gaattaccct ccgggacagc
     3721 atggacaggc caagaaacgc aaaaaaacct ctgagtacgg tactcagctt gctgagaaac
     3781 aaaaagtaaa atatacttat ggccttctcg aaagacagtt ccgtaacctt tatggcaaag
     3841 cacacaggat gaagggccgc acgggcgaaa acctcattct cctcctggag tcccgtctcg
     3901 acaatatagt ttaccgtctc ggtatagctc ctacgagggc tgctgcaaga cagcttgtta
     3961 cacactgtca catcacccgt aacggtgaag tttgcaacat cccctcaacc attgtcaaac
     4021 cgggcgagct ggtaggaatt cgtgagcgct caaagagtct tgaggttatt caggatagcc
     4081 tttcacgctc ttccaacacc tactcctggc ttgagtggga cgctgcaaag taccagggta
     4141 aatataccaa tgttcctcta agaagcgaag ttcctgaaaa catcaatgag caacttattg
     4201 tagagttgta ctccaagtaa tccttgctta tagcgtaaac actaataaaa tttaaacagt
     4261 atggcaattt tagcatttca aaaaccggat aaggtaataa tgcttgacgc aacagacacc
     4321 ttcggtcgtt tcgaattccg tccgttggaa ccgggatatg gtataaccgt aggcaatgct
     4381 ctccgtcgta tactgctctc ttctttggaa ggctttgcca tcacctcaat aagaatcgac
     4441 ggtgtaagtc acgaatttga cactataccc ggtgttattg agagcgttgt tgacatcatt
     4501 ctaaacctca agcaggttag actcagaagg atgatagaag atgtggacag cgaggtggta
     4561 aacgtgactg ttagtggtaa gcaagaattt actgctgaag atatttccaa gagtttatcc
     4621 tcctttaaag tattgaatcc cgatctccac atttgctcca tggagccttc cgttaagctc
     4681 aatatcgaga tgaggatcgg taaaggaaga ggctatgtcc cttcagaaga aaataaagtg
     4741 gataatgccc ccctcggaac aattgcgatc gactcgattt tcactcctat caagaatgtg
     4801 aaatactcgg ttgaagactg gcgtgttgaa cagaagaccg actacgagaa gctcaacatc
     4861 gagattacca cagacggttc aatccacccg aaggaggcct tgaaagaggc agctaccatc
     4921 ctgattcagc acttctggct cttttcagat gaaaagatca ccctcgagac tacagtcgag
     4981 caagacaata aacaacttga cgaggagtcg atgcgtatgc gtcatatact tctcaccaag
     5041 ctttcagata tggagctctc agtaagggct ctcaactgcc tgaaagctgc aagcatcgaa
     5101 acatttgctg acttggttag tcaccagagg acagatctga tgaaattcag aaatttcgga
     5161 aagaaatctc tcaacgagat agatatgctc atggatagat tgaaactgaa cttcggaatg
     5221 gatatcacta agtataatat tgaaataaaa gcacaagaaa atgaggcaca atagatcaat
     5281 aaacagttta ggacgcaaga gcggacatcg caaagcgatg ctggccaata tggcctcatc
     5341 tctgattctt cacaagagga tcgagactac tctcgaaaag gctaaagcat tgagagtcta
     5401 tgttgaaccg cttattacca agtcaaaaga ggataccaca cactcacgtc gcatagtgtt
     5461 cagttacctg aagcagaaag aggctgttgc tgagttattc cgcgttgttg ctccgaagat
     5521 tgctgatcgt cccggcggat atacacgtat tatcaaaaac ggttttcgtc ttggagacgg
     5581 ggctaatatg gcatatatcg agttggtaga tttcaacaag gcagtccttg aagctgatgc
     5641 acctgcaaaa gagacgaaga aagcttctac tcgccgcagt cgctccaaga agagtgcaga
     5701 tacgagtacg gagaaggttg tggataagaa tgttgaggta agtgcagaag aggctgaagt
     5761 taaagcaacc aaggcagcca agccggcagc taaaaaagca gcccctaaag cagcttcaaa
     5821 ggccgctcct aaggcaactc ccaagggtgc cggcaaagtt gcggcaaaag ctccggttaa
     5881 gaaagtttcc gctcctcgca agacctgtaa cgcatagctt gagtagtgac gtaaaaacag
     5941 agatggccga aaaaatcggc catctctgtt tttattgagt atgtagtgtg tagtatgtag
     6001 tggtgctggt tgcagggtgc ggtttatgtg ttgcgtggcc accacgcacc ccgaaacgag
     6061 caatctgtac ctcacaccac tcaaagctcc ttactaagta ctttgtcaga actacacact
     6121 acttacttct gactcgaaac tatactgtag ctaccagatt gcggtcaccg tatccgctgt
     6181 ccacacgtgc aacatgaggc cagaacttgt tgtcgcggat ccactcaagg ggatatgccg
     6241 cctgtttgcg ggtgtaagga tgtgaccagt tatccgagcc aacctcacaa gccgtatgag
     6301 gtgccatttt gatgggattg tcatccttat cgagacgtcc ggccttaatg tcttcacatt
     6361 cggcaacgat acacttcaat gcttctacaa atctgtcaag ttccggtagt gattcgcttt
     6421 cggtaggctc gaccatcaat gtctcgtgta ccgggaatga aagtgtagga gcgtggaatc
     6481 cgaagtccat aagtcgcttg gcaatatcgg ttgcatccac gccgtactcg gaacggaaat
     6541 gtctgaagtc tacgatacat tcgtgggcaa cacggccttt atcccctgtg tagagggtct
     6601 taagttccgg ttccagttgc acagctatgt aattggcatt tagaatggca atttcagtag
     6661 ccttacgtag tccatcagct ccgagcaatt tgatataggc atgagtgatg gggagcagca
     6721 gagggctacc gtagggagcc gcagcaaccg cagtcatatt gatcccaccg cactccggga
     6781 gcaccgggtg gccgggaacg tatggagtaa gatgttttgc ggtgcaaatg ggacctacac
     6841 ctggaccacc gcctccgtga ggcattgcaa aggtcttgtg aaggtttaga tggcatacgt
     6901 cagcaccgat atagccgggg ttggtcagac ctacctgagc attcatatta gccccgtcca
     6961 tatagacctg accaccattt tcatggatta tgttgcaaat ttcgcgaatt tcaacctcaa
     7021 atacaccgtg ggtggaagga taggtgatca ttattcctgc caggttgtcg cggtttgcaa
     7081 ctgccttttc gcgaagctct tcaacatcaa tgttaccatt ctcatcgcat ccgacaagca
     7141 cgatgttgta gccggccatg gnnnnnnnnn nctgcagaag cggggtgggt gcggtgggct
     7201 gatgtgggaa ttatgatgat gtcacggtgt gcttcaccat ttgcctggtg ataggcgcgg
     7261 atagccataa ggccggcata ttcacccgca gcaccgctat tgggttggag cgaacagccg
     7321 tccagtccgg taatgacggc aagatcatgc tccaattctt tgattacctt tagcgaacct
     7381 tccacctggt cggttggtgc gaatgggtgt acattgccga attcactcca gctcataggc
     7441 atcacctcaa ctgctgcatt aagtttcatg gtgcagcttc caagaggtat catggagtgt
     7501 gtaagagaga tgtcccggcg ctcgagtttc ttgatatagc gcatcatgtc tgtctcagag
     7561 tggtatttat tgaatatctc tgaggtgagg atgggtgttt ctctcttcat gaatgaaggc
     7621 tctgcagaaa tgcccgggtt gagatctacg cagccaaata ttcttcctat tatggaagcc
     7681 tcttcatgat tcgagagctc gtcaaaactg atgattatgg tattgtcgga ggtgtagcag
     7741 aagttgattc ctgcttcttc cgctcttgaa ttgatctctg caggatcaaa ctcaatacct
     7801 tccgctccta tgatttcaat ggtgtcgaag aagttctttg cagcgagtct gtaaccattt
     7861 ttgcgaagag tatcagccac ggcatgagca tggcgataag cgttcatggc tattttacgt
     7921 atgccttttg gaccatgata tagtgcaaac attcctgaca tcgtggccat gagtgcagta
     7981 gcagtacaga tattggatgt ggccttctcc cttttgatat gctgctcacg tgtctggaga
     8041 gcgaggcgta ctgcggggtt gccgtgacgg tccaccgata taccgactat acggcccgga
     8101 aggttgcgtt tgaatgcatc tgaagtggcg ataaagccgg ccgtaggacc gccgaagccc
     8161 atgggaagcc caaatctctg ggccgtaccc actgcaatgt cagcccccca ggctgccggt
     8221 tccttaagta ctgcaagcgc aagaagatcg caatatgcag ttacgaggat accccgctca
     8281 tgagcttttg agcagaatga gctgtagtcc ctcacttcac catttgctgc cggatactgg
     8341 agaatggctc cgtagcattt ttcgtcaaat ttataatcag agtagtctcc cagcactatt
     8401 tcaatgccga gaggggctga tctcgtttcc agcaccgaaa gcacctgggg aaagatgttt
     8461 ttatctacaa agagtacgtt tttaccctct tttaccgcat cgcggctacg gagctcatac
     8521 atcatcttca tagcttcagc cgcggctgtc gcatcatcca acatcgagct gttt
//
LOCUS       DAOSIE010000027         8498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_7793_length_8498_cov_5.028781, whole genome shotgun sequence.
ACCESSION   DAOSIE010000027 DAOSIE010000000
VERSION     DAOSIE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7793_length_8498_cov_5.028781"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(533..3061)
                     /locus_tag="PLI69_01715"
     CDS             complement(533..3061)
                     /locus_tag="PLI69_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOG41241.1"
                     /translation="MKRILIFALGILFTSLLFAQQVPVTEPNYELADRFSAKKVNNMV
                     FSTYMRPNWFKNSDKFWYEWKTPAGSCYYMVDPVTKVQKPVFDLEKLAMELTEIVKDP
                     FDAQNIPFRKLKLKDDSKFTFEIQSTMMVEKKEKKKKEEVTNEVKNEEKQDEKKDEKK
                     EVKKEEKKATPKKERKVFRFEYDIASGKLTDVTENEEEKDYPRWAGISPDGKIAVYSK
                     QYNLYWMTIEDVKKLMEDEKDTTVVDHPLTTDGTKDFYYGGGSYRGSDQSDTSKRFSA
                     GVMWSPDSKHFAIGKYDMSKIKELWVINSVSQPRPTLESYKYQMPGEPGPKEHLLIFN
                     MEDKSSKEIRVDAFKDQQFDILAAPFKNRNAYDDYRVYTWLGDNSKFYLTRTSRDLKR
                     VDICAVNLAEDSCRVIIEERLNTYVETRRLEVINNGTELIQWSERNGWAHLYLYSSDG
                     TLKNPITSGAFHVESVLSVDEAARVVYFLAMGVNKDENPYNGHVYRINLDGTGMKRLD
                     ADGFNSSIVSFSDNSKYFVSNYSTVDVVPKVALFNAAGVKLLDVAETDISLLLEAGYK
                     FPEPFKVKAADGVTDLYGVMYKPFDFDPNKKYPIIDYVYPGPQTEGNNLVWSKGLTRV
                     DRLSQLGFIVITVGNRGGHPNRSKWYHNFGYGNLRDYGLEDQKYAIQQLAARHNFIDI
                     SRVGIHGHSGGGFMSTAAILQYPDFFKAAVSCAGNHDNSMYNRWWSEQHHGILEEITA
                     AGDTTFKYSIDKNQDIAKNLKGHLLLVHGDIDDNVHPGNTMRVVNALIRANKRFEMLI
                     LPGQRHGFGDMNEYFFWKMADWYSEWLLGTSKRHEVDIREMNND"
     gene            complement(3073..3579)
                     /locus_tag="PLI69_01720"
     CDS             complement(3073..3579)
                     /locus_tag="PLI69_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG41242.1"
                     /translation="MAYIGLISDTHGCFDDCLRGFLEPVDTIWHAGDFATLESALEIA
                     AFKPLVGVHGNCDGYDIRTYHPYIQLMDVEGLKVLMMHIGGYPGRYDLRALRLIEAHR
                     PNLFVCGHSHILRVINDKHYNMLTLNPGAAGIQGFHLVRTALRFRISDGKIHDMEVGE
                     WERNLTKQ"
     gene            complement(3586..4755)
                     /locus_tag="PLI69_01725"
     CDS             complement(3586..4755)
                     /locus_tag="PLI69_01725"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOG41243.1"
                     /translation="MTKAPTQRYVALDVLRGMTVAGMILVNNPGSWGHIFPPLRHAAW
                     SGCTPTDLVFPFFLFCVGVAMAFSFAKYGDAINKESSRKLVVRGLLIFAVGLALNMFP
                     FYPTSRNPELSFGANYLIWLQNIRIFGVLQRIGLCYILGGFLALWLKRPKRILLGIAA
                     VTVLHWLILWIFGGEPGWSTLEGNVASKIDIALVGESHVYRGFGIPFDPEGLLGVLSG
                     AGTVLLGYFIGQLIRSSENKIDAVAKLYTIALVCLGLGRIWNVWLPINKPLWTGSYVI
                     YTGGWAIMLFAFFIYFIDIKGKERYFTPFKAMGMNPLFAFVMAGVLVKLLGRVITWTN
                     AEGVKMSALSWFYKNCCVSFFGNNEYASLMHALVMVAIFLVMCMALYRKKIFIKL"
     gene            4840..5592
                     /locus_tag="PLI69_01730"
     CDS             4840..5592
                     /locus_tag="PLI69_01730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOG41244.1"
                     /translation="MEIFYSPDVARGGNRLDPIESGHCIRVLRHRQGDIINIVDGAGT
                     LFKCEITDDNPRCVVFAVLERQEGYGSHPYRLHIAIAPPKNTERFDWFVEKATEMGMD
                     EISPIFGDYSERRVFKRDRTERIMIAAAKQSHKGAIPVLHEPLTVREFLEANSSVKSG
                     KHLSEKAPLRLICYCDEVDSLGAVKISITEALKKGGSDVVVMIGPEGDFSREEMALAI
                     RLGWQPVSLGDSRLRIETAALTAVAAVYLANS"
     gene            complement(5628..6248)
                     /locus_tag="PLI69_01735"
     CDS             complement(5628..6248)
                     /locus_tag="PLI69_01735"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG41245.1"
                     /translation="MNKHIDNNKYSEAINAAIEVLKAGGVILYPTDTIWGIGCDATNS
                     QAVARVLSIKHRADSKSLITLVSDTDMLAKYVKEIPDIALDLIEVNDRPMTIIYPGAI
                     VATDSSPAGSALAPNVVSADGSVGIRIPDNPFCKALISHFGRAIVSTSANLSGNEAPA
                     NYSAISEEIINSVDWIADPSLESDSTGEASQIIKVGLRGEIAIIRG"
     gene            complement(6253..7326)
                     /locus_tag="PLI69_01740"
     CDS             complement(6253..7326)
                     /locus_tag="PLI69_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG41246.1"
                     /translation="MKRKKQRRFGLGSFISVTVLLISAAALLISYISIFISPSKFWPP
                     LFFGLYYIPIALWNLLLMIIAIIFRKKRLLIPLVALLPSLFFFDMFVKVGNEVPDEEK
                     EGICIMTYNVGRYALSKEGKSKNETITDIRDYLKLCDADIVCLQEVSVKYKDSLIELP
                     DYPYIYSHFFNDISSSFGNVILSKFPVRGGGKLTFKRSTNLAIWADLRIDGSIVRIYN
                     CHLESYSISFTSIVKRLFNRDTFSDEFISLHGHLKESNIKRAEQVDRVVGSISECRMK
                     TIVCGDFNDTPMSYTYNRLQKNHKDGFVEAGKGFGATYSRLWPALRLDYILIPEEYGT
                     TCHKIERLPFSDHYPVITQIKLE"
     gene            complement(7347..8012)
                     /locus_tag="PLI69_01745"
     CDS             complement(7347..8012)
                     /locus_tag="PLI69_01745"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG41247.1"
                     /translation="MNQGYSRPGFWGMLSPVIKNLIIINTVMLVATFIIGETMYEKLA
                     LFPFKSPFFKPYQLITHMFMHGDFWHLFFNMYTLMIFGTVLERIWGSKKFLLFYFVTG
                     IGAALCHSLVLHLQYDSLMTAGKIAAATAVLGTPTVGASGAIYGVLLGYGMLFPDNRL
                     QLIFPPIAMKAKWFVIIFGAIELITGLTGMGGNIAHFAHLGGMLFGLIMILIWKKQNR
                     LYH"
     gene            complement(8009..>8498)
                     /locus_tag="PLI69_01750"
     CDS             complement(8009..>8498)
                     /locus_tag="PLI69_01750"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41248.1"
                     /translation="RARERIWYERYLDLISDEKPLTQQTLFPRSIDLQSEDYLTLTEH
                     LDELSKLGFDIRDFGSNSVAVYGVPPGYSVAEGDTVALLDSLVASLKEQTFSADFAEY
                     GEALARKLAISAARSHAGPISNAQAQILVDELFDCKEPGLTPDGRKCMSVITVKDLEK
                     YL"
BASE COUNT         2220 a         1929 c         1993 g         2356 t
ORIGIN      
        1 acagtggttt atgataagac aaaccccgat aataacagac ccgagagaga gtattaggtg
       61 atagtcaatc ctcttatcgc attgttgatg gtttgtggtg gtccgaagat ataactaaac
      121 aaggtgcaag tagatttaag gtttttaaag aaactaataa cggactgaaa tggatttata
      181 atgccgataa gtacggagac tttatgaaaa atgaacataa aagcgtaaaa ggtattttta
      241 ttccttgggg gcaatttaaa acaataaaat aaaatggccg atattttgat agcgacaaca
      301 gaaaacttcc cgtttgattg ggcaaacatt gaatcttatc gagatagttt ttcttttact
      361 tccaccatac gggtagtatt ggtattaatt acaaaagtaa aaacaaatca ataaaaagta
      421 tattgtcaaa agcttctcgc accccgcacc ccggtacaac gtattagcgc gaattagcgc
      481 gaattagcgc aaaaggggct gactataatg tttttagtca gccccttttt agttagtcat
      541 tattcatctc tcggatgtcc acctcatggc gttttgaggt gccgagtagc cattcgctgt
      601 accagtctgc cattttccag aagaaatatt cgttcatatc gccgaagccg tggcgctggc
      661 cggggagaat cagcatctcg aaacgcttgt ttgcacggat aagagcattt acaacgcgca
      721 tggtgttacc agggtgcaca ttgtcgtcta tgtcgccatg aacgaggagc agatgacctt
      781 tgagattctt tgcaatatcc tggtttttgt caatgctgta cttgaatgta gtgtcgcctg
      841 ctgcggtaat ctcttcgaga atgccgtggt gctgctcact ccaccacctg ttgtacatac
      901 tgttgtcgtg gttgcctgca caagaaacgg ctgctttgaa gaaatccgga tactgaagga
      961 tggcggcggt tgacataaat cctccaccgg agtgtccgtg gatccccact ctgctgatat
     1021 cgatgaaatt gtggcgtgcg gccagttgct ggatagcata tttctgatct tccagtccat
     1081 agtcgcggag attgccgtag ccgaagttgt gataccattt ggatctgttg gggtgcccac
     1141 ctctgttacc taccgtgatt acaatgaatc cgagttgcga aagacggtca acgcgtgtca
     1201 gacctttgct ccataccaga ttgttgccct cagtctgggg accgggatat acatagtcga
     1261 tgatggggta ttttttgttg gggtcgaagt caaacggctt gtacattact ccgtagagat
     1321 cggtaacacc atcggcagct ttaaccttga agggttcggg gaatttgtag cctgcttcca
     1381 gaagcaggga aatgtctgtc tcggctacat ccagcagttt tacaccggct gcgttgaaca
     1441 gggctacttt cggtacaaca tccactgtcg agtagttgga tacaaaatat ttgctattgt
     1501 cggagaaact gactattgat gagttgaaac catcggcgtc gagacgtttc atgcccgtac
     1561 cgtcgagatt gatgcgatat acatgcccat tgtaggggtt ttcatcctta tttacaccca
     1621 ttgccagaaa atagacaact ctggcagcct catccacgct cagcacactc tccacatgga
     1681 aagcaccgga ggtgatgggg tttttgagtg ttccgtctga agaatagagg taaaggtggg
     1741 cccagccatt gcgctccgac cattggatga gttctgttcc gttgttgatt acctcaagcc
     1801 tgcgtgtctc tacgtaagtg ttgaggcgct cctcgattat taccctgcag ctatcttccg
     1861 ccagatttac ggcacagata tccactctct tcagatcgcg gctggtgcgg gtgagataga
     1921 atttgctgtt gtcaccaagc caggtataga cccgataatc gtcatacgcg ttcctgtttt
     1981 tgaatggtgc tgccagaatg tcgaattgct gatctttgaa ggcatccaca cgtatttctt
     2041 ttgaactttt atcttccata ttaaaaataa ggagatgctc ctttggaccg ggttctcctg
     2101 gcatctggta tttgtaactc tcgagagttg gacgcggctg ggatacggaa ttgataaccc
     2161 agagctcttt tattttactc atatcatatt taccgatggc aaaatgtttg gagtcgggcg
     2221 accacatcac acctgctgag aatcgtttgg aggtgtcact ttggtctgag cccctgtaac
     2281 tgccgccacc gtaatagaaa tccttcgtgc cgtcggtggt cagagggtga tccactacgg
     2341 tagtgtcctt ttcatcctcc atcagttttt taacatcctc aatggtcatc cagtagaggt
     2401 tgtattgttt gctataaacc gcgattttcc cgtctggaga gattcccgcc cagcggggat
     2461 aatccttctc ttcctcattt tcggtcacat cggtaagttt gcctgaagcg atatcatatt
     2521 caaatcggaa gacttttctc tccttcttcg gagttgcttt cttttcttct ttctttacct
     2581 cttttttctc atcctttttc tcgtcttgct tctcctcatt ctttacctca ttagtaacct
     2641 cctctttttt cttcttctct ttcttttcca ccatcatcgt actctggatt tcaaaagtaa
     2701 atttgctgtc atccttgagc ttaagtttgc ggaaagggat gttttgtgca tcaaaggggt
     2761 cctttacaat ttcggtcaac tccattgcaa gtttctcgag gtcgaaaacc ggtttctgaa
     2821 ccttagtaac agggtctacc atgtaataac aacttccggc cggagtcttc cactcatacc
     2881 agaatttgtc ggaattctta aaccaattgg gtctcatata ggtcgagaag accatattgt
     2941 tgaccttttt tgccgaaaaa cggtcggcca gttcgtagtt gggctctgtc accggtacct
     3001 gttgtgcaaa aagcagactt gtgaagagaa tgcccagagc aaaaatcaaa attcttttca
     3061 ttatagcgat tattattgtt tagttaaatt tctttcccat tcacctacct ccatatcgtg
     3121 gatttttcca tcggagatgc ggaacctcaa ggcggtgcgc accagatgaa atccctgtat
     3181 accggcagca ccgggattga gggtaagcat attgtagtgc ttgtcgttga tcactctgag
     3241 gatgtgcgag tgaccgcaga caaagagatt ggggcggtgt gcttctatca gccgcaatgc
     3301 tcgaaggtca tatctgcccg gataccctcc gatatgcatc atcagcacct tgagtccctc
     3361 tacatccatc agttggatgt agggatggta agtgcgtata tcatatccat cgcaattgcc
     3421 atgtaccccc actaagggtt tgaaagctgc tatctccaat gcggactcca atgtggcgaa
     3481 gtctccggca tgccagatag tatctaccgg ctcgagaaaa cctctaaggc agtcatcaaa
     3541 acaaccgtgt gtgtcagata tcagtccgat atatgccatc ggccgtcaaa gtttgataaa
     3601 aattttcttt ctgtacaatg ccatacacat cacaaggaag atggcaacca tcaccagtgc
     3661 atgcatcagc gaggcatatt cgttgtttcc gaagaatgag acgcagcagt ttttataaaa
     3721 ccacgagaga gcactcatct tgacaccctc cgcattggtc caggtaatca ccctgccgag
     3781 tagcttgaca agtactccgg ccatcacgaa ggcaaatagc gggttcatcc ccattgcctt
     3841 gaatggagtg aaatacctct cctttccctt gatatctatg aaatagatga aaaaagcaaa
     3901 cagcatgata gcccatccgc cggtgtatat aacatacgaa ccggtccata aaggcttgtt
     3961 tatcggtagc cagacgttcc agatgcgtcc caggccgaga cacaccagag caatagtgta
     4021 gagttttgca actgcatcga ttttattctc acttgagcgg atcaattgac cgatgaagta
     4081 tccgagcagt accgtaccgg cacctgatag cactccgagc agcccttccg gatcaaaggg
     4141 gatgccgaaa ccgcgatata cgtgactttc tcctaccaga gcaatatcaa tcttacttgc
     4201 aacatttccc tccagagtac tccagcccgg ctcaccgccg aaaatccaaa gaatcagcca
     4261 gtggagtacg gtaaccgcag cgatacccaa tagaatgcgc ttgggtcgct tgagccacaa
     4321 cgcaagaaat cctcccagga tatagcagag tccgatgcgc tgcaataccc cgaatatgcg
     4381 tatattttgg agccaaatca gataatttgc tccgaaagag agttcagggt tgcgggaggt
     4441 ggggtagaag gggaacatat ttagagccag acctaccgca aatataagca gacctctgac
     4501 tactagtttg cgtgaactct ctttattaat ggcgtcgcca tactttgcaa aggaaaaggc
     4561 catcgccact ccgacgcaga agaggaagaa gggaaaaacc aggtcggtcg gggtacatcc
     4621 cgaccatgcg gcatgtctca aaggggggaa aatatgaccc caggaaccgg ggttgttgac
     4681 aaggatcatt cctgctaccg tcattcccct gagtacatca agagctacgt agcgctgggt
     4741 tggagcttta gtcataagag agggatttgg attagtaccg ctgttcaact tacaaatata
     4801 gataaaatcc ctatctttgc ttagtcaaaa gggattgaaa tggaaatttt ttattcaccg
     4861 gatgtagcga ggggtggaaa ccgacttgat ccaatcgagt cgggtcactg tatcagggta
     4921 ctcaggcatc gtcaaggtga cataatcaat atcgttgatg gagcagggac tcttttcaaa
     4981 tgtgaaatca cagatgacaa tccacgctgt gtcgtttttg cagttctgga gcgtcaggag
     5041 ggctatggca gccatcccta cagacttcac attgcaatag ctccgcccaa aaatacggaa
     5101 cgcttcgact ggtttgtgga aaaggctacg gagatgggta tggacgagat ttctcccatt
     5161 tttggcgact attcggagag gcgtgttttc aagcgcgacc gtacggaacg cattatgatt
     5221 gccgctgcca agcaatctca taagggtgct atccccgtgc ttcatgaacc tttgacggtg
     5281 cgggaatttc tggaggccaa ttcatccgta aaatcgggaa agcacctctc cgaaaaggct
     5341 ccactgcggc tgatatgtta ctgtgatgag gtagactctt tgggtgcagt aaagatctca
     5401 atcactgaag cgttgaaaaa aggaggttcg gatgtggttg taatgattgg acctgaggga
     5461 gatttttcac gtgaggaaat ggctttggcg atccggttgg gatggcagcc tgtttcgctg
     5521 ggggattcac ggttgcgtat agagacggcg gcactgactg ctgttgcagc tgtttatttg
     5581 gccaactctt gattaacgta tcagttttta tctccggatt tggcttttca gccgcggatt
     5641 atagctattt cgcctctcaa tcccactttt atgatttgcg atgcctctcc ggtagaatca
     5701 gattcgaggg agggatccgc tatccagtct acactattga tgatctcttc ggagattgcg
     5761 ctatagttcg cgggagcttc gttaccggat agatttgcag aagtggagac gatcgctctt
     5821 ccgaaatggg atatgagggc tttgcaaaaa ggattgtcgg ggatacgtat tccaacgcta
     5881 ccatctgcgg aaacaacatt tggtgcaagt gcacttcctg caggggagga gtccgtagca
     5941 actatggccc ccggataaat aatggtcatc ggacgatcat ttacctctat cagatcgagc
     6001 gcaatgtcgg gaatttcttt cacatatttc gccagcatat cggtatctga gaccagtgta
     6061 atcagacttt tactatcagc gcgatgcttg atggataaga ctctggctac tgcctggctg
     6121 ttggtagcat cacatccgat gccccagatg gtatcagtgg gatagagtat cactccacct
     6181 gcctttagta cttctatggc cgcatttata gcttcgctat atttattatt atcaatatgt
     6241 ttattcacct ttttactcta gttttatttg tgtaattacc ggataatggt ccgaaaaggg
     6301 tagtctctct attttgtggc aagtggtacc atattcctca ggtatgagta tatagtccag
     6361 gcgcagggca ggccaaagtc gggagtaggt agccccaaag ccctttcctg cctctacaaa
     6421 gccatcctta tgattttttt gcaaccggtt gtaggtatat gacatcgggg tatcgttaaa
     6481 gtcaccgcag acaatggttt tcattctaca ttcactaatg ctgccaacaa cacgatctac
     6541 ttgttcggca cgttttatat tggactcttt tagatgtccg tggagagaaa tgaactcgtc
     6601 actgaatgta tctctgttga agagacgctt gactatggag gtaaatgaga tactatagga
     6661 ctccaaatgg cagttgtaga tgcgtactat gctaccatct atccgcagat ccgcccagat
     6721 ggccaggttt gtactccttt tgaaggtaag tttgccacct cctcttacag gaaatttact
     6781 gaggatgaca ttgccgaaac ttgagctgat atcgttgaag aaatgggagt agatataggg
     6841 atagtccgga agttctatta aagagtcttt gtattttacg ctcacctctt gaaggcaaac
     6901 aatatcggca tcacaaagtt tgaggtaatc gcgtatatcc gttatggttt cattttttga
     6961 cttgccctct ttactcaaag cataacggcc aacattgtag gtcattatac agataccctc
     7021 cttctcttca tcggggactt cattgcccac cttgacaaac atatcgaaaa agaagagaga
     7081 gggcagtagg gcaaccaaag gtatcagcag gcgttttttc ctgaagataa tggctattat
     7141 catcagaaga aggttccaca atgcgattgg tatatagtaa agaccgaaaa agaggggagg
     7201 ccaaaatttc gatggactaa taaagatgga aatataggaa atcaacaacg cggctgctga
     7261 gataagcagc acggtgactg agataaaact tcccaggccg aatctgcgtt gtttcttcct
     7321 tttcatagtt gcttagtagg agaggtctaa tggtagagtc tgttctgttt cttccaaatg
     7381 agaatcatta tcagtccgaa aagcattccg cccagatggg cgaagtgggc gatattacca
     7441 cccatccctg taaggccggt gattagttct attgctccga aaataatcac aaaccatttt
     7501 gccttcatcg ctataggcgg aaatatcaac tgcaagcgat tgtcggggaa tagcattcca
     7561 taacctaata acactccgta aatggctcct gaagcaccta cggtgggggt cccgagtacc
     7621 gcagtggctg ctgcaatctt accggccgtc atcagggagt catattgcag gtgaagtacg
     7681 agactgtggc acaatgcagc gccgattccg gtcacaaaat agaaaagcag aaattttttg
     7741 ctgccccaga ttctctccag cactgttccg aaaatcatca gggtgtacat attgaagaag
     7801 agatgccaga aatctccatg cataaacata tgtgtgatta actggtaggg cttgaaaaat
     7861 ggggatttaa aggggaaaag cgcaagcttt tcatacattg tttcaccgat aatgaaagtc
     7921 gcaacaagca ttacggtgtt gatgatgatc agatttttga taaccgggga gagcatcccc
     7981 caaaagccgg gacggctgta tccttgattc ataaatattt ctccagatct ttaactgtaa
     8041 taacagacat gcacttacgc ccgtccggtg tcagtccggg ctccttgcag tcgaataact
     8101 catccaccaa tatctgtgcc tgagcgttgg atatgggacc ggcatggctt cttgctgccg
     8161 aaatggccaa tttccgggct aaagcctcac catattctgc aaaatcggca gaaaatgttt
     8221 gttcttttag tgaagcgacc agcgaatcga gcagagcaac agtatctccc tctgctactg
     8281 aataacccgg agggactcca tatactgcaa ccgaattgct tccgaaatcg cggatatcaa
     8341 atcccaactt agagagttcg tccagatgct cggtgagggt gagataatcc tcgctctgca
     8401 gatcgatgct ccttggaaag agcgtttgct gggtcagcgg cttctcatcc gagatcaaat
     8461 ccagataacg ctcataccag atgcgctccc ttgccctg
//
LOCUS       DAOSIE010000028         8385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_7929_length_8385_cov_3.459664, whole genome shotgun sequence.
ACCESSION   DAOSIE010000028 DAOSIE010000000
VERSION     DAOSIE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7929_length_8385_cov_3.459664"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLI69_01755"
     CDS             <1..599
                     /locus_tag="PLI69_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOG41249.1"
                     /translation="VLASIKGTKYPERRYLLMGHFDHLGYMGIENVHPGANDNASGPS
                     MMLALAKYFSENPPESTIDFAFFDAEECNLLGSYYYKTNPRYPQENIRYILNYDMVAD
                     TPESIYLQVPEEGMAGYELIQKINMTFEEPFNFILDDIKDDSDNYFFALDGVPSLYFH
                     TEGKYNHIYHTPFDTYDNISDIGFERLFTLSINFIEKY"
     gene            688..2730
                     /locus_tag="PLI69_01760"
     CDS             688..2730
                     /locus_tag="PLI69_01760"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOG41250.1"
                     /translation="MLNHSYCSDKYQYTMGKSYYDGGMKDTIGIFNLFYRTAPDNNNW
                     AVVSGISEALEMIAGLGSENESFFEKFLPGDEYAEFRSYLAGMKFTGQVYAMKEGEIA
                     FPKQPIITVVGPIIEAQVLETPLLCIMNHQMAVATKASRVTRSTPKPVSEFGSRRAHG
                     PWAAQYGAKAAIIGGCQSSSNILAGIKCGFESSGTMAHSFTTSFGCSIEGEYKAFDTY
                     IKTHQGEPLILLIDTFDTLKCGLKNAIKAFKANGIDDSYAQGYGVRLDSGDLTYLSQK
                     SREALDEAGLTKCKIFATNSLDEYIISDLEKQNARIDIYGVGDAIATSKHNPCFGNVY
                     KLVQIGDEPVLKRSEEKIKLINPGFQITYRILQKGRFEADVTCLRGDSFARIIEAGEE
                     LTIVDEMDVTKFTTFPAGSYTFRKLQELVFDNGEIIPDTRTIQQKRDYYKANIACFDE
                     TQTRILNPHFYKVDISDDLYDLKMKLINRIILQINAMEEMEDTSGIKRCTHVVVDMLY
                     DFIDGSLACTNAEKAVEESVKYINAHPGQKVLYVLDNHPINHCSFKENGGIWPVHCVQ
                     KTRGGDIHAKFYRIENESNRPSRENKFYKGCNPRKEQYSGFEGRDREGIELNSRLSKD
                     VVISGIATEYCIRETCFDLIKSGHNVFLLEKALAYIDEKGHQKTLRELAKIGVKII"
     gene            2738..3688
                     /locus_tag="PLI69_01765"
     CDS             2738..3688
                     /locus_tag="PLI69_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG41251.1"
                     /translation="MNYIGIDLGGTKAVGAIFNSEGEILLQKNALLENRSGAEVGALI
                     CGLCKDLCEEGGISQGAPLSIGVCIPGISYSDTGCVWAPNIPGWENYPLRDELLEVVP
                     QATVTIESDRTCYILGEVQMGSAKGCRNAVFIAVGTGIGAGILIDNRVLHGHSDIVGA
                     IGWLALKPPYTDEYDECGCFETHCSGEGMAKQARRFLEEDKKYSGQLRNYRPEDITSY
                     HVFEAYDTGDPLAKRVIEEAIEMWGMATANLISIFNPEKVIFGGGIFGPAVKFIPQIR
                     REAEKWAQPISMKQVEITSTALPKLAGLYGAGAVAIKYAK"
     gene            3695..4843
                     /locus_tag="PLI69_01770"
     CDS             3695..4843
                     /locus_tag="PLI69_01770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG41252.1"
                     /translation="MTQKTNVKVIIAAQIGIFFFGVVFFVLGTILNALGLTPAQSSTL
                     ASSLPLGVLLGSLVFGPIIDRYGYKILLVIAAAMGVVGLEILAFTNSFGAMVAAILLI
                     GTCGGMLNGSTSALISDSSEGKARTSNLFVLGLVYCIGAIVITLIMTLLYDVVDYHKV
                     LMVMGFFMAAAMLYFIVIKFPEAKCKQGIAFKNVIGMIKEPTLLIFSFALAFQSGLEA
                     IAATWTSKYLADVGYVGKMALVASIFIPVGLAISRGSLIGISKKVSSRTIVLVSMAIA
                     VAGILALFSAESAIMAIIGTTLIGMGLAATFPATFGILGEKYSEMSGTAFSFALTIAL
                     IGNTLLNKLVGTLGTGSLPYVMLGSIVLLVVLFLIGYSTIKPEKVTKQ"
     gene            4858..7170
                     /locus_tag="PLI69_01775"
     CDS             4858..7170
                     /locus_tag="PLI69_01775"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOG41253.1"
                     /translation="MPSVASKRIVIVLLVVLSIVVISYGRSRYTITIGQNTIPAFRDT
                     IPVLQDTIPAVRDSAALSTKFPSTDTITAKSDTLTHPYGIMEIDTVDFWKHIDTLSIP
                     YLDSTYTAYFDSLALHLPDAKDIKRAERRIAREYRDSVRLATPRILDSYVIPDSLHYR
                     RMLTWTHDQDFNDITLTEIDTSFQYRFNDYPFLLNDLNGTYLGTIGSAMQYHNYFKRE
                     KLDVFPQFEPYLGYFHTPENMPQYNTKSAYTELAFWGTPFATQQLEESCVKILTTQNI
                     TPALNLSFVYRQYGSKGMLKNEATNNRSYAIGINYLGKKYLLNAGFISQAVIRSENGG
                     LQNSFWIRDTIVDSKSIEVNLQDADNLLKRRTAYLTHTYTIPMNFFRKKDSLAVGEGT
                     VAYIGHSAEYSRYGKVYTDNIAENDIHGRNFYFNQFHLNNTSSRDSLGTERFENKAFI
                     RIQPFAHDAVISKIDGGIGYQMLSIYSFDPSHYLSGGVNEHHNNLYFYAGASGQYRKY
                     LSWSANGKYTFAGYNLNDLDIGGKIKLSFYPIDDGIHLSGSLRTTLKEPHLFHQKIYT
                     NHHKWHNDFSKISESRVDVTLDIPKWQTHARFDYALVSGLIYYDTLSIIRQIEKPVSI
                     ISASLEQNVRLWILHLDNRALFQMASDGDALPLPKFSLNLKYYIEFNVVKDVMRMQIG
                     LNALFHTKYYVQSYSPDLGVFHNQKKELIGGTPYFDAFVNAQWKNVTVFVKYTNTFKG
                     WPQADYFSAYNYLRPERGFKFGIWWPFYIR"
     gene            7175..8191
                     /locus_tag="PLI69_01780"
     CDS             7175..8191
                     /locus_tag="PLI69_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG41254.1"
                     /translation="MIKKDVYFIGKGILLLLIVIAVRLIAPEHRVRRNTYHIYHKDTL
                     VFSILPDRKSRQGGFNRELIERFAEYNGSESFIIPPVGGEPDWKNLIEGKYDMLVFSM
                     NDSIPEQYASKVLVTRETGGKDACAVKVGNKRLLNSFNFWLSDFKSDDTYRQMVFRFF
                     RSYRVGAMPQGFDHTSAISPYDYVIKKYSNYINADWRLISAIIYEESRFVHSSSSTQN
                     ARGLMQIKESTAALYGVEDILDPELNIKAGTLHFDYLFNLYRKEGLDSANVIKFALAS
                     YNAGTGRIKQCRDHAASVGLDPNNWEEVVKGFDSMPYFKGTQTTKYVKNVIDLFERYK
                     QVVE"
BASE COUNT         2451 a         1925 c         1842 g         2167 t
ORIGIN      
        1 atgttctcgc ctcgatcaaa ggcaccaaat atcccgaaag gcgttatctg ctgatggggc
       61 atttcgacca tctcggctat atgggaattg agaatgtgca cccgggagcc aatgacaatg
      121 cttcagggcc aagcatgatg ctcgccctgg caaaatattt ctcagaaaat ccccctgagt
      181 ctaccatcga tttcgcattt ttcgatgcag aagagtgtaa tttgctcggg tcttactatt
      241 acaaaaccaa cccacgctat ccccaggaaa atatcagata cattctcaac tacgacatgg
      301 tagcagacac tccggagtcg atataccttc aggttccgga agagggtatg gcgggttatg
      361 aattgattca aaaaatcaat atgacatttg aagaaccctt caatttcata ctcgacgata
      421 tcaaggacga tagtgacaac tacttctttg cgcttgacgg agttccctcc ctctattttc
      481 atacggaagg caaatacaac catatctatc acacaccctt tgatacttac gacaatatct
      541 ccgacatagg tttcgaacgt ttgttcaccc tttcgatcaa ctttattgag aagtattgaa
      601 aatagcctga cagctctcca atttggtatt tagtacacta atcagtaact ttgttggttt
      661 attttaagtc ttaacacgaa ggatatcatg ttgaaccatt catattgttc tgacaaatat
      721 cagtacacga tgggaaaatc atactacgac ggtggtatga aggataccat cgggatattc
      781 aacctctttt acagaactgc ccccgacaac aacaactggg ctgtggtcag cggtatcagc
      841 gaagcacttg agatgattgc gggattaggc tccgagaatg aatctttttt tgagaaattt
      901 ctccccggag atgagtatgc ggagttccgt tcctatctgg caggaatgaa atttaccggt
      961 caggtatatg caatgaaaga gggtgaaata gcatttccaa aacaaccgat aattactgtt
     1021 gtaggcccca ttattgaggc acaagtactc gaaacgccac tactctgcat aatgaaccac
     1081 cagatggcag tggcaacgaa agcttcgcgt gtcacacgca gtactccaaa acccgtcagc
     1141 gaatttggct cacgcagggc gcacggccct tgggctgcac agtacggggc gaaggcggcc
     1201 atcataggcg gatgccaaag cagttccaat attctcgccg gcattaagtg cggatttgag
     1261 tcatccggca cgatggctca tagcttcacc acatcgtttg gttgctccat tgaaggtgaa
     1321 tacaaggcct tcgataccta tatcaaaacg catcaaggtg aaccgctaat actgctgata
     1381 gatacctttg ataccctaaa atgcggcctg aaaaatgcca taaaggcatt taaggccaac
     1441 ggcatcgatg actcttatgc tcagggttac ggggtgcgcc tcgacagcgg agacttgacc
     1501 tatctatctc aaaagtcccg tgaagctctt gatgaggcgg gactcacaaa atgcaaaatc
     1561 ttcgcaacca actccctcga cgaatacatc atatctgacc ttgagaaaca aaatgcccgc
     1621 atagatatat atggcgtggg tgatgctatc gccacctcaa agcataatcc ctgtttcggc
     1681 aatgtataca aacttgtaca gattggagat gaaccggtac tcaagcgctc ggaagagaag
     1741 ataaagctca taaaccccgg ctttcagatc acttaccgta ttcttcaaaa gggacgcttc
     1801 gaggccgatg ttacctgtct cagaggagac tcctttgcaa gaataataga agcaggagaa
     1861 gagcttacaa ttgtggacga gatggatgta accaagttta ccactttccc tgccggttcg
     1921 tacactttcc gtaaactaca ggaacttgta tttgacaatg gagaaattat cccggacact
     1981 cgcaccattc aacaaaagag agattactat aaggcaaata tcgcctgttt tgacgagaca
     2041 cagacccgta tcctaaatcc tcacttctat aaagtggata tatccgatga cctctatgac
     2101 cttaaaatga agctcataaa caggattatc ctacagataa atgcaatgga ggagatggag
     2161 gacaccagcg gcataaagag atgtacccac gttgtggtag atatgctgta cgactttatt
     2221 gacggctctc tggcttgtac caacgccgaa aaagcagtag aggagagcgt aaaatatatc
     2281 aatgcacatc ccggccaaaa ggtgctatat gtgctggaca atcatcctat aaaccattgt
     2341 tcctttaaag agaatggtgg aatctggcct gtacactgcg tacaaaagac acgcggagga
     2401 gatatacatg ccaaatttta ccgtatagaa aatgaatcca acagaccctc cagagaaaat
     2461 aagttttata agggctgcaa tccccgaaaa gagcaatact caggatttga gggacgtgac
     2521 agagaaggca tagaacttaa ttcccgtctc agcaaggatg tagtaataag cggcattgct
     2581 accgaatatt gcatacgtga gacctgtttt gatctgatta aatccggaca caatgttttc
     2641 ctgctggaaa aggcccttgc ctacattgat gagaagggac accagaaaac cttaagggaa
     2701 ctggccaaaa tcggagttaa aatcatataa cggagctatg aactacatcg gaatagatct
     2761 cggcggcact aaagctgtag gagcaatttt caacagtgag ggagagattc tcctccaaaa
     2821 aaatgctctc ctcgagaata gaagcggcgc tgaagtgggc gcactcatct gcggtctctg
     2881 caaggatcta tgtgaggagg gtggcatatc tcaaggagct cctctgagca tcggtgtctg
     2941 cattcccggt atatcctact cagatactgg gtgcgtttgg gcccccaata ttcccggatg
     3001 ggagaactat ccattgcgag acgaattact ggaagttgta ccccaggcta cagtaaccat
     3061 tgagagcgac cgtacctgtt acattctagg cgaagttcaa atgggtagtg ccaagggatg
     3121 tcgcaacgca gtttttatcg ctgtcggcac aggtatagga gccggcattc tgattgacaa
     3181 ccgtgtgctc cacggtcatt cggatatagt cggagctatc ggatggctag ctctcaagcc
     3241 cccctacaca gatgaatatg acgaatgtgg ctgttttgag acacactgct ccggagaggg
     3301 aatggccaaa caagcacgcc gttttctgga agaagataaa aagtatagcg gacagcttcg
     3361 caattatcga cccgaagata ttacctctta tcacgttttt gaggcatacg acactggaga
     3421 tcctcttgca aaaagggtca tagaggaggc aattgagatg tgggggatgg ccacggccaa
     3481 tctcataagt atattcaacc ccgagaaggt aattttcgga ggaggaattt ttggtccggc
     3541 ggttaaattt attcctcaaa tccgccgcga agcggaaaaa tgggcccaac ccataagcat
     3601 gaaacaggta gaaatcacat caacagcgct gcccaagctc gcaggactct acggagccgg
     3661 agcggtagcc atcaaatatg caaaataacg gattatgaca caaaagacta atgtcaaggt
     3721 aataattgcc gctcaaatcg gcatcttctt tttcggagtt gtgttctttg tactgggcac
     3781 aatcctcaat gcactgggac ttactcctgc tcagagctcg actctggcca gctcgctccc
     3841 cctcggggtg cttctaggtt cgctggtatt cggcccaatc atagataggt acggctataa
     3901 gatccttctg gttatcgcgg cggcaatggg ggttgtgggt cttgagatac ttgcatttac
     3961 caactccttc ggagcaatgg ttgctgccat actccttata ggtacctgcg gaggaatgct
     4021 taacggctcc acaagcgccc tgatttccga ctcttccgaa ggtaaggcca ggacatccaa
     4081 cctatttgta ctgggtctgg tctattgtat cggagccata gtcatcaccc tgataatgac
     4141 actcctctat gatgttgttg actatcacaa ggttcttatg gtaatgggat ttttcatggc
     4201 tgctgcaatg ctctatttta tcgtgataaa attccccgag gcaaagtgta aacaaggtat
     4261 agcatttaaa aatgtaatcg gaatgatcaa ggagccaact ctccttattt tcagtttcgc
     4321 tcttgcattc caaagcggac ttgaggcaat tgcggcgacc tggacctcaa aatatcttgc
     4381 agatgtagga tatgtaggta aaatggcact tgtggcatcg attttcatcc ctgtcggact
     4441 tgcaatcagc agaggatcct tgattggcat ctcgaaaaag gtctccagca ggacaatcgt
     4501 gcttgtgagt atggcaatag ctgttgcagg catccttgct ctatttagtg ccgagagtgc
     4561 catcatggcc atcataggga caacacttat cggtatgggc cttgcggcca catttcctgc
     4621 cacatttggc atactcggag agaagtacag tgagatgtcg ggcacggcat tcagttttgc
     4681 tctcaccata gcacttatag gtaacacact gctcaataaa cttgtaggaa cactgggtac
     4741 cggctcactt ccttatgtca tgcttggatc catagtattg cttgtggtat tattcttgat
     4801 aggatactcc accattaagc ctgaaaaggt gacaaaacag taattattga tttataaatg
     4861 ccttcggtag ccagtaaaag aattgtaata gtactgcttg tagtattgtc catagtcgtc
     4921 atctcctatg gacgcagccg ttataccatt actatcggtc aaaatacaat tcccgctttt
     4981 cgggacacta ttcctgttct tcaggataca attcctgctg taagagatag tgcagcactc
     5041 tctacaaaat ttccctctac cgatacaatt acagccaaat ccgataccct tacccacccc
     5101 tacgggatta tggagataga tacggtggat ttttggaagc acatagacac tctctccatc
     5161 ccctatctcg actccactta tactgcttac ttcgattcac ttgctctcca tcttccggat
     5221 gctaaggata tcaaacgggc tgaacgaaga attgccagag agtaccgcga cagcgtcaga
     5281 ctggcgactc cccgtatttt ggatagctat gtcatacctg attcgcttca ctaccgacgt
     5341 atgctgacct ggactcacga ccaggatttc aatgacatta cccttactga aatagacaca
     5401 tccttccagt acaggttcaa cgactatccc ttcctgctca atgatctcaa tggcacatat
     5461 ctgggcacca tcggttctgc aatgcagtat cacaactatt tcaaacgtga aaagctggat
     5521 gtgtttccac aattcgagcc atatctgggc tatttccaca caccggaaaa tatgccacaa
     5581 tacaacacca aatccgcata tacggaactt gccttctggg gaactccctt cgccactcag
     5641 cagcttgagg agtcctgtgt caaaatactg actacccaga atatcacacc tgcgctcaac
     5701 ctctccttcg tctacagaca gtatggatct aaaggtatgc taaagaatga ggctaccaac
     5761 aaccgttcat acgccatagg gataaattac ctcggcaaaa aataccttct caatgccggc
     5821 ttcataagcc aggcggtaat ccgttccgaa aacggcggac tgcaaaactc tttctggata
     5881 agagacacta ttgttgactc aaaatccatt gaagtcaatc ttcaggatgc cgacaatctg
     5941 ctcaaacgaa gaactgcata cctcacacat acttacacta tcccgatgaa tttcttccgt
     6001 aaaaaggact cactggcggt tggagaaggt accgttgcat atatcggaca tagtgccgag
     6061 tattcgagat atggtaaggt ctacactgat aacattgcgg aaaatgacat acacggtcgc
     6121 aatttctatt tcaaccagtt ccatctaaac aatacatcca gcagagactc tcttggaacg
     6181 gaacggtttg aaaacaaagc attcatacgt atacagccct ttgctcacga cgccgttata
     6241 tctaaaatcg atggtggcat aggataccaa atgctctcca tatacagttt cgacccttca
     6301 cactacctta gtggaggcgt caacgagcat cataacaact tatactttta tgcaggagcc
     6361 tcaggacaat atagaaaata tctctcatgg agtgccaacg gcaaatatac tttcgcaggg
     6421 tataatctga atgaccttga cattggagga aaaattaaat tatcattcta ccctattgat
     6481 gatggcatac atctttcggg cagtctgcgt acaacactga aagagcctca cctttttcat
     6541 cagaagatct ataccaacca ccacaaatgg cacaatgact ttagcaaaat atctgaaagc
     6601 agggttgatg taactctgga tatcccaaaa tggcagacac acgccaggtt tgattatgca
     6661 ctggtgagtg gacttattta ttatgacact ctttcaataa tcagacaaat agagaagccg
     6721 gtcagcatta tttctgcttc tttagaacaa aatgtcagac tttggatact ccatctggat
     6781 aatcgggccc tgttccagat ggcatctgac ggggatgcac ttcctttacc taagttttcc
     6841 ctaaatctca aatattatat agaattcaat gtggtcaagg atgtaatgag aatgcagata
     6901 ggtctcaacg ccctgttcca caccaaatac tatgttcaat cgtacagtcc cgacctcgga
     6961 gtattccaca atcaaaaaaa ggaactcata ggaggtaccc catatttcga cgcatttgtc
     7021 aatgcgcagt ggaaaaatgt aaccgtattt gtaaaataca ccaacacatt caaagggtgg
     7081 cctcaggcag attatttttc cgcctacaac tacttaaggc ctgaaagggg cttcaaattt
     7141 ggcatctggt ggccattcta catcagataa atgcatgatt aaaaaagatg tttacttcat
     7201 agggaaaggt atactgctcc tgctgatagt gatcgctgta agactcatag ctccggaaca
     7261 tcgagtaaga cgcaatactt atcacatata tcacaaggat actcttgtgt ttagtatctt
     7321 acctgaccgc aaaagccgtc agggaggatt taatcgtgaa ttgatcgaga gatttgcaga
     7381 atacaatggc tctgagtctt ttataatacc ccctgtcgga ggagaaccgg attggaaaaa
     7441 tttaatcgaa gggaagtatg atatgctggt tttcagtatg aacgattcaa ttcccgagca
     7501 atatgcttca aaggtgcttg taactagaga aaccggcggc aaagatgcct gtgcagtaaa
     7561 agttggcaat aaacggctac tgaacagctt taatttttgg ctttccgatt tcaagtcgga
     7621 tgatacctat aggcagatgg tctttagatt tttcaggtca taccgtgtgg gtgccatgcc
     7681 gcaaggattt gatcacacga gtgccatttc tccttacgac tacgtaatca agaaatacag
     7741 taactacatc aatgcggact ggaggcttat cagcgcaatt atttacgaag aatcgcgttt
     7801 tgtccactct tcctcatcga cacagaatgc acgtggtctg atgcaaatca aagagtccac
     7861 cgcagccctt tacggtgttg aggatattct tgaccccgaa ctaaatataa aagcgggaac
     7921 gctccacttc gactaccttt tcaatctata ccgaaaagag ggcttggact cggctaatgt
     7981 gataaaattt gctctcgcat cctacaatgc gggtaccgga cgcatcaaac aatgtcgtga
     8041 tcacgctgca tcagtaggat tggacccgaa taattgggaa gaagttgtga aaggcttcga
     8101 ctctatgccc tacttcaagg gaacccagac cacaaagtat gtaaaaaatg tcatagacct
     8161 cttcgaacgc tacaaacaag ttgtggaata gtgtggagtg gggagtgggg agtctgtagt
     8221 agggagtgtg gaatgggtac tgtggagttt tgcggggtgc gggttaattc acccgcatcg
     8281 ttttgtccgg gcttacgcgg cttatgaaga gagtggatat tgagataatt agcatgatca
     8341 ctactgccga aatgatattg agcaaaagaa tatccacgaa agaga
//
LOCUS       DAOSIE010000029         8382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_7936_length_8382_cov_3.647652, whole genome shotgun sequence.
ACCESSION   DAOSIE010000029 DAOSIE010000000
VERSION     DAOSIE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7936_length_8382_cov_3.647652"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="PLI69_01785"
     CDS             <1..1305
                     /locus_tag="PLI69_01785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG41255.1"
                     /translation="QTDKLFIMNFGTLLKRILFSAILAILSSTLTAQRQGIFSIVTCP
                     AENTSKAMNISWGADTTITECIIEYTLARDTQWKESRTMEETSHLCTVFDKVYSKKAD
                     GTDFYEDAIFLKYEAALEELKPRREYKYRIVSERCTSDTYKFRTSGSNRWSACIIADF
                     HVYPPLRSRLDNAMAMMDKVQEYDPGFDWVLHLGDIISWGGSYSFWQEMYEEEHFKNY
                     MWAGVNGNHDNMSRGYIKNTNEYFKEANFYPRNGYKGEEGVCYWFYWGDALFIMLNNE
                     SMREEEGLNAAQEWVREVVKNNPARYRVVCEHYQWFFGDSGKTSQYGRWSDLFDELGI
                     DLALAGNNHIYVSTNAIYQGKESDGKKGTVYIQAPSSDNERGVPTPNPLTENLDKIRF
                     RWSEGAMTVGAIHLDVNKKRMVLTLHDRYGNILDKAEVKSKK"
     gene            1344..2150
                     /locus_tag="PLI69_01790"
     CDS             1344..2150
                     /locus_tag="PLI69_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG41256.1"
                     /translation="MKKRKKRIIAIIVAVIIAIPIIFLGLLYFYQEKLFFHPVKLGAN
                     YAFEFDQKFEEIYIQSDDGTILNGILFQSDTTKGVVFYLHGNAGSIRSCGKVAQTYTN
                     LNYDIFVLDYRGFGKSEGKIKSEEQLYSDSQIAYDELKKRYDENDIIIEGYSIGTGLA
                     SELASKNNPKLLILKAPYYSFEDLVCGTYHLPEFLLRYKIPTNEYLEKVKAPVIIFHG
                     TEDKIINYDSSLKLEAEFKEGDTLITLEDQGHGNITSNESYKTEIAKILE"
     gene            complement(2203..3603)
                     /locus_tag="PLI69_01795"
     CDS             complement(2203..3603)
                     /locus_tag="PLI69_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HOG41257.1"
                     /translation="MKPFLMFPAEFIHTLEIHFGEERTHRLLSALSAEPSLSVRANLA
                     KISTEGLAEYFYDYYDGIVPWCPEGLYLKSRPSFTLDPLFHAGYYYVQEASSMYTGVL
                     LKRAISQLSEGSPFDRFGVSPSSDRPEGSISTEGPGLRLLDLSAAPGGKSTHILSIID
                     PNDLLVSNEVIRSRASILAENIARWGYPNVVVSNNDPADFIALDDFFDIVLVDAPCSG
                     EGMFRKDPDALKEWSVDNVRLCASRQRRIVSDIWQALRSGGYFIYSTCTFNTLENDEN
                     VAWIASELGAELIEQRQFLPGEERGEGFFIALLRKNGDYYPSVKNLAIKKSQIPACAS
                     YILQDLGQFTFSLKGDLWKVIPSQLVTDIKALESSLKVIRSGVAVAVQKGRDMIPEAD
                     LALSMVLDKKAFHTVELSIEDALKFLSKENLVFGTEVPLGYLLVCFEGAPLGFVKNLG
                     RRSNNLHPISRRIRMQ"
     gene            complement(3575..4018)
                     /locus_tag="PLI69_01800"
     CDS             complement(3575..4018)
                     /locus_tag="PLI69_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41258.1"
                     /translation="MAEEICGKTVVIDRPSYSIYTAFSDLRNFVAAIPEDKKEMVTAD
                     ADTISAKVQGFEIGMRVHKRTPFSRIDFEQDGQAPFPFLFSLIMDPMDDNRTYFHIEL
                     RAELNTMMKMVIGSRLHDLVDKLTEGIAKAASGEMPDEAFFDVSR"
     gene            complement(4020..>4223)
                     /locus_tag="PLI69_01805"
     CDS             complement(4020..>4223)
                     /locus_tag="PLI69_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOG41259.1"
                     /translation="GFVEGMVAIFSYNFHRTRQAFEDANVELHILSNYDALLEEAVAS
                     GYIKAGDLKVLDEWRFKPDTWGR"
     assembly_gap    4224..4233
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4234..4660)
                     /gene="pyrE"
                     /locus_tag="PLI69_01810"
     CDS             complement(<4234..4660)
                     /gene="pyrE"
                     /locus_tag="PLI69_01810"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOG41260.1"
                     /translation="MNNIEKIVARHLLEIKAVQLRPEEPFTWASGWKSPIYCDNRKIL
                     SYPEKRTEICGYLVEVIKEHFSDVDIIAGVATGAIAYGALVAHALGLPFIYIRPKAKD
                     HGTLARIEGDLPKGANVVIIEDLISTGTSSLSAAEAVAKE"
     gene            4716..5336
                     /locus_tag="PLI69_01815"
     CDS             4716..5336
                     /locus_tag="PLI69_01815"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOG41261.1"
                     /translation="MYRIHFESRNIIVCSSASDAEEDSCALILSVGEKEHLGNIPMMF
                     EKSSRMPSLYIPTSDRERVCNELCSKYRILDAAGGVVRDPSGRILMILRNGKWDLPKG
                     KLEPGESSQEGALREVAEETGIDRLTIGNLICVTHHTYSREGERILKHTRWYEMKYEG
                     KSAPDPVPQTEENIAVAEWVPVEEIPAKLSNSYTSIREVFRQAGII"
     gene            complement(5329..5847)
                     /locus_tag="PLI69_01820"
     CDS             complement(5329..5847)
                     /locus_tag="PLI69_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41262.1"
                     /translation="MRRILLSLSILFFSCTLWGQGSGLLLDKISQIGSVTLEYTLTTN
                     ASDGRLLMEQRGTIDIQKMSYRLTSDLLIIISDGTTRWLYSPKSEELVICSADYSSED
                     PLENPFSLLASTSMEQKKDGTFKIVHKGANGYVYTVLVNSVSKKETPWDDSYFILNVD
                     SLSEDVIITDLR"
     gene            complement(5873..8314)
                     /locus_tag="PLI69_01825"
     CDS             complement(5873..8314)
                     /locus_tag="PLI69_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOG41263.1"
                     /translation="MNQSKAAKNKKTGRNKVAKESREPWLSSADARVLRILLGFIFLA
                     VTVFTVVALVSYIFTWNSDQSLVRDSQVLSITAEEASNSGGRLGLLWADFLISKLFGL
                     GAFAVPFFFGAISIASFRIKKVNLLRFFIVSLIGAIVISLILAFVSGFTKYEFFLGNG
                     PGGSYGRHAVSWLTSMLGNVGTGSALIILLLLWLVLLSKKVALRFDDLMWSVANRPRK
                     EELPVTSEGSEIETTEAVEEDTLGEDTDSDPESRELPQEATTEVIPDPDPFPAKEEES
                     LSPTVSDNPDDIIIQVEGSEHDFIKNLTEEEKSRLFDPRLDLPDYRHPSLSLLDDYRD
                     KWYTVSMEELEKNKTKIVTVLGHYKIKVVAITAKMGPTVTLYKIHLADGMKISQVRSR
                     EEDIALSLGAKGVRVVTLIDSVGIEVANENPSIVPLKSILNDPKFKNTNMELPLAMGI
                     TVTNEPFFLDLAKMPHLLIAGATGMGKSVGLNCIITSLLYTKHPAELKFVMVDPKKVE
                     LSLYAKLDKHFLAKVSDESEAIITDTRKVVNTLKSLCIEMEDRYDLLKMADLRHIRDY
                     NNKFLNRRLNPQKGHKFLPYIVVVIDEFADLLVTAGKEIEEPIARLAQQARAVGIHLV
                     IATQRPTTDIITGTIKANFNSRIAYKVNASIDSRTILDTTGANRLIGRGDMLVMYPGE
                     DIVRVQCAFVDTPEILAVTKEISAQDGYDHEFYLPEVSDEEDSSGGNDYGVNLKNRDA
                     LFEDAAKIVVMSQQGSTSLIQRKLEIGYARAGRIIDQLEAAGIVGPFGGSKARDVLVS
                     DLATLDIILETLDRV"
BASE COUNT         2449 a         1940 c         1838 g         2145 t
ORIGIN      
        1 caaacagaca aacttttcat tatgaatttc ggaacacttc taaagcggat tctattttca
       61 gcaatacttg caatactatc ctcaactctt actgcacaaa gacagggcat cttttccata
      121 gtcacatgcc cggcagaaaa cacctcaaag gctatgaaca tcagttgggg agcggatacc
      181 acaatcacag aatgtatcat agaatacact cttgcacggg atacgcagtg gaaagaatcg
      241 agaacaatgg aggaaacaag ccatctttgc accgtgtttg ataaggtcta ttccaaaaaa
      301 gccgacggta ccgactttta tgaagatgct attttcctca aatatgaagc agccctcgaa
      361 gagctgaagc cccgtagaga atacaaatac cgcattgtca gcgaacgttg taccagcgat
      421 acttataagt tcagaacatc cggcagcaac cgctggagtg cctgtatcat tgcggatttt
      481 catgtctatc cgccgttgcg cagccgtctt gacaatgcaa tggcaatgat ggataaggtg
      541 caagaatatg accccggatt tgactgggta ctccaccttg gagatatcat ttcctgggga
      601 ggcagttatt cattctggca ggagatgtat gaagaggagc attttaagaa ttatatgtgg
      661 gccggtgtaa acggcaacca cgacaatatg tcacgtggtt atattaagaa tacaaatgag
      721 tatttcaaag aggccaattt ttatccacgt aacggatata aaggagaaga gggggtctgc
      781 tactggtttt actggggaga tgctctattc ataatgctca acaacgagag catgagggaa
      841 gaagagggtc tgaacgctgc tcaggaatgg gtgagagagg tagtcaaaaa taatccagcc
      901 agataccgtg tagtatgtga acattaccag tggttctttg gtgacagtgg caaaacatcg
      961 caatacggca gatggagcga tctgtttgac gaacttggca tagacctggc tctggcaggc
     1021 aataaccata tatatgtgag caccaatgca atttatcagg ggaaagagag cgacggcaaa
     1081 aaaggcacgg tatatatcca ggcaccctcc tccgacaatg agcgtggcgt acccactccc
     1141 aatccgctta cggaaaatct tgataaaatc cgcttccgct ggagcgaagg cgcaatgact
     1201 gtcggagcca tccacctcga tgtaaataaa aagagaatgg tactcactct ccatgaccgc
     1261 tacggcaaca ttcttgacaa agcggaagta aaatcaaaaa aataaaaaag gcacttgcct
     1321 gaatagtttt aaaaaccaac acaatgaaga aaagaaagaa gcgcattata gcaataatag
     1381 tcgcggttat aattgctatc cctatcatct ttttaggact attatatttc tatcaggaaa
     1441 aattattttt ccaccccgta aagctgggag caaattatgc ctttgagttc gatcaaaaat
     1501 ttgaagaaat atatattcaa tctgatgatg gaactatttt aaatggcatt ttattccaat
     1561 ccgacacgac taaaggagtg gttttctact tgcacgggaa cgctggttca atacgttctt
     1621 gtggaaaagt cgcacaaacc tataccaact taaattacga tatcttcgta cttgattaca
     1681 gaggatttgg taaaagtgaa ggaaaaatca aaagcgagga acaactttac tccgatagcc
     1741 aaattgctta tgatgaactt aagaagcgat atgatgaaaa tgacattata atagaaggct
     1801 actcaattgg aaccggatta gcttcagagc ttgcctctaa gaataatccg aaactgctca
     1861 ttttaaaagc tccttattat agttttgagg atttggtatg tggcacctac cacttaccgg
     1921 aatttctttt gagatataaa ataccgacaa atgaatatct ggaaaaagtc aaggctccgg
     1981 tgattatttt ccacggaacg gaagataaaa taataaacta cgactcctct ctcaaattag
     2041 aggctgaatt taaagaaggc gacacactta tcacattgga ggatcaggga cacggtaata
     2101 taacatccaa tgagagttat aaaactgaaa tagcgaaaat attagaataa tgtaagatta
     2161 aaaacagaac agagctcttt gaatcctctt aacctgcctg cgctattgca ttctgattct
     2221 gcgggagatg ggatgcaggt tgttggaacg acggccgaga tttttgacaa agcccaatgg
     2281 agctccttca aaacagacca aaagatagcc cagggggact tcagtaccaa ataccagatt
     2341 ctcttttgag aggaatttca gggcatcttc aatagagagc tccacagtgt gaaatgcctt
     2401 tttatccaaa accattgaaa gggccagatc tgcctcagga atcatatcac gccccttctg
     2461 cacggcaact gctactcccg aacgaatcac tttaagagaa gactccaaag ccttaatatc
     2521 ggtcaccaat tgtgacggaa tcaccttcca tagatctcct tttagggaga atgtaaattg
     2581 ccctaaatcc tgtaatatat aggaggcaca agcaggaatc tgactctttt taattgccaa
     2641 attcttaaca gatggataat agtcaccatt tttgcgcaat agcgcaatga aaaatccctc
     2701 ccctctctct tctccaggga gaaactgacg ctgctcgata agttccgcac caagctctga
     2761 agcaatccaa gccacatttt catcattctc gagagtgttg aatgtacagg tggaataaat
     2821 gaaatatccg ccactacgga gagcttgcca tatatccgag actattcttc tctgcctgga
     2881 agcacatagg cgtacattat ccacagacca ctccttcagg gcatcgggat ctttgcggaa
     2941 catcccctcg cccgaacaag gagcatccac cagaacgata tcaaaaaagt catcaagagc
     3001 gataaaatcc gcgggatcgt tgtttgatac cacaacattg ggatatcccc atcttgcaat
     3061 attctcagcc aggattgaag ccctgctacg gattacctca tttgagacca aaagatcatt
     3121 tggatcgata atcgaaagaa tatgcgtgct cttacctccc ggagcggcag agagatccag
     3181 aagacgaagt ccgggcccct cagtcgaaat agagccttcc ggacgatcgc ttgaaggaga
     3241 aaccccaaac cgatcgaatg gtgatccctc ggacaattgt gatatcgccc gtttcagcag
     3301 cactccggta tacattgagg aagcctcctg tacatagtaa tatcctgcat gaaaaagagg
     3361 atccaatgta aatgagggtc tgcttttgag atacaatccc tccggacacc agggtacgat
     3421 gccgtcataa tagtcataaa aatactctgc aagtccttcc gtagagattt ttgcaaggtt
     3481 tgcacgaacc gatagtgaag gttctgctga aagagcagag agaaggcgat gagtacgctc
     3541 ctctccaaaa tgaatttcaa gagtatggat aaactcagcg ggaaacatca aaaaaggctt
     3601 catccggcat ttcaccgctt gcagctttgg caatgccctc tgtaagtttg tcaaccagat
     3661 cgtgtagtct gctccctata accattttca tcatagtatt gagttctgca cgtagttcaa
     3721 tatgaaaata ggtacggttg tcatccatcg gatccattat cagagaaaaa agaaatggaa
     3781 acggggcttg cccatcctgt tcgaaatcta ttctcgaaaa aggtgttctt ttatggaccc
     3841 tcattccaat ctcaaaaccc tgtacttttg ccgatatggt gtcggcatcg gcggtcacca
     3901 tctccttttt atcttccgga atggcggcca caaagttgcg aaggtcggaa aaagcggtat
     3961 atatcgaata ggaaggccta tctatgacta cggtcttgcc acaaatctct tctgccatac
     4021 tatctgcccc aggtgtccgg tttgaatctc cactcatcaa gtaccttgag atctcccgcc
     4081 tttatgtaac ccgacgcaac cgcctcttcg agaagtgcat cataattgct cagaatgtgc
     4141 agttccacat ttgcatcctc aaaggcctga cgtgtcctgt ggaaattata ggagaaaatg
     4201 gcaaccatac cctctacgaa accnnnnnnn nnncctcttt ggcaacagct tcagcagctg
     4261 agagcgaact tgtacctgta gaaataaggt cctcaataat aaccacattg gctcccttgg
     4321 gcagatctcc ctcaatacgg gccaaggtac catgatcctt agcttttggt ctgatatata
     4381 taaagggcaa tcccagggca tgcgctacca gtgcaccgta tgcaatggca cctgtggcca
     4441 caccggcgat aatatccaca tcactgaaat gctcttttat gacttctact aaataaccgc
     4501 agatttcagt acgcttttcc ggataggaaa gaattttgcg gttgtcgcaa taaatgggtg
     4561 acttccaacc tgatgcccaa gtaaaaggct cttccggcct caactgtacc gcctttattt
     4621 caagcagatg gcgggcaact attttttcaa tattattaat cataatctat ttttggctta
     4681 tctttgcaaa ggtaataata ttattcggta aaacgatgta tcgtatccac tttgaaagcc
     4741 gtaacatcat cgtctgctct tcggcctcag atgccgagga agatagctgc gctcttattc
     4801 tcagtgtcgg tgaaaaagag catctgggaa acatacccat gatgttcgaa aagtcatctc
     4861 gtatgccctc gctatatatc cctacctccg acagagagcg ggtttgtaat gaactatgct
     4921 cgaaataccg tattctggat gcagcaggag gagtagtgcg cgacccttca ggacggatat
     4981 tgatgatact tcgcaatggc aagtgggacc ttcccaaggg gaaattggag cccggagaga
     5041 gcagtcagga gggtgctctg cgagaggtag cggaggagac cggcatagac cggcttacca
     5101 taggcaatct tatatgcgta acacatcaca cctactccag agagggcgag cgtatactta
     5161 aacacacccg gtggtatgaa atgaaatatg aaggcaaatc cgcacccgat cccgtgcctc
     5221 agactgagga gaatatcgcg gttgccgagt gggtacctgt ggaagagatc cctgccaaac
     5281 tatccaacag ctatacatcc ataagagagg tattccgtca ggcaggcatt atctgagatc
     5341 agttattatt acatcctcag acaacgaatc aacattcaga atgaagtagg aatcatccca
     5401 gggagtctcc ttttttgaca ctgaattcac aagcacggta taaacatagc cgttggcacc
     5461 cttatgcact attttaaaag tgccatcctt cttctgctcc atactcgtgg atgcaagcaa
     5521 tgagaaagga ttctccagcg ggtcttcgga tgagtaatct gcactgcata ttaccagttc
     5581 ttcgctctta ggactataaa gccacctggt agtaccgtct gagatgatga taagcagatc
     5641 actggtaagg cggtaactca tcttctgtat gtcgatagtg cctcgctgct ccatcagaag
     5701 gcgtccgtca gaagcgtttg tggtcagcgt gtactcaagc gtgacactgc ctatttgcga
     5761 aattttatcc agaagcaagc cacttccctg cccccacaaa gtgcaggaaa aaaataaaat
     5821 tgacagtgaa aggagaattc gtctcatacc tttatagggt acaaacatcg tgctagacac
     5881 ggtcaagagt ctcgagaatt atatcgagag tggcaagatc cgacaccaga acatctctgg
     5941 ccttgctgcc gccgaatggc ccgactatac ccgccgcctc gagctggtcg ataatgcgtc
     6001 ccgcccttgc atagcctatc tcgagcttac gctgaataag agaggtggat ccttgttgag
     6061 acatcactac tatctttgca gcatcctcaa acagggcatc gcgatttttg agattgactc
     6121 cgtaatcgtt gcctccggag gaatcttcct catccgacac ctccggcaga taaaattcat
     6181 ggtcatatcc atcctgagca ctaatctctt ttgtaacggc aagaatctcg ggtgtatcca
     6241 caaaggcaca ttgtacgcga acgatatcct cgccgggata catcaccaac atatctccac
     6301 gaccgataag gcggttggct ccggtcgtgt cgagaatggt ccttgagtcg atggatgcat
     6361 tcaccttgta tgctatcctg gagttgaaat tagccttgat agtacctgta atgatatctg
     6421 tggtgggcct ctgagtagcg attacaagat ggattccaac agctcttgcc tgctgcgcaa
     6481 gacgggcgat gggctcttct atctctttac cggcagtaac gagcaagtcg gcaaactcat
     6541 ctatgacaac cacgatatat ggtaaaaact tgtgaccttt ttgtggattt agacgacggt
     6601 tcaggaactt attgttgtaa tccctgatat ggcgcaaatc tgccattttg agcagatcat
     6661 aacggtcttc catctctatg catagagact tgagtgtatt cactaccttt cgtgtatcag
     6721 ttatgatggc ctcggattcg tctgaaacct ttgccaggaa atgtttatcc agcttggcat
     6781 agagcgacag ttccaccttc ttgggatcta ccatgacaaa tttgagctct gccggatgtt
     6841 tggtatagag aagcgaagtg atgatacagt tgagacctac tgattttccc attccggtcg
     6901 ctccggcaat aagcaggtgc ggcatcttgg caaggtcgag gaaaaagggt tcgttggtta
     6961 cggtaatacc catcgccaac ggaagttcca tattggtatt cttgaattta gggtcgttga
     7021 gaatggactt gagcggcaca atggagggat tttcattagc cacctcaata cctaccgagt
     7081 cgataagcgt cactacacgc acaccttttg ccccgagaga tagagcgata tcctcttcac
     7141 ggctacgcac ctgcgatatc ttcataccgt ctgccagatg aatcttatag agagtaacag
     7201 tagggcccat ttttgcagtt atggccacta cttttatttt gtagtgcccc agcactgtca
     7261 caatcttggt tttgtttttc tcaagttcct ccattgagac ggtataccat ttatccctat
     7321 aatcatccaa caatgagagt gagggatggc gatagtcagg caagtcaagt ctggggtcaa
     7381 agaggcgaga tttctcctct tccgtaagat tttttataaa atcatgctcg gagccctcaa
     7441 cttgtatgat gatatcatcc ggattatcac tcacggttgg agatagggat tcttcctctt
     7501 tagcagggaa aggatcggga tcggggataa cctctgtggt agcctcctgg ggcaactcgc
     7561 gggattcggg gtcagaatca gtatcttcac caagagtatc ttcctcaaca gcctctgtcg
     7621 tctcaatttc agaaccctcg gaagtgaccg gcaattcctc cttgcgagga cgattggcca
     7681 cactccacat aaggtcatca aaacgcaacg ccaccttctt gctgagcaaa accagccata
     7741 gaagcaagag tataatcagc gcactgccgg ttcctacgtt gccaagcata gatgtaagcc
     7801 aggaaacggc atgtctgcca taagatccgc ccggaccatt gccgagaaag aactcatatt
     7861 tggtaaaacc ggaaacaaat gcgagaataa gcgaaatcac aatggctccg ataagcgaga
     7921 ctatgaagaa acgcaacaaa tttacttttt tgattctgaa agaggctatc gaaatcgccc
     7981 caaaaaagaa gggcactgca aaagctccca gaccaaataa ctttgaaatc aggaaatccg
     8041 cccaaagcag cccaagtctg ccacccgaat ttgaagcctc ttcggcagta atggaaagca
     8101 cttgtgaatc ccgcaccagg ctctgatcgg agttccatgt aaatatatat gagacaagcg
     8161 ccacaacggt aaaaacagtc accgccagaa aaataaaccc gagcaatatc ctcaacacac
     8221 gggcatccgc actggaaagc cagggctcac gggactcttt tgcgacttta ttcctacccg
     8281 tttttttatt tttggctgct tttgactggt tcattgctca cggagatcag tttttctttt
     8341 tctttttgtt cctggatttt ttgctgaatc cggatttgga cg
//
LOCUS       DAOSIE010000030         8232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_8111_length_8232_cov_3.801394, whole genome shotgun sequence.
ACCESSION   DAOSIE010000030 DAOSIE010000000
VERSION     DAOSIE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8232
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8111_length_8232_cov_3.801394"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="PLI69_01830"
     CDS             <1..134
                     /locus_tag="PLI69_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG41264.1"
                     /translation="AAQMMGVPSRMILFPDENHWILKPQNSIHWNREFFTWLDTYLK"
     gene            182..1573
                     /locus_tag="PLI69_01835"
     CDS             182..1573
                     /locus_tag="PLI69_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOG41265.1"
                     /translation="MFDKKIYISRRKRLVESISEGIILILGNSEASFNYASNTYRFRQ
                     DSSFLYFFGLDKPDLAAVIDVQSGEEIIFGNDVDIDDIIWMGPQPLISEQAASVGVVK
                     SAPFASLATYLSEAKAKGRKIHFLPPYRNHNKILLHQMLGIGFDKMKEAAGVELIKAV
                     VALREIKEECEIAEIERACDLGYDMHMAAMKAMKLGMVEQELVGIMEGICISGGVMPS
                     FPIILSQNGETLHNHMHHQILTEGRLCVIDAGAEIDTHYSSDFTRTLPSGGNFTKQQA
                     EIYSLVAAANNYAIGASRPGISYTDVHLGAVTVLVQGLIDLGLVNGNASEAVAAGVAG
                     LFMPHGLGHQMGLDVHDMEDLGENYVGYDDKYHRSTQPGLASLRMGKMLKVGHVVTVE
                     PGIYFIPALIEKWQNEGICKGLINFSKLKPYYKFGGIRLEDDILITSGGCHLLGAKRL
                     PITVEDVEREMTT"
     gene            1586..2059
                     /locus_tag="PLI69_01840"
     CDS             1586..2059
                     /locus_tag="PLI69_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOG41266.1"
                     /translation="MLAIKIIAVMLGIAGLVGCILPVIPGPPFSWVGLLLLYFWGDEP
                     MSLRFLLIWLVITIVVTILDYLVPAYFTKVTGGSRAASRGSLVGLLVGLIFFPPIGMI
                     VGAFVGALLAELLINGSRMAHSFKAAMGAFIGFFFGTFLKIATSAVMMFYIVKFL"
     gene            2343..5720
                     /locus_tag="PLI69_01845"
     CDS             2343..5720
                     /locus_tag="PLI69_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOG41267.1"
                     /translation="MWAQNITVTGKVADSKGEPIVGAYVLVEGTRQGTSTDFDGVYSL
                     PAPRNGALVFSCMGYRDLTVPVNGRSTIDVVLQEDAMLLEDAVVVGFGTQRRENLTGA
                     VAAVNVSQALESRPIADVGRGLQGMTPGLTVRVGSTEVGSDPLFRIRGHVGSYLGGSS
                     PLILLDNVEIPSINLVNPDDIESISVLKDAASASIYGAKAAFGVILITSKKGAKTETI
                     KVNYSANFSFQSLTDTDGIADVDALHYTVEAAERLKTFTPVGAFWLVDRAGYNAAVAW
                     KEKYGKTLDPYAPMTYGRDWYIDSSNRKIGVRTYDPYVYMINQGAPTQSHNLSVQGKT
                     GKTNFNISLGYLGQSGMLKPTEYDDFTRYNANVRLSTQVNDFINVHAGMMYTNSNKSW
                     AFSTNSTTADPWYYLFRWGPTYPLVPTDEFGNSLRNQVYEMGTANEANIKTNYTSVNV
                     GATVTPIKNWDINFDYTYAVNNSQEFNPGITYWAANLWSGAQAVKDAQGNFVNVSNEW
                     DQFNKLGPSIIQQTLPVQSYTSSYDSYYQDSYTSIRQTWNITTDYALKLANHKFDFLL
                     GMQAVDYRATGVWGKKMTLMDIKNPQFALATGTETTGGSNSWSSTLGFFGRVNYNYKE
                     KYLFEANLRYDGSSKFPTHLKWRWFPSFSAGWRLTQEPWMQDITHVLSSLKLRASWGM
                     IGDQTVSSSQYIPLIGAQTTYWIHGGVKDNAYATPSLVASDITWQDIVTLDFGLEFSL
                     FNQVDFVLGWYQRDTKNMIVGMEGLGYNLGSPPPNGNFGSLQTRGWEATVNYRKIFDN
                     GFSLSASASISDAKTIITEYGSALGIHGWYNGKTYGEIWGYRVDDLYTWDDFGLQPGQ
                     WKQLEQLIATDKYGRYYQNVKGYASQGKITSGSAISGPGDVRFKDLNGDGMIDNGSQL
                     LTIKDADGNDVPGYGDLDVIGNTTPRYEYSFRVDLGWKGFDFSMFWQGIGQRSLWGGS
                     ALTIPGFNSSDGAMAQAIAGNFWYETLDASGNVVDARYDAFYPRAHNLYSSGTGFNMQ
                     VNDRYLLNMAYLRLKNVTLGYTIPEKITKKAFIDKLRVYVSFENFLTFDKLRGLPIDV
                     EEIPGYSYLNSSNYNSSRTGVGAPAFRSASFGVQLTF"
     gene            5736..7805
                     /locus_tag="PLI69_01850"
     CDS             5736..7805
                     /locus_tag="PLI69_01850"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG41268.1"
                     /translation="MKKILYILMAFALLVPTSCSDFLERSSLVDLDDNTYWSSEGNVR
                     LFVNGAYAYYFSGYSDNWGSVYAPEVYATPYNDDRTLTGEQPNILLSVPADNWYRNES
                     TAYRGTWLSRRGSAPWNFAYVRKWNLLMERLDMMKEAGNLTEEGYNHWMGVARFLRGW
                     EYSRMVQSFGDVPYYDKQVESADFDAQFKDRDPRTYVMDQVKADFDYAMANVRVNDGV
                     DYINRYVVSTVAARCMLFEGSWLTYHKNDAALKTCSDIDGHAKSYLQAAVDYAAVTIN
                     SGKYGFNKDFRTLFGTLFTTLTGTANEVLLYRVYNKSINNSSQHCYASYSGGNNAREA
                     QRTSGNFATLKAWICQDGRPYNSSTVPNVSSFRMQDMVVTRDPRFEASFLDETWTTPT
                     GLYVFKFIDREGCDYFWKGGTSPAYYNSCNNENGFPCIRYAETVLNWLEAKAILADFF
                     GGPAVTQADLDKSINAIRTRPLDQVAISKGVKKTAPLTLAMVSSMNDPERTSNIQKTT
                     LGYNTRGFCSPLMWEIRRERRMEFFMESVRTLDIRRWGWLELMLSTNNPDIGVGGWVE
                     LDLARNKPADNPTQITNLKRTKNGVGYNLLTAPNINVVSVRHIEKVNADGSIVLGPRI
                     FFNGSNYSQMRGFLVPQNFKDFDPRNVDVRNYLEPICTDIINQYKEKSMATGNNYTIY
                     QNPGWEQ"
     gene            complement(8049..>8232)
                     /locus_tag="PLI69_01855"
     CDS             complement(8049..>8232)
                     /locus_tag="PLI69_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOG41269.1"
                     /translation="TTEKDAQRLVSNKHLTPELKKRLFYIPIETEFLTDEESDGFYDF
                     ITEVLPSENVPNGMLF"
BASE COUNT         2086 a         1969 c         1927 g         2250 t
ORIGIN      
        1 atgccgccca gatgatggga gttccctcga ggatgatttt gttccccgat gaaaatcact
       61 ggatacttaa acctcagaat tccatccact ggaaccgcga atttttcact tggctcgaca
      121 cgtacctgaa atagttctga gtattgagtt acaaataata acatattaat acaaataaac
      181 aatgtttgat aagaagatat acatttcaag gcgcaagaga cttgtggaaa gcatttcaga
      241 gggaataatt ctcattctcg gcaatagcga ggcgagcttc aattatgcaa gcaataccta
      301 tcgattcagg caggatagtt ctttcctcta tttcttcggt ctggacaagc ccgatctggc
      361 tgccgtcatt gatgttcaga gtggagagga gatcattttc ggcaatgacg tggatataga
      421 cgacatcatc tggatgggtc ctcagccgct tatttcggaa caagcagctt ctgtaggcgt
      481 ggtcaaaagc gcacctttcg cctctttggc aacttatctg agcgaagcta aagccaaagg
      541 acgtaaaata catttcctgc ctccttaccg caaccacaat aagattcttc tccaccaaat
      601 gctcgggatc ggatttgaca agatgaaaga ggctgccggc gttgagttga ttaaggcggt
      661 ggtagctctt cgtgagatta aagaggagtg cgagattgct gagatagaga gagcttgtga
      721 tttgggctat gatatgcaca tggctgcgat gaaggcaatg aaattgggaa tggttgagca
      781 ggaactggtt ggaataatgg agggaatttg catctccggc ggcgtcatgc cctcttttcc
      841 gattattctt tcccaaaatg gcgagactct ccacaaccac atgcatcatc agatcctgac
      901 cgaaggaaga ctatgcgtaa ttgatgccgg agccgagatt gacactcatt attcatcgga
      961 ttttacccgt acgctccctt ccggcggcaa ttttacgaag cagcaggctg aaatatattc
     1021 actggttgct gccgcaaata actatgccat tggagcttca cgtccgggta tctcttatac
     1081 cgatgttcat ctgggtgcgg tgacggtgct ggtgcagggt ctgatagatt tgggccttgt
     1141 caatggaaat gcatccgagg cggttgcggc aggtgtggcc ggtctcttca tgccccatgg
     1201 tttggggcac caaatgggcc tagacgtgca tgatatggaa gatttgggtg aaaattatgt
     1261 cggctatgat gacaaatacc atcgctcgac ccagcccggg ctggcttccc tgaggatggg
     1321 taagatgctc aaggtcggac atgtagtgac cgttgaaccg ggaatctatt ttattcccgc
     1381 tttgattgag aaatggcaaa atgagggcat ttgcaaaggt ttgatcaact tttcaaaatt
     1441 gaaaccctat tataaattcg gaggtatccg tctggaagat gacatattga tcacgtccgg
     1501 aggatgtcat ctgctcggag caaaacgtct tccgataact gtggaagatg tagagcgtga
     1561 gatgaccacc tgatttggag caattatgtt ggcaatcaaa ataatagcgg ttatgctcgg
     1621 gattgccgga ctggtgggat gcattctgcc ggtaatcccg gggccgccct tcagctgggt
     1681 ggggctcttg ctactttact tttggggtga tgagccaatg tccctgcgct ttctccttat
     1741 ttggctggtc atcaccatag tcgtgaccat tctcgactat ctggtgcccg catatttcac
     1801 caaggtaacg ggcggctcca gggccgcctc ccgtggttct cttgtggggc ttctagtggg
     1861 attgatcttc tttcccccta taggaatgat tgtgggagcc tttgtcggcg cattgttagc
     1921 ggagctgttg atcaacggta gcaggatggc acactcgttc aaagctgcca tgggcgcttt
     1981 tataggcttt tttttcggta cctttctcaa aattgccact tccgcagtaa tgatgttcta
     2041 tattgtgaaa ttcctttaaa atgagcctat tagttgtcag ttttggactc agaaataaaa
     2101 ttgacgacac ttttttaata ataaagggta atttttgaaa aataatgtta aatttgcgga
     2161 tgcatatata tattggcttt tggctcatat ttgtataata accgaaagaa attactaaag
     2221 caaattgtgt aaaaacttaa aattaaactt tttattaacc taaaatttca tttatgttcg
     2281 tttctaataa actaggccgc atctttgcgg ccgtgtgtgc attgctccta tcaatgggaa
     2341 caatgtgggc tcagaacatt actgttaccg gtaaggttgc cgacagtaag ggagagccga
     2401 ttgttggcgc gtacgtactc gtagagggta cacgtcaggg aacatctacc gattttgatg
     2461 gtgtctattc gcttccggct cccaggaacg gcgctcttgt cttttcctgc atgggttata
     2521 gggatcttac cgttcccgta aacggcagaa gcactattga tgtggttctt caggaagacg
     2581 caatgcttct tgaggatgcc gttgttgtag gtttcggtac gcaaagaagg gagaacttga
     2641 caggtgcggt tgcagctgtg aatgtttcac aggctcttga aagccgtcct atcgccgacg
     2701 taggccgtgg tctccagggt atgactcccg gtttgacagt acgcgtgggt tctaccgagg
     2761 ttggttccga tcctctattc cgtattcgtg gccacgtagg ttcatacctt ggtggctcat
     2821 ctcctctgat ccttctggat aacgtggaga ttccttccat caacctcgtc aaccctgatg
     2881 acatcgagtc catctctgtg ttgaaggatg ccgcctcagc ctctatctac ggtgcgaaag
     2941 ctgcattcgg tgtaatcctc attacttcca agaagggtgc gaagaccgag actatcaagg
     3001 tcaactactc agcaaacttc tcattccaga gtcttaccga cactgatggt attgccgatg
     3061 ttgatgcact tcactacacg gtagaggcag ctgagcgtct caaaaccttt actcccgtag
     3121 gtgctttctg gcttgttgac cgtgcaggtt acaacgccgc agtagcatgg aaagagaaat
     3181 atggcaagac cctcgatcct tatgctccca tgacctatgg tcgtgactgg tatatcgact
     3241 cttccaatcg caagatcggt gtacgtactt acgatcctta tgtttacatg atcaaccagg
     3301 gcgcccccac gcagagccat aacctctccg ttcagggcaa gaccggtaag actaatttca
     3361 atataagcct cggttatcta ggccaaagcg gtatgttgaa gccgactgag tatgacgact
     3421 tcacccgtta caatgccaac gtgagacttt caacccaggt taatgacttc atcaacgtac
     3481 atgctggtat gatgtacacc aattccaaca agtcctgggc gttctctacc aactccacta
     3541 cggctgaccc ctggtattat ctcttccgct ggggacctac ctatcccttg gttcctaccg
     3601 acgagtttgg caactctctc cgtaaccagg tttatgagat gggtactgca aatgaggcca
     3661 acatcaagac caactacaca agtgtaaacg ttggtgccac cgtaaccccc attaagaact
     3721 gggatattaa ttttgactac acttatgcag taaacaactc ccaggagttc aatcccggta
     3781 ttacttattg ggcagccaac ctttggagtg gagcacaagc agtaaaagat gcccaaggca
     3841 attttgttaa cgtttcaaac gaatgggatc agttcaacaa actgggcccc tctattattc
     3901 aacagactct tccggtacag agctatactt ccagttatga ctcttactat caggattcat
     3961 atacctctat cagacagacc tggaatataa ctaccgacta cgcgcttaaa cttgctaatc
     4021 ataagtttga tttccttctc ggtatgcagg cagttgatta cagagcaaca ggcgtatggg
     4081 gtaaaaagat gaccttgatg gatattaaaa atccccagtt tgcacttgca accggcacgg
     4141 agactacagg tggttccaac tcatggagct ctactctcgg attcttcggt cgtgtaaact
     4201 acaactacaa ggagaaatat ctttttgagg cgaaccttcg ttatgacggt tcttcaaaat
     4261 tccccactca tctgaagtgg agatggttcc cttcattctc cgcaggctgg cgtttgaccc
     4321 aggagccctg gatgcaggac atcactcatg tgctctcctc actgaaactc cgtgcatcat
     4381 ggggtatgat tggagaccag accgtatcca gttcacagta tattcctctg attggcgcac
     4441 agaccacata ttggatccat ggcggtgtta aagacaatgc ttatgcaact ccgagtcttg
     4501 tagcttcaga cattacctgg caggatattg ttactctgga cttcggtctt gagttttcac
     4561 tcttcaacca ggttgacttt gtactcggtt ggtatcagag agataccaag aatatgattg
     4621 tgggaatgga gggtctcggc tacaacctcg gttctcctcc ccccaacggc aacttcggtt
     4681 ctctccaaac cagaggatgg gaagcaacag taaattatcg caagatattt gacaatggtt
     4741 tcagcctatc agcttccgca tccatatctg atgcaaagac catcatcaca gagtacggct
     4801 cggcactcgg catccatgga tggtacaacg gcaagaccta cggcgaaatc tggggttacc
     4861 gtgttgacga tctctacacc tgggatgact tcggtctcca acccggtcag tggaaacagc
     4921 tcgaacaact aattgccaca gacaaatacg gcaggtacta ccagaatgtc aaaggttatg
     4981 cctcacaggg taagattact tcgggaagtg ctatttcagg ccccggcgac gtaaggttca
     5041 aagacctcaa cggtgacggt atgatagaca atggctcgca acttctcact atcaaagatg
     5101 ccgacggcaa tgacgtaccc ggctatggtg acctcgatgt tataggtaac accactcctc
     5161 gttacgaata tagtttccgt gttgacctcg gctggaaagg gtttgacttc tcgatgttct
     5221 ggcagggtat tggccaacgc agtttgtggg gcggttcagc tcttacgata cccggcttta
     5281 actcttcaga cggtgcaatg gcacaagcaa ttgccggcaa cttttggtac gagactcttg
     5341 atgcaagcgg caacgtggtg gatgcaagat atgatgcttt ctatcctcgt gcccataacc
     5401 tctattcgag tggaacagga tttaacatgc aggttaatga caggtatctc ctcaatatgg
     5461 catacctcag gctcaaaaac gttaccctcg gttacaccat tcccgagaag attaccaaga
     5521 aggctttcat cgacaagctc cgcgtctatg tttcgttcga gaacttccta acctttgata
     5581 aactcagagg tcttccgatc gacgttgaag agattccggg ttactcatac ctcaactcat
     5641 ccaactacaa ctcaagtcgt acgggtgttg gtgctcccgc atttaggagt gcttcatttg
     5701 gtgttcagtt aacattctaa ttattgcaaa agacaatgaa aaagatatta tatattttga
     5761 tggctttcgc cctgcttgtc ccgacaagtt gtagcgattt ccttgagcgt agttctttgg
     5821 tggatttgga tgacaatacc tactggagtt cggaaggtaa tgtccgccta ttcgtcaacg
     5881 gagcatacgc ctactatttc agcggctatt ctgacaactg gggttcggtg tatgctcctg
     5941 aggtgtatgc cacaccttat aatgacgacc gcacccttac cggagaacag cccaacattc
     6001 ttctctccgt ccctgcagac aactggtatc gtaacgagtc aacggcatat cgcggtacat
     6061 ggctttcacg tcgtggatct gctccctgga actttgctta tgtacgcaaa tggaaccttc
     6121 ttatggagcg tctcgacatg atgaaagagg cgggtaattt gactgaagag ggttacaacc
     6181 actggatggg tgtagcccgt ttcctccgcg gatgggaata tagccgtatg gtacaatctt
     6241 tcggtgacgt tccctactat gataaacagg ttgaatcagc tgattttgac gcccagttca
     6301 aggatcgtga tcctcgtacc tatgtaatgg atcaggtaaa ggccgacttt gactacgcta
     6361 tggccaatgt tcgtgtaaat gacggcgttg actatatcaa cagatatgtt gtttctaccg
     6421 tagcggcgcg ctgtatgcta ttcgaaggct catggcttac ttatcacaag aatgatgcgg
     6481 ctctaaagac ctgctcggat attgacggtc atgccaagag ctatctccag gcagctgtgg
     6541 actatgcagc agttactatc aattcaggca agtatggctt taataaagac ttccgtacac
     6601 tctttggaac cctcttcacc acccttaccg gtacggcaaa cgaggttctt ctataccgtg
     6661 tttacaacaa gtccatcaac aacagctcac agcactgcta cgcttcatac tcaggtggaa
     6721 acaatgcacg tgaagcccag cgtacatcag gtaacttcgc taccctgaag gcatggattt
     6781 gccaggatgg tcgtccctac aattcttcaa ccgttcccaa tgtaagcagc ttccgtatgc
     6841 aggatatggt ggttacccgt gacccccgtt ttgaggcttc attcctcgac gaaacatgga
     6901 caacacctac aggcctctac gttttcaagt tcatcgaccg tgaaggttgt gactacttct
     6961 ggaaaggtgg tacttctcct gcatactata actcttgtaa caacgaaaac ggtttccctt
     7021 gcatccgtta tgcagagacc gttctcaact ggcttgaggc aaaagctatc cttgctgatt
     7081 tcttcggtgg tcccgccgtt acacaggcag acctcgacaa gtctatcaat gcaattcgca
     7141 cccgtcctct cgaccaggtt gcaatatcta agggtgttaa gaagacagct ccccttactc
     7201 ttgcaatggt tagctcgatg aacgaccctg agagaacttc caatattcag aagacaaccc
     7261 tcggttataa tacacgcggt ttttgttctc ctcttatgtg ggagatccgt cgtgagcgtc
     7321 gtatggagtt cttcatggag agtgttcgta ccctcgatat tcgtcgctgg ggatggcttg
     7381 agttgatgct ttccaccaac aaccccgata tcggagtagg tggctgggtt gaacttgacc
     7441 tcgcccgtaa caaacctgcg gacaatccta cacagatcac caacttaaag aggacgaaaa
     7501 acggagttgg ttacaacctt cttacagctc ccaacataaa cgtggtttct gtaaggcaca
     7561 ttgagaaggt gaatgccgac ggttcaatcg ttcttggtcc cagaattttc ttcaacggct
     7621 ctaactatag tcagatgcgc ggcttcctcg ttcctcagaa cttcaaggat tttgatccac
     7681 gcaacgtaga tgtgcgcaac taccttgagc ctatttgtac cgacattatc aaccagtaca
     7741 aagagaagtc gatggctacc ggcaacaact acacaatcta ccagaacccc ggttgggagc
     7801 agtagagatg ttgcctgacg gcaaacttaa aagagggtga cttttttggt caccctcttt
     7861 tattagtttc gagtttcaag ttccgagtat gaagtattga ttagtgcttt gcaccccaca
     7921 ctcgccccgg gccctctaac atccaccccg cactcagtga ctcgcacccc gggtcctcta
     7981 acacgcacca ctaccgacta caactatata ctacagactc aaaacccgta tccagcacct
     8041 cgcaacccct aaaacagcat gccgttggga acattttcag agggtagcac ttcggttata
     8101 aaatcgtaaa aaccgtcact ctcttcgtcg gtaaggaatt cagtctctat gggaatatag
     8161 aaaaggcgct ttttgagttc gggggttaga tgtttgttgg atacaagtct ttgggcgtcc
     8221 ttttccgtgg tt
//
LOCUS       DAOSIE010000031         8228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_8117_length_8228_cov_3.683592, whole genome shotgun sequence.
ACCESSION   DAOSIE010000031 DAOSIE010000000
VERSION     DAOSIE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8117_length_8228_cov_3.683592"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..924
                     /locus_tag="PLI69_01860"
     CDS             100..924
                     /locus_tag="PLI69_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41270.1"
                     /translation="MRKSLTGLLALLFCSCSILEDRTPCPSLLTMDYSKVLKYDFIHR
                     MSDGEYNVTLNGADFSLSESIPVHLMDTISRKSVSKGNISIFGVLSRKGKEAMIVTEG
                     NQADSLYAFNATIEATGEKTYILMDAHKQFTTVHIKESGVKNVESVAQQRLDKIQMRV
                     VGNYQGIDQNTLEPLPGKFDCIVAAASPIGEYSVRIPRQGDASIMIELTLDVSSKDTT
                     ASAARLTLPLGRYMEELGYDFSAESLADVYIGLEIDSRKAFIQIENWNFEKTFVIF"
     gene            complement(943..2226)
                     /locus_tag="PLI69_01865"
     CDS             complement(943..2226)
                     /locus_tag="PLI69_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOG41271.1"
                     /translation="MKYTKLEYGLIGEKLGHSLSGEIHRRISPRPYELCEVPKDELEL
                     FFRESNFKGINVTFPYKSAVLPFINVLSPAAASVGSVNTILRKEDGLLYGHNTDYPGF
                     NSIAKHADVVFACKNILIFGNGGTAKTLRTVALAAGAKSVMLAVRRVTGEGQVLISDL
                     ESNPQLRNDIHIIVNATPVGMSPDDEGIIVDPAIFPNLEAALDSIYNPLRTNLVLNAR
                     RLGLKAEGGLYMLIVQACFAHELFQDSVNEPISDFKKIESLYSELLHAKENIVLTGMP
                     SSGKSTIGALLAEKLGRELLDVDKIIEEQTGIEIGNTFREKGEEGFRELEKSVVASVS
                     LRQGVVISTGGGTVLDPLNVHRLKRNGRIYLLDRDLNLLIPSPDRPLASDKRQLVDLF
                     AKRRNFYLDAADAIIDNNSSLEDAVERLIEAHFRL"
     gene            complement(2241..3236)
                     /locus_tag="PLI69_01870"
     CDS             complement(2241..3236)
                     /locus_tag="PLI69_01870"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOG41272.1"
                     /translation="MKLTFGFRATVCEIIEGKGSIAHVGNLVGKARNVLVITDSGVPL
                     QYAESVLAQFYNASLLIIPQGEESKNFNTLELIIDTLRKREIDRYGALIAVGGGMTCD
                     ITGFAASCYNRGIDYYNVPTTLLAQVDASIGGKTALNFGGIKNLVGAFHHPKGVVIDT
                     DTLKTLDKREFYSGLAEVIKMAATSDAALFSLLESDLPLNEILDDIVGAALQIKCDVV
                     LEDPLEHGLRKVLNFGHTIGHAIEIASNGRLLHGESIAAGMTYFCSDEMRKRLERLLK
                     RYYLPVKSPFSGEVLLPYIALDKKRTEGLITTVFVESPGIFDFRKMTPEEVVALI"
     gene            3355..6663
                     /locus_tag="PLI69_01875"
     CDS             3355..6663
                     /locus_tag="PLI69_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG41273.1"
                     /translation="MENKFLYSVARHFYKKQENRINKLCFVFPNRRSSIFFKKYLTQC
                     MAGEALFAPKMITINDFFLELSGGQLVDKIEALYILYQQYAKVFSDEEGIAKSTFDEF
                     VYWGDVFLNDFDDIDKYLVDAEKLFTNIGDLQKLTAEENDYMSDGQKRAIKEFYTSWN
                     HSKKRDMVSKKKEEDNGEKEESGDDAENEEQKENGERENNEINKGNGERDKIDIKEEF
                     STIWENLHRLYTSFREALKDKGLMYEGMIYREVAEALKEEIKEPQEKERKEEILKKCR
                     KYDKIIFVGLNALNECEKSLFRHLNKPGSGIDVDFIWDYYGDILTDEANKSSQFMRRG
                     DKGNVVEFPQKESIEPYGTPKNPTHNATFGPGKIEIIGIPSAAGQTRYVTEILEGIHC
                     NKDGTLLPNLDLEETAIVLPDESLLHPMLNSIPKCVDKVNVTMGYPLINSHTATFVSQ
                     LEELHKKTRPNKSGGVSFHHKSVMALLSHPFLTSLLPAEGLKSIKNEIIGRNLVYVNL
                     ETLVEEQLERDDNQVDTNEQPFKGDKQQPYFGNQPIEAGEQQLSKSELFKAIFSTTPD
                     PENVGNWLEKILQLIQENLDGIDREAAYHYLRVIKRISDLNLPLTDIKTYFRLLKQIV
                     SVLSVPYRGEPLQGIQIMGPLETRALDFKNVIILSNNEGIFPSANVSNSFIPYNLRLG
                     FGLPTYEYQDSISAYHFYRSICRAEHIWLIYDSRTEGTKVGEPSRYIHQLKYGYNIPR
                     DGRRRLDIKQLAISYEMKGRKVNSLDVEKSPAIMKKLREKYIMRPDGDFNGSFSASTL
                     NDYFKCQRLFYLKHVEGIGEEDEVVEEVDGAQFGTIFHYVMEKLYLPDEKFCGVKWNK
                     SQIEGLKKDKTKIEKLILEGFAKAREKRGYSYANNTVSYDDLSGQERIVFELIADFVK
                     KTLEIDEEYAPFTFKDAEMKINYQMDIDNIGTVKIKGSIDRVDEKSGTIRIIDYKTGS
                     VSGKMRDEKGAKPNDTIPKLFKEEKNGSCVKNSVSFQLLLYKMLYLHQPDLGNGDNIL
                     PAVIALRHFHENKGVRSQEGLTKEIMDEFEEKVKEKIKEIFNEEVPFKAITFDDGEYG
                     ACRYCNYQNICKIL"
     gene            6676..>8228
                     /locus_tag="PLI69_01880"
     CDS             6676..>8228
                     /locus_tag="PLI69_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG41274.1"
                     /translation="MSDTKANPEIIIASAGAGKTHKLTETYLEMLENSYDAGKPHPHR
                     NILAVTFTNKATDEMKERILEKLFFKAANGDHNSEKLLKEILHDYSGFNVSTIDRFFQ
                     FVMKTFARELGEYGSYDVELDSEGATIEAIDRMMDSLGDDKDTTLLKWLVDYSRSIID
                     DGRSWDVRKELYKFSDLFYKEDFLIKKRGEAGYLYRLDDGSGIDPKTEIKRLLQEINR
                     ITTKFESEAKVLAGVIIAEYDRANDSVIEMKSNPRKGFVNIQNGKHTNSSDELYIKVY
                     DNSEEWCKSLKTKTGLLKVDDYKSNYAPDLQTKVNELEKHYDDNYITYNTAVTIRKNI
                     YLLGLTTDIFKELDKYLKENHLVLLSSTADILNRIIDRSDTPFIYERIGNRIDHILLD
                     EFQDTSRLQWQNFEPLIDNNIANSNDNLIVGDVKQSIYRFRGSDSSLLRDLPKNKNVT
                     PEIKYENWRSADTIVRFNSDFFETITKNDPIVKDIYTGCSQVMPSKSKNQGNLNRYDA
                     GAVKVRVFEV"
BASE COUNT         2634 a         1727 c         1781 g         2086 t
ORIGIN      
        1 gtggttgccc catgggaaga gggtggagag gttaccgaag aaatataata atgacaagtc
       61 agagattgtt gagttaacac ctttattcat gttattttaa tgagaaagag cctaaccgga
      121 ttattggcac tcctgttttg cagttgctcc attcttgaag atcggacacc atgtccctct
      181 cttcttacga tggactattc aaaagtgctc aaatatgatt tcatccaccg aatgagtgat
      241 ggagaataca acgttaccct caacggagcc gattttagct taagcgaaag cattccggtc
      301 catttgatgg atactattag tcgtaagagc gtctccaagg gaaacattag tatattcggt
      361 gttctttcga gaaaagggaa agaggccatg attgtcacag aaggcaatca ggccgactcc
      421 ctttacgcat ttaacgccac aatcgaagcc acaggtgaaa aaacctatat tctgatggat
      481 gcgcacaaac aatttacaac cgtccatata aaagagagtg gagtaaagaa tgttgaaagt
      541 gttgcacagc agagactgga taagatacaa atgagggtag taggaaacta tcagggtata
      601 gatcaaaaca ctttggagcc tctacccggc aagtttgact gcatcgtagc ggcagcctcc
      661 cctatcggag agtattcagt acgcattccc agacagggtg acgcctccat aatgatagag
      721 cttactctcg acgtatcatc aaaggataca actgcttccg ctgcaagact aacactacct
      781 ctgggacgct atatggaaga attaggctat gacttctctg ctgaaagcct cgccgacgta
      841 tatataggat tggagattga ttctcgcaaa gcctttattc aaatagaaaa ctggaatttt
      901 gaaaagactt tcgtaatttt ctgacaagca ggaaaggaga ttctataacc gaaagtgcgc
      961 ttcgattagc ctctccaccg catcttcaag agaactgttg ttgtcgataa tggcatcggc
     1021 tgcatcaaga taaaaatttc tgcgtttcgc gaaaagatca accagttgcc tcttgtccga
     1081 tgcaagtgga cgatccggag agggtatgag cagattcaga tctctatcca atagatagat
     1141 gcgtccattt cgctttagac gatggacatt gagaggatca agcactgtac ctccgccggt
     1201 agatatgaca actccctgtc gcaatgaaac gctcgccacg acactcttct caagctcacg
     1261 gaagccctct tctccttttt cacggaatgt attaccgatc tctattccgg tctgctcctc
     1321 tattatcttg tcaacgtcaa gtaattccct tccaagtttc tcggcaagca gggctcctat
     1381 tgtactcttg ccactcgatg gcattccggt taggacaatg ttctccttag catgaagtag
     1441 ttcggagtaa agcgattcta tttttttaaa gtcggatata ggttcgttga cactgtcttg
     1501 aaatagttca tgagcaaagc aagcctgcac gatgagcatg tagagtcctc cttccgcctt
     1561 cagcccaagt cttcgtgcat taagcacaag attggtgcgc agcggattat atattgagtc
     1621 caatgctgcc tccaaattgg ggaatattgc cggatcaact attataccct catcatcagg
     1681 gctcatgcct acgggagttg cgttaactat tatatgaata tcattacgca actgcggatt
     1741 cgattccaga tccgatataa gcacctgacc ctcccccgtc actctcctta ctgcaagcat
     1801 gacggattta gcacccgcag ccagtgctac ggtgcgaaga gtttttgcag tacccccgtt
     1861 accgaaaata agaatatttt tacaggcaaa gactacgtca gcatgttttg caatggagtt
     1921 gaatcccgga taatcggtat tatgaccata taggaggcca tcttccttcc taagtatggt
     1981 attgaccgaa cctacgctcg ccgcagcagg agagaggacg ttaataaatg gtaagaccgc
     2041 actcttataa gggaatgtaa catttatacc tttgaaattc gattcccgaa aaaacagttc
     2101 caactcatcc ttgggaacct cgcacaactc gtaaggacgg ggtgaaattc ttcgatgtat
     2161 ttctccggac aaactatgcc ccagtttttc tccgataagt ccatattcaa gcttagtgta
     2221 tttcataata tttttatatt ttaaattagt gctacaacct cctcaggagt catttttcta
     2281 aaatcaaaaa taccggggga ttccacaaaa acagtagtga tcaaaccctc ggtacgtttc
     2341 ttatccagag caatatatgg tagtagcacc tcaccggaaa aaggtgattt gacaggtaaa
     2401 taatatctct ttagtagcct ttcaagcctc ttacgcatct catccgaaca aaaataggtc
     2461 attcccgcgg caatggattc tccatgtaag agcctgccgt ttgaggcaat ttcgatggca
     2521 tgtcctatgg tatgcccaaa gttgagcact ttacgaagac catgctccaa aggatcttcc
     2581 agcactacgt cacactttat ttgcagcgca gcaccaacaa tatcatccag tatctcattt
     2641 aggggcaaat cagactccag caaggaaaag agagcggcat ctgaagtggc tgccatcttg
     2701 atgacttctg caagtccgct ataaaactct cttttatcca gggtcttaag ggtatctgtg
     2761 tcaataacta cgcctttagg gtggtggaaa gccccaacca gattcttgat gccaccgaaa
     2821 ttgagggcag tcttaccccc aatagaggca tcaacctgag caagcagcgt tgtgggtacg
     2881 ttgtaataat ctatgccgcg gttgtaacag gaagctgcaa agccggttat atcacacgtc
     2941 attcctcctc ctactgctat cagcgcacca tagcggtcga tctcccgttt tctaagagta
     3001 tctataatca attccaaagt attgaaattt ttactctctt ccccttgcgg tattatcaga
     3061 agggaagcat tgtagaattg agccagcacc gattcggcat attgcagtgg cacgccacta
     3121 tcagtaatca caagcacatt gcgcgctttt ccgacaagat ttccgacgtg agctatggag
     3181 cctttgccct caataatttc acaaaccgta gccctgaaac cgaatgtaag cttcattttg
     3241 ttcccttgaa agattgttac aaatatacaa aactttttat catcttaaaa tcgtatattt
     3301 gtaaatcata ccaaatagtc acctcgggat aatactattt taaaacagga aaacatggaa
     3361 aacaaattcc tctacagcgt tgcacgccat ttttacaaaa agcaggaaaa taggatcaat
     3421 aagctctgtt ttgtctttcc aaacagacgc tcgtcaatct ttttcaagaa gtatcttaca
     3481 caatgtatgg cgggtgaagc cctttttgct cccaagatga ttacaatcaa tgacttcttc
     3541 cttgagctct cgggagggca gttggttgac aagattgagg cgctttacat attgtatcag
     3601 cagtatgcta aggtattttc tgatgaagag ggtattgcaa aaagcacatt tgacgaattt
     3661 gtctattggg gggatgtctt tctgaatgat ttcgatgata tagacaaata tctggtcgat
     3721 gctgagaaac ttttcaccaa tatcggagat ctgcaaaagc ttacggcaga agagaacgat
     3781 tatatgagtg acgggcagaa gagagctata aaggagttct acacaagttg gaaccatagc
     3841 aaaaagaggg atatggtaag taaaaagaag gaggaggata atggagagaa agaagagagc
     3901 ggagatgatg ctgaaaatga ggagcagaaa gagaacggag agagggaaaa taatgagata
     3961 aacaaaggga atggagaaag ggataaaata gacatcaagg aggaattttc cacaatttgg
     4021 gagaacttgc acagacttta cacctcattc cgcgaagcgc tgaaggataa ggggctgatg
     4081 tatgagggaa tgatctatag agaggttgca gaagcgctga aagaagagat taaagagccg
     4141 caggaaaaag agagaaaaga agagatttta aaaaaatgtc gcaaatatga taaaatcatt
     4201 tttgtaggct taaacgccct gaatgagtgt gaaaagtctc ttttcaggca ccttaataag
     4261 cctgggagtg ggattgacgt agatttcatc tgggattatt atggcgatat cctcactgac
     4321 gaggccaata aatcttctca gtttatgcgc cgtggagata agggcaatgt cgtggaattt
     4381 ccacagaaag agtcgataga accctatgga acacctaaaa atcccaccca caatgctacc
     4441 tttggccccg gaaaaataga gatcataggc ataccctcag cagcaggtca gacgagatat
     4501 gtgacggaaa tactggaagg tattcactgt aataaagatg ggactcttct ccccaacctt
     4561 gacctcgaag agactgccat agttctaccg gatgaaagtc tgctccatcc aatgctcaat
     4621 tccattccga aatgcgttga taaggtcaat gtcactatgg gttatccatt gatcaactca
     4681 cacactgcca catttgtctc ccaattggaa gaactgcata aaaaaactcg tccaaataag
     4741 agtggtgggg tctcatttca ccataaaagc gtaatggcac ttctcagcca ccccttcctt
     4801 acatctttgc tccctgcgga ggggttaaag agcatcaaaa atgaaattat cggcagaaac
     4861 ctcgtatatg tcaatttaga gactcttgtg gaggaacaac ttgaaagaga cgataatcaa
     4921 gtcgatacaa acgagcaacc atttaagggc gataaacagc aaccctactt cggcaatcaa
     4981 ccgattgagg ccggtgagca acaactcagc aaaagtgagt tgtttaaggc aatattcagc
     5041 acgactcccg atcctgaaaa tgttgggaat tggcttgaaa agattctcca acttattcaa
     5101 gaaaacctgg atggaattga cagggaggct gcataccatt acctcagagt aatcaaacgc
     5161 atctccgacc ttaatctccc tttaacggac attaaaacct atttccgtct cttaaaacaa
     5221 atagtctcag tactatcagt cccatacaga ggcgagcctc ttcagggcat acagattatg
     5281 gggcctctgg agaccagagc tctcgacttc aaaaatgtga ttatactctc aaacaatgaa
     5341 ggaatcttcc cttcggcaaa tgtgagcaac tcctttatac catacaacct tcggcttggg
     5401 ttcggcctac caacttacga gtatcaggac tccatctcag cttaccactt ctatcgcagc
     5461 atctgcaggg cagaacacat ctggctcata tatgactccc gcacggaagg cactaaagtc
     5521 ggagaaccga gccgctacat ccatcagctc aaatacggct acaatatacc gcgggatgga
     5581 agaaggaggc tggatattaa gcagctggcc atctcctacg agatgaaagg caggaaggtt
     5641 aatagtctgg atgttgaaaa gagtcctgct ataatgaaga aactgcgtga aaaatatatt
     5701 atgaggcctg acggcgattt caacggcagt ttttccgcct ccactctcaa tgattatttc
     5761 aaatgccaaa gattgttcta cctcaaacac gtcgaaggaa tcggcgaaga ggatgaagtg
     5821 gtggaagagg tggacggagc acaattcggc acaatttttc actatgtgat ggagaaactc
     5881 tatttgccgg atgagaaatt ttgcggggtg aaatggaata aaagtcagat agaaggttta
     5941 aaaaaggaca aaacgaagat tgagaaactg atacttgaag gatttgccaa agcaagagaa
     6001 aaacggggat atagctatgc aaataacacc gtcagttatg atgatctctc cggacaggaa
     6061 aggatagtat tcgaattgat cgctgatttt gtgaaaaaaa ctctcgaaat tgatgaagag
     6121 tatgcccctt tcacattcaa ggatgctgaa atgaaaatca actatcaaat ggatattgac
     6181 aatatcggca ctgtgaaaat caaaggctcc atcgacaggg ttgatgaaaa gagtggtaca
     6241 atcaggataa tcgactacaa aacggggagt gtgagtggga aaatgcgcga tgaaaaggga
     6301 gccaaaccca atgatacgat acctaaactt ttcaaagaag aaaaaaatgg ctcttgcgtt
     6361 aaaaactcgg tttcattcca acttttactc tacaaaatgc tctatttgca ccagcctgat
     6421 ctaggaaatg gagataatat cctacctgct gtgattgcgc tacgacattt ccacgaaaat
     6481 aagggcgtac gctctcagga ggggcttaca aaggagatca tggatgaatt tgaggagaaa
     6541 gtgaaggaga aaattaagga gatattcaat gaagaggtac ctttcaaagc cataactttt
     6601 gatgacggag agtatggcgc ctgccgttac tgcaattacc aaaacatttg taaaatcttg
     6661 taaaacccac agacaatgtc cgatacaaaa gcaaaccctg agattatcat agcttctgca
     6721 ggcgcaggta aaacacataa actgactgag acctacctgg agatgcttga aaatagctat
     6781 gacgccggca agccacatcc ccatagaaac atccttgcag taacctttac caacaaggcc
     6841 actgatgaga tgaaagagcg catactggag aaactctttt ttaaagctgc aaatggggat
     6901 cataattcgg agaagctcct gaaggagatc ctgcacgact attccggctt taacgtaagt
     6961 acaatcgaca gatttttcca gttcgtaatg aagacctttg caagggagtt gggtgaatat
     7021 ggctcatacg atgtggaact tgactcagaa ggtgctacta tagaggcgat cgaccgtatg
     7081 atggactccc tcggggatga taaggataca accttgttga aatggcttgt agattactca
     7141 cgcagcatta ttgatgatgg ccgttcctgg gatgtaagaa aggaactcta taagttttcg
     7201 gatctgttct ataaagagga ttttcttatt aaaaaaagag gtgaagcggg atatctttat
     7261 aggctggatg atggttccgg aattgatcca aaaacggaaa tcaaaaggct cctgcaggaa
     7321 atcaatagaa ttacaacaaa gtttgaaagt gaagcaaagg tacttgctgg ggtgataatt
     7381 gcggagtatg atagagcgaa tgattccgtt atagaaatga aaagtaatcc cagaaaagga
     7441 ttcgtaaata tacaaaacgg caagcacacc aatagctccg atgaattata tattaaagta
     7501 tacgacaatt ctgaggagtg gtgtaaatca ctcaagacta aaaccggctt attaaaggtg
     7561 gatgattaca aaagcaatta tgcacccgac ctccaaacaa aagtcaacga attggaaaaa
     7621 cattatgacg ataattacat cacatacaac accgctgtaa ctattcgaaa aaacatctat
     7681 ctccttggac tcacaaccga cattttcaaa gaactggaca aatatctcaa ggaaaatcat
     7741 cttgtcctgc tcagcagcac agctgacatc ctaaacagaa taattgacag gagcgacact
     7801 cccttcatct atgaaagaat cgggaacagg atagaccata tattgctgga cgaatttcag
     7861 gatacatcaa gactacaatg gcaaaatttc gaacctctga tcgacaacaa catcgccaat
     7921 agcaatgaca accttatcgt aggtgatgtt aagcagagta tctacagatt cagaggttcc
     7981 gactcctctc ttctcaggga tctccctaaa aacaaaaatg ttacgcctga aattaaatat
     8041 gaaaactggc gtagcgccga tacaatagtc cgatttaata gcgatttctt tgaaactatc
     8101 acaaaaaatg atcctatcgt aaaagatatt tacacaggct gtagtcaggt aatgcccagc
     8161 aagtcaaaaa accaaggcaa cctcaaccgc tatgatgcag gagctgttaa agtcagagtt
     8221 tttgaagt
//
LOCUS       DAOSIE010000032         8159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_8202_length_8159_cov_3.289116, whole genome shotgun sequence.
ACCESSION   DAOSIE010000032 DAOSIE010000000
VERSION     DAOSIE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8202_length_8159_cov_3.289116"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLI69_01885"
     CDS             <1..441
                     /locus_tag="PLI69_01885"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HOG41275.1"
                     /translation="NIVTSLALDADVMEHHNRHLQAKYATIEAEEVRYETYKTEDAEY
                     LIVAFGCMSRISENVVELAREDGIKLGVLRPITLYPYPEKALDALVPQMKSIMSVELN
                     SGQMVEDVRRIAAGRVPVHFYGRQGGVIPSPEEIYEAFIKTIKK"
     gene            447..1208
                     /locus_tag="PLI69_01890"
     CDS             447..1208
                     /locus_tag="PLI69_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG41276.1"
                     /translation="MEINDIIKPKNKVYGKTPVLKDAVMHYCPGCSHGTVHKIIAEVI
                     EEMGIQDKTIGISPVGCSVFAYNYLDIDWQQAAHGRAPALATATKRLMPDKYVFTYQG
                     DGDLASIGTAEILHACNRGENIVVIFINNAIYGMTGGQMAPTTLIGQVTATTPYGRDV
                     KLNGYPLKITELLAQLDGTCYVTRQSVQTPGAVRKAKRAIRKAFENSMMDKGTSFIEV
                     VSTCNSGWKVSPVEANKWMEENMFPEYPLGDMKDR"
     gene            1246..1788
                     /locus_tag="PLI69_01895"
     CDS             1246..1788
                     /locus_tag="PLI69_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG41277.1"
                     /translation="MKEEIIIAGFGGQGVLSMGKILAYGGIMQDQEVTWMPSYGPEMR
                     GGTCNVSVILSDNKISSPILSRFDTAIILNQQSLDKFESRVKPGGVLIYDPNGITRHP
                     ERKDIKVCRIAAADEAAKLGNAKAYNMIVLGAYLKVKPLVTMDNVLEGLRKSLPERHH
                     GLIPMNEQAISIGMKAVEIL"
     gene            complement(2068..2370)
                     /locus_tag="PLI69_01900"
     CDS             complement(2068..2370)
                     /locus_tag="PLI69_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOG41278.1"
                     /translation="MEENKNTGISITPEVARGVYSNLVFINHSNSEFVLDFIQVMPGM
                     PQPNVNSRIILAPEHAKRLLKALEDNVGKYEMTFGEIVIREPQPANVPPMAFGGEA"
     gene            complement(2377..3915)
                     /gene="rny"
                     /locus_tag="PLI69_01905"
     CDS             complement(2377..3915)
                     /gene="rny"
                     /locus_tag="PLI69_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005848820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOG41279.1"
                     /translation="MFTTIIITAIVSAVVTIGAYLLVRDVILKKKKAQIIINAEKEGE
                     DIKKEKIFQAKEKFLQLKSEHETFINERNAQVQQYENKVKQKELVLNQQNSELGRKQK
                     EVDSIRENLKSQLDIVNRKSEEYDKYKNEVISKLEEVAEMTAAEAKNQLLESMKAEAK
                     TEAMSYISDVMDEARMTASKEAKRIVINTIQRTAPETAIENAVTVFNIESDEIKGRII
                     GREGRNIRALEAATGVEIVVDDTPEAILLSAFDPVRREVARLALHQLVTDGRIHPARI
                     EEVVAKVQKQLDDEIMETGKRTCIDLGVHGLHMELIRLIGRMKYRSSYGQNLLQHSRE
                     VANICSTMAAELGLNAKVAKRAGLLHDIGKVSDEDSELPHALLGMRIAERYKEKPEVC
                     NAIGSHHEEMEMKSLIAPLVMVGDAISGARPGARREVIESYIKRLKDMEGIALSHPGV
                     TKTYAIQAGRELRVIVGAEEVSDQQCDALSADIARKIQEEMVYPGQIKITVIRETRSV
                     AFAK"
     gene            complement(4177..4467)
                     /locus_tag="PLI69_01910"
     CDS             complement(4177..4467)
                     /locus_tag="PLI69_01910"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOG41280.1"
                     /translation="MKQRITIKIAGKEFELDVEAASEEKIRAAVKRINQRIFEKSNRF
                     PMVPREEILSVILLEEEVRLVEFETLRDKEKENLLQQLHTLDERLGEYLIGR"
     gene            complement(4473..4754)
                     /locus_tag="PLI69_01915"
     CDS             complement(4473..4754)
                     /locus_tag="PLI69_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41281.1"
                     /translation="MDGEVINSLKGKIGQLIARCEKLEKENVRFSEKISALEATNAKQ
                     IDIIKRLKEQTERQQLIEAFGNMPDRTEAKKRVSALIREIDRCINLLND"
     gene            complement(4803..6401)
                     /locus_tag="PLI69_01920"
     CDS             complement(4803..6401)
                     /locus_tag="PLI69_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOG41282.1"
                     /translation="MKRLLYFFPLLAVALLITSGCNRAPKDGIHSITVCTTTDLHGAY
                     FDSTYNEEARLSSLANVSAYVKQLRSKGIKLVLIDVGDNLQGDNAAYYCNYIDTVNVH
                     IFARAAEYIGYDAIVVGNHDIEAGHQVYDRFREGKIPYLAANAIIEGSDGEPYFQPYT
                     IVTRKGVRIAIIGMTNANIKSWLSEELWEGMDFLKISDIAQDWVDGVLEKESPHLVIL
                     ACHTGSGHGLPDIENEARFLASSVRGVDLVLAGHDHRAVSEIIDNPAGEVLLINAGDR
                     ATLLGEATFELEIKEGSVISKRVASSRLIPMADVEPDSRYSARFKKDFKAVYEFANRE
                     IGTISEEIYFADALVGPSSYIDLVHKVQLDASGADISITAPLSSSGMIPAGVIRYQDL
                     VLLYRYENSLFVVELTGAQIRDYLELSYYNWVNNIGPVYNFDSAEGIIYRVKKSAPRG
                     SMVEIISMSDGSPFDPSRSYKVAISSYRASGGGNLLRDGAGVSPDALSVVARYGDIRT
                     LIGEYISKVGTVVPETPTNWIFVK"
     gene            complement(6398..7591)
                     /locus_tag="PLI69_01925"
     CDS             complement(6398..7591)
                     /locus_tag="PLI69_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOG41283.1"
                     /translation="MSKKRFKIGLLPKVLIAIALGILFSLFLPEWAVRIFITINSIFS
                     NFLGLIIPLLIIGLIAPGIFELGKGAGKLLLITAAIAYFSTIFSGFFSYFTAKWSYPR
                     FLGDSLTSLGNIDMSGGLQPYFTIEMPPLMSVTTALVIAFVIGLGLTSVKGETMRNFM
                     LDFKEIIYAVIKGVIIPLLPLFIFGIFLQMGAEGKVSAIMGMFLKIIVIIFIMHVALL
                     VMQFVVAGAVARKNPFKALATMLPAYMTALGTQSSAATIPVTLAQTIKNGVREEVAGF
                     VIPLCATIHLAGSILKIVACAYAISLSMGMNVGLDLYAGFILMLGVLMVAAPGVPGGA
                     IMASIGLIASMLGFDPSMQGLMIALYIAMDSFGTAANVTGDGAIALVINTIRTKSSKE
                     SAVVQ"
     gene            7657..>8159
                     /locus_tag="PLI69_01930"
     CDS             7657..>8159
                     /locus_tag="PLI69_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG41284.1"
                     /translation="MGNTITFLGTGTSQGIPMIGCQCEVCSSSDVRDKRLRTSALVVY
                     EGVSILIDAGPDFRQQMLRENISHLDAIILTHQHKDHTGGLDDVRALNYLEKCATPIY
                     CEERVEESLRMEYSYVFARHKYPGIPEFNIKIIDSNPFTVTGGNGREVTITPIRVMHY
                     RLPILGFR"
BASE COUNT         2219 a         1900 c         1780 g         2260 t
ORIGIN      
        1 aacatagtaa cgtctcttgc ccttgatgca gatgtaatgg agcatcacaa caggcatctc
       61 caggccaaat atgccactat tgaggcagaa gaggtcaggt atgagaccta taagaccgag
      121 gatgcagagt atcttatcgt agcattcggc tgtatgtccc gtatatctga aaatgtagtg
      181 gaacttgccc gtgaggatgg cattaaactt ggcgtactgc gccccattac tctctatccc
      241 taccctgaaa aggcactcga tgcacttgta ccccagatga aatctataat gtccgtggag
      301 ctcaattccg gccaaatggt ggaagatgtg cgtaggatcg ctgccggccg cgtacccgta
      361 cacttctatg gccgccaggg aggtgttatt ccctctcctg aagagattta tgaagcattt
      421 atcaaaacaa tcaaaaaata gcgactatgg agatcaatga tataataaaa cctaaaaaca
      481 aggtttacgg taaaactccc gtacttaaag atgcagtaat gcactattgc ccgggctgct
      541 cgcacggcac ggtccacaag attattgccg aggttattga ggagatggga attcaggaca
      601 agaccatagg tatttctcct gtgggctgct ccgtattcgc ctacaactat cttgacatag
      661 actggcaaca agctgcacat ggacgtgctc cggcacttgc aactgccacc aaacgcctga
      721 tgcccgacaa atatgtattc acatatcagg gagacggcga cctggcttcg ataggtactg
      781 ccgagatttt gcatgcctgc aaccgtggag aaaatatagt agttatcttt atcaacaacg
      841 caatctacgg catgaccgga ggccaaatgg ctcctactac cctgatcggg caggtgacag
      901 ccacaacccc ttatggacgt gacgttaaac tcaacggcta ccctctaaaa atcaccgaac
      961 ttcttgcaca actcgatggc acttgctacg tgaccaggca atccgtacag actcccggtg
     1021 cggtacgcaa agccaagaga gctatacgca aagctttcga aaactccatg atggacaaag
     1081 gaacatcatt tatcgaggtg gtaagtacct gtaattccgg atggaaagtg tcgcccgtag
     1141 aggcaaataa atggatggag gagaatatgt tccccgagta tccccttggt gatatgaagg
     1201 atcgttaaga acctctagat tattgtgatt aataaaattg atgatatgaa agaagagatt
     1261 attatagcag gtttcggagg acagggagtc ctctctatgg gcaagatttt ggcttatggg
     1321 ggcattatgc aggatcagga agtcacttgg atgccgtcat acggtcctga gatgcgtggt
     1381 ggcacctgta atgtcagcgt aattctcagc gacaacaaga tcagctcgcc catactcagc
     1441 agatttgaca ctgcaatcat actcaatcag cagtcgctcg acaagtttga gagtagggta
     1501 aagccgggag gagtcctcat ttacgatcct aatggcatta cgcgccatcc cgaaaggaaa
     1561 gatataaaag tttgtcgcat agctgcggca gatgaggctg caaaacttgg caatgctaaa
     1621 gcctacaata tgattgttct tggtgcatac ctcaaagtga aacctctcgt gactatggat
     1681 aatgtactcg aaggactcag gaagtccctt ccggaacgcc atcacggtct gataccgatg
     1741 aatgagcagg caatcagcat cggaatgaag gcagttgaga ttttgtaaag gaatagatac
     1801 gaatagtaca aaaaatgggg gtcagcaata atgtgacccc catttttaat agttttaagt
     1861 aagtagtcgg tagttatgag taagtttttt agtagttagg tatgagttcg gaactagttt
     1921 ttggtttatg tagagagttc tgtgagactc caaatccttt gggattgtta taaaatttca
     1981 aaatcacaaa actttcttcc ttttccataa atcccaactc ctaaatccct tttactacac
     2041 actacacact acacactaca tactatttca cgcttctccg ccgaatgcca tcggaggcac
     2101 attggcaggc tgcggttcac gaataactat ctcaccaaaa gtcatttcgt atttgcctac
     2161 attatcctca agcgccttca aaagacgttt ggcatgttcc ggagccagga taatgcgact
     2221 attgacatta ggctgaggca ttcccggcat cacctgtata aagtcaagca caaactcaga
     2281 attggagtgg ttgataaata caaggttaga atagactccc cttgccactt cgggagtgat
     2341 actgattcct gtgtttttat tctcttccat gataatttat tttgcaaatg cgacactgcg
     2401 agtctcgcgg atgactgtaa tcttgatttg accggggtaa accatttcct cctgaatttt
     2461 gcgggctatg tcggcagaca gagcatcaca ctgctgatct gaaacctctt cagcccctac
     2521 tattacgcgc agttcccttc cggcctgtat ggcataagtc ttggtaacgc ccggatgtga
     2581 aagagcaatg ccttccatat cttttagacg tttgatataa gattcgataa cttcgcggcg
     2641 agcacccgga cgggcacccg aaatggcatc acccaccatt accagcggag ctatgagaga
     2701 tttcatctcc atctcttcat ggtgagagcc tatggcattg caaacttccg gcttctcctt
     2761 atatcgttcc gcaatacgca tacccagcaa agcatggggc agctccgaat cttcatcgga
     2821 aacctttcct atatcgtgca ggaggccggc acgtttggca acctttgcat tgagaccaag
     2881 ctcagcagcc attgtggaac aaatattggc cacttcccgg gagtgctgga gtaggttttg
     2941 tccataagag gaacggtact tcatacggcc tatcaagcgg atcaattcca tatggagtcc
     3001 gtggacaccc agatcgatac aggtgcgctt ccccgtctcc ataatctcgt catccaactg
     3061 tttctgtacc ttagccacaa cctcttctat gcgtgcaggg tggatgcgac catctgttac
     3121 caactggtga agtgcaagtc tggccacttc acgacggaca gggtcgaatg cagagagaag
     3181 aatggcttca ggtgtatcgt ccacaactat ttccacgccg gtggcagctt cgagtgcacg
     3241 gatattacgc ccttcacgac caatgatacg tcctttgatc tcatcacttt ctatattgaa
     3301 gacagtaacc gcattttcaa tggccgtctc aggtgcagta cgctggatgg tatttattac
     3361 tatgcgtttt gcctctttgc tggccgtcat acgcgcttcg tccatcacat cgctgatata
     3421 ggacatcgct tcggtcttgg cctcggcctt catactctcc aaaagttggt tttttgcctc
     3481 tgccgcagtc atttctgcaa cttcttcaag tttggaaatt acctcgtttt tatatttgtc
     3541 gtattcttcg ctcttgcggt ttacaatatc gagctgactt ttgagattct cgcgaatgct
     3601 gtccacctct ttctgtttac gacccagctc ggaattttgc tggtttaata ccagttcctt
     3661 ctgttttact ttattttcgt attgctgaac ctgagcattg cgttcattga tgaaggtctc
     3721 atgttcgctc ttgagttgga ggaatttttc cttagcctga aaaatcttct cttttttgat
     3781 gtcttccccc tctttttcag cgttgatgat gatttgtgct ttctttttct ttaatatgac
     3841 gtcccttacg agcagatagg ctcctatcgt aactacggca gaaactatcg ctgtaataat
     3901 aattgtggta aacatgattg taagtatata gtattaaata gtgcaaaaat tccctttttt
     3961 gaaaaaagcg aacaacagaa atacttacaa tttatagaaa agccgttagc ccggtctatc
     4021 agaaatctta taaaataaga tttgagctcc gatatttgtc tcaaacttcc tatgtcccat
     4081 aaatggaatc ccccttgcag ggtcacgaga ggcctgtttt taacaatcga gttggctcgt
     4141 gatgattttt agcgttgatt tcagcaaaga gcggaactaa cggccgatta aatactctcc
     4201 caatctttcg tccagggtat gcaattgttg caataaattc tctttctcct tatctctgag
     4261 tgtttcaaat tctaccaacc gaacctcttc ttccaggagt attacactca aaatctcctc
     4321 tctgggtacc ataggaaaac gatttgattt ttcaaaaatc ctttggttta tccttttgac
     4381 tgctgcccgt atcttctctt cactcgccgc ctcaacatcc aactcaaatt ccttccctgc
     4441 aatctttatg gtgatgcgtt gtttcatatt cttcagtcat taagcagatt tatgcatctg
     4501 tctatctcac gtataagggc cgaaaccctc ttctttgcct ccgtccggtc cggcatgttc
     4561 ccaaatgcct ctatcaattg ttgtctctct gtctgttcct ttaatcgttt tattatatca
     4621 atttgttttg cgttggttgc ttctagagca gagatctttt ctgaaaagcg tacgttctcc
     4681 ttctcgagct tttcgcatct tgcaatcaat tgcccgattt tgcctttcaa actattgatc
     4741 acttctccgt ccataataac gcaatgtaca aagataaata aaaatcccct atctgcaatg
     4801 atttatttta caaaaatcca gttggttgga gtttccggca ccacagtacc cactttagaa
     4861 atatactctc cgattagggt tctgatatcg ccatatctgg cgacaaccga cagagcatca
     4921 gggcttacac ctgctccatc ccttagcaga ttacctcctc cgctggctct gtatgaactt
     4981 attgccactt tgtagcttct tgagggatcg aatggtgatc cgtcactcat tgagataatt
     5041 tccaccatag agcccctcgg tgccgacttt ttcacacggt agatgatgcc ttcggcagaa
     5101 tcgaaattat aaacgggacc gatattattg acccagttgt aatatgagag ttccaggtaa
     5161 tccctgattt gagcacctgt gagttcaacg acaaacagtg aattttcata tctataaagt
     5221 aaaaccaggt cctgatatct gatgactccg gcaggaatca tgccgctgct cgaaagaggt
     5281 gcggtgatgg agatatcggc tccggatgca tccagctgca ctttgtgtac aagatctatg
     5341 tatgaggagg ggcccaccag tgcatcggca aaatagatct cttcagagat tgtaccgatt
     5401 tcgcgattag caaattcata gaccgccttg aaatcctttt taaaacgagc agaataacgg
     5461 gaatcaggct ctacatccgc catcgggatc aggcgtgaag aggcaactct ttttgaaatc
     5521 acactgccct ccttaatttc gagttcaaat gtggcctccc caagaagggt tgcacgatcg
     5581 cctgcattta tcaacaacac ctcaccggca gggttgtcga taatttccga aacggcacga
     5641 tggtcatggc cggcaagcac cagatccaca cccctgacac tggaagccag aaaacgcgcc
     5701 tcattttcga tatcgggaag accgtgtcca ctacccgtat ggcaggcaag gattacgaga
     5761 tgcggacttt ccttttcaag cacgccatca acccaatcct gtgcaatatc ggatatttta
     5821 aggaaatcca ttccctccca gagctcttcg gagagccaac ttttaatatt ggcattggtc
     5881 atacctatga tggctatcct gactcctttg cgagtaacaa tagtataggg ttggaaatag
     5941 ggttccccat cacttccctc aataattgcg ttggctgcaa gatagggtat tttgccctct
     6001 ctgaagcggt catagacctg atgtcccgcc tcaatatcgt gattgcccac tactatggca
     6061 tcgtagccta tgtattcggc agctcttgcg aaaatatgca cgtttaccgt atcaatatag
     6121 ttgcaataat aggctgcatt atctccctga agattgtctc ccacatctat cagtaccagc
     6181 ttgataccct ttgatctaag ttgtttgaca taggcagata cattggccaa cgaagatagt
     6241 cgcgcctctt cgttataggt agagtcaaaa taggcaccgt gcaggtctgt agtagtgcag
     6301 acggtaatgg aatgaatgcc atctttggga gcacggttac aacctgacgt tatcaggagt
     6361 gcaacggcca gtagaggaaa aaaatataat agacgcttca ttggacaact gctgattctt
     6421 ttgaagattt agttctgatg gtattgatga caagtgcgat ggcaccgtca cccgtgacat
     6481 tggcggcagt accgaagcta tccatagcta tgtagagggc gatcataaga ccctgcatgg
     6541 agggatcgaa gccgagcatc gaagcaatga gaccgataga ggccataatt gcacctcccg
     6601 gcacacccgg agcagctacc attagtactc ccagcattaa tatgaaacct gcatagaggt
     6661 caagtcccac attcattccc atactgagtg aaattgcgta agcgcaagct acaattttaa
     6721 gaatactgcc tgcaaggtgt atggtagcgc ataaggggat tacgaaacct gccacctctt
     6781 cacgcacccc gtttttgatg gtctgggcga gggttaccgg gatggttgca gcagaggatt
     6841 gagtgccgag ggcggtcata taggccggca gcatcgtagc aagtgctttg aaaggatttt
     6901 tgcgcgctac cgctccggct actacgaact gcatcacaag gagtgctaca tgcattatga
     6961 agataattac aatgattttg agaaacattc ccataattgc actcactttg ccttcggctc
     7021 ccatctggag aaatatacca aaaataaaga gtggcagaag tgggattatg acccctttga
     7081 ttactgcata tattatctcc ttgaaatcca acataaaatt gcgcatcgtc tcacctttca
     7141 cggaagttag gccgaggcct atgacaaaag ctatgaccag agccgtcgta acactcatca
     7201 gtgggggcat ttctatggta aaatagggct gtaaaccgcc ggacatatcg atattgccaa
     7261 gtgaggtaag cgaatccccc agaaaacggg gataactcca ctttgcggta aaataggaga
     7321 agaaacctga aaatatcgta gagaagtagg ctattgcggc agttataagc agcagtttcc
     7381 ccgcaccctt gcccaactca aaaatacccg gagctatcaa tcctataatg agcaatggta
     7441 tgatcagacc aagaaaattg ctgaagatgg aattgatggt tataaaaatc cttactgccc
     7501 attccggaag aaatagcgag aagagtatgc caagagcgat tgcaatcagc actttgggaa
     7561 gaagtccaat tttgaatctt tttttgctca tattcaaagg gtatattggt ctgtttgtta
     7621 cgcaaaaata atctttttta actatttttg taagttatgg gcaatacgat tacattttta
     7681 gggacaggca cgtcacaagg tatacctatg atagggtgcc aatgtgaggt ttgttcttca
     7741 tccgatgtaa gggataaaag actgaggact tcagcactgg tggtatatga aggagtttcg
     7801 attctcatag atgcaggacc tgacttcaga caacagatgc ttcgagaaaa tatttcccat
     7861 ctggatgcaa tcattcttac acaccagcac aaagatcaca cgggaggact tgacgatgtt
     7921 cgtgccctca actatttgga gaaatgtgct accccgattt attgcgaaga gagagtggaa
     7981 gagtctctcc gtatggagta ttcctatgta tttgcccggc ataagtaccc cggaatacct
     8041 gaatttaaca taaaaatcat agattcgaat ccattcacag ttacaggagg caacggtagg
     8101 gaggtgacaa tcactccgat aagggtgatg cactaccgtc ttcctatttt gggattccg
//
LOCUS       DAOSIE010000033         8157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_8206_length_8157_cov_3.848309, whole genome shotgun sequence.
ACCESSION   DAOSIE010000033 DAOSIE010000000
VERSION     DAOSIE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8206_length_8157_cov_3.848309"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /gene="mnmA"
                     /locus_tag="PLI69_01935"
     CDS             complement(<1..901)
                     /gene="mnmA"
                     /locus_tag="PLI69_01935"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOG41285.1"
                     /translation="MIPEKSHKIAVGLSGGVDSSVAALLLKRAGHDVFALFMQNWHDT
                     TGTLHGDCEWEEDRYVAEMVAKKINIPFHFVDLSEIYRERVVDYMFSEYEKGRTPNPD
                     VLCNREIKFDAFLKAALDLGADFVATGHYCRKDEFQDGHGNTIYRILSGKDSAKDQSY
                     FLCQLNQHQLSRALFPIGDLLKSEVRKLAREAQLPSAEKKDSQGICFVGKVDLPLFLQ
                     QKIQPKEGDVVEIFREHYESLNEPESLEELSRPHFYTPSQGKVIGVHRGAYFYTIGQR
                     KGLNIGGHKEPIFILSIDIDTNTI"
     gene            953..1052
                     /gene="ffs"
                     /locus_tag="PLI69_01940"
     ncRNA           953..1052
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLI69_01940"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1055..1531)
                     /locus_tag="PLI69_01945"
     CDS             complement(1055..1531)
                     /locus_tag="PLI69_01945"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOG41286.1"
                     /translation="MTERQLNIKYLEYSSAVKMSSEDRELLESAISAAEGAYAKYSSF
                     RVGAAVRMSNGVVVIGSNQENAAYPSGLCAERTALFYASARYPGESVEAIAVVALDDK
                     GVLTELTYPCGACRQVMAEYEMRAGREMRIIVGSAGVVQVFTGVRSLMPFIFDNLA"
     gene            1615..2553
                     /locus_tag="PLI69_01950"
     CDS             1615..2553
                     /locus_tag="PLI69_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOG41287.1"
                     /translation="MKRVICILVIALSQLLAGQSPRMSRSEYIARYSELAIKEMAISG
                     IPASITLAQACLESGNGNSYLAKEANNHFGIKCHGWKGETIKHSDDAPNECFRKYNHA
                     EESFRDHSDFLRYWDRYAFLFDLEPDDYKGWSYGLKKAGYATAPNYAELLISIIEEHQ
                     LYRYDTVSLIPPSPSEAEVSVRIHPTKGSPLYRVSLYREVYSRNKVAYIIATEYDTFK
                     SLAKEYDLFRFELYHFNDLDRKDKITPGMVIYLEKKRKESARHLDRHVVEEDETLWSI
                     SQRYGVRMKYLRKYNNLAPGTEPLPGSIINLRKRTL"
     gene            2550..3584
                     /gene="mltG"
                     /locus_tag="PLI69_01955"
     CDS             2550..3584
                     /gene="mltG"
                     /locus_tag="PLI69_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOG41288.1"
                     /translation="MKLYKKIILIVLISMAAAGALLVFGYYFIFHKNNIAEEGFIKVY
                     RNMDYGQLSRAIDTSGMLANKATFHFVAKAMGLEDSFKPGLYKFKKGMNNKAIVRSIA
                     HGWQTPTDVTISGYIRSKEKLAQILSSKLEADSALFMQVLCDTVLMHSFGFTEASYPG
                     MIIPNTYEMWWTVTPVEFLEKMKKEYDKFWNEERCAKADSIGLTKEQVSTLASIVIEE
                     TKYEPEMATIAGVYMNRLKKGMRLQADPTVLFAIGDRSIRRVLRTHLTVDSPYNTYKY
                     IGLPPGPITIPPISALDAVLNYEDHDYIYFCAKDTFDGQHSFAKDYKQHLRNAKAYQK
                     AFTARERSSF"
     gene            complement(3556..5136)
                     /locus_tag="PLI69_01960"
     CDS             complement(3556..5136)
                     /locus_tag="PLI69_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG41289.1"
                     /translation="MKRTLIIIFLLLPVIISAGHLSAQSNSARLKEVIEIISSRYIDT
                     IDIPNLVDNAIAKTVLELDPHSTFIPADQVEAANESLSSSFEGIGIEYLMMDDTLTVQ
                     SVITEGPSFSAGILPGDKFIRADGRTIAGASLPTTAIQKLLRGKKGTKVKIDLLRKGI
                     NDTLSYVITRDKIPVTSVDAAYMPEPGIVYVKLGRFAMESPLEVIKSIYDLSSEYPDG
                     VILDLRGNFGGYLHSAGLVANIFLSEGQLIFYTEGLNSPRIDFTARKQGLYPYGPLVV
                     LVDENSASSSEIVAGAIQDWDRGVVIGRRTFGKGLVQRTFTLSDGSQINLTIARYHTP
                     SGRVIQAPYEAGKREEYYRHQYTDRYASGEIFSRDSIRVNDSLEFTTLKLERKIYGGG
                     GIIPDLFVPRDTTGITPYYTSLVLRGIINDFVNRYVDAHRAAFDPALSFEKFYANYDL
                     EEELLAGLIEHARTLGLSPDYEQISSSLELIHTQLKALLARSLYGNTAYYRVINKEHD
                     LEFASAMEVIKKKTSPWR"
     gene            complement(5140..6075)
                     /locus_tag="PLI69_01965"
     CDS             complement(5140..6075)
                     /locus_tag="PLI69_01965"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOG41290.1"
                     /translation="MDFSLTTMGTASARPVANKYPSAHVVKIHGRLFLIDCGEGTQLQ
                     LSRYGFSHVKIDRIFISHLHGDHLFGIFGLLSTMALMGRTAPLYIYAPEGFEKVLVFF
                     MEHFGEGVIFDVIHVPLISAAPQLIFESRSVEVSAFGLNHRIDAFGFLFKEKEPARNI
                     HKWKIEVDNLTLAEIGTLKRGEDVVRSDGQLLKVEDYTYIPYKGRSMAYCSDTAPFPE
                     LASYIRGVDLLYHEATFAEDLHEMARWTFHSTGTQAASVAREAEVGKLVIGHFSSRYR
                     DLNVILKEAQAIFPETELAKEGMKFDIPLVRYSPE"
     gene            complement(6082..7980)
                     /gene="rpsA"
                     /locus_tag="PLI69_01970"
     CDS             complement(6082..7980)
                     /gene="rpsA"
                     /locus_tag="PLI69_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOG41291.1"
                     /translation="MNEELNNVAVEEQTPIETPELEETPIEAAEEAPKAAKKSNASVD
                     NSTFDWDAFESENVYDVDRADIEESYSQTLSRVNENEVVEGIVVAKDKREVVVNIGYK
                     SEGVINIAEFRYNPNLKVGDKVEVYVENAEDKKGQLILSHKRARSLRSWDRINEAYDK
                     EEVIEGFVKCRTKGGMIVDVFGIEAFLPGSQIDIKPIRDYDAYVNKTMTLKIVKINHE
                     FRNVVVSHKALVEAELEAQRAEMMKKLEKGNIVEGTVKNITSYGVFVDLGGIDGLIHI
                     TDLSWGRINHPEEVVELDQKINVFILDFDEQRKRIALGLKQLHPHPWDALDENLQVGD
                     KVVGRVVVIADYGAFVEIQPGVEGLIHVSEMSWSLHLRSAQDFLKVGEEVEACILTLD
                     REERKMSLGIKQLKPDPWATISEKYPINSKHTATVRNFTNFGIFVELEEGVDGLVHIS
                     DLSWTKKIKHPSEFTSIGDSLEVVVLEMDQDNRRLSLGHKQIEENPWDEYESLFTVGS
                     VHEGTVKAFVDRGATIILEHGIEGYASARNLVKEDGTTAKVDEVLQFKVVEFNKNSRK
                     IGLSHSKLFEEAKKEESREKRKESDSEATSTQKAVKKLKANLEKTTLGDISELAALKS
                     EMENRENE"
BASE COUNT         2168 a         1978 c         1775 g         2236 t
ORIGIN      
        1 atatcgtgtt ggtatctata tcaattgaga gtatgaatat gggctccttg tgaccgccta
       61 tattgagtcc tttacgctgg ccgattgtgt agaaatatgc cccccggtgg actccgatga
      121 cctttccctg cgaaggggta tagaaatgcg gacgggagag ctcttcaaga gactccggct
      181 cgtttagcga ttcataatgc tctcggaata tctccaccac gtcaccctct ttgggttgaa
      241 ttttttgctg aagaaatagc ggcaaatcta cctttccgac aaaacagata ccttgcgaat
      301 ctttcttctc agcactcggg agctgcgcct cacgcgccaa cttcctgact tcgcttttaa
      361 gcagatcccc tatcgggaaa agtgcccgac taagctgatg ctggtttaat tggcaaagaa
      421 agtaactctg atccttggca gagtctttgc cggataaaat gcggtatatg gtattgccgt
      481 ggccatcctg aaattcatct ttgcggcaat agtggccggt cgccacaaaa tctgccccga
      541 ggtcaagtgc cgcctttaga aaggcatcga atttaatctc acgattgcaa agtacgtcgg
      601 gattgggcgt acgtcccttt tcatattcac tgaacatata gtccacaaca cgttcacggt
      661 atatctcgct caaatcgaca aaatgaaagg gaatattgat ttttttggcc accatttccg
      721 ctacatagcg atcttcttcc cattcgcagt caccgtgaag agtcccggtc gtatcgtgcc
      781 aattttgcat gaatagggcg aaaacatcat ggccggcacg ctttaaaagc agcgccgcga
      841 cactggaatc cacgcctccg gaaaggccta ccgctatctt atgggatttt tccggtatca
      901 atgagagtca aaaaagattg ttaattcaaa aaaaatggct aattttgtag ttgggtaagt
      961 catatacgac cagctcccat tgaactcccc cagggtcgga aggcagcaag ggtaaatggt
     1021 tgtagcggtg cgatatatca agcttaccct ttttttatgc caaattatca aaaataaatg
     1081 gcattaaaga tctaactccg gtgaatacct gcaccacgcc tgcgctgccc acaattatcc
     1141 tcatttcgcg acctgcacgc atctcatact ccgccatcac ctgacgacag gcaccgcagg
     1201 gataagtcag ttcggtcaat acacccttat catccagagc cactacggca atagcctcaa
     1261 cactctctcc gggataacga gctgaagcat aaaatagagc cgtacgctca gcgcaaagcc
     1321 ccgaagggta tgcggcattt tcctgatttg aacctattac gactactccg ttgctcatcc
     1381 gcacagccgc tcccacccta aatgaggaat actttgcata ggctccctcc gctgcactga
     1441 tagcactttc caggagttct ctatcttcag aggacatctt tacagcggat gaatattcca
     1501 gatactttat attcaattgt cgttctgtca tacccgattt tctactttat tctgcgaatt
     1561 tatgacattt tttagttttg gccaaatgta ctacctttgt tgggttgaac cgagatgaaa
     1621 cgcgtaatat gcatattggt catcgctctt tcgcagctgc tcgcggggca atccccccga
     1681 atgagccgca gcgaatatat cgcacgttac agtgagcttg caattaaaga aatggccatt
     1741 tccggcattc cggccagcat cactctggcc caagcctgtt tggagtcggg caacggcaat
     1801 tcctatctgg caaaagaggc gaacaaccat tttggaatca agtgccacgg ctggaagggg
     1861 gagactatta aacatagtga cgacgcccca aacgagtgtt tcagaaaata caaccatgcc
     1921 gaggaatctt ttcgtgacca ctccgacttt ctaagatatt gggaccggta tgcatttcta
     1981 ttcgacctgg aacctgacga ttacaagggg tggagctacg gactgaaaaa ggcgggctat
     2041 gccactgcac ccaattatgc agaattattg atatccatca tagaagagca tcagctatac
     2101 cgatacgata ccgtatcgct aattcctccc tccccgagtg aagccgaagt ttcagtcaga
     2161 atacatccta caaaaggctc ccctctctat cgcgtctccc tttaccggga agtttacagt
     2221 cgcaataaag ttgcttacat catagctacg gagtatgaca cattcaaatc cctggcaaag
     2281 gaatatgacc tcttcagatt tgaactttat cattttaacg acctggacag aaaggataaa
     2341 atcactccgg gaatggtcat ttaccttgaa aagaaaagaa aggagagcgc aagacatctg
     2401 gatcgtcacg tagtagaaga ggatgaaaca ctttggagca tctctcaacg atacggagta
     2461 cgtatgaaat atctgcgaaa atacaataac cttgctcccg ggacagaacc tctccccggt
     2521 agtatcatca atttaagaaa aagaacccta tgaaacttta taagaaaata atcctgatcg
     2581 tattgatctc aatggctgct gccggagccc tcctggtttt tggttactac tttattttcc
     2641 ataaaaacaa tatcgctgaa gagggtttta taaaggttta tcgcaatatg gactacgggc
     2701 aattaagcag agccatcgat acatcgggaa tgctcgccaa caaagcaaca tttcattttg
     2761 ttgcgaaggc aatgggactt gaggactctt ttaagcccgg actatacaaa ttcaaaaagg
     2821 ggatgaacaa caaggctatc gtaaggagta tcgcacacgg ctggcagaca ccgactgatg
     2881 tgaccattag cgggtatatc cgttcgaaag agaaattggc tcagatactc tcctcaaaac
     2941 ttgaggcgga ttcggcgctc ttcatgcagg tattatgcga taccgtgctg atgcactcat
     3001 ttggcttcac ggaagcctct tatccgggaa tgatcattcc caatacttac gaaatgtggt
     3061 ggacagtgac accggtggaa tttctggaga agatgaaaaa ggagtatgac aaattctgga
     3121 acgaggaacg ttgcgcaaaa gccgattcca ttggtctgac aaaagaacag gttagcacac
     3181 tcgcctccat tgtcatagaa gagacaaaat acgaacctga gatggctacc attgccggag
     3241 tctacatgaa cagactaaaa aagggaatga ggctgcaagc cgaccctacg gtacttttcg
     3301 caataggcga ccgttccatc agaagagtcc tcagaaccca tctgaccgtg gactcaccct
     3361 acaataccta taaatatata ggacttcccc ccggccccat caccataccg cccattagtg
     3421 cactcgatgc cgtactcaac tatgaggatc acgattatat ctacttttgc gccaaagata
     3481 cctttgacgg ccaacattca tttgcaaagg attacaaaca gcatctgagg aatgccaaag
     3541 cataccaaaa ggcatttacc gccagggaga ggtcttcttt ttaataactt ccatcgccga
     3601 tgcaaactcc aggtcatgct ccttgttgat gacacgataa taagcagtat tgccataaag
     3661 agatctggca agcaacgcct tgagctgagt atggattaat tcgagcgatg aagaaatctg
     3721 ctcataatcg ggggagagtc ccaatgtacg ggcatgttct atcaatcctg ccagcaactc
     3781 ttcttccaag tcatagtttg cataaaactt ttcaaaggag agtgcaggat caaaagcggc
     3841 cctgtgggca tcaacatagc gattgacgaa atcattgata attcctctca acacaagaga
     3901 ggtgtagtaa ggagtaattc ccgtagtatc tctcgggaca aaaagatcgg ggattatgcc
     3961 gccccctcca tagattttcc tttccaattt tagagtggta aactcgagcg aatcattcac
     4021 tcttatgcta tccctgctga atatctctcc gctggcgtat cgatcggtat attggtggcg
     4081 gtagtattct tcgcgcttcc cggcttcata aggggcctga attactctac cggaaggagt
     4141 gtggtagcgg gcgattgtaa gattgatctg agagccatcg gaaagggtaa aagtacgctg
     4201 gacaagtcct tttccaaagg tcctgcgacc gattactact cctctatccc aatcttggat
     4261 tgctcctgca acaatttcgc tggaggaggc ggaattctca tctaccagca ccacaagtgg
     4321 gccgtacgga tagagtccct gcttcctggc agtaaagtcg attctggggc tgttgagtcc
     4381 ttccgtataa aatatcagct gcccctccga gagaaatatg tttgccacca atccggcaga
     4441 atggaggtag cctccgaagt ttcccctcag gtcgagaatt acgccgtcag gatattcgct
     4501 ggagagatca tatatggatt tgattacttc cagaggagac tccattgcaa aacgtcccaa
     4561 ctttacatag acaataccgg gttcgggcat ataggcggca tccacacttg tgacgggaat
     4621 tttgtctcgg gtaatcacat acgaaagtgt gtcattgatc ccttttcgaa gtaaatctat
     4681 ttttaccttc gtcccttttt tacctctgag gagcttttga atggcagttg tcggtagtga
     4741 ggctccggct atggtacgac cgtctgccct tataaactta tctccgggta agatgcctgc
     4801 tgaaaatgag gggccttcgg ttatcacgga ctgcaccgta agagtatcgt ccatcatcag
     4861 gtattctatg ccaattccct caaatgagct actgagtgat tcattggcgg cctccacctg
     4921 atctgccggt atgaaagtgg agtgaggatc gagttccagt actgtttttg cgatggcatt
     4981 gtccactaaa ttagggatat cgatagtatc aatgtatctg gagctgataa tctctattac
     5041 ctctttaagc ctggcactat tggactgagc tgagaggtgt cctgctgaga tgataaccgg
     5101 caggagtaaa aatattatga taagggttct tttcattctc tattcagggg aatatctgac
     5161 caatggtata tcaaatttca taccctcctt tgcaagttcc gtctcgggga aaatagcctg
     5221 tgcctctttg aggataacat tcagatctct gtatctggat gaaaaatgac ctatgaccaa
     5281 cttcccgact tccgcctctc gtgccacgga agcggcctga gttcctgtgg aatgaaatgt
     5341 ccaacgggcc atttcatgaa gatcctcagc aaacgtggcc tcgtgataga gcagatccac
     5401 gccacgtatg taagaagcca actccggaaa gggggcggtg tcactacagt atgccataga
     5461 tcgacccttg taagggatat aggtataatc ctcaactttc agtagttgcc catccgatct
     5521 tacaacatct tcacctcttt tcagagtccc tatctcagcc aatgtgaggt tgtcgacctc
     5581 tattttccat ttatgaatat ttcttgcagg ttctttttcc ttgaaaagaa atccgaatgc
     5641 atcaatcctg tgattcaggc cgaatgcact tacctcaacg gatctgctct cgaaaatgag
     5701 ttgaggtgca gcggaaatta gcggaacatg tattacatcg aatatgacac cctctccgaa
     5761 atgctccata aaaaagacaa gtaccttctc aaaaccttca ggtgcataaa tgtataaagg
     5821 tgcggttcta cccataagag ccatagtgga gagaagtccg aaaataccga acagatggtc
     5881 tccatggaga tgggagataa aaatcctgtc gattttcaca tgggagaaac cgtagcggga
     5941 gagctggagc tgtgtaccct caccgcaatc tatcaaaaac aaacgcccgt gtatttttac
     6001 tacatgggcg cttggatatt tattggcaac gggtcgggct gatgccgtcc ccattgtggt
     6061 tagagagaaa tccattcagg attattcgtt ctctctgttt tccatctcac tcttgagggc
     6121 agcaagctcc gaaatgtcac ccaaagtggt cttctcgaga tttgctttga gcttctttac
     6181 tgctttctga gttgaggtgg cctctgagtc gctctctttt cttttctcgc ggctctcttc
     6241 tttctttgcc tcttcaaata gtttggagtg tgacagaccg atcttcctgc tgttcttgtt
     6301 gaactccact actttgaact gaagaacttc atcaactttg gcggtagtac cgtcctcttt
     6361 tacgagattt ctggctgaag cataaccttc gattccatgt tcgagaatga tggtagcgcc
     6421 cctgtcaaca aatgccttga cagtaccctc atgaactgaa cctactgtaa agagtgactc
     6481 gtactcatcc cagggattct cctcgatctg cttgtggcca aggctgagac gacgattgtc
     6541 ctgatccatt tcgagaacca cgacctctaa ggaatcacca atggaagtga actctgaagg
     6601 gtgtttgatc tttttggtcc aggagagatc actaatgtga accagaccgt caacaccttc
     6661 ctcaagctct acaaatatac cgaagttggt aaaattgcga actgtagccg tatgtttgga
     6721 gttaatggga tatttctctg agatagtggc ccatggatcg ggtttaagct gcttgatacc
     6781 gagtgacatc ttgcgctctt cgcgatcaag tgtcaaaatg caagcctcta cctcttcgcc
     6841 aaccttcagg aagtcctgtg cgctgcggag gtggagtgac cacgacatct ccgatacgtg
     6901 aataagacct tctacaccgg gctgaatctc tacgaatgcg ccgtagtcgg caataaccac
     6961 cacgcgacct accactttgt cgcccacctg aagattctca tcaagagcat cccatggatg
     7021 tggatgaagt tgctttagac caagtgcaat cctctttctc tgctcatcga agtcgagaat
     7081 gaagacattg atcttctggt caagctccac tacctcttca ggatggttga ttctgcccca
     7141 tgaaaggtca gtgatatgga tgagaccgtc tatacctcca aggtcaacga atacgccgta
     7201 tgaagtgatg ttcttaaccg taccttcaac gatgttgccc ttttcgagtt tcttcatcat
     7261 ttctgccctc tgagcctcga gttctgcctc tacaagagcc ttatgcgata caacgacatt
     7321 acggaactca tgattgatct tgacgatctt cagggtcatg gttttgttga cgtatgcatc
     7381 gtaatccctg atgggcttga tgtcgatctg tgaaccgggg aggaaggcct ctataccgaa
     7441 tacgtccacg atcataccac ccttggtgcg acatttaacg aagccttcaa ttacttcttc
     7501 cttgtcgtat gcttcgttga tcctatccca tgaacggaga gagcgtgccc tcttgtggga
     7561 aaggatgagc tgtcccttct tgtcttccgc gttctccaca tatacctcaa ccttgtcgcc
     7621 tactttaagg ttgggattgt aacgaaactc ggcaatgttg attacacctt cgctcttgta
     7681 gccgatgttc acaacaactt ctcgtttgtc tttggcaacc actattcctt ccacaacttc
     7741 gttttcattt acgcgtgaaa gggtctggct gtaactttcc tctatgtctg ccctatcgac
     7801 atcatagaca ttttcacttt cgaatgcatc ccagtcaaat gtactgttgt caaccgatgc
     7861 attgcttttc tttgcggcct ttggagcctc ttctgcagcc tcaatgggag tttcttccag
     7921 ttccggtgtt tctatcggag tctgctcctc tactgcaaca ttgttcaatt cttcgttcat
     7981 tagttaataa ttagttttgt aacaagtagt tagactttat ttatagaacc ctttttccaa
     8041 gggcttgcaa aagtagtcac tatttcaatc aaatgcaaaa aatgtttgca tatttttatt
     8101 atttttgcct attacgaaaa ttcaaatccc ataaacacca tgaaccactt caaacat
//
LOCUS       DAOSIE010000034         8101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_8271_length_8101_cov_3.810216, whole genome shotgun sequence.
ACCESSION   DAOSIE010000034 DAOSIE010000000
VERSION     DAOSIE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8271_length_8101_cov_3.810216"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="PLI69_01975"
     CDS             complement(<1..151)
                     /locus_tag="PLI69_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HOG41292.1"
                     /translation="MKIAVAGTGYVGLSIATLLSQRHKVMALDIIPEKVEMINRRQSP
                     IQDEYI"
     gene            complement(173..1219)
                     /locus_tag="PLI69_01980"
     CDS             complement(173..1219)
                     /locus_tag="PLI69_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG41293.1"
                     /translation="MNILVTGAAGFIGANLIKEIFHRYDHPVVVGIDNINDYYDPHLK
                     EVRLEELSSYEEFIFIKGSIADKELIESVFKRYQPQIVVNMAAQAGVRYSITNPDAYI
                     ESNIIGFYNILEACRHSYENYEGGVRHLVFASSSSVYGSNKKVPYSTEDKADNPVSLY
                     GATKKSNELMAHAYSKLYDIPSTGLRFFTVYGPAGRPDMAYFDFTNKLVKGEKIQVFN
                     YGNCKRDFTYIDDIVEGVARVIQKAPERSVGEDGLPVPPYALYNIGNNAPENLNEFVS
                     ILQEELVRAGIVPADYDFDSHKELVPMQPGDVTVTFADTSALERDFCFKPSTPLRKGL
                     RAFVEWYKAYYFEK"
     gene            complement(1356..3539)
                     /locus_tag="PLI69_01985"
     CDS             complement(1356..3539)
                     /locus_tag="PLI69_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOG41294.1"
                     /translation="MNKMRKDYSNIRTMKQLELQIRQLEIEAIKGENKIREDINYAAY
                     FYSPGRLINSIGVYIKHFVMNFFRSMIIIAGLGVALTACSSREDQTDFTYSIDQFADI
                     EVLRYQVPNWDGLTLQQKEYVYHLSEAAKAGRDIIWVQNFKYNLPIRKVLEAIIEKCE
                     GKSGPEFDEFMIYAKRVFFSNGIHHHYALDKIIPGCSMEYFASLMDEAGYSDAKEELL
                     PVIFDPELYPQMVYQGTDKDLVAASAVNFYEGVTTAEVNAFYDAREVPGDPRPISYGL
                     NSRLVKVDGKIVEQVYKADGLYGEAIKVIIGHLEAAAEVAENDLQKRYIAELIEYYKT
                     GDLRMWDQYNVSWVSDVDSDVDFINGFVEDYSDPLGRKATYEGIVQYRDKEASKRTAL
                     ISENAQWFEDNSPIDPRFKKSEVKGVSAKVINVACIAGDNYPATAIGINLPNAAWIRK
                     EYGSKSVTIANITKAYDKATEQSPKSVLTEFAWDQDEIDFCKKYGSITGDLHTDLHEC
                     LGHGSGQLLPGVSPNALKEFSSTIEESRADLFALYYIADPKLVELGILDNPEAYKSEY
                     MNYIRNGSFTQFTRVELGKTNTQAHMQNRKLISDWAYENGLAENVIEKKVRDGKTYFV
                     INNFERLRELFGELLSEIQRIKSEGDYEAAKALVLKYAVNIDPDLHKEVRERYAQLEL
                     KPYRGFINPDIVPVEKDGKVVDYKIVYGDNFIEQMMEYGRKYATL"
     gene            complement(3532..3897)
                     /locus_tag="PLI69_01990"
     CDS             complement(3532..3897)
                     /locus_tag="PLI69_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41295.1"
                     /translation="MDPRTFLGPLEDIGNSAKDYADNRLDQLKLKTTKRLSASLAVLF
                     AWVIIVMVVLLAFSFLALALGTWLGEMLGSQVYGMLIISGIFLVIALVLFLLRKRIFV
                     NGLVRAFIGLFFEKDEKNE"
     gene            complement(3907..4197)
                     /locus_tag="PLI69_01995"
     CDS             complement(3907..4197)
                     /locus_tag="PLI69_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOG41296.1"
                     /translation="MKPSTIISFLTGAFVGVVAIAIYLSTDKGEETRVKIKNTLNDGM
                     DAAKRGCESAKRVVKDFAEDVEDKIHTESSDIRRRARRASDAAKAAVEESLR"
     gene            4272..5501
                     /locus_tag="PLI69_02000"
     CDS             4272..5501
                     /locus_tag="PLI69_02000"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOG41297.1"
                     /translation="MKKRGFIRILSLFALLVCISSCSLTSCKDNPEKEKVRSVLLYIS
                     ANNNLQSYAEECIRDLKLGFVPEYKDKSKILLVFYHIPGKAPTLSRYSCNKDGVVISE
                     TISIYEPEMNSSQITSFSRVLADVERSFPADYRGLILWSHATGWLPEGYFANPLDRIG
                     DKSYKPVEDPYRFLVKSSTDGTPQKSFGSDNNSEIEITDLAAALRHNYEFILFDCCLM
                     GGIEVAYEFKDKCEYMLFSPTEILADGFPYTKIFNPIFNVENRLEALKQVCSDYFAIY
                     DSKTGIYRSATVSMIKSSALDNLAGVCGSIYRNNQDSILTMDASQLQVYFRFDKHWYY
                     DFEDIVSRLATPTQYAEFKRALDQAIVYKAATDIFLDIDIERYSGLSTYLPRTQYKVL
                     NNFYKTLKWNIATNYVR"
     gene            5761..6339
                     /locus_tag="PLI69_02005"
     CDS             5761..6339
                     /locus_tag="PLI69_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOG41298.1"
                     /translation="MKQIELKGELRTTGRKADVKSIRRAGRVPCVLYGEGVENTSFSV
                     DAQDLKAITHTPNSYIINLNIDGKSQLAIFHAVQYHPVTDNTLHVDFLAVSADKPVTI
                     DVPVVITGSSEGVKMGGKLMVHSRKLRVSGLINELPDELKVDVTHLQLGKQIYAGNLS
                     FDKVQIVSPKNTLICAVRMTRSAIAAAAAKEE"
     gene            6355..6945
                     /gene="pth"
                     /locus_tag="PLI69_02010"
     CDS             6355..6945
                     /gene="pth"
                     /locus_tag="PLI69_02010"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOG41299.1"
                     /translation="MKYLVVGLGNIGAEYAGTRHNIGFMVLDAWAQASNTIFTVERYG
                     AVAEISFKGRKFILLKPSTYVNLSGKAVNYWLQKHKLTKENLIVVLDDLALPFGTLRL
                     RKKGSDGGHNGLKNIDYTLSSNDYARLRIGIGNGGFAEGGQVDFVLGEFILEEKRQLQ
                     TVLDTAINAIKCYGTEGIDQAMTLFNHPLSSTKKEE"
     gene            complement(6912..7307)
                     /locus_tag="PLI69_02015"
     CDS             complement(6912..7307)
                     /locus_tag="PLI69_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOG41300.1"
                     /translation="MDSTRIDKYLWAIRVFKTRADATEACRGGKVTVNGNEAKPSKEV
                     KTGDTISVRKGSVHYTYKVLSPLDKRQGAKEVEKYAQNLTPQSELDKLRAPTETFFIK
                     RDRGAGRPTKKERRQMDELYSSFFVDDNG"
     gene            complement(7312..>8101)
                     /locus_tag="PLI69_02020"
     CDS             complement(7312..>8101)
                     /locus_tag="PLI69_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOG41301.1"
                     /translation="IALERYDSYGKEEFWHILDADNDAVIYLGFKRKVTATEFYDRCK
                     DGTVIQLLNEIRPKKGDNFHIKPGTVHSASGGIRLVEIQQPSDITFRLYDWEREFDAL
                     TAREMHLEEAIDCIDYNASDPASLLIPTNPTRGPLLESDNFIISAVDLQGPRQIHTES
                     YGSFIIYVCTQGSASFQVMEGGRKASYDISKGGTILIPASLDDFSIVPLENKTLLLEV
                     WLRPAEMVDDYIDESVSDDCGDDDGSEFDEECGEDCHCGHNHKH"
BASE COUNT         2212 a         1880 c         1690 g         2319 t
ORIGIN      
        1 ctatgtactc atcctgaatc ggtgactgtc tccgattgat catttccacc ttttcaggaa
       61 ttatatccag agccatcact ttatgacgct gactcagaag tgttgctatt gatagtccga
      121 catatccggt accggctact gcaattttca tgtcttttat ctcttttatc gattattttt
      181 caaaataata tgccttatac cactctacaa atgcacgtaa accctttctt aggggtgttg
      241 agggcttgaa acaaaaatcc ctctccagcg cagaagtatc agcaaatgtc accgtcacat
      301 caccgggctg cataggcaca agttccttat gcgaatcaaa atcataatcg gcgggcacta
      361 tcccggccct taccaactcc tcctgaagaa ttgagacaaa ctcgtttaaa ttttcaggag
      421 cattattacc aatattgtaa agcgcataag gaggtacggg aagcccatct tcccctaccg
      481 acctctcagg agccttctgt ataactcggg ccacaccctc cactatgtca tctatataag
      541 taaaatcccg cttacagttg ccatagttga agacttgaat cttttcccct tttaccaatt
      601 tgttcgtgaa atcaaaatag gccatgtccg gacgaccggc tggaccataa acagtaaaga
      661 aacgcaatcc tgtggagggg atatcgtaaa gtttggaata ggcatgtgcc ataagttcat
      721 tgctcttttt tgtggcacca tagagcgata ccggattatc agccttatct tctgtcgaat
      781 aaggtacctt tttgttggat ccgtaaaccg aagaggaaga ggcaaacaca aggtggcgca
      841 cacctccttc gtaattttcg tatgagtgcc gacaagcctc aagtatatta taaaacccga
      901 taatattgga ctcgatatag gcatcaggat tggttataga gtagcgtaca ccggcctgtg
      961 ctgccatatt gactacgatc tgaggctggt atcgtttgaa aacgctttct atcagttctt
     1021 tgtccgcaat gctgccttta atgaatataa actcttcata cgaagacaac tcctccagcc
     1081 tgacctcctt gagatgggga tcatagtaat cgttgatatt gtcaatgccc accaccacag
     1141 gatgatcata tctgtggaaa atctctttta taagatttgc cccaataaat cccgctgctc
     1201 cggtcacaag tatattcatt ttcaaatctt gattcctgct tctttatctt acaaaattac
     1261 tcattttttt gacttgtagt catcaacata ataaaaaagc cccgatcagc ttttaccgtc
     1321 gggactttga gtaataagac atttctcaca atgagctaca aagtagcata tttgcgtcca
     1381 tattccatca tttgctctat gaaattatct ccatagacaa tcttgtagtc caccaccttc
     1441 ccatctttct ccacaggtac gatatcagga ttgatgaaac cacggtaggg cttaagctcg
     1501 agttgggcat atctttccct tacctcttta tggagatcag gatcaatatt aactgcatat
     1561 ttgagcacga gggccttggc tgcctcataa tcaccttccg atttaatcct ctggatttca
     1621 gagagaagct cgccaaataa ctctcttagg cgctcgaagt tgtttataac gaaatatgtc
     1681 ttaccgtcac gcaccttctt ttctatgaca ttttctgcca ggccgttttc gtatgcccag
     1741 tcagagatga gtttacggtt ctgcatatgg gcctgggtat tggtcttacc tagctctacc
     1801 cttgtaaact gtgtaaaact gccgttgcgt atatagttca tatattccga tttgtaggct
     1861 tcggggttgt cgagaatgcc gagttccacc aatttggggt ctgctatata atagagagca
     1921 aaaagatcgg cacgtgactc ctcaatagta gaactgaatt cttttagtgc attgggagat
     1981 acaccgggaa gcagctgacc tgaaccgtga ccgagacatt cgtgaagatc ggtatgtaga
     2041 tcacctgtta tagaaccata ttttttgcaa aaatcaattt catcctgatc ccaggcaaat
     2101 tcagtcagga cactcttagg actctgctca gttgctttat cataggcctt agtaatgttg
     2161 gcaatggtca ctgatttgga accatattcc ttacgaatcc aggcagcgtt gggtaagttg
     2221 atgccgatag ccgtggcagg atagttgtca ccggcaatac aggctacgtt aataactttt
     2281 gcggaaaccc cctttacctc agactttttg aaacggggat caattggtga gttgtcttca
     2341 aaccattgtg cattttcact gatgagtgca gtgcgttttg aagcctcttt gtcacgatat
     2401 tgaacaatgc cttcataggt tgctttacgg ccaagaggat cactgtaatc ctcgacaaaa
     2461 ccattgatga agtcaacatc tgaatccaca tccgataccc aggagacatt gtactgatcc
     2521 cacatacgga gatcgcctgt cttatagtac tcaataagtt ctgcaatata gcgtttctga
     2581 agatcgttct cagcgacctc tgcagcagcc tcaagatgtc ctataatgac ttttatggcc
     2641 tcgccataga ggccatcggc tttataaacc tgctctacta tctttccgtc cacttttaca
     2701 agacggctat tcagaccata agaaatgggg cgcggatcgc cgggtacttc gcgggcatcg
     2761 tagaatgcat tgacctctgc agtagtcaca ccttcataaa aattgacggc tgaggctgca
     2821 acaagatcct tatcagtccc ttggtacacc atctggggat aaagctcagg atcaaatatc
     2881 acagggagaa gctcttcttt ggcatcagag taccctgctt catccataag cgatgcaaaa
     2941 tattccattg aacatcccgg gatgatctta tccaaagcgt agtgatgatg tatgccgttg
     3001 ctgaagaata ctctcttggc ataaatcata aactcgtcaa actcagggcc tgattttcct
     3061 tcacatttct caattatggc ttccaacacc ttccggatcg gaagattgta tttaaaattt
     3121 tgaacccaaa tgatatcacg acctgctttg gctgcttctg agagatggta tacatactcc
     3181 ttctgctgga gggtaagacc atcccaattg ggaacctggt atctcaacac ctcgatgtct
     3241 gcaaactggt caattgaata ggtaaaatcc gtttgatcct ccctcgaaga acaggcggtt
     3301 agggctactc ctagccctgc gataataatc atacttctga aaaaattcat gacaaagtgt
     3361 ttaatatata ctccaatgga gtttatcaat cttccgggac tataaaaata ggcggcatag
     3421 ttgatatcct cgcgaatctt attttcgcct tttatcgctt cgatctctag ttgacgtatt
     3481 tgcaactcga gctgtttcat ggtcctgatg ttactgtaat ctttcctcat cttattcatt
     3541 tttttcatcc ttttcaaaaa ataagccgat gaaagccctt accagcccat ttacaaagat
     3601 gcgcttcctt aggagaaata gcacaagggc gatgacaagg aatatacctg aaattattag
     3661 catcccatat acctgggagc caagcatctc tccaagccag gtaccaagtg ccagagcaag
     3721 gaaagaaaaa gcgagcaata cgaccataac aattataacc caggcgaaca acaccgcaag
     3781 tgaggcggat agacgcttgg tagtcttaag tttgagctgg tcgagacggt tgtcggcata
     3841 atccttagcc gaattgccta tatcctcgag tggcccgaga aaagttcttg gatccatagc
     3901 aaaaaactat ctgagggact cttctacagc agccttggcg gcatcactgg cccttcttgc
     3961 cctacgtctg atatccgagg actcagtatg gattttatct tctacatcct cggcaaagtc
     4021 cttaactact ctctttgccg attcgcaacc cctctttgca gcatccatgc cgtcattgag
     4081 ggtgtttttg atcttgacgc gggtctcctc acccttgtcg gtcgacaggt atatcgcaat
     4141 tgcaactacg ccaacaaacg ctcctgtcaa aaatgagata atggttgacg gtttcataat
     4201 ctgtttgtat tatttgtgtt attgtttaga tacaaatata caaaatatat ttttttattt
     4261 acctttgcta tatgaaaaaa agaggtttta tccgcatttt atcgcttttt gcactattag
     4321 tgtgcatctc atcctgctcc ctcacttctt gcaaagataa tccggagaag gaaaaagtaa
     4381 gatcggtgct tctttatatc agtgccaaca acaatctcca aagctatgcg gaggagtgca
     4441 tacgagacct caagttaggc tttgttccgg aatacaaaga taagagcaag atattgctgg
     4501 tattctacca tattccgggt aaggctccca ccctctccag atacagttgt aataaagatg
     4561 gagttgtaat cagcgagacg ataagtatct atgaacccga gatgaactcc tcgcagataa
     4621 cttccttcag tcgtgtactg gccgatgtgg agcgtagttt ccctgctgac tatcgtggac
     4681 ttatcctttg gtctcacgcc accggatggc taccggaggg atatttcgca aatccattgg
     4741 accggatagg tgataaatct tacaaacctg tcgaagaccc ttaccgcttt ctggtaaaat
     4801 cctcaacaga tgggacgcca cagaaatcct tcggtagtga caacaatagt gaaattgaaa
     4861 tcaccgacct ggctgccgca ctccggcaca actacgagtt tatcctgttt gactgctgct
     4921 taatgggtgg tatcgaggta gcctacgaat ttaaggacaa gtgtgagtat atgctcttct
     4981 cacctacgga aatattggca gatggtttcc cgtacacaaa aattttcaat cctatattca
     5041 atgttgaaaa tcgcctggag gctctgaaac aggtatgctc cgactatttt gccatctacg
     5101 actccaagac aggtatctac cggtcggcta ccgtatcgat gattaagtcc agtgctcttg
     5161 ataatctggc gggagtatgt ggctccatct accgcaacaa tcaggattcc atcttgacca
     5221 tggatgcctc tcagctccag gtatattttc gcttcgataa gcattggtat tatgatttcg
     5281 aagacattgt ctcccgattg gcaactccca cacaatatgc agagtttaaa agagccctgg
     5341 accaggcaat agtctacaaa gcagcaaccg atattttcct cgacattgat attgagaggt
     5401 acagtggact gagtacctat ctgccacgta cacaatacaa ggtgctcaat aatttctaca
     5461 agacgctcaa atggaatatc gccaccaact atgttaggta gtatgtagta tgtagtatgt
     5521 agtatgtagt gagtagttgc ggggtgcggg gttaagttct gagtatggag tattgagtgt
     5581 gtagtaggta atttgcagtc tgaagtattt agttctgagt tttttgttaa aaaacaggca
     5641 aaatcggttt gatgttggga taatttgtta tctttgcgcc gctttaaaag gatctgttcc
     5701 ttttggtgca atggggtccg gaaagaccgg actgagtaac acattttaat tttatacaca
     5761 atgaaacaga ttgaactcaa aggtgagctc agaaccaccg gcagaaaagc agatgtgaaa
     5821 agcatccgca gggcaggtag agtaccatgc gtactttatg gagagggcgt agagaatact
     5881 tcattctcgg tagacgcaca agatttgaag gctattaccc acactcccaa ttcctacatc
     5941 attaacctta atatcgacgg taaaagtcag ctcgcgatat tccacgcagt acagtatcat
     6001 cccgtgactg acaatactct tcacgttgac ttcctggcag tttcagcaga caagcctgtg
     6061 actatagatg ttcccgttgt tataacgggc agttcggaag gtgtgaaaat gggtggtaag
     6121 cttatggtac actcacgcaa actacgcgtt tccggcttaa tcaacgaact tcccgacgaa
     6181 ctcaaagttg atgttacaca tctccagcta ggcaagcaga tttatgccgg aaacctctca
     6241 ttcgacaaag tacagatcgt ttcccctaag aatactctta tctgtgccgt aagaatgact
     6301 cgctcggcta ttgcggcagc agctgcaaaa gaagaatagt atatgatgta gtttatgaag
     6361 tatctagttg tcggactggg caatatcggt gcagaatacg ccggaacccg ccacaatata
     6421 ggttttatgg tattggatgc ctgggcccag gcatccaata ccatatttac ggtggagcgc
     6481 tatggcgcgg tcgccgaaat atcatttaaa ggaagaaaat tcatactgct taaaccctcc
     6541 acatacgtta atctaagtgg taaggctgta aattattggc ttcagaaaca taaactgacc
     6601 aaggaaaacc tcatagttgt attagacgac ctcgccctcc ctttcggtac tctacgtttg
     6661 aggaaaaagg gaagtgacgg tgggcacaac ggtctgaaaa acattgacta cacgctatca
     6721 tccaacgatt acgccagatt acgcataggt atcggtaacg gcggtttcgc cgaaggcggt
     6781 caggtagatt tcgttcttgg agaatttatc cttgaagaga aacgccaatt acaaaccgta
     6841 cttgatacgg caatcaacgc catcaaatgt tatggcacag aaggcatcga tcaggcgatg
     6901 accctcttca atcatccatt atcatccaca aagaaagagg aatagagttc gtccatctgg
     6961 cgacgttcct tcttcgtagg ccttcctgcc cccctgtctc gcttaatgaa aaatgtctcc
     7021 gtcggagctc taagtttgtc cagttcgctc tgaggagtga gattttgagc atatttttct
     7081 acctcttttg ctccctgtcg cttatccagt ggactaagta ctttataagt atagtggacg
     7141 gatcctttac gtacggagat agtatcacct gttttaacct ccttggaagg cttggcttca
     7201 ttaccattca ctgttacttt acctcctctg caagcctcag tggcatctgc acgagtctta
     7261 aagactctga tggcccagag atatttgtct atacgtgttg agtccatacc atcagtgttt
     7321 atggttatgc ccgcagtggc aatcctcccc gcactcctcg tcaaattcgg agccatcatc
     7381 atcaccgcaa tcgtcgctga cactttcgtc aatataatcg tctaccattt cagccggtct
     7441 aagccacacc tcgagcagta aggtcttatt ttcgagaggg actattgaaa aatcgtccag
     7501 agaagccgga ataagtattg tacctccctt tgatatatca tatgaggctt tccggcctcc
     7561 ttccattacc tgaaatgatg cggaaccctg cgtacaaaca tagattatga agctgccata
     7621 gctctcagta tggatctgtc ggggtccctg aagatcaact gcgctgatta taaagttgtc
     7681 actctcaagc aatggccctc gtgtaggatt agtaggtatc aggagagatg cggggtcact
     7741 ggcgttgtag tctatgcaat caatagcctc ctcaaggtgc atctctcttg cagtgagagc
     7801 gtcaaactcc cgttcccaat catacagacg gaaagtaatg tcggagggtt gttggatttc
     7861 caccaatctt ataccacctg atgcagaatg gacggtacca ggtttgatgt gaaaattatc
     7921 accttttttg ggacggatct cattgagcag ctggatcacg gttccatcct tacacctgtc
     7981 ataaaactca gttgcagtga cctttctctt gaaccccagg tatataaccg catcattatc
     8041 tgcatcgagg atgtgccaga actcctcttt accatagcta tcatagcgtt caagggcaat
     8101 t
//
LOCUS       DAOSIE010000035         8046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_8334_length_8046_cov_3.562633, whole genome shotgun sequence.
ACCESSION   DAOSIE010000035 DAOSIE010000000
VERSION     DAOSIE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8334_length_8046_cov_3.562633"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="PLI69_02025"
     CDS             <1..1052
                     /locus_tag="PLI69_02025"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOG41302.1"
                     /translation="ECDVVEEVLRIYGYNNIELPLNMKVAINRTPTPDPEALRSRISE
                     FLANNGFVETMNNSLTKSDYYSKLRTYPPERLVRLLNPLSHDLDSMRQTLILNALEVV
                     AYNLNRQITDLKIFEMGNVYSFVPKEGDDDPASNLKSYREEMRLSMVITGHGAKSWRN
                     ETGLGHYFYLKGYLELLLKRLGCNIYDLESDAAPSDIFSEGLEYSLQGKQIAVLGTIQ
                     PALARRYGIKQPVFVAEIMWDGILELLKRNKVRFKELPKYPEVRRDLALLLDESVSFA
                     ELRKCAFQTEKSLLRQVILFDVYRGDKIPSDKKQYALGFTLQHPDQTLTDADVEKAVD
                     KLLKAFQTKFGAILR"
     gene            1055..1930
                     /locus_tag="PLI69_02030"
     CDS             1055..1930
                     /locus_tag="PLI69_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HOG41303.1"
                     /translation="MYGFYKKVVNYLSFVKFSHTIFALPFALIGFVLAIREPEINFEW
                     LTLLKIVGCMFFARNSAMGFNRYADREIDARNPRTLSREIPSGKISPRQAIIFVIINV
                     ILFLLTALSINLLSFILAFPALAVLLGYSYMKRYTWCSHYVLGIALAIAPSAAFVAIT
                     GYFTPAILVLSLAVFLWSSHFDILYSLSDEETDRELGLHSVPQKFGRVKAMRISLAGH
                     LILFILIPLFGRLISGSWLFIIGACLFLALLVYQHAIISPTNLKRLNAAFFTSNGIAS
                     LLFMLFVVADLLFLQ"
     gene            complement(1935..3008)
                     /locus_tag="PLI69_02035"
     CDS             complement(1935..3008)
                     /locus_tag="PLI69_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG41304.1"
                     /translation="MKERVKVISAIQQVGIGVPDAQTAWKWYIEQFGIDIKVLAAEGV
                     AESMLPYTGGKPQPRYAILALNLRGGGGFEIWEPRGRELNWPASTPLLGDTGIFICKV
                     KCPDVQLAYNTYKSRGLDLLGAPCKSFAGLEHFYMKDPWGNIFEIEEDYFEFTKVKGS
                     ITGGSNGVVIGVTDMEKSIDFYGKLLGYDCKSADEKGVFDDLQGVPGSEQKLRRVMLE
                     RSQPLQGPFSDVLGTTHIELVQSLDRTPTKLYKDRFWGDPGFIHLCFDVRNMEAIRKD
                     SEEAGHPFVCDSGSAFGMGEANGHFTYIEDPDGTLIEFVETYKVPIHKKLGIYLNLAG
                     KDDRKPLPKIITKAFRFLRAKPE"
     gene            complement(3023..3832)
                     /locus_tag="PLI69_02040"
     CDS             complement(3023..3832)
                     /locus_tag="PLI69_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG41305.1"
                     /translation="MDKVRTKKFYTGKTALVTGAGSGIGEAISCNMAKNGANVIVTGL
                     GMEELERVCEICRGYGVKAWPFEFNLEKRETIDDLVSFMEKEELKPEVIVFNAGISQR
                     ALALDTDFSVDLKLMDINYLGSVYMTKKLAPMLKAAKCTHIGVNTSISGLFGFPLRSA
                     YSASKRALFGFFESLDLENDNIKVTFIIPGRVNTQISKSAILADGKPYAKMDPGQANG
                     MDVDRCGKIAVRAIAKGRHRKLIGGIELLMAYIYKYTPWLYWKLAKKVSAT"
     gene            3914..4840
                     /locus_tag="PLI69_02045"
     CDS             3914..4840
                     /locus_tag="PLI69_02045"
                     /inference="COORDINATES: protein motif:HMM:NF006263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOG41306.1"
                     /translation="MKIVFLDSATVGEDVSLRPISSLGELTTYSSTAPDEVVERIKGY
                     DVVITNKVWVGQKEIDSNPALKLICVAATGMNNVDLAYAASKQIPVRNVVNYSTESVA
                     QVTFMHILNLVGKAPYFDEAVKSGHYSVAGSFTDVTTPFYEIKGKRLGIIGLGNIGSR
                     VARIAEVFGMEISYYPTSGKAHCNVYPAKELKQLLAESDIITIHAPLNERTKGLITRK
                     ELEAMNPGGYIVNMGRGGIINEADLAEALDEGVIAGAAVDVFTTEPLPADHPYLTMKK
                     RDKLLLTPHIGWASKEARELLVAKIADNIKAL"
     gene            complement(4837..5622)
                     /gene="deoC"
                     /locus_tag="PLI69_02050"
     CDS             complement(4837..5622)
                     /gene="deoC"
                     /locus_tag="PLI69_02050"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOG41307.1"
                     /translation="MKEIYKNILGMIDLTSLNATDTPASVRAMVDKVNNFNDVFPDYP
                     KPASICVYPNFAGIIAATRRSRDIRITVVGGSFPASQSPLKVKLLECTEAVTDGADEV
                     DIVLALNSFLDNRPEEASREIYEINKAIKAINSDVHLKVILETGALKDAGLIEQASML
                     AMEAGADFIKTSTGKITPAATPEAALVMCQAIAKYYAKTGRKVGFKPAGGISTPEEAL
                     VYYNIVSDTLGTKWLTPALFRFGASRMANNILSALEEREIKYF"
     gene            complement(5630..6082)
                     /gene="dtd"
                     /locus_tag="PLI69_02055"
     CDS             complement(5630..6082)
                     /gene="dtd"
                     /locus_tag="PLI69_02055"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOG41308.1"
                     /translation="MRVVIQRVSSSSVTISGKVVAAIGSGMMVLVGFEEADTEEDIGW
                     MCRKIAALRIFDDENGVMNLSVTDTGGEVLVVSQFTLFASTKKGNRPSYIFAAKPETA
                     IPLYEKFKKVLSESIGKEVKCGEFGAEMEVALVNDGPVTIIIDSKNRY"
     gene            complement(6164..6940)
                     /locus_tag="PLI69_02060"
     CDS             complement(6164..6940)
                     /locus_tag="PLI69_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG41309.1"
                     /translation="MAIYFNTIVFGPIRSRRLGSSLGVNLLPLKGKLCSFDCVYCECG
                     WNADGRSDNKIPTVEQVREALKNKLEECHRDNIPVDSITFSGNGEPTIHPNFPEIIDY
                     TIALRNKFFPKAAISVLSNATMLGRPGIKEALRKVDNPILKLDGVDEAYVRLINRPQN
                     PDYSISEVISQLEWFEGNFILQTMFLRGVVDGVEVDSMRSDLLEGWQKMVLALRPREV
                     MLYTLDREPPLSTLKKVSVKEMEQAAAPLKEAGIKVRISG"
     gene            complement(6950..>8046)
                     /locus_tag="PLI69_02065"
     CDS             complement(6950..>8046)
                     /locus_tag="PLI69_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG41310.1"
                     /translation="SDSLDAQRKASAEYLEGILIPGFANAHCHVELSHMKGLFRQGTG
                     MDGFIQQINALRLTVNREERIAALTEQMEQMYEAGVSVMGDISNCDESFECKASSPIY
                     TRTYLEVFGTEPEDAPQILAGALQLQEKATRMGLDAAPTPHSPYTMSPLLNRLSAVEG
                     LKSGYLSYHNQESDQEEDMIRYGTGELAEDYKARGSSMPPVTGESALKYFLDNLQKEI
                     KAPIEGRLLLVHNTVTDQESIDEALKVLKSPYWAICPLSNIFIHRMLPPLDLLRENNL
                     TICVGTDSLSSNLQLSMVEEIRCIQEHYPHIPLEEILRWCCLNGACAVGAEERFGSFR
                     PGKKPGIVLIDNVDYKRMMLTKERKSRRIM"
BASE COUNT         1999 a         1922 c         1804 g         2321 t
ORIGIN      
        1 gagagtgtga tgtggtggaa gaggtgcttc gcatttacgg ttacaacaat atagagcttc
       61 ccctcaatat gaaggtggct ataaacagga ctcccactcc tgatccggaa gcgctccgtt
      121 cgcgcatatc ggaatttctt gccaacaatg gttttgtgga gacaatgaat aactccctta
      181 caaagagtga ctattattca aaactccgga cctatcctcc ggaaaggctt gtccggttgc
      241 tcaatccgtt gagccatgat cttgactcca tgcgccagac gcttatactt aatgcacttg
      301 aggttgtagc atataatctt aaccgccaga ttactgattt aaagattttc gagatgggaa
      361 atgtctattc ttttgttcca aaagagggtg atgatgaccc cgcttccaat ctaaagtcgt
      421 acagggagga gatgcgactc tccatggtaa ttacaggaca tggggccaaa tcgtggcgga
      481 atgagacggg acttgggcac tatttctatc tgaaaggtta tttggaactg cttctcaaga
      541 ggctgggatg taatatatat gatctcgaga gtgatgcagc tccatcggat attttcagtg
      601 aagggctgga atatagtctt caaggtaagc aaatagcagt tttaggtacg attcagcctg
      661 cattggcacg ccggtatggc atcaaacaac ctgttttcgt ggcagaaatt atgtgggatg
      721 gaattttaga attgctgaaa cgcaataaag tgaggtttaa ggaacttcca aaatatcctg
      781 aagtgcgtag agaccttgca ctgcttctgg atgagagtgt ctcttttgcc gaacttcgca
      841 aatgtgcttt ccagacggaa aagagtttgc ttcgtcaggt gatcctgttt gatgtctatc
      901 gcggagataa gatcccttca gacaaaaagc agtatgcatt gggtttcact cttcagcatc
      961 ccgaccagac tctcaccgat gctgatgtag aaaaggctgt tgacaagctc ttaaaagcct
     1021 tccagacaaa attcggtgct attttgagat agttatgtac ggtttttata agaaggttgt
     1081 caactattta tcctttgtga aattttcaca taccatcttc gcgctccctt ttgcgttgat
     1141 tgggtttgta cttgccattc gggaaccgga aataaatttc gagtggctaa cccttttaaa
     1201 gatagtgggt tgtatgtttt ttgcgcgcaa cagcgcaatg ggattcaacc gttatgccga
     1261 cagggagata gatgcccgta atccgagaac actctccagg gaaattcctt cagggaaaat
     1321 ctctccgcgg caagctatca tatttgtgat aatcaatgtc atactctttc ttttgactgc
     1381 tttaagcata aatctgctct cttttatatt ggcattcccg gcattggcgg tactgcttgg
     1441 ttatagttat atgaaacgct atacctggtg tagccattat gtcctcggaa tagcccttgc
     1501 cattgcccct tcggctgctt tcgtagccat tacaggctat ttcacaccgg ctattcttgt
     1561 gctttcgctt gctgtttttc tctggtcgtc acattttgat atcctctatt ctctgtcgga
     1621 tgaagagaca gatcgtgaat tggggctgca ctctgtgcct cagaagttcg ggcgtgtgaa
     1681 agcaatgcgg atttccctgg cgggacacct tattttattt atcttgatac cccttttcgg
     1741 ccggcttatt tcaggcagct ggctctttat aataggtgca tgcctatttc ttgcgttgct
     1801 ggtttatcag cacgcaatca tatctcccac aaatctgaaa agactcaatg ccgctttctt
     1861 tacatcaaac ggcattgcat cacttctctt tatgcttttt gtggtagctg accttctatt
     1921 tttgcaatag agtattactc cggctttgcc ctgagaaatc ggaatgcttt ggtaattatc
     1981 tttggtagcg gcttgcggtc atcttttccg gcaaggttga gatatatgcc gagtttcttg
     2041 tgaatcggca ccttataggt ctccacaaac tcaatcaacg taccgtcagg atcttcgatg
     2101 taggtaaaat ggccattagc ttcacccata ccgaaagccg atccgctatc acagacaaat
     2161 ggatgtcccg cctcttcgct atctttacgg atagcttcca tgttgcgcac gtcgaagcag
     2221 aggtggataa atcccggatc accccagaaa cgatctttat agagtttggt cggggtacgg
     2281 tcgagcgact gcaccaattc tatatgtgtg gtgccgagta catcactaaa aggaccttgt
     2341 aagggttggg aacgctccaa catcacgcgg cgcaacttct gctccgaacc cgggactccc
     2401 tgcagatcgt caaaaactcc tttttcgtca gcactcttac agtcatagcc gagtaatttt
     2461 ccataaaaat cgatggattt ctccatgtcg gtgactccaa ttacaactcc gtttgagcca
     2521 cctgtaatgg agcctttgac ctttgtaaac tcaaagtaat cctcctctat ttcgaaaatg
     2581 ttgccccacg ggtctttcat atagaagtgt tccagtccgg caaatgactt gcacggagca
     2641 cccagcaggt caagtcccct tgacttatag gtgttgtatg ccagttgaac gtcgggacat
     2701 tttactttgc agatgaaaat accggtatca cccagaagtg gggtagaggc gggccagtta
     2761 agctccctgc ctcgcggttc ccagatttca aatccgccgc ccccacgaag gttgagagcc
     2821 agaatggcat atcgtggttg aggtttacct cccgtgtagg gcaacatgct ctcggctact
     2881 ccttcggctg ccagtacctt tatatctatt ccgaattgtt caatatacca cttccatgcg
     2941 gtctgtgcgt caggcactcc aatgcccact tgctggattg cactaattac ttttactctt
     3001 tctttcatat ctttaaaggg tatcaggtgg cggaaacttt tttggccagt ttccagtaga
     3061 gccacggtgt atatttgtat atgtaggcca tcagcaattc gatgcctcca atgagtttcc
     3121 tgtgtcttcc ttttgcaata gcccttactg cgatcttacc gcagcggtcc acatccatac
     3181 catttgcctg tcccggatcc attttggcgt aaggcttgcc atcagccaaa atggcgcttt
     3241 tgctgatctg ggtgttgacg cgtccgggaa ttataaaggt aactttgata ttgtcgttct
     3301 ccaggtccag ggactcgaaa aagccgaata aagccctttt agaggcactg tatgccgatc
     3361 ttagcggaaa tccgaagagt ccggaaatgg aggtattgac accaatgtgc gtgcatttgg
     3421 cagcttttag catcggagca agttttttag tcatatagac tgaaccgagg tagtttatat
     3481 ccatcagttt gaggtctacc gaaaaatcgg tgtcgagggc gagtgctctc tgagagattc
     3541 ccgcgttgaa tactattact tccggtttta gctcttcctt ttccataaat gaaaccagat
     3601 catctatggt ttccctcttt tcaagattga attcgaaagg ccacgctttc acgccataac
     3661 cgcgacatat ttcacatacc ctctccagct cttccatacc caggcctgta acaatcacat
     3721 ttgcgccatt tttagccata ttacaggaaa tagcctctcc gataccggat ccggcacctg
     3781 taacaagagc tgttttgccg gtataaaatt tctttgttct cactttgtcc atagttttat
     3841 caaaatcaca caaagttagc aataataatt tgttattttt gtaaccgtat caaaacttga
     3901 ggaaatgaag aaaatgaaga tagtttttct agactcggct actgtaggtg aggatgtttc
     3961 cctccgtcca atctcatctc tcggagaatt gacgacctac tcttcaacag cccctgacga
     4021 ggtggttgaa cgtattaaag gatatgatgt tgtaattacc aataaagtct gggtgggaca
     4081 gaaggagata gattccaacc ctgccctgaa acttatctgt gtggctgcta cgggtatgaa
     4141 caatgtggat ctggcctacg ctgcttcaaa acaaattccg gttcgaaatg ttgtcaatta
     4201 ttccactgaa agtgtggcac aggtaacctt tatgcacatt ctcaacctgg tgggaaaggc
     4261 tccctacttt gacgaagcgg ttaaaagcgg ccactatagt gttgccgggt cttttaccga
     4321 tgtgacaact cccttttatg agatcaaagg caaacgtctg ggtatcatcg gacttggcaa
     4381 tatcggctcc agggttgccc ggatagccga agtcttcggt atggagatat cctattaccc
     4441 tacatcgggg aaagcacatt gcaacgtcta tcctgcaaaa gagctgaaac aattacttgc
     4501 tgagagcgat ataatcacga tacacgcacc tctaaacgaa cgcacaaaag ggttgataac
     4561 ccgcaaagag ctggaggcta tgaatccggg gggatatata gtcaatatgg ggcgcggcgg
     4621 catcatcaat gaggccgacc tggcggaagc tctggatgaa ggagtgatcg caggtgctgc
     4681 cgtggatgtc tttacaacag agcctctgcc ggccgatcat ccttatctta caatgaaaaa
     4741 gagggataaa ctgctgctta cccctcatat cggttgggcc agtaaagagg ccagagagtt
     4801 gcttgtggct aagattgccg acaatataaa ggctctctaa aaatatttta tctccctttc
     4861 ctccagtgcc gaaaggatat tgttcgccat cctgcttgct ccaaaacgga aaagcgcggg
     4921 agttagccat ttggtgccga gagtatcgct cacaatattg taatatacga gcgcctcttc
     4981 gggcgtggag ataccaccgg ctggcttaaa gccaactttt cttcctgtct tggcgtaata
     5041 ctttgcaatg gcctgacaca tcaccagggc tgcctccgga gtagccgccg gtgtgatttt
     5101 gccggtagac gtcttgatga agtcagctcc ggcctccatt gcgagcattg atgcctgctc
     5161 tatcagaccg gcatctttta gggcacccgt ctcgagaatt accttgagat ggacatcgga
     5221 gtttatcgcc tttatggcct tgtttatctc gtaaatctct ctcgaagcct cttccggacg
     5281 attgtcaaga aatgagttga gagcaaggac tatatccacc tcgtccgctc cgtcggtcac
     5341 cgcttcggta cactcaagca acttgacttt cagaggactc tgggatgccg gaaaagagcc
     5401 tccgactact gtgatacgaa tatccctgct tctacgtgtg gcagctatta ttccggcaaa
     5461 attgggataa acgcatatag aagcgggctt tggatagtcc gggaaaacat cgttgaaatt
     5521 gtttactttg tctaccatag ctcgtactga tgcgggagta tcggtagcat tgagagatgt
     5581 caggtcgatc atcccgagaa tatttttgta aatctctttc atatcacttt taatatcggt
     5641 ttttgctatc aataattatg gttacagggc catcattcac caaagcaacc tccatctctg
     5701 ctccgaattc gccacacttt acctctttac cgatgctttc ggagaggact ttcttaaact
     5761 tttcgtatag aggaatggca gtttcaggct tggctgcaaa aatataggag ggacggttgc
     5821 ctttctttgt agaggcgaaa agcgtaaact ggcttactac cagcacttca ccgccggtat
     5881 cggtcacgga gaggttcata actccatttt catcgtcaaa aattcgcaag gccgcaattt
     5941 ttctgcacat ccaacctata tcttcctctg tatccgcctc ttcaaagccc acaagtacca
     6001 tcatgccact acctatggca gctactactt taccgctgat agtgacactc gaagaagaga
     6061 ctctttgtat tactactctc atattgttta attttagttc ctttagttaa tattcaaata
     6121 acacggcttc gaaaaaataa caattttcta cgctccccac cccttatccg ctaatacgga
     6181 cttttattcc tgcctctttt agcggggcag ctgcttgctc catctccttt accgacacct
     6241 tcttcaatgt agaaagaggc ggctcacgat caagagtata taacataacc tcgcggggac
     6301 gcagtgccag gaccattttt tgccatcctt caagcaagtc gctcctcata gaatccactt
     6361 ctacaccatc caccactcca cgcaaaaaca tagtctgcaa tataaaattc ccctcaaacc
     6421 actccagctg cgaaattact tcgctaatgc tataatcagg attttgggga cggttgatta
     6481 gcctgacata agcctcatcc acgccgtcaa gtttgaggat ggggttatcc acttttcgta
     6541 gcgcctcctt tatcccaggt ctcccaagca ttgtcgcgtt tgaaagtact gagatagctg
     6601 ctttcgggaa aaacttattt ctaagggcta tagtgtaatc tatgatctca ggaaaattgg
     6661 gatgaatggt aggctctcca ttaccggaaa atgttatgga atccaccgga atattgtccc
     6721 ggtggcactc ttccaattta tttttaagtg cttcccgtac ctgctccacc gtaggaatct
     6781 tattatccga tcgtccatcc gcattccatc cgcactcgca gtagacacag tcaaaagagc
     6841 ataactttcc cttaagaggg agaaggttga ctccaagtga actgccgagt cgtctgctcc
     6901 tgatcggtcc gaaaactatt gtgttaaaat atatcgccat aattagtgat tacattattc
     6961 tccgggattt gcgctcttta gtgagcatca tccgcttata atctacattg tcaataagaa
     7021 ctatgccagg ctttttgccg ggtctgaatg atccgaagcg ctcttctgca cccacagcgc
     7081 aagcgccgtt gaggcagcac catctgagta tctcctcaag cgggatgtga gggtagtgct
     7141 cttgaatgca acggatttcc tccaccattg atagttgcag gttgctcgat aagctatctg
     7201 taccgacaca gatggtcagg ttgttttccc tcaaaaggtc aagcgggggc aacatcctgt
     7261 gtatgaatat attggaaaga ggacaaattg cccagtaggg gcttttcagt acctttagag
     7321 cttcatcgat gctctcctga tcggtaaccg tattatgaac cagcaacaaa cgtccctcta
     7381 taggtgcttt aatctctttc tgcagattgt caagaaaata ttttagtgcc gattcaccgg
     7441 ttaccggagg cattgagctg ccacgcgctt tataatcttc tgctagttct ccggtgccgt
     7501 atctgatcat atcctcctcc tggtcactct cctggttatg gtaggagaga tatcctgact
     7561 tgagtccctc cacagcagag aggcgattga gcaaagggct cattgtatag ggagagtggg
     7621 gggtaggggc agcgtccagt cccatccttg tagctttctc ttgtagttgg agtgctccgg
     7681 cgagtatctg cggagcatct tccggctccg taccgaacac ttccaaatag gtgcgggtgt
     7741 aaatgggtga ggaggcctta cattcgaagc tctcgtcgca gttgctgata tctcccatga
     7801 ccgaaacacc ggcttcatac atctgttcca tttgttccgt gagtgcggca atacgttctt
     7861 cccggtttac cgtcagacgc agtgcgttga tttgttggat aaatccatcc attcccgtac
     7921 cctgacggaa aagccccttc atgtgggaga gttcgacatg gcagtgggca ttggcaaatc
     7981 cggggatcag tataccctca agatattctg cagatgcttt tctttgtgcg tccagactat
     8041 ccgacg
//
LOCUS       DAOSIE010000036         7965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_8422_length_7965_cov_3.530215, whole genome shotgun sequence.
ACCESSION   DAOSIE010000036 DAOSIE010000000
VERSION     DAOSIE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8422_length_7965_cov_3.530215"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLI69_02070"
     CDS             <1..184
                     /locus_tag="PLI69_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOG41311.1"
                     /translation="IHTHFVCPVASKYGIDYLSKNLPHKKVTFWTAAIDEELTNKSYI
                     VPGLGDAGDLAYGDKI"
     gene            complement(185..1771)
                     /locus_tag="PLI69_02075"
     CDS             complement(185..1771)
                     /locus_tag="PLI69_02075"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG41312.1"
                     /translation="MKRIILPLFISLLLAGCGHKKQVDLIVINARIYTVDEDFSKAQA
                     FAVHNGKFVAVGSTRYIKADYKSDNVVNLRNSLATIYPGFNDCHAHLTSYGRGLLRVD
                     LRGATSYAEVVERLKQAQQKGGMTFVIGEGWDQNLWTDDNSMPTYHRLNEAFPDIPVI
                     IKRVDYHATVANKAAIDLAGMKDNGTGLFMEDAAAPLGNITPEYTPQELANIILAAQE
                     KCFAAGLTSISSVGESLKSLLVYDSLSNIGQLKLRIDAYLSASKANMEYFTSPYRNDR
                     LRIAGLKLFQDGALGSRGALLLKPYSDAPNIRGIHVTDDDTFRSYLKWADEHGFQVAT
                     HAIGDAANRYALDMYGEILKGENDRRWRIEHAQVVNPADMDKFGKFSIIPSVQPTHCT
                     SDMFWAEERLGDRIEHAYNFKQLMDQLGWIPTGTDFPVEDISPIKTFFAAVFRKNLDY
                     LPEEGFRMDEGLTREETLRSMTSWAAKVSFEEDVKGSIEPGKYADFIITDRDIMTVSE
                     REILSTKIIRTYVGGELVFK"
     gene            complement(1783..3159)
                     /gene="miaB"
                     /locus_tag="PLI69_02080"
     CDS             complement(1783..3159)
                     /gene="miaB"
                     /locus_tag="PLI69_02080"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOG41313.1"
                     /translation="MRDNKFHSVKPITDPTRPQLYIETYGCQMNVNDSEVVLSVMQDA
                     GYSLCEKMEDADVVLVNTCSIRENAEQRVFGRLDLFRLEKKRRPHIVVGVIGCMAERL
                     KEQLFECAVVDLVAGPDSYRDLPRLVEAAKAGAAQINVYLSHEETYADISPVRMDRNG
                     VSAFISIMRGCNNVCSYCIVPFVRGAERSRDPQSIVKEAENLIANGYKEINLLGQNVD
                     SYLWLDPDNPSRQVNFARLLEQIALIDRNVRVRFSTSHPKDMSNSVLYTMAMYDNICK
                     HIHLPVQSGSNAILEKMNRKYTRESYLERIDKIREILPKCAVTTDIIAGFCSETEQDH
                     LDTISLLEEVQFDGAFMFQYSERPGTRAARQFKDDVPPEVKTRRLNQIIALQNEISLR
                     NNQYLVGCEYEVLVEGTSKRSEEELMGRTSQNKTCVFPSAGHKDGDFVRVKVLSCTSA
                     TLICEAIS"
     gene            3208..3726
                     /locus_tag="PLI69_02085"
     CDS             3208..3726
                     /locus_tag="PLI69_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41314.1"
                     /translation="MRYKLLRNILLLLSIWLLIAGCQNYKKLQFKSYNIEKIDNLSFG
                     KGSVSATLTLRIGVANPTPSTFTAKELEATIYSGNGQPFAILTSGKSFVIPADSDDSI
                     PWEIETELLNPLSVLASGGFSLENLDVDNMTVDYSIVFYGGMRKKNTGKGVVLGDIIR
                     AFQATSPDTSPQ"
     gene            3735..4268
                     /locus_tag="PLI69_02090"
     CDS             3735..4268
                     /locus_tag="PLI69_02090"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG41315.1"
                     /translation="MKKAISTLIIVFCISLSTLLAQNQYIPQDTTINLSREVEVDTLL
                     AGRQIFSLLGESFPGGGTAIIRQSGTLRMALLNHISSNSGRKITGYRIRIYFDNSQNA
                     RVRSEAIANQFVREFPHIKAYLSHVNPYFKVTVGDFRTKYEAQQFASQISGRYPSVFI
                     VREQIGYPNVSTSDISL"
     gene            complement(4237..5196)
                     /locus_tag="PLI69_02095"
     CDS             complement(4237..5196)
                     /locus_tag="PLI69_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG41316.1"
                     /translation="MKPIIQPVDRKLIINELTPEKYIRDTNRGGNMLYEVSAADSPNI
                     MREIGRLREISFRSSGGGTGKDVDIDEYDTDPENPYKQLIVWDPDQMEVVGGYRYIDC
                     SGGLPPSKMATSELFNFSKHFVNDMLPYTIELGRSFVQPLYQRLNLKRKGIFALDNLW
                     DGLGALTIKYDTHRYFFGKVTMYSTYNVKARNILLYFLNHFFPDKDSLVTPIEPLDTD
                     ASNPYYASLFEGMEYKEAYKQLVARLREMGEMIPPLINSYMSLSPSMRVFGTSVNKEF
                     GGVEETGILMNIEDIYPEKIERHIASLKSLRDKIKEKYRWWRR"
     gene            complement(5165..6001)
                     /locus_tag="PLI69_02100"
     CDS             complement(5165..6001)
                     /locus_tag="PLI69_02100"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG41317.1"
                     /translation="MSQITPIKIDVRQVIAAKSPSLAKKLPRFVINRIKKLVHQDEIN
                     EILDLYADYEGVEFATRALSHLGIGYTSYGMEKIDPSGRYIFVSNHPLGGLDGSVLIA
                     LVGTMFPDIKFVVNDFLMHVKPFESLFIPVNKVGKMSREYAQQINDTYSSQSQILYFP
                     AGLCSRKIKGKITDTQWQTNFLKKAIEHKRDIVPIYFSGRNSNFFYTLAKIRKFIGIK
                     FNIEMMFLPRELFKQKNSSFTIWFGDPIPIESITPDKSLREWTDIIRTKAYEANNPTG
                     GS"
     gene            complement(6029..>7965)
                     /locus_tag="PLI69_02105"
     CDS             complement(6029..>7965)
                     /locus_tag="PLI69_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOG41318.1"
                     /translation="IKITALIAPGWHMYDMGPYENGPNATTFIFTLPKGVTKVGEISS
                     IEKPIKVNDPMFGMIIGYYEKSVTFVQKVKLSVPKATVEVLVEWMTCDDTSCTPPDDH
                     SMKVELLSSNASSLSQDAAAGGEESTLIQEDTEEATSELSSADTEVQISTPEKARRGG
                     SLWAMIIEAILWGFAALLTPCVFPMVPMTVSFFIKGSEDKVKGRFRAIMYGLFIVLLY
                     TVPIAGIIIVTRLVGGDAVTADIFNWLATHWLPNILFFLVFMVFAASFFGAFEIVMPS
                     KLVNKSDQQASMRQGLGGVFFLALTLVLVSFSCTGPIVGTVLIKSTAGEFWAPIITMM
                     AFSTAFALPFMIFALFPSLLGNIPKSGGWLNSVKVVLGFIEVALGFKFLSVADQVYHW
                     GLLDREIYLAIWIVCFTLLGFYLLGKLKFKHDSDLKHISVVRLVLAIASFSFVVYMIP
                     GMWGAPLKGLSGYLPPIGTQDFVIPTEGSSTPRSVEESTYTVKYGDFLKLPHGLNGFF
                     DLEEAQAHAKKVGKPVFVDFTGHGCVNCREMEARVWSDPRVLEILKRDYVICALYADD
                     KKQVPEENWVTNANGKVLKTLGKINSYFALTRYGVNAQPYYVLEDNDGRQLVEPRGYN
                     LDVESFILWLEEGVKAYKGI"
BASE COUNT         2135 a         1913 c         1775 g         2142 t
ORIGIN      
        1 gatacacacc catttcgtat gtcctgtcgc ttcaaagtat ggcatcgact acctctccaa
       61 aaatcttccc cacaaaaagg ttaccttttg gacggcagcc atagacgaag aacttaccaa
      121 caaatcttac atcgtcccgg gattgggaga tgcgggagac ctcgcttacg gcgacaaaat
      181 ctgattattt gaacaccaat tctcctccca cgtaggtacg gataattttt gtggagagga
      241 tttcgcgttc actgacggtc attatgtctc tgtcggtaat gatgaagtct gcatattttc
      301 cgggctcgat actccccttt acatcctctt caaaagagac ttttgctgcc caagaggtca
      361 ttgagcgcaa agtttcctct cttgtcaagc cttcatccat acggaatccc tcttcgggaa
      421 gataatcgag atttttgcga aataccgccg caaagaatgt cttgatgggg ctgatgtcct
      481 ccaccgggaa atcggtgcct gtgggaatcc atccgagctg atccatgagt tgtttgaagt
      541 tgtaagcgtg ttcaatccta tctcccaaac gttcttccgc ccaaaacatg tcggaagtgc
      601 agtgtgtcgg ctgtacggag ggtatgatag agaatttgcc aaatttatcc atatctgccg
      661 gatttaccac ctgagcatgt tcgatacgcc acctgcggtc attttctcct tttaggattt
      721 ctccgtacat atcgagtgcg tagcggttgg ctgcatcgcc gatagcatgg gtggccacct
      781 ggaatccatg ctcgtcagcc cactttagat atgaacggaa ggtatcgtca tcagtaacat
      841 gaatgccgcg tatattggga gcatcggaat agggtttgag caacaatgca cctctggagc
      901 cgagagctcc gtcctggaag agcttaaggc ctgcaatgcg caagcggtca ttgcgatagg
      961 gagaggtgaa gtactccata ttggcttttg aggccgagag atatgcatct atacgcaatt
     1021 tgagttggcc aatattggag agcgaatcgt atacgagaag actttttaga gactccccca
     1081 cggaagagat agaggtcaga cctgcggcaa agcacttttc ctgagcggca agtatgatat
     1141 tagccaattc ctgaggagta tattcgggtg taatattccc cagcggagct gcggcatcct
     1201 ccataaatag gcctgtaccg ttgtctttca tccctgcgag gtcaatggca gccttgttgg
     1261 caacagttgc atgatagtcc actctcttga tgattacagg gatgtcggga aaagcctcat
     1321 tgagacgatg gtaagtaggc atcgaattgt catccgtcca aagattctga tcccatcctt
     1381 ctccaatcac aaaggtcatc ccacccttct gctgtgcttg cttcaagcgc tccaccactt
     1441 ctgcatagga tgtagcccct ctcaggtcca ctctgagcag tcccctaccg tacgaggtca
     1501 gatgggcgtg acaatcatta aatccgggat atattgtggc caggctattc ctgaggttga
     1561 ctacattgtc cgatttataa tcagctttga tatatcttgt ggagcctacg gcaacaaatt
     1621 tgccgttatg gacggcaaat gcctgcgctt tggagaaatc ttcatctaca gtatagattc
     1681 tggcatttat gacaatcaga tctacctgct tcttgtgacc gcaacccgcg agcaaaagag
     1741 aaatgaaaag aggaagaatg atgcgtttca tatagttgat ttttaagaaa ttgcttcaca
     1801 aatcagagtc gcagaggtac aggagagtac ttttactctt acaaagtccc cgtctttatg
     1861 gccggctgac ggaaagacac aggtcttgtt ctgagaggta cgacccatca attcctcttc
     1921 agagcgtttg gaagtgccct ctaccaacac ctcgtattca catcctacaa gatattggtt
     1981 attacgtaga gaaatttcat tctgcagggc aataatctga tttaaacgcc gggttttgac
     2041 ctccggtgga acatcgtcct tgaattgtct cgctgccctt gtgccgggtc tttcggagta
     2101 ttgaaacata aatgcaccgt caaactgaac ctcttccaat agagagatgg tgtccagatg
     2161 gtcctgctct gtctcggaac aaaatcctgc gatgatatcg gtagtgacgg cacacttcgg
     2221 aaggatttcg cgaatcttat ctatacgctc cagatagctc tcacgggtat attttcggtt
     2281 catcttctcc aaaatagcgt tgctgcccga ctgcacgggg agatgaatat gtttacagat
     2341 attgtcatac atcgccatgg tgtataacac gctgttgctc atatccttgg ggtgcgaagt
     2401 agagaagcgt accctgacat tgcgatctat aagggctatc tgttccagca agcgtgcaaa
     2461 atttacttga cgagagggat tgtcagggtc taaccaaagg taagaatcca cattttgtcc
     2521 gaggaggttt atctccttgt atccgttggc tatgagattt tccgcctctt tgacgatgct
     2581 ttgcgggtcg cgactccgtt ctgcgccacg cacaaaggga actatgcagt aggagcaaac
     2641 attgttgcaa cctctcataa ttgagataaa tgcacttaca ccgttgcggt ccatccgcac
     2701 cggtgaaata tccgcatagg tctcctcatg agatagatat acattgattt gagctgcacc
     2761 agcttttgct gcctccacga gacggggaag atcacgatat gaatcagggc ctgcaaccaa
     2821 atccactact gcacattcga acagttgctc cttgagacgt tccgccatac agccgataac
     2881 tcctaccaca atatgaggac ggcgtttctt ttccagacga aacaggtcaa ggcgtccgaa
     2941 aacccgctgc tcggcatttt ccctgatgga acaggtattt acaagtacca catcggcatc
     3001 ctccatcttt tcacatagtg aataaccggc atcctgcatt actgagagta ccacctcgct
     3061 atcgttgaca ttcatctggc aaccataggt ctcgatgtac aactgtggcc tggtggggtc
     3121 tgttatcggt ttaacggaat gaaatttgtt gtctctcata tcgcaaagat aagtttttta
     3181 tatctttgcg gaaactaaag agcttatatg agatacaaat tattgcgaaa catcctgctg
     3241 ttgctctcaa tctggctgct gatcgccggc tgccagaact ataaaaagct ccaattcaaa
     3301 tcctacaata tagagaaaat agataacctg tcatttggaa aaggtagtgt cagtgctact
     3361 cttacactta gaataggcgt ggccaatccc actccttcca cctttactgc caaggagctt
     3421 gaagccacaa tttatagtgg caatggccaa ccttttgcaa tccttacttc cgggaagagt
     3481 tttgtcatac cggccgatag tgatgattcc attccatggg agatagagac ggagttgctc
     3541 aatcccctat cggttcttgc atccggtggt ttttctctcg aaaatctgga tgtggataat
     3601 atgacggtgg actatagtat agttttttac ggcggtatga gaaaaaaaaa taccggtaaa
     3661 ggggtcgtac tcggcgatat catccgagcc ttccaagcta cctcacccga tacatcaccc
     3721 caatgaaaat ccttatgaaa aaagcaattt ccacactaat aatagttttc tgcatctcat
     3781 tgagcactct cttggcgcag aatcaatata tccctcaaga taccaccatc aatctgagtc
     3841 gtgaagttga ggttgacact cttcttgcag gccggcagat attttcgctc ctcggcgaat
     3901 cctttcccgg aggcggtact gctataatca gacaatcagg cactctgcgc atggcacttc
     3961 taaaccatat aagctccaac tccgggcgca aaattaccgg ctatcgcatc aggatctatt
     4021 ttgacaacag tcagaatgcg agagtacgat ctgaggcaat agcaaatcag tttgtgaggg
     4081 aatttcccca tataaaggca tatctcagcc atgtcaatcc ctacttcaag gtgactgtgg
     4141 gtgatttccg caccaaatat gaggcgcagc aatttgcaag tcagataagt ggcagatacc
     4201 cctccgtatt tatagtacga gagcagattg gttatcctaa cgtctccacc agcgatattt
     4261 ctctttgatt ttatctctaa gagacttgag tgaggcaatg tgtcgctcaa tcttctcagg
     4321 atagatatct tcgatattca tcaaaatacc agtttcctca actcctccga actccttgtt
     4381 gaccgaggtt ccaaatactc gcatactggg tgaaagactc atgtaggagt tgatgagcgg
     4441 agggatcatc tcacccatct ccctgagacg ggctacgagc tgtttgtatg cctccttata
     4501 ctccatcccc tcaaaaaggg atgcatagta agggttggat gcatcggtat ccagcggttc
     4561 tatgggagtc acaagcgaat ctttgtcggg gaaaaaatga ttgaggaaat agagcaaaat
     4621 gttacgtgcc ttgacgttgt aagtagagta catcgtcact ttgccgaaga aataacggtg
     4681 cgtatcatac tttatcgtga gggccccaag accatcccaa agattatcta gggcaaagat
     4741 acctttgcgc ttgagattca gacgttggta gagcggctgt acgaatgagc gtccaagctc
     4801 aattgtatag ggcagcatat cattgacaaa gtgtttggaa aaattaaaaa gctcacttgt
     4861 ggccatcttt gacggaggca ggccaccgga acaatctata tagcggtatc cgccaacaac
     4921 ctccatctgg tcgggatccc aaactatcag ctgcttatag ggattttccg ggtcggtatc
     4981 gtactcatca atatccacat ccttgccggt gccgccgcct gagctacgga aggaaatttc
     5041 tcgcaagcga ccaatttcgc gcattatatt tggagaatcg gctgctgata cctcatagag
     5101 catattaccg cctctattag tatccctgat gtatttttcc ggagtgagtt cgttgataat
     5161 cagtttacga tccaccggtt ggattattgg cttcataggc ttttgttctt atgatgtcag
     5221 tccattctcg cagcgattta tccggagtga tgctctcaat ggggatcgga tcaccgaacc
     5281 agatcgtaaa gctgctgttt ttctgtttga atagttcgcg gggaaggaac atcatctcga
     5341 tattaaattt tataccgata aatttcctga tcttcgccag agtgtaaaag aaattggaat
     5401 tgcgtccgga aaaatagata ggcacaatat ccctcttgtg ttcaatggcc tttttaagaa
     5461 aattggtttg ccactgggta tccgtgattt tgccctttat cttcctggag cagagtcctg
     5521 ccgggaaata gagtatctga gactgtgatg agtaggtatc gttgatttgc tgcgcatatt
     5581 ccctactcat ctttcctact ttgttcaccg gaataaaaag cgactcaaag ggctttacat
     5641 gcattaagaa atcattcaca acaaatttta tatcaggaaa catagtgcct acgagggcta
     5701 tcaacactga accgtccagt cctcccagag ggtgatttga gacaaatata tatctgcctg
     5761 aaggatcaat cttttccatc ccgtaagaag tgtagccgat accgagatgt gagagtgcac
     5821 gcgtagcaaa ctcaacccct tcataatctg catataggtc aagaatctcg ttgatctcat
     5881 cctgatgaac aagtttctta atccggttga ttacgaacct gggaagtttt ttcgccagtg
     5941 aggggctttt cgccgcaatc acttgacgca catcaatctt tatgggtgta atctgactca
     6001 ttgtctcatt taattgcgac tacatccgtt atataccttt gtacgctttg actccctctt
     6061 caagccagag aatgaaggac tctacatcaa gattataacc gcgaggctcc accaactgtc
     6121 tcccatcatt gtcctccagg acataatagg gctgagcatt tactccatat cttgtcagcg
     6181 caaagtaaga gttgatctta ccgagggttt taagcacttt accattggca tttgtaaccc
     6241 aattctcctc cggaacctgt tttttgtcat cggcatatag ggcacaaatc acataatccc
     6301 ttttaagaat ctcaagtacc ctggggtcac tccataccct tgcctccatt tcgcggcagt
     6361 tgacacatcc gtgaccggta aaatctacga agaccggttt gcctaccttt ttggcgtgag
     6421 cctgagcctc ctcgagatca aaaaatccgt tcaatccgtg agggagctta aggaaatctc
     6481 catacttcac ggtataggta ctctcctcaa cactccgggg agtgctactt ccctccgtag
     6541 gtatcacaaa atcctgtgtg ccgataggcg gcagataacc cgataagccc ttgagaggcg
     6601 caccccacat accgggaatc atatagacta caaaactgaa cgatgctatg gcgagcacca
     6661 ggcgaacaac gctgatatgc ttcaagtcgc tatcgtgttt gaacttgagc ttgccgagca
     6721 ggtaaaatcc caacaatgta aagcagacaa tccagattgc aagataaatc tccctatcca
     6781 ggagtcccca gtggtagacc tgatcggcaa cgctgagaaa tttaaatcca agcgccacct
     6841 caataaatcc gagtaccact ttaactgagt tgagccaacc accactcttg gggatgttac
     6901 ccaacaggga ggggaagagg gcaaaaatca tgaatggaag cgcaaaggct gtagaaaagg
     6961 ccatcatagt gatgatcggg gcccaaaact caccggcggt agactttata agtactgtgc
     7021 ctacaattgg gccggtacag gagaaagaga ccaacacaag agtcagggcg agaaagaata
     7081 cccctcccaa accttggcgc atactagcct gctgatcgct cttattgacc aacttgctag
     7141 gcattacgat ctcgaaagca ccgaaaaatg aagccgcaaa taccataaat accaggaaaa
     7201 agaggatatt gggcagccag tgggttgcca gccaattgaa tatatcggca gttactgcat
     7261 ctcctcccac caacctcgtt acgatgataa tacccgctat ggggactgta tagagcaaca
     7321 caatgaaaag gccatacata atagcacgga accttccttt taccttatcc tcagaacctt
     7381 taataaaaaa agagacggtc atgggcacca tagggaaaac acaaggagtc aatagtgcgg
     7441 caaaacccca tagtattgcc tctattatca tagcccaaag agaaccgccc cttctggctt
     7501 tctcaggtgt agagatctgc acctccgtat cggctgagga gagttcactc gtggcttcct
     7561 cggtatcttc ttgtatcagt gttgattcct cacctcccgc agcagcatct tgcgacaggg
     7621 aggatgcatt agatgagagc agctccactt tcatgctatg atcatccggg ggagtgcaac
     7681 tggtatcatc gcaggtcatc cactccacta gcacttctac agtcgctttt ggtactgaga
     7741 gtttcacctt ttggacaaaa gtgacactct tctcgtagta gccgatgatc ataccaaaca
     7801 tcggatcatt gaccttgatg ggcttctcaa tagagctgat ttctccaact tttgtcacgc
     7861 ccttagggag cgtgaagatg aaagttgtgg cattaggacc gttttcatag ggtcccatat
     7921 cgtacatgtg ccatccggga gctattaagg ccgttatttt tatgt
//
LOCUS       DAOSIE010000037         7677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_8783_length_7677_cov_4.024403, whole genome shotgun sequence.
ACCESSION   DAOSIE010000037 DAOSIE010000000
VERSION     DAOSIE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8783_length_7677_cov_4.024403"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /gene="miaA"
                     /locus_tag="PLI69_02110"
     CDS             <1..757
                     /gene="miaA"
                     /locus_tag="PLI69_02110"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOG41319.1"
                     /translation="SRVKHHFIHSHSVEQYYTAGKYEIEALALVDELFRSHNTLVMTG
                     GSGLYIDAFCKGLDDFPKVDQELRKSLTARLKREGVESLQQELKTVDPESYDSIDISN
                     GQRVIRALEVTIGTGRKFSSFKTGRTRKREFEILKIGLRRPREVLYNRIDRRVDMMME
                     AGLVEEVRSLVQYRDMPALKTVGYKEIFDYLDGNSSLEEAVRLIKRNTRHYAKRQLTY
                     WGRDEEIKWLEIGSQLPSSDTNLILTSLTSSSV"
     gene            complement(697..2907)
                     /gene="recQ"
                     /locus_tag="PLI69_02115"
     CDS             complement(697..2907)
                     /gene="recQ"
                     /locus_tag="PLI69_02115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOG41320.1"
                     /translation="MKITSEELHAKLKEFFGFNSFKGEQEEIIKHLIAGNDAFVLMPT
                     GGGKSLCYQLPALCLDGTAIVISPLIALMKNQVDAIRGFIHNQEGVAHFLNSSLNKTQ
                     IAEVKEDLLKGITKLLYVAPESLTKEENIQFLKQLKISFYAIDEAHCISEWGHDFRPE
                     YRKIHEIVQEIGEAPLIALTATATPKVQSDIQKNLCMTEARVFKSSFNRPNLYYEIRD
                     KANPKRDIVKFIKENAGKSGIIYCLSRKRVEEIAEFLNVNGIKALPYHAGMDSNTRSY
                     NQDMFLMEEVDVIVATIAFGMGIDKPDVRFVIHYDIPKSLEGYYQETGRAGRDDQEGQ
                     CIAFYSYKDILRLKKFMQGKPISEQEIGNQLLMETVAYAESNQCRRKILLNYFGENYL
                     KEGCGNCDNCLHPKKHFDGQEQMLTVIELILSLKEYFVEEHLANILAGESNSKINSYK
                     HNKHELFGIGKEDGVRFWAAIIRQGVVLQLLDKDIERYGLISVTDKGKAFYESPYPLM
                     LTGARTFTEYDDDDDDEFGGTAGPRVAGSGGGDPELLAILKDVRRSESKRLNLPPWII
                     FTDPSLDDISIQYPITIEELKNCQGVGEGKARKYGKPFLEVIAKYVEENEIERPDDFV
                     VRTVVNRSANKVYIIKCIDRRLPFEDIARARDMDMEELMDEIESIVSSGTRLNIDYYI
                     RQTIDEDKIDDIYNYFREEAVSDSVNDALKELGPAYTEEEVRLVRIKFVSELGN"
     gene            3079..3804
                     /locus_tag="PLI69_02120"
     CDS             3079..3804
                     /locus_tag="PLI69_02120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG41321.1"
                     /translation="MKAIKFCAICVIGAMMMISCSTKTSDEGQPAGITKAQVDSVSYA
                     LGQNFGMVISMNDFGSLNFNSIVSGMKDILAMGKIDPQEANRIGGEFSEVINKFMEDR
                     MRIKGEETLAKGKEFLENNAKNEGVVTTESGLQYKIERAGNGVFPTAESTVECNYEGT
                     LIDGTVFDSSYERGNSATFSLQNVIQGWTEGLQLVDEGGEITLWIPAELGYGERGAGA
                     SIGPNETLKFKVELIRVVPAEEK"
     gene            3950..5776
                     /locus_tag="PLI69_02125"
     CDS             3950..5776
                     /locus_tag="PLI69_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG41322.1"
                     /translation="METFKRILAYAKPWGRYWPGYLALSIFSVIFGIANYALLGPFLT
                     VLFESDTIPQIAAKPEFSFSIQYVNALFSYYLSSIAASDGVVRALLFVSLSLIVACFF
                     SDLCRYLSQRILVSMKTNLMKNVRKDLYNKIASLDIGYFNNRKKGDILSSISNDVNEV
                     QNSVASSFHVMFREPILVVGFLIMLFYMSPRLTAVSLIALPISALIVTRITRNLRRGS
                     VETQSLMGQILSQYEEAISGSRIIKAFNAQKYVENRFERYNERHRKVSRQVSNRQELA
                     SPTSEFLGISIAALVLFYGGWLNFQGNLGMTWPQFIVYIGFYWKVLEPAKAMANSFAA
                     VQKGLVSGDRIFEILDAPSDIIEADYPLPLTSFDHSIEFKNVSFSYTDEPVLKDISFT
                     IPKGKMVAVVGPSGAGKSTMADLLPRFWDVTGGSIELDGIDIRKYKLEDLISQMGIVT
                     QEPILFNDTIANNISFGMENISQEQIEEAAKIANAHDFILQTEEGYQTNIGDRGSKLS
                     GGQRQRIAIARAVLKNPPILILDEATSALDTESERVVQDALSNLMKHRTSLVIAHRLS
                     TIRHADEIIVLQNGVIVERGDHDTLISNHGLYSHLCELQAFT"
     gene            5786..7459
                     /locus_tag="PLI69_02130"
     CDS             5786..7459
                     /locus_tag="PLI69_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG41323.1"
                     /translation="MKKSVNKLFEESFKKNWDYPALSDYKGLTLNYRDVARRIAKLHI
                     FFESCDIVKGDKIAICGRNQSNWGVAFLAALTYGAVPVPLLHEFKPANIHNLVNHSDS
                     KILIVGDSIWENLNSAEMPEVEAILSMNDVSVVYAKDEKYVKAREHLNEMFGKKYPNS
                     FTPANIKYYEDSPNELSMINYTSGTTGFSKGVMLPYRSLHSNILFAKAVHPQLNNTSN
                     LVSMLPTAHMYGMMFEFMFIMSVGGHVHFLTKLPSPNIILDAMATIKPDIIIAVPLII
                     EKIYKKMLSPIISKNRIKLLLNLPVIDQMIQKKILNSIVSAFGGKFKEVIIGGAALNR
                     EAEAFFKRINFPFTVGYGMTECAPIIAYAPWDKTRLYSCGKAAPRMEIKIDSKDPKNI
                     PGEIYVRGDNVFLGYYKNQEATDEVLSNGWLKTGDMGVMDSEGYLFLKGRSKTMILGP
                     SGQNIYPEEIESILNNMPYVVEALVVEDKSLLIALIYPDFDQANEEGLSEEQLLEKLQ
                     EGIQMANIELPNYCKLAGVEIVPEEFEKTPKRSIKRFIYQRNSAPTESN"
     gene            7472..>7677
                     /locus_tag="PLI69_02135"
     CDS             7472..>7677
                     /locus_tag="PLI69_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009435982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41324.1"
                     /translation="MNNKNRNKFDLSYLEMAQVWAKNSYCKRKQVGALIVKDRMIISD
                     GYNGTPSGFENVCEDETGATKPYVL"
BASE COUNT         2167 a         1651 c         1702 g         2157 t
ORIGIN      
        1 ttcccgggta aaacaccatt tcatccactc acactcggta gaacaatatt acactgccgg
       61 aaaatatgaa attgaggctt tggcactggt agatgagctc ttccgaagcc ataatactct
      121 cgttatgaca ggaggttcgg gactctatat agatgctttt tgcaaagggc tcgatgattt
      181 cccgaaagtg gatcaggagt tgcgcaaatc tctgactgca cgcctgaaga gagagggagt
      241 ggagtctctt caacaagagt tgaaaacagt tgaccccgaa tcctacgact ccatagatat
      301 cagcaacgga cagcgtgtga taagagcact ggaggttact attggtaccg gtcgtaaatt
      361 ttcttccttt aagactggca gaacaagaaa aagagagttt gaaattctca aaataggtct
      421 gaggcgtccg cgcgaagtgc tttacaatcg tatagatcgc cgggttgata tgatgatgga
      481 agccggattg gtagaagagg tgcgttccct tgtacaatat cgagatatgc cggcactaaa
      541 gacagtggga tacaaggaaa tatttgacta tttggacgga aatagttctc tggaagaggc
      601 tgtaaggctg ataaaacgca atacccgcca ctacgccaaa cgccagctta catactgggg
      661 tcgtgacgag gagataaagt ggctggaaat cggaagtcag ttgcccagtt ccgatacgaa
      721 cttgattctt acgagtctta cctcttcttc cgtataggca ggacccagct ctttcaaagc
      781 atcattgact gaatcagaaa cggcctcctc tctgaaatag ttatagatat catcaatttt
      841 gtcttcgtca atggtctggc gaatataata atctatgttg agcctggtac ctgagcttac
      901 gattgactca atttcatcca taagctcttc catatccata tccctggcac gagcaatgtc
      961 ttcaaaaggc agacgtctgt caatacactt tattatatat accttattgg cggaacggtt
     1021 gaccaccgta cgtacaacaa aatcatcagg tcgttcgatc tcattctcct ctacatattt
     1081 ggcaataact tccagaaatg gtttaccata tttgcgtgcc tttccttcgc ccactccctg
     1141 acaattcttg agttcctcaa ttgtgatagg gtattggatg gagatgtcat caagggaggg
     1201 atctgtaaat atgatccaag ggggaagatt tagcctcttt gattcacttc tgcgtacatc
     1261 ctttagaatt gcaagaagtt cgggatcgcc tcctccggaa ccggcgaccc taggaccggc
     1321 tgtaccacca aattcgtcat cgtcgtcatc atcgtattcc gtaaatgtcc tcgctcctgt
     1381 gagcatgaga gggtaaggtg actcgtaaaa agctttgccc ttatcggtca cacttatcag
     1441 accataacgc tcaatatcct tgtccaatag ctgcaggact actccctgac gtattatggc
     1501 tgcccagaag cgtactccat cctccttgcc gatgccaaaa agttcatgct tattatgctt
     1561 gtacgaattg atcttggagt tgctctcacc ggcgaggata ttagccagat gctcttctac
     1621 aaaatactcc ttgagggaga ggatgagttc aataacggta agcatctgct cctgaccgtc
     1681 aaaatgtttc ttcggatgga ggcagttgtc gcagttaccg cagccctcct taagataatt
     1741 ttctccaaag tagttgagaa gaatcttgcg ccggcattga ttggattctg cataagcaac
     1801 tgtctccatc agtagctgat ttccaatctc ctgctcggaa attggcttgc cctgcatgaa
     1861 ttttttgagg cgcaggatat ccttgtaact atagaatgct atgcattgac cctcttgatc
     1921 atccctgcct gcacgtcccg tctcctggta atagccctcc aggcttttag gaatgtcgta
     1981 atggatgaca aatctcacat caggcttatc tatccccatt ccaaaagcta tggtagcaac
     2041 tattacatcc acttcctcca tcaggaacat atcttgattg taacttcgtg tattagagtc
     2101 catgccggca tgatagggga gagccttgat accatttaca tttagaaact ccgctatctc
     2161 ttcgacacgt ttacgactca ggcagtagat gataccgctc ttgccggcat tctcttttat
     2221 aaatttgact atatcccgtt ttggattggc cttgtcgcgt atttcataat agagattggg
     2281 gcggttgaat gaggatttga agacgcgagc ttccgtcatg cagagattct tttggatatc
     2341 tgactgaact ttaggagtag cggtagctgt cagcgctatt agcggcgctt cgccgatttc
     2401 ctgtactatt tcgtgaattt tgcggtattc cggtcggaaa tcgtgtcccc attcagaaat
     2461 acagtgggct tcgtctatgg cgtaaaatga gattttcagc tgttttaaaa actgaatgtt
     2521 ctcctctttt gtaagagatt cgggagccac atatagcagt ttggttattc cttttaaaag
     2581 gtcttcctta acctctgcga tttgtgtttt attgagagaa gaattgagga aatgagctac
     2641 cccttcctga ttgtggataa atccgcgtat ggcatccacc tgatttttca taagtgcgat
     2701 aagcggagaa atgacaatgg cagtaccatc cagacacaaa gccggaagct ggtaacagag
     2761 agatttaccg ccacctgtag gcatcagtac aaaagcgtca ttgcctgcta tcagatgctt
     2821 tataatctct tcctgttcac ctttgaacga attgaagccg aaaaattctt tcagttttgc
     2881 gtgcagttct tctgaggtaa tcttcatata taaaggtttt ccttataatt ctactaaatt
     2941 aaacaataat tttcgtattg taaaattttt gtccccccaa aagtgcaata aaagtgctag
     3001 ttgcgaaatt ttcaatatct ttgtgaatta tttttcgtaa aacaatcact aatcaataat
     3061 cataaatatc aaaatagtat gaaagctatc aaattttgcg ctatttgcgt tatcggagca
     3121 atgatgatga tatcttgctc caccaagact tccgacgaag gccagcctgc aggcattaca
     3181 aaggctcaag tggactcagt aagctatgct ctcggccaga acttcggaat ggtaatttca
     3241 atgaatgact ttggttcttt gaatttcaat tctatagttt ccggtatgaa ggatattctt
     3301 gccatgggta aaattgatcc gcaggaagcc aaccgtattg gcggcgagtt ttccgaagtg
     3361 ataaacaagt ttatggagga tcgtatgagg atcaagggtg aggagactct tgctaaaggc
     3421 aaggagttcc tcgagaataa tgccaaaaat gaaggtgtgg taactacaga atcaggcctt
     3481 cagtacaaga tagagagagc cggcaatggt gtattcccca cagctgaaag caccgttgag
     3541 tgcaactacg aaggtactct cattgacggt actgttttcg actcttcata cgaaagaggc
     3601 aattctgcaa cctttagtct tcaaaatgtc attcaaggtt ggaccgaagg tctgcaactg
     3661 gttgatgagg gtggcgaaat taccctctgg atacccgctg aacttggata tggagagcgt
     3721 ggtgcaggtg ccagtatagg ccccaatgag actttgaaat tcaaagttga gctcatccgg
     3781 gtagttccgg cagaagagaa ataaaaagga tattcttaaa agaattgtaa acgggaaggc
     3841 aatgctcttc ccgtttgttt tttggtaagt ttgatttgtt tttggatttg tagttctggt
     3901 atttttttta atatctttgt aagataacac tatttggcat ctgttgtaga tggagacatt
     3961 caagagaata ctggcgtatg cgaaaccatg gggaaggtac tggcccggct atctggcctt
     4021 gtcgatcttc tcggtaatat tcggcattgc aaattatgcc cttctcggtc cctttctgac
     4081 cgtattattc gaatccgata cgataccaca aatagctgca aagcctgagt tttccttttc
     4141 catacagtat gtaaatgctc ttttttcata ctatctctcc tcaatagctg cttcagatgg
     4201 ggtggtcagg gctctgctgt ttgtttctct ttcgctgatc gtagcctgtt tcttctctga
     4261 tttgtgcaga tatctttctc agaggattct ggtaagtatg aaaaccaacc tgatgaagaa
     4321 tgtcagaaaa gacctctata ataagattgc atccctagac ataggctatt ttaacaatag
     4381 aaaaaaagga gacattcttt caagtatatc caatgatgtc aatgaagtgc agaatagcgt
     4441 tgccagcagt tttcacgtga tgttcaggga gcctatacta gtggtaggat ttctaatcat
     4501 gctcttttac atgtcgcctc gtctgacggc agtctcttta atagccctgc cgatatcagc
     4561 gctgatagtt actcgtataa cccgcaatct tcgcagggga tcggtggaga cgcagtcttt
     4621 gatgggacag atcctgagcc aatatgagga ggctatctca gggtcgagga ttattaaagc
     4681 tttcaatgct cagaaatatg tggagaatcg ctttgagcgc tacaatgagc gtcaccgcaa
     4741 agtaagcaga caggtctcta acagacagga gctggcatcg cctacttcgg agtttctcgg
     4801 tatttccata gctgctctgg tactctttta tggcggatgg ctcaatttcc agggtaacct
     4861 tggtatgaca tggccacaat ttatagtcta cataggcttt tattggaagg tgctggagcc
     4921 cgcaaaagcg atggcaaact ccttcgcagc ggtacagaaa gggcttgtct cgggagatag
     4981 gatttttgag attctggatg ctccctccga tataatagaa gctgactatc ctttgccgct
     5041 tacctcattt gaccactcca tagagtttaa aaatgtttca ttcagctata cggatgagcc
     5101 ggtactaaag gatatctcct ttaccatccc taaagggaag atggtggcag tcgtgggtcc
     5161 ctcaggcgcc ggaaaatcca ccatggcaga tctgttgcca aggttttggg atgttacagg
     5221 tggctccatt gagcttgatg gtattgatat ccgaaagtac aagcttgagg atctcatatc
     5281 acagatgggc atcgtaactc aggagcctat tctattcaat gataccatag ccaacaatat
     5341 ctcctttgga atggaaaata tttcgcagga gcagatagag gaggcggcca aaatcgccaa
     5401 tgcgcacgac ttcatattgc aaacggaaga gggctaccaa acaaatattg gagatagggg
     5461 ttccaaactt tccggaggtc agagacagag aatagcaatt gcgcgggccg tgctgaaaaa
     5521 tcctccgatc ctaatactcg atgaggcgac ctccgcactt gacaccgaaa gtgagagagt
     5581 ggtgcaggat gccctttcca acctaatgaa acaccgtact tcacttgtta ttgcgcaccg
     5641 tctttctact attcgccatg ccgatgagat aatagtacta caaaacggag taatagtaga
     5701 gcggggcgat cacgatacat taattagtaa ccatggtctt tactcgcacc tttgcgagct
     5761 gcaggccttt acataatttc aacaaatgaa aaaatctgta aacaaactgt tcgaagagag
     5821 ttttaaaaag aactgggatt accctgcgct ctctgactac aaaggtctta ctctcaacta
     5881 tcgcgacgtg gcacgaagaa tcgcaaaact gcatatattc tttgagagct gcgacatcgt
     5941 caaaggagat aaaattgcaa tttgtggccg caatcagtcc aattggggag tggcttttct
     6001 tgctgctctt acctatggag ccgtacctgt accacttctg cacgaattta aaccggcaaa
     6061 catacataat ctggtcaacc actccgactc aaaaattctt attgtcggag actcaatctg
     6121 ggagaacctc aacagcgcag agatgcctga agtggaggca atactctcaa tgaatgacgt
     6181 cagcgtcgtt tatgcaaagg atgaaaaata cgttaaagcc agggagcatc taaatgagat
     6241 gttcggtaag aaatatccaa atagcttcac cccggctaac atcaagtatt atgaagactc
     6301 tcccaatgag ctctcgatga tcaactatac ctccggtact actggtttct caaagggagt
     6361 aatgcttcct tacaggtctc tccactccaa tatccttttt gcaaaggccg tacatccgca
     6421 actgaacaat acctccaatc ttgtatccat gcttcctacg gcgcacatgt atgggatgat
     6481 gttcgaattt atgtttatta tgtctgtggg aggacatgta cacttcctta ccaagctgcc
     6541 cagccctaat atcatcctgg atgctatggc tactatcaaa ccggacataa tcatagctgt
     6601 tcctctgatc atcgagaaaa tttacaagaa gatgctttct cccatcataa gcaagaatag
     6661 aatcaaacta ttgctgaatc ttcccgtaat agatcagatg atccagaaaa agatactcaa
     6721 tagcatagtc tctgctttcg gtggcaagtt taaagaggtt attattggcg gggccgccct
     6781 taaccgcgaa gccgaggcat ttttcaaaag aatcaacttt ccttttacag ttggttacgg
     6841 catgaccgaa tgtgctccca tcatcgcata cgcaccatgg gacaagacca gactttactc
     6901 ttgtggaaag gctgctccca gaatggaaat taagattgac tctaaagatc ccaaaaatat
     6961 tcccggagaa atatatgtaa ggggcgacaa tgtattcctt ggatattaca aaaatcaaga
     7021 ggcaactgat gaagttctct ccaacggttg gttaaagacc ggtgatatgg gtgtgatgga
     7081 ctcagaggga tatctcttcc ttaaagggcg tagcaaaacc atgattctcg gtccttcggg
     7141 tcagaatatc tatcccgagg agatagagag tatccttaat aacatgccct atgtagtgga
     7201 ggcgcttgta gttgaggata aatccttgtt gattgcgctc atctatcccg actttgatca
     7261 ggcaaacgag gagggacttt cggaagaaca acttctcgaa aagctccagg aaggtatcca
     7321 gatggccaat atagagcttc ctaactactg taaattggcc ggagtagaaa ttgtgcctga
     7381 agaatttgaa aaaacaccaa aacgcagtat caagcgcttc atttaccaga gaaatagtgc
     7441 acctactgaa agcaactgaa ataataatta gatgaataat aaaaatcgaa acaaatttga
     7501 cttgagctat ctcgagatgg ctcaagtttg ggcaaaaaat tcctattgca agcgcaagca
     7561 ggtgggagcc cttattgtaa aggacagaat gataatatca gatgggtata atggcacccc
     7621 ctcaggattt gaaaatgtgt gtgaagatga aaccggtgct accaaacctt atgtcct
//
LOCUS       DAOSIE010000038         7627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_8853_length_7627_cov_3.980058, whole genome shotgun sequence.
ACCESSION   DAOSIE010000038 DAOSIE010000000
VERSION     DAOSIE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8853_length_7627_cov_3.980058"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2599)
                     /locus_tag="PLI69_02140"
     CDS             complement(<1..2599)
                     /locus_tag="PLI69_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOG41325.1"
                     /translation="MKRLLLIALTALICNVAFAQIRVTGKVTSSQDGSPVPFASVVVK
                     GTMQGIATLDDGTYTLDGVPANATLVFSSIGFNDLEMPVSGRNVINAILTPDAEALEE
                     VMVVAYGTVKKGSYSGSATVVEQKTIKDAPIVSFEQVLAGKAPGVQAVSMSGQPGADS
                     QIRIRGYGSFNAGNSPLYVIDGVPATSGNWSSGNASASVMNFLNPSDIESFTILKDAA
                     AASLYGSRASNGVILITTKRGRSGKLTSTFKASAGFSYFAYNHLPLATDAQTEALHRE
                     AWTNYGNANPTRWASYGSLDAYVNAKVAENYPAKDESKYIYKNWEDVIFRTGIIQNYE
                     YSISGGSDKSKIYASVGYTDQQGVVVVSYLKRLSTTINAESQISKWLKIGGNLQYSWQ
                     YQQGHQEGYVTKANPIFMWKIPLSERWPYAYKEDGSLYLECWNPSMTTVNPLGSWGKE
                     YNDYNQNRLLLKAYAEVKFTDWLKAKTIISNDWINQRDRFAWYYGHPNFYAYSKVGGF
                     ISDRNRNISRLVSSTTVNFDKTWGKHHLGAMVGWEAEQETYFYLGLAKIDFSHLGATE
                     SILATGFDNGRSYSQADALLSALSSVNYDYDGKYYLTGTYRRDGSSRLSKETRWGNFW
                     SISGSWRFSNEEWLRADWLNDAKLRASYGTSGTLPSNYYGYMAVYAYTSYGDYGASYP
                     ANLANSDLSWEKNTNWNIGLDATIFDRYTFTAEYYSKLTTDLLLDASVPTTTGFSSTL
                     TNIGSMVNKGWELSVNVDILKTQDWDVSVGANWSYLHNEVLSLSKEGETIVSTPHIWK
                     EGYNFYQFYTRNYLGVSPEDNYMGNSLLKAGYPMYAEGTFYEKGATVNKTVMLKDGTR
                     IEKGGTMPYD"
     gene            2919..6080
                     /locus_tag="PLI69_02145"
     CDS             2919..6080
                     /locus_tag="PLI69_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG41326.1"
                     /translation="MKKILLLALAILTVNVAFAQTRITGKVTSSQDGTPIPFASVIVK
                     GTMNGVATSDNGEYVLENVAPNATLVVSSIGFLNAEIPVGGRTRIDVVLSPDAEALEE
                     VMVVAYGTAKKGTYTGAASVVRQESIKDVPTVSFEQALTGKVAGMQITTTSGQAGSGS
                     AVRIRGIGSMNASNAPLYVVDGVPVVSGSTGQMSDYLYTSNNVMSTLNQSDIESITVL
                     KDAAASALYGSRAANGVILINTKRGKLGRPTVTFKASVGITPSWATDNYETASPQDNV
                     DMLYQIFWDYRTSMGRTPEDASAYSLRQLNSKWNRFGYTFSKPDTERYGNIKIEPLLD
                     GQLKRVDDQGNLIYFDWDDAYFRTAVYQTYDVSVSGGTENSTYYTSFSYTKDQGRIYV
                     NEFDRLSGRVNLNQKVGKHFELATNISVSHTDQSGYNDSRSTTANYFMQTRNLLWPVY
                     WPDDYKTGGPWTARYQSLAQNNIYYNDYWDNSTKTLKLAISETATVKFTDWLNLRTVF
                     SYDDTRVRDHIYYSAQHFSGSSVNGSVSEMMTDYTKLVSSSTLNFAKEFGKHSVSALL
                     GFEAERNWTEYIRATGNDLSTSSLTTVYTAGTKDSNGYAWGNSMMSILSRAEYNYDNR
                     YYASASYRRDGSSRLGASARWGNFWSVAGSWRISNEAFMQDQDVISNLRLRASYGVNG
                     TLPSSNYGWRSLAAYGYPYMTKPGGALANAADANLSWETSYTMNFALEFGLFDNRLNG
                     TVEFFNRDSKDLLQDVPISYVTGFGSTLKNIGSINNHGWEVELGGDIIKNSDFRWSAG
                     LNATFLKSKVVELYDGEDIIWYDPTGDDARAKFIYREGESTLAFWGYEWAGVEKATGL
                     NIWYSNNDNSDTKLNGRNVVYNFSDADEIVIGNAIPFAYGGINTDLEWKGLTFGLNFI
                     YKLGGHLYDGAWKDMADDGYYWNRIRGQLYFENMWTDYNKDGTEPKILGTDPTDSMQK
                     STRHLHNASFLRLKTITLGYRLPNKWISKVGLSSARVYFNGGNLLTFSKYKIADPEVN
                     HYGTRGWETPIGKTYTFGLEISF"
     gene            6102..7562
                     /locus_tag="PLI69_02150"
     CDS             6102..7562
                     /locus_tag="PLI69_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG41327.1"
                     /translation="MKKITIILSLICVLAISSCEGFLDVTPTNQADSSTSITSAADAQ
                     VMINGLMNKMTSSSYYGRNFVLYGDTKGGDLTIYTAGFDDNMYYFAHRAEGGSQIGFW
                     ITGYDCLLQANNIIQGIEALKEEGTTGLDDMLGQALTIRALIHFDLVRLYGKPYNMDG
                     GASHGIPVVTAPVDASDTPQRDPVKKVYDQIVADLTKAAPLISKSKSNGYVNYYANQA
                     ILGKVYMYMDNFSSALTAFKNIIDSKAYTLYTPANWVASWKSQFGSESIFEFAMYPNE
                     GDLGASSLGVRYSRREHAHNSAYGYFLASTYWKNIMGKNDIRWGIMTFDHMRGQEARS
                     DWDDCCYKYLGSPTLAGDGKSTYTAVNIKVIRLSEVYLLAAECALKSDKATAATYLNA
                     ISKRDPDRPAWTAATINEDAVYNEYRRELLTEGKLFFEQMRQNRKVTFEDDAWGFGTT
                     SQYRDKTVDRTFFRAILPIGIDEINSNPDIYQNPGY"
BASE COUNT         2037 a         1747 c         1760 g         2083 t
ORIGIN      
        1 tgtcgtaagg cattgtacca cctttctcaa tcctggtgcc atcctttagc attacagtct
       61 tattgacagt tgcacccttc tcatagaagg ttccctctgc atacataggg taacctgctt
      121 tcagtaacga gttgcccata tagttgtcct caggggaaac tccgagatag tttctggtgt
      181 agaattggta gaagttgtaa ccctccttcc aaatgtgagg tgtgctaact atagtctctc
      241 cctctttgga gagagagagt acttcgttgt ggagatatga ccagttggca cctaccgata
      301 cgtcccaatc ctgagtcttg aggatatcaa cattaacaga gagctcccaa cctttgttta
      361 ccattgagcc gatgttggta agagtgcttg aaaaacctgt ggtcgtaggc actgaagcat
      421 caagcaggag gtcggtggtg agtttactat aatactcagc tgtaaaggta tatctgtcaa
      481 aaatggttgc atcgagaccg atgttccagt tggtgttctt ttcccatgaa agatcggagt
      541 tggccagatt cgcaggatag cttgcgccgt agtcaccata agatgtatag gcatataccg
      601 ccatatagcc ataatagttc gaaggaagtg tgcctgaagt accataggat gcacgcagtt
      661 tggcatcatt cagccagtct gccctcagcc actcttcatt ggagaatctc catgatcctg
      721 atattgacca gaagttaccc cagcgggtct cctttgaaag ccttgaagaa ccgtcacgac
      781 gatatgtacc ggtaaggtag tacttaccat cataatcgta gttcacacta ctcagagctg
      841 aaagcagagc atcagcctgt gagtatgaac gaccgttgtc aaatcctgtg gcgaggatgg
      901 actccgtagc accgaggtgt gagaagtcaa tttttgcaag gccgaggtag aagtaagtct
      961 cctgctctgc ctcccatcct accatagcgc ccagatggtg tttgccccag gttttatcga
     1021 agttgacagt agtggagcta accaggcggc tgatattacg gttgcggtcg gaaatgaatc
     1081 ctcccacttt ggaataggcg tagaagttag ggtgaccata gtaccatgca aagcggtctc
     1141 tctggttgat ccagtcgtta ctgataatgg ttttggcttt caaccagtcg gtaaatttaa
     1201 cttcagcata tgccttgagg aggagacggt tctgattgta gtcgttatat tctttgcccc
     1261 aggatcccaa agggttaacc gtagtcattg aagggttcca acactcgaga tagagtgagc
     1321 cgtcctcttt gtatgcatag ggccatctct cacttagggg tattttccac ataaagatag
     1381 ggttcgcttt tgtaacataa ccctcctggt gcccctgctg atactgccat gagtattgta
     1441 ggttgccacc aatcttgagc catttgctta tctgactctc ggcgttaatg gtagttgaga
     1501 gacgtttgag gtaagagacc acaaccacac cctgttggtc ggtgtaacct accgaagcat
     1561 agattttgga cttgtccgat cctccggaaa tgctgtactc gtagttctga atgataccgg
     1621 tacggaagat aacgtcctcc caattcttat agatatactt gctctcatct ttggcagggt
     1681 agttctctgc aacctttgca tttacatacg catcaaggga accgtaactt gcccatctgg
     1741 tggggttggc gtttccatag ttggtccagg cctccctgtg cagtgcttcc gtttgtgcgt
     1801 ctgttgcaag gggcaggtgg ttgtaagcaa agtaagagaa accggcactt gccttgaaag
     1861 tagaagtaag tttgccggat ctgcctctct ttgtagttat caggattacg ccgtttgaag
     1921 cgcgtgaacc gtaaagtgaa gccgctgccg cgtcctttag aatggtaaaa gactcaatgt
     1981 cgctagggtt caaaaagttc ataacgcttg cagaagcgtt gccggaagac cagttaccac
     2041 tagttgcagg cactccgtct atgacgtata aaggtgagtt gccggcatta aatgaaccat
     2101 agccacggat acgaatctga gagtcggcac cgggctgacc tgacattgaa acggcttgaa
     2161 ctccgggagc tttacctgca aggacttgtt caaagctcac gataggagca tcctttatag
     2221 tcttttgctc gacaaccgta gcagaaccgg aataagatcc cttcttaacg gtaccgtaag
     2281 ctacgaccat aacctcctcc agagcttccg catcaggtgt aaggatcgca ttgattacat
     2341 ttcgcccact tacaggcatc tccagatcgt tgaagccgat ggaggaaaag acaagagtcg
     2401 cattagccgg aaccccatcc aatgtatagg ttccgtcgtc aagagtggct attccctgca
     2461 ttgtgccctt cacgacaaca ctcgcgaagg gaacaggtga gccatcctga gatgaggtca
     2521 cttttcccgt aaccctaatc tgcgcgaacg ccacattgca aatcaatgcg gttagcgcaa
     2581 ttaacaacaa tctcttcata gatttttagt tttgtgtttg gttaattaat atttaagtaa
     2641 ggttgtactt aggttaaggt ttgtagccaa tttcattgtt acacaaaaat aacaaaaaaa
     2701 aaacaaaata aaaagtcctt tttgctgaat tatttcaaac atctaagaaa tatctgaata
     2761 agcgtttgat tttcattttt taaggggcct cgaaaaaaaa tattttgccg ttttaaattt
     2821 taatacctaa ctttatgact tgaattcaga aatttttaac gaatttgaaa aataataaac
     2881 cctaattact taattattaa tacacaaaaa aactatctat gaagaaaatc ttacttcttg
     2941 cactagcgat attgacagtt aatgtcgctt ttgctcaaac aagaattacc ggtaaggtaa
     3001 cttcttctca ggatggtacg ccgatacctt ttgcaagcgt tattgtaaaa ggtacaatga
     3061 atggtgttgc cacttctgac aatggtgagt atgtattgga gaatgttgct ccaaatgcta
     3121 ctctcgttgt ttcttctatc ggttttctga atgccgaaat cccggtggga ggcagaacca
     3181 ggatcgatgt agtccttagt ccggatgccg aggccctcga agaggtgatg gtcgttgcct
     3241 atggtacggc caaaaaaggt acctataccg gagctgcttc cgtagtgaga caggagtcca
     3301 tcaaggatgt gcctacggtt tcatttgaac aggccctaac cggaaaggtt gcaggtatgc
     3361 agattaccac aacttcagga caggccggtt ccggttctgc agttcgcatt cgcggtatcg
     3421 gttccatgaa tgcttccaac gctcctcttt atgtggttga cggcgtacca gttgtttcag
     3481 ggagtacagg tcagatgagt gattacctct acacctccaa caacgtgatg tcaacactta
     3541 accagagtga tatcgaatcc atcacagtcc ttaaggatgc tgccgcatcg gcactttatg
     3601 gttcgcgtgc cgcaaatggt gttatcctta ttaataccaa gagaggtaag ctgggacgtc
     3661 ctacggtaac cttcaaagca tccgtgggta taactcctag ctgggctacc gacaactatg
     3721 agactgcttc tcctcaggac aacgtggata tgctttatca gattttctgg gattatcgta
     3781 catccatggg cagaactccg gaggatgcaa gtgcatattc gctacgccag cttaacagca
     3841 aatggaatag attcggttat acatttagca aaccggatac cgagcgttac ggcaacatta
     3901 aaatcgagcc cctcttggac gggcagctca aacgtgttga cgaccagggc aacctgatct
     3961 attttgactg ggatgatgcc tatttccgca cggcagtata tcagacttac gacgtaagtg
     4021 ttagcggtgg taccgaaaac agcacatatt atacttcatt ctcctataca aaggaccagg
     4081 gacgtatata tgtaaatgag tttgaccgtc tcagcggtcg tgtaaatctc aatcagaagg
     4141 taggtaagca tttcgaactg gcaaccaata tcagcgtgtc acacacagac caaagtggtt
     4201 ataacgactc gagaagcacg actgccaact acttcatgca gacccgtaac cttttgtggc
     4261 cggtttattg gccggatgac tataagacag gcggaccatg gactgcacgt tatcagtcac
     4321 ttgcccagaa taatatatac tataacgact actgggacaa ttctacaaag actctgaaac
     4381 ttgcaatcag cgagacagct acggttaaat ttactgactg gctcaactta cgcacagtat
     4441 tttcatacga tgataccaga gttagagacc atatctatta tagcgcacag cacttcagcg
     4501 gctcttctgt aaatggttcg gtttccgaaa tgatgactga ctacactaag ctcgtttcat
     4561 catccactct gaactttgca aaagagtttg gcaaacacag tgttagcgca cttctcggct
     4621 ttgaggcgga gcgcaactgg accgagtata ttcgcgctac tggtaatgac ttatccacca
     4681 gttctcttac tactgtttat acagcaggta ccaaggactc caacggatat gcttggggta
     4741 actcaatgat gtccattctt tcgcgtgccg aatacaacta cgataacaga tactatgcct
     4801 ctgcttcata ccgccgcgat ggttcctcaa gactcggtgc ttctgctcgt tggggtaact
     4861 tctggtcagt tgccggttct tggcgcatca gtaacgaggc ctttatgcag gaccaggatg
     4921 tgatcagcaa ccttcgctta agggcttctt atggcgtcaa cggtacactt ccttcaagta
     4981 actacggctg gcgttctctc gctgcttatg gttatcctta tatgacaaag cccggtggag
     5041 cacttgcaaa tgctgctgac gccaaccttt cctgggagac cagttacacc atgaactttg
     5101 cattggagtt cggtctgttt gacaacagat tgaatggtac tgtagagttc ttcaatcgtg
     5161 actccaaaga cctgctgcag gatgttccca tctcttacgt aacaggcttc ggctctaccc
     5221 tcaagaatat cggttcaatc aacaaccacg gctgggaagt ggaacttggc ggcgacatca
     5281 tcaaaaattc agatttcagg tggagtgcag gccttaatgc cacattccta aaatcaaaag
     5341 tggttgagct ctatgatggc gaagatatca tctggtacga tcctaccgga gatgatgcac
     5401 gtgcaaaatt catctatcgc gaaggtgagt ccactctcgc attctgggga tatgaatggg
     5461 caggtgttga gaaggctaca ggtctcaata tctggtattc aaataatgat aattccgata
     5521 cgaagcttaa cggtcgcaac gtggtttata atttctccga tgcagacgag atcgtaatag
     5581 gtaatgctat tcctttcgcc tatggcggta tcaataccga tctggaatgg aagggtttga
     5641 catttggcct gaatttcatc tacaaactcg gtgggcatct ctatgacggt gcttggaaag
     5701 acatggctga tgacggttac tactggaacc gtatccgtgg ccagctctat ttcgaaaata
     5761 tgtggaccga ttacaacaaa gatggtacgg agcctaagat tctcggtact gaccctactg
     5821 attctatgca aaagagtacc cgtcacctcc acaatgcttc gtttctcaga cttaagacta
     5881 ttacgctggg ctacagactc cctaacaagt ggatcagcaa agttggcttg agcagtgcgc
     5941 gcgtttattt caacggtggc aaccttctta ccttctccaa gtacaagatt gctgaccctg
     6001 aggtcaacca ttacggtacg agaggttggg agacacctat cggaaagacc tacacattcg
     6061 gtttggaaat cagtttctaa tgaaaataat tgtttaagaa tatgaaaaaa ataactatca
     6121 tactttcatt gatctgcgtc ctggcgattt cttcctgcga aggctttttg gatgtgaccc
     6181 ccaccaacca ggcagactct tccaccagca taactagtgc tgctgacgcc caggtaatga
     6241 tcaacggtct aatgaacaag atgacctctt ccagttatta cggccgtaac tttgtactct
     6301 atggtgatac caaggggggt gaccttacca tctatactgc cggttttgac gacaacatgt
     6361 actactttgc ccacagggct gaaggtggtt cacagattgg tttctggatc acgggctacg
     6421 actgccttct ccaggccaac aatattatcc aaggtattga ggccctcaaa gaggagggta
     6481 ctaccggtct tgacgatatg ctcggacagg ctctcactat tcgtgcactg atccatttcg
     6541 acctggttcg tctctatggc aaaccttaca atatggatgg tggtgcgtcc catggtattc
     6601 ccgttgtgac agctccggtt gatgccagcg atacacctca gcgagatcct gtaaagaagg
     6661 tgtatgacca gatagttgcc gaccttacta aagcagctcc gctcatttca aagagtaagt
     6721 caaatggtta tgtcaactac tacgccaacc aggcaatact tggcaaggtt tacatgtata
     6781 tggataactt ctcatcagca ttaactgcct tcaagaatat catagacagt aaggcctata
     6841 ccctctacac acctgcaaat tgggtggctt cctggaaatc acagttcggc agcgagtcca
     6901 tctttgaatt tgcaatgtat cccaatgagg gtgaccttgg tgccagctcc cttggagtac
     6961 gctacagtcg cagggaacat gcgcacaata gtgcatatgg ctatttccta gccagtacat
     7021 actggaaaaa cataatgggt aagaatgata tccgttgggg tatcatgact tttgaccaca
     7081 tgaggggcca agaggcacgt agcgactggg atgactgctg ctacaagtat ctgggttctc
     7141 ctactctggc cggtgacggc aagagtacgt acacagcagt aaacatcaag gttatccgtc
     7201 tatccgaagt ttacctgctt gccgctgaat gtgctctcaa gagtgacaaa gccactgctg
     7261 caacctacct caatgctatt tcaaagcgtg accccgacag acctgcctgg acagctgcaa
     7321 ccatcaacga ggatgctgtt tacaatgagt atagaagaga gctccttaca gagggtaagc
     7381 tcttcttcga gcagatgcgt cagaacagga aggtgacttt tgaggatgac gcatggggtt
     7441 tcggtactac ttcacagtat cgtgacaaga ctgttgacag gacattcttt agggctattc
     7501 ttcctatcgg tatcgatgag attaactcta accctgacat ttatcagaat cccggttatt
     7561 aataatcgtg attaggtata attcctgaat tgaaaaaagc cggttttgta ccggcttttt
     7621 ttaatga
//
LOCUS       DAOSIE010000039         7580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_8925_length_7580_cov_3.901130, whole genome shotgun sequence.
ACCESSION   DAOSIE010000039 DAOSIE010000000
VERSION     DAOSIE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8925_length_7580_cov_3.901130"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="PLI69_02155"
     CDS             complement(<1..136)
                     /locus_tag="PLI69_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOG41328.1"
                     /translation="MGENLVIVESPAKAKTIEKFLGKGYTVKSSFGHIRDLSEKGLSI
                     D"
     gene            237..2609
                     /locus_tag="PLI69_02160"
     CDS             237..2609
                     /locus_tag="PLI69_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOG41329.1"
                     /translation="MPNNKKSVDRKRLLKWFWWIIITPVAILILFVAGVAFFAHIPSF
                     EELEDPKSNLATELISEDGKVINTYHIENRSYVSFADLPPHLVNAAISTEDARFYRHS
                     GIDFRGLVRVVVKTLLMGDSSQGGGSTITQQLAKTLYPRQDVSSRIPGGKFIKLVTIK
                     IKEWITAVKLERNYTKDEIVTMYLNAIFFGSNAYGIKSAALTFFDKDVSDLTVEEGAC
                     LVGMVNKPTRYNPVINPENSLSRRNHVLRQMEKYDYISPEQYDSIVQIPIILSYQQQD
                     HNAGLGSYYRDMLRRYMNAEEPRPSSYRQKEDYVADSTLWMEDPLFGWLNKNPKPDGT
                     QYNLDKDGLRIYTTIDSRMQQYAEDAVREHLGKDLQKVFFNELRRKPNAPFSPDVTPE
                     VTQMLMNQAVKWSDRYRQMKKEEVSEEEILKSFKQKVQMRLFAWNEQGYIDTLMTPYD
                     SIRYYKSFLRAAFIAIEPHTGKVKAYIGGPDYRYFKYDNARQGKRQVGSTIKPFLYTL
                     AMQEGFTPCDKVVNIPYSFPVGDEVWTPKSTDRAEHIGKTVTLKWGLTHSSNNISAYL
                     MKQFGPHALVEMCRKMGISSYLDPVYSLCVGSCDISVYEMVAAFNTFPSRGVYVAPML
                     VNRIEDKSGNVLANFSSRKKEALSDPTAYLMVNLMQGVVNEGTAGRLRWKYGLGNNIA
                     GKTGTTNDQSDGWFIGFTPKLTAGVWVGAEDRQVHFESLTYGAGSNMALPIWGIFMQK
                     VYGDESINIGTEDLFVPPLGWSMDLSCTGDDSDLSNDKISTSGDDQGFFN"
     gene            2625..3635
                     /locus_tag="PLI69_02165"
     CDS             2625..3635
                     /locus_tag="PLI69_02165"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOG41330.1"
                     /translation="MTKRNLALIYGGDSAEVGISILSGRNVSRYIDRSKYNVYHVLLK
                     GASWQVVEASGEATESNPEAGLTGLAQVDKNDFSFTYRGEKIKFDLAMIMIHGTPGEN
                     GVLQGYFEMMGVPFTTSSSAVSASAFDKQICKALLRDFGFKLAPDVTLRRGKGFSVTS
                     IVDKLGLPLFVKPCSGGSSFGVSKVMKEEELEGAIMLAFRHSDTVLVETYISGREITQ
                     GAYMDGEKIVALPVTEIIPHNSFFDYEAKYNGLSDEICPADIDPIKVEEISATTRSIY
                     DCLGCKGIVRVDYFLCADGSVVFLELNTVPGMTPMSLVPQQIRTAGLDMMQVITGIAD
                     NT"
     gene            3653..4522
                     /locus_tag="PLI69_02170"
     CDS             3653..4522
                     /locus_tag="PLI69_02170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HOG41331.1"
                     /translation="MTLKGFVDKSVKDLSALYPEAEAKSMVIRLLSHYLELPEYCCIS
                     DPDRKISAAEQQKLDGAMGDLLKWRPLQYVLGFTDFGGRRFKVIEGVLIPRPETEELF
                     GIIVDDLSERELDEEQDFNILDVGTGSGCLAWSLSAELAGAQVFGCDISDAALRVAGK
                     QKVKSTEGPVKRPVFFWADMLKPPPAGLPQFDVIVSNPPYVLESERPQMRLNVLGYEP
                     EPALFVPDDDPLLFYRALLSWTKLLLRERGICYFEINERFGSEVADLFGPGTTVLKDI
                     NGRDRFVKHVNKA"
     gene            4582..5736
                     /gene="nagA"
                     /locus_tag="PLI69_02175"
     CDS             4582..5736
                     /gene="nagA"
                     /locus_tag="PLI69_02175"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOG41332.1"
                     /translation="MLTQIINGIIHTPSGWLKEGSIIIGDNKILEVSNCDLPVVGAKI
                     IDAKGMYITPGAIELHFHGGGGSDFMEGTEEAFRRAIATHMQYGTTSMYPTLSSSSIP
                     MIRAAVATTEKLMAEKNSPILGLHLEGHYLNVKMAGGQMPQYIKDPDPGEYIPILEET
                     DCIKRWDIAPELPGATAFGRYITRKGVLASIAHTAAEYHQVLEAYEAGFTHVTHFYNA
                     MPGFHRKNEYKYEGTVESVYLIEDMTVEVIGDGIHVPATILELIYKIKGVEKTALITD
                     ALACAASDNPAAFDPRVIIEDGVCKLSDRSALAGSIVTMDRLIKTVATQTSIPFFDVI
                     RMVSESPARIMGVMDRKGSLENGKDADILFIDKNYDLRGVIAMGQIIKSI"
     gene            5761..6906
                     /locus_tag="PLI69_02180"
     CDS             5761..6906
                     /locus_tag="PLI69_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG41333.1"
                     /translation="MKLIQIFNGKILTPQGLINHGSLLIKEGRILAITGSSLPLENAE
                     MFDASGRYVIPGYVAMNVYGAAGSQFHDATEEAFNTVINTHLTHGTTTIFPTVGAVGY
                     DKLLKTGKICTKLMERGNRLIGGLHIVGPYLSPEITKGRYEVKLPDRSKYEKVVSELP
                     CIKRWDAAPELPGAQEFARFLTRKGILAAISHTMAEYGDVKGGFDAGFLHAAQLYNAM
                     AGFHKRRKYKYEGTVESVLLIDEMTTEIIADLVHLPATILRLVYKLKGVERTALVTAA
                     LRYAAYDGEELPFSKVYIENGICKLKSDGTLAGGISTMDQLVKNMVIRAEVPFEDAVR
                     MASETPARIMNVIDRVGTLEKGKDADILLLDHQYNLTSVWSKGVKIF"
     gene            complement(6893..>7580)
                     /locus_tag="PLI69_02185"
     CDS             complement(6893..>7580)
                     /locus_tag="PLI69_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41334.1"
                     /translation="IEEIVGEYRLNRYYDHYKKDILVACAAAIKYNIAVGAEAAEAYA
                     KAGLINPNPRDPFRIAAKDLFASIPLTIQEDMILFPATVRKQVPKILEEIIRAKGEPM
                     TAEEILKEYNRLYPGEKKRKKFVSGNVLRSPSITAIGRTGRYTLKEWKKGSRRGGTIR
                     LFVREYLNTQPGKSAPISDVVDYVLQFRPTTNRSSIISNLQLDRRGSFLFSEQEGVRY
                     LTLKPSTKKS"
BASE COUNT         2045 a         1664 c         1840 g         2031 t
ORIGIN      
        1 tgtcaatgct gaggcccttt tcactcagat cccggatatg gccgaagctc gactttacag
       61 tataaccttt tcccagaaat ttctcaatgg tctttgcctt ggcaggcgac tcaactatta
      121 ccagattctc tcccatattc cttattcttt tcaaactgaa atgcaaagta agggataaag
      181 aaagcaatta tccaaatttt aattgttatt tttgcgcaat caaaacattt tgatctatgc
      241 caaataataa gaaatcggtt gatcgaaaga ggttgttgaa gtggttttgg tggataatta
      301 ttacacctgt tgcgattctc atcctctttg tcgccggagt agcttttttt gcacatattc
      361 cctcatttga ggagcttgag gaccctaaga gcaatcttgc aacggaactg atttccgaag
      421 atggcaaggt catcaatacc tatcacattg aaaatcgttc atacgtctcc tttgcggact
      481 taccccctca tctggtcaat gcggctatct ccacggaaga tgcgcgcttt taccgtcatt
      541 cgggcataga cttcagaggg ctcgtccggg tcgtggtaaa gactctgctc atgggagatt
      601 ccagccaggg cggtggcagc accattactc agcagcttgc aaagaccctc tatccgcgtc
      661 aggatgtttc cagccgaatt cccggaggca aatttatcaa acttgtcacc attaagatca
      721 aggagtggat taccgcagtc aagctggagc gcaattacac aaaggatgag attgtaacga
      781 tgtatctcaa tgcgattttt ttcggaagta atgcctacgg catcaaatcg gcagctctca
      841 cattctttga caaagatgta agcgacctaa cggtcgaaga gggggcttgt ctggtgggaa
      901 tggtcaataa acccacccgg tacaatcccg tcattaatcc tgagaattct ttgagtcgtc
      961 gcaaccatgt actgaggcag atggaaaaat acgattacat ctccccggaa caatatgact
     1021 ccatagtaca gatacctata atactctcat accaacagca ggatcacaat gcgggccttg
     1081 gttcatacta tagagatatg cttcgccgct acatgaatgc agaggagccc agaccctcct
     1141 cttacaggca gaaggaggac tatgtggctg attccaccct gtggatggaa gatccgcttt
     1201 ttggctggct caacaaaaat ccaaagccgg acggcacgca gtacaatctt gataaggatg
     1261 gactccgtat ctatactaca atcgattcca gaatgcagca atatgcagaa gatgccgtaa
     1321 gagagcattt gggtaaggat cttcagaagg tcttcttcaa tgagctcagg cgtaagccca
     1381 atgccccttt ctcccccgac gtaactcctg aagttactca aatgctgatg aatcaggccg
     1441 taaaatggag tgatcgatac cgtcagatga aaaaggagga ggtgagtgag gaggaaatcc
     1501 taaaatcctt taaacagaag gtacaaatgc gtttgttcgc ctggaatgag cagggttaca
     1561 tcgatacatt gatgacacca tacgattcca tcagatatta caaatcattc cttcgtgctg
     1621 catttattgc gatagaaccg cataccggta aagttaaggc ctatattggc ggccccgatt
     1681 accgttattt caaatatgac aatgcgagac agggcaaacg acaggtagga tccaccatca
     1741 aacctttcct ctacactctt gcaatgcagg aaggtttcac gccttgcgac aaggtggtca
     1801 atattcccta ttcattccct gtaggagatg aagtatggac ccccaagagt accgacagag
     1861 cggaacacat aggcaagacg gttacactca aatggggttt gactcacagc tccaataata
     1921 tctcagctta tctgatgaag cagttcggtc ctcatgcttt ggtggaaatg tgccgtaaga
     1981 tgggtatcag ttcatactta gatcccgttt attcgctatg cgtgggctct tgtgatattt
     2041 ccgtttacga aatggttgct gctttcaata ccttcccaag ccgtggcgtc tatgtagccc
     2101 cgatgctggt caatcgtatt gaagataagt ccggtaatgt gctggcaaac ttttcctccc
     2161 gcaaaaaaga ggcattgagt gaccctaccg cctatctgat ggtcaatctc atgcagggtg
     2221 tggtcaatga gggtactgca ggacgccttc gttggaagta tggtttgggc aacaacattg
     2281 ccggcaagac cggtactacc aatgatcaat cagacggatg gttcataggc tttaccccga
     2341 aactgactgc cggagtttgg gtaggtgcag aggatcgcca ggtacacttc gaaagtttga
     2401 cttatggtgc cggatccaat atggcgcttc ccatatgggg catatttatg cagaaagtat
     2461 atggagacga aagcatcaat atcggtactg aggacctctt tgtacctcct ttgggttgga
     2521 gcatggactt gtcctgtacc ggagatgata gtgacctttc aaacgataaa atatcgacat
     2581 ccggagacga tcaaggtttt ttcaactgat aactcactca ttttatgaca aagcgcaacc
     2641 ttgcactcat atatggcgga gactccgcag aggtaggaat ttccattctc agcggtcgta
     2701 acgtctcccg ctatatcgac cgttccaaat acaatgttta tcacgtactc cttaaagggg
     2761 cctcatggca ggttgtggaa gcctcaggag aggccactga atccaatccg gaggcaggtt
     2821 tgacagggct ggctcaggtg gataaaaatg acttttcttt tacatatcga ggagaaaaga
     2881 taaagtttga tctggcgatg atcatgatcc atggtactcc aggagaaaac ggagtcctac
     2941 aaggctattt tgagatgatg ggagtacctt ttactacctc ctcatctgcg gtctcggctt
     3001 cggcattcga caaacagata tgtaaagccc tgttgcggga tttcggattc aaattggctc
     3061 cggacgttac tttaaggaga ggtaaagggt tttcggtgac aagtattgtg gataaattgg
     3121 gactacccct ctttgtcaaa ccatgttcag ggggcagcag ttttggagta agcaaggtta
     3181 tgaaggagga agagttggag ggggcaataa tgctcgcatt cagacatagt gacacggtac
     3241 tggtggaaac ctatatcagt ggacgtgaga taacgcaggg agcctacatg gatggagaga
     3301 agattgtagc attgcccgtg actgagatta taccccacaa ctcctttttt gattatgagg
     3361 ctaaatacaa cggcttgagt gacgaaatat gcccagcaga catagatccc ataaaggttg
     3421 aagagatttc ggctactaca cgcagcatat atgattgttt gggttgtaaa ggcatagtca
     3481 gggtggacta tttcctttgc gctgacggca gtgtggtatt cctcgaactc aacacagtac
     3541 ccggaatgac tcccatgagc ctggtacccc agcaaatacg tacagccgga ttggatatga
     3601 tgcaggtaat aaccggtatc gcagataata cctgatcatc tctttttaaa atatgactct
     3661 taaaggattt gttgacaaat cggtaaagga tctctcggca ctctacccgg aagctgaagc
     3721 caagtcaatg gttatcaggc tactatctca ctatcttgag ttgcccgaat attgttgtat
     3781 atcggatccc gacaggaaga tttccgctgc cgaacagcag aagctggatg gagctatggg
     3841 ggatttgcta aaatggcgac ctcttcagta tgtgctcggt tttaccgatt tcggagggag
     3901 acggttcaag gtaattgagg gggtactgat tccccgtcca gagaccgaag agttatttgg
     3961 catcattgtc gatgatctgt cggaaaggga gctcgatgag gagcaggatt tcaatattct
     4021 cgatgtcggt accgggtcgg gatgcctcgc ctggtcgctt tcggcggaat tggccggagc
     4081 gcaggtgttt ggttgtgaca tatcggacgc agcccttagg gtagcgggga aacaaaaagt
     4141 caagagtacg gagggtccgg tcaaaaggcc tgttttcttt tgggcagata tgttgaaacc
     4201 acctcctgca ggtcttccgc agtttgatgt aattgtatca aatccccctt atgttcttga
     4261 gagcgagcga ccgcagatgc gtctcaatgt cctcggttac gagcctgaac cggccctttt
     4321 tgttccggat gatgatccac tgcttttcta tagggcgctg ctctcctgga caaaattgct
     4381 tttacgggag agaggtattt gttattttga gataaatgag cgtttcggta gtgaagtagc
     4441 tgatcttttc ggccccggca cgacagttct taaggatatc aatggtcgcg accgctttgt
     4501 caagcatgtt aataaagcgt agcataattt ttgttactat ctttataaat tgtttcaaaa
     4561 ataaatttaa aagaatatat gatacttact caaataataa atggtattat acatacccct
     4621 tcaggatggc ttaaagaggg ttccatcata ataggtgata ataagattct cgaagtttca
     4681 aattgcgatc tgcctgttgt aggtgccaaa ataatagatg caaaaggtat gtacatcacg
     4741 ccgggagcaa ttgaactcca ctttcacggc ggtggcggca gtgactttat ggaaggcacg
     4801 gaagaggctt tcaggagggc aattgccaca cacatgcagt atggtactac cagtatgtac
     4861 cctacacttt catcctcgag cattccaatg attagggctg ccgtagcaac tacggaaaaa
     4921 ttgatggcag agaaaaacag tccgattttg ggactccatc tagagggaca ttacctcaat
     4981 gtcaaaatgg caggtggaca gatgccgcaa tatatcaagg atcccgaccc cggcgagtat
     5041 attcccatac tggaagagac cgactgcatc aagcgttggg acatagcgcc ggaacttccc
     5101 ggagccactg catttggacg atacattacc agaaagggcg tgctggcctc tattgctcat
     5161 actgctgccg aataccacca ggtactggag gcatacgaag ccggctttac tcatgtcact
     5221 catttctaca atgcgatgcc cggttttcat agaaaaaatg aatataagta cgaagggact
     5281 gtagagagcg tttacctcat tgaggatatg actgttgagg taattggtga cggtatacac
     5341 gtacctgcca ccatactgga gcttatctat aagattaagg gggtggagaa gacggcactt
     5401 attaccgatg ctcttgcctg tgctgccagt gacaatcctg cggccttcga tcctcgtgta
     5461 atcatcgaag atggagtctg caaactttcg gatcgttctg ccctggccgg ttctatagtc
     5521 acgatggacc gcttgataaa aacggtagcc actcaaactt cgatcccttt tttcgatgta
     5581 atcagaatgg tttccgaatc tccagcccgt ataatgggtg ttatggatcg taaagggtcc
     5641 ctcgaaaatg gcaaggatgc ggatatattg ttcattgata agaactatga cctccgtgga
     5701 gtgattgcca tggggcaaat catcaaatct atctaagata acatttataa ttaccgaact
     5761 atgaagttga tacagatatt caatggaaag atcctgaccc ctcaagggtt gatcaatcac
     5821 ggctccctac tgatcaaaga aggtcgtatc ctcgccatta ccggcagctc cctccctttg
     5881 gaaaatgcgg aaatgttcga tgcttcggga cgctatgtaa taccgggata tgttgcgatg
     5941 aatgtatacg gagcagcagg cagtcagttt catgatgcta ctgaggaggc attcaatacc
     6001 gtaatcaata cccacttaac tcacggtacc accaccatat tccctactgt tggtgcggtt
     6061 ggctacgata agctgctcaa aaccggaaaa atatgcacta aattgatgga aaggggtaac
     6121 cgcctcattg gaggtctgca tattgttggc ccctatctga gccctgaaat aacaaaaggc
     6181 aggtatgagg taaaactgcc ggatcgatca aagtatgaaa aagttgtgag cgaacttccc
     6241 tgcataaagc gttgggatgc tgctcccgaa ctccccggag cgcaagaatt tgcccgtttt
     6301 ctgacacgta aaggcatctt ggcggccatc tctcacacca tggcggaata tggcgatgtt
     6361 aaagggggat ttgatgccgg atttctccat gcggcacagt tatacaatgc tatggcagga
     6421 tttcacaaac gccgtaagta caaatatgaa ggcacagttg agagtgtttt gttgatagat
     6481 gagatgacta ctgagattat tgcggatctg gtccatttac ccgcgaccat tttacgtctt
     6541 gtttataaac tcaaaggtgt ggagaggact gcactggtta ccgctgcact ccgttatgct
     6601 gcttacgatg gtgaggaact tcctttctcg aaagtctaca tagaaaatgg gatatgcaaa
     6661 ctcaaatcag acggtactct agccggggga atctctacaa tggatcagct agtcaaaaat
     6721 atggtcataa gagctgaggt gccttttgag gatgccgtta ggatggcgtc ggagactcct
     6781 gcaaggataa tgaatgtcat agatcgcgtt ggtaccctcg aaaagggtaa ggatgccgat
     6841 atcctactgc tcgaccatca atacaacctg acctccgtat ggagtaaggg tgttaagatt
     6901 ttttagtaga aggtttaaga gtcaaatatc tgactccttc ctgttcggag aagaggaagg
     6961 aacctctgcg gtcaagctga aggtttgata ttattgaact tctgtttgtt gtaggtctga
     7021 attgcaatac ataatccacg acatccgaaa tgggagcact ctttccgggt tgcgtgttca
     7081 ggtactccct gacgaaaaga cgtattgtac cgcctctgcg agaccccttt ttccactctt
     7141 tgagagtata gcggccggta cgtccaatgg cagttattga gggagaacgg agtacatttc
     7201 ccgaaacaaa cttcttcctc tttttttcac ctggatatag gcgattgtat tccttcagta
     7261 tctcctctgc ggtcatcggc tccccctttg cacgtattat ctcttccaat attttcggga
     7321 cttgctttct tactgtagcg gggaatagga tcatatcctc ctgaatggtt aggggaattg
     7381 aggcaaaaag atccttggct gctatacgga agggatcccg ggggttagga ttgatcagtc
     7441 ctgccttggc ataggcttcc gctgcttcgg ctcccactgc tatattatac tttatggcgg
     7501 ctgcgcaagc gaccagaata tcctttttat agtggtcgta ataacggttt aatcggtatt
     7561 cgccgacaat ttcctcaatg
//
LOCUS       DAOSIE010000040         7500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9032_length_7500_cov_2.786702, whole genome shotgun sequence.
ACCESSION   DAOSIE010000040 DAOSIE010000000
VERSION     DAOSIE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9032_length_7500_cov_2.786702"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="PLI69_02190"
     CDS             <1..1535
                     /locus_tag="PLI69_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOG41335.1"
                     /translation="ASQVEKRSEKSVEERLSDAMVSGDTLYLEEDIRQALDKYSPIEI
                     VEGALLEGMERVGLLFSEGKMFLPQVIKSAKVMRNAVAILQPLMESDQVDIPRLRRKM
                     ILATAKGDVHDIGKNILGIVLSCNNIEIIDLGVMVDNETIIEAIHSHKPDFVGVSGLI
                     TPSLQYMEQLCRMMEKEALELPLFIGGATTSALHTAVKLAPLRTSAVIHTSGASDCAN
                     MINRLSREPQKTLEEVKASQEQLRNLYHQSNETFVSLEEARARVEKYPLESYQLPEAF
                     GEDNLHVRFLPVETLVDKIDWNMLLAFWGFKGGYPAIVYSNPEAAQCYESAIAAIDSM
                     IVDRSVDISMEVRFFDAQSSGETIILDGKHRFEMPRSTSERSDYRSLADFVVPEGSGL
                     VSTVGLFAIKVEDLQRCDDCRDYEHLLRESICARLAEAAAEWMEEQVSEGTVLIRPAF
                     GYAACPDHSQKNLACRLLDASERIGVQLSETSALIPSTSLCGMLISHPKAHYPIIRNK
                     KE"
     gene            1541..2494
                     /gene="metF"
                     /locus_tag="PLI69_02195"
     CDS             1541..2494
                     /gene="metF"
                     /locus_tag="PLI69_02195"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOG41336.1"
                     /translation="MKVIDIINSKSGPFPSLEFVPPLKGSDINRLYSSIEPFMEFNPP
                     FINITCHREEIEYRENADGTISRYVLTKRPGTVAIAAAVMKRFPIEVVPHIICSGTNR
                     FAIENELIDLNFLDIQNVMALRGDAPAGHKKFKAEPDGCRYSNELVAQIKAMNEGRYI
                     DDSIKDAVPTNFCIGVAGYPEKHFEAPNLEVDIENLKRKVDAGADYVITQMFFDNNKY
                     FEFVAKCREKGIKVPIIPGLKPLSTAAHLTKLPEAFSIDIPQELVSLVKECKSEDQIY
                     QLGIEWCVSQSKDLIAKGAPAIHYYTMGRGNNVTEVLKRVF"
     gene            complement(2558..2830)
                     /locus_tag="PLI69_02200"
     CDS             complement(2558..2830)
                     /locus_tag="PLI69_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOG41337.1"
                     /translation="MMIFIYGLSYKARREHLIQLFEQYGTVTSARIIVDPETKRSRGY
                     GFVEMNDEEGAAAIAALNGSKHMNRPIHVEVAVNPPKKRSEKKDNE"
     gene            complement(2882..4291)
                     /locus_tag="PLI69_02205"
     CDS             complement(2882..4291)
                     /locus_tag="PLI69_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOG41338.1"
                     /translation="MGGLFGVISKRECKTELFYGTDYHSHLGTKRAGMAVLNRDGLFA
                     RSIHDIQNSYFRIKFESDLHKFDGNSGIGVISDTDAQPILINSRLGRFAIVTVGKINN
                     VEELEEEFLAKGKNFAEISSGKTNQTELVSHLVTEKNSFAEGIEYAQNRIRGSLTMLI
                     LTSEGIIAARDKYGRTPLIIGRNEDSMAVASEDCSFPNIGFESEYILGPGETVLLTAD
                     GITQLLAPQKELQICSFLWIYYGYPTSNYEGINVDDVRNRLGSIMGAKDDTPLDVVSS
                     IPDSGTCMAVGYARGKGIPFRAAMVKYNTTWPRSFTPVSQSTRELVAKMKLIPNYSII
                     KNKRVAFCDDSIVRGTQLKNNVKALYNHGAQEVHIRISCPPIIYPCPYLNFSSSRSSL
                     ELITRKFIDEVDKAGPADLHKYSCESCPEHARMVEYIRKKLNMSTLCFNSVKEIVQAI
                     GLPKESLCTHCFDGTSYGQ"
     gene            complement(4344..5387)
                     /locus_tag="PLI69_02210"
     CDS             complement(4344..5387)
                     /locus_tag="PLI69_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOG41339.1"
                     /translation="MWKGRRLFFYSLAFIAGDLFGGWLTLPPALFLTTAALCAIMGLT
                     TKNRIITLITLFLLGAATLQISRIPTLSGESAIGHAAGSIKAEFSASLGKILSEGEEL
                     AIVRALTIGDKSDINRELRENYRKSGAMHLLALSGLHVGIIYKLVELMLFFIGGSISA
                     KRFKSILIILLLWGFAVITGLSSSIFRAVLMITIHELSGWRYSDKDGLTSLAASALII
                     TIVNPEAPREVGFQLSFLAVFSIFTIYPAIKGLMHTRILILEKLWNSICISLSCQMTT
                     GVVAFIYFGSFPAYFLITNILAIPLTGVSLYLTAAALISKSIPYAGHCAASALQFTVG
                     LLNKIIAIIAGLG"
     gene            5497..6708
                     /locus_tag="PLI69_02215"
     CDS             5497..6708
                     /locus_tag="PLI69_02215"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOG41340.1"
                     /translation="MIILVLNCGSSSLKYQVLDMKSDNDYSLLAKGLVERIGEPMGIY
                     NHQLPGKDKFSVEKPVPNHTVGISKVLELLTDKSLGVISSLKEIEAVGHRVAHGGEYF
                     SGSAAIDDEAITKIEKCAELAPLHNPANLLGIRAVQSLMPEIPQVAVFDTSFHQTIPD
                     YAYMYAIPYEYYQKYKIRRYGFHGTSHKYVAKKACNLVGMDISNSKIITCHLGNGSSI
                     TAIKNGRSIDTSMGFTPMEGLVMGTRSGSVDAGVVTFLEEKEKLSSSEINAILNKKSG
                     LLGISQVSSDMRDIESGAGSGNANAALAQEMLFYGIRKFIGAYAAAMGGLDMIVFTGG
                     IGENSSTTREQACEELEFLGVDFDKDVNRASRGKDVILSTPSSKVKVVAVTTNEELVI
                     AQDTMNIVKNK"
     gene            6724..>7500
                     /locus_tag="PLI69_02220"
     CDS             6724..>7500
                     /locus_tag="PLI69_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOG41341.1"
                     /translation="MITSFEQIFTQLRSKSRKRLVAAWGVDDHTITAARLAVDKGIVK
                     AILVGDADMINEVCKAEGINPSMFTIVDNKDEVQSIAQAVDMINAGEADILMKGLCST
                     DKYMRAILNKERGLLPPKAVLSHVSVLENPNYHKLIVLGDMAVIPAPDFKQKVAITNY
                     LVQTAHALGIEKPKVAAIAATEQMLEGMPACVEGAMLAKMADRGQIKGCYLDGPLALD
                     VAISKEAVEIKKLESNVAGDADCLLFPNIESANVFYKVNSQ"
BASE COUNT         2097 a         1612 c         1757 g         2034 t
ORIGIN      
        1 gtgcttctca ggtagaaaag cgctccgaaa agagtgttga agagcgttta tctgatgcaa
       61 tggtttccgg tgacacactt tatctggaag aggatattcg gcaggcactg gataaataca
      121 gtcccataga gatagttgag ggagctctcc tggagggaat ggagcgtgta ggactgcttt
      181 tcagtgaggg caagatgttt cttccgcagg taattaagtc tgctaaggtt atgcgcaatg
      241 cggtagctat tctccagcca ttgatggaga gtgatcaggt cgatattccc cggctacgcc
      301 ggaaaatgat actcgcaacg gcaaaaggcg atgttcacga cattgggaag aatattctgg
      361 gtattgtttt gagttgcaat aatatcgaga tcattgacct cggtgtaatg gtagataatg
      421 agactataat agaggccatt cactctcata aacccgattt tgtcggagtc agcggtctga
      481 taacaccctc attgcaatat atggagcaat tgtgccgtat gatggaaaaa gaggctctgg
      541 aactcccttt gtttattggt ggagccacca cttctgctct tcatactgcc gttaaacttg
      601 ccccgctgcg tacatcagct gtaatacaca cctcaggtgc ttccgattgt gccaatatga
      661 taaaccgtct ttcacgcgaa ccgcagaaaa ccctagaaga ggtaaaggct tctcaggagc
      721 agcttcgcaa cttgtatcat caaagcaatg aaacttttgt ctcgcttgaa gaggcacgtg
      781 ccagggtaga aaaatatcct cttgagagct atcaattgcc cgaagctttt ggggaagaca
      841 atctgcatgt gcgatttctt ccggtggaga ctcttgtcga taagatcgac tggaatatgc
      901 tgctcgcctt ttggggattc aaaggaggat atcctgctat cgtatattcc aatccggagg
      961 cagcgcaatg ctatgaatct gcaatagccg ccatagattc aatgattgtc gacagaagtg
     1021 tagacatatc aatggaagtg cgtttttttg atgcgcaaag tagtggtgag acaatcattt
     1081 tggatggtaa acatagattt gagatgcccc gatccacttc ggagaggagc gactataggt
     1141 cgcttgcaga ctttgttgtt cctgaaggat caggtttagt ctcaacggta ggtctttttg
     1201 ccataaaggt ggaggatctg caaagatgtg acgattgcag ggattacgaa catctgctta
     1261 gggaaagtat ctgtgcccgc ctcgcagagg cagctgccga atggatggaa gaacaggtta
     1321 gtgagggaac ggtgctgata agacctgctt tcggctatgc tgcctgtccc gaccattcac
     1381 agaaaaatct tgcctgccgg cttttggatg cctctgaacg tataggagtg caattgagcg
     1441 agacctctgc tctaattccc tccacttcgc tatgcggaat gctcatatcg catccgaaag
     1501 cccattatcc tattattaga aacaagaaag agtaactgaa atgaaggtaa tagatataat
     1561 taacagcaaa agcgggccat tcccgtcatt ggaatttgtt ccgccgttaa aaggtagtga
     1621 cattaaccgc ctctactctt ccattgaacc gtttatggag ttcaatccac cctttatcaa
     1681 tataacctgt catagagagg agatagaata tagggagaat gccgatggca caatttccag
     1741 gtatgtcttg acaaaacgtc ccggcacagt ggctattgcg gcagcggtaa tgaagagatt
     1801 cccaattgag gtagttcctc acataatttg tagtggaaca aacaggtttg ccatagaaaa
     1861 cgagttgatt gatctcaatt tcctggatat acagaatgtc atggcccttc gtggtgatgc
     1921 acccgcaggt cacaagaaat ttaaagccga gcctgacggt tgtagatata gcaacgaact
     1981 tgttgcgcag ataaaggcta tgaatgaggg taggtatatt gatgactcca ttaaggatgc
     2041 agtgccgaca aacttctgta tcggcgttgc cggttatccg gaaaagcact ttgaggctcc
     2101 taatctcgaa gtagatatcg aaaatcttaa aagaaaagtg gatgcgggtg ccgattacgt
     2161 catcactcag atgttttttg ataacaacaa gtattttgaa tttgttgcca aatgtcgcga
     2221 aaagggaatc aaagtaccca ttattcccgg actgaaaccc ctttccacag ctgctcatct
     2281 gacaaagcta cccgaagcct tttcaattga cataccgcag gaactggtta gtcttgtaaa
     2341 agagtgcaaa agtgaagacc aaatctatca gttaggtata gagtggtgtg tgagtcagag
     2401 caaagatctg attgcaaaag gagcacctgc aatccactac tatactatgg gacgcggcaa
     2461 caatgtcaca gaggttctca aaagggtctt ctgagagatt aaatgccgat gcccggaatc
     2521 ctttagattg ccttttaccg gcgctgatac agatatctta ctcgttatcc tttttctctg
     2581 aacgcttttt aggagggttc acggccactt ccacatggat aggtcggttc atgtgtttgc
     2641 tgccgttgag tgcggctatg gcggcagcac cctcttcatc attcatttcc acaaatccgt
     2701 agcctctgga acgtttggtc tcggggtcaa ctatgatacg tgcagatgta acagtaccat
     2761 actgttcaaa caactgtatc agatgttctc gcctcgcctt atagctgagg ccatagataa
     2821 aaatcatcat cgttaattat atcttggtaa ggttgtttca ttgttccgtc cggtagttaa
     2881 atcactgacc gtagcttgtt ccgtcaaagc aatgcgtaca gagagattct ttggggagtc
     2941 ctatggcctg cactatctct tttacactat taaaacatag ggtactcata tttagctttt
     3001 tccgtatata ttccaccata cgggcatgct caggacaact ctcacaggag tatttgtgta
     3061 aatcggccgg cccggcttta tctacctcat ctataaattt gcgggtaatc aactccagtg
     3121 aggaccttga tgatgagaag tttaggtatg gacagggata gatgataggc gggcaactta
     3181 tcctgatatg aacctcttga gctccgtgat tgtagagagc tttgacattg ttcttaagct
     3241 gcgtgccacg tacaattgaa tcgtcacaaa aggcaactct cttgttttta attatggagt
     3301 agttgggtat gagtttcatc tttgctacca gttctcttgt gctctgactg accggagtaa
     3361 agctgcgggg ccaggtagta ttgtatttga ccattgcggc acggaagggg atacctttgc
     3421 cgcgtgcata tcctaccgcc atacaggtac ccgagtccgg aatggatgag acaacatcga
     3481 gtggggtgtc atcttttgcc cccattatac tgccgagtct gttgcgtacg tcatctacat
     3541 tgatgccctc atagttactg gtagggtagc cataatagat ccataaaaat gaacagatct
     3601 gaagctcctt ctgaggagca agcaactgtg ttatgccatc agcagttagc agtaccgtct
     3661 cacccggtcc gagtatgtat tcactttcaa acccgatgtt ggggaatgag caatcttcgg
     3721 aggcgacggc catagagtct tcgtttcgcc ctatgataag tggtgtacgt ccatatttgt
     3781 ccctggctgc aataataccc tcagatgtga ggataagcat tgtcagcgaa cctcttattc
     3841 ggttttgtgc atattctatg ccttcggcaa atgaattctt ttcggtgaca aggtgagaaa
     3901 cgagctcggt ctggttggtc tttccggaac ttatttcagc aaaattctta ccttttgcaa
     3961 gaaattcttc ctcgagttct tctacattgt tgatcttacc aaccgtgacg attgcaaatc
     4021 tgccgaggcg agagttgatc agtataggct gtgcatcggt atcgcttatg actccgatac
     4081 cggaattacc gtcaaatttg tgaaggtccg attcaaattt tatcctgaaa taggaattct
     4141 ggatatcgtg aatggagcgt gcgaagagtc cgtcgcggtt gagaaccgcc attccggcac
     4201 gtttggtgcc tagatgtgag tgataatctg ttccgtaaaa aagttcggtt ttgcattcgc
     4261 gtttggatat tactccaaat agacctccca taatttcaga tttgtaattt aagccacaaa
     4321 gttagcataa ataaaaatat aaactatccc aatccggcaa taatggcaat aattttattc
     4381 aataatccca ctgtaaattg taatgccgaa gctgcgcaat ggccggcgta agggatactc
     4441 ttgctaatga gtgcagctgc tgtaagatag agcgataccc ctgtaagagg gattgcaaga
     4501 atgttggtga ttaaaaaata tgcgggaaaa ctcccgaaat aaataaatgc gacaacaccg
     4561 gttgtcatct gacaacttag acttatgcag atgctattcc agagtttctc caaaattagt
     4621 atgcgggtgt gcattaatcc ttttatcgca gggtagatgg taaaaatcga aaacactgca
     4681 aggaacgaga gctgaaatcc cacttcccgt ggtgcttcag ggttaacaat agtgattata
     4741 agtgcgcttg cagcaagtga agtgaggcca tccttatcgg agtagcgcca accggacaat
     4801 tcgtggatag taatcatcaa taccgctctg aagatagagg agctcagtcc ggtaattacg
     4861 gcaaatcccc atagcagcag tattattaaa atactcttaa atcgcttggc cgagattgaa
     4921 ccgcctataa aaaagagcat cagctccacc agtttataaa tgatacctac atgaagacct
     4981 gagagtgcaa gaaggtgcat ggcaccgctt tttcgataat tctctctcaa ttcccggttg
     5041 atatcgctct tgtcgcctat cgtcaatgcg cgtactatcg ccaattcctc tccctcgcta
     5101 agtattttac ccagagatgc tgaaaactcc gctttaattg agcctgccgc atgaccgatt
     5161 gccgattctc cgctcagggt aggtattctt gagatctgaa gagtagcagc gcccagcaaa
     5221 aagagagtaa ttagagttat aatcctgttt ttagttgtca gccccattat ggcacataag
     5281 gctgccgttg taaggaatag ggcaggtggc agtgtcagcc aacctccaaa taaatcgccc
     5341 gcgataaagg ctagcgaata gaaaaaaagt ctcctacctt tccacataat ccttccctct
     5401 atgtttcgga ttgcaatgta caattatttt ttgaaaggtc acctataatg actacttttg
     5461 ccggataaat ccgacaatta taaccagata tatattatga tcatacttgt actaaattgc
     5521 ggtagttcct ctttgaaata tcaagtgctt gatatgaaga gcgacaatga ctattcactc
     5581 ttggctaaag gccttgtaga acgtatcgga gaaccgatgg gtatttataa tcatcagtta
     5641 ccgggcaaag ataaattttc agtagaaaaa cctgtgccca atcacactgt tggtatctcc
     5701 aaagtgcttg aactgcttac tgacaaatct ctcggagtta tctcttctct taaggaaata
     5761 gaggccgtag gtcacagggt ggcacatggt ggagaatatt tttcaggcag tgcagcaatt
     5821 gatgatgaag ctatcacaaa gattgagaag tgtgcagaac ttgctccact ccacaatccg
     5881 gccaatttgc tcggtatccg tgccgtgcag tcccttatgc cggagattcc ccaggtagct
     5941 gtgtttgata cctcatttca ccagactatt cccgactacg catacatgta cgcaatccct
     6001 tatgagtact atcagaaata taagatccgt cgttacggat ttcacggcac aagccataag
     6061 tatgtggcaa aaaaggcctg caaccttgta ggcatggata tatcaaattc aaaaatcatc
     6121 acttgccacc tcggtaacgg cagttccata acagccataa agaatggacg ttccatagat
     6181 acctcaatgg gatttactcc tatggaggga cttgttatgg gtacaagaag tggaagcgtt
     6241 gatgcgggcg tagtaacatt tctcgaggag aaagaaaagc ttagctcatc tgagataaat
     6301 gcaattctga acaaaaaaag cggacttcta ggcatatcgc aggtctcttc ggacatgagg
     6361 gatattgagt cgggcgcagg aagtggcaat gccaatgcag cacttgctca ggagatgcta
     6421 ttttatggta ttcgcaagtt tatcggagcc tatgcagcag ccatgggtgg acttgatatg
     6481 attgtattta ccggcggcat tggagaaaac tcctctacta cacgtgagca ggcttgtgag
     6541 gagcttgaat tcctcggagt agattttgac aaggatgtca acagggcttc aagagggaag
     6601 gacgtaatcc tttctacccc ttcatccaaa gtgaaagttg ttgcggtcac caccaacgag
     6661 gagttggtaa tcgctcagga cacaatgaat atcgtaaaaa acaaataaaa tattttaaga
     6721 acaatgatta caagtttcga acagattttt acgcagttgc gttcaaaaag cagaaaacgc
     6781 cttgtcgctg cctggggtgt tgatgatcac acaattactg ctgcccgtct ggcagttgac
     6841 aaaggtatcg ttaaagctat tcttgtgggc gatgccgaca tgatcaacga ggtatgcaag
     6901 gcagagggta tcaacccctc gatgttcacc atagttgaca acaaagatga ggtccaatca
     6961 attgcacagg ctgtggacat gatcaatgca ggcgaagccg atatcctgat gaagggtctt
     7021 tgcagcaccg ataaatatat gcgtgccatt ctcaataaag agcgcggcct ccttcctcct
     7081 aaggcagttt taagccacgt gtcggtactt gagaatccca attaccacaa acttatcgta
     7141 cttggtgaca tggcagtaat ccctgcaccc gacttcaagc agaaagttgc catcaccaac
     7201 tacctggttc agactgcaca tgctctcggt atagaaaaac ctaaggtagc agctattgca
     7261 gccaccgagc agatgctcga gggtatgcct gcttgtgtag agggagcaat gcttgcaaag
     7321 atggctgaca gaggccagat caaaggctgt tacctcgatg gtccccttgc gctcgacgtg
     7381 gctatctcca aagaggctgt tgaaattaag aaacttgaga gtaatgtagc gggtgacgca
     7441 gactgcctgt tgttccccaa catagagtcc gcaaacgtct tctacaaggt aaattcacag
//
LOCUS       DAOSIE010000041         7472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9073_length_7472_cov_3.417150, whole genome shotgun sequence.
ACCESSION   DAOSIE010000041 DAOSIE010000000
VERSION     DAOSIE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9073_length_7472_cov_3.417150"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PLI69_02225"
     CDS             <1..680
                     /locus_tag="PLI69_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOG41342.1"
                     /translation="GNDRTIILNNNEGIYWLTEAIDKLCETSDQLLYGWNLMEGKSDI
                     SANRAFDGSELSLFKIQNLIIRGADEGEYTKIYVSPRHADVLVFNYCTNITLENLQLG
                     HYPEKGTCTGDVLSFSDCDGITISNCDLFGCGAIGIEAVLSKNFKINNSIIRDCSEND
                     LWLKECENFHFYNSKIKSKKYIFCSNSRGISFEKCTLPTSFDESITFINCEFFEWPGF
                     AYPDSGY"
     gene            700..1554
                     /locus_tag="PLI69_02230"
     CDS             700..1554
                     /locus_tag="PLI69_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOG41343.1"
                     /translation="MKKITILFVALVSFIAASCKSNSDTDLNQVITVNNAEEFIKALG
                     NDRTIILNNNEGIYWLTEAIDAAVEANLLKEYSEIKCLDENNRYTIPTDFSGILAYGE
                     ELWFQNMNNLTISGSDEENHTKIYIAPNYADVLLFIECTGITLENLELGHYPEEGECD
                     AAVLGLIDCDKVNINNCELFGCGTTGIRAIRSKNVKVNNSTVRDCSESNVDIRLCENI
                     RFYNCKLKSENDILCSDSKNISFEKCLVPVLTSADASFIECESFDWMEGDYVWIDHDF
                     EEEEEEGE"
     gene            1563..2591
                     /gene="hydE"
                     /locus_tag="PLI69_02235"
     CDS             1563..2591
                     /gene="hydE"
                     /locus_tag="PLI69_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOG41344.1"
                     /translation="MLSRKEITDLLLSKEPEIISDLHRRAADIKLRTIGPKVHLRGLI
                     EISNICRKDCLYCGIRKGNTLCERYTLTDEEVLTCAKHAYAAEFGSVVIQGGERTDSM
                     FTKRITSLLHRIKRIGDGALGITLSLGEQSAEVYKEWFEAGAHRYLLRIETSDPDLFR
                     KIHPDDGYHSYERRIEALMDLKSIGYQAGSGVMIGLPWQNTQHLASDLLFLKEMDFPM
                     IGMGPYVPHDDTPLTIMTNSDPGASFPSVAERLELSLKMISLLRILVPDINIAASTAL
                     EALHPKGRIMAIHAGANIVMPNITPSDYRKYYNLYDRKAILLEDLDLEDAEIGFGEWG
                     DSLHFNKA"
     gene            complement(2578..4626)
                     /locus_tag="PLI69_02240"
     CDS             complement(2578..4626)
                     /locus_tag="PLI69_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG41345.1"
                     /translation="MDKQNLEILNRYAINLNEKARQGKLDPVIGRDDEVRRVLQILSR
                     RTKNNPILVGEPGVGKTAISEGIAQRIVNGDVPENLKNKDLYSLDMGALIAGAKYQGE
                     FEERLKGVVKAVTESEGEIILFIDEIHTLVGAGRTSGAMDAANILKPALARGELRAIG
                     STTLDEYRKYFEQDKALERRFQMVMVDEPTPAESIAILRGLKERYENHHKVRIEDDAI
                     IAAVELSRRYITNRFLPDKAIDLIDEAASKLRLEINSVPEPIDELNRKIMQLEIEREA
                     VKREGKSPKLTAIQEDITRLSGERDVLMKQWEEEKKLLTAIQSNRQAIEEYRIQADDA
                     ERRGDYGKVAELRYGKIAEAKAQIDKLMAEKEHNDHPILNEQVTSENVAEVVAKWTGI
                     PVQKMLQSERDKLLNMEEVLHQRVIGQDEAIAAVSNAVRRSRAGLQNEKRPIGSFMFL
                     GSTGVGKTELAKALAEYLFDDENMITRIDMSEYQERHTVSRLIGAPPGYVGYEEGGQL
                     TEAVRRKPYSVILLDEIEKAHPDVFNLLLQLLDDGRLTDNKGHTADFKNTILIMTSNI
                     NPDLVKESFRPEFLNRIDEIIVFHELSPEHIRSIVKLQANLLQKRLEAMDIKISFTEE
                     FYDYMSVKGYDPAYGARPVKRVLQRELIDYLATLLLDGTLSKESEITATFVDGKLKLY
                     "
     gene            4810..6009
                     /locus_tag="PLI69_02245"
     CDS             4810..6009
                     /locus_tag="PLI69_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOG41346.1"
                     /translation="MKKILTLSLAALICFSAVAQSKDETKPEGFLFKTVKEHPITSVK
                     NQASTGTCWCFAALSFFESEAIRQGYKSDIDLSEMFVVSNAYVDKAIKYIRVDGHLNY
                     AQGSSFGDVLCVLKQHGIVPNSVMPGLNYGTERHMHGELAAGLKGYVDGILKNPNRKL
                     STAWIPGLKGILAAYLGPIPEKFQYEGKEYTPKSYMESLGINLDDYVDLVSWTHLPYY
                     EEHPVEVGDNWRWERAYNLPLDELMAVMYYAIENGYTFAWASDVSETGFTRDGIGIVP
                     DMDAIRKKTEEVGSDQAHWVGADPGTGRPIFKAPVPEIEVTAELRQQGYEEKTTTDDH
                     GMHIFGLAQDQNGTKYFMVKNSWGENSKYKGIWYVSETYVKYKTMDIMVHKDAIPREI
                     RKKLGIK"
     gene            6034..7161
                     /locus_tag="PLI69_02250"
     CDS             6034..7161
                     /locus_tag="PLI69_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOG41347.1"
                     /translation="MKRILLIVTAVIVAASAFSQTQTIYKFTPVKDIEVTPVKDQART
                     GTCWCFATISLLEAELIRQGKGYYDLSEMFVVRHNYNKRMSDNYLRLGRGNVGPGSLS
                     HMTINAIAEHGIVPESAYSGIAYDSDKHNHSELSAFVKALSEVSVQRKVRSPEYWKIH
                     EHLFDTYLGKLPEEFEYNGKKYTPKSFATMLGFDDLSDYVEITSLTHHPFYKQVPLEI
                     PDNWDHQLYYNVPLDEFMAIIDNALLNGYTVAWDGDLISKSFNHNRGIAIDPKPEFFE
                     EAVKFEKIYPELEATQELRQNYFETFQTVDDHLMHLTGLFKDQNGTKFYKTKNSWGTA
                     RNEEMGGYLYMSETYVKMRTLAILVHKDAIPKAIRKKMGIE"
     gene            7167..>7472
                     /locus_tag="PLI69_02255"
     CDS             7167..>7472
                     /locus_tag="PLI69_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG41348.1"
                     /translation="MKKHIPNIITLLNLTCGCCAVIMALWGLYLGSFLFMAAAVVFDF
                     LDGFSARLLKAYSDIGKELDSLADLISFGLAPSLILFNWYYNSGHRYTFLAFVPLAIT
                     "
BASE COUNT         2087 a         1725 c         1609 g         2051 t
ORIGIN      
        1 tcggcaatga ccgcactatc atcctcaaca acaatgaagg catttattgg ctgacggagg
       61 ctattgacaa attatgtgaa acaagcgacc agctgcttta cggctggaac ctaatggaag
      121 ggaaaagcga catttcggca aatagggcat ttgacggcag cgaactcagt ttgttcaaaa
      181 tccagaacct cataataaga ggggctgatg aaggggaata tacaaagata tatgtttcgc
      241 cccgtcatgc agatgtcctc gtttttaatt actgtaccaa tatcaccctc gaaaatcttc
      301 agctgggaca ctaccccgaa aaaggaacat gtaccggcga tgtactgagt ttcagcgatt
      361 gcgacggaat caccattagt aattgcgacc ttttcggatg tggtgccatc ggtattgaag
      421 cggtattgtc gaagaatttt aaaatcaaca actctattat ccgcgattgt tcagaaaacg
      481 atctgtggtt aaaagagtgc gaaaacttcc atttctacaa tagcaaaatc aaatcaaaaa
      541 aatatatttt ttgctccaat agtcggggta tttcattcga aaagtgcaca cttccgacct
      601 catttgatga gagtataaca tttatcaact gtgaattttt cgaatggccg ggatttgcct
      661 atcccgactc aggatactag tccataaaga tatattacta tgaaaaaaat tacaatttta
      721 tttgttgcac tcgtttcgtt tatagcagca tcctgcaaat caaatagtga tactgatctc
      781 aatcaagtaa tcacggtcaa caatgctgaa gaatttatca aagctctcgg caatgaccgc
      841 actatcatcc tcaacaacaa tgaaggcatt tattggctga cagaggccat tgatgccgct
      901 gttgaagcaa acttattgaa agaatattcc gaaattaaat gccttgacga aaataataga
      961 tatacaatac caacagactt tagcggtata ctggcatatg gtgaagaact atggtttcaa
     1021 aacatgaaca acctgacaat cagtggatca gatgaggaaa atcacacaaa aatatatatt
     1081 gcacccaact atgctgatgt tcttttattc atagaatgca caggtataac cctcgaaaat
     1141 cttgagttgg gacactaccc ggaagaggga gaatgtgacg ctgccgtact cggtttaatc
     1201 gactgtgaca aagtaaatat aaacaattgc gaactttttg gatgcggtac taccggcatc
     1261 agagcgataa gatcaaaaaa tgtcaaggta aataactcaa ctgtccgtga ctgctcggaa
     1321 agcaacgtag atatccgttt atgcgagaac atccgtttct ataactgcaa attaaaatct
     1381 gaaaacgaca ttctatgctc tgatagtaag aacatctcat ttgaaaaatg cctcgttcct
     1441 gttttgactt ccgcagatgc atcgtttata gagtgtgaaa gttttgattg gatggaagga
     1501 gactacgttt ggatagatca cgactttgaa gaagaagaag aagaagggga ataagcaaaa
     1561 taatgttaag ccgtaaggaa ataacagatc tgctcctaag caaggagccg gaaataatca
     1621 gcgaccttca tagaagggca gccgatatca aattgaggac tataggtcct aaagtccacc
     1681 ttaggggcct tatagagata tccaatatct gtcgtaaaga ctgtctctac tgcggtatcc
     1741 gcaaaggaaa taccttatgc gaaagataca cccttactga tgaagaggtt ctgacctgtg
     1801 caaagcatgc atacgctgcc gaatttggat cagtcgtgat tcaaggcggt gaacgcacgg
     1861 atagtatgtt cacaaagagg atcacatctt tgcttcatcg tatcaaacgc atcggagatg
     1921 gagctttggg catcaccctc tctctaggag aacagagtgc cgaagtttac aaagagtggt
     1981 tcgaggcggg ggctcacaga tatttgctcc gcatagagac ttcagaccca gatttattcc
     2041 gcaaaataca tccggatgat ggctatcatt catacgaaag gcgcatagaa gctctcatgg
     2101 atctcaaatc tataggctac caagccggaa gtggcgttat gataggactc ccctggcaaa
     2161 acacccaaca tctggcttcc gatctgctct ttttaaaaga aatggacttc ccgatgattg
     2221 gaatgggacc ctacgtaccc cacgatgaca ccccacttac tattatgaca aatagtgatc
     2281 ccggggcatc attcccttcc gtagccgaac gattggagct atccctaaag atgatatctc
     2341 tgcttagaat cctagtgcct gatataaaca ttgctgcctc cactgccttg gaagcccttc
     2401 atcccaaagg acgtatcatg gctatccatg ccggagccaa tatcgtgatg cctaacatca
     2461 caccttcgga ctaccgcaaa tattacaatc tctacgaccg caaggccata ctgctggagg
     2521 atttggacct tgaggacgca gagatcggat ttggggaatg gggagactcc ctacacttta
     2581 ataaagcttg agcttaccat caacaaaggt tgccgtaatc tcggactcct tgctgagggt
     2641 accgtccaat aggagtgttg ccaaatagtc tatcaattct ctctggagca cccttttgac
     2701 aggtctggca ccataggcgg gatcgtatcc ctttacactc atatagtcgt aaaactcttc
     2761 ggtaaaggaa attttaatat ccatagcctc cagtcgcttc tgcaacagat tagcttgcag
     2821 tttgacgatg ctgcgaatat gctctggtga aagctcgtgg aagactatga tctcatctat
     2881 cctattgagg aattccggtc tgaaagactc cttgaccagg tccggattga tattggaggt
     2941 cattatcaag atagtattct tgaagtcggc agtgtggcct ttattgtctg taagacgacc
     3001 gtcatccagc aactgcaaca acagattgaa aacatccgga tgtgccttct ctatctcatc
     3061 cagtaagatc acagagtaag gtttgcggcg taccgcttcg gtcagctgtc caccctcttc
     3121 atatcccaca taacccggag gagctcctat caaacgagag actgtatgac gctcctgata
     3181 ttcgctcatg tctatcctgg taatcatgtt ttcatcatcg aagagatatt cagcaagagc
     3241 tttagccaat tcggtctttc ccactccggt acttccgagg aacatgaatg agcctatcgg
     3301 tcttttttcg ttctgcaatc ccgctctact gcggcgcacg gcattagata ctgctgctat
     3361 agcttcatct tgtcctatta cacgctgatg gagtacctcc tccatattca agagtttatc
     3421 cctttcgctc tggagcattt tctgaacggg aattccggtc catttggcta ccacctcagc
     3481 tacattctcg ctggtcacct gctcgtttag gatagggtgg tcgttatgct ctttttcggc
     3541 cataagcttg tcaatttgtg ccttagcttc agctatctta ccgtatctga gttcggccac
     3601 cttgccgtag tctccacgac gttcggcatc atctgcctgt atgcgatact cttcgatggc
     3661 ctgacgattg ctctgaattg cagtcaaaag ctttttctcc tcctcccact gtttcatcaa
     3721 aacatctctt tcacctgata agcgggtaat atcctcctgt atagcggtaa gttttggact
     3781 tttgccctct ctctttaccg cctcccgctc aatttcgagt tgcattatct ttctgttcaa
     3841 ttcatctatt ggctccggta cggagttgat ttccaggcgg agttttgagg ccgcctcatc
     3901 tatcaggtct atcgccttgt ccggcaaaaa cctgtttgta atgtatctgc gcgagagttc
     3961 tacagccgca atgattgcat catcctctat gcgtaccttg tggtgatttt catatctctc
     4021 cttaagtccg cggagaatgg cgatggattc agctggagta ggctcatcca ccatcaccat
     4081 ttgaaacctg cgctcaagcg ccttatcctg ctcaaagtat ttgcggtact catccagagt
     4141 ggtggagcct atggccctga gctctcccct ggcaagtgcc ggtttgagga tatttgcggc
     4201 atccatggct ccggaagtgc gtccggctcc taccaaagtg tggatttcat caataaatag
     4261 gataatttca ccttcacttt cggtaaccgc tttaaccact cccttcaacc tctcttcaaa
     4321 ctcgccctgg tatttggctc ccgctatcaa tgcacccata tccagtgagt acagatcttt
     4381 attctttagg ttctcaggca cgtcgccatt gacaattcgc tgggcgatac cttcggaaat
     4441 ggctgtttta ccaacaccgg gctcgcctac tagtataggg ttgtttttag ttcttcggct
     4501 gaggatctgc agtactctgc gcacctcatc atcacgccct atgacagggt caagtttgcc
     4561 ctgcctagcc ttctcattga ggtttatggc gtatctgttc aaaatttcga gattctgctt
     4621 gtccatactt gttaattctg ttttttcgtt ttcatttatt tatatattat tagtttcaat
     4681 ttgtttgcca ccggttacaa actgactttt tgtcaagatt aaagcggcta ttagcggaga
     4741 aattattttg gataaaagag acatattcat taattttgtt ggatgcgaat aattttaatc
     4801 caatttataa tgaaaaaaat cctaactctc tcattagcag ctctaatctg cttttcagcc
     4861 gtagctcagt ccaaagatga gacaaagcct gaaggcttcc tctttaagac agttaaagag
     4921 catcccatta cctctgtaaa gaaccaggcc agtaccggta cctgctggtg ttttgccgca
     4981 ctctcatttt ttgagtccga agccatccgt cagggttaca aaagtgacat tgatctatca
     5041 gagatgttcg ttgtttcaaa tgcatacgtt gacaaagcta tcaagtacat ccgtgttgac
     5101 ggccatctca attacgcgca aggcagctcc ttcggtgatg tattatgtgt actgaagcag
     5161 cacggcatcg ttcccaattc cgtaatgccc ggtctcaatt atggaacgga gcggcacatg
     5221 catggagagc tcgctgccgg tcttaaaggc tatgttgacg gtatcctcaa gaatcccaac
     5281 aggaagctct caactgcctg gattcccgga ctgaaaggta tcctcgccgc ttatcttggc
     5341 cccatacctg agaaattcca atacgaagga aaagaataca ctccaaagtc ctacatggag
     5401 tcactgggca tcaatctgga tgattatgtg gatctggtat cctggacaca ccttccctat
     5461 tatgaagaac acccagtgga agttggagac aactggcgct gggagagagc atataacctc
     5521 cctctcgatg aactcatggc agtgatgtac tatgcaattg aaaatggcta tacttttgcc
     5581 tgggcatcgg atgtgagtga aaccggcttt accagagatg ggattggcat tgtccccgat
     5641 atggatgcaa ttcgtaagaa aactgaagaa gtgggctccg accaagcaca ctgggtaggc
     5701 gcagatcccg gcaccggcag acctatattc aaggcacccg ttccggaaat tgaagtgaca
     5761 gcggagctcc gccagcaggg ttatgaggag aaaacgacta ccgatgacca cggaatgcac
     5821 atttttggcc ttgcacagga ccagaatgga accaaatact tcatggttaa gaactcatgg
     5881 ggtgaaaata gcaagtacaa aggtatttgg tatgtttcag aaacttatgt gaagtacaag
     5941 accatggaca taatggtaca caaagatgct attccgaggg agatccgtaa gaagctgggt
     6001 ataaaataac aaatcagaaa taacaaccat actatgaaac gtattttact aatagtcaca
     6061 gctgtaattg ttgcagcatc ggctttctct cagacacaga ccatctacaa attcactcct
     6121 gtaaaggaca ttgaagtaac tcccgtaaag gatcaggccc gtaccggcac ttgctggtgc
     6181 ttcgccacga tatcacttct ggaagcagaa cttatacgcc aggggaaggg atattatgac
     6241 ctttccgaaa tgtttgtagt gcgtcacaat tacaacaaga gaatgagcga caactacctc
     6301 cgtcttggta ggggtaatgt aggtccgggc tcactatcac acatgaccat aaatgccatt
     6361 gcggaacacg gaattgtacc cgagagtgca tatagcggca tagcttatga ctcggacaaa
     6421 cacaaccata gtgaactgag tgcctttgta aaggctcttt ccgaggtaag cgttcagaga
     6481 aaggtacgct cacccgaata ttggaaaatt catgagcatc tcttcgatac ctacctcggc
     6541 aaacttccgg aagaatttga gtataacggc aaaaagtaca ctcccaagag ctttgccaca
     6601 atgctgggct ttgacgatct gagcgattat gttgaaatta ccagtttgac ccaccatcct
     6661 ttctacaaac aagttccgct ggagattccc gacaactggg atcaccagct ctactacaat
     6721 gtacctttgg atgagtttat ggccatcatc gacaatgctc ttctcaacgg ttatacagtt
     6781 gcatgggatg gagacctgat ttccaagtca tttaaccata accgcggaat tgccattgat
     6841 ccaaaacccg agttttttga agaggctgtg aagtttgaga aaatctatcc cgaactggag
     6901 gcgacacagg agctaagaca gaattatttt gaaacattcc agactgtgga tgaccacttg
     6961 atgcatctta caggtctttt caaagaccag aatggtacaa aattttataa aaccaaaaac
     7021 tcctggggta cagctcgtaa cgaggagatg ggcggttacc tctatatgtc cgaaacctat
     7081 gtgaagatga ggacattggc tatacttgta cacaaggatg cgatcccaaa ggcgatccgt
     7141 aaaaagatgg gcatagagta gcacttatga agaagcacat cccaaacatc ataacacttc
     7201 tcaacctgac ctgtggctgc tgcgcagtta taatggcctt atggggcctc tatctcggtt
     7261 cattcctttt tatggcagca gctgtagttt ttgatttttt ggatggcttc agtgcgagat
     7321 tgctcaaagc atattctgat ataggcaagg agctggactc tcttgcagat ctgataagtt
     7381 tcggtctggc tccttccctt atccttttca actggtacta caattcggga caccggtaca
     7441 cttttttggc atttgtccct ctggccataa ca
//
LOCUS       DAOSIE010000042         7442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9118_length_7442_cov_3.741167, whole genome shotgun sequence.
ACCESSION   DAOSIE010000042 DAOSIE010000000
VERSION     DAOSIE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9118_length_7442_cov_3.741167"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLI69_02260"
     CDS             complement(<1..706)
                     /locus_tag="PLI69_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOG41349.1"
                     /translation="MITLDELYKLYKKCGSLTTDSRKIPPGSMFFALKGDRFDGNDFA
                     LQALEQGAKCAIVDRATLDGASPGEGKGSCILVENVLKTLQALAHYNRTLFNIPVLAI
                     TGTNGKTTTKELTDAVLSQKYRTVATEGNLNNHIGVPLTLMNINEKTEIAIIEMGASV
                     PGEIAALVKIAEPTCGIVTNVGKAHLLGFGSFEGVKRTKGELYDYLRQNGGIIFFNEE
                     NNELKGMLANRPGVVTR"
     gene            complement(726..1160)
                     /gene="dut"
                     /locus_tag="PLI69_02265"
     CDS             complement(726..1160)
                     /gene="dut"
                     /locus_tag="PLI69_02265"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOG41350.1"
                     /translation="MKVRIVNKSAFDTPKYATPYSAGMDLRANIKEPVTLGSLCRTMI
                     PTGLFIELPEGFEAQIRPRSGLAAKHGISVTNSPGTIDADYRGEINVLLVNLSPDPFT
                     INPGERIAQMVISRHEKVEWVEVDDLENTERGAGGFGSTGIQ"
     gene            complement(1157..2641)
                     /locus_tag="PLI69_02270"
     CDS             complement(1157..2641)
                     /locus_tag="PLI69_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG41351.1"
                     /translation="MANPLKQLAGETAIYGLSTILARIVNFLLVPLYTRVLVQSDYGV
                     VTEFMAYIAILQVVLVLGLETGCFRFASREGVEKRSVFSTAFVIVTLFSLLFFVGVSL
                     FAGDIASGLGYEGFSNIIIYVAAILAIDSSTAIIFAKLRFEHKALRFAIIKTLKIFTE
                     VGVNLILYLVMPARFAANPDHWLLNFVSATPDFTYAIFSIFVSCIFAFVMLLPDLMHL
                     KFRIDRKLWRPLLLYSLPLMIAGLPGVMNDFLDRILMRFLNADPLTWRADLGVYQAGV
                     KIAVIMSLFIQMFRYAAEPFFFQKQRESGSKRVYADVMQWFVAFCMFGFLAIMLFMDE
                     IGLIIGKDFRVGLSIAPVMLMAYVLLGMLFNVDMWYKLAGKTRYAIYVALAGLAVTIV
                     VNVVFMPIYSYHAAAWGHLASYGVMLIISTLLGRKHYPIPYRWGRITLLVAIALAIYA
                     LSTLLPEMTLWPKLITHALLVIIYPALIAAMVIFERKKKQNPAI"
     gene            2714..3460
                     /locus_tag="PLI69_02275"
     CDS             2714..3460
                     /locus_tag="PLI69_02275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG41352.1"
                     /translation="MQKRLLLLFCATALIIAGCKGGNKSGNETSEGIESISSLSEADT
                     DETIENQDDMNREESKLQEGDDAATIKVNIPEEPIYAIKTTKGTIKVKLYKETPIHRD
                     NFVKLVNKHFYDDILFHRVINDFMIQVGDPNTKDSTKFDRIGMGGPGYTLPNEIAQCV
                     KAGKSHKKGALAAARRGDAVNPGKESSGSQFYIVQSEEGCKHLDGEYTIFGETIEGLD
                     IIDKIASVKTDRNGRPDNDISIISITLVNK"
     gene            3457..4044
                     /locus_tag="PLI69_02280"
     CDS             3457..4044
                     /locus_tag="PLI69_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG41353.1"
                     /translation="MMDFLELVKKSRSYRRFHQEKPVKREHMLKMVEAARFSPSARNV
                     QPLKYALYSTPEQCEIIFPTLAWAGYLTDWDGPREGERPSAYIIQLHDTTVSATYSCD
                     HGITAQSILLQAVELGYGGCIIGAVKREELSGLLHLPDHLKIINILALGVPKEKIVVD
                     DIKEGDYKYWREPDGTHHVPKRTLEELIFTPKNSD"
     gene            4050..4769
                     /locus_tag="PLI69_02285"
     CDS             4050..4769
                     /locus_tag="PLI69_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOG41354.1"
                     /translation="MRFTFRLLPLLLAAFIVFSCAPKQKGPDPRNPYGLEIINTYEGY
                     LESVAADPDMELVDLSTFIPGVVLDIRYATENNFTGKQIYTSPEAYARRPVAEALLAI
                     QEELATRGLGLKIFDAYRPYAATLYFYEVYQDTVFVASPTKGSNHNRGFAVDLTITNL
                     ETGEELEMPTEFDEFTEVAAAGYMDLPDLIIYNRTLLFDIMVRHGFTPYDGEWWHFNY
                     SKERDKYPIMDIPFEELDLKK"
     gene            complement(5006..5464)
                     /locus_tag="PLI69_02290"
     CDS             complement(5006..5464)
                     /locus_tag="PLI69_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOG41355.1"
                     /translation="MKVAKSYGDKGDVVINLSPSDPKDINFEGPVFIYFDGLPVPFFI
                     ESYQAKGGRWIVKFENIDTFEEAEEIVGKEIFVTLDEDQDDELSIIGMEVYDGDNALI
                     GVITDYTNAAGNEYIAVIHNGEEVLLPLHDDLIIEWGEEHITLKIPEGLL"
     gene            complement(5471..6079)
                     /locus_tag="PLI69_02295"
     CDS             complement(5471..6079)
                     /locus_tag="PLI69_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOG41356.1"
                     /translation="MAVKIRLARRGKKNYAFFHIVVADSRAPRDGRLIEQIGHYNPNT
                     NPATISIDREKALDWLNKGAQPTETARRILSYEGILLKKHLQGGVAKGAFSQEEADKR
                     WDAWKAAKEQKVKSKKQDIFNADLEAKKAAVSAEAKVNTERAEAIAKRKAEEAAAKAA
                     EEAAARAEEEALVAEAAAEPVVEAAPAAEAPAEETAEPVAEA"
     gene            complement(6276..6614)
                     /locus_tag="PLI69_02300"
     CDS             complement(6276..6614)
                     /locus_tag="PLI69_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOG41357.1"
                     /translation="MENPKIDTFLSYKGDYFPSARISTIREKLATASNSKNDIIQFYE
                     YKNPVVMLLISVVVGQLGIDRFMLGDIGLGVLKLITLGGCGIWWIVDLFTVQDRTREH
                     NFKKLIEILW"
     gene            complement(6895..7167)
                     /locus_tag="PLI69_02305"
     CDS             complement(6895..7167)
                     /locus_tag="PLI69_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG41358.1"
                     /translation="MAGLKEIETLLRDGKIEKAYDSLNDYLKKDPECAHAWYLLGGIY
                     RRQQLWGEAINAYNKAKLIEPDGPADAAIESIYDIIRFVNTDLMNP"
     gene            complement(7169..>7442)
                     /locus_tag="PLI69_02310"
     CDS             complement(7169..>7442)
                     /locus_tag="PLI69_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOG41359.1"
                     /translation="TTNCAVLGVALLVVNNEFTFGGDPHMLNLGQSVVYTVASAIGFG
                     LAITLFSGIREQLDLNNVPKAFRGVPIALIVAGIMAMAFMGFSGIV"
BASE COUNT         2104 a         1801 c         1608 g         1929 t
ORIGIN      
        1 tacgggtaac cacccccggg cgattggcaa gcatcccttt gagttcattg ttctcttcat
       61 tgaagaaaat gatgccacca ttctgcctca aatagtcgta gagctcccct ttcgtccttt
      121 ttactccctc aaatgagcca aatccgagca ggtgcgcttt gcccacatta gtcacaatac
      181 cgcaagttgg ctcagcaatc tttaccagtg ccgctatttc acccgggacg ctcgctccca
      241 tctctataat cgctatttcc gtcttttcat tgatgttcat caatgtcagt ggtacaccta
      301 tatggttgtt gagattgccc tcagtagcaa cggtcctgta tttctgcgaa agcactgcgt
      361 cagtaagttc cttggtagtg gtctttccat ttgttcctgt aattgccagc acgggaatat
      421 tgaagagggt acgattgtag tgtgcaagcg cctgaagggt cttcaagacg ttctccacta
      481 atatacaact ccctttgccc tctccgggag aggcgccatc gagtgttgca cgatctacta
      541 ttgcacattt tgccccctgt tccagcgcct gaagcgcgaa atcgttaccg tcgaaacgat
      601 cgcccttgag ggcgaaaaac atagatccgg ggggtatttt acggctgtct gttgtcagag
      661 agccgcattt cttatataat ttatagagtt cgtcaagcgt aatcataata attgccctcc
      721 tatatttact gtataccggt ggagccgaat cctcctgctc cgcgttctgt attttctaaa
      781 tcatccacct cgacccactc caccttttca tggcgggaga tcaccatctg tgcaattcgt
      841 tctcccggat tgatggtaaa ggggtcagga gagaggttta ccaggagtac attgatttca
      901 ccacggtagt cggcatctat ggtaccggga ctgtttgtga ctgaaatacc atgtttagcg
      961 gccaggccgc tgcgtggcct tatctgagcc tcaaaacctt ccggaagttc gatgaagagt
     1021 cccgtaggaa tcattgtgcg gcaaagggag cctagtgtta caggttcctt tatatttgcc
     1081 cgcaggtcca tccctgcgga atatggggtt gcatattttg gagtatcgaa tgcagattta
     1141 ttgactattc tgactttcat atagcgggat tttgtttctt ttttctttca aaaatgacca
     1201 tggcggcaat aagagcagga tagatgatca caagcaaagc gtgagtgatc agtttaggcc
     1261 agagagtcat ctccggtaat agcgtgctaa gggcataaat agccaatgca atagcaacca
     1321 atagggtaat gcgaccccac ctgtaaggga tagggtaatg tttacgaccg agcagcgtgc
     1381 tgataatcaa catcacccca taacttgcaa gatgtcccca tgcagcagca tggtaggaat
     1441 atatcggcat aaataccaca tttaccacaa tggtgaccgc aaggccggcc agagcgacat
     1501 agatggcata gcgggtcttt ccggccagtt tataccacat atcgacattg aagagcattc
     1561 ccagcaggac ataggccatc aacattaccg gagcaattga gagtcccact ctgaaatcct
     1621 taccgattat caggcctatt tcatccataa agagcattat cgcaagaaaa ccgaacatac
     1681 agaaggccac aaaccactgc atcacgtcag cgtagaccct tttacttcca ctctccctct
     1741 gtttctggaa gaaaaacggc tccgccgcat acctgaacat ctgaatgaag agcgacatta
     1801 tcactgctat cttgactccg gcctgataga cacccaggtc cgctctccag gtaagaggat
     1861 ccgcattgag aaaacgcatc aatatcctgt cgaggaaatc attcatcact ccgggaaggc
     1921 cggcaatcat aaggggaagg gaatatagca gcaacggtct ccacaactta cggtcgatac
     1981 ggaatttgag atgcatcaaa tccggcagga gcatcacaaa ggcaaatatg cagcttacaa
     2041 aaattgagaa gatagcgtat gtgaagtccg gagtagcgct cacaaaattg agcagccaat
     2101 gatcgggatt tgctgcaaat cgggccggca tcaccagata gaggatcaga ttgactccaa
     2161 cctcagtgaa gatctttagg gtcttgatga tggcaaatct caatgcctta tgctcaaatc
     2221 tcagttttgc aaagataatg gcagttgaag agtcaatcgc cagaatggcg gctacataga
     2281 ttattatatt ggaaaaccct tcatagccaa gtcccgaggc tatatcgccc gcaaagagag
     2341 agacacctac aaaaaagagg agtgaaaata gcgtaacaat tacaaatgcg gtagaaaaga
     2401 cggagcgctt ttcaacaccc tccctgctgg caaaacgaaa acagcccgtc tcgagaccaa
     2461 gtacaagcac tacttgaagt atggcaatgt aggccatgaa ctcggtcacc acgccataat
     2521 ccgattgaac caaaaccctg gtgtatagtg ggacaagcag gaaattgact attctggcca
     2581 atatcgtgct aagcccatag attgcggttt cgcccgcaag ctgtttcaat ggatttgcca
     2641 tcttggaaca ataattaatt agcaaactta cgaaataatc gctaattttg ggattcacaa
     2701 acccataaat gtcatgcaaa agagattatt attacttttc tgcgctacag cgcttattat
     2761 agccggctgt aaaggcggca acaaatccgg aaatgagact tctgagggga tcgagtccat
     2821 atcttccctc tccgaagctg atactgatga aacaattgag aatcaagacg acatgaatag
     2881 agaagaatca aaactgcagg aaggcgacga tgctgcaaca atcaaagtta atatccctga
     2941 ggagccaatc tacgccataa aaaccacaaa aggcacaatc aaagtaaagc tctacaaaga
     3001 gactcccatc caccgcgaca attttgtcaa actggtcaat aaacacttct atgacgatat
     3061 ccttttccac agggttataa atgattttat gatacaggta ggagacccca acaccaagga
     3121 ttcgactaag tttgacagaa taggtatggg cggccccggc tataccctcc ccaatgagat
     3181 cgcccagtgc gtaaaagccg gcaagagcca caaaaaggga gcattggcag cagcaagacg
     3241 cggtgatgcc gttaatcccg gcaaagaatc cagcggatcg caattttaca tagtacagag
     3301 cgaagagggc tgcaaacacc tggacggaga atacaccatc ttcggtgaga ccatcgaagg
     3361 actggatatc atcgataaaa tagcctctgt gaagaccgac cgcaacggcc gtccggacaa
     3421 cgatatctcc attatctcca tcacactcgt aaataaatga tggatttcct cgagcttgtc
     3481 aaaaagagcc gtagttaccg ccgtttccac caggaaaagc cggtaaaaag ggaacatatg
     3541 ctgaaaatgg tggaggcagc acgcttttca ccctctgcac gcaatgttca gcccctaaaa
     3601 tatgcacttt actccacacc ggagcaatgt gagatcattt tccccactct cgcatgggcg
     3661 ggctatctga ccgactggga tggtccgcgc gaaggagaac gcccctctgc ctatatcatt
     3721 cagctccacg atacgaccgt ctccgccaca tattcttgtg accacgggat aactgctcag
     3781 agtattctct tgcaggcagt ggagcttgga tacggtggct gcatcatcgg cgcggtaaaa
     3841 cgcgaggaac tttcgggtct gctgcatctg cccgaccatc ttaagatcat caatatcctt
     3901 gctctcggag tacctaagga aaaaatagtc gtagacgata ttaaagaggg ggattacaaa
     3961 tattggagag agccggacgg tacccaccac gtacccaaaa ggactctcga agaacttata
     4021 tttacaccaa aaaatagcga ttaaacccta tgagattcac ttttagactt ctgccgctgc
     4081 ttttagctgc ctttattgta ttttcctgtg ctcccaagca gaaaggtccc gacccgcgca
     4141 acccctacgg acttgaaatc atcaacacat acgagggata tcttgaaagt gtggctgccg
     4201 atcccgatat ggaattggtg gatcttagca ccttcattcc cggagtagta ctggatatcc
     4261 gttatgccac ggaaaacaat tttacaggca aacagattta taccagtccg gaagcatacg
     4321 cacgccgtcc tgtggctgaa gcgctgcttg ccattcagga agaacttgcc acacgcggac
     4381 tcggtttgaa aatttttgat gcgtatcgtc cttacgccgc aactctctat ttttacgaag
     4441 tctatcaaga taccgtattt gtggcctcac ccactaaagg atcaaatcac aaccggggat
     4501 ttgccgtaga tctgactatc accaacctcg aaaccggtga agaactggag atgcctaccg
     4561 aatttgatga gtttacggaa gttgctgccg ccggttatat ggaccttccc gatcttatca
     4621 tctacaaccg caccttactc tttgatatta tggtaagaca cggttttacg ccctacgatg
     4681 gcgaatggtg gcattttaac tatagtaaag agagggataa atatccgata atggatatac
     4741 cattcgaaga gcttgacctg aagaaataaa aatcaaagcg aattattttc gaatttttga
     4801 gaattattag cgattttttt tcgcgtggca cgcggtttgc tatttattaa atcgaatagt
     4861 tttttcatag gttaagtttt aggttaatag agatcagcgg tggccattcg gtcgccgctc
     4921 ctctttttaa atagtaccca attcttgctt attagaactt gttagaactt attcgagccc
     4981 ggtcgagctg cccggtcgaa cttatttata ataggccctc gggaatctta agagtgatgt
     5041 gttcttctcc ccactcaatt atcagatcgt cgtgcagagg caggagaact tcctctccat
     5101 tatgaatgac tgcaatatat tcgttgccgg cagcgttggt ataatcggta attactccta
     5161 ttaaagcatt gtcaccatca tacacctcca taccgataat cgatagctca tcatcctgat
     5221 cttcatcaag agtgacgaaa atctctttac caactatctc ttccgcctct tcaaaagtgt
     5281 cgatattttc aaacttcaca atccaacgtc cccctttcgc ctgataggac tctataaaaa
     5341 agggaaccgg cagtccatca aaatagatga acaccggtcc ttcaaaattg atatcttttg
     5401 ggtcggatgg ggacaagttg ataacaacat cccctttgtc accataagat ttcgcaacct
     5461 tcatctgaaa ctatgcttca gcaacaggtt ctgcggtttc ctcagcaggt gcttcggcag
     5521 cgggagcggc ctctacaacg ggttcagcgg cagcttcagc aacgagtgcc tcttcttcgg
     5581 cacgtgcggc agcttcctca gcagccttgg cagcagcctc ttcggctttc ctcttggcga
     5641 tagcttcggc tctctcggta tttacctttg cctcagcgct tacagcagcc tttttggcct
     5701 caagatctgc attaaagata tcctgctttt tggatttgac tttctgctcc ttagcggcct
     5761 tccatgcatc ccatctcttg tcagcctcct cctgagagaa agcacctttg gcaacacccc
     5821 cctggagatg tttcttaaga agaatacctt cgtaagagag gatacggcgg gcagtctcag
     5881 tgggctgagc acccttattg agccaatcca gagccttttc tctgtcaatg gagatagtag
     5941 cgggatttgt gttgggattg tagtgaccga tctgctcgat caggcgaccg tcacgcggag
     6001 cgcgggagtc agcaaccacg atatgaaaga aagcataatt cttctttccg cggcgtgcaa
     6061 ggcgaatttt tacagccatt tcaataaaaa attaaagtta ataattgtgt taacttcctt
     6121 atcttctcga gaaaaggtct gcaaagatag ggataattag gagaaaacaa aaaaaatcct
     6181 acggaaattt tctccgtagg actttcaatc tgaaaaatca aaatcggccg gcgcctctca
     6241 caaaaaaata ttgacagccg accggatctg taaaactacc acaaaatttc gatcaatttc
     6301 ttgaaattat gctctcttgt tctgtcttga acggtaaaga ggtcaactat ccaccaaata
     6361 ccgcagcctc ccaaagtgat aagttttaaa actcccaatc cgatatcacc aagcatgaac
     6421 ctgtcgattc caagctgacc tacaacaacg gaaatcagta gcatgaccac cggatttttg
     6481 tactcataaa actgaatgat atcatttttc gagttcgaag cagtagccag tttctcgcga
     6541 atagtcgaga tccttgcaga agggaaatag tcacctttat aagaaagaaa tgtgtcaatt
     6601 tttggatttt ccataattct attatttaat tgttaagtgt tttcggcata ctcgtgttat
     6661 tcagcatatt agccttccgg ccttaagcaa acctattttt atcccaaatg ctaaatgcaa
     6721 atataattaa aatattatgc aatttaaaga taagttaagc acaaccctct gatttgcact
     6781 acaaaaagag cggcctgtaa aagttcaaac ttgctctttt caggccgtcc taataggtca
     6841 aatatggcgt ttataaatgt ttacgccaga aaaatatgcg ttaaaaatcc tctatcaggg
     6901 attcatcaaa tccgtattga caaatctgat gatgtcgtag atggattcga tggcggcgtc
     6961 ggcggggccg tccggttcaa tgagtttggc cttgttgtag gcatttatgg cctctcccca
     7021 aagttgctgg cgacgataga tgccgccgag cagataccag gcgtgagcac actccgggtc
     7081 tttcttcaaa taatcattca gagagtcgta agccttctct atcttgccgt ccctcagcaa
     7141 tgtttctatt tcctttaatc ctgccatatc atacaattcc tgaaaatccc ataaatgcca
     7201 ttgccataat gccggcaact atcaatgcaa tgggaacacc tctaaaggcc tttggaacat
     7261 tgttcaggtc gagttgctcc ctaatgccgg agaagagggt tatcgcaagg ccgaaaccga
     7321 ttgcgctggc tacggtatag accaccgact ggcccaaatt gagcatgtga gggtcgccac
     7381 cgaaggtaaa ttcattgttt acaacgagca aagccactcc cagtacggca cagttggtag
     7441 ta
//
LOCUS       DAOSIE010000043         7431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9135_length_7431_cov_3.855613, whole genome shotgun sequence.
ACCESSION   DAOSIE010000043 DAOSIE010000000
VERSION     DAOSIE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9135_length_7431_cov_3.855613"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PLI69_02315"
     CDS             <1..1299
                     /locus_tag="PLI69_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG41360.1"
                     /translation="DPLTSEFWGSHIKNKGINGYLKLGIPLNEENSQNIAIVADFNYH
                     NMDSNYGLKTYDGTQKSLFLNVIYQNQINEYHRLSFGLSGQDDDFREDMNDHRTYIFT
                     PVPSPEPIIYNIDLSRRERSVGLYGEYNYTLDDKVTFVGSLRGDYNDIHGFLFAPRFT
                     LKYSFTDDIIFRLLGGRGFRTANIFADNIGIFSTGRIIIFDDTLSEKRNLLEDAWTYG
                     GNITFYLPFGYENNTYLSFDYFRNDFLKQVIVDQEFDMVQALVYNLDGKSFTNTYQVD
                     FSVDPIERFNIVTTFRYTDAKVTLRGQGLVERPLTSRFKAVFNAQYATAMNKWTFDFT
                     AQLNGKTKLPNFMAGQDKVIEIDGEYFSPIYPMFFAQVTRKFRGIDIYVGGENLGNYR
                     QRNAIINADKPFSEAFNASAIWGPLSGMKIYAGLRLTIWK"
     gene            1352..1678
                     /locus_tag="PLI69_02320"
     CDS             1352..1678
                     /locus_tag="PLI69_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOG41361.1"
                     /translation="MKQFFKITLVLALVALFSFNAGAQNEKKKQANNAEVTFVSNIDC
                     AGCVKKIEGNIPYEKGVKDLKVNLDNQTIYIKYDSTKTDKEKLAAAIVKLGYTAEEKV
                     VEEPKK"
     gene            1688..2845
                     /locus_tag="PLI69_02325"
     CDS             1688..2845
                     /locus_tag="PLI69_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41362.1"
                     /translation="MKTSVIKIVVLMALSILLVSCNDVKKDDFKVWAWLAGSTTMSEE
                     QLDHYFSEAARAGIDAIILECHGARPEVIGDSASFRDEAAILILENAMVYAKKYDIEL
                     HAWMWTTNRAELSLRKAHPEWYQVNAQGESCADIKLYNREHYRFLCPTRPEVTGYLKE
                     RVREIAEVEGLAGIHMDFIRYPDAILPYRLQESRGVVQDKVYPLWDFCYCDECRAAFK
                     EQSGIDPLDIGDPTSDEAWMQFRYDRMAEMASAIAAEIKACGKVASSAVFASPYESRK
                     LVRQDWANFRNHDYIFPMIYHKFYFDEDAWVETATREGVEALRAVGNDAVLCSGLFVG
                     HVPADRIEEFIGYTENGGSAGICFFSIEEMLKREGYVDSLRNVVEKIRLQD"
     gene            2903..5407
                     /locus_tag="PLI69_02330"
     CDS             2903..5407
                     /locus_tag="PLI69_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOG41363.1"
                     /translation="MKKLFLSAIVLLLACSAVAQSWVRINQVGYLPDDIKVAVLISTD
                     EASSEFDVRDAITDKIVFKSSGIKANASKWALKSAFRLDFSPVTSEGSYYITTNGTKS
                     PVFRIGADVYDGIADYLLLYMRQQRCGDNPYNDTLCHQHDGYIIYHPTKSGQKIDVTG
                     GWHDATDYLQYMTTSSTTIYQMAFAYKYAKDKSVFKDQYDARGRKGSNGIPDILDEVK
                     WGLDWLDKMNPAPREMYYQIADDRDHAGFRLPHRDTVNYGYGPGKGRPVYFVTGQPQI
                     AHKKVNRTTGVSSAAGKFASTFSLGADVIKEFYPEFAAKIERKVQDAYDFALEFPGNT
                     QTACVVSPYFYEEDTWVDDIELAAATIYSINGEEYWKKQAAYWGELEPVSPWMERGRG
                     PGKEYHHYQWYPFINLGHYLLASSNDKKVSTEFSEYMRQGLQYLRDRAGDEPFMHGVP
                     YLWCSNNLTSAAVTQARLYEEATGDTQFREMETALRDWLLGCNPWGTTMICGYPEAPV
                     EGYDFPSDPHSSYLLVNGDNTYGGLIDGPIYNILFEERAGFSLTKPDQFAIFNKGIAV
                     YHDDIGDYASNEPTMDGTAGLSYYFASLEGRGNALKSAPEMLKDNYGAVVRVNPQSKD
                     IYLVFTADTQFNGAESILNTLKKNKIKAGFFLTGNCLRKPGHKAIIERIIAEGHYVGG
                     HSDNHVLMADWGADRATLMTTDSIITDIRRNFEAFAAFGIEPKDALWYIPAYEHYNRE
                     NVALLNAYGLKTFNYTPGTGTPADYTVPSMKNYKPSQQLIEGLYAFEKEKGLGGAIIL
                     IHPGISVERPDPLYERLDEIIRTMKKKGYRFESFKN"
     gene            5417..6271
                     /locus_tag="PLI69_02335"
     CDS             5417..6271
                     /locus_tag="PLI69_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOG41364.1"
                     /translation="MYKIVKKNSLTPVICLMDVEAHRMAASAQPGQFLIVRTHEQGER
                     IPLTICDYDKERGTVTIVTQAVGASSRMICHLEEGDSFTDFVGPLGKPSEFIHWPDEE
                     IAGKKFLFVAGGLGAAPVYPQAKYLHAKGAHVDVIMGARNSELLIFEKEMGEVSDNLY
                     ICTDDGSKGEKGLVTNIIDKLMAEGKSYDHAVAIGPMIMMKFATLTMKKYNIPLTVSL
                     NTIMVDGTGMCGACRVTVGGQTRFACVDGPEFDAYQVDFDEAMRRQSMYKELEVEADH
                     KCNIGLHR"
     gene            6283..>7431
                     /gene="gltA"
                     /locus_tag="PLI69_02340"
     CDS             6283..>7431
                     /gene="gltA"
                     /locus_tag="PLI69_02340"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOG41365.1"
                     /translation="MKSKMTRIPVREQDPKVRATNFDEVSYGYNEEEAQAEASRCINC
                     KNPRCVANCPVSIKIPQFIQQVTLGNYTEAARIIAEDNSLPAVCGRVCPQEVQCEGAC
                     ILGIKGEPIAIGKLERFVADWSRENGVRFTEKAPSNGIKVAIVGSGPAGLACASDLAK
                     LGYEVTIFEVLHKAGGVLQYGIPEFRLPKEEVVEVEIDNVRALGVKIETNVVVGRTIT
                     IDELFEKENFKAVFIGSGAGLPKFMGIPGENLNGVFSANEFLTRSNLMGAYSGESDTP
                     IYTGTRVAVVGGGNVAMDAARTALRLGAETTIVYRRTEKELPARVEEVHHAKEEGIDF
                     RMLTNPVEILGNEKGWVTGLRCIKMELGEPDDSGRRRPVPVAGSEFDIE"
BASE COUNT         2074 a         1552 c         1829 g         1976 t
ORIGIN      
        1 gaccccctga caagcgaatt ttggggctca cacatcaaga ataaaggcat caacggctat
       61 ttgaaattgg ggattcccct taatgaagag aatagtcaga atattgcaat tgttgccgat
      121 ttcaactacc acaatatgga ttcaaactat ggattgaaaa cctacgatgg tacccagaag
      181 tccctcttcc taaatgtcat ttatcaaaat cagataaatg aatatcaccg tctctcgttc
      241 ggtctttcgg gtcaggatga cgattttagg gaagatatga atgatcacag gacatatata
      301 tttactcctg ttccttcacc ggaacccatt atatataata ttgaccttag tcgcagggag
      361 aggtctgtag gcctttacgg tgaatacaac tatactcttg acgataaggt gacattcgtg
      421 ggaagtctca gaggtgatta caacgatatt catggattcc tttttgcccc aagattcaca
      481 cttaaatatt cctttaccga cgatataatc ttcagacttc tcggaggcag gggattcaga
      541 actgccaata tatttgcaga caatattgga atcttctcta caggcaggat aataatattt
      601 gacgatacgt tgagtgagaa acgcaatctg cttgaggatg cctggacata cggtggcaat
      661 atcacattct atctcccttt cggatacgaa aacaacactt atctaagttt tgactacttc
      721 agaaacgatt ttctcaaaca ggtaatagta gatcaggagt ttgatatggt acaggcgctt
      781 gtttacaatc ttgacgggaa atcttttacc aatacctatc aggtggactt ttctgtagat
      841 cctatcgaga gattcaacat tgttaccaca ttcagatata ctgacgcaaa ggtgactctg
      901 agagggcaag gcctcgttga aaggcctctt acaagccgtt ttaaggcggt tttcaatgct
      961 cagtatgcta cagcgatgaa caaatggact tttgacttca ccgcacagct taatggaaag
     1021 accaaattgc ccaactttat ggccggtcag gataaggtaa tagagataga tggagagtat
     1081 ttctcaccca tatatcccat gttcttcgct caggttaccc gcaaattcag aggcatagat
     1141 atatatgtgg gcggtgaaaa cctcggcaat tatcgtcagc gcaatgccat tatcaatgcg
     1201 gacaaaccct tttcggaggc tttcaatgcc agcgctattt ggggccccct ttcaggtatg
     1261 aagatttatg cagggcttcg cctcacaatc tggaaatagg gtttgatttt gaatagtata
     1321 aaaatcatca aacaataaaa caacaatcac tatgaaacaa ttttttaaaa taaccctcgt
     1381 acttgccctt gtggcgctat tttccttcaa tgccggtgct cagaatgaaa aaaagaaaca
     1441 agccaataat gctgaagtta cctttgttag caacattgat tgcgctggtt gcgtaaagaa
     1501 aattgagggc aatatcccct acgaaaaagg tgtaaaggat ctaaaggtca atctcgacaa
     1561 tcagaccatc tacatcaaat atgactctac caagaccgac aaggagaaac ttgccgcagc
     1621 aatagttaaa ttaggctata ctgccgaaga aaaggtggta gaagagccta aaaaataact
     1681 tttcgccatg aagacttctg tcatcaagat agttgtcctt atggcattat cgatactttt
     1741 ggtatcttgc aatgatgtga aaaaggatga cttcaaggta tgggcctggc ttgcaggcag
     1801 taccacaatg agtgaggagc agctcgatca ctatttcagc gaagccgccc gcgcagggat
     1861 agatgcgatt atcctcgagt gccacggcgc caggccggag gtgatcgggg atagcgcctc
     1921 attccgcgat gaggctgcca ttctcattct ggagaacgcc atggtatatg ctaagaaata
     1981 cgatatagag cttcatgcct ggatgtggac caccaataga gccgagttga gcttgagaaa
     2041 ggcacatccc gagtggtatc aggtcaatgc tcagggagaa tcctgtgctg acattaaact
     2101 ctacaacaga gaacactacc gctttctctg ccccactcgt cccgaagtta ccgggtatct
     2161 aaaggagagg gtgagggaga tagcagaggt ggaaggtctc gcaggtattc acatggattt
     2221 tatccgctat cctgatgcaa ttctccctta tagattgcaa gagtccagag gagttgtaca
     2281 ggacaaggtc tacccgttgt gggatttctg ctattgtgat gagtgcaggg ccgccttcaa
     2341 ggagcagagt ggtatcgacc cgttggatat cggggatcct acttccgatg aagcctggat
     2401 gcaattccgt tacgatcgta tggcagagat ggcatcggct attgctgccg agataaaggc
     2461 ttgtggaaag gtggcttcgt cggcagtatt tgcttctcct tacgaatcca ggaagttggt
     2521 gcgtcaggat tgggccaatt tccgtaacca tgattatatc ttccccatga tataccacaa
     2581 gttttatttt gatgaagatg catgggtaga aaccgctacc agagagggtg ttgaggcact
     2641 ccgtgctgta ggcaacgatg cagtgctctg ctcgggtctc tttgtgggtc atgtgccggc
     2701 tgataggata gaggagttta tcggctacac cgaaaatggc ggctccgccg gtatctgttt
     2761 tttctctatt gaggaaatgc tcaaacggga gggctatgtg gattctttga gaaatgtggt
     2821 tgagaagatc agattgcagg attaaagaaa aaagcagtac ctttacaagt tataaactat
     2881 acactgacag atactgcttt ttatgaaaaa actcttctta tcagcgattg ttttgcttct
     2941 ggcttgtagt gccgtagctc aatcctgggt acgtatcaac caggtcggct atctgcccga
     3001 tgatataaag gttgcggtgc tcatttccac ggatgaggct tcatccgagt ttgatgttcg
     3061 tgatgccatc acggataaaa ttgttttcaa aagtagcggc attaaggcca atgcctccaa
     3121 atgggcttta aaaagtgcct tcagactcga tttttctccg gttacctccg aaggctccta
     3181 ttatattacc acaaatggga ccaaatcgcc agtattccgc atcggcgccg atgtctatga
     3241 tggtattgca gattacttac tgctctatat gcgtcagcag cgttgcggag acaatcccta
     3301 caatgatacc ctctgccatc aacacgatgg ctatatcata tatcatccca caaagagtgg
     3361 acagaagata gatgtcacag gaggctggca tgatgctacc gactacctcc aatatatgac
     3421 tacctcttcc acaaccatct atcagatggc ttttgcatat aagtatgcaa aggataagag
     3481 tgttttcaaa gatcaatatg atgctcgcgg acgaaaaggt tccaacggta ttcctgatat
     3541 cctcgatgag gtaaaatggg gtttggactg gctggacaag atgaaccctg ccccgaggga
     3601 gatgtattac cagatagctg atgatagaga tcacgccggt tttcgtcttc ctcaccgtga
     3661 tacggttaac tacggctacg gccccggtaa gggcagacct gtctattttg tgacgggaca
     3721 acctcagatt gcccacaaaa aggtcaacag gaccacggga gtctcctctg ctgcgggtaa
     3781 gtttgcctct actttcagtc tcggagcaga tgtgataaag gagttttatc ccgaatttgc
     3841 ggcaaaaatc gagagaaagg tgcaggatgc atacgatttt gctttggaat ttcccggcaa
     3901 tacgcagacg gcatgtgtgg tttctcccta tttctatgaa gaggatacct gggtggatga
     3961 tattgagttg gcggcagcta caatttactc tataaacgga gaggaatatt ggaaaaaaca
     4021 ggccgcttat tggggcgaac tggagccggt ttctccctgg atggaacgcg gacgtggtcc
     4081 cggtaaagaa taccaccact atcagtggta tccttttatc aatctgggcc attatcttct
     4141 tgcttcaagc aatgataaaa aggttagcac cgagttttcc gaatatatga ggcagggact
     4201 gcaataccta cgcgacaggg cgggtgacga gccatttatg cacggagtgc cttatctctg
     4261 gtgctccaat aatcttactt ccgctgccgt tactcaggcg cgtctctacg aagaggctac
     4321 cggtgatact cagttcaggg agatggagac ggcgctcaga gattggctct taggttgcaa
     4381 cccgtggggt acaaccatga tttgcggtta tccagaggca ccggtagagg gttatgactt
     4441 cccctccgac ccccattctt cctacttgct ggttaatggt gataatactt acggaggtct
     4501 gatagatggc cccatttata acattctctt tgaggagaga gccggttttt cacttaccaa
     4561 acccgatcag ttcgctattt tcaacaaagg aattgcagtc tatcacgatg atatcggaga
     4621 ttatgcttcc aatgagccca cgatggatgg aaccgccggt ctctcatatt actttgcatc
     4681 gcttgaaggg cgcggtaacg ctctgaaaag tgcgccggaa atgcttaagg acaattatgg
     4741 ggcggtggta agagtcaacc ctcagagtaa ggatatctac cttgtattca ctgcggatac
     4801 gcagttcaat ggtgctgaat cgatactgaa taccctcaaa aagaataaaa taaaagcggg
     4861 cttcttcctt accggcaatt gccttcgtaa gcccggacat aaagccatca tagagcgaat
     4921 cattgccgag gggcactatg ttggcggtca ttccgacaac catgtgttga tggccgattg
     4981 gggtgccgat agagctactt tgatgaccac ggacagcatt attacggaca ttcgccgcaa
     5041 ttttgaagca tttgccgcct ttggcataga acctaaagat gcattgtggt atattcccgc
     5101 ttatgagcat tacaataggg agaatgtcgc tctgctaaat gcctacggcc ttaaaacatt
     5161 caattatacc cctggtaccg gcacacctgc cgattacact gttccaagca tgaagaatta
     5221 caaaccttct caacagttga tcgagggact ttatgctttt gaaaaggaga aaggtctcgg
     5281 aggagctatc atactcattc atccgggtat aagtgtagag cgtcctgatc ctctttatga
     5341 gcgtctggat gagattatca gaacaatgaa aaagaaagga tatagattcg aatcatttaa
     5401 aaactaatat aaatatatgt ataaaatcgt taaaaagaac tctttgactc ctgtaatatg
     5461 tctgatggat gtcgaggccc acagaatggc tgcttcagct cagccgggac aatttctaat
     5521 agtcaggacc catgagcagg gtgaaagaat tcctcttacc atttgtgatt atgataaaga
     5581 gagaggtacg gtaactatag ttactcaagc tgtaggtgct tcaagtcgta tgatttgcca
     5641 ccttgaagag ggtgattctt ttactgactt tgttggccct ctggggaaac cctctgagtt
     5701 tatccattgg ccggatgagg agattgcggg caagaagttc ctctttgttg cgggtggtct
     5761 gggtgcggct cccgtctatc cgcaggcaaa atatttacat gccaaaggag cccatgtgga
     5821 tgtaataatg ggtgccagaa atagcgaatt gctgattttt gaaaaagaga tgggagaggt
     5881 atccgacaat ctctacattt gtaccgatga tggttcaaaa ggtgaaaagg gacttgttac
     5941 aaacattatc gataagctca tggctgaggg caagagttat gaccatgctg tggccatagg
     6001 tcctatgatt atgatgaaat ttgctaccct gacaatgaag aagtacaata ttcctttgac
     6061 cgtaagttta aacactatta tggtggatgg tacgggaatg tgcggtgcct gccgtgtgac
     6121 tgttggaggt cagactcgtt tcgcctgcgt tgacggtccc gaatttgatg cctatcaagt
     6181 tgactttgat gaggctatgc gtcgtcagtc aatgtataag gagctggagg tggaggccga
     6241 tcataaatgt aatattgggt tgcacagata atagaagaga atatgaaatc gaaaatgaca
     6301 agaatacccg ttagggaaca agatcctaaa gtaagggcta ccaactttga tgaagtatct
     6361 tatggatata atgaggagga ggcgcaagct gaagcatccc gctgtataaa ctgtaaaaat
     6421 cccaggtgtg tagccaactg cccggtatct ataaagattc cgcagtttat ccagcaggtc
     6481 acactcggca actatactga agcagcccgc atcattgcgg aggataatag tctccccgca
     6541 gtttgcggac gtgtctgccc tcaggaggta cagtgtgaag gggcctgtat acttggaatt
     6601 aagggcgaac ccatagccat aggaaagtta gagcgctttg tggccgactg gagtcgtgaa
     6661 aatggtgtga ggtttactga aaaagccccc tcaaatggta ttaaggttgc gattgtaggt
     6721 agcggtccgg caggtcttgc ctgtgcaagc gaccttgcaa aactaggcta tgaagtgacc
     6781 atttttgagg ttcttcacaa ggcaggcgga gtcctccagt atgggattcc tgagtttagg
     6841 cttcctaaag aggaggttgt agaggtggaa attgataatg ttcgtgcact tggagtaaaa
     6901 atagaaacca atgtggttgt aggtcgtaca atcaccattg atgaactttt tgaaaaagaa
     6961 aacttcaaag ccgtctttat cggttccgga gcggggcttc ccaaatttat gggtattccg
     7021 ggtgaaaatc ttaacggagt cttctctgca aatgaattcc tgacgcgcag caatctcatg
     7081 ggtgcctatt ccggtgagtc cgatacaccc atctatacgg gaaccagagt ggctgttgtc
     7141 ggtggcggca atgtggctat ggatgctgca cgtacggcac tcagacttgg agcagaaact
     7201 actattgtct atcgtcgtac ggaaaaggaa cttcctgcac gtgtagagga ggtgcatcat
     7261 gcaaaagagg aggggataga tttcagaatg ctcaccaatc cggttgaaat cctgggaaat
     7321 gaaaagggtt gggttaccgg tctcagatgc ataaaaatgg agcttggaga gccagatgac
     7381 agcggtagaa gacgccccgt acctgttgca ggttcggaat ttgatataga a
//
LOCUS       DAOSIE010000044         7427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9142_length_7427_cov_4.065789, whole genome shotgun sequence.
ACCESSION   DAOSIE010000044 DAOSIE010000000
VERSION     DAOSIE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9142_length_7427_cov_4.065789"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLI69_02345"
     CDS             <1..333
                     /locus_tag="PLI69_02345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG41366.1"
                     /translation="TVKIRHNSVYTTAYLHLSKYASGLKIGSRGSQGQVIGYVGSTGM
                     STGPHLDFRVWKNGSPINPLKMESPPAEPIREEHKEKFEASRDSLRHLASNYLLDEYY
                     RKYVLNLL"
     gene            complement(342..809)
                     /gene="mscL"
                     /locus_tag="PLI69_02350"
     CDS             complement(342..809)
                     /gene="mscL"
                     /locus_tag="PLI69_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOG41367.1"
                     /translation="MSKFLKEFKEFAMKGNVMDMAIGVVIGAAFGAIVTSLVGDLIMP
                     LISWATGGIDFTEWKITLRAAVEATETTEAVEGLYVSYGKFINVLFNFIIIAFSIFLV
                     IKGINKMSKKFESEKTKKAKEEAEAKAKAAAETPAPESDEVKILKEIRDALKK"
     gene            complement(844..1473)
                     /locus_tag="PLI69_02355"
     CDS             complement(844..1473)
                     /locus_tag="PLI69_02355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOG41368.1"
                     /translation="MDHLGRYLIAHSSSQGEALEWLTRQTHLRTNRGRMLSGPILGKF
                     LEMVTKMISPSRVLEIGTFTGYSTIAISRGLAPGGTIDSIEINDELEDLIREGYEKAG
                     ITDRVRLIFGDVLQVLPTLEEGYDLIFLDANKREYPHYFEAAVGLLRSGGFILADNVL
                     WDGKVYEESPTTDAQTHGISEFNRIVSEDPRVENVIIPLRDGINIIRKL"
     gene            complement(1480..2742)
                     /locus_tag="PLI69_02360"
     CDS             complement(1480..2742)
                     /locus_tag="PLI69_02360"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG41369.1"
                     /translation="MDNYSQNIYIQTLPCGIQFAFKRTPSPVAYCALSIRSGTRHEPE
                     ELPGTAHFAEHMLFKGTSRHCQREINNRLERLGGELNAFTTKEETVVHSTTLKEDIGK
                     SVDLLFEIVLSSTFPEKELIKELSVIIDEINMYKDSPAERIYDEFEELLFGNHPLSRS
                     ILGNAKSLKKISTEDLRNYTRSRFIPTALCFTAVSDMDPEIVVNMVEKAISRHYTGPK
                     EIETPLPTPYESNSSLDKGSLFNKEVSRKNHQAHCILGTSAYSLYDKNRLALLLLCNM
                     LGGPSSNSRLNQSLRERSALVYTVESSYTQYSDTGTFAIYFGCDKGDVEKCLSLTDKE
                     IGYFREGRLTEAQLKAAKKQLLGQHAISSDNGEVQALSMGKSLLSYGNVMPDSEIHRL
                     IEEITAEDIRRVACEVLAPERLSTLIYR"
     gene            2824..4539
                     /gene="dnaX"
                     /locus_tag="PLI69_02365"
     CDS             2824..4539
                     /gene="dnaX"
                     /locus_tag="PLI69_02365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOG41370.1"
                     /translation="MGQFIVSARKYRPNDFDSLIGQENIARTLKNSILRNQLAHAYLF
                     CGPRGVGKTSAARIFAKTINCSNISEDLVPCGICESCLSFAEGRSYSIHELDAASNNS
                     VDDIRNLTDKVRIPPQIGKYSVYIIDEVHMLSQQAFNAFLKTLEEPPSYAIFILATTE
                     KHKILPTILSRCQTYDFNRISVDNIVRNLKDIAQKEHVTIGDDTLHIIAQKADGAMRD
                     ALTLFDQTVAFCGHEVSYEQVIRNLNVLDYEYYFKLTDEFLKGDYAAAFLIFDEILSK
                     GFNALHFISGLSNHFRDLLVCKHDASQALLELAPSIAKRYRELSEKCSSDFLYEALAT
                     TTECEVSYRNSGNPRLLIEFALLRITRIGTAPSVAQPTATVQAAQPTVAKPAAVQATP
                     TATRDPEPGTQNPQLQTTDSKLQTPKTSLSIKDVMKEVKSGSSSKQMEFAQEIVKVAL
                     NEENLKQVWEELVSSQSRSPNLSSALAKGDPQIDLSRSVVSFSVSNITQKEYIDRHFL
                     KRLEAFLKERIDLPALRLEVTLNEVADQCSTSKLYMPSDKANYLQKTSEEYRELKKNL
                     GLDTI"
     gene            4558..5301
                     /gene="lptB"
                     /locus_tag="PLI69_02370"
     CDS             4558..5301
                     /gene="lptB"
                     /locus_tag="PLI69_02370"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOG41371.1"
                     /translation="MKLFCTNLVKKYGKRTVVKGVSIEVEQGEIVGLLGPNGAGKTTT
                     FYMITGLIKPNDGKIFLDEEEITKLPMYKRAQKGLGYLAQEASVFRTLSVEENLLSVM
                     EMADYSKEERYERLESLIEEFGLHKVRKNQGIQLSGGERRRCEIARALAIDPKFILLD
                     EPFAGVDPIAVEDIQHIIAKLKTKNIGIIITDHNVHETLAITDRAYLLYEGSILESGT
                     AEQLANNPEVRKKYLGQNFELRKAVLCDQ"
     gene            complement(5302..5577)
                     /locus_tag="PLI69_02375"
     CDS             complement(5302..5577)
                     /locus_tag="PLI69_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41372.1"
                     /translation="MEILIAALILVGFSVLVMSVSIIFKKNGKFPDGEISHNKTLREK
                     GIICAKEEELRLWGRKGKGMPDAGCPDGGCETCVFNSMHNPKGCSME"
     gene            complement(5579..6421)
                     /gene="nadE"
                     /locus_tag="PLI69_02380"
     CDS             complement(5579..6421)
                     /gene="nadE"
                     /locus_tag="PLI69_02380"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOG41373.1"
                     /translation="MILNDNIPIEVAYETIVTKMRNFFHATGMKKAVLGLSGGIDSAV
                     VMAIACEALGKENVHGLLMPSQFSTLHSVSDAVDLSNNLGIKYTIVPIEKIYNRFMRE
                     LFSFFEIGKWSVAQENLQARIRGTILMTYSNRFDALLLNTSNKSELFVGYGTLYGDLC
                     GAMMVIADLYKLQVYEIAGYINSSLGNVIPASTIQKTPSAELRPGQKDSDSLPPYDVL
                     DPIIYALHEEGKSAEEVIAEGADRVIVERIISLNRTYSFKLAQIPPVITVTDKPVAPN
                     YKCI"
     gene            complement(6435..7388)
                     /locus_tag="PLI69_02385"
     CDS             complement(6435..7388)
                     /locus_tag="PLI69_02385"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOG41374.1"
                     /translation="MVHSDHQFKIFSCRNSRYLAEKIAKNLHTELGKSEVTVFSDGEF
                     QPAFLESVRGATVFIVQSTFPPTENLFELLLMIDAARRASAYKVIAVIPYFGWARQDR
                     KDRPRVSIGAKLVANLLHAAGCDRVMTADLHADQIQGFFDFPVDHIYASNLFLSYIKG
                     LKLENLSIASPDMGGAKRANAYSRILSVPMIICHKSREKANVVGTMTAIGDVEDRNIV
                     IVDDIVDTAGTITKCADMLLDKGAISVRAVCTHAVLSGSACARVAKSGIKEFIVTDTI
                     PMRQDPETDTSKFTVLSIAETFADVIEKVYKFKSISDTFVF"
BASE COUNT         1982 a         1701 c         1677 g         2067 t
ORIGIN      
        1 accgttaaaa ttcgccacaa ttccgtttat actacagcct atcttcattt gtcaaagtat
       61 gcctccggcc taaaaatagg ctccaggggt tctcaggggc aggtaatagg ttatgttggt
      121 agcacaggta tgtctaccgg tccacatctt gattttaggg tttggaaaaa tggttcacca
      181 atcaatccgc ttaaaatgga gtctcctcct gccgagccta tacgggaaga acacaaagaa
      241 aaattcgagg caagtcgtga ctcgcttcgc caccttgcct cgaattatct tttggatgaa
      301 tactaccgta aatatgtgtt gaatctactc tgatttacac atcacttttt aagtgcatca
      361 cggatctctt tgagaatttt aacctcgtcg ctttcaggag ccggagtctc agcagctgct
      421 ttagctttgg cctcagcctc ttcttttgcc tttttggttt tctcagactc aaatttcttt
      481 gacatcttgt taataccctt aattacaagg aaaatggaga atgcgatgat aatgaagtta
      541 aacagcacgt tgataaattt gccatagctg acgtataggc cttccacggc ctcggtggtt
      601 tcggtcgctt ctacagcggc tcgcagggta attttccact ccgtgaaatc tattccgccg
      661 gtagcccagc tgatgagcgg catgataagg tcaccgacga gtgaagttac gatggctccg
      721 aatgctgcac cgataacaac accgattgcc atatccataa cgttaccctt cattgcgaat
      781 tctttgaatt cttttagaaa ttttgacata actgttgagt tttgttgagt tgatattgtt
      841 gttttagagt tttctgatga tgttgatacc atcacgcaaa ggtattatga cattttctac
      901 gcgtggatcc tctgacacaa tacgattgaa ttcactgata ccatgcgtct gagcatctgt
      961 tgttggggat tcttcataga ccttaccatc ccatagcaca ttatccgcca ggatgaagcc
     1021 cccactgcgg agcagcccta cggcagcttc aaaatagtga ggatattctc gcttattggc
     1081 atccaaaaat atcaggtcat aaccctcttc gagcgtagga agtacctgaa gcacatcccc
     1141 gaagatgagt ctgactctat cggtgatgcc cgccttttca tatccctcgc gtatgagatc
     1201 ttcgagttca tcgttgatct ctatggaatc gatcgtgccg cccggagcaa gtccgcgcga
     1261 aatcgctatt gtggagtagc ctgtaaaggt acctatctca agtactctgg agggggatat
     1321 cattttggta accatttcca gaaatttgcc cagaatagga cctgagagca ttctgccacg
     1381 gttggtacga aggtgcgtct gcctcgtgag ccattcaagt gcctctccct gagaggagga
     1441 atgtgcgata agatatctgc ccaaatggtc cattctcaat tacctgtata ttagagtcga
     1501 tagccgttcc ggagccagca cttcacaagc tacgcggcga atatcttcag cagtaatctc
     1561 ttctatcaat cgatggattt cgctgtcagg cattacattg ccgtaggata gcagactttt
     1621 gcccatcgat aatgcctgta cttcaccatt gtccgaagag atcgcatgct gaccgaggag
     1681 ttgcttttta gcagctttga gctgtgcttc agtcaatctg ccctcgcgga aataccctat
     1741 ctctttatct gtcaaagata ggcatttttc tacatctccc ttatcacatc cgaagtaaat
     1801 ggcgaaggtg ccggtatcgg agtactgggt gtaagaggat tccacagtgt agacgagagc
     1861 ggagcgttcc ctaaggctct ggttgaggcg agagttggag gaggggccgc caagcatatt
     1921 gcagagcaat agcagtgcaa gacgattctt gtcgtagagt gaataggcac tcgtacctaa
     1981 gatacaatgg gcttgatgat tcttgcgtga aacttcttta ttgaataagc tccctttatc
     2041 tagtgaagag ttgctttcgt agggggtcgg taaaggagtt tctatctctt tcggtccagt
     2101 gtagtggcgt gaaatggcct tttcaaccat atttacaact atttccggat ccatgtccga
     2161 aacagccgta aagcaaaggg ctgttggtat aaatctcgac ctggtatagt ttcgcaaatc
     2221 ttccgttgag atttttttaa gacttttggc atttccgaga atagacctgg agagaggatg
     2281 attgccaaag agcaactctt caaactcatc atatatcctc tcggcagggg agtccttgta
     2341 catattgatt tcatcaataa ttaccgagag ctccttgata agttccttct caggaaatgt
     2401 ggaactgagc actatttcaa aaagcagatc tactgactta ccgatatcct ctttgagagt
     2461 ggtagagtga actaccgtct cttctttggt tgtaaatgcg ttaagttcgc ctcccaggcg
     2521 ctccaggcgg ttgtttattt cgcgctggca gtgccttgac gtgcctttga agagcatgtg
     2581 ctccgcaaaa tgggcagtac ccgggagttc ttccggttcg tgacgtgtgc cggaacgtat
     2641 gctgagggca caatatgcca ccggagaagg agtccttttg aacgcaaatt ggataccgca
     2701 gggaagtgtt tgtatataaa tattttgtga ataattatcc atctattgtc gcttgagtgg
     2761 cgcaaagtta taaattattc ctatttttgt aaaatcactc aaaccctatc gtagagacat
     2821 aatatgggac agttcatagt atctgcacgc aaatacagac cgaatgattt cgattcgctt
     2881 attggacagg agaatatcgc caggacccta aagaattcca ttcttagaaa ccagttggcg
     2941 catgcatatc tattctgtgg tccacgcgga gttggaaaaa ccagtgctgc gaggattttt
     3001 gccaaaacca tcaactgctc caatatttcg gaagatttgg taccctgtgg tatttgtgag
     3061 tcctgcctct catttgccga aggacgttct tatagcattc atgaactgga cgctgcttcc
     3121 aacaactccg tagatgatat ccgcaacctt acagacaaag ttcgcattcc accacagata
     3181 ggcaaatata gtgtctatat catagacgag gtacacatgc tctcacagca ggctttcaat
     3241 gcctttctca agacacttga ggaaccaccc tcttatgcta tattcatcct tgctaccact
     3301 gaaaagcaca agatactccc caccatactc tccagatgtc agacttatga tttcaatcgg
     3361 atatcggtgg ataacatagt gcgtaatctt aaggatattg ctcaaaaaga gcatgtgacc
     3421 ataggggatg atactcttca tataattgct cagaaagcgg acggagctat gcgtgatgct
     3481 cttactcttt ttgaccagac ggtggctttt tgtggccatg aggtgagcta cgagcaggtt
     3541 atccgcaatc tcaacgtgct ggattatgaa tattatttta aactcaccga tgagtttctt
     3601 aaaggtgatt atgcggcggc attcctcatc tttgatgaaa tactttccaa aggattcaat
     3661 gctcttcact tcatatcggg tcttagcaat cattttcgcg acctgctagt ttgtaaacat
     3721 gatgcatcac aggcactcct tgagcttgct ccgtcgatag ccaagagata tagggaactt
     3781 tccgaaaaat gttcttcgga ctttctttat gaggcacttg caactactac tgaatgtgaa
     3841 gtctcctacc gaaacagtgg caatccgaga cttctgatcg aatttgcact gctcaggata
     3901 acccgtatag gcacagctcc ctctgttgca caaccaactg ccacagtaca ggcagcacaa
     3961 cccaccgtcg ctaagccggc cgcagtacag gccactccta cggcaacccg tgacccggaa
     4021 ccaggaactc agaacccaca actccaaact acagactcca aactacagac tccaaaaaca
     4081 tctctctcca taaaagatgt tatgaaagag gtaaaatcgg gatcatcctc aaaacagatg
     4141 gaatttgcgc aggagatagt caaggtggct cttaacgaag agaatctgaa gcaggtttgg
     4201 gaagaattgg tctccagtca gtcccgttct cccaatctct cttccgccct tgccaaggga
     4261 gatccgcaga ttgacctctc tcgttccgtt gtctcttttt cagtcagcaa cattacgcag
     4321 aaggagtata tagaccgtca tttccttaag cgtcttgaag catttcttaa ggagcgaata
     4381 gatctccctg cactacgtct cgaggttact ctcaacgagg tcgccgatca atgcagcact
     4441 tcaaaattgt atatgccttc cgataaggca aactatttgc agaagacatc cgaagagtac
     4501 cgcgaactca agaaaaatct cggacttgat accatttaga ataccgaaat atccgatatg
     4561 aagctttttt gtacaaatct ggttaaaaaa tatggtaaga gaactgttgt aaaaggtgtc
     4621 tctattgaag ttgaacaggg tgaaattgtg ggacttcttg gaccaaacgg ggccggaaag
     4681 actaccacat tctatatgat taccgggttg atcaagccca atgacggcaa aatttttctg
     4741 gatgaagagg agattacaaa gctacctatg tacaaaagag cgcagaaagg tctgggctat
     4801 ctcgctcagg aggcctccgt attccgtacc ctcagtgtcg aagagaatct tctgtcggtt
     4861 atggaaatgg cggactactc taaagaagag cggtatgaac gcctggagtc acttattgaa
     4921 gagtttggcc ttcataaggt gcgcaaaaat caagggatac aactttccgg aggtgaaaga
     4981 cgtcgctgtg agatagccag ggcgctcgcc atagatccta aattcattct tctcgacgag
     5041 ccttttgccg gtgtagatcc catagccgtt gaagacatcc agcacattat cgcaaaactc
     5101 aaaaccaaga acatcggtat aatcataacc gaccacaacg ttcacgaaac tcttgccatt
     5161 acagacagag cctatcttct ttatgaggga tcaattctgg aaagcggtac cgctgaacaa
     5221 ctggcaaata atcctgaagt tcgcaagaaa tacctggggc agaatttcga gcttcgcaaa
     5281 gcggtactct gcgaccagta attattccat agaacaccct ttcggattgt gcattgaatt
     5341 aaaaacacag gtttcgcaac ccccatcggg gcatccggcg tcaggcatac cttttccttt
     5401 acgcccccag agcctgagct cttcctcctt ggcacagatt atacccttct cccggagagt
     5461 tttattatgt gaaatttctc cgtcggggaa ttttccattc tttttgaaaa taattgagac
     5521 gctcatcacc aggactgaga atccaactaa aattaaggca gctatcaata tttccatatc
     5581 agatgcattt atagttagga gccaccggct tatccgtgac ggtgattaca gggggtatct
     5641 gcgcgagttt gaaagagtag gtacggttaa gtgaaataat acgttccaca ataactctgt
     5701 cagccccttc cgcaattact tcttctgcgc tcttgccctc ttcgtggaga gcgtagatga
     5761 tcgggtcaag cacatcatag ggaggcagcg aatcgctatc cttctgtccc ggcctgagtt
     5821 cagccgaagg tgttttctgt attgtggaag cagggattac attgcctagt gaagagttga
     5881 tgtagcctgc tatttcataa acttgcagtt tataaagatc ggcaatcacc atcattgcgc
     5941 cgcagagatc gccataaagg gtgccgtacc ctacaaataa ctcgctttta ttggaggtgt
     6001 tgagcagaag agcatcaaag cggttggaat aggtcatcag aattgtacct cggattcgtg
     6061 cctggagatt ttcctgcgca acactccatt tccctatctc aaaaaaggag aagagctccc
     6121 tcataaagcg gttgtagatc ttttcgattg ggacaatcgt atatttgatc cctaaattgt
     6181 tggaaaggtc tactgcatct gaaacggaat gtagggtgga aaactgagat ggcatcagca
     6241 gaccatgaac attttcctta ccgagcgctt cgcaagctat ggccatgaca acagccgaat
     6301 cgatacctcc ggagagtcca aggacggcct tcttcatgcc tgtagcatgg aaaaagttac
     6361 gcattttggt tactatcgtc tcgtaagcca cttcgatggg gatgttgtca tttagtatca
     6421 tatcattaag gtttctagaa tacaaatgtg tcgctgatgg acttgaactt gtagaccttt
     6481 tcgataacat cagcgaaagt ttcggctatg gaaaggacgg taaacttgga tgtgtcggtt
     6541 tccggatcct gcctcatggg gatggtgtcg gtaacgataa attcttttat gccggatttg
     6601 gcgacgcgag cacaggcact accggaaagc acagcatgtg tgcagactgc ccttacactt
     6661 atggcgcctt tatccagcag catatctgca catttggtta tggtacctgc cgtgtcgaca
     6721 atgtcgtcta caataactat atttctatcc tccacatctc ctatggccgt catagttccc
     6781 acaacattgg ccttttcgcg tgatttatgg caaatgatca tcggtacgct gagtatcctg
     6841 gaataggcat tcgccctctt ggcaccaccc atatccggag aggcgatgga gagattttcc
     6901 agtttcaggc ctttaatgta ggaaagaaaa agattgctgg catagatgtg atcaacgggg
     6961 aagtcaaaaa atccttgaat ttgatcagcg tgaagatcgg cggtcataac cctatcgcaa
     7021 ccggcggcgt ggagaagatt ggccaccagt tttgcaccga tggagactct cggacggtcc
     7081 tttcggtctt gtcttgccca accgaaatag gggattacgg caatcacctt ataggctgat
     7141 gcccttcttg ctgcatcaat cattagtaat agttcgaaca gattctcagt aggagggaat
     7201 gtcgactgga cgatgaagac tgtagctccg cgaacgcttt ccagaaatgc cggctgaaat
     7261 tctccgtcac taaatacggt tacttcagac ttgccaagct ccgtatgtag gtttttggca
     7321 attttttcgg ccagatagcg cgagtttctg caggagaaga ttttgaattg atgatcgcta
     7381 tgtaccatat gtttagtttt gagtttagag ttatgagtat gtagtgt
//
LOCUS       DAOSIE010000045         7422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9146_length_7422_cov_3.742365, whole genome shotgun sequence.
ACCESSION   DAOSIE010000045 DAOSIE010000000
VERSION     DAOSIE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9146_length_7422_cov_3.742365"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>537
                     /locus_tag="PLI69_02390"
     CDS             <1..>537
                     /locus_tag="PLI69_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41375.1"
                     /translation="GFESMTSTYWQRTNGSGVNLQFGAIVTPFPGFRLGATFTTPTWY
                     DMTDNWQRNMTSSFVNGNYYDVDSPLGAYSYKIKTPMRWSVGAAFTFLDRALISADYE
                     SADYSTIRMADDNGSKEVFSEENNEINRYFKRSDIVRVGAELWPLKIMGLRVGYNYYS
                     SPDPEFFDARHYISGGFGI"
     assembly_gap    538..547
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(692..1321)
                     /locus_tag="PLI69_02395"
     CDS             complement(692..1321)
                     /locus_tag="PLI69_02395"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOG41376.1"
                     /translation="MIRFDQSLLTTAYFPPVEYFFAIAGSGKVLIEQHEVYQKQSYRN
                     RCNIYSAAGKESLSIPILKESYQKVPIRDVRIDYGRPWLQQHKRAIVSAYNSSPFFEY
                     YIDDLFPILDRKDSFLFDLNLLLLEKCLELTGIRAKIEFTSEYRKEYPEGDFRSIIQP
                     KYRGDNLLVRYKKEKTWTQVFSDRFGFIGNLSIIDLLSNEGPNAISYIL"
     gene            complement(1314..2126)
                     /gene="lpxA"
                     /locus_tag="PLI69_02400"
     CDS             complement(1314..2126)
                     /gene="lpxA"
                     /locus_tag="PLI69_02400"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOG41377.1"
                     /translation="MINNNKYPGAYIHPDAKIAKNVEIGPFSYIAGKVEIGDGCHIGP
                     NATILDYVRMGENCKVFPGAVLGAEPQDLKFQGEESHVIIGNNTVIRECATINRGTAA
                     SGRYATVVGDNCLIMSYVHIAHDCRIGNNCIFSSYVGMAGETDVDDWAIVGGGTLIHQ
                     FSHIGTHVMIGGGTGVLKDVPPYILAARNPIHFEGINIVGLRRRGFTHDEIDEIREIY
                     RVIYDSDMNISDAVSRVRSAFPDTEVKKVILDFISNSKRGIVKKGASSSIDD"
     gene            complement(2123..3046)
                     /locus_tag="PLI69_02405"
     CDS             complement(2123..3046)
                     /locus_tag="PLI69_02405"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOG41378.1"
                     /translation="MKGYQKTLNNSHIFEGKGLHTGHHCRMTLFPAPTDHGIVFKRID
                     LGEDALVEASWRNVSGTARSTLLSKNGVEVMTIEHLLSALYGLGADNVLVEIDNMELP
                     ILDGSARPYVEALITDGLNVQDRPRRYLKVAEPFLFEDPETGASLQVLPAERFSVEVE
                     IDYGSKVLGVQKAKFTEGDDFASEIASCRTFCFLHELEFLLNHNLIKGGDMDNAIVIV
                     ENEVDDKTLSRLKSIFNVENLERVPEGYLNNLTLHFDNECARHKLLDLIGDFALAGLP
                     LKARVIARKPGHAINTAALKSMFEKNNIISS"
     gene            complement(3043..4032)
                     /gene="lpxD"
                     /locus_tag="PLI69_02410"
     CDS             complement(3043..4032)
                     /gene="lpxD"
                     /locus_tag="PLI69_02410"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOG41379.1"
                     /translation="MNISAQKIAEHLGGKIVGDPQVRVSAPARIEQGRPGTICFFANP
                     KYEHYIYTTKASILLVNSSFEPRQEISATLIKVDNAYESVALLLDLFNTLKKSRPGRN
                     RFFVRRPFSSKIGRGTYVGRGTFIGKKVKIGKNCHIYPQVYIGDGVTIGDNTIIYPGV
                     RIYRDSVIGQDVILHANVVIGSDGFGFAPREDGSYRKIPQTGNVIIEDDVEIGAGTTV
                     DRSTMGSTIIRRGVKLDNLCQIAHNVEVGDNTVIAALSGIAGSTKLGKGCILGGQSGI
                     VGHVTIADKTTLAAQSGIIGNVKNEGEALMGYPAISYRDYMRAYALFKASAKK"
     gene            4195..4683
                     /gene="rimP"
                     /locus_tag="PLI69_02415"
     CDS             4195..4683
                     /gene="rimP"
                     /locus_tag="PLI69_02415"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOG41380.1"
                     /translation="MIQKEKIEKILEEYFLKRSDLFLVEIKISRDNDIEIVVEIDRGF
                     LSLEECVNISRFVESKLDREVEDFSLTVGSAGLSRSFKVLRQYHKAVGSEIELKLKSG
                     EQLKGMLKAVSEEGITFVTKVSEKVEGRNKKVTREVVEEYAFDRIKSAKPVIKFDDYK
                     NR"
     gene            4709..5947
                     /gene="nusA"
                     /locus_tag="PLI69_02420"
     CDS             4709..5947
                     /gene="nusA"
                     /locus_tag="PLI69_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOG41381.1"
                     /translation="MEGLNLVSTFAEFKDQKNIDRATMMGVLEEVFLSQLKKMYSENS
                     DFDIIINIDKGDLQIFWNREVVEEVTDPMTEISFEDANKIDEYDLGEECSVEIKLSDF
                     GRRGILNLRQSLSGRIMDLERANLYNKYRTKVGDLFVGEVYQVWKREVVLRDDEGNDL
                     ILPKEEQIPGDFFRKGDTVRTIIKEVRFNASNAPVIILSRTSTDFLARLFEQEVAEIF
                     EGLITIKKIVRIPGERAKVAVESYDERIDPVGACVGVRGSRIHSIVRELRNENIDVIP
                     WTNNKSLLISRALSPAKISSMTFNEEAKQVNVYLKPSEVSLAVGKGGSNINLAGELVG
                     YKLEIYRDDDGQEPEEDVLLTEFSDEIDTWIIEQLQKIGCDTAKSVLALSVEDIVKRA
                     DLEEETAIEVQRILRAEFEE"
     gene            5977..>7422
                     /locus_tag="PLI69_02425"
     CDS             5977..>7422
                     /locus_tag="PLI69_02425"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HOG41382.1"
                     /translation="MSDQQQYRIKQVATAFNISTGTLLEFLEKKGQGSDLTITSKISQ
                     DVYDIVEKEFGHDKAIKEESKKIVSRVKAITDMDHRKEQQEEEPFEEVIIKSNVLTSP
                     EKQVKTPETPPAEPIPEKKPELPGINVIGHIDIDEIQPKTRSKQKQEKVEVAKQKPKP
                     EQKPEPEKAPVAPSEPEKKKKAPEKKSTPPLQEEKAKETKQVKSHIRKLPGLTIVGSI
                     DISQFEKKEKPKSEKSEKGEKGEQQGEKRNKGKRERIQNSKRVDVTKAHQDHKNQKEP
                     HVGGKGKYSKKDRFKSVRPEIDEDEVQKQIKETYARMTEGKGKTKGSRRRREKREIIS
                     QKAQEEQILQELSSNILKVTEFVTANELAILMNNTPVTKVIEACMNLGMMVSINQRLD
                     AETLVLVAEEFGYEIEFVTVELQGAIEQEEDKEEDMVERPPIVTVMGHVDHGKTSLLD
                     YIRKANVIAGEAGGITQHIGAYNVQLPSGRRI"
BASE COUNT         2269 a         1573 c         1697 g         1873 t
ORIGIN      
        1 ggatttgagt ctatgacttc cacttactgg cagcggacca atggctccgg tgtcaacctt
       61 cagtttggag ctatcgtcac tcccttcccc ggtttccgtc tgggtgctac atttactact
      121 ccaacctggt atgacatgac cgacaactgg cagagaaata tgacatcctc atttgtaaac
      181 ggcaattatt atgatgtgga ttccccttta ggagcataca gctacaagat taaaactccg
      241 atgagatgga gtgtaggagc tgcattcact ttcctggaca gggctcttat cagcgccgat
      301 tatgagagtg ccgactattc cactatccgg atggccgatg ataatggtag caaagaggta
      361 ttctctgagg agaataacga gattaacaga tacttcaaaa ggagtgatat agttagggtc
      421 ggagccgaat tgtggcctct aaaaatcatg gggctaaggg taggttacaa ttattacagt
      481 tcacctgacc ctgagttttt tgatgcacgg cattatattt ccggaggttt cggaattnnn
      541 nnnnnnngac cactctggat gtagcatacc ggagaagcct caacaattcc gagaacttta
      601 ctctttataa tgactatagt ggaatagaag cccctgaagg tacatatacc tacagcagag
      661 gtaaacttgt gtttacttta ggatttaaat tctaaagaat ataggagatg gcgttgggac
      721 cctcattcga cagaaggtcg atgatcgaga ggttcccgat gaaaccaaat ctatcggaga
      781 agacctgagt ccaggtcttc tcctttttat acctgaccag tagattgtca ccacggtact
      841 ttggctgtat tatgctgcgg aaatcccctt cgggatactc tttccgatat tcggaggtaa
      901 actcaatctt tgctcttata ccggtcagtt cgagacattt ttccaacaaa agcaggttga
      961 gatcaaaaag aaaactatct tttctatcga ggataggaaa aaggtcgtcg atatagtact
     1021 cgaaaaatgg tgatgagtta taagctgaga caattgccct cttatgttgc tggagccacg
     1081 gtctgccata atctatcctg acatcacgaa taggcacctt ttgataactc tccttgagta
     1141 tcggtattga aagagattct tttcctgcag ccgaatagat attacaacgg ttgcgatagg
     1201 actgcttctg gtagacttca tgctgctcga taagtacctt tccgctacct gcaatagcaa
     1261 aaaaatactc caccggaggg aagtatgcgg tagttaagag ggattggtca aacctaatca
     1321 tcaatagaac tgcttgcacc tttcttgacg atgccccttt tggagttgga tatgaaatcc
     1381 aaaataacct ttttgacttc ggtatccggg aaggccgacc tcactctgga aacagcatca
     1441 gagatattca tgtccgagtc atatattact ctgtaaatct cacgaatttc atcaatctcg
     1501 tcatgagtaa atcccctacg gcgcaggcct acaatattga ttccctcaaa atggatagga
     1561 ttgcgggcag caagtatata gggaggaaca tccttcaaca ctcccgtacc acctcctatc
     1621 atcacatggg ttccgatatg ggaaaattgg tggattagcg tacctccgcc tactattgcc
     1681 caatcatcca catcggtctc ccctgccatt cccacataac tcgagaatat gcagttgtta
     1741 cctatacggc aatcgtgcgc aatgtggaca taggacatga taaggcagtt atcaccaaca
     1801 acggtagcat atctgccgct ggcagcagta ccgcgattga tggtagcaca ctctcttatt
     1861 acggtattgt tgccgattat aacgtgactc tcctccccct gaaacttgag atcctgtggc
     1921 tcagctccaa gtactgcccc gggaaagact ttacagtttt cacccattct gacataatca
     1981 agaatggtgg cattggggcc tatatgacac ccatctccta tttcgacctt tccggctata
     2041 tagctgaatg gccctatctc tacattttta gcaattttgg catccggatg gatatatgca
     2101 ccggggtatt tattgttgtt gatcatgatg atataatgtt atttttttca aacatggatt
     2161 taagagcggc agtattgatg gcgtggcccg gtttgcgggc aatcactctt gcttttagag
     2221 gaagtcctgc gagggcaaaa tcccctatta ggtcaaggag tttgtggcga gcgcattcgt
     2281 tgtcaaaatg gagggtcaga ttgttgagat acccttcagg aaccctttca aggttttcaa
     2341 cattgaaaat gctctttaga cggctcagag tcttgtcatc cacttcgttt tccacgatca
     2401 ctatggcatt gtccatatca cctcccttga taaggttgtg attgagtagg aattccaatt
     2461 cgtgaagaaa gcagaatgtt cggcaagagg ctatttcaga agcgaaatca tcaccttcgg
     2521 tgaatttagc tttctgcaca cccaatactt ttgagccata gtcaatctcc acttcgacgc
     2581 tgaacctctc agcgggaagc acctgaaggg atgcgcctgt ttccgggtct tcaaataaaa
     2641 agggttcggc aactttaaga tatcttctcg gtctatcctg cacgttcaaa ccatccgtta
     2701 tcaaggcctc gacatagggc cttgcgctgc cgtccagaat tggtagctcc atattgtcta
     2761 tttccaccag aacattatca gccccaagcc cgtaaagcgc tgaaagcaga tgttcgattg
     2821 tcattacttc gaccccattc ttgctcagga gtgtacttct ggcggttcct gagacattac
     2881 gccaagaagc ctcaacaaga gcatcctcac cgaggtctat tcttttgaat actattccat
     2941 gatcagtagg agcgggaaag agagtcatcc tgcaatgatg accggtatgc aatcccttcc
     3001 cttcaaatat gtgactgtta tttaatgtct tctgatatcc cttcatttct ttgcacttgc
     3061 tttgaacaag gcataagccc tcatataatc tctgtaactt atggccggat agcccataag
     3121 agcttctccc tcgtttttca catttccaat gatgccgctc tgagcagcga gggttgtctt
     3181 gtcagcgatg gttacatggc cgacaatacc actctgtcca cctaaaatac atcctttccc
     3241 aagttttgtc gaaccggcaa taccgcttag agccgctata actgtattgt caccaacttc
     3301 tacattgtgt gcaatctgac aaagattgtc aagcttaact ccacgtctga ttatggtaga
     3361 gcccattgtc gacctgtcaa cagttgtgcc ggcacctatc tccacatcat cctctatgat
     3421 cacatttcct gtctggggta tcttgcggta tgagccatct tcgcggggag caaagccaaa
     3481 tccatcggaa ccgataacca catttgcatg aaggatgaca tcttgtccga taacgctatc
     3541 acgataaata cgcacacccg gataaatgat ggtattgtca cctattgtta ctccgtcacc
     3601 aatatagacc tgcgggtaga tgtgacaatt tttccctatc ttaacttttt tgcctataaa
     3661 agtaccacgt cccacataag taccgcgccc tattttagat gaaaaggggc gacgaacaaa
     3721 aaagcgattt ctcccgggac gacttttctt tagagtatta aaaaggtcaa gaagaagtgc
     3781 tacggactca taggcattat ctacctttat aagggttgca gaaatctcct ggcgaggctc
     3841 aaaagagcta tttaccagca ggatcgaagc ttttgtggtg tagatataat gttcgtattt
     3901 gggattggca aagaagcaga tggtgcccgg gcggccctgc tcaatccttg caggagcaga
     3961 gactctgacc tgcggatccc caactatctt tccccccagg tgttcggcta tcttttgtgc
     4021 agaaatattc atagataatt tagtttcatt aataatcccc tatattcatt cataaaatat
     4081 gccaaaaaga taacaaaatt ttgccgtttg atgtagaatt gctaactttg cgccagatat
     4141 agtggaagag ataggggacg tgtagtcccc ttatttttta aaaatatcca aaggatgata
     4201 caaaaggaga agatagaaaa gattttagaa gagtatttcc tgaaaaggag cgatctgttt
     4261 ttagtagaga tcaagattag cagggataat gatatagaga tcgttgtaga aattgaccga
     4321 ggatttctct ctctggagga gtgcgtaaat atcagccgtt ttgtagaatc caaacttgac
     4381 agggaggttg aagatttttc gcttacagta ggttctgcag gtctttcaag gtcttttaaa
     4441 gtactccgac agtatcacaa agcagtgggt tccgagatcg aactcaaact aaaaagcgga
     4501 gaacaactta aaggtatgct gaaagcggta tcggaagagg gcatcacttt cgtaacgaaa
     4561 gtttctgaga aagttgaagg tcggaataag aaagtgacca gggaggtggt agaagagtac
     4621 gcttttgacc ggataaaatc ggcaaaaccc gtcattaagt ttgatgatta taaaaacaga
     4681 taaaataaca actatttaac gataaaaaat ggaaggtttg aacctagtca gcacatttgc
     4741 tgaattcaaa gatcagaaaa atatagatag ggccaccatg atgggcgtac tagaggaggt
     4801 attcctctca caactaaaga agatgtatag cgaaaacagt gacttcgaca tcatcatcaa
     4861 catcgataag ggtgacctcc agattttctg gaacagggag gtagtggaag aggttaccga
     4921 ccccatgacg gagatctctt tcgaggatgc caacaagatt gacgagtatg acctcggaga
     4981 ggagtgttct gtggaaatta aactctcaga ctttggtcgc aggggaattc tcaacctcag
     5041 acagagtctt tcaggacgaa tcatggacct tgagagggcc aacctttaca acaagtatcg
     5101 cacaaaagtg ggtgatctct tcgtaggaga ggtctaccag gtatggaaga gagaggttgt
     5161 actaagggat gatgaaggta acgatctgat tcttccaaaa gaggagcaga tcccgggtga
     5221 tttctttaga aaaggtgata ccgtaagaac catcataaaa gaggtaagat tcaacgcgag
     5281 caacgccccc gtaatcatac tttcacgtac atctactgac ttccttgcaa gacttttcga
     5341 gcaggaggtt gcagaaattt tcgaaggtct gattaccatc aaaaaaatcg taagaatacc
     5401 cggcgaaaga gccaaggttg ccgtggagtc atacgacgag cgcattgacc ctgttggagc
     5461 ctgcgtaggc gtaagaggta gccgtattca cagtatagta cgtgagttgc gcaatgaaaa
     5521 tatagatgtt ataccttgga cgaacaacaa atcactactc ataagcagag ctctaagtcc
     5581 tgcaaaaatc tcttcaatga cattcaacga agaggcaaaa caggtgaatg tctatctaaa
     5641 accttccgaa gtttccctcg cagtaggcaa gggtggcagc aatattaacc ttgcaggtga
     5701 acttgtaggc tacaaacttg agatttaccg cgatgatgac ggacaagagc cggaagaaga
     5761 cgtattgctc actgaattca gcgacgaaat cgatacctgg atcatagagc aattacagaa
     5821 aataggatgt gatacagcca aaagcgtttt ggcactttca gttgaagata tcgtaaagag
     5881 ggctgacctt gaagaagaga ctgcaatcga ggtacaaaga atccttagag cagaatttga
     5941 agagtagttt taacaattaa tatcgggaga aattatatgt cggatcagca acaatacaga
     6001 ataaaacagg tagctacggc ctttaacatt agcacaggta cactgctcga attccttgaa
     6061 aagaaaggac aaggaagcga cctgactatc acgtctaaaa tttcgcagga tgtctatgac
     6121 atcgttgaga aggagtttgg acatgataag gcgatcaagg aggaatcgaa gaagattgta
     6181 agtagggtta aggcaataac tgatatggat caccgcaagg agcaacagga agaggagcct
     6241 tttgaagagg tcataataaa gagcaatgtt ctgactagcc ctgagaaaca ggttaaaact
     6301 cccgaaacac cccctgctga accaatacct gagaaaaagc cggaacttcc gggtataaat
     6361 gtcataggac atatagacat tgacgagata cagcctaaaa cccgttcaaa acagaaacag
     6421 gaaaaggtag aagtagcgaa acagaagccg aagcctgagc aaaagccgga acccgaaaag
     6481 gctcctgtag caccatccga acctgagaag aagaaaaaag ctccggagaa aaagagtaca
     6541 ccccccttac aagaagagaa agccaaagag acaaaacagg taaagagtca tatccgcaaa
     6601 cttccgggct taacgatagt aggttccata gatatctctc aatttgagaa aaaagagaag
     6661 ccaaaaagtg aaaagagtga aaagggagaa aagggagagc agcaaggtga gaagaggaat
     6721 aaaggtaaga gagagcgtat ccaaaacagc aaaagagtgg atgttaccaa agctcatcag
     6781 gatcataaaa atcaaaagga gcctcatgtc ggaggtaagg gcaagtatag caaaaaggat
     6841 cgcttcaaat cagtacgtcc tgaaattgat gaggatgagg tacaaaagca gatcaaagag
     6901 acctatgccc gcatgaccga aggcaaagga aagaccaagg ggtcaagacg tcgccgtgaa
     6961 aagagggaaa taatttccca gaaggcacaa gaggaacaaa tacttcagga actctccagc
     7021 aatatcctca aggtaaccga gtttgttaca gccaacgaac ttgcaatcct gatgaacaat
     7081 acccctgtta caaaggttat agaggcctgt atgaacctcg gaatgatggt ttccatcaac
     7141 cagcgcctgg acgcagagac actcgtactt gtggcagagg aattcggata tgagatagag
     7201 tttgtgactg tagaactaca gggtgccatt gagcaggagg aagacaaaga ggaagatatg
     7261 gttgagcgtc cgcccatcgt taccgtgatg gggcacgtgg accatggaaa aacctcacta
     7321 ctggactata tccgcaaggc taatgtaatt gccggagagg caggtggcat tacgcagcac
     7381 ataggtgctt acaacgtaca gctaccttca ggaagacgaa tc
//
LOCUS       DAOSIE010000046         7297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9332_length_7297_cov_3.487434, whole genome shotgun sequence.
ACCESSION   DAOSIE010000046 DAOSIE010000000
VERSION     DAOSIE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9332_length_7297_cov_3.487434"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLI69_02430"
     CDS             <1..428
                     /locus_tag="PLI69_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41383.1"
                     /translation="TLDECGDPDLKDIYDTLKDDPAVNSRVMFYIRPMRRHYYPTEKE
                     LEEYSLLNSPDHPLKEKKFTVKRERNGCNAHALDEMFKLLQNETDQEFRVAIAEVFGW
                     YVYSYKKDEIISRCEELLANEKDERVANELLKSIKRLKR"
     gene            477..3248
                     /locus_tag="PLI69_02435"
     CDS             477..3248
                     /locus_tag="PLI69_02435"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG41384.1"
                     /translation="MQRIQKVLMLCLLFASVVAFAQTRGASGTSVTGKVMEQGTKVPV
                     EFAVVILYPSETYTTTDKDGNFEFKRVDAGKSTIHVQFVGMKTIEQVIEIVPGKENVF
                     NFEMQVENFRLDEVQVVATQSKAGAATSSNISRQAMDHLQTSSLNDVMQLLPGVAITN
                     PYLSSANYITIRNATSIGTSIIVDGSPLSSNANMQALAPSITGGAGGTMSGIDSRMVS
                     TDNIESIEVIRGIPSVEYGDLNSGAVVIRSKAGKEPLSIRLKANPSTYQAAASVGFKL
                     GENAGHLNLSGDYAYNTTRLYEAYSYYQRFNAKALWSKKFGSNWNTNTTLDLGISKDT
                     RDLNPDDKRTMLVTKGDSKSVRLNTNGTVNVNKGWLNNITYAAAISYADKYNYYNQLL
                     SNAESLHTVCEYDGAILSNVAGQDVYDITGKKITNIPASEAKNWIKVLPYEYVQTYEI
                     LGKELNAYAQIKANFNKRWGNINNHILVGADYKTDGNLGEGKVYDDETPPFRNNASDY
                     SSYRKRPYYDVPFVHQLGLFIEDTYKHTFGRNELSIKAGARFDLINGKTAISPRINAS
                     MEIIPDILTIRGGWGNTAKAPTALMLYPERAFFNYNNYDFQDGTFRQTVATVRVFDTS
                     NPDLEIAKMRKAEVGIDLKLFDQYRLSITGYDELTNNGYTLGKDLSSYRLIEFVKYKA
                     VKNETGGYSPEYEGTYNIFSTFAKPMNNHYRHNRGIEFELDLGRFDAIRTSVFLNGAW
                     NQYTARSNGYTFDQNSRGNEIESNVAIYERGLTTERTDNLITTLRLTHNIPEIGFVIT
                     LATQVNWFEKFWTEYGNDTMFIKYLSYKDGKVYDFDPSKKDDPEFAYMFDAKNENRFI
                     VEQYFPTVTFNLNISKEIGDRLTASFFVNNMFNSRPLYESKRTPGSFTELLSDNRLFF
                     GFDLKVNIF"
     gene            3264..4472
                     /locus_tag="PLI69_02440"
     CDS             3264..4472
                     /locus_tag="PLI69_02440"
                     /inference="COORDINATES: protein motif:HMM:NF027540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4876 domain-containing protein"
                     /protein_id="HOG41385.1"
                     /translation="MKKYFLLAIVLLTALSCEQFKQVAEIVEVTPISATVKISVSGAD
                     SDIPLPASYTVRFINYGENFEISRSTDASGNVSADGLIPGVYTVTATAESAGGGFTYY
                     FSGSLTNINILTGGQTFNIEVVSSRAGALIFKEIYFNGSKGYYFKDQFYEIYNNSDAV
                     QYVDGMGIGNILPALASTTTYNWEVPDGKPENYIYLASIWQVPGDGDDFPVKPGESII
                     IAQIAQNHTAIKPTSPVDLSGAEFETFIVKQSVNPDNPNAHNMILAFDKGVFGTQWLV
                     TVFGGAYAIFFPDSEIDPNGWVKPQGLSNQAYPIPIDCVVDAVEIVNNATKINQKRMP
                     TALDAGATYTGATYNSNSVSRKIRETTKDGRHIYMDTNNSSDDFQINNEAMVRRNNPG
                     VPSWNTWYKK"
     gene            4499..6118
                     /locus_tag="PLI69_02445"
     CDS             4499..6118
                     /locus_tag="PLI69_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41386.1"
                     /translation="MKKYIIASVLALSTILIAQAQGSKSTIYPTVLEINQAKATWFNT
                     NNAAGMILTPLSDFGTLSVGYDMKVGSYRQQQEGDLKTLNINTSGSSPLGSARVWGEF
                     SYQNITQENSKFNTLRLTLEPDMPYILADSRPSYFKKQVYDMTVKAATPLLWDVVAFG
                     VTANYFTKSGAKQIDPRSDSYEYGIEVKPSTVLAFGDHSAGLTFIYKNAFDRYSPLNS
                     NSQQDQPAFNLRGVGSFVNAVVGSFGGLGTYYYKTNRLGGALQYGYQGNNLKVLAEAN
                     YTYQVIDVFHTPAKPFRMGSTIQQVMGGNIQMVLEGDYFTNKVTIDGYKRSTDGIEYI
                     QVIDATYTVQQWVSIAKFIRSEYDFTGLSLKYDIFRDSNEGYDWMAGIQGQYTDRLDT
                     YIIPESSFKAENVYGEIYGKKNFMSGNNSIVVGLSAGYNYNIAGEHKYNGPFPDSEVV
                     RDLYEKDLAILSANYYQFGINLKAIFPFGEKNTIYVAASCKYLRANETLHNYSYGTDT
                     EGKNFWWNLNPGNQLTPSARTFANFSIGFTF"
     gene            6143..>7297
                     /locus_tag="PLI69_02450"
     CDS             6143..>7297
                     /locus_tag="PLI69_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41387.1"
                     /translation="MKKISFLFFVLLCLPLGAQQNIISPTQSVRNSFAIFVDQDTYDA
                     CKEEIHAYRYLIEKDGLATYILSAEWQSPEHVKFFLKKYYDEQKLEGAVFVGNIPVPM
                     VRGAQHFTSAFKMDQEAYDYYDSSVPSDRFYDDFDLKFRFLHRDSTHKHLYYYWLTGD
                     SPQRISCDIYTGRIRSTAKGEAGFTQIRNYLRKVVKERSVENPLDVVVQYTGDGSFSN
                     SLIAWKDQSYVMGEQVPAALQGHADKARFYLFAMYPYMKEILTEEMKRDDVDLMLFHE
                     HGVTERQYLTGAIPNEYEEDYFTSGKRLLRRQLRSVEKRGNSVQEAMDRYIREHGIDS
                     TWFEGWNDPAVIAEDSLEYAKTGFELEDIRTIGPNPRVVILDACYNGDFRN"
BASE COUNT         2146 a         1741 c         1619 g         1791 t
ORIGIN      
        1 agacgctcga cgagtgcggt gatcctgacc ttaaggatat ttatgatact ctcaaggatg
       61 accctgctgt caactcaagg gtgatgttct acataaggcc gatgaggaga cattattatc
      121 ctactgaaaa ggaacttgag gagtactcgc tgctgaactc tcccgatcat ccgctcaagg
      181 agaagaagtt taccgtcaag cgagagcgca acggttgtaa tgcccacgct ctggatgaga
      241 tgttcaagct tttacaaaat gagaccgacc aagagttcag ggtagctatt gccgaggttt
      301 tcgggtggta tgtctattcc tataaaaaag atgaaataat cagtaggtgt gaagagctgc
      361 tcgcaaatga aaaagatgag cgtgtggcca acgagctcct caagagcatt aagcgtctaa
      421 aaagataaaa ttaaacaaac atacaaatta ttattaaccc taattcaaca caacttatgc
      481 aaagaattca gaaagttctc atgctctgcc tgttgttcgc aagtgtggta gccttcgctc
      541 aaacgagggg ggcttccggc acatcggtaa ccggcaaagt gatggaacaa ggcactaagg
      601 tgcccgtaga atttgccgtg gttatactgt atccttcgga gacatatacc actacagaca
      661 aggacggcaa ctttgaattc aagagagtgg atgcaggtaa atccactatc catgttcaat
      721 ttgttggaat gaagaccatc gagcaggtga ttgaaattgt ccccggcaaa gaaaacgtct
      781 tcaactttga aatgcaagta gagaacttcc gcctcgacga ggtgcaggtt gtggccactc
      841 agagtaaagc gggagcagcc acatcttcca acatttcaag acaggcaatg gaccacctgc
      901 aaaccagttc tctaaatgat gtaatgcagc tgctgccggg tgtggcgatt accaatccct
      961 acctttcaag cgccaactac atcaccattc gtaacgcgac gagtattggt acatccatta
     1021 tcgtggatgg atctcctctc tcttccaatg ccaacatgca ggcccttgca ccatccataa
     1081 caggtggagc tggcggaact atgtccggta tcgactcacg tatggtctcc acagacaata
     1141 tcgagtccat cgaagtcatc aggggcatac cctccgtaga gtacggtgac ctcaactcag
     1201 gtgccgtagt tatccgttca aaggcgggta aagagccttt gagcattcgt ctcaaagcca
     1261 atcccagcac ctatcaggca gctgcatcag taggcttcaa acttggagag aatgccggtc
     1321 atctcaacct ttccggtgac tatgcttata atactaccag actttacgag gcatactctt
     1381 actaccagag gttcaacgca aaggctctgt ggtcgaagaa atttggctcc aactggaaca
     1441 ccaatactac tctcgacctg ggtattagca aggatacaag agatttaaac cccgatgaca
     1501 agcgtacgat gctcgttacc aaaggtgact caaaaagtgt cagattgaac accaacggta
     1561 ccgtaaacgt aaataaagga tggttaaaca acattactta tgccgctgca ataagctacg
     1621 ccgataagta caactactac aaccaactcc tttccaacgc agaatcactt cacacggtat
     1681 gtgagtatga cggtgccatc ctttccaatg tggccggtca ggatgtttat gacataaccg
     1741 gcaagaagat taccaacata cctgcaagcg aagccaaaaa ctggatcaag gtactcccat
     1801 acgaatatgt tcagacctat gaaatcctcg gtaaggagct caatgcctac gctcagatca
     1861 aggccaactt caacaagagg tggggaaaca tcaacaacca catcctcgtg ggtgcagact
     1921 acaagacaga cggtaacctc ggtgaaggaa aggtctatga cgatgagact cctcctttca
     1981 gaaataacgc cagtgactac tcttcttacc gcaagagacc ctattatgac gtgcctttcg
     2041 ttcatcaact cggtctcttc attgaagata cttacaagca tactttcgga agaaatgagc
     2101 taagcattaa agccggcgcg cgttttgacc tgattaacgg caaaacagcc atttcgcccc
     2161 gcatcaatgc atcgatggag atcattcccg atattctgac aatacgcgga ggatggggaa
     2221 atacggcaaa agctccgact gccctgatgc tctatcccga gagggctttc ttcaactaca
     2281 acaactatga cttccaggat ggtaccttca gacaaacggt agctaccgtc agagtatttg
     2341 acacaagcaa tccggacctg gagatagcca agatgcgcaa agccgaagtt ggtatcgacc
     2401 ttaagctctt tgatcagtat cgtctctcca ttaccggtta tgatgagctg accaacaatg
     2461 gttataccct cggtaaagat cttagcagtt acagacttat cgaatttgta aaatataagg
     2521 ctgtcaagaa tgaaacaggt ggatactctc ccgagtatga aggcacctac aatatctttt
     2581 caacctttgc aaagccgatg aacaaccact acagacacaa cagaggtata gagtttgaac
     2641 tggatctcgg ccgttttgac gccatcagga catccgtatt cctcaatggt gcctggaacc
     2701 aatacacggc aagaagcaac ggttatacat ttgaccagaa tagtagaggt aacgaaatag
     2761 agagcaatgt ggcaatctat gagagaggcc tcactactga acgtaccgac aacctgatta
     2821 ctacactccg tcttacacac aatatacccg agataggatt tgtgatcaca cttgctaccc
     2881 aggtcaactg gtttgagaag ttctggacgg aatatggcaa tgacaccatg ttcataaagt
     2941 atctatccta caaggatggc aaggtctatg actttgatcc ttccaagaag gatgatccgg
     3001 aatttgcata tatgttcgac gcaaagaacg agaatagatt cattgtggag cagtatttcc
     3061 ccacagtgac cttcaatctc aatatttcaa aggaaatcgg tgacagactt accgcatcat
     3121 tctttgtaaa caatatgttc aacagtcgtc ctctctacga gtccaagaga actcccggtt
     3181 catttaccga gcttctcagt gacaacagat tgttcttcgg tttcgatctt aaagttaaca
     3241 ttttctaaac ggaggatagg attatgaaaa aatacttttt attagcaata gtactcttga
     3301 cagccctctc ctgtgagcag ttcaagcaag tagcggagat cgtggaggtg actcccatca
     3361 gtgcaactgt caagatttcc gtcagtggcg ctgatagtga cataccactt ccggcaagct
     3421 acactgtacg ttttatcaac tatggtgaaa atttcgagat ttcaaggtcc acagatgctt
     3481 ccggcaacgt aagcgccgat ggtctgattc ccggagttta tacggtaact gcaaccgctg
     3541 aatcagcggg tggcggattt acctactatt tcagcggtag tctgaccaac atcaatatcc
     3601 tcaccggagg acaaaccttc aatatcgaag ttgtctcatc cagagcaggt gcgcttatat
     3661 tcaaagagat ctacttcaac ggctctaaag gatattattt caaggaccag ttctacgaga
     3721 tctacaacaa tagcgatgca gtacagtatg tggatggcat gggcataggt aatatcttac
     3781 ccgccctggc ttcaacaacc acctacaact gggaagttcc tgatggcaaa cccgagaact
     3841 acatctattt ggcctctatc tggcaagtgc cgggtgatgg agacgacttc cctgtaaaac
     3901 ccggtgagtc catcatcatt gcacagattg cacagaacca caccgccatc aaaccgacca
     3961 gtccggtgga cctcagcggt gctgaattcg agactttcat cgtcaagcag agtgtcaacc
     4021 ccgataaccc caatgcacac aacatgattc ttgcatttga caagggagtc ttcggcacac
     4081 agtggcttgt tacagtgttc ggaggtgctt atgccatctt cttcccggac tccgagattg
     4141 accccaatgg ctgggttaaa cctcagggct tgagcaacca ggcatacccg ataccaatcg
     4201 actgcgttgt tgatgccgtt gaaatagtaa acaatgctac caagatcaac cagaaacgta
     4261 tgcctaccgc tcttgatgcc ggtgcaacct acacgggtgc cacttacaac agcaatagcg
     4321 tcagcagaaa gattcgtgaa actacaaagg atgggcgtca catatatatg gacaccaaca
     4381 actcttccga tgatttccag atcaacaatg aagctatggt acgtcgtaac aatccgggag
     4441 ttccctcctg gaatacatgg tataagaaat aatttactga cctctaacaa tgaaaaagat
     4501 gaaaaaatat attatcgcat cagttttggc tttgagtaca atattgattg ctcaggcgca
     4561 ggggagtaaa tctaccatct atccgacagt gctggaaata aatcaggcta aagccacatg
     4621 gttcaatacg aacaatgcgg caggaatgat actgactcct ctgagtgact tcggtacact
     4681 ctcggtaggc tatgacatga aagtaggctc ctacaggcaa cagcaggagg gagacctcaa
     4741 aactctcaat atcaacacat ctggttcgag tcctctcggc agtgcacgtg tatggggtga
     4801 attctcttat cagaacataa ctcaggagaa ctccaaattc aataccctcc gccttaccct
     4861 cgaacccgat atgccttaca ttcttgcaga ctcccgtccg agttatttca agaaacaggt
     4921 ttacgatatg actgtaaagg ctgccactcc gcttctttgg gacgtagtcg cttttggcgt
     4981 aaccgccaac tacttcacca agagcggtgc aaagcagatt gacccccgaa gcgattcata
     5041 cgaatatggc attgaagtaa agccctccac cgtactcgca tttggtgatc acagtgcagg
     5101 tctgacattc atttataaga atgctttcga caggtactcc cctctcaaca gcaacagcca
     5161 gcaagatcag cccgcattta acctaagggg tgtcggctcc tttgttaacg cggttgtggg
     5221 ctcatttgga ggactaggta cctattacta caagaccaac cgactcggcg gggctctcca
     5281 gtacggctat cagggaaata atctcaaggt tctcgcagaa gccaattaca cctatcaggt
     5341 aatagatgta ttccacacgc ctgccaagcc cttcagaatg ggatcaacca ttcagcaggt
     5401 tatgggaggc aatatccaga tggtactcga aggcgattat ttcaccaata aggttacaat
     5461 agatggctat aagaggagca ctgacggtat cgaatacata caggtaatcg acgccacgta
     5521 cacggtacag cagtgggttt caattgccaa atttatccgt agcgagtatg acttcacggg
     5581 tctttcccta aagtacgata ttttcaggga tagcaacgag ggttatgact ggatggcggg
     5641 tatccagggc cagtatactg acagattgga cacctatatc atccccgaat cctccttcaa
     5701 ggctgaaaat gtttatggag agatttacgg caagaagaat tttatgtccg gaaataactc
     5761 aatcgtagtg ggcctgagcg ctggttataa ttataatatt gcaggtgagc ataaatataa
     5821 tggtcctttc cccgattctg aggtagtcag agacctatat gagaaagatc ttgcaatcct
     5881 tagcgccaac tattaccagt tcggcatcaa tcttaaggca atctttccct ttggtgagaa
     5941 aaacaccatt tatgtagctg caagttgtaa gtatctccgt gctaatgaga cacttcacaa
     6001 ctatagctat ggcaccgaca ctgagggcaa gaatttctgg tggaacctca accccggaaa
     6061 ccaattaacc cccagcgcaa gaacctttgc taatttttca atcggattta ctttctaata
     6121 ataaagtatc cagcaccaat aaatgaaaaa aatatcattc ctcttttttg ttttgctgtg
     6181 cctccctttg ggggcgcagc aaaatattat ttccccaacc caaagcgttc gcaactcgtt
     6241 cgctatattt gtagatcagg acacctatga tgcctgcaag gaggagatac acgcctatcg
     6301 ctacctcata gagaaagatg gcctcgctac ctatattctc tctgctgaat ggcaatcccc
     6361 tgagcatgta aaatttttcc taaaaaaata ttacgatgaa cagaaacttg agggagcggt
     6421 attcgtgggc aatattccag ttccaatggt tcgcggagcg caacacttca cctcagcatt
     6481 caaaatggat caggaggctt atgattacta cgactcatcc gtcccttcgg atcgtttcta
     6541 tgatgatttt gacctgaagt tcagatttct tcatcgggat tccacccata aacatctcta
     6601 ttactactgg ctgaccggtg attcacccca acgcatctcc tgtgacatct acacaggacg
     6661 tatacgctcc acagccaagg gtgaagcggg cttcactcag atccgcaact acctcagaaa
     6721 ggtagtaaaa gagagaagcg ttgagaatcc ccttgacgta gtggtacaat acaccggaga
     6781 cggttccttc tctaacagtc taatcgcctg gaaggatcag agctatgtga tgggagagca
     6841 agtgccggca gctttgcagg gccatgccga caaagccagg ttttatctct tcgcaatgta
     6901 cccctatatg aaggagattc tgaccgagga gatgaagcgt gacgatgtag acctgatgct
     6961 ctttcacgaa catggtgtga ccgagaggca gtatctgacc ggcgctattc caaatgaata
     7021 tgaagaggat tattttacct ccggtaaaag attgcttcgc agacagctta gaagtgtgga
     7081 aaaaagaggt aacagcgtgc aggaggctat ggataggtat atcagggagc atggtatcga
     7141 cagcacctgg tttgagggtt ggaatgaccc tgcagtaatt gcagaagact ctctcgagta
     7201 tgctaagacg ggctttgaac ttgaagacat ccggacaatc ggtccaaatc cccgcgttgt
     7261 gatacttgac gcctgctaca atggagactt caggaac
//
LOCUS       DAOSIE010000047         7205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9451_length_7205_cov_3.980280, whole genome shotgun sequence.
ACCESSION   DAOSIE010000047 DAOSIE010000000
VERSION     DAOSIE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9451_length_7205_cov_3.980280"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLI69_02455"
     CDS             complement(<1..358)
                     /locus_tag="PLI69_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOG41388.1"
                     /translation="MKKRTKKVTTKDVIKYLMMLALAGFLLYFAFRNISWDEFVAGLR
                     SCNYWWILLSMIVSWIVVVLRGWRWRLMMRPLSKEITRLEAYDAYNICYLANLVLPRS
                     GEVVRCGMIANSGKATF"
     gene            435..1436
                     /locus_tag="PLI69_02460"
     CDS             435..1436
                     /locus_tag="PLI69_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOG41389.1"
                     /translation="MKSILGIGNALTDILALLPDNTLLTQFNLPEGSMQHVDAKTGNI
                     IWESLKQIGIQYVAGGSAANTISGAAIFGMPAGFIGKVGDDELGSLFKAEQVQNGIRS
                     NLLKGTAASGRAMVFITAPNADRTFATYLGAALQLEPEDLKPEYFDGYDYLYLEGYLV
                     QNQRLIRRAVEIGKEKGMIICLDMASYNVVESNNAFLHDIVKNYIDIVFANETEATSF
                     TGQEPRKALDIIATLCDIAIVKIGKDGSMLRQGDEYHYIEAWPANTIDATGAGDIYAA
                     GFLYAHALGMPLKICGEVGSIISAKVVEVIGPKIDIPRWKAAKIEIRDHIAPYLQNI"
     gene            1540..2163
                     /locus_tag="PLI69_02465"
     CDS             1540..2163
                     /locus_tag="PLI69_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG41390.1"
                     /translation="MNEFEKTDNEQLILKAAEQEFLTKGYDGARTISIAQKAGVSHAM
                     LHYYFRTKKQLFDRIMVKTIDAIQQTIVAAMGNPNMLFVERLKDGIGSLFDTMVANPL
                     LPRFFMNEIISRPELNSLLYERVASFVKKMVTELQKEADKAAERGEIEKTDIRELLIT
                     VMSLNIFPFLAYPFIEPVMGDMTIDRDKFFAERKAENIEIIMKRIKK"
     gene            2170..3453
                     /locus_tag="PLI69_02470"
     CDS             2170..3453
                     /locus_tag="PLI69_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG41391.1"
                     /translation="MRRLSLSFILALLTITLTAQTLDECRRLAREHYPEIRQYNLVAQ
                     TEQFNLSNAAKAWIPQFTISGQATYQSATPTLPEAFEALMKMQDADVEMTEINKDQYR
                     IALDVSQIIWDGGYSRANRAIAKAEAAEQRSLIDVSLYDLQTRVDGLYFGILLLDERV
                     EQTKAMIGVLESNLKRMLTYLKNGVALQSDVDMIEAELLTAQQQLSLVESSLASYRRM
                     LEIFIGQPLNSNKLERPVEQIIHDYISARPEFALLDAQSNRLAAQKIAVNSSVMPRLA
                     LFAQGYYGYPGMDIFKSMTSPDLSLNGIVGVRLSWDLSAFYTRNNNLKKIEVAQQQLA
                     VQRDVLQFNTKMQTTQEDGEIGRLRKVIENDARIVELRRSVRLAAESKLQNGVIDATD
                     LLRKITDETTAILNCNIHEIELLQAIYRLKHTLNE"
     gene            3459..4346
                     /locus_tag="PLI69_02475"
     CDS             3459..4346
                     /locus_tag="PLI69_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOG41392.1"
                     /translation="MKRIIYIAIVILSVSSCVTNGKYDAQGTFDATEVVLSSEATGRI
                     LSFDVEEGMPVRAGVVLGTIDSLQLILQQKQLSAQLSALLDSRPDKKAQVASLREQIA
                     KQKIELNRVKNMLKDGAATQKQYDDLEAQIRILKSQLSATLSTLDKSTATINNNAEAL
                     EAQIEALSDLISKCNIVSPIDGIILVKYAQAGELATMGRPLMKIADLDKIYLRAYFTS
                     GQLADIKLGDEVTVTADFGGTELYEYAGRIIWISSESEFTPKTIQTEDSRANLVYAVK
                     IAVENDGRLKIGLSGHVTL"
     gene            4348..5247
                     /locus_tag="PLI69_02480"
     CDS             4348..5247
                     /locus_tag="PLI69_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG41393.1"
                     /translation="MNVIEVNNLSKSYGKVKALESVSFSVERGELFGLIGPDGAGKTT
                     LFRLLTTLLNSDKGGATIEGFDISKDYRKIRPLLGYMPGRFSLYPDLTVEENLSFFAS
                     IFGVRVKDSYDLIAPIYQQIEPFRKRQASKLSGGMKQKLALSCALIHHPSVLFLDEPT
                     TGIDAVSRSELWDMLKNLKEKGITILVSTPYMDEASLCERIALFNEGRILGIDTPKGI
                     IKQFKSRLFAIRSDNMYSLLEAAREIEGVIECYPFGQMHHLIVDSKFDPHRFTEQLGS
                     LTNLTIEPAKPTIEDVFINLMKQ"
     gene            5250..5740
                     /locus_tag="PLI69_02485"
                     /pseudo
     CDS             5250..5740
                     /locus_tag="PLI69_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     assembly_gap    5741..5750
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5751..6094
                     /locus_tag="PLI69_02490"
     CDS             <5751..6094
                     /locus_tag="PLI69_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG41394.1"
                     /translation="LPLGWKQKLAFSVAVFHHPGIVFLDEPTGGVDPVTRRQFWEMIY
                     RAAERGTTIFVTTHYMDEAEYCSRVSIMVDGEMRALDTPARLKEEFNAESMHDLFRTL
                     ARDAERGELYP"
     gene            6091..7185
                     /locus_tag="PLI69_02495"
     CDS             6091..7185
                     /locus_tag="PLI69_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG41395.1"
                     /translation="MRNSFLSFVKKESLHILRDKVTMLIVMLIPVVLIVLFGFAISTE
                     VNNVNVAVVAPERTDGVKDAVDRIAHNDYFTFCGYIEPSQIDHELRSGKVSAVIVFSS
                     DFDRRLEQVSTGLPSTPIIQLVVDASNVNTAGASTAYLQEILLGTASLQAMFDTRMLF
                     NPQMKSAYNFVPGIMGLIVILVCAMMTSVSIVREKEVGTMEVLLVSPVRPTKIIFAKM
                     IPYFVISCVVLIIILLLSYYLLNVPMSGGVWGIFSVSLLYVLLSLSLGLLVSIVAKTQ
                     VMALLISAMVMMMPMMLLSGMLFPIENSPKFFQVLSNIIPARWYVEAIRKMMIQGSPF
                     LAVWKEFTILLGMTITFLGVSLIKFNDKLE"
BASE COUNT         2063 a         1543 c         1582 g         2007 t
ORIGIN      
        1 caaaggtagc cttcccgcta ttggcaatca tgccgcacct gaccacttca cccgagcgtg
       61 gaagcaccag atttgccaga taacagatat tgtaggcatc gtaagcctct aaacgagtaa
      121 tttctttact cagcggccgc atcataagac gccatctcca tccccttaaa accacaacaa
      181 tccaggatac tatcattgag agaagtatcc accagtagtt gcagctgcgc aatccggcca
      241 caaattcatc ccaacttata ttgcggaatg caaaatagag caagaaacct gcaagtgcaa
      301 gcatcatcag atactttata acatcttttg tcgttacctt cttagttctc ttcttcatct
      361 taatctcggt ttgtaggaca aaagtacaaa aattattatg tttgtggtca aacaaacaga
      421 aaacaccata tctgatgaaa tctattcttg gcataggcaa cgcgctcacg gacatacttg
      481 ctctccttcc cgacaacaca ctccttacac aattcaacct gcctgaaggc agcatgcaac
      541 atgttgatgc caaaaccggc aacataatct gggagagtct caaacagata ggaattcagt
      601 atgtagccgg aggttctgca gccaacacta tctcaggagc tgccatcttt ggtatgccgg
      661 cggggtttat aggcaaggtg ggcgatgatg agctgggttc tctcttcaag gcagaacagg
      721 tacaaaatgg tatccgctcc aatcttctca agggtaccgc agcttcaggt cgagctatgg
      781 ttttcatcac tgctcccaac gctgacagga cttttgcaac ctaccttggc gcagcccttc
      841 agctggaacc agaagatcta aaacctgaat atttcgatgg atatgactac ctctatcttg
      901 agggttatct ggtgcagaac cagcgtttaa tccgccgtgc agtagaaatc ggcaaggaga
      961 agggaatgat aatttgtctt gatatggcca gctataacgt agtagagtca aataacgctt
     1021 tcctccatga cattgtaaaa aactacatag acatagtttt tgcaaatgag accgaagcca
     1081 cttcattcac aggtcaggaa ccccgtaaag cacttgatat catcgcaact ctctgcgata
     1141 ttgctatcgt taagataggc aaagatggct caatgctacg ccagggcgac gagtaccact
     1201 atatcgaggc ctggcctgca aatacaatcg acgccacggg ggccggagat atatatgctg
     1261 caggatttct ctatgcccat gctttaggta tgccattaaa aatttgcggg gaggtaggct
     1321 ccattatctc cgcaaaagta gtcgaagtta tcgggccaaa gattgacatt ccacgctgga
     1381 aagctgcaaa aatcgaaatt agagatcata ttgcccccta tttacaaaat atttagagca
     1441 taattcggaa atattttatt gttctgaatt attcttttat ttttgcgtta acaaaattgt
     1501 taaacaaatt tgttaaatat tataattatc aggttattta tgaatgagtt tgaaaaaaca
     1561 gacaatgagc aactgatatt gaaggctgcc gaacaggaat ttttgacaaa gggatatgat
     1621 ggtgcccgca caatcagcat tgcccaaaag gcgggagttt cacatgctat gttgcattac
     1681 tatttccgaa ccaaaaaaca actctttgat cgcatcatgg tgaagactat agatgccatt
     1741 caacaaacga ttgttgctgc aatgggcaat cccaatatgc tatttgttga acggctcaaa
     1801 gatggcatag gctctctttt tgacactatg gtggccaatc ccctgctgcc gagatttttc
     1861 atgaatgaga ttatttcccg tccggaactc aacagtttgt tgtatgaacg ggtagcgagt
     1921 ttcgtaaaaa agatggtcac cgaacttcaa aaagaggctg ataaagcagc tgaacggggt
     1981 gaaatagaaa aaacagatat tcgggaacta ctgattacag taatgtcgct gaatattttc
     2041 ccattcttag cttacccatt tattgagccc gtcatgggtg atatgaccat agacagagat
     2101 aaattttttg cagaacgcaa agcggagaat attgaaataa taatgaagcg tataaaaaaa
     2161 taatggatta tgagacgact ttctttatct tttatactgg ctctcctaac cataactctc
     2221 actgctcaaa cacttgatga gtgtcgccga cttgctaggg agcattatcc cgaaatcagg
     2281 caatacaatc tcgttgcaca gacagaacag ttcaatttat caaatgctgc aaaagcctgg
     2341 ataccgcaat ttaccatatc cggacaggct acataccagt ctgctactcc aaccttaccc
     2401 gaagcctttg aggcattgat gaagatgcag gatgctgatg tagaaatgac ggaaataaac
     2461 aaagatcagt acaggattgc cctcgatgta agtcagatca tttgggatgg aggttattca
     2521 agagctaacc gggcgatagc taaagcagag gcagccgaac agcgtagtct tattgatgta
     2581 tcgctctatg atctacagac gagggttgat ggtctatact ttggtatctt actgcttgac
     2641 gagcgtgtag agcagaccaa ggccatgata ggagtgctgg agagtaattt gaaacgtatg
     2701 ctgacatatc ttaaaaacgg ggtagcattg cagtccgatg tagatatgat tgaagccgaa
     2761 ctgcttaccg ctcagcaaca gttgtctttg gtagaatcct ccctggcgag ctatcgccgt
     2821 atgctggaga ttttcattgg ccagccgctc aattccaata aattggaacg acccgttgag
     2881 caaattatac atgattatat ttcggcacgt cccgagtttg ccctgctgga tgcacagagt
     2941 aacagattag cagcacaaaa aatagccgta aactcatctg taatgccccg tttggctcta
     3001 tttgcgcagg gctactatgg ctacccgggg atggacattt ttaagagtat gacctctccc
     3061 gacctatcgc tcaatggtat tgtaggagtg cgtctgtcgt gggatcttag tgcattttat
     3121 acaagaaaca ataatcttaa aaaaatagag gttgctcaac aacaattggc agttcagcgt
     3181 gatgtattac agtttaatac taaaatgcag accactcagg aggatggcga gattggccgt
     3241 ctccgcaagg tgattgaaaa tgatgcccgt attgtagaat tacgccgttc cgtacgcttg
     3301 gcggcggaat caaagctgca aaacggggtt attgatgcta ctgatttact acgtaaaatt
     3361 actgacgaga ctactgcaat actcaattgc aacatacatg agatagaact attacaagct
     3421 atttacagat taaaacatac attaaacgaa taaaatatat gaaacgaata atctacatcg
     3481 ccatagtcat actttccgta tcatcatgcg ttacaaacgg aaaatacgat gcccagggca
     3541 cttttgatgc cacagaagtg gtactctctt cggaggctac gggacgcatt ttgagttttg
     3601 acgttgagga gggaatgccc gtccgtgcag gtgttgtttt aggaaccatc gatagtttgc
     3661 aacttatcct ccagcaaaag cagttatcgg cacaactttc agcgctattg gatagtcgtc
     3721 cggacaaaaa ggcacaggtt gcttcgttgc gtgagcagat agcaaaacaa aaaattgaac
     3781 ttaatcgcgt taaaaacatg ctaaaggatg gtgcagccac acaaaaacaa tatgatgacc
     3841 ttgaggcaca aatacgtatt ctaaaaagtc aactttccgc aacgctctcc actcttgaca
     3901 aaagcacggc aactatcaat aataatgcgg aagctctcga agctcaaatt gaagccctgt
     3961 ctgatctgat ttcaaaatgc aacatagtct cccccattga tggtatcata ctggtaaaat
     4021 atgcccaggc cggcgagttg gcaacaatgg gccgaccctt gatgaaaatt gcagaccttg
     4081 acaaaattta tcttcgggcc tatttcacat caggtcaact tgccgatata aaacttggag
     4141 atgaggtaac agttacagct gatttcggtg gtactgagct ctacgagtat gcaggtcgta
     4201 ttatttggat ctcttctgaa agtgaattta ctcccaaaac catacaaacc gaagattcac
     4261 gcgctaatct ggtctatgca gtcaaaattg ccgtagagaa tgacgggcgc cttaaaatcg
     4321 gcttgtcagg ccatgtaacc ctgtagtatg aacgtcatag aggttaataa tctctcaaag
     4381 agttacggaa aagttaaagc tcttgaatcg gtctcctttt cggttgagcg aggcgaactg
     4441 tttggtctca taggcccgga tggtgcgggc aagactacac ttttccgttt gcttactaca
     4501 ttgctgaatt ccgacaaagg aggtgccacg atagagggtt tcgatatcag taaggattac
     4561 cgcaaaattc gcccactttt aggttatatg cccggcagat tctcccttta ccctgatctt
     4621 actgtggaag agaatctcag tttctttgcc tctatcttcg gcgtcagggt taaagattca
     4681 tacgatctta ttgctccaat ctatcaacaa attgaacctt tccggaaacg ccaggcgagc
     4741 aaactgtcag gaggcatgaa acaaaaacta gccctctcgt gtgcattgat tcaccatccg
     4801 agtgtactct tcctcgatga gccgactacg ggtattgacg ctgtatcacg cagtgaactc
     4861 tgggatatgt tgaaaaatct gaaagagaaa ggaattacca tcctggtatc aaccccttat
     4921 atggacgagg cctcgctttg tgaacgaatt gctctcttta atgaaggacg cattttgggt
     4981 atagacaccc caaaaggaat tattaaacaa tttaaatcca gactctttgc cattcgttcc
     5041 gacaatatgt actccttgct ggaagctgcg cgagagatcg aaggcgtgat agaatgttat
     5101 ccatttgggc aaatgcatca ccttattgta gattcaaagt ttgacccgca tcgctttaca
     5161 gagcagttgg gatcattgac caatctcact attgaacccg ctaaacctac tattgaggat
     5221 gtatttataa atctgatgaa acaatgagcg aaatagtgat ttcagtacac aaccttacca
     5281 aaagttttgg taaattcatt gctgtaaaca atatctcttt tgatgtttat agaggtgaga
     5341 tttttggttt tctcggagca aatggcgcag ggaaaaccac ggcaatgcgt atgctaagcg
     5401 gattgagcta tcccacttcg ggaaagggca tggtggcagg ttttgatatt tcgcgtgaag
     5461 gagagatgat taagcgcaat atcggttata tgagccaacg atttgctctt tataacaccc
     5521 ttaccgttgg tgagaacatc aaccttttcg caggcatcta cggcctctct aaaaagagtg
     5581 ctgctaagcg tactgaggag ctgcttgcag aactcaaatt ttcttcggag cgtgacactc
     5641 tcgtaggttc attgccatta ggttggaagc aaaagttatc ttttgcaatt gccaccgttc
     5701 ataatcctgc cgttgtcttt ctggacgaac ctaccagtgg nnnnnnnnnn cgcttccgct
     5761 gggctggaaa caaaaattag ccttttcggt ggcagtcttc caccacccgg gaattgtatt
     5821 tttggatgaa cctaccggag gtgtcgatcc tgttacccgc cgtcaattct gggaaatgat
     5881 ttatagggcg gcggagagag gcactacgat atttgttact acccactata tggatgaggc
     5941 ggagtattgt tcccgtgtct ccattatggt ggatggcgag atgcgggcac tcgatacccc
     6001 tgcccgttta aaggaagagt ttaatgccga gtcaatgcat gatctgtttc gtacgttggc
     6061 tcgcgatgca gaaagaggag aattgtatcc atgagaaaca gctttctatc attcgtaaaa
     6121 aaagagtcac tgcatatttt gcgcgataag gttacaatgc tcattgtaat gcttattccg
     6181 gtagtattga tagtgttgtt cgggtttgcc atctctaccg aggttaataa tgtaaatgtg
     6241 gcagtagtgg ctcccgaacg tactgacgga gtaaaggatg ctgtagatcg catcgcacac
     6301 aatgactact tcaccttctg cggttatatt gaacctagtc agattgatca tgaattgcgt
     6361 tcgggaaaag tgagtgcagt catagttttt tcctcagatt ttgaccgtcg cttagagcag
     6421 gtttcaacag gattgccctc aactccgatt atacaattag tggttgatgc ctctaatgtc
     6481 aatacggccg gggcctcaac tgcttatttg caggagatcc tactcggcac agcatcactg
     6541 caagcaatgt ttgatacgag aatgcttttc aatccccaaa tgaagagtgc ctacaacttc
     6601 gtgccgggca ttatgggttt gattgtcatt ctggtttgcg caatgatgac ttcggtatcg
     6661 atagttcgcg aaaaagaggt tggaacaatg gaagtgcttc tcgtttcgcc cgtacgtcct
     6721 acaaagatca tttttgctaa aatgatacct tattttgtta tctcctgtgt ggttttgata
     6781 ataatactgc tcctttctta ttatctgctt aatgtaccta tgtctggtgg cgtttggggt
     6841 atattttcag tatcactgct ctatgtatta ttatcgctct ctctcggact tcttgtctcg
     6901 atagttgcca agacacaggt aatggcactg cttatttcgg ctatggtaat gatgatgccc
     6961 atgatgttgc tttcaggcat gctcttcccc attgagaatt ctcccaagtt ttttcaagta
     7021 ctctcaaata ttatccccgc acgttggtat gtggaggcca tccgcaaaat gatgattcag
     7081 gggtctcctt ttctggccgt atggaaagag tttaccattt tgctcggaat gacaatcact
     7141 ttcttaggcg tatcgctaat caagttcaac gataaattag agtagtgcca tgagaatctt
     7201 tatcg
//
LOCUS       DAOSIE010000048         7184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9482_length_7184_cov_3.684107, whole genome shotgun sequence.
ACCESSION   DAOSIE010000048 DAOSIE010000000
VERSION     DAOSIE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9482_length_7184_cov_3.684107"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1448
                     /locus_tag="PLI69_02500"
     CDS             90..1448
                     /locus_tag="PLI69_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOG41396.1"
                     /translation="MSEQIRLKKGLDLPVKGAALRKISMTVVPDVVALKPTNFRSLTP
                     KMLVKEGDTVKAGTPIFADKKNPEILFTSPVSGTVDTIVRGEKRKLLEVRIKSDNLGA
                     AVKFNVPTGASLNRESIIKHLLESGLWPCIKQRPYGIIADPSVTPKSIFISAMATGPL
                     AADPEYVLGGELDAIRKGLAVLGKLTEGGVHLSLDADKAASSIFNKFDGVVYHYFKGP
                     HPAGNVGVQINHISPISKGETIWTVDLFLVAAIGKLFLHGVYDTSRIIAVAGPAVVNP
                     SYVKCCAGVSMEQISDLFNFAHGNIRIISGDLLSGKAVGREGFLGFYDNLVTALPEGD
                     YYEMFGWVKPFRLKKFSSSRTYFSWLMPRKKYNMDTNTNGGERAFVLSDVYDKVLPMD
                     IYPLYLFKAILADDIEKMENLGIYEIIEEDVALCEFVCPSKIEIQSIVSRGIELMINE
                     MS"
     gene            1463..2605
                     /locus_tag="PLI69_02505"
     CDS             1463..2605
                     /locus_tag="PLI69_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOG41397.1"
                     /translation="MSKLRKLTDKIKPTFEKGGKLSFLYSTFEAFETFLFVPNTVTVK
                     GVHVRDSVDLKRVMTTVMIAIVPSLLFGIFNIGYQHNLAANAGMEFWEMVWYGFLKII
                     PLYLVSYIVGLGIEFASAQIRGHEVNEGYLVTGLLIPLIVPVTTPLWMLALAVAFAVI
                     IGKEIFGGTGMNIWNPALLARAFLFFAYPSHMSGDKVWVAGVDGFSGATPLAQTSEGL
                     SSLQYSVSDMFFGFIPGSVGETSTLAILLGAVILIYTGIGSWKIMISTVGGALVLGLI
                     ANLAAPSPDSAMAIPAWYHLIMGGFAFGTVFMATDPVTASQTEQGKWVYGFLIGALTV
                     TIRLFNPGYPEGMMLAILLMNTFAPLIDHIVVGRNIKRRLKRAKTV"
     gene            2609..3481
                     /gene="nqrC"
                     /locus_tag="PLI69_02510"
     CDS             2609..3481
                     /gene="nqrC"
                     /locus_tag="PLI69_02510"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOG41398.1"
                     /translation="MKLNTNNNIYTILYTTILVVIVAGVLAYASTALKPKQQENIALE
                     KMQSLLRTARLGEDASAQDNKSEYIKALYSKHMTNDFVLNYEGKVIEGEAFETDLQAQ
                     YNIIKQIVAAKTPEAAQALRERLRLPVFVCTIDDKVLNIFPCYGPGLWGPIWGYIAVE
                     DDFETIYGAVFDHKGETPGLGAEIAHATFYNQFVGKKLSKDGVFKSISIVKGGAQADN
                     PNGVDAISGGTITSQSLENAIAIWLNEYQAYFNIKRAAIGSDECCDEEPCTGDDCDSH
                     DETRNQEGHEDHTK"
     gene            3490..4110
                     /locus_tag="PLI69_02515"
     CDS             3490..4110
                     /locus_tag="PLI69_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOG41399.1"
                     /translation="MDKKIFKNPFFKANPITVLVLGICSALAVTVQLKGAIVMALSVT
                     VVTGFSSMLVSSIRNSIPNRVRIIVQLVIVAMLVILVDELLKAYAYDVSKQLSVYIGL
                     IITNCIIMGRIEAFALGNKPFPSLVDGLANGMGYGLVLVIIAFIRELFGSGTLFGVQI
                     IPQNWYMANGGFYENNGMIILPPMALIIVGAIIWVQRSVQKELQEE"
     gene            4128..4742
                     /gene="nqrE"
                     /locus_tag="PLI69_02520"
     CDS             4128..4742
                     /gene="nqrE"
                     /locus_tag="PLI69_02520"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOG41400.1"
                     /translation="MEYINIFVKSVFVENMVFAFFLGMCSYLAVSKSVKTATGLGLAV
                     IFVQVVTLPINYLLNKYLLEPGALVWLNPEYVSVDLSFLSYIVFIAVIASIVQIVEMV
                     VEKFSPALYSSLGIFLPLIAVNCAILGGSLFMQERGYADIGEATSFAFGSGFGWFIAI
                     VAIAAIREKLAYSNVPKPLKGLGISFIITGLMGIAFMSFMGIKL"
     gene            4749..6011
                     /gene="nqrF"
                     /locus_tag="PLI69_02525"
     CDS             4749..6011
                     /gene="nqrF"
                     /locus_tag="PLI69_02525"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOG41401.1"
                     /translation="MDVNVLIITIVASLALILPLVALLLYIKAKLTPSGNVIININNG
                     SKEIAVSQGSSLLATLANEKIFLASACGGKGTCGECKCQVLEGGGTILPTEVGFFTRK
                     QILDNWRLGCQVKVKDNLKILVPESTLSVKKWECEVVSNDNVATFIKEFCVKLPEGED
                     LNFRSGGYIQVDVPPMRLNYSDFEIDEKYREDWKKMGMFDLKMVNSEETLRAYSMATY
                     PAEGNIIKLNVRIATPPINRETGKFENVNPGVCSSYIFSLKSGDKVMISGPYGEFFVP
                     DDVPAEQEFLFVGGGAGMAPLRSHIMHLFKTEKTTRKVNFFYGARSLKEAFYLEDFKA
                     IEKEFPNFKFHLALDRPDPVADAAGVPYVPGFVHNVMNETYLKDHEAPEDILYFMCGP
                     PMMVQSVNTLLDSLGVEPESILYDDFGG"
     gene            6127..6199
                     /locus_tag="PLI69_02530"
     tRNA            6127..6199
                     /locus_tag="PLI69_02530"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6161..6163,aa:Glu,seq:ctc)
     gene            6270..>7184
                     /locus_tag="PLI69_02535"
     CDS             6270..>7184
                     /locus_tag="PLI69_02535"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOG41402.1"
                     /translation="MEQQYFASRNHAIDLLRAMIMVLMVFVNDLWRIASYPRWLGHAA
                     VGEDFLGLSDIVFPCFLFVVGMSIPYALEGRYAKGKGTRSTLRHILTRSLALMVMGVF
                     LVNTESGISPDAFMPNTVYKLFLLVAFFLIWNYKSFKVLQIAGVVLLLFLAFIFKDPS
                     GGYLSARWWGILGLIGWSYLFCAITYIFTRARLKYIVAVWGALLLVNILLTPIREGVS
                     VSESGLLFGLPKINILGDFADALHVGNGHTQLLTMGGVMLSVISMKMDSMKVSTKTLT
                     AAGAVIVLLLATWLARQLWIMDKIGGTLP"
BASE COUNT         1811 a         1548 c         1729 g         2096 t
ORIGIN      
        1 ttcaaaggat ataatattga tttttataca tatctttgca gccatacacg taattactta
       61 agtgttaaat caataatcta aactaaataa tgtcagaaca aattagattg aagaaggggt
      121 tggatttgcc tgttaaaggg gcagcccttc gcaaaatatc catgacagtt gttcctgatg
      181 ttgtcgccct caaacctacc aatttccgta gtctgactcc gaaaatgttg gtaaaggagg
      241 gtgataccgt taaagcaggt acacccatct ttgctgacaa gaaaaatccg gagatactat
      301 ttacatctcc ggtaagtggc acagttgata caattgtacg aggagaaaaa cgcaaattgc
      361 tggaggtaag gatcaaatcc gacaacctgg gagcggctgt gaagtttaac gtaccgacag
      421 gagcttccct aaacagagag agcatcatca agcatctgct tgagagcggt ctatggccct
      481 gcatcaaaca gagaccttac ggcatcatcg ccgatccctc cgttactccc aaatccatat
      541 tcatatctgc aatggccaca ggtccacttg cagctgatcc ggagtatgtg ctcgggggag
      601 aattagatgc catccggaag ggtcttgcgg tgttgggcaa gcttactgaa ggaggtgttc
      661 atctctctct cgatgcagac aaggctgctt caagcatttt caacaaattt gacggagtcg
      721 tctatcacta ctttaagggg cctcaccccg cgggcaatgt tggagtacag attaatcaca
      781 tcagcccaat ttccaaaggt gagactatct ggacggttga tctgttttta gttgcagcca
      841 tcggtaaatt gttcttacac ggcgtgtatg atacaagtcg catcattgca gttgccggtc
      901 cggcagttgt gaatccttct tatgtgaagt gttgtgcagg agtttcgatg gagcagatct
      961 cagacctttt caatttcgca catggcaata tccggattat cagcggtgat ttgctttccg
     1021 gcaaagccgt aggcagagaa ggtttcctcg gtttctacga caatcttgtg acagctctcc
     1081 ctgagggaga ctactacgag atgttcggat gggtaaaacc tttccggctg aagaaattca
     1141 gctcttcacg tacttatttt tcgtggctga tgccacgcaa gaaatataat atggacacca
     1201 ataccaatgg cggagagagg gcttttgtac tctctgacgt ttatgataag gtgcttccca
     1261 tggacatata tcctctctat ctattcaagg ctattttagc cgatgatata gagaagatgg
     1321 aaaacctcgg tatatatgag attatagaag aggacgtggc tctctgcgag tttgtttgtc
     1381 cttctaaaat cgagattcag tccatagttt ccagaggtat tgaacttatg attaatgaga
     1441 tgtcataaat aggaggtaaa gcatgagcaa acttagaaaa ttgacggaca agatcaagcc
     1501 aaccttcgaa aagggtggca agctctcatt cctatactcg actttcgagg cttttgagac
     1561 ttttctattc gtacccaata ccgtaacggt aaaaggggtg catgtcaggg atagcgtaga
     1621 cctcaaacga gttatgacta cggtcatgat tgcaatcgta ccctctctgc tcttcggtat
     1681 ctttaatatc ggataccagc acaatctggc agccaatgca ggtatggagt tttgggagat
     1741 ggtttggtac ggattcctca agattatccc tctctatctg gtttcctaca tagtgggtct
     1801 gggaatcgaa tttgcctcgg cgcagatcag aggccatgaa gttaatgagg gttacctagt
     1861 taccggactc cttattccgc ttatcgttcc cgtcactact cctttgtgga tgcttgcact
     1921 tgcggtggca ttcgcagtaa ttataggtaa ggagattttt ggcggtacag gtatgaatat
     1981 atggaatccc gctctgctgg ccagggcttt tctcttcttt gcctatccca gccacatgtc
     2041 gggtgacaag gtatgggttg caggagtaga tgggttttcc ggcgcaactc ctctggctca
     2101 gacttcagag ggactctcct ctctacaata ttcggtaagc gatatgttct tcggttttat
     2161 accgggttca gtgggcgaga cttctactct ggctatcctt ctgggagccg taatactaat
     2221 ctacacgggg ataggtagtt ggaagattat gatctcaaca gttggcggag ccctcgtatt
     2281 gggtttgatt gcaaatctcg ctgctccatc ccccgattct gcaatggcaa ttccggcctg
     2341 gtaccatctt attatgggag gtttcgcgtt cggtaccgtg tttatggcaa ctgacccggt
     2401 tacagcatcg caaacagagc aaggcaagtg ggtctatggt ttccttatcg gagcacttac
     2461 agtgaccatc agactcttca acccgggata ccccgaggga atgatgctcg caatacttct
     2521 gatgaatacc ttcgcaccgc taatagacca tattgtggtc ggcagaaata ttaaacgcag
     2581 attaaaaagg gcaaaaacag tatagattat gaaattgaat accaacaaca atatctacac
     2641 gatcctctat accactattc tggtggtgat agtagcaggt gttcttgcct atgcatcgac
     2701 tgcacttaag ccgaaacagc aagaaaacat cgcacttgag aaaatgcagt ctctgctgcg
     2761 tacggcaaga cttggtgagg atgcatctgc tcaggacaac aagagcgaat atattaaagc
     2821 tctctattca aagcatatga ccaacgattt tgtactcaac tatgagggta aggtgattga
     2881 gggagaggcc tttgaaaccg accttcaggc tcagtacaat ataatcaaac agattgttgc
     2941 agccaagact cctgaagctg ctcaagctct tcgagagcgg ctccgacttc ctgtatttgt
     3001 ctgcacgatc gatgacaaag tcctcaatat tttcccctgc tacggcccag gtctttgggg
     3061 accgatctgg ggatatattg cagttgagga tgattttgag accatctacg gagcagtatt
     3121 tgaccataag ggcgagacac ctggcctggg cgcagagata gcgcatgcca ctttctataa
     3181 ccagtttgtg ggtaaaaaac tctctaagga tggtgttttt aagtctatct ccattgttaa
     3241 aggtggcgcg caggcagata atcccaacgg agttgatgcc atctcaggag gaacaattac
     3301 cagtcagtct cttgaaaatg ccatcgcaat ttggcttaat gagtatcaag cctatttcaa
     3361 tattaaacgg gctgcaatcg gttctgatga gtgttgtgat gaagagccgt gtactggtga
     3421 cgattgtgac agccacgatg aaacccggaa tcaggaaggc catgaagatc atactaaata
     3481 agagttatta tggataagaa aatatttaaa aacccatttt tcaaggccaa tccgatcacc
     3541 gtactggtgc tcggcatctg ttccgctctt gcagttacgg ttcagttgaa gggggccata
     3601 gtaatggcgc tttcggtgac ggtagttacg ggcttctcca gcatgctggt ctcctcaata
     3661 cgcaattcca tccctaatcg cgtcaggatc attgtgcagc ttgtaattgt ggctatgctt
     3721 gttatcctgg ttgacgagtt gttaaaggct tatgcctacg atgtaagcaa acagctttct
     3781 gtttatattg gcctgattat taccaactgt atcataatgg gccgtattga ggcctttgcg
     3841 cttggcaaca agccattccc ttcccttgtc gatggccttg ccaatggcat gggctatggt
     3901 ttggtcctcg taatcatcgc atttatacgt gaacttttcg gctcaggtac tctgtttggt
     3961 gtgcagatta ttccccaaaa ttggtatatg gccaatggcg gtttctacga aaacaacggt
     4021 atgataatac tccctccgat ggctttaatt atcgtgggtg ccattatctg ggtacagaga
     4081 tccgttcaga aagaattaca ggaagaataa attgaggagg attatttatg gaatacatca
     4141 atatatttgt aaagtcggtt ttcgtagaaa acatggtgtt cgcattcttc cttggaatgt
     4201 gttcttacct tgccgtatcc aagagtgtca agacagccac gggacttgga ctggctgtaa
     4261 tatttgtaca agtggttact ctcccgataa actatttgtt gaacaaatat ctgcttgaac
     4321 ccggtgctct tgtctggctc aatcctgagt atgtatcagt tgatctaagc ttccttagct
     4381 atatagtatt tattgcggtt atcgcttcga ttgttcagat cgtggagatg gtcgttgaaa
     4441 aattcagtcc ggctctctac tcatcactgg gtattttcct ccccctgatt gccgttaact
     4501 gtgcaatcct cggaggttcg cttttcatgc aggagagagg ttatgcagat attggagagg
     4561 caacatcatt tgcttttggt tcgggatttg gctggttcat cgctatcgtg gcgatagccg
     4621 ccataaggga gaaacttgct tactccaacg ttcccaagcc attaaagggt cttggaatct
     4681 catttatcat taccggtctc atgggtatag cctttatgag ttttatgggt atcaaattgt
     4741 aggagaatat ggatgttaat gttttaataa tcactattgt tgcctctctg gcacttatat
     4801 tgccgcttgt agctttgttg ctatatataa aggcaaagct taccccttca ggcaacgtca
     4861 ttatcaatat taacaacggc agtaaagaaa ttgccgtttc tcaggggagt tctctactcg
     4921 ccactcttgc caacgagaag atttttctgg cttcagcctg tggaggcaag ggtacttgcg
     4981 gtgagtgcaa gtgccaggta cttgaaggag gcggaaccat tctacctact gaggtgggct
     5041 tctttacccg caagcagata ttggacaact ggcgtctcgg ctgccaggtt aaggtaaagg
     5101 ataatctgaa aattttagtg ccggagtcta ctcttagcgt taagaagtgg gagtgtgagg
     5161 ttgtttccaa tgacaatgtg gctaccttta taaaagaatt ctgtgtaaaa cttcccgaag
     5221 gagaggatct caacttccgt tcgggtggtt acatccaggt ggatgtgcct ccaatgagac
     5281 ttaactacag cgattttgag atagatgaaa agtaccgcga ggattggaaa aaaatgggaa
     5341 tgtttgacct taagatggtt aattccgaag agactctcag ggcctattca atggccactt
     5401 accctgctga gggtaacata attaagctca atgtgcgtat agccactcct cccattaatc
     5461 gcgagaccgg taaattcgag aatgtcaatc cgggtgtctg ctcctcatat attttctcgc
     5521 tcaagtcagg cgataaagtg atgatttcag gtccttatgg agaatttttc gtccccgatg
     5581 atgttcctgc tgagcaggag ttcttattcg taggaggtgg tgccggcatg gctcccttgc
     5641 gcagccatat catgcatttg ttcaagaccg agaagactac ccgtaaggtg aactttttct
     5701 atggagcccg tagtcttaag gaggcattct atcttgagga cttcaaggcg atcgaaaagg
     5761 agttccccaa cttcaagttc catcttgccc tcgaccgccc cgaccctgtg gctgatgccg
     5821 cgggagttcc ctatgttccg ggctttgtcc acaatgtgat gaacgaaacc tacctaaagg
     5881 atcatgaagc ccctgaagac atcctctact ttatgtgcgg ccctcctatg atggtacagt
     5941 ctgtaaatac tcttcttgac agtttgggag ttgaacctga gtctattcta tatgacgact
     6001 ttggcggttg attgccgaat ttagagagaa agtttgttat acgggcgacc tggaggttgc
     6061 ccgttttttg tcaaaagaga gaaaaaagtt ggatttttta aaaatatacc tatctttgca
     6121 gcccacggcc ctatcgacta gttggttagg tcgcaactct ctcaaggttg aaacacgagt
     6181 tcgaatctcg ttggggctac aataaggaat ggcataacag tcattccttt tttttgtatc
     6241 tttgactcca tatttttgat tctcatatta tggagcaaca atattttgca agtcgcaatc
     6301 atgccataga ccttctaagg gcgatgatta tggtgcttat ggtctttgtc aacgacctgt
     6361 ggcgcattgc ctcttacccg cgttggctgg gacatgctgc tgtaggagag gattttctcg
     6421 gtctttccga cattgttttc ccctgtttcc tttttgtggt aggaatgtcc attccttatg
     6481 cacttgaggg gcggtatgca aagggtaagg gcactagaag tactcttagg cacatactta
     6541 ccaggtctct ggcattgatg gtaatgggag ttttccttgt gaataccgag agcggaatct
     6601 ctcctgacgc ctttatgccc aatacagttt acaaactctt cttgcttgtg gccttcttcc
     6661 ttatatggaa ctacaagtct tttaaggtgc ttcagattgc cggagtggta ttgctgcttt
     6721 tccttgcttt tatcttcaaa gatccctcgg gaggttacct gagtgcgcgt tggtggggca
     6781 tcctggggct gatagggtgg agctatctct tctgtgccat aacctacatt ttcaccaggg
     6841 cgcgtcttaa atatatagtg gctgtctggg gtgctttact gctggtcaat atacttctca
     6901 ctccaattcg cgagggggta tccgtttcag agagcgggct gctgttcggg ctgccgaaga
     6961 taaatatact tggcgacttc gctgacgcac tacatgtagg taacggtcac acgcaactct
     7021 tgacaatggg cggagtgatg ctctcggtca tctcgatgaa gatggatagc atgaaagtct
     7081 ccactaagac tctgactgcg gcaggagctg ttatagtgct gttgctggca acctggctag
     7141 cccgtcagtt gtggattatg gataagatcg gtggcaccct ccct
//
LOCUS       DAOSIE010000049         7115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9594_length_7115_cov_3.762890, whole genome shotgun sequence.
ACCESSION   DAOSIE010000049 DAOSIE010000000
VERSION     DAOSIE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9594_length_7115_cov_3.762890"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PLI69_02540"
     CDS             <1..680
                     /locus_tag="PLI69_02540"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOG41403.1"
                     /translation="EVKDVHTTDLTSGRETLEEAQMVVFVGGFSNADTLGSAKGWAGT
                     LMYNKRAKETIERFFAREDTLSLGVCNGCQLMGELGYISLSDRASAPKLRHNASGKFE
                     SGFIGVTIPESPAIMLKSLQGCKLGVWIAHGEGKFEFPKPISEYNIALKYSYDAYPGN
                     PNGSPEAIAGVCSEDGRHLAMMPHPERCVRPWNWAYYPHGHRNDVVTPWMEMFVNARN
                     WCMAVSR"
     gene            complement(750..823)
                     /locus_tag="PLI69_02545"
     tRNA            complement(750..823)
                     /locus_tag="PLI69_02545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(787..789),aa:Arg,seq:acg)
     gene            complement(838..1212)
                     /gene="rplS"
                     /locus_tag="PLI69_02550"
     CDS             complement(838..1212)
                     /gene="rplS"
                     /locus_tag="PLI69_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOG41404.1"
                     /translation="MDLIKIAQEACAQELKQLPDFKAGDTVTVTYKIKEENKERFQKF
                     RGIVIQKRGEGATKTFTVRKISNGVGVERIFPLSSPLIEDIEVNKVGAVRRARIFYLR
                     KLTGKKARIKEKRVARQTPKAE"
     gene            complement(1352..2251)
                     /locus_tag="PLI69_02555"
     CDS             complement(1352..2251)
                     /locus_tag="PLI69_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOG41405.1"
                     /translation="MSKIVRYFLVSLKGLLMGAADVIPGVSGGTIAFLTGIYGELIDS
                     IKSIDLEAIKLLFTGKFREFWLKINGNFLLALVLGILISIFSLAKVMQYLLVHHPIPL
                     WSFFFGLILASPIYIIKEIESKKLIHLIPTIVGIAVGVFICLISPTETTEALWFVFLC
                     GAIGICAMILPGISGSFVLLLLGKYAFIIGAVSDLNIPVLLVFAAGAVIGIILFSNFL
                     SWLLKKAYNGTLFFLSGLMIGSLVKVWPWKKLLESGVDRPVLPGEYSGDPQLIQALIW
                     FAVGVLLLFGIEFLAKRLKSAKQ"
     gene            complement(2254..2748)
                     /locus_tag="PLI69_02560"
     CDS             complement(2254..2748)
                     /locus_tag="PLI69_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41406.1"
                     /translation="MRQPVLKPFNDARSIGVILNLRNSNCPDTVAFLDQEAARHGIKL
                     HYLLVNLHKHPIPQWCLDPRMTVINYKRDFTHTGKLKSDTADAFINTEFDFLFSVSTH
                     FHPVVKKVATLSKAGFKVGKYFSRDNPYDLTYRPSYSERDLELTKEYVANIHLITLSD
                     KSDD"
     gene            complement(2817..5534)
                     /gene="ppdK"
                     /locus_tag="PLI69_02565"
     CDS             complement(2817..5534)
                     /gene="ppdK"
                     /locus_tag="PLI69_02565"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOG41407.1"
                     /translation="MTKRVYRFGGKSAEGNGTMKNLLGGKGANLAEMCTLGIPVPAGF
                     TITTDTCTEYFALGGKYPEGLKEEVTEAIKATEDVMGMKFGDSKNPLLLSCRSGARMS
                     MPGMMETVLNIGLCSATIPGLIEKTKNPRFVYDAYRRLIMMYSDVVMEKSEGIEPEDG
                     KGIRVQLDNMMHDLKEKKGYKSDTDLTEEDLKDLCDRFKVRVKEVLGKEFPDDAQAQL
                     WGSIGAVFKSWNGKRAIAYRRIEGIPDEYGTAVNVQSMVFGNMGSNSATGVAFSRNPA
                     TGEHTFYGEWLVDAQGEDVVAGIRTPNPLNESTKNEHNKHLASLEKAMPEVYKELDHI
                     QDHLEHHFSDMQDIEFTIQDGKLWLLQCRVGKRTGFAALNMAMDMIEEGMIDEKTAVM
                     RVAPKQLDEILHPILDPEAEKKATVIAKGLPAGPGGAVGKLVFTSEAAIEAAAKGEKV
                     ILVREETNPEDVEGMRAAVGLLTSRGGMTSHAALVARGWGKCCIVGCDAIKFTTSGNK
                     RVMTIGNKTYKEGDVFSLNGTKGHVYDVAVSTVDASENPRFVEFMTMVDKFRTLGVRT
                     NADTPEDALKAIEFGAEGIGLFRIEHMFYGKDSEAPLSKLRKMIHASSLEERIDALKE
                     LEPFVVDATRETMRVMNGRPVTFRLLDPPLHEFVPQTLEKQKELAHELGITIDEIKER
                     GELLHEVNPMMGHRGVRLGITYPEVSHMQFKAIFTATAQLLKEGLDPQPEIMIPVTIS
                     PKELDFQKAICDKALAEVEAAEGVKIKYLYGTMIEIPRAVILAPEMAKSAQFFSFGTN
                     DLTQMTYGFSRDDIGAFMNDYMEHGILSFDPFQTLDLSSVGKLVKLGLDGGRSTSPKL
                     KVGICGEHGGDPDSVRFCYQIGMDYVSCSPFRLPIARLAAAQAAIADKK"
     gene            5757..6419
                     /locus_tag="PLI69_02570"
     CDS             5757..6419
                     /locus_tag="PLI69_02570"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOG41408.1"
                     /translation="MPVLRVKKVVFDSAKPSVAEVAAMFEREKIEFNPIAVVNWKEYG
                     YKPDVKFRIAHSAEEIYLQYVVKEEGVRATFGYDAGSKPYTDSCVEFFMIPSDKDSIY
                     YNLEMNCIGHGTFAGGPDRKQRTRFGDEVISQIRRESTLGSEPFGERAGEQEWTLTIA
                     IPKSIYSLSEFSDFSGRTVRGNFYKCGDEMPVPHYLSWHPIPIPSPNFHKPEHFGPIF
                     FE"
     gene            6475..6945
                     /locus_tag="PLI69_02575"
     CDS             6475..6945
                     /locus_tag="PLI69_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41409.1"
                     /translation="MKKLTLFSLVAAALISLSGCQFIRDLINEEYLLYGQWDVAKVYE
                     GEIELNYAETRYSFVFEKNGTFKFQIWESAAKTNMVIASYTGSFSYDKDKYILNISFP
                     IDGLLQGKKDFNTNIVSQFQVKELTLKRLLLDVIEEDELAIFNLNSTWEMNKKF"
BASE COUNT         1959 a         1629 c         1568 g         1959 t
ORIGIN      
        1 tcgaagtaaa agatgttcac actaccgact taaccagcgg acgagagact ttggaagagg
       61 ctcagatggt ggtctttgta ggaggatttt ccaacgccga cacactcgga tcggctaaag
      121 gctgggcagg tacgttgatg tataacaaaa gggcaaagga gaccatcgag cgtttctttg
      181 ctcgtgaaga tacactaagc cttggcgttt gtaatgggtg tcaattgatg ggagagcttg
      241 gttatattag cctttcagat agggcttccg cacctaagct gcgtcacaat gcctcaggca
      301 aatttgagag cggatttata ggagttacca tccccgaatc acctgcaatt atgctaaagt
      361 cccttcaggg gtgtaaactg ggagtctgga tagcacacgg tgagggtaaa tttgagtttc
      421 ctaagcctat ttctgagtat aatatagctc tcaagtatag ctacgatgca tatcccggca
      481 atcctaacgg ctcacctgaa gctattgcag gagtatgcag cgaagatgga cgacaccttg
      541 ccatgatgcc ccatccggag cgctgcgttc gcccgtggaa ctgggcctat tacccgcacg
      601 gacatcgtaa cgatgtggta actccgtgga tggaaatgtt tgtgaatgca aggaattggt
      661 gtatggccgt tagccgttag ccgttaaccg aattcagagc gaagcgactg tcacaaaaga
      721 gaggaacgat taatcgttcc tctcttttgt gaccccaaca ggattcaaac ctgtaacctt
      781 ttgatccgta gtcaaatact ctattcagtt gagctatggg gccttatgaa aaaactgtta
      841 ttcagctttt ggggtctgac gggcaactct cttctctttg atgcgtgcct tcttgccggt
      901 gagctttctt aggtagaata tgcgtgctct acgtactgca cctactttgt tgacctcgat
      961 atcctcaatc aggggtgatg atagagggaa aatcctctct acacctactc cattggagat
     1021 cttgcgtact gtaaaagtct ttgtagcgcc ctctccgcgt ttctggatta caataccacg
     1081 aaacttctgg aaacgctctt tgttttcttc cttaatctta taagtaacgg taactgtgtc
     1141 tcctgccttg aaatcaggaa gctgttttaa ttcctgtgca caggcttcct gtgcgatctt
     1201 aattaaatcc attttaaatt aatgttttag cgcaacattg ctgcttcgga agcctccgag
     1261 actaatgcca agaggttgct tttcgttttg ggactgcaaa agtacatatt ttttcagaag
     1321 gtacaaattt ttaggcactt tttcctcaat attattgttt tgcagatttt aatcttttag
     1381 cgagaaattc tattccgaac agtaggagca ctcccaccgc aaaccatatt agagcctgaa
     1441 tcaattgggg atccccacta tattctcccg ggagtaccgg acgatccact ccactctcga
     1501 ggagcttttt ccagggccag actttaacaa gagaacctat catcaggcct gaaaggaaaa
     1561 acaaggtacc gttgtaggcc ttcttaagca accaggagag gaaattagag aaaaggatta
     1621 taccgataac tgcaccggct gcaaaaacaa gaagaacggg aatattcagg tcgctaactg
     1681 caccaattat aaatgcatac tttccaagaa gaagcagaac aaaactgccg gagatccccg
     1741 gaagaatcat ggcgcaaatt cctatggccc cgcaaaggaa tacaaaccac agtgcttctg
     1801 tagtctcggt gggtgatatg agacagataa atactcctac tgcaattccg acaatggtcg
     1861 gaatcagatg aatgagtttc ttactctcaa tctctttgat tatgtagata ggtgaagcca
     1921 ggataagccc gaaaaagaag gaccacaaag gtatgggatg gtgtaccagc agatattgca
     1981 tcacctttgc taaagaaaag atgcttatca ggatacccag tacaagtgca aggagaaaat
     2041 tgccgttgat tttgagccaa aattcacgga atttccctgt aaaaaggagt tttattgcct
     2101 caaggtcaat agatttgatg gaatctatca gttcgccgta gatgccggta agaaaagcta
     2161 tcgtcccgcc tgatactccc ggaattacat cggcagctcc cattaaaaga cccttgaggg
     2221 agacaagaaa atagcgtact atttttgaca taattagtcg tcagatttgt ccgataatgt
     2281 tatcaaatga atgtttgcca catattcctt ggtaagttcc agatcccttt ccgaatatga
     2341 tggtctgtat gtaagatcat agggattgtc cctcgagaaa tatttgccca ctttgaaccc
     2401 tgctttggag agagttgcca cttttttcac caccggatgg aaatgggttg aaacagagaa
     2461 caaaaaatca aattccgtgt tgatgaatgc atccgcagta tcggacttga gtttgcccgt
     2521 atgagtgaag tcacgtttgt aattgattac agtcatcctg ggatcaagac accattgcgg
     2581 tataggatgc ttatgaaggt ttaccaaaag ataatggagt ttgatgccgt gtcgggcagc
     2641 ttcctgatct agaaaagcga ctgtatcggg acaattggag tttctgagat ttagtattac
     2701 accaatcgaa cgggcatcat tgaagggttt cagtacaggt tgtctcatat ttgcaccaat
     2761 aaaacaaaaa agactcccga acacaactat ggcgttcggg agtttttaga aacaaattac
     2821 ttcttgtcag caatggctgc ctgagcagct gcgagtcttg caatgggtag acggaaaggt
     2881 gaacatgata catagtccat ccctatctgg tagcagaaac gtaccgaatc agggtcgcca
     2941 ccatgctctc cgcaaatacc aaccttaagt ttcggactgg tggagcgtcc tccatcaagg
     3001 cctaacttta caagttttcc gacagaactc aaatcaagag tctggaaggg gtcaaaggag
     3061 agtattccat gctccatata gtcgttcata aaggcaccga tgtcatccct tgagaaacca
     3121 taggtcatct gtgtaaggtc atttgtacca aatgagaaga actgagcaga cttggccatc
     3181 tcaggtgcaa ggattactgc gcgggggatt tcgatcatag ttccatagag atatttgatc
     3241 ttaacaccct cagctgcctc gacctccgcc aaagctttat cgcaaatggc cttctggaaa
     3301 tcaagctcct tgggggagat ggttacagga atcataattt caggttgcgg gtcaagaccc
     3361 tccttcaaga gctgtgctgt tgcagtgaaa atagccttaa actgcatgtg gctaacctcg
     3421 ggataggtaa taccaagacg cactccgcgg tgacccatca tagggttgac ctcatgcagg
     3481 agttctcccc tctctttaat ctcgtcaatg gtgataccta gttcatgcgc caattctttc
     3541 tgtttttcaa gggtttgggg tacgaactca tgcaaggggg gatcgaggag gcggaatgtc
     3601 acgggcctgc cgttcataac acgcatcgtc tctcttgtgg catcaacaac aaaaggctca
     3661 agctccttca gagcatcaat cctctcttca agagaagagg cgtgaatcat cttgcggagt
     3721 tttgaaagtg gagcctcaga gtctttacca tagaacatat gttcaatacg gaagagacca
     3781 ataccttcgg caccaaattc aattgccttg agagcatcct caggggtgtc ggcattggta
     3841 cgaactccga gcgtacggaa tttatccacc atagtcataa actctacgaa acgaggattt
     3901 tcggaagcat ccactgtgct gactgctaca tcgtacacgt gaccctttgt gccgttgaga
     3961 gagaaaacat caccctcttt ataagtcttg ttgcctatag tcatcaccct cttattaccg
     4021 gaggttgtaa acttgatggc atcacagcct acaatacagc acttacccca cccacgggct
     4081 accagagcag cgtgagaggt catacctcca cgtgaagtca gcaatcctac agctgcgcgc
     4141 ataccttcta cgtcttcagg gttggtctcc tcacgcacaa ggattacctt ctcccctttc
     4201 gcagctgctt caattgcagc ctctgatgta aagacaagct tacctaccgc tcctccggga
     4261 cctgcaggaa gacctttagc aataactgta gccttctttt ctgcctcagg gtcaaggatc
     4321 gggtgaagta tctcgtcaag ctgcttgggg gcaactctca taactgcggt cttttcatct
     4381 atcattccct cttcaatcat atccatcgcc atatttagcg ctgcaaaacc ggttctctta
     4441 cctacacggc actggagcaa ccagagtttt ccatcctgga tggtaaactc aatatcctgc
     4501 atatctgaga aatgatgctc aaggtgatcc tggatatggt cgagctcctt gtatacttcc
     4561 ggcatcgcct tctcgagaga agccaaatgc ttgttgtgtt cattctttgt agattcattg
     4621 agagggttgg gggtgcgaat acctgccact acgtcctctc cctgtgcatc tacgagccac
     4681 tcaccataga aagtgtgctc accggtagcg gggttacggg agaatgctac gccggtggcg
     4741 gaattggatc ccatgttacc gaacaccatt gactgtacgt tgacggctgt accatattca
     4801 tcgggaatac cctcgatacg gcgataagct attgcccgct ttccattcca tgatttgaaa
     4861 accgcaccga tactgcccca gagttgtgct tgtgcatcat ccggaaattc ttttccaaga
     4921 acctctttta cacgcacctt aaagcggtcg cagaggtctt tgagatcctc ttcggtcagg
     4981 tcggtatcgc tcttatagcc ctttttctcc ttgaggtcgt gcatcatatt gtcgagctgt
     5041 acgcggatac ccttaccatc ctcaggctca atgccttcag acttctccat caccacgtct
     5101 gaatacatca ttatcagacg gcggtaagcg tcatatacga agcggggatt ttttgtcttt
     5161 tcgataagtc ccggaattgt ggcagagcaa agaccgatat tgaggactgt ctccatcata
     5221 ccgggcattg acatccttgc acccgagcga caggataaca ggagggggtt cttgctatca
     5281 ccaaacttca ttcccataac atcctcagta gcttttatag cttctgtaac ttcttcctta
     5341 agtccttccg gatacttgcc accaagtgca aaatactcgg tacaggtatc agtggtgatt
     5401 gtaaaacctg cgggaaccgg aatcccgaga gtacacatct ctgcaagatt agctccttta
     5461 cctcctagga ggtttttcat agtgccatta ccttcagcac tcttgccgcc aaagcggtaa
     5521 actctttttg tcatagataa ttaaatattt tattttttgt tgtttgacaa attgttattt
     5581 ttccattcat aaactgcaaa agtagtaaaa atataggaca atagcaatgc aatcactact
     5641 ttttttatac ctttgaaacc atgagaataa ttgtccattt agccatagct ctattgagtc
     5701 tctctctaat tgccggatgt aactcaaata gaagtagcaa agaaagttca aatagtatgc
     5761 ctgtattgag agttaaaaaa gtggttttcg actctgctaa gccctccgta gccgaagttg
     5821 ccgcaatgtt tgagagagaa aaaattgaat ttaaccccat agccgtcgtc aactggaaag
     5881 agtatggtta caaaccggac gtaaaattcc gcatagcaca ttcagcggaa gagatctatc
     5941 tccagtatgt cgtgaaggag gagggtgtca gagctacttt cggatacgat gccggatcga
     6001 aaccctacac cgattcctgt gtggaatttt tcatgattcc atccgacaaa gattccatct
     6061 attacaacct tgagatgaac tgtataggtc acggtacatt tgcaggaggt cctgaccgta
     6121 agcagcgcac ccgctttggt gatgaagtaa taagtcagat cagaagagag tcaactctcg
     6181 gcagtgaacc ctttggtgag cgtgccggtg aacaggagtg gactctcacc atagctatcc
     6241 caaagtccat ttactctctt tcagagttct ccgatttctc cggacgtaca gtcagaggta
     6301 acttttataa atgcggggat gaaatgcccg ttccacacta tctgagctgg catccgatac
     6361 cgattccctc tcccaatttc cataagcctg aacatttcgg acctatattt tttgagtaaa
     6421 agagtacgat ttgttctttt tttattacct tcgtgtgtta ctaaaatcct aattatgaaa
     6481 aaattgactc tattttcgct tgtagcagcg gctctgattt ccctttcagg atgtcagttc
     6541 atcagggatt tgataaacga ggagtatctt ctttacggcc agtgggatgt agcaaaagtt
     6601 tatgaaggcg agattgaact caattatgca gaaacgcgtt attcttttgt gtttgaaaaa
     6661 aatggaacgt tcaaattcca gatttgggag agtgcggcaa aaaccaatat ggtcattgcc
     6721 agctacacag gatcattctc ttacgacaaa gataagtaca ttctaaatat cagtttccct
     6781 atcgatgggc ttcttcaagg taaaaaagac ttcaatacaa acattgtttc acagttccag
     6841 gtcaaggaac tgactcttaa acgcctgctg cttgatgtca tagaggagga tgagcttgca
     6901 atattcaatc tcaactctac atgggaaatg aataaaaaat tctgaccatg aaaaaactat
     6961 acacttttct gctactttca ctcgttatgt tgacaagctg taaccctgag aaagagctga
     7021 agtccctcat atccgaacta gagggcaagc tactaccacg ctatgtggaa tcatcaaaaa
     7081 gttattgggc cggtactacg agcggcaatc ccgaa
//
LOCUS       DAOSIE010000050         7078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9657_length_7078_cov_3.775167, whole genome shotgun sequence.
ACCESSION   DAOSIE010000050 DAOSIE010000000
VERSION     DAOSIE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9657_length_7078_cov_3.775167"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /gene="rsgA"
                     /locus_tag="PLI69_02580"
     CDS             complement(<1..766)
                     /gene="rsgA"
                     /locus_tag="PLI69_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOG41410.1"
                     /translation="MENRSIATVVKHTGSHYLLSRLPEWDPFPAVIRGKIRLMGSTAT
                     NPIAVGDKVKYSPGDSEGNILATIEEVLPRTNYMIRRSVNLSRQAHIIAANLNRVFLI
                     VTIEYPEVKPAFVDRFLLTCEAYRIKVILVINKTDIYDEYALERMEEFKQIYSGAGYE
                     IMEVSALTGEGIEPIRELCKGESGDYVSLFSGVSGVGKSSLIRTIDPALDPKIREISQ
                     AHLQGRHTTTFYEMYPLSSGGFIIDTPGIRGFGLVDI"
     gene            879..1805
                     /locus_tag="PLI69_02585"
     CDS             879..1805
                     /locus_tag="PLI69_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG41411.1"
                     /translation="MEIKGEPESLYRPIEYMISVGGKRIRPSFCLTVFNLFSNNVGNN
                     IIYPAIALEIFHEFTLIHDDIMDKSDTRRNQPTVVKKWGINNAILSGDVMLILACKYI
                     SFCNSEVMPEAMRLFTETAVKVCEGQQLDMDFEEKSFITMADYISMIGLKTGVLIACS
                     AEMGALIAGVSGEVRKAIHQFGYQLGLAFQIADDYLDTFGDQQIFGKKIGGDIINNKK
                     TWLQVECIRRAEGADKERLTSILEMGEDMSDEKIAAMQQLYIDLGIRDDARDAILEYH
                     NRAFEAISGIGLTEAQTRQLHQFAEQLIYREK"
     gene            1822..3030
                     /locus_tag="PLI69_02590"
     CDS             1822..3030
                     /locus_tag="PLI69_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOG41412.1"
                     /translation="MAPAGNFESLMAAIQGGADSVYFGIGDLNMRSHSANNFASEELE
                     KVCNICDEHDVKSYLTLNIVLYDEDLEPMRETLEMAKKAGVTAVIASDMAAIAYARSL
                     GMEVHISTQLNVSNVESLRFHAEFADVIVLARELNLEQVAKIKEAIDRENIKGPSGRK
                     VRIEMFCHGALCMAVSGKCYLSLHEYAASANRGSCYQICRRGYRVMDLETGSELEIDN
                     KYIMSPKDLCTIEFIDKMIASGVTVFKIEGRARSSEYVKTVTRAYRDAADAVIDGKYT
                     PELAASLKNRLTTVFNRGFWDGYYQGARLGEWSDVYGNKATRTKSYIGKVTNYFSNIG
                     VAEVMVASGSFSVGDSLLITGPTTGVIEYEVSEIRVDMRNVGSAVKGDLCSIPVEEKL
                     RRGDKIYLFQ"
     gene            3196..4425
                     /gene="rocD"
                     /locus_tag="PLI69_02595"
     CDS             3196..4425
                     /gene="rocD"
                     /locus_tag="PLI69_02595"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOG41413.1"
                     /translation="MVITKNAQKFIDQEAKFGAHNYHPMPVVLSKGKGAFVWDVDGKK
                     YFDFLSAYSAVNQGHCHPKIVQALCDQAQDICLISRAFYNDCLGPYEEFATKFFGYDK
                     LLPMNTGAEAVETALKLARRWGYVKKGIPEDKAIIVVCNGNFHGRTITVISMSTDPDS
                     NSQYGPLTPGFIKIPYNDAEALEAVLEKDGAHVAAFLVEPIQGEAGVVVPDEGYLKKC
                     YDACKRHNVLFIADEIQTGIARTGKMICCEHENVRPDILILGKSLSGGVLPVSAALAD
                     DEVMLTIKPGQHGSTYGGFPLAAKVGIAALEVVRDEKLSERAEYLGRIFREELSKFKS
                     PFIKQVRGKGLLNAIVTTPINGKEAWDICLKMAEYGLIAKQTHRHIIRFAPPLVITEE
                     ELREAIEIIKKAFRELE"
     gene            4434..5360
                     /locus_tag="PLI69_02600"
     CDS             4434..5360
                     /locus_tag="PLI69_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HOG41414.1"
                     /translation="MSGISFRQTANRVLMVRPCSFGFNVQTASNNAFQRSGFESGAQE
                     AALREFDTFVALLRSHGITVDVIEDTPEPHTPDSIFPNNWFSTHSDGTLVLYPMFAPN
                     RRLERKQSVMDHLIGTFDIRNIYDLTPYESKEKYFEGTGSMVIDREAGIVYACRSPRT
                     DEDVLKEFCKLFDWDYFLFDAMDASGKAIYHTNVMMCVGTDLAVVCLESIRDLRVRED
                     FLRLLGKKDIVDISLEQMGSFAGNMLELQPFGERKPLLVMSSSARKSLRQEQLRMIES
                     HCDIASPDLRIIEANGGGSARCMLAEIFYEEF"
     gene            5347..6057
                     /locus_tag="PLI69_02605"
     CDS             5347..6057
                     /locus_tag="PLI69_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41415.1"
                     /translation="MKSFRFLFLFVVLLSPLPVALAQPTIYEEYAQEAGDALMIYRGR
                     KAIHYAFQFNGTFYWESPQFVAGDVIYNKKKYEGLLLNIDANFQQLLVKHPVSAITVA
                     LNSSYLTSFTMGEKRFLSLDGGIFEIIHEGPITLLRQITKKLMRDIHLRNGESIGYYD
                     PNFKENVFYYFKYNSTLYCLKDESLTQVRREKDILNLFPDRKKEIKAFMRKSSVSRRR
                     NFEEYCIKVLGYAESTLK"
     gene            6035..>7078
                     /locus_tag="PLI69_02610"
     CDS             6035..>7078
                     /locus_tag="PLI69_02610"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG41416.1"
                     /translation="MLKVRSNRFVVLSILLFLGLFVSGSLHAQHANVRLSHVSIDSLV
                     NFVRSIHPKTYFISDGLDQANYTVEAPREKILEKILSVLKEKGYAVTYFDEGIYILRT
                     VGFATELPMGYFSTGEAVQRDTSIYLDQSGYKFTFANKVYEIGESNSGVTGKVYLSGY
                     VTDVATGEPLVGISILEESTGTYAQSDASGFYRILLPVGEHLLRFSGYSLEDLDLNVL
                     LHDRGTLDVTMKVKVFALKGAVITSEGMAQHRTSRMGVERVRVENIKKVPAVFGEADV
                     IKVIMTLPGVKSVGEASGGFNVRGGANDQNLILFNDGTIYNPNHLFGVFSAFNTDIVS
                     DVELFKSSIPAEYG"
BASE COUNT         1893 a         1446 c         1740 g         1999 t
ORIGIN      
        1 ctatgtccac caatccgaag ccgcgaatcc ccggagtatc tatgatgaaa ccgccgctac
       61 tcagaggata catctcgtag aaagtggtgg tgtgcctgcc ctgtaggtgt gcttgtgata
      121 tttccctgat tttgggatcc agggcaggat caatggtgcg gatgagtgag gatttgccta
      181 ctcccgagac tcccgagaag agagagacat aatccccgct ttctccttta cagagttccc
      241 ttataggttc tatcccttcg cctgtcagag ctgagacctc cattatctca tatccggcgc
      301 cggaatagat ctgcttgaac tcttccatgc gttccagtgc atattcgtca taaatgtcgg
      361 ttttgtttat gactagtatg acttttatcc gataggcttc gcaagttagc agaaatctat
      421 ccacaaaggc aggttttacc tccggatatt ctattgtaac tatcaggaat acgcggttga
      481 ggttggctgc gatgatatgt gcctggcggg agagattgac cgagcgacgg atcatataat
      541 tagtgcgagg aagtacttct tctatggttg caagtatatt gccttcacta tcgcccgggg
      601 agtatttaac cttgtcgcct acagctatcg gattagtagc tgtggagccc atgagacgaa
      661 tttttccgcg gataaccgcg ggaaaagggt cccattcagg caggcgtgag agcagatagt
      721 gactgcccgt atgttttact actgtggcaa tagatctatt ttccatttgc gcaaatatag
      781 taatttgtcc aaaatccctt atatttgtat tctaaaaacg agcatttatg ctatctcacg
      841 aggatataga aaatattatt tccaggagta tatttaattt ggaaataaaa ggagaaccgg
      901 agtcgctcta ccgacctatc gagtatatga tctctgtcgg aggtaaacgg atcaggccaa
      961 gcttttgcct gaccgtattc aaccttttta gcaataatgt gggcaataac ataatttacc
     1021 ctgctattgc gcttgagata tttcacgagt ttacgcttat ccatgatgac ataatggaca
     1081 aatccgatac aagacgcaat cagcctacgg ttgttaaaaa atggggcatc aacaatgcaa
     1141 tcctttcagg tgatgtaatg ttgatattgg cttgcaaata tatatcgttc tgcaacagtg
     1201 aggtgatgcc ggaggcgatg cggctcttta cggagactgc ggtgaaggtg tgtgagggtc
     1261 agcagcttga tatggatttt gaggaaaaat cctttattac aatggctgac tatatctcta
     1321 tgataggtct taagacggga gttcttatag cttgttcggc tgaaatggga gcccttattg
     1381 caggagtttc cggagaggtt cgcaaagcca tacatcagtt tggctatcag ctaggcctcg
     1441 ccttccagat agcagatgac tatctggaca cttttggaga tcaacagatt ttcggcaaaa
     1501 aaatcggtgg cgatattatc aacaacaaaa aaacatggct tcaggttgag tgcatacgca
     1561 gggcggaagg ggccgacaag gagcgcctta cttctatttt agagatgggt gaggatatga
     1621 gtgatgaaaa gattgctgca atgcaacagc tctatatcga tcttgggata agggatgatg
     1681 ccagggatgc aatcctcgag tatcacaata gggcattcga agccatttcc ggtataggct
     1741 tgactgaggc tcagaccagg cagctccacc agtttgcgga acaactcatt tatcgtgaaa
     1801 aatagaggga atattgagat aatggccccc gcgggcaatt ttgagtcact tatggcagcc
     1861 atacagggtg gggctgactc ggtctatttc ggtatcggag atctcaatat gcgctctcat
     1921 tcagctaata attttgcttc ggaggagctt gaaaaggttt gcaatatctg cgatgaacat
     1981 gatgtcaagt cttatctgac tctcaatata gtactttatg atgaggacct ggagccgatg
     2041 agggagactt tggagatggc gaaaaaggcg ggtgtcacag cagtaattgc atcggatatg
     2101 gccgctatcg cttatgcccg ttctctcgga atggaggtgc atatctctac gcagctaaat
     2161 gtttccaatg tggagtctct ccgtttccac gcagagtttg cggatgtgat agtgctggcg
     2221 agagagttga atttggagca ggtagccaag atcaaggagg cgatcgaccg tgaaaacatc
     2281 aagggtcctt caggtcgcaa ggtacgaatt gaaatgtttt gccatggtgc tctctgtatg
     2341 gccgtttccg gcaaatgcta tctttctctc catgagtatg ccgcttccgc caatcgcggg
     2401 tcatgttatc agatatgccg tcgcggttac cgagttatgg atcttgagac gggatctgaa
     2461 ttggagattg acaataaata cataatgtcg ccaaaggatc tctgtaccat tgagtttata
     2521 gacaaaatga ttgcatcggg agtgactgtg ttcaagatcg agggaagagc ccgttcctcg
     2581 gaatatgtta agaccgttac ccgggcttac cgggatgctg cggatgcagt cattgacggc
     2641 aaatatactc cggaactggc cgcatctctc aagaatcggc ttaccaccgt attcaacaga
     2701 ggtttctggg atggatacta tcaaggagcg cgattgggtg agtggagcga tgtgtatgga
     2761 aataaggcaa cccgcaccaa gagctatata ggcaaggtta ccaactattt ttcaaatata
     2821 ggagtggcag aggtgatggt ggcatccggc tctttttcgg tcggggattc gttgctgatt
     2881 accgggccta ccaccggtgt catcgagtat gaagtctcag agataagggt ggatatgaga
     2941 aatgtcggta gtgcagttaa aggtgatctg tgctctattc ccgttgagga aaaactccgc
     3001 agaggcgata aaatctatct ttttcagtag atttttccta aaaatagcta tttcaacaga
     3061 ttccaggcaa ctttttacta acgatggaag tatttagtgc ccggcttcgt atattaactg
     3121 gtaagtttaa tattttttgt aactttgttg attgcaaatc aagtaaagta gttaacaaaa
     3181 aacagctcaa gaattatggt tatcacaaaa aatgctcaaa aattcataga tcaggaagca
     3241 aaattcggtg cacacaatta tcatccgatg ccagtggtcc tttcaaaagg caaaggtgca
     3301 tttgtatggg atgtagacgg aaaaaaatat tttgattttc tgtcagccta ctcggcagtc
     3361 aaccagggtc actgtcaccc aaaaatcgta caggccctct gcgatcaggc acaagatatt
     3421 tgtctcatat caagggcctt ttataatgat tgtctaggcc cttatgaaga gtttgcaaca
     3481 aaatttttcg gttatgacaa actccttcct atgaacaccg gagcagaagc ggtggaaacc
     3541 gccctaaaac ttgcccgcag atggggttat gtgaaaaagg gcattcctga agataaagct
     3601 attatcgtgg tttgtaacgg taactttcac ggccgtacga taactgtgat ttcgatgtcc
     3661 accgatcccg attccaattc acaatatggg cctttgactc ccggctttat aaagatacca
     3721 tacaatgatg ccgaagctct tgaggcagtt ctcgagaaag atggagcaca cgtggccgct
     3781 tttcttgtag aacccatcca gggcgaggca ggagtggtgg tgccggatga gggttatctc
     3841 aaaaaatgtt atgatgcttg caaacgccac aacgtgcttt tcattgccga tgagatacag
     3901 acgggtattg cccgtacagg caaaatgatt tgctgcgaac atgaaaatgt tcgccccgat
     3961 attctcattc ttggaaaatc cctctccggt ggagtgctgc ctgtttcggc tgcgctggct
     4021 gatgatgagg taatgctcac catcaaaccc ggtcaacacg gctccactta tggaggattt
     4081 cctcttgcag caaaggtggg tattgcagct cttgaagttg taagggatga aaaactttct
     4141 gaaagggccg agtatctcgg caggattttc agagaagaac tttccaaatt taagtctcct
     4201 tttatcaaac aggtgcgtgg aaaaggtctt ctcaatgcca tagttactac tcccatcaat
     4261 ggtaaagagg cttgggatat ctgtcttaaa atggcagaat acggtctcat agctaagcag
     4321 acacatagac atataatccg ttttgctccg ccccttgtga ttaccgaaga ggagcttcgc
     4381 gaggcgatcg agattatcaa gaaagcattc agggaacttg aataattagt tttatgtcag
     4441 ggatttcttt cagacaaacg gcaaatcgcg tactaatggt acgtccctgt tcctttggat
     4501 tcaatgtcca gacagcatct aacaacgcat tccagcgttc cggcttcgaa tcgggagctc
     4561 aggaagcagc attgagggag tttgacacct ttgttgcctt gcttcgttcg cacggaataa
     4621 cggtagatgt gatagaggat actccggaac ctcatacgcc cgactccatt ttcccaaata
     4681 actggttttc cacccattcg gatggcactt tagtgctata tccaatgttt gcacccaatc
     4741 gccggttgga gcgcaagcag agtgtgatgg atcatcttat aggcactttt gacatacgta
     4801 atatctatga cctgacgccc tacgagtcca aagaaaagta tttcgagggg accggcagta
     4861 tggtcatcga tcgcgaggcg ggcatagtat atgcctgccg ttctccccgt accgatgagg
     4921 atgtactcaa ggagttttgc aaactgtttg attgggatta ttttctgttt gatgctatgg
     4981 atgcatccgg gaaagccatt tatcatacca atgtaatgat gtgtgttgga actgatctgg
     5041 cagttgtctg tctggagagt atcagagatc ttcgggtaag ggaagacttt ctacgcttgc
     5101 tgggtaagaa ggatatcgta gatatttcac tggaacaaat gggcagcttt gcgggcaata
     5161 tgctggagtt gcagcctttc ggagaaagga aaccattact agtgatgtct tcttctgcaa
     5221 gaaaatcgct tcggcaggaa caattgcgta tgatagagtc ccattgcgac atagcttccc
     5281 ccgatttaag gataatagag gccaatggtg gaggttctgc ccgctgtatg cttgctgaga
     5341 ttttttatga agagttttag gtttttgttt ctgtttgtcg tgctgctctc tcctctaccg
     5401 gtagcattgg cacagcctac aatttacgaa gaatatgctc aggaagcggg tgatgccctt
     5461 atgatatata gggggcgcaa ggcaattcac tacgcttttc agtttaatgg aaccttttac
     5521 tgggaatctc cgcaatttgt tgcgggagat gttatctaca ataagaaaaa atatgagggt
     5581 cttctgctca acatagatgc aaattttcag cagttgttgg taaagcatcc tgtcagtgcc
     5641 attaccgtag cgctcaatag ctcttatctg acttcgttta ctatgggtga gaagagattt
     5701 ctctctctgg atggaggtat tttcgagata attcatgaag gacccattac tcttttgcgg
     5761 cagattacca aaaagcttat gcgtgacatt catctgcgca atggggagtc gattggctat
     5821 tacgacccca atttcaaaga gaatgtattt tattatttca aatacaactc tacattgtat
     5881 tgtctcaaag atgaatctct gactcaggtc agacgtgaaa aagatattct gaatctcttc
     5941 cctgaccgta aaaaggagat aaaggcattc atgaggaagt cgagtgtttc tcgcagaagg
     6001 aattttgaag agtattgtat taaagtatta ggttatgctg aaagtacgct caaatagatt
     6061 tgtggtactc tccattttgt tgttccttgg tctctttgta tcggggagtc tgcatgccca
     6121 gcatgctaat gttcgtctaa gccacgtcag catcgattct ttggtaaatt ttgtgaggag
     6181 catccacccc aagacctact ttatctccga tggtctcgac caggccaact atacggttga
     6241 ggccccgcgt gaaaagattc tggaaaagat actttcggta cttaaggaga agggctatgc
     6301 agttacctat tttgatgaag gcatctatat actcagaaca gttggatttg caaccgaact
     6361 ccccatgggt tatttcagta ccggcgaagc ggtgcagcgc gatacttcca tctatctcga
     6421 ccaatcgggt tataaattta cgtttgccaa taaggtatat gagattggag agtccaacag
     6481 cggagtcacc ggtaaagtct atcttagcgg ttatgtcacc gatgtggcca ccggagaacc
     6541 tttagtgggc atttcaatcc ttgaagagag cacaggcacc tatgctcaaa gtgatgcgtc
     6601 cggattttat aggatattgc tgcctgttgg agagcacctg cttaggtttt caggctattc
     6661 tcttgaagat ctggatttga atgttctgct tcatgataga ggtactctgg atgtgacgat
     6721 gaaagtaaag gttttcgctc ttaaaggagc agtcattaca tccgaaggaa tggcccaaca
     6781 tcgtaccagt cgcatgggtg tggagagagt acgtgtagaa aatattaaaa aggtgcctgc
     6841 ggttttcggc gaggccgatg tcataaaagt gatcatgacg cttcccggcg tgaaatcggt
     6901 aggtgaggcc tccggcggat ttaatgtgcg cggtggagca aatgaccaaa atctcattct
     6961 ttttaatgac ggtacaatct acaatcccaa tcaccttttc ggtgtattct ctgctttcaa
     7021 tactgatatt gtgagtgacg tagaactatt taagagttcc attcccgccg aatatggc
//
LOCUS       DAOSIE010000051         6990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9806_length_6990_cov_3.339149, whole genome shotgun sequence.
ACCESSION   DAOSIE010000051 DAOSIE010000000
VERSION     DAOSIE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9806_length_6990_cov_3.339149"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLI69_02615"
     CDS             <1..206
                     /locus_tag="PLI69_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41417.1"
                     /translation="ALDSCIAKHEIPWIQLCNYVKWKENPVSAAYDLHWIPTMFLIDT
                     EGKVIGFAFTAEALSSLLSEKIH"
     gene            complement(223..1173)
                     /locus_tag="PLI69_02620"
     CDS             complement(223..1173)
                     /locus_tag="PLI69_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOG41418.1"
                     /translation="MEYFILVISLLAIAFGANRFVDGAEGIARRLKISDFVIGAVVVG
                     FGTSFPELLVSLKGAISGNADVAIGNVVGSNIFNVLGILGITAMLFPIPTTRENIRRD
                     APFCFAVSILLILLAFNFFRGGETVISRVDGVVLLAVFALFLWISLRQGNQASSSDED
                     TKTHNGKPLWHSALISIVGLVVLILGCNFFVEQAVIIARNLGVSDAYISITLIACGTS
                     LPELAASVAAAFKKNTQMALGNIIGSNIFNITFILGTSSLIRPLYSPDIVLTDYLVMA
                     GAALLLMVFATTKVKISRWEGAFMFAAFVAYNIWLLGMQG"
     gene            complement(1185..1628)
                     /locus_tag="PLI69_02625"
     CDS             complement(1185..1628)
                     /locus_tag="PLI69_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41419.1"
                     /translation="MDNLFVKTRSTKDIIISTVLLVAGVTMVALPTPASVNIAGLFMA
                     CTGIVLLAVLKSSWKNTETREKFSSKTLYFPREMETKLKEAMEGSLKKITSDTTSQSS
                     ALKLDILYNKKTGKAFCQLSEYIPYQYHPFTDMIQKPVDEVLHLV"
     gene            complement(1737..2150)
                     /locus_tag="PLI69_02630"
     CDS             complement(1737..2150)
                     /locus_tag="PLI69_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41420.1"
                     /translation="MKWIGRILLALIAPLLLFIAARSGHVVMDQIHFPDEVTTLADHN
                     DKLPLPEFDCRCDLMLPVSTTGVPGIHFQQVAGSTGNSQGHSRQTDPFKRYSILLYGR
                     IRSCGFVREHNISFLAASSLMDSEARRISNRILII"
     gene            2263..2586
                     /gene="rnpB"
                     /locus_tag="PLI69_02635"
     ncRNA           2263..2586
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLI69_02635"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2602..3444
                     /locus_tag="PLI69_02640"
     CDS             2602..3444
                     /locus_tag="PLI69_02640"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HOG41421.1"
                     /translation="MRYILSILVLFCTITTSCSKPQVRFTPEAKDSFERLLEQMLPHK
                     ALPMDQLVIKTALQKLGTPYVAGTLEEDPEMLTVHMDKTDCILFVEMCLAFSLTLKEE
                     NPDFDSYCDNLQKLRYRNGIVEDYASRIHYTSEWIRQAAERGIMEEITAEIGGVVWPQ
                     EFSFMSTHTGSYRQLRENPSLVQKIRATEEYLNGFEYHYIPKDEISACEHLIHDGDII
                     CFNSRAKGLDIAHVGYAYWQEGRLTFIHASSVEMKVVINKKPLTEYISGIKSNNGIRV
                     VRLK"
     gene            complement(3469..4917)
                     /locus_tag="PLI69_02645"
     CDS             complement(3469..4917)
                     /locus_tag="PLI69_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOG41422.1"
                     /translation="MANIKFFSGEEIPLELHKVRIVQKLNLVPIERRRDAIKDAGYNT
                     FKLTTKDVFLDMLTDSGTNAMSDNQLAAMMRADDAYAGSESFERLQKGVEDVLGKKYL
                     LPAHQGRACENIIAKAFIKPGQIVLTNYHFTTTLAHITEVGGRVIELLHDEALVLEST
                     NPFKGNIDIEKLVAAIEENGAENIAFIRMEASTNLIGGQPFSIRNMMDVRKVADQYNI
                     MLVLDASLLGENAYLVKQREEKWKENSMADIVKMMTGLADLVYFSARKLTSSRGGGIC
                     TNVRELYMTMEPLIPLFEGFLTYGGMSIREIEAMAVGIYETIDETMISQSVSFIKYFV
                     ENAVKAGIPMVTPAGVLGAHVNAGKFCEHIPQHEYPAGALAAAFFLVSGVRGMERGTV
                     SSVRDENGNEIPADAELLRLAFPRRVFTLSQTMYALDRLTWLYENRHLIGGLKFTYEP
                     PVLRFFMGELEPLTDWPSRLVEKFRKDFGNSL"
     gene            5204..5276
                     /locus_tag="PLI69_02650"
     tRNA            5204..5276
                     /locus_tag="PLI69_02650"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5237..5239,aa:Thr,seq:cgt)
     gene            5332..6588
                     /locus_tag="PLI69_02655"
     CDS             5332..6588
                     /locus_tag="PLI69_02655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG41423.1"
                     /translation="MAIVMEFFERGAYYGVLSVLSVYLVAGAMDGGLGFSRMQAGTIM
                     GTIQPLLYFLPIVAGGIADRYGYRKVLFFAFGCITAGYALTGVFTDYGLVFASLILMA
                     IGAGFFKPVISGTIARSTDETNSTLGFGIFYWSINLGAFLFPLFLIPNLKAMGYEYIF
                     YMAAVVGVILLLINTFFYKEPISLRDTGSAQKSVGETLLGILTVLKDFKFIIFILLYS
                     GFWILYFQMYSTVLWYLRDYMDMTPVNNAVNSFLRLFVDNPSWVFDTEHVTVVNAGVI
                     ICLQLFVSKIVAKTRPLPTMITGLSLGTLGMLILSISSQPWVFITGIMVFTIGEMTTH
                     PKFLSYIGMISPPDKKALYMGYSFLYGVIGSSIGSFLGSALYVKFVDEMGRPGQLWAL
                     LSIIGVVSIIGLLIYNKVVVKSKATA"
     gene            6678..>6990
                     /locus_tag="PLI69_02660"
     CDS             6678..>6990
                     /locus_tag="PLI69_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41424.1"
                     /translation="MGKRFIALLMAIVISLSFSGCETLRTSDKNGVFINERLHAETKS
                     AFIGIQMYERLHVLRFYEDYYSESPYLPSSSVKEKVVIYGYLDVNWHTGGCLISREYI
                     RN"
BASE COUNT         1903 a         1565 c         1579 g         1943 t
ORIGIN      
        1 aagcgttgga tagttgcata gcgaagcacg aaatcccatg gatacaactc tgcaattacg
       61 tcaaatggaa agagaatccg gtttcggcag cttatgacct ccactggatt cccaccatgt
      121 ttctaataga tactgaaggt aaagttattg gttttgcatt taccgctgag gctctctcat
      181 cattgctaag cgagaagata cactgattgt aaagagcagc gatcaaccct gcatgccaag
      241 tagccaaata ttgtaagcga cgaaggcggc aaacataaat gctccctccc atcgtgaaat
      301 tttgactttt gtagtggcaa agaccatcaa tagaagtgcc gcacctgcca ttaccagata
      361 atccgtaagc actatgtccg gactataaag cgggcggatc agagagctgg ttcccaatat
      421 aaaggtgata ttgaagatat tggagccgat gatgtttcca agagccatct gggtgttctt
      481 tttgaaagct gcggcaacgg atgccgcaag ttccggaagc gaagtaccgc aggcaatgag
      541 ggtaattgaa atataagcat cacttactcc caggtttcgg gctataatca ctgcctgttc
      601 tacgaaaaaa ttacagccca atatcaaaac cacaagccca acaatggata ttaaagcact
      661 atgccacaaa ggtttgccat tgtgtgtctt tgtgtcttca tcacttgatg aagcctgatt
      721 accctgtctg agagatatcc acaagaaaag ggcaaatact gcaagaagca ccactccatc
      781 tactctggat atgacagttt ctccacctct aaagaaatta aaggccagaa gaatcagcaa
      841 tatacttaca gcaaaacaaa agggagcatc ccttcgaata ttttccctgg tggtcggtat
      901 cggaaacaac atcgcagtta ttcccaaaat gccgagtaca ttaaagatat ttgagccgac
      961 tacattacct atggcaacat cggcattgcc ggaaatggct cccttaagac ttacaagcag
     1021 ttcaggaaag gaggtgccaa atcccaccac aaccgcccca attacgaagt cggagatttt
     1081 taagcgccgt gcaatgcctt cggcgccatc cacgaaacga ttggcgccga aggctattgc
     1141 aagaagcgat attactaaaa taaaatattc cattccaatg tcattcagac aaggtgaagt
     1201 acttcatcca ccggcttttg gatcatgtcg gtgaagggat ggtactggta aggtatgtat
     1261 tctgagagtt ggcaaaaagc cttgcctgtt ttcttgttat aaaggatgtc cagtttcagt
     1321 gccgaagact ggctggtggt atccgaagtt attttcttga gggaaccctc catagcttct
     1381 ttcagcttag tctccatttc cctgggaaaa tagagggttt tagaggagaa tttttcacgg
     1441 gtttcggtgt ttttccagct ggatttgagt acagccagca ggacgattcc cgtacaggcc
     1501 ataaagagac ctgctatatt gactgatgcc ggtgtgggaa gagccaccat tgttactcct
     1561 gctaccagaa gaaccgtgga tatgattatg tcttttgtag atctggtttt tacaaacaaa
     1621 ttgtccattt tgtattcttt ttttttggat tggataatat aataaaagtg tgggaaagtt
     1681 atgattagta cattcccaaa attaccctta gggtattcat ggaagtatcc aatgcactaa
     1741 attataagaa tacgatttga aatgcgtctt gcttccgaat ccataagtga tgatgcggcc
     1801 aagaatgaga tattgtgttc cctgacaaaa ccgcaggaac ggattctgcc ataaagaagt
     1861 atcgaatagc gtttaaaggg gtctgtctgc ctgctatggc cctgagagtt accggttgag
     1921 cctgctacct gttggaaatg tattcccggt acgccggtag tgcttacagg caacatcaga
     1981 tcacatctgc aatcaaactc aggcagaggc aatttgtcgt tatgatcggc aagagttgtg
     2041 acctcatcag ggaaatggat ttgatccata acgacatggc cgctcctcgc agctataaaa
     2101 agcaacagag gagcaataag cgccagcaat attcttccga tccatttcat ccccgcaaag
     2161 atagaaataa agattcactt aaaagataag tagacaaaat catttaattc atgatttgtt
     2221 tctcaaagac cttttctaaa atatattcct atctttgtac cgcaggtgag acttcctatc
     2281 gctctttcgg gagaggaaag tccggacagc acagagcgct tccctgtgga aagcacagac
     2341 agatggaagt ttgtggagcg cgtaacagaa aacaaccgcc cgcaagggta agggtgaaaa
     2401 tgtgaggtaa gagctcacaa ccgtcgatgg tgacaccggc ggggtacgca ccggaaggct
     2461 gcaagaccaa atataccggc aattaagggt tgctcgcccg ctgtcggggg gtaggtcgcg
     2521 gtagataaat gataggaacc tttaagataa taaggtacag aatccggctt atgtctcacc
     2581 ttttttgata attcgattta tatgagatac atactttcaa ttttggtgct tttctgcacc
     2641 attacgacat cctgcagcaa gccgcaggtc cgattcactc cggaggctaa agactccttt
     2701 gagcgactgc ttgaacagat gctgccccat aaggcgctcc ctatggatca gttggtaata
     2761 aaaaccgctc ttcagaaact cggcactccc tatgttgccg gcactcttga agaggatccc
     2821 gagatgctta ccgtccatat ggacaaaacc gattgtattc tctttgtaga gatgtgtttg
     2881 gcattctcac tcaccctcaa agaggagaat cccgactttg actcctattg cgacaatctg
     2941 caaaaactcc gctaccgtaa cggcatcgtc gaagactacg cttcacgtat ccattacact
     3001 tcggaatgga tcaggcaggc agcggaaagg ggtattatgg aggagattac cgcagagata
     3061 ggaggtgtcg tgtggccgca ggaattctcc tttatgagta cccataccgg atcttaccgc
     3121 caactcaggg aaaatccctc tctggtgcaa aagatacgag ctactgaaga gtatctcaac
     3181 ggatttgaat accattacat cccaaaggat gaaatctcag cctgcgaaca tctcatacat
     3241 gacggtgaca tcatctgctt caacagtagg gccaagggac tcgatatagc ccatgttggc
     3301 tacgcctatt ggcaggaggg ccgattgacc tttatccacg cctcttcggt cgaaatgaag
     3361 gtggttataa ataaaaagcc gctgacagag tatatcagcg gcataaaaag caataacggg
     3421 atcagggtcg tgcggctcaa ataagcctat caaaagcaac aagctccgct acagactatt
     3481 gccgaaatcc ttcctgaact tctccacaag tctcgaaggc cagtcggtga gaggctccaa
     3541 ttctcccata aagaatctca acacaggtgg ctcgtatgta aacttcaagc caccgataag
     3601 gtgcctgttt tcatatagcc atgtaaggcg atcgagcgca tacattgtct gtgagagtgt
     3661 aaataccctg cggggaaatg caaggcgcag caactcggca tccgcaggaa tctcattgcc
     3721 attctcatcc cgaacacttg agaccgtacc tcgctccata ccccttactc ctgagaccag
     3781 gaaaaaggcc gcagcaagtg cacctgcagg atattcatgc tgcgggatat gttcgcagaa
     3841 tttaccggca ttgacatgcg ctccgagtac acccgccgga gttaccatcg ggatgccggc
     3901 cttcaccgca ttctccacga aatattttat aaaacttaca ctctggctta tcatcgtctc
     3961 gtcaatggtc tcatagatac ccaccgccat agcctcgatc tccctgatgg acattccacc
     4021 gtaggttagg aatccctcaa agagcggtat cagcggctcc attgtcatat agagttccct
     4081 cacattggta cagataccgc ctccacgcga tgaggtgagt ttcctcgcag agaaatatac
     4141 gaggtcggca agtccggtca tcatctttac aatatccgcc atactattct ctttccattt
     4201 ctcttcacgt tgcttaacca agtatgcgtt ttcaccgagg aggctggcgt caagtacaag
     4261 cataatgttg tactgatcag ctaccttgcg cacatccatc atgtttctga tggagaaggg
     4321 ttgtcctccg atcaggttgg tggaggcctc catacggata aaagcgatat tttcggctcc
     4381 attttcttcg atcgcagcca caagtttctc tatatcaata tttcctttga atgggttggt
     4441 ggattcgagt acgagcgctt catcatgcag gagttcgatt actcggccac ccacttcagt
     4501 tatatgcgcc agtgtggtgg taaaatggta gttggtaagc actatctgac ccggcttgat
     4561 aaaagccttg gctatgatgt tttcacaggc gcgcccttgg tgagcaggaa gaagatattt
     4621 tttcccaaga acatcctcta cacccttttg tagacgctcg aatgactcgg aacccgcata
     4681 agcgtcatcg gccctcatca tggctgccag ctggttgtca ctcatcgcat tggtaccgct
     4741 gtcggtgagc atatccagga agacatcctt tgtagtgagt ttgaaggtgt tgtagcccgc
     4801 atccttgata gcatcacgac ggcgctctat tggcacaaga ttgagttttt gcacaattct
     4861 taccttgtgg agttccagag ggatctcctc tcctgagaaa aatttaatgt ttgccataat
     4921 tgtgtgtttt tgttgattat tattgtttct tgattttttt caaaaaaaaa cccgctccaa
     4981 aaggaccggg aaatgaaaca ggaattgaat acaaaaaggg tactatcccc ggtcggtgga
     5041 acggcggtaa tagtagtagt aatagctgtt tgtaaaaaat ctgttcatct caaatataga
     5101 cacttctacg cagtgttgcg gacaaaggta agtaaaataa tgtgaagttg ttcacctaaa
     5161 aatctttttc tttaaaaaat ttgtattttt gcagccgcaa aaggcctttt tagctcagtt
     5221 ggtagagcgg ctcattcgta atgagcaggt cgcgggttcg agtcccgtga aaggctcaaa
     5281 gatttatctc taagattcat tatgagaagc aaatttcaaa gaaacttttg ggtggcaata
     5341 gtaatggagt tcttcgaaag aggggcttat tatggcgtat tgtcggtttt atcggtctat
     5401 ctggttgcag gtgcgatgga tggcggtctc ggtttttcga ggatgcaggc aggtactatc
     5461 atggggacta tccagcctct tctctatttc cttccaattg ttgccggagg gattgcagat
     5521 cgctatgggt accgcaaagt actctttttt gctttcgggt gcatcacggc aggttatgct
     5581 ttgaccggtg tatttactga ttacggtctc gtatttgcct cgctgatact tatggcgata
     5641 ggtgcaggat ttttcaagcc ggttatcagc ggaacaatag cccgatccac agatgagacc
     5701 aattccaccc tcggtttcgg tatattctac tggtcgatca acctgggggc cttcctcttc
     5761 cctctctttc tgattcccaa cctcaaagct atggggtacg aatatatttt ctatatggct
     5821 gcggttgtag gagtcattct attgcttata aacacctttt tctataaaga acccatttcc
     5881 cttcgtgata cggggagtgc gcagaagagt gttggagaga cactcctcgg catccttacg
     5941 gtacttaagg atttcaaatt tattatcttc atactgctat attcgggatt ctggatacta
     6001 tatttccaga tgtacagtac cgttttgtgg tatctgaggg attatatgga tatgactccc
     6061 gtcaataatg ccgtcaactc atttctgaga ttatttgtgg ataatccatc atgggtattc
     6121 gataccgagc atgtaactgt agttaatgcg ggtgttataa tctgccttca gcttttcgtc
     6181 tcgaaaattg tagccaagac ccgtcccctt cctaccatga ttaccggtct ctctttaggt
     6241 acgctgggca tgctgatttt gagcatttcc tcacaaccgt gggtattcat aaccggaatt
     6301 atggtcttta ctatcggaga aatgaccacc catcccaagt tcctttcata cataggtatg
     6361 atatcccctc ccgacaagaa agctctttat atgggttatt ccttcctata cggagtgata
     6421 ggcagctcaa tcggctcttt cctcggttct gctctatatg tgaaatttgt ggatgaaatg
     6481 ggccgtcccg gccagttgtg ggcgcttcta tccattattg gagtggtcag catcatcggg
     6541 ctgttgattt acaacaaagt tgtggttaag tccaaggcaa ccgcataaca tccaacaata
     6601 aatactccga gtttcagaaa ttcttttgtg caattttaac ttatgagtaa atttgtacaa
     6661 aataggggaa ttatgttatg gggaaacgtt ttattgcatt gttgatggct attgttatct
     6721 ccctttcttt cagcgggtgt gaaacattgc ggacatcgga taagaacgga gtatttataa
     6781 atgaacgttt gcatgcagag acaaagtccg ctttcatcgg cattcagatg tatgaaaggc
     6841 ttcatgttct tcgtttctac gaggactatt actctgaaag cccttatttg ccctcttcat
     6901 cagtaaagga aaaggttgta atatatggtt acttagatgt aaattggcac acaggcggct
     6961 gcctaatttc aagagaatac attcgcaata
//
LOCUS       DAOSIE010000052         6894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_9989_length_6894_cov_4.594970, whole genome shotgun sequence.
ACCESSION   DAOSIE010000052 DAOSIE010000000
VERSION     DAOSIE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9989_length_6894_cov_4.594970"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>218)
                     /locus_tag="PLI69_02665"
     CDS             complement(<1..>218)
                     /locus_tag="PLI69_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41425.1"
                     /translation="LLQGTASLEFWQTYTYQEIQPFLIELNNEVRVRIEAAEADAAVA
                     EPEESVDSELKSIEEELAAATGEVSTGT"
     assembly_gap    219..228
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<229..966)
                     /locus_tag="PLI69_02670"
     CDS             complement(<229..966)
                     /locus_tag="PLI69_02670"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOG41426.1"
                     /translation="MQSKGAITFVAILIGLACIFQLSFTAATYIQENKASKYAEEVVL
                     AEQSKPSFSEISELDKAFYLDGIRTEAKRVYLDSIANKKIYLGYTYKEVKEKELNLGL
                     DLKGGMNVMLQLDMAELVRSCAREKTTPEFEQVMALARARAAETHTDFITLFAEAWDE
                     VAPNQRMSFDIDLDRLSGDIRNKSRVTNEEIITLLQQEADAVINNSFTVVERRINQFG
                     VAQPNIQKIARTGRILVELPGVKEPERV"
     gene            1085..2953
                     /locus_tag="PLI69_02675"
     CDS             1085..2953
                     /locus_tag="PLI69_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41427.1"
                     /translation="MRYRCIDRAFFLILTLFAATSLMGQDWGVEMRRAQALHESYDFD
                     KALDIWHSLLERSTDSLQRIDLLERIVRSENGRNMLSYATEPRVLSHLTVPRSDFFLW
                     YSHLEDKSWLPIPNAFVPNSASHHQYYTSIYFPEGTRNIVFSSQDESGSWNLYTSTCS
                     GDTLWSHPTLINENLTSPEDDIFPILSRNGKELYFSTKGLFGMGGYDLFVSRWDEEIN
                     DWGMPENLGFPFSSPFDDFLFSDTPDGNFTLFASNRNCHRDSMTIYVLEFENKPIKKP
                     VTSLEQARNLAALLPTVEEPLSAPEESKELPIAPAVDSLFLNYTSLLATMHALQDSLA
                     TLGKDLKMMRELYTSTENEQELSSLSRLLLDTEHRSIVLQSEINRVSGALQQMEMNFL
                     MDGIIINLEDISEEPQETSSPEHKQYTFAKLNPAQPFDIPIEVPKESFDYSFRILDTA
                     VIVEPVITPGVSYSIQLYVTSHKVMLHQLKGLSPVFEKRQSSGKYLYTAGIFHTYNEA
                     LSQINIVKKRGFPSAILVAYLDGKSISVHEARKAEKMPVAGPKYRIVLETFSEGIPDA
                     IMAIIRNNTSKEVIRSISEGKVLYIIAPFDEKEEAEAFATILKGNGVEGITTETIK"
     gene            2963..3430
                     /locus_tag="PLI69_02680"
     CDS             2963..3430
                     /locus_tag="PLI69_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOG41428.1"
                     /translation="MGTGLIIMLIVLGIILILVEILLIPGFAVTGVLGVGSLVLASYF
                     GFTHLGTRAGIIIIVVEVIVLSVLLYYALQAKTWKKLSLHTNIDAKVDEHPETKGLTS
                     GMEGISTTRLNPMGRAKIGGFDLEVRSIEGLIDERTEIVIDSIEEEKIFVKKK"
     gene            3451..4416
                     /gene="floA"
                     /locus_tag="PLI69_02685"
     CDS             3451..4416
                     /gene="floA"
                     /locus_tag="PLI69_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653914.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOG41429.1"
                     /translation="MDGITSVFIWIGVAIVGLIILLWFFPITMWFQALISGVRISLLQ
                     LILMRWRKVPPGTIVMAMITGTKAGLTLNANELEAHYLAKGNVPKVVNALISADKANI
                     ALNFKMAAAIDLAGRDVFEAVQMSVNPKVINTPPVTAVAKDGIQLIVKARVTVRANIK
                     QLVGGAGEETVLARVGEGIVSSIGSSESHKEVLENPDSISKVVLNKGLDAGTAFEILS
                     IDIADIDIGKNIGAVLKIDQANADKNIAQARAEEKRAMAVAVEQEMKAKAQEARARVI
                     EAEAQVPLAIAEAFRSGNLGIMDYYKFQNIKADTDMRESISKGSK"
     gene            4491..>6894
                     /locus_tag="PLI69_02690"
     CDS             4491..>6894
                     /locus_tag="PLI69_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOG41430.1"
                     /translation="MDSFIKKLYSRVSVLAIFLIVSTLSSAQNSPDGSLEWYLPDGEH
                     QFNTSIPTPEQVLGYQISTYFLNWGDVCKYMERLEAASDRVSLRRFGRTYNYREFIQI
                     YISSEKNISNLEKIREEHLLLCDPTRSSLLETDKMPVIVNLMASVHGNETSGVNAAML
                     AAYFFAASDSPQIKEILENTIVVITPGLNPDGISRFAHWTNSTASKNKRADINGPGQM
                     EPWPSSRTNHYYADCNRDWLSVQHPEGINSVTMYKWWMPNVVLDMHEQGTTSGFFFSP
                     GDPNRTYSGISQANQDLTLKIAKNTAIELDKKGLLYFSKESYDDFYIGKGASYGDLQG
                     SICILHEQGASYGYLRNTPWGILTFPETIRTQTLAAISVVKSGFQMREELLSYQKNYF
                     IDQLKTAQKDPIAGVRFAVKGDRAREYHLLEILLTHDIEVYRDNKEPGYIVPIVQNRY
                     YTFKAMWDHITSFKDSVFYDISTWSFPSAFGIESENLKSIQGRLSERVTKAIFPKGGI
                     SGGISHIGYAFSNNEYYAPKMASELLKEGFFLRVAGKSFTSLEDNREYTFPAGTIFMP
                     VIGQPADKEALYERISALAEQTGVQVHSFKSAMMKDYDLGSPIYSSLKKPDVAIITGR
                     GLSVSNIGEAWLSIDRRFEFDHTVIDHSRITSSRDLSSYNVMVLLGGVPSPLQQGDFY
                     ETLGSWVRQGGTLILGGDAAPIAQKAGLCEIVTTTGKTISGVILNCEIDLESPLFWGF
                     SENALQVFKQKATVYSCEKGSTPMKYSKDPYISGYTTPEIIDNFRGGQAIITHKVGKG
                     RLI"
BASE COUNT         1911 a         1613 c         1535 g         1825 t
ORIGIN      
        1 aagtgccggt acttacctct ccggttgctg ctgcaagctc ctcttcaatg ctcttaagtt
       61 cgctatctac agactcttcg ggctcagcta cagctgcatc agcctcggct gcttcaatgc
      121 gcacacggac ttcattgttg agttcaataa ggaagggctg aatctcctga tatgtatatg
      181 tctgccaaaa ttcgagagat gctgtaccct ggagaagtnn nnnnnnnnta cacgctcagg
      241 ctcttttact ccgggaagtt ccacgaggat tctacctgta cgggcaatct tctgaatatt
      301 gggctgtgct actccaaact ggtttatacg tctttctact acggtgaagg agttgttgat
      361 aacggcatct gcctcttgct gaagcagagt aatgatctct tcattggtaa ctctggattt
      421 gttcctgata tcaccgctga gcctgtcaag gtcgatgtcg aatgacatcc tctggttggg
      481 agccacctca tcccatgcct cggcaaagag ggtaatgaaa tcggtatgag tctcagccgc
      541 cctggctctt gcgagtgcca tgacctgttc aaactccgga gtggtctttt cacgtgcgca
      601 ggagcgtacc aactcagcca tatcaagctg gagcatcacg ttcataccgc ccttaaggtc
      661 gagacccaga ttgagctctt tctctttgac ttccttgtag gtgtaaccga ggtaaatctt
      721 cttgttggct atggaatcga gatatactct cttagcctct gttcttattc cgtcgagata
      781 aaatgctttg tccagctctg atatttcgct gaacgaaggc ttggactgct ctgcaagaac
      841 cacttcctct gcatatttgg aagctttatt ctcctggatg tatgtagccg ctgtgaatga
      901 tagctggaaa atacaggcta gtccaatcag aattgccaca aatgtgatgg cacctttact
      961 ttgcatactt ttatatttta aagttattta ttctccttat attccgaaaa gaagggcaaa
     1021 attacgatta ttttttaata tataaagtat ttcctatttt tgtttggttt tttcggaagt
     1081 gattatgaga tacaggtgca tagacagggc tttttttcta attttgacac tttttgcggc
     1141 tacaagcctc atgggtcagg attggggagt ggaaatgcgg cgtgcccagg cattgcacga
     1201 aagctacgat tttgacaaag cactggatat ttggcattcg ctcctcgaaa ggagcacgga
     1261 ttctcttcag cggatagacc tgcttgagag gattgttcgc agcgaaaatg gacgcaatat
     1321 gctaagttac gcaaccgaac cgagagtcct ttcccatctc accgtacctc gcagcgattt
     1381 ttttctatgg tactcacatc tggaggataa atcctggctt cccattccca atgcttttgt
     1441 ccccaatagc gcctcccatc accaatatta tacatccatc tattttcccg aagggacaag
     1501 gaacattgta ttctcgtcac aggacgaaag cggctcctgg aatctctaca cctccacctg
     1561 ttccggagat actctctgga gtcatccgac acttataaac gaaaatctta catcccccga
     1621 agatgacatc tttcctattc tgagccgtaa cggcaaggaa ctctacttct ccactaaggg
     1681 tctctttgga atgggcggat atgacctctt cgtcagcaga tgggatgagg aaataaatga
     1741 ctgggggatg ccggagaatc tcggatttcc tttttcttct ccattcgacg attttctatt
     1801 cagtgatact cctgacggca attttaccct attcgcctcc aatagaaact gccataggga
     1861 cagcatgacc atctatgtgc tggagtttga aaacaaaccg atcaaaaagc ctgttacttc
     1921 cctggaacag gccagaaatt tagctgcgct attaccaacg gtggaggagc ctctttcagc
     1981 accggaagag agcaaagaac tccccattgc cccagccgtg gactctctct ttctgaacta
     2041 cacctccctg ctggccacta tgcacgcatt gcaagattct ctggctaccc tgggaaagga
     2101 ccttaagatg atgagagaac tttacacctc tacggagaat gagcaagaac taagttcgct
     2161 ctcaagactg ctgctcgata ccgaacatcg ctcgatagtg ctgcaatcgg agataaacag
     2221 agtctcaggc gcacttcaac agatggagat gaattttctg atggacggca tcataatcaa
     2281 tctcgaggat atttcggaag aaccacagga aacttcctcg cctgagcaca aacaatacac
     2341 ctttgcaaag ctgaaccctg cccaaccctt tgatattccc attgaggttc cgaaagagag
     2401 ctttgactac tcattccgta tcttggatac cgcagtcatc gtcgaaccgg tcattacgcc
     2461 cggagtatct tactcgattc agctctatgt gacctcccat aaagtgatgt tacaccaact
     2521 caaagggttg agcccggtct tcgaaaagag acagtcttcc ggcaaatatc tctacaccgc
     2581 aggtattttc cacacttaca atgaagcgct ctctcagatc aacatcgtga agaaaagggg
     2641 cttcccttct gcaattctgg tggcctacct tgacggtaaa agcatctccg tacatgaggc
     2701 tagaaaagcg gagaagatgc ctgttgcggg gcctaaatat cgtatcgtac tggaaacctt
     2761 ttcagagggc atcccagatg ccattatggc catcataagg aataatacct caaaagaggt
     2821 aatcaggagc atctccgaag gaaaagtact ctacataata gctcccttcg atgaaaaaga
     2881 ggaggccgaa gcatttgcca ccatactaaa aggcaatggt gtggagggaa taacaacaga
     2941 aacaattaaa taattaaata ttatgggaac cggacttatt ataatgctaa tcgtattggg
     3001 tataatcctg atacttgtgg aaattcttct cataccggga tttgccgtca caggtgtact
     3061 cggtgtcgga tccctggtgc ttgccagcta cttcggattc acgcatcttg gaacccgggc
     3121 gggtattatc atcatcgtgg ttgaggtcat agtgctttcg gtattgctct actatgcact
     3181 ccaggccaaa acctggaaaa agctctctct ccacaccaat atcgatgcca aagtagatga
     3241 acatcccgag accaaaggtc ttacttccgg catggaaggt atctcaacca cccgtctcaa
     3301 ccctatggga agagctaaaa tcggaggatt tgatcttgaa gtgcgctcca ttgagggatt
     3361 aatcgatgaa cgtacggaga tcgtcataga ctccattgaa gaggagaaaa tatttgtaaa
     3421 aaagaaataa caatctaaaa tcaaaccatt atggatggaa ttacatcagt ttttatttgg
     3481 ataggggtcg ccatagtcgg cctgattatc cttttgtggt ttttcccgat cacaatgtgg
     3541 ttccaggcac ttatttcagg tgtcaggatt tcactgcttc aactaattct catgcgttgg
     3601 agaaaagttc ctcccggaac catcgtcatg gcgatgatta ccggtacaaa agcgggactt
     3661 acactcaatg ccaatgaact tgaagcccac tatctggcca aaggcaacgt accgaaagtg
     3721 gtcaatgctc tgatttctgc cgataaggca aatatagccc tgaacttcaa gatggcggct
     3781 gccatcgatc tggccggtcg cgatgttttt gaggcagtac aaatgtccgt aaaccccaag
     3841 gtgataaaca ctcctcccgt tacggcagtt gccaaagatg gtattcaact cattgtaaag
     3901 gctcgcgtaa ccgtacgagc caatatcaaa caacttgtcg gaggtgccgg tgaagagacc
     3961 gtactcgcac gtgtgggtga gggtatcgta tccagcatcg gttcatccga atcccacaaa
     4021 gaggtactcg agaaccccga ctccatctct aaagtggtac tcaacaaagg tcttgacgcg
     4081 ggtaccgcat ttgaaatcct ctctatcgac atcgcagaca tagatattgg aaaaaatatc
     4141 ggcgccgtac ttaagattga ccaggctaat gccgacaaaa acatcgctca ggcccgcgct
     4201 gaggagaaac gcgcaatggc tgttgccgtg gagcaggaaa tgaaggcaaa ggcacaggaa
     4261 gcgcgtgcaa gggttattga agctgaggca caggtacctc tggcgatagc tgaagccttc
     4321 cgcagcggca atctcggcat aatggattat tacaaattcc aaaacataaa ggccgacacc
     4381 gatatgcgcg aaagcatctc aaaaggaagc aaataggctt tggagaaata ttaacaaaaa
     4441 atgcaccggc cggtgcattt ttttattacc tttggaaaaa tactattacg atggactcct
     4501 ttatcaagaa actctattcg cgagtctctg tacttgccat tttcctgatt gtttctactc
     4561 tttcatcagc tcaaaactct ccggatggat ctttagaatg gtatttacct gacggggaac
     4621 accaattcaa cacctcgata ccgaccccag aacaagttct gggttatcag atcagtacct
     4681 atttcctcaa ttggggtgat gtatgcaaat atatggaacg acttgaggct gcctccgaca
     4741 gagtttccct tagaagattc ggtcgtacat acaactacag agagtttata cagatataca
     4801 tctcttccga aaaaaacatc tccaatcttg aaaaaatcag agaggagcac ctgctactct
     4861 gcgatcctac acgctcctcg ctgcttgaaa cggataaaat gccggtgatt gtaaatttga
     4921 tggcttctgt gcacggaaat gaaacctcgg gagttaatgc cgctatgctt gctgcatatt
     4981 ttttcgcagc atcagactct ccgcaaatca aggagattct cgaaaatacg atagtggtca
     5041 tcactccggg gctcaatcct gatggtatca gcagatttgc tcactggacc aactccacgg
     5101 caagcaaaaa caaaagagct gacattaatg gccccggaca gatggaacct tggcctagca
     5161 gccgcaccaa ccactattat gctgactgta accgagactg gctcagtgta caacatcctg
     5221 aagggatcaa tagtgtcacc atgtataaat ggtggatgcc gaatgtagtg ctggatatgc
     5281 atgaacaggg tactacttcg ggtttctttt ttagtcccgg agatcccaat agaacctata
     5341 gtggcatctc gcaggctaat caggatctta ccctcaaaat tgcgaagaat actgcaatcg
     5401 aacttgacaa aaaaggtctt ttatatttct ctaaagagag ttatgatgac ttttatattg
     5461 gtaaaggtgc ctcctatggt gatcttcagg gttctatttg cattcttcat gagcagggtg
     5521 cttcttacgg atatctacgc aataccccct ggggaattct gacatttcct gaaacgatac
     5581 gcacacaaac tcttgcagct atctccgtgg tcaaatcggg cttccaaatg agagaagaac
     5641 tgctcagtta tcagaaaaat tacttcatag atcagcttaa aaccgcccaa aaggacccga
     5701 ttgcaggagt aagatttgct gtcaaagggg acagggctcg cgaatatcat ctactggaga
     5761 tacttctgac tcacgacata gaggtctacc gcgacaataa ggagccgggg tatatagtcc
     5821 cgatagttca aaaccgctac tacactttca aggcaatgtg ggatcacatt acctcattta
     5881 aagatagtgt cttctacgat atatcaacat ggagtttccc ttcagcattc ggtatagaga
     5941 gcgaaaatct taaatccatt caagggcgac tttcggagag agtcactaag gcaatatttc
     6001 ccaaaggagg aataagcggc ggcataagcc acattgggta tgcattcagc aataatgagt
     6061 attatgctcc taagatggcc tcagagctac tcaaagaggg ctttttttta agggtggccg
     6121 gtaagagttt tacctcctta gaagataatc gggagtatac tttccctgcc ggtaccattt
     6181 tcatgcctgt tattggacag ccggcagaca aagaagctct ctatgagagg atttcggctc
     6241 tggcggagca gaccggagtg caggtacatt cttttaaaag tgccatgatg aaggattatg
     6301 atctaggcag tcccatttac tccagcctca aaaagcccga tgtggcaatc atcaccggcc
     6361 gaggactgag tgtttccaat attggagagg catggctctc catagaccgc aggtttgaat
     6421 ttgatcacac ggtaatcgac cattcacgca taacctcttc acgcgacctt tcgtcataca
     6481 acgtaatggt tttacttgga ggagtaccat cccctcttca acagggtgat ttttatgaga
     6541 ctctcggtag ttgggtaaga caggggggca cactgattct gggaggagac gctgctccta
     6601 tagcacagaa ggcaggactt tgtgaaattg taactaccac cggtaaaact atttccggag
     6661 tgattctgaa ttgtgaaata gacttagaat cccctctttt ctggggtttc agcgagaacg
     6721 ctttgcaagt attcaagcaa aaggcgaccg tttattcatg tgaaaagggt tctaccccta
     6781 tgaaatattc aaaggaccct tacatttcag gatacaccac ccctgaaatt attgataatt
     6841 tccgtggagg acaagctatc ataactcata aggtgggaaa agggcggtta attt
//
LOCUS       DAOSIE010000053         6864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_10034_length_6864_cov_3.852254, whole genome shotgun sequence.
ACCESSION   DAOSIE010000053 DAOSIE010000000
VERSION     DAOSIE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10034_length_6864_cov_3.852254"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PLI69_02695"
     CDS             <1..1336
                     /locus_tag="PLI69_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG41431.1"
                     /translation="WYLHKNAEAFNPDFEMLERDTLRSSIPDSVYIERLNRLNSFIPI
                     QFNHYVRNNIILYTEKMPKVTANIISLSTYYLPIIEGIFDMYDLPKELKAMAMIESAF
                     NTKAVSRAKARGMWQFMHYTARLYDLDMTSFVDERYDPYKSCHAAARYLRDSYNIFGD
                     WSLAIASYNCGAGNVLKAIRRSGGKTDFWDIYHYLPRETRGYVPIFFAALYTLKYYPE
                     HGIVPQQCALPAHVDTIHVNKMLHFQQISDVINIPMEDLRQLNPQYLHDIIPGKEKTY
                     ILRLPHNYTMQFVSNEESIYSYKDTLFFSDVAIKKVKEKGGTEGSMTTHTVRSGETLG
                     AIARKYRTTVTNIKRWNNLRSDVIRVGQKLRIGGSAASSRSSSSTASSDSAAVADTKD
                     GYLTYTVKKNDTLTAIASKFSGVTVNSLLQLNGFTQSTKIYPGMVIKIKKAQ"
     gene            complement(1297..3198)
                     /locus_tag="PLI69_02700"
     CDS             complement(1297..3198)
                     /locus_tag="PLI69_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41432.1"
                     /translation="MRREIVGFERVLFAIAIAVAIFFCTTSDCKGQHRLPHQLISMIE
                     DIASSGGEEAALELQLKYEELLYSPLEINMLTRRQMEECGLLTPFQIESILDHRREYG
                     DILSLSELSLVDGFGQEAIELVRYFFRFSSLQEIASPLVEESFGHRFSTKVRKKRDSE
                     GISLTSKYKIEYGKRISAGLTIDSDAGEKLTPYYLPDFTSLHLAYSPPGIPDSDKKLR
                     LERVVIGDYVARFGQGLALWKAFPVSFFGNPSSLIKRGGAIAPYTSTDESNFLRGAGI
                     TFGSREIECSLFASYNGVDARVVGDSCYTSIIRDGLHTTDTERATRNSMHEYLFGGHL
                     SYDFNRMQLGVTALCYGYDKCNARRVQEYNRYQIYNGLWGNMALDLYSSWRSFRFFAE
                     AALDFRPAAALLCGLLWNPDYKLEMALLFRAYGKGYTATHAAAYSTLSNCSNQYGATA
                     SLKYMPFRNWTFSLSAEYSAYPWSRYRIEGSSRDSRARFDIIRESRNGSKIQIRLDWR
                     HKSESGSTLKGRLHTLFVLSQCWSLSARIEGGVKGFATYGELGFRSRDRRWDLCARGT
                     YYNTEDWKSRVYIYERGVPQSFSVENYYGKGWSWYFLMKWSPARFADLWLKYSKSYCA
                     FFILITIPG"
     gene            complement(3240..3752)
                     /gene="purE"
                     /locus_tag="PLI69_02705"
     CDS             complement(3240..3752)
                     /gene="purE"
                     /locus_tag="PLI69_02705"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOG41433.1"
                     /translation="MRAQVSIIMGSTSDMPVMKQAAEFLEMMEVPFEINALSAHRVPH
                     RVMEFAEGAAARGIKVIIAGAGGAAHLPGVIAAYTPLPVIGVPIKSSNSIDGWDSLLS
                     ILQMPSGIPVATVALDGARNAAILAVQMLALSDVALMEKVKAFKADLASRISKANDEL
                     AEIKYKFKTN"
     gene            3823..4371
                     /gene="hpt"
                     /locus_tag="PLI69_02710"
     CDS             3823..4371
                     /gene="hpt"
                     /locus_tag="PLI69_02710"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOG41434.1"
                     /translation="MERIKLHDKYFVPYIRYEEISKAIDVVAEKINKDFRGREKPPIL
                     LCVLNGSIMFTGELMKRITFPCEITSIRLASYEGTLSSGKTKTILGLTADIKGRDLII
                     VEDIVDTGTTIYDIHKTLTIAGANDIKICTLLLKPEVYNKSLRLDYVAMEVDNRFLVG
                     FGLDYNQLGRNSKDIYILDKSS"
     gene            4394..4972
                     /locus_tag="PLI69_02715"
     CDS             4394..4972
                     /locus_tag="PLI69_02715"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOG41435.1"
                     /translation="MKHFIIIGPPGAGKGTQAKLIVEKHNLRHISTGDLLRAEIKADT
                     KLGRKIKAIIDDGNFVPDEMVLKMVKSVITQYPDVSGFIFDGFPRTTIQASDFDSHLK
                     SLGMDVNGVISIIISDDIVKDRIHHRAMIEDRKDDMNDEIIQNRIATYHAKTEPLIDY
                     YKNQGKYHEIDGNGTIEEIFTTISTLIERLSK"
     gene            4977..5996
                     /gene="obgE"
                     /locus_tag="PLI69_02720"
     CDS             4977..5996
                     /gene="obgE"
                     /locus_tag="PLI69_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOG41436.1"
                     /translation="MRNQGSNFIDYVRIFCKSGHGGKGSTHLRREKYIPKGGPDGGDG
                     GRGGHIILKGDKQLWTLIHLKYRKHIKAENGVDGSGQLRTGADGKDIILKVPLGTVAK
                     DGETGEVLFEIVDDGQETILAQGGRGGLGNNNFKSPTNQTPRYAQPGEEGVEGWFVLE
                     LKILADVGLVGFPNAGKSTLLSVVSAAKPKIADYAFTTLEPNLGIVNYDDDKSFVMAD
                     IPGIIEGAHEGRGLGLRFLRHIERNSMLLFMIPADSKDISGEYDILLNELKQYNPELL
                     DKERLLAVSKSDMLDDELKKEIKKHLPKKIPYLFISSLTGEGIPQLKDMLWKTLNSDG
                     EIDRT"
     gene            5974..6531
                     /locus_tag="PLI69_02725"
     CDS             5974..6531
                     /locus_tag="PLI69_02725"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOG41437.1"
                     /translation="MEKLIELDMKLFTLLNGWHSDFFDPIMVFLSKVWVWVPLYLAVM
                     VYLFVTKKWKVALLAVVTIVLAYLASEHISVFIKESVARFRPCEEPLLADSVRMLEPH
                     GSLYGFVSGHACNTFCFAALSSLIIRKRLYTILIVIWASLISYSRIYVGKHYPADIIG
                     GALLGLLIAYLFWLIYKTVYKKLCS"
     gene            6522..>6864
                     /locus_tag="PLI69_02730"
     CDS             6522..>6864
                     /locus_tag="PLI69_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOG41438.1"
                     /translation="MLLIYDTNTDPHWNLAAEEYLLENFSESIFRLWRNADSIIIGRN
                     QNAYAEIDTEWVSSKGIPVVRRLSGGGAVFHDLGNVNFSFFESGISRQESNEMFRRFT
                     GPILDALHSLGV"
BASE COUNT         1996 a         1584 c         1523 g         1761 t
ORIGIN      
        1 gtggtatttg cacaaaaatg ccgaggcatt caaccctgat tttgaaatgc tggaaaggga
       61 taccctcagg tcatccattc ctgactctgt ttatattgaa aggctaaaca gattgaattc
      121 atttattccc attcaattca accactacgt aaggaacaat atcattcttt acacggaaaa
      181 aatgcctaag gtgactgcca acatcatctc tctttcgacc tattatctcc ctataatcga
      241 ggggattttt gatatgtatg acctacccaa ggagctcaaa gccatggcaa tgatcgagtc
      301 cgcatttaac acaaaagcag tctcgagggc taaggcgagg ggaatgtggc aattcatgca
      361 ctataccgca agactttacg atctggatat gacatcgttt gtggatgaaa ggtatgatcc
      421 atataaatcc tgtcatgcgg cagcaagata cctcagggac tcatacaata ttttcggaga
      481 ctggtctctt gccatagcat catataattg tggcgccggc aatgtgctga aagccatacg
      541 ccgctcaggt ggaaagactg acttctggga tatataccat tatctgccca gagagacaag
      601 aggatatgtt ccaattttct ttgcagctct atatactttg aaatattatc ctgaacacgg
      661 tatagtgcct cagcaatgtg cacttccggc ccatgtggac acaatacacg tcaacaagat
      721 gcttcatttc caacaaattt ccgatgtgat taacatccct atggaggatc tgcgccagct
      781 caacccccaa tacctccacg atattattcc cggaaaggaa aaaacctata tactaagact
      841 tccgcataac tacacgatgc aattcgtaag caatgaggag tcgatttact cttataaaga
      901 tacgctcttc ttcagcgatg tggctatcaa aaaggttaag gagaaaggtg gtacggaggg
      961 gagcatgacc acacataccg ttaggtcggg tgaaacattg ggtgccatag cccgaaaata
     1021 tcgcactacg gttaccaata taaagagatg gaataatctc aggagcgatg tgatccgtgt
     1081 gggtcaaaaa ttacgcatcg gaggcagcgc cgcatcatca aggtcaagca gttcgacggc
     1141 atcttccgat agtgcggccg tagccgatac taaagatgga tatttaacct atacggttaa
     1201 gaaaaatgat actttaaccg ctattgcgag caaattctcc ggtgtgacgg taaactcatt
     1261 actccagctg aatggattta cacaatccac aaagatttat ccggggatgg taatcaagat
     1321 aaagaaggca caatagctct tcgaatattt aagccataag tctgcaaacc tcgcaggcga
     1381 ccatttcata aggaaatacc agctccaacc cttgccgtag taattctcca cagaaaatga
     1441 ttggggtact cccctttcat atatgtagac tcgcgacttc caatcttcgg tattgtaata
     1501 ggtgcctctg gcacatagat cccatctgcg atctctcgaa cgaaacccaa gctccccata
     1561 agtagcaaag cctttcactc ctccttcgat ccgcgcagaa agagaccagc attgggaaag
     1621 tacaaataac gtatgtaatc gtcctttaag ggtggatccg gattcgctct tgtggcgcca
     1681 gtcaagtcgt atctgaatct ttgaaccatt gcgggactcc cttattatgt cgaatcgtgc
     1741 tctggagtcc cgggatgagc cttcaatcct atatcgggac caaggatagg cgctatactc
     1801 cgcagacaga gagaatgtcc agtttctaaa aggcatatat ttgagactcg cggtagctcc
     1861 atactggttt gagcaattgg agagagtgga gtaggcagcg gcgtgagtag cggtgtagcc
     1921 cttaccgtaa gctcgaaaca gaagggccat ctccagcttg taatcgggat tccataagag
     1981 gccgcacagc agcgctgctg ccggtcggaa gtccagggca gcctctgcga agaaacggaa
     2041 actacgccac gacgagtaga gatccagtgc catattaccc cagagaccat tatagatctg
     2101 atatcggttg tactcttgta cgcgacgggc gttgcacttg tcatatccgt agcagagtgc
     2161 ggttactcct aactgcatac ggttaaagtc gtaagataag tgtccgccga atagatattc
     2221 atgcatgctg ttgcgtgtgg cacgttcagt atccgtagta tgaaggccat ctctgattat
     2281 tgaagtatag caactgtctc caactaccct cgcatctact ccgttgtaag aagcaaaaag
     2341 tgagcattca atctcccttg aaccgaaggt tatccctgct ccccgcagga aattcgactc
     2401 atcagtagag gtataggggg caatggcgcc accacgttta atcagggatg agggattccc
     2461 gaaaaaagag accggaaagg ccttccacaa tgcaagccct tgtccgaatc tggcaacata
     2521 atctcctatc accactctct ccaatcttaa ctttttgtca ctatccggta taccgggcgg
     2581 actatatgcc aggtgcagag aggtaaagtc gggcagatag taaggagtaa gtttctctcc
     2641 cgcatcactg tcgatagtca gaccggcgct aatacgtttt ccatattcaa ttttgtattt
     2701 tgaggtcaat gaaatacctt cagagtctct tttttttcga actttagtag agaaacgatg
     2761 tccgaaactc tcctccacta agggagaagc aatctcttgg agtgaggaaa atctgaagaa
     2821 atacctcacc aattcaatag cctcctgacc gaaaccatct accagcgata gctccgaaag
     2881 tgaaagtata tccccatact cccttctatg atccaaaatg ctttcaatct gaaacggagt
     2941 aagtaagcca cactcctcca tctgtcgcct ggtaagcata ttgatctcca atggcgaata
     3001 gagcaactcc tcatatttga gctgaagctc aagagccgcc tcctcccctc cggaagaggc
     3061 aatatcttca atcattgaaa taagttgatg aggcagcctg tgctgccctt tacaatctga
     3121 tgttgtacaa aaaaagattg ccaccgctat ggcgatggca aacaggactc tttcaaaccc
     3181 cacaatctct cgcctcatat tccccgaaaa gttaaactat gataatcaaa aatgcatagt
     3241 caatttgttt tgaacttgta cttgatttca gccaattcat cgttggcctt ggatatcctg
     3301 gatgccaaat cagccttaaa agccttaact ttctccatca atgccacatc ggagagtgcc
     3361 agcatctgta ctgcgagaat tgcggcattc ctcgctccat cgagggcaac cgtagctacc
     3421 ggtatacccg aaggcatttg tagaatggaa aggagtgaat cccacccatc gatggaattt
     3481 gaagatttta tcggaacgcc tataaccggc agaggagtgt atgcagcaat gactcccgga
     3541 agatgggctg ctccaccggc acccgcaatt atgaccttga ttccacgggc cgcagctcct
     3601 tccgcaaact ccataaccct gtggggcact ctatgtgccg acagagcatt tatttcgaaa
     3661 ggtacttcca tcatttccag aaactccgca gcctgtttca tcacaggcat atcggaagta
     3721 ctccccatta taatacttac ttgcgctctc atattgtctc attttatatc atgtcccaaa
     3781 aataatgatt aaatttgcca ctgcaaaata attatcttac agatggaaag aatcaaacta
     3841 cacgacaaat attttgtgcc atacatccgt tacgaggaga tttcaaaagc cattgatgtg
     3901 gtggctgaaa aaataaacaa ggattttcgc ggacgggaaa agcctccaat cctgctctgc
     3961 gtactcaacg gatcgataat gtttaccggt gagctgatga aaaggatcac cttcccctgt
     4021 gagattacca gcatcaggct ggcctcctac gaaggcacgc tctcttcggg caaaacaaag
     4081 acaattctcg gacttaccgc tgacataaaa ggtagagacc tgatcatcgt ggaagatata
     4141 gttgatacag gcactaccat ttacgacata cacaaaacac ttaccatagc cggagccaat
     4201 gacatcaaga tatgtacact cctgctcaag cccgaagtct ataacaaatc tctgcgtctc
     4261 gactatgtgg caatggaggt tgacaatcgt ttcctcgtag ggtttggtct ggactataac
     4321 cagctgggac gtaatagcaa agacatatac atcctcgaca aatcatctta acattgcata
     4381 agacacatat atcatgaagc actttataat tattggccca cccggtgccg gaaaaggtac
     4441 tcaagcgaag cttattgttg aaaaacataa tttaagacac atatccaccg gagaccttct
     4501 tcgggcagag ataaaagccg atactaaact ggggcgtaag atcaaggcca ttatagacga
     4561 cggcaacttc gttccggacg aaatggttct caagatggta aagagtgtga ttactcaata
     4621 tccggatgtg tcaggcttta tttttgatgg gtttccccgc actaccatac aagcctccga
     4681 ttttgactct catctaaaaa gtctcggaat ggatgttaac ggtgtgattt caatcataat
     4741 cagtgatgac attgtaaaag atagaattca tcacagagca atgattgaag atcgcaagga
     4801 tgacatgaac gatgagataa tacaaaaccg tatagccacc taccacgcta agacggagcc
     4861 attaatagat tattacaaaa atcaaggcaa gtaccacgaa attgacggca acggtacgat
     4921 tgaggaaatt ttcaccacaa tatctaccct gattgagagg ctttccaaat agaataatga
     4981 gaaatcaagg ttccaacttc atagattatg tccgcatatt ttgtaaatcg ggtcacggag
     5041 gaaagggttc cactcacctc cgcagggaaa aatatatccc caaaggaggt cccgacggag
     5101 gagatggagg aaggggcggc catataatat taaagggtga caagcagctc tggactctca
     5161 ttcacctaaa atatcgcaaa catatcaagg cagaaaacgg agtagacggt agcggacaac
     5221 tacggacagg agcagatggt aaggatatta tcctcaaagt acccttggga actgtagcca
     5281 aagatggtga aacgggtgaa gtgctctttg aaattgttga tgacggccag gagacaattc
     5341 ttgcgcaagg cggccgtgga ggtctgggta acaacaattt caaaagtcct accaatcaga
     5401 cgccccgtta tgcgcaaccc ggggaagagg gtgttgaagg ctggtttgtc ctcgaactga
     5461 aaatattggc tgatgtggga ctggtgggat ttccaaatgc tggtaagtca actctactct
     5521 ctgtagtgtc tgctgccaag cccaaaatag cggactatgc atttaccaca ctcgaaccta
     5581 atcttggcat agtaaattat gacgatgaca agtcctttgt aatggcagat attccaggta
     5641 tcatagaagg tgcccatgaa ggaagaggac ttgggcttag atttctaaga cacattgaac
     5701 gcaactcaat gctgctcttt atgataccgg cagatagcaa ggatatctcc ggagagtatg
     5761 atattctact caatgagctg aaacaataca atccggaact actggacaag gagcgtcttc
     5821 tggctgtcag caagtccgat atgctggatg acgaacttaa aaaggagatc aaaaagcatc
     5881 tccccaaaaa gatcccatat ctctttatct cttctctaac aggtgaaggg atacctcagc
     5941 ttaaggatat gctctggaag accctgaaca gcgatggaga aattgatcga acttgatatg
     6001 aaattgttca cccttctaaa cgggtggcat tcggatttct ttgatccgat tatggttttt
     6061 ctctcaaaag tatgggtgtg ggtaccgctc tatctggcgg ttatggtcta tcttttcgtt
     6121 accaaaaaat ggaaagtggc gctgctggca gtggtgacca ttgtgctggc atacctcgcc
     6181 agcgagcaca tatcggtatt catcaaagag agtgtggccc gctttaggcc ttgtgaagag
     6241 cctttgctcg cggatagtgt caggatgctg gagccccacg ggtccctcta cggatttgta
     6301 tccggccacg cgtgcaatac cttttgcttt gcagctctct cctcgttgat tatacgcaaa
     6361 agactctata ctatccttat cgttatctgg gcatccttga tatcatacag caggatttat
     6421 gtaggtaagc actatcctgc cgacattatt ggcggtgcac tcctgggcct gttgattgcg
     6481 tatctctttt ggctcatcta taagaccgtt tataaaaaat tatgctcctg atctacgata
     6541 ccaataccga ccctcactgg aacctcgccg ctgaggagta cctccttgag aattttagtg
     6601 agagtatttt tcgcctatgg cgaaatgcag actcaattat cattggtcgc aatcaaaatg
     6661 catacgcaga gattgacacg gaatgggttt cttcaaaagg tattccggtg gtacgcagat
     6721 tgagtggggg cggtgcggtt tttcacgatc tgggaaatgt aaatttttcc tttttcgaga
     6781 gcggaatttc ccgtcaggag agcaatgaga tgtttcgtag gtttaccggc cctattttag
     6841 atgcacttca ttcactcgga gtgg
//
LOCUS       DAOSIE010000054         6829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_10109_length_6829_cov_4.169619, whole genome shotgun sequence.
ACCESSION   DAOSIE010000054 DAOSIE010000000
VERSION     DAOSIE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10109_length_6829_cov_4.169619"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2577
                     /gene="alaS"
                     /locus_tag="PLI69_02735"
     CDS             <1..2577
                     /gene="alaS"
                     /locus_tag="PLI69_02735"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOG41439.1"
                     /translation="SKGHTIVPSAPMVVKNDPTLMFTNAGMNQFKDWFLGNTPVKHPR
                     VANTQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFKKDAINWAWELLTEVY
                     KIDKDRLYATIFEGYAPEGLEMDKEAHDLWLKLLPEERVILGNKKDNFWEMGDTGPCG
                     PCSEIHIDLRSDEERAKIPGREMVNRGHHLVIEVWNLVFIQYNRKSNGSLEPLAAKHV
                     DTGMGLERLCMVLQNKQSNYDTDVFTSMIEAISRVSGVKYESDSEVGVAMRVIADHIR
                     AICFSIADGQLPSNVKAGYVIRRILRRAVRYGYTFLGMHEPFMCKLVPQLVSDMGEAF
                     PELVKQQDLIVNVIREEEDAFLRTLDKGIRLMDSILEKSAKSKQISGVDAFTLYDTFG
                     FPIDLSELIAREKGYTIDMKGFEIELGKQKERARNATAIEEGDWYEIAPFTSTGFVGY
                     DTLEAEVHIMKYRTVKAKNKEMFQLVFDKTPFYAESGGQVGDSGTIESASGELITILN
                     TIKENELPIHIADRIPQNIKESFIAKVSEERRIETANNHTATHLLHHALRHVLGNHIE
                     QKGSSVSATSFRFDFSHYEKVSREHLREVEKLVNSLIRKNIAREEFRNIRVEKAHEMG
                     AIALFGEKYGDEVRVIRFGDSTELCGGTHAPSTGSLGYFRILSETAIAAGVRRIEATT
                     GKHAEATVYEMDDNIIALGALFNNNPNLLGSVTKLISDNETYKKELEEVEKERAIALK
                     KRLIEESKVVDGIRLITLRGGSFNQDMVREVAFMLHKELTSAVFIAAIPSSDGKANLT
                     LMYTDDLVKKGANASNDIKIAAKLIQGGGGGQNFLATAGGKNVEGLDEAADKLEEIAL
                     SR"
     gene            2577..3923
                     /locus_tag="PLI69_02740"
                     /pseudo
     CDS             2577..3923
                     /locus_tag="PLI69_02740"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
     gene            4077..5009
                     /gene="fmt"
                     /locus_tag="PLI69_02745"
     CDS             4077..5009
                     /gene="fmt"
                     /locus_tag="PLI69_02745"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOG41440.1"
                     /translation="MGTPEFAVAPLESLLAEGFDVVAVVTAPDKQSGRGLKINESAVK
                     KCAVGHGIPVFQPERFKDPEFLEQLASFNANLFVVVAFRMLPVEVWSMPSLGTFNLHA
                     SLLPQYRGAAPINWALINGERITGVTTFFINEEIDTGKILFQESYSIEEYDNAGTLHD
                     KLMEMGAALVVKTASAIRDRTVRPVAQDTQYMALRPAPKLSRGLARIDWSRDARDIVN
                     LIRGLSPYPCAHTTFVFEEQQLGVKIFEAFVREKDQENEDWGTITSDGSSWLEIKCGI
                     GSVRILNLQIAGKRRLDIGEFLKGFREPEKYRAK"
     gene            5019..5642
                     /locus_tag="PLI69_02750"
     CDS             5019..5642
                     /locus_tag="PLI69_02750"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOG41441.1"
                     /translation="MKRIVILADGTYPSSEKTLKILRTAEVLICCDGAAEKAISRGVI
                     PDFIAGDMDSMPIELQREYSDIIRKSDCQDTNDLTKAVELAKSFNPLQVTILGATGRR
                     EDHALGNISLLAEYAQECPFPVDMVTDHGRFFTTKDSTVFDAAPGTAISFFTFDQSLS
                     IKSTGLEYPLDEVIFDSLWKASLNIVSESKVSLKFSHPAIVLIFEAE"
     gene            5785..6168
                     /locus_tag="PLI69_02755"
     CDS             5785..6168
                     /locus_tag="PLI69_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41442.1"
                     /translation="MRKIIAYSTILILSLSCKSQQFNVVSLCGTFEGVEGGENPLSTY
                     VVLELNMDNTCSLKKTFDLSKIECRGEWTINDDVIEIRCNNNPVLSDVEKALQGGSYI
                     EGTLKIIVLSKNKLKLGNTVLKRKK"
     gene            6224..6700
                     /locus_tag="PLI69_02760"
     CDS             6224..6700
                     /locus_tag="PLI69_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41443.1"
                     /translation="MKKIFAIITILFMASCVYSQEAVYVEGKGYEGYVFPKEHSIWDF
                     PPEQNRYTPNLEDIALAEKILRDSIGTDYVKSNQQAYRKPPINNKTLKKYVRQYVGYV
                     TEECEIIIHIYLNKGIETDKNKLSEEIIAIFGGGSNHWHIKINISTKELFDMQLNK"
BASE COUNT         2035 a         1427 c         1649 g         1718 t
ORIGIN      
        1 tctaaaggcc atactattgt gccctcggca ccaatggtgg tcaaaaatga ccctacgctt
       61 atgtttacca atgcgggcat gaatcagttc aaagactggt ttctcggcaa tacccccgta
      121 aaacatccca gagtggctaa tacgcaaaaa tgtctcaggg taagcggcaa gcacaatgac
      181 ctcgaggagg tcggccacga cacctatcac catacgatgt ttgagatgct cggcaactgg
      241 agtttcggtg actatttcaa aaaagatgcc ataaactggg cttgggaact tctgactgaa
      301 gtttacaaaa tcgataagga ccggctatat gccacaatat ttgagggtta cgcaccggaa
      361 ggcctcgaaa tggacaaaga ggcgcatgat ttatggctca agctccttcc ggaagagaga
      421 gttattctcg gcaacaaaaa ggataacttc tgggaaatgg gtgatacagg tccctgcggc
      481 ccttgcagcg agatccacat agacctccgc agcgatgagg agagagcaaa aatccctggc
      541 agagaaatgg taaaccgggg acatcatctg gtaatagagg tatggaatct cgtcttcata
      601 cagtacaacc gcaaatccaa cggctctctg gaacctctcg cagccaaaca cgtagatacg
      661 ggaatgggac ttgagcgcct ctgtatggta ttacagaaca aacagagtaa ctacgatacc
      721 gatgtattca cctccatgat tgaggccata tcgagggtca gcggggtaaa atatgagagc
      781 gatagtgaag tgggagtagc aatgagggtt atcgcagacc acataagggc catctgtttc
      841 tctatcgctg acggacagtt gccctctaat gtcaaggcag gctatgttat caggcgtatt
      901 cttcgccgtg ccgtaagata cggctatact ttcctcggca tgcacgagcc atttatgtgc
      961 aagctcgtac cccaacttgt aagtgacatg ggtgaagcct tccccgaact ggtaaagcag
     1021 caagatctga tcgtaaatgt gatacgagag gaagaagatg catttttacg cactctcgac
     1081 aagggcattc gccttatgga ttccatactg gaaaaatctg cgaagagcaa acagatctct
     1141 ggggttgacg cttttactct atatgataca tttggatttc caattgacct gagtgaactt
     1201 attgccagag agaagggcta cacaatagat atgaagggct tcgaaatcga acttggcaaa
     1261 cagaaggagc gtgcgagaaa tgctactgcc atcgaagagg gtgactggta tgagattgcc
     1321 cccttcactt cgaccgggtt tgtaggttac gacaccctcg aggcagaagt acacattatg
     1381 aaataccgta ctgtcaaagc caaaaacaaa gagatgttcc agttggtatt tgacaaaacc
     1441 cctttctatg cagaaagcgg aggacaggtg ggcgatagcg gtacaattga gtcggcgagc
     1501 ggtgagctta tcaccatttt gaataccatc aaagagaatg agctccccat acatatcgca
     1561 gataggattc ctcaaaacat caaggagagt tttattgcaa aagtgagtga agaacgcaga
     1621 attgaaacgg caaacaatca cacagctacc catttgctgc atcacgctct gcgacacgta
     1681 ttgggcaacc atatcgaaca aaagggctct tcagtcagcg caacttcatt ccgttttgac
     1741 ttttctcact acgagaaagt ctcccgagag catctgagag aggtggaaaa actcgtcaat
     1801 agccttatcc gtaaaaatat cgcgagagag gagttcagaa acataagggt tgaaaaggca
     1861 catgaaatgg gcgctatagc acttttcgga gaaaaatatg gggatgaggt acgcgtgata
     1921 aggtttgggg actctacgga actttgcggc ggcacccatg ctccctctac cggctccctt
     1981 ggctatttca ggatactgag cgaaactgcc attgctgcgg gagtcaggag aatcgaggct
     2041 acgacaggca agcatgccga ggcgacggta tatgagatgg atgataatat tatcgcactg
     2101 ggagcgctct tcaataacaa tccaaaccta ctcggttcag ttacaaaact tatctcagat
     2161 aacgagactt ataaaaagga gttggaagag gtggagaaag agcgtgccat agcacttaag
     2221 aagagactta tcgaagagag caaggtagtc gatggcatac gtctgataac cctcagaggt
     2281 ggtagcttca atcaggatat ggtcagagaa gtggcattta tgctccacaa ggaactaacc
     2341 tcggcagtat tcatcgctgc aataccctca agtgacggca aggccaatct tacattgatg
     2401 tacaccgatg atcttgtaaa aaagggagcc aatgcctcaa acgacatcaa gatagctgca
     2461 aagctgatcc aggggggcgg aggcggacaa aacttccttg caacagccgg tggcaagaat
     2521 gttgaggggc ttgatgaggc agcagataaa ttggaggaga ttgcactaag cagataatga
     2581 aaataattga ccggttccta ataaagaggt ttataggccc ctttttcctt acctttttca
     2641 tcgtgatatt cgtactctcg atgcagttcc tctggctcta tatagatgaa ctggtaggca
     2701 agggacttgg aatcggtgtg attttggaat ttctgggctg ggcatcggct acgctgattc
     2761 cgatggcgct acctttagcc accctactgg cctccattat gtcactcgga ggtatgggag
     2821 agcataacga attgctcgcc atgaaggccg cgggtatctc tctcggtaga atattgatgc
     2881 cggtgatggt gctttcggga ttgatatccg tcggggcatt ttttgctgcc aacgaccttg
     2941 tccccgtctc gtacaacaag atttacaccc ttcgcaacga tattctccat acaaaagatg
     3001 agattaagat acccaaagga atcttctacg acggtataga caactatata ctccgcatcg
     3061 aggagcgcaa cgacgagacc ggaatgatgt atgacatcat aatatatgac catacactcc
     3121 atgacggcaa caacaacata actattgccg attccggcaa aatggttgtc acttccgaca
     3181 aacaacatct gatatttgac ctctataacg gtatgtcata taaggaagag aataagatga
     3241 cctatagaga tacatccata accctcagta agcttcaatt tgaagagcaa caattgattg
     3301 tcgctttgga agattatacc ttcacaagat ccacggacga ccgtttcagc gatgaagtga
     3361 tgtctaaaga tctgaagcag ctcaggtatg attgggattc gatttcggtg gtctttgccg
     3421 aattgcaaaa aacgcagcaa aatagatttt tgtcagggtc gggaatgaat tatctccatc
     3481 agctggatac cacaataaaa aggaatttca caggatggct ggatattgat tctctttacg
     3541 cgtgggttgc tgccaatgac cccgtccaga aggagaagga ggcttgtcgt caggcgatgg
     3601 acagacttac cctcgctatg gatctggtgg gcaatttttc cagggagaac taccgctatg
     3661 tagatccgct caagaggatt gacatagaga gttaccgaaa gtttacccta tctctcgcct
     3721 gcttgctgtt tttcttcatc ggagcgccgc tcggagctat tattcgtaag ggaggccttg
     3781 gtactcccgt cattatttcg atattcttct atctggtata ctatatcatt gatatttcgg
     3841 gtaaaaaact tgcgaaagat ggagcgatga cgccatttgc aggtactata atttcagctg
     3901 tagtattgtt gccgataggc atttttctta ccagacacgc aatcagggat tccagcctct
     3961 tcaatataga ttcttacaag gtatttttca agaatatatg ggattatata gtcaagattt
     4021 accacaaggt acgcaatttc ttccgcaagg atagaggtcg catccgcatc gtctatatgg
     4081 gcactcccga atttgcggta gcaccgcttg agagtcttct cgcagagggg tttgatgtag
     4141 tggcagtagt tactgctccc gataagcaga gcgggcgcgg acttaaaata aatgagagcg
     4201 ccgtcaaaaa gtgcgcagta gggcatggta taccggtatt tcagcctgag aggttcaagg
     4261 accccgagtt tctggagcag ctcgcctctt tcaacgccaa tctctttgta gtggtagcct
     4321 tccgaatgct gcccgtagag gtctggtcga tgccttctct gggtactttc aacctgcacg
     4381 cctccctcct gccgcaatac cgcggtgcgg ctcccatcaa ctgggcgctg atcaacggag
     4441 aacgcatcac cggagtgaca actttcttta taaatgagga gattgacacc ggcaaaatac
     4501 tctttcagga gagctactct atcgaggaat atgataatgc cggtaccctg cacgacaaac
     4561 ttatggagat gggagccgct ctggtagtga agacagcaag cgccatcagg gaccgcactg
     4621 tacgtccggt ggcgcaggat acccaataca tggcactcag acccgcaccc aaactcagcc
     4681 ggggacttgc gcgcatcgac tggagcaggg atgcccgcga tattgtaaac ctcatccgag
     4741 gactcagccc ctacccctgc gctcatacga cctttgtttt tgaggagcag cagctaggag
     4801 tcaagatatt cgaggccttt gtgagggaga aggatcagga gaatgaggat tggggaacta
     4861 ttacctccga tggcagcagc tggctcgaga ttaagtgcgg tatcggatcg gtgcgtattt
     4921 taaatcttca gattgccggc aaaaggcggc tcgatatagg agagtttctt aaaggattcc
     4981 gtgaaccgga aaaatatagg gcaaaataag ttgcagctat gaagagaatt gtaatacttg
     5041 cagacgggac ctatccatct tcagagaaga ctctgaaaat actccgcact gcggaagtcc
     5101 tcatctgctg cgacggtgcc gcagaaaagg ccatctcgag gggagtaatt ccggatttca
     5161 tcgccggtga tatggattcg atgcctatag agttgcagag agaatactcc gatatcatca
     5221 gaaaatcgga ttgccaggat actaacgacc ttaccaaggc tgtggagctg gcaaagagct
     5281 tcaacccctt acaggtgacc attctcgggg ctacgggccg aagggaggat catgctctgg
     5341 gcaatatttc gctcctggct gaatatgcgc aggagtgccc tttccctgtg gatatggtca
     5401 cagatcacgg ccgatttttt acgaccaagg atagcaccgt ttttgatgca gctcccggca
     5461 ccgccatttc attttttacc ttcgaccaaa gtctgagcat aaaatcgacg gggctcgaat
     5521 accccctcga tgaagtgatt tttgattccc tctggaaagc ctctctcaac atcgtatcgg
     5581 agagcaaggt ttcgctcaaa ttttcccatc ccgccattgt tctgattttt gaagcggagt
     5641 agctaaccct tagggtcttc aaataatttt gcgggaataa aaattattga ttaattttat
     5701 ttcataatat tctgacatta agaatattat gaaatgggtg gtgtcacaaa tacagaatcc
     5761 caataaatat caattaaacg agagatgaga aaaattatag catattcaac aattttaata
     5821 ctatctctat cttgtaaaag ccagcaattt aatgttgttt ctttgtgcgg tacatttgaa
     5881 ggagtagagg gcggggaaaa tccattgtct acttacgtgg tgttggaatt gaatatggat
     5941 aatacttgtt cgttgaaaaa aacttttgat ttgtcaaaga ttgaatgtcg aggcgaatgg
     6001 actataaatg atgatgtaat agagataagg tgtaataata atcctgtttt gagcgatgtt
     6061 gaaaaagcat tgcagggagg aagttatatt gaaggaactc ttaaaataat agttttgagt
     6121 aaaaacaaat taaaattggg caataccgtt ttgaagcgga agaaataggc agttaaagcc
     6181 ccgcagtttt gcggtaataa aaacattaat taccgttgga gcaatgaaaa agatatttgc
     6241 aattataaca atcttgttta tggccagttg cgtttattcg caggaagcgg tatatgtaga
     6301 ggggaaagga tatgagggtt atgtttttcc aaaggaacat tcgatttggg attttccgcc
     6361 tgaacaaaat cgctatacgc caaatttaga ggatattgcg ctagcggaaa aaattttgcg
     6421 agacagtatc ggaaccgatt atgtgaaatc aaatcaacaa gcatatagga aaccgcccat
     6481 aaataataag acgcttaaaa aatatgtgcg tcaatatgtc ggatatgtaa cggaagagtg
     6541 cgaaattatc attcatattt acttaaacaa aggtatcgaa acggataaaa acaaattgtc
     6601 ggaagagata atagcaattt ttggcggagg aagcaaccat tggcatatca aaataaatat
     6661 atcaacaaag gaactatttg atatgcaact caacaagtag caaatatatt aacaaataga
     6721 gcgttaaaag atgaagagga aaataaaaga taataacttt ataacacttc ttgggatcaa
     6781 aataaatggt ggagtattag ttgcaataat aattttcgca ctttttttc
//
LOCUS       DAOSIE010000055         6829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_10111_length_6829_cov_3.233540, whole genome shotgun sequence.
ACCESSION   DAOSIE010000055 DAOSIE010000000
VERSION     DAOSIE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10111_length_6829_cov_3.233540"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="PLI69_02765"
     CDS             complement(<1..1314)
                     /locus_tag="PLI69_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG41444.1"
                     /translation="MTEQQIQAKKKNIHRWAWIALACLVVPMFASYFFDDMFSTLSHI
                     FKNPEMLELGWDSANYGFYAGGYSFLCVWGGLIICGMLLDKWGVRITGSIFVGLMIAG
                     AALVYVAISTPSIVAAGHSLTVAYIGCMLFGLGSEIAGVGVTRSIAKWFKGSYTALAM
                     GLQLAIARLGTATAFILSPRLVTAASNGMYPLSETSKPALFGLGLLLIGGILWAIFVA
                     MDARFDKLVGTTDKVVTSAEDKFKFSDIFKVLGNKHFILISLLCVFFYVCIISFKKFA
                     TSILIPRFNIELDAATWMISMIPFFTVVGTPLFGVLVDKFGKATTWMIAGSVMVFIAH
                     LIVGFAPAGVPFYGYLGIAILGTGYSLVPAAMWPSVPKIVPEKNLGTAYSLIYWIQNM
                     CMLLVPMFVGRIFKKTITAEVGSEFFAQQEVTAAVNAEYIFIVLGM"
     gene            complement(1351..2718)
                     /locus_tag="PLI69_02770"
     CDS             complement(1351..2718)
                     /locus_tag="PLI69_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG41445.1"
                     /translation="MAEKLTKLINDSPLARWSALILISLMMFFAYMFVDVMSPLKSLI
                     ENSLGWDSGVFGTYAASEYILNVAGFLIIAGIILDKMGVRFTGTLSASLMVIGAAIKF
                     IGITDWFQTTAFSVWLNSWWTALPSSAKMASLGFMIFGCGAEMAGTTVSKIIAKWFKG
                     KEMALAMGLEMAIARLGVFAVMWLAPIIATRYNASVLAPVAFCSALLFVGLICFLIFT
                     VMDRKLDNQFVEAGIKEEKSSEDEFRVKDLGKIFSSKMFWIVALLCVLYYSAIFPFQR
                     YAPNFLEVTLNIDAGTASRLFSFFPILAMILTPFLGIFLDLKGKGASMLMLGALIMIA
                     CHLSFAFLLPLYPHKWFAFMLIVILGVSFSLVPAALWPSVPKIIDEKVLGSAYCLIFW
                     VQNIGLSFVPLLIGKVLLHTGGYVAPMIIFSSFGVLAFIFSFLLKAEDRRKGYGLELP
                     NIKKK"
     gene            2874..4139
                     /locus_tag="PLI69_02775"
     CDS             2874..4139
                     /locus_tag="PLI69_02775"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOG41446.1"
                     /translation="MMTLDVVLGLQWGDEGKGKIVDVLAEKYPVIARFQGGPNAGHSL
                     HFEGNKIVLHTVPSGVFRPNATNIIGNGVVLDPIIFKGECEAIIKMGVPVRERLVISQ
                     KAHLILPTHRIIDAAQEAAKGASKIGSTLKGITPTYVDKIGRAGLRVGDILATDFVSR
                     YNTLKEQHIGYLRQFDFEYDPMQNEKEWLEAVEYLRSFKIVESEYMVNELLDSGKQVL
                     AEGAQGLMLDIDFGSYPFVTSSSTACAGASIGLGVSPKRVGKVFGIFKAYCTRVGSGP
                     FPTEIFDETGEELRSRGFEFGATTGRPRRTGWLDLVALKYAIMINGVTDLIMMKSDVL
                     DTFDTVKVATSYIIDGKNSSQVPYDSSSEVTPVYREFPGWKRDLTGVRQEGELPEEFL
                     SYVKFIEKETGVPIKIISIGPDRDQTIIR"
     gene            4153..6018
                     /gene="dxs"
                     /locus_tag="PLI69_02780"
     CDS             4153..6018
                     /gene="dxs"
                     /locus_tag="PLI69_02780"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOG41447.1"
                     /translation="MTLLENINYPKDIKDLSLKELEQLCSELREYIVSSCASNPGHIG
                     ASLGVVELAVALHRVFDTPKDKIVWDVGHQAYAHKIITGRREAFKTNRKAGGISGFPK
                     RSESEYDAFGTGHSSTSLSAALGLAIGAKMQGSSEHLVAVIGDGSMTGGLAFEGLNNA
                     GSLKTNLLVILNDNQISIDKSIGALHNHLMKVTTSRKYNRIKKSIWDRLGAGKIRNHI
                     QNLIKYIKKGILRNTSASNLFDSLGFRYFGPMDGNDVEQMIDVLSRLKEIEGPKLLHV
                     ITKKGKGFKPAEENQTIWHAPGQFDPITGNRISGPSGASKFQDVFGQTLLDLARENEK
                     IIGITPAMATGCSMNMMMCEMPDRAFDVGIAEQHAVTFSAGLAAAGMLPFCNIYSSFA
                     QRAYDNVIHDVALQELKVVFCLDRAGIVGEDGPTHHGAFDFSCFRPVPNVIIAAPLNE
                     LDLRNMMYSATLPEYGTTIIRYPRGTGNGTAWRDEPFRRIEPGRGVILQEGSDVALLS
                     MGAIGHNGTLAVKAAQEKGISVFHCDMRFLKPIDEKLLAEVCSKVSHIVTLEDGALIG
                     GLYSAVSEYVASNNIPCKVTGLGMPDRFVGQGTPEQLHKECGYDVDGILKVLLNG"
     gene            6011..>6829
                     /locus_tag="PLI69_02785"
     CDS             6011..>6829
                     /locus_tag="PLI69_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOG41448.1"
                     /translation="MAKRRHDHTRGIHNMGGESEGDFPLLTHRFWGLLIFLAVIWLMF
                     FSTFKFGSYPQQWIEALMGKLSFFIGQRMQDGWLLDFLQNGIINGIGAVLAFLPNILI
                     LFFFIFLLEESRYLPIAANLMDKYMHGIGLHGCSFIPLLMGFGCNVPAIMATRSIKRR
                     GDRILTMMMIPYIPCSARIPTFLLFSGIFFPQHRTLVMMLLYLGGILTGIVIALINKK
                     IFFRGKSEDHDTELPPLRVPNVRRALMGMWSAASEYLKKIGTVVLLAVIIVWALD"
BASE COUNT         1957 a         1417 c         1674 g         1781 t
ORIGIN      
        1 catgccgagt acgataaata tgtactctgc attaacagct gcagttactt cctgctgtgc
       61 aaagaactct gaaccaacct cagcagtaat ggtctttttg aaaattctgc cgacaaacat
      121 gggcacaagg agcatacaca tattctgaat ccagtagata agggaatatg ctgttccgag
      181 gttcttttca ggtacaatct tgggaactga aggccacata gccgccggta cgagcgaata
      241 acctgttccg aggatggcaa taccgagata accatagaaa ggaacgcctg caggagcgaa
      301 acctactatt aagtgagcta taaataccat cacggaaccg gctatcatcc aagttgtggc
      361 ttttccaaat ttgtcgacaa gaacaccgaa gagtggagta cctacaacag taaagaaagg
      421 tatcatggag atcatccagg tggcagcatc taactctatg ttgaaacggg ggatgaggat
      481 ggatgtcgca aacttcttga atgagatgat acaaacatag aagaatacgc aaagcagcga
      541 aatcagaatg aagtgcttgt tgccaagaac cttgaaaata tcggagaatt tgaatttatc
      601 ctcggcagag gttacaacct tgtctgtagt acctacaagc ttgtcaaatc ttgcgtccat
      661 tgcaacaaat atagcccaaa ggataccgcc aatcagtagg agtccaagtc cgaaaagagc
      721 aggtttgctg gtttcgctga gggggtacat cccgtttgaa gcagctgtaa caagtctggg
      781 tgatagaatg aatgcagttg ccgtaccgag tcttgcaatg gcgagttgta gtcccattgc
      841 aagtgcggtg tagctgcctt taaaccattt ggcaatcgag cgggtgacac ctactccggc
      901 aatctcgctg ccgagtccaa agagcataca tccgatatag gccacagtaa gagagtgacc
      961 tgcggccaca atggaaggag tagaaatggc cacgtagacc agtgccgcac cggcaatcat
     1021 caaacctaca aagatggagc ctgttatacg tacaccccat ttatctagaa gcataccaca
     1081 gatgatcagg ccgccccata cacagaggaa ggagtatcca cccgcataga atccatagtt
     1141 ggcggagtcc caaccgagct ccagcatttc cgggttctta aagatgtggg atagagtaga
     1201 gaacatgtca tcgaaaaagt aggatgcaaa catcggtaca accaggcacg cgagagcaat
     1261 ccaggcccat ctatgtatgt tctttttctt ggcttggatt tgttgttcag tcataaatag
     1321 ttattataaa gttaatagtt ttattcagga ttacttcttt tttatattgg gtaattcgag
     1381 gccatagcct ttgcggcggt cttccgcttt aagcagaaag ctgaatatga aggcaagcac
     1441 accgaaactt gagaagatga tcattggagc gacatatcca ccggtatgta ggagcacctt
     1501 tccgataaga aggggtacga agcttaagcc tatgttctgc acccagaata taagacagta
     1561 ggcagacccg agtacctttt cgtcaataat cttggggacg gaaggccata aagctgccgg
     1621 cacaagagaa aaagagactc cgagaatcac aatcaacata aatgcgaacc acttatgagg
     1681 gtagagaggc agcaggaatg cgaaactcag gtggcatgct atcattatga gagctcctag
     1741 catcagcatg gatgctccct ttcccttaag gtcaagaaaa attccaagga aaggagtgag
     1801 aatcattgcc agtataggga aaaagctaaa tagccttgaa gccgtaccgg cgtctatatt
     1861 cagagtcact tcgaggaaat tgggcgcata tctctggaag gggaaaatag cgctgtagta
     1921 gagtacgcag agcagagcta cgatccagaa catcttgctt gagaaaatct tgcccaaatc
     1981 cttaacccgg aattcatcct cagaactttt ttcctcttta atgccagctt caacaaactg
     2041 attgtcaagc ttgcgatcca ttacggtgaa tatcaggaag cagattagac ctacaaatag
     2101 gagtgcggag caaaatgcta caggagcaag cactgaagcg ttgtaacggg tggcaataat
     2161 aggggcaagc cacattacgg caaatacacc caggcgggct atagccatct caagtcccat
     2221 ggcgagggcc atttccttcc ctttgaacca ttttgcaata atctttgaga ctgtagtacc
     2281 tgccatttcg gcaccacacc cgaaaatcat aaaaccgagg gatgccattt tggcactgct
     2341 gggtagagct gtccaccagc tgttgagcca tacgctgaaa gctgtagtct gaaaccagtc
     2401 ggtaatgcct ataaacttga tagctgctcc gatcaccatt agtgaggcgg agagtgtgcc
     2461 tgtgaagcgc acacccattt tatccaggat gatgcctgca atgataagga atccggctac
     2521 attcagtatg tattcggatg cagcgtatgt gccgaatact ccgctgtccc acccaaggga
     2581 gttttcaatg agggatttga gcggggacat cacgtcaacg aacatgtatg cgaaaaacat
     2641 catcagagag atgagtatca gcgcactcca ccttgctaga ggtgagtcgt tgatcaattt
     2701 ggtcaatttt tctgccataa ttatcttttt taatattttt ctttgcaaag aaatgctaaa
     2761 aatagtgtat ttcaatgaaa cggcaaaata taaattgtac ccccggttat atttctacaa
     2821 ttggaatata agttgtacct tagcatgtta tttcaaaatc tatatattga taaattatga
     2881 cacttgatgt agttttggga ctccagtggg gtgatgaagg taaaggaaaa atagttgatg
     2941 tgcttgcaga gaaatatcct gtgatagccc gttttcaggg gggacccaat gcaggacatt
     3001 cccttcactt tgaaggtaat aaaatcgtat tgcatactgt tccttcaggc gttttccgcc
     3061 ccaatgccac aaatatcata ggtaatggcg ttgtactgga tcctattatt ttcaaaggag
     3121 aatgtgaagc gatcataaaa atgggtgttc ccgtgagaga gcgacttgta atttcccaaa
     3181 aggcacatct tattctaccc acacatcgca taatagatgc agcccaggag gctgccaaag
     3241 gggcatcaaa gattggatcc actctcaagg gcatcacacc cacttatgta gataagatag
     3301 gtcgtgcggg acttagggta ggtgatatcc ttgcaactga ctttgtctcc cgatacaata
     3361 ctctcaaaga gcaacatatt ggatatctcc gacagtttga ttttgaatat gatcccatgc
     3421 aaaatgagaa agagtggctc gaggcagtgg aatatcttag gagtttcaag attgtagaga
     3481 gtgaatatat ggtaaacgaa ctgctagact ccggcaaaca agttttggca gagggggcac
     3541 aaggcctgat gctcgacatc gatttcggct catatccctt cgtaacctca tcatctactg
     3601 cctgcgcggg tgcttctatc ggacttgggg tttctccaaa acgtgttggc aaggtatttg
     3661 gaatatttaa agcctattgt acacgagtag gtagtggccc tttcccaacg gagatttttg
     3721 atgagacggg ggaagagctc cgtagcaggg gatttgagtt cggtgctact accggtcgac
     3781 cccgccggac cggctggctt gatcttgtgg ctttgaaata tgctattatg attaacggtg
     3841 tgacggactt gataatgatg aaatctgatg ttctggatac attcgatacg gttaaggttg
     3901 caacatcgta cataattgat gggaagaatt cctctcaagt accttacgat tcctcttcag
     3961 aagtaactcc cgtttatcga gagttccccg gttggaagag agatcttacc ggagtacgcc
     4021 aggagggaga acttcctgaa gaatttttaa gttatgtgaa gtttatagaa aaagagacgg
     4081 gggttcccat aaaaataata tccatcggcc ccgacaggga ccaaaccata atcagatagg
     4141 agagaagata cggtgacgct acttgaaaat ataaattatc ccaaagatat aaaagatttg
     4201 tcgctaaaag agttggagca gttgtgttcc gagttgagag aatatattgt cagctcctgt
     4261 gcttcaaatc ccggtcacat aggcgccagt ctcggagttg tagaacttgc ggtagcactg
     4321 catagagtgt tcgacacccc gaaagataaa atagtctggg atgtgggcca tcaggcatac
     4381 gcacacaaga taattacagg cagacgtgag gcctttaaga ccaaccgcaa ggcgggaggt
     4441 atcagtggtt ttccgaaacg ttcggagagc gaatatgatg cattcggtac cggacactca
     4501 tctacatccc tttcagcagc gcttggattg gccatagggg ctaagatgca gggaagtagt
     4561 gagcatttag ttgcagtaat aggtgacggt tccatgaccg gcggacttgc tttcgaaggg
     4621 ctcaacaatg caggctcact aaagacaaat ctcctggtga tcctcaatga caatcagata
     4681 tcgatagata aaagtatagg tgcgctgcac aaccacctaa tgaaggtgac taccagtcgc
     4741 aaatacaatc gaattaagaa gagtatatgg gatcgcctcg gcgccggaaa gatccgtaac
     4801 catattcaaa atcttattaa atacatcaaa aagggtattt tacgcaatac cagcgcgagc
     4861 aaccttttcg actctcttgg tttcaggtat tttggaccga tggatggcaa cgatgttgag
     4921 caaatgatcg acgtactctc ccgtcttaaa gagattgagg ggccaaaact tctacatgta
     4981 ataactaaga agggaaaagg ctttaagccg gctgaagaga accagacgat ttggcatgct
     5041 cctggtcagt ttgaccccat tactggtaat agaatttcag ggccaagcgg ggcatccaaa
     5101 ttccaggatg tctttggaca gacgctgctc gatcttgcga gggaaaatga aaaaatcata
     5161 ggtattaccc cggctatggc taccggttgc agcatgaata tgatgatgtg tgagatgccg
     5221 gatcgtgcgt ttgatgttgg tattgccgag cagcatgcag ttacattctc agcaggactg
     5281 gcggctgcgg gtatgttgcc cttctgtaat atctactcct catttgctca gcgtgcttat
     5341 gacaatgtca tccatgatgt ggctcttcag gagcttaagg ttgttttctg tctcgatagg
     5401 gcgggtattg taggagaaga tggtccgact catcacggtg cttttgactt ttcatgtttc
     5461 agaccggttc ccaatgtgat aattgctgcc cctctcaatg aacttgatct ccgtaatatg
     5521 atgtattccg ccaccctacc ggagtatggt actactataa taaggtatcc ccgtggtacg
     5581 ggtaatggta ctgcctggag ggatgaaccc tttcgcagaa ttgagcctgg acgtggagtg
     5641 atcctacagg agggaagtga tgtggcttta ttgagcatgg gagccatagg tcataatggt
     5701 actcttgcag taaaggcggc gcaggagaag ggaataagtg tatttcactg tgacatgagg
     5761 tttcttaagc cgattgacga aaaattactg gccgaggttt gctccaaagt gagtcatata
     5821 gtgactcttg aagatggtgc actcatagga ggtttatata gtgcagtatc ggagtatgtt
     5881 gcctcaaaca atattccctg caaggtgaca gggctcggga tgccggatag atttgttggt
     5941 cagggtacac ccgagcaact ccacaaggag tgcggatatg atgttgatgg aatattaaaa
     6001 gtgcttttaa atggctaaac gcagacacga tcataccaga ggtatacata atatgggagg
     6061 agagagtgag ggcgactttc ccttattgac ccacagattt tggggtcttc tgatcttttt
     6121 agccgtcatc tggctgatgt tcttctctac ttttaagttc ggttcctacc ctcagcaatg
     6181 gattgaggct cttatgggaa aattgtcgtt ctttatcggg caaaggatgc aggatggatg
     6241 gcttctggat tttcttcaaa acggtataat aaatggtatt ggcgcggtgc tggcattcct
     6301 gcccaacata cttatactat tcttctttat atttttactt gaagagtcaa gatatttacc
     6361 catcgcagct aatttgatgg acaaatatat gcacggaata ggacttcacg gctgttcatt
     6421 tattcctttg cttatgggct ttgggtgcaa tgtgccggcc ataatggcaa caaggagtat
     6481 taagagacgc ggagatcgta tccttacgat gatgatgatc ccctacatcc catgtagtgc
     6541 ccgtatcccg acattcctac ttttttccgg aatatttttc ccgcaacaca ggactctggt
     6601 aatgatgctc ctctatttgg gtggtattct gaccggtatt gtgatagcac ttataaataa
     6661 aaagatattt tttaggggaa aatcggagga tcacgataca gagttacccc cattgagggt
     6721 tcccaacgtc agaagggctc ttatgggtat gtggagtgcc gcgtcagagt atctcaagaa
     6781 gatcggtact gtagtgctgt tggcagtgat aattgtatgg gccctggat
//
LOCUS       DAOSIE010000056         6777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_10199_length_6777_cov_3.661559, whole genome shotgun sequence.
ACCESSION   DAOSIE010000056 DAOSIE010000000
VERSION     DAOSIE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10199_length_6777_cov_3.661559"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1116)
                     /locus_tag="PLI69_02790"
     CDS             complement(109..1116)
                     /locus_tag="PLI69_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOG41449.1"
                     /translation="MDNSQYKNVFVFAEQRDGVVQSVALELIGKARDLAADLGEKVFA
                     VLLGKGITPSAQKLVEYGADTVIVVDHEYLKDYVTEQYTQAMYQVLVEYKPSIVMFGA
                     TTIGRDLAPRVAARLGTGLTADCTKLEISEDTKEFQMTRPAFGGNLMATIMCPNHRPQ
                     MSTVRPGVMQKCTPDTSRKGEIINFVPKFDSSKFTVKLVKNVKEQKASVDIAAAKILI
                     SGGRGVGCTEGFGKLRDLAAVIGAEVSSSRAMVDAGNMPHDRQVGQTGKTVRPDVYFA
                     LGISGAIQHLAGMEESECIIAVNKDKFAPIFAVADLGIVSDLNAVVPLLTERLKKEMA
                     K"
     gene            complement(1137..1916)
                     /locus_tag="PLI69_02795"
     CDS             complement(1137..1916)
                     /locus_tag="PLI69_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOG41450.1"
                     /translation="MKILVCVKQVPDTTEIKIDPVTKTLIREGVPSIMNPDDKGGLEL
                     ALQFKDKFGAEVTVITMGPPQADVVLREAFAMGADRAILLTDRKFAGADTLATSHALA
                     GAARKLHWDLIITGRQAIDGDTAQVGPEMAEHLALPQISYVAGVEYDGKTFTVKKETE
                     EGYQMLEFEGKGLLTVLASAFKARYMSVGGIVDAYDKEVEIWGADRIDLDESKLGLKG
                     SPTKVVKSFAKELKQPGVIHEVDAEQAVELIVSKLKEKHII"
     gene            complement(1931..3091)
                     /locus_tag="PLI69_02800"
     CDS             complement(1931..3091)
                     /locus_tag="PLI69_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOG41451.1"
                     /translation="MNFNLTKTQELFRQMIREFADKEVKPLAAEIDEQERFPEETVKK
                     MAKLGLFGIPVPKEYGGAGGDNIMYSIAVEELSRCCATTGVVVSAHTSLCLVPILEFG
                     TEEQKRKYLPKLASGEWLGAFGLTEPNAGTDSSAQQTTTVLDGDEWVLNGSKIFITNA
                     GQAGLYLIIAMTDKSQGNRGISAFLVEANTPGFCVGKKELKMGIRGSATAELIFDNCR
                     IPKENLLGQVGRGFRIAMKTLDGGRIGIAAQALGIAQGALDETVKYTKERKQFGRAIA
                     KFQNTQFQMADMKTKIEAARLLIRSAAFKKDESIKNDRINYSADSAMAKLYASEVAME
                     VTTKAVQFHGGYGYTREYPVERMMRDAKITEIYEGTSEVQRMVIAGDLFGKI"
     gene            complement(3106..4317)
                     /locus_tag="PLI69_02805"
     CDS             complement(3106..4317)
                     /locus_tag="PLI69_02805"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOG41452.1"
                     /translation="MKKVYIVAAKRTAIGKFLGSVANFTPGELGAVVIKNLIEETGIK
                     PEQVDEVIGGNVLPGGQSQGVMRQASIKAGIPAEVPAYGVNMVCGSGMKAVMTGYNDI
                     LVGDADIVIAGGTEVMSNAGFILGGNLRAGSKMGDLKAVDHMVNDGLTDAFNNYHMGI
                     TAENIAEKYGITREEQDEFGFASQQKAIKAIDSGAFAKEIVPVEIVTRKETIIFNEDE
                     YPNRTSTYEKMAKLRPAFKKDGTVTAGNASGINDGASFVMLVSEEKMKELGLKPMVEI
                     VATGQGGVDPSIMGMGPVPAIRSVLKKSGMKLQDMELIELNEAFAAQSLGVIHELIKE
                     HGVTKEWIDERTNINGGAIALGHPIGASGNRIIVTLIHEMIKSGKKLGLASLCIGGGM
                     GTAIILRNCTK"
     gene            complement(4338..>4867)
                     /locus_tag="PLI69_02810"
     CDS             complement(4338..>4867)
                     /locus_tag="PLI69_02810"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOG41453.1"
                     /translation="VTLQSENGLVGMGPAPEEGREDKDYINAGGGYITYNPGAYACSS
                     ADSFLIIRGGHVDATFLGAMQVDEKGNLANWMIPGKKTPGMGGAMDLIVGANHVIVTM
                     EHTARGNHKIMKECTLPLTAAGQVNMIITEMGVMKVTPKGLVLTEINPEFTVEQVQEA
                     TEAKLIIAEDLKPMC"
     assembly_gap    4868..4877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4878..4992)
                     /locus_tag="PLI69_02815"
                     /pseudo
     CDS             complement(4878..4992)
                     /locus_tag="PLI69_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458079.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
     gene            complement(5023..5814)
                     /locus_tag="PLI69_02820"
     CDS             complement(5023..5814)
                     /locus_tag="PLI69_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOG41454.1"
                     /translation="MSCGLYSMSDKEFDKTLDLTKVKPYGDTMNDGKVQLSFTLPVPA
                     GEEAIEAAKQLLKSMGFDNPLVVYYRELTPGYTFFNCYGSTVHTVDFTSIYVPKVEST
                     VMDMNQTDDFIRENIGRKLVVVGASTGTDAHTVGIDAIMNMKGYAGHFGLERYDMIEA
                     YNLGSQVPNEEFLAKAIELKADALLVSQTVTQKDVHIKNMTELVELMEAEGLRDKMIL
                     VCGGPRISHELAKELGYDAGFGMNSYADDVASFIVHEFVKRMSVK"
     gene            complement(5816..>6777)
                     /locus_tag="PLI69_02825"
     CDS             complement(5816..>6777)
                     /locus_tag="PLI69_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOG41455.1"
                     /translation="TTEGFGGTYATQENFRIMREALDKVGEELGRYIRLCNYCSGLCM
                     PEIAIMGAFEGLDVMLNDALYGILFRDINMQRTFVDQYFSRIINGFAGVIINTGEDNY
                     LTTSDAVEAAHTVLVSDLINERLALLAGLPEEQMGLGHAFEMDPELENGFLYELAQAQ
                     MTREIFPKAPLKYMPPTKFMTGNIFKGHIQDALFNMIGIWTKQGIQLLGMPTEAVHTP
                     FMSDRYLAIENAKYIFGNMKNIGDEVEFKEGGIIRNRAAKVLDDAVELLEKIAAEGLF
                     DALEKGIFGDVKRPKNGGKGLDGVVQKGENYMNPFIKLMKGVK"
BASE COUNT         1726 a         1726 c         1390 g         1925 t
ORIGIN      
        1 tttacgcata acaaaacccc gaaagttttt tgtctaactt tcggggttca tgtcataatt
       61 gtaccgggct ctattcttct aataatatac cttagaatca gctaaggatt attttgccat
      121 ctcttttttg aggcgttcgg tgagtagcgg aaccacagcg ttaaggtcgc ttactatacc
      181 caaatccgca actgcaaaaa taggggcgaa tttatctttg ttgacggcaa ttatacattc
      241 cgactcctcc atacctgcaa ggtgttgtat tgcaccggag attccgaggg cgaaatagac
      301 atcgggacga acggtcttac ctgtctgacc tacctgacgg tcgtggggca tattgcctgc
      361 atccaccatt gcacgtgaag atgaaacctc agcaccgata actgctgcca ggtcacggag
      421 ttttccaaat ccttcggtac aacccactcc cctaccgcct gaaatgagga ttttagccgc
      481 tgcaatatcc acgctggctt tctgctcctt cacattttta acaagcttca cggtaaattt
      541 tgatgagtcg aatttgggaa cgaaattaat gatttcaccc ttacgggagg tatccggagt
      601 gcatttctgc atcacaccgg ggcgtaccgt tgacatttga ggcctatggt tgggacacat
      661 gatagtggcc atcaggttgc ctccgaatgc cgggcgcgtc atctgaaatt ccttcgtatc
      721 ttccgaaatc tctaatttgg tacagtcggc tgtaagaccg gtaccaaggc gtgcagccac
      781 gcggggtgcc aggtcacgac ctatagttgt tgcaccgaac attactatgg agggtttgta
      841 ctccacaagc acctgataca ttgcctgcgt atattgctcg gttacataat ccttgaggta
      901 ttcatggtca acaacgatca ccgtatcggc cccatactct acaagtttct gtgcggaagg
      961 ggttattcct ttgcctaaaa gtactgcaaa gaccttttct ccaagatcgg cagcaaggtc
     1021 acgcgccttg ccgataagtt caagagcaac agattgtact acaccgtctc tttgctctgc
     1081 aaagacaaaa acatttttat attgtgaatt atccatattc agtcaaattt aacgggttag
     1141 atgatatgtt tctcttttag tttgcttaca atcaactcaa ctgcctgctc ggcatcaacc
     1201 tcatggatga ccccgggctg tttaagctcc tttgcaaatg actttacaac cttggtagga
     1261 gagcctttca gaccgagttt cgactcatcg aggtcgatcc tgtctgcacc ccagatttca
     1321 acttctttgt cgtaagcatc aacaatacct cctacactca tataacgagc cttgaaagct
     1381 gaagccagta cagtaagaag gcctttaccc tcaaactcca acatctgata accctcttcg
     1441 gtctccttct tgacagtaaa agttttaccg tcatactcca caccggcaac ataggagatc
     1501 tgaggaagag ccagatgttc tgccatttct ggacctacct gagcggtatc accgtcaata
     1561 gcctgacgac ctgtgattat aagatcccaa tgaagtttcc ttgccgcacc ggcaagagca
     1621 tgagaagtag caagtgtatc ggcacctgca aacttgcggt cagtgaggag aatagccctg
     1681 tcagcaccca ttgcgaaagc ttcacgcagt acgacatctg cctgaggggg tcccattgtg
     1741 ataactgtaa cctctgcccc aaacttatcc ttaaattgga gagccaactc aagtcccccc
     1801 ttgtcgtctg gattcatgat ggaaggaaca ccttcacgga tcaatgtctt tgttaccgga
     1861 tcaatcttaa tctctgtggt atccggaact tgttttacac ataccaaaat cttcatatat
     1921 attcctccta tcatatttta ccaaacaaat cacctgcaat taccatacgc tgaacctctg
     1981 aagtaccttc ataaatctcg gtaatcttag catcacgcat catcctctca actggatatt
     2041 cgcgggtata accatatcca ccgtggaatt gaacggcttt agtcgtaacc tccatagcca
     2101 cttcggaagc atagagtttc gccatggcag aatcggcaga gtagtttatc ctatcgtttt
     2161 tgatggattc gtctttctta aaggcagcac tgcgtataag aagacgggca gcctcgatct
     2221 ttgtcttcat atcagccatc tggaactgtg tattctggaa tttggcaatt gcacgaccga
     2281 actgctttct ctccttagta tatttgacag tctcgtccaa agctccctgg gctataccaa
     2341 gtgcttgtgc agctatacct atacgtcctc cgtcaagggt tttcatagca atcctgaagc
     2401 ccctgccaac ttgtcccagg agattttcct tgggaatacg gcaattatca aagataagtt
     2461 ccgcggtagc gctgcccctg atacccattt tgagctcttt ctttcccaca cagaatccgg
     2521 gagtgtttgc ttctacgaga aatgctgaga tacctctgtt accctgagac ttgtccgtca
     2581 tggcaataat caaataaagt ccggcttgac cggcattcgt gatgaaaatt ttactgccat
     2641 tcagtaccca ttcatcacca tcgaggacag ttgtagtctg ctgagctgaa gagtcggtac
     2701 cggcattggg ctctgtaagt ccgaatgcac cgagccactc accggaagcg agtttcggca
     2761 aatactttct cttttgctcc tctgtgccaa attccaaaat gggaaccaga cagagggaag
     2821 tgtgtgcgga aactaccacg cctgtagtgg cgcagcagcg tgacaactct tcaacagcta
     2881 tggaatacat aatattgtcc ccacctgcac cgccatattc tttgggtacc gggataccga
     2941 agagaccaag tttggccatc ttctttacag tctcttcggg aaaacgttcc tgctcgtcaa
     3001 tttccgctgc gagaggtttg acctctttgt cagcgaactc acggatcatt tgtctgaaga
     3061 gttcttgagt ctttgtcaaa ttaaaattca tgttttgtgt ttgtgttatt ttgtacagtt
     3121 acgtaaaatt attgctgtcc ccataccacc accgatacag agtgatgcaa gaccgagttt
     3181 cttgccgctt tttatcattt cgtgaataag tgtaacgata atacggttgc cggatgctcc
     3241 gatagggtga ccgagagcta tggcaccgcc atttatgttt gtacgctcat caatccactc
     3301 ttttgtaaca ccatgctctt tgatcaattc atggattaca ccgagagact gagctgcaaa
     3361 tgcctcattg agctcgatca gctccatatc ctgaagtttc ataccggatt ttttgaggac
     3421 gctgcgaata gcgggaacgg gacccatacc catgatggaa ggatcaacac cgccctgacc
     3481 tgttgcaact atttcaacca taggtttcag accgagttct ttcatctttt cctcagatac
     3541 gagcatgaca aatgaagcac cgtcatttat gcctgaagca tttcctgccg ttacagtacc
     3601 atccttcttg aatgcaggcc tgagtttggc catcttttca taggttgaag ttctgtttgg
     3661 atactcatct tcgttgaaaa tgatggtctc ctttctggtt acgatctcta cgggaacgat
     3721 ttcctttgca aatgcacctg agtcaatggc ctttatagct ttctgctgtg aagcaaaacc
     3781 aaattcatct tgctcctcac gggtaattcc atatttttcc gcaatatttt cagcggttat
     3841 acccatgtgg taattgttga aagcatctgt aaggccatcg ttgaccatgt ggtctacagc
     3901 tttaagatcg cccatcttgc tgcccgcacg gagattgcct ccaaggatga agcctgcatt
     3961 ggacataacc tcagtgccac ctgcgattac gatatcggca tcacctacaa gaatgtcatt
     4021 gtatccggtc ataaccgcct tcatgccgct accgcatacc atattgacac cataagcggg
     4081 aacttccgcg ggaatgccgg ccttgatgga ggcctgacgc ataacgccct gcgactgacc
     4141 gccggggaga acattaccgc ctattacctc atctacctgc tctggcttga taccggtctc
     4201 ttcgattaga tttttaatga caactgcgcc caactctccg ggagtaaagt tggcgacgga
     4261 acctaaaaac ttacctattg cagtcctttt ggctgctacg atatatacct ttttcatctc
     4321 tatataattt taaaaattta acacataggt tttaaatcct cagcaattat gagctttgct
     4381 tcggtggctt cctgtacctg ttcaaccgtg aattcaggat tgatctcagt aagtaccagt
     4441 cctttgggag taactttcat cacacccatt tcagtaatga tcatgttgac ctgaccggcc
     4501 gcagtcagag ggagcgtaca ctctttcata atcttgtggt tgccccttgc agtatgctcc
     4561 atcgtaacaa tcacgtgatt ggcacctaca atcaggtcca tagctccgcc catccccgga
     4621 gtctttttgc ccggaatcat ccagttggca agattacctt tctcatctac ctgcatagca
     4681 ccaaggaaag ttgcatctac gtgacctccc ctaatgataa gaaatgaatc tgctgaagaa
     4741 caagcatagg cgccgggatt gtaggtgata tagccaccac cggcattaat ataatccttg
     4801 tcctctctac cctcttcggg ggcaggtccc atacctacaa gtccattctc agactgaaga
     4861 gtcacatnnn nnnnnnngaa gatatgaggg aattgcggtg ggaataccga tacccagatt
     4921 taccacatca ccatcttgaa gttcgagagc tgcccttctg gcaataactt ctctcatttg
     4981 atttctgtcc atcatatata gttttttata attaataatc ggttatttta ctgacattct
     5041 cttgacaaac tcgtgcacga taaaagaggc cacatcatct gcataactgt tcatgccgaa
     5101 ccctgcatca taccccagct cttttgcaag ttcatgggaa atgcgggggc ctccgcaaac
     5161 aaggatcatc ttgtcgcgca gaccttccgc ctccatcaac tcaacgagct cggtcatatt
     5221 cttgatatgg acatctttct gtgtaactgt ctgggaaacg agcagcgcat ccgccttgag
     5281 ttcaatcgct tttgcaagaa attcctcatt gggtacctga ctgccgagat tataggcctc
     5341 aatcatatca taacgctcaa ggccgaagtg tccggcatag cctttcatat tcataattgc
     5401 atcgatgccc acagtatgag cgtcagtgcc cgtagatgcc cccactacca ccagtttacg
     5461 acctatattc tcacgaatga agtcatctgt ctgattcata tccattacag tcgactccac
     5521 tttgggtaca taaatcgagg taaaatccac cgtgtgaacc gtactgccgt agcagttgaa
     5581 aaatgtatat cccggagtca gttcgcggta atatactacc agagggttat caaaacccat
     5641 actcttcagg agctgctttg cagcctcaat agcctcttca ccggcaggaa cgggaagggt
     5701 aaaactgagt tgtaccttgc catcgttcat cgtgtcgccg tagggcttta ccttggtcag
     5761 atcaagtgtt ttgtcaaatt ccttgtcaga cattgaatat aatccgcaac tcatattatt
     5821 ttactccttt cattaatttt ataaatggat tcatatagtt ttcgcccttc tgaactactc
     5881 cgtcaaggcc tttaccgccg tttttagggc gtttcacatc cccgaaaata cccttttcaa
     5941 gagcatcgaa aagaccttcg gcagcaattt tctccaaaag ctccacagca tcatccagta
     6001 ctttagccgc cctgtttcgg attataccac cctctttgaa ctccacttca tcaccaatat
     6061 tcttcatgtt tccaaatata tatttggcat tttctattgc aaggtaacga tcactcataa
     6121 agggagtatg aactgcttcc gtaggcatgc caagtagctg aatgccctgt ttggtccaga
     6181 taccgatcat attgaaaagc gcatcctgga tatgtccttt aaagatattt ccggtcatga
     6241 acttggtggg aggcatatat ttcaggggag ccttggggaa aatctcacga gtcatttgcg
     6301 cttgtgcaag ttcgtagaga aatccgttct caagttcggg atccatctca aatgcatgtc
     6361 cgagacccat ctgctcctcg ggaagacctg ccagcagtgc aagccgttca tttatcaaat
     6421 ctgatacaag tacggtatgg gcggcctcca ccgcgtctga agtggtaagg tagttgtcct
     6481 cacctgtatt tatgatgacg cctgcaaacc cattgatgat acgtgagaaa tactggtcca
     6541 caaaggtcct ctgcatattg atatccctga aaagaatacc atatagcgca tcattgagca
     6601 tcacatcaag accttcaaag gctcccataa tggcaatttc aggcatacag agtcccgagc
     6661 agtagttgca gagtcggata tagcgtccca actcctctcc taccttatcg agagcttcac
     6721 gcatgatgcg gaagttctct tgtgtagcgt atgtgccgcc aaaaccttca gtagtgg
//
LOCUS       DAOSIE010000057         6679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_10404_length_6679_cov_4.357337, whole genome shotgun sequence.
ACCESSION   DAOSIE010000057 DAOSIE010000000
VERSION     DAOSIE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10404_length_6679_cov_4.357337"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..670)
                     /locus_tag="PLI69_02830"
     CDS             complement(158..670)
                     /locus_tag="PLI69_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG41456.1"
                     /translation="MALNKVLLIGNVGRDPEIRHLEGGASVASFSLATTERFRDRNGE
                     TKEITEWHNIVAWRQLADLAGRFIKKGSQIYVEGRIRSRSWDDANGQKRYITEILADT
                     IQLLGRRDDTLPRDGAWDDSAGTSRPSDSPAHHVTKPASKSAPQPASQSEQFVTSDDI
                     MSDGPDDLPF"
     gene            complement(686..1348)
                     /locus_tag="PLI69_02835"
     CDS             complement(686..1348)
                     /locus_tag="PLI69_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41457.1"
                     /translation="MLKTSILTATLSVAWQWVVYALLIIFIVVTIILALILFRIKRRE
                     MRIKEKISEQLRKKEADEEYLAQALNNVIQMVIGIMDCISLSPENANLLHSKIRSYFL
                     TTLRDRNNIFYEMDYLADKCQYGLISRLKEEYPSLTKDDVTLCSLISMGFPTHAIATI
                     FRLTNEMSIYNKRERLRTRLEMAEGANLKDFLEEKAEMLRNQKAFLYANIFSNLCKKD
                     YI"
     gene            complement(1368..>1764)
                     /locus_tag="PLI69_02840"
     CDS             complement(1368..>1764)
                     /locus_tag="PLI69_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOG41458.1"
                     /translation="TADACGADCVLVCDPLADIFNPNVIRASTGAFFSVNSVCCTSEE
                     ALRWLKMSNISILTTQLQDSSPYHDSDMRGAVAIVFGREDRGLSDFWKEHSDEKIRIP
                     MAGICDSLNVSVSAAVICFEALRQRKKIT"
     assembly_gap    1765..1774
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1775..2176)
                     /locus_tag="PLI69_02845"
     CDS             complement(<1775..2176)
                     /locus_tag="PLI69_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOG41459.1"
                     /translation="MMTAEKITSARNPRIKELILLRSKSRERRKRALFVVEGEREVIR
                     AIESGYMPLSIFYSPAYIDSPKIPQVAGNVELFEVPANIYDKIAYRGGTEGIIAVMQV
                     KQLKLDAISLSDNPLIIVLESLEKPGNLGAIL"
     gene            complement(2176..>3214)
                     /gene="lysS"
                     /locus_tag="PLI69_02850"
     CDS             complement(2176..>3214)
                     /gene="lysS"
                     /locus_tag="PLI69_02850"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOG41460.1"
                     /translation="LRYRMRYVDLIVNPQVRETFVKRTKIVSTMRDFFNEHGYLEVET
                     PILQPIPGGAAARPFITHHNALDIPLYLRIANELYLKRLIVGGFTGVYEFAKNFRNEG
                     MDRTHNPEFTCMEIYVAYKDYIWMMEFVEQLVERIAIALNGKTKFDVWGHEIEFKKPY
                     RRLPMIDAIKEYAGVDITGMNEAQLRKACDDLNIPVSPSMGVGKLIDAIFSEYCEKHL
                     IQPTFITDYPVEMSPLTKRHRNNPAITERFELFVNGKEVANAYSELNDPIDQLDRFHD
                     QVKLQEKGDDEAMFIDLDFVRALEYGMPPTSGLGMGIDRLIMLMTNSTSIQDVIFFPQ
                     MRPEGGHKKID"
     assembly_gap    3215..3224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3225..3696)
                     /locus_tag="PLI69_02855"
     CDS             complement(<3225..3696)
                     /locus_tag="PLI69_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOG41461.1"
                     /translation="MEITDLNEQELNRRAALQQLKDLGIDPYPAEEFHINATSREIKE
                     GYDAEKANFNDISIAGRIMSRRIMGAASFVELQDECGKIQVYIKRDEICEGEDKTLYN
                     TVFRKLLDIGDIIGIKGYAFITQTGQLSIHAKSLTLLSKSLRVLPIVKEKEGELY"
     gene            3834..5546
                     /gene="recJ"
                     /locus_tag="PLI69_02860"
     CDS             3834..5546
                     /gene="recJ"
                     /locus_tag="PLI69_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOG41462.1"
                     /translation="MEPGNPALVRQLTQELGIDSVLANLLVQRGIDTFAKAREFFRPD
                     LSMLHDPFLMKDMDKAVERLRKAVVKRETILIYGDYDVDGTTAVALVYSFMKNLTNRV
                     EYYIPDRYDEGYGISYKGIDTARDHNCTLIIALDCGIKAIEKVNYAKSLGIDMIICDH
                     HLPDEKLPDAVAVLDPKRQDCNYPFDDLSGCGVGFKLVQGYAQQYDIPFDNIVSLLDL
                     LVVSIASDLVSVTGENRVLAYYGLKQLNENPREGLQTIIKLSGLEKHRITIDEIVFKI
                     GPRINAAGRMESGKTAVDMLTSRDEDESKEIGAAINAHNNDRKKVDREITEEALKMVK
                     SIPGFENKKSTVVYNPSWHKGVVGIVASRLVEAYYRPTIVLTQSNGLITGSARSIQGF
                     DLYEAIESCSDLLENFGGHMYAAGLTLKEENLQKFTERFETYVASKIDDVILTPIINI
                     DSYLDFKQITPKFFRLLKQFQPFGPGNLSPVFITENVYDNGNGRRVGSAEAGMKHLKL
                     ELIQEDEPYRYISAIAFNLSDNFEHIQGGNPVDICYSIAENYYRGIANIQLRVKDIKE
                     KELF"
     gene            5653..6075
                     /locus_tag="PLI69_02865"
     CDS             5653..6075
                     /locus_tag="PLI69_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41463.1"
                     /translation="MKKLLSLILLSLMVASCDPLEELTIDFELKGKWLGGIRYEIKTE
                     IDVLAKSLHFNENSKSCTVVTGLSFLNTIDVEDLVVRPNGENQLILTKRGNKEPLYRL
                     YFTKGNKDSFEMKWENHTNLEKEYIPDKSFSVIMKPYT"
     gene            6137..6277
                     /locus_tag="PLI69_02870"
     CDS             6137..6277
                     /locus_tag="PLI69_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41464.1"
                     /translation="MKKIVLLALLSIIVVSCDLIGGINKKYDLNGNWAGEIKYEIMSE
                     ND"
BASE COUNT         1958 a         1434 c         1391 g         1876 t
ORIGIN      
        1 gtacgggagc atacgctttc caggaagagc tgttacctac tgaccaagcg aaagatttct
       61 ttgaaaagaa ataaaggaaa agatataaag gaaagtagag tagacagata aaaatgcgac
      121 ctttttcagg tcgcattttt tattctatta caatctgcta gaatggcaga tcatcaggac
      181 cgtcagacat gatatcgtct gaagtaacaa attgctctga ctgtgaagcc ggttgtggag
      241 cagactttga tgcaggtttc gtaacgtggt gtgcaggcga atcgctcggt ctggaagtac
      301 ccgcagaatc atcccacgca ccatctcttg gcaatgtgtc atccctacgc ccaagaagct
      361 ggatcgtatc agccaaaatc tctgttatgt agcgtttctg cccgttggca tcatcccagg
      421 aacgacttct gatcctacct tccacataga tctgacttcc ttttttgata aatctgccgg
      481 ccaaatcagc cagctgtctc caggcaacta tgttgtgcca ttcagtaatt tctttggtct
      541 ctccattacg gtctctaaat ctctccgttg ttgcaaggga gaatgaggca acggatgctc
      601 caccttcgag atgcctgatt tcaggatctc tccccacgtt gccgataagt aaaactttgt
      661 tcagtgccat atttcaaaaa tttttctaaa tatagtcttt tttgcagaga tttgaaaaaa
      721 tatttgcata taaaaaagct ttttgatttc ggagcatctc cgccttctcc tccaggaaat
      781 ccttcagatt agccccttct gccatttcca gacgtgttct aaggcgttct cgcttgttgt
      841 agatactcat ctcattggta agtcggaaga ttgtggctat ggcatgtgtg ggaaagccca
      901 tagaaatcag gctgcataac gtcacgtcgt cctttgtaag tgagggatat tcctctttta
      961 atcgtgagat aagaccatat tgacatttgt cagccaaata gtccatttcg taaaaaatat
     1021 tatttctatc tctaagggta gtcaaaaaat aagatcgaat cttcgaatgg agtaaattag
     1081 cattttccgg actcagagag atgcaatcca taataccaat gaccatctga ataacattat
     1141 ttagggcttg ggccagatat tcttcgtctg cctccttttt tctgagttgc tctgagatct
     1201 tctcctttat tcgcatctcc ctgcgtttta tgcgaaagag aattagtgcc agaatgattg
     1261 ttactacaat aaaaataatc agtaaggcat agacaaccca ttgccacgcc accgagagag
     1321 tggcggtaag aattgaagtt tttaacatgc ttgttagttt taattagtta ggtgatcttc
     1381 tttcgttgtc tcagggcttc aaaacagata acggctgcag agaccgagac attgagcgaa
     1441 tcacagattc cggccattgg aatgcgaatt ttctcatctg aatgctcttt ccaaaaatct
     1501 gaaagacccc tgtcttccct accgaaaact attgcgacag ctcctctcat atcactatca
     1561 tggtagggtg aggagtcttg tagctgggtt gttaaaatgg agatattaga cattttgagc
     1621 cacctgaggg cctcttccga agtgcagcat acagagttga cgctaaagaa ggcaccggta
     1681 ctcgctctta ttacattagg attgaaaata tctgccaaag gatcacatac caatacacaa
     1741 tcggcgccgc acgcatcggc agtcnnnnnn nnnnaagtat ggctccaagg tttccgggtt
     1801 tttcaaggga ttccagaact attatcaatg ggttgtcact caaagaaatt gcatccaatt
     1861 tcagctgttt cacctgcatc acggcaatta ttccctcggt tccaccacga taagcaatct
     1921 tgtcataaat attcgcagga acttcgaaca actctacgtt accggcaact tgcggtattt
     1981 ttggactgtc aatatacgca ggagagtaaa aaatagagag aggcatatag cccgattcga
     2041 tggcccttat tacttcgcgc tccccttcta ctacgaaaag agcccttttc cgacgctccc
     2101 tggacttgct ccggagcaga atgagctctt ttatgcgagg attacgggcg gaggttatct
     2161 tctctgccgt catcatcagt cgattttctt gtgaccgccc tccggcctca tttgagggaa
     2221 gaaaataaca tcctgtatgg aagtggagtt ggtcataagc ataataagac ggtcaatgcc
     2281 catacccaga cccgaggtag gtggcatacc atactctaaa gcacgaacaa agtccagatc
     2341 tatgaacata gcttcatcat cacccttctc ctgtagtttg acttgatcgt gaaacctatc
     2401 gagctggtca atcgggtcat tcagttcgct ataggcattt gctacctctt tgccgttgac
     2461 aaagagttcg aatcgctcag ttattgccgg attgtttctg tgacgtttgg taagaggaga
     2521 catctctacg gggtaatctg tgatgaaggt aggctggatc aaatgctttt cgcaatattc
     2581 gctgaagatg gcatcaatca atttgcctac tcccatagag ggagaaacgg gaatattaag
     2641 atcgtcacat gcctttcgca actgcgcctc attcatgccg gtaatatcta caccggcata
     2701 ctcctttatg gcatcaatca taggaagacg cctgtagggc ttcttgaact ctatttcatg
     2761 gccccaaaca tcgaatttgg tcttaccgtt gagtgcaatg gctatcctct cgacaagttg
     2821 ctcaacaaac tccatcatcc atatataatc cttgtatgcc acataaatct ccatacaggt
     2881 aaactcggga ttgtgggttc tgtccatacc ttcattgcgg aaattttttg caaactcata
     2941 gacgcctgta aaacccccca caatcaaacg ctttaggtat agttcgttgg caattctgag
     3001 ataaagtgga atatcaagag cgttgtggtg agtgatgaag ggtctggcag ctgctccacc
     3061 gggaattggt tgaaggatgg gtgtctcaac ctcaagatat ccatgctcgt tgaaaaagtc
     3121 cctcattgta gagacaattt tggtcctctt gacaaaggtt tccctcacct gaggattgac
     3181 gatcaggtca acatatctca tcctgtaacg aagtnnnnnn nnnncatata actctccctc
     3241 tttctctttt acgataggaa gtacacgaag agacttgcta agcaatgtca gtgactttgc
     3301 atgaatagat agttgacccg tctgggtaat gaatgcataa cctttgatac caataatatc
     3361 acctatatcc aaaagtttcc tgaaaaccgt attgtaaaga gtcttatcct caccctcaca
     3421 gatttcgtct cgtttgatat agacctgaat cttgccacac tcatcctgaa gctctacaaa
     3481 tgaagcagca cccataatgc ggcgactcat tatcctaccg gcaatggaga tatcattgaa
     3541 attggctttt tctgcatcat atccctcttt tatctctctc gaagtggcat ttatgtggaa
     3601 ttcctcagca gggtacgggt caatacctaa atccttcaat tgttgcagag cagcgcgacg
     3661 attcaattcc tgttcattga gatctgttat ttccatattg taaatctgac agttaagtat
     3721 tccttttgtt atatctggca aaaataagaa tatttatgga taaaggtaca aaaattgttt
     3781 atctttgtag aattgcagaa gtcaatatga gcattgaaaa aaaatggata gtgatggagc
     3841 cgggtaatcc ggctcttgta cgccaactga cacaggagct gggtattgat tctgtattag
     3901 ccaaccttct ggtccaaaga ggtatcgata cttttgcgaa agccagggag tttttccgcc
     3961 cggacctttc aatgctccac gatcccttct taatgaagga tatggataaa gctgtagagc
     4021 gtctccgcaa ggcagtagtc aagagagaaa ctatcttgat atacggtgac tatgatgtag
     4081 acggtacgac tgcggttgct ttggtctatt catttatgaa aaacctcaca aaccgggtag
     4141 aatactatat tcctgacagg tatgacgagg gatacggtat ttcttacaag ggcatcgata
     4201 ctgctagaga tcacaactgt accctgatca ttgcactgga ctgtggcatt aaagctattg
     4261 aaaaggttaa ctatgctaag agtctgggga tagatatgat tatctgcgac catcacttac
     4321 cggatgagaa actccccgat gccgtcgccg tacttgaccc aaaaagacag gactgcaact
     4381 accccttcga tgacctttcc ggctgtggag tgggctttaa acttgttcag ggctacgccc
     4441 aacaatatga tatccccttt gacaatatag tttcgttgct ggatcttctg gtggtcagta
     4501 tcgcatcaga cctggtctcc gtcacgggag aaaaccgtgt gttggcatac tatggactca
     4561 agcaactcaa tgaaaaccct cgtgaggggc tacagacgat aattaaactc tccggactgg
     4621 aaaaacaccg cattaccata gacgaaatag tctttaaaat aggccccaga atcaatgctg
     4681 cgggcaggat ggagtcgggt aaaactgcgg tcgatatgct tacttccaga gatgaagatg
     4741 aatcaaagga gattggggca gccattaatg cacataataa cgaccgcaag aaagtcgacc
     4801 gggagattac cgaagaggcg ctaaagatgg tgaaaagcat ccccggtttt gagaataaga
     4861 agtcaactgt ggtgtacaac ccatcctggc acaagggggt ggtaggtatt gtggcatccc
     4921 gtctggtgga ggcctactat agacctacca tagtacttac ccagtcaaat ggattaatca
     4981 caggctctgc ccgctccatt cagggttttg atctctatga ggccattgag tcctgttccg
     5041 acctgctgga gaactttgga ggacacatgt atgctgctgg gttaaccctc aaggaggaaa
     5101 atcttcagaa atttaccgag cgttttgaaa cctatgtagc ctcgaaaatc gacgatgtaa
     5161 tcctaacacc gattataaat atcgactcat atctggattt taagcagatt acacccaaat
     5221 ttttcagact gctcaagcaa ttccaacctt tcggaccggg caacctatct cctgttttta
     5281 ttacagagaa tgtctacgac aacggtaatg gccgcagagt gggatctgcc gaggcgggta
     5341 tgaaacacct caaattggaa ctcattcagg aggatgaacc ctacagatac atctctgcta
     5401 tcgcattcaa cctttctgat aactttgagc atatccaggg tggtaatccc gtcgatatct
     5461 gttattccat tgcagagaat tactatcgtg gcatcgccaa catccagctt cgcgtaaaag
     5521 atataaaaga aaaagagctg ttctaaactc tcactaatta gacagatgag ataatctctg
     5581 tcacacaagc aaagttgact ataataattg cacctactcc ctacacgata aacattttat
     5641 aatataagag ctatgaaaaa gttattatcc ctcattttat tgtcacttat ggtggcatca
     5701 tgcgaccctt tggaagaact aacaattgac tttgaactta aaggtaaatg gcttggtgga
     5761 ataagatatg aaataaagac tgagatagac gttcttgcaa aaagcctcca ttttaacgag
     5821 aactctaaaa gctgtactgt tgttacgggc ttatctttct taaatactat tgatgtagaa
     5881 gaccttgtag taagacccaa cggtgaaaac caactgattc tcaccaaaag aggcaataaa
     5941 gagcctcttt acaggcttta tttcacaaag gggaacaagg attcattcga gatgaagtgg
     6001 gaaaaccaca ccaaccttga gaaagaatac attccggaca aatcattttc cgtcataatg
     6061 aagccataca cctaaatctc ttaaacaaaa tttcatctac actatgtaga gatgaaatca
     6121 tcataaagat acaattatga aaaaaatagt attactggca ctactatcaa taattgtggt
     6181 atcctgtgat ttaattggag gaataaataa aaaatatgat ctcaacggta actgggccgg
     6241 agaaatcaaa tatgagataa tgtccgaaaa tgactaactt gtaaaaagtc tgattttctc
     6301 ggaagatgcc ggcaaatgta ctgtttatac cggcctctcg ttcttaaata ctgttgatga
     6361 agagagtctt atagtaaggc ctaatggtga taaccaactt attattaatg cgaaggagaa
     6421 caacaatgcg atatattctc tttattttac agagggcaat aaagattcat tcgaaatgaa
     6481 atggggaaac cacactaata tagaaaaaaa gtatattccc gataaatccc tctctgtcaa
     6541 gatgaagctg cttaatatcg actggaatta gttcgagaga tttctatttt tgaagtaaga
     6601 ggcgctttct gatgaaagtt aatgaaatac aaaagccccg acaactgatg ctgtcggggc
     6661 tttcttaaag ttggcggct
//
LOCUS       DAOSIE010000058         6597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_10583_length_6597_cov_4.022776, whole genome shotgun sequence.
ACCESSION   DAOSIE010000058 DAOSIE010000000
VERSION     DAOSIE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10583_length_6597_cov_4.022776"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLI69_02875"
     CDS             complement(<1..697)
                     /locus_tag="PLI69_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG41465.1"
                     /translation="MIPQYDIIVIGAGHAGCEAAIAAARMGSKVLLITKDMNKIAQMS
                     CNPAIGGIAKGQIVCEIDALGGYSAIVTDASTIQFRMLNLSKGPAMWSPRAQCDKLHF
                     SMNWRYVLENTSNLDIWQDTVTSITFKESNISGVRVAMGAEFDCKIVILTAGTFLNGK
                     LYIGNEVVHGGRMGESPSLALTEQLSALGCTTARMKTGTPPRIDARSIDLSKLEKQDF
                     DPNPSKFSYLPFKT"
     gene            complement(701..1147)
                     /gene="ybeY"
                     /locus_tag="PLI69_02880"
     CDS             complement(701..1147)
                     /gene="ybeY"
                     /locus_tag="PLI69_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOG41466.1"
                     /translation="MISFFNPDIDFNLPRKRILSKWLKEILLERGFHVGQINYIFCSD
                     DYLININRDFLGHDHYTDVITFDYSSDFEIQYGPNSISGDIYISIDTVRRNAELYDEG
                     FHKELHRVMVHGILHLIGFDDTSVELKAEMTQQEDSALQLLSLNKM"
     gene            1621..2313
                     /locus_tag="PLI69_02885"
     CDS             1621..2313
                     /locus_tag="PLI69_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOG41467.1"
                     /translation="MEVKKTPKADLENKKLLFREIGLIIALGLVLLAFEWSSTEKAET
                     VNLAVQEQVVEEEIIPVTQQEQLPPPEAPKIPVLSDIIDIVDDDIVVTDNLFIDMEDD
                     ASIGVEIMDYVAVVEEEEVEEEAIPFALVEEKPKFQGGDANDFSKWVNSKLVYPELAK
                     ENGVQGRVTLQFTVYTDGSVRDVKVLRGVDASLDKEAVRIVSMSPKWTPGKQRERPVK
                     VTYTFPVLFQLR"
     gene            complement(2423..3622)
                     /gene="hflX"
                     /locus_tag="PLI69_02890"
     CDS             complement(2423..3622)
                     /gene="hflX"
                     /locus_tag="PLI69_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOG41468.1"
                     /translation="MIKKDQIIKSSSDEMAAFVAIIPQGVEEREVNEYLDELEFLAET
                     AGVKGGKRFTQKLEKPNSRSYIGRGKLDEITAYIEQNEIDYLIFDDELSPSQLRNIEQ
                     AVGIRVIDRTGLILDIFAQRAKTAYAKTQVELAQYQYLLPRLTGMWTHLERQRGGISM
                     RGPGETQLETDRRIVLDKIAKLKEQLKKIDRQMASQRGNRGSLVRISLVGYTNVGKST
                     IMNLLAKSDVFAEDKLFATLDTTVRKVVIENVPFLLSDTVGFIRKLPTQLVESFKSTL
                     DEVREADILMHVVDISHLNFEEHIRVVNQTLLDIGAKEKPLFVVFNKIDAYRHEVYDE
                     FSLQEKKLNNYTLEELKESWMSKEHTPCIFISAKDKTNVDKLRSDLYNMVAEIHAGRY
                     PFDTFLW"
     gene            3689..4828
                     /locus_tag="PLI69_02895"
     CDS             3689..4828
                     /locus_tag="PLI69_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOG41469.1"
                     /translation="MKRLVLITTLSLISISLFSQGRIRDYGIEPGIFRTGEHNAITDV
                     PGVKVGQITIIKGDNIRTGVTAIIPHEGNLFRLKCPAAVYAGNGFGKLAGVTQIEELG
                     NTESPIILTNTLSVAQGIEGIITYTLAQQGNESVGSVNAVVGETNDGGLNDIRGRHVT
                     AEHVLKAIEIASGGPVEEGSVGAGTGTICFGFKGGIGTSSRVLPKSLGGYTVGVLVQS
                     NYGGVLEVDGVPIGEMLGQYSFKKAVEEEKSGQDPQLNPDGSCMMVVITDAPVDARNL
                     KRIAKRAMMGLAKTGGIASNGSGDYVIALSVNKDNLISESTATYNPTLLHNDDMSALF
                     EATIEATAEALWNSLFMATDVTGIGGRTVKALPVEKVVKLMGKQK"
     gene            complement(4834..5643)
                     /locus_tag="PLI69_02900"
     CDS             complement(4834..5643)
                     /locus_tag="PLI69_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG41470.1"
                     /translation="MERFVTAGGIAVHILDSEKGEKCIILLHGYLETMYIWSEFADLF
                     SQQYRVIVIDIPGHGLTDYAPADQSGNRINTMDFCASVVKGVLDKCNVSKAVIGGHSL
                     GGYIALACCRLYPDTFEKLILFNSHPYEDPEEKALDRKREIDIIESGKLSVLASMSIP
                     KMYSSLNLRDMDEKIRETIELCETHDPMGIVASIKGMQLRPDGSSVLLDGSIPSMVIV
                     GDQDSFMPVPMIEKIRGSFPKVRFEELSGCGHNCFVERPYEVFERVLSFLG"
     gene            5750..>6597
                     /locus_tag="PLI69_02905"
     CDS             5750..>6597
                     /locus_tag="PLI69_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOG41471.1"
                     /translation="MTKLVSHVPEGPLEQKWSSHKNNIRIVSPANKRKLDVIVVGTGL
                     GGASAAASLGELGYNVKVFCISDSPRRAHSIAAQGGINAAKNYRNDNDSVYRLFHDTI
                     KSGDYRSREANVYRLAEVSNNIIDHCVAQGVPFGRDYSGLLDNRSFGGGQVSRTFYAR
                     GQTGQQLLLGAYASLNRQVAKGRVKSYPRYEMLDLVVIDGEARGIIARNLVTGKIERF
                     GAHAVVIATGGYGNCYFLSTYAMTCNGSAIWQCYKKGAFFANPSFAQIHPTCIPVEGD
                     QQSKLTL"
BASE COUNT         1961 a         1378 c         1442 g         1816 t
ORIGIN      
        1 ttgtcttaaa tggaaggtaa gagaacttgg aaggattagg atcaaaatcc tgtttctcta
       61 gtttagagag atctattgag cgggcatcta ttctgggcgg agtgccggtt ttcattctcg
      121 cagtagtaca accgagagct gatagttgct cggtcagagc cagagaaggg gactccccca
      181 tccttcctcc atgaactact tcatttccga tatagagttt gccatttaga aaggtacctg
      241 ctgttaaaat aacgatctta caatcgaatt ctgcacccat agccacgcgg acaccggaaa
      301 tattggactc cttgaaggtt atggaggtta cagtatcttg ccaaatatct aagtttgagg
      361 tattttcaag gacatagcgc cagttcatgg aaaagtgtag tttatcgcac tgtgcacgcg
      421 gactccacat ggcaggacct tttgaaaggt tgagcatcct gaattgaatg gttgaggcat
      481 cggtaacaat ggctgagtaa ccacccaaag catctatttc gcaaactatc tgtccttttg
      541 cgattccacc aatggccgga ttacaagaca tctgagcgat tttgttcatg tcctttgtaa
      601 ttaggaggac tttcgaaccc attcttgctg ctgctatggc agcctcgcat cctgcgtggc
      661 cggcgccgat cactattata tcgtattgag gaattatcat ttacatttta ttcaatgaga
      721 ggagttgaag ggcactatcc tcctgttgag tcatttctgc tttgagttcc acagatgtgt
      781 catcaaaacc tattagatgt agtattccat gaaccatgac ccggtggagc tccttgtgga
      841 atccctcatc ataaagttca gcgttacgcc tgaccgtatc tatgctaata taaatgtcgc
      901 cgcttatgga gttcggacca tattgtatct cgaagtctga actataatca aatgttataa
      961 cgtcagtata atgatcatgc ccgagaaagt ctctgttgat gttgatcaaa tagtcgtcac
     1021 tacaaaagat ataattgatt tgtccaacat ggaatcctct ctcgaggaga atctctttca
     1081 accatttgga aaggattcgc ttcctgggta aattaaagtc tatatctgga ttaaaaaaac
     1141 ttatcataaa ctttggttaa tgttagaaaa catgcgggta tggcgtattt tttgctacaa
     1201 aaatagtgaa atgagtatag aacagacaaa aaaataaaaa aaagtgttaa ttggaaatta
     1261 aaacgtttcc aagcattaaa aataaggttc ttgaaaatag caaatgaaaa agctaacgat
     1321 caggattaag tgacgaaagt agaatagtaa tgaaggtatg atgcaagttt ctgtcggcag
     1381 gcatgcaaat atgagaaata tgagaaaggt ttagaataga tgaaaaaagg gtgtatataa
     1441 aaaatactaa caattataaa aagtggcaaa aaaccctaat tgattgacta tcagatatat
     1501 aggaaaaata aaataaaatt atcaacaaaa aatttgttat aaatgatttt ttcttactaa
     1561 ttttaccgat tactaggtca aatggaccaa aacttggaca aacaaaaata aaatactgat
     1621 atggaagtta aaaaaacacc caaagcagat cttgaaaaca aaaaattatt gttcagagag
     1681 attggactga taatcgcact cggattggta ttgcttgcgt ttgaatggtc atcaactgag
     1741 aaggctgaaa ccgttaacct cgcagttcag gagcaagttg ttgaggagga gattattcct
     1801 gtaacccaac aagaacagct tccacctcct gaggctccca agattcctgt tctgtccgac
     1861 attatcgaca tcgtagacga cgacatcgtt gtaaccgaca atctatttat agatatggag
     1921 gacgatgcga gtatcggagt ggaaattatg gactatgtag cagttgtgga ggaagaagaa
     1981 gttgaagaag aggccattcc ttttgctctt gtagaggaga aacccaagtt ccaaggtggc
     2041 gacgccaatg atttctccaa atgggtaaac agtaaacttg tttatcctga gcttgccaag
     2101 gaaaatggcg ttcagggtcg tgttacactg caatttacag tttacacaga tggatctgta
     2161 agagacgtta aagtactcag gggcgttgat gcttcgcttg acaaagaggc tgtaaggatc
     2221 gtctccatgt caccgaagtg gacacccggc aagcagagag aaaggccggt aaaggttact
     2281 tataccttcc cggtattatt ccaactccgc taaccgaagc tacagatcag taaatgttga
     2341 ggccgaccat tcaaagttgg cctcattttt tattttatat acttgttttg agtaaaaatc
     2401 tcgagaaaaa agttgtggaa aattaccaaa gaaaagtgtc aaaaggatat cgccctgcat
     2461 gtatctccgc caccatattg taaaggtcgg aacgaagttt gtccacattg gttttatctt
     2521 ttgcggagat aaatatgcaa ggggtatgtt cctttgacat ccacgattct tttagttcct
     2581 cgagggtata attattgagt ttcttttcct gaagagaaaa ttcgtcatag acttcatgcc
     2641 tgtaggcgtc aattttattg aaaacaacaa ataagggttt ctccttggct cctatgtcga
     2701 gaagagtttg gttgaccacc ctgatatgct cttcgaaatt gaggtgtgaa atatccacga
     2761 catgcataag tatatccgct tctctgacct catctaaagt gcttttgaaa gattctacaa
     2821 gttgagtagg aagttttctg ataaaaccta ctgtatcact taaaagaaaa ggtacattct
     2881 ctatgaccac cttacggaca gtcgtgtcaa gagttgcaaa aagtttatct tcggcaaaaa
     2941 catcactttt ggctaaaagg ttcataatgg tactcttgcc aacattggta tatcctacca
     3001 gggaaatacg cactaaagag ccccggttgc ccctttgaga ggccatctga cgatcaattt
     3061 tcttaagctg ctccttaagt tttgcaatct tgtccaagac tatgcgtcgg tcggtctcca
     3121 gctgagtttc acctgggccc ctcatgctta taccgcctcg ctgacgctca aggtgggtcc
     3181 acataccggt caatctgggt aaaagatatt gatattgtgc cagttctacc tgcgttttag
     3241 cataagccgt ttttgctcgt tgcgcgaaaa tatccaatat gagaccagtg cggtcaatca
     3301 cccttatccc gaccgcctgc tctatatttc ttagttgaga aggggaaagt tcgtcatcaa
     3361 aaataagata atcaatctca ttctgttcaa tataagcagt tatttcgtcc aacttgcctc
     3421 ttcctatgta gctgcgggaa tttggttttt cgagcttttg ggtaaaacgc ttaccacctt
     3481 taactccagc cgtctctgca aggaactcca actcgtccag atattcattg acctctcttt
     3541 cttcaacacc ttgtggaatg atagcgacaa aagccgccat ttcatcactg ctcgacttta
     3601 tgatttgatc cttttttatc atcaattcaa aatttttaca aaagtaaaac tatatttgta
     3661 ttaatataag attaaaaaat tattcaatat gaaacgattg gtacttataa ctactctgtc
     3721 tctcatctcc atttctctat tttctcaagg cagaatcaga gattacggca tagaacccgg
     3781 tattttccgt acaggagaac acaatgcaat taccgacgtg cccggagtaa aagtaggaca
     3841 gataactatc attaagggag ataatatccg tacgggagtg acggccatca ttcctcacga
     3901 aggcaatcta ttccgtctta aatgccctgc tgccgtctat gcaggcaatg gtttcggaaa
     3961 acttgcagga gttactcaaa tagaagaact cggcaatacg gagtctccga ttatcctgac
     4021 caacactcta tccgtggctc aagggattga aggcataata acctacacgc ttgcacagca
     4081 aggaaatgaa tctgtgggct cggtaaatgc ggtagtgggc gagactaatg atggtgggct
     4141 caacgatata cgcggaaggc acgtaacagc agagcatgtg ctgaaggcaa tagagattgc
     4201 atcaggtggt cctgtggaag agggatccgt tggggcaggg acaggtacca tctgcttcgg
     4261 ttttaagggt gggattggta cctcttctcg cgtacttccg aaatctctgg gaggatatac
     4321 ggtaggagta ttggtacaat ccaactatgg cggcgtcctg gaagtagatg gagtccccat
     4381 tggagaaatg ttgggccaat atagctttaa aaaggcagta gaagaggaaa aatccggtca
     4441 agatccgcag cttaaccccg acggctcctg tatgatggtg gtcataactg atgctccggt
     4501 ggatgctcgc aaccttaaaa gaattgccaa aagggcgatg atggggcttg cgaagaccgg
     4561 aggtatagct tccaacggca gtggggacta tgttatagca ctttctgtca acaaggacaa
     4621 tctcatcagc gaatctactg cgacttacaa tcccacgctg ctgcacaacg acgatatgtc
     4681 agctctattt gaggctacta tcgaagctac cgccgaggcg ctatggaatt ctctcttcat
     4741 ggccaccgat gtaaccggga tagggggaag aaccgtaaag gccctaccgg tagagaaggt
     4801 ggtaaaactg atgggaaagc agaaataaga ctattatcct aaaaaggaaa gaactctttc
     4861 gaatacttcg taggggcgct ctacgaaaca attatgtcca catcctgaga gctcttcaaa
     4921 acgcactttt ggaaagctcc cgcgtatctt ttcgatcatt ggaacgggca tgaagctatc
     4981 ctgatctcct acaatcacca tggagggaat tgagccatcc aggagtactg aggagccatc
     5041 cggacgaagt tgcatccctt taatagaggc tactatgccc atagggtcgt gggtttcaca
     5101 aagctcaatc gtctcacgta ttttctcgtc catatcacgc aaattgaggc tactgtacat
     5161 tttggggatg gacattgagg ccagcactga gagttttccg ctctctataa tatctatttc
     5221 gcgtttacgg tcgagggcct tctcttcagg gtcctcataa ggatgggaat tgaaaagaat
     5281 cagcttttca aaggtatcgg gatagagacg gcaacaagcc agggctatat aaccaccaag
     5341 agagtggcct cctattaccg cttttgaaac attacattta tcaaggacac cctttacgac
     5401 agaggcacag aaatccattg tattgattct gttgcccgat tgatcggcag gcgcatagtc
     5461 tgtaaggccg tggcccggaa tatcaatcac aatcactcta tattgttgtg aaaaaaggtc
     5521 tgcaaattcg ctccatatat acatggtctc aagataacca tgtaacagaa ttatgcattt
     5581 ttcacctttt tccgaatcta gaatatggac ggcaatgcca cccgctgtaa caaatctttc
     5641 caaattgagc ctaaaaattg atttgcgggc aaagttataa ataaaatggt atctttgcgc
     5701 cttaaaatag ttttaaaaag atataaataa caatacatct gaatctataa tgacaaaatt
     5761 agtttctcat gttcccgagg gacccctcga gcaaaaatgg agttctcaca aaaacaacat
     5821 ccgtattgta agccctgcca ataaacgaaa actcgacgta atcgttgttg gtacaggtct
     5881 tggcggtgca tctgcggctg cctctttggg agagctcggc tataatgtta aagttttctg
     5941 tataagcgat tcaccacgca gagcacattc catcgcagct cagggcggta tcaatgctgc
     6001 caaaaattac cgaaacgaca atgactcggt ttatcgtctg ttccacgaca ccataaaaag
     6061 tggtgactac cgtagccgtg aagcaaatgt ttaccgcctc gcggaagtct ctaataacat
     6121 catcgaccat tgcgttgcac aaggtgttcc ttttggacgc gattacagcg gacttctcga
     6181 taaccgttca ttcggaggcg gacaggtttc ccgtacgttt tatgcacgtg gccaaaccgg
     6241 acagcaactc cttctcggcg cttatgcgtc tctcaaccga caagttgcca aaggtcgcgt
     6301 aaagtcatat cccagatacg aaatgcttga tctggtagta atagacggtg aagcgagagg
     6361 cattatagcc cgtaaccttg taacgggcaa aatcgaaaga ttcggtgcac atgctgtggt
     6421 tatcgccacc ggaggatatg gtaactgtta ttttctctcc acctacgcaa tgacctgtaa
     6481 tggttctgcg atttggcagt gttataagaa aggagcattc tttgcaaatc cgagctttgc
     6541 gcaaatccat cccacttgta ttcccgtaga aggagaccag caatccaagc tgaccct
//
LOCUS       DAOSIE010000059         6426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_10925_length_6426_cov_3.944279, whole genome shotgun sequence.
ACCESSION   DAOSIE010000059 DAOSIE010000000
VERSION     DAOSIE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10925_length_6426_cov_3.944279"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..968)
                     /gene="prmA"
                     /locus_tag="PLI69_02910"
     CDS             complement(102..968)
                     /gene="prmA"
                     /locus_tag="PLI69_02910"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOG41472.1"
                     /translation="MDYIAVHIEVTPFSEERAEIVMAEIEELGFESFSIEEPYLHAYI
                     PKEDFSESALKAILSAYATGEFRVSYTTELIMEQNWNALWESNFEPIVVNGLITVKAS
                     HHKNLPPTKYTITIDPKMSFGTGHHQTTHLMMEVLLELGDAIKGLQVLDMGCGTGILA
                     ILAAKMGAKAPIHAIDIDHVCFYSTKENSFINGVGEEIHVLCGDASLLQASKYDLILA
                     NINRNILLEDMKTYARSLRPGGKLFISGFYSEDIPILLEEASQHSLELVSSRERDRWS
                     MVILRSAIHKNN"
     gene            complement(971..1726)
                     /gene="tpiA"
                     /locus_tag="PLI69_02915"
     CDS             complement(971..1726)
                     /gene="tpiA"
                     /locus_tag="PLI69_02915"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOG41473.1"
                     /translation="MRKKIVAGNWKMNTTVAEGKALAQEIVAALGSVPSDVQLVIAPP
                     FTHLVTVAEAIKGTPIALSAQNCADQPQGAYTGEVAVAMLKDAGCTYTILGHSERREY
                     YGETSEKLVKKIRLALEAGINPIFCVGEKLEERNAGKHFEVVGAQIEEVLFKFNAENM
                     KNIVIAYEPVWAIGTGVTATSEQAQEIHAFIRKFLADRFGSEIAEGTSILYGGSCKPS
                     NARELFACKDIDGGLIGGAALKAEDFIGIATSY"
     gene            complement(1729..2800)
                     /gene="prfB"
                     /locus_tag="PLI69_02920"
     CDS             complement(join(1729..2736,2738..2800))
                     /gene="prfB"
                     /locus_tag="PLI69_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157754648.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOG41474.1"
                     /translation="MITTEQIKEYKQRVMALGRSLDIAAKREELSEKQLKTTAPDFWD
                     DPKSAEIFLKEVSGLRHWVESYNKIESQMTDLETLLEFGEDASDDIYAVAKSLQGAIE
                     SLELRNMLSEEGDNLGALLKINSGAGGTESNDWSSMLMRMYIRWGERNGYKVHISELL
                     EGDEAGIKSVTIEFEGDYAFGYLKSEIGVHRLVRISPFNAQGKRQTTFSSVFVYPLVD
                     DTIEIEINPSDLEWDTYRSSGAGGQNVNKVETGVRVKHIPTGIVVENTETRSQMDNRQ
                     NALRILKSHLYDMELKKRREKQAELEGQKKKIEWGSQIRSYVLHPYKMVKDLRTNYET
                     SDIQGVLDGDLNAFMKEFLMNF"
     gene            complement(2778..3233)
                     /locus_tag="PLI69_02925"
     CDS             complement(2778..3233)
                     /locus_tag="PLI69_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOG41475.1"
                     /translation="MERDEVLDRMKRLCSRGERCRSDILRKVIAYGITDPESVVEKLV
                     REGYIDDERYARAFARDKSSLQGWGKVKIRVDLQRKRIDESAITAALNEIDPIAADTK
                     LQKLALSKLRQLQNESDPNLRKAKFIRYLMGRGYTYDEIVVIYDNNRTD"
     gene            complement(3240..5564)
                     /locus_tag="PLI69_02930"
     CDS             complement(3240..5564)
                     /locus_tag="PLI69_02930"
                     /inference="COORDINATES: protein motif:HMM:NF039873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG41476.1"
                     /translation="MKRSLCIFLFLITLYFGAFAQRPKVGVVLCGGGAKGAAHVGVLK
                     VLEEYDIPIDYIAGTSMGAIVGGLYSIGYSAAELDSLVMVQDWDFVMSDKVPRKLVSF
                     EAKKYDDRYLLKIPFSFRRDRGDLADKRPVTRDRDIIGPPQLLRGQQESSFLSNIPMY
                     YVSGHNVYNLFTKYSVGYQDSIDFNNMPIPFACVAVDLVGKKEVVFRSGYFVDAIRAS
                     MAIPGFFAPVRMDGMVLVDGGMLNNYPVDVAKEMGADIIIGVKLGFDDNRTSEIDDIG
                     DLLNELLSLYMEDKTEKAIDMTDIVIAPSVKDFSTMSFDIPSLRTLIDNGEKAAREKS
                     QELIRLKAYLDEMEQKEQKSLIGPKEIRKTYPKAVHIDQDSIIIGKVSSTGLSERDAE
                     WLLKKSKLKPGAVITGRDIDEEISRFYNTGSFSSVTYLLKGTESPYHLEINFVKGLSS
                     QLGVGFRFDSEEVATVMLNVSANNLALYGSKASITAKLAYNAMFMADYSYAFRANLQF
                     NTSYTFRSSDLNIFNKGLRADNISFYSHIADLNFSTRRFVSGQTSFGMRFHSFNFRSV
                     LSSSDVPPIYDPDIGRSNFISAYAKFKLDRMDREHFPSSGFVLNSGFAYHFNWLKPDE
                     YPFSVANLHFSVVIPLFSELALIPTIYNRTLIGGNIPVAFMNLMGGYEEGRYMEQQIP
                     FLGFNYTYAFKNILTVGSLDARLRLGRSHYILATGSYAMDSEGITDVFAEPGIVGVRL
                     GYSYDSPIGPISFNLHWSNFTGRLGAYLSLGYSF"
     gene            complement(5569..>6426)
                     /gene="rlmN"
                     /locus_tag="PLI69_02935"
     CDS             complement(5569..>6426)
                     /gene="rlmN"
                     /locus_tag="PLI69_02935"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOG41477.1"
                     /translation="IGHTLYTEKYISSDGTKKYIFPTSIAGRYIETVMIPEYEVNADM
                     EMGRATICLSSQIGCRMNCSFCMTGRMGYHGQLSAAEMISQFMNIDEAQELTNAVFMG
                     MGEPLDNWDEVSKVLQILTSEWGFGWSPKRITLSTIGVLPKLKSFLDDFKCHLAVSLH
                     TPFDEERAALMPVQKAFKLEEVLALIRRYDFTGQRRVSFEYIMFSGVNDTKRHADALI
                     RMLSGLECRINLIRFHSIPDSPLQPTPRTRIDDFKARLNNAGIITTLRASRGEDIMAA
                     CGLLAGQKR"
BASE COUNT         1742 a         1582 c         1291 g         1811 t
ORIGIN      
        1 taccggctca gaccacttcc acaacatcta tttcaacaat ttcaactata ccataggatt
       61 tctggccaag acagtaacaa ccaatggagt tcccggtgga gtcaattatt tttatgaata
      121 gcagagcgta atataaccat gctccatctg tcacgttccc gactcgaaac caactcaaga
      181 gaatgttgcg aggcctcttc caggagtatc ggtatatctt ctgaataaaa gccgctgata
      241 aagagtttac ctccgggacg caaagatctg gcataggtct tcatatcttc aagaagaata
      301 ttacgattga tattggctaa aatcagatcg tattttgagg cctgcagtag tgatgcatcg
      361 ccgcaaagta catgaatctc ctcccccact ccattaatga atgaattctc ttttgtggaa
      421 taaaagcata catgatctat atcgattgca tgaattgggg cttttgcccc catttttgct
      481 gccaggatag ccaatatccc ggtaccacaa cccatatcca acacctgaag tcccttgatg
      541 gcatccccaa gctccagcag tacctccatc ataagatgag tggtttggtg gtggcctgtg
      601 ccgaaggaca tcttcggatc aattgtgatg gtatattttg ttgggggaag atttttgtgg
      661 tgtgaagcct tgacggtgat tagtccattg acaacaatgg gttcgaaatt ggattcccaa
      721 agcgcattcc agttttgctc cataataagt tcggtagtgt aggagactct gaactcgccg
      781 gtagcatagg cgctaagaat tgctttcaga gcactctctg aaaaatcttc tttgggtata
      841 tatgcatgga ggtagggttc ttcgattgag aaactctcaa atccgagttc ttcaatctcc
      901 gccatcacta tctcagccct ctcttcacta aaaggggtca cctcgatatg gactgcaatg
      961 tagtccatga tcaatagctg gtggcgatac ctataaaatc ttccgctttg agagcagctc
     1021 ctcctatgag tccgccatct atatctttgc aagcaaagag ctcacgggca tttgagggtt
     1081 tacaactgcc tccgtagagg atggatgtcc cctctgcaat ctcactgcca aagcggtctg
     1141 cgagaaattt acggataaat gcgtggatct cctgggcctg ttcggaagtg gcagtaacac
     1201 ctgtgcctat tgcccatacg ggttcatagg caattacgat gtttttcata ttctcagcat
     1261 taaacttaaa aagcacctct tcgatttgcg ctccaacaac ctcaaagtgt ttaccggcat
     1321 tgcgctcttc aagtttttca cctacacaaa aaatagggtt tataccggcc tcaagtgcaa
     1381 gacgaatttt ctttaccaac ttctccgagg tttcaccata atattctctc ctttcggaat
     1441 gaccgaggat agtgtaggta caacctgcat cttttagcat cgctactgcc acttcgcctg
     1501 tataggctcc ttgaggctgg tctgcacagt tttgcgccga aagcgctatg ggagtccctt
     1561 tgattgcctc tgcaactgtc accaaatggg tgaaaggggg tgcgattaca agttgcacat
     1621 ctgagggtac gctacccaga gctgcaacaa tctcctgtgc gagagctttt ccttctgcaa
     1681 ctgtggtgtt catcttccag ttgcctgcta caattttttt tctcattatt aaaaattcat
     1741 taaaaattcc ttcataaatg cattaagatc accatcaagg acaccctgta tatcggatgt
     1801 ctcataattg gtgcgaagat ccttgaccat cttgtatgga tggaggacat aactgcgaat
     1861 ctgcgaaccc cattctattt tctttttctg tccctcgagt tcggcctgct tttcccgacg
     1921 ttttttgagt tccatatcgt agagatggga ttttagtata cgaagagcat tctgacgatt
     1981 gtccatctgc gaacgtgtct cagtattctc tacaacaatc cccgtcggta tatgctttac
     2041 tctgactccg gtctcaactt tatttacatt ctgtccacct gctccggaag agcgataagt
     2101 atcccactca aggtcggaag gattgatctc aatctctatg gtatcatcca ccaatgggta
     2161 aacaaatacc gaagagaatg tagtttggcg tttgccttga gcattgaaag gggatatcct
     2221 tacaagacgg tgaactccga tctcgctctt gagatatccg aatgcatagt ctccttcaaa
     2281 ctcgatggtg acagacttga tacctgcctc atcaccttcc agaagctctg aaatatggac
     2341 tttgtatccg ttgcgttcgc cccatcggat atacatgcgc atcagcattg aggaccaatc
     2401 attgctttct gtgccaccgg caccggaatt gatcttaagt agagctccca gattatcgcc
     2461 ctcttcacta agcatattac gcaattccag cgactctatt gcaccctgca acgactttgc
     2521 tactgcgtag atgtcatctg aggcatcttc gccaaactca aggagagtct cgagatcggt
     2581 catctgagac tcgattttat tataactctc tacccaatgc cgaaggcctg agacctcttt
     2641 tagaaaaatt tcggcagatt ttgggtcatc ccaaaaatcg ggagcggtag tcttcaattg
     2701 tttttccgaa agttcttcac gctttgcagc tatgtcaaag agacctcccc aacgccatta
     2761 cacgctgttt atactcttta atctgttctg ttgttatcat aaatcactac tatttcatca
     2821 taagtatagc cgcggcccat aaggtaacgg atgaattttg ccttacgcag attggggtca
     2881 ctctcatttt gcagttgtct gagttttgaa agggcaagtt tttggagttt tgtatccgcc
     2941 gcaatggggt caatctcatt tagcgcagct gtaatggccg actcgtcaat ccttttacgc
     3001 tgcaaatcta ctcttatttt tacttttccc caaccttgca aagaactttt atctctcgca
     3061 aaggccctcg catacctttc atcatctata taaccttctc tgaccagctt ctccaccacc
     3121 gactcaggat ccgtaatccc ataagctatc actttacgca gtatatcgct gcgacaacgc
     3181 tcaccacgac tacagagcct cttcatccta tctaagacct catctctctc cataccgtat
     3241 cagaatgaat atcccaaact aagataggcc cccaacctcc ccgtgaaatt tgaccagtgc
     3301 agattgaaag aaatcggacc tatcggggag tcgtaggaat aacccaaacg gactcctaca
     3361 ataccgggct ctgcaaacac atctgtgata ccttcactgt ccatcgcata actgccggta
     3421 gctaatatgt agtgcgaacg gccaagacgc aaccgtgcat ccaaagagcc gactgtaagg
     3481 atgtttttga aggcgtatgt atagttaaat cccaaaaatg gtatctgttg ctccatatat
     3541 cgaccctcct cgtaaccccc catcaggttc ataaaggcta cgggaatgtt accaccgata
     3601 agagtgcgat tgtaaattgt cggaatcaac gccagttcgc taaataaggg gattacaacg
     3661 gaaaaatgca gattggccac tgagaaggga tattcatcag gcttaagcca gttgaaatgg
     3721 taagcaaacc cggaatttag aacaaagccg ctgcttggga aatgttccct atccatcctg
     3781 tcgagtttga attttgcgta agcactgatg aaatttgatc ggcctatatc ggggtcataa
     3841 attggcggca catccgatga ggagagcacc gacctgaaat tgaaggaatg aaacctcatt
     3901 ccaaacgagg tctgtccact gacaaacctc ctggtcgaga aattgaggtc tgcaatatga
     3961 ctgtagaatg agatattgtc agccctcagt cctttgttga atatattgag gtccgatgat
     4021 ctgaaagtgt aggaagtgtt gaactgcaaa ttggccctga atgcatagga gtagtctgcc
     4081 ataaacattg cattgtatgc caattttgcc gttatggagg ccttggagcc atacagtgcc
     4141 agattatttg cgcttacatt gagcatcacc gtagctactt cctccgagtc aaacctaaat
     4201 cctacaccca gctgagagct taaacctttg acaaaattga tttccagatg atagggactc
     4261 tcagtccctt taagcagata ggtaaccgat gaaaatgatc ccgtattgta aaaccttgaa
     4321 atctcttcat caatatcacg accggtaatc accgctccgg gctttaattt actctttttc
     4381 aaaagccact ctgcatctct ttccgaaaga ccggtcgaag agacctttcc aattattata
     4441 gagtcctggt ctatatgtac cgccttaggg taagttttcc taatttcttt aggaccaata
     4501 agacttttct gttccttttg ttccatctcg tcgagatatg ccttaagacg tatcagttcc
     4561 tggcttttct ccctggcagc cttttcacca ttatcaatca aagtacggag actcggtata
     4621 tcgaaactca tagtggagaa atcttttaca cttggagcaa ttacaatatc cgtcatatcg
     4681 atagccttct ctgttttgtc ttccatatag agagaaagaa gctcattgag caaatctcct
     4741 atatcgtcta tttcagaggt gcggttatca tcaaagccga gctttactcc aattattata
     4801 tccgccccca tctcttttgc tacatcaaca ggataattgt tgagcattcc accatccacc
     4861 agcaccatac catccattct cacgggggca aagaatcccg gaatagccat tgatgccctt
     4921 attgcatcca caaaataacc gctacggaag actacctcct ttttgccaac caaatccaca
     4981 gccacacatg cgaaaggtat cggcatattg ttaaagtcaa tagagtcttg gtaacccact
     5041 gaatactttg taaaaagatt gtaaacgttg tgaccgctga cataatacat aggaatattg
     5101 gagagaaaag agctctcctg ctgccctcga agtagctgag gaggtccgat tatgtcgcga
     5161 tctctagtta caggcctctt gtctgccaga tcacccctat cccttctgaa agaaaatgga
     5221 attttgagca agtagcgatc gtcatacttt ttggcctcaa aggagaccag cttgcggggg
     5281 actttatcac tcattacaaa atcccaatcc tgtaccatta ctagagaatc caactcagcg
     5341 gcagagtaac ctatggaata gaggcctccc acaatggcac ccatactggt accggcaata
     5401 tagtcgatag gaatatcgta ctcttcaagc accttgagta ctccaacatg cgcggctccc
     5461 tttgctccac ccccacacaa cactacacct actttaggtc tttgtgcaaa agcgccgaag
     5521 tagagtgtaa taagaaatag aaatatgcat aaggagcgtt tcataatctt atcttttttg
     5581 tcctgcaaga agtccgcaag ctgccattat atcttcaccg cgtgaggcac gcagggtagt
     5641 tataatgccg gcattgttga gacgagcctt aaagtcatct atcctggtgc gcggagtggg
     5701 ctgaagcggt gagtccggaa tagaatgaaa tctaataagg ttgatacgac attccagtcc
     5761 ggaaagcatt cttatcaaag catcagcgtg acgtttagta tcgttgaccc cggaaaacat
     5821 tatatactca aaactcaccc ttcgctgacc ggtaaaatca tagcgacgta tgagtgctag
     5881 tacctcttcc agcttgaatg ccttctgcac cggcataagc gctgccctct cttcatcaaa
     5941 tggtgtatgc aaactaacag ccaaatggca cttgaagtca tccagaaaac tttttaactt
     6001 tggcaatact cctatggtag aaagggtgat gcgcttagga ctccatccaa acccccactc
     6061 cgaggtaagt atctgcagca ctttagatac ctcatcccag ttatccaacg gctcacccat
     6121 ccccataaaa acggcattgg taagctcctg cgcctcatct atattcataa attgggaaat
     6181 catctcagct gccgagagct ggccgtggta gcccattcga ccggtcatac agaaagagca
     6241 attcatcctg cacccaatct gcgaagagag acatattgta gctcttccca tttccatatc
     6301 ggcattaact tcatactccg gtatcatcac cgtctcaatg tatcttcctg caattgaggt
     6361 tgggaaaata tattttttgg taccgtcgga ggagatatat ttttccgtat aaagcgtatg
     6421 acctat
//
LOCUS       DAOSIE010000060         6378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_11024_length_6378_cov_4.005852, whole genome shotgun sequence.
ACCESSION   DAOSIE010000060 DAOSIE010000000
VERSION     DAOSIE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11024_length_6378_cov_4.005852"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLI69_02940"
     CDS             complement(<1..223)
                     /locus_tag="PLI69_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41478.1"
                     /translation="MSTFNLTYPVGWDKICKAVNMAYGYYDNVEVLVDNEVIDISSAE
                     EILTLDEAGTMIIRGISTIIKAPVMITFFN"
     gene            complement(262..1062)
                     /locus_tag="PLI69_02945"
     CDS             complement(262..1062)
                     /locus_tag="PLI69_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41479.1"
                     /translation="MTLALLSSLGCTNAQKKELVSVSYHRSGMRSDDCTDIRVNKTGD
                     TYTLVFEKGRNEANTISFEIEKSKFDELATIVFGMSKPRKERRIYVRDLMESLQVSFI
                     KNGKTIYYNYSINQRKDKKTAELEERAIDLMYDWIDRYKKQFEIRFNYSKGIATPTAI
                     YTHAEPADLVKDLGEFIERLDPNRDEQCGGTNYYSHRWRALKPGVVTVWLYELSLGHD
                     DVSNFPKDYEPTACYIIDKNLHVRYSKEETEAAQERFKNRKKYNRRTE"
     gene            complement(1130..1666)
                     /locus_tag="PLI69_02950"
     CDS             complement(1130..1666)
                     /locus_tag="PLI69_02950"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HOG41480.1"
                     /translation="MGLKLKMTKYQTITHSLRPLFRADSKILILGSFPSVKTREYGFF
                     YGHPQNRFWPLLERIFGVKLSTDIEERRAFLLQHHLALYDVIYRCDIIGSSDASIQNA
                     VPSDLSPILKEADIKQIFCNGGTAYRYYKKYQEQQTGFKAIKLPSTSPANARSSMDDL
                     YQKWKIIKEYQLEIADTI"
     gene            complement(1916..1988)
                     /locus_tag="PLI69_02955"
     tRNA            complement(1916..1988)
                     /locus_tag="PLI69_02955"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1953..1955),aa:Lys,seq:ttt)
     gene            complement(2105..4147)
                     /locus_tag="PLI69_02960"
     CDS             complement(2105..4147)
                     /locus_tag="PLI69_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOG41481.1"
                     /translation="MRRLIALSIGIAALLISFSAEAAKEKKVTLNSPDNRTIITVTIG
                     EEITWSVIFQGTEVIAPSTLSMEIESADGRKSFIWGNDSRLRKVSRTTSDRIISSPIY
                     KSSSVREHYNQMELYFREDFGLIFRAYDEGVAYRFYGTRLKEDDKVKDELAEFNFNQD
                     RTAYIPYSTGNPNPYRTSFENRYEVKSLSSFDKEKIAFSPLLVCLDGDFKAVITESDL
                     ESYPGMFLEKSGEFSLRGRFAPIPSEIEVHPTRCQERPTAYSDILALIRNDSSKKSPR
                     YFPWRVIAISDKDTRLPENNLVWLLAPENRIEDISWIKPGKVAWDWWNNWGVTGVEFQ
                     VGINTATYKHYIDFTSANGIEYVILDEGWSPPKEGDIMKVIPEIDLEELVSYGRERNV
                     ELILWAVAYPLDKKLEQACSHYSAMGIKGFKVDFMDRDDQDVVELNHRIAEVAAKYRM
                     LINFHGMYKPAGLNRTWPNVINFEGVWGLEQMKWSKEDMVSYDVTFPYIRMLSGPVDY
                     TQGAMRNAIRRDYVPNYNNPMSQGTRARQVAEYIIFDSPLVMLCDNPTIYTKEQETTD
                     FITAIPTVWDETRVLQGELGKYIVTARRSGGTWYIGGMTDWEERDITLDVSFLLDGNK
                     SAGKGTITLFRDGPNAHRSAHDYTIETLNVVNYIARKSTSLTIHLAPGGGFAMVVE"
     gene            complement(4202..4576)
                     /locus_tag="PLI69_02965"
     CDS             complement(4202..4576)
                     /locus_tag="PLI69_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOG41482.1"
                     /translation="MGKGFITILLLVISNSFMTLAWYGHLKFGSESKLLSKWGLFGVI
                     VLSWLIAFFEYCAQVPANRIGFQGTGGPFSLMQLKVIQEVITLVVFVIFATIFFKNET
                     LKSNHLIAFIFLILAVYFVFKK"
     gene            complement(4584..5180)
                     /gene="pnuC"
                     /locus_tag="PLI69_02970"
     CDS             complement(4584..5180)
                     /gene="pnuC"
                     /locus_tag="PLI69_02970"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOG41483.1"
                     /translation="MDNFNLYFEIFAAVMGITYVTLEVLQKRSMWVMLFITSICYGIV
                     YYITGLYAMMALQFYYIYMAISTFIVWGRNSRAGEKANQSSTGIEIRKLNPRVALASI
                     GVSAVAFVALGFIFGLTDNPRPWIDSAAAVLSMLATYWLSKKYIEQWYVWIVCNAISI
                     WLYISQGMWPTMILFVAYTIMSITGIFLWRKKGVVISD"
     gene            5333..>6378
                     /locus_tag="PLI69_02975"
     CDS             5333..>6378
                     /locus_tag="PLI69_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOG41484.1"
                     /translation="MAREIKFSLLYRDMWQSSGKYVPKVDQLNQIAPVIIEMGCFDRI
                     ETNGGAFEQVNLLFGENPNRAVREWTAPFNKAGIQTHMLERGLNALRMNPVPNDVREL
                     MYKVKIKQGTNIARSFCGLNDHRNLKGSVLGAKKAGMISQVALSITYSPVHTVEYYMG
                     IVDKVVGYGCDEICLKDMAGIGRPVFLGRLVAEIKKKYPNLLVQYHGHSGPGFSVASM
                     LEVARAGAEYIDVAMEPLAWGKIHPDVITIQAMLKDAGFLVKEINMDAYMEARHLTQT
                     FIDDFLGYFIEPTNYQSSSLLVGCGLPGGMMGSMMADLQGFLGAINMSLKNQGKKEVT
                     LDELTVMLFQEVEYV"
BASE COUNT         1736 a         1385 c         1393 g         1864 t
ORIGIN      
        1 gattaaaaaa cgttatcata accggggctt tgattattgt cgatatcccg cgaattatca
       61 ttgtcccggc ttcatcgagt gtcaaaatct cctctgctga ggaaatgtca attacttcgt
      121 tatcgactaa aacctctaca ttatcgtaat aaccgtaagc catatttaca gctttacaga
      181 tcttgtccca tccgaccgga taggtcaggt taaatgttga cataagccaa cctcgttctt
      241 ctcctacacc taataaaagc attactctgt tctcctgtta tattttttgc ggtttttaaa
      301 tctttcctgt gccgcttccg tttcctcttt agaatagcgc acatgcaggt ttttgtctat
      361 gatgtagcag gcggtgggtt catagtcttt tgggaaatta ctaacgtcgt cgtgtcctaa
      421 gctcaattca tagagccaaa cggtaactac accgggtttt aaggcacgcc agcgatggga
      481 atagtaattt gttccgccac attgctcgtc acggttcggg tcaagacgct ctataaattc
      541 accgagatcc ttcaccaagt cagcaggttc tgcgtgggta taaattgccg taggcgttgc
      601 aataccttta gagtaattaa agcgaatttc gaattgtttt ttgtacctgt caatccaatc
      661 atacataaga tcaatagccc gctcctccaa ttcagcagtt tttttgtctt tgcgttgatt
      721 gatgctataa ttataataaa tggttttacc gtttttaata aatgatacct gaagggattc
      781 catcaagtcg cggacataga tacggcgctc tttgcgcggt ttgctcatcc cgaaaacaat
      841 agttgccaac tcatcaaatt tgcttttctc aatctcaaaa ctaatcgtat tagcttcatt
      901 tcgacctttt tcaaatacca gggtataggt gtccccggtt ttattaaccc taatatcggt
      961 acagtcatcg ctgcgcatac ctgaacggtg ataagatact gagacaagtt cttttttctg
     1021 tgcgtttgtg catccgagag aggagaggag tgctaatgtc ataataatca gttttgtttt
     1081 cattcacacg ttattgtttt acctatttat cattaactaa ttctaatgat tatatcgtat
     1141 ctgctatttc taattgatat tctttaatta ttttccattt ttgatacaaa tcatccatag
     1201 aagatcttgc gttggcagga cttgtagagg gcagtttaat ggctttaaat ccggtttgct
     1261 gttcctgata ctttttataa tagcgataag cagtaccgcc gttgcagaaa atttgcttta
     1321 tatccgcttc ttttaaaatt ggacttaaat ccgagggaac tgcgttttga atgctcgcat
     1381 cacttgagcc aatgatatca caacggtaga tgacatcgta gagggcgaga tggtgttgta
     1441 atagaaaagc tcttctttct tcaatatcgg tgctaagttt cacaccaaaa atccgttcaa
     1501 gcaaaggcca aaaacgattc tgtggatgcc cataaaagaa accatactct ctggttttga
     1561 cagaggggaa agaacccaaa atcaaaattt tagaatcggc ccgaaaaaga ggccgaagtg
     1621 agtgagtaat ggtttgatat ttggtcattt ttaactttaa tcccatcttc agatttctgt
     1681 acaaaaataa gatttttgtg ttacattaac tactataaac ttactcgtaa cttgaaactc
     1741 ggaactcata actaaataga ggcgaccaat ggcagcattc taagtcagcg aagctgaagg
     1801 cagcgcttct gcgatgcatt ggttcctcaa gggaaccatt aaaaggaggg gtagacaagg
     1861 agcgcagcga ctgatcacaa aagactgagc gacctttggg tcgctcagtc ttttgtgatc
     1921 cgcctgggat tcgaacccag ggccccatcc ttaaaaggga tgtgctctac ctgctgagct
     1981 agcgaatcag gaggtgcaaa ggtatggata aaagtagtat tttgcaaaaa aaagtagttt
     2041 ttgctccaaa agtgcctcta agcgcattta tgtcgcctaa tctgaagcta cggcacagcc
     2101 tattttactc caccaccatt gcgaagccac cgcccggtgc cagatggatg gtaagagaag
     2161 tgctttttcg ggcaatataa ttgactacat tgagcgtttc tatggtgtag tcgtgtgctg
     2221 aacgatgtgc attgggacca tcccggaaga gtgttatagt gccttttccg gcgcttttgt
     2281 tgccgtccag gagaaaactc acgtcgaggg taatatccct ttcctcccaa tcggtcatac
     2341 ctcctatata ccatgttccg ccactgcgac gtgcggtaac tatatatttg cccagctctc
     2401 cctgaagtac cctcgtctca tcccaaacag ttggaatggc agtgatgaaa tcggtagttt
     2461 cctgctcttt ggtgtaaatt gtaggattgt cacagagcat taccagcggt gaatcaaata
     2521 ttatatactc cgctacctgg cgggcacggg ttccttggct catcggatta ttgtagttgg
     2581 gtacataatc acggcgaatg gcattgcgca tagctccttg agtatagtca accggaccgg
     2641 agagcatcct aatataaggg aaggtaacat cgtaggagac catatcttcc ttactccact
     2701 tcatctgctc aagtccccag actccctcga agttgatcac atttggccag gtacggttga
     2761 ggccggccgg cttgtacata ccatggaaat taatgagcat gcgatatttc gctgcaacct
     2821 ctgcaatgcg gtgattaagc tccacgacat cctgatcgtc cctatccata aagtccacct
     2881 tgaacccctt tatgcccatt gccgaatagt gactacaagc ctgttcgagc tttttatcga
     2941 gagggtaggc cactgcccag agaatcagtt caacattgcg ctcacggcca taactgacaa
     3001 gctcctcaag gtcaatttca ggaataacct tcataatatc accctctttt ggaggtgacc
     3061 atccctcgtc cagtatcaca tattcaatac cgttggcaga ggtgaaatcg atgtagtgct
     3121 tatatgtagc agtgtttatt cccacctgaa actccactcc ggtcactccc caattgttcc
     3181 accaatccca ggcaaccttg ccgggtttga tccaggaaat atcctctata cgattctcag
     3241 gggcgagcag ccataccaga ttgttttccg ggagccgggt atccttgtcg gaaattgcaa
     3301 tcactctcca gggaaaatac ctgggactct ttttgctgct atcgttgcgg attagcgcca
     3361 gaatatcaga ataggcggtg ggacgctcct gacatctggt gggatgcacc tctatttctg
     3421 aggggatagg ggcaaagcga cccctaagtg agaattctcc acttttttca aggaacatcc
     3481 cggggtaact ctcaaggtcc gattccgtaa tcacggcttt aaagtcgcca tctaggcata
     3541 ccagcagcgg tgaaaaagcg attttttctt tgtcaaagct actgagagac tttacttcgt
     3601 atctgttttc gaatgatgtc cggtaggggt tgggattacc tgtgctgtaa gggatgtatg
     3661 cggtgcggtc ttgattgaag ttaaactccg ccagttcgtc cttcacctta tcatcctctt
     3721 tgagtctggt gccgtaaaaa cggtaggcca ctccttcgtc ataagccctg aagatgagtc
     3781 cgaagtcctc gcggaagtag agttccatct ggttgtagtg ctctcttacg cttgaactct
     3841 tgtatatggg tgaggagatt atacgatcag atgtggtgcg gctcaccttt ctgagtctgc
     3901 tatcgttgcc ccagatgaag gattttctac catcggcact ctctatttcc atcgaaaggg
     3961 tagaaggcgc gatgacctct gtcccctgaa aaatcaccga ccaagtgatc tcttcgccaa
     4021 ttgtaacggt aataatggtg cgattatccg gggaattcag tgttactttt ttctcttttg
     4081 ccgcttccgc ggaaaatgaa atcaacagag ctgcaatgcc tattgataat gcaattaatc
     4141 gtctcatata atttttctta atgagacaaa aataaagaaa atagtgatga gagacaagat
     4201 gttatttctt gaaaacgaag tagaccgcca gtatcagaaa aataaatgca atcaggtggt
     4261 tggatttcaa tgtttcgttc ttgaagaaaa tagtagcgaa tataacaaat accacaaggg
     4321 tgatgacctc ctggatcacc ttaagctgca taagtgaaaa ggggccaccg gtgccttgaa
     4381 agccaattct gttggctggc acttgggcac agtattcaaa aaaagctatt aaccagctta
     4441 gaactataac tccaaagagt ccccacttgg agagcagctt actttcgcta ccaaatttta
     4501 ggtggccata ccaggccagc gtcatgaagc tgtttgaaat caccagtagc aatatagtaa
     4561 taaatccttt tcccatttct gcgttaatct gaaattacta ctcctttctt tctccacaaa
     4621 aatatgcctg ttattgacat tatagtgtat gcaacaaaaa gaatcattgt aggccacatt
     4681 ccctgcgaaa tgtagagcca gatggagatg gcattacata cgatccatac ataccattgt
     4741 tcaatatatt tcttggagag ccagtaggta gcgagcatgc tcagcacagc agctgctgaa
     4801 tctatccagg ggcggggatt gtctgtcagt ccgaatataa atccgagcgc cacaaaagcc
     4861 actgcactga caccgatgct tgccagggca acgcgtgggt tgagtttgcg tatttctatg
     4921 ccggtactac tttgatttgc cttttcccct gcacgggagt ttctacccca tacaataaat
     4981 gtacttattg ccatatatat gtaataaaac tgcagggcca tcattgcata aagtcctgtg
     5041 atataataaa ctataccgta acatattgat gtaatgaaaa gcataaccca catggagcgc
     5101 ttttgcagca cttcaagtgt cacgtatgtg atgcccatga cagcagcgaa aatttcaaag
     5161 tagaggttga agttatccat atattatttt tgaattgtcg ggcaaagata taaaaagttg
     5221 cgatttttac tttatgaaat attggattga agaaataatg attaatttcg taattcagtt
     5281 aaaattgaag ataatttagt tatatagtta tttaaattta tttaaaaaaa acatggctcg
     5341 tgaaatcaaa ttttctctgt tgtacagaga tatgtggcaa tcttcgggta agtatgttcc
     5401 caaggttgat cagttgaacc aaatagctcc tgtaataatt gaaatgggat gttttgaccg
     5461 catagagaca aatggcggtg cttttgagca ggtcaatctc cttttcggtg aaaacccaaa
     5521 ccgtgccgtg cgcgagtgga ctgccccctt caataaagcg ggtatccaga cccatatgct
     5581 tgagagaggt ctcaacgcac tacgaatgaa tccggttcca aatgatgtac gtgagcttat
     5641 gtacaaggtt aagataaaac agggtaccaa cattgcacgc tcattctgcg gtttgaacga
     5701 ccatcgtaac ctcaagggat ctgtactggg agcaaagaaa gcagggatga tttctcaggt
     5761 ggctttgagc attacttatt cacctgtaca tacggtagaa tattatatgg ggatcgtgga
     5821 caaggttgtg gggtatggtt gtgatgagat ttgcttaaaa gatatggctg gtatcggccg
     5881 tcccgtcttc ctcggtcgtc tcgttgcaga gataaagaaa aaatatccca atctactggt
     5941 acagtatcat ggccactccg gccccggctt ctccgtggcc tctatgcttg aagttgcacg
     6001 cgcaggtgcc gaatatatcg acgttgctat ggaacctctg gcctggggta agattcaccc
     6061 cgatgtaatc accattcagg cgatgcttaa ggatgcagga tttctggtta aggagataaa
     6121 tatggatgcc tacatggagg cacgtcatct tacgcagact ttcattgacg actttctcgg
     6181 ctacttcatt gagcccacca actaccagag ttcgtcgctg cttgtaggtt gcggtctgcc
     6241 cggcggtatg atgggatcaa tgatggctga tcttcagggt ttccttggtg ctataaatat
     6301 gtctttgaag aatcagggta agaaagaggt tacccttgac gaactgacgg taatgctctt
     6361 ccaggaggtt gaatatgt
//
LOCUS       DAOSIE010000061         6284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_11249_length_6284_cov_4.793867, whole genome shotgun sequence.
ACCESSION   DAOSIE010000061 DAOSIE010000000
VERSION     DAOSIE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11249_length_6284_cov_4.793867"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /gene="yidC"
                     /locus_tag="PLI69_02980"
     CDS             <1..1195
                     /gene="yidC"
                     /locus_tag="PLI69_02980"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOG41485.1"
                     /translation="NYSAIAYRFSGTNNVKSFGMRKDSAKESFSANLRWFGFQQQFFS
                     AILVAENNFVGGNLENKFYTEDVAIATGDLMRSSAEMVLDLDTQSKDFSLPFKFYFGP
                     NHYPTLKSYDEGFEKIIPLGGKLIGPINRYVIIPVFNWLSKYISSYGLIILILTILIK
                     IVISPLTLKSYKSSAKMKVLQPEIKQINERYPKQEDAMKKQQATMDLYKKAGVSMFGG
                     CLPMLLQFPILFAMFRFFPASFELRQQSFLWADDLSAYDSILDFGFNIPLYGDHISLF
                     ALLMGVSMFFYSRLTMSQMADNQQMPGMKFMQTWFMPIFMVLICNNFSAGLSYYYMLS
                     NLITIIQTLIIRKWFVDEKKLRAQIELRASSKEPQKKSKFRQRLDEAYRIQQEQMRQQ
                     QKRKR"
     gene            1192..1839
                     /locus_tag="PLI69_02985"
     CDS             1192..1839
                     /locus_tag="PLI69_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOG41486.1"
                     /translation="MTIRERIESILSKLPPTVKLVAVSKLHSVPEILEAYVAGQRAFG
                     ESRPQEMAAKVPMLPKDIEWHFIGPLQSNKIKMILPHVSLIHSVDSERLLARIDRQAA
                     RSGILVDCLIQLHIAIEDTKQGFPVDEALEQVPLFRDRYKNVRIRGLMGMASFVDDME
                     QVRGEFRTLKSAFDKLKPEFSLFDTLSMGMSQDWHIAVEEGATIVRIGSTIFGSR"
     gene            1904..2920
                     /locus_tag="PLI69_02990"
     CDS             1904..2920
                     /locus_tag="PLI69_02990"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOG41487.1"
                     /translation="MHNDLLGKDLRYGLIGSGSWATALVKVLSDTGNRVNWYIRNSDN
                     IDFIKEFGRNRFYLRSAALKTGMLEMSDDINEVVYNSDAVIFCIPSAYFLAQIKPLDN
                     DLLKDKLLISATKGFVSNDHLTVAEYFHRVLGIPFSRIVVLSGPTHAEEVAMEKLSYL
                     TFASKHIEVSSKVSEAFSSHYVKVVCSTDIYGVEYSAAMKNVYAVAVGLCHSLGYGDN
                     FISVLVTGAYNEMVRFLNSTHPDSSRIPSRSAYLGDLLVTCYSQFSRNRTFGGMIGKG
                     YSVVSAHTEMNMVAEGYYATRCMYEIANRLNVELPIVNAMYQILYEEKSPHYVVNHLA
                     SILK"
     gene            2946..4277
                     /locus_tag="PLI69_02995"
     CDS             2946..4277
                     /locus_tag="PLI69_02995"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOG41488.1"
                     /translation="MINLDLKGCSSFVNEKQFEDYTARALEALSILDSGTGAGSNFLG
                     WNRLPSEITQEMLEDYLSVVDKWIEMEIDLVVVIGIGGSYLGAKAAIEALSHSFHQMG
                     PSRGPKVVFAGHNLSEEYIAELMDMVSVKNVACVVISKSGTTTEPAVVFRLIKKYIEE
                     TYGIKEARKRIIAVTDKERGALRLLSNQEGYKTFVIEENIGGRFSVLTAVGLLPIALA
                     GFDITEMVRGAVDMQRICSEPTKDNPAIRYAAMRNILYDNGKKIELLVGFNPKLHYLS
                     EWWKQLFGESEGKDGKGIFPASVNFTTDLHSLGQYIQDGERILFETFIDVRTIRRKVE
                     IGYDKQDLDGLNYLAGKNVEFCNKMAQQGTMFAHNDGGVPTMEISIDLLDEYNLGQLF
                     YFFEKACGISAYILGVNPFDQPGVEDYKKNMFALLGKPGYERQSKELRKRL"
     gene            4240..5274
                     /locus_tag="PLI69_03000"
     CDS             4240..5274
                     /locus_tag="PLI69_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HOG41489.1"
                     /translation="MNDRVKSYVRDFDTFYIIMKTKYILMLFLAASLLVSCNTQEQKE
                     PEEWAIVLHAGAGGTQAMSVEDEASYCIFLNEALEQGKTILSSGGSALDAVEATVIYL
                     EDCPLFNAGKGAVMTSDGRHELDASIMDGRDFSAGAVAGVTDIKNPIKAARLVMEKTP
                     HVLLIGEGASFFAREQGLEIVPNSYFSTESSKIELERALAEKKSGSPIGTVGCVALDS
                     DGNIAAATSTGGMTGKKWGRVGDVPIIGASTIADNNFVGVSSTGHGELWIRRSVAHDI
                     YALMDYKGFSLEEAVHEVIWNKIDKMEGSGGGVICIDRFGNIVMDFNTHIMYRAWAKA
                     SGESGAAVNK"
     gene            5301..5792
                     /locus_tag="PLI69_03005"
     CDS             5301..5792
                     /locus_tag="PLI69_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOG41490.1"
                     /translation="MKKLINYTIVLLAAISLAGCGTPKEAPIDLNGEWVITELTGLDE
                     TVSLEGNFFQFDAEGGLFSAFLGCNNISGAYVFEDPSITFSEIARTEMECENMNSEEL
                     FCFLIEQVTSASVTEEGFLQLKSVEEEVLITLKKVEEEVEIVVEEPTEEQVEEPVEEQ
                     TEE"
     gene            5907..>6284
                     /locus_tag="PLI69_03010"
     CDS             5907..>6284
                     /locus_tag="PLI69_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOG41491.1"
                     /translation="MKSKLHLIPTPLGGENPRDVLPAPLFEVIGSLKLFFVEELRTAR
                     RFLSKAGLKGEIDKLTLYELNEHTSREQIECYVKYLTEQEGALISEAGLPAVADPGAL
                     LVEFAHQHDIEVIPYVGPSSLMMA"
BASE COUNT         1695 a         1297 c         1540 g         1752 t
ORIGIN      
        1 gaattactct gcgattgctt accgtttttc aggcaccaac aatgtcaaga gtttcggtat
       61 gagaaaagac tctgccaaag agtcattttc ggcaaatcta cgttggtttg gtttccagca
      121 gcagtttttc tccgccatac tcgttgctga aaataacttt gtgggcggca atctggaaaa
      181 caagttctat acggaggatg ttgcgattgc tacgggggac cttatgcgct cttcagcaga
      241 aatggtactt gacctggata ctcaaagcaa agatttttcc ctgcctttca agttctattt
      301 cggccccaat cactatccga cacttaagag ttacgatgag ggttttgaga agataattcc
      361 acttgggggc aaacttatag ggcctatcaa ccgctatgtc ataattccgg tattcaactg
      421 gctaagtaaa tatatctcaa gctacggttt gattattctt attctgacca ttttgatcaa
      481 gatcgtgatt tctcctctga ccttgaagtc ctataaatct tcagcaaaga tgaaggttct
      541 gcagcctgaa atcaagcaga tcaacgagag atatcctaag caagaagatg cgatgaaaaa
      601 gcaacaggcc acgatggacc tctataaaaa ggccggtgtg agcatgttcg gaggttgtct
      661 tccgatgttg ttgcagttcc cgattctatt tgcgatgttc cgtttcttcc ctgcttcttt
      721 cgagttgaga caacagagct tcttgtgggc tgacgacctc agcgcttatg actccatcct
      781 cgatttcgga ttcaatattc cactctacgg cgaccacatc tcccttttcg cgctcctaat
      841 gggtgtgtcg atgttcttct actccaggct gactatgagt cagatggcgg ataaccagca
      901 gatgccgggc atgaagttta tgcagacatg gtttatgcct atcttcatgg tgctgatctg
      961 taataacttc tccgcaggat tgagttatta ctatatgctc tcaaacctca ttactataat
     1021 ccagactctg attatcagaa aatggtttgt ggacgagaaa aaacttcgtg cccagattga
     1081 gctaagagcc agcagcaaag agccgcagaa gaagtccaag ttcagacagc gtctcgacga
     1141 ggcctacaga atacagcagg agcagatgag acaacagcaa aagcgcaaac gatgactata
     1201 cgagaaagaa tagagagtat tttgagcaaa ttgccaccta ccgtaaagtt ggtggcagtt
     1261 tcaaaactac actctgttcc ggagattcta gaggcatacg ttgcaggtca gcgtgcattc
     1321 ggggaaagcc gtccccagga aatggctgcc aaggtaccta tgcttcctaa ggatattgaa
     1381 tggcatttta tcggtcctct gcagagcaat aagataaaaa tgatcctgcc tcatgtttct
     1441 cttatccatt cggtagattc ggagcgtctt ctggcgagaa tagatcgcca ggctgctcgt
     1501 tccggcatct tggtggattg tcttatacag ctacatatag ccattgagga taccaaacaa
     1561 ggttttcccg tggatgaggc tctggaacag gttcctctct tccgtgacag gtataaaaat
     1621 gttcgtatcc gtggccttat gggaatggct tcgtttgtgg atgatatgga acaggtaagg
     1681 ggagagtttc gcactctgaa gtctgctttt gataagctta aacccgaatt ttcactcttt
     1741 gatactcttt ctatgggaat gtcgcaggat tggcacatag cggttgagga gggtgccaca
     1801 atagtacgta taggaagcac tatttttggt tcgcgctgat attgatattc taaataaata
     1861 actgtaactt tgtagattac gtgacggctt aatgtgattt gcaatgcata atgacttgtt
     1921 aggaaaagat ttgaggtatg gtctcatcgg tagcggcagt tgggcaactg cgcttgtcaa
     1981 ggtattgtct gatacgggaa atagagttaa ctggtatata cgcaattccg ataatatcga
     2041 ctttataaag gaatttggcc gaaaccgttt ctatctccgt tctgcggcat tgaagacagg
     2101 tatgctggag atgagcgacg atatcaatga ggttgtttac aattccgatg ctgtgatttt
     2161 ttgtatcccc tctgcatatt ttcttgccca gatcaagccg cttgacaatg accttttaaa
     2221 ggataaactc ctcatctcag ccaccaaagg attcgtttcc aacgatcatt tgacggttgc
     2281 ggagtatttt cacagagtat tgggcatacc gttcagcaga attgtagtat tgagtggccc
     2341 tacgcatgct gaagaggtag ctatggaaaa actctcctat ctcacttttg caagtaagca
     2401 tatcgaagtt tccagtaagg tgagtgaggc tttctcatcg cattatgtaa aggttgtctg
     2461 cagcacggat atatatggcg tagagtattc tgcagcaatg aagaatgtct atgccgttgc
     2521 tgtaggtctt tgccattccc tgggctatgg agataacttt atatctgtgc ttgtgacagg
     2581 agcctataat gaaatggtac gtttcctcaa ctcgactcat cccgattcca gccgcattcc
     2641 atcacgttca gcctaccttg gagatctcct ggtgacctgc tattcgcaat tcagccgcaa
     2701 ccgcaccttc ggaggcatga ttggcaaagg ctattctgtg gtttcggccc atactgagat
     2761 gaatatggtg gcggagggtt attatgccac aagatgcatg tatgagattg caaacaggct
     2821 caatgtcgag cttcctattg taaatgcgat gtatcagatt ctttacgaag agaaaagccc
     2881 ccattatgtg gtgaatcatc tggcctcgat tctcaagtaa acaacaatat aaaagtgacg
     2941 atgcaatgat taatctagat ttgaaaggat gttcaagctt tgtgaatgaa aagcagtttg
     3001 aggattatac tgccagagca cttgaggctt tatctatact tgattccggt accggtgcgg
     3061 gaagtaattt tctaggatgg aaccgccttc cttcggaaat cacacaggaa atgcttgagg
     3121 actacctttc tgtggttgac aaatggattg aaatggagat tgatctggta gttgtcattg
     3181 gcattggcgg ctcatatctt ggtgcaaagg ctgctattga ggcgctctcg cattcattcc
     3241 atcagatggg tccatcccga ggtcccaaag ttgtttttgc gggacacaat ctttctgaag
     3301 agtatattgc cgaactaatg gatatggtgt cggtaaagaa tgttgcctgt gttgtgattt
     3361 ccaagtcagg taccactaca gagccggccg tggtgttccg tttgatcaaa aaatacattg
     3421 aagagacata tggcattaag gaggcccgca aaaggattat tgcggttacc gacaaggaac
     3481 gcggagccct gagattgctc tccaatcagg agggttacaa gacttttgtc atcgaagaaa
     3541 atataggggg acgcttttca gtattgacag cagtgggatt gctgcccata gcactggccg
     3601 gatttgatat tacagaaatg gtcaggggag ccgtggatat gcagcggatc tgtagcgaac
     3661 cgacaaagga taatcccgcc atccgctatg cagctatgcg caatattctc tatgataatg
     3721 gcaaaaagat agaactcctt gtaggattta atccaaaact tcactatctt agtgagtggt
     3781 ggaaacagct ctttggtgag agtgaaggca aagatggcaa aggcatattt ccggcatcgg
     3841 tgaactttac cacggatctc cattccctcg gacaatatat ccaggatgga gaacgcattc
     3901 tctttgagac attcattgat gtgcgtacaa tccggaggaa agtggaaatc ggttacgata
     3961 agcaggacct ggacggactc aattatctcg caggcaagaa tgtggaattc tgcaacaaga
     4021 tggctcagca gggaacaatg tttgcccata atgacggtgg agtgcctacc atggaaatat
     4081 ctatagactt gcttgacgaa tataacctcg gccagctctt ttactttttt gaaaaagctt
     4141 gtggcataag tgcgtatata ctgggtgtta atccttttga ccagccggga gttgaggatt
     4201 acaagaaaaa catgtttgct cttctgggaa aaccggggta tgaacgacag agtaaagagc
     4261 tacgtaagag actttgacac attctatata attatgaaaa cgaaatatat tttgatgctg
     4321 tttttggcgg catcgcttct ggtttcctgt aatacacagg agcaaaaaga gcctgaagag
     4381 tgggccattg tattgcacgc aggagcaggt ggcactcagg caatgtctgt tgaggatgag
     4441 gcatcttatt gcatttttct aaatgaagcc ctggagcagg gaaaaaccat tctttcatcg
     4501 ggtggtagcg cgcttgacgc tgtggaggca actgtgatct atcttgaaga ttgccctctc
     4561 tttaatgcag gaaaaggggc agtaatgact tcggatggac gtcatgaact tgacgcctca
     4621 ataatggacg gccgcgattt ctcggcaggt gccgtagccg gtgtgaccga cataaagaac
     4681 cctatcaaag ctgcacgttt ggtaatggaa aagacccctc atgtcttgct tatcggagag
     4741 ggagcttcct tttttgcacg cgaacaggga ctcgaaatag ttcccaattc ctatttttct
     4801 actgagagca gcaaaataga gctcgaaaga gctctcgcag agaaaaagag tggctctcct
     4861 ataggtacag tgggttgtgt ggctcttgat agtgatggaa atattgctgc tgctacctcc
     4921 acgggaggca tgaccggaaa gaaatggggc agggtaggag atgtgcccat cattggtgcc
     4981 tctaccattg ctgataataa cttcgtaggt gtctccagta caggtcacgg tgaactctgg
     5041 atccgcaggt ctgtagctca tgatatctac gctctgatgg actacaaagg tttttctctt
     5101 gaagaggcag ttcacgaggt tatctggaac aaaatagaca agatggaggg ctccggcggt
     5161 ggtgtgattt gcattgaccg tttcggcaat atcgtgatgg atttcaacac ccatataatg
     5221 tatcgtgctt gggcaaaagc ttccggagaa tccggtgcag cagttaataa ataacactaa
     5281 ccaaccaaaa tataactaaa atgaaaaaat taatcaatta caccattgta ctcctggccg
     5341 caatttcatt ggcaggatgt ggtacgccca aagaggctcc catagattta aacggggagt
     5401 gggtaattac tgagttaacc ggccttgacg agaccgtttc tctcgaggga aatttcttcc
     5461 agtttgatgc ggaaggagga ctcttttctg cctttctcgg atgtaacaac atttcaggtg
     5521 cgtatgtgtt tgaagatccc tcaatcactt tttccgaaat cgctcgcaca gagatggagt
     5581 gtgaaaacat gaactcagaa gagctattct gcttccttat cgagcaggtg actagcgctt
     5641 cagtaacaga agaaggcttt ttacagttga agtctgttga agaggaagtt ctgatcaccc
     5701 tcaagaaagt agaggaggaa gtagagattg tagtagaaga gccgacagag gaacaagttg
     5761 aggagccggt agaggagcag accgaggagt aatccgcata tctctgctat atctctttaa
     5821 ggaattgaag gtctatgaca tccgggtcgt agacctttat tccttttatt acttcatatt
     5881 cctctctttt tgctaccttt gtttcgatga aatccaaact ccatctgata cccactcctc
     5941 ttggcggaga aaatccgcgg gatgtgcttc cggctccgct ttttgaagtg atcggctcac
     6001 tgaagctttt ctttgtggag gaacttcgta ctgcccgcag atttctttca aaggcgggac
     6061 tgaagggaga aattgataag cttactctct atgagctcaa cgagcatact agtcgtgaac
     6121 aaattgagtg ttatgtaaaa taccttacag agcaggaggg ggctctcatc tcggaagcgg
     6181 gactcccagc agttgctgat ccgggtgcat tgctggtaga gtttgctcat cagcacgata
     6241 tcgaagtgat tccctatgtg gggccctctt cactcatgat ggcc
//
LOCUS       DAOSIE010000062         6227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_11382_length_6227_cov_4.261342, whole genome shotgun sequence.
ACCESSION   DAOSIE010000062 DAOSIE010000000
VERSION     DAOSIE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11382_length_6227_cov_4.261342"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="PLI69_03015"
     CDS             <1..122
                     /locus_tag="PLI69_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007368362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOG41492.1"
                     /translation="VPEVLLSGNPKLIKAWQEEQALARTRRLRPHLLKENQKE"
     gene            complement(100..1560)
                     /locus_tag="PLI69_03020"
     CDS             complement(100..1560)
                     /locus_tag="PLI69_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG41493.1"
                     /translation="MKTARTARIVIAALFFIVITLSFISFSAGKLPFSPAMEKFQFGP
                     ALMALFVGSALPLILLLILTLVLGRVYCSFLCPLGIWQDIVIWIADRFKSKKKRRHNY
                     RPAHNTLRYIILGVVTAALIAGISYPFAFLDPYSIFGRIVTSFNPYTSFAIWSFVVAM
                     VFFLTVTILSAFRGRLYCNTICPVGSFLGLVSSHSMFRLTIADKQCVECSLCGKKCKA
                     EALDCVTKTIDYSRCVMCFDCTISCKKKAITLKYAWGSGKRDKCTSETKKDPESAGRR
                     NALIALGTLGLVAASRKWGSKFYSSPVSDATDNPVVQFLPPGAMNLEHLKEHCTACHA
                     CIAACPSKIIRPSITQHGIDGFLMPMLSFDENYCHFDCNKCSNVCPNGALKPLTLSEK
                     RSMRIGMVKFVAKNCLVFRDDQVCGKCGEVCPTGAIKLREHKRKPGLFFPVVNSDLCI
                     GCGGCTSVCPAEPLAMEVHALEYQLSITRVTLSDSL"
     gene            1699..1920
                     /locus_tag="PLI69_03025"
     CDS             1699..1920
                     /locus_tag="PLI69_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41494.1"
                     /translation="MKKLLLLIAVGALFLCAGCSKKCHCVAKLNGEVVYERSDIVLGE
                     GERCSDYNTMVSVPLVATKVESKCLPQLF"
     gene            1995..2879
                     /locus_tag="PLI69_03030"
     CDS             1995..2879
                     /locus_tag="PLI69_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOG41495.1"
                     /translation="MKQELKYLLRVLLGLFFIISAVAKLLVIDPFEIYIFGFRMFGLG
                     LSFFIARAVIALEFALGILLILNMHSQLIYYSSLVVLGIFTLFLLGLTIIGNRDSCYC
                     LGELVDMQPWQSLIKNILLLVMLRLSAGMKSFRIPWKPLWYTLAIAGSTAAVFIISPP
                     DNWRYNQYGRSTVVSEKALEHAFSEGLLPSELLEGEKVVCFMSTTCEYCLMAAQKITI
                     LRSRGDFSNGELTVVFVQGNVELDPSVFLENAELDYEQYLFLDVNPFIRITEGTMPLT
                     LVLNDGKIVAKYSYRDIR"
     gene            complement(2861..3733)
                     /gene="folD"
                     /locus_tag="PLI69_03035"
     CDS             complement(2861..3733)
                     /gene="folD"
                     /locus_tag="PLI69_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOG41496.1"
                     /translation="MILLDGKATSEAIKKEIASQVDKIVASGGRRPHLAAIIVGEDGA
                     SKTYIASKERACAQVGFDSTVHRLSEQTTEEELLNLIARLNADKSVDGFIVQMPLPRS
                     IDEQKVIAAVDPLKDVDGFHPVNVGRLSLGLPTFVSATPYGIMALLEHYKIETRGKHC
                     VVLGRSNIVGRPIANLLSQKREPGDCTVTMCHSRTKNLAEYTHRADIIIAALGVPEFL
                     KGDMVKEGAVVVDVGITRIPADNEKGYRIVGDVAFDEVAPKCSYITPVPGGVGPMTII
                     SLLRNTMQAYLMSR"
     gene            complement(3746..5071)
                     /gene="ffh"
                     /locus_tag="PLI69_03040"
     CDS             complement(3746..5071)
                     /gene="ffh"
                     /locus_tag="PLI69_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOG41497.1"
                     /translation="MFENLIERLESSFKLIKGQGRITEVNISETLKDIRKALLDADVS
                     YKIAKTFCDEVKQKALGQDVLKAVRPEQMMIKIVHDELALLMGSEASDINVKGNPGIV
                     LVAGLQGSGKTTFSAKLALNCKSKRGMRTLLVAGDVYRPAAIDQLKVLGEQIGVEVYT
                     EDGVKEPVSIAKNAVKKARNEGYSLVIIDTAGRLAIDKQMMDEISSIKSAVNPTETLF
                     VVDAMTGQDAVETARAFNEVLDFDGVVLTKMDGDTRGGAALSIKAVVQKPIKFISAGE
                     KMEALDVFHPKRIADRILGMGDVVSLVEKAQEQYDEEEARRLHKKMARNQFNLDDFYQ
                     QIQQIKKMGNIKDLASMLPGVGKAIKDVDIDNSSFKGVEAIILSMTPYERENPSVLNG
                     SRRKRIAEGSGTSLVEVNRLLKQFEGTRKMMKNFSSNAMSQRVMRKKRR"
     gene            complement(5198..6046)
                     /locus_tag="PLI69_03045"
     CDS             complement(5198..6046)
                     /locus_tag="PLI69_03045"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOG41498.1"
                     /translation="MTLLQEEETVTSTCAYFGMTRQAYYKSRKRVENKALNDYMAVEL
                     VQEKRRDLPRLGGRKLYYLLKDDLSKIGRIGRDKFFDILGRHDLLVKPKKSFTRTTNS
                     FHYFYKWKNLIKDLSITRVNQVWVSDITYIRTLSGFAYLFLTTDLYSRKIVGWTLSSS
                     LGIESGIRALKMALSSRGSTTESLIHHSDRGIQYCCKDYVKTLQEAGIDISMTEDNHC
                     YENAVAERVNGILKNEFYLDNTFNSFEQALSAVGSGIKNYNEKRPHWSLDLQTPSQVY
                     SQTKVA"
     gene            complement(6016..>6227)
                     /locus_tag="PLI69_03050"
     CDS             complement(6016..>6227)
                     /locus_tag="PLI69_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41499.1"
                     /translation="IVRVETRDEIDELKRLRAENEALKKAYAELALHHKCSEKVIEVA
                     DELFDMDLKKKYEQELSLYFKKKKQ"
BASE COUNT         1624 a         1517 c         1303 g         1783 t
ORIGIN      
        1 aagtcccgga ggtgctcctg agcgggaatc cgaaactcat taaagcatgg caggaagagc
       61 aggcactagc acgtacccgc cgtctgcgtc cccaccttct taaagagaat cagaaagagt
      121 aactctagtg attgacagct gatattccaa ggcatgaact tccattgcca aaggctcagc
      181 gggacatacg ctggtacagc ctccgcaacc tatgcataag tcgctattga ccaccgggaa
      241 aaagagtccg ggcttccttt tgtgctctcg tagcttgatg gctcctgtag gacaaacctc
      301 accgcattta ccacagacct gatcatcgcg aaataccaga caatttttgg cgacaaactt
      361 gaccatacct attctcatcg aacgcttctc actgagtgtt agaggcttca gggctccgtt
      421 cggacagaca ttggaacact tgttacagtc gaaatgacaa taattctcat caaaggaaag
      481 catcggcatt agaaacccat cgatgccatg ctgggttatt gagggtctta tgatcttcga
      541 agggcaagcg gctatacaag cgtgacaggc tgtgcaatgc tctttaaggt gttccagatt
      601 catagctccg ggagggagaa attgcaccac cggattatcc gtcgcatctg aaacaggaga
      661 agagtaaaac ttcgatcccc attttctgga agctgccacc aatccgagag tgccaagcgc
      721 aatcagggca tttctacggc ctgcgctttc cgggtccttc ttagtttcag aagtgcattt
      781 atcgcgttta ccgctccccc aggcatattt tagcgttatt gccttcttct tgcaagagat
      841 tgtacaatcg aaacacatca cacatcgtga ataatcgata gttttggtaa cgcaatccaa
      901 cgcctctgcc ttacatttct taccgcaaag ggagcattct acacactgtt tatcagctat
      961 agtcaggcga aacatcgaat ggctgctaac cagcccgagg aaggagccca caggacagat
     1021 ggtattgcag taaagacggc ctctaaatgc acttaaaatg gtaaccgtga ggaagaatac
     1081 catggccact acaaacgacc atatggcaaa cgaagtataa gggttgaatg aggtaactat
     1141 cctaccaaat atcgaataag gatccaggaa agcaaatgga taggaaatgc ctgctatcaa
     1201 cgcggctgta accactccca gaattatata tctcagcgta ttgtgagccg ggcgatagtt
     1261 atgtcggcgc ttctttttgc tcttaaagcg atcggcgatc cagataacaa tgtcctgcca
     1321 gatccccaaa ggacaaagaa aagagcaata gacccttccg agtaccaacg taagtatcag
     1381 cagcaatatc aagggaagtg cacttcccac gaatagtgcc ataagagccg gaccgaactg
     1441 gaatttctcc atcgctggcg aaaagggcag ctttccggct gaaaaggaga tgaaagaaag
     1501 tgttatgaca ataaagaaaa gggctgcaat gactattctt gccgttcttg ctgttttcat
     1561 ctttgaattc taaattttcg tcaaaagtac ttttttattt tcatatgtta aaaataaagg
     1621 ctacttttgc ggcggtccgg aagtctggat caatatttgt agggaacaaa gaaatatact
     1681 taaataagaa ttattacaat gaagaagtta ctacttttaa ttgcagtagg agcccttttc
     1741 ctctgcgccg gttgttcgaa gaaatgccac tgcgtagcca aactcaatgg tgaagtagtg
     1801 tatgaaaggt ctgacatcgt tcttggtgaa ggtgagagat gctctgatta caatactatg
     1861 gttagcgtac ctctcgttgc tacaaaggtc gaatcgaaat gccttccgca gctcttctaa
     1921 caaagtgaaa aagattaatg agggggtgat ctaggtcacc ctctttttgt aataattcat
     1981 aactaaagga cactatgaaa caagaactca aatatctact tagggtattg ctaggcttat
     2041 ttttcataat ttcagcggta gctaaactcc tagtgattga cccattcgaa atctatatat
     2101 tcggattccg aatgttcggg ttaggactct ccttcttcat cgcaagggca gttattgcat
     2161 tggaatttgc cctcggcata ctcctcattc taaacatgca ttcgcagctg atatactact
     2221 catcattagt ggttctgggt atcttcactc tttttctctt gggactcacc attatcggca
     2281 accgagacag ttgctactgt ttaggcgaac tggtagatat gcaaccctgg cagtccctga
     2341 taaaaaatat attactactt gtaatgctac gtctgagcgc aggcatgaaa tcctttagaa
     2401 taccctggaa gccgctatgg tacacgctcg ctatagcagg ttctacagca gctgtattta
     2461 ttatctctcc tcccgacaac tggaggtaca accaatacgg tcggagtact gttgtaagtg
     2521 aaaaagccct cgaacatgcc ttttctgagg gattactgcc gtcggagctg cttgaaggtg
     2581 aaaaggtagt ttgttttatg agcaccacct gtgaatactg tctgatggct gcacaaaaaa
     2641 tcacaatcct tcgcagcagg ggagatttca gcaatggaga gctgacggtt gtatttgtac
     2701 agggtaatgt tgaacttgat ccatctgtat ttcttgaaaa tgcagaactt gactatgaac
     2761 aatacctctt cctggatgtc aatcccttta tcaggattac tgaaggcact atgccgttga
     2821 cgcttgtgct taacgatggc aaaatagtgg ctaaatatag ctaccgagac atcagataag
     2881 cctgcatagt attgcgcagg agagatatta tggtcatagg accaacgccg cccggcaccg
     2941 gagtgatgta agagcatttc ggtgcaacct cgtcaaaggc tacgtctcct acaatcctat
     3001 accctttctc attatccgcc ggaatgcggg taatgcccac atccaccacc accgctccct
     3061 cttttaccat atctcctttc aggaactcgg ggactccaag tgccgcaata atgatatctg
     3121 cccggtgtgt gtactcggcc agattttttg tgcgactgtg gcacatggtg accgtgcagt
     3181 ctcccggttc tcttttttgc gaaagcagat tggctatcgg acggcctaca atattgctgc
     3241 gacccaatac cacacaatgc tttccgcgag tctcaatttt gtagtgctcc agcagggcca
     3301 ttataccata aggagtggct gaaacgaagg tcggaagtcc aagcgagagt ctgcccacat
     3361 ttacaggatg aaaaccgtca acatccttga ggggatctac tgcggctatg actttttgct
     3421 cgtcaataga gcgcgggaga ggcatttgga cgatgaaacc atctaccgac ttatctgcat
     3481 tgagtcgggc gataagattg agaagttcct cttctgtcgt ctgctccgat aaacgatgca
     3541 ccgtagaatc aaatcccact tgcgcacaag cacgctcttt tgaagcgatg taggtcttgc
     3601 tggcaccatc ctctccaacg ataattgccg caagatgagg tcttctacct cccgaagcca
     3661 caattttatc tacctgtgaa gctatttctt tttttatggc ctccgatgtg gcctttccgt
     3721 cgagtaatat cattgccttg aagtcttatc tgcgtttttt tctcatcact ctctgtgaca
     3781 ttgcattgga tgaaaagttc ttcatcatct tacgtgtacc ctcaaattgc ttaagcaaac
     3841 gattgacctc caccaatgag gtaccgctgc cctctgcaat cctcttgcga cgactgccgt
     3901 ttagcacgga gggattctcc ctttcgtaag gagtcatgga aaggatgatg gcctccactc
     3961 ccttaaagga actgttgtcg atatctacat ccttgatggc tttgccgacg ccgggaagca
     4021 tagatgccag atccttgatg tttcccatct tctttatctg ctgaatctgc tggtagaaat
     4081 catcaaggtt gaactgattt ctggccatct tcttgtggag cctgcgggcc tcctcttcat
     4141 catactgctc ctgagccttc tccacaaggg aaaccacgtc acccataccc aggatacggt
     4201 cggctatgcg cttgggatgg aatacgtcca gagcctccat cttctcacct gcacttataa
     4261 acttgatggg cttctgcact actgctttga tggaaagggc tgcacctccg cgggtatcgc
     4321 catccatctt ggtaagcact actccgtcaa aatcgagcac ttcattgaaa gccctcgccg
     4381 tctccactgc atcctgaccg gtcatcgcat caactacgaa aagagtctcg gtgggattca
     4441 ccgcactctt tatggaggaa atctcatcca tcatctgctt atcgattgca aggcggccgg
     4501 cagtgtctat gattactagc gaataaccct cattgcgtgc cttcttcact gcattctttg
     4561 caatcgaaac gggctccttg actccatcct ccgtatatac ttcgactccg atttgctccc
     4621 caagcacctt caactgatcg attgcggcag gacgatagac gtcacctgcc acgagaagcg
     4681 tacgcatgcc cctctttgac ttgcagttga gcgccaattt agctgagaac gtggttttac
     4741 ccgatccctg aagacctgcc accagcacaa tgcccggatt gcccttgaca ttgatatcgg
     4801 aagcctcgct gcccatgagg agagcaagtt cgtcgtggac gattttgatc atcatctgct
     4861 caggccgaac tgcctttaag acatcctgac caagtgcttt ctgctttact tcatcacaga
     4921 atgtctttgc tatcttataa cttacgtcgg catcaagaag tgccttgcgg atatccttga
     4981 gcgtctcgga gatatttacc tccgttattc ttccttgtcc cttgatgagt ttgaacgaac
     5041 tctcaagtct ttctatcaga ttctcaaaca tattctctat actcttactc tatactctta
     5101 ctctatactt tcaatactca agactttctc ccctgtctcg tcctaaaaaa agttgacaca
     5161 ttttgttaaa cattattgta ttcacttttt cttttattta tgccactttt gtctgactgt
     5221 aaacttgaga cggagtttgc agatctaatg accagtgcgg gcgtttctca ttgtaatttt
     5281 ttatccctga gcctacggct gaaagggctt gctcaaagct gttaaacgta ttatctaaat
     5341 aaaattcatt cttcaatatg ccgttcacac gctctgcaac ggcattttcg taacaatggt
     5401 tatcctcggt catgctaata tcaatccctg cttcctgaag cgttttcaca tagtctttgc
     5461 aacaatactg tatgcctctg tcagagtgat gtatcagcga ttcagtcgtg ctgccacgtg
     5521 aagaaagagc catttttagg gcccggattc cactctctat tcccaaactg ctacttagcg
     5581 tccagcctac tattttcctg gaatacaagt cagtagttaa aaacagatag gcaaagccac
     5641 taagcgttct gatgtaagtt atatcgctta cccacacttg gtttacccgt gttatggata
     5701 aatcctttat caagtttttc cacttgtaaa agtagtggaa agagttggtt gtcctggtga
     5761 aacttttctt gggtttcaca agcaaatcgt gtcgaccgag tatatcaaag aacttatctc
     5821 ttccgatcct gcctatttta gacaaatcat cctttagcaa atagtagagt tttcttccac
     5881 ccaaacgggg taaatctcgt ctcttctctt gaactaactc aactgccata tagtcattaa
     5941 gtgccttatt ttcaacacgt ttcctgctct tataataggc ttgacgagtc attccaaagt
     6001 aggcacaagt tgacgttact gtttcttctt cttgaagtaa agtgataact cttgttcgta
     6061 cttttttttt aagtccatgt caaaaagctc gtcagctact tctatcacct tttctgagca
     6121 tttatggtgc agagcaagct ctgcataggc ttttttcaag gcttcatttt ccgctcgaag
     6181 gcgtttcaat tcgtctattt catctcttgt ttccacgcga actattt
//
LOCUS       DAOSIE010000063         6222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_11399_length_6222_cov_4.628831, whole genome shotgun sequence.
ACCESSION   DAOSIE010000063 DAOSIE010000000
VERSION     DAOSIE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11399_length_6222_cov_4.628831"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /gene="pyrH"
                     /locus_tag="PLI69_03055"
     CDS             <1..588
                     /gene="pyrH"
                     /locus_tag="PLI69_03055"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOG41500.1"
                     /translation="IVIGGGNIFRGLAGTERGFDRVKGDQMGMLATVINSIGLSLSLR
                     NAGLKAEVFTATPMRPMARYYMRDEVLEYMEQGGVAVVAGGTGNPFFTTDSAAALRAC
                     ELGVDALLKGTKVDGVYDKDPKKHSDAVKYRSITFDQAIDDNLRVMDQTAFTLCKEND
                     MPIIVFDINHEGSLKRLVAGEEVGTIVSNHVKTII"
     gene            607..1170
                     /gene="frr"
                     /locus_tag="PLI69_03060"
     CDS             607..1170
                     /gene="frr"
                     /locus_tag="PLI69_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOG41501.1"
                     /translation="MIEKAKEIIASTSQKMAEAVEFLDEDLKTYRAGKANPAVFNAVM
                     VNYYGSMTPVPQVASINTPDAKTIIIQPWDKKMIPAIEKAIMDANIGFTPQNNGEQIR
                     INVPALTEERRRDLVKKAKTAGENAKISIRNARREGIETLRKLQKEGMPEDVVKVSEG
                     DIQKETDTFTKNVDELLAEKEKEIMTV"
     gene            1170..2207
                     /locus_tag="PLI69_03065"
     CDS             1170..2207
                     /locus_tag="PLI69_03065"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOG41502.1"
                     /translation="MIQIICRPCSAESEEQVLATASALVDILAGSKDYRHIAFRAGLW
                     KPRTRPGTFEGVGEAALPWLQRVQRETGLKVIVEVATAAHLEAVLKAGIDMVWVGART
                     TANPFAVQELADALKGVDIPVFVKNPLNPDLNLWLGAVERIEMAGIRDISVIHRGFSF
                     YEKGRYRNEPKWQVPIDLMRRRPDLRILCDPSHITGKREYLPEVSQMAMDLGFDGLFI
                     ESHISPDSALSDPEQQIEPHSLGRMLEDLRIRSSIPEDEELSRRLQELRARIDSLDED
                     ILEILELRMGVVDQIGAQKRRGNITILQQQRWEEILEKTRSGALSRGLDPGFVEELFK
                     LIHIASIRRQV"
     gene            2407..3867
                     /locus_tag="PLI69_03070"
     CDS             2407..3867
                     /locus_tag="PLI69_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41503.1"
                     /translation="MGKLLLMAAVLLVATFSANAKTDKVELCDGKIVVTVKDIAPNAD
                     LTGLVTNYTITVAPDVIEQCTALILTPAVRDTDTKSKRVVEILVISAPGERYRSMWVE
                     EQLYKVCDPNRVKFYTAKKGESLTIETNTLVPYESWMDDAKFTVTTQKGTYNPDCIEN
                     VCGTEEVCDIPYLSSPLTVDPLWTAVTPVLVDIDPIRALKTKLYYPVNVTRSVESYLE
                     NQDALALLGTLNQPNFTVASIKIGGWASPEATVAYNQKLSEKRAATLKGIIAGKYNFP
                     ESVYSVSGNGEYWDDVINFIATTDDPTVKSSKDELEAAIANNANLDAREAAMRRVDRG
                     RPYKVIFDAVYPRSRFADCVVSYQLRGFNREHAMVVFRTDPYQLTENDYIQLLAVNPD
                     PVVLEKAVEIYDNESLYAIAAKAEADAGNFDQAIKYYQKAGDSKEVMNNIACCYLLKG
                     DAKNAEKYLEKAKGLDVYEGNQNELRKVVLNNKYFK"
     gene            3999..4084
                     /locus_tag="PLI69_03075"
     tRNA            3999..4084
                     /locus_tag="PLI69_03075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4033..4035,aa:Leu,seq:taa)
     gene            complement(4141..5526)
                     /gene="radA"
                     /locus_tag="PLI69_03080"
     CDS             complement(4141..5526)
                     /gene="radA"
                     /locus_tag="PLI69_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOG41504.1"
                     /translation="MAKTKSAWFCSSCGNESPKWMGRCPACGEWNTMIEEPKAPSGTG
                     GGASGRNRSFLQTQEARPQLLTGITFDEEQRFFIGNGELDRILGGGMVQGSMILIGGE
                     PGIGKSTLSLQIPLHAEGLKTLYVSGEESPVQIKLRAQRLQGDDSQCYVLGETLLENI
                     LLQAREVNPSLLIIDSIQTIYSQSIESSAGSVSQVRECASVLLRYAKESNVPVILIGH
                     ITKDGAIAGPKILEHIVDVVLLFEGDTKGAYRILRSIKNRFGSTDELAVFEMTGSGLR
                     EVSNPSEILMPMHKDDLSGVAVSAMMDGTRPFLIEIQSLVSTAAYGTPQRSTTGFDVR
                     RLNMLLAVLEKRAGFRLAAKDVFLNIAGGLRVSDPACDLAIVAAILSSNFDLHISPKI
                     CFAAEVGLSGEIRPVSQVERRIAEAEKLGFEVIFISSYNKESSFAKRPKGGITVIPIS
                     DIPTLCKKLFK"
     gene            5635..>6222
                     /locus_tag="PLI69_03085"
     CDS             5635..>6222
                     /locus_tag="PLI69_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HOG41505.1"
                     /translation="MINFNIEYKTHFGQQLYVAGSIPELGEWDYFRALPMAYSDGGNW
                     KANIKTPSGTFSYKYILKSPSGILVEVGEPRTISAGGRSGNISLYDMWQGSSDHSAFL
                     SAPFENVFYSRKSLQTPVETDFAKEVVISVTAPLVHSDDSISICGECDALGNWNPLKA
                     LPMRPLPGCKWEVALDASLLPKLVRFKFIKIIGDTA"
BASE COUNT         1719 a         1422 c         1502 g         1579 t
ORIGIN      
        1 atagttattg gaggtggcaa cattttccgc ggtctcgccg gcacggaacg cggattcgac
       61 cgtgtcaaag gcgatcagat gggtatgctc gctactgtaa tcaacagtat cggactctcc
      121 ctttcgctga gaaatgccgg actcaaagct gaggttttta ccgcgactcc catgcgcccg
      181 atggccagat attatatgcg tgacgaagtg ctggaatata tggaacaagg cggggttgcc
      241 gtggtggccg gcggtacggg taatcctttc tttactactg actctgctgc ggctctaagg
      301 gcttgtgaac ttggtgtaga tgctcttctg aagggtacaa aggtggacgg agtttatgat
      361 aaggacccca aaaaacactc cgatgcggtt aaataccgct ccataacatt tgatcaagcc
      421 atcgatgaca atctccgagt gatggatcag accgcattta ctctttgcaa agagaatgac
      481 atgcctatca ttgtcttcga cataaaccac gaaggtagcc taaaaaggct cgttgccgga
      541 gaagaggtgg gcaccatagt atccaaccat gttaagacaa ttatttaata ttcaaaaccg
      601 actacgatga tagagaaagc aaaagaaata attgcttcga ccagtcaaaa gatggctgag
      661 gccgtagagt ttctggacga agatctcaaa acttaccggg caggaaaggc aaatccggca
      721 gtgttcaatg cagttatggt taattattac ggatcgatga cccccgtccc tcaggtggcc
      781 agcatcaata ccccggatgc aaagactatt attattcagc cctgggacaa aaagatgatt
      841 cctgccatag agaaggccat catggatgct aatatcggat ttactccaca gaacaatggt
      901 gaacaaatcc gcatcaatgt gcctgctctt acggaggagc gtcgccgcga cctggtaaaa
      961 aaggcaaaaa ctgccggaga gaatgcaaag atcagtattc gtaacgctcg tcgtgaaggt
     1021 atagaaacac tcaggaagtt acaaaaagag ggaatgcctg aagatgtagt aaaagtttcc
     1081 gaaggcgaca tccagaaaga gactgacacc tttaccaaaa atgttgacga attgctcgcg
     1141 gagaaagaga aggagataat gacagtataa tgatacaaat catctgcaga ccctgcagtg
     1201 ccgagtccga agagcaggtg ctggctacgg catctgctct tgttgatatt ttggcaggca
     1261 gcaaagatta tcgccatatt gcctttcggg cagggctctg gaagccccgt acccgccccg
     1321 ggaccttcga aggggtgggt gaggcggctc ttccgtggtt gcagcgggtg caacgcgaga
     1381 ccggtctgaa agtcattgtg gaggtggcta cggctgcaca tctggaagcg gtactaaagg
     1441 cagggataga tatggtttgg gttggagccc gtactacggc caaccccttt gcagtacagg
     1501 agttggcaga tgccctgaag ggagtggata ttcccgtatt tgtcaaaaat cccctcaatc
     1561 ccgacctcaa cttgtggctg ggagctgtcg aaaggataga aatggcgggc atacgagaca
     1621 tttcagtcat tcacagaggc ttttcattct atgaaaaagg tcgttaccgt aatgaaccca
     1681 agtggcaggt tcccattgac ctcatgcgca gacgccctga tttgaggata ctctgcgatc
     1741 ccagtcatat tacaggcaag cgggaatatc ttcccgaagt atctcaaatg gcaatggacc
     1801 tgggttttga cggtcttttc atcgagagtc acatatctcc cgactccgca ctcagcgacc
     1861 cggaacaaca gattgagccg cactctctgg gccggatgct tgaagatctc aggatacgca
     1921 gcagcattcc tgaagacgaa gaactcagca ggagacttca ggagctcaga gcacgaattg
     1981 actcgcttga cgaggatatt ctggaaattc tggaactcag aatgggagtg gttgatcaga
     2041 taggtgcgca gaaacggcgt ggaaatataa ctattctaca gcagcagcgc tgggaggaaa
     2101 ttctcgagaa gacacgcagc ggtgcgttat cccgcggcct cgaccccgga tttgtagagg
     2161 aactattcaa actgatccat atagcctcca tcagacggca ggtgtagtgt tggggggtgc
     2221 gaattgcagg ttgcatgttt cagtttacgt ggtgcgggtt gtcataagaa aaatatttaa
     2281 aaaatattat tattgtttac aaattatttc ctatctttgg cattgccaat tgtatctaaa
     2341 aaagagtatt aacggtaaac tagaaaatta ataataagaa acttaaaaca aaacatgatg
     2401 aaaaaaatgg gtaaattgtt gctaatggcg gctgtgctgc ttgttgcaac attctctgct
     2461 aatgccaaga ctgacaaggt tgaactctgt gatggcaaga tcgtagtgac tgttaaagat
     2521 attgctccaa atgcagatct tacaggcctc gttacgaact acaccatcac ggtggctccc
     2581 gatgttatcg aacagtgtac tgcactgatt ttgacaccgg ctgttaggga tacagatacc
     2641 aaatccaaga gggttgtcga gattctagtt atcagcgctc ccggagaaag gtatcgtagt
     2701 atgtgggttg aagagcagct ctacaaggta tgcgacccca accgcgtaaa attctacact
     2761 gccaagaagg gcgaatccct caccattgag acaaacactc tggttcccta tgagagctgg
     2821 atggatgatg ccaagtttac ggtgactaca cagaaaggta cttacaatcc cgattgtatc
     2881 gagaatgttt gtggtaccga agaggtttgc gacattccct acctaagcag tccgcttaca
     2941 gtagatcctc tatggactgc ggtaactcct gttttggttg acattgatcc tatcagggca
     3001 ctcaagacca agctctacta tcctgtaaat gttaccagat ctgttgagtc ctatcttgag
     3061 aaccaggatg ctcttgcgct tttgggtaca ctcaatcaac ccaactttac tgtggcaagc
     3121 attaagattg gcggttgggc atctcctgag gctacggtag cttacaacca gaaactttct
     3181 gagaaacgtg ctgctaccct caagggcatc atcgcaggaa aatacaactt ccccgagagc
     3241 gtttactcag tatcgggtaa tggtgagtac tgggacgatg tgatcaactt catcgcaact
     3301 actgatgatc ctaccgtaaa atcttcaaag gatgaactcg aagctgccat agctaacaat
     3361 gccaatcttg acgccagaga ggctgctatg agaagagtag acaggggcag accctataag
     3421 gttatcttcg atgcagttta ccccaggtca cgttttgcag actgtgtggt ttcataccaa
     3481 ctcaggggct tcaacagaga gcatgcgatg gtagttttca gaactgatcc ttatcaactc
     3541 acggagaatg actacatcca gcttcttgca gttaatcccg accccgtagt actcgaaaag
     3601 gccgtagaga tttatgacaa cgaaagtctc tatgcaattg ctgcaaaggc tgaagccgat
     3661 gccggtaatt ttgatcaggc catcaaatac taccagaagg caggtgattc caaggaagtg
     3721 atgaataaca ttgcttgctg ctacctccta aagggtgatg ccaagaatgc tgagaagtat
     3781 cttgagaagg ccaagggtct ggatgtttat gaaggcaacc aaaacgaatt gagaaaagta
     3841 gttttgaaca acaagtactt caagtaacgg gttcaattct caatttcgag tgagttatgc
     3901 tgcccggcac attgtcgggc agcatttttt tgataccata ttgccaaact gcaattattt
     3961 cgtacatttg caactccaag gcgaaagccg cattacctgc tcaggtggtg gaataggtag
     4021 acacgcagga cttaaaatcc tgtgggcagt aatgcccgta cgggttcgat tcccgtcccg
     4081 agcacaaagt cccgtaagaa actccttacg ggactttgtg tttttattga tagtgaaaaa
     4141 ctatttgaat agcttcttgc aaagcgtggg gatgtcggaa attgggatga cggtgatgcc
     4201 tcctttggga cgctttgcaa aagaactttc cttgttgtat gaagagatga aaatcacttc
     4261 aaatccgagt ttctcggcct cggcaatgcg tctttccacc tgactgaccg gccgaatttc
     4321 accacttaac ccaacttccg ctgcaaaaca gatcttcgga ctgatatgta gatcaaaatt
     4381 ggaggagaga attgctgcaa ctatcgccaa atcacaagca gggtcactta ctctcaatcc
     4441 tccggcaata tttagaaaga catccttggc tgcaagccta aatccggcac gtttctccag
     4501 tacggcaagc agcatattga gcctgcgcac atcaaatccg gtggttgagc gttgaggagt
     4561 accgtaggct gccgtactga ccaatgattg tatctcaatg agaaatggtc ttgtaccatc
     4621 catcatggca ctaactgcca ccccgctcaa atcatctttg tgcataggca taagaatttc
     4681 ggatggattg gagacttctc taagaccgct gccggtcatc tcaaaaactg caagctcatc
     4741 ggtggatccg aaacggtttt ttatactgcg gagtatacgg taggcaccct ttgtatcgcc
     4801 ctcgaaaagc agcactacat ccactatgtg ctccagaatc ttcggacccg caatagcgcc
     4861 atcctttgtt atatgtccta tcaggatcac cggcacattg ctctctttgg cgtagcgcag
     4921 caatactgag gcgcactccc taacctgtga aacgctgccg gcagaggatt caatgctttg
     4981 actatagata gtttgaatgg agtctataat gagcaaagag ggattcactt cccgggcctg
     5041 taacagtatg ttttccagca gagtttcccc cagtacgtag cattgggagt catctccctg
     5101 taatctttga gcgcggagtt tgatttgtac tggactctcc tcccctgata catatagggt
     5161 cttgaggcct tcagcatgga gcggaatctg taaagagaga gtggactttc cgatgccggg
     5221 ctcacctcca attagtatca tggagccttg taccataccc cctcccagta tcctgtccag
     5281 ttccccattt cctatgaaaa atctctgttc ctcgtcaaag gtaattccgg tcagcagctg
     5341 aggacgcgct tcctgcgttt gcaggaaaga acggttacgt ccggaggctc cacccccggt
     5401 accggaaggg gcttttggct cctctatcat agtgttccat tcgccacaag cagggcagcg
     5461 ccccatccat tttggacttt cattaccgca ggaactgcag aaccatgctg attttgtctt
     5521 tgccatgatg caaagataat aaatcactaa aaaatctcga acatacctca atattatctg
     5581 acgaataatt atatttttcc tatttttgcg aacaaaccaa aaacctcaaa aaaagtgatc
     5641 aatttcaata tcgaatacaa gacccatttc ggtcaacaat tatatgttgc ggggtcaatc
     5701 cccgaactgg gagagtggga ttatttccgt gcgcttccta tggcatattc tgatggggga
     5761 aattggaagg ctaatatcaa aaccccttca ggcacttttt catacaaata tatattaaag
     5821 agtccttcgg gtattttggt tgaggtgggt gaacccagaa ctatttccgc aggcggtcgc
     5881 tcaggtaata tttcactata cgatatgtgg cagggcagca gtgaccattc cgcttttctt
     5941 tcagctccgt ttgaaaatgt tttttacagt agaaagagcc tacagactcc ggttgaaacg
     6001 gactttgcga aggaagtggt tatcagtgtt acagcaccat tggtacactc agacgactct
     6061 atttccatct gcggagagtg cgatgcactt ggcaattgga atccgcttaa ggctttgcct
     6121 atgcgccctc tccccgggtg caaatgggag gttgctctgg atgcatccct actgcctaaa
     6181 ttggtgaggt tcaaatttat caaaattata ggagatactg cc
//
LOCUS       DAOSIE010000064         6205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_11430_length_6205_cov_4.307642, whole genome shotgun sequence.
ACCESSION   DAOSIE010000064 DAOSIE010000000
VERSION     DAOSIE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11430_length_6205_cov_4.307642"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(461..955)
                     /locus_tag="PLI69_03090"
     CDS             complement(461..955)
                     /locus_tag="PLI69_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HOG41506.1"
                     /translation="MKDFAAIDFETANECRSSVCSVGVVVVRGGEIVERFYSLIRPEP
                     EYHKWFCQQVHGLSEEETSDAPVFPYVWEKIAPMIEGLPLVAHNSHFDEGCLKEVFRV
                     YQMDYPDYVFYDTCATSRKHFANSIDNHELQTVAAACGYNLENHHHALADAEACAHIA
                     MKIL"
     gene            complement(958..1863)
                     /locus_tag="PLI69_03095"
     CDS             complement(958..1863)
                     /locus_tag="PLI69_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOG41507.1"
                     /translation="MALNYFNRYIWLIEVINRHGHISRKDISDLWARSQLNELGESYL
                     PERTFHNHISSIFDTFGIEIKCDRSLGYYIANSDDIKGDEIRKWLMQSLSLNNLLNES
                     HNMRDSIIFEEVPSSEKYLSAIIEAIRDRKAIEITHQGFTKEYSTTFEAEPYCLRQFK
                     QRWYMVAKTAASDKPRIYGLDRIKELRRIGRSYKIPKKFTAKSYFKDLYGACIEDSKY
                     AEEVEIKVDKPQVHYFNSLKLHESQTPIEETSDYTIFRYHIVPTYDFTQELLSKSDSI
                     EVLKPQWYRNEIKNTIKKMLKRYKD"
     gene            complement(1949..3736)
                     /locus_tag="PLI69_03100"
     CDS             complement(1949..3736)
                     /locus_tag="PLI69_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG41508.1"
                     /translation="MEKKISTKEILAGMLGCQELIDDLGLELKNKTLLECFEEVIERA
                     RRDREYSRDYGDYFSLDDKLIAAKRLELETIAEEYGISEWQAALFAIVIELCKGEDIR
                     QGQLVERLKTSFISYLSIEKDLRELEKKFLIKINNRDCISVSNEVIDLLRENKPFRKP
                     SYEGLSTGIILSKMNRLFNDYDEGNIKLLLEELDNMILANPATSIAKTADSYRILIEG
                     DKIIYPEITNDYLNSMNKNERMLFYALIHRYDHYNDDECEWWDFRDFFNDYEFDSIHA
                     NFKENKLALQRMEVIEYANIDGMSVKDRFRLKDEVKEKIYADVGGLHESTPMAGLMEY
                     GTITQKSLFYDQDVQKQVKTLESILSAERFNEVTKMMKSKGMRNGFTCLFYGAPGTGK
                     TETVYQLAKRTGRDLITADVTKIKSYWVGESEKNIKELFRKYKRCVKESKVAPILLFN
                     EADAIFGIRKEGADRAVEKMENSIQNIILQEMESLDGILIATTNMTQNLDKAFERRFL
                     YKIKFDVPSVEVKSKIWHSMVPDLDEEQTRKLASMFNFSGGQIENVSRKKMVQSIITG
                     TEPSYEEMVEFCREEMIGNNRQTGNRIGF"
     gene            4004..4162
                     /locus_tag="PLI69_03105"
     CDS             4004..4162
                     /locus_tag="PLI69_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41509.1"
                     /translation="MAKPIKETPLLTGEDATRFEQAAQEVVPASEKEINEAREAFDYF
                     ASIATFSM"
     gene            complement(4299..4610)
                     /locus_tag="PLI69_03110"
     CDS             complement(4299..4610)
                     /locus_tag="PLI69_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008564330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG41510.1"
                     /translation="MRPKIDTDKLKHLPTAEEMFVNEYGAKGTASRDEFDAKSRAWYY
                     AEVLKNARKSAGITQQQLADKIGKKREYIAMLEKGETDMQLSTFIMIADAVGLKLALT
                     Y"
     gene            complement(4610..4939)
                     /locus_tag="PLI69_03115"
     CDS             complement(4610..4939)
                     /locus_tag="PLI69_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG41511.1"
                     /translation="MRTIVRSIEFDEFYNSLPVNVQNKVQYALNIISEVKVVHTKLVK
                     KLVDTDFYELRISVSNEYRVILFTIDHESFVEAQQILLLNGFMKKSTKDYKKEIKKAQ
                     QILNTLQ"
     gene            5154..5405
                     /locus_tag="PLI69_03120"
     CDS             5154..5405
                     /locus_tag="PLI69_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41512.1"
                     /translation="MAKFYCKYCGQEFGNVRTLTSAYCMRHPDGPNKGKHTLYEGSEK
                     SQYTCKFCGQQFNNLRSLTSAYCLRHPDGANKGRHSPAL"
     gene            5456..>6205
                     /locus_tag="PLI69_03125"
     CDS             5456..>6205
                     /locus_tag="PLI69_03125"
                     /inference="COORDINATES: protein motif:HMM:NF039481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HOG41513.1"
                     /translation="MKKNAPWWLSHKPIINVEYEGLDAEAGDAKFITIGKATWNKKDY
                     SAKVWRQVYETGRWSRQSEELPLWRVLDLATLLVATINNKQSNLGEYFQDQESELELR
                     SYLEENMEVLGPKMNQLSEMLKQTPIDISSEGTPNIFSFATSELSQDAMFAWLIRWAS
                     PEYKKSDSDLHNIAQDFVRLLMNKDSSFEILSVNVGRQLYNIDVWAEINENSFLAIED
                     KTGTSIHDNQLTRYKQIAENKYFGKRTDLCFA"
BASE COUNT         1742 a         1314 c         1226 g         1923 t
ORIGIN      
        1 atcagctggt tctcgacaag ggcttcctca caaaagagca gcttgacaaa attatggacc
       61 cggccaatat gaccaaaccg gttaaggtgg agatataagt ggtgcggggt gcgagttacg
      121 cgttacgagg ttcagtgtat gttctggggt gcggggtacg agttgctcgt ttaatgttgc
      181 gaggttcgtg gtgtaaggag tgaggtgttc tgagttccga gtacgctagt acagagtaaa
      241 ttacaagaat agtccggtgt ttgccggact atttttgtta tcttagtttt gagtagtacg
      301 ggttgtgaat tgtgaggtgg gaggggttct gactcgagag ttaagagcga ctcaacctaa
      361 aagaatccgg tagttagaac aaattcgccg cgaacctcac gggcggcggc gaatacaaac
      421 tctaacaatt taactttaat actatgatga gaaaaattct ttataaaatc ttcatggcaa
      481 tgtgcgcaca ggcttctgca tcggccaggg cgtggtgatg gttttcaaga ttgtatccgc
      541 aagcagcggc aaccgtctgt aattcgtgat tatctatgga gtttgcgaaa tgctttctgg
      601 aggtagcgca ggtgtcgtaa aacacatagt ccggataatc catctgatat accctgaaga
      661 cctctttaag acatccctca tcaaaatgac tgttatgagc cacaagcggc aggccctcaa
      721 tcatcggcgc aatcttctcc cacacataag ggaataccgg cgcatcggat gtctcctctt
      781 ccgaaagacc gtgaacctgt tgacagaacc atttatggta ttcaggttcc ggacggataa
      841 gactgtagaa tctctccact atttcacctc cgcgaaccac aaccactccg acggagcaga
      901 cgctgcttcg gcactcattg gccgtttcaa aatctattgc tgcaaaatct ttcataatta
      961 atctttgtac ctcttcaaca ttttcttaat ggtattcttg atttcattgc ggtaccattg
     1021 aggcttcagg acttcgattg agtctgactt ggaaagtagc tcctgcgtga agtcgtaagt
     1081 gggcactatg tgatatctga atatggtata atccgaggtc tcctcgatag gagtctgcga
     1141 ttcgtgtagc ttgagggagt tgaagtaatg gacctgtggt ttatcgacct tgatttcaac
     1201 ctcttcggca tacttgctgt cctcaatgca ggcaccgtaa agatccttga aatagctttt
     1261 cgccgtgaat ttctttggaa tcttatagga gcgaccgata cggcggagtt ccttgatacg
     1321 gtcgagacca tagatcctcg gcttgtcgct tgcggcagtc ttggccacca tataccagcg
     1381 ctgcttgaat tgtcgcaggc agtatggttc tgcttcaaaa gtagtggaat actccttggt
     1441 aaatccctga tgtgtaattt caatcgcttt tctgtcgcgg attgcctcta tgattgcaga
     1501 gagatacttt tcgctcgaag gcacctcctc gaagatgatg ctgtcccgca tattgtggct
     1561 ttcgttcagc aggttgttga gcgagagcga ctgcatgagc cattttctga tttcatcacc
     1621 ttttatatca tcgctatttg caatataata ccccaggctc ctgtcgcact tgatttcgat
     1681 gccgaaagta tcgaagatgg aactaatgtg gttgtggaat gttctttccg gaaggtaact
     1741 ttctccaagc tcatttagtt ggctgcgtgc ccaaaggtcg cttatatcct ttctggagat
     1801 gtgcccatga cggttaatga cctctatgag ccagatgtag cggttgaagt aattaagtgc
     1861 catgcctttt ggtttttgct ttcaatgaca aagataatta attaaactgc caaaaagtag
     1921 cagcctgctt atttttggca gaattttatc aaaatcctat tctatttccg gtttgcctgt
     1981 tgttgcctat catctcctct ctgcaaaatt ccaccatctc ctcataggaa ggctcggtgc
     2041 cggtgatgat tgattgcacc atcttcttgc ggctgacatt ttcaatctgg cctccggaaa
     2101 aattgaacat tgaggcgagt ttgcgcgtct gctcttcatc gagatcggga accatcgagt
     2161 gccaaatctt ggattttact tcaactgaag gtacatcgaa cttgatttta tagaggaaac
     2221 gtctctcgaa agctttatcc agattttgcg tcatgttggt ggtggcgata agaatgccgt
     2281 cgagactctc catctcctgc aggatgatgt tttggatgga gttttccatc ttttcaacgg
     2341 cgcggtctgc accttccttt cgtattccga atattgcatc tgcttcgtta aaaagcagaa
     2401 tgggcgcaac tttggactct tttacacatc ttttgtattt tctgaatagc tcttttatat
     2461 ttttctcact ttctccgacc caataactct taatcttagt gacatcggca gttatcagat
     2521 cccgaccggt gcgtttggca agttgataca cggtctccgt ttttcccgta ccgggtgccc
     2581 catagaatag acaagtgaag ccgtttcgca tacccttgga cttcatcatt ttcgttacct
     2641 cattaaatct ttctgcagat aaaatgctct caagtgtttt tacctgtttt tggacatcct
     2701 gatcataaaa tagtgatttt tgggtaattg ttccatactc catcaggcct gccatcggag
     2761 tactttcatg caatccaccc acatcagcat agattttttc cttgacctca tcttttaggc
     2821 ggaatcggtc tttcacagac ataccgtcaa tatttgcata ctcgataacc tccatcctct
     2881 gaagggccag tttgttctct ttaaaatttg catgaataga atcaaactca tagtcgttaa
     2941 agaaatccct aaaatcccac cactcgcact catcatcatt atagtgatcg tatcgatgaa
     3001 tcaaagcata gaaaagcatc cgctcattct tattcattga atttaaatag tcattagtaa
     3061 tctcagggta aataatttta tccccctcta ttaaaatacg atatgaatcg gctgtcttgg
     3121 caatggaagt agcggggttg gccaggatca tattatccaa ttcttcaagc aacaatttga
     3181 tatttccctc atcataatca ttaaataaac gattcatttt cgaaagaatt atacctgtgc
     3241 tcaatccttc gtaagatggc ttacggaagg gcttattttc acgaagcaga tcaataacct
     3301 catttgacac agatatacag tcgcgattgt ttattttaat caaaaacttc ttctccaact
     3361 cacgtaaatc tttctctatc gagagataag agataaaact tgtcttgagc cgctcgacaa
     3421 gctgcccctg cctgatatcc tcccccttgc acaactcaat cacaatggca aacaacgctg
     3481 cctgccactc gcttattcca tattcttcgg caattgtctc caactctaac cgtttagcgg
     3541 caatgagctt atcgtcaaga gagaagtaat caccgtagtc ccgactatac tccctatccc
     3601 ttcgcgctct ctcaataacc tcctcaaaac actccagcaa agtcttattt ttcaattcca
     3661 gacccaaatc atcaatcagc tcttggcacc ccagcatacc cgccaggatc tcttttgtag
     3721 atattttctt ttccataact atgctccttt tttacttttg ttattgctgt atgttgctta
     3781 cttgtgcttg cagcttatct gctattgcca cagcttactt tgcttactgt agcaaaatgc
     3841 tttttcacta ctgcaaagaa aataaatctc gctgccaaaa agtggcagtt cttaggaatt
     3901 ttggttattt tctgaatttg gtgttcaaaa ttgtcttttc tgcacatctc gttgatattt
     3961 gtttcatatc tttgtggaga aattgataaa aaagaaaaag attatggcaa aaccaattaa
     4021 agaaacacct ctccttaccg gtgaagatgc aactcgtttt gaacaagctg cacaagaggt
     4081 tgtgcccgca tctgaaaaag aaataaatga ggcacgcgag gcgtttgatt attttgcttc
     4141 aatagcaacc tttagtatgt aatgggtcct cagttaagaa aattaactcc ggaatataac
     4201 aacacccttt gatttgtagt caaagggtgt tgtgctaatt ataaaacctt aagaactaat
     4261 agatgacggc taaaagccat tagcaaattt actacttatc aataggtcaa ggctaatttt
     4321 aagccgacag catcagcaat cattataaat gtagaaagct gcatatcggt ttcgcctttt
     4381 tcgagcatag caatatactc acgcttctta ccaatcttgt cggcaagttg ctgttgggta
     4441 attccggccg atttacgggc atttttcaac acctctgcat agtaccaagc acgagacttc
     4501 gcatcgaact catcacgcga agcggttcct tttgctccgt actcatttac aaacatttcc
     4561 tcggcggtgg gaagatgttt gagtttgtct gtgtcaatct ttggtctcat tactgtaaag
     4621 tatttaatat ttgttgtgct tttttaatct ctttcttata atccttagtg gactttttca
     4681 taaaaccatt caaaagaagt atctgctggg cttctacaaa actttcatga tcaatggtaa
     4741 ataggataac cctatattca ttacttacag atatacgtaa ttcataaaaa tccgtatcca
     4801 caagcttctt taccaactta gtatgtacaa ctttgacttc tgaaataatg ttcaatgcat
     4861 attgcacctt attttgaaca tttacaggaa gcgagttgta aaactcatca aattctatac
     4921 ttcttacgat tgttctcatg tacaaatgta atatatttat tacgtttttg caaaaaatgt
     4981 caaataatgt caaaaaatat caaataatgc cctgtggcac attaataata ttgtattacc
     5041 caaatactgc cggactttag cagaatggag cattatattt gcggtatcag cttgaaaata
     5101 aataactatc tttgtttgca agctttcagg ttcaactaca aataattata tccatggcca
     5161 aattttactg caaatattgc ggacaagagt tcggcaatgt gcgcacatta acttctgctt
     5221 attgtatgag acatccggac ggtccaaaca aagggaaaca cactctttac gaaggttctg
     5281 agaagagcca atacacctgt aagttttgtg gtcaacaatt caacaatctt cgttccctaa
     5341 catctgccta ttgcctacgc cacccggacg gagccaacaa aggcagacac tcaccggcgt
     5401 tgtaaccgtt aagatttcta tagatttatt atttaattat taaaatgaca caaaaatgaa
     5461 aaagaatgct ccttggtggc tttcacataa gccaataatt aacgttgagt atgaaggtct
     5521 tgatgctgaa gctggagatg caaaattcat cacaattgga aaggcaacgt ggaataagaa
     5581 ggactattct gctaaagtat ggaggcaggt atatgaaaca gggcgctggt ctaggcaaag
     5641 cgaagaatta ccactttggc gtgtgcttga tcttgcaaca ttgcttgtag caacaataaa
     5701 caacaagcag agtaatttag gggaatattt tcaagaccaa gaatctgaac tagaactcag
     5761 aagctatctt gaagagaaca tggaggtact aggtcccaaa atgaatcaat tgagcgagat
     5821 gcttaagcaa acaccaattg atatttcttc tgaaggtact ccaaacattt tctcttttgc
     5881 tacatctgaa ctcagccaag atgctatgtt cgcttggctt atcagatggg catccccaga
     5941 gtataaaaaa tctgattctg acttgcacaa tatagcgcag gattttgtca gattactgat
     6001 gaataaagat agttcatttg agatattaag tgtgaatgtt ggcaggcaat tgtataatat
     6061 tgatgtatgg gcggagatta atgaaaattc attccttgct atagaagata aaaccggaac
     6121 atcaattcac gataatcaac tcactagata taagcaaatt gccgagaata aatattttgg
     6181 taagagaaca gatttgtgtt ttgct
//
LOCUS       DAOSIE010000065         6127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_11604_length_6127_cov_3.612978, whole genome shotgun sequence.
ACCESSION   DAOSIE010000065 DAOSIE010000000
VERSION     DAOSIE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11604_length_6127_cov_3.612978"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PLI69_03130"
     CDS             <1..843
                     /locus_tag="PLI69_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOG41514.1"
                     /translation="QLRKCGVEWFDFYLMHNIHETCIGIYLDPKWGILEYFLKQKEMG
                     RIRHLGFSAHAELETLKRFLDSPYGEHMEFCQIQLNFLDWSLQRAREKVELLNSRDIP
                     IWVMEPLRGGKLLSIATPGEAFRWVAGILGVTMILSGMSNEQQMIENIEIFNRMAPLS
                     QEECDRLYALAEQMKESVPCTACNYCTPTCPMGLDIPTLIQARNDFAIETTFTPLMRI
                     ESLPKEGHPSNCIGCGACSQMCPQGIDIPEVISELNSQLAKAPSWKEICRQREIAARK
                     MREG"
     gene            933..2537
                     /locus_tag="PLI69_03135"
     CDS             933..2537
                     /locus_tag="PLI69_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG41515.1"
                     /translation="MKVKKLLLGDEAVALGAINAGITGVYAYPGTPSTEITEYIQESE
                     TARKAGIHSTWCTNEKTAMETALGVSYVGQRALVCMKHVGLNVAADAFVNSAITGVNG
                     GVIVVVADDPSMHSSQNEQDSRFYGKFAFIPILEPSDQQEAYDMMEYGFRLSEENNIP
                     VMMRLTTRMAHSRAVVEVDKSPTPRKKELSFPKDPSSWILLPANTRHKYLKVISGYEE
                     LERIGVSKEGWNRFFDAPDKSLGVIACGIAYNYLRENVGMNYQHPILKISQYPAPSDL
                     IRKMASECNSILVIEEGQPLLEEQIKGILPTPVKVMGRLSGELPRVGELNPNSVRAAL
                     GLPAHDIHQPSSVVVPRPPALCQGCGHRDVYTALNEVLADYPNHKVFSDIGCYTLGAL
                     PPFRAISSCIDMGSSITVARGAADAGLWPSIAVIGDSTFTHSGITSLLDAANVNANIV
                     VVISDNLTTGMTGGQDSAGTGKLEAICAAVGVDPAHIRTVIPLRKNMEEITRIIREEI
                     KYEGLSVIIPRRECIQTAKRNKEKKQ"
     gene            2534..3106
                     /locus_tag="PLI69_03140"
     CDS             2534..3106
                     /locus_tag="PLI69_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOG41516.1"
                     /translation="MKKDIILAGVGGQGILSIAAVIGEAALKEGLHIKQAEVHGMSQR
                     GGDVQSNLRISSNPIASDLIPKGCADIIISLEPMEALRYLPWLSKEGWIVSSTTPFVN
                     IPNYPSTESIMEEFQKINNKIMVDVESIAREVGNPRGANIVLLGACSHLLGMEAQKFE
                     EGIRRIFSNKGEDIVEANIKAFKAGQNAAF"
     gene            3137..4561
                     /locus_tag="PLI69_03145"
     CDS             3137..4561
                     /locus_tag="PLI69_03145"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HOG41517.1"
                     /translation="MKKILTLLLAVAVTTTAMGQGRLGGLLNKAKEAVSSEAKSDNKE
                     NRNNTIFSSGNAIHVSGLTGSNRNDGSKAKPYKNLQKAIDNAKPGDVILVAQGNYFGM
                     LNSGNIMIDKPISIYGGYSDDFSTRDILKYRTMVQPDPTSNGTAKGGGTIQIKGTSGG
                     EVVIDGLLIDRGNSIAYNPAGKGKPEGVESPMMQPIGTAGIGGASFQEKVHTTETALI
                     YLDNPTYDIKIRNTAFVNSPNYGIRGMFKGKMEVDNCIFINIRMAAVEVSGSYATEYC
                     EVKFTNNTVLFTWSRLKDMADMGYGYRYMNGVHSYLDHNIIGCSIFSGLDRCRIDSNA
                     AREREKITTCENSLFFLNKQADICLPGGGMFLRVWVKDFDDVEQLAEVSGNKSVSDPS
                     IFNGALNEPYLKGFIEAAYNERTNFDPNSGANVFRQAMGMNMQGTIDSSASMFANRYP
                     FEDALKFFGAVEGYGAQIPSKAKK"
     gene            complement(4661..>6127)
                     /locus_tag="PLI69_03150"
     CDS             complement(4661..>6127)
                     /locus_tag="PLI69_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41518.1"
                     /translation="EDEIDIVPPAEGDVPVVISPEEKAANDLRLAHEDSLRNSYVSTF
                     VKPSAKDPENSFLVASRGNWREISSYLEQIKSGSKPRYRDGLRLLSIISEKDLRDTPA
                     DVLLDHLNNFSGVEGMYPQQQEIWYRYVLNPRIGNELLKSWRREFRTIELSFTLSSGR
                     DIAAYVRNNIKVLDRYNPQRIPITPLGVHSLKAADSFSRDCYFVALCRALNIPARIEE
                     ISGKVQYYDSGYWIDAAAELSSEIVGESEVKRPSGFLMIQDSPLDNIDDPKFESHFTI
                     ASLEGGRINTLNFRDKEGIEGTMSVRNVFKEPVELDCGSYLLTTGTRMASGKVLSKMH
                     FFNIEEGGTTTIDLRLREDDDDLQVLGNFYADPLLPLTGRGIYILAFVRSSHEPSDHI
                     IRALFAHDWSIPVILFFKSELEMSRLNMDMFPQIPHKVSFATDDNNILTAICDAMKLD
                     SRHLKMPFVVMADSFGRIFLTYDGYSTTLLDQLLKAER"
BASE COUNT         1740 a         1422 c         1464 g         1501 t
ORIGIN      
        1 cagttgcgta agtgcggtgt ggagtggttt gatttctacc ttatgcataa tatacatgag
       61 acctgtatag gtatctatct tgaccctaag tggggtattc tggaatattt cctgaagcag
      121 aaggagatgg gtcgtatccg tcatctcggt ttttcagcgc atgcggagct tgaaacactc
      181 aagcgttttc tcgactctcc ctatggagag catatggagt tttgtcaaat acagctcaat
      241 ttcctggatt ggtcactcca gagagcgcgg gaaaaggtag aactgctcaa ttcgagagat
      301 attcccattt gggttatgga accattacgc ggcggtaagc tcctctccat tgccacacct
      361 ggggaggcct tcagatgggt ggccggtatt ctcggggtca cgatgatcct cagcggcatg
      421 tcaaacgagc aacagatgat agagaatatt gagatattca accgtatggc accgctcagt
      481 caagaagagt gcgaccgtct ctatgctctt gctgagcaaa tgaaagaatc ggtgccctgc
      541 acagcctgca actattgtac tccgacctgc cctatgggac tggatatccc cactctgatc
      601 caggcgcgca acgatttcgc cattgaaacc accttcactc ctctaatgcg catcgaatcg
      661 cttcctaaag aaggtcaccc ttcaaactgc ataggatgcg gtgcctgcag tcaaatgtgc
      721 cctcaaggca tagacattcc tgaagtaatc agcgaactca actcacagct tgccaaagcc
      781 ccttcctgga aagaaatttg tcgccagaga gaaatagctg cccgcaaaat gcgggaagga
      841 tgaattaaat atgtaagcat tttgaggatt ttattccaat gttatattgc atttttgcta
      901 tatttgaaga ttgaatgaaa aaataataga taatgaaagt taaaaaattg cttttgggtg
      961 atgaagccgt ggcgctggga gcgataaatg ccggtattac cggagtttat gcttacccgg
     1021 gtaccccatc gaccgaaatt acggagtaca ttcaggagag tgagacggca cgcaaagccg
     1081 gtatccacag cacatggtgt accaatgaga agacagcgat ggagacagcc cttggtgtct
     1141 cttatgttgg acagcgcgcc cttgtatgta tgaagcacgt aggtttgaat gtagctgcgg
     1201 atgccttcgt taattccgca attacaggcg tcaatggagg agtgatagtg gtggttgcgg
     1261 acgacccttc gatgcactct tctcagaatg agcaggacag ccgcttttat gggaaatttg
     1321 cctttattcc catactggag ccctccgacc aacaggaggc ctacgatatg atggagtatg
     1381 gctttcgtct ctcggaggag aacaatatcc ctgtgatgat gagacttacc accagaatgg
     1441 ctcactcgag ggcagtggtt gaggtggata agagtcccac cccccgcaaa aaagaactct
     1501 catttcccaa agacccctct tcgtggatat tgcttcctgc caatacgcgc cacaaatatc
     1561 ttaaggtaat atccggttat gaagaactgg agcgaatagg cgtatcgaag gagggttgga
     1621 accgtttttt cgacgctccc gacaagagcc tcggagtgat tgcttgtgga attgcataca
     1681 actatcttag agagaatgtc gggatgaact atcaacatcc gattctaaag atttcacaat
     1741 atccggcacc gtctgatctg attcgcaaaa tggcctcaga atgcaactcg atccttgtca
     1801 ttgaggaggg acagccgctg cttgaagagc agataaaagg tatcctaccc actccggtta
     1861 aagtgatggg acgcttaagt ggagagctgc caagggttgg ggaactcaat ccgaactcag
     1921 tcagagcggc tcttggactg ccggcacatg atattcacca gccgagcagc gtggtagtgc
     1981 caaggcctcc cgcactttgc cagggttgcg ggcatagaga tgtttatacc gcactcaacg
     2041 aagtgttggc ggattaccct aatcacaagg tcttcagcga tataggctgc tataccctcg
     2101 gagctctgcc cccatttagg gccatcagtt cctgtatcga tatgggatcc tccataacgg
     2161 tcgccagagg agctgcagat gccggtttat ggccaagcat agcggtcata ggcgactcca
     2221 cttttaccca ctcgggcatc acaagtctgc ttgatgcggc caatgtcaat gccaatatcg
     2281 tggtggtaat ctcagataac ctcaccaccg gtatgaccgg aggacaggat tctgcgggta
     2341 caggtaagtt ggaggctata tgcgctgccg taggagttga cccagcccac attcgtaccg
     2401 ttataccttt gcgcaagaat atggaggaga tcacgcgaat cattcgcgaa gagataaaat
     2461 acgaggggct ttcggtaatt attccccgga gggaatgcat tcagactgct aaacgaaaca
     2521 aagagaagaa acaatgaaaa aagatataat acttgccggc gtgggaggac aaggtatcct
     2581 ctccatcgcc gccgtaatag gagaagcagc cctcaaagag ggtttgcaca tcaaacaagc
     2641 tgaagtccac ggtatgagcc agagaggtgg cgacgtgcag tccaacctac gcattagttc
     2701 caacccgatt gcttcggatc tgattccgaa aggttgtgcg gacatcatca tttcgcttga
     2761 gccgatggag gcgctaagat acctcccctg gctctccaaa gagggttgga ttgtaagcag
     2821 taccacgccg tttgtcaata ttcccaacta tccctctaca gagtctataa tggaggaatt
     2881 ccaaaaaata aacaacaaaa taatggtgga tgtggagagt attgcacgtg aggtgggcaa
     2941 cccccgtgga gccaacattg tcctccttgg agcatgcagt caccttttgg gtatggaagc
     3001 tcaaaaattt gaggagggta ttcgcagaat attttccaac aagggagaag atatcgtgga
     3061 ggctaacatt aaagctttca aggcaggcca aaatgccgca ttttaataaa aaatccaatt
     3121 ttaattaaat acatttatga aaaaaatttt gacactactc cttgcagttg cagtcactac
     3181 aactgcaatg gggcaaggca gactgggcgg tttgctcaac aaagccaaag aagccgtttc
     3241 aagcgaagca aaatccgaca acaaagagaa cagaaacaac actattttca gtagtggaaa
     3301 tgctatccat gttagtgggc tgacaggctc aaacagaaat gacggcagca aggctaagcc
     3361 atacaagaat ctccagaaag cgatagacaa tgcaaagcct ggggacgtta ttcttgtggc
     3421 acaaggcaat tatttcggaa tgctcaactc cggcaatata atgattgaca aacctataag
     3481 catctatgga ggctattccg atgatttctc tactcgagat attttgaaat atcgcaccat
     3541 ggtgcagccg gatcccacat ccaatggtac cgcaaagggt ggcggaacaa tccagatcaa
     3601 aggtacaagt ggtggtgaag tggttattga cggcctactt attgacagag gtaactccat
     3661 tgcctacaat cctgccggca aaggcaagcc tgaaggagta gaaagcccga tgatgcagcc
     3721 tatcggcacg gcgggcatcg gcggagcaag cttccaagag aaagtacata ccaccgagac
     3781 agctctcata tatctcgaca accccactta tgatataaag atccgcaaca ccgcattcgt
     3841 caatagcccc aactatggta taaggggtat gttcaaaggc aaaatggagg ttgacaactg
     3901 cattttcatc aatatccgta tggctgccgt agaggtatca ggaagctatg caacagagta
     3961 ttgtgaggta aaattcacaa ataacacagt ccttttcacc tggtcgcgtc ttaaagatat
     4021 ggctgatatg ggttacggct atcgttatat gaacggtgta cacagctacc tggatcacaa
     4081 tattatcggg tgcagcattt tctcgggact tgaccgctgc cgcattgaca gcaatgccgc
     4141 cagggaacgt gagaagataa cgacttgcga aaactctctt ttcttcctca acaaacaggc
     4201 tgacatctgt ctccctggag gcggtatgtt cctaagagta tgggtaaagg actttgacga
     4261 cgtggagcaa ttggcagagg tttcaggcaa caaatccgta tccgatccct caattttcaa
     4321 cggtgccctc aatgaaccct acctcaaagg ctttatcgaa gccgcatata atgagaggac
     4381 caatttcgat cccaactcag gtgccaatgt tttccgccag gcaatgggaa tgaatatgca
     4441 gggtaccata gactcttccg cttccatgtt tgcaaataga tatcccttcg aagatgcact
     4501 aaaattcttc ggtgctgttg agggttacgg agcacaaatt ccgagtaaag cgaagaaata
     4561 aacaaaagaa ataagcaggt tgagccccga aagttttttt actttcgggg tttttgttac
     4621 aaccgatacg taacccgcaa cacgaaccgc acaacacaaa ttatctttct gcttttaaaa
     4681 gctgatcaag tagggtggtc gaatagccgt cgtaggtcag gaaaattctt ccgaagctat
     4741 ccgccatcac cacaaaaggc attttgagat gcctggagtc cagtttcatc gcatcgcaaa
     4801 tggctgtcaa aatgttgttg tcatcggtgg caaaggagac cttgtgaggt atctgaggaa
     4861 acatatccat atttaatcgg gacatttcca attcagactt aaaaaataat atcacaggga
     4921 tactccaatc gtgagcaaag agtgcacgaa taatatggtc acttggttca tgggaggacc
     4981 ttacaaatgc aagaatatag attcccctgc cggttagcgg caacaaagga tcagcgtaaa
     5041 aattgccaag cacctgcaaa tcgtcatcat cttctcttaa acgaagatcg atggtagtcg
     5101 taccaccctc ctctatattg aagaaatgca ttttggagag aaccttaccg ctggccattc
     5161 gcgtgcccgt ggtaagcaaa taactgccac aatctagctc taccggctct ttgaagacat
     5221 ttcgtacact cattgtcccc tctatgccct ctttgtcacg gaagtttaga gtatttatgc
     5281 ggccgccctc aagcgaggct atagtaaaat gcgattcaaa tttgggatcg tcaatattgt
     5341 caagaggact atcttgtatc atcaggaagc cggaaggcct cttaacctca gattcaccca
     5401 caatttcaga tgagagctca gcagccgcat ctatccaata tccggaatca tagtactgta
     5461 ccttaccgct tatctcctct atccttgcag ggatgttgag agcgcgacag agggccacga
     5521 aataacaatc gcgcgaaaaa ctgtcggcgg cttttaggga atgtactccc aagggagtaa
     5581 ttgggatgcg ctggggatta tagcgatcca gaaccttgat gttatttcgc acataagcgg
     5641 caatatccct gcccgaactc agagtaaatg acagttcgat ggtccggaat tctcttctcc
     5701 agctcttaag caattcattg ccaatgcggg gatttaacac gtaacgatac catatctcct
     5761 gctgctgcgg atacattccc tccactcctg aaaagttatt caggtgatcg agaagtacgt
     5821 cagcaggcgt atcacgcagg tctttttcac ttataatgct aagtaaacgc aaaccgtcgc
     5881 gataacgggg tttactaccc gatttaatct gctccagata agaggagatt tccctccaat
     5941 tgcctctgga tgccaccaaa aagctatttt cgggatcttt ggccgatggc ttgacaaagg
     6001 tagagacata actgttgcga agggaatcct catgagcaag acgcagatcg ttggctgctt
     6061 tctcttcggg agatatcacg acaggaacat ctccttcagc cggaggtaca atatctattt
     6121 catcttc
//
LOCUS       DAOSIE010000066         6109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_11649_length_6109_cov_4.032871, whole genome shotgun sequence.
ACCESSION   DAOSIE010000066 DAOSIE010000000
VERSION     DAOSIE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11649_length_6109_cov_4.032871"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..908)
                     /locus_tag="PLI69_03155"
     CDS             complement(267..908)
                     /locus_tag="PLI69_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOG41519.1"
                     /translation="MKSFKSFFNPTYGYLRALVGIVAGLVLLIWPDTMKDLIVRIIGG
                     LFLVIGCVSLVIYFISRRKSIKSQATTDRVKKNFFVVNAILSIAFGAVLILIPDVFVG
                     FLLLLLGIILVVFGIGEIVTFIAAHKYAPIPWYMYVLPTIVALCGLFLVFNPHESQIL
                     MFQLLGAGFLGYGLFELYATIKIRKAIKEKLSESYANPAKELVEDVDYEEIKE"
     gene            complement(934..6072)
                     /locus_tag="PLI69_03160"
     CDS             complement(934..6072)
                     /locus_tag="PLI69_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOG41520.1"
                     /translation="MIQITPKREFSVSLIDERTVALFFAEPLDFNAEYKVQIDAGKLV
                     GRRSAKVTYNFKTLAPEIHYELGAITVYPDEEDKYHLDIVITTSDPVGNEYIEKGISL
                     SKDFPVSWQHAEDGLVHRLSINNIIPEKKQSVFTISRKYELFNDMGSWSFNIPAEGAF
                     VIISAEPLLDPYRYEIVFSSALDKKQVMANLVKLPNAGKLKFLVDRNKLTIYPSIEDV
                     LRQTVNISKLVKNAKGEQLGFDWEQTFDVPQKKPFIKFSTKGVVLPSSNGNKLFFQSQ
                     NYAKVQVRVHRIYENNMLQYLQSERLDEKSNYILGRVAKVVVDTVLVLADPSSSKLKS
                     VASYGLNITDLIKVQKGALYRIGIKGVDPLVEQDSDYYDSDYYFGSYYDYEERHVNIL
                     ASDLAVIAKGSGKGTYTFFVTNIIDASPVSGAKVRVFDYVNQVIGEGSTGSDGKVEIK
                     LSEEPHTAVVTSGSDKNYLKLNYGASLSVSNFDVGGTSVSGGQKGYIFGERGVWRPGD
                     DIYITFISMLEEGVLPSDHPVTAVLRNPQGQIIRSIVNNNGFNGMYAFRFQTVDSDPT
                     GHWQVDVTAGGQTYSKSVRIETVKPNKLLIDLQMDEKEVVPVDLVNGNLSVKWLVGSA
                     AADLEARIDIECFRGVTEFAKFKNFVFEDRSRSFSQATYNLTPGRTDQDGRLSFNYRL
                     GGIGEQPGMLRAVFTTRVFEKSGDFSIDSYTTTISPYKTYLGINVPEAEDYWGDPYLD
                     KSKTHTIKLAAVDYKGNPASGRVDAEVEVYKMGWSWWWGSSSSELASYSKDSYNKPYK
                     TFKVSIVNGESSFPLTFTDESGYYFIRVRDPQGGHATSYVTQVYTGDDKRADAGSESA
                     TRLATSLDKDKYKVGEEAQLTIPSSPGARVLVSIEKGESVLKTFWMTCADTKTTIRIP
                     IESGMTPNVYASVTLIQPHNTTVNDAPIRLFGIQRIMVEDDATHLNPKIAIADEVRPE
                     SKVTLTVSESDGRPMSYVIALVDEGLLSLTRFKTPNPWSAFYATEALGVRSWDLYDLV
                     IGAYGARMEQMFAIGGDGEVEEIAAANKAERFKPVSLFLGPFTIKSRSKQNHEITLPA
                     YIGNLRVMVVATDGKAQGAAEKNVAVTKPLMVQSTLPRVIGTDEEVVIPITLFAMKHN
                     LGKVTVSLSTNDLLDVSGSSSQTLNFTAAGEQMAFFKVKASSAEGIAKITAKAKCSTD
                     NAEEKIEIDIRDPNPLTTNAKTILLAGGKSQKVTFAFAGKQGTNSAFVEASSIPPVDL
                     SYRMDYLVKYPHGCIEQIVSSAFPQLYLGAVQTLSDEQKSECEKNIKATITKIPSYAL
                     NNGGMTYWPNTSSYASASTWGTIYALHFLVEAEAKGYAVPAWLKRSMTSFVKDVASST
                     SHAYPYRTYAAYVLTLTGAAPRGDMNRMRNEVDNMNTESGWFLALAYAADKKSDVAKN
                     IISKVSNRPVGKIDRFSYSFESEDRFKAVSAIAYRLTGNKQEAFKSIETLSKSLNDRN
                     HFMSTQSTAWALNAVAAYAAQYSEEGVNVTAKAGRESITLKDDKCIVRRTLDVTKGES
                     IDFEFTNKTDAPTYIVMSSTGVPAKGQETDFSNGLRLSVSYTLPNGTPVDPFSLTQGT
                     DFVIKARVTNTSATENYTNLILSQIFPSGWEIANDRTTDFYQDYRDDRVYSYFNLGKS
                     SSKEITIRATATYKGKFYLPSTICKAMYDETVNASVKGGWCEVK"
BASE COUNT         1653 a         1424 c         1301 g         1731 t
ORIGIN      
        1 taagcctgcc gctagcgttc atcctgagcc aggatcaaac tcttcattgt ataaaaaagt
       61 tatgtctatt gtctgaacct gatactcatt attgaagtga ttattcaaaa aaattaacgt
      121 ttcgctcgaa tttttgttac aaaattcggt acttgcttgt actttttgtc ttccaatttt
      181 atcaaggaac ggacgtctct tttaagaaac gggttgcaaa gatagagaca tttttttaat
      241 tgggcaaaaa aatgctcaaa aaaagattat tcttttattt cttcataatc aacatcctcc
      301 acaagttctt tggcaggatt tgcatagctc tctgacaatt tttcttttat tgccttgcga
      361 atcttgatag tcgcgtagag ttcaaacaat ccataaccca gaaaacctgc acccagaagt
      421 tggaacatca ggatctgaga ctcgtgagga ttgaacacca agaagaggcc acaaagagcc
      481 acaatggtag gcagcacata catataccag ggtattggag cgtatttatg cgccgcaata
      541 aatgtgacga tctcaccgat accgaaaacc accaaaatga ttccaagcag tagcaataag
      601 aaacctacga acacatcagg gataagaatc aaaacagcac cgaaggcgat actcagtata
      661 gcattcacta caaagaaatt cttctttacc ctatcggtag tagcttgtga ttttatactc
      721 tttctcctgc tgataaaata gataacgagg gaaacacagc cgatcaccag aaatagtcca
      781 ccgatgatgc gcactattag atccttcatc gtatcaggcc agattagcag cacgagaccc
      841 gctacaatgc caaccaaagc tcttaagtaa ccataagtgg gattaaaaaa acttttgaat
      901 gacttcatat gattatatct gacattactc ttattatttc acttcgcacc aaccaccctt
      961 tactgatgca ttgactgtct catcatacat cgccttacaa atagttgaag ggagatagaa
     1021 cttgcctttg taggtagcgg ttgccctaat ggttatctcc tttgacgacg atttacccag
     1081 gttaaagtag gaatataccc tgtcgtcacg gtagtcctga tagaaatcag ttgtacgatc
     1141 atttgcaatt tcccatccgg aagggaatat ctgtgatagt atgagattgg tataattctc
     1201 tgttgcgctg gtgttggtca cacgtgcctt gattacaaag tccgttcctt gcgtaagcga
     1261 gaatggatct acaggtgtgc cattgggcaa tgtgtagcta accgagagac ggagtccgtt
     1321 ggagaagtct gtttcctgac cctttgccgg tacaccggta gagctcataa caatataggt
     1381 aggagcatct gtcttgttgg taaactcaaa gtctatgctt tcgccttttg ttacatccaa
     1441 tgtacggcga actatacact tgtcgtcttt gagagtaata ctctctcttc ctgccttagc
     1501 agtaacgtta actccttcct cagagtattg tgcagcatat gcggccacgg cattcaaagc
     1561 ccaggcggtg ctttgcgtac tcataaagtg attgcggtca tttagcgatt tggatagagt
     1621 ctctatcgac ttgaatgcct cctgcttatt gcccgtcaga cggtatgcaa tggctgaaac
     1681 ggccttgaat ctatcttcac tctcaaatga ataggaaaat ctgtcaattt tgcctacagg
     1741 acggttggat acctttgaga tgatattctt ggcaacgtca ctcttcttat cggcagcgta
     1801 tgccagggca aggaaccagc cggactccgt attcatgttg tcaacttcgt tgcgcatgcg
     1861 gttcatatca cctcggggtg cagcaccggt taacgtaagc acataggcag cgtatgtgcg
     1921 gtaaggatat gcatgagatg ttgaagaggc cacatctttg acaaatgagg tcatactgcg
     1981 tttgagccag gcaggtactg cataaccctt agcctcagct tcaaccagga agtgaagcgc
     2041 atatatggta ccccatgtgc ttgcagaagc atacgaagag gtattcggcc agtaggtcat
     2101 acctccatta ttgagagcat aagagggaat tttcgtaata gtcgccttga tattcttctc
     2161 gcattcgctc ttctgctcat cactcagtgt ctggactgct ccaagataga gctgaggaaa
     2221 ggctgaagag acgatctgct caatgcatcc atgggggtat ttcacaaggt aatccatgcg
     2281 ataacttaga tctaccggtg gtattgaact tgcctcaacg aaggcggaat tggtgccctg
     2341 tttgcctgcg aatgcaaaag tcaccttttg gctcttgcca cctgccagca atatagtctt
     2401 ggcattggta gtaaggggat tgggatccct aatgtcaatt tctatcttct cctccgcatt
     2461 atctgtagaa catttagcct ttgcagtgat ttttgcaatg ccttcggcag atgaggcctt
     2521 tactttgaag aatgccatct gctcaccggc agcagtgaaa ttgagtgtct gtgatgatga
     2581 tccggataca tctagtaaat cgttggttga aagtgatacg gtaactttac ccagattatg
     2641 cttcatcgca aagagagtga tgggaatcac aacctcttca tcggtgccaa taactctggg
     2701 tagagtactt tgcaccatta gcggcttagt gactgcaaca tttttctctg ccgccccctg
     2761 tgctttacca tcagtggcca ctaccatcac gcgaagatta ccgatatatg caggaagcgt
     2821 gatttcatga ttttgtttac ttctggattt gatagtaaaa gggcctaaga agagagatac
     2881 cggtttgaat ctctcagcct tattggcagc agcaatctct tcaacttctc catcacctcc
     2941 aatagcaaac atctgctcca tcctcgctcc gtatgcacct atcacaagat cataaaggtc
     3001 ccaactacga actccgaggg cttcggtagc atagaaagca ctccatggat taggcgtctt
     3061 gaatctcgta aggctaagca atccttcgtc taccaaagct atgacgtagc tcataggacg
     3121 gccgtcactc tcactgacag tcagtgtaac tttactttcc gggcgaacct cgtcagctat
     3181 agctatctta ggattgaggt gagtggcatc atcctctacc attatccttt ggattccaaa
     3241 caaacgtatg ggagcatcat tgaccgtagt gttgtgaggc tgaatcaggg ttactgacgc
     3301 atagacattt ggagtcatcc cgctctcgat agggatacgg attgtggtct tggtatcagc
     3361 acacgtcatc cagaaggttt tgagtacgct ttcacccttc tctatggaga caagcactct
     3421 ggcacccggt gaagaaggaa tggtgagctg tgcctcctca cccactttgt atttatcctt
     3481 atcaagggaa gtagcaaggc gggtagccga ttcgctgccg gcatcggcac gcttgtcatc
     3541 tccggtataa acctgtgtaa cgtatgaagt ggcgtgaccg ccctgagggt ctcttactct
     3601 aatgaaatag taccccgatt catcggtaaa tgttaacgga aatgaactct caccattcac
     3661 aatggaaacc ttaaaggttt tgtaaggctt gttataagaa tccttagaat aggatgcaag
     3721 ctcactgctg gaagaacccc accaccaact ccatcccatc ttatagacct caacctctgc
     3781 gtcaacacga ccactcgcgg gattaccttt gtagtctact gcagcaagtt ttatggtatg
     3841 tgtcttactc ttgtcgagat aagggtcacc ccagtaatcc tccgcttccg gaacattaat
     3901 acccaaatag gtcttgtagg gtgaaatcgt agtagtgtat gagtcgatac tgaaatctcc
     3961 gctcttctca aagactctcg tagtgaagac tgctctgagc atacccggtt gctcaccgat
     4021 gccgcccaag cgataattga aagagagacg tccatcctgg tctgtccttc ccggagtgag
     4081 attgtatgtg gcttgactaa aacttctcga acgatcttca aatacgaaat ttttaaattt
     4141 tgcaaactcc gtaactcccc ggaaacactc gatatcgatt cttgcctcaa gatctgcggc
     4201 cgcagagccc acaagccatt tgacggaaag attaccatta actaaatcta cggggactac
     4261 ctctttctcg tccatctgga gatctatcag gagtttgttt ggcttgaccg tctctatcct
     4321 gacgctcttt gagtaggttt gaccacctgc agtcacatcc acctgccagt gacctgtggg
     4381 gtcactatcc acagtctgga atctgaatgc atacattccg ttaaagccgt tgttattgac
     4441 aatcgagcgt ataatctgtc cttgaggatt tctcaaaact gctgtaacgg gatggtctga
     4501 gggaagtacc ccctcctcca acatagaaat aaaagtgata tagatgtcgt ctcccggtct
     4561 ccaaactcct ctttccccaa aaatataacc cttctggcct cctgatacgg atgtacctcc
     4621 aacatcaaag ttactaacag agagtgatgc tccatagttg agtttgagat aattcttgtc
     4681 actgccgctg gtaactactg ccgtatgggg ttcttcggac agttttatct ccactttgcc
     4741 gtcactgcct gtagaaccct caccaattac ctgattaaca taatcgaaga ctctaacttt
     4801 agctcccgac acaggggatg cgtcaatgat atttgtcaca aagaaggtat aggtgccttt
     4861 cccggaaccc ttggctatta cagcaaggtc agaagcgaga atgttgacat gcctctcttc
     4921 gtaatcgtag taggagccaa aatagtaatc gctatcataa taatccgagt cctgctccac
     4981 aagagggtca acgcccttaa tgccgatacg gtaaagtgca cctttctgaa cctttataag
     5041 gtcggttata ttgagaccgt aactggccac acttttaagc ttggatgatg aagggtcagc
     5101 caaaaccaaa accgtgtcca ctacaacctt ggctacgcga cccagtatat aattgctctt
     5161 ttcatcgagt ctttcagact gaagatactg gagcatatta ttttcgtaaa tgcgatgtac
     5221 cctcacctgt acttttgcat aattctgaga ttggaagaag agtttgttgc cattggatga
     5281 gggcagcacg actcccttgg tagagaattt tataaaaggt tttttctggg gaacatcaaa
     5341 agtttgctcc caatcaaaac cgagctgctc acctttggca ttcttaacaa gtttactgat
     5401 attaaccgtc tgcctcagca catcctcgat tgaaggatat atggtaagct tattcctgtc
     5461 aacaagaaac tttagtttac ctgcatttgg aagttttaca aggttagcca taacctgttt
     5521 tttatcaagg gccgaagaga atactatctc atagcggtaa gggtcgagca agggttcagc
     5581 gctgattata acaaatgctc cctcagcggg aatattgaat gaccaggaac ccatatcatt
     5641 aaacaactca tactttcggc ttatggtaaa aacggactgc tttttctccg gaattatgtt
     5701 gttgattgag agccggtgta caaggccatc ctccgcatgc tgccaggaaa cggggaagtc
     5761 tttgctcagg ctgatacctt tctctatata ctcgttgcca accggatctg aggtggttat
     5821 gacaatatcg agatggtatt tatcctcctc atcgggataa acggtaattg cacccaattc
     5881 atagtgaatt tcaggagcca atgtcttaaa attgtaagtc accttagcgg atcttctgcc
     5941 tacaagctta ccagcatcta tctgaacttt atactcggca ttgaagtcca acggctcggc
     6001 aaagaagaga gccacagtac gctcatcaat cagtgatacg ctgaactccc tttttggagt
     6061 aatctggatc aactttttca gttcatcggg cgtattgtcg cctacattg
//
LOCUS       DAOSIE010000067         6011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_11908_length_6011_cov_3.701310, whole genome shotgun sequence.
ACCESSION   DAOSIE010000067 DAOSIE010000000
VERSION     DAOSIE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11908_length_6011_cov_3.701310"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLI69_03165"
     CDS             complement(<1..686)
                     /locus_tag="PLI69_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG41521.1"
                     /translation="MKHNSDIFDATLCDFRSCLKLERSLSDNTIAGYNSDCAKFFAFI
                     KGKGIISPEAVSYEDISEYLSHATSAGLSKRSQSRLVSSLKSLYRFLESEGRLKDNPC
                     DKIDTPKAGRYLPEVLSVDEVERIINSVDLSKSEGHRNKCIIEVLYSCGLRVSELVGL
                     RISDLFLAEQFIRVTGKGSKQRLVPIGEPAVQSISLYMEIRNLGPISKGAEDILFLNR
                     SGGKLTREMI"
     gene            784..1281
                     /locus_tag="PLI69_03170"
     CDS             784..1281
                     /locus_tag="PLI69_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41522.1"
                     /translation="MKKIIILLAVALIGVLPLSAQSTSDQATESGQKTHKVSTTFGAV
                     GAHIGYAFWGTTVGLHGDFNANNFRGRAGFDLFFSEHLNLGADVGFHYLLHLIDGLNV
                     YPIIGVNMGFGKNVDFHVGMDFGAGIEYNFANGWSIFLDGKYNLKLLGPDIPYRSYIG
                     VTKRF"
     gene            complement(1288..1923)
                     /gene="nth"
                     /locus_tag="PLI69_03175"
     CDS             complement(1288..1923)
                     /gene="nth"
                     /locus_tag="PLI69_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOG41523.1"
                     /translation="MMNAKKYQQVITWFEANMPVAESELDYKNPYQLLCAVILSAQAT
                     DKRVNIITPALFERYPTPEDMAASNENEIYQIIRSVTYPNSKARHLLGMARMLVSDFG
                     SVVPDDVDELQKLPGVGRKTANVIASIVYNKPVIAVDTHVFRTARRLGLSSGNNVLEV
                     EKDLTAGFPEHLRAKAHHWLILHGRYVCTAKKQKCESCGLAPYCDFFNQNK"
     gene            complement(1880..2578)
                     /locus_tag="PLI69_03180"
     CDS             complement(1880..2578)
                     /locus_tag="PLI69_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG41524.1"
                     /translation="MDENDIIIEFEDVEITNGEIPVITSLNLTIREGEFIYLTGKVGS
                     GKTSIIRTITAENPINTGNARVGDFNLSNLKRRKIPYLRRKIGVVFQDFQLLMDRSVE
                     DNLLFVLKSTGWKDRKLMEERINSVLEAVGVASKAHKMPHQLSGGEQQRVAIARALLN
                     EPRLILADEPTGNLDGETAAGIMRLLFEINRTRKTAILMVTHNMNIVREYPGRVLVCA
                     DNECKEISTGNNLV"
     gene            2857..3366
                     /locus_tag="PLI69_03185"
     CDS             2857..3366
                     /locus_tag="PLI69_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM-associated putative lipoprotein"
                     /protein_id="HOG41525.1"
                     /translation="MKKVALKTYGKLLTLFFSLVGMITGCDWFEPSYEYGCPSADYII
                     KGKVTDKQSKKPIKNIAVIRKAYSSPYGNDTVRTDFRGDYELKYRHYGGEDLIIVEDL
                     DKERHGGWYAPDSIKVDWKQMQKIKKGDGRWYDGVFLRADTNFELEHQTIALYGVMSA
                     EYKEMKEEE"
     gene            3384..4457
                     /locus_tag="PLI69_03190"
     CDS             3384..4457
                     /locus_tag="PLI69_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HOG41526.1"
                     /translation="MKLRSKILLNLYREHRKTQAKLHELTYFFWECTLRCNFNCIHCG
                     SDCTRESVTPDMPKEDFLKVLADIRPHLNPNKTMIVLTGGEPLMRSDLEECGRAMYDM
                     GYPWGFVTNGWGLSQERLNNLLRAGLRSVTVSLDGYTAESHEWFRGKKGSWSRAVDAI
                     RRVASTPDLVYDVVTCVNKKSLPDLHKLKELLISLGVTHWRLFTVFPKGRAKDNPLLR
                     LSVDEFVQMMDFIKQTRKEGIIRASYGCEGYLADYEMQVRDAPFFCQAGIHIGSVLAN
                     GDISACPSLRGDYIQGNIYKDDFWTVWNTRYQIMRDRSWTKMGKCATCKSYKYCEGNG
                     LHLRDEQTGELLQCHLEMMGKLN"
     gene            4670..>6011
                     /locus_tag="PLI69_03195"
     CDS             4670..>6011
                     /locus_tag="PLI69_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41527.1"
                     /translation="MKIKSFTYFLVFLLLMLSAGAMAQEHRVSRIESALDLYRKGAFY
                     SAEQLLNLVTPDPDSDISIFAIQVEAYKTMCAIRLDRSNIAGLVKNFEDRYPNAPELQ
                     MVKFALASRLFDREDYAAALAIYDSIKKPFLYAEEQIEYDFKSAYCYIREGNTDVAAE
                     RLTSIIEGDYSPFSVPSQYYLGYVRYLEKDFAAALPHFRKAEKDPRFSVLASYYTVES
                     EFMLANYRYVIDRGPAIFSTLERDLQINLARIVSEAYFSMGQPTLAAQYFDMFSTSGG
                     NLTRKDYYYSGLLSYTLQSFSKALDSFSNVVGNEDALGQSAYYYSANSHLRLHNKIAA
                     MEAFKAASESDFDSVIKEDALFNHAKLSFDVNGDISGFEKYMENYPESGKDDIINSYI
                     AAAYLLSNDYNSAVLALTKIRELSDESSSNLQKAAFFRAMQLIENKGYRPAVPML"
BASE COUNT         1615 a         1239 c         1384 g         1773 t
ORIGIN      
        1 aaaatcattt cacgggtgag ctttccaccg ctacggttaa gaaagaggat gtcttccgct
       61 ccttttgata taggtcctaa gttgcgtatc tccatataaa gggagatgct ctgtactgca
      121 ggctccccaa tgggtaccag gcgctgcttg gatcctttac cggttacccg gataaactgc
      181 tctgccaaaa agagatcgct gatgcgcaga cctaccagct cactaactct caatccgcag
      241 gaatagagta cctcaatgat gcacttgttg cgatgcccct cgctcttgga aaggtcgact
      301 gagttgatga tgcgctcaac ctcatctacg gaaagtactt ccggaaggta cctgcctgct
      361 ttcggagtat caattttgtc gcagggatta tccttgagtc taccttcact ctctaaaaag
      421 cggtaaagtg actttaaaga actgactaaa cgggactggc tccttttgga gagaccggcg
      481 cttgtagcat gcgaaagata ttccgagata tcttcatagg ataccgcttc aggggaaatg
      541 ataccctttc cttttataaa tgcaaagaac tttgcacaat cggagttgta tcccgctatg
      601 gtgttgtcag acaatgacct ttccagtttt aggcaagacc tgaaatcgca aagtgtcgca
      661 tcgaatatat cagaattgtg cttcaaaaaa tgatgagaat tttaaaaaag tgttacattt
      721 gtccactgta tcaaagataa agatacagca aatttagaat tttaactaac aaaattttat
      781 caaatgaaga aaataataat cttgctggca gttgcactta tcggcgtttt acccttatca
      841 gctcagagca cttctgacca ggcaaccgaa tcgggtcaga agactcataa agtctccact
      901 acttttggtg cagtgggtgc acacataggt tatgcattct gggggacgac ggttggtctc
      961 cacggtgatt ttaatgcaaa taattttagg ggaagagcag gtttcgacct gtttttcagc
     1021 gaacatctta atctcggtgc cgatgtgggt ttccactatc tgcttcacct cattgacggt
     1081 ctcaatgttt atcctatcat aggtgtgaac atgggatttg gcaagaatgt cgactttcac
     1141 gtaggcatgg acttcggtgc aggcatcgag tacaactttg ctaatggatg gagcatcttt
     1201 ttagatggta aatacaacct taaacttctt ggtcccgata ttccttacag atcttatata
     1261 ggtgttacca agagatttta atcagcatta tttgttttga ttgaaaaagt cacagtaagg
     1321 tgcgagtccg caggattcgc acttttgttt tttggcggtg catacatagc gaccgtggag
     1381 tatcagccag tgatgcgctt ttgcacgaag gtgttccgga aaacctgcgg tgaggtcttt
     1441 ttcaacttca agcacattgt tgcctgatga aagtcccaga cgtcgtgcag tacggaaaac
     1501 atgcgtatcc acggctataa ccggcttatt gtagacaata gatgctatga cattggccgt
     1561 cttacgtccg acacctggta gtttctgtaa ctcatccaca tcatctggca ccacggagcc
     1621 gaaatcactc accagcatac gtgccatccc gagcaggtgg cgtgccttgc tgttggggta
     1681 tgtgacggag cgtataattt gatagatctc attctcatta gaagcagcca tatcttcggg
     1741 tgttggatat cgctcaaaga gggccggggt tattatattg acacgtttgt ctgtagcctg
     1801 ggctgaaagt atcacggcac aaagcagttg atatgggttc ttataatcca gttcactttc
     1861 ggctaccggc atattagcct caaaccaagt tattacctgt tgatatttct ttgcattcat
     1921 tatctgcgca tacaagtact cttccgggat attcccgtac tatattcata ttgtgtgtaa
     1981 ccatcaggat agcagtcttg cgtgtacggt tgatctcgaa aagcaggcgc attatgcccg
     2041 cagcggtttc gccgtcaaga ttacccgtgg gctcgtctgc aagtatgaga cgaggctcgt
     2101 tgagtagagc gcgggctatg gctactctct gctgctcgcc tcctgagagc tgatgcggca
     2161 ttttgtgggc cttgctggcc actcctactg cctcaagcac gctatttatt ctctcttcca
     2221 taagcttgcg gtctttccat ccggtggatt taaggacaaa aaggagattg tcctccacgc
     2281 ttctgtccat caggagttgg aagtcctgga aaaccacacc tattttgcgt ctgagatagg
     2341 gtattttcct tcttttaagg ttggagaggt tgaaatcccc aacacgggca ttgcctgtgt
     2401 taatgggatt ttctgcggtt attgtgcgta tgatggaggt tttaccactg cctactttgc
     2461 cggtgagata gatgaactct ccttcacgta tagttaggtt gagggaggtt atgacgggaa
     2521 tttctccgtt tgtgatttca acatcctcaa attctatgat aatatcgttt tcgtccatgt
     2581 ttatgacact gtttggatgc aaaattacat ttttattccc attaaataaa tctcagttga
     2641 caattttggt ttaaatcagc agttttaact taattttgtt gataatagcg gaaaatgcca
     2701 ctctaattgc aacgattttt catttttatg catctttata atataagata ttttgtcgta
     2761 cactctttaa tcaatgatgg aagtatttcg tgagtcactg cgtatataaa gtaattggac
     2821 atttttttat ttaaatatct cacggcaaag aaagtcatga aaaaagtagc attaaaaaca
     2881 tacgggaaat tgttaaccct ctttttctca ctggtaggaa tgataaccgg gtgtgactgg
     2941 ttcgaacctt cttatgaata cggatgtcct tctgcagatt atattatcaa ggggaaagtg
     3001 acggacaaac aatcgaaaaa gcctattaaa aatatagcag tcatcagaaa agcctactct
     3061 tcgccctatg ggaacgacac ggtaaggaca gattttcgtg gcgactatga gttaaaatat
     3121 cgtcattacg gaggtgaaga tcttattatt gtcgaagatt tagacaaaga gaggcatggc
     3181 ggatggtatg ctcccgattc tatcaaagta gattggaaac agatgcaaaa gattaaaaaa
     3241 ggtgatggaa ggtggtatga cggagtcttt ttaagggctg ataccaactt tgaactggaa
     3301 catcagacta tcgctctgta cggtgttatg tcggcagagt ataaggaaat gaaagaagaa
     3361 gagtagcaaa aagcagatgt aaaatgaagt taaggagcaa aatcctactg aatctatacc
     3421 gtgaacacag aaaaacgcaa gcaaaacttc acgaacttac ctatttcttt tgggaatgca
     3481 ccttgcgttg caatttcaat tgtatccatt gcggcagtga ttgtacaaga gagtccgtta
     3541 ctcccgatat gccaaaagag gattttctga aagtgcttgc ggatattcgt ccgcatctga
     3601 atcccaataa gacaatgatt gtgcttaccg gaggcgaacc gttgatgcga agtgatttgg
     3661 aggagtgcgg gagggccatg tatgacatgg gatacccctg gggatttgtt accaacggat
     3721 ggggactttc acaagaaagg ttaaataacc tgctacgcgc cggattgcgt tcggtcaccg
     3781 taagcctgga tggatatacg gctgagtccc acgagtggtt tcgcggtaaa aaaggctcct
     3841 ggagccgtgc tgtagatgca ataagacggg tggcttcaac tcccgatttg gtttatgacg
     3901 tggttacctg cgtcaataaa aaatcattgc cggacctgca taagctcaag gagctgctta
     3961 tttcgcttgg cgttacccat tggcgactgt ttacggtttt tccgaaagga agagcaaaag
     4021 ataatccgct gctcaggctt tccgtagatg agttcgtgca gatgatggat ttcattaaac
     4081 agaccaggaa agaggggata attcgtgctt catacggctg tgaaggctat ttggcagatt
     4141 atgaaatgca agtgcgcgac gcacctttct tctgccaggc cggtattcac atcggttcgg
     4201 tcctggcaaa cggagatata agcgcatgcc ccagcctgcg tggcgattat attcagggaa
     4261 atatctacaa agatgacttc tggacggtgt ggaatacacg atatcagata atgcgcgacc
     4321 gttcctggac caaaatgggg aaatgtgcca cttgtaaatc ttacaaatat tgcgaaggaa
     4381 acggcctgca tctccgcgac gagcaaaccg gcgaactgtt gcaatgccat ttggaaatga
     4441 tggggaaatt gaattagttt cgagtttcga gtttcgggtt tcgggtttcg agtatggagc
     4501 cggttccttg ttctgagtat gaatcattct taactcagat tttttttgta cttttgtagt
     4561 ttatgttttg tgagttgttg ggctctttta ttaccatata aataaaagct tcacctctca
     4621 tttaacttta tatgagattt tggacctttt tttgagggat agatattgga tgaaaattaa
     4681 aagcttcaca tattttcttg tgtttctact cttgatgttg tcagcaggag ccatggcaca
     4741 ggagcatcgc gttagccgta tcgagtcggc gcttgatctg tacagaaagg gggccttcta
     4801 ttcagccgag cagttgctaa accttgttac acccgatccc gatagcgata tctccatttt
     4861 cgcaattcag gtagaggcat ataaaacaat gtgtgccatc cgtcttgaca ggtccaacat
     4921 tgcaggtctg gttaagaatt ttgaggatag atatcctaat gctcccgagc ttcagatggt
     4981 taaatttgca cttgcttcgc gtctttttga cagggaagat tatgcggctg cccttgccat
     5041 ctatgattcc ataaaaaagc ccttcctcta tgctgaggag cagattgaat acgacttcaa
     5101 aagtgcttat tgttatatca gggaaggcaa tactgatgtg gcagccgaga gactgacctc
     5161 tatcatcgag ggtgattaca gtcctttctc tgtgcccagt caatattatc tgggttatgt
     5221 gcgttatctg gaaaaggatt ttgctgctgc cctgccgcat ttccgtaaag cggaaaaaga
     5281 tcctcgtttc tcggtattag cctcatatta tacggtagaa tcggaattta tgcttgccaa
     5341 ttaccggtat gtgattgacc gtggccccgc cattttcagt acccttgaac gcgatttgca
     5401 gataaatctt gctagaatcg tctctgaagc atatttttct atggggcaac ctacattggc
     5461 agctcagtat ttcgatatgt tcagcacttc cggaggtaat cttacccgta aggactatta
     5521 ctactccgga ctactttcat ataccctcca gtcatttagt aaggccttag actctttcag
     5581 caatgttgta ggcaatgaag atgcactcgg ccaaagcgct tattattaca gtgccaacag
     5641 ccatctccgc cttcacaata aaattgctgc aatggaggca ttcaaagctg cctctgaatc
     5701 cgattttgat agcgttatca aagaagatgc tcttttcaat cacgctaaac tctctttcga
     5761 tgtcaatggc gacatatctg gatttgaaaa atatatggag aattatccgg agagtggtaa
     5821 agatgatatc atcaatagtt atattgcggc agcatacctc ctgagcaacg attacaattc
     5881 tgcggtactc gctcttacaa agatccgtga actttcggac gaatcttctt cgaatttgca
     5941 aaaagcggca tttttcagag cgatgcaact catcgaaaat aagggatata ggccggctgt
     6001 tccgatgctg g
//
LOCUS       DAOSIE010000068         5978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_11970_length_5978_cov_2.713321, whole genome shotgun sequence.
ACCESSION   DAOSIE010000068 DAOSIE010000000
VERSION     DAOSIE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11970_length_5978_cov_2.713321"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1912)
                     /locus_tag="PLI69_03200"
     CDS             complement(<1..1912)
                     /locus_tag="PLI69_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41528.1"
                     /translation="MKNIFGKLKKLLPYLVAAAIFILLAVVYCYPVLSGKVLRQGDVY
                     QWKGMAQEVYEYYNKTGNVSNWSGSMFSGMPAYQISNFPPQSGFEGFMFKFLYFFHNI
                     VHLFFNSVIGIIIGYFVGFYILIRSFNIDKWLSIVGSIAITLSSYFFIIIVAGHETKA
                     ITLGLMPPVIAGFYLIFRKKYPWGVCLTMLFTALGVYRHPQMSYYIFMMIGVLAIGEI
                     WQHIKEKRFKDMAIAFFLFVLSVFIGAGTGYGNYKSNAEYVKETMRGGHSELVKEGAV
                     TSSNESGLSFDYATQWSYGIGETFTLMIPNFKGGASSSDIGTESLFAEALKSKGVDPQ
                     TVKYYSSVAPTYWGEQPFTAGPVYVGAIVCLLFVFGLCIVKGPYKWGLLIATLFSILL
                     SWGHNFEWLSKLFFNYFPLYSKFRAVSSILVVAQIAMPLLGFLALREIFEKRVDFRQL
                     KRALLVALGITGGLSLFFALFGGLIFNFTSSGDAYMAAQFPDWFMEALIDQRAAMFRS
                     DSFRSLVFILLAALLILLFAKQKIKPALFTLLLGVLIVADMWPINRRYFNDSHFVTSK
                     DNDSYFQKYAYEEEILKDPDPHFRVLNLTTNTFNESRTSYYLKSIGGYHAAKLRRYQD
                     LIDEHLTQMNMKV"
     gene            complement(1924..2856)
                     /locus_tag="PLI69_03205"
     CDS             complement(1924..2856)
                     /locus_tag="PLI69_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOG41529.1"
                     /translation="MADFWKVIVSSMSGGGKAGREWPEIAARLEARGIAYSVSFTEYR
                     FHAREIAAEALKKGIRQMIVVGGDGALHEVLSGIMLQNKVPSNQVTLAIIPVGSGNDW
                     ARLHEIPRGYDDVTDVIARGKSVLQDVAKVESFMDGAPFISYMVNIGGLGFDAQVCHN
                     FGLEKKEGQATSDALYLKCLLKGFMGYDARKFSIVADGNLFYEGDVFSVAMGIGRYSG
                     GGMIQTPNAKFDDGLIDLTVIKDIPKSKILRKIPTLFTGKIHDVNEVIHTQARVLEIS
                     AEPESFVEVDGEEVGLSPIRVEVIPAAVRVITNL"
     gene            2959..3747
                     /locus_tag="PLI69_03210"
     CDS             2959..3747
                     /locus_tag="PLI69_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG41530.1"
                     /translation="MGKIIAIANQKGGVGKTTTAINLAASLAVLERKTLLIDADPQAN
                     TTSGLNFNPDADSENTLYEVLIGQKDIEQTIMPTELDYLDIITSHINLVGAEIEMLNV
                     ENRESVLKKALAVLRDRYEYIILDCAPSLGLITVNALTAADSVIIPVQPEFFALEGLG
                     KLLNTIKLVQNRLNPALNIEGFLFTMFDSRLRLHNQVVEEVKTHFKEMVFSTIIVRNI
                     RLSEAPSFGKPAILYDVGSPGASNYLELAKEIIKKNQSWQKEKH"
     gene            3726..4634
                     /locus_tag="PLI69_03215"
     CDS             3726..4634
                     /locus_tag="PLI69_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOG41531.1"
                     /translation="MAKREALGRGLGALLGDAGEIRQPEYRPVSPSERSNPASICEVP
                     LSQIIPNPAQPRLSFDEEGLLELADSIRTLGIIQPLTLRKIGPSQYQIISGERRFRAA
                     KLAHLQTVPAYIREADDTSMLEMAIVENIQREDLDAIETALGFQRLMEECNLTQEEMS
                     SRIGKKRATVANYLRLLKLPVEIQKAVKTGKISVGHAKALLSLDNHAQQIELCELTIR
                     KDLNVRQLEEKVRQSSIPAPVQQSNNRQDNYPESYYRLADIVGKYFDRGVSFKRTDNG
                     GGTMTVRFKNDNELDAFLKVIEDKQL"
     gene            4643..5593
                     /locus_tag="PLI69_03220"
     CDS             4643..5593
                     /locus_tag="PLI69_03220"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOG41532.1"
                     /translation="MKRSLAVIFLLIVFHFGAEAQFQRSGNVTMGQMGGPPGANMQRD
                     TQVNPESDVKADTTAGFSMKALIRGYMGRDTLKPGYMLLGTAVLPGAGQIYNKQWWKV
                     PIIYGGIGAGIYTGVSNNLKYQETGDQKYKLYRNLGYISAGLTYWGSLLDATISYKTE
                     VPVPVAAKSTIYSALLPGLGQAYNGDYWKIPIWWSGFVACGYFFHQNDIQYKRFKYIC
                     KMDNDPNSGYIGNITSGQAEWYRDTYRRFRDYSVVAFVLVYALNIIDANVFAHMADFD
                     VSDNLATLQFEPAIIQPLTPYYSPTYAPMHSFGLQMTLNF"
     gene            5598..>5978
                     /locus_tag="PLI69_03225"
     CDS             5598..>5978
                     /locus_tag="PLI69_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41533.1"
                     /translation="MKARTLISIVILLTSLLSFLPDAGAQIRSRKQIIEDLTRRLDSL
                     QNAYDSLAFEYNLLAEPVSEDVEVEATMEKEFKTETYTPESIDSLRSVWYLHKKAEAF
                     NPDFEMLERDTLRSSIPDSVYIERL"
BASE COUNT         1779 a         1415 c         1262 g         1522 t
ORIGIN      
        1 tcaccttcat attcatctgc gtgagatgct cgtctataag atcttgatac ctgcgtagtt
       61 tagctgcatg atagcctcct atggacttga gatagtatga agtgcgcgac tcgttgaaag
      121 tgttggtagt aaggttcagt accctgaagt gaggatcggg atccttgaga atctcctctt
      181 cgtatgcata tttttggaaa taagagtcgt tatccttgct agtcacaaaa tggctgtcat
      241 taaaatatct tctgttaatc ggccacatat ctgcaacaat aagtactcca agcagtaggg
      301 taaagagtgc cggtttaatt ttttgctttg caaaaagcag aatcagaaga gctgcaagaa
      361 gaataaacac gagagaccta aaactgtcac tgcggaacat cgcagctcgt tggtcaatga
      421 gtgcctccat aaaccagtcc ggaaattgag ccgccatata ggcatctcct gaagaggtaa
      481 agttgaagat aagtccgcca aaaagtgcga agaacagaga aaggccacct gttatgccaa
      541 gcgcgaccaa aagtgctctt ttgagttgcc ggaaatcaac tcgcttctca aaaatctctc
      601 ttaatgcaag aaagcctagc aatggcatcg ctatctgagc caccacaagt atcgaagata
      661 cagctctgaa tttactatag agagggaaat aattaaaaaa gagtttggat aaccactcaa
      721 aattatgtcc ccatgagaga agtatcgaaa atagggttgc tatcaataat ccccacttgt
      781 agggtccctt tactatacag agtccgaaca caaagagcag gcagactata gctcctacgt
      841 ataccggtcc cgcagtgaag ggctgttccc cccagtatgt gggtgcaacg gaggaatagt
      901 attttaccgt ctgaggatcc actcccttgg atttgagggc ctccgcaaaa agtgattcag
      961 taccgatatc agaacttgag gcacctcctt tgaaattggg tatcataagg gtgaaggtct
     1021 cacctatacc gtagctccac tgagtggcat agtcgaaact cagtcctgac tcgttgcttg
     1081 aagtgacagc cccctctttc acaagttccg agtgtcctcc ccgcatcgtt tccttcacat
     1141 attcggcatt ggatttatag ttgccgtagc ccgtgccggc accgatgaat accgatagta
     1201 caaaaagaaa gaaggcaatt gccatatcct tgaagcgctt ctctttaata tgctgccaaa
     1261 tttctccaat cgcaagaaca ccgatcatca tgaagatata gtatgacatc tggggatgcc
     1321 tgtatacccc caatgcagta aataacattg tcaggcaaac tccccaaggg tatttcttgc
     1381 gaaatatcag ataaaatccc gctatcacgg gaggcattag tccgagtgtt atagccttag
     1441 tctcatgccc cgccactatg ataataaaaa agtaggaaga gagtgtgata gcgatggacc
     1501 ccacaattga tagccacttg tcaatattaa aagagcgtat cagtatgtag aatcctacaa
     1561 aatatcctat tattatccct attaccgaat tgaaaaagag gtggacaatg ttgtggaaaa
     1621 aatagagaaa cttgaacatg aatccttcga aaccactctg cgggggaaag ttgcttatct
     1681 gataagccgg catacccgag aacatggacc cgctccaatt tgaaacattt cctgtcttgt
     1741 tataatattc atacacttcc tgcgccatcc ccttccactg gtaaacatcg ccttgcctga
     1801 gcaccttacc tgaaagaaca gggtaacagt atactactgc taaaaggata aaaatagcgg
     1861 ctgcaaccag atatggcagc aattttttca attttccaaa aatattcttc atagtgataa
     1921 atattacaga ttggtaatca ctctgacggc agcaggaatt acctccacac ggatgggact
     1981 caatccaacc tcttctccgt caacctctac aaatgactca ggttcagccg aaatttcaag
     2041 aacccgcgcc tgggtgtgaa ttacctcatt tacatcatgg atttttcccg taaatagagt
     2101 gggaatctta cgcaaaatct ttgatttcgg aatatctttg ataactgtca aatcgatcag
     2161 accatcatcg aatttggcat taggggtctg tatcatacca ccacctgaat acctccctat
     2221 tcccatggca actgaaaaca catctccctc gtagaaaaga ttaccgtcag ccactatact
     2281 aaacttgcgt gcatcatatc ccataaaacc cttgagaaga cacttgagat atagggcatc
     2341 acttgtggcc tggccctcct ttttttccag gccaaaattg tggcaaacct gggcatcgaa
     2401 gcctaatccg ccaatattga ccatatagga gataaaaggg gcgccatcca taaaagactc
     2461 tacttttgca acatcctgca gcacgctctt accccttgcg atcacatccg taacatcatc
     2521 gtaccccctg gggatctcat gcaaacgagc ccagtcattg ccggagccaa caggaattat
     2581 ggctagggta acctgatttg atggaacttt attctgtaac ataatacctg aaagcacttc
     2641 atgtaacgct ccatctccac caacgacaat catctgacga ataccctttt ttagggcttc
     2701 agcagcaatt tcacgcgcat gaaaacgata ttcggtaaaa gaaaccgagt atgcgatgcc
     2761 tctggcttcg aggcgggccg ctatttcagg ccattcacga cccgctttgc ccccaccgga
     2821 catactgctt acaatcactt tccagaagtc tgccatactt gctcaaaaat cattttctga
     2881 caaaattaac ataaatacct ttcctaagaa aaagaatttg taaatttgta ttttatttag
     2941 accaaatttg gagcatctat gggaaaaatc attgcaatag ccaatcagaa aggtggagtt
     3001 ggaaaaacca ccacagctat taaccttgcg gcctcattgg ctgtgctgga gaggaaaacc
     3061 ctgctgattg atgcggatcc tcaggccaac actacctctg gactcaattt caacccggac
     3121 gcagattcag aaaacactct ctacgaggtt cttatcggtc aaaaggatat tgagcaaacc
     3181 attatgccca cagagctgga ttacctcgat attattacct cccatatcaa cctggtaggc
     3241 gcagaaatag agatgctcaa tgtagaaaat cgtgagtctg tgctgaaaaa ggctcttgca
     3301 gtcctgcgtg accgttacga atacatcatt ctggattgtg caccctcact tggactgatt
     3361 acagtcaacg cattgactgc tgccgactct gtgatcatac ctgtacagcc tgagtttttt
     3421 gccctcgaag gacttggcaa gttgcttaat accattaaac ttgtccagaa tcgtctaaat
     3481 ccggctctaa acatagaagg attcctgttt acaatgtttg acagtcgcct tcgcttacac
     3541 aatcaggtgg tggaagaggt aaagacacat ttcaaggaga tggtcttcag cacgattatt
     3601 gtgaggaaca ttaggttgag cgaagctccc tccttcggca agcccgcaat cctatatgat
     3661 gtaggctcac ccggagcatc caattacctg gagctggcta aggaaataat caaaaagaac
     3721 cagtcatggc aaaaagagaa gcattaggaa gaggactcgg agcactatta ggagatgccg
     3781 gcgagatccg acaacctgaa tacagacctg tatctccctc agaaaggagc aatccggcat
     3841 ctatttgtga ggtgcctctg agccaaatta tacctaatcc ggcacaaccc cgcctctcgt
     3901 ttgacgaaga gggcctgttg gaacttgcag attcgattcg cacactcgga attattcaac
     3961 cccttacgct tcgcaagatc ggcccttctc agtaccagat tattagcggc gaaaggcgct
     4021 tcagggcagc caagttggct caccttcaga cagtccctgc ctacattcgc gaggcagatg
     4081 atacttcgat gctcgaaatg gctattgtgg agaacataca gagggaagat ctggatgcca
     4141 ttgagactgc actcggattc caaaggctaa tggaggaatg taatctaact caagaggaga
     4201 tgtcctcaag gattggcaag aaaagggcta ccgtagcaaa ttatctccgc ctgctgaagc
     4261 tccccgtaga gatccaaaag gctgtgaaaa ccggtaaaat aagcgtaggc catgccaaag
     4321 cattgctctc gctggacaac catgcacaac agatcgaact atgtgaactc accatacgca
     4381 aagacctcaa cgtgcgtcag ctcgaagaaa aagtacgtca aagctccatc ccggcacccg
     4441 tgcagcagag caataatcgg caggacaatt atcctgaaag ctactaccgt cttgccgaca
     4501 ttgtaggaaa atattttgat agaggcgtct ccttcaaacg caccgacaat ggaggtggaa
     4561 caatgacagt acgttttaag aatgacaacg agctcgatgc tttcctaaag gttatagaag
     4621 ataaacagtt atagcttaca tagtgaagag atctcttgcc gtcatatttt tactcatagt
     4681 tttccatttc ggcgctgaag cacagtttca gcgttcaggt aacgttacta tgggtcaaat
     4741 gggaggccct ccgggtgcca atatgcagag agatacacaa gtcaatccgg aaagtgatgt
     4801 caaagccgat actaccgccg gcttctcaat gaaggcgctt attcgcggtt atatgggtag
     4861 agatacactc aagcccggtt atatgctcct gggaactgct gttttaccgg gtgccggtca
     4921 gatttataat aaacaatggt ggaaagtccc tattatttac ggaggtatag gagccggaat
     4981 ctataccggt gtttccaata atctaaaata ccaggaaaca ggtgaccaaa agtacaaact
     5041 ctaccgcaat cttgggtata tcagcgccgg acttacctac tggggttcac tactggatgc
     5101 aaccatttca tacaagacag aggtacctgt acccgtcgct gcaaaatcta ccatatactc
     5161 ggcacttcta cccggcctgg gacaggcata caacggcgat tactggaaaa tacccatctg
     5221 gtggagcgga ttcgtagcat gtggatactt tttccaccaa aatgatatcc agtataagcg
     5281 cttcaagtat atttgcaaaa tggacaatga ccctaatagc ggctacatag gaaatattac
     5341 atccggccag gcggaatggt acagagatac ctacagaaga tttcgggatt actcggtcgt
     5401 ggcatttgta cttgtgtatg cactcaatat catagatgcc aatgtatttg cacatatggc
     5461 ggattttgac gtgagcgaca atttagctac acttcagttt gagcctgcca taatccagcc
     5521 gcttactcct tactattctc caacatacgc tccaatgcat tcattcggac ttcaaatgac
     5581 cctcaatttc taattttatg aaagcaagaa ccttaatttc cattgttatt ctcctcactt
     5641 ccctgttaag tttcctgccg gatgcaggag cacagatcag atccagaaaa cagattatag
     5701 aggatcttac acgcagactg gattcgcttc agaatgctta tgactctctg gcattcgaat
     5761 acaaccttct tgctgaacct gtttcggaag atgtagaagt ggaggctacc atggagaagg
     5821 agtttaaaac tgaaacttat acgcctgaaa gcattgactc tctccgcagt gtgtggtatt
     5881 tgcacaaaaa ggccgaagca ttcaatccgg actttgaaat gctcgaaagg gataccctca
     5941 ggtcatccat tcctgactct gtttatattg aaaggcta
//
LOCUS       DAOSIE010000069         5963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_12007_length_5963_cov_3.403013, whole genome shotgun sequence.
ACCESSION   DAOSIE010000069 DAOSIE010000000
VERSION     DAOSIE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12007_length_5963_cov_3.403013"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLI69_03230"
     CDS             <1..511
                     /locus_tag="PLI69_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOG41534.1"
                     /translation="AALIIFCFCADAQKVAYINTEVILETIPEYQAAQIELNELSDKY
                     KAIIEAEIGEIDQLYQRYQQVKNSLTPAQRTAREQEIISKEQVVKNKQRIYFGEDGIM
                     AKRSEELIGPIQARVNSAIETVATMDDFAIIIDLAITPGVVYKNSKYDLTERVLQLIQ
                     IMDIKNIKQ"
     gene            518..1069
                     /locus_tag="PLI69_03235"
     CDS             518..1069
                     /locus_tag="PLI69_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOG41535.1"
                     /translation="MKRITIIFFLLAGLGFSAKAQELKIGHINTQEVIYLMAEMDTVR
                     MRLQVIQTDLIETMQGMEDEYQAKYTDYNRKQATWTPAVKEAKEKEIQDLIARLQQFQ
                     QTAQQELGQQEQELSAPVYQKAQIAINKVAKELSLTYVFNTAAGAIIYMDEAASVDLL
                     PLVKKELGIPEEKVTPTQLPQQQ"
     gene            1128..2960
                     /locus_tag="PLI69_03240"
     CDS             1128..2960
                     /locus_tag="PLI69_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOG41536.1"
                     /translation="MQHKVIDMQNVVIRFAGDSGDGMQLTGTLFADASAIFGNEISTF
                     PDYPSEIRAPQGTVSGVSGFQVHIGSVEVNTPGDFCDVLVAMNPAALKANAKWTKPTA
                     TIIIDVDAFDEAGIKRAGFATLDPFAELKIEDRNIIPSPITTLTQESLKDSGLDNKAI
                     LRSKNMFTLGMCFFLFNRSLDHTYDYLEDKFKKKPQLIEANKKVLFDGYNYASNIHAI
                     PNTYMVQPAPQEKGVYRNINGNQATAWGLIAASEKSGIPLFCGSYPITPATGILEELA
                     IHKNLGVKTMQAEDEIAGICTAIGAAYAGNFAVTTTSGPGLSLKSEALGLAMITELPL
                     VVVDVQRGGPSTGIPTKTEQTDLAQALYGRNGECPIAVMAAHSPSHCFDAAFYAGKYA
                     MEHMMPVILLTEGFLGNGSEPWKIPSMKDYPEIKPPIIDSVEGAYKPYHRDPVTFVRK
                     WALPGKAGLEHRVGGLEKNPDGVISSDPINHEKMCAARAEKVARMADYIPKQKVIGAD
                     KADVLVVGWGGTFGHLYSAVQQMQSEGKSVALAHFDYINPLPKNTAEVFARYKKILVC
                     ELNSGQFVDYLRAKLPDFKYLQYNKVQGQPFIVKEIVDAVNNIL"
     gene            2980..3996
                     /locus_tag="PLI69_03245"
     CDS             2980..3996
                     /locus_tag="PLI69_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOG41537.1"
                     /translation="MAYTAQDFKSNQEVRWCPGCGDHAVLASIHRALPEVCEALGYTK
                     ERIVFVSGIGCSSRLPYYMNTFGFHGIHGRATAISTGMKVANPKLSIWQATGDGDALA
                     IGGNHFIHAIRRNIDINIILFNNQIYGLTKGQYSPTSKLGTITKTSPYGTVENPFNPG
                     ALALGAGSTFFARSLDVELKLSQDILVAAAKNDGTSVVEMLVNCVIFNDGAHASISNR
                     EYRADRTIILQHGEPMIFGKNKDKGLIFRNRKLEVVTIGQDGIGVDDLLVHDSHCKEV
                     GIHNLLVNMKYPDYPVALGIIRDVKENTYDDNVRDQLKEVEAKSTIKCMDDLLHSGAT
                     WVVD"
     gene            4059..5405
                     /gene="gdhA"
                     /locus_tag="PLI69_03250"
     CDS             4059..5405
                     /gene="gdhA"
                     /locus_tag="PLI69_03250"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOG41538.1"
                     /translation="MKVAEIMANLEKKYGHEKEYLQAVHEVLESIEEVYNQHPEFEKA
                     KIVERMVEPDRVFTFRVAWVDDKGDVQTNIGYRVQFNSAIGPYKGGIRFHPAVNVSML
                     KFLGFEQTFKNALTTLPMGGGKGGSDFDPVGKSNGEIMRFCQAYMLELWHCIGPDQDV
                     PAGDVGVGGREIGYFVGMYSKLARQYNTGVLTGKGATWGGSILRPEATGYGALYFTEH
                     VLAKAGKNIKGKTVAISGFGNVAWGAAGKATELGAKVIAISGPDGVCSIPNGITQEMI
                     DYMLVMRSSNRNKVQDMADKFPGAAIFTPGKKAWSIKCDIALPCAFQNELNGDDAKEL
                     IANGTWCCCEVSNMGCTPEAIAAFQGTKGFLFAPGKAVNAGGVATSGLEMTQNASHIS
                     WSGKEVDEKLHFIMESIHAQCVKFGKRKDGTVDYVKGANIAGFMKVAQAMLEQGVI"
     gene            5706..>5963
                     /locus_tag="PLI69_03255"
     CDS             5706..>5963
                     /locus_tag="PLI69_03255"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG41539.1"
                     /translation="MKRIRLILTTLLLVVSATAFAQQTIQVKGVVKDAATGEGIPFAA
                     IQLKGTMTGAATDAEGVYTISAPGNGVLVFSSVGYQTAEIPI"
BASE COUNT         1724 a         1284 c         1391 g         1564 t
ORIGIN      
        1 tgccgcactg ataatttttt gtttctgtgc cgatgcacag aaggtggctt atataaacac
       61 cgaggtgatc ttggagacca ttcctgaata tcaggcagca cagatagagc tcaatgaact
      121 ttcagacaag tacaaggcta ttatagaggc cgaaattggc gagatagacc aactctatca
      181 gagatatcag caggtaaaga actcactcac tccggctcag cgtactgcga gggagcaaga
      241 gattatatct aaagagcagg ttgtaaaaaa caaacagcgc atctacttcg gagaggatgg
      301 tattatggct aaaaggtcgg aagaactgat cggacctata caagcgaggg tcaactctgc
      361 aatcgaaacc gtagctacca tggatgattt tgccataata attgacctgg caatcactcc
      421 cggtgtggtg tataaaaact ccaaatatga tcttaccgaa agagtgttgc aattgattca
      481 aataatggat ataaaaaaca taaaacaata aaaacagatg aaacgtatta caattatttt
      541 tttcctcctt gccggacttg gattctcggc caaggctcag gaactcaaga tcggtcatat
      601 aaatactcag gaggttattt acctgatggc cgaaatggat accgtgcgta tgaggcttca
      661 agttattcag actgacctta tagagactat gcagggtatg gaggatgaat accaagccaa
      721 gtataccgat tacaaccgta agcaggctac atggactccg gctgtaaaag aggccaagga
      781 aaaagaaatt caggacctta tagcacgtct gcagcagttt cagcaaactg cacagcagga
      841 gcttggacag caggagcagg agctatccgc acccgtttat caaaaggcgc aaattgcaat
      901 aaataaagtt gcaaaggagc tctcgcttac ctatgttttc aataccgctg ccggtgccat
      961 catatatatg gatgaggctg catcagttga tctgcttcct cttgtgaaga aggagctcgg
     1021 tattccggaa gagaaggtga ccccaacgca acttccacag cagcaataat aattaagcat
     1081 ttacaattca tcaattcaat atttacataa aaaaccattc ttattttatg caacacaaag
     1141 ttattgacat gcaaaatgtc gtaattcgtt ttgctgggga ctccggagac ggaatgcagc
     1201 ttacaggtac cctgtttgcg gatgcgtccg ctatttttgg aaatgagatt tccacatttc
     1261 ccgactatcc ctccgagatc cgcgctcctc agggtacggt atccggagtt tcgggatttc
     1321 aagttcacat cggaagtgtt gaagtaaaca ctcccggtga cttctgtgat gtgctggtgg
     1381 caatgaatcc ggctgcacta aaagcaaatg ccaagtggac caaacccact gcaaccatca
     1441 tcatagatgt cgatgccttt gacgaggcgg gcattaagag agcaggtttt gctactcttg
     1501 atccatttgc cgaacttaaa atcgaggaca gaaatatcat tccttctccc atcactaccc
     1561 tcacgcaaga gagtctcaaa gatagcggtc ttgataacaa ggctattctg cgtagcaaaa
     1621 atatgttcac tctcggtatg tgtttcttcc tcttcaacag atcgcttgat cacacatatg
     1681 attaccttga ggataagttc aagaagaaac cacagttgat cgaagccaac aaaaaagtgc
     1741 tattcgacgg ctacaattat gcaagtaata tccacgcgat tccaaatacc tatatggttc
     1801 aacccgctcc acaagagaaa ggtgtatatc gcaatatcaa tggtaaccag gctacagcat
     1861 ggggtttgat agcagcatct gagaagagtg gtatacccct cttctgtggt tcctatccga
     1921 tcacaccggc tacaggtatt ctggaggagc tggcaataca taaaaatctt ggcgtgaaga
     1981 ctatgcaggc tgaggatgag attgcaggta tttgtactgc cataggagca gcctatgcag
     2041 gcaactttgc tgtaacgacc acttcaggtc ccggcctttc tctaaaatcc gaggctctgg
     2101 gtcttgcaat gattaccgaa ctgccgctgg ttgtcgtaga tgtccaaagg ggcggtccat
     2161 caacaggtat tcctaccaag acagagcaaa cagacctggc tcaggctctt tatggtcgta
     2221 acggtgagtg tccgatcgcc gtaatggcag cgcactctcc ctcgcactgt tttgacgcag
     2281 cattctatgc cgggaaatat gccatggaac acatgatgcc tgtgattctc cttaccgaag
     2341 gctttctcgg caacggttct gaaccttgga aaatcccttc tatgaaggat tatcctgaga
     2401 tcaaaccacc aataattgat tcagtggaag gagcttataa gccttaccat agagatcccg
     2461 taacatttgt tcgtaaatgg gcattgcccg gaaaggccgg tctcgagcat agggttggcg
     2521 gtctggagaa gaatcctgac ggagtaatct cttcggatcc catcaaccat gagaagatgt
     2581 gtgcggcaag ggctgaaaag gtagcccgca tggccgatta catacctaaa cagaaggtta
     2641 ttggagcaga taaggcagat gtgcttgtag taggctgggg tggcactttc ggacatcttt
     2701 acagcgctgt acagcagatg cagtcagagg gaaagagcgt agctcttgcc cattttgact
     2761 atatcaatcc ccttcccaag aacaccgccg aggtatttgc acgctacaag aagattcttg
     2821 tatgcgagct taactcaggt cagtttgtgg attatctcag agcaaaattg cccgatttca
     2881 aatatctgca atataataaa gtccagggac agccatttat tgtgaaggaa atcgtggatg
     2941 cagtcaacaa tattctttaa tctttgaaaa tatactacta tggcatatac agcacaagat
     3001 tttaaaagta atcaggaagt aaggtggtgt ccgggctgcg gagaccacgc agttttggca
     3061 tccatccaca gggctctccc cgaagtttgt gaagcgttgg gttatacgaa ggagagaata
     3121 gttttcgtat caggtatcgg ttgctcttcc agattgccct actatatgaa tacattcgga
     3181 tttcacggca tacacggtcg cgcaacagcc atttccacag ggatgaaggt tgccaatccc
     3241 aaactcagca tttggcaggc aacaggtgat ggtgatgcac ttgctatcgg tggcaatcac
     3301 ttcattcatg caatccgcag gaatatcgat atcaacataa tcctattcaa caatcaaatt
     3361 tacggtctca ctaaaggaca gtattctcca acatccaaat taggtacaat taccaagaca
     3421 tccccttacg gcacggttga gaatcccttc aatccgggag cacttgcact gggtgccgga
     3481 tcaacattct tcgcacgcag tctggatgta gagctcaagc tcagtcagga tattcttgta
     3541 gcagctgcca agaatgacgg tacttcggtg gtagaaatgt tggtcaattg tgtcatcttc
     3601 aatgatggtg ctcacgcctc aatttccaat cgcgagtatc gtgctgacag gactatcatt
     3661 ctccagcatg gagagcctat gatatttgga aagaacaagg acaagggtct gatattccgc
     3721 aacaggaaac tggaagtggt tactatcggt caggatggaa ttggagtgga tgacctgctc
     3781 gttcacgatt cacattgcaa ggaagtcggc atccacaact tgcttgtcaa tatgaaatat
     3841 cctgactatc ctgttgcttt gggaatcatt agagatgtaa aggaaaatac ctatgatgac
     3901 aatgtaaggg atcagttgaa ggaggtggag gcaaaatcca ctataaagtg tatggatgat
     3961 ctgcttcaca gcggtgcaac ttgggtagtt gactaaccaa ttggaaaatt tttactatat
     4021 taacgcaaat atcaacctta taaattaagt taaaaattat gaaagtagct gagattatgg
     4081 ccaatcttga gaaaaaatat ggccatgaaa aagagtactt gcaagccgta catgaggtgc
     4141 ttgagtcaat cgaagaggtt tacaatcaac atccggaatt cgaaaaggca aaaatcgttg
     4201 aaaggatggt ggagcctgac cgagttttca ctttccgggt tgcctgggta gacgataaag
     4261 gcgatgttca gacaaacatc ggctatcgtg tacagttcaa tagcgctatc ggtccctaca
     4321 agggtggtat ccgtttccat cctgccgtga atgtttcaat gttgaaattc ctcggtttcg
     4381 agcagacctt caagaatgca ctaaccactc ttcccatggg tggtggcaaa ggtggttccg
     4441 acttcgaccc cgtaggcaaa tccaacggtg aaatcatgcg tttctgccaa gcatatatgc
     4501 ttgagttgtg gcattgtatc ggccccgacc aggacgttcc tgccggtgac gtaggtgttg
     4561 gcggacgcga gatcggctac tttgtcggta tgtactctaa acttgcacgc cagtacaata
     4621 caggtgttct aaccggcaag ggtgcaacct ggggtggttc tatccttcgt cccgaggcta
     4681 caggttatgg agctctttac tttaccgagc acgtacttgc aaaagcaggt aagaatatca
     4741 aaggcaagac ggttgctatc tctggctttg gtaacgtagc atggggtgca gcaggtaagg
     4801 ctaccgaact tggtgcaaaa gtaattgcaa tctcaggtcc ggacggcgtt tgctccattc
     4861 ctaatggtat cacccaggag atgattgact atatgctcgt tatgcgttct tcaaaccgca
     4921 ataaggttca ggatatggct gacaaattcc ctggtgccgc tatttttact ccaggaaaga
     4981 aggcttggag catcaagtgc gatatagccc ttccttgcgc attccagaat gagcttaacg
     5041 gtgacgatgc gaaagaactt attgctaatg gcacatggtg ctgctgcgag gtttcaaaca
     5101 tgggatgtac tcctgaggct attgctgcat tccagggcac aaaaggcttc ctctttgccc
     5161 ccggcaaagc tgttaatgca ggtggtgtag caacttcagg tcttgagatg actcagaatg
     5221 cttctcacat cagctggtca ggcaaggaag ttgatgaaaa actccacttc atcatggaaa
     5281 gcatccacgc tcagtgcgtg aaattcggta agagaaaaga tggtacagta gactatgtta
     5341 agggtgccaa catcgccgga tttatgaagg ttgctcaagc catgcttgaa cagggtgtta
     5401 tctaatcttt taatagctcg aaagagatta tattttgtca ggggtggcca tgaggtcacc
     5461 cttgattttt tacttataat atttttaaga tactccccgt attgattaca tttttaacaa
     5521 aaggagtaca ttttctttat aaattgttat tttcccctga tttttaagga gtttattatt
     5581 tgaaatgtga ttttttttta tttactttgc aggatgaaat aatatgcacg actggcctct
     5641 gaccggtgtg cacaaataag agaataatat ataaacacca attaatcaac caaaaattta
     5701 tctacatgaa aagaatcaga ctaattctga caactctctt gcttgtagtg tctgcgactg
     5761 catttgcaca acagacaata caggtaaaag gtgttgttaa agatgcagct accggagagg
     5821 gtattccctt tgcagctatc cagctcaagg gtaccatgac cggagcagca acagatgctg
     5881 aaggagttta caccatatcc gctcccggca acggtgtact cgtgttcagc tcggtaggtt
     5941 accagacagc ggagattccc att
//
LOCUS       DAOSIE010000070         5949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_12049_length_5949_cov_3.150662, whole genome shotgun sequence.
ACCESSION   DAOSIE010000070 DAOSIE010000000
VERSION     DAOSIE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12049_length_5949_cov_3.150662"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="PLI69_03260"
     CDS             complement(<1..1491)
                     /locus_tag="PLI69_03260"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG41540.1"
                     /translation="MKQINITLVAIAALFISNALSAQVPLKIVIGGQDTVYNQKHTIV
                     CITDPGNVAAINENQLKVYRTGAFGTELDLKRGNNKIEITLFKGKNRRIETLNVYYDP
                     DAKKPVRPEPKMTLEEAKAILEKSKYKEELFYATSLEGAYLQFGDGDDRLGGSKMGYV
                     DQEILFKVVASVGDLYKVQLSQNKFAYIPREYLERSEERTSQVNTGSWSVSNLGKADR
                     VSISLPKRLPYVSWTQLDPTTICIDLYGATNNSNWITQYRGLQMISYVDYRQVDSDVF
                     RVILRLKHKYSWGYKIGYEGNNLIIDVRHTPELSLKGLRVGLDAGHGGTALGAVSITG
                     IREKDVNLDIVNMLKGALEAKGATVVLSRSGDYDISMSERKRIFNENNVHIALSIHNN
                     AGGSPVNPMGSSTYYKHITNRDFAETMLERLLELGVPNFGLVGNFNFSLAAPTEYPVV
                     LLELLFMSSIWDEERLVDPDFRKKMVNNIVLGLEDYLEKVDSSIEEP"
     gene            complement(1588..2529)
                     /locus_tag="PLI69_03265"
     CDS             complement(1588..2529)
                     /locus_tag="PLI69_03265"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOG41541.1"
                     /translation="MKKIALYTLLLLTTLPLLAQVTEQYEQKLYRGKQQEILSHIADD
                     MAKGRASGTITKQAIEQYIVRRFKEYGLKPYKWHYTQSSRCSDTIIARNVVGIIPAVV
                     QTDKYVVVGAHYDHIGEINGKIYNGADDNASGVTALINLAEMFSEMKKDGRGPAKNII
                     FVAFDGKEFNMCGSEHFVQNLPFPARNITCMVNLDMIGTDLEPVGFRRDYLIVLGEER
                     LDPKYRGILGYLSRRKNYDMDLDLTFYGSKDFTQIFYRMSDQYSFAKAGIPALLFTSG
                     IHKHTYKPSDKSDIINYDLLRKRTLLIFNFLFRICEY"
     gene            2571..4571
                     /locus_tag="PLI69_03270"
     CDS             2571..4571
                     /locus_tag="PLI69_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG41542.1"
                     /translation="MFKKAIFIFLLLFITALPVWCQYDLDHFYYRGRQALIDGKYSNA
                     IESFNILSRLDSTIFEAYFFRGIAKYNLGDLVGAQMDFDKTLHFNPIYTPAYHYRAIT
                     LSRIGKYDLALKDLEEAVDLRPGYTGLYFSRGVTYFLSQQFEKAVKDFNMFIKKEPRE
                     SNAYLNRGASYLYLGDTTKALEDYHKAISIDRYDPEAYVRRSRIYALRHDLEPALEDL
                     NTALRLDTANTFAYFNRAIIRHEMKDIEGALADLDKVLENEPGNALTLYNRAIIRAQV
                     GDYRSALDDYDRVLAINPNNVLAYFNRAAVFMEMGRYRDAMDDYSKAINLYPDFAKAY
                     MNRAYAKSQLGQYNSSRQDYEIAQRKIEEYRARTSDSTGYAAFADTTRKYDHLLSLDA
                     DFAKKDFDNELLQYRDVDIRLKPLYRFAAVVPGETRQEGNILINRKFDDESLDRFISA
                     SPIAVKFESSKGGYLPDAQAIRDEVNRLLRSDRTDINLFAKALLETDNKQFNTAMVCY
                     DEAIQKSPDNIFYYINRGALQAEMIDFISSIENNVPVLTLDNDNTLKTRVQEQVRHTY
                     DYTPAIHDMNKAASIDPSFPYIYYNLGNLYCLSDDLPESIVQYSKAIELYPNIGEAYY
                     NRGLVLIYLKDKEKGCMDLSTAGELGIQDAYSVIQKYCRSED"
     gene            complement(4574..5038)
                     /locus_tag="PLI69_03275"
     CDS             complement(4574..5038)
                     /locus_tag="PLI69_03275"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 family protein"
                     /protein_id="HOG41543.1"
                     /translation="MFKRFQTYLIIAAGLLIGSMFFSNMCYGVIPTDAPSEQWERITI
                     SFFERSQFLIMAFVGFSLCVIALFYHKKRLLQIRICLLDSIIIFAFQIWILVEFMKLR
                     PFYSLSVPSLFPIIAIILLVLAIRHIAKDEATAMFSKTVRTNNTGNKKGRKK"
     gene            5119..>5949
                     /locus_tag="PLI69_03280"
     CDS             5119..>5949
                     /locus_tag="PLI69_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOG41544.1"
                     /translation="MSTLPIAPSELIINPDGSVFHLHLLPEELADTVILVGDPGRVEL
                     VASFFEEGSIRVDKSSREFRTVTGRYNGKEVSVLSTGIGTDNIDIVVTELDALANIDF
                     YTRCVKKDHKRLTLLRIGTCGAVQPDIPLGSYIFSEYSIGFDGLINWYAGSGKVVDLE
                     MESAFVEHMNWPRRLPAPYFIKSSGRLIPMFKNKSIMGMTISAPGFYGPQGRSVRLPL
                     TFDNLIGDLESFRYGDRRITNIEMESSALSGLSALLGHDAATICLVIANRYAKESRPD
                     Y"
BASE COUNT         1625 a         1302 c         1310 g         1712 t
ORIGIN      
        1 gggctcttcg atggaggaat ccactttttc gagataatcc tcaagcccaa gtacgatatt
       61 attgaccatt tttttccgga aatcggggtc gacaagcctc tcttcatccc agatactgct
      121 cataaagagg agttccagca ataccaccgg gtattcagta ggtgccgcta atgaaaagtt
      181 gaagttgccc acaagtccga agttagggac tccaagctcc aggagtctct cgagcattgt
      241 ttcagcaaag tcccggttag tgatgtgctt gtagtatgtg cttgatccca tcgggttgac
      301 cggagatcct ccggcattgt tgtggatgga tagcgctatg tggacattat tttcattgaa
      361 gatgcgtttt ctttcactca ttgagatatc ataatcaccg cttctgctga gtacgactgt
      421 ggctcctttg gcttcgagag caccttttag catatttact atatcaaggt tgacatcctt
      481 ctcccggata ccggtgatgc ttacagcacc aagagcggtg ccgccatggc cggcatcaag
      541 accaactctg agtcctttaa gtgagagttc aggtgtatga cgcacatcta ttatcagatt
      601 gttaccttca tatcctattt tatagcccca ggaatatttg tgtttgagtc ggagaattac
      661 cctgaacaca tcgctgtcca cctgtctata gtcgacatag gagatcattt gcagacctct
      721 atactgtgtt atccagttgc tgttgttagt ggcaccatag agatcgatgc agatggtggt
      781 aggatcgagt tgggtccagg agacatacgg gagacgtttg ggcaaagaga tactaaccct
      841 atcagctttg ccgagattgg atacactcca gctgccggta ttgacctggg aggttctttc
      901 ttcactcctt tcaaggtatt ccctgggaat gtatgcaaat ttgttttgtg aaagctgcac
      961 cttataaagg tctccgactg aggcaactac tttgaatagt atttcctgat ctacatatcc
     1021 cattttagaa ccgccaagac gatcatctcc gtcaccaaat tgcaaatatg ccccctcaag
     1081 agaagtagcg taaaaaagct cttccttgta ttttgatttt tctaaaattg ccttagcctc
     1141 ttccagtgtc atttttggct cgggacgaac cggctttttg gcgtcgggat cgtaataaac
     1201 attgagggtt tcaatacgtc tgtttttacc tttgaatagt gttatttcaa ttttgttatt
     1261 accacgctta aggtccagtt ccgtaccgaa agcgcctgta cggtaaactt tcaattggtt
     1321 ttcattgatg gctgctacat ttccggggtc agttatgcag acaattgtat gtttttgatt
     1381 ataaactgtg tcctgccctc ctataacgat tttcagcggc acttgagccg atagggcatt
     1441 agagatgaat aatgcggcaa tagccacgag agtaatattg atctgcttca ttgtataaag
     1501 attaaatgca tgtaaatatt ttatgcacga tagcattatc gtgtcttata aataacaaat
     1561 atagtgaata atccccaaat ataggaatca gtattcacag atcctgaaga ggaaattgaa
     1621 tatcaataga gtgcgttttc tcagtaagtc gtagtttata atatcagact tatcagaggg
     1681 cttgtaggtg tgtttatgga tgccgctggt gaagagaagg gccggaattc ccgctttggc
     1741 aaaggagtat tgatcgctca tcctatagaa gatctgagta aaatctttgc tgccgtagaa
     1801 ggtaaggtca aggtccatat cataattctt tcttctacta agatagccca aaatacccct
     1861 gtatttgggg tccaggcgct cctcgcccag tacgattaaa taatcacgtc tgaatcctac
     1921 cggttcgagg tccgtaccta tcatatccaa atttaccata caggtgatat tgcgtgcggg
     1981 aaagggcagg ttttggacga aatgctccga accgcacata ttgaattctt tgccatcaaa
     2041 ggctacgaaa ataatgtttt tggccggtcc tcttccatcc tttttcattt ccgaaaacat
     2101 ctcagccagg tttataagcg cagtgacgcc ggaggcgttg tcatcagccc cgttatagat
     2161 tttaccgttg atttctccga tgtggtcata atgagcacct acgactacat atttgtcggt
     2221 ttggaccaca gccgggatta ttcccactac atttctcgca ataatggtgt cactacatct
     2281 gctactttgt gtgtaatgcc atttgtaggg cttaagacca tactctttaa acctacgtac
     2341 tatatattgt tcgatggctt gcttggtaat tgtgcctgat gcccgccctt tcgccatgtc
     2401 atcggcaatg tgagagagta tttcctgctg tttcccgcgg tacagctttt gctcatattg
     2461 ctccgttacc tgtgccaaaa gcgggagtgt cgtcaacaga agtaatgtat atagtgcgat
     2521 ttttttcatt ttttgtccaa gcaaagtgca aaagtactat ttttgtcaat atgttcaaaa
     2581 aagccatttt tatcttccta cttcttttta tcaccgccct accggtctgg tgccagtacg
     2641 accttgacca tttttactac aggggaagac aggctctgat agatggtaaa tactccaacg
     2701 caattgagag tttcaacatt ctttcccgcc tggacagcac catatttgag gcttatttct
     2761 tcaggggaat tgcaaaatac aatctcggtg accttgtagg agcccagatg gactttgaca
     2821 aaaccctcca tttcaatccc atttacaccc cggcatatca ctaccgtgca attaccctca
     2881 gcaggatcgg taagtacgac ttggcgctca aagaccttga agaggccgta gacctgcgtc
     2941 cgggctatac cggcctctat ttcagcaggg gagtcaccta cttcctttcg caacagttcg
     3001 agaaggcggt aaaagacttc aatatgttta tcaaaaaaga gcctcgtgag agtaatgcat
     3061 acctcaacag aggggcatct tacctctatc tgggtgatac aaccaaagct ctggaggatt
     3121 accacaaagc gatctccata gatagatacg acccggaggc atatgtacgc aggtcgagaa
     3181 tctatgctct ccggcatgat ctcgaaccgg cactggagga tctgaatacc gcactgagac
     3241 tcgatactgc aaataccttt gcatatttca acagggcaat aatccgacat gaaatgaagg
     3301 atattgaagg ggcgcttgca gacctggata aagtgctgga aaatgaaccg ggcaatgccc
     3361 ttacactcta taacagggca attattcgag cccaggtggg agattatcgc agtgctctcg
     3421 atgactacga tagggtactg gcaattaatc cgaacaatgt gttggcctat ttcaaccgtg
     3481 ccgcagtctt tatggaaatg ggccgttacc gtgatgctat ggatgactac tccaaggcga
     3541 tcaatctcta tccggacttt gcaaaggcct atatgaatag ggcctatgcc aagagtcaac
     3601 ttggacaata caactcttcg cgtcaggatt acgaaatagc tcaaagaaaa atagaggaat
     3661 acagagccag gacatcagat tctaccgggt atgccgcatt tgccgatact acccgtaaat
     3721 atgatcatct gttgtctctt gatgcagatt ttgccaagaa agattttgac aatgaactgc
     3781 tacaatacag agatgtggac attcggctaa aacctctcta taggtttgcc gcggtggttc
     3841 cgggagaaac tcgccaggag gggaacatac tgattaaccg caagtttgat gacgaatcgc
     3901 tcgatagatt tataagcgca tctcccattg cagttaaatt tgaatcttct aaaggaggat
     3961 acctgcccga tgcccaggct atccgggatg aggttaatcg tctgctccgt agcgaccgta
     4021 ctgatataaa tctctttgcc aaggcattgc tcgagacgga taacaaacag ttcaatactg
     4081 ctatggtctg ctatgatgaa gccattcaga agagtcctga caatattttt tactacatta
     4141 atcgaggtgc cctgcaggca gaaatgatag acttcatatc atcaatagaa aacaatgtcc
     4201 ccgtgctgac gctcgataat gacaatactc taaaaacgag agttcaggag caggtacgtc
     4261 atacctatga ctatactccg gccatccacg atatgaataa ggcggcatcg atagatcctt
     4321 ccttccctta tatttactac aatctgggca acctctattg cctctccgac gaccttccgg
     4381 agtcgatagt acagtattca aaagcaatag agctctatcc gaacatcggt gaagcctact
     4441 acaatcgcgg tttagtgctg atttatctta aagataagga aaaaggttgt atggatctga
     4501 gtacagcggg ggagttgggc attcaggatg cctatagcgt tattcagaaa tattgcagga
     4561 gcgaagatta gggctacttc tttctacctt tcttattacc ggtattattc gtacggacag
     4621 tttttgaaaa catcgccgta gcttcatctt ttgctatatg gcggattgcc agcaccaaca
     4681 ggataattgc aattatggga aatagtgacg ggacagaaag ggaataaaag ggtctgagtt
     4741 tcatgaattc caccaatatc catatctgga aagcaaaaat aataatggag tccagcaagc
     4801 atatacgtat ctgtagcaga cgttttttgt gataaaagag tgcaatcaca catagggaaa
     4861 agcccacgaa tgccattatc aagaactgac tcctttcaaa aaaactgatg gtaattcgtt
     4921 cccattgctc cgaaggggca tcggtaggaa taacgccata acacatattg gagaaaaaca
     4981 ttgagccgat cagcaatcct gctgcaataa taagatatgt ctggaatctt ttaaacatac
     5041 tcttggatgt gtggattgat ttgacaaatt taggaaaaaa gtcctatatt tgagttttaa
     5101 acataaaaaa tgacatatat gtctacttta ccaatagcac cttctgaact tattataaat
     5161 cctgacggat cggtgtttca ccttcatctt cttccggaag agttggctga tactgtcata
     5221 cttgttggag accccggtag ggtagaactt gtagcctcat tctttgaaga gggctcaatt
     5281 agggttgaca aatcatcacg agagttccgt acagtcaccg gccgctataa tggaaaggaa
     5341 gtctctgttc tctccaccgg tattgggact gacaatatag atattgtggt taccgaactg
     5401 gatgctcttg caaatatcga tttctatacg agatgtgtca aaaaggatca caagcgattg
     5461 acactgcttc gtatcggtac ctgtggcgct gtccaaccag acattcctct tggcagttat
     5521 atattttcag agtattccat aggatttgac ggactcataa actggtacgc cggatccggt
     5581 aaggttgtgg acttagagat ggagtctgct tttgtcgagc atatgaattg gccgaggcga
     5641 cttcctgctc cctatttcat aaaatcttcc gggcgtttga ttcctatgtt taaaaataaa
     5701 tcgatcatgg gaatgaccat atctgctccg ggtttttatg gtcctcaggg ccggtcggtc
     5761 agactccccc tcacatttga caatctaata ggggatcttg agtctttccg atatggtgat
     5821 cggaggatta ccaatattga gatggagagt tccgcactat ccggtctttc cgcacttctg
     5881 ggacacgatg ctgccactat ctgtcttgtg atagccaatc gttatgcaaa agagagccgt
     5941 cctgactac
//
LOCUS       DAOSIE010000071         5908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_12173_length_5908_cov_2.740988, whole genome shotgun sequence.
ACCESSION   DAOSIE010000071 DAOSIE010000000
VERSION     DAOSIE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12173_length_5908_cov_2.740988"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLI69_03285"
     CDS             complement(<1..397)
                     /locus_tag="PLI69_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HOG41545.1"
                     /translation="MAEKILMKGNEAIAYAAINCGCDGYFGYPITPQSEVMETLMQEK
                     PWETTGMVVLQAESETSSINMVYGGASCGKKVMTSSSSPGISLMCEGLSYLAGCELPC
                     LVVNVMRGGPGLGTIQPSQSDYFQATKGGG"
     gene            complement(408..629)
                     /locus_tag="PLI69_03290"
     CDS             complement(408..629)
                     /locus_tag="PLI69_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG41546.1"
                     /translation="MAIKGAITVDTEKCKGCGVCIPNCPTGTIALAKEVNSKGYNYLY
                     MANPDNCIGCANCATVCPDAVITVYRVKI"
     gene            complement(659..814)
                     /locus_tag="PLI69_03295"
     CDS             complement(659..814)
                     /locus_tag="PLI69_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41547.1"
                     /translation="MFKKIGDWYRAQSYTTKALIWIGIICIIGIAFRWQAIIEGVTKG
                     FNFYSGK"
     gene            complement(821..2875)
                     /locus_tag="PLI69_03300"
     CDS             complement(821..2875)
                     /locus_tag="PLI69_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG41548.1"
                     /translation="MITKQLISPKSIVVVGGSDDIRKPGGAILENLLSTRFQGNVYVV
                     NPKASGEIRGCRTFLNIDGIPEVDLAILAIPAQFCVSAVETLCYKKNCGAIIIVSAGF
                     AEDSKEGAEYERRIVEMTNDVGAALIGPNCVGVITPAYTGVFTKPIPSLSPMGIDFVS
                     GSGATIVFILELAIQLGLKFASIFSIGNSAQIGVEEMLEYYDQSYVHGKSAPVKMLYI
                     ESINDPQKLLKHAYSLYRKGARVVAIKSGYSEAGSRAASSHTGAMASPDKAVSALFRK
                     AGIIRCYSRMELVTTAAILMFNQVKGRKMAIITHAGGPAVMLTDVLSSNDIEIPTFEG
                     EKAKALLKELYPGSSVSNPIDFLATGTASQLGKIIDACEHDFDVDSMAVIFGNPGLVS
                     GNDAYDTILEKQKVCKKPIFPILTSIVNAKDEIEQFRNKGGITFPEEVIFGSCFARIA
                     NTLEPILSNENPPVDKEKIRRIIDNSDNGYLSPEKTQEILDAAGINRVKELVVSTYPE
                     AIEAAREIGWPLVMKVVGPVHKSDVGGVVLNISDDHTLEMEYKRMMRIDGCIGVLLQP
                     MISGTQLFVGAKREDKFGHLVMCGLGGIYIEVLKDISYGISPISAIEAKEMIRHLRSY
                     KLIEGVRGQEGVNEAIFNETIRRVSALCSCAPEIFEMDINPLMGNQKSVTAVDARIRI
                     EK"
     gene            3077..4336
                     /locus_tag="PLI69_03305"
     CDS             3077..4336
                     /locus_tag="PLI69_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG41549.1"
                     /translation="MGTILLKHATLKGKQKDILIKGNRIAVIADSIPVRAAREIDCTG
                     KSVIPGLINMHTHSAMSLLRGVSEDVPLTEWLNKIWSIEKHLQKEYVYWGARLAILEM
                     IKTGTTCFLDMYWMSPQVAQAVEEMGIRGVLTYVFLDNYDTEKAEKQKSECIRIHGYS
                     KQWHKRCKFGVSIHADYTVSPHTMVWASGFAKEKGLLINTHISETEKEVLADKEKHGK
                     TPVAFYDSLGILDSNVIAAHALWLEDEDIEIFAKRGVTAVHNVNSNLKLGSGCRFRYG
                     DLKSAGVNVCLGTDGAASSNNLDMRESMKTMSLLQKGWTGDTTILPLNELMDVATVNG
                     ARALDLDTGRIEEGALADLVLVETRSEAFIPNINFESNFIYSANSSCIDTVICDGNIL
                     MEGGKVAGEEQLLEKADEMAWKLINAT"
     gene            4333..5163
                     /locus_tag="PLI69_03310"
     CDS             4333..5163
                     /locus_tag="PLI69_03310"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOG41550.1"
                     /translation="MMDQRIKAIYEAADYIRERTGGFVPKVGIILGSGLGKLADSIEN
                     PITIPYAEIPRFSRSTAIGHKGNLILGTIAGKPVCAMQGRFHYYEGYPMDQVTLPVRV
                     MKVLGIEYLFVSNAAGGVNLDYKVGDLMIISDHINRLPNPLVGSNLDQFGPRFPDMTR
                     PYDLNLIRKAENIAEKEGIKLFKGVYLAGTGPSYETPAEYRFFRMIGADAIGMSTIPE
                     VIVARHSDIPVFGISVITNEAHGDFAEDFVNDGDDVVKAADAAAGTMTYLFSKLIGSL
                     "
     gene            5163..>5908
                     /locus_tag="PLI69_03315"
     CDS             5163..>5908
                     /locus_tag="PLI69_03315"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOG41551.1"
                     /translation="MLTTVFPYDSVELSSNGREVIILDQSLLPNQEKFLHLTTAEQVF
                     FAITLMKVRGAPAIGIAAAFGLAMSINRSKCRELATFEKEFLRTKRYLYISRPTAVNL
                     MWALDRMEDCFYKVKNSIDDTDQTVIDIIKNALIAEARSIKVEETRMSVAIAEHGVSL
                     LTHGCGVLTHCNAGHLATSRYGTALSPIYLAHQKGYSPMVYVDETRPLLQGARLTAYE
                     LMKAGIDVTLICDNMSSIVMSQKRVDSVFVG"
BASE COUNT         1531 a         1351 c         1385 g         1641 t
ORIGIN      
        1 gaccgccacc ttttgtagcc tgaaaatagt cactctggct gggttggata gtgcccagac
       61 ccggaccgcc acgcattacg ttgactacca gacagggaag ttcgcagcct gccagatagc
      121 ttaggccctc gcacatgagg ctgatgccgg ggctgctgga tgaggtcata acctttttgc
      181 cgcaggaggc tccgccatag accatattga tcgatgatgt ttcactctca gcctggagta
      241 ctaccatacc tgtagtctcc cagggttttt cttgcatcag cgtctccata acctccgact
      301 ggggggtaat ggggtagccg aaatagccgt cgcaaccaca gtttattgca gcataggcga
      361 tggcttcgtt tcctttcatt aagatctttt ctgccatatc tgtaatttta gattttgaca
      421 cgataaacgg ttattactgc atccggacat acggtagcgc agttggcgca gccgatgcag
      481 ttatccggat tggccatata taaataattg taacctttac tattcacctc ttttgcaagg
      541 gctatagtgc cggttggaca atttgggatg catactccgc atcctttgca tttctcggta
      601 tctaccgtga tagctccttt gattgccata tacttttaag ttttattggt tttaaaaatt
      661 atttgcccga ataaaaattg aaccctttgg tgacgccctc gattatcgct tgccacctga
      721 aagcaatacc gataatgcaa attatgccta tccatatcag tgcctttgtc gtgtagcttt
      781 gggcacggta ccaatcgcca atttttttaa acataattta ttatttttca atcctgatcc
      841 tggcatccac agcggtcacg ctcttctggt tgcccatcaa gggatttata tccatttcaa
      901 aaatctccgg agcacaactg caaagagcag agactcggcg gatagtttcg ttgaatatgg
      961 cttcattgac cccctcctga ccacgtacgc cctcaatcag cttgtagctc ctaaggtggc
     1021 ggatcatctc cttggcctct atggcagaga tgggggagat accataagaa atatctttaa
     1081 gtacctcaat atagatacct ccgagaccgc acatcaccag atggccgaat ttatcttccc
     1141 tcttggctcc cacaaagagt tgagtgccgc tgatcatagg ctggagcagg actccgatgc
     1201 agccatctat gcgcatcata cgtttgtact ccatttcgag agtgtgatcg tcgctgatgt
     1261 ttagcacgac tccacccaca tcgctcttat gcactgggcc caccaccttc ataaccaggg
     1321 gccagcctat ctctctggca gcctcaatgg cctccgggta ggtggagacc accaattctt
     1381 tcaccctgtt gatgcctgct gcatccagta tttcttgcgt tttctccggt gagaggtagc
     1441 cattgtcgct attgtcaata atcctacgga ttttttcctt gtcaaccgga ggattctcat
     1501 tgctgagaat gggttcgaga gtgttggcta ttcttgcaaa gcaagaaccg aagatcacct
     1561 cttcggggaa tgttattcct cctttgttcc taaattgttc tatctcatct ttggcattta
     1621 caatggaggt aaggatgggg aatatgggct ttttacaaac tttttgcttc tcaagaattg
     1681 tgtcgtatgc gtcgttaccg gagaccaggc cgggattgcc gaatattacc gccatactat
     1741 ccacatcgaa atcatgctca caggcatcta taatcttgcc cagttgggat gcagtaccgg
     1801 tggcaagaaa gtctattggg ttggagactg aagaaccggg gtagagttct ttgagaagcg
     1861 ccttagcctt ttctccctca aaagttggga tctctatatc attggaagag agtacgtcgg
     1921 taagcattac ggcaggaccg ccggcgtggg tgatgatggc catcttacga cctttgactt
     1981 ggttgaacat cagtatggcg gcggtagtta cgagttccat gcgactgtaa catctgataa
     2041 tacccgcctt acggaagagc gcactgacag ccttatcggg agaggccata gcaccggtat
     2101 gtgaggatgc ggcacgtgat ccggcttcgc tataccctga tttgatagct actacccttg
     2161 ctcccttacg gtagagggaa tatgcatgtt tgagaagctt ctgaggatca tttatgcttt
     2221 ctatgtagag catcttgacc ggtgcgcttt ttccatgcac gtaggattgg tcataatact
     2281 caagcatctc ctcaactccg atctgagccg aattgccgat ggaaaaaata gatgcgaact
     2341 tcaatccgag ctgtattgcc agctccagga taaatacgat ggtggctccg gagcccgata
     2401 cgaaatcaat acccatgggg gagaggcttg gaatgggttt ggtgaagact cctgtatagg
     2461 ccggcgtgat tactccgaca caattggggc ctatcagtgc ggctcctacg tcgtttgtca
     2521 tctcgactat cctgcgttcg tattccgccc cctctttact gtcttcggca aatcctgccg
     2581 aaacaataat gatggctccg cagtttttct tgtagcaaag cgtttcgacc gcactcacac
     2641 aaaattgtgc gggtatggca agaatggcaa gatcaacttc gggtatccca tctatattga
     2701 ggaatgttct gcagcccctg atttctccgg aagctttggg attaactacg taaacattcc
     2761 cctgaaaccg tgtagaaagg agattttcga gaatggctcc cccgggcttg cggatgtcat
     2821 cactaccccc tacaacgaca atgcttttag gtgaaatgag ctgttttgtg atcatcggtt
     2881 ccaaatttta ccatgcaaag ttataaatta ttatcgaaaa tatcaataat aattcaaaaa
     2941 catcaaataa atatagtttt aattacaaat tgtaaccaat agtcccgaat ttggtgatac
     3001 ttgctaaaca aatgcatcat tattgtgaca atatggatct tttttgttat atttgtcaaa
     3061 ttttagatac taacttatgg gtactatttt gcttaaacac gctaccttga aagggaaaca
     3121 aaaagatatc cttataaaag gtaaccggat tgcggtcata gcagactcca ttcctgtaag
     3181 agcggcgaga gaaattgact gtaccggaaa aagtgtcatc cccggcttaa tcaatatgca
     3241 tactcattcg gcaatgtcac tgctgcgtgg tgtatccgaa gatgttccac taactgagtg
     3301 gctaaataag atttggtcta tagaaaaaca ccttcaaaaa gaatatgtct actggggagc
     3361 aaggcttgca attctcgaaa tgataaaaac cggtaccact tgttttctgg atatgtattg
     3421 gatgtcccct caggtggctc aggcagtaga ggagatgggt atacgaggag ttttgaccta
     3481 tgttttcctc gacaattatg atacggagaa ggctgagaaa cagaaatccg aatgtattcg
     3541 tattcatggc tattctaaac agtggcacaa acgatgcaaa ttcggagtct cgatccacgc
     3601 cgattacacc gtctctcctc atacgatggt atgggcttca gggtttgcta aagaaaaagg
     3661 tctgttaatc aatacacaca tatcagagac tgagaaggag gtgcttgccg acaaggagaa
     3721 acatggtaag actccagtgg ctttttacga ctctctgggc atccttgatt ccaatgtaat
     3781 tgccgctcat gcgctatggc tcgaggatga ggacattgag atttttgcta aacgaggcgt
     3841 tacggcagtc cataacgtca actccaactt gaagctcggt tccggctgta ggttcagata
     3901 cggtgactta aagtctgcgg gagtcaacgt ttgtcttggt accgatggag cagcttcttc
     3961 aaacaatctc gacatgcgtg aaagcatgaa gaccatgtcc cttctacaaa aaggttggac
     4021 gggagacacc acaattctgc ctcttaatga gcttatggat gtggcaactg tcaacggcgc
     4081 ccgtgctttg gatttagata cgggcagaat cgaggaggga gcactcgctg atctggtcct
     4141 ggtggagact cgttcagaag catttatccc gaatatcaat tttgaaagca atttcatcta
     4201 ctcagccaac agctcttgta ttgacacagt aatttgtgat ggcaatatcc tgatggaagg
     4261 gggcaaagtg gccggagagg agcaattatt ggaaaaagct gatgaaatgg cttggaaact
     4321 cataaacgca acatgatgga tcaaagaatt aaagcaattt acgaagctgc cgactatatt
     4381 cgtgagcgta caggtggatt tgtccccaag gtgggcatca ttctaggcag cggccttggc
     4441 aaacttgccg attcaataga gaatccgata actatccctt acgctgaaat accgcgtttt
     4501 tcacgctcca cggcgatagg tcataaaggc aacctgattc ttggcaccat tgccgggaag
     4561 cctgtatgcg ccatgcaggg aaggtttcac tattatgaag gttatcccat ggatcaggtg
     4621 accttacccg tcagagttat gaaagtactc gggatcgagt atctctttgt ctcgaatgca
     4681 gcaggaggag tcaatttgga ttataaagtg ggtgacttga tgattataag cgatcatatc
     4741 aatcgtcttc ctaatcctct cgtgggatcc aatctcgacc aattcgggcc acgtttccct
     4801 gacatgacgc gtccttacga cctcaatctt atccgcaaag cggaaaatat cgccgaaaag
     4861 gaaggtataa aactcttcaa gggtgtctat ttggccggta ccgggccatc ttacgagact
     4921 cctgccgagt accgtttttt ccgtatgatt ggggcggatg ccatagggat gtcaaccatt
     4981 ccggaggtca tagtagcccg acactcggat atccccgtat tcggcatctc tgtcatcacc
     5041 aatgaggccc atggcgattt tgccgaggat tttgtaaatg atggtgatga tgtggtaaag
     5101 gcggcagatg cagctgcggg taccatgaca tatcttttca gtaaactaat aggatcacta
     5161 taatgttgac aacagttttt ccatacgatt cagtggagtt gagttccaat ggtcgtgaag
     5221 tgataatact tgaccagtct ttgcttccca atcaggagaa atttcttcac ttgactacgg
     5281 cagagcaggt cttttttgcc atcacgctta tgaaggtgcg cggagcgcct gccataggca
     5341 ttgccgcggc ttttggtttg gccatgtcaa tcaacaggtc aaaatgtagg gaacttgcca
     5401 ccttcgaaaa ggaatttcta cgtacaaagc gctacctcta tatatcacgc cctacggccg
     5461 taaatctcat gtgggcgttg gaccgcatgg aggattgctt ttacaaagtc aaaaatagta
     5521 tagacgacac tgaccaaacg gtaatagaca tcataaaaaa cgcccttatt gctgaagcgc
     5581 gctctataaa ggttgaagag accagaatgt cggtagctat tgcagagcat ggtgtctcat
     5641 tgcttaccca tggctgcggt gttctaactc actgtaatgc cggtcacctt gctacgtccc
     5701 gctatggcac ggctttgagt cctatttatc ttgctcacca gaagggctat tctccaatgg
     5761 tctacgtaga tgagaccaga ccattattgc agggagctcg tctgactgcc tatgaactga
     5821 tgaaagcagg gatagacgtt actcttattt gtgataacat gtcctcgata gttatgagtc
     5881 aaaagagggt ggactccgtt tttgtcgg
//
LOCUS       DAOSIE010000072         5907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_12176_length_5907_cov_2.938483, whole genome shotgun sequence.
ACCESSION   DAOSIE010000072 DAOSIE010000000
VERSION     DAOSIE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12176_length_5907_cov_2.938483"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="PLI69_03320"
     CDS             <1..97
                     /locus_tag="PLI69_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOG41552.1"
                     /translation="MTFSHQMVRLIFVEQLYRAFTIIKGEPYHHK"
     gene            94..3705
                     /locus_tag="PLI69_03325"
     CDS             94..3705
                     /locus_tag="PLI69_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG41553.1"
                     /translation="MKGVNSIYNRITGAANKYLTIFMAAIAIVFAVISFVGSTGTYNL
                     RREVRKVQKQVHHRQYIIEKYAQRAINTPDDQWVDLTDLPDDMVIYKYVSDTLQSWAN
                     QFPISNDEVDAYSFTYRLHYLSNDNLFSSPLAYLGLEEQYVNLGSAWYIVNTYVSPSY
                     RTKVITGILVKTEYLDDKGLKNYSNKKLRIGAGFDPVSVNVDAAGVVYGIEGLPLFSL
                     VSESPSLVRSTNLTPMWISMLFAVIALFLFHLRKRSWLSLMITAFFLFAIRVVAFVVV
                     ARNDMSGEIFSPIYYADNRLFDSLGNFLINNVLIALLTYAVFITRLPIVKRIKRSGST
                     MRSVGAIVAVVTTILLALYIHLTFRSLIINSNIVLEPFRIDELSWYSILAYFSSAMIF
                     LGLLLMLQLLVLIFRRRCTFSLLSWFNMTLYVLVISLYCVTMIVIYGFEKEYETNRVR
                     TNKLAMERDLSLELQLRNVEETIAHDPIIALLTTVDRREVIRSRLLERYLYRNTAQKY
                     NIELTVCSPNDLLDLGHGSELVLCYPFYQDQITRFGTPLAPNSRFFYINKYNGLTSYL
                     GLFTFFDPVSSRVTRLFVEIESKYSSELVGLPTILSGDEGYNDLRLPRHYSFARYSNG
                     RIITYKGDYEYPVIFDKTIPDGYSMQIKKGYVHFINKFSADEITLVSRPSQRFFSNVV
                     SFSYIAIFFGLFFSIMTFWGRGSKLFSLPKKSIKRKITIIITSAMIFSILFLGVASIH
                     YISRLNKTGNEREMDEKITTARSVLSEYCKYANVYNQVQSLEFYNEMDELSKVIRTDI
                     NLYDTHGGLIRTTQPDIYEQFLMGKRMNANAYHEISHNNALKYTTMEEVAGMKYFSVY
                     APLFNNQGAMVAIINLPYFSRSADMLSASSSMIATIINIYLILIILAFIISVLLSNSL
                     SRPLVEIKERIQGLTTSSNRNRYISYHNNRDEIGVLVSAYNKMVDDLDHSRQQLAQAE
                     REQAWKEMARQIAHEIKNPLTPMKLRIQYLIRMKDEGRSGWEEQVEPTLNSILGQIAN
                     LSEVADAFSSFSRFYNEEVVDVNLNAAIAEQVFFFDTNDDILIEIRNNVPNPVVSARR
                     QQLSRVFVNILQNAIESIQNARGRGKILVTINAVEREEGVFYRVDFEDDGLGVSEENL
                     ERLFHPNFTTKSGGTGLGLAICRNIIEQSQGSISYSKSSLGGACFTILLPAKKPTSF"
     gene            complement(3692..5002)
                     /gene="der"
                     /locus_tag="PLI69_03330"
     CDS             complement(3692..5002)
                     /gene="der"
                     /locus_tag="PLI69_03330"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOG41554.1"
                     /translation="MNLVAIVGRPNVGKSTLFNRLVGMRQAIVDETAGVTRDRHYGTC
                     EWCGIEFSVVDTGGFTSKSDDIFEEEIRKQVMIAIEECDLVLFVVEVGTGITDFDAEI
                     ADLLRRSNKPVVLAVNKVDTGEKMYGAYEFHALGLGDPWSISSANGSGTGDLLDRIVE
                     LLPADANIKDDPYANVPRIAIVGKPNVGKSSLVNAFLGEERNIVTPIAGTTRDSISTY
                     YNKFGHEFILIDTAGLRKKTRVKEDLEFYSVMRSIRAIENSDVCILMVDASTGIEAQD
                     MAIFNLIARNKKGCVIVVNKWDTVEKGNNTMKEFTEGIKKRIAPFSDVPIIFTSVINK
                     QRILDVLNAAATVYSNYSRRIPTSELNEVMLPEIENYPPPAWKGKYIKIKYVTQLPTK
                     SPSFAFFCNLPQYVREPYKRFLENRLREHFDFSGCTIQIFLRKK"
     gene            complement(5016..>5907)
                     /locus_tag="PLI69_03335"
     CDS             complement(5016..>5907)
                     /locus_tag="PLI69_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG41555.1"
                     /translation="IKKGAFDFIEKPLDLNRILITLKNATDKTQLVKETTILKKKVNA
                     KYEIIGESAAIKHIKDIVDRVAPTEARVLITGSNGTGKELVARRLHEKSTRASAPFIE
                     VNCAAIPSELIESELFGHEKGAFTSAVRQHKGKFEQADGGTLFLDEIGDMSLAAQAKV
                     LRVLQEHKITRVGSDKDITVNVRVVAATNKNITEEIEKGNFREDLYHRLSVILIHVPP
                     LSERIEDIPLLVEHFIKQISRDMGIAAKKVTPEAYDELSKLPWRGNIRELRNVTERLM
                     ILCGPTITKEDIIAYATPAI"
BASE COUNT         1540 a         1323 c         1272 g         1772 t
ORIGIN      
        1 gatgacattt tcccatcaaa tggtgcgcct gatttttgtc gaacaattgt acagggcatt
       61 tacaataatt aaaggtgagc cataccacca caagtgaaag gagtcaatag catatataat
      121 cggattaccg gagctgccaa caaatatttg acgatattca tggcggctat tgccattgtt
      181 tttgccgtta tctctttcgt tggcagtaca ggtacctata atcttcgccg tgaagtacga
      241 aaggtgcaaa aacaggttca tcatcgacag tacatcatcg agaagtacgc gcaacgtgcc
      301 atcaatactc cggatgacca gtgggtggat cttactgacc tgccggatga tatggtgata
      361 tataaatatg tctccgacac ccttcaatcc tgggccaatc agttccccat aagtaatgat
      421 gaagtagatg cctattcttt tacataccgc ctccattatc tgagtaatga caaccttttc
      481 agctcgccgc tggcttattt gggactcgag gagcagtatg tcaatctagg ctcggcatgg
      541 tacattgtca acacctatgt ttctccttca tatcgaacca aagtcataac cggtattttg
      601 gtaaagaccg aatatcttga tgacaagggt ctgaaaaact actcaaataa gaaacttcgc
      661 attggtgcag gttttgatcc tgtaagcgtc aatgtggatg ccgcaggtgt agtatatggt
      721 attgaggggc tgccactctt ctcactggtt tcagagagcc caagtcttgt caggagtaca
      781 aatctcaccc cgatgtggat ttcgatgctt tttgcagtga ttgctctctt tctattccat
      841 ttaagaaagc gtagctggtt atcccttatg ataactgctt ttttcctttt tgcaatcaga
      901 gttgtggctt ttgtcgtggt agcacgcaat gatatgtcgg gagagatatt ttcgcccatc
      961 tattatgcgg acaaccgtct ttttgattct ctgggcaatt ttttgattaa taatgtctta
     1021 atagccctcc tgacctatgc tgtcttcata acccgtctcc ctatagtcaa gcgcataaag
     1081 agatccggca gtaccatgag gagtgtaggt gccatagttg cagttgtaac gactatcctt
     1141 ctggcacttt atatccattt gacattcaga tctctgatta ttaactccaa catagtcctg
     1201 gaacctttcc gtatagatga gctttcctgg tattccattt tggcctattt ctcttcggca
     1261 atgatatttc tagggcttct tctgatgttg cagctactgg tactcatttt ccgtcgaagg
     1321 tgtacattct cactcctgag ctggttcaat atgacactat atgtattggt aatttcgctc
     1381 tactgcgtga caatgattgt gatatacggt tttgaaaaag agtatgagac aaaccgtgta
     1441 cggaccaata aacttgctat ggaaagagac ctgagtctcg aactccaatt gcgtaatgta
     1501 gaggagacca tagcccacga ccctataata gcactgctga caactgttga cagacgggag
     1561 gtgatccgca gccgtttgct tgaacgctat ttatatcgca atactgctca gaaatataat
     1621 atagagctga ccgtttgctc tccaaatgac ctgctcgatt tagggcatgg cagtgagctg
     1681 gtgctctgct atcctttcta tcaagaccaa ataacaaggt ttggtacacc tttggcccct
     1741 aattcgagat tcttctatat aaacaagtac aatggtttga cctcttacct gggcctattc
     1801 acattttttg accctgtaag ttcgagagtc acccgactat ttgtggaaat tgagtccaaa
     1861 tactcttcag agttagtagg gctaccgacc atattgagtg gagacgaggg ttataatgat
     1921 ctccgacttc ctcgtcatta ctctttcgca agatatagca atggccgtat tattacctac
     1981 aagggggatt atgagtatcc tgtaatcttt gataagacta tacccgatgg ttattccatg
     2041 caaattaaaa agggttacgt acactttatc aataagtttt cagctgatga aatcacactt
     2101 gtgagccgac ccagtcagag gtttttctca aatgtggtct cattctccta tattgcaatt
     2161 ttcttcggac tcttcttctc cataatgact ttctggggta ggggcagtaa acttttctcc
     2221 ctccctaaaa aatccatcaa gagaaagatt accatcatca ttacctctgc aatgatcttt
     2281 tccattctct tccttggagt agccagcatc cactatataa gcaggctcaa caaaaccggt
     2341 aatgagcgtg agatggatga gaaaatcacc acagcccgca gcgttctttc agagtattgc
     2401 aaatatgcaa atgtttacaa tcaagtacag tcgctggagt tttacaacga gatggatgag
     2461 ctctccaaag tgattaggac cgacatcaac ctttatgata ctcatggtgg gctgatccgt
     2521 accacacaac ccgatatcta cgaacagttc cttatgggaa aacgtatgaa tgccaatgca
     2581 tatcacgaga tttcacacaa caatgcccta aaatatacta ccatggagga ggtggcaggg
     2641 atgaagtact tttcggtcta cgctccgctc ttcaataacc agggtgctat ggtagctata
     2701 atcaaccttc cttacttctc tcgttccgct gatatgctga gtgcttcatc ttcgatgatt
     2761 gctaccatca ttaacatcta cttgatcctg atcatcctag catttataat aagtgtactg
     2821 ctctccaatt ctctctcgcg gcctctggta gagattaaag agaggataca gggacttacc
     2881 acctcctcca ataggaacag atatatttca taccacaaca acagagatga aataggtgtg
     2941 cttgtgagcg cttacaacaa gatggtggac gacctcgacc atagtaggca gcaactggca
     3001 caggctgagc gcgaacaggc ctggaaggag atggcacgtc agattgccca tgagataaag
     3061 aatcctttaa cccccatgaa acttcggata caatatctga ttagaatgaa ggatgaggga
     3121 cgctcaggat gggaagagca ggtagaaccc actctcaatt ccattttggg gcagatagcc
     3181 aatcttagtg aagtggcaga tgcattttcg tctttctcca gattctacaa tgaagaggtg
     3241 gtggatgtga atctcaatgc tgctattgca gagcaggtat ttttctttga taccaatgat
     3301 gatattttaa tcgaaattcg caataatgtt cccaatcctg tggtttcggc gaggagacaa
     3361 caactatccc gtgtctttgt aaacatcctc cagaatgcta ttgagtcaat tcaaaatgca
     3421 cgggggagag ggaagatcct tgtgaccata aatgcagttg agcgtgaaga aggagtcttc
     3481 tacagagtgg attttgaaga tgatggcttg ggagtctccg aagaaaatct ggagagactt
     3541 ttccatccta attttaccac aaaaagcgga ggtaccggtc ttggacttgc tatatgtcgt
     3601 aatattatag agcaatcaca gggttcaata tcctactcaa aatcctcttt gggtggtgcc
     3661 tgctttacaa tccttcttcc tgctaaaaag cctacttctt tctgaggaaa atctgtatcg
     3721 tgcagccgct gaaatcgaaa tgctctctga gtctgttttc gaggaatctc ttataaggct
     3781 cccttacata ttgaggtaaa ttgcagaaaa atgcaaaaga aggtgacttc gtgggaagct
     3841 gggtcacata tttgatcttt atatatttgc ctttccaggc aggcggaggg tagttttcaa
     3901 tctcgggaag cattacttcg ttgagttcgg aagtagggat cctcctggaa taattggaat
     3961 agaccgtggc agcggcatta agtacatcca ggatcctctg tttatttatt acggaagtga
     4021 atatgattgg aacatcactg aatggggcaa tgcgtttttt tatcccttcc gtaaactctt
     4081 tcatcgtatt attacccttt tcgacagtat cccatttgtt gacaactatc acgcaccctt
     4141 tcttgttgcg ggcaataagg ttgaaaatag ccatatcctg tgcttctatt ccggttgagg
     4201 catcaaccat cagtatacat acatcggaat tttcaatggc tctgatggaa cgcattacag
     4261 aatagaactc caagtcctct ttaacacggg ttttcttacg aagaccggct gtgtctatga
     4321 gaatgaactc gtgtccgaat ttattgtaat aggtagaaat tgaatctcgc gtggtgccgg
     4381 caataggcgt cactatattg cgttcttccc ctaaaaaggc attgacaagt gaagatttgc
     4441 ccacattggg cttccctact atagctatgc gtggaacatt ggcgtaggga tcatccttaa
     4501 tattcgcatc tgcaggaaga agttccacaa tcctatccag caggtcacct gtaccactgc
     4561 cgttggcaga agagatagac caggggtcgc ccaatccaag ggcgtggaat tcgtaggcgc
     4621 catacatctt ttcacccgtg tcgaccttgt tcactgcaag aactacaggt ttattgctcc
     4681 tgcggagcag gtctgcaatt tcagcatcaa agtcggtaat tccggtacct acctccacga
     4741 cgaatagcac aaggtcacat tcttcgatgg caatcatcac ctgcttgcgg atttcctctt
     4801 caaatatgtc atcggatttg gaagtaaatc ctcctgtatc caccaccgaa aactcgatac
     4861 cgcaccattc acaagtacca tagtggcggt cgcgtgttac accggcggtc tcatcgacta
     4921 ttgcctgcct catacctacc aggcggttga aaagggtcga tttccctaca ttcggtcttc
     4981 ctactattgc taccagattc atattgaaat gtgtgttata tcgccggagt agcgtatgct
     5041 attatatctt ccttggttat tgtcggtccg cagaggatca tcagtctttc ggtgacattg
     5101 cgaagttcac gtatattacc cctccatggg agcttactca gctcatcata ggcctcggga
     5161 gtgacttttt tggcagctat gcccatatct ctactgatct gttttataaa atgttccacc
     5221 aggagcggaa tatcttcaat gcgttcggaa agaggaggaa cgtggatcag gattacgctc
     5281 aggcggtggt acaggtcttc tctaaaattg cccttttcaa tctcctcggt tatgtttttg
     5341 ttggttgcag ccactactct gacatttaca gtaatatcct tatcactgcc tactctggtt
     5401 attttgtgtt cctgaagtac ccttagcacc ttggcttgtg cagcaagact catatctcca
     5461 atttcgtcaa ggaagagagt gccgccatcc gcctgttcaa acttaccctt gtgttgtctt
     5521 accgcggagg taaaagctcc cttttcgtgt ccgaaaagtt cgctctctat cagctcactc
     5581 ggtatggctg cacagttgac ttcgataaag ggtgcagagg cgcgggtgct tttttcgtga
     5641 aggcgtctgg ccacaagttc cttgcctgta ccattggatc cggtaatgag gactcttgcc
     5701 tcagtaggag ccactctgtc tacaatatcc ttgatatgct tgatggctgc cgactcgcct
     5761 ataatttcat attttgcatt tactttcttt ttgagaatag tggtttcctt taccagctga
     5821 gtcttgtcag tagcattctt gagggtgatc aggattctgt tgaggtcgag tggtttttct
     5881 atgaagtcga atgctccctt ttttatg
//
LOCUS       DAOSIE010000073         5892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_12214_length_5892_cov_4.536406, whole genome shotgun sequence.
ACCESSION   DAOSIE010000073 DAOSIE010000000
VERSION     DAOSIE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12214_length_5892_cov_4.536406"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLI69_03340"
     CDS             <1..296
                     /locus_tag="PLI69_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41556.1"
                     /translation="AGCGKIVPVKISELYKNTHKYENETVLVKGKVNKSISMFGLNGF
                     ILTDGTKELMVVGHEATPAPGEKITVRGTLSVPLRWQDDALLVLRASPEDEED"
     gene            504..1019
                     /locus_tag="PLI69_03345"
     CDS             504..1019
                     /locus_tag="PLI69_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOG41557.1"
                     /translation="MKTKIEIIQADITTIKVDAIVNAANNSLLGGGGVDGAIHRAAGP
                     ELLAECRTLNGCETGKAKITKGYNLPAKHVIHTVGPIWRGGNNNEAEKLKSCYKSCFK
                     LALENNVKSIAFPSISTGAYGYPIKPASQIAIQEMLTFLKENPDFDKIIVACFGDNVY
                     NCYIKALSEND"
     gene            1709..2143
                     /locus_tag="PLI69_03350"
     CDS             1709..2143
                     /locus_tag="PLI69_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOG41558.1"
                     /translation="MKLKSCISENPHQNLKSVAFEPKFLLKSVVVTCTPNKRALFENM
                     IVSEYMKRKYHNNERQMDLWYWRDSEKNEVDLLIEKPQETEIIEIKATQTIMSDLFKG
                     LNHYAALETEVKLTKTLVYGGNESQKRSVARVLSWNDVNVSV"
     gene            complement(2568..3587)
                     /locus_tag="PLI69_03355"
     CDS             complement(2568..3587)
                     /locus_tag="PLI69_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOG41559.1"
                     /translation="MEIKYRTLWIWLLAVLLLGMGASCEKEEEKEEEKLYMSGFVDYY
                     IPTFVGIGETITLTASGITEPSKVDYKWYFGASYNDTLSSRTVTVTFPDSLGTFAIIG
                     IAVAPGYYNSSATQHVTTVDIRHEKSLKGLKYSNKVFIDNRDGIEYEYVTIGNLDWFV
                     RNLAYYKKGSAFRHSPITHTLFGRFYSWEEATGGQSASGLGGGPQGVCPKGWSVPTNE
                     DWVDLATAVAGKQMTFVDNWDGLGDKLSVDAWFNGERIWPYSPDNIHSNSAGWNAIPM
                     GNTQYDHSLFKGFNEYGFWWSSTQKNDAQAYYRYIHSESSFFPMNFTSKEHFGASVRC
                     VRLAK"
     gene            3707..4747
                     /locus_tag="PLI69_03360"
     CDS             3707..4747
                     /locus_tag="PLI69_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOG41560.1"
                     /translation="MINKSTIIPLLIVTLLIAGCSTGKGRYERIEGYAQGSTFHIIYS
                     RSTGAGIKSAQVEERVNSLLAAIDNSLSGYNKGSLLSRFNAGEEIIPDSLFIEVFNRS
                     KEIWLESSGAFDPSAAPLFDLWGFGFSNRDTVTQHLIDSALLLVGMDMVSIEGSGDSA
                     RVTRELPGVKLNFNAIAQGYTCDVIARALDDFGCADYLVEVGREIVCKGLSSRGDKWK
                     VGLDKPLDGNFDEGANLQEIISMSDGALVTSGNYRKFYIENGEKRSHTIDPATGRPVK
                     HNLLSATVIASDGATADAYATWMMVIGEKRAMELASQRNDIEVYLVYGEQDSMKVWHT
                     EGFPIYLNESKK"
     gene            4829..>5892
                     /locus_tag="PLI69_03365"
     CDS             4829..>5892
                     /locus_tag="PLI69_03365"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG41561.1"
                     /translation="MERRDFIRTTLIGAAALGIGFYGGRKIGVRHPFETIIKNGVIYT
                     GDGKAPIKGDIGIRDGRIAAIGTLGDNADIIIDAKGVAISPGWIDIHTHTDTNLFLAP
                     KGDSRIFQGITTDIGGNCGDSPFPYSDAHYEANKETLRHGYPFWQDIDGFYDAYRKQG
                     IGINYGSLVGQGQLRSAVIGDNNIPATPETLAKMCSILDREMEMGALGISLGLEYAPS
                     SYASDEELIELCKVVAKHDGLYAIHMRNEDDFVEESLRDAIRIAKESGARLQVSHLKA
                     QNAANWHKMPALLAMIDQAKAEGIDIAFDRYPYIAFSTGLSSFIPINDRQGSSEEVLA
                     RLADDVKAAEIGRYAESRIKR"
BASE COUNT         1730 a         1247 c         1319 g         1596 t
ORIGIN      
        1 ccgcaggttg cggtaagatt gtccccgtta aaatatccga actttacaag aatacgcaca
       61 aatatgagaa tgagacagta ctggtaaaag gtaaagtcaa taaaagtatc tccatgtttg
      121 ggcttaacgg ttttattcta accgatggca ctaaagaatt gatggtggtt ggccatgagg
      181 caacccctgc tcctggtgaa aagataaccg ttcgcggtac gctctcagtg ccacttagat
      241 ggcaagacga tgcgttgttg gtgttacggg cctcgccgga ggatgaggag gattaaagga
      301 gaatcagaat ccttgcccat tacatacaaa ttatattgtc acttaacaga agggagcgat
      361 ttagattaaa caaactgtct cgttccagac attaagttca tcttgcttgt aataagataa
      421 actaattgtt ttatatttcg aggagaatgt aatagtatgt gcgtttctga agctgtataa
      481 tcgaataata tatcatcatc caaatgaaaa ccaaaataga aataatccaa gccgatatca
      541 cgacaataaa ggttgatgct atcgtgaatg ctgccaataa ctctttatta ggtggtgggg
      601 gagtggatgg ggcgatccat agagcagcag gccctgagtt gcttgcggag tgcaggaccc
      661 tgaatggctg tgagaccggc aaggctaaaa tcacaaaagg ttacaatttg cctgcaaagc
      721 atgtgatcca tactgtcggc ccgatttggc gcggaggaaa taacaatgag gctgaaaagt
      781 tgaaaagttg ctataaatcc tgttttaaat tggctcttga gaacaacgtc aagtcaatcg
      841 catttccctc tataagtaca ggagcctacg gatacccaat aaagcctgcc tctcaaatcg
      901 ccatacagga aatgttgact ttcttaaaag aaaaccccga tttcgataaa atcatcgtgg
      961 cctgtttcgg tgacaatgtt tataattgtt atatcaaagc gctatccgaa aacgactgaa
     1021 ataatgacca gtaacttatc cctgacgaac ttcatggcat tctgaaattg tatttcatta
     1081 aaaggagata ggggcttcca tttctgcttg atagtcatcc aattgtacct tactttataa
     1141 aaacttcgta aaattgtaag ttttgatcaa ggatacttgc ctttgtgatt tttggaaagt
     1201 gtactttttg ccccaaattt ctattgaaaa gtgtagttcc ggactcaaaa tccatgcaaa
     1261 tcgaaagttg tactttagaa atacatgtaa aaacaaagta gaacttttga tttacataga
     1321 caattctttg ctatacaccc tcggttctcc ctttgattgt gacaacaggt cttacctaaa
     1381 gattaagata tttgcgtatg agttaggcaa aaacatcaca aatttgccaa agttttacgg
     1441 tttttttgtt gtgattattg tgaaaaatta cgctttttgg gaaggatttc cgaaataaat
     1501 aaattaatta gcgaaatatt ctataatttg ccattagtcg ttttttactc ttatttcatt
     1561 gatactctag tatttaacga caaaagtaca gttaaataat catatcatag gtatatcata
     1621 ggttcttaaa tagttcattt tcaaatagtt ggcagcgact tgctttttat gttttgcgat
     1681 ttttaaattt tattcaaatc caaaatacat gaaattaaaa agttgtattt cggaaaatcc
     1741 tcatcaaaat ttgaaaagtg tagctttcga acccaaattt ctcttgaaaa gtgtagttgt
     1801 gacttgtacc ccaaataaaa gagcactatt tgagaacatg atagtttctg agtatatgaa
     1861 acggaagtat cacaataatg agaggcaaat ggacttgtgg tattggcgtg actcggaaaa
     1921 gaatgaggtg gatttgctta tcgaaaagcc gcaggaaacc gaaattatag agataaaagc
     1981 aacacaaacc attatgtccg atttgtttaa gggattaaac cattatgctg cgctcgaaac
     2041 ggaagttaaa ctgactaaga cattagttta tggaggcaac gaaagtcaga aacgcagcgt
     2101 agccagggtc ttgtcttgga atgatgtgaa tgtgtcagta taacttaggc ataaagattc
     2161 agatttgcca aactgataag agtttatcac acaaaaaaaa gccctctcac ctcatgtgca
     2221 agggcttttt agttcttaaa gccggcctta taattgtgca acccgacaat aaaattattc
     2281 taccaattta tgatctcccc atttgctttt acggcaaatg caactccgtg aacagattgt
     2341 tccacatcat taatagccct catggccttg tcagaaatct gaaatcttcg tagcttataa
     2401 gtggattggc ttttgtctta gccgtggttt catcaattcg atatgaaaaa tcctaaaatg
     2461 ttgaatagga gtcaagcaag tatagttcga ctttcccgtc tgaagatttt ttttcgcaac
     2521 ccaaaactca gaactcagaa ctcagaactc gaaaccaaca cttctcacta tttcgctaat
     2581 ctcacgcagc ggaccgatgc tccgaaatgt tccttagagg tgaaattcat tgggaagaag
     2641 gagctttcgc tatgaatata gcggtagtag gcctgagcat catttttctg ggtggagctc
     2701 caccagaacc catactcatt aaaacccttg aaaagggagt ggtcatactg agtattgccc
     2761 atcgggatgg cattccatcc ggcactattg gagtgaatat tgtcgggaga gtagggccag
     2821 atcctttcgc cgttaaacca tgcatccaca gagagcttat cgccaagtcc atcccagtta
     2881 tccacgaagg tcatctgttt tccggcaact gcggtggcca gatctaccca atcttcattg
     2941 gtgggtacag accatccttt tggacagact ccctgtggcc cgccgcccag tccggaagcg
     3001 ctctggccac cggtggcctc ttcccaagaa tagaagcgtc cgaagagggt atgagtaatt
     3061 ggcgagtgcc ggaaagccga gccctttttg tagtatgcga ggttgcgtac gaaccagtca
     3121 agattgccta tggtaacata ctcgtattct ataccgtcgc ggttgtcaat aaaaacctta
     3181 ttactatact taaggccctt tagggatttt tcatgtctga tgtccacagt agtaacatgt
     3241 tgtgtggctg atgagttgta gtagcccgga gctactgcta tgccgataat ggcgaaggta
     3301 ccgagactat cgggaaatgt aacagtcact gtccttgagg agagagtgtc attgtatgaa
     3361 gctccgaaat accatttata atcgaccttt gaaggctctg taataccgga ggccgtaagg
     3421 gtaatcgtct cgccgatgcc gacaaaagtg ggaatgtaat aatctacaaa tccgctcata
     3481 tagagctttt cttcctcttt ctcttcttcc ttttcacaag aagcgcccat gcccaacagg
     3541 agaactgcca gcaaccaaat ccaaagagtt ctgtatttaa tttccattgt ctgttatttc
     3601 caacctacaa atatgagtat tttttggcgc aatagcaatt tttgattttt tatagtaaat
     3661 ttgcaccaac tcttttgcaa tttctccaaa atcgtgccac cctccaatga tcaacaaaag
     3721 cacaataata ccgttactga ttgtaactct tctcatagcc ggatgttcca ccggcaaggg
     3781 cagatatgag cgcattgaag gttatgctca ggggagcaca tttcatataa tatattcccg
     3841 ttcgacaggt gcagggatta agagtgcgca ggtggaagag agggtcaata gccttctcgc
     3901 cgccatagat aattccctct ccggatataa caaaggttcg ctcctttccc gtttcaatgc
     3961 gggagaagag attattcctg attcgctctt tattgaggta ttcaaccgct ccaaagagat
     4021 atggctggag agctccggag catttgatcc ctctgcagca cctctgttcg acctctgggg
     4081 cttcggtttc agtaaccgtg ataccgtcac tcaacacctc atagatagcg ctctgcttct
     4141 cgtgggtatg gatatggtct ccatcgaggg aagcggtgac tcagctaggg ttacaagaga
     4201 actgccggga gtgaaactca atttcaacgc cattgcgcag ggctatacct gcgatgtgat
     4261 agcgagggca cttgacgact tcggatgcgc cgattatctg gttgaggtgg gtagagagat
     4321 tgtatgtaag gggctcagtt cgcgtggtga caaatggaaa gtgggtctgg acaagccctt
     4381 ggatggaaat tttgatgagg gcgccaatct tcaggagatt atttcaatgt cggatggggc
     4441 attggtcacc agcggtaatt accgcaaatt ttatattgag aatggagaaa aacgctctca
     4501 caccatagat cctgctacag gcaggcccgt caagcacaat cttctcagcg ctacggtgat
     4561 tgcttcagat ggagccactg ctgacgctta cgctacctgg atgatggtaa taggggagaa
     4621 aagggctatg gagctcgctt cacagcgaaa tgatatcgaa gtctatctcg tttatggaga
     4681 gcaggattcg atgaaggtgt ggcatacgga gggatttccc atttatttaa atgaatccaa
     4741 aaaataaaac cattatactc gtttaaaaac gcttacaatc acttgaaaac atctacacaa
     4801 cagatacaaa gataaaataa cacacaatat ggaaagacga gactttatca gaaccacact
     4861 cataggggct gctgccctgg gtatcggctt ttacggaggc cgcaaaatcg gcgtcaggca
     4921 cccattcgag accattataa aaaatggagt gatctatacc ggtgacggca aagccccgat
     4981 caagggtgat ataggtattc gagacggccg cattgcagcc atcggaaccc tcggagacaa
     5041 tgccgacatt atcatcgacg ccaaaggagt tgcaatctct cccgggtgga ttgacattca
     5101 cacccatacc gacaccaatc tcttcctggc tccaaaggga gacagccgca tattccaggg
     5161 cattaccacc gatatcgggg gaaattgcgg tgactcgccc tttccctatt ccgatgctca
     5221 ctatgaagca aataaagaga ctctaaggca cggatatccc ttctggcagg atatagacgg
     5281 cttttatgat gcataccgca agcagggcat aggcatcaac tatggcagcc ttgtggggca
     5341 ggggcagctc cgttccgccg tgatcggcga taacaacata ccggcaacgc ccgaaacact
     5401 ggccaaaatg tgctcaatac ttgacagaga aatggagatg ggagctttgg gtatctcact
     5461 gggactcgaa tatgctccca gttcttatgc atccgatgaa gagctgatag aactttgcaa
     5521 ggtggtggca aaacacgatg gtctttatgc catccatatg cgcaacgaag atgattttgt
     5581 ggaggaatcc ctcagggatg ccatacgtat cgcaaaagag tcgggtgccc gattgcaggt
     5641 atctcacctt aaggcgcaga atgccgccaa ctggcacaaa atgcccgcac tgctcgcaat
     5701 gatcgatcag gcgaaggcag agggcatcga tatagctttc gaccgctatc catatatagc
     5761 attttccacc ggactctcct cctttattcc tatcaacgat agacaaggct cctcggagga
     5821 ggtgctggca cgtctggccg atgatgtaaa agccgctgaa atcggaagat atgcagagag
     5881 ccgcatcaag cg
//
LOCUS       DAOSIE010000074         5888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_12224_length_5888_cov_4.387794, whole genome shotgun sequence.
ACCESSION   DAOSIE010000074 DAOSIE010000000
VERSION     DAOSIE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12224_length_5888_cov_4.387794"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1065)
                     /locus_tag="PLI69_03370"
     CDS             complement(70..1065)
                     /locus_tag="PLI69_03370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG41562.1"
                     /translation="MLRYRLLLLTLAIVSMLSVISCAKEVGESNDQIQARVLDSWIRV
                     KHPGSEKTPSGLYIIDFSEGTGRNVTDSAYVFVKYTVKELSGNILATNDRALSEQLGQ
                     FAHSRHYGYNIWRVDQDALYTGVEEVLKRMRVGGRATVAFTPALATVSVSLYSLFSSL
                     ESTAPFIFELELVDCVNDIYTDYEEPMLKLYSEKYYGGLDTIKRGFYFAKIKEAPAAD
                     SIVADAQIKIRYIGHMLDGVVFDTNIQDTAKRYRIYDSKKSYEAMDITFKSNIVDFKK
                     SNSIIEGFAEAIMRMRYGEKAVAFFWSQVGYGAGGSSPSIPEYTPLCFEIEVKGK"
     gene            complement(1080..1559)
                     /locus_tag="PLI69_03375"
     CDS             complement(1080..1559)
                     /locus_tag="PLI69_03375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG41563.1"
                     /translation="MFRILGLILVAGLMLFATVSCQGEKLLKLTNQEEQIDSYIKKEF
                     PDNEVIRLEGANRVIITPGIGPKAAAGDSIWFFYEAYLFSGSPAGMFWRDSANVMLGS
                     SHLVEGLNRGIEGMSLGEHSIILFSAKYGFYDKNNGLVPSMSALMYNVLLTGIKKNN"
     gene            complement(1588..2625)
                     /locus_tag="PLI69_03380"
     CDS             complement(1588..2625)
                     /locus_tag="PLI69_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOG41564.1"
                     /translation="MKKRFDTTLLEEMLAASVRPLLIGHFNPDGDSVGALTAMSHYLI
                     SRGMSPSAVLPSPYPDYLAFLIGDEMPVTIHSQEDDSARELIASADLLLCFDFNRLSR
                     TEQLEDDILASNAPKVLIDHHPQPEVEHFDLVYSDTELSSSCELLFWILLSMSDVRDD
                     VSRLSLKCSESLYVGMMTDTNNFYNSVSASTFEMASMLTDAGVDKTKLQEAVYNNYSA
                     DRMRLMGHLLKNCLHVIEDCGVAYLLLSAEDKRLYNFAIGDSEGFVNLPLSIKGIRIS
                     ALFSELDNGLDKYIRVSLRSKGDLDINHFASLYCNGGGHTNAAGGRLYMPFEEVASYI
                     EKSFREYLKIG"
     gene            2760..3368
                     /locus_tag="PLI69_03385"
     CDS             2760..3368
                     /locus_tag="PLI69_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41565.1"
                     /translation="MKKLFTYFILPLVIVILAVLIVRSITEPLKFNNERDARQEVAIQ
                     RLKDLRELQSTFKAAFGYYAPVMDTLVDFYKNGKITIVRQIGSMDDSVAVAKKLVRRE
                     NIEIFVRDTLFTTRTDFCVDSLRYIPFSHGDTVIMSSVIKVVSGVNVPLFEAKMPYTS
                     LLKEMDRQMIINLIAEREDTERYPGLMVGSIENPNNNAGNWE"
     gene            3368..3832
                     /locus_tag="PLI69_03390"
     CDS             3368..3832
                     /locus_tag="PLI69_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOG41566.1"
                     /translation="MVGFTLVPSEFFSSEDAYRILSEVVPLKDTDIVQYIELPDYKAV
                     LVYARQSDNKSPSEPPIAELLRFAPSISAHNRLVVSLDKEFIYIVLAEGDKLLLANAY
                     PATDIVTAQYFIFAALREFQINPRVTTIYFHGEIPSALTEEMFRHFRYVEFV"
     gene            3850..4386
                     /locus_tag="PLI69_03395"
     CDS             3850..4386
                     /locus_tag="PLI69_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOG41567.1"
                     /translation="MRIIGGSLKGKTIIPPSGLKARPTTDFAKEGLFNVLTNEIEFEE
                     TSVLDLFAGTGSISFEFASRGCKEIVAVEMNPMQADFIRKTAGKLGLSSLIVVRHNVF
                     DFINICTRRFDIVFADPPYQLEGIDTIPDRVIEHNILADNGYLILEHPNELNFTGYPY
                     FVKEKRYGNVHFTFFKKV"
     gene            4463..4536
                     /locus_tag="PLI69_03400"
     tRNA            4463..4536
                     /locus_tag="PLI69_03400"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4497..4499,aa:Asp,seq:gtc)
     gene            complement(4676..5827)
                     /locus_tag="PLI69_03405"
     CDS             complement(4676..5827)
                     /locus_tag="PLI69_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41568.1"
                     /translation="MKKLSYLLVLIVVTFLSVTCSSNKRTLQRGDYYNATMGAIKTLR
                     SNPNSKKSIEVLQQAYPLAKENSMRKIQNALDVDVAGKYSIIADEYLVLNAIADAIYT
                     CPKAMDLFPRPEQYSKELREILPLAFEESYNQGERLLQLNTLIDAREAYYQFLKAERY
                     MPDYKDVREKIQVALYAATLKVVVQKPITPKRYQLSADFFYDNLMAQMSRITANQLVR
                     FYTYGEAKEEGLDKPDQYLVLSFEDFTVGNLRETTNNSEISRDSVLVGTTTVNGVKQN
                     VYGTVKAKLTVYTREVISEGILSAKIINAYNNKVEESRNFPGRFVWVNEWASYQGDER
                     ALTNKQLKMTETKPIMPPPEQDLFIEFTRPIFDQAVGFARNYYSSITLP"
BASE COUNT         1721 a         1270 c         1129 g         1768 t
ORIGIN      
        1 ataatgacaa caataaagtt taacaaaatg tgtcaacttt ttttaggacg agacaactac
       61 acactctact catttgccct ttacttctat ttcaaaacag agaggagtgt attccggaat
      121 tgagggagag cttccaccgg ctccatatcc tacctgggac cagaaaaaag ccacggcctt
      181 ctctccatat cgcatgcgca taatggcttc ggcaaagccc tcaattatac tgttcgattt
      241 tttaaaatct acgatattac tcttgaatgt gatatccatt gcctcgtatg atttcttgga
      301 atcatatatc ctgtaacgct tcgccgtatc ctgaatattg gtatcgaaaa caaccccgtc
      361 aagcatatga ccgatatagc gaatcttgat ttgcgcatct gctacaatgg aatctgcagc
      421 aggagcttct ttgattttag caaaatagaa tccccgcttt attgtgtcca gacctccgta
      481 atatttctca ctatacaact taagcatagg ctcctcatag tcagtataaa tgtcatttac
      541 acaatcgaca agttccagtt caaatataaa aggcgctgta ctttcaaggg aagaaaagag
      601 agaatataga gaaacagata cggtggcaag tgccggagta aatgctacag ttgccctacc
      661 tcctaccctc atccttttta aaacctcttc cacacctgta taaagcgcat cctgatcaac
      721 ccgccaaata ttgtagccgt aatgcctgga gtgagcaaac tgtcccaatt gctcggaaag
      781 ggcccgatca ttggtagcca gaatgttgcc tgaaagttct ttaacggtat atttaacaaa
      841 aacataagca ctatcggtca cattgcgacc ggtaccctca gaaaaatcaa tgatatatag
      901 tcctgaagga gtcttttcag agccgggatg tttaaccctt atccaagagt cgagcacacg
      961 tgcctggatc tgatcattac tctcgcctac ctcttttgca caagatatga cagagagcat
     1021 gctaactata gcaagagtta aaagcagtaa tctgtatctt aacatataac ctttaattgt
     1081 cagttgtttt tctttatacc tgtcagcaaa acattgtaca tcaatgcaga cattgagggt
     1141 actaatccat tgtttttgtc atagaaacca tattttgcag aaaagagaat gatggaatgt
     1201 tcgcccagag acatgccctc aataccacga ttgagtccct ccaccaaatg tgaagatcca
     1261 agcatcacat tggcactatc acgccagaac atacctgcgg gactgcctga gaagagatag
     1321 gcttcgtaaa aaaaccatat tgagtctcct gcagcagcct taggtccaat gcccggtgta
     1381 attataacac ggttggctcc ctccagacga atgacttcat tgtcgggaaa ttctttcttt
     1441 atgtagctgt caatctgctc ctcctgattg gtgagcttta gcaacttttc gccttggcag
     1501 gaaaccgttg cgaaaagcat taaaccagcc accaatataa gtcctaaaat ccgaaacatt
     1561 ttgcaaatat aatacaaaag atgtgcctca accaatttta aggtactctc taaaactttt
     1621 ttctatatat gaagcaacct cttcaaatgg catatagagt cttccgcccg ctgcattggt
     1681 atgaccgccg ccattgcaat agagagaagc gaagtgatta atatccaaat cccccttaga
     1741 gcgtagcgag acacgaatat atttatccag gccattatcc agctccgaga aaagagcact
     1801 aatcctaata cccttgatgg agagcggtaa attgacgaac ccctcagaat ctccgattgc
     1861 aaaattatat aatcgcttat cctcggcgct aaggagcaga taggcaacac cgcaatcctc
     1921 tatcacatgg agacaatttt ttagcagatg gcccataagt cgcattctat cggcggaata
     1981 gttattataa acagcctcct gaagcttggt tttatctact ccggcatcgg tcagcattga
     2041 agccatttca aaagtcgatg cgcttacgga gttgtagaaa ttgttggtat cggtcatcat
     2101 ccctacatat agcgactctg agcactttag gctgagtcgc gatacatcat cccgaacatc
     2161 gctcatactc aaaagtatcc agaaaagcaa ctcgcatgat gaagagagct ccgtatcgga
     2221 ataaacaagg tcaaaatgct ccacttcagg ttggggatga tggtctataa gcacctttgg
     2281 ggcattactg gccaatatat catcctccaa ctgttctgta cgacttaagc ggttgaaatc
     2341 gaaacagagt agcaaatctg cagatgcaat cagctctctt gcactgtcat cctcctgtga
     2401 gtgaattgtg accggcatct catctcctat gagaaaagca agataatcgg gataaggact
     2461 gggcaaaacc gccgaagggg acattcccct cgatatcagg taatgactca tggccgtgag
     2521 ggcacccacc gaatctccat ccggattgaa gtgaccgatc aagagaggcc taacggatgc
     2581 tgccagcatc tcctcaagaa gtgttgtatc aaatcttttt ttcactcttt ttctcttttt
     2641 atagttgcaa aattaaaaat atattgcaag tactaaatat tatttttact tttgtaagtc
     2701 aaaatagaac ttttttctga gactcacaaa ggacaatatt atttaattaa aattataaaa
     2761 tgaaaaaact attcacctat ttcatcctcc ctttagtgat tgttattctt gcagtactta
     2821 ttgttaggag tatcaccgag cctttgaagt tcaataatga aagagatgcc cgtcaggaag
     2881 tcgctatcca acgcctcaag gatctccgcg agcttcagag tacatttaaa gcagcttttg
     2941 gctattatgc accggtaatg gacacactcg ttgattttta taagaacggt aagattacta
     3001 ttgtaaggca aattggttcg atggacgact ctgttgccgt ggcaaagaag ctcgtaagac
     3061 gtgaaaacat tgaaatcttt gtaagggata ccctttttac cacacgtacc gatttctgcg
     3121 ttgactcatt gaggtacatc cctttctctc acggtgacac cgttattatg agctccgtca
     3181 ttaaggtagt atcgggtgtg aacgttcctc tgtttgaggc taaaatgccg tacacctccc
     3241 ttctcaagga gatggatcgt cagatgatta tcaacctcat tgctgaaaga gaggacaccg
     3301 agcgttatcc cggcctaatg gtaggcagca ttgagaaccc caataacaac gcgggtaact
     3361 gggagtagtg gtaggattta ctctggtacc ttccgaattc ttttcatcgg aagatgcata
     3421 cagaatctta tccgaagtag taccgctcaa ggatacggat atagtgcaat acatagaact
     3481 gcctgattac aaggcagttc ttgtttatgc tcggcaaagt gacaacaaaa gcccttcgga
     3541 gccacctata gctgagcttt tgcgctttgc gccctcaatt tccgcacata accgacttgt
     3601 agtctcacta gataaagagt ttatatacat cgtattggcg gagggagaca aactgcttct
     3661 tgctaatgcc tatcctgcaa ctgacattgt cactgcccaa tattttattt tcgccgctct
     3721 gcgagagttc cagatcaatc cgagggtgac tacaatctat tttcacggtg aaatcccttc
     3781 agctctgact gaggagatgt tcagacattt ccgttatgtt gagtttgtct aaacaaatcg
     3841 gttgatctta tgcgtataat cggaggttca ctcaaaggca aaaccataat tccaccttca
     3901 ggtttaaagg cacgtcctac gactgatttt gcaaaagagg gcttgttcaa tgttcttaca
     3961 aatgagattg agtttgaaga aacatctgtc ctcgatcttt ttgccggtac agggagcatc
     4021 tcatttgaat ttgcatcacg agggtgtaaa gagatagttg ctgtcgagat gaatcccatg
     4081 caggctgatt tcatacgaaa gacagcaggg aaattggggc tttcttccct aattgttgtg
     4141 cgtcacaatg tttttgattt cattaatatc tgtaccagga gatttgacat agtatttgcc
     4201 gatcctccct atcagcttga gggtattgat actatcccgg acagggttat tgagcataac
     4261 atattggcag ataacggcta tctcatactt gagcatccaa atgaattgaa ctttacggga
     4321 tacccctact ttgtcaaaga aaaacgctac ggcaatgtac actttacttt tttcaaaaaa
     4381 gtatgattta tttttgccgg tttagaaaaa gtatctatat ttgcagttcc taacgggaaa
     4441 agttaaatac ttactactca aaggtgcggt agttcagttg gttagaatat cggcctgtca
     4501 cgccggaggt cgcgggttcg agtcccgtcc gcaccgcaaa aaaagcgact ttgtaaagtc
     4561 gctttttttg ttttaaatac cttagcgttt tttcaattag agtataatat tttcaataaa
     4621 tacaattatt ttcaattcta tttgaaaatt actccattta atacaagcga gtttgctacg
     4681 gcagagtgat acttgaataa tagtttcttg caaaacctac agcctgatca aatatcggtc
     4741 ttgtaaattc aataaacaaa tcttgttccg gaggaggcat aatgggtttt gtttctgtca
     4801 ttttaagttg tttatttgtc aatgcgcgtt catctccttg ataactagcc cattcattta
     4861 cccatacaaa tctacccggg aaattgcgac tctcttctac tttgttatta taagcattaa
     4921 ttatttttgc cgaaaggatt ccttctgaaa taacttccct agtataaaca gtcaattttg
     4981 cctttactgt tccatacaca ttttgtttta ctccattaac ggtagttgtt cccaccaata
     5041 cactatcacg ggaaatttcc gaattgtttg tagtttcacg taaattccct actgtaaagt
     5101 cttcaaaact aagcactaaa tattggtcgg gtttgtctaa accttcctct tttgcttccc
     5161 cgtaagtata gaaacggact aattgattgg ctgttattct actcatctgt gccatcagat
     5221 tatcatagaa gaaatcggca gaaagctgat aacgtttggg tgtaataggt ttttgtacaa
     5281 ccactttgag cgtagctgca tataatgcaa cttgaatttt ttccctcaca tccttataat
     5341 caggcatgta gcgttcggct tttaagaact ggtaatatgc ctcccgagca tctatgaggg
     5401 tatttagttg taataaacgt tcgccctggt tataggattc ctcaaatgct aatggcaaga
     5461 tttctctcag ctctttgcta tattgctcag gacgcgggaa aagatccatg gctttcgggc
     5521 aggtatagat tgcatctgct atagcgttga gaaccaaata ctcatctgct ataatcgaat
     5581 acttgccagc tacatcgaca tctaaggcat tttgaatttt tcgcatgctg ttttcttttg
     5641 ccaacggata tgcttgttgc aaaacctcta tcgatttttt actatttgga tttgagcgta
     5701 aagtcttaat agcacccatg gtggcgttat aataatcacc acgttgcaag gttcttttat
     5761 tcgaagaaca agtgacgctc aaaaaagtta caacaatcaa tactaacaga taactaagct
     5821 ttttcatact aatattttat ttttattttt tgtagataga acctttgatg attaaaagaa
     5881 tcatttcc
//
LOCUS       DAOSIE010000075         5873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_12271_length_5873_cov_4.467858, whole genome shotgun sequence.
ACCESSION   DAOSIE010000075 DAOSIE010000000
VERSION     DAOSIE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12271_length_5873_cov_4.467858"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="PLI69_03410"
     CDS             complement(<1..1232)
                     /locus_tag="PLI69_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOG41569.1"
                     /translation="MDYKIRLADNILTDKLEAMGAVVIEGPKYCGKTTMATQQAKSVM
                     SMADPDTKDQNLAMAATNIKRLLIGETPRLIDEWQIAPQFWDAVRNEVDKRGKAGQFI
                     LTGSAVPPNTDDIFHSGTGRMGWLRLRTMSLWESGDSTGDVSLGSLFNASEHIDGTNN
                     INLDMLAFLVCRGGWPKATLIESEKIALIQAKEYYEAVIRFDISRVDNVQRDSELAKR
                     LMRSYARNQGTQASARTILDDIRSNESEEISENTIYNYIKALKKIFVIEDSLAWNPNL
                     RSKTAIRTSDTRYFSDPSIATAALGLGPNDLINDLETFGLLFETLCVRDLRVYADAID
                     GMVYHYRDSSNLECDAVVHLNNGSYGLIEIKLGGEGPINEGAETLKTLADKIDISKMK
                     KPSFLMVLTGIGDYAYKRP"
     gene            complement(1437..2324)
                     /locus_tag="PLI69_03415"
     CDS             complement(1437..2324)
                     /locus_tag="PLI69_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOG41570.1"
                     /translation="MKYISVTEYAKRHGIPERTVRHYCKTGQIEGAYLVGKTWNIPPD
                     AVRQLQESDQRKVSPLLKAMREQQQIGLKGGIYHRTQIDLTYNSNHIEGSRLTHDQTR
                     FIFETNTIGVEGESLRVDDIIETTNHFRCIDLIIERAEERLTESLIKELHLMLKSGTS
                     DSRMEWFAVGEYKLLPNEVGGMPTAAPEKVSGEMKALLKEYNSRKINSFEEILDMHHR
                     FEAIHPFQDGNGRVGRLIMFKECLANGHVPFIITEELKMFYYRGLKEWNQTRGYLLDT
                     CLTAQDNYKALLDYFKIKY"
     gene            complement(2399..3790)
                     /locus_tag="PLI69_03420"
     CDS             complement(2399..3790)
                     /locus_tag="PLI69_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOG41571.1"
                     /translation="MFSKETYISRREKLKKHFQSGILLFLGNDECGINYEDNTYFYRQ
                     DSSFLYYFGISKPNLAAIIDIDADKEYIFGDEATIDLIVWTGSQPSIRELADRVGVKH
                     TVGLSRLTEKLGKADKEKIHYLPPYRPEHILKLNHFLGYSKEEISAKVSKKFIEAIAN
                     QRNIKSEEEIVEIEKAVTVTSEMHKRAMNFALPGMTEAQVAAVVHETALAAGGNLSFP
                     IIATINGQFLHNHYHGNTIKEGQLFLLDAGYETPLGYAGDMSSTFPVSEKFTERQKEI
                     YQITLEAHNNAIKMAKPNVNFKEVHRHVCETIFEGLKALGFTKGNTQEAVANGAHALF
                     FPCGTGHLMGLDVHDMENLGEVIVGYDNVPKSTQFGLKSLRLGRPLEPGFVITIEPGI
                     YFIPELIDHWRANKINTDFINFDKVDKYRDFGGIRNEEDLLITETGNRILGKPLAKTI
                     EDVEAERKVAFMK"
     gene            complement(3889..>5873)
                     /locus_tag="PLI69_03425"
     CDS             complement(3889..>5873)
                     /locus_tag="PLI69_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOG41572.1"
                     /translation="SVALSELLNREIDPKNLPIRRLRPEKDSLFFTIDDVDHFMILSD
                     YKIAKCEKQNNYIRPYWGLIDKEDAKVTVLSPDGKKEAYISEGNLWVKDIETGERKKL
                     SNDGSPYAYYSSKIYWSPDSHKIVCSKYSPVEYRKLLLIASSPTTQLQPLTEEYEYPK
                     PGDALPVRRPALFMVETGEIIHFDIPNVEQQYYLGNIRWNKNSEFFTFDFNRRGHQQY
                     VIYAGNLSGELRPVVNEECDTFIYYYSLYQHWFENSDEMLWISERDGWRHLYLYDVLT
                     GKVKKQLTKGEWIVKDVVKVDEKKREILFIGCGMDKGEDPYLEKYYLLNIDKGKIVAL
                     TPENAHHSVTFSHDYAYMFDSYSRVDLPPTLVVRSVEKGGKVIYKPDLQPDISAVTAT
                     GWDLPEVFCAKGRDGKTDIWGIIVRPSNFDPSKKYPVIEYIYAGPHDSHVPKHFAIEQ
                     RYTSALAELGFILVMIDGMGTANRSKAFHDVCFQNLKDAGFPDRIAWIKAAAAKYPEM
                     NIDNMGIYGMSAGGQSTMGALLFHPEFYKVGVSACACHDNRMDKIWWNEQWMGYPIGE
                     HYAASSNMENAHLLEGKLLLIMSDLDNNVDPSSTMQVVRALIKHDKEFDLVMLPGERH
                     TMGGKYGERKRRDFFVKHILNIEPPQWNDKNTGAEK"
BASE COUNT         1543 a         1339 c         1150 g         1841 t
ORIGIN      
        1 ggtctcttat aggcataatc tccaattcct gtaagaacca taagaaaaga aggttttttc
       61 attttgctaa tatctatctt atctgccagt gtttttagtg tttccgcgcc ctcattaatt
      121 ggaccttctc ctcccaattt aatctcaata agaccgtaag aaccattatt caaatgaaca
      181 acggcatcac attcaagatt gctgctatct ctataatggt aaaccatacc gtcaatagca
      241 tccgcataga cacgaagatc acggacacaa agagtctcaa acaataaacc gaaagtttct
      301 aaatcattta ttaaatcatt tgggccaagt cccaacgctg ccgtagcaat tgaagggtcg
      361 gaaaaatatc gcgtgtcgct tgtgcgaatg gctgttttac ttcttaaatt ggggttccag
      421 gctaatgagt cttcaatgac aaatattttc tttagagcct ttatataatt gtaaatagta
      481 ttctcactga tctcttcact ttcattactt ctaatatcat caagtatcgt ccttgcgctt
      541 gcctgtgttc cttgattcct tgcataagaa cgcataagcc tctttgccaa ttctgagtct
      601 cgctgcacat tatccactct tgaaatatca aaccttatga ctgcctcgta atattctttt
      661 gcctggatca gagcaatttt ttcggattct attaatgttg ctttcggcca tccacctcga
      721 cacacaagga aagccaacat atccaaattg atattattag ttccgtcaat atgttctgat
      781 gcgttaaaaa gtgaccctaa acttacatct cctgttgaat cccctgattc ccaaagactc
      841 atagtacgca gtctcaacca tcccatgcgt ccggtaccgg agtgaaatat gtcatcggta
      901 tttggaggta cggcagaacc tgtgagtata aactgtccgg cttttcctct cttatccact
      961 tcgtttcgta ccgcatccca aaactgcggg gctatttgcc attcatcaat caggcgaggt
     1021 gtttcaccta ttagtaaacg ttttatgttt gtagcggcca tggcgaggtt ttgatctttt
     1081 gtgtcagggt ctgccataga cattacactt tttgcctgct gtgtagccat agtagtctta
     1141 ccacaatatt taggaccttc tataacaaca gcccccatcg cttccagttt gtcagtaaga
     1201 atattatcag caagtctaat tttgtagtcc atatttcaca tgtatatatt cacaaatata
     1261 gaaactcttt ttacaataat aaaggaattt aatagcgaat gtgacagttg aggctttttt
     1321 gatgtgacag ttgaaggatt tttgatgtga cagttgaggc ttatttgatg tgacagttgc
     1381 attatttgga aaaatatgga attttgcaga aacgatctgt gtgcatacca tccatttcaa
     1441 tacttgattt tgaagtaatc gagcagtgct ttataattat cctgcgctgt cagacaagta
     1501 tcaagcaaat agccgcgggt ttgattccat tctttcaatc cccgataata gaacatcttc
     1561 aactcttccg ttatgatgaa tggtacatgc ccgtttgcca gacactcctt gaacataata
     1621 agtcggccta cacggccatt gccatcctgg aaaggatgta tcgcttcaaa tctatgatgc
     1681 atgtcgagta tctcctcaaa ggaattgatt ttccttgaat tgtactcttt tagcaacgcc
     1741 ttcatctccc cactcacttt ttccggagct gctgtcggca tcccaccaac ctcatttggc
     1801 aaaagcttgt attctcctac tgcaaaccac tccatccggc tatcggaagt acccgacttt
     1861 aacatcaaat gcaactcctt tatcaaactt tctgtcaatc tctcttccgc tctctctata
     1921 atcaagtcaa tgcagcgaaa gtgatttgtt gtttcaataa tatcatccac ccgcagactc
     1981 tccccttcca ctcctatagt attggtttca aaaataaacc ttgtctgatc atgtgtaagg
     2041 cgactccctt cgatatgatt ggaattgtaa gtgagatcta tctgcgtgcg atggtaaatg
     2101 ccacccttta gaccaatttg ctgctgctca cgcatggcct tcaaaagtgg cgacactttg
     2161 cgttgatcac tctcttgcaa ttgtcgaaca gcatcaggag gtatattcca ggttttgcca
     2221 acaagatatg ctccttctat ttgacctgtt ttgcaataat gtctgactgt tctttccgga
     2281 atgccgtgcc ttttggcata ttccgttact gaaatatatt tcataatatt tacctatcgg
     2341 caaattaaaa gccgaatact atagcaaata tagggatttt tgccgatacc ggcaaaaatt
     2401 atttcataaa ggccactttt ctttcagctt ctacatcttc gattgtttta gccaaaggtt
     2461 tgccgagaat acgatttcct gtttccgtaa ttaagagatc ttcttcgttg cggatgccgc
     2521 cgaagtcgcg gtatttgtca actttgtcga aattgatgaa gtcggtattg attttattcg
     2581 ctctccagtg atcaatcagt tcgggaatga aatagattcc cggctcgatg gtgattacaa
     2641 agccgggctc gagaggcctg cccaatcgga gtgatttcag gccgaattgg gtgcttttag
     2701 gcacattgtc atatcccacg atcacctctc ccaaattctc catatcgtgc acatccaatc
     2761 ccatcaggtg acctgttccg caagggaaga atagtgcatg tgctccgttt gcaacagctt
     2821 cctgcgtgtt gcctttagtg aatccgaggg ctttcagtcc ctcgaaaata gtttcgcaga
     2881 catggcggtg cacctctttg aagttgacgt tgggcttcgc cattttaatg gcgttgttgt
     2941 gagcttccaa tgttatttga taaatctcct tctggcgttc ggtaaatttt tccgaaacgg
     3001 gaaatgtact gctcatatct ccggcatatc ccagtggagt ttcataacct gcatccagca
     3061 aaaagagttg accctctttt atggtgttgc cgtggtaatg attgtgtagg aattgtccgt
     3121 tgatagtggc gataataggg aaagagagat ttccgcccgc agcaagtgcc gtttcatgga
     3181 ccaccgcagc cacctgggct tccgtcatgc ctgggagtgc gaaattcatg gctcttttgt
     3241 gcatctccga agttacggtt actgcttttt caatttccac aatctcttcc tccgatttga
     3301 tgttgcgttg attggctatc gcttcaataa acttcttgct gacttttgcg gagatttctt
     3361 cctttgaata gcccaaaaaa tgattgagtt tcaaaatatg ttcgggacga tatggaggca
     3421 ggtagtgaat tttctcctta tcggcttttc cgagcttctc agttaatcgc gataagccga
     3481 cggtgtgctt aactcctacc ctatcggcca actcccggat gcttggctgt gaaccggtcc
     3541 aaacaatcaa gtcaatggta gcttcatcgc cgaaaatata ctctttgtct gcgtcaatat
     3601 cgattattgc agccagattg ggcttgctta ttccgaaata atagaggaaa ctgctgtcct
     3661 gacgataaaa ataggtgttg tcttcataat tgattccaca ctcatcattg ccgagaaaaa
     3721 gcaggattcc cgattgaaaa tgttttttca atttttctct tctcgagata taagtttctt
     3781 ttgagaacat aggcttatgt atttattgtt gttattgatc attcgcacct aattgctatt
     3841 gctcccgtag cgaagagagc aacacaagcg tgcgaaagta gttatctgtt acttctcagc
     3901 accggtgttc ttgtcgttcc attgcggcgg ttcgatattg agaatatgct ttacaaagaa
     3961 gtcgcgccgt tttctctcac catattttcc acccatggta tgcctttctc ccggaagcat
     4021 cacaagatca aactccttat catgtttgat tagcgctctt accacctgca ttgtgcttga
     4081 ggggtcaacg ttattgtcca aatcactcat aataagcagc aattttcctt ccagaaggtg
     4141 agcattctcc atattggaag aggcggcgta atgttcgccg atagggtaac ccatccactg
     4201 ctcattccac cagattttat ccattctatt atcgtgacat gcacacgctg aaacgcccac
     4261 tttgtaaaat tcgggatgga acagaagtgc acccatagtg ctttgtccgc ctgccgacat
     4321 accgtaaata cccatattat cgatattcat ttcgggatat ttcgcggcag cagccttgat
     4381 ccaagcaatc ctatcgggga aaccggcatc tttcaaattt tggaagcaaa catcgtggaa
     4441 ggctttcgag cggttcgccg ttcccattcc atctatcatt acaagtataa atccgagctc
     4501 agcgagagca gaagtatatc gttgctcaat agcgaaatgc ttaggcacgt gggaatcatg
     4561 tggtcctgca tagatatact cgataacggg atattttttc gaaggatcga aattagaagg
     4621 acgaacgatt atgccccaaa tatcggtttt cccgtcacga cctttggcgc agaaaacttc
     4681 gggaagatcc catccggtag ctgttacggc cgaaatatca ggctgcaaat ctggtttata
     4741 gatgactttt ccgccctttt cgactgaacg gacaaccaga gtcggcggca aatccaccct
     4801 cgaataagaa tcaaacatat aagcgtaatc atgcgagaaa gttacactat ggtgagcatt
     4861 ttcaggagtg agagccacta tttttccttt gtctatattt agcaagtaat atttttcgag
     4921 ataagggtct tcacctttat ccattccgca ccctataaag aggatttctc tctttttttc
     4981 atccacctta accacatctt taacaatcca ctcacctttt gtcagttgct ttttaacttt
     5041 tcctgtaaga acatcgtaga gatagaggtg tctccaaccg tcgcgttcag agatccaaag
     5101 catttcgtcg gaattttcaa accaatgttg atacaaagaa tagtaataga tgaaggtgtc
     5161 acactcttca ttgacaacag gccgcagttc tcccgataga tttccagcat aaattacata
     5221 ttgctgatgt ccgcgtctgt tgaaatcgaa agtgaaaaat tcgctgtttt tattccacct
     5281 gatattgccc agataatatt gctgctccac attgggaata tcgaaatgga ttatttcacc
     5341 cgtttccacc ataaaaaggg caggccgtct tacgggcaga gcatcacccg gcttcggata
     5401 ttcatactct tccgtgagag gttgcaattg agttgttggc gatgaagcga ttaaaagcaa
     5461 tttcctgtat tccacaggac tatatttgct acacacaatt ttgtgcgagt caggcgacca
     5521 atagatcttt gaagagtagt aagcgtaggg cgaaccatcg ttgctcaatt tctttctctc
     5581 tcccgtttca atatccttta cccagaggtt tccttcggaa atataggcct cctttttgcc
     5641 atcgggagag agcacggtta cttttgcatc ctccttgtca ataaggcccc agtaaggtct
     5701 gatgtagtta ttttgtttct cacatttagc tattttgtaa tctgacaaaa tcataaagtg
     5761 atccacgtca tcaatagtga aaaagagcga atctttttca gggcgcaggc gccgtatagg
     5821 aagatttttg ggatcaattt cccgattaag gagctcagaa agtgctacgg aga
//
LOCUS       DAOSIE010000076         5707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_12717_length_5707_cov_3.516100, whole genome shotgun sequence.
ACCESSION   DAOSIE010000076 DAOSIE010000000
VERSION     DAOSIE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12717_length_5707_cov_3.516100"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="PLI69_03430"
     CDS             <1..1232
                     /locus_tag="PLI69_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOG41573.1"
                     /translation="KPDFGPAVFNDRVAHTGCTMIGARDFLIAINYNLNTTSTRRANA
                     IAFDVREKGRKKREGDPITGKVVKDENGKDVWIPGTLKGCKAIGWFIEEYGIAQVSMN
                     VTDISQTPVHIAFDEVSRAAQNRGIRVTGSEIVGLIPKKVLIDAGKYYLKKQQRSLGI
                     SEEEIIKIAVKSMGLDDLKPFNPREKVIEYLMEDAEAAGAPRKLVDMTCAGFAFETAS
                     ESPAPGGGSISAYMGALGAALGTMVANLSSHKPGWDADWEKFSEWAEKGQQIMAQMLH
                     LVDEDTNAFNRIMEAFSLPKGSDEEKAARAAAIQDATLYATRVPLETMKTAFTAFDLL
                     DAMAAEGNPNSVSDAGVGALAVWSAVHGAWLNVKINAAGLKERATADALLAEAAAIAA
                     EADVRREHVIAICNEKI"
     gene            1270..3261
                     /locus_tag="PLI69_03435"
     CDS             1270..3261
                     /locus_tag="PLI69_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HOG41574.1"
                     /translation="MNRANASFDENELRYLKLLSKSFPTIAEASTEIINLQAILNLPK
                     GTEHFLTDIHGEYEAFRHVLKNASGTIERKVNEEFGDTLRELEKRDLCTLIYYPRERL
                     AIVKSRESHIDEWYMITLIQLVKICQRVSSKYTRSKVRKAMPKKYSYIIEDLMHESLS
                     DPNKQAYLKNILSNIIELDRADDFIIALCDLIQRLAIDSLHIVGDIYDRGPGAHIVME
                     TLINYHDVDVQWGNHDILWMGAASGNDSCIANVIRICMRYGNLRTLEEGYGINLLPLA
                     TFAMHTYAGDPCNAFQPRLHFADDKYSNRSTHLIAQMMKAISILQFKLEAAVIERRPE
                     FEMADRNLFHNVNFEKGIITIDGVEYPMLDNNFPTVDPADPYRLTSEEEELVAKLHRS
                     FVSCDRLCRHIDFLMSKGSLYLVRNGNLIFHASIPLNEDGTFKSVKLDGVDYSGKALL
                     DKVDTLIRKAYYGEEETPEHSFAVDYMWYLWCGPDSVAFDKKKMTTFERSFIADKALH
                     KEPKGHYYTLREKESVCDMILDEFGVKGRNRHIINGHVPVKTIKGESPIRAGGKMLII
                     DGGFSRAYQSETGIAGYTLIFNSHGMQLVQHEPFTSTRQAIEHGVEVKATRFVVDYTT
                     QRMLVKDTDNGEEIGGQIAALLRLLEAYRTGLIKETIKG"
     gene            3334..5673
                     /locus_tag="PLI69_03440"
     CDS             3334..5673
                     /locus_tag="PLI69_03440"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HOG41575.1"
                     /translation="MNQLFKKILIAAAMLLLFFADITAQAQTQTIKGKVIDENNLPVA
                     YANVVLLKADSTFVNGTVTDTSGYFSVEATSKSDLLRISFMGYDAHCTVVDCDDVGII
                     ELQPCSEILCGALLKSQLPKTVLRNDAFVTPIKGCVLSKAGSANDVLKRLPGVIGKNG
                     TFEVLGKGAPLIYIDGRMVRDNSELELLSSEDILEAEVITNPGARYDATVKAVILLKT
                     IKREGDGLSFDLRSSTFIHHGKLDWIEQINLNYRYNNLDIFASLGYDKSAPDTFSEIT
                     QSLRSQNLLELQKNLTYSNEVRTMTPVLGFNYQFNPKHSVGFRYYPYVFLGGRSTQDL
                     HIKTYLDGILDDNVQIPADGKSFAGVTQRANVYYNGTVGDLEIDFNADIQHGGALDSI
                     VFNERSEMKESRDVHTINGMANRLMASKLVLSYPLLGGKLSAGSEYTWTEYEEDYRNP
                     EKFVPNIDNKLIEHNAAAFAEYSHPLPFGSFSAGVRYEHADSGFEENGKYDEERSRKY
                     NHFFPHASLSAVAGPVQLQLSYTAKARRPLYHQLTNISSYLGRYGISRGNPTLKPQIS
                     QDLSLSAVWKFMQLSVSYQVFKDAIINAATAQPDMPNIIVIRPINYGKNFPMINARVS
                     ASPTIGFWTPRISLGINKQWLTLSYMGEDVELTKPVLLFAIGNTFSFPKGFMLNVEYH
                     LQGKGHILAYEMVEPLHELEIALSKSFFNDALTVELRGLDLFALRHEKRHLISGVYQI
                     LESTRLDSNELVITIRYKFNNVKSKYKGTGAGESERKRL"
BASE COUNT         1621 a         1209 c         1339 g         1538 t
ORIGIN      
        1 ggaaacccga ttttggccct gctgtattca atgatagagt ggcacacacc ggctgcacta
       61 tgattggagc tcgtgatttt cttatagcaa tcaattacaa tctcaacaca acctccaccc
      121 gtcgcgccaa cgccatcgct tttgacgttc gggaaaaagg gagaaaaaaa cgtgagggcg
      181 accccattac gggtaaagta gtaaaagatg agaatggtaa agatgtttgg atccccggca
      241 ctctaaaggg ctgtaaggcc attggttggt ttatcgaaga gtacggtatc gcacaagtat
      301 cgatgaatgt gaccgacatt tcccagacgc cggttcatat cgcctttgat gaagtaagca
      361 gggccgcgca aaatcgaggt atccgggtga caggctccga gatagttggc ctaattccca
      421 aaaaggtact cattgatgca ggcaagtatt atcttaaaaa acaacaacgc agcctcggta
      481 tttctgaaga agagataatc aagattgcgg taaaatcaat gggactggat gacctaaaac
      541 ctttcaatcc tcgtgaaaag gtaattgaat atctgatgga agatgcggag gcggcaggtg
      601 ctccccgaaa attggtggat atgacatgtg ccggttttgc atttgagacg gccagcgagt
      661 ctcctgcccc cggtggcggc tcgatctcag cctatatggg agctcttggt gcagcgttgg
      721 gtacaatggt ggctaatctt tcttcacaca agcccggctg ggatgccgat tgggagaagt
      781 tttcggaatg ggctgagaag gggcagcaga tcatggcgca aatgctccat ttggtggatg
      841 aagatacgaa tgcattcaat cgcataatgg aggcattttc actgccaaag ggctccgatg
      901 aggagaaagc agcgcgtgcc gcagctattc aggatgccac cctttatgcc acgcgtgtgc
      961 ctcttgaaac gatgaaaact gcatttacag cctttgacct cttggatgcg atggctgccg
     1021 aaggcaatcc caactcagta tcagatgcag gtgtgggagc attagccgta tggtctgcag
     1081 tgcatggcgc ctggctaaat gttaagatca atgcagcggg attaaaagag cgtgctacag
     1141 ctgatgctct tttggcagaa gcagctgcga ttgcagcaga ggctgatgta cgtcgtgaac
     1201 atgtgatcgc catctgtaat gaaaaaatct gataccgcat tattttaact taaccttaat
     1261 gctataaaaa tgaatagagc taatgcctca tttgacgaaa atgaactcag gtatctgaaa
     1321 ctattgtcga agagtttccc aaccatagct gaagccagta cggagatcat taatcttcag
     1381 gcaatactca accttccaaa ggggacagaa cactttctta ctgacataca tggtgagtat
     1441 gaggcattcc gtcacgtgct gaagaatgcc tcaggaacca tcgaacgcaa ggttaatgag
     1501 gaatttgggg atacattgag agagctggag aaacgcgatt tgtgcacatt gatatactat
     1561 ccgcgtgaaa gacttgcaat agttaaatct agggagagcc atattgacga atggtatatg
     1621 attacgctga tacaactggt gaaaatctgc caacgggtct cttccaagta cacacgttca
     1681 aaagtgagaa aggcaatgcc caaaaagtat tcctacataa tagaggatct gatgcacgag
     1741 tctctttcag atcctaacaa gcaggcttac ttaaaaaaca ttctttccaa cattatcgaa
     1801 cttgataggg cagatgattt tataatagct ctttgtgatc tgattcaacg tctggctata
     1861 gattctctac acatagtggg agacatatat gacaggggtc cgggtgctca tattgtaatg
     1921 gaaactctga ttaactatca cgatgtggat gtacagtggg gcaatcacga tatcctttgg
     1981 atgggagctg catctggcaa tgatagctgt atcgcaaatg tgatacgcat ctgtatgcgt
     2041 tacggcaatc tcaggacact tgaagaggga tatggaataa atctactgcc tttggctacc
     2101 tttgctatgc acacctatgc aggagatcct tgcaatgcct tccagccacg tctccatttt
     2161 gccgatgaca aatacagcaa ccgtagtacg catctgattg cccagatgat gaaagctatt
     2221 tcaattttgc agtttaaact cgaggcagct gttattgagc gcagaccaga gttcgaaatg
     2281 gctgaccgca accttttcca caatgtaaat tttgagaagg ggataataac tatcgatggt
     2341 gtggaatatc cgatgctgga taataatttc cctactgtag atcccgcaga cccctatcgt
     2401 cttacttcgg aagaggagga gcttgtggct aaattgcacc gttcttttgt cagttgcgat
     2461 cgtctttgca gacatataga ttttctgatg agcaaaggaa gtctctacct cgttagaaac
     2521 ggtaacttga tatttcatgc ctccattccg ctcaatgagg atggtacttt caagagtgtg
     2581 aagttggacg gagtcgacta ttcagggaaa gcattattgg ataaagtgga tactttgatt
     2641 agaaaggctt attatggaga agaagagact ccggagcaca gttttgccgt tgattatatg
     2701 tggtatctct ggtgtggtcc cgattcggtt gcgtttgaca aaaagaagat gaccactttc
     2761 gaacgctctt tcattgccga taaggcgcta cacaaagagc caaaaggaca ttactatact
     2821 cttcgtgaga aagaatcggt atgcgatatg attttggatg aattcggcgt caaaggcaga
     2881 aatcgtcata tcataaatgg ccatgtacct gtcaagacta tcaagggaga gtctccaatc
     2941 cgtgcaggtg gcaagatgct gattattgat ggcggttttt cacgcgcata ccagtcggaa
     3001 accggtatcg cgggttatac cctgatattc aattcccacg ggatgcaatt agtacagcat
     3061 gagcccttca catctactcg tcaagctata gagcatggtg ttgaagttaa agcgacacgc
     3121 tttgttgtcg attatacgac acaaaggatg ctcgttaagg ataccgataa tggtgaagag
     3181 ataggcggtc agattgccgc actgcttcgc ctgcttgaag cctaccgcac cggactcata
     3241 aaagagacaa taaaaggttg aatttctctt ttaatattgc aacaatcctt tccttccagc
     3301 gtcttataca atagtagcaa ccgtttttaa tctatgaacc aacttttcaa aaagatactt
     3361 attgcggcag ctatgctgtt gctctttttt gccgacatta cagcacaggc acaaactcag
     3421 actattaaag gtaaagtaat tgacgaaaat aatctccctg ttgcttacgc taatgtggta
     3481 cttctaaaag ctgattccac ttttgtcaac ggcacggtta ccgacacctc cggatacttc
     3541 tccgtggagg caacaagtaa atcggacctg ctaaggattt catttatggg atatgacgca
     3601 cattgtactg tggtagattg tgacgatgta ggtattatag agttgcaacc ctgttcggag
     3661 atattatgtg gagcattact taagagtcag cttcccaaga cagtgcttcg caatgatgct
     3721 tttgttactc ctataaaggg ttgtgtactt tccaaagccg gttcggccaa tgatgtgttg
     3781 aaaagactgc cgggagtaat aggtaaaaac ggtactttcg aggtcttggg taagggtgct
     3841 ccattgatat acattgatgg gcgaatggtg cgtgacaatt ccgaattgga gctgctcagt
     3901 tccgaagata tcctcgaggc ggaggtgatc accaatccgg gtgcccgtta cgatgccacc
     3961 gtaaaagcgg taatcctact taaaacaatt aagagagagg gtgacggact cagctttgat
     4021 ctccgttcaa gtacctttat tcatcacgga aaactagatt ggattgaaca gatcaacctc
     4081 aactatagat ataacaatct ggacatcttt gcctctcttg gatatgataa atcagcgcct
     4141 gacacttttt ccgagataac tcagagcctc cggtcacaaa atctgctcga attgcaaaag
     4201 aatttgacat attccaacga ggtacgaacc atgactccgg ttttgggatt caactatcag
     4261 ttcaatccta aacattcagt tggatttcgc tactatccct atgtgttttt agggggtaga
     4321 tcgacacagg atcttcatat aaaaacctac ttggatggga ttctcgatga taatgtccaa
     4381 ataccagcgg atggtaagag tttcgcgggt gtcacccagc gggcaaacgt ttactacaat
     4441 ggtaccgttg gtgatcttga gattgacttc aatgcagaca ttcagcatgg aggtgcgctt
     4501 gatagcatag tatttaatga gcgaagtgaa atgaaggaga gtcgtgatgt gcatactatc
     4561 aacggtatgg caaaccgtct tatggcctct aaacttgtgc tttcctatcc gttgctcgga
     4621 ggaaaactct ccgccggaag tgaatatacc tggaccgaat atgaggaaga ttaccgcaat
     4681 cctgaaaaat ttgtgcccaa catagataat aagttaatag agcataatgc cgctgctttt
     4741 gcagagtaca gtcatccact ccccttcggt tctttttcag ccggagtgcg gtatgagcat
     4801 gctgattccg gtttcgaaga aaatggaaag tacgatgaag agaggagtcg caaatacaac
     4861 catttcttcc ctcacgcatc tctgagtgca gtcgcaggac cggttcagct gcaactgagt
     4921 tacactgcca aggctcgtcg ccctctctat caccaattga ccaatatctc atcctatttg
     4981 ggcaggtacg gtatttccag aggaaatccc acactcaaac ctcaaataag ccaagacctc
     5041 tctttgtctg cggtttggaa atttatgcaa ttgtccgtca gctaccaggt cttcaaggat
     5101 gccattataa atgcagctac ggctcaaccg gatatgccta atattatcgt gattcgtccc
     5161 attaactacg gcaaaaattt tcccatgatt aacgccagag tgtcagcctc tccaaccatt
     5221 ggtttctgga cgcctcgtat ttctctcggc attaataagc agtggcttac gctcagctat
     5281 atgggagaag atgttgaact gacaaaaccg gtcttacttt tcgccattgg aaacactttc
     5341 tcttttccta aaggtttcat gctcaatgtg gaataccatt tacagggtaa ggggcatatt
     5401 ttagcttatg agatggtaga gccactgcac gaactggaga tagctttgag caaatctttt
     5461 ttcaatgatg ctcttacggt ggaactaagg ggtctcgatc tctttgcact cagacatgaa
     5521 aaaaggcatc tcataagcgg agtgtatcag atacttgaaa gcacgcgttt agattccaat
     5581 gagcttgtca tcactattcg ctacaaattc aacaacgtaa agagcaaata caaaggtacc
     5641 ggtgccggcg aaagtgaaag gaagaggcta taaggcttga tttccttact tttttctatc
     5701 tttgccg
//
LOCUS       DAOSIE010000077         5701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_12738_length_5701_cov_3.893022, whole genome shotgun sequence.
ACCESSION   DAOSIE010000077 DAOSIE010000000
VERSION     DAOSIE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12738_length_5701_cov_3.893022"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLI69_03445"
     CDS             <1..415
                     /locus_tag="PLI69_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOG41576.1"
                     /translation="ICGILIENGISCDKLAYSIAGIGININQEKFDSNAPNPTSLKLI
                     TGKEYDIQQELPDIVCLIAGRYNQATTGKTNELEKDYLELLYRKGEYRNYIDCTTGDS
                     FEARITGLDDQARLKVETRDGEIKVYAFKEIGYVI"
     gene            complement(390..1046)
                     /gene="rpe"
                     /locus_tag="PLI69_03450"
     CDS             complement(390..1046)
                     /gene="rpe"
                     /locus_tag="PLI69_03450"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOG41577.1"
                     /translation="MSLIIAPSMLSADFANLSDDIRMVNESRADWFHLDIMDGLFVPN
                     ISYGFPVIKAIAALAEKPLDAHLMIMNPDRYIDRFAELGVSWLSVHYEACTHLHRTVQ
                     RIKSAGMKAGVAINPHTPVSCLEEIIPYCDYILVMAVNPGFGGQSFIPTTVDKVAKLY
                     AMTERLNPDCIIEVDGGVNEANIASLAAAGAKAFVAGNSVFTAENPSAMIAKLRNQSL
                     "
     gene            complement(1059..2495)
                     /locus_tag="PLI69_03455"
     CDS             complement(1059..2495)
                     /locus_tag="PLI69_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOG41578.1"
                     /translation="MKSEKVLSFFKEITRIPRESGHEEQIIEYLQQFAASHNLECKTD
                     KVGNVLIIKEASKGYENSPTVILQSHSDMVCEKNSDSTHDFSKDPIKYVIEDGWMIAK
                     ETTLGADCGIGIAAQLAILEDKSLEHGRIEALFTVEEETGLDGAKALKPGFLTGNILI
                     NLDSEDEGELFVGCAGGIDTTARFKYKPKKSDPSKVYTRFTVLGGMGGHSGDDIEKGR
                     ANAVQLLGRFLNRCFAEFKIDLCSIDGGNKRNAIAREASAVVAIPARSEDAVIELFNA
                     FATEIRSEYTVTDPAVNAVAEPVAREKNAIPAGVAKRLVAALVAAPHGVLAMSAELKG
                     LVETSSNLASVKMGKGNTIRIGSSQRSSINSARRFAGDRFAAVFRLAGAQVAHESEYP
                     GWKPNMNSPLKDVCVDAYVRLFGQQPVVRAIHAGLECGLFLDTYPNLDMISFGPTLRG
                     VHAPGERLELASVDKFWLLLLEVLRSIK"
     gene            complement(2535..3971)
                     /gene="dnaA"
                     /locus_tag="PLI69_03460"
     CDS             complement(2535..3971)
                     /gene="dnaA"
                     /locus_tag="PLI69_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOG41579.1"
                     /translation="MSLSNHIDVWNRCLAIIRDIIPKEQYDTWFHPVRSVSLENSTIT
                     LAVPSHFFREYLEEHYIDVLNLVLRREIGPQVTLIYAVTVVEGEQVRLPEQNSKTYHN
                     NEIPVPEVGQNYNGNPYIIPGLKKLKIDPQLNPQYSFANFIEGECNRLGRAAGMNISI
                     NPGRSTFNPLFIYGGPGLGKTHLAQAIGIAIKEKYPEKVVLYVSANVFQNQYTSAACS
                     AKSNNNNTSVADFLRFYQLIDVLIIDDVHEFADKKGTQNAFFHIFNYLHQSGKQLILT
                     SDKPPVELQGLEQRLLSRFKWGLSAELQMPSYNTRLEILKSKSEREGVDIPEEVLSFI
                     AKKVKTNIRELEGTLFSLIASATFGRKEITLELARNLVDKIVTEHKVEITINRIQNIV
                     CNYFNITTDQFLSKTRKREIVQARQIAMYLSRNLTKTSLASIGSQLGGKDHATVHHAC
                     NTVCDLMDTDRNFRQYVVDIEKQVTMID"
     gene            complement(4205..5269)
                     /locus_tag="PLI69_03465"
     CDS             complement(4205..5269)
                     /locus_tag="PLI69_03465"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG41580.1"
                     /translation="MKLRTVVIDAGHGGKDPGAISSDNKIREKTITLDVALKLGQRIK
                     DAYPDVKVVYTRSKDTYLTLAQRTNIANRNQADLFISIHVNSVPSSKPRGSETYIMGT
                     DESHSNMEVCKKENSVILLEDDYTTTYQGYDPNEPESFIFFNLMQNAHFEQSVIMASK
                     IQEQLRKGPITVDRGVKQALLLVLWRTSMPSVLVEIGFLSNAADRKQLASESGRKEIA
                     GRLFTAFSEFKKHYDSHSGMEVDPPSVSTGVSSEKEKPVSSASTDLSSEKEKPVSSES
                     TSSDWWMIQIFASKKQVKPGAPDFKGLNNCVEIFQNGYYKYCTGRFATREEAVKALPA
                     LRKKFEGAFIVHVDKNNRIL"
BASE COUNT         1592 a         1316 c         1221 g         1572 t
ORIGIN      
        1 gatatgtggc atcctcattg aaaatggtat ttcatgtgac aaattggcat atagcatcgc
       61 cggcatcgga ataaacatca atcaagaaaa atttgactcg aatgctccaa atcccacctc
      121 tctgaaactt ataaccggca aagaatatga catacagcag gaactccccg atattgtctg
      181 tctgattgcc ggacgctaca accaggccac tacgggaaaa acaaatgaac tcgaaaaaga
      241 ttacctggag ctactttacc gcaagggaga ataccgaaat tacatcgact gcaccaccgg
      301 agatagtttt gaagcacgta tcaccggcct ggacgaccag gcacgcctga aggttgaaac
      361 acgtgacggg gagatcaagg tttatgcctt caaagagatt ggttacgtaa tttagcaatc
      421 atggcagaag gattttctgc cgtaaaaacg gaattgcccg caacgaaagc ttttgcaccg
      481 gcagcagcca aagaggctat attggcctca ttgaccccac catccacctc aataatgcaa
      541 tcaggattta acctttctgt cattgcatag agttttgcca ccttatctac tgtagtcggt
      601 ataaatgact gacctccaaa tcctggattt acagccatca ccaatatgta atcgcaatat
      661 ggtataatct cctcaagaca agaaaccgga gtgtggggat taatggcaac gcccgccttc
      721 atgccggcgc ttttaatacg ctgaactgtc ctgtgtagat gcgtacaggc ctcataatgg
      781 acactaagcc aactcactcc caattcagca aaacgatcta tatatctatc gggattcata
      841 atcatcagat gagcgtcaag cggcttttca gcgagtgcag caattgcctt tatgacagga
      901 aaaccatacg agatattagg cacgaagagg ccatccatta tgtccagatg aaaccagtct
      961 gcccggctct cgttaaccat ccggatatca tccgacagat ttgcgaaatc tgcagaaagc
     1021 atcgaaggag cgataatcag tgacatagca acgttatttt acttgatgga tctcaatact
     1081 tcgagcagca aaagccagaa tttatcgacc gaagcgagct caagacgctc tccgggtgca
     1141 tggactcccc tcaaagtggg tccgaaagag atcatatcga gattaggata ggtgtcgagg
     1201 aaaagaccgc actccagacc ggcatgaatg gctcgaacca caggttgttg gccaaataga
     1261 cgcacatagg catctacaca gacatccttc aggggagaat tcatattggg cttccatccc
     1321 ggatattcag actcatgggc aacctgagct ccggccagcc ggaagacagc tgcaaagcga
     1381 tcaccggcaa atcttcgggc ggaattgatg gagctgcgct gacttgatcc gatacggatg
     1441 gtattccctt tgcccatctt gacagatgca aggtttgaag aggtctccac aagacctttg
     1501 agctccgcac tcattgcaag cactccatga ggagctgcca caagtgcagc cacgagcctc
     1561 ttggcgacac cggcagggat ggcattcttc tcccgagcca ccggttcagc tactgcgtta
     1621 acagccggat cggtcacagt atattcactc ctgatttcag ttgcaaaagc attaaagagt
     1681 tcaataaccg catcttcgct ccttgcgggg atagccacca ctgccgatgc ttcacgcgca
     1741 atggcattgc gcttattgcc accatcgatg gagcaaagat cgattttaaa ttctgcaaaa
     1801 cagcgattaa gaaaacgtcc caggagttgc acggcattgg ctctcccctt ctctatgtcg
     1861 tcaccgctat gacctcccat acctcctagt acagtaaagc gagtatagac tttggaggga
     1921 tcactcttct tgggcttata tttgaagcgt gcggtagtat caataccacc ggcgcaacct
     1981 acaaagagtt caccctcatc ctctgagtcg agatttatca aaatattacc ggttaaaaag
     2041 ccaggtttaa gtgcttttgc accatccagc ccggtctctt cttcaactgt aaaaagcgcc
     2101 tcaatcctac catgttccag cgatttatcc tcaagaatag cgagctgcgc tgcgatgcct
     2161 ataccgcaat cagctcccaa agtagtttct ttggcaatca tccaaccatc ctctatgaca
     2221 tattttatgg gatcctttga aaaatcatgc gtagagtctg aatttttctc gcaaaccata
     2281 tcagaatgac tctggagaat cactgtaggg ctattttcat accctttgga ggcctctttt
     2341 attatgagca cattaccgac tttatctgtt ttgcactcta aattatgtga tgctgcaaac
     2401 tgctgtaaat actctatgat ttgctcttca tgacccgatt cgcggggaat acgggttatc
     2461 tctttgaaaa aagataatac tttttcactc ttcataataa atggaatata tgttatcgtg
     2521 tttgtactat tgtactagtc aatcatagtg acttgcttct cgatatctac cacatactga
     2581 cggaaattgc ggtcagtatc catcagatcg catacggtat tgcaggcatg gtgcacggta
     2641 gcgtgatcct tccctccgag ctgggaaccg atggaagcga gcgacgtttt tgtaagattg
     2701 cgactgagat acatggcaat ttgtcttgcc tgaacgattt cccgcttcct tgttttggac
     2761 aagaactgat cagtggtgat attgaaataa ttgcaaacta tgttctggat acggtttatg
     2821 gtgatctcaa ccttgtgttc ggtgacaatc ttgtcgacaa gattgcgtgc caactccaaa
     2881 gtgatctcct tgcggccaaa tgtggcactg gcaataagcg aaaatagtgt accttcaagc
     2941 tcgcgtatgt tagtcttgac cttttttgca atgaaggaga gcacttcttc aggaatatcc
     3001 actccctctc tctcgctctt cgacttgaga atctcgaggc gtgtgttgta tgaaggcatt
     3061 tgaagctcag cagaaagtcc ccatttgaaa cgagacaaaa gacgctgctc aagcccctgc
     3121 agttccacag gcggtttgtc cgaggttaga ataagctgtt tgccactctg gtggaggtag
     3181 ttgaatatat ggaaaaaagc attctgtgtt cccttcttat cagcaaattc gtgaacatca
     3241 tcgattatca gcacatcaat aagctgataa aatcttagaa aatcggctac gcttgtatta
     3301 ttattgttac tctttgcaga acaggcagcc gaagtatact ggttttggaa aacattggcc
     3361 gaaacataaa gcaccacctt ttcgggatac ttctccttaa tggcgatacc gatggcctgt
     3421 gcaagatgag tcttacccag acctggtcct ccgtatatga agagtggatt gaaggtactt
     3481 ctgcccggat tgatggagat attcataccc gcagcacgtc caagccggtt gcactcacct
     3541 tcaatgaaat tggcgaatga gtattgtggg ttgagctgag gatcgatttt gagctttttc
     3601 agcccgggta taatataggg attcccatta tagttctgtc ccacctcggg tacgggaatc
     3661 tcgttgttat gataggtctt gctattttgc tccggaagac gtacctgttc cccttctacc
     3721 accgttaccg cataaatcag tgtgacctga ggtccaatct cacgacgtag cacaagattt
     3781 agcacatcta tataatgctc ctcaagatac tctctgaaaa aatgagaggg aacggcaagt
     3841 gtaatggttg aattctcgag tgacaccgac ctcacaggat gaaaccatgt gtcatattgc
     3901 tccttcggga taatgtcccg tataatcgcc agacacctgt tccatacatc tatgtgattg
     3961 gataaactca tttaaaaaaa atagggtaaa ttctactctt gtatttagcc gttagggctt
     4021 acaaagttgg tgaaaaaaaa agtaataaaa aaaactttta tctattgttt ttttgaaaac
     4081 taatacaact tattcattat caacgaaaaa aaaattaaaa aagcttaaat tactgtaata
     4141 cagattttta tcccatatta caattctatt acattgagaa tgatataatt ataatttaga
     4201 attattataa aatacgatta ttcttatcaa catggacgat gaaggctcct tcaaactttt
     4261 tccgaagcgc cggaagggct tttactgcct cctctcttgt agcaaatcta cccgtacagt
     4321 acttgtagta gccgttttga aatatttcga cacagttgtt gagacccttg aagtcaggtg
     4381 ctcccggctt aacctgtttt tttgaggcaa aaatctgaat catccaccaa tctgaagagg
     4441 tggattctga ggataccggc ttctcttttt cggaagaaag atcagtcgaa gcggaagaaa
     4501 cgggcttctc tttctccgag gaaacaccag tcgaaacaga ggggggatcg acctccatgc
     4561 cggagtggga atcataatgt ttcttgaatt cggaaaaggc agtgaagagt cttcctgcaa
     4621 tctccttgcg ccctgactcg gaagcgagct gtttacggtc tgctgcattt gagagaaatc
     4681 ctatttctac cagtaccgag ggcattgatg tcctccatag caccaacaga agggcctgtt
     4741 tgactcctct gtccaccgta ataggacctt tccggagttg ttcctggatt tttgatgcca
     4801 ttattacact ttgttcaaag tgcgcattct gcatcagatt gaagaagatg aaagattcgg
     4861 gttcgttggg atcatatccc tggtaggttg tagtataatc atcttcaagc agaatcacgg
     4921 agttttcctt cttgcagacc tccatattgg aatgactctc atccgtaccc attatatagg
     4981 tttcacttcc acgtggcttg gagcttggaa ccgaattaac atgaatggag ataaagaggt
     5041 cagcctggtt gcgattggca atattggttc tctgggccaa tgtaagatag gtatcttttg
     5101 aacgggtgta aaccacctta acatcaggat aggcatcctt gatgcgctgg ccgagcttaa
     5161 gtgcgacatc cagagtgatt gttttttccc ggattttgtt gtctgatgag atggctcccg
     5221 gatcttttcc gccgtgaccg gcgtcaatca ccactgtacg gagtttcata tccgagtttt
     5281 gtcccttaca aagcagctga ttcaagagta ccagtgtcac aaagaagagg aaaaacagtg
     5341 ctctatttgg tctgaatggc atactacttg ttaaaatttt actaattttg tcactttgca
     5401 aatatagaca aaaaatgcat ttttggtgcc aatgtcactg aacaaacaaa taacatacct
     5461 tctgtcgacc atattgctca tgatatgcgg gtgcataact ctgtttgccc aatcgcccga
     5521 ctcacttgtg caaaggcaga gaacattgtc cggagactcc gtggtggtac ttaagaatgc
     5581 tataagagat acgctgataa ctaccccacc ggactcctta actctgatgg atcttgagcg
     5641 tgatactacc ctcatatctc caccggattc tctttcaatc ttaatgactg aaaccgacac
     5701 t
//
LOCUS       DAOSIE010000078         5688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_12773_length_5688_cov_3.814131, whole genome shotgun sequence.
ACCESSION   DAOSIE010000078 DAOSIE010000000
VERSION     DAOSIE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12773_length_5688_cov_3.814131"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLI69_03470"
     CDS             complement(<1..871)
                     /locus_tag="PLI69_03470"
                     /inference="COORDINATES: protein motif:HMM:NF020418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimB/Mfa2 family fimbrial subunit"
                     /protein_id="HOG41581.1"
                     /translation="MKKILSILTVAALFVACNPIEPDGSKGEGSLTINVKGEIVTKAV
                     TDVVGNEATVNNLQIFIFDGDICKYYFDNGTTLSKTIDKVKTGTYEIWAVANLGETLS
                     ASISKTSLKAKALTLAQNAAASGFVMAGMNQVEVKASGTESVEITISRFVARARLVKV
                     TNSLSAGYGPVKIKYVMLDNVVSNQNIAGTAAITNWNNKMGRNSGTKIDGTTRSEAPE
                     SLLGNPTERNISAGNSDTPGFRFYGYANGTSDDSTDIVNWSARKTRLVVVAEIQGAIY
                     YYPVTLPSFARNSA"
     gene            complement(941..1945)
                     /locus_tag="PLI69_03475"
     CDS             complement(941..1945)
                     /locus_tag="PLI69_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41582.1"
                     /translation="MKRIATYMMIVVVLLPLLWACNDPIPCADCKMHRVVIHLNNTQP
                     APPLATRAIAETLEEERRINSVQLYIFGSEGNFLQRITGSGDTFEVITPAGECMFCCF
                     VNSPELPAAPSSPEELLGCDSYLWNNSRWSFQMVGTAIAKIDSDRELEIKVKRIVAKV
                     ECVIRTKFANSHLAGMPFTVHRIYLTNVAGKNNYALTAMQGNDGLWYNKMEYRQSSCD
                     ELICGTHTDRRMFDGDSLLIRETLYPYPNSNIDCFDRTLWSARKTRLVVEATLGEKLW
                     YYPITMEKTVANTHYLYDITITREGVEHPEMPITEPGSIETIVKIGKWNEGGEVDDEI
                     "
     gene            complement(1942..3912)
                     /locus_tag="PLI69_03480"
     CDS             complement(1942..3912)
                     /locus_tag="PLI69_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41583.1"
                     /translation="MKSKITILAAGVVMTLTIISCGSLSGRMESLGNYSASISLPQEN
                     RSALRELSIPEVMRDTSVVEDEKGNSYHLMRAVKDEKTGEMVATEELQAAVVTARFRN
                     VAERHGKVEIEFQVLVPKELQDSKWQLRMMPVMHIPEDTVELDRILITGENYRRSQLR
                     GYQQYRRFLNSIVTDSTKFMDVRQLEVFIKRNIPELYKYRTDSAYVSDELFLSYYGIS
                     AQEAINHYTYGLLVRHNEWKKANIGRMYDRYVKAPIVTEHLRLDTVMYNARGDFIYNY
                     VQRIQTRPHLRKVDIILEGEIYEQDNRIYTIEPSAPLTFYISSLSSFAEERERYLTQV
                     ISRRVKADDVCYIEFEKGREEVLGELSNNFQEIARIRRTLRVLLENRDFNLDSIVITA
                     SSSPEGSWEYNSGLSKRRSASVSRYFERFIKSWRDSSDREGGFSINMDGVLANSEMKR
                     ANVAVEFISRSIAEDWSTLNQLVMTDTVLTSLQKERYFLLSAEQNPDRRERLLSMEDS
                     YPYLNSTIYPRLRTVRFDFHLHRKDMVQDTIHTTIIDSTYMAGVEALRNRDYKLAVSL
                     LAPYRDINTAVAYCAADYNLSALDLLSELPPTPRVNYLMALLEARNGRSKSAAEYYLK
                     ACREDSHYIHRGNLDPEIYALVKMYDLNRYFE"
     gene            complement(3920..4483)
                     /locus_tag="PLI69_03485"
     CDS             complement(3920..4483)
                     /locus_tag="PLI69_03485"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HOG41584.1"
                     /translation="MVRLLLVVVIILSSALNVRAQRVAVSANLADIVNLGTLSGEISV
                     ATSRHISLNAGVRYNPWQYGSQEKGFVQNRKREASLGVRYWPWNIFSSWWFGMRTQVR
                     EYNLGGFFGRKVTEEGIAVGAEAAFGYSYMIHSRLNVEFGAGVWSGHKWYTRYDCPRC
                     GTIISEEKSHGWFLFPGEVIISLVYIF"
     gene            4625..5488
                     /locus_tag="PLI69_03490"
     CDS             4625..5488
                     /locus_tag="PLI69_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41585.1"
                     /translation="MIPIIKRESVAAVILMLVAMATQGCMEGADFVPENVELRFDWSS
                     FASEIFYEAQDHEVFYGNLEIRAPFKTALESAFFSKSTSESYRTEEGYHTYYGFTPRW
                     IPAVRVESQLRSYSRERIFYVTSLNPVMIGNIGLFRYPIAVRQRGTYGTYYLDEAQIA
                     ACIEDPGRWRDTTLIPVGEDQRFVRGRVELFGTLQRAMTAVGGSPLSGIAYTSPQPLQ
                     TMEVIISIGSLRMVLSAQYDAFYELFGKYDPSEELSVGDSVEINIPAVGVGHIPGLFL
                     VYPQSIRAIKE"
BASE COUNT         1451 a         1310 c         1259 g         1668 t
ORIGIN      
        1 acgcactgtt gcgtgcaaaa gaagggagtg taaccggata gtagtagatg gcaccctgta
       61 tttccgctac aaccaccagg cgtgtttttc gtgcagacca gttcacgatg tcagtggagt
      121 cgtcgcttgt cccatttgcg taaccataga aacgaaaacc gggagtatca ctgtttccgg
      181 cagaaatatt cctttcggta ggattgccga ggagtgattc gggtgcttcg gatctggtgg
      241 tgccgtcaat cttggtaccc gaattgcgcc ccatcttatt gttccagtta gttatggctg
      301 ccgtaccggc aatattctgg ttcgatacga cgttatcgag catgacatac ttgatcttta
      361 ccgggccata accggccgag aggctgtttg taacctttac aagtctggca cgcgccacaa
      421 aacgggaaat agtgatttcg accgattccg tgcctgaggc cttcacttca acttgattca
      481 tacctgccat cacaaaaccg ctcgcagcgg cattttgtgc cagtgtcagt gcctttgcct
      541 tgagtgatgt tttactgatt gaagcagaga gtgtctctcc cagattggct actgcccaga
      601 tttcgtaagt gcctgttttt accttgtcaa tggttttgga gagagttgtg ccgttgtcaa
      661 agtagtattt gcagatatcg ccgtcaaaaa tgaatatttg gaggttattg acagttgctt
      721 cattgccgac cacgtcggtg acggcttttg ttactatctc tccctttaca ttaattgtca
      781 ggcttccttc gcccttgcta ccatcgggct caatagggtt acaggccaca aaaagcgctg
      841 caacagttag aatggataga atttttttca tcattttgaa ttttttgata aagtttgtaa
      901 attgtgatta gttagttggt tgactgaaag agggttgcca ctatatttca tcatctactt
      961 ctccgccttc attccacttg ccgattttta cgatagtttc gatgctcccg ggctctgtaa
     1021 taggcatttc aggatgctca acaccttctc gtgttattgt tatatcataa aggtagtggg
     1081 tgttggctac cgtcttttcc atcgttatgg gatagtacca taatttctcg cccaatgtgg
     1141 cttcaaccac cagtctggtc tttcgtgcac tccagagagt acggtcaaaa caatctatgt
     1201 ttgaattagg gtaggggtaa agagtctcgc gaatcagtaa cgagtctccg tcaaacattc
     1261 gccggtctgt atgagtacca cagattaatt catcacagga actctgcctg tactccattt
     1321 tgttgtacca gagaccatca ttcccctgca tggcggtgag tgcataattg ttctttcccg
     1381 cgacgttggt caaatatatt ctatgcaccg taaatggcat tccggcgagg tgcgaattgg
     1441 caaatttcgt tcttatcaca cactctactt ttgctactat cctcttaacc ttaatttcaa
     1501 gctccctgtc ggagtcaatt tttgcaattg cagtgcctac catttgaaat gaccacctgc
     1561 tgttattcca gagatagcta tcacacccaa gcagttcttc tggtgaagaa ggtgctgccg
     1621 gcagttcggg agagttgacg aagcagcaaa acatacattc tcctgcggga gttattactt
     1681 caaaagtatc acctgagccg gttatccttt ggagaaagtt cccttcactg ccgaaaatgt
     1741 agagctgtac actgttgata cgcctctcct cttccaatgt ctctgcgata gcgcgtgtgg
     1801 ccaatggtgg tgcaggttgc gtgttgttta gatgaataac cactctatgc attttacaat
     1861 cagcgcaggg aataggatcg ttgcacgccc ataacaatgg cagcagcact acaactatca
     1921 tcatatatgt ggctattcgt ttcattcaaa gtatcggttt aagtcataca tcttgactaa
     1981 tgcatatatt tcgggatcga gatttcctct gtgtatgtag tggctatctt ctctgcaggc
     2041 ttttagatag tactcagcgg cactcttgct ccttccgttg cgcgcctcca gcaatgccat
     2101 cagatagttt acccgcggtg taggagggag ttcggagagg agatccagcg ccgataagtt
     2161 gtagtcggct gcgcagtagg cgactgccgt attgatgtcc ctgtagggtg caagcaagct
     2221 gactgccagt ttataatctc tattgcgcag agcctccacg cccgccatat atgtgctgtc
     2281 aataattgta gtatggatgg tatcctgtac catatcctta cggtgcagat ggaagtcaaa
     2341 cctcacagta cgcaacctgg gatagattgt gctattaaga tatgggtagc tatcttccat
     2401 tgagagcaat ctttcgcgcc tatcggggtt ttgttctgct gaaagcagga aatatctctc
     2461 cttctgcaag gaggttagca ccgtatctgt catcaccaat tgatttagag tactccagtc
     2521 ttcagcaatg cttcgcgaaa tgaattcaac ggcaacattg gcccttttca tctcactatt
     2581 cgctaaaact ccatccatat tgatggaaaa gccaccctca cgatctgaag agtctcgcca
     2641 gcttttgatg aatcgttcaa aataacggct tactgaggca cttctgcgct tgcttaatcc
     2701 ggaattgtac tcccagctcc cctcaggaga agaactcgcg gtgatcacga tggagtctaa
     2761 gttaaaatcc ctgttctcga ggagtacacg cagagtccta cgtatgcgcg ctatctcctg
     2821 aaaattattt gaaagctccc ccagtacctc ttcacgcccc ttttcaaatt ctatatagca
     2881 aacatcatcg gctttaaccc ttctggatat tacctgtgtc agatatctct ccctctcttc
     2941 ggcaaaactg ctgagagaag agatgtaaaa agtgagagga gcactgggtt cgatagtgta
     3001 gattctattg tcttgctcat atatctctcc ttccaatata atatcgactt tcctgaggtg
     3061 aggtctggtt tggatccgtt gtacgtagtt gtagataaaa tctcctcggg cattgtacat
     3121 gacggtatcg agacggaggt gttccgtcac aatgggagcc tttacatagc ggtcatacat
     3181 tctaccgatg ttggcctttt tccattcgtt gtgacgaaca agcagaccgt aagtatagtg
     3241 atttatggct tcctgtgcac ttatgccata ataggacaag aacagttcat ccgaaacata
     3301 tgcggaatcg gttcggtatt tatagagctc ggggatattt cttttgatga aaacctccag
     3361 ttgacgcacg tccataaatt ttgtgctgtc tgtgactatg gagttgagga atctgcgata
     3421 ttgctgatac ccgcgcaact gacttctacg atagttttcg cctgtgataa gaatacggtc
     3481 aagctccacc gtatcttccg gtatatgcat taccggcatc atcctgagct gccatttact
     3541 atcctgaagc tcttttggta cgagtacctg aaactcaatc tccactttcc catgccgttc
     3601 ggctacattg cgaaatcttg ccgttaccac agcagcctgc aactcttcag ttgccaccat
     3661 ctctccggtt ttctcatcct ttaccgccct cataaggtgg taggagttcc ctttctcatc
     3721 ttcgactacc gaggtgtcgc gcataacctc cggtattgag agttcccgta gagcgcttct
     3781 gttttcctga ggcagggaga ttgatgccga atagttgcca agcgactcca tcctcccgga
     3841 aaggcttcca caagagataa ttgtgagcgt cattacaact ccggcagcaa gtatggttat
     3901 tttacttttc atgctataat taaaaaatgt aaacaagtga tattatcact tctcccggaa
     3961 acagaaacca tccgtgtgat ttttcttcag aaataattgt cccgcagcgt gggcaatcgt
     4021 agcgagtata ccatttgtgt ccgctccaaa cccctgctcc gaactcgaca ttcaaccgac
     4081 tatggatcat gtaggaatat ccgaaagcag cctctgcacc tacagcaatc ccctcttccg
     4141 tgactttacg cccgaaaaat cctcccagat tatattcgcg tacctgtgta cgcataccaa
     4201 accaccatga ggagaaaata ttccatggcc agtatctcac tccgagcgaa gcttcgcgtt
     4261 ttctgttctg gacaaaacct ttttcctgag aaccatactg ccagggatta tatcttacac
     4321 cggcattgag agagatatgg cgtgaggtag ctacagagat ctcaccgctc aaagtaccca
     4381 gatttactat gtccgccaaa ttggctgaaa cagcaactcg ctgtgcccta acatttaaag
     4441 cggagctgag aataataact actaccaata acaacctgac catacccatc gcacattagt
     4501 tatttgactg caaaaatata taatatttat ataatcacaa aaataaataa ctaaaatata
     4561 gtagatattg acaaaataaa aataatgcta tatttgtact cataatattt aatacataaa
     4621 caatatgata cctataataa aaagagagtc tgtggctgcg gtgattttaa tgttggttgc
     4681 gatggcaacg cagggttgta tggaaggtgc agactttgtg cctgaaaatg ttgaattgag
     4741 attcgattgg tcctcatttg catcggaaat attttatgaa gcgcaagacc atgaagtttt
     4801 ttatggaaat ttggagataa gagctccctt caagaccgct ttagagagtg cattcttcag
     4861 taaaagcacg tcagagagct atcggacgga ggagggctac cacacctatt atgggtttac
     4921 tccccgctgg atacctgctg taagagtaga gtcacagctg cgttcctact caagggaacg
     4981 cattttctac gtaacatccc ttaacccggt gatgataggt aatataggcc tcttccgcta
     5041 ccctatagcg gtaaggcaga ggggcaccta tggtacctat taccttgatg aagctcagat
     5101 agctgcgtgc attgaagatc ccggacgatg gagagatact acgttaatac cggtaggaga
     5161 ggaccagcgc tttgtcagag gaagggtaga gttgtttgga acattgcaga gggcaatgac
     5221 tgccgtcgga ggctcaccgc tttcaggcat tgcatacacc tctccacagc cgctccagac
     5281 gatggaagtg ataatttcca taggctccct gcgtatggtc cttagtgcgc agtatgatgc
     5341 tttttatgag cttttcggca aatatgatcc ttctgaggag ttaagcgtgg gtgattctgt
     5401 ggaaataaat atcccggcgg tgggtgtggg acacataccc ggactctttc ttgtctatcc
     5461 gcagagcatc agagccatca aggagtgatt taattgttat ttttgcaata tggaaactat
     5521 tgcaatcatt atagcggcag gtgtggcgct gcttaccggt attattgttg aaatggagat
     5581 attaatggag acgattattg atttggatag aggtatttgt aaaaatccat tacccttctt
     5641 ataatcatct tctcgtgcgc tatatcctca tggatatatt cgacatca
//
LOCUS       DAOSIE010000079         5644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_12887_length_5644_cov_3.755949, whole genome shotgun sequence.
ACCESSION   DAOSIE010000079 DAOSIE010000000
VERSION     DAOSIE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12887_length_5644_cov_3.755949"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PLI69_03495"
     CDS             complement(<1..700)
                     /locus_tag="PLI69_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG41586.1"
                     /translation="MSKVDMERKEKVKKSAASGLIALIFLIIGFQTAIFFEKVINRPA
                     PQTEFEEVKDVARHHSGTDDSLRQPSSYSSHEFSSGTSYRSDNAIKNKRAKEYGGKTR
                     FGGYSAPPTSYRKPVRAPRIVESFPFDPNTVTAEELVRLGLSERQAEVILNYRNKGGS
                     FRRKEDFARMYVVSDTLYQRLEPFIDIPKLELNKADSAALVTLKGIGPYYARRIIEYR
                     ERLGGFFDPQQLMEI"
     gene            complement(731..1429)
                     /gene="rsmI"
                     /locus_tag="PLI69_03500"
     CDS             complement(731..1429)
                     /gene="rsmI"
                     /locus_tag="PLI69_03500"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOG41587.1"
                     /translation="MSRLYIVPTPIGNLEDITLRAIRILKEVDLILAEDTRTSSVLLR
                     KYGISTRMESHHKFNEHRTVASLTEKILSGVEVALISDAGTPGISDPGFLLVRSCVEA
                     GIEVETLPGATAFVPALVNSGFPCDRFVFEGFLPVKKGRQTRLKALQEENRTMIFYES
                     PHRLLKTLTQFAEYFGSERPASVSREISKIHDTTHRGTLASLLEYFNSHEPKGEIVIV
                     VSAHHPTKNQEQDS"
     gene            1473..2042
                     /locus_tag="PLI69_03505"
     CDS             1473..2042
                     /locus_tag="PLI69_03505"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOG41588.1"
                     /translation="MFIESAKKPGSIEVICGSMFSGKTEELIRRLKRAKFANQNVEIF
                     KPKIDIRYSDAEVVSHDSNTILSTPVDSSNNILLLTGNVDVVGIDEAQFFDHGIVDVA
                     QQLAAQGIRVIIAGLDMDYLGNPFGPMPALMAVAEYVTKVHAICVRCGDIANHSHRLS
                     SSDKLVELGEKDIYEPICRHCFNKLKKAT"
     gene            complement(2152..>5644)
                     /gene="rpoC"
                     /locus_tag="PLI69_03510"
     CDS             complement(2152..>5644)
                     /gene="rpoC"
                     /locus_tag="PLI69_03510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOG41589.1"
                     /translation="PLDGGRFATSDINDLYRRVIIRNNRLKRLIEIKAPEVILRNEKR
                     MLQEAVDSLFDNSRKSNAVKTEANRTLKSLSDSLEGKQGRFRQNLLGKRVDYSARSVI
                     VVGPELKMHECGLPKLMAAELYKPFIIRKLIERGKVKTVKSARKLVDRKDSEIWEILE
                     NVMKGHPVLLNRAPTLHRLGIQAFQPKMIEGKAIQLHPLACTAFNADFDGDQMAVHLP
                     LGNAAILEAQLLMLGSHNILNPANGAPITVPSQDMVLGLYYITKEREGAKGEGMKFYG
                     PEEAIIAYNEKIVELQSKVKVRLPIDRANPEKGYHMVDTTVGKILFNQLVPAEVGYIN
                     ELLKKKSLRDIIGKVLKTSDVARTAQFLDDIKDLGYKMAFQGGLSFNLGDVIVPKEKQ
                     RLVDAGYDEVESIQASFNMGLLTNNERYNQIIDVWTKTNNNLTRIVEEQIRKDNQGFN
                     PVFMMLDSGARGSKEQIRQLCGMRGLMAKPQKSGATSAEIIENPILANFKEGLSVLEY
                     FISTHGARKGLADTALKTADAGYLTRRLVDVSHDVIINEEDCGTLRGLIATAIKNKDE
                     IVETLNERILGRTSVHDIYNPLTAEKILSAGEEITEEIADLIDTLPIESVEIRSVLTC
                     ESRKGVCAKCYGRNLATGRMVEKGEVVGVIAAQSIGEPGTQLTLRTFHVGGTAGNITS
                     ESEIISKYEGKLEFEDLRTIRKVDSKGERTIVVSRATEMKIVHDTTGINLSQYTIPYG
                     AELYKAPGDLVEKGDRICSWDAYNSLNISEYSGTVVYENLINGVTYREERDETTGHIE
                     RVVIESRDKSKTPSVKIVDEDGTIIQTYTLPVNAHIMVENKQPIVAGDIIHKIPRAIG
                     KSGDITGGLPRVTELFEARNPSNPATVSEIDGEVSLGKIKRGNKEVIITPKNGEKKTY
                     LVPLSKQILVNDNDHVKAGTPLSDGVISSSDILAIQGPVKVQEYIVNEIQEVYRMQGV
                     KINDKHFEIIVRQMMRKVEIVEPGDTNFLPEQLVDKWEFMEENDEIFDKKVVTDAGDS
                     TELHKGEIVTLRKLRDENSILKRQDKRPVIARDATPATSKQVLQGITRAALRTNSFMS
                     AASFQETTKVLSDAAIRGKQDTLEGLKENVICGHLIPAGTGQRSFEKLVVGNMEDVQK
                     ALNISRPRRRERPAERE"
BASE COUNT         1455 a         1355 c         1214 g         1620 t
ORIGIN      
        1 ctatctccat aagttgctga ggatcaaaaa aaccgcccag acgctccctg tattcgatta
       61 ttctacgcgc atagtagggt cctatgcctt taagagtgac taaggcagca ctatcggctt
      121 tattaagttc caacttcggt atatcgataa aaggctcaag gcgctgataa agggtatccg
      181 acactacata cattctggca aagtcctctt tcctacgaaa tgaaccacct ttatttctgt
      241 aatttagtat tacctcagcc tgacgttcgg agagtccgag tcgtacgagt tcttccgcag
      301 ttaccgtatt gggatcgaat ggaaatgatt ccactattcg tggagctctg acaggctttc
      361 tgtaactagt cggaggggca gagtaacctc cgaaacgggt ttttccgcca tactctttag
      421 ctcttttgtt tttgattgca ttgtcgcttc ggtaagatgt tccggaagaa aactcatgtg
      481 aggagtacga tgatggctgc cgtagggaat catctgtccc ggaatggtgg cgcgccacat
      541 ctttcacctc ttcaaattcc gtttgaggag ccggtctgtt gatcaccttt tcaaagaaaa
      601 tcgccgtctg aaatccaata ataagaaata ttagcgctat cagacccgat gcagcgctct
      661 tttttacttt ttcctttctc tccatatcta ccttgctcat taaaattttt gttaaaaatg
      721 taaccggatg tcagctatcc tgttcttgat ttttagtcgg atggtgcgca gagaccacaa
      781 tcactatttc acctttaggt tcatgcgaat taaaatattc cagaagagat gcgagagtgc
      841 ctctatgggt agtgtcgtga attttagaaa tttctctcga tacacttgcc ggacgctcag
      901 aaccgaaata ctccgcaaac tgcgttaacg tcttcaataa acgatgcggc gattcgtaga
      961 atatcatagt tctattctcc tcctgaagtg ccttaaggcg tgtttgtcgc ccctttttta
     1021 ccggaaggaa tccttcaaat acaaatctgt cacaggggaa acccgagtta accagcgccg
     1081 gaacaaatgc agtagctccg ggaagcgttt caacttctat accggcctct acacaacttc
     1141 ttaccaagag gaaaccgggg tctgatattc cgggagtacc ggcgtcactg atcaaagcca
     1201 cctctacccc tgaaaggatc ttctccgtta atgaagcgac agtcctgtgc tcgttaaact
     1261 tgtgatgtga ctccatacgg gtggaaatgc catatttccg caacaaaacc gaacttgtcc
     1321 tggtatcttc tgccaaaatc agatccacct ccttgagaat tctaatggct ctcaaggtaa
     1381 tatcttcaag attaccaatt ggcgtaggga ctatgtaaag tcttgacatt tttgtttacc
     1441 tttgtatgga gttccatgca aaattaagaa aaatgtttat agaaagcgca aaaaaaccgg
     1501 gctcaataga agtcatctgt gggtcaatgt tttcaggtaa gactgaggaa ttgattcgtc
     1561 gccttaagag agcaaaattt gcaaatcaga atgttgagat atttaaacct aaaatagata
     1621 tcagatattc cgatgcagag gtggtttccc atgactctaa taccatactt tcaacgccgg
     1681 tcgattcatc caacaacatt ttattgctaa caggcaatgt ggatgtggta ggcattgacg
     1741 aggctcagtt tttcgaccat gggatagtag atgtcgcaca acaactggct gcacagggca
     1801 tcagagtgat aatagcggga cttgacatgg attatctcgg caatccgttt ggccctatgc
     1861 ctgctctgat ggctgtagcc gaatatgtca ccaaagtaca cgcaatctgt gtaagatgtg
     1921 gcgacattgc aaaccactca catcgtctct cttccagcga taaacttgtc gaactcggag
     1981 aaaaagatat ttacgaaccc atttgccgtc actgtttcaa caaactcaaa aaagcgactt
     2041 aagtcgcttt ttagttctgt gttccgagta tttcgttccg agtaactaca cactacatac
     2101 tcacaactcc aaattaaaaa cggcctcaaa taagaggccg tttttaattt gttactccct
     2161 ttccgccggt ctttcacgcc tgcggggtct cgaaatatta agtgccttct gaacatcttc
     2221 catattgcct acgaccagtt tctcaaatga acgttggccg gtaccggcag gaataagatg
     2281 accacagatc acattttcct taagaccttc cagagtatcc tgcttgccac gtattgcagc
     2341 atcgctcaac accttggtgg tctcctggaa tgatgcggcc gacataaagc tgtttgtcct
     2401 caaagcagca cgggtaatac cctgaagtac ctgttttgag gtagccggag tagcatcacg
     2461 cgcaataacc ggcctcttgt cttgacgttt caggatcgaa ttctcatcac gaagtttgcg
     2521 aagagtaaca atctctcctt tgtgcagttc ggtagaatca ccggcatctg tgacaacctt
     2581 tttgtcgaag atttcgtcat tctcctccat gaattcccat ttatcaacaa gctgttcagg
     2641 gaggaaattg gtgtcaccgg gttcaacgat ttctacttta cgcatcatct gacgcacaat
     2701 tatctcgaaa tgcttgtcgt tgatcttcac accctgcata cggtaaactt cctgaatctc
     2761 attcactata tactcttgta cttttacagg accctggatg gcaagaatgt cggaagagga
     2821 gattactcca tccgagagag gagtaccggc cttaacgtga tcattgtcat ttacaagaat
     2881 ctgcttggaa aggggtacaa gataggtctt cttctcacca ttcttagggg tgatgataac
     2941 ctccttattt cctcttttta tcttgccaag cgaaacttca ccgtcaatct ctgagacagt
     3001 agccggattg gaaggatttc tggcttcaaa tagctctgtt acacgaggaa gaccacctgt
     3061 aatatcacct gacttaccaa ttgccctagg tatcttgtgt atgatatcac cagccacaat
     3121 cggctgtttg ttctctacca taatgtgggc attaaccggt agagtatagg tctggattat
     3181 ggtaccgtct tcatcgacaa tcttgacaga aggagtcttc gacttgtcac gagactcaat
     3241 aacaactctc tcaatgtgtc ctgtagtctc atctctctct tcacgatagg taactccgtt
     3301 gatgagattc tcataaacca cggttccgct atattcggaa atattgagtg aattatatgc
     3361 gtcccaacta caaatcctgt cacctttttc tacaaggtca ccgggagctt tgtagagttc
     3421 cgctccatag gggatagtat attgtgagag gttgataccc gtggtgtcat gcacgatttt
     3481 catctcagtt gcacggctca ccactatggt acgctcacct ttactgtcaa cttttctaat
     3541 ggttctaaga tcctcgaatt caagttttcc ttcatacttg gagataattt cactttcgga
     3601 agtgatattt cccgcagtac caccgacgtg gaatgtacgc agagtaagct gtgtaccggg
     3661 ttcaccgata gactgtgctg ctatcacacc tacaacttca cctttctcta ccatgcgacc
     3721 ggttgccagg ttacggccat aacacttggc acatactccc ttgcgtgact cacaggtaag
     3781 cactgaacgg atctcgacac tttcgatagg aagcgtatcg ataagatccg caatctcttc
     3841 ggtaatttct tcacctgcag aaagaatttt ttcagcggtc agcgggttgt aaatatcatg
     3901 tactgaagtg cgaccgagaa ttctctcatt gagagtctcg acaatctcat ccttgttctt
     3961 gatagcagta gctataagtc ctctgagcgt accgcaatcc tcctcgttga taataacatc
     4021 gtgagaaacg tccaccagac gacgtgtcag ataacctgcg tccgccgtct taagtgcggt
     4081 atccgcaaga cctttacgag caccgtgggt ggagatgaag tattccagta ccgaaaggcc
     4141 ctccttgaag tttgcaagga tggggttctc aatgatctcc gcactggttg cgccggactt
     4201 ctgaggttta gccataagac cacgcatacc acaaagctga cgaatctgct ctttggaacc
     4261 acgcgcacct gagtcaagca tcataaatac aggattgaat ccctgattgt ctttgcgtat
     4321 ttgttcctca acgattcttg tcagattgtt gttggtcttg gtccaaacgt caataatctg
     4381 gttatagcgc tcattgttgg taagaagacc catattgaaa ctagcctgta tgctctccac
     4441 ctcatcataa cctgcatcca cgagtcgctg tttctctttg ggtacgataa cgtctccgag
     4501 gttgaatgaa agacctccct ggaatgccat cttgtaacca agatccttga tatcatccag
     4561 gaactgcgcg gtccgggcca catctgatgt cttgagcacc ttaccgataa tatcacgtag
     4621 tgattttttc ttcaggagtt cattgatata ccctacctct gcagggacga gctggttgaa
     4681 gagaatctta cctacggtgg tgtccaccat gtgataccct ttttcaggat ttgcacgatc
     4741 tatgggaagg cgtaccttaa ccttggactg gagctccact atcttttcgt tataagcgat
     4801 tatggcctct tccgggccat agaacttcat accctcaccc tttgcgccct ctctctcctt
     4861 agttatgtag taaagcccta gaaccatgtc ctgtgaaggc acggtgatgg gagcaccatt
     4921 tgcaggattg agaatattgt gcgagccaag catcaatagc tgagcctcga gaattgcagc
     4981 gttgccaaga ggtaggtgaa ctgccatctg gtctccgtca aagtcggcat taaaggcagt
     5041 acatgcgagc ggatgaagtt ggatggcttt accctcgatc atcttaggct ggaaggcctg
     5101 tataccgaga cggtgaagtg ttggtgcacg gttaaggagt acagggtgac ccttcatgac
     5161 attttccagg atttcccaaa tctccgagtc cttcctgtca acaagttttc tcgcggactt
     5221 tacggtcttt accttacccc tctcaataag cttacgaatt ataaaaggtt tgtagagttc
     5281 agccgccatg agtttcggaa gaccgcactc atgcatcttg agttcaggac caacaacgat
     5341 aaccgagcgg gcagaatagt caacgcgctt ccccagaaga ttttgacgga agcgtccctg
     5401 cttaccttcg aggctatctg aaagtgattt cagagtcctg ttggcctctg tttttacagc
     5461 attggactta cgcgagttgt caaagaggga gtcaaccgcc tcctggagca tacgcttctc
     5521 gttgcgtaga attacctcag gagctttgat ctcaatcagt ctctttagtc ggttgttacg
     5581 aattattacc ctgcggtaaa ggtcattgat atcagatgtt gcaaaacggc ctccatcgag
     5641 agga
//
LOCUS       DAOSIE010000080         5638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_12906_length_5638_cov_3.436862, whole genome shotgun sequence.
ACCESSION   DAOSIE010000080 DAOSIE010000000
VERSION     DAOSIE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12906_length_5638_cov_3.436862"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..2047
                     /locus_tag="PLI69_03515"
     CDS             113..2047
                     /locus_tag="PLI69_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOG41590.1"
                     /translation="MEQTDKLNNYTYDDDSIVTLEDLEHIRLRPGMYIGRLGDGTEPN
                     DGMYVLMKEVIDNSIDEFAMGHGKQITMKIEDKKIHIRDYGRGIPFGFSEKDKNVNQL
                     DAAVTKLHTGAKFDDKTFQKSVGMNGVGLKAVNALSSHFTVQSFRSGETLALEYEKGI
                     LTTPNFNPVPTTEKNGTLVTFIPDDTLFEPYEYNLEYIDTMLRNYSYLNVGLKLSFNG
                     TDYKSDNGLLDLLNDSMVEEPLYPPIHLKNGDIEVVFTHGNDYGENIHSFVNGQNTTL
                     GGTHLSAFKEALTKTLKEYYKKDFNPVDIRQSIIGAVSVRIQEPEFEGQTKVQLGSKY
                     MWQNKKDGTHGPTITKTMSDFLMTELDNYLHKNLDVADIILKKIQESEQERTAISKVS
                     RKTRERLKKANIHNKKLRDCRIHYNDKTSKGEMHPLAKETTIFITEGDSASGSITSSR
                     DANTQAVFSLKGKPFNCAKASKKDLYVDRNEELCLLQSALDIEEDIENLRYNNVVIAT
                     DADVDGMHIRLLLLTFFLKFHPDLVRMGHLYILKTPLFRVTDKKTNIYCYNSAERDKA
                     ISKLGKKAEVTRFKGLGEISPEEFKGFIGPEMRLEAVRLTKEENINDIIEFYMGTNTP
                     IRQKFIRNNLRSDINNSEEV"
     gene            2050..2613
                     /locus_tag="PLI69_03520"
     CDS             2050..2613
                     /locus_tag="PLI69_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG41591.1"
                     /translation="MWRKFCKFLLKSLGWKVAHDAPVPEDKCIILGVPHTSIWDFVIS
                     YLYYQSIGGEAKCMIKKEMFVWPLGWLLKKLGSVPIDRKSGAAVTKQMIGIFNSSEKF
                     HLALAPEGTRKPVKRWKLGFHTIAKTTGVPVYLGYFDWGRKIIGHGERFELTDDAMAD
                     LREIQKIYKAMNLKGRYDGRFDFLEGV"
     gene            complement(2610..3590)
                     /locus_tag="PLI69_03525"
     CDS             complement(2610..3590)
                     /locus_tag="PLI69_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41592.1"
                     /translation="MSKINGRTIALLILALVLYVSAFFTASEPMDFEAVLPFGSPKFL
                     NALFGATTLSYILSVVFLILTAVSIYSFNMMYTTGINYLLPVLYIFLALYNQRVIFFS
                     PLHIVALLLIWSLAFSAKYRDAVQNLSDNYLSFFLFGTASLFFPPLVWIFPLITLVNY
                     RASEEKFKYVFTSLLGLFTPLVMVSAICYIFGGKELLSSLGASFWSSMSTLGRYGIDF
                     TTVEIVALLVIITFALISTFHVLRNLNRYKIIHSNTSIRIIFFAILITLLFAVFITDN
                     HDPYGVVLFVPVSLILFEYLSSPKNNKGAMIFYFMVSGLAIISRIIVFVR"
     gene            complement(3612..4892)
                     /gene="purD"
                     /locus_tag="PLI69_03530"
     CDS             complement(3612..4892)
                     /gene="purD"
                     /locus_tag="PLI69_03530"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOG41593.1"
                     /translation="MNVLVLGSGGRESALAWKIAGSPLLEHLYCMPGNPSTASFATNV
                     DCSLSDFESIARHISDLSVDMLVVGPENPLVEGITDFFRERIPSLMVIGPGRDGARLE
                     GSKDFAKAFMSGHSIPTAAYRTFEASQIEEASAFLEEIKPPYVLKADGLAAGKGVIIT
                     HSLEEARQELASMFGGRFGDAGAKVVIEEYLSGIELSVFVLTDGKDYLMLPHAKDYKR
                     IGEGDTGPNTGGMGSVSPVPFADESFMAKVEERIIRPTIRGILSEGMNYCGFIFIGLM
                     NCGGDPYVIEYNVRMGDPECESVVRRIDSDLLEHLRAAATGTLSLEKIEVSPMSSVTV
                     MAVSGGYPGSYKKGFTISGLDVNYGEEDTVIFHAGTAVSGNNVVTSGGRVLAVTSLAA
                     SLDEARKASYGRLAKIGFEGIFYRSDIGCDLLDF"
     gene            4969..5574
                     /locus_tag="PLI69_03535"
     CDS             4969..5574
                     /locus_tag="PLI69_03535"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOG41594.1"
                     /translation="MKRSGILILSLLLTALSCGPRADIPKKQMIEIYYEISLLDSYVD
                     HNPEMRAQSDSMKVYAPLLDKYGYTEEEFINSINFYLGDPVRFLKIIEAVETKLKERE
                     QYLSDLQRADGIEDERMIEEAVAFDEGAPVEEYPAFEASDTFSQENSAWESHVKDTLP
                     KDTIVRELPDTKNRRITTREKRVTREELIELSKKFKEQKSK"
BASE COUNT         1809 a         1264 c         1221 g         1344 t
ORIGIN      
        1 tattgttgta aatatgatct ttaataatcc tgcaaaaata accttttctt ctcaataaag
       61 gcacaaaaag tgctatattt gcgctcagat tgttaaaatg acggaacgat agatggaaca
      121 aacagataaa ttgaacaact acacctacga cgatgatagc atagtcactc tggaagatct
      181 ggagcatatc aggctgagac ccggaatgta tatcggacgc ctcggcgatg gcaccgaacc
      241 caacgatgga atgtacgtgc tgatgaaaga ggtgattgat aactctattg atgaatttgc
      301 catggggcat ggcaaacaaa tcactatgaa gatcgaggat aaaaaaatcc acatcagaga
      361 ctatggacgc ggcataccct ttggcttcag tgaaaaggac aagaatgtca accagctgga
      421 cgcggcagtg actaaacttc acacgggagc aaagtttgat gacaagacct tccagaaatc
      481 ggtaggtatg aatggtgtcg gccttaaagc tgttaacgct ctttcttcac attttaccgt
      541 acaatctttc cgttccgggg agacacttgc acttgaatac gaaaagggta tacttacaac
      601 tcccaacttc aatccggtgc cgacaacgga gaaaaacggc actcttgtaa ccttcattcc
      661 cgacgatact ctctttgaac cctacgagta caatctggag tatatcgata cgatgctccg
      721 caactactca tacctcaacg taggtctcaa actcagtttc aacggtaccg attacaagtc
      781 tgataacggt ctgctggatc tgcttaacga ctccatggtg gaagaaccac tctacccacc
      841 tatacacctt aaaaatgggg atatagaagt agtctttact cacggcaacg attatggaga
      901 gaatattcac tcctttgtca acggacaaaa cacaactctt ggcggtacgc atctctcagc
      961 atttaaggaa gcactcacta agaccctcaa agagtattat aaaaaggatt tcaatccggt
     1021 ggatatccgc cagtcaataa taggggcagt cagcgtacgc atacaagaac cggaatttga
     1081 gggacagacc aaggtgcaac tgggctccaa atatatgtgg cagaataaaa aagatggcac
     1141 ccacggtcct accataacca agacgatgag tgattttctt atgactgaat tggataacta
     1201 tctccacaaa aatctcgatg tagcagatat tatcctaaag aagattcagg agtctgagca
     1261 ggagcggaca gccatctcca aagtgagcag aaagaccagg gaacgtctta aaaaagccaa
     1321 tatccacaac aaaaaactga gagactgccg tatccactac aacgataaga cttccaaagg
     1381 tgagatgcat cctctggcaa aggaaactac aatattcatc accgagggag actcggcatc
     1441 aggctcaatc acatccagca gggatgccaa cacacaggcc gtattcagtc tcaagggaaa
     1501 accctttaat tgtgccaagg cctccaaaaa ggatctctac gtagatcgca atgaggaact
     1561 ctgcttattg cagtcggcac ttgacattga agaggatatc gaaaacctgc gttacaacaa
     1621 tgtggtaatt gccaccgacg ccgacgtaga tggaatgcat attcgactgc tgctactcac
     1681 ctttttcctg aaattccacc ctgaccttgt gcgtatgggg cacctctata tactcaaaac
     1741 cccattattc agggtaactg acaagaaaac caacatctat tgctacaatt ctgcggaaag
     1801 ggataaggct atcagcaaac ttggtaaaaa ggcggaagta acccgtttca agggacttgg
     1861 agagatttct cccgaagagt ttaagggctt tataggaccg gaaatgcgcc tggaagctgt
     1921 acgcctgaca aaagaagaga acatcaacga cataatagag ttttatatgg gtaccaatac
     1981 acccataaga caaaaattca tcaggaacaa tctccgttcc gatataaata atagcgagga
     2041 ggtttaacaa tgtggcgcaa attctgtaaa tttcttttaa aaagtctggg gtggaaagtt
     2101 gctcatgacg ctcccgtacc tgaagataaa tgcattattt tgggtgtacc tcatacatct
     2161 atttgggatt ttgtcatctc ctacctctat taccagtcaa ttggagggga agcaaaatgc
     2221 atgatcaaga aagagatgtt cgtttggcct ttgggctggc tgctaaaaaa actcggttcg
     2281 gtacccattg accgcaaaag tggggcagcc gtcaccaaac agatgatcgg gattttcaat
     2341 tccagcgaaa aatttcactt ggcactcgct cctgaaggga cacggaaacc ggtaaaaagg
     2401 tggaaactcg gattccatac tattgctaaa acgaccggag tccctgtcta tctgggctat
     2461 tttgattggg gacgcaagat aataggacat ggagaacgat ttgaactgac cgacgatgct
     2521 atggcagatc tgcgtgaaat tcagaaaatt tacaaggcta tgaatctcaa agggcgttac
     2581 gacggtcgct tcgacttttt agagggagtc tagcgtacaa aaacaataat tctggatatt
     2641 attgcaagac cggagaccat gaagtagaaa atcatggctc ctttgttgtt tttaggagag
     2701 gagagatatt caaaaagaat cagtgataca ggcacaaaca gcactacccc gtatgggtcg
     2761 tgattatccg taataaagac ggcaaagagc aacgtaatca gtattgcaaa aaagatgatg
     2821 cggatagagg tattggagtg gatgatcttg taacggttaa gattgcgcaa cacgtgaaat
     2881 gttgaaatca aagcaaaagt aattattaca agaagtgcaa ctatttccac ggtagtaaag
     2941 tcgataccat atctgccaag agtactcatc gaactccaga aagaggctcc caaactactc
     3001 agaagttcct ttccgccaaa aatataacat attgcactaa ccatcaccaa gggtgtgaaa
     3061 agtccaagta atgaggtaaa aacatacttg aacttctcct ccgaggctct ataattgaca
     3121 agcgtgatca aaggaaaaat ccacaccaaa ggtggaaaga aaagagatgc agttccaaaa
     3181 aggaaaaatg aaagatagtt gtcactcaaa ttctggacgg catcgcggta tttagcggag
     3241 aaggccagcg accagatcag cagtaacgcc actatatgta gtggagagaa gaatatgact
     3301 ctctgattgt agagggcaag gaagatatac aacaccggta gcaggtagtt gatacctgta
     3361 gtatacatca tattgaaaga atagatgctg acggctgtaa gtataagaaa aactacagaa
     3421 agaatatatg agagggtggt agcaccgaat aatgcattga gaaacttcgg agagccaaaa
     3481 ggcaatactg cctcaaaatc cattggctca gaagcggtaa aaaaggcact cacatagagt
     3541 acaagtgcca aaatcagcag cgcaattgtc cttccgttga tctttgacat acttactcca
     3601 ccctcccatt atcagaaatc caacaaatcg cagcctatat cgctgcggta aaagatgcct
     3661 tcaaacccaa tttttgcgag ccttccatag gatgcttttc tcgcttcatc caaagaggcc
     3721 gccagcgatg ttactgccag tacccttcca ccggacgtta ccacattatt gcctgaaacc
     3781 gcagtaccgg catggaatat gacggtatcc tcttccccgt agttgacatc gagacccgaa
     3841 atcgtaaatc ccttcttata actaccggga taaccaccgg ataccgccat caccgtgacg
     3901 gaactcatcg gtgagacctc tattttttcc aatgagagag tacctgttgc agcagcccgg
     3961 aggtgttcca gaagatcgct gtctatgcgc cttactaccg attcacactc aggatctccc
     4021 atacggacat tatattctat aacataaggg tccccgccgc aattcatcag gccgatgaat
     4081 atgaagccgc agtagttcat accctcggat agtatacctc gtatggtggg ccttattatg
     4141 cgctcttcaa cttttgccat gaaactttca tcagcaaaag ggaccgggga taccgaaccc
     4201 attccacctg tattcggacc tgtatcaccc tctccaatac gtttgtaatc cttggcatgg
     4261 ggaagcatca gataatcctt tccatcggtc agcacaaaaa ccgagagctc gataccggag
     4321 agatactcct ctatcaccac ttttgcaccg gcatcgccga aacgacctcc aaacatcgaa
     4381 gcaagctcct gacgggcctc ctccaaagaa tgagtgatga taactccctt acccgctgca
     4441 agaccgtcag cttttaatac atacggaggc tttatctctt ccagaaaagc cgaagcttct
     4501 tcaatctgag aggcctcgaa agtcctgtaa gcggcagtag ggatagagtg tccggacatg
     4561 aaagccttgg caaaatcctt actcccttca agccttgctc cgtcacgacc ggggcctata
     4621 accataagtg aaggtattct ctcacgaaaa aaatcggtaa taccctctac aagcggattt
     4681 tcaggaccta cgaccaacat atctacggaa agatcggaaa tatggcgtgc aatggattca
     4741 aagtccgaaa gactacaatc cacatttgtt gcaaaagatg ctgtactggg attacccggc
     4801 atacagtaaa gatgctctaa aagaggacta ccggctattt tccaggctaa agcactttct
     4861 ctcccgcccg aacccaatac aaggacattc atataggttg aaactaattt tgacaaagat
     4921 aggtataaaa attgctatat ttggagttta aatggcaaat cttctacgat gaaacgttca
     4981 ggcatactca tactctctct cttgcttact gctctatctt gtggtcccag agcggatatc
     5041 cctaaaaaac agatgataga gatatattat gagatctctc ttctcgacag ctatgttgac
     5101 cataatccgg aaatgagggc acagagtgat tcaatgaagg tttatgctcc tttacttgac
     5161 aagtatggct atactgaaga ggagttcatc aattccatca atttttatct aggggatccc
     5221 gtgcgattcc taaagattat cgaagcagta gaaaccaagc ttaaggagcg cgagcaatac
     5281 ctctccgatc tgcagcgagc tgatggaatt gaggatgaaa ggatgataga agaggcagtt
     5341 gcttttgatg agggtgcgcc ggtagaagag tatccggcct ttgaagcatc tgatactttt
     5401 tctcaagaga actcagcctg ggagagccat gtcaaggata ctttgccgaa ggacaccata
     5461 gttcgagaac tacccgatac gaaaaatcgt cggattacta ctcgcgaaaa gcgtgtgacc
     5521 agagaggaac tcattgaact gagcaaaaaa tttaaggaac agaaaagcaa atgatatata
     5581 tagccgacca tattttcccc attgagggag aacccatcat aaacggatat gtagagat
//
LOCUS       DAOSIE010000081         5590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_13027_length_5590_cov_3.870641, whole genome shotgun sequence.
ACCESSION   DAOSIE010000081 DAOSIE010000000
VERSION     DAOSIE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13027_length_5590_cov_3.870641"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLI69_03540"
     CDS             complement(<1..211)
                     /locus_tag="PLI69_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG41595.1"
                     /translation="MYLKSIILHNFKNIVAASLEFSPKINYISGNNGAGKTNLLDAVY
                     YLSMTKSFFSSSDRYVCNFDAEEAQI"
     gene            358..1038
                     /locus_tag="PLI69_03545"
     CDS             358..1038
                     /locus_tag="PLI69_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG41596.1"
                     /translation="MATKKKHSTQEEVKLGTAISSIELFFQDKKNQQRLFIVTSAIIV
                     IVFGIIAINRWYLQPLKAEARAQMFPAEQLFMDGDYATALNGDGNVMGFAQIANQYGN
                     KAGKIIYFYKGICQLQTGDNEGAIKSLKKYDGKDKIAKARAYCCIGDAYVNLDKNTEA
                     LKWYKKAIAEESHAYTAGYLLKAGIISEEMGDNEQALKFYDEISIKYPNSIEAYEVGK
                     YISRLKNK"
     gene            1043..1771
                     /locus_tag="PLI69_03550"
     CDS             1043..1771
                     /locus_tag="PLI69_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG41597.1"
                     /translation="MGRAFEFRKERKFKRWGNMARVFTRLGKEITIAAKQGGTDPAGN
                     PHLRTLIQTAKSENMPKDNIERAINRAIEKDQSDYKEVVYEGYGPHGVAFLVETATDN
                     TNRTVANIRSYFAKYGGSLGTSGSVAFMFDHKCAFKIKSEAPVDLEELELELIDYGAD
                     EVFKNDDDDIINIYGEFTAFNNIQKFIEEKGFEIVSGGFERFPNGELKKVSAEERVEL
                     NKLIEKLEEDEDVQNVYHTLEIEE"
     gene            1911..2318
                     /gene="mce"
                     /locus_tag="PLI69_03555"
     CDS             1911..2318
                     /gene="mce"
                     /locus_tag="PLI69_03555"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOG41598.1"
                     /translation="MDLTHIEHIGIAVKSLEEAIPYYEDVLGLKCYKIEEVADQKVKT
                     AFFRIGDTKIELLESTSPEGPVAQFIEKRGEGVHHIAFATSSVKDCLAKAEAKGLRLI
                     DKTPRRGAEGLNIAFLHPKSTMGVLTEFCEKGE"
     gene            2349..3911
                     /locus_tag="PLI69_03560"
     CDS             2349..3911
                     /locus_tag="PLI69_03560"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOG41599.1"
                     /translation="MSHQLEKVKELIELREKARMGGGQKAIDAQHAKGKYTARERISL
                     LLDEGSFEEFDMFMTHRCTNFGMEKKTFLGDGVVTGYGTIDGRLVYVFAQDFTVIGGS
                     LSETLALRICKVMDQAMKVGAPIIGLNDSGGARIQEGVNALAGYAEIFQRNILASGVV
                     PQISAILGPCAGGAVYSPALTDFNLMVEGTSYMFLTGPAVVKSVTGEVVTQEELGGAS
                     VHASKSGVAHFALPTEEEALSTIRELISYIPQNNMEEPPYLHTEDPIHRLDDVLNDII
                     PDSPNAPYDMYQVIYSIVDDNKFLEIHKNYARNIIVGFARMGGTSVGIVANQPKNLAG
                     VLDINSSKKAARFVRFCDAFNIPIVTLVDVPGFLPGTQQEYGGIILNGAKLLYAYGEA
                     TVPKVTITLRKSYGGAHIVMSCKQLRGDINYAWPTASIAVMGADGAVEVLYAKEIKAE
                     EDPDKRAAFIEAKKAEYEDLFSNPYNAAKYGYIDDIIEPRNTRFRIIRALEQLSTKKL
                     TNPAKKHDNLPL"
     gene            3927..4844
                     /locus_tag="PLI69_03565"
     CDS             3927..4844
                     /locus_tag="PLI69_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOG41600.1"
                     /translation="MKRILKIFLVVAGLGIALSANGQNQGSMRINEYLVINTDDFQDD
                     FGQQNSWIELFNSSYGTVDIGGCYLTDDPNNLKKYPIPGGDVLTAIKPRQHILFWADN
                     QPYRGTFHISFDLINAEEIILVKSDGVTIIDRIPVRKDLGPNVSFGRANDGVGSITGD
                     GEGWQIMERTTPSTNNITIDKTLKSNRMREMDPYGWMMAVLAMTVVFIALIILYLIFK
                     YIGKYNIQQGKKRVAAKSGKDISEVQYGELPGEAYAAIALALHLYKIENETHDEESYV
                     MTLQHTDRSYSPWSSKIYGLRQTPQLKRK"
     gene            4865..5299
                     /locus_tag="PLI69_03570"
     CDS             4865..5299
                     /locus_tag="PLI69_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOG41601.1"
                     /translation="MKEYKLKINGNNYLVVIGDVEDTSAMVEVNGTNYTVEFEKPVAK
                     SSAPIKIIRTSTPAAVSSVKPVQPIAPSAVSTPVKSPLPGVILEVKCAVGDTVKKGDV
                     ILILEAMKMENVIEATANGKIETIKVGKGDSVLEGDTLVVIG"
     gene            5307..>5590
                     /locus_tag="PLI69_03575"
     CDS             5307..>5590
                     /locus_tag="PLI69_03575"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOG41602.1"
                     /translation="MENILLNLKTFWQFTGFANLQWGHLIMIVIGCFFMYLAIKKEWE
                     PMLLVPIGFGILIGNIPLFAGLSVGVYEEGSVLNILYQGVQRGFYPPLIF"
BASE COUNT         1717 a         1184 c         1257 g         1432 t
ORIGIN      
        1 caatctgtgc ttcttccgca tcaaaattgc aaacatacct gtcggaagag gagaaaaaac
       61 tcttggtcat cgaaagataa tatacggcat caagcagatt ggtcttgcct gctccgttgt
      121 tgcccgatat gtagttgatt ttgggcgaaa attccagcga agcggcgact atatttttaa
      181 aattgtgaag tatgatgctt ttgaggtaca tattagtctt gtcagagttt acaaaagtac
      241 aaattattgt ttgtgtttcc catttttgtt gtaaatttgc attttataaa tacccgtgaa
      301 ggcgaatata aattacacga ataatacgat tataaatata gtacaataga tataacaatg
      361 gctaccaaga aaaaacattc cactcaggag gaagtaaaat taggaacagc gatttcttca
      421 attgaactct tctttcaaga caaaaaaaac cagcaaaggc tctttattgt cacttcagca
      481 attattgtaa tagtatttgg cataattgcc atcaacagat ggtatctcca gcctctaaag
      541 gctgaagcga gggcacagat gtttcctgca gagcagctct ttatggatgg cgactacgct
      601 acagccctca acggtgacgg caatgtgatg ggatttgctc aaattgctaa ccaatatggc
      661 aataaagccg gcaaaatcat ctatttctac aaaggtatat gccaacttca aaccggtgac
      721 aatgaaggtg caatcaagtc tctcaagaaa tatgacggaa aggacaagat tgccaaagca
      781 agagcttact gttgcattgg tgacgcctat gtcaatcttg acaaaaatac cgaagccctg
      841 aaatggtata agaaggctat tgcagaggag tctcacgcct acactgccgg ttatctgctc
      901 aaagccggca tcatcagcga agagatggga gacaatgagc aggcactcaa attctacgac
      961 gaaatttcca taaaatatcc caactctatc gaagcttacg aagttggcaa gtatatctca
     1021 cgcctgaaaa acaaatagtg ctatgggaag agcatttgaa ttcaggaaag agagaaaatt
     1081 caagcgctgg ggcaatatgg cccgtgtgtt tacccgcctc ggcaaggaaa tcaccatagc
     1141 agccaagcag ggtggtacag atccggcagg caatccacac ctcagaacgt tgatccagac
     1201 cgccaagtcc gagaatatgc ctaaagataa catcgagagg gctatcaata gggctatcga
     1261 gaaggatcag agtgattaca aagaggtagt atatgaaggt tacggccctc atggagtagc
     1321 tttcctcgtg gagactgcta cggataatac caaccggacg gtagcaaata tccgaagtta
     1381 ttttgcgaag tatggcggca gtctcggtac atcgggcagc gtagctttta tgtttgatca
     1441 taagtgcgct ttcaagatta agagcgaagc gcccgttgat ctcgaagaac tggaattgga
     1501 actgatcgat tatggtgccg acgaggtatt caaaaacgac gatgacgata taatcaacat
     1561 ctatggtgag tttactgcat tcaacaacat tcagaaattt attgaagaga aggggtttga
     1621 aatcgtatcc ggcggatttg agagatttcc aaatggagaa ctgaagaaag tgagtgctga
     1681 ggagagggta gagttgaata aacttatcga gaagttggaa gaggatgagg atgtacaaaa
     1741 tgtatatcac actctggaaa tagaggagta gagccgtttt ttctgaaaat taaaagtcgt
     1801 tcccttgggg aacgattttt tttattaaaa taaatggttt atatcacaat aattgttaag
     1861 tttgccgaat tgttaaaaat aattatctca taaaattatc attaataaat atggacttaa
     1921 ctcacattga acacatcggt atagccgtca aatctttgga agaggctatt ccctactatg
     1981 aagatgtact aggcctcaag tgttacaaaa ttgaagaggt agcagaccag aaggtcaaga
     2041 ctgcattctt ccgtattggt gacaccaaga ttgaacttct cgaaagcact tctcccgaag
     2101 gacccgtagc acaattcatc gaaaagagag gcgaaggcgt ccaccacatc gcatttgcaa
     2161 caagcagtgt aaaggattgc ctcgctaaag ccgaagccaa gggcctcaga ctcatagaca
     2221 agactcccag aaggggagcg gagggtctga acatcgcttt ccttcatccg aagtcaacaa
     2281 tgggtgttct gacggagttt tgcgaaaaag gcgaataatt aatcattaat attttaaaag
     2341 ttacaagaat gagtcatcaa ctcgaaaaag taaaagagct tatcgaactc agggaaaaag
     2401 ctcgtatggg tggtggtcaa aaggccattg atgcccagca tgcaaaaggc aaatataccg
     2461 ctcgtgagag gatcagtctg ctcctcgatg agggtagttt tgaagaattt gacatgttta
     2521 tgacccaccg ttgtaccaac ttcggaatgg aaaagaagac attccttggt gacggtgtcg
     2581 ttacaggtta cggtactatc gacggcagac ttgtttatgt ctttgcacag gactttaccg
     2641 ttatcggcgg ttctctttct gagactcttg ctctcaggat ctgcaaggtt atggaccagg
     2701 cgatgaaagt gggtgccccc atcatcggtc tcaatgactc cggtggagcc cgtattcagg
     2761 agggtgtaaa tgcccttgcc ggctatgccg agattttcca gaggaatatt ctagcatccg
     2821 gtgtagtccc ccagatttca gctatcctag gcccttgtgc cggaggtgct gtttattctc
     2881 ctgcacttac cgacttcaac ctcatggttg aaggtaccag ctacatgttc cttaccggtc
     2941 ctgcagttgt aaaatcagtt accggtgaag ttgttacaca ggaagaactt ggtggtgcca
     3001 gcgtacatgc ttcaaagagt ggtgtagctc actttgcact ccccactgag gaagaggctc
     3061 tctcgacaat ccgagaactt atcagctata tcccccagaa caatatggaa gagcctcctt
     3121 atcttcacac tgaggaccct atacaccgtc tggatgatgt actcaacgac ataatcccag
     3181 atagtcccaa cgctccttac gatatgtatc aggtaatcta cagcatagtt gacgacaata
     3241 aattccttga aatccataag aactatgcca ggaacatcat cgtcggcttt gcacgaatgg
     3301 gcggcacatc ggttggtatt gtggccaacc agcccaaaaa tctggccggg gtgctcgaca
     3361 tcaattcttc aaagaaagct gcccgtttcg tacgcttctg cgatgctttc aacatcccta
     3421 tcgtaactct cgtggatgtt cccggcttcc ttcccggcac acagcaggag tatggcggca
     3481 ttattctcaa tggagcaaaa cttctctatg cttatggtga ggctaccgtt cctaaagtga
     3541 ccattactct tagaaaatct tatggtggag cacacatagt tatgagctgc aaacagctac
     3601 gtggtgatat caactatgca tggcctacag ccagcatagc agtaatgggt gcagacggtg
     3661 cagtagaagt actctatgca aaagaaatca aagcagagga agatccggac aaacgtgctg
     3721 catttataga ggcaaagaaa gcagagtatg aagatctctt cagcaacccc tacaatgctg
     3781 ccaagtatgg ctacattgac gatattatag agcctagaaa tacccgcttc cgtattatcc
     3841 gtgctctgga gcagctctct accaagaagc tcacgaaccc tgcaaagaaa cacgataacc
     3901 ttccactcta attcaggaga atgccaatga aacgaatatt aaaaatattt cttgtagtcg
     3961 ccggattggg tatcgcactg agcgcgaatg gtcagaacca gggatcaatg cgaattaacg
     4021 agtatctcgt aatcaatacc gacgactttc aggatgactt cggacagcag aactcatgga
     4081 tagagctctt caactcgtcc tacggtactg tggatatcgg aggttgctac ctcacggatg
     4141 atccgaacaa cctgaaaaag tatcctatcc cgggtggtga cgtgcttaca gctataaaac
     4201 cgcgtcagca tatacttttc tgggctgata atcagcctta ccgcggaaca tttcacatct
     4261 ctttcgatct cataaatgct gaagaaatta tccttgtaaa gagtgatggt gtcaccatca
     4321 tagacaggat tcccgtacgt aaggatcttg gtcccaatgt ctcattcggt cgtgctaatg
     4381 atggtgtagg atcaataacc ggagatggag aaggatggca gataatggaa agaactaccc
     4441 cgagcaccaa caacatcacc attgacaaaa ctctcaaatc caatagaatg agggaaatgg
     4501 acccttatgg atggatgatg gccgtattag ctatgacggt agtgtttatt gcactaatca
     4561 tactctacct cattttcaaa tacatcggca aatataacat ccaacagggc aaaaagaggg
     4621 tagcagccaa atcaggaaag gatatatccg aggtccagta tggagagttg ccaggtgagg
     4681 cctatgctgc catagctttg gctctccacc tctacaaaat cgaaaatgaa acacatgatg
     4741 aggaaagtta tgttatgact ctccagcata cagacaggtc atattcaccc tggagttcca
     4801 agatatacgg tcttcgtcag actccacaac tcaaaaggaa ataatattta taactttaat
     4861 cgaaatgaaa gaatacaaac ttaaaataaa cggtaacaac taccttgttg taattggtga
     4921 tgtggaggat acttctgcta tggttgaggt caatggtacc aactacactg ttgaattcga
     4981 aaagcccgta gcaaaatcat cagctcccat aaaaattatc agaacttcca ctccggcagc
     5041 cgtttcttca gttaaacccg ttcaacctat agcaccctcc gccgttagta ctcccgtcaa
     5101 gtcacctctc ccgggcgtta tcctggaggt gaaatgtgcc gttggagaca cagtaaaaaa
     5161 gggcgacgtg atcctcattc tcgaggctat gaagatggaa aatgtaatcg aagctactgc
     5221 taacggaaaa attgaaacca taaaagtcgg caagggcgat tcagtacttg aaggcgatac
     5281 acttgtagta ataggctagt cttagtatgg aaaatatctt attaaacctc aaaacgtttt
     5341 ggcaatttac aggattcgcc aacttacagt ggggacacct gataatgatc gtcatcggct
     5401 gtttttttat gtatctcgcg ataaagaaag aatgggagcc gatgctgcta gttcctatag
     5461 gattcggtat cctcatcggt aacattcctc tatttgccgg cttgagtgtc ggagtttatg
     5521 aagagggatc agtattgaac attctctacc agggagttca gagaggtttc tacccgcctc
     5581 tgattttcat
//
LOCUS       DAOSIE010000082         5468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_13383_length_5468_cov_3.715500, whole genome shotgun sequence.
ACCESSION   DAOSIE010000082 DAOSIE010000000
VERSION     DAOSIE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13383_length_5468_cov_3.715500"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PLI69_03580"
     CDS             complement(<1..1015)
                     /locus_tag="PLI69_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41603.1"
                     /translation="MKRRVAFLLIVALAAASCSTTRILQEDECRLVENKVVITNDDDY
                     SASSLQLYIKQKPNDYFIGRWNPFLYVYNWSSGRGKGWDRFVEKLGVAPVIYDSTSID
                     ASKQGMLSHLEYSGYYGSDISHSVLKKEKKARVKYDVTLGKRYMIDSISYNIPDPYMR
                     ELMAADSANYTVKVGDMLSEKDLEEESERLASLFRTNGYYGFTKNYFFFYADTVRQRD
                     KADLTVDLQNYTRNESEESARPHKQYKIGSVSIVPMGGLRVRNKFLWQINTIKPGELY
                     NEQVINTTYERFASIRLFSSVNVQLSEADSSLVDCRIMLTPSKLQGVRANLEGSFNSS
                     GLFG"
     gene            1074..1799
                     /locus_tag="PLI69_03585"
     CDS             1074..1799
                     /locus_tag="PLI69_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOG41604.1"
                     /translation="MISRKEIKLIRSLYQKKYRDEEELFVVEGEKSVEEALRSGFEVI
                     KVFRISEIGEEAMSRITLLSSPSPALALVRKPAPLNASQIDNLIALRPLSLGLDSIRD
                     PGNLGTIIRLAEWFGIGTIFASRDTVDCFNPKVVQASMGSIFRTQIIYCDLLQIASEF
                     RAAGMSLFGTFLDGKSIYEESLGSNGLIVMGSESNGISPALESLVTHRITIPSFSPAS
                     EKRPDSLNVALATAIICSEFRRR"
     gene            1903..3759
                     /locus_tag="PLI69_03590"
     CDS             1903..3759
                     /locus_tag="PLI69_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOG41605.1"
                     /translation="MTATLIILIVTAALLVWGKIRSDIVALCCLLALVLTKVLTPTEA
                     LAGFSNSVVIMIAALFIVGGALSQTGLAGAVSNKLLKLAGNSSYKLFLLVMLVTAFIG
                     LFLSNTGTVALLMPIVVSMAFKSNTSASRLLMPMAYASSIGGMMTLIGTPPTLIMHDT
                     LIGAGYEGLQFFTTLPVGMILLVLGILLLWPLSKMLDKKDKKDQGDGSTTVKSPEQLA
                     SDYCLIDNIYRVRVIKDSPIIGKKLSELDITKRYSLIISEIHIRTIAQLSRSIRAVLP
                     EAGTILTENDVLYIIGEYKNVVRFVEENMLAFLDGQTEEQIEPPKFGGKFKFDEIGMA
                     EVVILQNSRLHNRLVKDSQFRNNYNVNILSIQRKDNYIIQDIKDVKIQAGDMLLVQGT
                     WENIDRLSRFERDIVVIGQPATEASKITLDHKAPYAAIILMLMVLAMVFNWFQPVIAV
                     LIAAVLMILGGCFRTVKDAYRTINWESVILFAGMMPLATAMEKTGTSALVTNGIVDAV
                     GSYGPYAVLAGIMAASSLLTMFISNTATAILFAPIGLQAAISMNVSPYPFLIGVAVAA
                     SMCLASPFSTPPNAMVMSAGKYNFMDYIKVGLPLQLLFLVVMIIVLPIIFPF"
     gene            3785..4675
                     /locus_tag="PLI69_03595"
     CDS             3785..4675
                     /locus_tag="PLI69_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41606.1"
                     /translation="MIIKEEINISDDYLSSGLVPVVRIMNKIYNCEDEKVIIDCSKIN
                     FVSPVFVISFMLFVFSCKKKVSLRNITTYLETIHFFNAIEADKIDPDKFISTLRTYLN
                     KTYIPVISFPALANRVADKDAILSAIENLLYKRLVIKTNVLIGLKYILGEMVDNITEH
                     SESVRGYIFAQFYPSKGYIDICIADQGITLKGSFDKVGMKYSGDVEAMSAANSQVSSK
                     NFPGSDNRGYGIYTSKNMLIDGLSGQFMMMSGTALYLKDNHIEDDLIELPKGFRLNGT
                     LIAIRLPYQNKDFLYSNYVE"
     gene            4709..5053
                     /locus_tag="PLI69_03600"
     CDS             4709..5053
                     /locus_tag="PLI69_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41607.1"
                     /translation="MERIFISQILSDDLKSRSSVHTLYVALKSKYINELVFDFSNVNF
                     ASRSFIDEFYNVFIKEHRPKLENVPDKIAVILDAVSRTQEENKKTITKKSKIKSFNSV
                     DEFCSYMTSIAF"
BASE COUNT         1550 a         1150 c         1159 g         1609 t
ORIGIN      
        1 ctccgaagag tcccgaggag ttgaaggatc cctcgagatt ggcccttacc ccttgaagtt
       61 tggagggagt gagcattata cggcagtcca ccaaagagga gtcagcttca ctgagctgta
      121 cattgacact actgaagagt ctgatacttg caaaacgttc gtaggtggtg tttatcacct
      181 gttcgttgta gagttcacca ggttttatgg tattgatctg ccagaggaat ttgttgcgta
      241 ccctgaggcc ccccatcggg acgatggaga ccgaacctat cttgtattgt ttatgaggac
      301 gtgcactctc ctccgattcg ttacgggtat aattttggag atcaaccgtc aggtcggctt
      361 tatcccgctg ccttaccgta tctgcgtaaa agaagaaata gtttttggta aaaccatagt
      421 aaccgttagt gcggaataga gaggcaagac gttccgattc ctcttcaaga tccttctcgg
      481 aaagcatgtc gcctaccttt acagtataat tagcggaatc cgcagccatc aattccctca
      541 tatacgggtc aggaatattg taactgatgc tatcgatcat gtagcgtttt ccaagggtaa
      601 catcatattt aaccctcgct ttcttctctt ttttaagcac tgagtgcgaa atatccgaac
      661 cataatagcc cgaatattcg agatgtgaga gcataccctg tttactcgca tcaatagagg
      721 tagagtcgta gatcaccgga gccactccca gtttttccac aaacctgtcc cagcctttac
      781 ctcttccgga actccagttg tagacatata ggaagggatt ccagcgaccg ataaagtagt
      841 cgtttggctt ctgtttgata tagagttgca gcgaagaggc ggagtagtca tcatcatttg
      901 taataactac tttattttcc acgagcctac attcatcctc ctgcagaatc cttgtggtac
      961 tgcaagaagc ggcagccagt gccacaatca atagaaatgc aactctcctc ttcattaccc
     1021 tgcaaattta gataaaatta ttattataat caggttaatt gttacctttg gatatgattt
     1081 ctaggaagga aataaaactt atccgctcac tgtaccaaaa gaaataccgc gacgaagagg
     1141 aactttttgt cgtagagggc gaaaaatccg tggaagaagc actccgatcg ggttttgagg
     1201 ttatcaaagt attccgaatt tccgaaatcg gagaagaggc aatgtcccgc atcacgctgc
     1261 tgagctcccc ctctcccgcc ctggcactag tgcgcaaacc cgcacccctc aatgcgtcgc
     1321 aaattgacaa tctgattgct ctgcgcccac tctcgcttgg actcgattcc attcgcgacc
     1381 ccggcaactt gggaactata atccgcctcg ccgaatggtt tggcatcggg accatattcg
     1441 catcacggga taccgtagat tgcttcaatc caaaggtggt gcaggccagc atgggttcaa
     1501 ttttccgtac gcaaatcatc tattgcgacc ttttgcagat cgcctcagag ttccgtgctg
     1561 ccggtatgtc tctattcggc accttcctgg acggtaaatc catctatgaa gagagtcttg
     1621 gctccaacgg cctcatagtg atgggctccg aatctaatgg tatctctccg gctcttgaga
     1681 gcctcgtgac gcatcgtatc acaattccga gcttttctcc ggcttcggaa aaaaggccag
     1741 attccctaaa tgtcgcccta gctaccgcta ttatctgttc cgaattcagg agaaggtaga
     1801 gagtgcttcg ttttttattt gaaaaatcga ataaaatgtc gtttttttgt aagcaaaata
     1861 gcctttaaaa gccttttata cctctgatac tccacaattg ttatgactgc aactttaatt
     1921 attcttattg taacagccgc actgctcgtt tggggtaaaa ttcgctccga tattgtagca
     1981 ctttgttgtt tgttggcatt ggtgttgaca aaggttctga cacctactga agctttggcg
     2041 ggcttttcca actccgtcgt gatcatgatt gccgcgctct ttatcgtggg aggagcgctg
     2101 tcgcaaaccg gacttgccgg cgcagtaagc aataaattgt tgaaacttgc aggcaacagc
     2161 agttataaat tatttttgct ggttatgctc gtgaccgctt tcatcggcct ttttttgagc
     2221 aataccggta cggtagcgtt gttgatgccg attgtggtaa gtatggcctt caaatccaat
     2281 acaagtgcaa gcagactatt gatgccaatg gcttatgcta gcagcatcgg aggtatgatg
     2341 actctgatag gtacaccacc aacccttatc atgcacgata cgttgattgg agcaggatat
     2401 gagggattgc agttctttac cactttaccc gtaggaatga ttctgttggt tttgggaata
     2461 ttattgcttt ggcccctgtc caaaatgctg gataaaaagg ataagaaaga tcagggtgat
     2521 ggaagtacaa ccgtcaaatc accggaacaa ctcgcttccg attactgtct tatagataat
     2581 atatatagag tcagagttat aaaggattcc cctatcatag gcaagaagct ttccgaactc
     2641 gatattacca aacgatactc attgatcatt agcgagatcc atattcgaac aattgctcag
     2701 ttgagccgtt ccatacgtgc cgtactcccc gaagcgggta ccattctgac agaaaatgac
     2761 gttctctata tcattggcga gtataaaaac gttgtgagat ttgtagaaga aaacatgctc
     2821 gctttcctcg atggtcaaac ggaagaacaa atagaacctc ccaaatttgg cggaaagttc
     2881 aaattcgacg agattggaat ggcggaagta gttatccttc aaaacagccg cctgcacaac
     2941 cggttggtaa aagactctca atttcgcaac aattacaatg taaatatcct aagcatacag
     3001 cgtaaagaca attatatcat acaggatata aaagatgtaa aaatacaagc cggcgatatg
     3061 ttgttggtac agggtacctg ggagaatatc gaccgtctga gccgttttga gagagatatt
     3121 gtcgttatcg gacagccggc gaccgaggct tctaaaatca ctctcgatca caaagcccct
     3181 tatgcagcca tcattttgat gttgatggtg ctggcaatgg tattcaactg gtttcagcct
     3241 gtaatcgctg tgttgatagc agctgtcctg atgattctcg gcggctgctt cagaacggtt
     3301 aaagatgcat atcggacgat aaattgggag agtgttatcc tttttgcggg aatgatgccc
     3361 ctggcaaccg ccatggagaa aaccggtact tcggccttgg taaccaacgg tatagttgat
     3421 gcagtgggtt cttacggacc ctatgccgtt cttgccggga ttatggcagc ctcctctctg
     3481 ctgactatgt tcataagcaa tacggctacc gccattctat ttgccccgat aggcttacag
     3541 gcggccatat caatgaatgt aagtccctat cctttcctaa tcggtgtggc ggttgctgca
     3601 agtatgtgtc ttgccagccc cttctccacc ccgcccaacg caatggttat gtcggccgga
     3661 aaatacaatt tcatggacta catcaaagta ggcctcccct tgcagttgct tttcctggtg
     3721 gtaatgatta tagtgctgcc gataattttt ccgttttagg gtagaaataa tctatcttcg
     3781 caccatgatt ataaaagaag aaataaatat ttctgatgat tatctttcaa gtgggcttgt
     3841 tccggttgta agaataatga ataagattta taactgtgag gatgaaaagg tgattattga
     3901 ttgttctaaa atcaactttg tatctcccgt attcgttatt tcctttatgc tttttgtatt
     3961 ttcctgcaag aaaaaagtat ctctcagaaa tattacgacg tacttagaaa caatacattt
     4021 ttttaatgcg atagaggccg ataagattga cccagataag tttatttcaa cactgcggac
     4081 atatctaaat aagacataca tccctgtgat aagttttccc gccttagcta accgagtggc
     4141 agataaagat gctattctaa gtgcaataga gaatttgtta tataagcgat tagttattaa
     4201 aacaaatgtt ttgataggac ttaagtatat attgggagaa atggttgaca acattactga
     4261 acattcggaa tcagtaagag gatatatatt cgcacaattt tatccttcaa agggctatat
     4321 tgatatttgc attgcagacc agggtatcac cttgaaaggc agttttgata aagtcggcat
     4381 gaaatattcc ggagatgttg aagctatgag tgctgcaaac agtcaagtat catcaaaaaa
     4441 cttccctggt tctgataatc gtggttatgg aatatatacc tcaaagaata tgttgattga
     4501 tggtctttcc ggacaattta tgatgatgtc tggaactgca ttgtatctaa aagacaatca
     4561 tatagaagat gacttaatag agttacctaa aggtttccga ttaaatggta cactaattgc
     4621 aataagatta ccatatcaga acaaagattt tttgtattct aactacgttg aataaaaaaa
     4681 tgctatattt gcactaagca actattctat ggaaagaatt ttcatatcac agatattgtc
     4741 agatgacctt aaatcaaggt cttctgtgca tactttatat gttgctttga agagcaaata
     4801 tattaacgaa ttggtttttg acttttcaaa tgtcaatttt gcatccagga gttttattga
     4861 tgaattctac aatgttttca taaaagaaca ccgtccgaaa cttgaaaacg ttcctgacaa
     4921 aattgctgtc attcttgatg ctgtatcgcg aactcaggaa gaaaataaaa agactattac
     4981 taagaaaagc aaaatcaagt ctttcaattc agtggatgag ttttgctcat atatgacttc
     5041 aatagctttt taatagctaa cactaaaata gatgtagaca aaagtgttgc cgttaacaat
     5101 atagcaacac ttttttttgt taagggtgct gcaatgccgg attgatttgt tgcactgtct
     5161 gctagatgtc tgaacatgcg attaaacggc aaatgctaaa gcagtatgta gaaagcaaaa
     5221 attggaaaca actctcgtta tttccaattt tgttactcgg aactcggtac tcagaactca
     5281 gaacttgcga ctacaatcca aggactctcc tgaaccacga tttctttttg gtctgggtgt
     5341 agctataacc atagccggca tagccgtaac catagccgta gccgtaacca tatttgcggc
     5401 catagccata accgtagcca taactgccgt aagaggatat tttcttgaca tcgattccgt
     5461 ttagcagg
//
LOCUS       DAOSIE010000083         5448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_13439_length_5448_cov_4.246616, whole genome shotgun sequence.
ACCESSION   DAOSIE010000083 DAOSIE010000000
VERSION     DAOSIE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13439_length_5448_cov_4.246616"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1886
                     /locus_tag="PLI69_03605"
     CDS             <1..1886
                     /locus_tag="PLI69_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG41608.1"
                     /translation="GNLNRYDAGAVKVRVFEVDKEDKKAWMQEALDALAGKEDDIKDL
                     GEIARLTYDGYELRDITILVRTNNEGKMVVDKLISKGINIVTEEVLVLGYSRAVLKIV
                     TLLKYRDNPDDQVNQLMLKTLFTENQESNISDLTGKELAEMPLKEGCEAIFTALFNED
                     QRDNLKALSLYESCEVMLRSIYDEIPEYEIEFVQAFLDEVNRFMTRNGSDLPAFLEWW
                     EESGQNVKINSSSDQNAVRVMTIHKAKGLGFKAVIIPFFNEKTSVKTFPIPPYLWCIP
                     DIDELKNIGLIPVKYTTKENTGFEKDFETERKETYIDTLNIAYVAMTRAYFEMVIYTP
                     DETEKKKEGQDQKQPKDSMAQKLIKFLKEESQNGDNGIYIVEAREKEESLEEGEAREK
                     KESQKEESGRYIKIKDLGDGCYEARANNGQWRYSDAKQIAKEQQPGATKKTEVLEKGI
                     ETTTVKSFRSIPIGERLEQSLRSRDFFEFEKDPLESPRIKGIVLHDILSEICTIDDLQ
                     AAVERAVIQGDLKKSESGDAKDFLYRRLQSVAERHWFDGTYDTLNEQEILVKNGDTLR
                     PDRILTGDGRTMVIDYKFGERDNKYLKQVRGYMEKLTQMGLTEVSGYIWYLKDNEVEQ
                     VLL"
     gene            1932..2729
                     /locus_tag="PLI69_03610"
     CDS             1932..2729
                     /locus_tag="PLI69_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOG41609.1"
                     /translation="MELTSYHVDAFTDQLFKGNPACVVQLGEWLADDLMLKIAAENAV
                     AETAFFKKNKDSGGYSIRWFTPDLEMDLCGHATLATSFVIDMISKEPLNEILFNSASG
                     ELRVVVNNGLFTLNFPSRPAVPSTIPRETYDSLSIKPLEVFKARDYLLIYESQQQIEE
                     IAIDRNLFDMVNMDPGGVIITAPASEPEVDFVSRFFTPQSTILEDPVTGSAHCTLIPY
                     WSARLGKRVMTAKQISPRGGTLYCEDCGDRVLISGTARLYSRSTLYL"
     gene            complement(2734..3252)
                     /locus_tag="PLI69_03615"
     CDS             complement(2734..3252)
                     /locus_tag="PLI69_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG41610.1"
                     /translation="MKPQITIRQATKEDIALIRDLAQIIFPHTYRKMLSQEQIDYMME
                     WMYSPENIAAGIDSGQEYFIVEVEGEAVSYAGIAPQEDDPDGTKVFELMRLYILPEYQ
                     KTGLGKLLFEHVCDHIRSKGFKKSRMMLHVNRENPAVQFYLHQGMRILYSKDFDIGNN
                     FWMNDHIMGMDL"
     gene            complement(3351..4013)
                     /locus_tag="PLI69_03620"
     CDS             complement(3351..4013)
                     /locus_tag="PLI69_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOG41611.1"
                     /translation="MKKLILMAVVLLMAVGAAAQERYQKVIPPRRYEEANKEVKVRPL
                     AVLFGDSITDAWPIMSPEFFTINNLVGRGISGETTSDMLIRFQKDVVALRPKYVVILA
                     GTNDIAMNDGPISLEHILDNIISMCQIARANNIQPILCSVLPAYLYRWRTELTPAEEI
                     VELNEMIRAYAKSTRIPYVDYHSQMKDERNGLPENLGPDGVHPNLEGYHMMESILIDY
                     LK"
     gene            4936..5181
                     /locus_tag="PLI69_03625"
     CDS             4936..5181
                     /locus_tag="PLI69_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41612.1"
                     /translation="MTQQTRTVITVILIVILVVSLVIRFLKLWYADRFTESEIEEEYE
                     TSSAESETKFTKIYKRIMELAKICEQISIRQEELMSI"
BASE COUNT         1746 a         1127 c         1132 g         1443 t
ORIGIN      
        1 aaggcaacct caaccgctat gatgcaggag ctgttaaagt cagagttttt gaagtcgata
       61 aagaggataa aaaagcctgg atgcaggagg ctctggatgc tcttgcaggc aaagaagatg
      121 acataaagga tcttggcgag atagcacggc ttacatacga tggatatgag ctacgcgaca
      181 tcacaattct tgtccgtacc aacaatgaag gcaaaatggt ggtggataaa ctgatttcaa
      241 aggggatcaa catcgtcact gaagaagtcc tggtgcttgg atattccagg gccgtcctca
      301 agattgtgac acttcttaaa taccgggata atccggatga ccaggtaaac cagttgatgc
      361 tcaaaactct ttttacggag aatcaagaga gcaatataag cgatttaacc ggtaaggaat
      421 tagccgaaat gccacttaaa gagggttgtg aggctatatt tactgcccta tttaacgagg
      481 atcagagaga taatctgaaa gctctctcac tttatgaaag ttgtgaggtt atgctcagaa
      541 gtatatatga tgagataccg gagtatgaga ttgagtttgt acaggcattt ctggatgagg
      601 ttaatagatt tatgacgcgt aatggatccg atttgcctgc atttctggag tggtgggagg
      661 aaagtggtca gaatgtaaaa atcaattcct cttccgacca aaatgctgtg cgtgtaatga
      721 caatacacaa agcaaaagga ctcggcttca aagcggtcat tatcccattt ttcaacgaaa
      781 aaacctcagt aaaaaccttc cctatccctc cctatctctg gtgcataccg gatattgatg
      841 agctaaaaaa cataggcctg attcctgtta aatacactac caaggagaac actggctttg
      901 aaaaggattt tgaaacggaa agaaaagaga cctatattga cactttgaac atcgcttatg
      961 ttgcgatgac gcgcgcctat tttgaaatgg tgatctatac tcctgatgaa actgagaaaa
     1021 agaaagaggg tcaagaccaa aaacaaccaa aagattctat ggcccaaaaa ctcataaagt
     1081 ttctcaagga agaatcccaa aatggggaca atggtatcta tatagtagag gctcgggaaa
     1141 aagaagagtc tctggaggaa ggtgaagctc gggagaaaaa agaatctcag aaggaagaaa
     1201 gcggtaggta tataaaaatt aaagatttag gcgatggatg ctacgaagca cgcgcaaata
     1261 atggccaatg gcgttatagt gacgccaagc aaatagcaaa ggaacagcaa ccgggagcaa
     1321 ccaaaaagac cgaagttctc gaaaagggca tcgaaacaac aactgtcaaa tcattccgct
     1381 caatacctat cggagagaga cttgagcaat cactgcggtc gcgcgacttt tttgaatttg
     1441 aaaaggatcc tttggagagt ccccgtatca aaggaattgt actccacgat atcctatcgg
     1501 aaatttgcac cattgatgat ctgcaagcag ctgttgagcg ggcggttatt cagggagatc
     1561 taaaaaaatc agaatccggg gatgcaaagg attttctcta ccggagacta cagtcagtag
     1621 cggagagaca ctggtttgat ggtacttatg atacacttaa cgaacaagag atacttgtta
     1681 aaaatgggga tacacttcgt ccggacagga ttctcacggg agatgggcgt actatggtga
     1741 tagattacaa gtttggagaa cgcgataata aatatcttaa gcaggttcgc ggttatatgg
     1801 agaaactgac ccaaatgggc ttaactgaag tatcgggata tatctggtat ctcaaagata
     1861 atgaagtaga gcaagtatta ttataaaagc ctattattta ctatttttag aaacactgaa
     1921 atttgtccta tatggaactc acttcttacc atgtcgatgc ttttacagac caactcttca
     1981 aaggcaatcc agcttgtgtg gttcagcttg gggagtggct tgcagatgat ttgatgctta
     2041 aaatcgcagc tgaaaatgca gtggctgaaa ccgcattttt caagaaaaac aaagactccg
     2101 gaggctattc catacgctgg tttactcctg atctggagat ggatctttgc ggtcacgcca
     2161 ctttggcaac ctcttttgtc atcgatatga tctcgaaaga accactcaat gagatactct
     2221 ttaactccgc tagcggagaa ttgagggtag tagtaaataa cggactcttt acacttaatt
     2281 tcccttcgcg accggcagtg ccgtcaacaa ttcccagaga gacttatgat tctctgagta
     2341 ttaaaccatt ggaggtcttt aaagcccgcg actaccttct gatatacgag tcacagcaac
     2401 agatcgaaga gatagcgata gaccgcaacc tatttgatat ggtaaatatg gatccgggag
     2461 gagtgatcat aactgccccc gcttcggaac ccgaagttga cttcgtatcc cgcttcttca
     2521 ccccacaaag caccattttg gaggaccctg tgaccggttc ggctcactgc acgcttatcc
     2581 cctactggag tgcacgtctc ggcaaacgcg ttatgaccgc caaacaaatt tcgccccgcg
     2641 gaggtaccct atattgcgaa gattgcggcg accgcgtcct catttccggc accgcccgac
     2701 tctactcccg cagcacgctc tatttgtgag aattcagaga tccatgccca taatatggtc
     2761 attcatccag aagttattgc caatgtcaaa gtccttgctg taaaggattc gcatcccttg
     2821 atgaaggtaa aactgtactg ccggattctc gcgattgaca tgtaacatca ttctgctctt
     2881 tttgaagccc ttgcttcgaa tatggtcaca aacatgctca aacaatagtt taccaagtcc
     2941 ggttttctga tattcaggta gaatatatag gcgcatcaat tcaaatacct tggtgccgtc
     3001 cggatcatcc tcttgaggcg caatacccgc atagctcacc gcctcccctt ccacttccac
     3061 aataaaatat tcctgccccg aatcaattcc tgctgcaata ttttccggag agtacatcca
     3121 ctccatcata tagtcaatct gctcctgtga gagcattttt ctgtaggtat ggggaaaaat
     3181 aatttgcgcc aaatcgcgaa tcagagcgat atcctctttg gtcgcctgac ggatggtgat
     3241 ttgcggtttc atactttaat aagatagtct gcgatctatt atgcaatact tgtctgtgct
     3301 ggtaagctct cctatactgt caggtgctat gctgtaatat cgattaagtg ctacttgagg
     3361 taatcaatca aaatcgattc catcatatgg tagccttcaa gattggggtg tacgccgtcc
     3421 ggcccaagat tttccggaag tccgttgcgc tcatccttca tctgcgagtg ataatccaca
     3481 taaggaatac gggtactttt tgcgtaggca cggatcatct cattaagctc tactatttcc
     3541 tccgcagggg taagttccgt acgccacctg tatagataag ccggcagcac ggaacagagt
     3601 atgggttgga tgttatttgc cctggcaatc tggcacatcg atattatatt gtcgaggata
     3661 tgctccagag agatcggccc gtcgttcatt gcaatatcgt tggttcccgc caggattacc
     3721 acatatttcg gcctcagagc cacaacatcc ttctggaacc taatcagcat gtcactcgta
     3781 gtctctccgc tgataccgcg accgactaga ttgttaatgg taaaaaattc cgggctcatt
     3841 ataggccagg catcggtaat ggaatccccg aataatacag ctaaaggtct cactttcact
     3901 tctttattag cctcctcata acgcctggga gggattactt tctggtatct ttcctgcgca
     3961 gcagcaccca cagccatcag cagtaccacc gccattaaaa ttaatttttt catactgcaa
     4021 aaataggaaa aaggaaggag aaaatgaggc attacagcca agaaatggga tttgcaagaa
     4081 agggtattta caaggaaggg cataaaatac aacaggaaga cttcaataat gaaatctccc
     4141 tgaggccttg gtccaaacct ttcggatagg tccaatgcaa atataatgat tattttacaa
     4201 agaaaaatat ttctataatg aattgtctct tttattaaaa gtaagacccc aaggcagtaa
     4261 tctgaaacta caatccagca cttgcctcgg agtctatgct gtaaaattaa tgaatttcta
     4321 aatacttaac aaatatcgtc tttgtttttc aattggcata agccgaataa acataaaaat
     4381 aaaagagaac tcgtattaac gaattccctt ccagtgaatc agtgcaatcg gcgtacccat
     4441 cgctcatcac attgcaaata tagaacattt tcaaataata agcaatttat tcgcactaaa
     4501 tgtgcaatta ttgcaataac tatccaaaaa aggcaccggc aactacggaa aaatgaaaga
     4561 agtttagaag ataggttaca ctccatgcgg atgatcttta caccatttta tgaaactcac
     4621 cacccgacga atggccaaat aagcaacgat tattacaaag gtcgtcagaa acggccctgc
     4681 tgcaacaatg aggccaacta gactggatag aaactcacgc ataacagtac aaatatatat
     4741 ttatttctcc aaaagagcaa tattgattta ttaactgatt agatctttgt atctttgatc
     4801 tatcgcaaac ggccatgaag tgaataaaaa ttttacaaga gtaaaacacg atgttaaaac
     4861 tcttctctgt aaagaatatg aacgaatagc ccctatggct tcttacgaga atatggatga
     4921 ttaaacatta aaaaaatgac acaacaaacc agaactgtaa ttactgtaat actcatagtt
     4981 atacttgttg tttcgcttgt gataagattt ctgaaactat ggtacgctga ccggttcact
     5041 gaatcagaaa ttgaagaaga atatgaaaca tcgagtgcag agagcgaaac taagtttacg
     5101 aaaatatata agagaataat ggaacttgca aaaatttgcg aacaaatttc cattcgacag
     5161 gaagaactta tgagcatcta gaagaaaaat gagaagaaaa ctaaagaact ggaaaataaa
     5221 attaaaaaac ttgaaaataa attggataca aagtaatcat agcggttgtg aattgacttg
     5281 tattagcaaa gactttatct gacagccaag gctctttatt acgtattcaa taatgagttt
     5341 tcaaaatgct gctattggaa gccatattga tactaccttg caaacataat ctacctacct
     5401 tattttttta acctatcaat caacagtgct cttacaactt tatatccc
//
LOCUS       DAOSIE010000084         5417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_13533_length_5417_cov_3.867773, whole genome shotgun sequence.
ACCESSION   DAOSIE010000084 DAOSIE010000000
VERSION     DAOSIE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13533_length_5417_cov_3.867773"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PLI69_03630"
     CDS             complement(<1..841)
                     /locus_tag="PLI69_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41613.1"
                     /translation="MSKYESNLFWNPRDKIDEKNIERTIYAFESLLLQYNTRTRNVIA
                     NYRRNFKNDRAFFNEFLSLKRKEILSWRNCGVKTTRNVVEIIRQLRSRLPSESEQSLV
                     HDIPMLESDTILRLFSLKEKLGYFPVFATLKEYIATFDDRDLRVLNSLLLIHDGKLPI
                     NRIIVAEELGISIERVRQMMLNIIYRIEDYLGMMKKMIKTPRGYGKCPYRWRSTGVHN
                     DVNKAEETNFSRSIINMAFAILYREISIVGSVRDSLMMKNDRFVRTTLVPSKLAKYFD
                     FEGF"
     gene            972..2222
                     /locus_tag="PLI69_03635"
     CDS             972..2222
                     /locus_tag="PLI69_03635"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOG41614.1"
                     /translation="MINIETYDFTGKKAIVRVDFNVPLNDNFEITDDTRIKAAIPTLK
                     KVLEKGGSLIIMSHLGRPKGPAEKFSLKHIISRIEYYLGTKVQFAEDCQKADAQAAAL
                     KPGEVLLLENLRFYAEEEGKPRGLADDATDEEKKAAKAAVKESQKAFVAKLASYADCY
                     INDAFGTAHRAHASTALIAKYFPKDKMFGYVMEGEIEAIDKILNNPARPLTAILGGAK
                     VSSKIGIIENLIDRVDHLIIGGGMVYTFIKALGGKIGNSICEDDQIETAKNIMAKAKA
                     KGIDLDLRREVVVADNFSNDANIKIVNNFEIPDGWEAMDAAPSSLENWQKIIDDSGTI
                     LWNGPLGVFEMPNFAKSTNAMAEMLAKATEKGAFTLVGGGDSVAAVTQMGYADKVSYV
                     STGGGAMLEYLEGIELPGIKAIRE"
     gene            2232..3140
                     /locus_tag="PLI69_03640"
     CDS             2232..3140
                     /locus_tag="PLI69_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOG41615.1"
                     /translation="MTVEYPSSAWRDYELIDSGNFEKLERFGRYVMIRPEIKALWEPA
                     LPQDDWIKRAHTKFVIGDGLGKAGKEDSGSWNILRKMDKEWVISYPKAELDLRLKLTS
                     FKHIGIFPEQAPNWDYIYKSVKEIAASSPGGAPPRILNLFAYTGAASLAARRAGAEVT
                     HLDSVRQVVTWARANMETSGLDNIRWVIEDALLFVKREARRGNFYDGIILDPPAYGYG
                     PDGKRWKLDDSIYEMLTQLSSILAPERSFMLLNLYSNGYSPVMAETLVSCAFGGKFGS
                     LDCGESVLRDSFGKLLPMSVFARLKR"
     gene            3169..4040
                     /gene="lgt"
                     /locus_tag="PLI69_03645"
                     /pseudo
     CDS             3169..4040
                     /gene="lgt"
                     /locus_tag="PLI69_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133576.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
     gene            complement(4084..4539)
                     /gene="smpB"
                     /locus_tag="PLI69_03650"
     CDS             complement(4084..4539)
                     /gene="smpB"
                     /locus_tag="PLI69_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOG41616.1"
                     /translation="MAKRKENRIDIRNKKATFDYELLEHFTAGIVLSGSEIKSIRAGK
                     ASLVDSYCYFVENELFVKNMHIAEYWWGSAFSRQDPKRDRKLLLTKKELRKLRRATRE
                     KGMTIIATRLFISENGYAKLNISLARGKREYDKRHSIKEKDMRREMERI"
     gene            4650..>5417
                     /gene="miaA"
                     /locus_tag="PLI69_03655"
     CDS             4650..>5417
                     /gene="miaA"
                     /locus_tag="PLI69_03655"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOG41617.1"
                     /translation="MLKPPFEEYDLICVLGPTASGKTRYAVKLARELDGEIISADSRQ
                     IFRGMDIGTGKDLEEYGEIPYHLIDIADAGTKYNIFEYQKDFERAYQDILQRGKTPIL
                     CGGSGLYIEAATCGYYLPEVPPDMALRAELEKLPTEELIAKYESLRKPHNTTDYDTRK
                     RLIRALEIAIWEEKHPVKRSSFLPKKTKFIGISVSREVRNARIDARLEARLANGMVEE
                     VRQLLDSGIPPEDLIWYGLEYKFVTMYLTGQMEYEEMV"
BASE COUNT         1420 a         1168 c         1320 g         1509 t
ORIGIN      
        1 gaaacccttc aaagtcgaag tatttagcca gcttggaggg aacaagtgtc gttctgacaa
       61 aacggtcatt tttcatcata agagagtctc gcacgctccc tacaatggat atttcccgat
      121 agagtatggc aaaagccata ttgatgatgc tgcggctgaa attggtctcc tcagctttat
      181 tgacatcgtt gtgtactccg gtagagcgcc agcggtaagg acatttgcca tatccccgcg
      241 gggtcttgat catctttttc atcatcccga ggtaatcctc gatgcgataa ataatattca
      301 gcatcatttg gcgtacccgc tctatggaga taccgagttc ctctgccact atgatgcggt
      361 ttattggcaa tttcccatca tggatcaaca gtaatgagtt cagaactctt agatccctgt
      421 cgtcaaatgt ggctatatac tctttcagag tagcgaatac gggaaagtag cccaattttt
      481 ccttgaggct gaagagtctt aggatagtat cgctctccag catcgggatg tcatgtacca
      541 gactctgttc actctcactg ggcagcctgc tgcgcagttg tcggatgatt tctactacat
      601 tacgagtggt tttgactccg caattacgcc aggagaggat ctcttttctt tttagagaga
      661 ggaattcatt gaagaatgcc cggtcatttt tgaagttcct gcggtaattg gcgattacat
      721 ttcgtgtgcg ggtgttatac tgaagaagga gagactcgaa ggcgtagatt gtgcgttcga
      781 tatttttctc atcgatttta tcccggggat tccaaaatag atttgactcg tattttgaca
      841 tgacaaaaag tttttacaaa gtttaaggaa tcggggcata caaatataat taatatattg
      901 aaggttaaca actttttgtc aaatatttta taattttgca gccgacaaaa aagattttat
      961 acagttattt tatgattaat attgaaacat acgactttac cggcaaaaaa gccattgtaa
     1021 gagtcgattt taacgttccg ttgaacgata attttgaaat tacagacgat acccgcatta
     1081 aggcggctat tcctacattg aaaaaggtcc tggaaaaggg agggtcactt atcataatgt
     1141 cacatctcgg ccgtcccaag gggccggcgg agaaattctc tttgaaacac attatttccc
     1201 gtattgagta ctatctcggt accaaggtgc agtttgcaga agattgccaa aaagctgatg
     1261 cgcaagcagc ggcattaaag ccgggcgaag tgcttctact ggaaaacctt cgtttctatg
     1321 cagaagaaga gggtaaaccc cgcggactgg ctgatgatgc taccgatgag gagaagaagg
     1381 ctgctaaagc tgccgtaaag gagagtcaga aagcatttgt tgcgaagctt gcttcctatg
     1441 cagattgcta tatcaacgat gctttcggta cggcacaccg tgctcacgca tctaccgcgc
     1501 ttattgcaaa atatttccca aaggataaga tgttcggata tgtgatggag ggtgaaatag
     1561 aggcgattga caaaatacta aacaatcctg cacgccccct taccgcaatt ctcggcggag
     1621 caaaggtctc ctccaagatt ggtattatcg agaatcttat cgatcgggtg gaccatctca
     1681 ttatcggtgg gggtatggta tatactttca ttaaagccct cggtggaaaa atcggcaatt
     1741 ccatctgcga agatgatcag atagagacag caaaaaatat tatggctaaa gccaaggcta
     1801 agggcataga cctggatctc cgtcgggagg tagtggtggc tgacaacttc agcaatgatg
     1861 caaacataaa gattgtaaac aatttcgaga ttcccgatgg ctgggaggcc atggatgccg
     1921 caccctcttc cctggaaaat tggcagaaaa tcatagatga ctccggcact attctttgga
     1981 atgggcccct cggagttttc gagatgccca attttgccaa atccaccaat gctatggcag
     2041 aaatgcttgc caaagctacg gaaaaaggcg cttttactct cgttggcggt ggtgattcgg
     2101 tggcagccgt aactcagatg ggctatgccg acaaggttag ctatgtctcc accggaggcg
     2161 gtgcaatgct cgaatatctt gagggaatag aactccccgg catcaaggct atccgagagt
     2221 aatacttccg aatgactgtc gaatatccat cttcagcctg gcgcgactat gagcttatag
     2281 actccggaaa ctttgaaaaa ctggagcgct tcggcaggta tgtgatgatt cgacccgaaa
     2341 tcaaggccct ttgggagcct gcactgcctc aggatgactg gataaaaaga gcccatacaa
     2401 agtttgtaat cggtgatggt ctaggtaagg caggtaagga agatagcgga tcctggaata
     2461 ttcttcggaa aatggataag gagtgggtga tctcctatcc gaaggcggag cttgaccttc
     2521 gcttgaaact aacctccttc aagcacattg ggatattccc cgaacaggcc cccaattggg
     2581 attatatcta caaaagcgtt aaggagatcg cagcctcttc tcccggtggc gctcccccca
     2641 ggatacttaa tctattcgca tataccggag ctgcttcact ggctgctcgt cgggccggag
     2701 ccgaggtaac tcatctggat tcggtacgcc aggtggtaac ctgggcgcgc gccaatatgg
     2761 aaaccagcgg tttggacaat atacgctggg taattgaaga tgccttactt ttcgttaaac
     2821 gggaagcacg cagaggcaac ttctatgatg gcatcattct tgatcctccg gcatacggct
     2881 atggtcccga tggtaagaga tggaaacttg atgattctat ctacgagatg ctcacccaac
     2941 tctcttccat attggctccg gagaggagtt ttatgctgct caacctctac tccaacggct
     3001 actcgcccgt tatggcggag actctggtaa gttgtgcctt cggaggtaag tttggttcgc
     3061 ttgattgtgg agaatcagtg cttagggaca gcttcggcaa attgctccct atgagtgttt
     3121 ttgcaaggtt aaaacgttga ttttataatt atagataaat actaacagat tatggatttt
     3181 ttatcggtag tatggaatgt ggatccggtg atattcagtc tcggtccctt aaccgtcaga
     3241 tattacggac tgctcttcat agcaggtttc cctttaggat attggctttt cacaaaattt
     3301 tacaaaaaag agggggtcga tgtcaatttg cttgagccgc tactttacgc attgctcatc
     3361 ggcaccatcg ttggagctcg tttaggacac tgtctcttct atcagcccga ctactatttg
     3421 accaaatccc actggatcga gatattcatg ccatggaaag ggggtcttgc gagccacggc
     3481 ggtgccatag gagtactttt ggctatttgg tggtattctc gtaaatatgg ccctaagaat
     3541 ggttttgaca tgatgtggat tttggatagg attgcaattg ctgtcgcctt cgccggatgc
     3601 tttatacgtt tgggcaacct tatgaattct gagatttacg gcaatccgac agatctgccc
     3661 tgggatttat ttttgtacag agaggcgaga ctgtggccaa gcatccgaca caactatatg
     3721 aggctttgag ttatctgttg ttgggttttg cactgcttgc tacttaccgt taccgtctca
     3781 aggatcttaa aaagggtact ttgtttggaa ttttcctcat agtgcttttt ggaatgagat
     3841 tcctcatcga atatgtcaaa gagcctcagg tggctttcga agaggggatg gctctgaata
     3901 tgggccaact gctaagcata cctttcattg ttgcgggggt agtaatagtt gtgtggagtc
     3961 tggtggcgaa aaagcctgcg atggctatta ttaagccaaa gcaaaatcac cgagccgctc
     4021 ccaaggataa gaagaagtag tatcagacga tacttcgtaa ttaaccgatt ctttcagcta
     4081 ctgtcatata cgttccatct cgcgacgcat atccttctct tttatagagt ggcgtttatc
     4141 gtattcacgt ttaccgcggg ccagtgagat attgagtttg gcatagccgt tttccgatat
     4201 gaaaaggcgt gtggcaatga tggtcatacc cttttcgcgt gttgcacggc gcaatttgcg
     4261 caattctttc ttagtcagca gcagtttgcg gtcccgtttt gggtcctgac ggctgaatgc
     4321 ggagccccac cagtattcgg cgatatgcat attctttaca aagagttcat tttcgacaaa
     4381 ataacagtag ctgtccacca gcgaggcctt gcccgcgcgg atagacttga tctcgctgcc
     4441 acttaaaacg attcctgcgg tgaaatgttc gagcagttcg tagtcgaacg tggccttctt
     4501 gttccttatg tcgattctat tttcttttct ctttgccata cggatatgac gattcttttc
     4561 tcatttgctt gcatcaatag atgcacaaat ggtgcgaaac gttgcaaaat tagtttattt
     4621 ttgtggaata aaccaaagga gctcccgata tgctaaagcc cccatttgag gaatatgatc
     4681 tgatatgtgt cctgggcccg actgcctccg gtaagactcg ttatgcagta aaactggctc
     4741 gtgagttgga cggagagata atttccgccg attcccggca gatattcagg gggatggata
     4801 taggcacggg caaggatctg gaggagtacg gagagattcc ctaccatttg atagatatcg
     4861 ctgatgccgg caccaaatat aacatttttg aataccagaa ggattttgag cgggcatatc
     4921 aagatatttt gcaaaggggc aagactccga tattatgcgg cggctccgga ctctatatcg
     4981 aagctgcaac ctgcggctat tatctccccg aagttccccc tgatatggcc ctgagggctg
     5041 aattggagaa acttcccaca gaggaactca tcgccaaata tgaatcattg cgtaaacctc
     5101 ataacactac tgattatgat acacgcaagc gtcttatccg tgctttggag attgcaatat
     5161 gggaggagaa gcaccccgtc aaaaggagtt catttttgcc taaaaaaacc aaatttatcg
     5221 gcataagcgt ttcacgcgaa gtgcgcaatg cccgcattga tgcacgtctc gaagcacgcc
     5281 ttgccaatgg catggtggaa gaggtgcgac agctattgga tagcggcatt cctcctgagg
     5341 atcttatctg gtatggtctc gaatacaaat tcgtgacaat gtatcttacc ggacaaatgg
     5401 agtacgaaga gatggta
//
LOCUS       DAOSIE010000085         5410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_13558_length_5410_cov_3.855649, whole genome shotgun sequence.
ACCESSION   DAOSIE010000085 DAOSIE010000000
VERSION     DAOSIE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13558_length_5410_cov_3.855649"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PLI69_03660"
     CDS             <1..1184
                     /locus_tag="PLI69_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG41618.1"
                     /translation="YALAHNRTLENASLDIKRAEATRWQTLASMLPQVTAGLDYTNMM
                     GYKLDFGAMVLAMPPSASFTVTTAVALSGAQIVSTQVANISKRMADISFLKSEQEITN
                     QVKVLYYSALVTEETLELLKENLESMRKLHSISQRSVDVGVSEQTAADQLKVQVAGME
                     SSINATERSLEMVYNSLRLQLNLDVNSEIELTQGISELMNVDAVSDLFREEFDVNNNY
                     NYKLLKAGTDLAKKQIALTGWSGGPTFSIFHQYAKKHYFSDEMTMNMTPPNMLGISVK
                     VPLFTSLRNTKSVQAAQISYQKQLNTLADTEAALKIQHRQLVYNLKSALEQYRTQKEN
                     VEVARRVFNNIALKYEHGVASALDVTNSGTSLIVAQNSYVQSLLQIVNAQISLEQLLN
                     R"
     gene            1191..2225
                     /locus_tag="PLI69_03665"
     CDS             1191..2225
                     /locus_tag="PLI69_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG41619.1"
                     /translation="MKKHMKIIRTSLIALVVMLLSSCGGSQQKQEEAVQERVEQVETT
                     TISRSVIRRVVDFSTTLMGYETVNIAPSITGKIEHIYVEVGSNVQKGSMLVRMDQTQL
                     NNTKLTHANLSVELQRMEALKESGAISRQVYDQTKLGYDQTAESLSFLEKNTFVKAPF
                     PGVISARNYEAGELYSGMPILVLTQINTLKALINIPESYFPLVKKGMKIDIKSDIYPG
                     ESFSGTIEIVYPTIDAATHSFQCKVRIPNSSLKLRPGMYVHTSLPLGHSEAIVVPYQT
                     ILKLVGSNERYIYLDKDGKAERVFVQTGQRFDDMIEIISDEVKEGDRLVSVGQAKLVD
                     GVKLNVVKQN"
     gene            2234..>5410
                     /locus_tag="PLI69_03670"
     CDS             2234..>5410
                     /locus_tag="PLI69_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG41620.1"
                     /translation="MSIYKTATRKPVTTILIFTAVIVIGLFSLNRLPIDQFPEIDPPF
                     VTVMTTYPGASAGEVETNVTKIIENSLNSIDGLKELTSSSRDNISIVSLEFEWGTNLD
                     EVSNDARSYLDMIRDNLPEGCSTPILFKLNTSMMPILQYYVLAKESYPGLEKILNDEV
                     VAILNRVDGIGTIGIAGAPQRYVYVDIDQAKLDAYGLPLELVGSAISSNNLNLASGTV
                     KMEKEQYHMQVRSEYIESAEIGDIVVTSVMGKPIFVRDIATIRDTIKDLTLDEKVNGQ
                     EAVRLIISKKSGANTVQVCKDVRKEIEKIQKNLPSDIEMGIIYDSSEEIQNSINSLKE
                     SILYALLFVVLVVFFFLGKWRATFIIAVTIPISLIVAFIYLLFVGSSLNIISLSSLTV
                     AIGMVVDDAIVVLENITKHIERGSSPREAAIYATNEVWVSVIATTLVIVAVFVPLTML
                     SGMAGIMFKELGWIVTIVISVSTIVAISLTPTLASQMLKANKVRIDEKGHLQVVKPKM
                     DWYERYIEKFHNKVSAWYANILRWSLRHKRATILIILIFFVASLFPVVTGKIGADFMP
                     ESDNGRISVSIELQRGTRLEETLKTARILEARFRELIPEIKIISTSAGSNDDAGIGAL
                     FYSTTNNKISMTVVCNKKYDRDRTIFEIAEVVRTELDKYPEIIDYQAATTIGMGMGGG
                     NKVSVEIYGHDFDDTNLYAQEVLQAVKTNIRGARDINISRDDDRPELKITVDKEKIAY
                     HGLNSAIVSTYVRNRVNGLLAGYLKEDGDEHEIIVRLKEEDRNSITLIENLTIPTNTG
                     RAIKLRELADIEEYWAPPTIDRKSRQRYLTLSITPFNISLGELAAQVQAEIDKISTPR
                     GVQVRLAGDYEEQQETFADMFQLLLLIVLLVYIVMASQFESLAKPAIIMMSVPFALSG
                     VILALWVTGINLDMIGALGFIMLVGIVVKNGIVLVDYTNLMRDRGYELNEAIALSGES
                     RLRPVLMTAVTTLLGMLPMALSRGEGSELWRPMGIVVIGGLLVSTVVTLIIVPILYAV
                     MSRHGERNKVEKLRKEFVFMQISADDEAA"
BASE COUNT         1525 a         1199 c         1299 g         1387 t
ORIGIN      
        1 agtacgctct tgcgcacaac cgcacccttg aaaatgcatc tctggatatc aagagggcag
       61 aagccacacg ttggcagaca cttgcaagca tgctacctca ggtgacggcg ggtttggatt
      121 ataccaatat gatgggttat aagttggatt tcggtgccat ggtacttgcc atgccgccca
      181 gcgcctcttt taccgttacc acagccgttg cattgagcgg agctcaaatt gtttccacgc
      241 aggtagccaa tatatcaaag cgtatggcgg acatctcttt tcttaagagc gaacaggaga
      301 ttacaaatca ggtaaaggta ctctattatt cggcgcttgt gacagaggag actctggagc
      361 tgctcaaaga aaatctcgag agtatgcgaa agctccacag catctctcaa aggtcggtgg
      421 atgttggggt atcagagcaa acggccgctg accagctcaa ggtgcaggtg gctggtatgg
      481 agagttccat caatgctaca gaacgttcgc tggagatggt atacaattct cttcgcctgc
      541 agctaaacct cgatgtcaac tcggaaatag aacttaccca gggtatttcg gaattgatga
      601 atgtggatgc ggtgagcgac cttttccgcg aggagtttga tgtaaataat aactataatt
      661 ataagttact taaagctggc acggatctcg caaaaaagca gattgcgctg acaggctggt
      721 cgggaggccc caccttcagc atttttcacc agtatgctaa aaagcactac ttcagtgatg
      781 agatgacgat gaatatgacg cctcccaata tgctcggaat ctcggttaaa gtacctttat
      841 ttaccagcct caggaatact aaatcggttc aggcagcgca aatctcatat caaaagcagc
      901 tcaatacgct tgccgatacc gaagcggctc tgaaaatcca gcacagacag ctggtctata
      961 acctaaagtc ggccctcgag cagtaccgga ctcagaagga aaacgtagag gtggcaagga
     1021 gagtgtttaa taatattgct ctcaagtatg agcacggagt agcctcagca ctggatgtga
     1081 ccaactcagg caccagtctg atagttgctc agaacagcta tgtacagtcc ctactacaga
     1141 ttgtgaacgc acagatctcc ctcgaacaac ttttgaacag ataaaaatca atgaaaaaac
     1201 atatgaaaat catccgtact tcactcattg ccctagtggt aatgctcctt tcaagttgtg
     1261 gcggatcaca acagaaacag gaggaggcag ttcaggaacg tgtagaacaa gtagagacta
     1321 ctaccatcag cagatcggta atccggcgag tggtggactt ttcaactact ctcatgggat
     1381 atgaaactgt aaatattgcc ccctctatca caggtaaaat agaacacatc tatgtggaag
     1441 taggttccaa tgtgcagaag ggttcgatgc ttgtgagaat ggatcagact caactcaaca
     1501 acaccaagct cacacatgcc aatctgtcag tagaacttca gcggatggaa gcactcaaag
     1561 agagcggtgc catttccagg caggtatatg accagactaa gctgggatat gaccagacag
     1621 cggaatctct ctcatttctt gaaaagaaca cctttgtcaa agcacctttc ccgggtgtca
     1681 tatcggccag gaactacgag gccggcgaac tctatagtgg tatgccgatt ctggtactta
     1741 cccagataaa caccctaaag gctttgatca atattccaga aagttatttt cctctggtaa
     1801 aaaagggaat gaagatcgac ataaaatcag acatctatcc cggagaatca ttctcgggta
     1861 caattgagat agtctatccc accatcgatg ctgccaccca tagctttcag tgcaaggtga
     1921 ggattcccaa tagttcttta aaactccgtc cgggaatgta tgtgcacact tctctacccc
     1981 ttggccactc tgaggccatt gtggtaccct atcagacaat tctgaagcta gtgggctcaa
     2041 atgagcgcta tatatatctc gataaagatg gtaaggctga aagggtattc gtacagaccg
     2101 gtcagcgttt tgacgatatg atagagatca tcagcgatga ggtaaaggag ggggaccgcc
     2161 tcgtctcagt gggacaggcc aaacttgttg acggagtcaa actcaatgtt gtaaagcaga
     2221 actaactgat actatgagta tatacaaaac agcgactaga aaaccggtga cgacaatact
     2281 gatcttcacc gccgtgatcg tcatagggct tttctccttg aacaggctgc ctattgacca
     2341 gtttccggag attgacccgc cattcgtaac ggttatgact acttatccgg gagcaagtgc
     2401 aggcgaggtt gagaccaacg taaccaagat tattgaaaac agcctcaact ctattgacgg
     2461 actcaaagaa cttacctcca gctcccggga caatatctcc attgtctctc tcgagtttga
     2521 atggggtaca aatttggacg aagtttccaa cgacgcgcgc tcttatctgg atatgataag
     2581 agacaatctt cccgagggtt gctcaacacc tatccttttc aaactcaata ccagcatgat
     2641 gccgatactc cagtattatg tgctggcaaa agagagttat cccggtcttg aaaagatcct
     2701 caatgatgag gttgtggcca tcctcaaccg tgttgatggg ataggcacca ttggtattgc
     2761 cggtgccccg cagcgttatg tatatgtgga tatagatcag gcaaaactgg atgcctacgg
     2821 ccttcctttg gaacttgtag gcagtgccat cagctcgaat aacctcaacc ttgcctccgg
     2881 taccgttaaa atggaaaaag aacagtatca catgcaggta cgcagcgaat atattgagag
     2941 tgctgagatt ggagatatag tggtgacctc tgttatgggt aagccgattt ttgtaaggga
     3001 tatcgccaca ataagggata ccatcaagga tcttacgctc gatgagaagg taaatggcca
     3061 ggaggcggtc aggttgataa tttcaaagaa atccggagcc aatacggtac aggtctgtaa
     3121 ggatgtacga aaagagattg aaaagatcca aaagaacctg ccctctgata tcgagatggg
     3181 tataatatat gactcttcgg aagaaattca gaactccatc aactcattga aggagtccat
     3241 tctttatgca ttgcttttcg tggtgcttgt ggtctttttc ttccttggaa aatggagagc
     3301 tacgtttatt atagccgtta ccatcccaat atcgctaatt gtagcattca tatatctgct
     3361 gttcgtaggt agctcgctca atatcatttc gctcagttcg ctgacggtag ctatcggcat
     3421 ggtggtggat gatgcaatag tcgtccttga aaatataacc aagcatatcg aacgcggctc
     3481 gtcacctcgt gaggcagcta tctatgctac aaatgaggtg tgggtttcgg ttattgccac
     3541 aacactcgtc atagttgccg tatttgttcc cttgaccatg ttgagcggta tggccggcat
     3601 catgttcaag gagctgggat ggatcgtgac catagtgatt tcggtctcca ccatagtggc
     3661 tatcagctta accccgactc tcgcttctca gatgcttaaa gccaataagg taagaattga
     3721 cgagaaggga catcttcagg ttgtaaaacc taaaatggac tggtacgaga gatatatcga
     3781 gaaattccac aataaggtaa gtgcttggta tgccaatatc ctgcgctggt ctctccgcca
     3841 caagagagcc actattctaa ttatattaat cttttttgtt gcctcccttt ttccggtggt
     3901 aaccggtaaa ataggagctg atttcatgcc cgaaagtgat aatgggcgca taagtgtcag
     3961 catcgaacta cagcgaggaa cccggcttga ggagactctc aagactgcac gtatacttga
     4021 ggccagattc cgcgaactca tacctgaaat taagataatt tccacttccg caggaagcaa
     4081 cgacgatgcc ggtattggcg ctcttttcta ctctaccacc aataataaga tctccatgac
     4141 tgtggtctgc aataagaaat atgatagaga taggacaata ttcgaaattg cagaggtggt
     4201 gcgtacagag ttggataaat atcctgaaat tattgactat caagctgcca ctaccatagg
     4261 aatgggaatg ggaggtggaa acaaagtaag tgtggaaata tatggtcacg acttcgatga
     4321 taccaatctc tatgcgcagg aggtactcca ggctgtaaag accaatatca ggggagcgcg
     4381 tgacatcaat ataagtcgtg atgatgatcg tcccgaactt aaaatcacag tcgataaaga
     4441 gaagatcgca tatcacggac tcaattcagc tatcgtctct acctatgttc gtaatcgtgt
     4501 caatggtctt ttggccggat acctcaaaga ggatggagat gagcatgaga tcatagtacg
     4561 tcttaaggag gaggatcgta actcgataac tctgattgaa aacttgacca ttccaacaaa
     4621 taccggtcgt gccatcaagc tcagagagtt ggctgatatt gaagagtatt gggccccgcc
     4681 gactatagac agaaagagcc gccagcgcta tctgacactc tccataactc cttttaatat
     4741 ctcattggga gaactcgccg cccaagtaca ggcggagatt gataaaattt ccactccgag
     4801 aggagtacag gtgaggcttg caggagacta tgaagagcag caggagacct ttgcggatat
     4861 gttccagttg ctactcttga ttgtcctttt ggtctatata gtgatggctt cgcaatttga
     4921 atcacttgcc aagcccgcca tcattatgat gtcagttcct ttcgcacttt cgggtgtcat
     4981 tttggcacta tgggttacgg gcatcaatct tgatatgata ggtgcgttgg gattcatcat
     5041 gcttgtgggc attgtggtca agaatggtat tgtgttggtt gactatacca atcttatgcg
     5101 cgaccgcggc tacgagctca acgaggcaat cgccctatca ggcgagtccc gtctccgccc
     5161 cgtgcttatg accgcggtga ctaccctact gggtatgctg cccatggcat tgagtagggg
     5221 tgaaggatcc gaactttggc gtccgatggg aattgtggtt ataggaggtc ttctggtctc
     5281 tacggttgtc actctgatca tagtgcctat cttatatgcg gtaatgtcgc gccacggtga
     5341 gcgcaacaaa gttgaaaagc tccgtaaaga gtttgttttt atgcaaatat cggctgacga
     5401 tgaggcagct
//
LOCUS       DAOSIE010000086         5260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_14032_length_5260_cov_4.802690, whole genome shotgun sequence.
ACCESSION   DAOSIE010000086 DAOSIE010000000
VERSION     DAOSIE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14032_length_5260_cov_4.802690"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLI69_03675"
     CDS             complement(<1..256)
                     /locus_tag="PLI69_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOG41621.1"
                     /translation="MYDYIRGALVELNPADVTIEAGGIGYHILISLTTYSSLQKSGKE
                     SVKLYLHQHLREDEELLFGFFDKEERTMFRHLISVSGIGPG"
     gene            335..1570
                     /locus_tag="PLI69_03680"
     CDS             335..1570
                     /locus_tag="PLI69_03680"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HOG41622.1"
                     /translation="MEIIEQIREEFPALHQQVWGKPLIYLDNAATTQKHRKVLELQER
                     VSARTNANIHRALHKLSADATDLYEAGREAVRIFLNAESSEEIIFTSGATAALNLIAS
                     SLVRSHLAKGDKVLVCEAEHHSNIVPWQLACEQYGLSLEVLPVEESGEISLERLIDLL
                     KADDRIRLLSVNHISNVLGIINPVAEIVRIAHSKGVLVVIDGAQGVVHDRVSVREMDC
                     DFYVFSGHKIYAPVGIGVLYGRRSLLENMPPYMGGGDMVDRVSFSRTTYAPLPLKFEA
                     GTPNFIAAACLKPALEFATAVRDDVQVREQEMKIVSTMVQELQQIEGLKIHGLPRDLS
                     LKVPIFSLSNEAVHPSDLAQMLDKMGIAVRSGFMCAEPLVSRFSELGLLRVSFAPYNT
                     FNECEIFISKLKLVTEMLK"
     gene            1567..2019
                     /locus_tag="PLI69_03685"
     CDS             1567..2019
                     /locus_tag="PLI69_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOG41623.1"
                     /translation="MIESKANIRSISEIEEEIVSEFTIFDEWLDKYEYLIELGKELEG
                     INESDKTEENLIRGCQSRVWLVCRNSGGRLWFGADSDAVITKGIISLLLKVYSGQKYD
                     DILASDFSFIERIGLKDNLSPTRANGLLSMIKQIKAYALAYKEKEVQQ"
     gene            2016..2333
                     /locus_tag="PLI69_03690"
     CDS             2016..2333
                     /locus_tag="PLI69_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOG41624.1"
                     /translation="MIEPKSDIERDIILALQQVFDPEIPVNIYELGLVYGLEVDKANN
                     VTVTMTLTSPNCPAADQLVEDVKTAVEDVPKVADVDVKIVFEPEWNMKMMSEAALLEL
                     GML"
     gene            2330..2803
                     /gene="folK"
                     /locus_tag="PLI69_03695"
     CDS             2330..2803
                     /gene="folK"
                     /locus_tag="PLI69_03695"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOG41625.1"
                     /translation="MIFLLLGSNLGDREANLSDARKRLCEALGFEGKESLQVYFSPEV
                     RTEAIGFEGAFFLNQIMAFEEKNITPRKLLKICQKIEIEMGREVHQAEFDEKGGRIYR
                     DRIIDMDILAFDDVRCNTKSLTLPHPQIWSRPFVMEIAALLPVPIFKLFQTIVNN"
     gene            2814..4352
                     /locus_tag="PLI69_03700"
     CDS             2814..4352
                     /locus_tag="PLI69_03700"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOG41626.1"
                     /translation="MTQEELFKTLTAHAKEYGFIFPSSEIYDGLGAVYDYGQMGVELK
                     NNIKKYWWEAMVHLNENIVGIDSAIFMHPKIWEASGHVGAFNDPLIDNRDSKKRYRAD
                     VLIEEYLAKLEERMNKEVEKGRRKFGEAFDEEQFRATNPNVLRNKVKWDEVHARMVKA
                     LNENDLAELKQIIIDSEIVCPISGTKNWTDVRQFNLMFSTQLGTISEEAGTIYLRPET
                     AQGIFVNFLNVQKSARMKVPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRPG
                     SELEWFEKWKEARLAWHKALGLGDEKYRYHDHEKLAHYANAATDIEFEFPFGFKELEG
                     IHSRTDFDLAQHQIFTGRKMQYFDPETGESYVPYVVETSIGLDRTFLAILSAAYSEEE
                     LENGESRVVLRIPPAIAPVKLAVLPLVKKDALPEKAREIMNELKYDFNCQYDEKDSIG
                     RRYRRQDAIGTPFCITIDHDTLEDNRVTIRHRDTMEQERVPIASLRKIMEEKVSLRSL
                     LEKL"
     gene            4564..>5260
                     /gene="rhuM"
                     /locus_tag="PLI69_03705"
     CDS             4564..>5260
                     /gene="rhuM"
                     /locus_tag="PLI69_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOG41627.1"
                     /translation="MKKKHEITKKKETDIRSSAAEYLTFVAANGDNYQSIEIRYEEEN
                     IWLTQKMMATLYDVSIPAINQHLKTIFEDNELQEDAVVKEYLITAEDGKNYKTKHYNL
                     QAIISVGFKINNRQAVQFRKWANGVVKDYTIQGWVMDVDRLKNFGTILTKDYFEKQLE
                     VIREIRLSERRFYQKITDIYATSLDYDPNSKITLDFYKKVQNKLHWAIHGHTAAELIY
                     KRADAEASNMGLQT"
BASE COUNT         1521 a         1034 c         1312 g         1393 t
ORIGIN      
        1 taccgggacc gatgccgctt actgagatca gatgacggaa catggtgcgc tcctccttgt
       61 caaaaaagcc gaaaaggagc tcttcatctt cacggagatg ttggtggaga tagagtttaa
      121 cgctctcctt gccacttttc tgaaggctag agtaggtggt aagcgaaatt agtatgtggt
      181 agccgatgcc accggcctca atagtcacat ccgcgggatt aagctcgact agcgctcctc
      241 tgatataatc gtacattcta tctttgttta attttctcgt aaaaatatgc tttttaggcc
      301 aaaaaccaaa gtttgtttag tacttttgca agttatggag attatagaac agatacgcga
      361 agaatttccg gcgctgcacc agcaggtctg gggcaagccg ctcatatatc tggacaatgc
      421 cgctaccacg cagaagcacc gcaaggtact ggaattgcag gaacgtgtga gtgcgcgcac
      481 caatgccaat atccatcgtg cactgcataa gctgagcgct gatgcgactg atctctatga
      541 agcgggtcgt gaggcggtac gcattttttt gaacgcggag agcagtgagg agataatctt
      601 cacatccggg gcaactgccg ccctcaatct gatagcctct tcgctcgttc gcagccattt
      661 ggcaaagggt gacaaagtgc tggtttgtga agctgaacat cactcaaata tagtcccctg
      721 gcagcttgct tgcgagcaat acggcctatc gttggaggta ttgccggtgg aggagagtgg
      781 tgagatatct ttggagcggc ttattgatct gcttaaggct gacgatcgta tccgtttgct
      841 ctctgtcaat catatctcca acgtgttggg aattataaat ccggtagctg aaatcgtgcg
      901 tatagctcat tccaaaggag tattggttgt tattgacggt gctcagggag tggtacacga
      961 cagagtctct gttcgggaga tggattgcga cttttatgta ttctcaggac ataaaatata
     1021 tgcacctgtc ggtatcggag ttctatacgg acgacgctcg cttctcgaga acatgccccc
     1081 ctatatggga gggggcgata tggtggatag ggtgagcttc agtcgcacca cttatgctcc
     1141 gcttccgtta aaatttgagg cgggtactcc gaattttata gctgcggctt gtctcaaacc
     1201 ggcactggaa tttgcaactg ccgtaaggga tgatgtgcag gtacgggagc aggaaatgaa
     1261 gattgtgagt actatggtgc aggaattgca gcagatagag ggacttaaga tacacggttt
     1321 gccacgggat ctctccttaa aagtgcccat tttctctcta agtaatgagg cagttcatcc
     1381 atccgatctc gcacagatgc tcgacaagat gggtatagcg gtacgctccg gattcatgtg
     1441 tgcagaaccg cttgtgagcc gtttttcgga gctaggattg ctgagagtat cttttgcacc
     1501 atacaacact tttaatgagt gtgaaatatt tataagcaag ttgaaacttg taacagagat
     1561 gttaaaatga ttgaatcaaa ggcaaatata aggagcataa gcgaaattga ggaggaaatt
     1621 gtttccgaat tcacgatttt tgatgaatgg ctcgacaaat atgagtatct gattgagttg
     1681 ggtaaagagt tggaaggcat caatgagagt gataaaacgg aagagaacct tataagggga
     1741 tgccaatctc gtgtctggct tgtttgccga aacagcggag ggcgcctctg gtttggtgca
     1801 gatagcgacg ccgtcatcac caaaggcata atctcactct tgttgaaggt ctatagcggc
     1861 cagaaatatg atgatatcct ggcttccgat ttttcattca tagaaaggat cgggcttaag
     1921 gacaatctct ccccgactcg tgccaatggc cttttatcaa tgataaaaca gattaaagct
     1981 tatgctttgg catacaaaga aaaggaggta caacaatgat agaacccaaa agcgatatag
     2041 agagggatat aatccttgcg ttacaacagg tatttgaccc ggaaattccg gttaatatct
     2101 acgaacttgg ccttgtttac ggacttgagg tggataaagc aaacaatgtc actgtgacga
     2161 tgacccttac ttcacccaat tgtcctgcgg ctgatcagct tgtggaagat gtcaaaacgg
     2221 ctgtcgagga tgttcccaag gtggcagatg tggacgttaa aatagtcttt gagcccgaat
     2281 ggaacatgaa gatgatgagt gaggcggctc ttttggagct ggggatgttg tgattttcct
     2341 gctattggga agcaatttgg gcgacaggga ggcaaacctt tccgatgctc gtaaaagatt
     2401 atgtgaggcg cttggatttg agggaaaaga gtcgttacag gtctactttt cacctgaagt
     2461 gagaactgag gccatcggat ttgaaggtgc gtttttccta aaccagatta tggcttttga
     2521 ggagaagaat atcactcccc gaaaattgct gaagatttgt cagaagattg agattgaaat
     2581 ggggcgtgag gtgcatcagg cagagttcga tgagaaggga ggaagaatat accgtgaccg
     2641 gattatagat atggatattc ttgccttcga tgatgtgcgg tgcaatacga aaagtttgac
     2701 attgccccat ccgcagattt ggagcaggcc ctttgtgatg gagatagcgg cattgctgcc
     2761 ggtacctatt tttaaacttt ttcaaacaat tgtaaataat tagaaacgaa tatatgacac
     2821 aagaagaact ttttaagacc ctgacggctc acgctaaaga gtatggattc attttccctt
     2881 ccagcgaaat ttatgatggt cttggtgccg tatatgatta cggacagatg ggagttgaac
     2941 ttaagaataa tatcaagaaa tattggtggg aagcgatggt ccacctcaac gaaaatattg
     3001 tagggattga ttcggcaatc ttcatgcacc ccaaaatttg ggaggcatcg ggtcatgtgg
     3061 gggcattcaa tgatcctctg atagacaata gggactctaa aaagcgctat cgtgccgatg
     3121 tgttgattga ggagtatctg gccaagcttg aggagaggat gaacaaagag gtggaaaaag
     3181 gtcgccgcaa atttggcgaa gcctttgatg aggaacaatt cagggcaacc aatcccaatg
     3241 ttctccgcaa caaggtaaaa tgggacgaag tgcacgccag gatggttaag gcactcaatg
     3301 aaaacgatct tgccgaactt aaacagatta tcattgattc tgagattgtt tgtcctattt
     3361 ccggcactaa aaattggact gacgtacgtc agttcaatct tatgttcagc actcagctgg
     3421 gtaccatatc cgaagaggcc ggcactatat accttcgtcc tgagacggca cagggtattt
     3481 ttgttaactt tctgaatgtt cagaagagcg ccaggatgaa ggttcccttc ggaatagcgc
     3541 agatagggaa agctttccgc aatgagattg ttgccagaca atttattttc cgtatgaggg
     3601 agtttgagca gatggagatg cagtttttcg tgcgtccggg cagcgaactt gaatggttcg
     3661 aaaaatggaa agaggctcgt cttgcctggc acaaggccct gggacttggt gacgagaaat
     3721 atcgctacca cgatcacgag aagctggcgc actacgccaa cgcagccaca gatatagagt
     3781 ttgaattccc cttcggtttc aaggagctgg agggtatcca ctcccgtacc gatttcgacc
     3841 ttgcgcagca tcagatattc accggacgca aaatgcaata tttcgatccc gagaccggcg
     3901 aaagctacgt gccttatgtg gttgagacct ctattggact cgacaggacc ttccttgcga
     3961 tcctttcggc agcttactca gaagaggagc ttgagaatgg agagtcgagg gtagtgttgc
     4021 gcattccgcc tgccatagct ccggtcaaac tggccgtatt gcctcttgtc aagaaagatg
     4081 ctctccctga gaaggcccgc gagataatga atgagcttaa gtacgatttt aattgccagt
     4141 acgacgaaaa agatagcatc ggcagacgct atcgccgtca ggatgccatc ggcaccccct
     4201 tctgtatcac aatcgaccat gataccctgg aggacaaccg cgtcaccatc cgccatcgcg
     4261 atactatgga gcaggagagg gttcccatcg cttcactccg caaaataatg gaggaaaaag
     4321 tgagtttgcg cagtttactc gagaaactgt agaaaagaag tggtggttcg gtatttccga
     4381 acacccactc ggaatgaata tgaaaaacag ctctaggtgt atgcctggcg ctgttttttc
     4441 aattttagat gttaaaattg tcttaattgc agtagtgata aatttgtttt tcttatattt
     4501 ggaaaataac aaacaacttt atgtcattag atttattgtt atgtaattag aaatagaggc
     4561 cgtatgaaaa aaaaacatga aataacaaag aaaaaagaaa ctgatataag aagttcagct
     4621 gccgagtatc ttacctttgt tgctgctaac ggtgataatt atcaaagcat tgaaattcgg
     4681 tacgaagaag agaatatttg gctgacacag aagatgatgg ctacattgta cgatgtttca
     4741 attccggcta taaatcaaca tttgaaaaca atttttgaag ataatgaatt gcaggaagat
     4801 gcagttgtta aggaatactt aataactgcc gaagatggta aaaattataa aacaaaacac
     4861 tataatcttc aagcaatcat ttccgttgga tttaaaatca ataaccggca agccgttcag
     4921 tttcgcaagt gggccaatgg agttgtaaaa gattacacca tacaagggtg ggtcatggat
     4981 gttgataggc tcaaaaactt tggaacgatt cttacgaaag attattttga aaaacaactc
     5041 gaagttattc gggaaataag actttctgaa agacgctttt accaaaaaat tacagatatt
     5101 tatgccactt ccctcgatta tgatccaaat tccaaaatca ccttggactt ctataaaaag
     5161 gttcaaaata aattacactg ggcaattcac ggacatactg ccgctgagct gatttataaa
     5221 cgggctgatg cagaagcaag taacatggga cttcaaactt
//
LOCUS       DAOSIE010000087         5214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_14185_length_5214_cov_4.862376, whole genome shotgun sequence.
ACCESSION   DAOSIE010000087 DAOSIE010000000
VERSION     DAOSIE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14185_length_5214_cov_4.862376"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLI69_03710"
     CDS             complement(<1..785)
                     /locus_tag="PLI69_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41628.1"
                     /translation="MKILSKKVNPASGKSEIILSMRYGRDKVLRARTHLLIAPKYFDL
                     QREGHVVLGEIVVKSKIHSPEVIQAKEVRNKIEDMCRLFEDRIAGENPENITLDWLQK
                     QVDQFIYGDTSSEVVEISKPKSFFDHFDDYLAVSQFSVSRRKHFLVLYRALQRYALYR
                     NYEISFGTFNPAIIADFERFLRNEHTFFVEDKKASGKNTKQGGANRIPVKKYRAVFDK
                     FPECRIPKQRGDNYIKGLLIILRTFFLWANKTGKTNCNPFRER"
     gene            complement(1108..1686)
                     /locus_tag="PLI69_03715"
     CDS             complement(1108..1686)
                     /locus_tag="PLI69_03715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOG41629.1"
                     /translation="MKKLIVIDSCMRAESRTKLILDAAVAELSKRYEVEIIDVNSVKL
                     SPLDKITYVEQRENGNIPPLAVELARKVAACDRLVVAAPFWDMSFPAILKSFFENISL
                     FDITFTDDGTTCVGLCKCEKVLFITTRGMDIKTGDPLDQGSPYLAALSFLWGLGEVIT
                     VAATNMDYLSPEELVLRIEAAKNEVVEICKEF"
     gene            complement(1700..2002)
                     /locus_tag="PLI69_03720"
     CDS             complement(1700..2002)
                     /locus_tag="PLI69_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HOG41630.1"
                     /translation="MKRIRITAVRKVEHKDLIEKYENPIEHPCDIEEGDVWIAEGWER
                     PEGFCESAWDSVSPFVMALSHGATGFYDGWMKNPASAMISCNDGFRPVSFLIEVID"
     gene            complement(2062..2134)
                     /locus_tag="PLI69_03725"
     tRNA            complement(2062..2134)
                     /locus_tag="PLI69_03725"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2099..2101),aa:Ala,seq:ggc)
     gene            2243..5026
                     /locus_tag="PLI69_03730"
     CDS             2243..5026
                     /locus_tag="PLI69_03730"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HOG41631.1"
                     /translation="MSEEKRKNYGYLCADSTEVINGKGRNIIPEPESEPLWKAYLAKF
                     RDPIIIILLVALGLSLCISLYEMLVMGRSASCLIEPLGVLIAILLATGIAFIFEYNAE
                     KEFKILNKAKDERKVKVIRWRSAHEAKDDAWPQVYEIPRGDVCLGDIVKLESGDEIPA
                     DGILLEALSLRIDESNFTGEPFTRKYVEIDPTRPESTYPQNFLLRGTTLIEGTALYRV
                     TAVGPETEEGKGAQILQEESTVETPLNNQLNRLAQSITKASYILGTLVVVGRLLIFFL
                     TGDSGERELLDIIHVIINSVMLAVTLIVVTVPEGLPMSVTISLALSMRKMLKEKNLVR
                     KLHACETMGATTVICTDKTGTLTYNKMSVVEQEFSGVDMDFVALSISINSTAELSEIE
                     SGKLRAIGNPTEGALLLWLHDLGYNYHKLRESVLITSQEPFSTESKRMITVAKDEGTG
                     RSVRYIKGAPEIVLEMSDSIAGKRNKEEILDLLQSYQAKGMRTLGFAFQELNAEEDTP
                     VIFAGIVGIADPVRDDVREAIEMSRNNAGVRVIMITGDVALTANEISRQSGIMDAHEP
                     MQTLTGAEFALMSDEEIKTRVIPELKVLSRARPEDKARLVTLLQEMGEVVAVTGDGTN
                     DAPALKKAQVGLSMGDGTSRAKEASDVTIIDNSFSSINKGILWGRSLYLNIKRFILFQ
                     MTINACASLIVLLGAFLGLDSPLTVTQMLWVNLIMDTFAAMALSSIPPDRRVMNAPPR
                     DSKSHIIDRKMGARIIIGGFIFFIVMTLIWQLLWHTDITSVKDLLNAETLRELFTGVF
                     RSHTKNHLSGFERGVFFSIFVMLQFWNLFNARYFRTNGSALQDILDLFFNRKRFRESA
                     SLTLLMVAGIILLGQYFITNVAGAFFDVAPLSWGDWGLILLCTSPVLLIPDIWRFVRN
                     IFSASREKKYARI"
BASE COUNT         1495 a         1172 c         1175 g         1372 t
ORIGIN      
        1 tgacgctccc tgaatggatt gcaatttgtc ttgcctgtct tgtttgccca aagaaagaat
       61 gtccttagaa tgataagcag tccttttata tagttatccc ctctttgctt gggtatcctg
      121 cattcaggaa atttatcgaa gacggctctg tattttttta ccgggatcct gttggcccca
      181 ccctgcttcg tgtttttccc ggaagccttc ttatcctcaa cgaagaatgt atgttcattg
      241 cgaagaaacc gttcaaaatc ggcaataatg gcgggattga atgtaccaaa agaaatttca
      301 taattcctgt aaagcgcata acgttgtagg gcacggtaca ggaccagaaa atgttttctt
      361 ctgcttaccg agaattggga aacagccagg taatcatcaa aatgatcaaa gaaagatttt
      421 ggcttactta tctcaacaac ttcagagctg gtatcaccgt aaatgaattg atcgacctgt
      481 ttctgcagcc agtcaagagt tatattttcc gggttttctc ctgcaatacg atcttcgaat
      541 aaacggcaca tatcctcaat tttattcctc acctcttttg cctggataac ttcaggtgaa
      601 tgaatctttg atttaactac tatctcgcca agtacaacat gaccttctct ttggagatca
      661 aaatatttag gtgcaattaa caaatgagtt cttgcacgga gtactttgtc gcgaccgtag
      721 cgcatgctca atattatttc tgattttccg gaagcggggt tgaccttctt ggatagaatt
      781 ttcaagacct ttggcatgac gataattttt aagcatcaca aatataaata atgcaggcca
      841 gaattcataa aattttctga tgaattcacg accaaaatgt tccgcaaata ttccgctaaa
      901 tctaaaaagt aaaaaaagga gagaaataag ctgaaaaaaa acgaataact tatttcattg
      961 ttttccaatt ctttacattg tgtaatcgac tatataccag aatatttaaa aagagataaa
     1021 tagatcttgg cgagtcttgt cctgggcact aaaagaggga ggaactaaaa agtcctccct
     1081 cttttagtgc aggtcgcttc gctccactca aaactcctta catatctcta ctacctcgtt
     1141 tttagcagct tcaatacgca aaacaagttc ttcaggagaa aggtagtcca tatttgtggc
     1201 ggcgacggta atcacctcgc cgagacccca aagaaatgat agggcggcca agtatggcga
     1261 accttggtca agcggatctc ctgtctttat atccatgccg cgtgtggtga taaagagcac
     1321 tttttcgcat ttacacagcc ctacacaggt agtcccgtcg tccgtaaaag ttatatcgaa
     1381 cagcgatata ttttcgaaaa atgacttgag aattgcggga aaactcatat cccaaaaggg
     1441 agcggcaaca accagacggt cacaggcagc cactttgcgc gctagttcta ctgccagagg
     1501 cggaatgttc ccgttttctc gttgttcaac ataagtgatt ttgtccaacg gagacaattt
     1561 tacagaattg acatcaatga tctccacctc atatctcttg gagagttcag ccacagcggc
     1621 atcgaggatc aattttgttc tggattcggc cctcatgcaa gagtctatga caatcaattt
     1681 tttcatattt atcggtattt cagtctatca cttctataag aaaactcacg ggacggaaac
     1741 cgtcgttgca cgagatcatc gctgaggcag ggtttttcat ccatccatcg taaaagccgg
     1801 tagcaccgtg tgaaagtgcc atcacaaagg gagaaacgga gtcccatgcg ctctcacaaa
     1861 acccctcagg cctttcccaa ccctctgcaa tccagacatc accctcctca atatcacagg
     1921 ggtgctcaat gggattttcg tacttctcta taaggtcctt gtgttcaact ttgcgaacgg
     1981 cggtgatgcg tattcttttc ataaggaagg tgttttccgt tatcacaaaa aagccccaca
     2041 ttgctgcggg gctaaatggt gtggagtata ggagaatcga actcctgacc ttttgaatgc
     2101 cattcaaacg ctctaccaac tgagctaata ccccattgga accgcaaaag taagcaaaag
     2161 tattggaaat acaactattt tttgacagcc tcggcaaaaa aatggtaact tcgctttttc
     2221 aataaattga tccacttgac aaatgtcaga agaaaaaaga aaaaactatg gatacctttg
     2281 tgcggattct acggaggtaa ttaacggaaa aggtcgcaat atcatccctg aaccggagag
     2341 cgaaccctta tggaaggcct atctggccaa gttcagggac cccattatca taatactgct
     2401 ggtagcactc ggcctctcgc tctgtatatc cttatatgag atgttggtaa tgggaaggtc
     2461 cgcctcctgc ctgattgaac ctctcggagt gctcattgct atattgcttg ccaccggaat
     2521 agcgtttatc tttgagtaca acgccgaaaa ggagttcaag attctgaata aagccaaaga
     2581 cgaacgtaaa gtcaaggtaa ttaggtggcg tagcgcacac gaagcgaaag atgatgcatg
     2641 gccgcaggtt tacgagattc caagaggcga tgtctgtctc ggggacattg tcaaattgga
     2701 gagtggtgat gagattcccg ccgacggaat cctgctggaa gcgctctctc tgcgcattga
     2761 cgaatccaat tttaccggag aaccgtttac cagaaaatat gtggagattg accctacgcg
     2821 accggagagc acctatcccc agaattttct cctaagaggt actaccctta tcgaaggcac
     2881 tgcactctat agggtgactg cagtgggacc tgaaaccgaa gagggcaagg gtgctcaaat
     2941 tctccaggag gagagcaccg tcgaaactcc tctcaataat cagctcaatc gtcttgcaca
     3001 atcgattacc aaggcgagtt atatactcgg tacgcttgta gttgtcggac gtctgctcat
     3061 atttttcctg accggagatt ccggagagcg cgaattactg gatatcatcc atgtcataat
     3121 aaactcagtg atgcttgcgg taactctgat agttgtaaca gtacctgagg gactcccgat
     3181 gagtgttacc atttcacttg cgctgagtat gcgcaaaatg ttgaaggaga aaaatctggt
     3241 acgtaagctt cacgcctgcg agactatggg agcaacgact gtcatctgca ccgacaagac
     3301 cggcacgctc acctacaaca agatgagtgt ggtagaacag gaattctcag gtgtggatat
     3361 ggattttgta gccctgagca tctccatcaa ctccactgca gagctatctg agatcgaatc
     3421 aggcaagctc cgtgcaatag gcaatcctac tgagggcgca ttgctcctct ggctgcacga
     3481 tctggggtac aactaccata agctacgcga aagtgtgttg ataacctctc aagaaccttt
     3541 ctctacagag agtaaaagga tgattactgt tgcaaaagat gagggtacag gcaggagcgt
     3601 gcgttatata aaaggtgcac cggaaatcgt actggaaatg tcggatagca tagccggtaa
     3661 gcgtaataaa gaggagattc tcgacctcct tcagagttat caggcaaaag ggatgcgtac
     3721 actcggtttt gcatttcagg aactgaatgc tgaagaagac actcctgtaa tatttgccgg
     3781 tatagtcggc atcgcagatc cggtaaggga tgatgtgcgg gaagctattg agatgagtag
     3841 aaacaatgcc ggcgtcagag tgattatgat taccggagat gtagccttga cggcaaatga
     3901 gatttcgcgt cagtccggaa taatggatgc tcatgagccg atgcagaccc taacaggggc
     3961 agaatttgct ctaatgagcg acgaagagat aaaaactcgt gtcataccgg aacttaaagt
     4021 cctctccagg gcacggccgg aagacaaagc acgactggta actctgctac aagagatggg
     4081 agaagtggtg gcagtaacag gtgacggcac aaatgacgcc cctgcactca aaaaggccca
     4141 ggtaggactc tcaatgggcg acggcacctc cagagcaaag gaggccagcg atgtcacaat
     4201 catagataac tccttttcca gcatcaataa aggtattcta tggggacgct cgctctatct
     4261 caacataaag cgcttcattc tcttccagat gaccattaat gcgtgtgcca gccttatagt
     4321 tttgctggga gcctttctcg ggctggactc accccttact gtcacgcaga tgctttgggt
     4381 caatctaatc atggacacat ttgcagccat ggcgctctcc tccatccccc ccgatcgcag
     4441 agtgatgaat gccccgccaa gagattccaa aagccatata atcgacagaa agatgggtgc
     4501 acgcatcatt atcggaggct ttattttctt tattgtgatg acccttatct ggcaattgct
     4561 ttggcacacc gatatcactt cagttaaaga tttgctcaat gcggagactc tacgagaact
     4621 ctttacggga gtattcagat cacacaccaa gaatcacctt tcaggctttg agaggggcgt
     4681 cttcttctcc atcttcgtga tgctgcaatt ctggaaccta ttcaatgcaa ggtattttcg
     4741 cactaatgga agtgcattgc aggatatcct cgatttgttt ttcaatcgca aacgtttcag
     4801 ggagtccgcc tctctgactc tgttgatggt tgcaggcatt attttgctcg ggcagtactt
     4861 tataaccaac gttgcgggag cctttttcga tgtagccccg ctctcatggg gtgactgggg
     4921 gctgatactg ctctgcacct ctcccgtact gcttatcccg gacatttgga ggtttgtcag
     4981 aaacatattt tccgcttcgc gggaaaagaa atacgcaaga atataataga gaatgagata
     5041 ctaaacggtc ttcaatagag aaggatctgt caaaggtacg cgaagataaa tgaacaggaa
     5101 taattgagga ttggaattaa accaagagaa ttttcataaa aatgcaatac aacgagacaa
     5161 taaccggagt tctgaggttc ctcgaaaatg caggaataga gtacaaaata caca
//
LOCUS       DAOSIE010000088         5170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_14354_length_5170_cov_4.586119, whole genome shotgun sequence.
ACCESSION   DAOSIE010000088 DAOSIE010000000
VERSION     DAOSIE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14354_length_5170_cov_4.586119"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1056)
                     /locus_tag="PLI69_03735"
     CDS             complement(100..1056)
                     /locus_tag="PLI69_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG41632.1"
                     /translation="MKRILVVGAGGQIGTELVPHLQKNYGKDNVIASDLKDEMVEKLR
                     GFVTTEQFDALDEQRFAEVVKQYKIDTIFNLVAMLSAAGEARPLTAWRLNMGALLNSL
                     NIAKECECAVFTPSSIGVFGESTPHNMTPQDTIMRPNTIYGVCKVAGELLSDYYFTRF
                     GVDTRSVRYPGIISSGALPGGGTTDYAVEVFYEVLKNGRYTCEVPEETYMDMLYMPDA
                     LDAVIQLMEADPDKLIHRNSFNITSMSFNPRELFASISKRLPNAVFDYNIDPVKAAIS
                     ASWPDKMDDSCARAEWGWNPKWDMESMVDDMLAAIGKKLGIS"
     gene            1106..2002
                     /gene="queG"
                     /locus_tag="PLI69_03740"
     CDS             1106..2002
                     /gene="queG"
                     /locus_tag="PLI69_03740"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOG41633.1"
                     /translation="MSILISISSLSNKEGFVDWGFAPVAETGEDYSRFREYISRGENA
                     DMKYLANNMDKRENPSLLVPGARSVICFLAPYSSIQSSIAAFALGKDYHDVVKEKLRR
                     ISLHLSQECAKHGLEYQGRCFTDSAPVMERYWAAEAGLGFIGRNNFLISPKHGLRTLI
                     GVIICNIPPDWLGERREKIADGCGECRRCMDACPEGALYAPFRLDARKCISYHTIESH
                     ELYSAHPVEYKGQIFGCEICLRACPYDRESEGWEELRTNAEEILSKSPQDWHDMDEKE
                     FKERFGDTPLSRAGLDKIKNNL"
     gene            1999..2856
                     /locus_tag="PLI69_03745"
     CDS             1999..2856
                     /locus_tag="PLI69_03745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOG41634.1"
                     /translation="MMILQTPVEYTPLKERLGYKNKYLFIGSCFAAEIGSMMKDMGFD
                     LLLNPFGVLYNPASIHSSINRLASGTPFTEKDIITSDGRYTSFFHHSTFTRESAEEFL
                     TNANACLERDSARFAAADTCVVTLGTAWVFRHLQRDIIVSNCHKIHPAQFRRERLETE
                     EVTDLLCEIVEAHPDKKWIFTVSPIRHLKDTAHGNQLSKSILLLAIDRLQQLHPQVEY
                     FPAYEIMMDELRDYRFYAENMTHPTNQSIRYIFDRFCDYAIDDSEMATIAEAQKRLKA
                     SRHISFTSK"
     gene            complement(2843..>5170)
                     /locus_tag="PLI69_03750"
     CDS             complement(2843..>5170)
                     /locus_tag="PLI69_03750"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOG41635.1"
                     /translation="SFRDCTGPQTKEKFTITGAPDAEYVVSIFDKTTDRYGANSFSFS
                     PLSVKVHNAAPYIKTTLAYRYQPLYGYRSDTKDMVARSSARDMTNYIVESMPLVSNVE
                     EESSVEEKEASGSEVMTIRSQFGELIAFYPHLRSGRDGKVEVEYTTGDLLSTFRVLVM
                     GYDQSLKTGNAERSLIVRKELMVVPNIPLFIREGDKIVIKSKVVNLSEEQLKGNGYIK
                     FYNEETGEELILKDTDVKALTLAVGSQKELAWSVTPPENVSKLGVAISFKAGNYSDGE
                     KHIVTILPKEITITEAASFVIGGSHGHKYYEDQLHKRIKARNPRIRHVEYSTLTAVKE
                     ALPVVSKPESNNAIEWTNALYINQMRAKVLTMDGREKEVDGALVESFREEAIKALSKL
                     HNIDGGFAWFDGMPSSERLTLFFLEKMAQLRDFSAIEFSEAEKKLVEGAVGYIDERIR
                     EAYSRERYNSFNSINLFYVRSHYLEYQMKGKVSTAFSKFLSDTADGWQKIAILNKAQL
                     AHILLNCKETGFWSKKFDSRIADLNASLKDYAVINPTIGCYFPNAVMPFRGLMNSEIY
                     AHAKLMTLFARLGEKEMVDSLALWMLLQKHNQAWENTVATTDAVHALVSSGAKDLKLG
                     AVYYTYDTIIDDVKPAANEIEVNREFVRASDSKVITDGDKLNVGDEIIARYYIRNSEN
                     RSFVRMKAMRPACFYPLDERSFFFTGFWGEFYKEQHESLTNYYFELLPEGNLTIEERF
                     YITQEGTFSTSLVEIESLYANEYRGHTGSVKIISK"
BASE COUNT         1329 a         1241 c         1195 g         1405 t
ORIGIN      
        1 aaaagattta aggaactgcc accaatacat aaagaagcat ttttgaaagg tatttttaaa
       61 acagaaaaag cttgttataa acaggagttt atagcttact tacgaaatcc ctaatttttt
      121 gccgatggcg gcaagcatat cgtcaaccat tgattccata tcccatttgg ggttccaacc
      181 ccattctgca cgggcacaag agtcatccat cttatcaggc catgaagcgg aaattgccgc
      241 tttgacaggg tctatattat agtcgaaaac ggcattagga agacgtttgg atatcgatgc
      301 gaagagttcg cggggattga aactcatcga agtgatgttg aaactgttgc ggtggatcaa
      361 tttgtcggga tcggcctcca taagttgtat aactgcatcc agagcatcag gcatatagag
      421 catatccata taggtctctt ccggcacctc gcaggtgtaa cggccgtttt tcagaacttc
      481 gtaaaatacc tcaacggcat aatccgtagt accgccgccg ggcagcgctc cgctagagat
      541 aatgcccgga taacgtacac tgcgtgtatc aactccgaaa cgggtaaaat agtagtcgct
      601 gagcaactca cccgcaacct tacacacacc ataaatggta ttgggtctca tgatagtatc
      661 ctgcggagtc atgttgtgag gtgtgctttc gccgaatact cctattgagc tcggagtgaa
      721 taccgcgcat tcacattctt tagcaatatt gagtgagttg agcagggctc ccatattgag
      781 acgccaggct gtcaaaggtc tggcctcccc ggccgcactt agcatagcca caagattgaa
      841 tatggtatct atcttgtatt gctttacaac ctccgcaaaa cgctgctcgt cgagggcgtc
      901 aaattgttcg gtagtcacga agccgcgcaa cttttcgacc atctcatctt tcaaatcaga
      961 agcaattaca ttatctttac catagttttt ctggaggtgg ggtacgagtt cggtacctat
     1021 ttgacctccg gcacctacaa caagtatgcg tttcatatca atttttaacg aaattattat
     1081 acaaatataa tgattttaca gtaaaatgag cattttaatt tcgatttcgt cattatccaa
     1141 taaagagggg ttcgttgact ggggttttgc tccggtagca gagactggag aagattatag
     1201 tcgcttcagg gaatatattt cccgcggaga aaatgccgat atgaaatatc ttgcaaacaa
     1261 tatggataaa agggagaatc cttccctatt ggttccgggt gcccgttcgg tgatctgttt
     1321 tctggccccc tacagcagta tccaaagctc catagctgca tttgccctcg gaaaggatta
     1381 ccacgatgtg gtaaaggaga agctccggcg gatctcactg catctgtcgc aagaatgcgc
     1441 taagcatggt ctggagtatc agggacgctg ttttaccgac agtgctccgg taatggagcg
     1501 ctattgggcc gctgaggcgg gattgggttt tatcgggcgc aataatttcc ttatatcgcc
     1561 gaagcacggg ctgcgtacac tgatcggagt gatcatctgc aatatcccgc cggattggct
     1621 cggagagcga agggagaaga tagcagatgg gtgtggggag tgccgacgct gtatggatgc
     1681 atgtcccgaa ggtgcgctat atgccccttt ccggctggat gcgcgcaaat gtatctccta
     1741 ccacactatt gagtcacatg agctttattc cgcccaccct gtagaataca aaggtcaaat
     1801 cttcggctgc gagatctgtc tccgcgcctg cccttatgac cgcgagtccg agggatggga
     1861 agagttgcgc accaatgcgg aggagatact atccaagagt ccgcaagatt ggcacgatat
     1921 ggatgaaaaa gaatttaagg aacggtttgg cgatacgcct ttaagccgag ccggccttga
     1981 caaaataaag aacaatttat gatgatatta cagaccccgg tagagtacac tcccctgaaa
     2041 gagcggctcg gatataagaa caaatattta tttatcggct cctgctttgc agccgagatc
     2101 ggctctatga tgaaggatat gggatttgat ctattgctga accccttcgg tgtgctctat
     2161 aaccccgctt ccatccattc ttccataaat aggctcgctt cgggaactcc tttcactgaa
     2221 aaggatatta tcaccagcga cgggcgctac acctcatttt tccatcacag cacctttaca
     2281 agggagagtg cagaggagtt tctaaccaat gccaatgcct gcctggagag ggacagcgca
     2341 cggtttgctg ctgccgatac ctgcgtagtg actctcggaa ctgcatgggt gttcagacat
     2401 ttgcagcgcg atataattgt ttccaattgc cataagatac atccggcaca attccgcagg
     2461 gagcgtctgg aaaccgaaga agtaacagat ctgctatgcg aaattgtgga ggcacacccc
     2521 gacaaaaagt ggatatttac cgtaagtccg ataaggcact tgaaggatac cgcacatggc
     2581 aatcaactca gtaaatccat tttgctgctg gccatagacc gacttcagca gctccatccg
     2641 caggtggaat atttcccggc atacgagatt atgatggatg aattgcgtga ttaccgcttt
     2701 tatgcggaaa atatgacgca tcctaccaac cagagcatcc gctacatctt tgatcggttc
     2761 tgcgattatg caatagatga ttcggaaatg gccacgattg ccgaagcgca gaagcgtctg
     2821 aaggcatcgc ggcacatcag ttttacttcg aaataatctt taccgagccc gtatggcccc
     2881 tatattcatt tgcatagagc gactcaatct ctacgagtga ggttgagaaa gtaccctcct
     2941 gtgtgatgta gaaacgttct tcgatggtca gatttccttc gggcaacagc tcaaaatagt
     3001 aatttgtgag tgactcatgc tgctccttgt aaaattctcc ccaaaatcct gtaaagaaga
     3061 atgatctctc atccaaagga tagaaacagg cgggacgcat cgctttcatc ctgacaaaac
     3121 tgcggttttc gctattcctg atataataac gggcaataat ctcatctcca acattgagtt
     3181 tgtcgccatc cgttatcact ttcgaatcgg aggccctaac aaattccctg ttaacctcga
     3241 tctcatttgc cgcaggcttg acatcatcga ttattgtgtc ataagtgtaa taaaccgcac
     3301 cgagctttag atcttttgca ccgcttgaaa ccagcgcatg cacggcgtca gtggtagcga
     3361 ccgtgttttc ccaggcctgg ttgtgcttct gcagaagcat ccacaatgcc agcgaatcca
     3421 ccatctcttt ctcgccgagt ctggcgaata gggtcatcag ttttgcgtga gcgtaaattt
     3481 cgctattcat aagacctctg aaaggcatta ccgcattggg gaaatagcat cctattgtag
     3541 gatttatcac cgcataatcc ttaagcgaag cattgaggtc ggcaattcgg gagtcaaatt
     3601 ttttgctcca aaaaccggtc tccttgcaat tgagcagtat atgggcgagt tgtgctttat
     3661 tcaaaatggc aatcttctgc cacccatcag cagtatcgga gaggaatttg ctgaaggcag
     3721 tcgaaacctt ccccttcatc tgatactcca aatagtgact gcgcacatag aagagattga
     3781 ttgagttgaa ggaattatat ctctcgcgac tgtaggcttc acggatcctc tcatctatgt
     3841 aacccaccgc tccttccaca agttttttct ccgcctcact aaactctatc gcactgaaat
     3901 cgcgcaactg ggccatcttt tcgaggaaaa acaatgtgag ccgttcggaa gagggcattc
     3961 cgtcaaacca ggcgaaaccg ccgtctatat tgtggagttt ggaaagcgct tttatcgcct
     4021 cttcccggaa actctcaacc aatgcaccat ccacttcctt ctccctacca tccatggtca
     4081 gcacttttgc cctcatctga ttgatgtaaa gtgcgtttgt ccactctatg gcattgtttg
     4141 attcgggctt tgaaaccacc ggcagggcct cttttacggc agtaagtgtg gagtattcga
     4201 cgtgtcttat tcttggattg cgtgccttga tgcgtttgtg aagctgatcc tcataatatt
     4261 tgtggccgtg agagccgcct atgacaaaag aggccgcttc ggtaatggta atctccttcg
     4321 gaagaatggt gacaatatgt ttttcaccat cactataatt gcccgcttta aagctgatgg
     4381 caactcctag tttactcaca ttttcgggtg gagttacgct ccaggcgagc tccttctgac
     4441 tgccgactgc aagagtcagg gccttcacat ccgtatcttt aaggattaac tcctcccctg
     4501 tctcttcgtt gtaaaacttt atgtaaccat tgcccttaag ttgttcttcg cttagattga
     4561 ccaccttact ctttatgacg attttgtcgc cttcgcgaat aaagagagga atattgggta
     4621 caaccatcag ctctttgcgc actataagtg agcgctccgc attaccggtt ttaagagact
     4681 ggtcatagcc catcaccagc acacggaagg tcgagagaag gtcccctgtg gtgtactcta
     4741 cctccacttt tccatctcta ccgctcctca gatgagggta aaatgcgata agttcgccga
     4801 attgcgacct tatggtcatc acttcacttc cggacgcttc tttttcctca acgctactct
     4861 cttcttcgac attcgacacc agcggcatgg actcgactat ataattagtc atatccctgg
     4921 cagaactcct ggcaaccatg tcctttgtat cagaccgata tccgtaaagg ggctggtagc
     4981 ggtaagcaag ggttgttttt atatagggtg cggcattatg gacctttaca gatagcgggc
     5041 tgaatgagaa tgagttggct ccatacctgt cggtggtttt gtcaaagatt gagaccacat
     5101 attcggcatc gggagctccc gtaatggtga atttctcctt tgtttgaggg ccggtgcaat
     5161 ctctgaagga
//
LOCUS       DAOSIE010000089         5164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_14378_length_5164_cov_4.201214, whole genome shotgun sequence.
ACCESSION   DAOSIE010000089 DAOSIE010000000
VERSION     DAOSIE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14378_length_5164_cov_4.201214"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLI69_03755"
     CDS             complement(<1..234)
                     /locus_tag="PLI69_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HOG41636.1"
                     /translation="MVTFGEYKCNISAMFNLANFTPWVLFTDLGIILGLLLIGKIIRV
                     KVKLVQKLFIPPSLLAGLLGLIFGPNGFGWLPLS"
     gene            261..959
                     /locus_tag="PLI69_03760"
     CDS             261..959
                     /locus_tag="PLI69_03760"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOG41637.1"
                     /translation="MKKVLNIPFAALIILLLSLLTPSEVRAQRGHVMQYVIDGKDTIY
                     VDEIMPAVIHPRQTMNKREWTQYYKRVYNFSRAYPYALLVSKVIRETDSLFVTDNMSK
                     RKQDKYLNKMKDDLIKEFEPIFKNLTLRQGLMMIRLIDREVGMTPYYIIRHFFSGVTA
                     GFWQGIARMLKGDLKRSYDRFGEDKDLEELVEYWKRGEFEELYFDIFGQAPKPIYVPE
                     RFREDAPSAQSPKR"
     gene            complement(866..1621)
                     /locus_tag="PLI69_03765"
     CDS             complement(866..1621)
                     /locus_tag="PLI69_03765"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOG41638.1"
                     /translation="MGEPLHIFLSDVHLGVNDDSKGRREAFFLDYLRNLPEETAGLYL
                     LGDIFDFWVEYRDVVPRSSIRVLGELAALSDSGVKIYYFSGNHDYWLTDYLRDEIGME
                     VIKEPLIVREIAGRRVCMGHGDGLGCRNHFIRLLFKAIRNRWLIRILKFFHPWLIFRW
                     AINWSSSSRNQSNTSKYLFKGKDSDLYKYLCKYGEGKAIDCYILGHVHTEMEMEVDSG
                     GTLYLLGDWAEGASSLNLSGTYIGFGACPNISK"
     gene            complement(1622..2428)
                     /locus_tag="PLI69_03770"
     CDS             complement(1622..2428)
                     /locus_tag="PLI69_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOG41639.1"
                     /translation="MSWFEAIILGLIQGLTEFLPVSSSGHLIIAKELFGIETHDLAFE
                     VVVHAATVLSTIVVFRKSIWELLRGLFEFKYNEPTRYILLILVSMVPVMIVGFFFKDY
                     VEKLFGSGLLVVGLALMGTSFLLTLSERIKPRKSSDSEQSHSIGWKAAIWMGVAQAVA
                     VIPGLSRSGSTIATGLICGVSSEQVTRFSFLMVLIPVLGEAFLEFVGGGFAASSTAGE
                     LELLLGFAAAFLSGIFACKVMIAIVKKARLKWFALYCAIAGIACIVANMF"
     gene            complement(2425..2670)
                     /locus_tag="PLI69_03775"
     CDS             complement(2425..2670)
                     /locus_tag="PLI69_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOG41640.1"
                     /translation="MSNFSLSPKNVRFVLIGLIVMVAGYILMAGGGSSDPDVFNPEIF
                     NFRRLVISPLLVILGIVIVIVAIMKKPREDKEDKNGQ"
     gene            complement(2687..3565)
                     /locus_tag="PLI69_03780"
     CDS             complement(2687..3565)
                     /locus_tag="PLI69_03780"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOG41641.1"
                     /translation="MVAREKNIIGKRLIHSYLSSVISITLVLVLVGASALFAANAGEI
                     SRFFKENMVISLILGQSVTEEEGQQLTKRLNSYPQVLSADFISRESGTQEMKVLLGED
                     FLSVFESNPVPLSIDLHLNGDMVSRDSLVFLKSRFLSEPAVEEVVYQESLVEILNANL
                     RKIVTLLTGVVMVLLFISFVLINNTVRLNVYSRRFTIHTMRLVGATRAFIRRPFMRHA
                     LVQGAVSGLLAAGCIAGVLWWAGSSSEILLQILDKRITGFVLLGVFLLGIIICQISAF
                     FVVNKLVAISKDDLYY"
     gene            3647..4489
                     /locus_tag="PLI69_03785"
     CDS             3647..4489
                     /locus_tag="PLI69_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOG41642.1"
                     /translation="MRSSKKVLFVNSEIFPYMPESKISIVGRYLPQGTQESGKQIRSF
                     MPRYGCINERRNQLHEVIRLSGMNIVVNNIDRPLVIKVSSISSARMQVYFIDNEDYFH
                     RKQIWKDDNGNYFNDNDERGIFFARGVLETVKKLRWKPDIVHCQGWVSHFLPVYLKKV
                     YKNDPIFAEAKIVLSLYSEELDMRFSDNIVSKYLFGSLKAEDLDLLTEPTGTNLAKLA
                     IKYSDGIILGTDSLHPELESYIGSRKGMPVLQPMAIGAEETEYITEYNRFFDSFITSA
                     PTNE"
     gene            4482..>5164
                     /locus_tag="PLI69_03790"
     CDS             4482..>5164
                     /locus_tag="PLI69_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41643.1"
                     /translation="MNRFKGILSSLILSATILTSCILTDNTLGSVYIPGNQDISIKMA
                     DFDLPVTLKMADSLQTAVSASFIIGSITTNDYGTFRVGTATSITPLTDTILWGEDPVF
                     IDMYLQIAISEKRFLSPEQEFIPQNIYAYELKAPLDTTFIYNNSITEFSYLPVPVTSR
                     NSIYMGGDSLKMHFTKEYGERFFSATIEECDSTELFINKFYGLYFRTDDIEEGLTGGR
                     INYFNLGAS"
BASE COUNT         1579 a         1257 c         1047 g         1281 t
ORIGIN      
        1 agagagtggc aaccaaccaa aaccatttgg accgaatatc aggccaagaa gtcccgccaa
       61 aagactggga ggtatgaaaa gtttctgcac caacttgact ttgactctta taatttttcc
      121 tatcagaaga aggcccagga ttatacctag atcggtaaat agtacccacg gagtgaaatt
      181 ggctaaattg aacatggcac ttatattgca tttatattcg ccaaaggtaa ccattatttt
      241 gtttttacta catttgcggt gtgaaaaaag tcctaaatat accctttgca gcactcatta
      301 tactgttact ctcgctcctg acaccgtcgg aggtcagagc ccagagaggg catgtgatgc
      361 agtatgtcat tgatggaaag gacaccatat atgtagatga gataatgccg gcagtcatcc
      421 acccgcgtca aacaatgaat aaacgtgagt ggacgcaata ttataaaaga gtttacaatt
      481 tcagcagagc ctacccctac gcactgcttg taagcaaggt cataagagag acggactctc
      541 ttttcgtcac cgataatatg tccaaacgca agcaggacaa atatctcaac aagatgaaag
      601 atgacctgat caaggagttc gagcccattt tcaaaaatct taccctaaga cagggactga
      661 tgatgattcg ccttatcgac cgagaggtag gaatgactcc ctattatata atacgacact
      721 ttttttccgg tgtgaccgca ggattctggc aaggtatagc gaggatgctc aaaggtgatc
      781 taaaaaggtc ctacgaccgt ttcggtgagg acaaggatct ggaagaactt gtcgaatact
      841 ggaaaagggg tgaatttgaa gaactctact tcgatatatt cgggcaggcg ccaaagccta
      901 tatatgtacc tgaaagattc agggaagatg ctccttcagc ccaatctccc aaaaggtaga
      961 gagtccctcc actatctact tccatttcca tctctgtgtg tacgtgaccg aggatatagc
     1021 aatctatcgc cttcccctcg ccatatttac aaagatattt gtagagatcc gaatctttgc
     1081 ctttaaagag atatttgctc gtattgcttt ggttgcggct cgatgaagac caattgatgg
     1141 cccagcggaa tatgagccac ggatggaaaa atttgagtat acggataagc catctatttc
     1201 ttatagcctt aaagagtagt ctgataaaat gattgcggca tccgagtcca tctccgtgtc
     1261 ccatacagac gcgcctcccc gcaatctccc ttacaattag tggttccttt ataacctcca
     1321 tcccgatctc atcccgcagg taatctgtaa gccagtagtc gtgattgccg ctgaaataat
     1381 aaattttgac accactatca ctcaatgccg ccaattcacc cagcacacgg atggaggagc
     1441 ggggtaccac atctctatat tctacccaga aatcaaatat atcacccaaa agatagagtc
     1501 cggctgtctc ctcgggcaaa tttctcaaat aatcaagaaa aaatgcttcg cgacgcccct
     1561 tagagtcatc attgacccca aggtgcacat cggacaaaaa aatatgaagt ggctccccca
     1621 tctaaaacat attagccacg atacaggcaa tacccgcaat agcacagtaa agcgcaaacc
     1681 acttgagacg tgccttcttc acaattgcta tcatcacctt gcatgcaaat atacctgaaa
     1741 ggaaagctgc tgcaaaaccc aacagcaact ctagttcgcc cgcagtggaa gatgcggcga
     1801 atccacctcc cacaaactcg agaaaggcct ctcccaacac gggaatgagt accatcagaa
     1861 acgaaaacct tgtcacctgc tcactgctga caccacatat aagacccgtt gcgatagtcg
     1921 agccggagcg cgaaagaccc ggtatgactg caactgcctg cgccacaccc atccaaatgg
     1981 cggccttcca tccgattgag tggctctgct ccgagtcgct acttttacgg ggcttaatac
     2041 gctcgctcag agtaagcaaa aaggaagtac ccatcagagc cagacccaca accagcagac
     2101 cggacccaaa gagtttctca acataatcct tgaagaaaaa cccgacaatc attacgggaa
     2161 ccatcgaaac cagtatcaac aaaatatacc tggtaggctc attatattta aactcgaata
     2221 aaccacggag caattcccag atgcttttgc ggaatacgac tatagtgctc aatacagtag
     2281 ctgcatgcac caccacctcg aatgccagat catgtgtctc aatgccgaac aactccttgg
     2341 caatgataag atgcccactg ctacttaccg gcagaaactc ggtcagaccc tgaataagac
     2401 caagaattat cgcttcaaac caactcattg tccgttttta tcctccttat cctcacgggg
     2461 ctttttcata atagccacga tgactatcac gataccgagg atcactagaa gcggagaaat
     2521 cactaaacgc ctgaaattga aaatctccgg gttgaaaacg tcagggtcgg aagagccgcc
     2581 tccagccatc aaaatgtagc ccgctaccat cactataagt cctatcagaa caaaacggac
     2641 atttttaggg gaaagtgaaa aattgctcat taatctcttc tatattttaa taatacaaat
     2701 catctttgga aatagccacc agtttgttca ccacaaaaaa ggcagaaatc tgacatataa
     2761 tgattcccag caggaaaact cccagcaaga cgaatccggt aattctctta tcaagaatct
     2821 gcagcaggat ttcactggat gaacctgccc accatagtac tccggcaatg cacccagccg
     2881 ccagaaggcc tgaaacggct ccctgtacca gtgcgtgtcg cataaaggga cggcgtataa
     2941 aagcacgtgt ggcacccacc aaacgcatcg tatgaattgt gaagcgacgc gaataaacat
     3001 tgaggcgcac cgtgttattg atcaatacga aggagatgaa gagaagcacc attacaacgc
     3061 ccgtaagcag ggtcacgatc tttcgcaggt tggcattgag tatctccaca agcgactcct
     3121 gatataccac ctcttctact gccggttcgg agagaaagcg actcttgagg aatacaagcg
     3181 aatcgcgaga gaccatgtcc ccattcaagt gcagatctat ggacaagggt accgggttac
     3241 tttcaaaaac acttaggaag tcctctccca ggagaacttt catctcttgc gttccacttt
     3301 ctctcgaaat aaagtctgct gaaagcacct gtggatagga attgagtctc tttgtaagct
     3361 gctgtccctc ctcttcggta accgactggc cgagtatcag agagatcacc atgttttctt
     3421 tgaaaaatcg tgaaatctct cccgcatttg ctgcgaatag agccgatgca cccaccagca
     3481 caaggaccag cgtaatactg atgacagagg ataaatagga gtgtatcagc cttttgccaa
     3541 ttatgttttt ttctctggct accataagtc cgtttaacaa gcatcaaagg taatcaaaaa
     3601 aaaattatta tctttgtagt tgatatgccc ataaattaat tattatatga gaagtagtaa
     3661 aaaagtactg ttcgtcaact ctgaaatctt cccttatatg cctgaatcca agatctctat
     3721 cgtgggcagg tatcttcctc aggggactca ggagagcgga aaacagatta gatcattcat
     3781 gccgcgttat ggatgcatca acgaaaggag aaaccagctt cacgaagtca tacgcctttc
     3841 ggggatgaat atcgttgtca acaatattga caggcctctc gtcatcaagg tctcttcgat
     3901 ttcatccgcc cggatgcaag tctatttcat agacaacgaa gactatttcc atagaaagca
     3961 gatttggaag gatgataatg gcaactattt taacgacaat gacgaaaggg gcatattttt
     4021 cgccagaggt gtacttgaaa ctgtaaaaaa gctgagatgg aaaccggata tcgttcattg
     4081 ccaggggtgg gtttcccatt tcctaccggt gtatcttaag aaagtataca agaatgatcc
     4141 tatttttgcg gaggcaaaga ttgttctatc gctatacagc gaagaactcg acatgaggtt
     4201 cagcgacaat atcgtatcca aatacctctt cgggtcgcta aaagctgaag atctggattt
     4261 acttactgaa cctactggca caaatcttgc gaaacttgcc atcaagtact ccgacggcat
     4321 cattcttggt accgactctc tccacccgga attagagtca tacataggca gtcgtaaggg
     4381 aatgcccgtg cttcagccga tggccattgg tgcagaggag acggaataca tcacagaata
     4441 caacaggttt tttgatagct ttataacatc agctcccacc aatgaataga ttcaagggta
     4501 tattatcgtc acttatccta tcagcaacta tactcacatc ctgcattctt acagacaaca
     4561 cgctcggctc ggtatatatt ccgggcaatc aggatatttc cataaaaatg gccgactttg
     4621 acctgcctgt aacccttaaa atggctgaca gtctccagac tgccgtaagt gccagcttta
     4681 taataggatc catcactaca aatgattacg gcacattcag agttggtact gcaacatcca
     4741 taactccttt aactgacaca atactttggg gcgaagatcc ggtttttata gatatgtatc
     4801 tccagattgc aatttctgaa aagcgctttc tgagtcccga gcaggaattc atacctcaaa
     4861 atatctacgc atacgaactc aaagcccctc tggacactac attcatttac aacaattcca
     4921 taacggaatt tagttacctc cctgttcccg tcacttcacg caactccatc tatatgggcg
     4981 gagactctct caagatgcac tttactaaag agtatggaga gcgtttcttc agtgctacga
     5041 tcgaagagtg tgacagtacc gaactcttca taaataagtt ttatggactc tattttcgca
     5101 ctgacgacat agaagaagga ctcacaggcg gacgcataaa ctattttaac ctaggagcct
     5161 caga
//
LOCUS       DAOSIE010000090         5153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_14426_length_5153_cov_3.467242, whole genome shotgun sequence.
ACCESSION   DAOSIE010000090 DAOSIE010000000
VERSION     DAOSIE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14426_length_5153_cov_3.467242"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /gene="metK"
                     /locus_tag="PLI69_03795"
     CDS             complement(<1..1159)
                     /gene="metK"
                     /locus_tag="PLI69_03795"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOG41644.1"
                     /translation="MAYLFTSESVSEGHPDKVADQISDSILDDFLRQDPESRVACETF
                     VSTGLAVVGGEVTSKAYVDIQSIVRNTIRRIGYTKSEYRFDADSCAVISAIHEQSPDI
                     AQGVDREEQGAGDQGMMFGYACKDTEEYMPLPVTLAHILLLELAKIRRESPSPMPYLR
                     PDAKSQFTIEYDDNNIPSRIHTIVLSTQHDENVDQAKIKEDVRNILLPRLLNLLTPKN
                     KALFKDDFILHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDPSKV
                     DRSAAYAARYIAKNMVAAGVADEMMVQISYAIGIARPISISVNTYGTAKVDKSDGEIA
                     AAIDQCFDLRPAAIIEKFGLKNPIYSATAAYGHFGRDPFVKEMELIRNGKKV"
     gene            1430..4693
                     /locus_tag="PLI69_03800"
     CDS             1430..4693
                     /locus_tag="PLI69_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG41645.1"
                     /translation="MKLTIKFLLSAIVAMGFSVISFAQITTSSLGGQIIESSGVPAVG
                     IAVVATHQPSGTVYGAVTNAEGRYAIQGMRVGGPYSVEISSLGFQSVTYTDIILQLGE
                     QYALNSVIKESSELLAEALVIASPTSRFSTEKTGAATNISRQQMENLPTISRSITEVT
                     KLSPYGGNGMSFVGTDGRTANFTVDGANFNNNFGLSSSLPGGGSPISIDAIEEVQVVI
                     SPYDVRQTNFIGGGVNAITKSGTNTFRGSAYVYHRNENMRGNAVDGKEIAGARNRDRT
                     TTYGATLGGPIVKNKLFFFVNFEYQKSPTVVNRWRASVDGVADPDNYISRTTQADMKR
                     VSEFLKSKYDYDTGSWTDYPADESNMKILARLDWNISRKHKLALRYNYTLNQRWNSTN
                     ASSSDCGQRTTEGRFSQYSMAFANSMYSMDNKVNTFSLDLNSRLTDNLSNQLLVTFSK
                     LDDIRGSSSKPFPFIDILDGTGTITPYMSVGYELFTWNNGVHNTVVTAKDDMTYYAGA
                     HKITGGLSYEYQMADNSYMRNGTGYYRYHSLEDFLSGATPETVNMTYGYDGELNPAAR
                     VTFHKLAAYVQDDWDINDRFKLTAGLRLETILFDNSDIMTNKAIQDQDYNGYSIDTGK
                     WPTAKVQVSPRIGFTWDVLGDKTLKIRGGTGMFTGRLPLVFFTNMPTNSGMVQYQSRI
                     TTRYTGGVGKPDPLLENFKGDLITDMDALREKMIALGKPGTVSPEDGVLPSAIQAVDP
                     KFKMPQVWKTSLAVDYAFPTSFPMSISVEGILNKTVNGVRIQDINMKPVEGFARLNGA
                     DNRHIYPSSYTYTKTSAYLLTNTNRGYGGTASVTFNMTPIKDLHFTAAYTHTVSKELT
                     GMPGSDASSAFTYIPTAEGPNNPPLHNSQYVTPDRVFASLTYRDKCHNSFSLFYETWR
                     GGGNYTYMYAKDLNNDNYNYDVIYIPKDESEIKFATQKDADRYWGFANKDPYLTKNKG
                     KYAEAYSVYSPWVHRLDFRYTHDFKVKVGKSTNTLQLNFDIQNVLNLFNSSWGVSKFM
                     NPLLNSGRILDVQSIGADGVPVFTTIEAVNADTQTWTYSHSLGQCWYVQVGIKYMFN"
     gene            4714..>5153
                     /locus_tag="PLI69_03805"
     CDS             4714..>5153
                     /locus_tag="PLI69_03805"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOG41646.1"
                     /translation="MKLRYLLTAFVATLAFAVGCVDQLPGDLASLQVSSSYISIPAGG
                     GSQTITVKATEAWAFTSVPDWLTITPSSGGAGEVAVTFSAKESLDTRAAELSIKVGAN
                     EQFINVKQQVGSGELTPSTCAQIIAGPVGKTYQAEGVCTSIANTT"
BASE COUNT         1361 a         1241 c         1139 g         1412 t
ORIGIN      
        1 ttaccttctt gccattacgg ataagctcca tctcctttac gaatggatct cgtccgaagt
       61 gaccgtatgc agccgtagct gaatagatcg ggttctttag gccgaatttc tcaataatgg
      121 ctgccggtcg gagatcgaaa cattggtcga ttgcagcagc aatctcgcca tcgcttttgt
      181 ccactttggc agtgccataa gtgttgactg aaatgcttat cggtcttgca atgcctattg
      241 cgtaggagat ctgaaccatc atttcatcgg caacgcccgc tgccaccata tttttagcaa
      301 tatatcttgc agcgtatgct gctgaacgat ctaccttact ggggtcttta cccgagaagg
      361 ctccaccgcc gtgtgcccct ttacctccgt aagtgtcaac gataatcttc ctgccggtca
      421 ggccggtatc tccgtggggg cctcctatga caaatttgcc tgtgggattg acgtggagta
      481 tgaagtcatc cttgaagagt gccttgtttt ttggagtgag aagatttaag agtcgcggaa
      541 gaaggatatt tctgacatct tcctttattt tcgcctggtc gacattttcg tcgtgctgcg
      601 tggagagcac tatggtatgt atcctgctgg gtatattatt gtcgtcatac tctatggtaa
      661 actggctttt tgcgtccgga cgcagataag gcatcggtga gggagattcc ctgcggattt
      721 ttgcaagttc caataaaaga atatgggcca gggtaactgg cagaggcata tactcctctg
      781 tatccttgca tgcgtagcca aacatcatgc cctggtcgcc ggccccctgt tcttccctat
      841 ctacgccttg tgctatgtcg ggggattgtt cgtgaatagc cgatatcact gcacaggaat
      901 cggcgtcaaa tcggtattcg ctttttgtat agccaatacg tctgatggta tttctcacta
      961 tggactgaat gtccacgtag gctttggatg taacttcgcc gccaactacg gcgagtcccg
     1021 tacttacaaa tgtttcgcaa gctacgcgag actccggatc ctgacgtaga aaatcatcca
     1081 ggatggagtc ggaaatctga tcggcaacct tgtccggatg cccttcagat actgattctg
     1141 atgtaaataa atatgccatt gttttagtct taaaaataaa atgtcaaatc acgtggaaac
     1201 gaaagatttg acatagtaag gcaattttag cattttttac agtggttgca agcagtcaaa
     1261 tctttccact tccgttttaa tgaccgcaaa tgtagtaata atccttttat ccgcaaaact
     1321 tttcaacaat atagtgttga aaagtaacat aattaaataa ggtgatttag tgcaatttag
     1381 tgtaactttg caatgataga agcgcctatt tattaaccaa atcatattta tgaaactaac
     1441 aatcaaattc ctattgtccg caatcgtggc tatggggttt tctgtaattt catttgcaca
     1501 gattactaca tcatccctag gcggacagat tatcgagtca tccggagtac cggcagtcgg
     1561 tattgcggtt gttgccacgc accaaccctc cggtactgtt tacggtgcgg tcaccaatgc
     1621 tgagggtcgc tatgcgatcc agggtatgcg tgttggtggt ccctattctg ttgaaatctc
     1681 ctccctcggt ttccagagcg ttacctacac cgatataatc cttcaactcg gtgagcaata
     1741 cgccctaaac tccgtcatta aggagagttc ggaactcctt gccgaggcat tggtaattgc
     1801 ttctcccact tcaaggttct caactgagaa aacaggtgca gctaccaata tttccagaca
     1861 gcagatggag aatcttccta ctatcagccg cagtatcact gaggttacca aactttcccc
     1921 ctatggtgga aacggtatga gtttcgtggg tactgacgga cgtaccgcaa actttactgt
     1981 tgacggtgcc aacttcaaca acaacttcgg tctgagttcc agccttcccg gtggaggaag
     2041 cccgatctca atcgacgcca tcgaagaggt gcaggttgtg atctcccctt atgacgtgag
     2101 acagaccaac tttatcggtg gtggagtaaa cgccattacc aagtcgggta ccaatacctt
     2161 cagaggctcc gcctacgtat accatcgtaa tgagaatatg cgcggtaatg ccgttgacgg
     2221 taaagaaatc gcaggtgcac gcaacagaga cagaactacg acttacggtg ctaccctagg
     2281 tggtcctatc gtaaagaaca aactcttctt tttcgtcaac ttcgagtatc agaagagccc
     2341 cactgttgta aaccgttgga gagcttccgt tgatggtgtg gcagaccccg acaactacat
     2401 ctcccgtaca acccaggcgg acatgaagag ggtttcagaa tttctgaaga gcaaatatga
     2461 ttacgacaca ggttcctgga cagattatcc cgccgacgag tcaaatatga agattctcgc
     2521 tcgtttggac tggaacatca gccggaaaca caaacttgcc ctgcgttaca actacactct
     2581 caaccagaga tggaattcaa ccaacgcatc ttcatcggac tgcggacaac gtacaactga
     2641 aggacgtttc tcacagtact ccatggcttt tgccaactca atgtattcaa tggacaacaa
     2701 ggtgaacacc ttctcattgg atctcaacag ccgtctgacc gacaatctct ccaaccagct
     2761 cctagtgacc ttctccaaac tcgatgatat ccgcggatcc agttcgaaac ccttcccctt
     2821 catcgacatt cttgacggta cgggaacaat cactccttac atgtctgtag gttatgagct
     2881 ttttacctgg aacaatggtg tgcacaacac agtggtaacc gctaaggacg atatgaccta
     2941 ctacgcggga gcccacaaga tcacaggtgg tctcagttat gaatatcaga tggctgacaa
     3001 ctcctatatg cgtaatggta caggttacta tcgctaccat tcacttgagg atttcctaag
     3061 tggtgctact ccggagactg tcaacatgac ttacggttat gatggagaac tcaatcctgc
     3121 cgcccgtgtt actttccata aattagctgc ttatgtacag gatgattggg atattaatga
     3181 cagattcaaa cttacagccg gtctccgtct cgaaacaatt ctttttgaca actccgacat
     3241 aatgacaaat aaggccatcc aggaccagga ctataacggt tacagtatcg ataccggtaa
     3301 gtggcctact gccaaggtac aggtttctcc ccgcataggt tttacctggg atgtcctcgg
     3361 tgacaagact ctaaagatac gcggtggtac aggtatgttt accggccgtc tccctctggt
     3421 gttctttacc aatatgccga ccaattcagg tatggttcag taccagtcca ggataaccac
     3481 cagatatacc ggtggtgtag gtaaacccga tcctcttctc gagaatttca agggagacct
     3541 tatcaccgat atggatgcac tccgcgaaaa aatgatagca ctaggtaagc ctggtacggt
     3601 ttctcctgag gatggtgttc ttccttcagc cattcaggct gtggatccca agttcaagat
     3661 gccacaggta tggaagactt ctcttgcggt tgactatgca ttccccactt cattcccgat
     3721 gagcatttcc gttgagggta tattaaacaa gaccgtcaac ggtgttcgta tacaggatat
     3781 caatatgaag ccggtagaag gatttgcaag actcaacggt gccgataacc gacatatcta
     3841 tccttcaagt tatacttata ctaagacttc agcatatctg cttaccaata ccaacagggg
     3901 ttatggtgga acagccagtg ttacatttaa catgacgccc atcaaggatc tccatttcac
     3961 cgcagcttat acgcatacag tatctaaaga acttaccggt atgccgggta gtgatgcctc
     4021 ttctgcattt acatacatcc ctactgcaga gggtcccaat aatcctcccc ttcataattc
     4081 acagtatgtg actcctgatc gcgtattcgc ctctctcact tacagggata agtgccacaa
     4141 cagcttcagt ctcttctacg agacctggag gggcggtggc aactacacct acatgtatgc
     4201 aaaggatctc aacaatgaca actataacta cgatgttatc tacattccta aagatgagtc
     4261 ggagattaaa tttgcaactc aaaaagatgc agatcgttac tggggatttg ccaataagga
     4321 tccatacttg accaaaaata agggtaagta tgctgaggca tatagtgttt atagcccttg
     4381 ggtacacaga cttgatttcc gctacacaca tgacttcaag gtgaaagtag gcaagtctac
     4441 aaatacccta cagctcaatt ttgacattca gaatgtgctc aacttattca actcttcatg
     4501 gggagtaagt aagtttatga atcctctact caactcaggc cgcatactcg acgtacagtc
     4561 aatcggtgct gacggcgttc cggtattcac taccatagaa gctgtcaatg cagatactca
     4621 gacctggaca tattcacaca gtcttggtca gtgctggtat gtgcaggtag gtatcaaata
     4681 tatgtttaac taatgcataa cacgaaatat aatatgaaac taagatattt actcacagca
     4741 tttgtagcga ctctggcttt tgctgtcgga tgtgtggatc aattgccggg tgatttagct
     4801 tcactacagg tttcaagttc ctatatttcc attcccgccg gaggcggcag ccagaccatt
     4861 acggtcaaag ctaccgaggc atgggcattt acttcggtgc ctgattggct caccattact
     4921 ccatcatccg gtggagccgg tgaggttgca gttactttta gcgctaaaga atctctggat
     4981 acacgtgcgg ccgagctttc cataaaggtt ggtgcaaacg agcaatttat caatgtaaag
     5041 cagcaggtag gttctggtga acttactcct tccacttgtg ctcagatcat tgccggccct
     5101 gtaggcaaga cttatcaggc cgagggcgta tgtacatcca tcgcaaatac tac
//
LOCUS       DAOSIE010000091         5133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_14494_length_5133_cov_3.856636, whole genome shotgun sequence.
ACCESSION   DAOSIE010000091 DAOSIE010000000
VERSION     DAOSIE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14494_length_5133_cov_3.856636"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="PLI69_03810"
     CDS             complement(<1..1155)
                     /locus_tag="PLI69_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HOG41647.1"
                     /translation="MFRKMVNASVRLVQRYLPEPFIFALLLTFISFAVAIPVCKQTPL
                     EVLNNWGNGVWELLAFSMQMALVLVCGSTLADAPAVKRGLEKLASMPKRPVGAIALVS
                     LVSALACWINWGFGLIIGVVFAKSIARKMRGVDYRLLIASAYSGFLVWHGGISGSIPL
                     ALATPGAALSTVTMGVVTEPIAIGNTILSIYNLGICLFVIIALVVANSLMHPKGKEVV
                     EVDPGLLEEEEDESSFKPSTPAEKAENSIVISMIIALMGFAFLVMKLFVRGESLDLNS
                     VIMLFLFLGIVFHKTPIAYVKSLNKAAVGSAGILLQFPFYAGIMGIMTGTSAEGVSLA
                     GEISTLFVKISNENTFPLLTFLSAGLVNIFIPSGGGQWAVQGPIMMPAGAA"
     gene            1253..2989
                     /gene="sulP"
                     /locus_tag="PLI69_03815"
     CDS             1253..2989
                     /gene="sulP"
                     /locus_tag="PLI69_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HOG41648.1"
                     /translation="MKTRLAQLYRPKFFELFQKGKYDKHTFVQDFIAGVIVGIVALPL
                     AIAFGIASGVTPQQGLITAVVAGFLTSLLGGSHFLIGGPTGAFIVIVAGIVGEFGIQG
                     LIIATILAGIMLVFMGIFKLGTLIKFIPYPIIIGFTSGIAVTIFSTQIKDFFGMTMAE
                     VPSSFLAKWGAYFTHLGSISISETLMSLFCLAIIIFWPRVTKKVPGSLIAIIVGTFIS
                     LLLSRFAGIDFATIGSKFPELAGGIAIPEPVAPKITLDTAVKLFQPAFTIAFLCAIES
                     LLAAMVADGATGKRHNSNTELIGQGITNIITPLFGGIPATGALARTMTNINNGGKTPV
                     AGIIHSLVLLLIFLFLMPYAIYIPLSCLAAILVMVAYNMSEWRTFKYLLKGDRADVIV
                     LLITFFLTVLVDLTVAIEVGLLMAIILFVKRVSETSSITVLESDYLAATESEERAQLE
                     STEQLSVPDGVEIFEIDGPFFFGLASKMDELDRQTKQDVVQVRIIRMRKVPFIDSTGI
                     NNLRSLHKRSKKSGIQVILSGVQDNVYDSIEKFGLLHEIGRDHVFSHIIPALAKATEM
                     VMVSEQKKKHSH"
     gene            complement(3010..4296)
                     /locus_tag="PLI69_03820"
     CDS             complement(3010..4296)
                     /locus_tag="PLI69_03820"
                     /EC_number="2.5.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HOG41649.1"
                     /translation="MKNFKPTKYVLENLATGRRFEDTGWLLEDPQGKEPSLIRAIYEK
                     KQIEFGSQEAGIYRFADWLPINHTLKGSAATQTYKSEALAAELGLPNLYISFSGYWPE
                     KNAFMTTGSFKETEAYSVCGRLSHNKKGILVVASAGNTARSFAKVCSENNIPLLLSVP
                     EDNIDALWFDKPINDCVKLISSPKGSDYYDAIALADKVCTSEMFFAEGGAKNVARRDG
                     MGTTVLSAAEVIGRIPDCYFQSIGSGTGAIAAWEANLRLIEDGRFGNHKMKLFPSQNI
                     PFTPMYDAWKVRSKTLLPMDDEEARLKAIAIDAKVLSNRRPPYAVRGGLYDAMCDAGG
                     DVELADNNDIKEMQALFLRTEGIDIHPASAVATKDMINAFNAGKMPKDAITMLNITGG
                     GEERFKRDHKVVYLKPSLIALASDEGIITRVENLFK"
     gene            complement(4450..>5133)
                     /locus_tag="PLI69_03825"
     CDS             complement(4450..>5133)
                     /locus_tag="PLI69_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG41650.1"
                     /translation="LYYSGCMSSLTPMVSQSVQKVLTTAGIDFMLMDEQGTICCGRPL
                     LLSGRTEEAKALIEKNKQIIRESGAPILLVSCAICYRIFKEDYDLEGVQVMHYTEYFE
                     QLAAEGVIKLRKQELNYVYHDPCELGRGCGIYEAPRKALARVANLVPAAKERKESICC
                     GGSLGSLTLTHEERGRITENSIRNLTANSPDRIVTACPLCMKTFSRQSEIKVVDFAQV
                     IAENIVGSM"
BASE COUNT         1334 a         1226 c         1118 g         1455 t
ORIGIN      
        1 agcagctccc gcaggcatca ttataggtcc ctgaacagcc cattgacctc cgcccgatgg
       61 tatgaatata ttgaccaggc ctgcactgag gaaggtcaga aggggaaatg tattttcatt
      121 agaaatttta acgaacagag tgctaatttc tcccgcgagt gaaacaccct cagcggaggt
      181 tcctgtcatt atacccatta ttccggcata aaatgggaat tggagcagga tacctgcgct
      241 acctaccgcc gctttgttga gtgatttgac ataggcgata ggagtcttat gaaacactat
      301 tccaaggaac aagaagagca ttatcactga gtttaaatcc aatgactcgc cacgtacaaa
      361 gagtttcatt acaagaaaag caaaccccat caaagctata atcatggaga tgacaatgct
      421 attttcggcc ttttcagccg gagtcgaggg tttgaaagag ctctcatcct cctcttcctc
      481 caatagtccc gggtccactt ccaccacctc tttgcccttg ggatgcatga gggaattggc
      541 tactaccaat gcaattatta caaataggca aatccccaga ttgtatatag aaagaatggt
      601 attgccgatg gcaattggtt ccgtaaccac acccattgtg actgtggaga gcgcggcacc
      661 gggagtggca agtgcaagag gaatggagcc tgagatgcct ccgtgccata ccaaaaatcc
      721 gctataagcg gaggcaataa gcagacgata atctacgccc cgcattttac gtgcaatgga
      781 tttggcgaat accaccccga ttatgagacc gaaaccccag ttgatccagc aggccagtgc
      841 cgaaaccagg gagactaaag ctatcgctcc aacaggtctt ttaggcatgg atgccagttt
      901 ctcgagtccc cttttgacag cgggggcatc ggccaaagtg gaaccgcaga ccaataccaa
      961 agccatttgc atggaaaaag cgagtagttc ccacactcca ttgccccaat tgttcaagac
     1021 ttccaacggt gtctgtttgc ataccggtat tgcaacagca aatgaaatga atgttagcag
     1081 caaagcaaaa atgaagggct caggcaaata cctttgtact aagcgcacac tggcgttaac
     1141 catttttctg aacataatat cggcttttga gaattaattt tcaaagttac tttaaaatag
     1201 atacttttgt gctcgaaatt gaaaagataa tcgaattaat tcagtattct aaatgaaaac
     1261 cagactggca caactttata gacctaaatt tttcgaactt tttcagaaag gtaaatacga
     1321 caaacatact tttgtacagg attttattgc gggagtaata gtaggtatag tggctttacc
     1381 gttggcaata gcttttggta tcgcctcagg agttacacca caacagggtt tgattaccgc
     1441 agttgttgcc ggttttctca catctttgtt aggagggtca catttcctga tcgggggtcc
     1501 aaccggcgct tttatcgtaa tagtggccgg cattgtggga gaattcggaa tccagggtct
     1561 cataatagct accattctgg cgggaatcat gctggtattt atggggatct tcaagttggg
     1621 aactttgata aaattcatcc cttatcccat tatcataggt ttcacttcgg gcatagccgt
     1681 gactatcttt tctacccaga tcaaagattt ctttggtatg actatggcgg aagtcccttc
     1741 cagttttctt gccaaatggg gtgcttactt cacccatctg ggttcgattt ccatttccga
     1801 gactttgatg tctcttttct gccttgccat aatcattttc tggccaaggg tcactaaaaa
     1861 agtacccggc tctctgatcg ccataattgt agggacattt atttcactgc tgctttcccg
     1921 ctttgcagga atagactttg caacaatagg cagcaaattt cccgaacttg cgggaggaat
     1981 agccattccc gagcccgtag ctcccaaaat tactcttgat acagctgtca aactcttcca
     2041 gcctgcattt accattgcct ttctctgtgc aatagagtcg cttcttgcag caatggttgc
     2101 ggacggtgcc accggcaagc gtcacaattc caataccgag ctcataggac agggtataac
     2161 aaacataata actcctcttt tcggcggtat tccggcaacc ggagctcttg cacgtacaat
     2221 gactaatatc aacaacggtg gaaagacacc tgttgcaggt attattcact ccctcgtgct
     2281 tctgctgatt ttccttttcc ttatgcctta cgccatctat attccgctct cttgtcttgc
     2341 agcaatcctt gttatggtag cctacaatat gagtgaatgg aggacattca agtatttgct
     2401 caaaggagac cgagcagatg tgatagtgtt gctgattaca tttttcctga ccgtattggt
     2461 tgacttgaca gttgcaattg aggtggggct cttgatggct atcattcttt ttgttaagcg
     2521 tgtttccgag acctcctcaa tcacggttct cgagagtgac tatctggccg caaccgagag
     2581 tgaagaaagg gcacaactgg agagtaccga acaactatcc gttcctgatg gtgtggaaat
     2641 ttttgaaatc gacggacctt tctttttcgg acttgccagt aagatggacg agcttgaccg
     2701 ccaaaccaaa caagatgttg tccaagttcg tatcatacgc atgcgcaagg tgcctttcat
     2761 cgattcgacc ggaatcaata accttcgcag cctccataaa cgctcaaaaa agtctggtat
     2821 tcaggttatt ctttccggtg tccaggacaa tgtctatgac tccatagaaa agttcggctt
     2881 acttcatgaa ataggcagag atcatgtatt ctcacacatc atccctgctt tggctaaagc
     2941 cactgagatg gtgatggtga gcgaacagaa aaagaaacat tctcattgag agtctttctc
     3001 attaagaaat tacttaaaca gattttcaac tctggtgatt atcccttcgt ctgaagccag
     3061 cgcgatgagg gatggcttga ggtaaacaac cttgtggtcg cgtttgaaac gctcttctcc
     3121 tccgcccgta atatttagca tagtaatcgc atctttcggc attttacctg cattgaaggc
     3181 attgatcata tcttttgtgg ctacggcaga tgccggatga atatcgattc cctcggtacg
     3241 aaggaagagt gcctgcatct ctttgatgtc gttgttatcc gccaattcca catctcctcc
     3301 cgcatcgcac attgcatcat agaggcctcc gcgaacggca taagggggtc ttctgttgga
     3361 aagcaccttg gcatcgattg caattgcctt gagacgtgcc tcttcatcat ccattggcag
     3421 aagagtttta ctacgcactt tccaggcatc atacatcggt gtgaaaggga tattttgcga
     3481 agggaagagt ttcattttat ggttgccgaa acggccatct tctataagac gcagattagc
     3541 ttcccaggca gcaatagcgc ctgtgccgct tcctatggat tggaaatagc agtcgggaat
     3601 gcgtccgatc acttcggctg cagaaagcac tgtcgtgccc ataccatccc tgcgggctac
     3661 attttttgcc ccgccttcgg caaaaaacat ttcggatgtg cagaccttgt ctgcaagcgc
     3721 aatagcatcg taatagtcac tccctttggg tgagctgata agtttaacgc agtcgttgat
     3781 tggtttgtcg aaccacaaag catcaatatt gtcttcagga acacttagca gtagcggaat
     3841 gttgttttct gagcagactt ttgcaaatga tctggccgtg ttacctgccg aggccactac
     3901 caaaattcct ttcttattgt gagagagcct gccacatacg gaataggcct ctgtctcttt
     3961 gaatgaaccg gtggtcataa aggcattctt ctccggccag tagccggaaa aagaaatata
     4021 gagattggga agaccgagtt ccgcagcaag agcttcactt ttgtaggtct gtgtcgcagc
     4081 agagcctttg agagtgtggt tgatgggaag ccagtcagca aaacggtaga tgcccgcttc
     4141 ctgacttcca aattctatct gtttcttttc gtaaatggct ctgattagcg aaggttcttt
     4201 cccttgcggg tcttccaaaa gccatcctgt atcttcaaat ctgcggccgg tggccaaatt
     4261 ttcaagtacg tactttgtgg gtttgaaatt cttcatagcg tttttcatta ttctttatta
     4321 ttatctctta tcacttcata aattttcact gaactctcta gccttttctc aaaaaaatca
     4381 accccactaa actctaaaat ccaataaacc ctcaattcat aactcatact acccactcta
     4441 cactacatac tacatactac ccactatatt ttcggcgatt acctgggcaa aatctacaac
     4501 tttaatttcc gattgtctgc taaaggtctt catacagagc ggacatgcag tcacaattct
     4561 atcgggggag ttggcggtaa ggttgcgtat ggaattttcc gtgattcgtc ctctctcttc
     4621 atgtgtaagg gttaggctac caagagaacc gccgcaacag atgctttctt tgcgttcttt
     4681 tgcagccggc acgaggttgg cgaccctggc cagagccttg cgtggggcct cgtagatacc
     4741 gcaaccacga ccgagctcac agggatcgtg atacacatag tttagctcct gttttctcaa
     4801 ttttattact ccttcagcag ccagttgttc gaaatactcg gtatagtgca tcacctgcac
     4861 cccttccaga tcataatcct ctttaaatat tctgtagcag attgcacaag agaccagcag
     4921 tataggtgca ccgctctccc tgatgatctg cttattcttc tctatgagtg cctttgcttc
     4981 ttcggtgcgg ccggatagca gcaacggtct tccgcaacat attgtccctt gctcatccat
     5041 cagcatgaaa tctatccccg ccgttgtgag tactttctgc acggattgag agaccatcgg
     5101 tgttaaagaa ctcatacagc cggaatagta gag
//
LOCUS       DAOSIE010000092         5130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_14503_length_5130_cov_4.394680, whole genome shotgun sequence.
ACCESSION   DAOSIE010000092 DAOSIE010000000
VERSION     DAOSIE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14503_length_5130_cov_4.394680"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="PLI69_03830"
     CDS             complement(<1..144)
                     /locus_tag="PLI69_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein, HigA family"
                     /protein_id="HOG41651.1"
                     /translation="MITLKGIDPKMIANNLTPFAPVHPGSIIKGELEFRGISQRKLAV
                     QMGI"
     gene            complement(156..470)
                     /locus_tag="PLI69_03835"
     CDS             complement(156..470)
                     /locus_tag="PLI69_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG41652.1"
                     /translation="MIVVFDKKYLQELYQYGKSSEKKYRFQPPVVRKYRDCIEFLLEA
                     SDLQTLAKYNGLNLKKLTGDKKETFSIRVDKKYRIEFTVQEVHEPAVTICLITELSSH
                     YK"
     gene            complement(834..1025)
                     /gene="rpsU"
                     /locus_tag="PLI69_03840"
     CDS             complement(834..1025)
                     /gene="rpsU"
                     /locus_tag="PLI69_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOG41653.1"
                     /translation="MIIVPVKEGENIERALKKFKRKFERTGTLRELRGRKTFEKPSVR
                     KRAQKQKAIYVQKMQLLDE"
     gene            1143..3158
                     /locus_tag="PLI69_03845"
     CDS             1143..3158
                     /locus_tag="PLI69_03845"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOG41654.1"
                     /translation="MDNNELKRFRELVVQGIPSQLPPPAIYDYEISHAPRRKDILTRE
                     EKVLALKNALRYFHKQHHKELSGEFAKELESYGRIYMYRFRPEYRITARSIEDFPHKS
                     RQAAAIMLMLSNNLDYAVAQHPHELITYGGNGAVFQNWAQYLLTMQYLSQMTDEQTLV
                     LYSGHPMGLFPSHKEAPRLVVTNGMVIPNYSSQDHWEKFNAMGVSQYGQMTAGSFMYI
                     GPQGIVHGTTITLLNAARMKNRGSEEIRGKIFVSSGLGGMSGAQAKAAVIAGVVGIVA
                     EINPRAVNKRYEQGWVDEVYTDCDLLIDRALKASSAREAVSLAYQGNVVDLWERLAER
                     EIKVDLGSDQTSLHNPFAGGYYPAGLSLEESNRMMAQEPERFKEEVYKTLRRHVAAIN
                     HLTARGMYFFDYGNAFLLEASRAGADILKADGEFRYPSYVQDIMGPLFFDYGFGPYRW
                     VCTSSRPEDLAVTDTLAIEVLEEILSTAPVEIQPQLKDNIHWIREAAANNLVVGSQAR
                     ILYADAEGRTKIALAMNKAIREGRISAPIVLGRDHHDVSGTDSPFRETSNIYDGSSFT
                     ADMAIQNVIGDSFRGATWVSIHNGGGVGWGEVINGGFGMVIDGSEEADRRIKAMLHWD
                     VNNGIARRSWARNEGAIFAIKREMERTPLLKVTLPNIADDDLVESCF"
     gene            3167..4111
                     /locus_tag="PLI69_03850"
     CDS             3167..4111
                     /locus_tag="PLI69_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG41655.1"
                     /translation="MKIIDSFIEWLGVQKRYSGRTLVLYRSAVDEFYAHIFPEGKIEA
                     GEETSVLTPLNIRGFIASGLESGLSARTMNLKLAALSSYSSYLMKQGLIPSNPVKKVF
                     RPKEDKKLPEFYTEKAMANYFKSMTEESGEQEGEESFSVFRNRTILLLLYSSGIRRAE
                     LCGLKITDFDPERSVIRVLGKGDKLREIPIPYLISQELLLYLKRINREFPGNSDGYFF
                     LTDTGRPLYPAFVNNVVKKELEKVEGFTGRKSPHLLRHSLATHLLNRGADLNSIKEIL
                     GHSSLAATQIYTHNSFEQLKNAYLTAHPRAKNGGKNGN"
     gene            4101..4391
                     /locus_tag="PLI69_03855"
     CDS             4101..4391
                     /locus_tag="PLI69_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOG41656.1"
                     /translation="MEIKVQSIKFDADQKLLDFIDNKVGKIAKYYEDIVRTEVNLSLL
                     ADPQNKNVKIIVYIPGTEIIVEKNADTFEEAIVDCVGVLKGALVSAKEKRFK"
     gene            complement(4395..>5130)
                     /locus_tag="PLI69_03860"
     CDS             complement(4395..>5130)
                     /locus_tag="PLI69_03860"
                     /inference="COORDINATES: protein motif:HMM:NF021921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOG41657.1"
                     /translation="FSFFESGISRQESNEMFRRFTGPILDALHSLGVEAYLEGRNDLM
                     IDGKKFSGNAMCFSRNRIMQHGTLLFSSSIKSLAQALKERPEKFDGKAVKSHRSRVTN
                     ISEHLSDKIDVIGFMEYLAKYVGRGSAPYRWSEEDLGAINALAESRYRRDSWNFGASP
                     KYGLNKVVKFPGGLVEISFDVSSGKISAMEIRGDFFFNRPVEEFCSSMRGVEHTPEKI
                     AGRLNEVAAGEYFCGISPEELMTLFF"
BASE COUNT         1429 a         1113 c         1182 g         1406 t
ORIGIN      
        1 aattcccatt tgaacagcaa gttttcgctg gctgatgcct ctgaattcca gctcaccttt
       61 gataatgctt ccgggatgaa ctggtgcaaa aggggtaaga ttattggcaa tcattttagg
      121 atctatgcct tttagtgtaa tcataatgag taaggttatt tgtagtggct ggataattcg
      181 gtaattaaac aaatagttac agcgggttcg tgtacttctt gtacggtgaa ctcaatgcgg
      241 tattttttat caactcttat agaaaatgtt tcctttttat cgcctgtaag ttttttaaga
      301 tttagcccat tgtatttggc aagtgtctgc aaatcggatg cttctagtaa aaattcgatg
      361 cagtctctat atttacgaac aaccggaggc tgaaaacgat attttttctc actgcttttt
      421 ccgtattgat aaagctcctg caaatatttt ttatcaaaaa caacaatcat agccttccct
      481 tcttaaccct acaaatgtag ccattatttt aaattgttcg caattttgtg aatatttttt
      541 cctatttcat actaagatag acaaacccta acaatgaagc atgctgaaaa gacaattttt
      601 aataattagg atgaaagatc atagtctgac agggttgttt gccgttggtc gttagctccc
      661 gtaacgcgaa accagcatcc agcaacacca cgaaccctgc accccgaaac ttgcaccgcg
      721 taccttgcaa tccgaaccct tcaacacgta ccccgtaaaa aaagaggctc cctttttacg
      781 gggagcctcc agctatgaaa acggtttata tgatattttt gcaaatatcc gaattattcg
      841 tcgaggagtt gcatcttctg aacgtagatg gccttttgtt tctgggctct ctttcttaca
      901 gaaggttttt caaaggtctt acggccccta agctcgcgca aggttcctgt tctctcaaat
      961 ttgcgtttga attttttcaa cgctctttca atattttcac cttcttttac cggcactatt
     1021 atcattgtgc aaacacctcc tttttttgtg gccacaaata taagcaggat tttttgtttg
     1081 acaaattatt ggagaaaaaa atgtactttt gtaagtagca tactaaagat cattaataaa
     1141 atatggataa taacgaattg aaacgcttca gggagttggt cgtacaggga atccccagcc
     1201 agctaccacc accggccata tatgactacg aaataagtca tgcgccaaga cgcaaggata
     1261 ttcttaccag ggaggagaaa gtactcgccc ttaaaaatgc tctcagatat tttcataaac
     1321 agcaccacaa ggaactttct ggggaattcg caaaagaact cgagagttac ggacgtatct
     1381 atatgtaccg tttccgtcct gaataccgca ttacggcgcg atcgatagag gattttcccc
     1441 ataaatctcg tcaggcagcg gcaatcatgc tgatgctcag caataacctt gattatgcag
     1501 tagctcagca tcctcacgaa ctcataacct atggaggtaa cggagccgtt ttccaaaact
     1561 gggcgcagta tttgctcacg atgcagtatc tctctcagat gactgatgag caaacgcttg
     1621 tgctatatag tggccatccg atgggactct tcccatctca caaggaggct ccccgtcttg
     1681 tggttacaaa tggtatggta atcccaaatt actcctctca ggatcattgg gaaaagttca
     1741 atgccatggg agtttcccag tatggccaga tgactgcggg ttcattcatg tatataggcc
     1801 cgcaggggat tgtgcatggt accaccatta ccttactcaa tgctgccaga atgaaaaata
     1861 ggggcagtga agagatcaga ggtaagattt tcgtgagttc gggccttgga ggaatgtccg
     1921 gagcccaggc caaggcggct gtgattgcag gagtggtagg tatagtggct gagatcaatc
     1981 cgagggctgt gaacaaacgt tatgagcaag gttgggtgga tgaagtatat acggattgcg
     2041 atctgcttat agacagagca ctaaaagcga gttctgcgcg tgaagcggtc tccctggcgt
     2101 atcaaggaaa tgtagtggat ctgtgggagc gtctggcaga gagagagatt aaagtagatc
     2161 tgggtagtga ccagacctcg cttcacaatc cctttgcagg aggctattat cccgccggtc
     2221 tctctttgga agagtccaac aggatgatgg cacaggagcc ggaacgcttc aaggaggagg
     2281 tctacaagac tctccgaagg catgttgctg ctatcaatca tcttacagcg cgtggcatgt
     2341 atttctttga ctatggcaat gctttcctat tggaggcctc cagggcgggg gcagatattc
     2401 tgaaagctga tggcgagttc cgctatccat cctatgttca agatattatg gggccgctct
     2461 tctttgatta cggattcggc ccttaccgat gggtatgcac ctcttcccgt ccggaggacc
     2521 tggctgttac agacaccctg gcaatcgagg ttctggaaga gattctgagt acggctcctg
     2581 tcgagataca gccgcaactc aaggataata tccactggat cagagaggcc gcagcaaaca
     2641 atctggtagt aggttcgcag gcgcgaatac tctatgccga tgcagaaggt cgcaccaaga
     2701 tagccctggc tatgaataag gcaatccgcg agggaaggat aagtgctcca atagtgcttg
     2761 gacgagacca tcacgacgtt tccggaaccg attctccttt tagggagact tccaacatct
     2821 atgacggctc ctcattcact gccgatatgg cgatacaaaa tgtcataggt gactctttcc
     2881 gcggagcaac atgggtttcg atccacaatg gaggaggagt tggttggggc gaagtgatca
     2941 atggcggatt cggaatggtg atagatggaa gtgaagaggc ggatcgccgt attaaggcta
     3001 tgctccactg ggatgtcaat aatggtattg cacggcgcag ctgggcacgt aatgagggag
     3061 cgattttcgc cattaagcgt gaaatggagc gtactccatt gctaaaagtt actctaccca
     3121 atattgcaga tgatgatctt gtggagagtt gtttttgata gcctctatga agataattga
     3181 ctcattcatc gaatggcttg gagtgcagaa aaggtattcc gggcgcacac tggttcttta
     3241 caggagtgcg gtggatgaat tttatgccca tatctttccc gaaggtaaaa ttgaggcagg
     3301 agaggagacc tctgtactta ccccgctcaa tattcgcggt tttattgcca gcggacttga
     3361 aagcggactt tctgcccgta ctatgaacct caaactggcg gctctcagca gctacagctc
     3421 ctatcttatg aaacagggtt tgattccatc caatccggtt aaaaaggtat tccgccctaa
     3481 agaggataag aagctcccgg agttctatac cgagaaggct atggctaatt attttaaatc
     3541 tatgactgaa gaaagcgggg agcaggaggg cgaggaatct ttttcggttt ttcgcaatag
     3601 gaccatacta ttgctactct attccagcgg gattcggcgg gcagaactct gtggcttgaa
     3661 aatcaccgac tttgatccgg agaggtcggt aataagggtt ttgggcaaag gagataaatt
     3721 gagggaaatt ccaattccat atttgatatc tcaggaattg ttgttatatt tgaagagaat
     3781 taacagggag tttcctggca atagtgacgg ctacttcttt ttgactgata ccggacgacc
     3841 gctttatccg gcttttgtaa acaatgtggt caaaaaggaa cttgagaaag ttgaaggttt
     3901 tacagggcgc aaatcgcccc acttgctgag acattcactt gcaacacacc tgcttaaccg
     3961 cggtgccgac ctcaacagta tcaaggagat tctgggccat tcatcacttg ctgcgactca
     4021 gatttatacg cataattcat ttgaacagct aaagaatgca tacttaacag ctcatccaag
     4081 agcaaaaaac ggaggtaaaa atggaaatta aagtacaatc aattaaattc gatgcagatc
     4141 agaaactgct ggatttcatc gacaacaagg taggtaaaat cgccaagtac tacgaagata
     4201 tcgtgcgtac cgaagtcaac ctctcattgc ttgctgaccc gcaaaataaa aatgtaaaga
     4261 ttatcgttta cattccgggt acggaaatta ttgtagaaaa aaatgccgac acatttgagg
     4321 aggctatcgt agattgtgta ggagtactca aaggagctct tgtttccgct aaggagaaac
     4381 gatttaaata agatttagaa aaataatgtc atcagctctt ccgggctgat accacaaaaa
     4441 tattcaccgg ctgccacttc attgaggcgg ccggcaattt tttcaggggt atgctccact
     4501 ccacgcattg aagagcagaa ttcctccaca ggtctgttga agaagaagtc acctcttatt
     4561 tccattgctg agatttttcc ggaggagacg tcaaatgaga tctccacaag tcctccaggg
     4621 aactttacta ccttattgag accatatttg ggcgaagcac caaaattcca ggaatcccta
     4681 cggtatctag attccgcaag ggcattaatt gcgccgaggt cctcttcact ccatctgtaa
     4741 ggagcagatc ctcgccccac atattttgcc agatattcca taaatccgat cacatcaatc
     4801 ttatcactca aatgctcgga aatgttcgtc actctggagc ggtgggactt cacggccttt
     4861 ccatcgaact tttccggacg ctctttcaga gcttgcgcaa gggattttat ggaagaactg
     4921 aatagtagcg taccgtgttg cataattcga tttcttgaaa aacacatcgc attaccggaa
     4981 aacttttttc catcaatcat caggtcgttg cgtccttcga gatatgcttc cactccgagt
     5041 gaatgaagtg catctaaaat aggtccggta aacctacgga acatctcatt gctctcctga
     5101 cgggaaattc cactctcgaa aaatgagaaa
//
LOCUS       DAOSIE010000093         5063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_14763_length_5063_cov_3.334665, whole genome shotgun sequence.
ACCESSION   DAOSIE010000093 DAOSIE010000000
VERSION     DAOSIE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14763_length_5063_cov_3.334665"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /gene="pckA"
                     /locus_tag="PLI69_03865"
     CDS             <1..1161
                     /gene="pckA"
                     /locus_tag="PLI69_03865"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOG41658.1"
                     /translation="TEEELANFGEPDFVVLTASKAKVENYEELGLNSETAVVFNLTEK
                     MQVIINTWYGGEMKKGMFSYMNYLLPLQGIASMHCSANVGEDGDTAIFFGLSGTGKTT
                     LSTDPKRQLIGDDEHGWDDDGIFNFEGGCYAKVINLSEEAEPDIYNAIKRDALLENVT
                     VDKNGKIDFTDKSVTENTRVSYPIYHIDNIVKPISKAGHAKKVIFLTADAFGVFPPVS
                     KLTPEQTQYYFLSGFTAKLAGTERGITEPTLTFSACFGAAFLSLHPTKYGEELVKRMK
                     ASGATAYLVNTGWNGTGKRISIKDTRAIIDAILDGSIDKAETSFIPVFNLEIPKALPN
                     VDTNILDPRNTYPCSCDWDRKAMDLAGKFVKNFAKFTGNPAGAALVSAGPKV"
     assembly_gap    1305..1404
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1658..4945
                     /locus_tag="PLI69_03870"
     CDS             1658..4945
                     /locus_tag="PLI69_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOG41659.1"
                     /translation="MDKLLKKLLPLLALVFFSINASAQITTSSINGQILSDSGEKLIG
                     ATVIATHMPSGTNYYAVANEDGRYAIQGMRPGGPYEVKVHLLGYQTTLFQDITLQLGV
                     AFTQDAKLVISTEELEEIVIVASPASKFTTEKTGAATNISSSQIGNMPTINRSISDIA
                     RISPYANGMSFAGGDGRSTNFTVDGANFNNNFGLSPNLPGGGNPISMDAIDEIQVVVA
                     PFDVRQTNFIGGGINAITKSGTNTFKGTAYTYFRNQEMRGNKINDKDLGDRAKESQTV
                     YGFTFGGPIIKNKLFFFTNFEMDKTPGQVINYRANNGGEKAEGNVSRTLASDMKKVQD
                     FMMQKYGYDTGSYTDFPGDQSNMKLLARIDWNINDNHRLNLRYNYTKNTAWNAPNGNS
                     SDTGYRLNGTYRVGNQSMAFANNMYSMDNKVQSISADLNSKFTDNLSNQLLVTFSKID
                     DVRGSKSAPFPHIDIMAGKDATGKQIMEPYMSLGYELFTWNNGVHNNILTIKDDITWL
                     KGKHKVLAGISYEYQFADNAYMRNGTMYYRYNSIDDFLNSRTPESFAITYGWNGEPNP
                     NARVTFNQIGLYVQDEWSVSNKFKLTYGVRFDELLFDNSDIATNKAIYGLTFRDGEKI
                     DTGLWPKANLQISPRVGFIWDVKGDKSIKVRGGTGLFAGRLPLVFFTNMPTNAGLVQN
                     SVQFRTTYKNGVPTGYDSRLEKFNGGMMTNVQDAIKALGLQSELNESNHVAGSKISGV
                     DRNFKMPQIWKSSVAVDWQLPVSFPLTLTGEFMFNKTISGVTIDNINIENTTSWERFR
                     GADNRLIYPANYAINKGKNAVILANTNKGYGYIANVTLNAQPVNNLDIMVAYTMTESK
                     EVSGMPGSDPVSTWQGLITVDGPNFGTVQRSQYVVPHKLIASVNYFIPFKFFGGKGLH
                     LNLFYSGSSGSGYSYVYSNDMNGDGINRDLMYIPKDDSEINFKTDADRAAFWKFVAQD
                     KYLSTHKGEYAEAYAARTPWVHRFDLRIAEDFAFRIGNTRHNFQLVFDFMNIGNLLNS
                     KWGVYQINAISNNSAILKYEGQNDKKQPIFSMYQKDGNYLTQSYDYSYHYDQAWKFQI
                     GIRYLFN"
BASE COUNT         1434 a         1160 c         1079 g         1290 t
ORIGIN      
        1 acggaagagg agcttgcgaa tttcggagaa cccgatttcg tcgtacttac cgcctctaag
       61 gcaaaggttg agaattatga agaactcgga cttaactcgg aaaccgcagt tgtattcaac
      121 ctgaccgaga agatgcaggt gataataaat acctggtatg gtggagaaat gaaaaagggt
      181 atgttctcct atatgaatta tctcctcccc ctccagggta ttgcctcaat gcattgctct
      241 gccaacgttg gtgaagatgg tgatacagct atcttcttcg gtctctccgg tacgggtaag
      301 actactcttt ccaccgatcc caagcgtcaa cttatcggtg atgatgaaca cggctgggat
      361 gacgacggaa ttttcaattt cgagggaggc tgctatgcaa aggtcatcaa cctcagtgaa
      421 gaggccgagc ccgatatcta taatgccata aaacgcgatg ctctgcttga aaacgttact
      481 gtcgacaaaa acggaaaaat agatttcacc gacaagagcg tgactgagaa tacccgcgta
      541 tcctatccca tatatcacat tgacaacatc gttaaaccga tctccaaggc cggtcatgct
      601 aaaaaggtaa ttttccttac cgctgacgca tttggggtat tccctcccgt atccaagctc
      661 actcccgagc agacacaata ttatttcctc agcggcttta ctgccaaact ggcaggcacc
      721 gaaaggggaa ttaccgaacc tactctcacc ttctctgcct gctttggagc agcattcctc
      781 tccctacacc ctactaagta tggtgaggag ctcgtaaaga ggatgaaggc cagcggagct
      841 accgcatacc tggtaaatac agggtggaat ggcaccggca aacgcatctc catcaaggat
      901 acccgcgcca tcattgacgc tatccttgac ggctcaatcg acaaggcgga gacttcattt
      961 attccggtat ttaatctgga gatacccaaa gctctcccta atgttgacac caatattctc
     1021 gatccacgca atacctaccc ctgctcttgc gattgggata gaaaggcaat ggatcttgca
     1081 ggcaaatttg taaagaattt cgccaagttt acaggtaatc ctgcgggcgc tgcgcttgta
     1141 tcggcaggac cgaaggtgta gtgtgtagtg tgtagtgtgg agtttagagt gtggagttta
     1201 gagtgtggag tttagagtgt ggagtgtgga gtgtggagtg tggagtttag agtgtggagt
     1261 ttagagtgtg gagtttagag tgtggagttt agagtgtgga gtttnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnagagtg tggagtgagt tccgagttta ggtttcgagt
     1441 tttgaatgag attttgtttg gaattaagag ttgagaaccg gtagcattat ttgtaggatt
     1501 tccgccatgg attttagcat gtaaatctct gaaatactga tacttaacat ttattctcta
     1561 caaaattaac ataattgtaa catagctact acatttgaaa tgacaatcat ttaataactt
     1621 tgccaaaata taaaacaact tttttacgta aaaaactatg gataaattac ttaagaaatt
     1681 attgccgctt ctggcactag tgtttttctc tattaatgcc agtgctcaga ttactacatc
     1741 aagtataaac ggccagatac tcagtgattc gggtgaaaag ctcatcggag ccactgtaat
     1801 agcaacacac atgccctcgg gcaccaatta ttatgcagta gccaacgaag atggccgcta
     1861 cgctatccaa ggtatgcgtc caggtggccc atacgaagtt aaagtacacc ttctcggcta
     1921 tcagactacc ctcttccagg atataacact tcagctcggt gtagcattta cacaagatgc
     1981 caaacttgtt atttccacag aagaacttga ggaaatcgtt attgtcgcat cacctgcctc
     2041 caaatttact acagagaaga caggtgcagc taccaatatc agttcatctc agataggtaa
     2101 catgcccact atcaaccgca gcatctccga tatcgctcgt atctcacctt atgcaaatgg
     2161 aatgagcttt gccggtggtg acggacgttc taccaacttc actgttgacg gtgcaaactt
     2221 caacaacaac tttggtctga gtccaaatct tcccggtggt ggcaacccca tctcaatgga
     2281 tgccatcgat gagattcagg tggttgtagc tccttttgac gtgcgtcaga ccaacttcat
     2341 cggaggaggt atcaatgcca tcaccaaatc aggtaccaac accttcaagg gtacggctta
     2401 tacctatttc cgcaaccagg aaatgagggg taataaaata aacgacaagg atctcggtga
     2461 ccgcgcaaaa gagtctcaga ccgtttacgg cttcaccttc ggcggcccca tcattaaaaa
     2521 caagctcttc ttcttcacca actttgaaat ggataaaact cccggtcagg tgattaacta
     2581 ccgcgccaac aatggaggtg agaaagctga aggtaatgta tcccgtacac ttgcttcaga
     2641 tatgaagaaa gttcaggatt tcatgatgca gaaatatgga tacgatacag gttcttatac
     2701 tgacttcccc ggagatcagt caaatatgaa gttacttgca cgcattgact ggaacatcaa
     2761 tgacaaccac agactgaacc tccgctacaa ctataccaag aatactgcct ggaatgctcc
     2821 aaacggcaac tcttccgaca cgggataccg tcttaacggc acttaccgcg tgggcaatca
     2881 gtccatggct ttcgccaaca atatgtattc aatggataac aaggtacagt caatttcagc
     2941 tgacctgaat agcaagttta ccgacaatct atccaaccag cttctcgtta ctttctcaaa
     3001 aatcgatgac gtcagaggtt ccaagtccgc acccttcccc cacattgaca ttatggccgg
     3061 caaggatgct acgggcaaac agattatgga gccttatatg tctctcggtt atgagctatt
     3121 tacctggaac aacggagtac acaacaacat tcttacaatc aaggatgata ttacctggct
     3181 caaaggaaag cacaaggtac ttgcaggtat aagctatgag tatcaatttg ctgacaacgc
     3241 atacatgcgt aacggtacta tgtactatcg ttacaatagt atcgatgatt tcctcaatag
     3301 caggactccg gagtcatttg caatcaccta cggctggaat ggcgaaccca atccgaatgc
     3361 ccgcgttaca ttcaaccaga tcggtctcta tgtacaggac gagtggagtg taagcaataa
     3421 atttaaactt acttatggtg ttcgttttga tgagctcctt tttgacaata gcgatattgc
     3481 caccaacaaa gccatctacg gtctcacctt cagggatggc gaaaagattg acacgggact
     3541 ttggcccaag gctaacctcc agatatctcc aagggtaggt tttatatggg atgtaaaggg
     3601 cgacaagtcc atcaaggtta gaggaggtac aggtctcttt gcaggtcgtc tcccgctggt
     3661 attcttcacc aatatgccta caaatgccgg tctggttcag aactcggtac aatttagaac
     3721 tacctacaaa aatggtgttc ctaccgggta tgacagtcgc cttgaaaaat tcaatggtgg
     3781 catgatgacc aatgttcagg atgctatcaa ggccctcggt ctccagtcag aactcaacga
     3841 aagcaatcac gttgcaggtt ccaagatttc aggtgttgac cgcaatttca agatgcccca
     3901 gatttggaaa tcttccgttg cagttgactg gcagcttccc gtttcattcc ccttgaccct
     3961 caccggtgaa ttcatgttca ataaaactat cagcggagtt accattgaca atatcaatat
     4021 tgagaatacc acgagctggg agcgtttccg cggtgctgac aatcgtctta tctatcctgc
     4081 aaactatgca atcaataagg gcaagaacgc tgtaattctg gccaatacaa ataagggtta
     4141 cggctacata gcaaatgtca ctctgaacgc ccagcctgta aacaacctgg atattatggt
     4201 tgcatacacg atgaccgagt ccaaagaggt atcgggaatg cccggtagcg accccgtatc
     4261 aacatggcaa ggacttatta ccgtggacgg tcccaacttc ggcactgttc agcgttcgca
     4321 atatgtagtt cctcataaat tgattgcgag tgttaattat ttcattccct tcaaattttt
     4381 cggaggaaag ggacttcatc ttaacctctt ctacagcgga tcatcaggca gtggctatag
     4441 ctatgtttac tccaatgaca tgaatggaga cggcataaac cgcgacctta tgtatatccc
     4501 taaggatgac agcgaaatca acttcaaaac cgatgctgac cgcgctgcat tctggaaatt
     4561 tgtagctcag gacaaatacc tcagcactca caagggagag tatgccgagg cttatgctgc
     4621 acgcactccc tgggttcacc gttttgatct tcgcatcgca gaagacttcg cattcagaat
     4681 cggtaacaca agacacaatt tccagcttgt atttgacttt atgaatatcg gcaacctctt
     4741 gaattcaaag tggggtgttt atcaaatcaa cgccatttcc aacaacagcg ccatcctcaa
     4801 gtatgaggga cagaacgata aaaagcagcc catattctcc atgtaccaga aggatggtaa
     4861 ctacctaacc caatcttacg actacagtta ccactatgac caggcatgga aattccagat
     4921 cggaatacgc tacctcttca actaagactt gaatcagcat aacaaataac gggtatgaca
     4981 gcagagtcat acccgttttt tatatacaaa ttattctccc gccttagggc cgaaagagca
     5041 cctgattaat ttcccggaac gaa
//
LOCUS       DAOSIE010000094         5028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_14888_length_5028_cov_3.685301, whole genome shotgun sequence.
ACCESSION   DAOSIE010000094 DAOSIE010000000
VERSION     DAOSIE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14888_length_5028_cov_3.685301"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="PLI69_03875"
     CDS             <1..1468
                     /locus_tag="PLI69_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOG41660.1"
                     /translation="LSLVLGGRSDISTLKDRTRNCVVEAIFTLEVGQEYILRRVISPG
                     GRSRIFIDDEPATLEQIKNLSESLVDIHSQHQHLLLSRTGFQTSILDHFAGLTLQVSE
                     YEAEHAEYLNVCREISDLEQKIAETERTRDYLEYQFAQLEGASLVEGELEELEAEQAQ
                     LANSEEILQSISRMESLFEGEEGSIASRLKEIESLLERLSTFINEFGPLAERISSSRI
                     ELKDIEAEVMSKGDSVITSPQRLQIVDERLSLLYDLMRKHAVGTISELIALRESLGTQ
                     LGKTMDLSLEKESAEKRRDALKSSCDARAEAIFNQRSQAAPMLSKEIEDAVRALEMPF
                     ALFSVKVEREGAVFLFSANGGEVKELSKCASGGELSRIMLVIKGLLARYVGMPTMIFD
                     EIDAGVSGSVADKMGRKIVDMGRNMQIFAITHLPQVASKGSAHYLVYKTFDEGDEPRT
                     QIKYIEGEERVKEIARMLSGSKLTEEALANARVLLRSE"
     gene            1498..2307
                     /gene="nagB"
                     /locus_tag="PLI69_03880"
     CDS             1498..2307
                     /gene="nagB"
                     /locus_tag="PLI69_03880"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOG41661.1"
                     /translation="MRLIIQESYGKMSQWAAAYITKKINDFAPTAERPFVLGLPTGST
                     PLGTYKELIHLYNTGKVSFKNVVTFNMDEYIGIPADHPESYRTFMWTNFFSHIDIPEQ
                     NVNILNGNAGDLQKECEEYEQKIVSYGGIKLFMGGIGADGHIAFNEPGSSLTSRTRVK
                     SLTTDTIIANSRFFGHDINKVPKTALTVGVGTIMDSEEVLILVNGYNKARALYHAVEG
                     SVNHIWTISVLQMHRKGIIVCDDAATVELKVGTVNYFKDIEKYNLDPASLL"
     gene            2318..4093
                     /locus_tag="PLI69_03885"
     CDS             2318..4093
                     /locus_tag="PLI69_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOG41662.1"
                     /translation="MLTRVREYLSVERADAIIVPSNDPHFGEYVQKHFCIRALLSGFD
                     GSAGTLVITLSEAALWTDSRYFIQAEEQLHGTGIHLMKMGVDGVPDIVSWLNDRIDRG
                     GAVLLDGALFSAADYASYRKSLNTRVVGIADPFDTILPEREPLYFRPVILHKESYSGL
                     SVTAKRKIIFAGDRNDLRICRILTRCDDIMWLCNIRGSDVDYNPVALSYALLFRDKIL
                     LFACKEAFEEPALEYLVSQQVEILPYNSFYDVCEKLASDDSIHFLSPASSTSMRLESI
                     FADAKHFGYETRTEGEVAYRKSIKNSVELEGFRKANLLDGIAWCRILKYIEDNIASGT
                     LNEHSIAQQLVAYHSASPDYSGESFEPIVAFNANAASAHYSAGSDEEAAVVTAQGFLL
                     IDTGAHYPFGTTDTTRTIPLSELTSTQIADYTAVLKGMIELSMATFVKGTTGYQLDIL
                     ARQPLISRGRLFFHGTGHGIGHYLCVHEGPQSIRMDRDSGVALLPGMVQSNEPALYIE
                     GEYGIRTENVLIVEHFTENEFGTFYRFETLTLVPIATSCIDREQLGDKCVEWLNNYHK
                     RVYEKIAPHLRDEEAIWLKNKCRPI"
     gene            complement(4086..>5028)
                     /locus_tag="PLI69_03890"
     CDS             complement(4086..>5028)
                     /locus_tag="PLI69_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG41663.1"
                     /translation="KGKEEGMHILLNTIDITAYSWLGEILQDRMLRESDLMVGHIDAK
                     QINAFTDYCATNKIPFVSPLDQQTDSLMLHNPYFFQMPVTTATQTANLVGRINVGLNS
                     KLTLFSDASGSEEPYRRSILAAINSAGLPYNEIRYHIVSGRTIIDSLLTIMSPDVEHN
                     IVVASEQEAFASDVIRNMGVLKFHGYNIKVYCSNKIRNFETIEGALLHQVNTHISTNY
                     FVDYKEEATKDFILAYRALFNTEPTPFAFHGYDTFLYFIRVINALGKDFPHLIHYFPM
                     HMLQNDIHFRRTETEGGFVNIESRDVEYTPDKRITVK"
BASE COUNT         1370 a         1066 c         1212 g         1380 t
ORIGIN      
        1 tttatcacta gtgctgggtg gtcgctctga catctccaca cttaaagacc ggacacgcaa
       61 ttgcgtggta gaggcaattt ttactctgga agtgggtcaa gaatatatct tgaggagggt
      121 tatctctccc ggaggtcgtt cacgcatctt tattgacgac gaaccggcca ctttggaaca
      181 gattaaaaat ctttcggaat ctcttgtgga tatccattcg cagcatcagc acttgctcct
      241 ttcccgaacc ggatttcaaa cttcaatatt ggatcacttt gccggattga ctcttcaggt
      301 aagtgagtat gaagcagaac atgccgaata tttgaatgtt tgccgtgaaa tctcggactt
      361 ggagcagaaa atagcggaaa cagagcgtac gcgtgactat ctggagtacc agtttgcaca
      421 gttggagggg gcctctcttg tcgaaggaga acttgaagaa cttgaggcag agcaggcaca
      481 gttggccaat agtgaagaga tattgcagag catttcccgc atggaatcac tattcgaagg
      541 ggaggagggt agcatagcat cccgcttaaa ggagatagaa tcgctgctgg aaagactctc
      601 tacatttatt aatgagttcg gaccgcttgc ggagcgcatc tcctcttctc gaattgaatt
      661 aaaagatata gaggccgagg ttatgagtaa gggggactcc gtaattacct ctccgcaacg
      721 tcttcagatt gtggatgagc gtctttcatt actatatgac ctcatgcgca agcatgctgt
      781 aggtactatt agcgagttga ttgcccttag ggagtcgctt ggaactcaat tggggaagac
      841 tatggaccta tcattggaaa aggagagtgc cgaaaaacgt agagatgcac ttaaaagcag
      901 ttgtgatgct cgtgcggagg ctattttcaa tcaacgttct caggcggccc cgatgctttc
      961 aaaggagata gaggatgctg tgagggcttt ggagatgcct tttgctctat tttcagtcaa
     1021 agtggagcgt gagggagcag ttttcctatt ttccgccaac ggaggtgaag taaaagagct
     1081 ctcaaagtgt gcatcggggg gtgaactttc caggataatg ctcgttatta agggactgtt
     1141 ggccaggtat gtagggatgc ccacaatgat ttttgatgaa atagatgcgg gagtctcagg
     1201 tagcgttgca gacaagatgg gacgcaagat agtggatatg ggacgaaata tgcagatatt
     1261 tgccattact cacctgccgc aggtggccag taaaggctct gcccattatt tggtctataa
     1321 gacatttgat gagggtgacg aaccccgtac ccaaataaaa tatatagagg gagaggagcg
     1381 tgtcaaagag attgcccgta tgttgagcgg atccaaactg acggaagaag cacttgccaa
     1441 tgcacgtgtc ctacttagat ctgaatgaaa taataatata taaaaagagt aacgaccatg
     1501 agactaatta tccaagaatc ctacggaaag atgtcgcagt gggcagcggc ctacattacg
     1561 aagaaaatta atgattttgc ccccacagcc gagcgtcctt tcgtgttggg actccccacc
     1621 ggatcaaccc ccctgggcac ttacaaggag ctgatccatc tctacaatac aggtaaggtg
     1681 tcatttaaga atgttgtcac attcaatatg gatgaatata tcggtatccc tgctgatcac
     1741 cccgagagct ataggacctt tatgtggacc aatttcttct cccacataga tattcccgaa
     1801 cagaatgtca atattcttaa tggaaatgcc ggagatctcc agaaagagtg tgaggagtat
     1861 gagcagaaaa ttgtctctta tgggggcatt aagcttttta tggggggtat tggtgcagac
     1921 ggccacattg ccttcaatga gccaggctca tcactaacat ctcgtacccg tgtcaagtct
     1981 cttactaccg acactattat tgccaattcg cgcttttttg gccatgatat aaataaagta
     2041 ccaaaaacag cacttaccgt gggtgtaggt accattatgg attccgaaga ggtgcttata
     2101 ttagtgaatg gctataataa agcaagggct ctgtatcatg ctgtcgaggg ctctgttaac
     2161 catatctgga ctatttccgt actccaaatg catcgtaagg gtataattgt atgtgatgac
     2221 gctgctactg tggagctcaa agtaggtact gtcaattatt tcaaggatat cgagaaatac
     2281 aacctcgatc ccgcatcact tctctaaaag ggctatcatg ctgacccgag tacgtgaata
     2341 tctttccgta gagagggcgg atgcaatcat tgtcccctct aacgatcccc attttggaga
     2401 atatgtgcag aaacatttct gcatacgtgc ccttttaagc gggtttgatg gttctgccgg
     2461 tacgcttgta ataacacttt cggaggcagc cctatggact gactcccgat attttattca
     2521 ggcagaggag cagcttcacg gcacaggcat ccaccttatg aaaatggggg ttgatggtgt
     2581 acctgatata gtgtcttggt taaatgatcg catagatagg ggaggtgccg tactgcttga
     2641 cggggcactc ttttccgccg ccgactatgc ctcataccgc aagagtctga acacgagggt
     2701 agtcgggatt gcagatccct ttgatacaat tcttccggaa cgtgaaccac tctatttcag
     2761 gccggtaatt ttacataagg aatcctattc gggactatcg gtgacggcaa aacggaaaat
     2821 aatatttgcc ggagatcgga atgacctacg catctgtcgc atactgacgc gctgcgatga
     2881 tattatgtgg ctctgtaata tccgggggag tgatgtagat tacaatccgg ttgcactctc
     2941 ttatgctctt ctttttcgtg acaagatatt gttatttgcc tgcaaagagg cgtttgaaga
     3001 gccggctttg gaatatctcg tatcccagca ggtagagatt ctaccctata attcatttta
     3061 cgatgtgtgc gaaaagttgg cctctgacga ttcaattcat ttcttatctc ctgcctcctc
     3121 cacatccatg cgtttggagt cgatttttgc cgatgctaaa catttcggct atgaaaccag
     3181 aacagagggt gaggtagcat atcgcaagag cattaaaaac agtgttgaac ttgaaggttt
     3241 ccgtaaggca aatctcctcg acggcattgc ctggtgcaga atactcaaat acatagaaga
     3301 taacatcgca agcggcacac taaacgaaca ctccatagcc caacaactcg tcgcatacca
     3361 cagtgcctct cccgattata gcggagagag tttcgagcct atagtggctt tcaatgcaaa
     3421 tgccgcttca gcgcactata gtgccggttc cgatgaagag gcggcggtag taaccgccca
     3481 gggatttctt ttgatagaca caggagcaca ctatccattc ggtaccaccg atactacacg
     3541 taccatacct ctctctgaac ttacatcgac ccaaatcgcg gactatacgg cagtactcaa
     3601 aggcatgatt gagctttcaa tggccacttt tgtcaaaggt accaccggat atcagctcga
     3661 tatcctcgct cggcagcctc ttatctccag aggaagacta tttttccatg gcaccggtca
     3721 tggaataggc cattatctct gcgtacacga gggtcctcag agcatccgta tggatcggga
     3781 tagtggggta gctttgctcc ccggaatggt gcagtcaaat gagccggcac tctatattga
     3841 aggagaatat ggcatacgaa ccgaaaatgt acttattgtt gagcatttca cggagaatga
     3901 gtttggtact ttttacagat ttgagacgtt gactctagtg ccaattgcta cttcatgtat
     3961 tgatcgtgaa cagctaggag ataaatgtgt agagtggcta aacaattacc ataagagggt
     4021 ttatgaaaaa attgcccctc atctaagaga tgaagaggcg atatggctta aaaataagtg
     4081 tcgtcctatt tgacggttat cctcttgtcg ggagtgtact ccacatccct gctttcaata
     4141 ttgacaaatc ctccttcagt ctcggttctt cggaaatgaa tgtcattttg gagcatatgc
     4201 atcgggaagt aatgaatcaa atggggaaaa tccttcccca gggcatttat taccctgatg
     4261 aaatagagga aagtatcata tccgtggaat gcaaagggag ttggctcggt attgaacagg
     4321 gcacggtagg caaggatgaa atcctttgta gcttcctctt tataatcgac aaaatagtta
     4381 gtagaaatat gtgtattgac ctgatgcagc agagctcctt caattgtctc aaaatttctg
     4441 attttgttgg agcagtagac tttgatgtta tatccgtgaa actttagaac ccccatattt
     4501 ctgatcacat cagaagcaaa tgcttcctgc tcggaagcta cgacaatatt gtgttccaca
     4561 tcaggactca ttatagttag gagtgaatct atgatagtcc taccgctaac aatatggtat
     4621 ctgatctcat tgtaggggag tccggccgag ttgattgcag ccagaatact gcgtctgtag
     4681 ggttcctcac tgcctgatgc gtcagagaat agggtaagct tcgagttgag cccaacattt
     4741 attcttccaa ccagattggc tgtctgtgta gctgttgtta caggcatttg gaagaaatat
     4801 gggttgtgaa gcataagtga gtcggtttgc tggtctaacg gggagacaaa aggtatctta
     4861 ttggtggcac agtagtcagt gaaagcattg atttgttttg catctatatg gcccaccatc
     4921 agatcagact cccttaacat cctatcctgt aatatctctc ccagccaaga gtatgcagtt
     4981 atatcaatag tgttgagtaa aatatgcatt ccctcttctt tgcccttt
//
LOCUS       DAOSIE010000095         4952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_15178_length_4952_cov_4.623239, whole genome shotgun sequence.
ACCESSION   DAOSIE010000095 DAOSIE010000000
VERSION     DAOSIE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15178_length_4952_cov_4.623239"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..3160)
                     /locus_tag="PLI69_03895"
     CDS             complement(104..3160)
                     /locus_tag="PLI69_03895"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOG41664.1"
                     /translation="MNFRKFTLYNRIISLVILLVAGFVYLSTIEPTTSFWDCGEFIAS
                     SYKLEVGHPPGNPVFQLFARFFTIFTDSMHAAAAVNAMSAICSAFTIFFLYLTIVHLG
                     IRLNEKSGRSLTIANAVAIYGAGVVGALAYCFSDTFWFSAVEAEVYAMSSLFTAVVFW
                     AILKWEEEAESKYANRWIILISFLMGLSIGVHLLNLLAIPAITFIIYYKKSTKINWKS
                     ATGVLLLSGAVILAVMIGVVQYLPRIAAFFDRIFVNGLGAPFNLGAVIFLVLLFAACF
                     LGMYWLRKREKAILHTIVLSFTTILIGYSTFAVVVIRASANTPTNEYQPDNPYTLVRY
                     LSREQYGSNPIIYGQAYTSPYDTKKVEYYTPLDGRYYKAENIEVIFPSEGKMFFPRMW
                     NATDQRYVKLYDSYSKVKNNRTLTVRGEKQVVQMPKFKDNLRFFFDYQLNYMYFRYFM
                     WNFAGRQNDFHGQEPSDLIAGNWESGIGFIDEIRLGDQSEAPEILKNNNAKNHYYFLP
                     LILGLIGFFFQLGKDQRNCWVTSLLFILTGIAIVVYLNQPPFQVRERDYAYAGSFYVF
                     TIWIGLAVLAIQSWIEKLHKKEKGGAAIAGAGIATVLGLSVAILMGCENWDDHDRSGR
                     YTANDWGYNFLAGADPNAILITYGDNDTFPLWYQQEVEEVRTDIRIVNTSLLGTDWYI
                     DQMQRRQYDSDPLKITIPRIQYLYGTNDYPYVINAIERPILASQAIDIFRNPQYKLSD
                     GKTDFLPAKQLLIPVNKENVKKYGIVAEKDYDKIVDTVVLNINADRIGKTSLIILDFL
                     STYQWDRPVYCVTTGTDLDLGLENWLQIDGMINKFVPIHTPDFRDNPQIDEDKAYKIL
                     TETYRFDSLKDTTIHVDYQNIYSFMAVQPIREMFAKVSGKLIQSGKVDQAEVLLDMAI
                     DVMPRKNFPYNISFLRSINELSLIDMMEQYLMIGKSEKAKALADQFVEETIQISKFFG
                     TPYGNSYISTKEMDSNITLLYYVVSIFDRYDQKEFADSIKRRLIEI"
     gene            3244..3591
                     /locus_tag="PLI69_03900"
     CDS             3244..3591
                     /locus_tag="PLI69_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOG41665.1"
                     /translation="MADNDWKSRLGVVFSTNPDFRYESGEDHATVETLPVEKQRLIVS
                     IDRRNRGGKQVTLIKGFMGSEEDLATLGKTLKTKCGVGGSVKEGEIIIQGDFRDRIVT
                     LLQSMGYNARRGN"
     gene            3591..4295
                     /locus_tag="PLI69_03905"
     CDS             3591..4295
                     /locus_tag="PLI69_03905"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOG41666.1"
                     /translation="MNVRDTIQGISDCWQGSRLSLVAEPHSMPIQPPESDILLSLVVA
                     SLLVLFIIFNRRVINAVFCSFSVLTNKRRIREIFNENYNNVSIRVTFLICLPLLSFIL
                     YRTGVVAYGFLITLAALAAFFLFKQISFLIMSWLWGRKEVVHTVSRLNYATVIMVTLT
                     SIPVAVMLHIIPDMGYMVVLITLGVIFIVWYLLYLIKSVEIFISSGFSHFLGFLYLCG
                     LELLPVILLADIAIRF"
     gene            4299..>4952
                     /locus_tag="PLI69_03910"
     CDS             4299..>4952
                     /locus_tag="PLI69_03910"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOG41667.1"
                     /translation="MTIENILISQPAPTNASPYTELISKYGVNVDFHPFFRVEGVTAR
                     EFRANKVNILNYTAVVFTSKIAIDAFFKICEETRVTVPETMKYFCQSELIAQYLQTYI
                     VYRKRKIFFGNGTIASMVDNIGAKHRGENFLIACTDGLRLDVKKLFTKAKLKHGSAVF
                     VKTVSSDLSSLNLEKYQMVVFYSPSDVKSLFESYPDFKQGSLLFATYGSTTAKAMKDA
                     "
BASE COUNT         1344 a         1071 c         1110 g         1427 t
ORIGIN      
        1 acttttgtca taatttttcc tgattaaagt gtaaacctat ttcaggacat gacacgcacc
       61 cctacctact acagactaca tactacccac taccaactac atactatatc tctattaacc
      121 ggcgttttat agagtcggca aactcttttt gatcatatcg gtcgaaaatg ctcacgacat
      181 agtagagcag agttatattt gaatccattt ccttggtcga gatatatgag ttgccgtaag
      241 gcgtaccgaa gaacttcgat atctgtatgg tctcttccac aaattgatcg gcgagtgctt
      301 ttgccttttc gctcttccct atcatcaggt attgttccat catatcgatt agcgaaagct
      361 cattgataga gcgtaagaat gatatgttgt aggggaaatt cttcctgggc atgacatcta
      421 tggccatatc cagcaatact tctgcctgat cgaccttgcc gctctgtatg agtttaccgg
      481 agacctttgc aaacatctct cttatcggct gcacggccat aaatgagtag atgttttgat
      541 aatccacatg tattgtggtg tctttaagcg aatcaaaccg ataagtctcg gtaagtatct
      601 tgtatgcttt gtcctcgtca atctgaggat tatcacgaaa atcgggagtg tgtatcggta
      661 caaacttgtt aatcatacca tctatctgaa gccaattttc gagacccaaa tccaggtctg
      721 tcccggtggt aacacagtaa accggcctgt cccattggta agtcgaaagg aagtcgagga
      781 ttatgagaga ggttttgcct atcctgtcgg cgttaatatt tagtacaacg gtgtctacga
      841 tcttgtcata atccttttca gctacaatcc catatttctt gacgttctct ttgttgacgg
      901 gaatcaacaa ctgtttggca ggtagaaagt cggtcttgcc gtcggagagt ttgtattgag
      961 gattgcggaa aatatctatt gcctgtgagg ccagtatcgg tctctcaatg gcgttgatta
     1021 cataagggta gtcatttgtc ccataaagat actggattct cggtatggtg attttgagag
     1081 gatcggaatc atattgtctg cgttgcatct ggtctatgta ccaatctgtg ccgagaagcg
     1141 atgtgttcac tatgcgtatg tcagtgcgta cttcttccac ctcttgctga taccagagcg
     1201 ggaaggtatc gttgtctccg taagtaatca ggatggcatt gggatcagcg cctgcaagga
     1261 aattatatcc ccagtcattt gccgtatagc gtccgctacg gtcgtgatcg tcccaatttt
     1321 cgcatcccat cagtatggct acggagaggc ctaggaccgt agctatacct gcacctgcta
     1381 tggcggcgcc gcccttctct tttttgtgga gtttttcaat ccagctctga atcgccaata
     1441 ccgcaagacc tatccagatg gtgaagacat agaaagagcc cgcataagcg tagtcacgct
     1501 cacgaacctg gaacggaggc tggttgaggt aaaccactat ggcgattccg gtgaggataa
     1561 aaagcaggga ggtcacccag cagtttcgtt gatctttgcc tagttggaag aagaacccga
     1621 taagacccag tatcagaggg aggaaatagt aatggttctt ggcgttatta ttctttagaa
     1681 tttccggagc ctcggattga tctcccagac gtatttcatc aataaatccg ataccgctct
     1741 cccaattgcc cgcaatcagg tctgaaggct cctgtccatg gaaatcattc tgcctgcctg
     1801 caaaattcca cataaagtag cggaaataca tgtaattgag ctggtaatcg aagaaaaacc
     1861 tcagattgtc cttgaatttg ggcatctgaa ctacctgttt ttctcccctt acagttaatg
     1921 tccggttatt ttttactttg gaatagctat cgtataattt tacatatctt tgatcagtag
     1981 cattccacat tctagggaaa aacattttgc cctccgaagg gaatatcact tcgatatttt
     2041 ctgctttata gtaacgacca tccaatggag tatagtattc tacctttttg gtatcgtaag
     2101 gtgacgtata agcttgtccg taaataatgg gattgctgcc gtactgttcg cggctgagat
     2161 accttaccag agtataggga ttatcgggct gatactcgtt ggtaggagta tttgcgcttg
     2221 cgcgtataac gaccacggca aaggtagaat aacctatgag gatggtagtg aaggagagga
     2281 ctattgtatg caaaatcgct ttctccctct tcctaagcca gtacattccc aagaaacatg
     2341 cagcaaagag aagcaccagg aagattactg cgcccagatt aaacggagcc cctaagccgt
     2401 ttacaaaaat tctgtcaaag aaagctgcaa tcctgggcag atactggacc acgccgatca
     2461 tcacggccaa gatgactgca cctgagagaa gcaatactcc tgtagcgctt ttccagttga
     2521 ttttcgtcga ttttttgtaa taaattatga aggttatggc aggaatcgcc agaaggttga
     2581 gcagatgcac tcctatggag agtcccatca gaaaagatat cagtatgatc catcggttgg
     2641 catatttgct ctcagcctct tcttcccact tgaggatcgc ccaaaagacg actgccgtaa
     2701 agagggaaga catagcatac acctcagcct caactgccga aaaccagaag gtatcggaga
     2761 agcagtatgc aagcgcacct accacgccgg caccgtagat agccaccgca ttggctatgg
     2821 taaggctcct gccacttttc tcattgagcc ttatgcccag atggacaatt gtcagataga
     2881 ggaaaaatat ggtgaatgcc gaacatatcg cactcatggc attgacggct gccgcagcat
     2941 gcatgctgtc ggtaaatatg gtgaaaaatc ttgcaaaaag ctggaagacc ggattccccg
     3001 gggggtgtcc gacttcaagt ttgtaagaag atgcgataaa ctcaccgcaa tcccagaaac
     3061 tggtggtagg ctctatggtt gagagataga cgaagcccgc taccaatagt ataactaacg
     3121 aaataatcct gttgtaaaga gtgaatttcc tgaaattcat aatatctttt ataatctatt
     3181 tactttttta taaacctcaa agttaaaaat aattatgact acctttgcaa aaaataggca
     3241 actatggcag acaatgactg gaaaagcaga ttgggcgtag ttttttcaac caatccggac
     3301 ttcagatatg agagcggcga agatcatgct acggtcgaga ctcttcctgt ggaaaagcaa
     3361 cggttgattg tctcaatcga tcgtcgcaat cgcggaggca aacaggttac cctcatcaaa
     3421 ggttttatgg gaagtgaaga ggatttagct actctgggaa aaactctcaa aacaaaatgc
     3481 ggtgtgggtg gctccgtcaa agagggcgag atcatcattc aaggagattt tcgcgacagg
     3541 atagtcactt tgctacagtc gatgggctat aatgcgagaa gaggcaactg atgaatgtca
     3601 gggataccat acagggtata agcgattgct ggcaggggag tcgtctctca cttgtggcag
     3661 aaccccattc gatgcccata cagcctcctg agagtgatat tctcctctcc ctggtcgttg
     3721 catcgttgct agttcttttc attattttta accgcagggt cataaatgcg gtattttgct
     3781 ccttttcggt tttaaccaac aagaggagga tccgcgagat ttttaatgaa aattataaca
     3841 acgtttcgat ccgcgtgacc tttctaatct gtttgcctct gctaagtttc atactgtaca
     3901 gaaccggggt tgtagcatac ggatttttga tcaccctggc ggctttggca gcttttttcc
     3961 tattcaagca aatatctttt ctgataatgt cgtggctatg gggtaggaaa gaggtcgttc
     4021 atacagtttc tcgactcaat tatgcgacag tgataatggt gacgctcact tcgataccgg
     4081 tagcggtaat gctgcacatc atacccgata tgggatatat ggtggtcctg ataaccttgg
     4141 gagtgatatt tatcgtatgg tatttactct acctaatcaa gtccgttgaa atatttattt
     4201 cttcaggatt ttcgcatttt ttaggctttt tgtatctttg cggtctggaa ttgctgcctg
     4261 tcatcctttt ggctgacata gcaataaggt tttaatatat gacaatcgaa aacatactaa
     4321 tctctcaacc tgctcctacg aatgcttcgc cctacacgga gcttatttcc aaatatggag
     4381 tgaatgtgga ttttcatccc tttttcagag ttgaaggcgt tactgccaga gagttcagag
     4441 ccaataaggt caacattctc aactatacgg ctgtagtctt tacctcgaag attgccattg
     4501 atgctttttt caaaatatgt gaagagacca gggttacagt cccggagact atgaaatatt
     4561 tctgtcagtc cgagttgatt gcacaatatc tccagactta tattgtttat cgaaagagaa
     4621 aaatattttt cggcaatggg acaattgctt cgatggttga caacatcgga gcaaaacaca
     4681 ggggcgagaa cttcctgatc gcctgtactg acggactccg tctcgatgtt aaaaagcttt
     4741 ttaccaaggc aaaactaaaa cacggtagcg ccgtctttgt aaagactgta agctctgact
     4801 tgagttcact gaatttggag aaataccaga tggtggtatt ctatagtcct tcggatgtca
     4861 agtcgctctt cgaaagttac cctgatttta aacaggggtc actgctcttc gccacctacg
     4921 gctctactac tgccaaagct atgaaggatg ca
//
LOCUS       DAOSIE010000096         4921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_15296_length_4921_cov_4.483148, whole genome shotgun sequence.
ACCESSION   DAOSIE010000096 DAOSIE010000000
VERSION     DAOSIE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15296_length_4921_cov_4.483148"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLI69_03915"
     CDS             complement(<1..522)
                     /locus_tag="PLI69_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOG41668.1"
                     /translation="MEYDFYIYAAALSKLFAYKPAVALQLIERVGEIGAIFKLNKKEL
                     GEVLPFDKEIVAALTAPALLDWAQKEIDWARANGVQTLYITDQRYPYRLRECYDAPIL
                     LYYKGNADLNARRVISVVGTRKASYYGKSSCRKVIEELASTGKPLVISGLAYGIDATA
                     HRAAMEDGLPTIGV"
     gene            complement(585..1634)
                     /gene="queA"
                     /locus_tag="PLI69_03920"
     CDS             complement(585..1634)
                     /gene="queA"
                     /locus_tag="PLI69_03920"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOG41669.1"
                     /translation="MKLSQFYFELPEEMIAKYPADFRDESRLMVLNKETGEIEHKIFK
                     DIIDYCNPHDLFVFNDAKVFPARLYGNKEKTGAEIEVFLLRELNKDQRLWDVLVDPAR
                     KIRIGNKLYFGENDALVAEVIDNTTSRGRTLRFLFDGTYEEFKQTLLQLGELPIPKWI
                     RTRAEECDTERFQTIYAKKEGAVACPAAGLHFSKILFKKMELKDVDTTFLTLYMSNAH
                     FHNVDVEDLSKHKMDSEKVVISEETAAKVNKAKANGNKVFAVGITTVRALETYVTTQN
                     EIKELDTWTNRFIFPPYKFSIPDAIVTNFHLPSSTMLMNAAAFGGYDNVMNAYKVAME
                     EGYRFGTYGDAMLII"
     gene            complement(1700..2539)
                     /gene="truB"
                     /locus_tag="PLI69_03925"
     CDS             complement(1700..2539)
                     /gene="truB"
                     /locus_tag="PLI69_03925"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOG41670.1"
                     /translation="MAFFILKGCNIEDESLSGFFEEHLAPSIDEYPEGIVIPIDKPYR
                     WSSADVVRRIKFQIQKHFKQKKIKVGHAGTLDPLATGLLIVCVGKATKIAEELQSHEK
                     EYVATIEFGATTPSFDLEQEIDNYYPFEHITKEKVAEALKDFIGEQLQVPPLFSAKII
                     DGLRAYEYARLGEEVKLRKSLINITEIELLSFREASVGDSKLEENEEESIRESSPGGK
                     YFYHHSSEKSDGSRPSATIRVRCSKGTYMRSLARDLGCALGSGGYLTALRRTASGFFS
                     LNN"
     gene            complement(2542..2979)
                     /gene="rpiB"
                     /locus_tag="PLI69_03930"
     CDS             complement(2542..2979)
                     /gene="rpiB"
                     /locus_tag="PLI69_03930"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOG41671.1"
                     /translation="MATIGMASDHAGFELKEFLKPYIKSMGYDIMDFGTHSTDSMDYP
                     DTAHPLAEALESGKVDWGIAMCGSGNGISMTLNKHQGVRAALCWNPELGALSRQHNDA
                     NVLALPARFISHELALEIVRSFLSAEFEGGRHRRRVEKIAVSK"
     gene            complement(2997..4547)
                     /gene="gltX"
                     /locus_tag="PLI69_03935"
     CDS             complement(2997..4547)
                     /gene="gltX"
                     /locus_tag="PLI69_03935"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOG41672.1"
                     /translation="MKLRRVRFAPSPTGPLHMGGVRTALYNYLYAKQAGGKFILRIED
                     TDSQRFVPGSEEYVIESLSWCGIMPDEGVDKDGKIVETPSAEHPFAPYRQSQRKHIYR
                     KYAEQLVAGGNAYYAFDTPQELEELRKKAEADKSSFIYNHSSRTSLKNSLTLPAEEVE
                     RRLAEESGWTIRFKIPENRVVKMNDLIRGHIEVNTSTLDDKVLWKRADELPTYHLANI
                     VDDHLMEITEVIRGEEWLPSLPLHYLLYEAFGWEESRPQFAHLSLLLKPDGKGKLSKR
                     DGDRLGFPVFPLQWVNSDGEISRGYREDGYFPEAFVNLLAFLGWNPGTEQEIFTLDEL
                     TEVFSLERVVKSGARFNPDKAKWYNQEYLRRKSTQELVKAFRAVEGERLAAFDDKYVG
                     AAVELIRERAYFVHEFRTLCDFIFEAPKEYDPAGVNKFWKEDIKEIMLQVAKFLEGLA
                     PFDKSEIEEKLQNLITTSSWGMGTVMNTLRLILVGRSSGPGVADIMAILGKKEVLQRI
                     NNAIARLG"
     gene            complement(4547..>4921)
                     /locus_tag="PLI69_03940"
     CDS             complement(4547..>4921)
                     /locus_tag="PLI69_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41673.1"
                     /translation="LMEYIKELTNPELNSKMGLVQNDDMGENIGVTIIATGFEMTQLP
                     IISEDALKNNRIEVKYDLANNKRGVPLCPDIESQITKKEMVIGIPALITDDPQTISEL
                     ESEPALTRREMMLQKQKLKQSD"
BASE COUNT         1195 a         1182 c         1082 g         1462 t
ORIGIN      
        1 aacacctatg gtaggaagtc cgtcctccat tgctgcgcga tgcgccgttg catctatacc
       61 atatgctagc ccgcttatca ccaagggttt gccggttgag gcgagttctt cgatcacctt
      121 cctgcaactg ctctttccgt agtatgaggc cttgcgcgtg cctactacag agattaccct
      181 gcgtgcgtta aggtccgcat ttcccttata atagagcagg ataggggcat cataacactc
      241 tctcagccta taagggtagc gctgatcggt tatgtagagt gtttgcactc cgttagcgcg
      301 tgcccagtct atctctttct gagcccaatc aaggagtgcc ggggcggtaa gagccgcaac
      361 tatttcctta tcaaagggga gtacctcacc gagttctttt ttgttgagtt taaatattgc
      421 gccaatctcg ccgacccttt ctatgagttg gagagcaaca gccggcttgt aggcaaaaag
      481 tttacttaat gctgctgcat aaatgtaaaa atcatactcc ataacagctt ttttgttgct
      541 aaattatgga gtatgattca atttgaggtg cttaaaagac aattttagat aatcagcatc
      601 gcatccccat aagtaccaaa gcggtacccc tcttccatcg caaccttgta ggcattcatc
      661 acattgtcat aacctccgaa tgcagcagca ttcatcagca ttgtggatga ggggagatgg
      721 aagttggtga ctatcgcgtc aggaatcgaa aatttgtagg gagggaatat gaacctgttt
      781 gtccaggtgt ccagctcttt gatctcgttt tgagtggtaa cataggtttc gagtgcgcgt
      841 acggtggtaa tacctactgc aaataccttg ttgccattag cttttgcctt attgacctta
      901 gccgctgttt cttcggagat gaccaccttc tcggagtcca ttttgtgttt cgaaagatct
      961 tctacatcca cattgtggaa atgggcgttg ctcatataga gagtaaggaa agtggtgtca
     1021 acatccttga gttccatctt tttgaaaaga atcttgctga aatggagacc ggcggcagga
     1081 caggctacag caccttcttt ctttgcgtag atggtttgga aacgctcggt atcgcactct
     1141 tcggctcttg tgcgtatcca ttttggaatg ggcaactccc caagctgcaa gagtgtctgt
     1201 ttgaactctt cgtatgtgcc gtcaaagagg aatctcaatg tacggcctct ggatgttgtg
     1261 ttgtcaatga cttcagccac aagggcatca ttttctccaa aatagagctt gttgccgatg
     1321 cgtattttcc ttgcaggatc taccagcaca tcccataaac gctggtcttt gttgagttct
     1381 ctgagaagga aaacctctat ctcggcgcct gttttttcct tgttgccgta gaggcgtgca
     1441 gggaagacct tggcgtcgtt gaacacgaaa agatcgtgag gattgcagta atcaataata
     1501 tccttgaaaa tcttgtgctc aatctctccg gtctctttgt taagtaccat aagacgtgat
     1561 tcgtcccgga aatctgccgg atatttggca atcatttctt caggaagctc aaagtaaaat
     1621 tgcgataatt tcattgttta agaaaaatgt acaaaaaaca ctctcttata cgaataaaaa
     1681 accaaaaagt ttcaaaaagt caattattta gtgaaaaaaa tcctgaggcg gtcctgcgaa
     1741 gggctgtcag atagcctcca cttccgagag cgcaaccgag gtcacgggcg agggaacgca
     1801 tataagtccc tttgctgcaa cgcactctga ttgtagcgct tggacggctc ccgtcactct
     1861 tttcacttga atgatgatag aaatatttcc cgcccggaga tgactcccgg atactttctt
     1921 cttcgttttc ttccaacttt gaatccccta cggaggcctc cctgaacgag agcagttcta
     1981 tctccgtgat gttgattagg gatttgcgta gcttcacctc ttcgcctaga cgagcatatt
     2041 cgtaagcacg caatccatcg ataatctttg cggaaaaaag aggcggcacc tgtagttgtt
     2101 ctccaataaa atcctttagc gcttcggcaa ccttctcttt ggtaatgtgt tcaaagggat
     2161 agtagttgtc aatttcctgt tcaaggtcaa aagagggggt agttgcacca aactctatag
     2221 tggcaacata ctccttttcg tgagactgaa gttcttcagc tatcttggtg gctttgccta
     2281 cgcatacgat tagcagacca gttgccaggg gatcaagtgt gccggcatgg cctactttta
     2341 tttttttctg tttgaaatgt ttctggattt ggaatttaat ccttcttacc acatcggcag
     2401 aactccacct gtagggcttg tcaataggaa ttactatgcc ttccggatat tcgtctatag
     2461 aaggagcaag gtgctcttca aaaaatccgg agagtgattc atcttctata ttgcatcctt
     2521 tcagtatgaa aaatgccata tctatttgct tactgcaatt ttctccaccc tgcgtctatg
     2581 ccggccacct tcaaattctg cggataaaaa actcctgaca atctccaggg caagctcatg
     2641 cgagataaat ctggcaggaa gcgcaaggac atttgcatca ttgtgctgcc gtgagagagc
     2701 gccgagttcg ggattccagc aaagggctgc ccttacccct tgatgcttgt taagggtcat
     2761 tgaaatgccg ttgccggagc cacacatggc tataccccaa tccacttttc cggattccag
     2821 agcttctgca aggggatgtg ccgtgtccgg atagtccatg ctatcggtag aatgggtgcc
     2881 gaaatccata atatcgtatc ccattgattt gatataaggt ttaaggaact ccttgagttc
     2941 aaaaccggcg tggtcagacg ccattccaat agttgccata ttcttatata gtatcgttaa
     3001 cccaaccggg ctattgcgtt attaatcctt tgcaaaacct cttttttacc aagaatcgcc
     3061 atgatatccg ccaccccggg gccggaactt ctacctacaa gtatgagtcg cagggtattc
     3121 atcaccgtac ccatacccca actgcttgtg gtaatgagat tctgaagttt ttcctctatt
     3181 tcgctcttgt caaagggtgc caaaccctct aaaaattttg caacttgcag cataatctct
     3241 ttgatatcct ctttccagaa cttgtttact cctgcagggt catactcctt tggtgcttca
     3301 aaaatgaaat cgcaaagagt ccggaactca tgcacaaaat aggccctttc tctaatgagt
     3361 tccaccgctg cacctacata cttgtcgtca aatgcagcca gacgctctcc ctcaaccgcc
     3421 ctgaaagcct ttacaagttc ctgagtactt tttctacgca gatattcctg attgtaccat
     3481 ttagctttat cggggttgaa tcttgcgccg gatttcacca ctctttccag tgaaaagacc
     3541 tccgtcaatt cgtccagagt aaaaatttcc tgttcagtac cgggattcca ccccagaaag
     3601 gccaggagat tgacaaatgc ctccgggaaa tagccatctt cacggtagcc acgagaaatt
     3661 tccccatcgc tgttgaccca ctgcagcggg aatacgggaa atcccaatct gtcgccatcc
     3721 cttttgctaa gttttccttt gccgtcgggc ttaagcaata gtgacaggtg cgcaaattgt
     3781 ggccggctct cttcccatcc gaaagcttcg tagagcaagt agtggagagg gagcgagggt
     3841 agccactcct caccccggat aacctctgta atctccatga gatgatcgtc aacaatgttg
     3901 gcaaggtggt aggtggggag ttcatcagct cttttccaga gtaccttgtc atccaaagtt
     3961 gaggtgttta cctcgatatg gccccttata aggtcattca tctttaccac tctgttttca
     4021 ggaattttga atcgtatggt ccatccgctc tcctcagcaa gacgacgttc tacttcttca
     4081 gcaggaaggg taagcgagtt tttaagcgat gtgcgggagc tatggttgta tatgaaactg
     4141 ctcttatctg cttcagcctt tttcctgagt tcctcaagtt cttgcggggt gtcaaatgcg
     4201 taataagcat ttccaccggc taccagttgt tctgcatatt tgcgatagat atgcttgcgc
     4261 tgggattgtc tgtaaggtgc gaaaggatgt tccgcacttg gtgtttccac tatctttcca
     4321 tctttatcca caccttcgtc aggcatgatg ccgcaccagc tgagcgattc tatcacatac
     4381 tcttcggagc ctggtacaaa cctctgggag tctgtatctt caattctcaa tatgaatttg
     4441 ccacctgcct gcttagcata cagatagttg tataaagcag tcctgacacc gcccatgtga
     4501 agcggtccgg taggactcgg cgcgaagcgt actcttctta acttcattaa tcgctctgtt
     4561 ttagtttctg tttttgcaac atcatttccc gtctggttaa agcgggctca gactcaagtt
     4621 cgctgatagt ctggggatca tccgtaatga gtgcaggtat acctattacc atctccttct
     4681 ttgtgatttg agattcaata tcggggcaca aaggtacgcc tcttttgtta tttgccaaat
     4741 catattttac ctcgattctg ttatttttga gtgcatcttc gctgatgatt ggcaattgcg
     4801 tcatctcaaa tccggtagcg atgatagtca ctccgatatt ctctcccatg tcatcattct
     4861 gcacaagccc catcttggaa ttgagttcgg gattggtgag ctccttaatg tactccataa
     4921 g
//
LOCUS       DAOSIE010000097         4808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_15739_length_4808_cov_3.462655, whole genome shotgun sequence.
ACCESSION   DAOSIE010000097 DAOSIE010000000
VERSION     DAOSIE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15739_length_4808_cov_3.462655"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="PLI69_03945"
     CDS             complement(<1..1260)
                     /locus_tag="PLI69_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HOG41674.1"
                     /translation="MRKPVIITICALLFPLLSHAQSRSAAEEFQKVTDSIQNYLSSEA
                     SILGPIAIDRAVVSKKRLDIRFKPSISEYPLRERHVRDIYSIVKLLMPSKYSAYKSSF
                     TLSAIGLPLEELVSGYFSGDSSGEVLKEYRRTVYENRRRPSSPLVTNLSKGYKATKGL
                     QESHIALWQSHGLYYEQSLKRWEWQRAKLFETVEDLYTQSYVLPFLVPMLENAGANVL
                     LPRERDHQLHEVIVDNDTPGSGYSEDGKWKASPDSGFANIKAYYLYKENPFKLGSARI
                     ADAKVVGSRRGTYSTAKWIPEIPEDGDYAVYVSYHTLKNSTEAAQYEVRHKGGSTRFL
                     VNQTMGGGTWVYLGTFPFAKGSNDSQGVFLNNNSGSNKDVITADAVKFGGGMGNIARK
                     PSDTDKEGKPVVPDFDVEAEISGYPRFT"
     gene            1319..2335
                     /locus_tag="PLI69_03950"
     CDS             1319..2335
                     /locus_tag="PLI69_03950"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HOG41675.1"
                     /translation="MRKLSLANLPTKIEKLVRWSAEKGVNIYIKRDDQTGSEWNGNKI
                     RKLEFAIHEARDRGCDLLITCGGIQSNHCRATVAAATRLGMKSAVLLRIEEEPPLEGN
                     YFLDRLMGAAVRFCTRPEYSNSRTQIMEEMAQKYHKEGYKPYIIPEGASYPVGALGYF
                     HCMKEIVAQEEQMGITFDTIVVATGSGGTHTGLTLANLLLGYGKRVVGMAVCDDNAYF
                     QNVAMQMTPGCLDYLVEQGEIGREQANEFAAKVKPSDFEYIDGYVGIGYAKSRHEELE
                     HIKRLAGLEGVVFDPVYTGKATYGLDCELGKGGAFEKSQNILFIHTGGIFGLFPVSRS
                     FPLK"
     gene            complement(2270..2686)
                     /locus_tag="PLI69_03955"
     CDS             complement(2270..2686)
                     /locus_tag="PLI69_03955"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOG41676.1"
                     /translation="MRIFPKILFFLYIATLLFLTYASFAGIDLNVTRTDILGIPIDKC
                     IHFLMFLPYPILAFFAFKRKHYWRTLFYVGLTGTLIAFLLELSQGFLTTDRITEIWDL
                     VANVSAIVIGTSALLLFKRERSGDGKESEYPSGVNK"
     gene            complement(2699..3556)
                     /locus_tag="PLI69_03960"
     CDS             complement(2699..3556)
                     /locus_tag="PLI69_03960"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG41677.1"
                     /translation="MTKKKPIVALIYDFDGTLSPGNMQEFGFIQAIGKTPEEFWQNSD
                     NISSGQEMNHILAYMKLMIDEAKNAGISLKRDSFVSFGKSIELFPGVKEWFSLINEYG
                     RTKGVVIEHYINSSGLTEMIEGTAIAKEFKKIFASSFWYDEDGVAVWPAVAVDYTGKT
                     QFLFKITKGILDISDKTRVNESQKDETKLVPFNYMIYFGDGETDIPSMKVVKMFGGNS
                     IAVYQPSRREQFRTAQKLLRQERVNFICKADYSKDSEIWKVVTTIIDKAKMEHDFTRL
                     QLKMRNRSL"
     gene            3746..>4808
                     /locus_tag="PLI69_03965"
     CDS             3746..>4808
                     /locus_tag="PLI69_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG41678.1"
                     /translation="MHWDHNVLSNIDGISYTYGQICDNIARMHILFEAVGLKKGDKVA
                     LCAKNSAQWCVAFLASTAYEAVTVPLLNDFHPESISTLVNHSESKILFTNKDIWEKLN
                     LESMPLLQVAVNVDDYTILYNKIGARLEDCFANLETLFSTKFPHFSIKDVNYPTNNLD
                     DLALINYTSGTTSDPKGVMLTYGNISSNTTFAIDSIPNSVNNKLVSMLPLAHMYGLAF
                     EFLYQIAAGCHVYFLGKVPSPKVLLSSLATIQPFMIVTVPLVVEKIFRSSVFPTIQKQ
                     PIKSLMKVPLFSRIIKNRIRSRLMAAFGGKLEHLVIGGAAINKEVEDIMKDIRMPYTV
                     GYGMTECAPLLAYENWRKFV"
BASE COUNT         1329 a         1072 c         1073 g         1334 t
ORIGIN      
        1 tgtaaagcgc ggataaccgg aaatttccgc ctctacatcg aaatccggca caacaggttt
       61 cccctctttg tcggtgtcgg atggtttgcg ggcaatgttg cccatccccc ctccaaactt
      121 cactgcatca gcagttataa catctttgtt actcccggaa ttgttgttta ggaaaacacc
      181 ctgggaatca ttagaacctt tggcaaatgg aaaagtgcct aggtagaccc aggtgccgcc
      241 tcccatagtc tggttgacta gaaagcgtgt tgaaccgcct ttatgacgta cctcatattg
      301 agctgcctcc gtgctgtttt taagcgtatg gtaggagaca tataccgcgt aatcgccatc
      361 ttccggtatt tcgggtatcc acttcgccgt gctataggta cctctccggg atcccactac
      421 ctttgcatcg gcaattcgtg cactgccgag cttgaaagga ttctccttgt agaggtaata
      481 ggctttaatg ttggcaaagc cggaatcagg ggaggctttc cacttaccat cctcagaata
      541 tccggagccg ggagtatcgt tgtccactat gacttcgtgt agttgatggt cacgctcacg
      601 gggcagaagc acgttggcac ccgcattttc gagcatcgga accaaaaagg gcagaacgta
      661 gctctgagta tagaggtctt caactgtttc aaaaagcttg gctctctgcc attcccagcg
      721 ttttagcgac tgctcatagt atagtccgtg actttgccat agggcaatat ggctctcttg
      781 cagccccttc gtagctttgt aacctttaga gaggttagtt acaagtgggg aagagggtct
      841 tcgtctattc tcataaaccg tacgccggta ctccttcagc acctcaccgg agctatctcc
      901 actaaagtat cctgagacca gctcctcaag tggcagccca atggcactta gggtgaagga
      961 agatttgtag gctgaatatt tagaaggcat cagaagtttc actatggagt aaatatccct
     1021 cacatggcgt tcccgaaggg gatactcgct tatcgacggc ttgaagcgga tatcgagcct
     1081 ctttttgctc accacagctc tgtcgatggc gatcggcccc aatatagagg cctccgatga
     1141 gagataattt tggattgaat ccgtaacttt ttggaattct tcagcagcag acctgctctg
     1201 tgcgtggcta agtaatggaa agagcagcgc acaaatagtg atgataaccg gttttctcat
     1261 ttcattgaat aattgcagca aaaatagtta tatttgtcca tacttaaaac aaaggagaat
     1321 gagaaaatta tcacttgcaa atcttcccac caaaatcgaa aagctcgttc gctggagcgc
     1381 tgaaaagggg gtaaatatct atatcaaacg agacgaccag acaggaagtg aatggaacgg
     1441 caataagata cgtaaacttg aatttgccat tcatgaggca cgggaccgcg gctgtgacct
     1501 cttaatcacc tgcggaggca tacagagcaa ccattgcagg gctaccgttg cagcggccac
     1561 cagacttggc atgaagtcgg ccgtactatt gaggattgaa gaggagccgc ctttggaagg
     1621 gaactacttt cttgataggc taatgggagc tgccgtgcgt ttctgcaccc ggcccgaata
     1681 tagcaacagc cgtacgcaga taatggagga gatggcgcag aagtatcaca aagaggggta
     1741 taagccctat ataatcccgg aaggtgcctc ttatcctgta ggagcactgg gctatttcca
     1801 ctgtatgaag gagattgtag ctcaggaaga acagatgggg ataacttttg atacaatcgt
     1861 ggtggccacc ggttccgggg ggacacacac cggacttacg ttggcaaacc tgcttctggg
     1921 atatggtaaa agggtagttg gaatggctgt ttgtgatgat aatgcctact ttcaaaatgt
     1981 agccatgcaa atgactcccg gatgtctgga ctatctggta gagcagggag agatcggcag
     2041 ggaacaagcc aatgagtttg cagcaaaggt taaaccctcc gatttcgaat atattgacgg
     2101 atatgtggga ataggatatg ccaagagccg tcacgaggag ttggaacaca taaagcgtct
     2161 tgccggactt gaaggggtcg tattcgaccc tgtatatacc ggaaaggcca cttatggtct
     2221 ggattgtgaa ttgggcaaag gcggtgcctt tgaaaagtca caaaacatcc tatttattca
     2281 caccggaggg atattcggac tctttcccgt ctccagatct ttccctctta aatagtagca
     2341 gagccgaagt tccaatcact attgcactca cattggctac cagatcccaa atttccgtta
     2401 tgcggtcggt agtcaagaat ccttgcgaca attcgagcaa aaatgcgatt agtgtacctg
     2461 tcaaaccgac atagaaaagc gttctccagt aatgctttct cttaaaagcg aaaaaagcca
     2521 gaatcggata ggggagaaac ataagaaagt gaatacattt atcaattgga attcctaaaa
     2581 tatccgtgcg tgtaacattc agatctatgc cggcaaagct cgcataagtg aggaagagta
     2641 gggtcgcgat atataggaag aaaagtattt taggaaatat tctcattagg tatatatttt
     2701 ataatgacct gttgcgcatc ttaagctgca aacgtgtgaa gtcatgttcc atttttgctt
     2761 tgtcaattat ggtggttaca actttccata tctcgctatc ctttgaatag tctgccttac
     2821 agataaagtt gacccgttcc tgccgtagaa gtttttgggc agtacggaat tgttctctcc
     2881 ttgagggctg ataaacagct atggagttgc ctccaaacat cttcacaacc ttcatgctgg
     2941 gaatatccgt ctctccgtca ccaaagtaga tcatataatt gaaagggacc agcttagttt
     3001 catctttctg actctcattt actctggtct tatcactgat atccaggatg ccttttgtga
     3061 ttttaaagag gaattgggtc tttccggtgt agtccacggc aacggccggc cataccgcta
     3121 caccatcttc atcataccag aaggaagagg cgaaaatctt cttgaattcc ttcgcaattg
     3181 cagtcccctc tatcatctcc gtcaatcctg aggagttgat atagtgttct atgacgactc
     3241 cttttgtgcg tccatattca tttattagtg aaaaccactc tttaactccc ggaaataact
     3301 caatcgactt tccaaatgag acaaaagaat ccctttttag agagataccc gcatttttag
     3361 cctcgtctat catcagtttc atgtaagcaa gaatgtggtt catttcttgc ccggatgaaa
     3421 tattatcact attttgccaa aactcttcag gtgtctttcc aatagcctga atgaaaccga
     3481 attcctgcat gttccctggc gaaagggtgc catcgaagtc ataaatgaga gcgacaatgg
     3541 gttttttctt tgtcataatt caattttttt gtcacatttt tcacaaactc acccctgcat
     3601 gatgatttta accactttat tggtgaatat atcacagcgc taattagtta tgtattatat
     3661 atgatatatt tgcacatcaa atttttgaga aatggcaagg gacaaaatta cacacttttt
     3721 aacgtatttt caaaataatg ccgcattgca ttgggatcat aatgtactga gcaatattga
     3781 cggcatttca tatacttacg gtcagatttg tgacaacatt gcgagaatgc atattctctt
     3841 cgaggcggta ggccttaaaa agggagataa agttgcactt tgtgccaaaa attccgcaca
     3901 atggtgcgta gcattcctcg cttccactgc ttatgaggca gtgaccgtgc ctttgctcaa
     3961 cgattttcat ccggagagca tttccacact agtaaaccat tcagaaagca aaatactctt
     4021 tacaaataag gatatctggg agaagctcaa tctcgagagc atgccacttt tacaggtagc
     4081 cgtaaatgta gatgattaca caatattgta taacaagatc ggagccaggt tggaggattg
     4141 tttcgctaat cttgagactc ttttttctac caagttcccc catttctcaa tcaaggatgt
     4201 aaattatcca acgaacaacc ttgacgatct ggctctgatc aattatacat ccggtaccac
     4261 cagcgatccc aaaggggtta tgctgaccta cggaaatatc agctcaaaca ccacttttgc
     4321 catcgattcg attcccaatt ccgtcaacaa caagctggtc tccatgcttc cgttggcaca
     4381 catgtatgga ctggcattcg aattcctata tcaaattgca gcaggctgcc atgtttattt
     4441 ccttggcaaa gtgccttcac cgaaagtgct tctcagctca cttgcaacga ttcagccatt
     4501 tatgattgtt acggtgccgc ttgttgtcga aaaaatattc aggagctccg tattccctac
     4561 aatacagaag cagccgataa aaagtttgat gaaagtgccg ttattctcac gcatcatcaa
     4621 aaataggatc agaagcagat tgatggctgc cttcggtgga aaactcgaac atctggtaat
     4681 cggcggtgcc gctatcaata aagaggtgga ggatattatg aaagatatcc gtatgcctta
     4741 taccgttggt tacggcatga ctgaatgtgc ccctcttctc gcttatgaga attggagaaa
     4801 attcgtta
//
LOCUS       DAOSIE010000098         4803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_15762_length_4803_cov_3.645535, whole genome shotgun sequence.
ACCESSION   DAOSIE010000098 DAOSIE010000000
VERSION     DAOSIE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15762_length_4803_cov_3.645535"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="PLI69_03970"
     CDS             complement(<1..175)
                     /locus_tag="PLI69_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOG41679.1"
                     /translation="MPTIQQLVRKGRKVIVEKSKSRALDACPQKRGVCVRVYTTTPKK
                     PNSAMRKVARVRLT"
     gene            complement(285..1949)
                     /locus_tag="PLI69_03975"
     CDS             complement(285..1949)
                     /locus_tag="PLI69_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41680.1"
                     /translation="MKRDFTIITLLTGVLLLLSAPLFAQRVERVWFSTDRNIYIAGER
                     IWCSALCMDAATRTPSELSSIAYMELHSASGVAQTAKVALMEGRGAGYIDLPNTLPTG
                     NYRLIAYTSANLNERGYDFEAAFSREISIFNVLSTARSGDVKVVGESDYIAAVAAAPS
                     IKPCADVQIIVASEAGRLNQIPVSIVNNTGEEITFSLSIFNEDGLLVGKALGPEEFLK
                     MLPDADRVIFDRNRTPEYEGEIITAQSGEKSAYISAIGNYSDIYIAETADDGTVRFFT
                     GNIYGDVELVGNISSIRQPFAEPNLNAPSTLLISESLRDRLEFRGTAMQIDRRFDSDT
                     LYELLPKRENTLLGAERITYILDDYTRFPLMEEVIVEYISQIRARKKADGTRDIQVKI
                     EDVFRVGEFAEDASLMMVDGVPIFDHEMIYRYDPMLVKSIDIYPYAYFIGNRQFDGIV
                     NMKTYKGTIPSLTLGPEVRIIEFQGAAVPMAYTCRKLAENADYPDYRQTLYWHPLLTA
                     GATGRADSSVTVICNTPDYSGTFSIVVEGITSSGNPIFVNKAFKVQ"
     gene            complement(1953..3128)
                     /locus_tag="PLI69_03980"
     CDS             complement(1953..3128)
                     /locus_tag="PLI69_03980"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOG41681.1"
                     /translation="MKERLCILILAFVAFLTGSCIYPFTPEIEDLDLGLFVIEGDIRI
                     GTESYIFVRNTQRLTATGSPVDIMADVWVENDKGEITRASNYRDGAHIIDTRNLDPRA
                     SYRLHVKPYSGMGYIASPGYNTEYVTDWLEVLVTPEIEEVSFRVGELEEKITFHVSTT
                     NPDTNPYYKWNCNEVWEYTSQFYASHVFNPNRNTVEESPNENRHYCWDRDVVPDIMIA
                     STDALSENRLVNHQIYQLPFASDKLDYLYSIEVIQSSISAEAYIYWEVMLRNTDQPGG
                     LFSPQPSELRGNIHCVGDSTKVVLGYISASKVTSRRIFVSVNDFPMQNRRENCSAFIE
                     INLAEESKMRRYYFGGYDVVFLDEMTSITSWSERRCVDCRVKGGTKDKPDYWPNNHN"
     gene            complement(3128..>4803)
                     /locus_tag="PLI69_03985"
     CDS             complement(3128..>4803)
                     /locus_tag="PLI69_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG41682.1"
                     /translation="SDVELFKSSIPAEYGGRISSVLDIRGREGNSKKISGSLGLGVLT
                     SRFHLEGPIKKEKTTFILGGRTTYSDWILKVLPKNSEYSNGTASFNDLNAQISHRFNQ
                     KNSLHFSGYFSRDKFSFTVDTTYRYHNANFSLKWRNHFSDGHSLSLSTGYDSYGYKVE
                     DTHNDWSSFSLSSNIRQGYLNLKFQSLVAAKHTFSYGFNSTMYNVEPGNRLPYGDSSA
                     IEPRRLVTERALETALFFNDTYQPTDRLSFDFGLRLSGFLSLNSRKFYGTPELRLSGK
                     YSFLPNLSLKAGFNSMAQYIHKISNTVAISPTDTWKLADADIGPQKGWQAAAGLYWTV
                     FDHRVDLSVEGYYKMMRDYPDYKSGAILVMNENLADELVSTMGRAYGVEFMVKKPGGK
                     LNGWVSYTYSRSLLREMEDRGLQTINDGEWYNAPHDKPHDLKLVGNYKFTHRYSISVN
                     LDYSTGRPVTIPVAKYLYGGGYRLYYSVRNGYRIPDYFRLDLAINIEPSHYLRQLSHL
                     SVTFGVYNVTARKNPYSVFYTRSGSGSVSGNMLCVFAVPIPYINLNLKF"
BASE COUNT         1268 a         1103 c         1096 g         1336 t
ORIGIN      
        1 tcgtgagcct tacacgggcc actttacgca tagccgaatt aggctttttg ggagttgttg
       61 tgtaaacacg tacacatact ccgcgtttct gaggacaagc atccaatgca cgagatttgc
      121 ttttctctac aataaccttt cgtcctttgc ggacaagttg ttgaattgtt ggcataaatg
      181 gttgttaatc ttttgtttaa tgtttgtaac tccgcatttt tgcgggttgc aaagatagaa
      241 caaataatct gatttacaaa caagttgtca acaaaatttg cattttactg cactttgaag
      301 gctttattaa cgaatatggg gttgccggaa gaagtaatcc cctccacaac aatggaaaat
      361 gttccactat agtcgggtgt attgcatata acggttacag aggagtcagc tctacctgtt
      421 gctcctgccg taagcaacgg atgccagtac aaggtctgtc tgtaatcggg gtagtcggca
      481 ttttcagcca gtttgcgaca agtataggcc atagggacgg ctgctccctg aaactctatg
      541 atacgcactt cggggccaag ggtcagcgaa ggaatggttc ctttataggt cttcatattt
      601 acaatgccgt caaactgtct gttgccgata aagtaggcat aggggtagat atcaattgat
      661 ttgactagca tgggatcata cctgtagatc atttcgtggt cgaaaatagg cacaccatcc
      721 accatcatca gagaagcatc ttctgcaaac tctcctacac ggaagacatc ctcaatttta
      781 acctgtatat cccttgttcc atcagctttt ttacgggctc gtatctggga aatatattct
      841 acaataactt cttccatcag agggaagcgg gtataatcat caagaatata tgttattcgc
      901 tctgcaccga ggagtgtgtt ttcccttttt ggaagcagtt catatagggt gtccgaatca
      961 aagcgtctgt caatctgcat tgcagtgcct ctgaattcga gacgatccct gagcgattcg
     1021 ctaatcagaa gtgtagaggg cgcattgaga ttcggttcgg caaagggctg cctgatggaa
     1081 ctgatattgc caaccagttc cacatctcca tagatattgc cggtaaaaaa acgaaccgta
     1141 ccgtcatcag ctgtctcagc tatgtatatg tccgaataat tgcctatcgc cgaaatatag
     1201 gcacttttct cgccactttg cgcagtaatg atctcccctt catattccgg agtgcgattt
     1261 cgatcaaaaa taactctatc agcatcaggg agcatcttca aaaattcttc gggacccaag
     1321 gcttttccta ccaggagacc atcttcgttg aaaatcgaaa gggagaaggt tatctcttcg
     1381 ccggtgttgt taactatgct gacgggaatc tgattcaaac gtcccgcttc cgaagcaact
     1441 atgatctgta cgtcggcaca gggtttaatc gaaggcgccg ctgccactgc ggctatgtaa
     1501 tcgctctctc ctaccacttt cacatccccg ctcctcgcag tcgacaacac attgaagatg
     1561 gaaatttctc tactaaaagc cgcttcgaaa tcatatcccc gctcgttaag attagccgat
     1621 gtataagcga taagacggta attgccggta ggaagagtat tgggcaggtc gatataaccc
     1681 gctccccgcc cctccataag tgcgacttta gccgtctgcg ccactccgga cgcggagtga
     1741 agctccatat aagcgatgct gctcaactcc gagggagtcc gggtagcagc atccatacag
     1801 agtgccgagc accatattct ttctccggct atatatatgt ttctatcagt ggaaaaccag
     1861 actctctcca ctcgctgtgc gaatagtgga gcagataaaa gcagcaaaac tcctgtaagc
     1921 agtgtaatta tagtgaagtc tcttttcatc ctttaattgt ggttattcgg ccaataatcg
     1981 ggtttatctt tagtgcctcc cttcacccta cagtctacac aacgcctctc ggaccagctg
     2041 gtaatgctcg tcatctcatc gaggaatacc acgtcatagc ctccgaaata ataccttctc
     2101 atttttgact cctctgcgag gtttatttct atgaaagctg aacaattttc gcgtctgttt
     2161 tgcatgggaa agtcatttac cgagacaaat attctgcggc ttgttacctt tgatgcagag
     2221 atatagccca gcacaacctt ggtggagtca cctacacaat ggatattacc cctgagttcg
     2281 cttggctgag gagagaaaag tcctcccggt tgatctgtat tacgcaacat aacctcccag
     2341 tagatatagg cttccgccga aatggagctc tggatcacct ctattgagta taaataatca
     2401 agcttgtcgc tggcaaaggg aagttgatag atttgatgat ttacaagacg attctctgat
     2461 agcgcatctg tgcttgcaat cataatatcg ggaacaacat ccctgtccca gcagtaatgt
     2521 ctgttctcat tggggctttc ctcaacggta ttacgattcg gattaaacac gtgtgaagca
     2581 taaaattgag aagtatactc ccaaacctca ttacagttcc acttataata gggattggtg
     2641 tccggattgg tggtactgac atgaaatgtt attttctctt cgagttcgcc aaccctgaat
     2701 gagacctctt cgatttccgg agtcacaagt acctccaacc agtcggttac atactccgtg
     2761 ttatagccag gtgaggcaat atatcccata ccactataag gcttgacatg gagacgataa
     2821 gaagcccggg ggtcaagatt gcgggtatcg ataatgtggg caccatcccg gtaatttgag
     2881 gcacgggtta tctctccctt atcattttcc acccacacat ctgccatgat atctacggga
     2941 ctaccggtag cggtcaggcg ttgagtgttg cggacaaata tatatgattc ggttccgatg
     3001 cggatgtctc cctctattac aaatagtccc aaatctaaat cctctatctc cggtgtaaag
     3061 ggatagatgc aacttccggt caggaacgct acaaaagcga gaattagtat gcaaagtcgt
     3121 tctttcatca gaatttgagg ttgaggttga tatagggtat cggcactgca aaaacacaaa
     3181 gcatattgcc actgacgctt ccactaccgc tgcgggtgta aaacaccgaa tatggatttt
     3241 ttcgggcagt cacattgtag actccgaagg tcacggagag atgcgaaagc tggcgaaggt
     3301 agtggctggg ttcaatattt atcgccagat cgaggcggaa atagtccggt attcgataac
     3361 cgttgcgcac actataatag agacggtagc ccccgccata aaggtattta gccacaggga
     3421 tggttacggg acgaccagtg gagtagtcga gattgaccga tatgctatat ctgtgggtaa
     3481 atttgtagtt gcccaccagt ttgagatcgt gcggtttgtc gtgcggcgca ttgtaccact
     3541 ctccatcatt gatggtttgc agacctctgt cctccatttc ccgaagcaat gatctggagt
     3601 aggtgtagga gacccaaccg ttgagttttc cacccggctt tttaaccata aattcgactc
     3661 cgtaggctct ccccatggta gaaaccagtt catctgcaag gttttcattc atcacgagga
     3721 tggctccgct cttgtagtcc ggatagtcgc gcatcatctt gtaatagcct tcaacagaga
     3781 ggtccacccg atggtcaaat accgtccagt agagtcctgc cgcagcctgc cagccctttt
     3841 gtggcccgat atcggcgtca gcaagcttcc atgtatctgt gggagaaatg gctactgtat
     3901 tagatatttt atggatgtat tgagccatac tgttgaatcc cgcttttaag gagagatttg
     3961 ggaggaatga atattttccg gagagtcgca actcaggtgt cccgtaaaat ttacgggaat
     4021 ttaagcttag aaaaccggaa agtctcaatc cgaaatcaaa cgagagtcga tcggtcggct
     4081 gataggtatc attgaaaaaa agtgcagtct ccagagctcg ttccgtcacc aaacgacgcg
     4141 gttcaatagc tgaagaatct ccgtagggaa gtctgttgcc cggctccaca ttatacatag
     4201 tgctgttgaa gccgtaagaa aatgtatgtt tcgcagctac aagtgactga aatttaaggt
     4261 tgagataacc ctgcctgata ttgctggaaa gagaaaaaga gctccagtcg ttgtgagtat
     4321 cctccacctt atagccataa ctgtcatatc cggtcgaaag ggagagtgaa tgaccatcgc
     4381 tgaagtgatt tctccatttc aaggagaaat tggcattgtg atacctatat gtggtatcga
     4441 cagtaaaact aaacttgtct ctcgaaaagt aaccggaaaa atgtagggaa tttttctggt
     4501 tgaatctgtg actgatttga gcgttgaggt cattaaatga ggccgtcccg ttggaatatt
     4561 cgctgttttt tggaaggact ttcaatatcc aatcggaata ggtagtcctg ccaccgagaa
     4621 taaacgtggt tttctccttt tttatagggc cttcgagatg gaatctgctg gtaagaactc
     4681 ccagtccgag cgagccggat atcttcttgc tattgccttc acggcccctg atatccagca
     4741 ccgaagatat tcttccgcca tattcggcgg gaattgaact cttgaagagt tctacgtcac
     4801 tca
//
LOCUS       DAOSIE010000099         4756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_15952_length_4756_cov_3.647735, whole genome shotgun sequence.
ACCESSION   DAOSIE010000099 DAOSIE010000000
VERSION     DAOSIE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15952_length_4756_cov_3.647735"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLI69_03990"
     CDS             <1..818
                     /locus_tag="PLI69_03990"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG41683.1"
                     /translation="NLKSSDLTDKIHNIFTMEHKILYYGPAAGEKIVNLIAETHNVPE
                     SLTPVVSGNPYKIAKTEGTKVFLAPFDANQLYYLAVSNIGQEYDPAIVPITNLYNSYF
                     GSGMNSIVFQEMREARGLAYSANARVYLPTDLEHETFFASFIATQNDKLIDAMTAFDE
                     IINNMPVSQNAFDIAQESLISNLRTARTVRDNVLWQYISAQKLGLDYDINREVFAKAQ
                     TLTLDDVVKYQQENIKDRYYTICILGRYEDFDMKRLLEYGPIQKLELKEIFGY"
     gene            909..1076
                     /locus_tag="PLI69_03995"
     CDS             909..1076
                     /locus_tag="PLI69_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG41684.1"
                     /translation="MAYKITENCAACGSCIDECPVGAISEGDIYKIDPNLCIDCGACA
                     DACPLGAIVQA"
     gene            1254..4550
                     /gene="secA"
                     /locus_tag="PLI69_04000"
     CDS             1254..4550
                     /gene="secA"
                     /locus_tag="PLI69_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOG41685.1"
                     /translation="MGLSDIIKKLFGSKSERDLKQLKPILNNIHNAYSRIDALSDDDL
                     RNESAAIRAKVLDYVAVEEAKVVSIREQLEDVEIAVSEKEKLATELDLLIKEIDTKLE
                     DILNQVLPETFAIVKSTARRFKDNPVIRVKATQFDRDLSTTHDFVTIKGDIAEWKNSW
                     IAGGNMITWDMVHYDVQLYGGAVLHAGKIAEMATGEGKTLVATLPVFLNALAGKGVHV
                     VTVNDYLSKRDSEWMGPLYMFHGLSVDCIDKHQPNSDARKRAYNADITFGTNNEFGFD
                     YLRDNMALNPDDLVQRKHHFAIVDEVDSVLIDDARTPLIISGPVQRSGDQTFIEYKPY
                     VQRLYNEQRTFVNQTLNEAKKLIAAGEIKDKGEILLLRAHKGLPKYAPLIKYLSEPGI
                     KQILQDVQSKIIKAAFTEKDIEQRIVTNDLFFVIDEHQRSVELTDKGNEVLAKAVGDA
                     DFFLMPRLGDELSKIDQMDIPTEEKRQKKDELYSNYSLKAERMHTVDQLLKAYSMFEK
                     DVDYVVIDNKVKIVDESTGRIMEGRRWSDGLHQAVEAKENVKVEAATQTFATITLQNY
                     FRMYHKLSGMTGTAETEAGEFWSIYKLDVVVIPTNRPIIRDDQDDLIYKTKKAKYNAV
                     IEHIGELVKEKRPVLVGTTSVEISEYLSRLLKLHGIDHQVLNAKQHAREAEVVLHAGK
                     PGTVTIATNMAGRGTDIKISDEVKEAGGLAIIGTERHDSRRVDRQLRGRAGRQGDPGS
                     SIFYVSLEDDLMRIFGSDRISKVVDSMGMQEEEALQASMLSNAIERAQKKVEENNFGT
                     RKRLVDYDDVMNSQREVVYTKRRSALTGERIDVDVLNMISDYCEMFADSNRHLTYDLF
                     SEELMKVLSIFPDFDEASYERFNKAQLTEQLQQSVQNVMQRRFDTLIEQTWPVISHVY
                     DTKRMFYQNIQIPVSDGKMVYGILVDLRKAYESKGKELIRAVQKTITLRVIDEYWKNH
                     LRDMDDLKQAVQHAAYEQKDPILVYKGESFELFIKVLEGISRDVLAFLLRSFLFLRND
                     QQPQEVATEGHTKKTDLSGMRESRPDLLTNSSEPQSNAPARAEKKVGRNDPCPCGSGK
                     KYKNCHGKNQ"
     gene            4572..>4756
                     /locus_tag="PLI69_04005"
     CDS             4572..>4756
                     /locus_tag="PLI69_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG41686.1"
                     /translation="MEKFDVIVIGSGPGGYVAAIRASQLGFNTAVVEMSELGGICLNW
                     GCIPTKALLKSAQAYNYA"
BASE COUNT         1464 a         1053 c         1104 g         1135 t
ORIGIN      
        1 aaaatctaaa aagcagcgat ctcactgaca aaatccacaa catcttcacg atggagcaca
       61 aaatactcta ttacggaccc gccgcaggag aaaaaatagt gaatctgatt gccgagaccc
      121 acaatgtgcc tgaatccctt acccctgtcg tatcggggaa cccctataaa atagctaaga
      181 ccgaaggcac caaagtcttc cttgctcctt tcgatgccaa ccagctatat tatttagcgg
      241 tatcgaacat cggacaggag tacgatccgg ccattgtccc cataacaaac ctgtacaact
      301 cctatttcgg ctccggaatg aactccatag tattccagga gatgcgtgaa gcacgcggtt
      361 tggcttattc tgcaaacgcc agagtatatc tgcctacgga tttggaacac gaaacatttt
      421 tcgccagttt catcgccaca caaaatgaca agcttattga tgcgatgaca gctttcgatg
      481 aaatcatcaa caatatgccg gtgtcacaaa atgcatttga catagcccag gaatcactaa
      541 tcagtaatct ccgtacagcc cgcacagtta gggataatgt tctatggcaa tatatcagtg
      601 cgcaaaaact tggtctggat tatgacatca accgcgaagt atttgcaaag gcacagactc
      661 taactctcga tgatgtggtg aagtaccagc aggagaacat caaagaccgt tactacacca
      721 tctgcattct cggccgctat gaagattttg atatgaagag actcctggag tacggtccca
      781 tccaaaaact cgagctcaaa gagattttcg gatattgata taaataattt gattaatccg
      841 ataaaaagat gttatctttg cggacacaac tgatgattga aactatcaaa taactaaaaa
      901 aattaaacat ggcttacaaa atcactgaaa actgtgcagc ttgcggttct tgtattgacg
      961 aatgtcctgt aggagctatc tctgaaggcg atatctacaa gattgacccc aacttgtgca
     1021 ttgattgcgg tgcttgtgca gatgcttgtc ctttaggagc tatcgtgcag gcataattaa
     1081 ttgcaacgac catacaacaa agggcacctg cgggtgccct ttgttgattg tgacagaggg
     1141 atctagcaga tatccacaga taaaagccgt tcaaacgttg tagcagggaa aataaagtga
     1201 aatattagta ttttattcgt agatttgcaa gattaatctt gttttgtaaa aatatgggac
     1261 tttcggatat aattaaaaag ctgttcggat ctaaatcaga aagagatctg aagcagctaa
     1321 aacctatact taacaacata cataacgcat atagccgtat tgacgctctc tcagacgatg
     1381 acctcaggaa tgaatccgct gccatcaggg caaaagtttt agattatgtt gcggttgaag
     1441 aggcaaaagt ggtctccatc agagagcagc ttgaagatgt ggaaatcgcc gtttcggaga
     1501 aagagaaact tgctactgag cttgacttgc ttatcaaaga gatagatacc aaacttgagg
     1561 atatactcaa ccaggtactc cctgaaactt ttgccatagt aaaatctaca gccagacgat
     1621 ttaaagataa tcctgtaata agagtcaaag ccactcagtt tgatcgcgat cttagcacca
     1681 cccacgattt tgtcaccatc aaaggcgata ttgccgaatg gaaaaattcc tggatagcag
     1741 gtggtaatat gattacctgg gatatggtac actacgatgt acagttgtac ggcggtgctg
     1801 tcctccacgc aggtaagatt gccgagatgg ctacgggtga gggaaaaact cttgtggcaa
     1861 cactccccgt cttcctcaac gctcttgcag gcaaaggtgt tcatgttgtg acagtcaacg
     1921 attatctctc caaacgtgac tccgaatgga tgggccccct ctatatgttc catgggctaa
     1981 gcgtagattg tattgacaag caccagccca attccgatgc aagaaaacgt gcctacaatg
     2041 ccgatatcac cttcggtacc aacaacgaat ttggtttcga ctaccttagg gacaatatgg
     2101 ctctcaatcc cgatgacctt gttcagcgca aacaccattt tgccatagtc gatgaggtgg
     2161 actcggtcct gatagatgat gcacgtactc cgttgatcat ttcgggaccg gtacaacgta
     2221 gcggagacca gaccttcatt gagtacaaac cttatgtgca gcgtctctac aatgagcaac
     2281 gcacatttgt aaaccagacc ctgaacgagg caaagaaact cattgccgca ggcgaaatca
     2341 aagataaagg ggaaattttg ctactcagag cacataaggg actccccaaa tatgcacctc
     2401 tcattaaata tctcagtgag cccggtatca aacagatttt gcaggatgtg cagagtaaaa
     2461 taataaaggc cgccttcact gaaaaggaca tagaacagag aattgtcacc aacgatctgt
     2521 ttttcgtcat agacgaacat cagcgcagcg ttgaactcac cgacaagggc aacgaagtac
     2581 ttgccaaagc cgtaggtgat gctgatttct tcctgatgcc caggcttggc gacgagctct
     2641 caaagattga ccagatggat atccccaccg aggagaagag acagaaaaaa gatgagcttt
     2701 actccaatta ctccctcaaa gccgagagga tgcacacggt tgaccagctc ttgaaggcat
     2761 attcaatgtt tgagaaggat gtggattatg tggtaattga caataaggta aagattgtag
     2821 atgagagtac aggccgtata atggaagggc gtcgctggag cgatggcctc catcaggctg
     2881 tggaggccaa ggaaaatgta aaagtcgaag cagccacaca gactttcgcc accattaccc
     2941 tccagaatta cttcaggatg taccataaac tttcgggaat gacaggtacg gcagagacgg
     3001 aggccggcga gttctggagc atctacaagc tcgacgtagt ggtcatacct actaaccgtc
     3061 caattatcag agacgaccag gacgacttga tctacaagac taagaaagca aaatataatg
     3121 cagtcataga gcacattggt gaactggtta aggaaaaacg ccctgtactc gtaggaacca
     3181 cctctgtgga aatatcagag tatctgagcc gtctgttgaa attacacggc attgatcacc
     3241 aggtgctcaa tgccaagcaa catgcacgtg aggctgaggt tgtactccac gcaggtaagc
     3301 ccggtaccgt gaccatagcc accaatatgg cgggacgtgg taccgacatt aagatatcgg
     3361 atgaagtcaa ggaagccgga ggtcttgcaa tcataggtac cgagcgtcac gatagccgcc
     3421 gcgttgaccg tcagctcaga ggacgtgcag gacgtcaggg ggacccgggt agctccattt
     3481 tctatgtatc tctcgaagat gacctgatgc gtatcttcgg ttccgacagg atttccaagg
     3541 tagtcgattc gatgggtatg caggaagaag aggcacttca ggcctctatg ctctcaaatg
     3601 ccatagagag ggctcaaaag aaggttgagg aaaataactt cggtacgcga aagagactcg
     3661 tagactatga tgacgttatg aactcacaga gggaggttgt ctataccaag agacgcagcg
     3721 ctctgaccgg tgaacgcatt gatgtggatg tactaaacat gatttcagac tattgcgaaa
     3781 tgtttgcaga ctccaaccgt catctgactt atgatctctt ctctgaagag ttgatgaagg
     3841 tgctttccat ctttccggat tttgatgaag cctcctatga gcgtttcaat aaggctcaac
     3901 tcacggaaca acttcaacaa agtgttcaaa atgtgatgca gcgcagattt gacaccctga
     3961 ttgagcaaac atggcctgta atcagccacg tatatgacac aaagaggatg ttttaccaga
     4021 atatacaaat ccccgtatcc gatggcaaga tggtatatgg catactcgtg gatctgagga
     4081 aggcttatga gagcaaaggc aaggagttga ttcgtgccgt acagaagaca attaccctaa
     4141 gagttattga tgagtactgg aaaaaccatc ttcgggatat ggatgacctt aagcaagcag
     4201 tacagcatgc cgcttatgag caaaaagacc ccatacttgt ctacaaaggg gagagtttcg
     4261 aactcttcat caaggtgctt gagggcatca gcagggatgt tcttgcattc ctcctgcgtt
     4321 cattcctctt ccttaggaat gatcaacaac cgcaagaggt tgccacggag ggccatacca
     4381 agaaaaccga tctgagtgga atgagagaga gccgccccga tctgcttacc aacagttcgg
     4441 agccccaatc aaatgctccg gccagagcag agaaaaaagt tggccgcaat gacccatgcc
     4501 catgtggaag cggtaaaaaa tataagaatt gtcacggaaa aaatcaatga gaaaaatata
     4561 atccaaataa catggaaaaa tttgacgtca tagtaatagg ttcgggaccg ggagggtatg
     4621 ttgcagcaat ccgtgcttcc cagttaggat ttaacacggc agtagttgaa atgagtgagc
     4681 ttggcggaat ctgccttaac tggggctgta tacccacaaa agcccttttg aagagtgctc
     4741 aggcatataa ttatgc
//
LOCUS       DAOSIE010000100         4627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_16488_length_4627_cov_4.469598, whole genome shotgun sequence.
ACCESSION   DAOSIE010000100 DAOSIE010000000
VERSION     DAOSIE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16488_length_4627_cov_4.469598"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..370)
                     /locus_tag="PLI69_04010"
     CDS             complement(65..370)
                     /locus_tag="PLI69_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG41687.1"
                     /translation="MNTKENMIQDYDVVLDNLYGKEGTPERNRFEENAYAYYSGQILR
                     NARKEAKVTQAELAERSNTSKSYISRIENGTINPSVGVFYKLIAALGLNVEIVKTIS"
     gene            complement(336..683)
                     /locus_tag="PLI69_04015"
     CDS             complement(336..683)
                     /locus_tag="PLI69_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG41688.1"
                     /translation="MRRKIIIFGKYFTEFINKLTIKEREKVDYGLVLLKTQDRLPAKF
                     VKYIRDGLYELRTEYQGNIYRVFFIFDTGKIVVLFNGFQKKTQKTPVKEIEKALRIKE
                     EYYEYKREHDTGL"
     gene            899..2242
                     /locus_tag="PLI69_04020"
     CDS             899..2242
                     /locus_tag="PLI69_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG41689.1"
                     /translation="MKKIIITVAAILCSGLLYAQEVMTLKECMNYAVENSSRVKIQQM
                     EVDDARVARRDALLNIFTPEISAGTYAYSNFGRSVDPETNTYVSTTSFNNVYALSGGI
                     TLFNGFASVNNAKISKTALKMGRDQEALIKDEVCLATMEAYYNVVYFGQLAELLENQV
                     KTAKNSLKLAQKQEELGQKSYADVVEMQAELYDREYKLVMAGNQYEDALLKLKDLMFW
                     PLDEELIIDASMVIPENVRTLIAEEETEELVDYAIHSLPSIAIAKGMMENAKIDYNTA
                     KWRFSPRLSLQGGWATSYYTYPRQKDYIPLPFETQFRNNANQYVQLSLTFPIFNRLST
                     FSNIRKKKNAYDRATVQYEQKLRDVEAEVKRAVQDRDGTYAAYEQADCMSRAQEEAYR
                     LNVRKFEQGLISTIDFQKASDNWLNAKTSRLDALLKFYIKKSVVDYYGGIGYLEQ"
     gene            2256..3512
                     /locus_tag="PLI69_04025"
     CDS             2256..3512
                     /locus_tag="PLI69_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOG41690.1"
                     /translation="MDKIIEKKKGIQVAFTKKALPYWLGALLVGFIVWLLVRNNSSTL
                     RVNPETLFISEVKSGEFNDYIRISGQVQPMTTIQVSPQEGGIVQEIVIEEGSLVKAGD
                     VILRLSNDNLDLQILNSESELAEKENILRNTLIAMEQQRLNVQQEKLQLEIDVKKSLR
                     KYQQNKALYEEKLISREEYLVAKEDYELASGRLELVKERAEQDSLYRGVEVRQMEESL
                     RNMRLNMYMIRKRKDNLMIKSPIDGELGLLDVVLGQSIASGTKIGQINELGSYKIEAQ
                     IDEHYIDRVTAGLEASFERQNEAYDAVIRKVYPEVRDGKFKADFKFSGEQPANIRTGQ
                     TYYLNLQLGQPEKAIIIPRGTFYQKTGGKWIYVVSPEGGKAVKREIRIGRQNPQYFEV
                     LEGLEAGEKVIISGYDTFGDNDVLVF"
     gene            3523..>4627
                     /locus_tag="PLI69_04030"
     CDS             3523..>4627
                     /locus_tag="PLI69_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG41691.1"
                     /translation="MKIKEIITKALSLGTGLAIGILLIANISYELSHDRCYTEPERIY
                     QIRSLFFENGKESDYHNVSGAIAPGFKEYIPGVEYATRMTPLWNSDKFSDENKNIISG
                     SIIAADTSFFDVFDTEILTGNPKDALSQRGSAMVSESFADKLGGATEALGKIIFNEES
                     PNFPITINGVFADFPHHSSVKRDVLLSMESLRKESTDNWLGNDRYGGFVRLMKGTDPD
                     MLAPAIRDMQAKNYPAEAIKSFQESGLDLQYYLAPLNRQYLKSEQVRITLLILSIVAV
                     LLIAISLLNYILFSVSAISNRSKEMGVRKCYGAGQRNIYGIMFKEAVVDVLAAILVAT
                     IIILFFRPLIEDITGVPTSTLLVSARYTALALIL"
BASE COUNT         1339 a          939 c         1058 g         1291 t
ORIGIN      
        1 accgccgccc tgaagatgga atatatatag ttccaatagg aacattaaaa gattaattct
       61 tgaattatga aattgtttta acaatctcaa cattcaatcc aagcgctgct atcaacttgt
      121 aaaatacacc aacgctcgga ttaatcgtcc cgttttctat tctggagata tatgatttgg
      181 aggtattgga gcgttctgca agttcggctt gagtgacttt tgcctctttt ctggcattac
      241 gcaatatttg tcctgaataa tatgcatacg cattttcttc aaatcggttg cgttcgggag
      301 tcccttcctt tccgtagagg ttgtctagaa ctacatcata gtcctgtatc atgttctctt
      361 ttgtattcat aatattcctc cttgattctt aatgcctttt ctatctcttt tacaggtgtt
      421 ttttgtgttt tcttttgaaa tccattaaat aatacaacta ttttccccgt atcaaaaatg
      481 aaaaacaccc tgtatatatt tccctgatat tctgtcctta actcgtaaag cccatccctt
      541 atgtacttga caaacttggc gggcaacctg tcttgtgttt ttagtaaaac cagaccatag
      601 tcaacctttt ctcgctcctt aatcgtaagt ttatttataa actcagtaaa gtattttcca
      661 aagattatta tctttctcct cattctttct tctccttaat ctttaattgc aaagatacaa
      721 aaagtttcaa gatattgaaa ctttttgtat cctattctgt ccactccttt tacacttctg
      781 taaagtttct ttacacttat attttggcga aaaaatcaca aagcactatt tgtcagtgtg
      841 ttgaccgtcg tggcacggtg ggtgctttca tttgaggtga agtgaaaatt ttatcaaaat
      901 gaaaaaaata ataatcaccg ttgcagcaat tctttgttcg ggacttctct atgcgcaaga
      961 agttatgacg cttaaggagt gtatgaatta tgctgtggaa aactcctcca gggtgaaaat
     1021 ccagcagatg gaggtagatg atgcgagggt ggcccgtcgg gatgcactct tgaacatctt
     1081 tactccggaa atttcggcgg gaacctacgc atattccaat tttggccgtt cagtcgaccc
     1141 cgaaaccaac acctatgtgt cgactacctc ttttaacaat gtgtatgcac tttccggagg
     1201 tattacgctc ttcaacggtt ttgcttcggt aaataatgcg aagatcagca aaactgcact
     1261 gaaaatggga agggatcagg aggctttgat taaagatgag gtatgcctcg ccactatgga
     1321 ggcctattat aacgtggtct acttcggtca gcttgcagag cttctggaga accaggtgaa
     1381 gacggctaaa aactctttga aacttgcaca aaagcaggag gagctgggac aaaagagcta
     1441 cgctgatgtc gttgagatgc aggcggagct ttatgatagg gagtacaagt tagtgatggc
     1501 cgggaatcaa tatgaagatg cgcttctcaa gctcaaagat ctgatgtttt ggcctctgga
     1561 tgaggagttg attatcgatg cctcaatggt tattcccgaa aatgtaagga ctctcattgc
     1621 ggaggaggaa acagaggagt tggtagacta tgctattcat tcgcttccct ccatagctat
     1681 cgctaaagga atgatggaga atgcaaagat cgactacaat actgcaaaat ggagatttag
     1741 ccccagactt tcgctccagg gcggttgggc taccagctat tatacctatc cccgtcagaa
     1801 ggattacatt cctcttccct ttgaaaccca attcaggaac aatgccaatc aatatgtaca
     1861 actttctttg acattcccga ttttcaacag actctccact ttttccaata tcagaaagaa
     1921 aaagaatgct tacgacagag ctaccgttca atatgagcag aagctgcgag atgtggaggc
     1981 ggaagtaaaa agagccgtac aggacagaga tggcacttat gcagcctacg agcaggccga
     2041 ttgcatgtca agggcacagg aagaggctta tcgtctgaat gtgagaaagt ttgaacaggg
     2101 tcttatctct acgatcgatt ttcaaaaagc atcggacaat tggctcaatg caaagacaag
     2161 caggttggat gcactcctga aattttatat aaagaaaagc gtggtggatt actacggcgg
     2221 tataggttat ttagaacaat aaaaaatcaa ataaaatgga taagattata gaaaagaaaa
     2281 agggcattca agtggctttc actaagaaag cactccctta ctggctcggt gccctattgg
     2341 tgggatttat cgtatggctc ctggttcgca acaattcaag caccctgaga gtcaaccctg
     2401 aaacactctt catcagcgaa gttaagagcg gtgaattcaa cgactacatc cgtatcagcg
     2461 gacaggttca acctatgact accatacagg taagcccgca ggagggtggt atcgtgcagg
     2521 aaattgtaat tgaagagggt tctttggtga aggcaggtga tgtgattctt cgtctcagca
     2581 acgataacct cgaccttcag atcctcaatt cggaatccga gctcgccgag aaggaaaata
     2641 tccttcgcaa taccttgatc gcaatggagc agcaaagatt gaacgtgcag caggagaagc
     2701 tccagcttga aattgatgta aaaaaatcct tgcgtaaata tcaacagaac aaggcacttt
     2761 atgaggaaaa actgatttcc cgtgaggaat acctggtggc aaaagaggat tatgagctcg
     2821 cttcgggcag acttgagctg gtgaaagaga gggctgagca ggatagcctc taccgtggcg
     2881 ttgaggttcg tcagatggag gaaagtctca ggaatatgag attgaatatg tatatgatcc
     2941 gcaaaagaaa ggataacctg atgataaagt cgcctattga tggagaactc ggacttctgg
     3001 acgtggtgct gggacaatct atcgcaagcg gtacaaaaat aggtcagatc aacgagctgg
     3061 gcagctataa gatagaggca cagatcgatg agcactatat cgacagagtc acagcaggcc
     3121 tggaagcgag ctttgagagg caaaatgagg cctacgatgc cgttatccgc aaggtgtacc
     3181 ccgaagttcg tgacggcaaa ttcaaagccg acttcaaatt cagtggcgaa cagcctgcta
     3241 atatccgcac cgggcagacc tattacctca atcttcagct cggccagccg gagaaagcca
     3301 tcatcatccc tcgaggtaca ttctatcaga agaccggagg caaatggatc tacgtagtct
     3361 ctccggaggg aggcaaagcg gttaaacgtg agatccgcat tggccgtcag aatccccaat
     3421 attttgaggt tttggaaggg ctcgaggcgg gtgagaaggt gatcatttca ggttacgata
     3481 ctttcggaga caatgatgtg ttagtattct aaaaagagag caatgaaaat taaggaaata
     3541 attacaaaag cactgtcgct cggtacgggc ctcgccatag gaatcctgct tatagccaat
     3601 attagctacg agttgagcca tgacaggtgt tatacggagc ctgaacgaat ttatcagatc
     3661 cgctccctat ttttcgaaaa tggaaaagag agtgattatc acaatgtttc cggtgcaatt
     3721 gctcccggtt tcaaggaata tattccggga gtggaatatg ccacaagaat gactccttta
     3781 tggaatagcg ataagttttc cgatgaaaat aaaaatataa tcagcggttc cattatagct
     3841 gccgatacat ctttctttga tgtctttgat acggaaattt tgacgggaaa tccaaaagat
     3901 gctctttcgc aaaggggctc ggctatggtg tctgaaagtt tcgctgacaa gcttggtggc
     3961 gctactgaag ctttgggtaa aatcattttt aatgaagagt cacctaattt ccctattacc
     4021 attaacggtg tatttgccga tttccctcat catagttctg taaagcgcga cgttctgctc
     4081 tcaatggaga gtcttaggaa ggaatcgacc gataattggc tggggaacga tagatatggg
     4141 ggttttgtgc ggctgatgaa gggcacggat cccgatatgc tcgcacctgc catcagagat
     4201 atgcaggcga aaaattatcc cgccgaagcg attaaatcct ttcaggagtc cggtttggat
     4261 cttcaatact atctggcacc gttaaacaga cagtatttga aatcggagca agtcagaatt
     4321 acactcttga ttctttcgat tgtggctgtg ctgttgattg ctatttcgct gctgaactat
     4381 atcctcttct cagtttcggc tatctccaat cgctccaaag agatgggagt gagaaaatgt
     4441 tatggagcgg gccagaggaa tatttatgga ataatgttta aagaggctgt agtggatgtc
     4501 ctggcggcca ttttggttgc aaccataata atccttttct tcagaccttt gatcgaagat
     4561 attacaggcg ttccgacatc tacactcctt gtaagtgcta gatatacggc actagctttg
     4621 attctca
//
LOCUS       DAOSIE010000101         4557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_16791_length_4557_cov_4.645047, whole genome shotgun sequence.
ACCESSION   DAOSIE010000101 DAOSIE010000000
VERSION     DAOSIE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16791_length_4557_cov_4.645047"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLI69_04035"
     CDS             complement(<1..529)
                     /locus_tag="PLI69_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOG41692.1"
                     /translation="MDNERKVIEEIAGGEYRHGFETDLEQEFIPKGLNEDIIRIISAK
                     KEEPEWMLDFRLKAFAKWQQKSMPTWAHLEIPEIDFQDIIYYAAPKITNSSGEIDPEL
                     AATFDKLGIPLHERNVLGGVAVDAVFDSVSVKTTFRETLAEKGIIFCSFSEAVRDYPD
                     LVRKYLASVVPIGDNY"
     gene            complement(685..1896)
                     /locus_tag="PLI69_04040"
     CDS             complement(685..1896)
                     /locus_tag="PLI69_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG41693.1"
                     /translation="MNLSTFIAGRYLFAKKSHNVINIISGISAAGIAIGCAALVVIMS
                     IYNGFDSIIRTLYNSYTPDLLITPAEGKVFTTESEGFEKIRSDASVLSFCEVLEENLF
                     VRYGDSHIVATARGVDSVYISATGLKDYVVEGSFELEFGDLKQVVLGHTLAQELGLKI
                     AFVSPLEVYFPSRTADVSPVNPFASLRSQRLYPGGIISLDQNFDKKYMFMPVESLRSL
                     LDYDKEVTSVEIYVDSSALSPNGVITADFQKRVSAALGDSFTVKNRQQQNESLYKLLS
                     YEKIAIYAILLFVMIIISCNIFGSLSMLIIEKRDDIEILKSMGADERLIKKIFVKEEW
                     MISLLGIAAGIALGLLVCLLQQRVGLVRMPGNFIVEYYPVVIRWSDILVIASGVGLIG
                     YLAAVVSRAAN"
     gene            complement(1896..2243)
                     /gene="rbfA"
                     /locus_tag="PLI69_04045"
     CDS             complement(1896..2243)
                     /gene="rbfA"
                     /locus_tag="PLI69_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOG41694.1"
                     /translation="MENESKRQRMVARQIQRDLAEIIRGRGMAAFDGAMVTVSGVKMS
                     PDLSLARVYVSIFPSDKSQKVMDTLSLQNRSLRGELGNLVAKQLRIVPELTFFIDDSL
                     DYVEHIDELLNKE"
     gene            complement(2256..3560)
                     /gene="tyrS"
                     /locus_tag="PLI69_04050"
     CDS             complement(2256..3560)
                     /gene="tyrS"
                     /locus_tag="PLI69_04050"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOG41695.1"
                     /translation="MVKISKNFVKELEWRGMIHDMMPGTEEQLMKEPTSAYVGIDPTA
                     DSLHIGHLVSIMMLKHFQNCGHRPIALVGGATGMIGDPSMKSSERNLIDEATLRHNEA
                     AIKRQLSKFLDFDSEAPNAAKLVNNYDWMKDFTFLDFIRDIGKHITVNYMMSKDSVRK
                     RISGDEREGMSFTEFTYQLVQGYDFLWLYEHEGCKLQMGGSDQWGNITTGTELIRRKL
                     SGEAFAMTCPLITRADGGKFGKTEQGNIWLDARYTSPYTFYQFWLNVSDEDAQKYIRI
                     FTMLTREEVEAAVAEHLQAPHQRNLQKLLAKEITVMVHGEQEYVNALNASSILFGNAT
                     SEALKALDEQTFLSVFEGVPRFELRKEQLPSDIIEMLAVTTSVFPSKGECRKMIQGGG
                     LSINKEKVTETDFLLSKEHLLDDKYILVQKGKKNYFILKFEQ"
     gene            complement(3686..4324)
                     /locus_tag="PLI69_04055"
     CDS             complement(3686..4324)
                     /locus_tag="PLI69_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOG41696.1"
                     /translation="MALFLKKELDNDAQIFVWEVTESEEQLMALSAPIPKDELEDLSF
                     IKSEARRREKLAVRALLNEIFEDKVYLGHHDNGRPYLQNMATEISIAHTNRFVAIIIH
                     PDETLGIDIESLDRDFSAVEKKALSEDEVEDLSERNKNLHLAIYWCAKEAIYKRMSLH
                     GVDFAEQIELERFSPDDEGELEATFTHKDGVEMEFDLEYMVFENHVMVWVVG"
BASE COUNT         1192 a         1155 c          963 g         1247 t
ORIGIN      
        1 aatagttatc acctatcggg acaaccgaag ccagatattt acgcaccaga tcggggtaat
       61 ccctgaccgc ttccgaaaag gagcaaaata tgattccttt ctccgcaaga gtctccctga
      121 aggtggtctt gactgagacg gagtcgaata cggcatccac cgccactccg cccagcacat
      181 tgcgctcatg tagcgggatc cccagcttgt caaaagtagc tgccagctca ggatcaatct
      241 ctccggagct attcgtaatc ttcggagcag cataatatat gatatcctgg aaatcaattt
      301 cgggaatctc aagatgggcc caggtgggca tgctcttctg ctgccatttg gcaaaagcct
      361 tgaggcgaaa atccagcatc cactcaggtt cttccttttt ggcggagata atgcggatta
      421 tatcctcgtt caatcctttg ggaataaatt cctgttccag gtcggtttcg aagccatgcc
      481 ggtactctcc accggcaatt tcttctatga cttttctctc gttatccatt atttttaaaa
      541 tatttcaact tttttgcaaa aatgatgcaa cgtttttacc ctttattgca tctatcatct
      601 atatagtagg tttaggtttt agtacacaag gaaaggggtt tgattccggc agagggtcag
      661 cccttttttt tttacatata cctatcagtt ggctgctcgt gaaaccaccg cagccagata
      721 acctatcaat cccacaccac tcgcaataac caaaatatca ctccagcgta tcactaccgg
      781 gtaatattca actataaaat tgccgggcat cctgaccaat ccgactctct gctgcaatag
      841 acagaccaaa agccccagag ctatgccggc tgcaatgccg agtagcgaaa tcatccattc
      901 ctccttgaca aaaatctttt ttataagcct ctcatcggct cccatcgact tcaatatctc
      961 tatatcgtcg cgcttctcta taatcagcat cgaaagcgag ccgaagatat tgcacgaaat
     1021 tataatcata acaaagagca gtatggcgta gatggcaatt ttctcataag agagaagttt
     1081 atagagagac tcattttgct gttgccggtt tttaaccgta aaagagtcac ccaatgctgc
     1141 acttaccctc ttctgaaaat ccgcagttat cactccattc ggcgaaagag cgctggaatc
     1201 gacatatatc tcaaccgaag taacctcttt gtcataatcc agcagggagc ggaggctctc
     1261 aaccggcata aacatgtact tcttgtcaaa attttggtca agagagatga tgccgccagg
     1321 atagagacgc tggctgcgaa gcgaggcaaa agggttgacc ggcgaaacat ccgcagtcct
     1381 ggaaggaaaa tagacctcca agggtgatac aaatgctatc ttcaatccca gttcctgcgc
     1441 gagcgtatgg cctaagacca cctgcttaag atcaccgaac tccagttcaa aactgccctc
     1501 aaccacataa tccttcaatc ccgtggcact tatatagacc gaatccactc ctctcgccgt
     1561 agcaacgatg tgactgtcgc catatctgac aaagagattt tcctccaaca cctcacaaaa
     1621 agaaagaacc gaagcatcgc tccttatctt ttcgaatcct tcactctcag ttgtaaagac
     1681 ctttccctct gcgggggtta taagaagatc gggggtataa ctgttgtaga gtgtgcgtat
     1741 aatggagtca aaaccattgt atatcgacat tatcaccacc agggcggcac aacctatggc
     1801 aattcccgcc gcagagatgc ctgatatgat attaatcaca ttgtgagact tctttgcgaa
     1861 gagatagcgc ccggcaataa aagtcgatag gttcattact ccttattgag cagctcgtct
     1921 atatgctcga catagtcgag cgaatcatct atgaagaatg taagctcagg cacaatcctc
     1981 aactgttttg ccacaagatt gcccaattct ccccgcagac tcctgttctg aaggcttagg
     2041 gtatccatta ccttttgact cttatctgaa gggaaaatgc tcacataaac tctcgcaagc
     2101 gagaggtcgg gactcatctt tactccactt accgttacca tggccccgtc aaaggcagcc
     2161 atacctctac cccttattat ttcagcaaga tcccgctgga tctgccttgc taccattcgc
     2221 tgtcttttgc tttcgttttc cataacctta tatttttatt gttcaaattt caatatgaag
     2281 tagttcttct tgcctttttg taccaaaata tacttgtcgt ctagcaggtg ttcctttgac
     2341 agcaagaagt ctgtctccgt caccttttcc ttattgatgg agagaccgcc gccctgtatc
     2401 atcttgcgac attccccctt ggagggaaat acggaggtgg tcaccgccaa catctcgatg
     2461 atgtcggaag ggagttgctc cttacggagt tcaaaccggg gtacgccctc aaataccgag
     2521 aggaatgtct gctcatcgag cgcctttaag gcctccgaag tggcgttgcc gaaaagaatg
     2581 gacgaggcat taagcgcatt gacgtattcc tgctctccat gcaccatcac ggtaatctcc
     2641 tttgcgagca gcttctgaag atttctttga tgaggagcct gtagatgttc ggctactgcc
     2701 gcctcaacct cttcacgggt gagcatcgta aaaatcctta tatatttctg cgcatcctca
     2761 tcgctcacgt tgagccagaa ctgatagaag gtataaggag aggtgtatct tgcatcgagc
     2821 cagatattgc cctgttcggt ttttccaaat ttgcccccgt cagcccttgt tatcagtggg
     2881 caggtcatcg caaaggcttc gccggaaagt ttgcggcgga tgagttccgt accggtggta
     2941 atattgcccc actggtcgct gccgcccatc tggagtttgc acccttcatg ctcataaagc
     3001 cataaaaagt cgtaaccctg taccagctga taggtaaatt cggtaaagga cataccctcg
     3061 cgctcatcac cggagatacg ctttctgacc gaatccttac tcatcatata attgacggtg
     3121 atatgctttc cgatatcgcg tataaaatcc agaaaagtaa aatccttcat ccagtcgtag
     3181 ttgttgacca gtttggccgc attgggggct tcactgtcaa aatcgaggaa tttggagagc
     3241 tgtctcttaa tggccgcctc gttgtgcctg agagtcgcct catctatgag attgcgctcg
     3301 gatgacttca tcgaggggtc gcctatcata cctgtggcac cacctaccag agctatcggc
     3361 ctatgaccgc aattctgaaa atgtttgagc atcataatgc tcactaggtg gccgatatgg
     3421 agtgaatccg cagtgggatc aatgcccaca taggccgatg taggctcttt catcaactgt
     3481 tcttccgtgc ccggcatcat gtcatggatc atccccctcc actccaactc cttgacgaag
     3541 ttctttgata tctttaccat attcttatct atgtttctat tgtcgattta acctacaaag
     3601 gtagttattt tatagagatt aaaaaaccgc tgatgccata aatgcaacag cggtcatatc
     3661 catttcgaaa ggtgcgggag agggactatc ccactaccca aaccatcacg tgattttcaa
     3721 aaaccatata ttccagatca aactccattt caacgccatc cttgtgcgta aaagtagcct
     3781 ccagctctcc ttcgtcatcc ggactgaacc tttccagctc tatctgctcg gcgaaatcga
     3841 ctccatggag agacattctc ttgtagatag cctcctttgc acaccaatag atggccagat
     3901 ggaggttttt atttctttcg gagagatcct caacctcatc ctcggagagg gctttcttct
     3961 ctacggcaga gaaatcacgg tcgagagact cgatgtctat gcccaatgtc tcgtcagggt
     4021 ggatgataat ggccacgaaa cggttggtat gcgcaatgct tatctcagtt gccatattct
     4081 gaagataagg gcggccgttg tcatggtgac caagatatac cttatcctcg aatatctcgt
     4141 tgagaagggc ccttactgca agcttctccc tcctgcgggc ttcgctcttg ataaaactca
     4201 ggtcttcgag ctcatctttt ggtatcgggg ctgatagagc catcaattgc tcttcactct
     4261 cggtaacctc ccagacgaag atttgtgcat cattgtcaag ttctttttta agaaaaagtg
     4321 ccatagttat gataaaggat aaggcatccg ccacctgtta gagcggggat aagtgaatta
     4381 aagtgatttg gattgcactg tgcgtggaag aaggcccgga agagctttca tccgtatatg
     4441 gaaattgaca actcggaggt tccaagacag cgcttttaat tggttaataa gttgagatac
     4501 caggattcga acctagacag acagaaccaa aacctgtagt gctaccatta caccata
//
LOCUS       DAOSIE010000102         4542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_16848_length_4542_cov_3.918654, whole genome shotgun sequence.
ACCESSION   DAOSIE010000102 DAOSIE010000000
VERSION     DAOSIE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16848_length_4542_cov_3.918654"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLI69_04060"
     CDS             <1..618
                     /locus_tag="PLI69_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41697.1"
                     /translation="TSVALQLGEYLSLNSMKSEAGLMVTDNPKETLYYESLSGIKPHI
                     SGSALHSLMQNWAKENSIDLGNLVIAKAILEFPFEYSGNYKALDNYPGNLFPCRRVRT
                     KKHTLYTPIDEIYDSYMSNGSLNRSLLSYKPDIGLYLQDLIKKESSELTDEDDLWIMQ
                     TISYTNNTTNVTTHYPDFTHYNVAKFNGTGCKRNPKLRIVYTLLK"
     gene            complement(698..1966)
                     /gene="fabF"
                     /locus_tag="PLI69_04065"
     CDS             complement(698..1966)
                     /gene="fabF"
                     /locus_tag="PLI69_04065"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOG41698.1"
                     /translation="MLVKRVVITGIGTINPIGDNPEDYFSNLDLGVSGSKLITRFDTS
                     LFKTKFACEIRDYSPERFGMDIKEARKHDRYTQYALIAADQAINDSRIPLNEENLGRT
                     GVIIGSGIGGIETLTQETMDYTEGQPPRFSPFLIPKIITNIASGSISIKYGFRGPNFS
                     TTSACTSSANAICVAASMIQLGKADIMITGGSEAPITVTAIGGFNSMRALSTNNDDYQ
                     TASRPFDATRDGFVIGEGAAIIVLEEYEHALKRGAKIYAEIIGYGQNADAYHITAPSP
                     GGEGASESMRIALEDAGISPDQVDYINTHGTSTPLGDIAELDAIKKVFGDVVYNVNLS
                     SSKSMTGHLLGAAGAVEALACIHAIRDGIIPPTINFREKDEAIDYSLNLTLNSAQKRN
                     VRVAMSNNFGFGGHNATIIFAAPGFRPFPR"
     gene            complement(1987..2223)
                     /locus_tag="PLI69_04070"
     CDS             complement(1987..2223)
                     /locus_tag="PLI69_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOG41699.1"
                     /translation="MEDITAKVKSIIVYKLGVEENEVQPDASFTNDLGADSLDTVELI
                     MEFEKAFGISIPDEVAEKISTVQDAIDYIENNQK"
     gene            2352..2918
                     /locus_tag="PLI69_04075"
     CDS             2352..2918
                     /locus_tag="PLI69_04075"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOG41700.1"
                     /translation="MTNLAIYASGSGTNAANIIRYFKNHPEISVKVVLCNNPEAFVIE
                     RAKRLDIPCLVFPKSGLAETINILKEYEIDYIILAGFLLKVPKNILEAYRDRIINIHP
                     ALIPSQYGGKGMFGMHVHKAVIAAGEKESGITIHLVDEIYDHGENIFQIRCPVNVDDT
                     PKDLADRIHCLEQLYFPRIIENYVLSAE"
     gene            complement(3008..4003)
                     /gene="tsf"
                     /locus_tag="PLI69_04080"
     CDS             complement(3008..4003)
                     /gene="tsf"
                     /locus_tag="PLI69_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOG41701.1"
                     /translation="MEIKAADVAKLRKMTGAGMMDCKNALVEAEGDFTRAQEIIREKG
                     KLVAAKRADRETSEGAVKIRVKGGKGIIVCLGCETDFVSQNSEFQDLVNSIADTAIAG
                     FPADIEALKGCKLPNGDTVEEAISQQTGKSGEKHSLVAYHRLDAPYVAQYVHTINGKL
                     GAIVAFNKPVPEQLGKGVAMQVVSMKPVSISAADCPEEVIAEERKVAIEKTKEEQIRK
                     AVDAALKKAGINPAHCDTDDHIDSNTAKGWLTPEQAKQAREIIATVSAEKASNLPEQM
                     IQSIANGRVQKFLKENTLEEQEYQMGDGKTSVKDTIAAADKDAKVIAFYRVSLND"
     gene            complement(4028..>4542)
                     /gene="rpsB"
                     /locus_tag="PLI69_04085"
     CDS             complement(4028..>4542)
                     /gene="rpsB"
                     /locus_tag="PLI69_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOG41702.1"
                     /translation="NIDKMMEDGTFDTLAKRERLQVTRQKAKLEKNLGSIKDLTRLPS
                     AIFVVDVQKEVNAVREANRLNIPVIAMVDTCCDPTNIDYVIPANDDAAKSIALVTEVL
                     CKAIAEGSEERKLEKEKEKDSQPVAESTEVIAGKRLRQRRERSARAEAVAEESAEETT
                     EETTEETTNE"
BASE COUNT         1180 a         1070 c          977 g         1315 t
ORIGIN      
        1 acttccgtcg ctctccagtt aggtgaatac ctgtcactca actccatgaa atcagaggcc
       61 ggccttatgg ttaccgacaa cccgaaggaa acactctatt atgaaagcct ttcaggcata
      121 aaaccgcata taagtgggag cgcactacat tcgctaatgc aaaattgggc aaaagaaaat
      181 agcattgatc ttggaaatct tgtaattgcc aaagctatac tggagtttcc ttttgaatac
      241 tcaggaaatt acaaagccct cgacaactac cccggcaacc tctttccctg tagaagagta
      301 cgcaccaaaa agcatactct ctacacgcct atagatgaga tttatgatag ctatatgagt
      361 aacggttctc tcaaccgctc attgctgagc tataaaccgg acattggctt atatcttcag
      421 gacctgatta aaaaagagag ttccgaattg accgatgaag atgatctatg gattatgcaa
      481 accatatctt acaccaataa cactaccaat gtgaccacgc attatcctga cttcacccac
      541 tataatgtag ccaagttcaa cggaacgggt tgcaaacgta atcccaagct taggatagtc
      601 tatacgcttc tcaagtagag aaatgtagtt gtagatagaa atagaatacg gctccgaact
      661 tcggagccgt tgttgtatca ggtagtaagg ataaatttta tcttgggaaa ggcctaaatc
      721 ccggcgctgc gaagattatg gtggcgttgt gtccgccgaa cccaaaattg ttgctcatag
      781 ccacacgtac attgcgcttc tgagcactat ttagcgtaag attgagagaa taatcgatag
      841 cttcatcttt ctcccggaaa ttaatggtag gagggataat accatccctg attgcgtgta
      901 tgcatgcgag ggcctcaacc gcaccggcag caccaagtaa gtggccggtc atggatttgg
      961 atgaggaaag attgacgtta tagacaacat ccccaaaaac tttcttgatg gcatccaatt
     1021 ccgctatgtc gccaaggggt gtggatgtac catgcgtatt tatgtaatca acttgatccg
     1081 gagagatacc ggcatcctcg agagcgatgc gcattgactc tgacgctcct tctcctcccg
     1141 gacttggggc agtaatatgg taggcatccg cattctgtcc atacccaatg atctcagcat
     1201 agatttttgc tcctctctta agtgcatgct catactcttc aagtactatt atagctgctc
     1261 cctctccaat cacaaaacca tctctggtag catcaaaagg gcgggaggct gtctgatagt
     1321 catcattgtt ggtagagagt gcgcgcatcg agttgaatcc accgatcgca gtgacagtaa
     1381 tcggagcctc tgaaccaccg gttatcatta tgtccgcctt gcctagttgt atcatggaag
     1441 ctgcaacgca aatagcgttg gcagaactgg tacaagccga ggtggtggag aaattaggtc
     1501 ccctgaaacc atattttatg gagatagaac ctgaggcaat atttgtgata atcttaggta
     1561 taagaaatgg actaaaccga gggggctgcc cttcggtgta gtccattgtt tcctgcgtga
     1621 gagtctcaat gccacctatg cccgagccaa tgataactcc tgttctaccc aggttctctt
     1681 cgttgagggg gatcctgcta tcattaatgg cttgatcggc cgcaatgaga gcgtattggg
     1741 tgtatctatc gtgcttccgc gcctccttta tatccatacc gaatcgttcc ggagagtagt
     1801 cgcgaatctc acacgcaaat tttgttttga aaagagaggt atcgaaacgg gttatgagtt
     1861 ttgacccact aactccaagg tccagatttg aaaaataatc ctccgggttg tctcctattg
     1921 ggttaatggt accgataccg gttatgacaa ctctcttgac taacatcggt ccttatagct
     1981 taaacgctat ttctgattat tctcaatata gtcgatagcg tcttgtacag tgctgatctt
     2041 ctctgccacc tcatcgggaa ttgagatgcc aaatgccttc tcaaactcca ttatgagttc
     2101 aactgtatca agcgaatcag caccaagatc gttggtgaag ctggcgtcag gctgaacttc
     2161 gttctcctca acaccaagtt tgtaaacgat gatagatttt acttttgcgg taatatcttc
     2221 cataataaaa attggtttag ttagtaattt tagttcacac cttgcatcgc aaggaaatgc
     2281 aaaaatagta tattttttta agccttccaa attttatacc tttgcagcaa ggagttcaaa
     2341 ttaatacaaa aatgaccaat ctcgccatat atgcctcagg ctccggcacc aatgccgcga
     2401 acataattcg ctatttcaaa aatcaccctg aaataagcgt caaagtagtt ttatgcaaca
     2461 atccggaggc atttgtcata gaacgtgcaa agagacttga cataccctgc ctagtttttc
     2521 ctaaaagtgg acttgcagag actattaata ttctaaaaga atatgaaatt gactacataa
     2581 tactggcagg ttttctacta aaagtgccaa aaaacattct ggaagcctat cgagatcgca
     2641 taataaatat acatccggcc cttatcccct cacaatacgg aggtaaaggg atgttcggta
     2701 tgcatgtcca taaagctgtt atcgctgccg gtgagaagga atcgggcata actattcacc
     2761 tagtagatga aatatacgat cacggagaga atatttttca gatccgatgt cctgtcaatg
     2821 tagacgacac tccgaaagac cttgccgata ggatccactg tctggagcag ctctattttc
     2881 ccagaataat cgagaattat gtattgagtg ctgagtgatg tgcaaattcg cgcgaattga
     2941 tacgaattta cacaaataaa ggccgacgat gtcagccttt atttgcataa tatctgtatc
     3001 gacttaatta gtcgttgagg gatactctat aaaatgcgat aacctttgca tctttgtcag
     3061 cggcggcgat ggtatccttt accgaagtct taccgtcgcc catctggtac tcctgttctt
     3121 cgagagtgtt ctctttgagg aatttctgta cgcggccgtt ggcgatgctc tgaatcatct
     3181 gctcgggaag gttggatgct ttctctgcag agaccgttgc gattatctct cgtgcctgtt
     3241 ttgcctgctc aggagtgagc cacccctttg cggtatttga gtcaatatga tcatcagtgt
     3301 cgcaatgtgc gggattgatg cctgccttct tgagagctgc atccactgcc tttcttattt
     3361 gctcttcttt tgtcttctca atagccacct tacgctcctc agcaatcacc tcttcgggac
     3421 agtctgctgc agagattgac acgggcttca tcgataccac ctgcatagct acacccttac
     3481 cgagttgctc aggaactggt ttgttgaatg caacgatagc tccgagcttg ccgttgatgg
     3541 tgtgcacata ttgtgcgaca tagggagcat caagtctgtg gtatgccacc aggctgtgtt
     3601 tttcaccact cttgcctgtc tgctgggata tagcttcctc aacggtatcg ccattaggga
     3661 gcttacagcc cttcagggcc tcaatatctg cggggaaacc ggcaatagcc gtatcggcga
     3721 tgctatttac cagatcctga aattctgagt tctgggaaac gaagtccgtc tcacaaccga
     3781 gacatacaat tatacccttg cctcctttta cacgtatttt tactgcaccc tcggaagtct
     3841 cgcggtctgc acgttttgct gccacgagct tgcctttttc acggatgatc tcctgtgccc
     3901 ttgtaaaatc gccttctgct tcaaccaaag cattcttgca atccatcata ccggcacctg
     3961 tcattttgcg aagtttcgca acatctgctg ctttaatttc cattttgtga aaaattataa
     4021 ggttatatta ttcgtttgtg gtctcttcag tagtctcttc agtggtttct tctgcgctct
     4081 cctctgcaac tgcttcagct cttgcagatc tctctcggcg ctgacgaagt ctcttgcctg
     4141 caataacttc tgtgctctca gctacaggct gggaatcctt ttctttctct ttttcgagct
     4201 tacgctcttc cgaaccctcg gcgattgcct tgcaaagtac ctctgtcacg agggcgatgg
     4261 atttggcggc atcgtcattg gcaggaatca cataatcgat attagtaggg tcgcagcaag
     4321 tatcaaccat tgcaatcaca ggaatgttga gacggttggc ctctcttact gcgtttactt
     4381 ccttctgaac gtcaaccacg aagattgcag aaggaaggcg tgttagatcc ttgatggaac
     4441 cgaggttttt ctcaagttta gccttctggc gtgtaacctg gagtctctca cgttttgcca
     4501 atgtatcgaa ggtgccatct tccatcatct tatcgatgtt tt
//
LOCUS       DAOSIE010000103         4443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_17335_length_4443_cov_3.339790, whole genome shotgun sequence.
ACCESSION   DAOSIE010000103 DAOSIE010000000
VERSION     DAOSIE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17335_length_4443_cov_3.339790"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PLI69_04090"
     CDS             <1..1152
                     /locus_tag="PLI69_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG41703.1"
                     /translation="YAMMARGAKIATVPQGKTAMETLRNIPMSIAAVKPNIMLSVPAL
                     AKNFKKNIESSVEKSGKKKIFDFALNLAIWYNQEGWNRGRGLRIFAYPLVKLFDKLIF
                     SKVRMAMGGELNFFIGGGALLDIDIQKFYYALGIPMFQGYGLSEATPVISTNTPKRHR
                     LGSSGILVQPMDLKICDENGNEVPNGVTGEIVIRGENVMAGYWKNPTATADTVKDGWL
                     FTGDMGYMHKSGFLYVLGRFKSLLISSDGEKYAPEGIEESIIVNSKIIQNIMLHNNQN
                     PYTICVVQVDKKALPEGEAGIMAIKAEFDKYRSGGQFAGMFPERWLPTTFIIADEPFS
                     EANKMINSTMKLVRNAVEKAYADRIDFAFTAEGKNPVNEANLAALAKID"
     gene            1170..2888
                     /locus_tag="PLI69_04095"
     CDS             1170..2888
                     /locus_tag="PLI69_04095"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOG41704.1"
                     /translation="MRKYRLFILIAAAVLSALAAYGVFTAPRVEPSDSDRFSAERVAA
                     DIKVISQKPHSVIHPVERAEVRDYLYNRLVDLTDSSVVLYPYENAVEYEGTPIDITNI
                     LAQVDPVIDDPDHPNPTYLMLIAHYDSRYPQKVLDQTVLSYGAADDGYGCGVALESLS
                     QALRYRGEWRQGIKILFTDSEEWKLAGINSALEHNPEIFDNVGLVINLESRGNKGPVL
                     LFETGKNNSKTLALYKKAKAPYTYTFTTVVYEFMPNFTDFTPLKDSYPGLNFSNLDDI
                     NHYHTDLDNYDNISLHTIQHYGAQLEPIIEEYLTNHEYRDPNALVSDRSSIALTIPAL
                     GFFSFSKGGYLLVNSIILALFCLIFSFAVISGRIRPLKVLVASAKILLWALTALIIGE
                     LIAWVISMIVGAKFSIMGVVRGVQFDEWVMMGTAAATALIVTICFLWGRKRSADRISS
                     TAIRKSASTSGGTKYSYNLLFGAMLLMLLLSAVLLLTIGENFFFALPLGLAAASLFLW
                     RITTWRGWLLPAIVVILLHAFSFLYVVITALTMGALGVLPLFIVIYLALLLPLADIYT
                     RKEKNI"
     gene            2895..4016
                     /locus_tag="PLI69_04100"
     CDS             2895..4016
                     /locus_tag="PLI69_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG41705.1"
                     /translation="MFDNIRPYNDRETVEALARVAAHPLTGSISKYFYPDKDRGYLGE
                     LLRSVTGTYDFQLKVMYHVIGAVMKGTTDGVTFSGLEYFETDKKRNFLLLSNHRDIVL
                     DSAFIQFILEDHHLPLTEIAVGDNLIADSYIEDIIRSNRMIKVVRSENPREVYTSSQI
                     FSQYIRSSNSNIWLAHRNGRAKDGVDTTEQGLLKMLDMSGTGDFISNFAELNVMPVSI
                     SYEIEPCGLEKAMETWIRNSTGTYKKAPNEDTESILKGIKQPKGAVHISFCEPITIEE
                     LRETDSFSKNEKFQYLASIIDRKILEGYKLYPSNYIAADILSDEERFSGNYTSDEKNA
                     FNAYLEREISGIPESADRQAVMDNLLRIYANPVFRREQY"
     gene            4051..>4443
                     /locus_tag="PLI69_04105"
     CDS             4051..>4443
                     /locus_tag="PLI69_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41706.1"
                     /translation="MKKILTFLLCASLFLTGCGNTSKMSKSAIMGDGSGVGAEIGSPG
                     GKDTKKTAKGRDTKKSTGSKDTKKTSGSKDTRSASAGTVRSAGPSIGDIMENKAADMA
                     GINGVKVETVKDSKGYEAIKLTFDSGILF"
BASE COUNT         1263 a          927 c         1041 g         1212 t
ORIGIN      
        1 tacgccatga tggccagagg agccaaaata gccaccgtac cacagggtaa aacagcaatg
       61 gagacccttc gcaacattcc gatgagcata gctgcggtta agcccaacat aatgctttcc
      121 gttccggcgc ttgcaaagaa cttcaaaaag aacattgaga gttcggtaga gaagagcggc
      181 aagaagaaaa tattcgattt tgcgctcaac ctcgccatct ggtacaatca ggagggctgg
      241 aataggggca gagggctaag aattttcgcc tacccgcttg taaagctctt cgataaactg
      301 atattcagta aagtgcgtat ggctatgggt ggcgaactca atttctttat tggtggaggg
      361 gcacttctgg atatagatat acagaaattt tattatgcgc ttggcatacc catgttccag
      421 ggatacggtc tttccgaggc tactcccgta atttcaacca atactccaaa gagacaccgt
      481 ctcggctctt ccggcatcct tgtacagcct atggacctga agatttgtga tgagaatgga
      541 aatgaagttc cgaatggagt taccggtgaa attgttatac ggggtgaaaa tgtaatggcc
      601 ggttactgga agaatcctac ggctactgct gatacagtca aagatggatg gctatttaca
      661 ggagatatgg gctatatgca caagtcggga ttcctatatg tgcttggtcg cttcaaatcc
      721 cttcttatct caagtgatgg tgaaaaatat gcacccgaag gtatcgagga gtctataatc
      781 gtgaattcca agattattca aaatatcatg ctccacaaca accagaatcc ctacactatc
      841 tgtgtcgtac aggtggataa gaaggccctg ccggaaggcg aggcgggtat tatggcgatc
      901 aaggcggagt ttgacaaata tcgttcagga ggtcagtttg ccggaatgtt ccctgaaagg
      961 tggcttccca ctacatttat cattgccgat gaacctttca gtgaggcaaa caagatgata
     1021 aacagtacca tgaagcttgt aagaaatgca gtcgagaagg cttatgcgga tagaattgac
     1081 tttgctttta cagcagaagg taaaaatccc gtaaacgaag ccaatcttgc tgctcttgca
     1141 aagattgact aaaacaatac gaaacattta tgagaaaata taggttattt atcctgatag
     1201 ccgctgcggt actctctgca ttggctgcgt acggtgtatt tactgctcca agggtcgaac
     1261 cttcggattc cgaccgtttt tcagctgaaa gagtggctgc cgatattaaa gttatcagtc
     1321 aaaaacccca ttccgtcata catccggttg aaagagctga agtaagagac tatttgtaca
     1381 atcgtttagt tgatctcaca gactcttccg tggtgctcta tccttatgaa aacgctgtgg
     1441 agtatgaggg tacaccgatt gatattacca atatcctcgc acaagtggat cctgtcattg
     1501 atgatcccga tcacccgaat cccacctatt tgatgctgat agctcattat gattcaagat
     1561 acccccagaa ggtactggat cagacagtgc tctcgtacgg tgcagctgat gacggatatg
     1621 gatgtggtgt ggctctggag tctcttagtc aggctttacg ctatcgcgga gaatggagac
     1681 agggcattaa gatccttttc acagactcgg aggagtggaa actcgcaggt atcaattccg
     1741 ctctggaaca caatcctgag atttttgaca atgtagggct tgtaatcaac cttgaaagtc
     1801 gtggcaacaa ggggcccgta ctgctctttg agacgggtaa aaacaattct aaaacccttg
     1861 ctctctataa aaaggcaaag gccccataca cctacacatt tactacggtg gtttatgagt
     1921 tcatgcccaa ttttaccgac ttcacccctt tgaaggattc atatccgggc ctcaatttct
     1981 ccaatcttga cgatatcaac cactaccaca cggatctgga caattacgat aatatatcat
     2041 tacatacgat acagcattat ggtgcccaat tggagccaat catagaggaa tatctgacca
     2101 atcatgaata tcgtgacccc aacgcactgg tatcggacag atcgtctata gctttaacaa
     2161 ttcctgctct cggctttttc tccttctcca aaggcggtta ccttctggtt aactccatta
     2221 ttctggcact tttctgcctt attttcagtt ttgcggttat aagtggcaga ataagaccgc
     2281 tcaaggttct cgtagcttct gcaaaaatac tgttgtgggc tcttacagcc ttgatcatcg
     2341 gtgagctgat tgcgtgggtg atctccatga tcgtaggagc aaagttttcg attatgggtg
     2401 ttgtgagggg agtacagttt gatgaatggg ttatgatggg aactgcggct gctactgcac
     2461 tgatagttac aatctgcttc ctttggggca ggaagaggtc tgctgaccgc atttcttcta
     2521 ccgctattcg taagagtgcc tccaccagtg ggggcaccaa gtattcctac aaccttctat
     2581 ttggggcgat gttgctgatg ctgctcttat cggcagtgct attgttgacc ataggggaaa
     2641 atttcttctt tgcattacca ttggggcttg ctgctgcctc tctcttcctt tggaggatta
     2701 caacctggcg cggatggctc ctgcctgcga tagtagtaat tttattacat gcattctcat
     2761 tcctctacgt ggtcataaca gccctgacga tgggtgcttt aggagtactt ccgctattta
     2821 tagttatcta tctggccctg ctgttgcctc ttgcagacat ttacacccgc aaagaaaaaa
     2881 acatctgata aatagtgttc gacaatataa ggccatataa tgatcgtgaa acagttgaag
     2941 cacttgctag ggtggcagcc catcctttga ccggaagcat ttccaaatat ttctatcccg
     3001 ataaggatag gggatacctt ggagagcttc taaggagtgt taccggtacc tatgattttc
     3061 aattaaaggt tatgtatcac gtcataggcg ctgtaatgaa gggtactaca gacggggtta
     3121 cattctcagg actggagtat ttcgaaacgg ataagaagcg taacttcttg cttttatcca
     3181 atcatcgtga catagtactg gattctgcat tcatacaatt tattctggag gatcatcatc
     3241 tgcccttgac ggaaattgcg gtaggggata atcttatagc agatagctat attgaagata
     3301 taattcgttc caatcgtatg attaaagtgg tcaggagcga aaacccacgc gaagtttaca
     3361 ccagttcgca gatattctct caatatataa gaagcagtaa ttccaatatc tggctggcac
     3421 atcgtaacgg tcgggcgaaa gatggtgtgg atactaccga gcaagggttg cttaaaatgt
     3481 tggatatgag tggtacaggg gactttatct caaactttgc cgagcttaat gtaatgccgg
     3541 tttccatatc gtacgaaata gagccttgcg gcttggaaaa ggcaatggag acctggatca
     3601 gaaacagtac cggcacctat aaaaaggccc cgaatgaaga tactgagagc atactcaagg
     3661 gtataaaaca gcctaaaggc gccgttcaca tctcgttctg tgaaccgatt accattgaag
     3721 aactgcggga aacagattca ttttcaaaaa atgaaaaatt tcagtatctt gcatcaatta
     3781 ttgacagaaa aatacttgaa ggatataagc tttatccatc caattatatt gccgcagaca
     3841 ttctcagcga cgaagaaagg ttttcgggca attatacgtc agatgagaaa aatgctttta
     3901 atgcctacct ggagagggaa atatcaggga ttccggaatc tgccgaccgt caggccgtga
     3961 tggataatct cctgcgtatt tatgccaatc ctgtttttag gagagaacaa tattgaaata
     4021 atattttatt aactataatg ctttaacaac atgaaaaaga ttcttacttt tttactttgc
     4081 gcatctcttt tcttaacggg atgtggcaat acgtccaaga tgtccaagag tgcaataatg
     4141 ggcgacggta gtggggtggg agctgagata ggatcgccag ggggcaagga taccaagaaa
     4201 actgctaaag gcagggatac caaaaagagt accggaagca aggacaccaa gaaaacatcc
     4261 ggaagcaagg ataccaggag tgcatccgcc ggtacggttc gttcagccgg accttctatt
     4321 ggcgatataa tggaaaataa ggcagcagat atggccggga tcaatggagt gaaggtggaa
     4381 accgtaaaag attccaaggg atatgaggcc attaaattga ctttcgatag cggtattctc
     4441 ttt
//
LOCUS       DAOSIE010000104         4374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_17699_length_4374_cov_3.498263, whole genome shotgun sequence.
ACCESSION   DAOSIE010000104 DAOSIE010000000
VERSION     DAOSIE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17699_length_4374_cov_3.498263"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLI69_04110"
     CDS             complement(<1..319)
                     /locus_tag="PLI69_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41707.1"
                     /translation="MPQTSSNQRISFATSKALLDYPDFLEVQLKSFRDFFQLDTTADS
                     RRDEGLFKVFQEIFPIEDTRNNFTLEFIDYFVDPSRYSIEECLEMGLTYKVPLKAKLK
                     LSCN"
     gene            complement(426..803)
                     /gene="rplL"
                     /locus_tag="PLI69_04115"
     CDS             complement(426..803)
                     /gene="rplL"
                     /locus_tag="PLI69_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOG41708.1"
                     /translation="MADIKALAEELVNLTVKEVQELANILKEEYGIEPAAAAVVVAAS
                     EGSAGAAEAEQTEFDVILISAGQAKLQMVKAVKEITGLGLKESKDLVDQAPNAKIKEK
                     VSKAEAEQVKAALEEAGGEVEVK"
     gene            complement(849..1364)
                     /gene="rplJ"
                     /locus_tag="PLI69_04120"
     CDS             complement(849..1364)
                     /gene="rplJ"
                     /locus_tag="PLI69_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOG41709.1"
                     /translation="MRKEEKAQIIETIAAQIVENPNFYLADISGLNAENTSNLRRACF
                     EKEIKLVVVKNTLLQRALEGLDAEGTGINNEEIKTLYPTLKGSTAIMFTSVPSTPAKI
                     IKEFGARLGKPELKSAYLQECAYVGANHLATLVNIKSHDELIGEIIGLLQSPIRRIVS
                     ALQEAKKEVTE"
     gene            complement(1393..2079)
                     /gene="rplA"
                     /locus_tag="PLI69_04125"
     CDS             complement(1393..2079)
                     /gene="rplA"
                     /locus_tag="PLI69_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOG41710.1"
                     /translation="MSKLTKNQKAAVAKVDSSRHYKLLDACALVKEVSYTKFDASVEL
                     AVRLGVDPRKANQMVRGVVTLPHGTGKVTRVLVLCTPDKEAEAQEAGADFVGLDDYIE
                     KIKGGWTDVDVIICTPSVMAKIGAIGRILGPRGLMPNPKSGTVTMDVGKAVQEVKAGK
                     IDFKVDKQGIIHAAIGKVSFDAQKLAENAVEMLNTIIKLKPQALKGTYVKSIYLSSTM
                     SPGIPVDSKK"
     gene            complement(2092..2538)
                     /gene="rplK"
                     /locus_tag="PLI69_04130"
     CDS             complement(2092..2538)
                     /gene="rplK"
                     /locus_tag="PLI69_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOG41711.1"
                     /translation="MAKEIAGFIKLQIKGGAANPSPPVGPALGSKGVNIMDFCKQFNA
                     RTQDKAGKVLPVVITVYSDKSFSFVVKQPPVAILVKEAAKVQTGSAEPNRKKVAAITW
                     NQIREIAREKMPDLNAFTEKSAMNMVAGTARSMGISVEGEFPADVK"
     gene            complement(2554..3162)
                     /locus_tag="PLI69_04135"
     CDS             complement(2554..3162)
                     /locus_tag="PLI69_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOG41712.1"
                     /translation="MSEITKKWYVVRIIGNKEKIAKEYIEKEMGKGMLKHVTDVLVPT
                     EKYFVIKNGKRVSAERNFFPGYLFVEITFPSLKSDNQDDTNERERFEKEILGIAQDGL
                     RELPNIIGFLTEGKERVINPVRQSEIDRIMGRVDELSDQDGEILVPFVVGEAVKIIDG
                     PFKDFSGNVEEILEERRKLKVMVKIFGRKSVIELNFAQVTKE"
     gene            complement(3167..3355)
                     /gene="secE"
                     /locus_tag="PLI69_04140"
     CDS             complement(3167..3355)
                     /gene="secE"
                     /locus_tag="PLI69_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOG41713.1"
                     /translation="MSKLGTYVKESYRELTTKVAWPTWSKLQNSAILVMVSTLILSVV
                     IWLIDFVIKTIMTGLYQL"
     gene            complement(3439..3509)
                     /locus_tag="PLI69_04145"
     tRNA            complement(3439..3509)
                     /locus_tag="PLI69_04145"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3475..3477),aa:Trp,seq:cca)
     gene            complement(3566..>4374)
                     /locus_tag="PLI69_04150"
     CDS             complement(3566..>4374)
                     /locus_tag="PLI69_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOG41714.1"
                     /translation="VPRIVVFLNKVDIVDDPEMIELVEMEVRELLSFYEFDGDNAPVI
                     RGSALGALNGDPQWEEKVVELMEAVDTYIPIPKRDNEKPFLLPVEDIFSITGRGTVAT
                     GRIETGVVKTGDEVQIIGLGEQPRKSVVTGVEMFRKILNQGEAGDNVGLLLRGIDKKE
                     IKRGQVIAHPNTITPHRKFEAAIYVLKKDEGGRHTPFHNKYRPQFFIRTLDITGEIEL
                     PAGVEMVMPGDNVKINVELIYPVALNEGLRFAIREGGRTVGSGQVTKIIS"
BASE COUNT         1153 a         1028 c          896 g         1297 t
ORIGIN      
        1 cattgcagga tagtttgagt ttggctttca agggaacctt atatgtcaaa cccatttcca
       61 gacactcttc gatggagtaa cgtgaaggat ccacgaagta gtctataaac tccagagtga
      121 aattgttgcg cgtatcttcg ataggaaata tctcctgaaa cactttgaaa agaccctcat
      181 cgcggcgact atcagcagta gtatccagct gaaagaaatc acggaaagat ttcaactgaa
      241 cctcgaggaa atcgggatag tctagaaggg ccttggatgt cgcgaatgaa atacgctgat
      301 tactagatgt ttgcggcatt attgaatggt tgttgaaaaa aatcttaaaa aacgacaaaa
      361 aggtttagag ccctcattgg ctctaaaccc ggatttctta gtccctatat cgggagggga
      421 gtgtgttatt taacttcaac ttctccgcct gcctcttcaa gagcagcttt aacctgctca
      481 gcttctgcct tggacacttt ctccttgatc ttggcgttag gtgcctgatc tacgagatct
      541 ttagactctt tcagaccgag accggtaatc tccttaacag cttttaccat ctggagtttt
      601 gcctgaccgg ctgatatgag gattacgtca aattcagtct gctcagcttc tgctgcgccg
      661 gcactgcctt cgctggctgc taccacaact gcagcagctg caggttcgat tccgtactct
      721 tctttaagta tatttgcaag ttcttgaacc tcttttacgg taagattaac cagttcttcc
      781 gcgagagctt tgatgtctgc catgattgtt tattgatttt aattatatta ttattactat
      841 ttaaactgct attcggtaac ctctttctta gcctcctgca gagcggaaac aatccgtctg
      901 atgggcgact ggagaagacc gattatctca ccgataagtt catcgtgaga cttgatattt
      961 acaagagtag cgagatgatt tgcaccaacg taggcacact cctgaagata cgcactcttg
     1021 agctccggtt taccgagtct ggctccgaat tccttaataa tcttggccgg agtactaggt
     1081 actgaggtaa acatgatggc tgtggagcct tttaaagtag gatatagagt cttgatttct
     1141 tcgttgttga taccggttcc ttcagcgtcg agaccttcaa gagccctctg gaggagggta
     1201 tttttcacaa cgacaagttt aatctctttc tcaaagcaag cacgacggag attcgatgta
     1261 ttttctgcat tgagacctga aatgtctgca aggtagaaat tgggattctc tactatctgt
     1321 gctgctatgg tttcaataat ctgagctttt tcttcttttc tcataacatt cgaatatttt
     1381 attcaacagc acttatttct tgctatctac aggaataccg ggactcatag tagaagagag
     1441 gtaaatactc ttcacatatg ttcctttgag agcctgcggt ttgagtttaa tgatagtgtt
     1501 gagcatctcc acggcattct cagcaagttt ctgtgcgtca aatgagacct tgccgatggc
     1561 tgcgtggata ataccctgtt tgtcaacttt gaaatcaatc ttacctgctt tcacttcctg
     1621 aactgcttta ccgacatcca ttgtaactgt accggactta gggttgggca tcaagccacg
     1681 aggacccaga atacgtccga tagcaccaat ctttgccata acggaaggag tacaaataat
     1741 aacatctacg tcagtccaac cacccttgat cttttcaata taatcatcaa gaccaacaaa
     1801 gtcggcacct gcttcctgag cttcggcctc tttgtccgga gtacatagaa ctaatacgcg
     1861 agttacctta cctgtaccat gaggaagagt cacaacgcca cgtaccatct ggttcgcctt
     1921 acgaggatca acacccaaac ggactgccaa ctcaactgaa gcgtcaaact tggtgtagga
     1981 aacctccttt accaaggcac acgcatcgag gagtttatag tgcctgcttg agtcaacttt
     2041 ggcaactgct gccttctgat tttttgtcaa tttactcata gcgtggtgat tttattttac
     2101 atcggcagga aattcacctt caacactgat acccatactg cgagcagtac cggctaccat
     2161 attcattgct gacttctcgg tgaatgcatt aagatccggc attttttccc tggcaatctc
     2221 acgtatctga ttccaggtga tggcagcaac ctttttccta ttgggttcgg cagatccggt
     2281 ctgtactttt gctgcctctt ttacgaggat agctacaggg ggctgcttta caacgaaaga
     2341 aaaagacttg tcgctgtata cggtaattac cacaggtaac accttgccgg ctttgtcttg
     2401 agtgcgggca ttaaattgct tgcagaagtc catgatgttc acacccttgg aaccgagggc
     2461 cggtcctacc ggaggtgaag gattggcagc tccgcctttg atctgcaatt taataaaccc
     2521 ggcaatttct ttagccataa tgtcttttgt ttattattct tttgttacct gagcaaaatt
     2581 caactcaatg acagactttc ttccgaaaat tttaaccata accttgagtt ttcttctctc
     2641 ctcgagaatt tcctcaacat taccgctgaa atctttgaaa gggccgtcga taatctttac
     2701 agcttcccct acaacaaatg gtaccaagat ctctccgtcc tgatcactca attcgtccac
     2761 acgtcccata atacggtcaa tctccgactg acgtaccgga ttgattaccc tttccttgcc
     2821 ttcagtaagg aaaccgataa tatttggaag ttcgcgaagt ccatcctggg caatacctag
     2881 gatttccttc tcaaacctct ctctttcgtt tgtgtcatct tgattgtcgg acttaagaga
     2941 ggggaatgta atttctacaa agagataacc gggaaaaaaa ttacgctctg cgcttacgcg
     3001 cttgccattt tttatcacaa aatacttttc agtaggaacc aggacatcag taacatgctt
     3061 aagcatgcct ttgcccatct ccttttcaat atattctttg gcgattttct ctttgttgcc
     3121 aattatacgg acaacatacc acttcttggt gatttcactc ataacattac aattggtaaa
     3181 gacctgtcat aatggtttta atgacgaaat caataagcca gattacaact gacagaatca
     3241 gcgtgctgac cataaccagt atggctgaat tctggagctt gctccaggtc ggccacgcaa
     3301 cttttgttgt aagttctctg taagactctt ttacgtaagt tcctaatttg ctcattgtta
     3361 ttattttgtt ggcaaaaaca attggaagcg gttgttttgc cggttaataa cataccaacg
     3421 cgtaaccgtc ggtatttgca ggggaagaga gattcgaact cccatcgatg gttttggaga
     3481 ccactattct acccttgaac tattccccta tgataacaag agggtggcgc taatatggtc
     3541 accctcctga tattcgtatt tataattaac tgataatctt agttacctga cctgaaccta
     3601 ccgtacggcc accttcacgg attgcgaaac gtagaccttc gttcagagct acaggataga
     3661 taagctcaac attaatctta acgttgtcac cgggcattac catctcaaca cctgcaggga
     3721 gttcaatctc acctgtaatg tcaagtgtac ggatgaagaa ctgaggacga tatttgttgt
     3781 ggaaaggagt gtgacgacca ccttcatctt tctttaatac ataaattgct gcctcaaatt
     3841 ttctgtgagg tgtaatggtg ttggggtgag caataacctg acctctcttg atctcttttt
     3901 tgtcgatacc acgaaggagg agacctacgt tgtcaccggc ctcaccctgg ttgaggatct
     3961 tacggaacat ctcaacacct gtaacaacgg attttctggg ctgttcgcca agaccaatga
     4021 tttgaacctc gtcacctgtc tttacaacac ctgtctcaat acggccggta gcaactgtac
     4081 cacgaccggt aattgagaag atatcctcca caggcaatag gaaaggcttc tcgttatcac
     4141 gcttggggat aggaatatag gtatcaacgg cctccataag ctcaactacc ttttcctccc
     4201 actgaggatc accgttgaga gcaccgagag cggaaccgcg aataacggga gcgttgtcac
     4261 cgtcaaactc gtagaagctc aagagttcac gaacctccat ctcaacgagc tctatcattt
     4321 caggatcgtc aacaatgtcc accttgttta agaaaacaac gatacgggga acgt
//
LOCUS       DAOSIE010000105         4351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_17824_length_4351_cov_3.574255, whole genome shotgun sequence.
ACCESSION   DAOSIE010000105 DAOSIE010000000
VERSION     DAOSIE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17824_length_4351_cov_3.574255"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLI69_04155"
     CDS             complement(<1..1171)
                     /locus_tag="PLI69_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG41715.1"
                     /translation="MKKLFALCMICTLIFTAGCTQVKVDSSMPYSVENGRIVLETPKR
                     PAGQQSALAMTAEPLDTVRVGFVGLGMRGSDAVIRYTYIDGGCITALCDLLPERTEAS
                     KNALLERGWPEPALYSGEEGWRELCENPDVDLVYICTPWQNHAEISVYAMQCGKHVAV
                     EVPGAMTIAECWDIINTSESTRKHCMMLENCCYDFFEMTALNMAQQGLFGEILHVEGA
                     YIHNLDPYWKLYQGNWRLDYNQKHRGDNYPTHGLGPACQVLNIHRGDRMEYLVSMDTK
                     SVHGKEVSKEMMGTEEFANGDHTVTLIRTVGGKQIEIQHNVYTRRPYNRMYQITGTEG
                     FANKYPVRGFALMSHNLPEGREEYAGLDGERFVSPELLKILLEEYKHPITAEIEEK"
     gene            complement(1196..1660)
                     /locus_tag="PLI69_04160"
     CDS             complement(1196..1660)
                     /locus_tag="PLI69_04160"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG41716.1"
                     /translation="MDIIKVRVARPRDRKSLTDFQVQLALETEGQELDRRIVSQGVKS
                     VLDDPQKATYFVAELEGVRGIVGCLMITDEWSDWRNGWVWWIQSLYVLPEYRNRGIFT
                     ALFEHVWMIVDSTSDVKGIRLYVDKRNISAREVYEAIGMTGEHYTTYELMKE"
     gene            1761..2867
                     /locus_tag="PLI69_04165"
     CDS             1761..2867
                     /locus_tag="PLI69_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG41717.1"
                     /translation="MNKKFLYLLAIAGIALLLSSCNKTSSITGTITDETASAPGAVIV
                     FTDLLTEKADTVQIVDGKFEYSCNANDEGCFSVALVTDFNMRNNNNALFIPEAGKITI
                     NLNEPATVEGGKLNKALTEFVDGITQIRDEAIGKFEEARLLPQEEATVKMQEVQDNYS
                     KAVREFSDKTIDANPNGFLGFYALSNFIYDLSYEDLKAYVEKMGAFTKNHPLVIGQIA
                     AGEAMESTAEGKMFVDFEGKTPAGEAVKLSDYVGQGKYVLVDFWASWCGPCKREVENT
                     LLGVYDKFTPKGLVILGVAVWERNGDNSASVAAMEELGMKWDQIFMGDDKTPTDLYGI
                     SGIPHIILFGPDGTIVKRNLRGEELVKLVDEVLK"
     gene            complement(2860..4038)
                     /locus_tag="PLI69_04170"
     CDS             complement(2860..4038)
                     /locus_tag="PLI69_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOG41718.1"
                     /translation="MNTIFDEIIDRRGTQAIKTDFLKKLYGREDLIPLWVADMDFRTP
                     DFIVEALRGRFNHEIYGYSTPPDSYWDSIIKWERELHGWEFSREELSYIPRIVRGIAF
                     VLQCFTSPGDAVIIQPPVYMPFFHIPRDNGRRLIMNPLIRTEEGYGMDLEGLEEICRN
                     SSPKLLILCSPHNPAGVVWPAEVLGEVAHICSKHGVIVVSDEIHADMALFGNHHTPFA
                     MASEEARNISITFAAPTKTFNIAGLVSSFCVVHNADIRERFFGWLQANHLASPTFIAA
                     VATEAAFTKGAEWRKQMLSYVEENVRFVEEFLSTNIPGIRAVRPQASFLVWLDCRELG
                     VDQAALKDLFINKARLALNDGEAFGKEGSGYMRINVGCPRAVLSRAMVQLSTAVRESI
                     "
     gene            4121..4194
                     /locus_tag="PLI69_04175"
     tRNA            4121..4194
                     /locus_tag="PLI69_04175"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4155..4157,aa:Pro,seq:agg)
BASE COUNT         1189 a         1041 c          972 g         1149 t
ORIGIN      
        1 ctttctcttc gatctcagca gttatcggat gcttgtactc ttccagaagt atcttcaaca
       61 gctccggact gacaaagcgc tctccatcca gtcccgcgta ctcctcgcga ccttcgggaa
      121 gattgtggct cataagagca aaaccacgta ccggatattt atttgcaaag ccctcggtac
      181 ccgtaatctg gtacattctg ttataggggc gacgagtata gacattgtgc tgaatctcta
      241 tctgctttcc gcccaccgtg cgtatcaaag taacggtgtg atcgccgtta gcgaactctt
      301 ccgtacccat catctccttg gaaacctctt tgccgtgtac agatttggta tccattgaga
      361 ccagatactc catcctgtcg cctctatgga tattgagcac ctgacaggcg gggccgaggc
      421 catgggtggg atagttgtcg ccgcgatgct tttgattgta gtcaagcctc cagttgcctt
      481 gatagagttt ccaataggga tcgaggttgt ggatataggc tccttcaaca tgcagaatct
      541 cgccaaacag accctgctga gccatattga gcgcagtcat ctcaaagaaa tcatagcaac
      601 agttttccag catcatgcaa tgcttgcggg tactttcgct ggtattgatg atatcccaac
      661 actcagctat ggtcatagct cctggtacct ctacggctac atgcttaccg cattgcatag
      721 cataaaccga tatctctgca tgattctgcc agggtgtaca gatatagacc agatcgacat
      781 cgggattttc acagagttcg cgccacccct cctctcccga ataaagtgca ggctcgggcc
      841 aacctctctc cagaagtgca tttttggagg cctccgttct ttcgggaagc aaatcacaaa
      901 gtgcggttat acaccctcca tctatgtatg tgtagcgaat aacggcatca gaacctctca
      961 taccgagtcc tacgaagccc accctgacgg tgtcaagcgg ctcggcagtc attgccagag
     1021 cgctctgctg tccggcagga cgtttaggcg tttcaagtac gattctgcca ttttctacgc
     1081 tatatggcat tgaagagtcc actttcacct gcgtgcaacc ggcagtgaag attaatgtgc
     1141 agatcatgca caatgcgaaa agttttttca tattgttttg tgtaaacgat taaaatcact
     1201 ctttcatcaa ctcataagtg gtgtagtgtt cgccggtcat gcctatcgcc tcatagactt
     1261 ccctggcgct gatgttgcgt ttatctacat aaagacgaat tcccttgaca tcggaggtag
     1321 aatcaactat catccaaacg tgttcaaaaa gggcggtaaa gatccctcta ttacgatact
     1381 caggcaaaac atagagggat tgtatccacc aaacccatcc gttgcgccag tcactccact
     1441 catcggtaat cataaggcat cctactatac cccttacacc ctcaagctcc gctacaaagt
     1501 aagtggcctt ttgggggtca tccagaactg acttaacgcc ttgagagact atacgacgat
     1561 ccaactcttg accttcggtc tcaagagcca gctgcacctg aaaatctgtc aaacttttgc
     1621 gatcccgggg tcttgcaacc ctcactttta ttatatccat aagaaatagt ttaatatttc
     1681 acaaaaatat attttttttg cttaactttg tagtttcttg aactttatag ttagatcaaa
     1741 caaacaaaaa aatatcaatt atgaacaaaa aattccttta tttgctggca attgccggca
     1801 tcgcactgct tttaagcagt tgcaacaaaa ccagttcaat aacaggcact attaccgatg
     1861 aaaccgcttc tgctccggga gctgtcatcg tattcacaga cctgctaaca gaaaaggctg
     1921 acaccgtaca gattgttgac ggtaaatttg aatattcttg taatgcaaat gacgagggat
     1981 gttttagcgt tgctcttgta actgacttca acatgaggaa caacaacaat gctttattta
     2041 tacccgaagc cggtaaaatc actatcaatc ttaacgaacc ggccactgtt gagggtggaa
     2101 aattaaacaa agccctaact gagtttgtag atggtattac ccaaattaga gatgaggcca
     2161 taggtaagtt tgaagaagcc cgtctccttc ctcaagaaga agctacggta aaaatgcaag
     2221 aagtgcagga caattatagc aaggcagtac gtgagttttc ggataaaacc atcgatgcca
     2281 atcctaacgg cttcctaggt ttctatgctc ttagcaactt catttacgat ctttcgtatg
     2341 aggacctcaa agcttacgta gaaaaaatgg gggcttttac aaaaaaccac cctctcgtaa
     2401 taggtcaaat agcagcaggc gaggccatgg agagtaccgc agaaggtaaa atgttcgtgg
     2461 attttgaagg taaaactcca gcaggagaag ctgtgaaact ttccgattat gttggtcaag
     2521 gcaaatatgt cctcgtggat ttctgggcat cctggtgtgg tccctgcaag agggaggttg
     2581 aaaacacact tttgggtgtt tatgacaaat tcacaccgaa aggccttgtg attctgggag
     2641 tagctgtatg ggagcgtaat ggagacaact ccgctagcgt agcagctatg gaagaattgg
     2701 gtatgaaatg ggatcagatc tttatgggtg atgacaagac acctaccgat ctatacggca
     2761 tttccggtat ccctcatatc attctcttcg gtcccgacgg tactattgtt aaacgtaacc
     2821 ttcgcggcga agagcttgtt aagcttgtag acgaagttct taaatagact cccgcacagc
     2881 ggtagagagc tgtaccattg cacgtgacaa tactgcacgc ggacagccca cattaatacg
     2941 catataacct gagccctcct tgccgaacgc ctcaccgtcg ttcaacgcaa ggcgggcttt
     3001 gtttatgaag aggtctttga gagcagcttg gtcaactccg agttcgcgac agtcaagcca
     3061 gacaagaaaa gaggcttgcg gacgtactgc gcgtatgcct ggaatattgg ttgagagaaa
     3121 ttcctccaca aagcgcacat tttcctccac gtaagagagc atctgcttac gccattctgc
     3181 tccctttgta aatgccgcct ctgttgcaac agcagcaata aaggttggtg aagcgaggtg
     3241 attagcctgg agccaaccga agaagcgttc gcgtatatct gcattatgca ccacgcagaa
     3301 ggagcttacc aagcctgcta tgttgaatgt tttagtggga gcggcaaaag tgattgaaat
     3361 attgcgcgcc tcctcagatg ccatagcgaa cggagtatga tgattgccaa agagtgccat
     3421 gtcagcgtga atctcatcag agactacaat aactccatgc tttgagcaga tatgtgccac
     3481 ttcacccaat acctccgccg gccacactac ccctgccggg ttgtgcggac tacagagtat
     3541 aagaagcttg ggagaggagt tcctacagat ctcttcgagt ccctctaggt ccattccgta
     3601 gccctcttca gtgcgaatca gaggattcat tataagacgc ctgccattat ctctcggaat
     3661 atggaaaaat ggcatatata ccggaggttg tattattact gcatctcccg gcgaagtaaa
     3721 gcactgcaat acaaaggcga taccacgtac aattcgggga atataggaca actcttcacg
     3781 ggaaaattcc catccatgca actctctttc ccacttgata attgagtccc aataactatc
     3841 gggaggtgtg gaatagccgt aaatctcgtg attgaagcgc cctctgagag cctccacaat
     3901 aaagtcaggt gtcctgaagt ccatgtcggc aacccataac ggtatcagat cctctctacc
     3961 gtacagtttt ttaaggaaat ctgtcttgat agcctgagtg ccgcgtctat cgattatttc
     4021 gtcaaaaatt gtattcatag agcaaagata agaataaaaa aagcagtttc ttttggttat
     4081 ttaagtaaag agagttattt ttgcaacccc aattagggat cgggaagtgg cgcagttggt
     4141 tagcgcacca cgttagggac gtgggggccg tccgttcgag tcggatcttc ccgacaaaaa
     4201 gagagagtga ccttggtcgc tctctttttg ttagtaaata aaaacaatta tccgaatcaa
     4261 atacaaaagc cccggcagct gttgctgtcg gggctttata aagttggcgg ctacctactc
     4321 tcccacttgg tatagcagta ccatcggcgt t
//
LOCUS       DAOSIE010000106         4288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_18151_length_4288_cov_3.538153, whole genome shotgun sequence.
ACCESSION   DAOSIE010000106 DAOSIE010000000
VERSION     DAOSIE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18151_length_4288_cov_3.538153"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLI69_04180"
     CDS             <1..598
                     /locus_tag="PLI69_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOG41719.1"
                     /translation="QDGLKPEHVVVSNASCTTNCLAPIVKVLHDNFGIVEGLMTTVHS
                     VTATQKTVDGPSSKDWRGGRAASANIIPSSTGAAKAVGKVIPEMNGKLTGMSFRVPTV
                     NVSAVDLTCRLAKGCTYDDVKNAMKKASEGELKGILGYTEDKVVSSDFITDPRTSIFD
                     ADAGIQLNANFVKVIAWYDNEWGYSNKILDMIAYTATK"
     gene            complement(690..1787)
                     /locus_tag="PLI69_04185"
     CDS             complement(690..1787)
                     /locus_tag="PLI69_04185"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOG41720.1"
                     /translation="MLKFMNNRWRNILSNFELEGTPVEISRLGNGLINDTYRLRIIEP
                     DLPDYVVQRINSNVFKDVDKMQRNIDRVTAHIRGKMFDRGTPGGPERLLTFVKSSTGK
                     SYYEIDGEFWRISKYIDRSRSREDVTPELAYITGKSFADFQYMLSDLDGEPLEETIPN
                     FHNIEFRIRQLREAVDEDKAGRLERVRPLVEELLDRAEEMSLAERLGREGKLKKRITH
                     CDTKVNNLLFDEDDNVLCVIDLDTTMPGYVLSDYGDFIRTAGNTGTEDDKDLDNVGLD
                     IEIFKAFTKGYLEGAKPFITDIERELLPFGAKLLTYMQTVRFLTDYLNGDTYYKINYP
                     EHNYDRTLAQKRLLDSLDAHEEEMKEFIASL"
     gene            1849..3423
                     /locus_tag="PLI69_04190"
     CDS             1849..3423
                     /locus_tag="PLI69_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOG41721.1"
                     /translation="MRYFLLTTLLLAASVTAFSQVRFDAGFESGNLGKVVLLDSTKVI
                     VSPRDTVEHLSYLIHGKFDPPNPIDTSLAANANWYYFRISGIRGKQIYLTLRDNGVHG
                     LSYSYDGDNWNHFPLNESPRRRLDKRFDKDTVFIALYRPYTYSYLQERIAEWGKRKGT
                     QIDTIGWSFEKRPLQLLHITDPNVPASEKKRLWIHGRQHPSETPGSWLLDAFIEKLTS
                     DTPEARSLRAQIDAYILPFANPDGVFNGLSRSNVIGVNQEINFGRSEDSTVVEVRAMK
                     EMFRKLHDEQPIDLLINNHSQHADCAIYWMHTSSSTSMEYARKQWVFAGLTCAFNPYM
                     YPKEMLFSDVAPRYCEGWFWNNAGERTIALTLESPYTFYSFNKEGEWVNDSNLKYFGE
                     RLLEAVAEYFGLSTPGRLLIENPENPKGWIPLSNQTIAYMGESGWSALAAGSKITYRA
                     DMVEAGEYKLYRYLPGQNVEPPKGRNDFGDVDPGEHGWVFECNYTQKRDGRFKYVYRA
                     KMAGDIADAILLVKKK"
     gene            complement(3434..>4288)
                     /locus_tag="PLI69_04195"
     CDS             complement(3434..>4288)
                     /locus_tag="PLI69_04195"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOG41722.1"
                     /translation="LFSFVIQAVSFHFIHKKGNLLGKNTYKAFLFINGLMGPILIGAA
                     VSTFFFGAEFTINRGNIADMGNPIISQWNTAWHGLEALWGSTLPGGFHLWNVVLGLAI
                     FFLVRSLGALYFINNIRNEEIVSRARKHVLINSIAFLIFFLPYLIRLLLKDGWTGQPD
                     GTIVVEEYKYLKTILANWWMIVILLVGVVGVLYGFILGSFYKKSRKGIWFAGVGTVMV
                     VLVVLLTAGFNGSAYYPSLTDPNSSLTIANSSSSLFTLRTMSVVSLFIPFVVAYISYV
                     WKKMDFHI"
BASE COUNT         1237 a         1045 c          916 g         1090 t
ORIGIN      
        1 tcaggatggc ctcaagcccg agcatgtggt ggtttccaat gcctcctgca ccacaaactg
       61 tttggcaccc atagtaaagg tactccatga caattttggt attgtggagg gtctaatgac
      121 cacagttcac tctgttacgg caacccagaa aacagttgac ggtccctcct caaaggactg
      181 gagaggcggt cgcgcagcaa gtgccaacat aattccttcc agcacaggtg ctgcaaaggc
      241 cgttggtaaa gttatccctg agatgaacgg taagcttacc ggaatgtcat tccgcgtacc
      301 tacggtaaac gtttcagctg tcgatctaac ctgccgcctt gcaaagggct gcacttacga
      361 tgatgttaaa aatgctatga aaaaagcttc ggaaggggaa ctcaagggta ttctcggtta
      421 tactgaggac aaagttgttt caagtgactt tatcaccgat ccccgcacct ccatcttcga
      481 tgcagacgct ggcatccagc tcaacgccaa cttcgtcaag gttatagctt ggtatgacaa
      541 cgagtgggga tattccaata agattctgga catgattgct tacacagcaa ctaagtaata
      601 aaaagagtcc tcacggactc ttttttattg gtgcaggttg caggttgcgg gttaagtacc
      661 acgcacctcg taactcgtac cgcgcaacac tatagcgaag ctatgaactc tttcatctcc
      721 tcttcgtgag catccaggga atccaggagg cgtttttggg ccagagtacg atcgtaatta
      781 tgctcaggat agttgatctt atagtaggtg tcaccgttga gatagtctgt caggaaacgg
      841 actgtctgca tgtaagtcag cagtttggct ccgaatggca acaattccct ttcaatatcg
      901 gtaataaaag gctttgctcc ttctaaataa ccttttgtaa aagccttgaa tatctcaata
      961 tcaagtccca cattgtcaag gtccttatca tcctccgtac ctgtattgcc cgccgtacgg
     1021 ataaaatcac catagtctga gagtacgtag ccgggcattg tagtatcaag gtctataaca
     1081 cataggacat tatcatcctc atcaaataga agattattga ccttggtatc gcaatgagtt
     1141 atcctttttt tcagtttgcc ttcacgtccc aaacgctcag caagtgacat ttcttcagcc
     1201 ctgtcaagca actcctcaac taaaggacgc actcgttcca gacggcctgc tttatcttca
     1261 tctacagcct ctctaagctg cctgattcga aactcgatat tgtgaaaatt gggaattgtc
     1321 tcctcgagag gctcaccatc cagatctgaa agcatatatt gaaagtctgc aaacgatttg
     1381 cctgtgatat atgccaattc cggtgttaca tcttcgcgac tcctggaacg gtcaatgtac
     1441 ttggaaatcc tccagaattc gccatctatc tcataataag atttgcctgt actgctttta
     1501 acaaaagtaa ggagcctctc aggaccaccc ggagtccccc ggtcaaacat ttttccacga
     1561 atatgagccg ttaccctgtc aatattgcgc tgcatcttgt caacatcctt aaagacattg
     1621 ctattgatgc gttgtaccac atagtcaggc agatcaggct cgataattct aagcctgtac
     1681 gtgtcgttta tcagaccgtt acccagacgg gatatctcta ccggcgtgcc ttccagttcg
     1741 aaattcgaaa gtatatttct ccacctgttg ttcataaact tgagcatttg ccttacttgt
     1801 aaaggtagtg attaattgtt atttttgcaa aaaataagcc atactataat gagatatttc
     1861 ttacttacaa cactgcttct ggctgcatca gtaactgcat tctcacaagt ccgatttgac
     1921 gccggtttcg agagcgggaa cctcggaaaa gtggtgcttc tcgactcaac aaaagtgata
     1981 gtctccccaa gagatacggt tgagcacctc tcctacctta tccacggaaa atttgatcct
     2041 cccaacccga ttgatacctc cctggcagca aatgccaact ggtattactt ccggattagc
     2101 ggcataagag gcaaacagat atatcttact ctaagggaca acggagtaca cggactctca
     2161 tattcctacg atggcgacaa ttggaaccat ttccccctaa acgagtcacc ccgcagacgg
     2221 ctggacaaac gatttgacaa agacactgtt tttatcgcac tctaccgtcc ctacacatac
     2281 tcctacctcc aggaacgcat tgcagagtgg ggcaagagaa aaggcactca aattgacact
     2341 ataggatgga gtttcgaaaa gaggcccctg cagctactgc acattaccga tcccaacgta
     2401 cctgcatcag agaaaaaacg cttgtggatc cacggccgtc aacacccaag cgaaactccc
     2461 ggctcgtggc tcctcgatgc tttcatagaa aaacttacct ctgatacgcc ggaggcaaga
     2521 tcacttagag ctcaaataga tgcatacata ctcccttttg ccaacccgga cggagtcttt
     2581 aacggacttt cgagatcaaa tgtaatcgga gtgaatcaag agatcaactt tggcaggagc
     2641 gaagacagca cagtagtgga agtaagggca atgaaagaga tgttccgcaa actacacgat
     2701 gaacaaccta tagacctgtt gatcaacaac cattctcagc atgccgactg tgccatctac
     2761 tggatgcaca ccagcagcag cacctccatg gagtatgcgc gtaaacaatg ggtctttgcg
     2821 ggactcactt gtgcctttaa tccctatatg tatcccaaag agatgttgtt ctccgatgta
     2881 gctcctagat attgcgaggg gtggttctgg aataatgcag gcgagcgcac cattgcattg
     2941 actctggaga gtccatacac cttctactcc ttcaacaagg agggtgaatg ggtaaatgac
     3001 tccaacctca agtactttgg tgagcgcctt ttggaggccg ttgccgagta ctttggatta
     3061 tcaacccccg gccgtctcct tatagagaat cctgagaatc cgaaaggctg gatacccctc
     3121 tccaaccaga ccatcgccta tatgggtgaa agcggttgga gtgcattggc agccggatcc
     3181 aagataactt acagggcaga tatggtggaa gcgggagaat ataaactcta ccgctatctt
     3241 ccgggacaaa acgtagaacc tcctaaaggt cgcaacgatt ttggcgatgt tgatcccggc
     3301 gagcacggat gggtattcga atgcaactat acccaaaaaa gagatggtcg cttcaaatat
     3361 gtatatagag caaaaatggc aggtgatata gccgatgcca ttctgcttgt aaaaaagaaa
     3421 taacctctca aacctaaatg tgaaaatcca ttttcttcca cacgtaagaa atataggcca
     3481 caacaaatgg tataaagagc gacactacac tcatagtgcg tagcgtaaag agactggatg
     3541 atgaattggc tatggttagc gatgaatttg gatcagtcag cgatggataa tatgctgaac
     3601 cgttgaagcc ggctgtcagt aatacgacaa gcacaaccat aaccgtacct acacccgcaa
     3661 accatatccc cttacgactt tttttgtaga aagatcccag gataaagcca tacaataccc
     3721 caactacccc tactaacaaa atcacaatca tccaccaatt tgccaaaatc gtcttcaggt
     3781 atttatactc ctctaccact atggtaccgt caggttgccc ggtccaacca tctttcagaa
     3841 gtaggcgtat cagataaggc aaaaagaata ttaagaaggc tatagagttg atgagcacat
     3901 gtttacgagc tctgctcact atctcttcgt tacggatatt gttaataaaa tacaaagctc
     3961 cgagcgaacg aacaaggaaa aatatagcca aaccgagaac cacgttccag aggtggaagc
     4021 ctcccggcaa tgtgctgccc catagagctt ccaaaccatg ccatgcggta ttccactgcg
     4081 atatgatggg attgcccatg tcggcgatat tgcctctatt tatggtaaat tccgctccaa
     4141 aaaagaaggt agagacagcc gcacctatga gtattggtcc catcaggccg ttaatgaaga
     4201 ggaatgcctt gtaagtattt ttaccaagca aattgccttt tttgtggata aaatgaaaag
     4261 agactgcttg tatgacaaag ctgaagag
//
LOCUS       DAOSIE010000107         4281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_18194_length_4281_cov_4.280170, whole genome shotgun sequence.
ACCESSION   DAOSIE010000107 DAOSIE010000000
VERSION     DAOSIE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18194_length_4281_cov_4.280170"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..600)
                     /gene="rpsI"
                     /locus_tag="PLI69_04200"
     CDS             complement(211..600)
                     /gene="rpsI"
                     /locus_tag="PLI69_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOG41723.1"
                     /translation="MEVINALGRRKSAVARVYLSAGKGNITINGSPIDEYFKEETLKY
                     IVNQPLNLTGTVANFDIKANIDGGGVKGQAEALRLAISRALCKVDEEAYRPVLKSNGF
                     LTRDAREVERKKPGQPGARKRFQFSKR"
     gene            complement(610..1059)
                     /gene="rplM"
                     /locus_tag="PLI69_04205"
     CDS             complement(610..1059)
                     /gene="rplM"
                     /locus_tag="PLI69_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOG41724.1"
                     /translation="MDTQSYKTISANSATVNKEWVVVDATDQVLGRFAAKIAVMLRGK
                     HKTNFTPHVDCGDNVIVLNAGKIRLTGNKMTQKQYVRHTGYPSGQRFTTPKELLERKP
                     LAVLHHAVKGMLPKNRLGAQLLKNLYLYEGNEHPHQAQQPKQINLND"
     gene            complement(1116..2099)
                     /gene="pfkA"
                     /locus_tag="PLI69_04210"
     CDS             complement(1116..2099)
                     /gene="pfkA"
                     /locus_tag="PLI69_04210"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOG41725.1"
                     /translation="MENRIRRIGVLTSGGDAPGMNAAIRAVVRSAIFYHCEVVGIRKG
                     YTGLVERQFMGMQSHSVSKIISQGGTILKSSRCPEFNDPQVRRSAVDNMREAGIDSLV
                     VIGGDGSFRGANDLFKEFDFPVVGIPGTIDNDIYGTDYTIGFDTAINTALEAVDKLRD
                     TADSHNMVFFVEVMGRDAGFIALHTAIGTGAEAVLVPEYPTDIKKLCDYLLNERRKNK
                     TSGIVIVAEGDDAGGAMEIAAQVKRRLPDYETRVTTLGHIQRGGSPSCTDRVRASILG
                     HAAVKALLDGRKGVMVGLINDDIEYTTFDLACSRHNEINKTWYDIARILSI"
     gene            2181..3155
                     /gene="dusB"
                     /locus_tag="PLI69_04215"
     CDS             2181..3155
                     /gene="dusB"
                     /locus_tag="PLI69_04215"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOG41726.1"
                     /translation="MKISTLDLGEKPLLLAPMEDVTDASFRCICKDYGADMVYTEFVP
                     SDGLIRDARKALSKLVIYDYERPIGIQIYGHIPESMVEAAKMAEEAKPEVIDLNFGCP
                     VNKIAKRGAGSGMMREPDKLVEITRRVVEAVKLPVTVKTRLGWDEESKIIVDLAERLQ
                     DVGIAALTIHGRTRSQLYKGEADWTLIGEVKRNPRMRIPIIGNGDITTPEDAAQAFDR
                     YGVDAIMIGRATFGRPWIFKEIRHFLNTGEQLPPISVAEKVELAKKHLAKSIEVKGDR
                     VGLLEMRRHLSSYFKGLDNFKETRLKLVTTNDPAELITVLDFINEKWQ"
     gene            3423..>4281
                     /gene="lpxK"
                     /locus_tag="PLI69_04220"
     CDS             3423..>4281
                     /gene="lpxK"
                     /locus_tag="PLI69_04220"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOG41727.1"
                     /translation="MTLKLRNRLYDKGVFKSQQFDIPVISIGNITAGGTGKTPTTELL
                     VRLLKPHHKVAVLSRGYKRKTRGFRIVDTDSTSREVGDEPLQMRKKLEDVIVAVDTNR
                     KRGITQLLALPQEMRPDVIVLDDAYQHRKVTPLKNILLIDYKRPIFRDNLLPIGSLRD
                     LPEQICRADAIIITKSPEHLDEWERETIKKANRINSDSPCFFARIKYRPIEPIFPELG
                     DKRYIYSKEVGLFTGIANVRPLLLHLSDIYEQITHTSYRDHHEFTRSNIHDIEYFARS
                     NPQALILTME"
BASE COUNT         1197 a          944 c          984 g         1156 t
ORIGIN      
        1 ggacatttta attgaattaa tttttgtagt agttttttcc tgttttactc ccctttaaaa
       61 gcgggagtcc tctttttgtc aatcaggagt agcggttatg acggtctcct agattttccg
      121 cagacggaca aacttcaggc agccatatca taagatgtga gtcctgctgt tttaaatccg
      181 ttctgtgcaa ataaaccagc gtaataactc ttaacgtttg ctgaattgga agcgttttct
      241 tgcgccgggc tgtccgggct tctttctctc aacctcgcgg gcatcgcggg tgaggaagcc
      301 gttggacttc aatacagggc gataagcctc ttcgtcaact ttgcaaagtg cgcgagaaat
      361 cgcaagacgg agagcttctg cctgaccttt gacgccgcca ccatcaatgt tggctttgat
      421 gtcaaagttt gcaactgtac ctgtaagatt gagaggttgg ttaacaatgt acttgagagt
      481 ctcctcttta aagtactcat caatggggct gccgttgatg gttatgttac ctttacctgc
      541 gctgagataa acgcgagcaa ctgctgattt acgtcttcca agggcgttaa ttacttccat
      601 gctctgtttt tagtcgttta aattaatttg ttttggttgt tgtgcctggt gcggatgctc
      661 gttaccttca tataggtaga gatttttcag caactgtgct ccaaggcggt tcttcggcaa
      721 catgcctttc acggcgtgat gcagaacagc taacggctta cgctccaaga gttccttagg
      781 cgtagtgaat ctttgtccac ttggatagcc ggtgtgacgg acatactgct tctgagtcat
      841 cttgttgcca gtaaggcgta ttttgcctgc gttgagcaca ataacgttgt caccacaatc
      901 cacatggggg gtgaaattgg ttttgtgctt tccacgaagc ataaccgcaa ttttggcagc
      961 gaaacggcca agaacctgat ccgtagcatc aaccactacc cactctttgt tgacggtagc
     1021 gctgttagct gagattgtct tgtaactttg tgtgtccact gtgttgatat ttaagtttat
     1081 ataaaatttt ggggctgcaa agttaaacaa atttattaaa ttgacaaaat acgggcaata
     1141 tcataccaag ttttgtttat ctcattgtgg cgcgaacagg ccaaatcgaa agtagtgtat
     1201 tcaatatcat cattaataag gccaaccatt acccctttac gaccatcgag caaggcttta
     1261 acagcagcat gaccgaggat acttgcccgc accctgtctg tacaactcgg agaaccacct
     1321 ctctggatat gacccagcgt tgtgactctt gtctcataat ccggcagtcg tctcttgacc
     1381 tgagcggcga tctccatcgc tccacctgca tcatctcctt cagctactat tactattccg
     1441 gaagtcttat ttttgcgacg ctcgttgagc aggtagtcgc agagcttttt aatatcggta
     1501 gggtattcgg ggaccaggac tgcctctgca cctgtaccta tggcagtatg taaagcaatg
     1561 aagccggcat cacgacccat aacctctaca aaaaagacca tattgtgtga atcggcagta
     1621 tctcgtaact tgtcaacagc ttcaagcgca gtgttaatgg ctgtatcaaa accgatagtg
     1681 tagtcagtac cataaatatc attgtcgata gtaccgggta ttcccactac cggaaaatca
     1741 aactctttga aaagatcgtt ggcgccccta aaagaaccgt caccacctat gacaaccaac
     1801 gagtctatac cggcctcacg catattatcc accgccgacc tacgcacttg gggatcattg
     1861 aactcaggac aacgggaaga tttgaggatg gtgcctccct gagagataat cttggatacg
     1921 gagtggctct gcatccccat aaactgtctt tcaaccagac cggtataacc tttacggatg
     1981 cctaccacct cgcaatgata aaatattgcc gaacgaacca ccgctctgat tgcggcattc
     2041 atgcccggag cgtcaccccc tgaggtgagc acacctattc ttctgattct gttttccatt
     2101 ttcaaatata ttttgggcaa aagtagtgtt tttactctat aatatctatt tttgcggtgg
     2161 attttcacca ttagagcccg atgaagattt caactcttga tctaggagag aagccactgc
     2221 tattggcacc tatggaggat gtcaccgacg cctccttccg ttgtatatgc aaggattatg
     2281 gcgcagatat ggtctatacg gagtttgttc cctcagacgg acttatacgg gacgcccgta
     2341 aggctctttc caaacttgtc atatatgatt atgagcgacc gataggcata cagatttatg
     2401 gtcacatccc cgaatcaatg gtggaagcgg cgaaaatggc ggaagaagca aaacccgaag
     2461 tgattgatct aaattttggc tgccccgtca acaagattgc caaacgtggt gcaggtagcg
     2521 gtatgatgcg agagcctgac aaacttgtag aaattacccg cagagtggtg gaggcggtca
     2581 aactgcccgt gacggttaag acgcgtcttg gctgggatga ggagtccaaa ataattgtag
     2641 atcttgctga aaggctacag gatgtgggga tagctgcgct cacgattcat gggcgcacac
     2701 gctctcagct ctataaggga gaggcagact ggactctgat aggtgaggtg aagcgcaacc
     2761 ccagaatgcg aattcctatt ataggtaacg gagatattac aactcctgag gatgcggcac
     2821 aagcctttga taggtatggt gtggatgcta taatgatagg aagggctact tttggacgcc
     2881 cttggatttt caaagagata cgtcactttc tcaatacggg tgagcaactc ccaccgataa
     2941 gcgtggccga aaaggtggag cttgctaaaa agcatcttgc aaaatccatc gaggtaaaag
     3001 gcgatagagt cggtcttctg gagatgcggc gtcatctcag ttcctatttt aaaggtctgg
     3061 ataatttcaa ggagaccagg ttgaaacttg ttaccacaaa tgaccctgcc gagctgatca
     3121 ccgttcttga ttttatcaat gaaaaatggc agtaaaatag tttggagttt ggagtatgta
     3181 gtgtggagtg ggagttggcg ctaagaagtt ttagtttgaa ttttgagtgg gtagtgtgta
     3241 gaaagtagga gaatatggag taatgatatt tgacttggaa agttaagagc aattggaaag
     3301 tagaagttaa gagtttaaaa taaacaaaat aagaagctaa cggccaacag ccaacagcca
     3361 acagccaaag agagttagta ttaaaaatga taataagtaa agttttacta ttcccatatt
     3421 atttgacgtt aaagttgagg aatcgcctat atgataaagg cgtcttcaag tcacaacaat
     3481 ttgatatacc cgtaatttca ataggtaaca ttaccgcagg gggtacgggt aaaaccccca
     3541 ctactgagct gttagttcga ctgctgaagc cccaccataa ggtagcggtg ctatcgcgtg
     3601 gctataaacg taaaacaaga ggatttagaa tagtggatac cgactccact tcacgtgaag
     3661 tcggggacga accgttacag atgagaaaaa agcttgaaga cgtcatcgtt gcggtggata
     3721 ccaacagaaa aaggggcata acacaactgc tcgcgctacc ccaggaaatg cgccctgatg
     3781 ttattgtttt ggatgatgct taccagcata gaaaagtcac tcctctgaaa aacattctac
     3841 tgatagatta caaaagaccc atatttaggg ataaccttct ccctatcggt agcttaagag
     3901 atctcccgga acaaatatgc agagcagacg ctattataat taccaaaagc cctgaacacc
     3961 ttgacgaatg ggaacgtgaa acaataaaaa aagccaaccg tatcaatagt gactctccct
     4021 gtttctttgc cagaataaaa taccgtccca tagagcctat cttcccggag ctgggcgaca
     4081 agagatacat ctactctaaa gaggtcggac tctttaccgg catagcaaat gtgagacccc
     4141 tgcttttgca cctatctgac atctatgaac agattaccca cacctcctac agagaccacc
     4201 atgagtttac acgtagcaat atccatgata ttgaatattt tgcaaggagc aatccccagg
     4261 ctctgatact aaccatggaa a
//
LOCUS       DAOSIE010000108         4239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_18424_length_4239_cov_3.749283, whole genome shotgun sequence.
ACCESSION   DAOSIE010000108 DAOSIE010000000
VERSION     DAOSIE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18424_length_4239_cov_3.749283"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..3376
                     /locus_tag="PLI69_04225"
     CDS             269..3376
                     /locus_tag="PLI69_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG41728.1"
                     /translation="MKRIKFLFALAAILLTGVVSAQNITVKGTVADRETGEPIPFASI
                     QVKGTTTGASADIDGRFSLNVPSNATLIFSFVGYKTIEVPVSGRSTIHVDLEVDTETL
                     EEVVFVAFGTVAKKDFTGSAVALESKKLESRPLTNVANALEGLAAGVQFTSAGGQPGS
                     SGGIRIRGFGSINASSSPLYVVDGVPFDAISNLNADDIENITILKDAASSALYGSRAA
                     NGVVLVTTKKGRSEKVQFNVKVNQGISMRAIQEYERLDPFQYYPMEWEALRNAYLSSS
                     GSTYTMETASIEASKKIYSQLLNNPFNVANDQIVGVDGKLNPAAKLLYTDDLDWAKGI
                     ARIGHKQEYSLSAGGATAKTDYYMSIGYMDEEGFSIKSYMERANARLNMNVQPRKWVK
                     AGINLAATMANSNVTSDGSSTAYVNPFYFSRFIGPIYPIYEHDAAGAIKLDDKGEKIY
                     DWTNRGASASPGRHVIAETLWNDNLYKRNLVNARAYIDIILFEGLKFSVNGGYDFRNY
                     LNSNYENPHVGDGAPAGRGSITNYRYDTYNFNQLLTYVKSFNNHNIDVLLGHESYKND
                     YKYVYGMKQGLIAEGNAELINFTTINSLYTYSQAYRTEGYMARANYNYNHKYYVSASF
                     RRDATSRFYKDARWGNFWSVGGSWRLDQESFIASQPWISALKLRASYGSVGNDGTDSW
                     YQWQSLYGILNNANASGFIQSTQAGNKELQWEKNRSFDVALEFGFFDNRLNGQIEFFH
                     RISDNLLFEVPLPTSSGVLSQWQNIGSMYNQGIELQLGGDVVRTKDVTWNILVNASHI
                     KNEITKLPQEEIISGTKKYMVGHSMYDFWLRKWEGVDPETGDALYLKDIKDAEGNVTG
                     SEIVNDYTIADYYYVGTSIPDLYGSIQNTLTWKGLELSVLTTYQIGGQVYDSVYGSLM
                     SHSGYGSAMHIDILKRWQKPGDVTEVPRIDTAKNPNQNAGSSRWLTSASYFSFKNITI
                     AYSFPRKWAEKLDLSGIRIYASGENVYAFTARKGMDPQYSFAGTQSNVYSPARNITFG
                     LNVQF"
     gene            3417..>4239
                     /locus_tag="PLI69_04230"
     CDS             3417..>4239
                     /locus_tag="PLI69_04230"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG41729.1"
                     /translation="MKNIIKILACLAVFAGIASCSPDFLETTPTSAYDASFIFTTTEN
                     ALGALNGIHKSMVSQYYSRQNISGYPSFQIAMDAMGEDLVFPTAGNGWWTNTGEVKWN
                     GQRNANGWLTAFPFRIAYKWISNANQILDNVDNAVGPDADKQMIKGQALAYRGMAYFW
                     LVQLYGDRYEPGKQNNQLAAPLVVNTVEVKKPLETVENIYAQIVTDLEQSITLLGTSS
                     FTSKTHFVAATVMGLRARVALAMHDWPNAKKYAEMAINTTCAKLMSQSDYTKGFND"
BASE COUNT         1201 a          956 c          964 g         1118 t
ORIGIN      
        1 gttgtaataa ctgatactaa atcgggtgtt acatcagcac ctgcccaggt gaagattatc
       61 gatacattca aaggtaaagt aggtagtgaa agagcttcca aagctaactt cattgtagaa
      121 aactaatctt tgctaatata ttttaactgg gggtggccaa agccatcccc agtttttttt
      181 ctctcattgt tggagatttc aaagatttgt ttatatttgc actctacaca cataagaaaa
      241 actttattaa ccaatacaaa tatattctat gaagagaatt aagtttctgt tcgctcttgc
      301 agcaatattg ctgacagggg ttgtttctgc tcagaatatc actgttaaag gtacagtggc
      361 agatagagag acaggtgagc ctatcccttt tgcatctatt caggtaaagg gtacgaccac
      421 cggtgcttcg gctgacatcg atggccgttt cagcttaaat gttccctcaa atgccactct
      481 tatcttcagc ttcgtgggtt ataagaccat cgaggtaccg gtatcaggca gaagcacaat
      541 tcacgtagat ctggaggttg acactgaaac actcgaggaa gtggttttcg ttgccttcgg
      601 tacagtagcc aaaaaagact ttacgggatc ggcagtagcc ctcgagagta aaaaacttga
      661 gagtcgtccc cttaccaacg tggcaaacgc attggaaggt ctcgctgccg gtgtgcagtt
      721 tacttctgca ggcggacagc ccggttcatc cggaggaatc cgcatccgtg gttttggatc
      781 tatcaacgcc agttcatctc ctctatatgt ggtggacggt gtaccatttg atgccatctc
      841 caaccttaat gcggatgaca tcgagaacat caccatcctc aaggatgccg cttcttcagc
      901 tctttacgga agccgcgctg ctaacggtgt ggtactcgta actaccaaga agggccgcag
      961 tgagaaggtg cagttcaatg ttaaggtaaa ccagggtatc agtatgagag ctatccagga
     1021 gtacgaaagg cttgatccat tccagtacta tcctatggag tgggaagcat tgcgcaatgc
     1081 ttatctttca tcttccggct cgacctatac aatggaaacc gcttccattg aggcttcgaa
     1141 gaagatttac tcacaactgt tgaacaaccc cttcaacgtg gctaatgacc aaatagttgg
     1201 cgttgacggc aagctcaacc ccgcggcaaa acttctctat actgatgacc ttgactgggc
     1261 caaaggcatc gcacgtatcg gtcacaaaca ggagtattca ctctcagcag gtggagcaac
     1321 cgccaagacc gactactata tgtcaatcgg ttatatggat gaggagggtt tctccatcaa
     1381 atcttacatg gagagagcca atgcgcgtct gaacatgaat gtgcagcccc gcaagtgggt
     1441 taaagccggt atcaatctcg cagctaccat ggccaattcc aacgttacca gcgatggtag
     1501 cagtacagct tacgtcaatc ccttctactt ctcaaggttc atcggcccta tctatcctat
     1561 ctatgagcac gatgccgcag gtgccatcaa actcgatgat aagggagaga agatttatga
     1621 ctggaccaac cgtggtgcaa gtgcaagtcc cggtcgtcac gtaattgctg agactctctg
     1681 gaatgacaac ctctacaaga gaaatctggt taacgcgaga gcctatatcg atatcatttt
     1741 atttgaggga ttaaaatttt ccgtaaatgg tggttatgac tttagaaact acctcaattc
     1801 caactatgag aaccctcatg tgggtgacgg tgctcctgca ggccgtggca gcattaccaa
     1861 ttaccgctat gatacataca acttcaacca gttgttgacc tatgtaaaat cattcaacaa
     1921 tcacaatatt gacgtactcc tcggacacga gagttataag aatgattaca aatacgttta
     1981 cggtatgaag cagggcctca tagcagaggg taatgccgag ctgatcaact ttactaccat
     2041 caactcactc tacacctatt cgcaggcata caggacagag ggttatatgg cacgtgccaa
     2101 ctacaactac aaccacaagt actatgtatc cgcatccttc agaagggacg caacctcacg
     2161 cttctataaa gatgcacgtt ggggtaactt ctggtcggta ggtggatcct ggagactcga
     2221 tcaagagtct ttcattgcaa gtcagccctg gatatctgct ctgaaactta gggcatctta
     2281 tggctcggta ggtaacgacg gtaccgactc atggtaccaa tggcagtcac tctacggtat
     2341 ccttaacaat gctaacgctt ccggtttcat acagtcaacc caggcaggta ataaggagct
     2401 ccagtgggag aaaaaccgca gctttgacgt tgcattggag tttggtttct ttgacaaccg
     2461 tctcaacgga cagatagagt ttttccacag aatttccgac aaccttcttt tcgaggttcc
     2521 gctccctacc tcttcaggtg ttctttcaca gtggcagaat atcggttcaa tgtataacca
     2581 gggtattgag ctgcagcttg gaggtgatgt ggtacgcaca aaggatgtta cctggaatat
     2641 tttggtaaat gcctcccata tcaagaatga gatcacaaag cttcctcagg aagagatcat
     2701 cagtggcaca aagaaatata tggtaggtca ctctatgtat gacttctggc tcagaaaatg
     2761 ggaaggtgtg gatcccgaaa caggagacgc actctatctt aaggatatca aggatgctga
     2821 gggtaatgtt acaggcagtg agattgtaaa tgattacacg attgcagact actactatgt
     2881 aggcacttca attcccgacc tttacggttc gattcagaat acacttacct ggaagggtct
     2941 ggaactctcc gtacttacta cctaccagat tggcggtcag gtatatgata gcgtttacgg
     3001 ctcattgatg tctcactccg gatatggttc agccatgcat attgacatac tcaagaggtg
     3061 gcagaagccg ggtgatgtta ccgaggttcc cagaatagat acagctaaga accctaatca
     3121 gaatgctggc tccagcagat ggcttacaag tgcctcatac ttctcattca aaaacataac
     3181 cattgcatac tcattccctc gtaagtgggc tgaaaagctt gacctttcag gaatcagaat
     3241 ttatgctagt ggtgagaacg tgtatgcatt cactgcccgc aagggtatgg accctcagta
     3301 ttcgtttgcc ggtactcagt ccaacgttta ttcccccgca cgtaacatca cgttcggttt
     3361 gaatgttcaa ttttaatcag cgaaaatcaa ccattaacaa acaagaataa tacagtatga
     3421 aaaatattat aaaaatattg gcttgtcttg cggttttcgc aggtatagct tcctgtagtc
     3481 ctgattttct tgaaactacc ccgacatcag cctatgatgc aagtttcatt ttcactacta
     3541 cggaaaatgc tttaggagct cttaatggta ttcataaatc catggtgagc caatactatt
     3601 caagacagaa catcagtgga tatccctcat tccagattgc aatggatgcg atgggagaag
     3661 atctcgtgtt ccccactgca ggtaacggat ggtggacaaa caccggtgag gtgaaatgga
     3721 atggccagag aaatgcaaac ggctggctta ctgcattccc cttcagaatt gcttacaagt
     3781 ggatttccaa tgccaaccag atcctcgata atgtggataa cgccgtaggc ccggatgccg
     3841 ataagcagat gatcaaaggt caggcactcg catatagagg tatggcttat ttctggctgg
     3901 ttcaactata tggtgacaga tatgagccgg gtaagcagaa caaccagctt gctgctccac
     3961 ttgttgtaaa taccgtagag gtgaaaaaac cgcttgagac agttgagaat atatatgcac
     4021 agattgtgac cgaccttgag caatcaatta cactgcttgg tacgagctca tttacatcaa
     4081 aaacgcattt tgtggccgcc actgttatgg ggcttagagc acgtgttgct ctggcaatgc
     4141 atgactggcc aaatgccaag aagtatgcag agatggcaat caataccacc tgtgcaaaac
     4201 tgatgagtca gagtgattat acaaagggct ttaatgatg
//
LOCUS       DAOSIE010000109         4175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_18803_length_4175_cov_3.881068, whole genome shotgun sequence.
ACCESSION   DAOSIE010000109 DAOSIE010000000
VERSION     DAOSIE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18803_length_4175_cov_3.881068"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1030)
                     /locus_tag="PLI69_04235"
     CDS             complement(128..1030)
                     /locus_tag="PLI69_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG41730.1"
                     /translation="MTKPQSKLPLYISIIIATILWGFSFIWTNDLIAMGVPIFIFIFI
                     RMLLSGSIMLAFSTLTRTLQKVARKDIKWMFLLAFFEPFIYFIGESYGLKITGSATLS
                     AVIIATIPIFTMIMGRLLFNEALSRLNIAGIITAVLGIVLFVWNGTISSENYYGILFL
                     LLAVAGAVGYSSICKRLTSTYNAITITTWQFLFGAALFFIPFMIWGLPQWRPEYLSLD
                     VLKPLITLAVLCSSFSFVLYTKAIEGLGMTKAAIFTTMIPAVSAVAAYFLGQESFTTL
                     QIIALVIALSGVVLSQLRKSLKII"
     gene            complement(1023..2354)
                     /locus_tag="PLI69_04240"
     CDS             complement(1023..2354)
                     /locus_tag="PLI69_04240"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOG41731.1"
                     /translation="MSIFIENGTIINEGLSFTGSLFVENGIISAITDDPTAFSHLREK
                     AGEVIDAAGMLILPGVIDDQVHFREPGAEHKGSIATESAAAVLGGVTSYMDMPNNNPP
                     TCSQELLASKFAKAAQDSLANYSFYIGASNDNLAELLATDPHNVCGIKVFMGSSTGNM
                     LVDNPASLDSIFSRAHLLIATHCEEEAIIVANTAAARERFGDAIPIAMHPEIRSREAC
                     IASTRKAIDLALKYSSRLHILHISTAEEVELLREARERSGRITGEVCVHHLWFSDKDY
                     EQYGTLIKCNPAIKSASDREALRQAVREGVISVVATDHAPHLLSEKEAKYLQSPSGIP
                     LVQHSLRAMLELAHQGHFSLTDVVRLMCHSPADCFTISRRGYLREGYFADITIVDPNK
                     PSDATNVYRCGWTPFTRFSSTVMHTLVNGIPVVRDSQLTGLKSSLPLSFDD"
     gene            complement(2370..3101)
                     /locus_tag="PLI69_04245"
     CDS             complement(2370..3101)
                     /locus_tag="PLI69_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOG41732.1"
                     /translation="MTRQIIIIPTYNEKENIEKIIRAIREQEVDFHILVIDDGSPDGT
                     AAIVKSLQSEFPDSLFIIERAGKQGLGTAYITGFKWAIEHKYDYVFEMDADFSHPPKD
                     LPRLYKACHEDGADLAIGSRYCNGISVVNWPIGRVIMSYYASTYVRTVLGMKVYDTTA
                     GFKCYRRKVLETIDFSNIRMRGYGFQIEMKYNTYKLGFKIVEVPIIFVDRTEGTSKMS
                     SGIFGEAFWGVLKLRFRKIKPAPKA"
     gene            complement(3145..>4175)
                     /locus_tag="PLI69_04250"
     CDS             complement(3145..>4175)
                     /locus_tag="PLI69_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG41733.1"
                     /translation="YKIEVIAIGNGTAGRETEAFIKKIPLPQGVRVYSVSEDGASVYS
                     ASQIAREEFPDYDVTVRGAVSIGRRLMDPLAELVKIDPKSIGVGQYQHDVNQTLLKER
                     LDTVVESCVNNVGVNLNTASKHLLRYVSGIGPVLAANIIEYRDENGAFGSRQELLKVR
                     RLGEKAFEQCAGFMRIPNGNNPLDNSAVHPERYALVEQMASDLGVSLPDLISNEQLSA
                     SIDISKYLSDDVGEHTLNDIKKELAKPGRDPRSIIKVFEFSPEIRSIEDVKVGMILPC
                     IVTNITNFGAFVDIGIKQNGLIHVSQISEKRVEDPSQVLKLHQQLHAKVVDVDLERQR
                     IGLSLRGM"
BASE COUNT         1212 a          978 c          908 g         1077 t
ORIGIN      
        1 cttccaattt tatcaaggaa cggacgtctc ttttaagaaa cgggttgcaa agataagaac
       61 atttttaaaa ttagcaaaat aaatttggaa gttttccccc aaaaaagagt ataattctga
      121 gaaccaatca gataattttt aaactttttc gcaactgcga aagaactact ccggaaagag
      181 caattacaag tgcaataatc tgcaaagttg taaaactttc ctgccccagg aaataggcag
      241 ctaccgctga aaccgcggga atcatagttg taaaaatagc cgcttttgtc attcccagac
      301 cttctattgc ctttgtatag agtacaaaag agaaagagga acagagtact gcaagtgtaa
      361 tcaagggctt gagcacatca agcgagagat attccggtcg ccattggggt aaaccccaaa
      421 tcataaaggg gataaaaaac aaagctgccc cgaaaagaaa ttgccatgta gtgatggtaa
      481 tggcattgta ggtacttgta agtcttttac atatagatga gtaacctacc gcacctgcaa
      541 ctgcaagcaa aaggaagagt atgccgtaat aattctccga agaaatggtg ccgttccata
      601 caaatagcac tattcccaaa acagcagtta ttataccggc tatgttgaga cgagaaagag
      661 cttcattgaa caacaatctg cccataatca ttgtaaaaat gggaattgtt gcaattatca
      721 cagccgaaag agtagcagaa ccggtgattt tcaaaccata ggactcccct ataaaataga
      781 tgaagggctc gaaaaaggcc aacagaaaca tccacttgat atcctttcgc gccaccttct
      841 gcaaagtacg ggtaagcgtg gaaaaggcaa gcattatgct ccctgagagc agcatcctta
      901 taaaaatgaa tataaagatc ggcaccccca ttgctatcag gtcgttggtc cagataaagg
      961 agaaacccca cagtatggtg gcaatgataa tggatatata gagaggaagt ttactttgag
     1021 gcttagtcat cgaaagagag tggtaatgaa gatttcagac ctgtaagctg tgaatcgcga
     1081 accaccggaa tgccatttac cagagtatgc atgaccgtcg aactgaagcg agtaaaaggt
     1141 gtccagccgc atctgtaaac gttagtagca tctgaaggtt tattgggatc tacgatggtt
     1201 atatctgcaa aatatccctc gcggagatat cctctgcggg agatagtgaa acaatctgcc
     1261 ggagaatggc acatcagtcg cacaacatcg gtgagtgaaa aatgaccctg atgcgcaagt
     1321 tcgagcattg cacgaagcga atgctgtacc agagggatac cggaaggcga ctgaagatat
     1381 ttagcctctt tttccgacaa taggtgtggc gcatggtcag ttgccactac ggagatcacc
     1441 ccttcacgca ctgcttgacg aagcgcctcg cggtcggaag cgctctttat ggcgggatta
     1501 cacttgatca gagtgccata ttgctcatag tctttatcag agaaccatag gtggtgtaca
     1561 catacttcac cggtaattcg accgctccgt tctcttgcct cacgaagtaa ctccacctct
     1621 tcggccgtgc ttatatgaag tatgtgcaga cgcgaagagt acttaagtgc caaatctata
     1681 gccttgcggg tagaggcaat gcaagcctct ctgctgcgaa tttccggatg catcgcaatc
     1741 ggtatggcat caccaaagcg ctcacgggcc gcagcagtat ttgctactat gatggcctcc
     1801 tcttcgcaat gagttgcaat caagagatgg gctctcgaga atatggagtc caaggaagcg
     1861 ggattgtcca ccaacatatt tcctgtggaa gaacccataa aaacctttat accgcagaca
     1921 ttatgcgggt cggtcgcgag aagctcggca agattgtcgt tcgaagcacc tatataaaat
     1981 gaataattgg caagtgagtc ctgcgctgcc ttcgcgaact tgctagcaag tagctcctga
     2041 gagcaagtcg gcggattatt gttgggcata tccatatagg atgtaacacc acccagcacc
     2101 gctgcggcac tttcagtagc gatgctaccc ttatgttcag cccccggctc acgaaaatgc
     2161 acctggtcat ctataactcc cggaaggatg agcattccgg cagcatcgat tacctcacct
     2221 gctttttccc gcaagtgcga aaatgctgtc ggatcatccg ttatagcact gatgatgcca
     2281 ttttcaacaa agaggctacc cgtaaaagag agcccctcgt tgatgattgt gccattttca
     2341 ataaaaatgc tcattgatat agttgtatct tatgcctttg gcgccggctt aatcttgcgg
     2401 aatctcagtt tgagtacccc ccaaaaggct tcgccgaaaa taccgctact cattttcgag
     2461 gtaccttcag tacggtctac gaaaataatt ggaacttcaa cgatcttgaa tccgagtttg
     2521 taggtattgt atttcatttc aatctgaaat ccgtaacctc tcatccttat atttgaaaaa
     2581 tctatcgtct caagtacctt gcggcggtaa cacttaaaac ctgccgtggt gtcatagacc
     2641 ttcattccaa gcactgtgcg aacataagtg gatgcataat aggacataat caccctaccg
     2701 ataggccagt ttactacgct gatgccatta cagtaacgtg aacctatggc cagatcggct
     2761 ccatcctcgt gacaggcctt ataaagacgt ggcaggtctt tgggtggatg tgaaaaatct
     2821 gcatccattt cgaaaacata atcatattta tgttcgatag cccatttgaa gccggtgata
     2881 taagctgtac cgagtccctg tttgcctgcc ctctctataa taaaaaggga atcgggaaac
     2941 tcactttgta gagatttaac aatagccgca gtaccgtcgg gcgaaccgtc atcgatgacc
     3001 agtatatgga aatccacttc ctgctccctg atagcacgga taatcttttc tatattctcc
     3061 ttttcgttgt aggtaggaat aatgataatc tgtcgtgtca taatctctag aatattaagt
     3121 ttatcttgta aagatagtaa aaaatcacat tcctctcaaa gaaaggccaa ttctctgacg
     3181 ttctaaatcc acatccacca cttttgcgtg aagctgctga tgcagtttca ggacttgcga
     3241 gggatcttcc acacgctttt cgcttatctg tgatacatgt ataagaccgt tttgcttgat
     3301 acctatatcc acgaaagcac cgaaatttgt gatatttgtt acgatacagg gcaggatcat
     3361 acctaccttt acatcctcta tggagcgaat ttcaggggag aactcaaaca ccttgataat
     3421 cgaacggggg tcacggccgg gcttggccaa ttccttttta atatcattaa gcgtatgctc
     3481 acccacatca tcggagagat attttgaaat atcaatagaa gcggataact gctcattgga
     3541 aataagatcg gggagcgata cacccaagtc tgaagccatc tgctccacga gagcataccg
     3601 ttcgggatga acagccgaat tatcgagcgg attattacca ttaggtattc tcataaaacc
     3661 ggcacattgc tcaaatgcct tttcgccgag ccttcgtacc ttgagcagct cttggcggct
     3721 gccgaatgcg ccattttcat cgcgatactc tataatattg gccgctaata ccggacctat
     3781 gcctgaaaca taacgcagca ggtgcttgct cgccgtatta aggtttactc ctacattatt
     3841 gacacagctc tccaccaccg tatccaagcg ctccttcagt aaagtctggt tgacgtcatg
     3901 ctggtactga cccaccccga tggacttggg gtcaattttt accaattcgg ccagagggtc
     3961 catgaggcga cggcctatgg aaacagctcc cctcaccgta acatcgtaat ccggaaactc
     4021 ctctctcgcg atctgggatg ccgaataaac agaagctcca tcttcactta cagaatagac
     4081 tctcaccccc tggggaagag gaattttctt gataaaagcc tctgtctcac gaccggcagt
     4141 accatttcca atggcaataa cctctatctt gtagg
//
LOCUS       DAOSIE010000110         4166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_18851_length_4166_cov_3.955242, whole genome shotgun sequence.
ACCESSION   DAOSIE010000110 DAOSIE010000000
VERSION     DAOSIE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18851_length_4166_cov_3.955242"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..2612
                     /locus_tag="PLI69_04255"
     CDS             231..2612
                     /locus_tag="PLI69_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOG41734.1"
                     /translation="MKKRIWIVAISVFVLLISGCERWDIDKITEPGVSRELADLRHNT
                     ISNLNYDLSFEIPGNAEEDIPAVITIAFDFNSVGKYPFLLDFKEKPEMVHSLELNEDS
                     CDYLFENEHIIIPSSLLKRGRNSIRIAFSAGKQSLNRRDNFMYTLLVPDRARTLFPCF
                     DQPDLKASYILTLSVPDKWKAVANSPLNKSVTNADNQTVTYSFHPTEPLSTYLFSFVA
                     GEFEKAGKSDNLGRTIGLYHRETDPYKLAQIDEILDEVFYSLEWLEEYTDIPYPFAKY
                     DCIVLPGFQYGGMEHTGATLYNDRHIFLEKGAGISEKMNRFSLIAHETAHMWFGDYVT
                     MKWFDDVWTKEVFANYFAALISADKYPDVDNSFTFLDYASAAYSVDRTDGANAIKQPL
                     ANLKDAGLIYGNVIYNKAPIVMEMLSRKMDSEAFKRGIREYLNRYAYGNADWEGLISI
                     LDKFTDENLAAWSNDWVHKPGMPHYKVDSLSQIDLNGLNYGYYELSEELSTALMNNLS
                     TQPLNPPEKASTLVILYENYLNEKITGFDYTLFLLDCLESLSKEDSSQTTLVFRRALS
                     QLGSILWKENELEATEWEERLKELISESLSESCRRAAFSALLNAPHSGSVTEMFLHAF
                     LEPKSFNYFQLTNADLTQLCQQLAVRKERIAPWLITIQRARLSHPDLIAQFDYIAPAL
                     ASTPEARQECFQSLLKVENREVEPWTLTVLRLLNHPLRQQEALSYIRPALGIIEEIQI
                     TGDIFFPTNWCSALLGGHHSKEAKNEVRLYLEEHSKTLNPLLVQKILQAAYYI"
     gene            2675..3739
                     /locus_tag="PLI69_04260"
     CDS             2675..3739
                     /locus_tag="PLI69_04260"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOG41735.1"
                     /translation="MNKDFKIQSIGLMSGTSLDGLDVCCCTFTRKNGKWNFTIDCAKG
                     YSYPDDMKYILGTGAQQMSALDFITFHSAYGKFLGEKVNLFMEEFGVKPDIIASHGHT
                     IFHEPHKKVMYQIGDGAAIAAETGIPTVSDFRRLDIMMGGQGAPLVPIGDRMLFSDYD
                     YCLNIGGFSNISFEKEGQRVAFDISPVNYVFNHYTRQIGLEYDKDGDIARSGKVCQPL
                     LEELNGLEFYHTAGPKSLGREWVEELIFPMIDKYGLSLADRLRTFCEHAAFQVSRVTS
                     PGKRLLITGGGAFNKFLVERISTLTGCEIVIPDPIIIEYKEALIFALLGALYMADQPS
                     CLSSVTGASRDNIGGMLFKI"
     gene            3863..>4166
                     /locus_tag="PLI69_04265"
     CDS             3863..>4166
                     /locus_tag="PLI69_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41736.1"
                     /translation="MKTRIVLFVMIATLILGAGCEKMPTDTRNITLYNQPLSVIKHCI
                     QGKWKVVYEKGGFIATRIQYWDDYYMEFTKNNRMIVSRDGVADYQNTKIRWYKECYC"
BASE COUNT         1193 a          877 c          920 g         1176 t
ORIGIN      
        1 tgtagtatgt agtatgtagt atgtagtatg tagtgggtag tatgtagtat gtagtatgta
       61 gtatgtagtg ggtagtgggt agtgtgtagt attgagtatt gagtatcgag tattgagtat
      121 tgagtattga gtatcgagta ttgagggtgt agtgtggagt gtaattactc gtaactcgca
      181 acaagcaact cgtaactcgc aactcataac taacaataaa atattttact atgaagaaac
      241 ggatttggat agtcgcgata tcggtgtttg tactactgat atcgggttgt gaaagatggg
      301 atattgataa aataaccgaa cctggagtaa gtcgtgaact tgctgatttg cgccataata
      361 caatatccaa tctaaactat gacttgagct ttgagattcc cggtaacgct gaggaagaca
      421 ttccggctgt gataaccatt gcatttgatt ttaacagtgt cggaaagtat ccgtttctgt
      481 tggacttcaa agaaaagcct gaaatggttc attctctgga gctgaatgag gatagctgcg
      541 attatctatt tgaaaatgaa catatcatca ttccttcttc tctccttaaa aggggaagaa
      601 acagtatcag gatagccttc tctgcaggaa agcaatcatt gaacagaagg gacaacttta
      661 tgtacacatt gctggtacct gacagggcca ggaccctctt cccctgtttc gaccaacctg
      721 acctaaaagc ctcctatatt ctaactctct ctgtaccaga taaatggaag gcagtagcca
      781 atagtcctct aaacaaaagt gttacaaacg cagataatca gacagttacg tattcatttc
      841 atcccacaga gcctctgagc acatatctct tctcttttgt cgcaggtgag tttgaaaaag
      901 ctgggaaaag tgacaattta ggtcgtacaa taggacttta ccatagggaa acggatcctt
      961 acaaacttgc acagatagac gaaatactgg atgaggtctt ttattcattg gaatggctcg
     1021 aagaatatac cgatatcccc tacccctttg caaagtacga ctgtatagtc ctgcccgggt
     1081 tccaatacgg agggatggag catacgggag ctactcttta taacgaccgc cacatatttc
     1141 tggagaaagg cgcaggcatt tccgaaaaga tgaacagatt cagtctgata gcacacgaaa
     1201 ctgcccatat gtggttcggg gattatgtta cgatgaagtg gtttgatgat gtttggacaa
     1261 aagaggtttt tgccaattac tttgcagcat tgatctctgc tgacaagtat ccggacgtgg
     1321 ataattcctt cacattcctc gattacgcat ctgctgctta ctccgttgat cgtacagatg
     1381 gagcaaatgc catcaaacaa ccgcttgcca atctaaaaga tgcaggcctc atttacggca
     1441 atgtcattta taacaaagct cctatcgtaa tggagatgct ctcaaggaaa atggattctg
     1501 aagccttcaa gagaggtatt cgcgaatatc taaatagata tgcttatgga aatgccgatt
     1561 gggagggcct gatatccatt cttgataaat ttactgatga aaatcttgcc gcatggagca
     1621 acgactgggt tcacaagccc ggaatgcctc attacaaagt ggattctctt tcacaaattg
     1681 atctgaacgg gctcaattac ggctattatg agctctctga ggagctttct actgctttaa
     1741 tgaacaatct ctccactcag cccttgaacc ccccggaaaa ggcctctacg ctcgttattc
     1801 tctacgagaa ttatctaaat gagaagatta cagggtttga ttatacatta ttcttgctcg
     1861 attgtctcga atcactatca aaagaggatt cgtcacagac gacgctggta ttcaggcggg
     1921 ccctctccca attgggaagc atactatgga aagaaaatga actggaagca accgaatggg
     1981 aagagcgtct gaaggaactt atatctgagt cgttatccga gagttgccgt agagccgcat
     2041 ttagcgctct gctaaatgct ccgcactcgg gatctgtcac agaaatgttt ctccatgcct
     2101 ttctggagcc gaagagtttc aactatttcc agcttacgaa cgctgatctt actcagctct
     2161 gtcagcaact tgcagtcaga aaagagagaa ttgccccgtg gctgattaca attcagcgtg
     2221 cacgcctctc ccaccccgat cttattgcac agtttgatta tatagcaccg gcactcgcct
     2281 ccactccgga agcccgacaa gagtgtttcc agtccctttt gaaggtagaa aaccgcgaag
     2341 tggagccctg gacgctgaca gtgctacgcc tgctcaatca tccgctcaga cagcaggagg
     2401 cgctctcata tattcgcccc gcgctgggta ttattgaaga gatacagatt accggagaca
     2461 tattctttcc caccaattgg tgctccgcgc tacttggcgg tcatcattca aaggaggcaa
     2521 aaaatgaggt taggctatat ctggaagagc attccaaaac tctcaatcct cttcttgtac
     2581 aaaaaatcct acaggcagcc tactatattt agactcaatc tctcattttg ttacgttttc
     2641 gatattggtt atatttgtca ataaaaaaat atttatgaat aaagatttca aaattcaatc
     2701 catcggtttg atgtcaggca cctcgcttga cggactcgat gtttgctgtt gcacctttac
     2761 acgcaaaaat ggaaaatgga atttcacgat agactgcgcc aaaggctaca gttatcctga
     2821 tgatatgaag tatatccttg gaacaggcgc ccagcagatg agtgcactcg attttataac
     2881 atttcacagt gcttatggga agtttctcgg agaaaaggtc aatctcttta tggaggaatt
     2941 cggcgtaaag cctgatataa tagcttccca tggccatacg atattccacg agccacataa
     3001 gaaagtcatg taccagatag gtgacggagc tgcaattgca gcagaaaccg gtatccctac
     3061 tgtttcggat ttccgcagat tggatataat gatgggcggc cagggagctc cgctggtacc
     3121 tataggtgat agaatgctct tttccgatta cgattattgt ctaaatatcg gcggtttttc
     3181 caatatttcc tttgaaaaag aggggcaacg tgtggcattc gatatcagcc ccgtcaatta
     3241 tgtttttaac cattacactc gtcagatagg gcttgagtat gacaaggatg gggatattgc
     3301 acgcagtgga aaagtttgtc agcctcttct cgaagagttg aatggcctgg aattctatca
     3361 cacagccggt cccaaatcac tcggacgcga atgggttgag gaacttatat tccctatgat
     3421 tgataaatat ggcctctcgt tagctgacag gctgcgtaca ttttgcgaac atgccgcatt
     3481 tcaggtgtca cgcgtcacct cacctggcaa gcgtttgcta ataaccggag gcggagcatt
     3541 caataaattc ctcgtagagc gcatttccac cctcacaggc tgcgaaatag taattcccga
     3601 cccgatcatc atcgaatata aagaagccct tattttcgct cttttaggag cattatacat
     3661 ggcagatcag cccagttgcc tctcctccgt caccggagcc tcccgcgaca acatcggcgg
     3721 aatgttgttt aagatatagt attgaaattg agctcggtat aacaccgggc tttttcattt
     3781 tagcctctta cctatgcaac gtttttgcta ctttttgcat cttataggta agtacttggt
     3841 ttattagcag caatatagca ctatgaagac gagaatagtc ctattcgtga tgatcgccac
     3901 tctaattctt ggagcgggtt gtgaaaaaat gccgaccgat acacgaaata ttactcttta
     3961 caaccaacct ttaagtgtta taaagcattg tattcagggc aagtggaaag ttgtgtatga
     4021 aaagggaggt tttattgcaa ccagaattca atattgggat gattattata tggaattcac
     4081 aaaaaacaat cgaatgattg tttccagaga cggagttgcc gactatcaga acactaaaat
     4141 aaggtggtat aaagagtgtt attgct
//
LOCUS       DAOSIE010000111         4153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_18933_length_4153_cov_3.190825, whole genome shotgun sequence.
ACCESSION   DAOSIE010000111 DAOSIE010000000
VERSION     DAOSIE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18933_length_4153_cov_3.190825"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLI69_04270"
     CDS             <1..929
                     /locus_tag="PLI69_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOG41737.1"
                     /translation="RIEKLPVVDENNRLVGLITYRDIMKIKKNPTASKDSRGRLLAVA
                     AVGINSHSLEDVEKLVSVDIDAVVLDTAHGHSLFVLQTIKKIRKAFPHLQIVAGNIAT
                     AEAAIALKECGVDGVKVGIGPGSICTTRIIAGIGVPQFSAVMNTAEALKGSGIPIIAD
                     GGVRYSGDIVKALAAGASTIMAGSLFAGVEESPGEAIILNGRKFKSYRGMGSLEAMQR
                     GSKDRYFQDMEEDIKKLVPEGIVAQVPYKGTLAEVIYQLIGGLRAGMGYCGAADLAAL
                     RNAKFVKITHAGMVESHPHDVTITRESPNYSR"
     gene            936..1790
                     /locus_tag="PLI69_04275"
     CDS             936..1790
                     /locus_tag="PLI69_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41738.1"
                     /translation="MQKVLLIIILMLIISGCSFIEKGGRGRYDRRVARIGHEVLYESD
                     IVRMMPDGVSSADSAAMVTRYINTWALGRLLLLKAEEELSKAERDVTAEVEEFRNTLL
                     GFRFEKRWVEERLDTAVTLEEARLYYEEHPSSSIFPYPLVKARVIKISTGSPYYETVK
                     GAYTVTSEQDVADLKELCYSSAERYTDFGREWVPLSQLAKELGTDIETCTREVLPRRS
                     SEMKFNDSNYLIFIEEKVGAGEVSPFEYNVARIRETILGKRKQELLTKLEQDLLEDAH
                     IDGTLKIY"
     gene            1796..3163
                     /locus_tag="PLI69_04280"
     CDS             1796..3163
                     /locus_tag="PLI69_04280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG41739.1"
                     /translation="MKHTLVATALFLIISLFGMDISAQRYQGGLIDKTIVLIGNDMIM
                     LSDLENEVQMMSARGFTADRSARCQILEEMLVSKLFLNQARIDSLDVNEATVESSLTQ
                     RINEVMTQLGGEKQMEEYFNKPLYKLRQEWKEMLTEQSLIQDMQRKVAGNIPNLTPSD
                     IEKYCKETPEEDLPIVSTQYRIRQIVLYPDKEAAELEVKERLLGLRERIVAGEKFSTL
                     ARIYSQDPGSYTKGGELGMASKTIFWPAFSDAAMSLKVGQVSQIVETPDGYHLIELIA
                     REGDMFNARHILIKPHYTTEDRVKAFDRLDTIRTRILGDSISFFNAARYFSEDRKTAT
                     NGGLLADEYSGSSYFEKDQLKPADYNVLKDMQEGDISEPFESLDNEGRSGNTVYKIIM
                     LEKIIPSHVADYKTDFNVLLDDANRKNSTAAIDKFISEKQKSTYIVIDPLFRECNFMR
                     EGWVK"
     gene            3167..>4153
                     /locus_tag="PLI69_04285"
     CDS             3167..>4153
                     /locus_tag="PLI69_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOG41740.1"
                     /translation="MNLIRKVFNIALLAFCTLPLAAQRNLSEEDIFRLVDAASVQQFE
                     EFGMNYRKVLGDPARFLHNNTFLLCDSAVWNVSAKYIEAYGNVRIVQDGTMLSSESAT
                     YLIDNNMVRFNGPLVELVDKEGNRLRSDRMVYNTKDSVATYESGGAMKDKEGSVIESR
                     TGIYDSKINTFTFDGEVEVYTDTIFMKSTTMRYLTDEKKIYFGHNTYTWRGDGFIKSD
                     AGWYDNSKGIIHFALDVYMNNDDYEAWCDEVYYHRETGRTEMYNNIQILNEKDYMTIL
                     GNKLIYTADTVATLTDRPAIAYYGENENHIVDSLFFGADTLHLYTVKRNYIPK"
BASE COUNT         1183 a          873 c         1033 g         1064 t
ORIGIN      
        1 atcgcataga aaagctcccc gttgtggatg aaaataatag actggtaggt ctgattacat
       61 atcgtgatat aatgaagatc aagaagaatc ccaccgccag taaagattct cgtgggcgcc
      121 tgctggcagt cgctgcagta ggcatcaaca gtcacagctt agaggatgta gagaagctcg
      181 tctctgtgga tatcgatgca gtagtcctgg atacggccca cggacactcc ctttttgtgc
      241 tacaaaccat caagaaaata cggaaagctt ttccccattt gcagatagtg gccggcaaca
      301 ttgctaccgc cgaggcagct attgccctta aagagtgcgg agtagatgga gtaaaggtgg
      361 gcatcggccc cggttccatc tgtactaccc gtattatagc aggtattgga gttcctcaat
      421 ttagtgcagt aatgaatact gcggaggccc ttaaaggaag tggaattccc atcattgcag
      481 atggcggtgt ccgctactcc ggagatattg tcaaggcgct tgccgcaggt gcgagcacca
      541 tcatggcagg atcgctcttt gccggagttg aggagtctcc gggagaggca atcatcctca
      601 atggccgtaa gttcaaatcc taccgtggca tgggttcact cgaggctatg cagagggggt
      661 ccaaagatag atatttccaa gatatggaag aggatatcaa gaagcttgtg cctgaaggaa
      721 tcgttgcgca ggtaccttac aagggcactc ttgccgaggt tatctaccag ttgatcggag
      781 gtttgagagc aggtatgggc tactgtggcg cagcagatct tgctgctctt cgcaatgcta
      841 aatttgtaaa gattacccat gcaggaatgg tggagagcca ccctcacgat gtgacaataa
      901 cccgtgaatc ccccaattat tccagataga ccgctatgca gaaggtactc ctgatcataa
      961 tattgatgct tatcatttcc ggctgctcct ttatagaaaa agggggcagg ggccgttatg
     1021 accgcagggt tgcaagaata ggtcatgaag tgctgtatga gagcgacatc gtgcgtatga
     1081 tgccggatgg cgtctcatct gcggacagtg cggcaatggt cacgcgttat attaatacct
     1141 gggcactggg tcgactgtta ctgctcaaag ctgaggaaga gctttccaag gctgagcgcg
     1201 atgttactgc cgaggtagag gagttccgta acactttgct tggcttcaga tttgagaagc
     1261 gctgggtaga ggagaggctc gacacggccg tgactctgga agaggcgcga ttatactatg
     1321 aagagcatcc ctcaagcagt attttcccct atccattggt taaggcgcgc gttatcaaga
     1381 tttctaccgg ttctccctat tatgagaccg tcaagggagc atacaccgtc acttcggaac
     1441 aggatgtggc tgacctcaaa gagctgtgtt actcttctgc tgagcgctac acggacttcg
     1501 gcagggagtg ggtgcctctt tcacaacttg caaaagaact gggtacggat attgaaacct
     1561 gcaccaggga agtgcttccc agaagaagta gtgagatgaa gtttaacgac tccaattatc
     1621 ttatatttat tgaagaaaag gttggtgcgg gtgaagtatc gcctttcgaa tacaatgttg
     1681 caaggattcg cgaaaccata ctgggcaaaa gaaaacagga actcctgaca aaattggaac
     1741 aagacttgct tgaagatgcc catattgacg gaacgctaaa aatttattga aaaatatgaa
     1801 acacactctt gttgcaactg cactctttct catcatatcc ctattcggaa tggatatatc
     1861 cgcgcagagg tatcagggag gattgataga caagacaatc gtgcttatag gtaatgacat
     1921 gataatgctc tctgatctgg aaaatgaagt gcagatgatg agtgccagag gcttcacggc
     1981 tgaccgtagc gcccgctgtc aaatacttga ggagatgctt gtttccaaac ttttcctaaa
     2041 tcaggcaaga attgactctc tggatgtcaa tgaagctact gttgagagtt cgcttacaca
     2101 gcgcatcaac gaagttatga cacaactagg cggtgaaaaa cagatggaag aatatttcaa
     2161 caaacccctc tataaacttc gtcaggagtg gaaagagatg ctaaccgaac aatcgctcat
     2221 tcaggatatg cagcgcaagg ttgcaggaaa tatcccgaat cttacacctt cggatataga
     2281 gaaatattgc aaggagactc ccgaagagga tctccccatc gtatctacgc agtaccgtat
     2341 tcgtcagata gtactctacc ctgataaaga ggctgcagaa ctcgaagtga aagagagact
     2401 tcttgggctt agagagagaa ttgttgccgg agaaaaattt tcgacacttg cgcgaatcta
     2461 ctcacaggat cccggtagtt atacaaaggg tggagagttg ggtatggctt caaaaaccat
     2521 attctggcct gccttttccg atgcagcgat gtcgctaaaa gtaggacagg tatcccagat
     2581 agtggaaacc cccgatggat accatcttat cgagctgatt gcccgggaag gcgatatgtt
     2641 taatgcccgt catattctta ttaagccaca ttatactact gaggatcgtg taaaagcatt
     2701 tgaccgcctg gacacaatca gaaccagaat tcttggggat tccatttcgt ttttcaatgc
     2761 tgcaagatat ttctcggaag accgcaagac tgctacaaat ggtggtttgc ttgcagacga
     2821 atactccggc tcctcatatt tcgagaagga ccagctaaag cctgccgatt acaatgtgct
     2881 caaagatatg caggaaggtg atatttctga gccttttgag tctcttgaca acgaaggccg
     2941 cagcggtaac accgtctaca agatcattat gctggaaaag ataattcctt ctcatgtggc
     3001 tgattataag accgacttca atgttctgct tgatgatgcc aatagaaaga attcaactgc
     3061 agctattgat aaattcattt cagaaaagca gaagagtacc tatatcgtca tagaccctct
     3121 tttcagggag tgtaacttca tgagggaagg ctgggtgaaa taatccatga atcttatcag
     3181 aaaagtattc aatattgctc tacttgcgtt ctgtacgctg cctttggcgg cgcagaggaa
     3241 tctatcggaa gaggatattt tccgtctggt agatgcagca tccgtgcaac agttcgagga
     3301 atttggcatg aattaccgta aggtactcgg agatcctgct cgtttcctcc ataataatac
     3361 ttttttattg tgtgattcgg cagtctggaa tgtcagcgcc aagtatatag aggcctatgg
     3421 caacgtaagg atagtacagg acggtacaat gctttcaagt gaatccgcta cctatctgat
     3481 tgacaataat atggtgcgtt tcaacggccc gcttgtggaa cttgtcgata aggagggcaa
     3541 tagactccgc tccgatagga tggtttacaa tactaaggat agcgtagcta cctatgagtc
     3601 cggaggtgct atgaaagata aagagggttc ggtcatagag agtcgcaccg gcatctatga
     3661 ctcaaagatc aatacattta catttgatgg agaggttgag gtctatactg ataccatctt
     3721 catgaaatcc acaactatgc gctatctgac ggatgaaaag aagatctatt tcggtcataa
     3781 tacctatacc tggagaggag atggcttcat taagtcagac gcgggatggt atgacaactc
     3841 caagggcata atacatttcg ctttggatgt ctatatgaat aacgatgatt atgaagcgtg
     3901 gtgtgacgag gtctattatc atcgtgaaac cggtcgtacg gagatgtaca acaatatcca
     3961 gatccttaac gagaaggatt atatgaccat cctgggcaac aaactgatat ataccgccga
     4021 tacggttgct acactaacag atcgtcccgc aatagcctat tatggagaga atgaaaatca
     4081 tatagtggac agtttgtttt ttggggcgga cactcttcat ttgtatacgg tcaaacgcaa
     4141 ttatattcca aag
//
LOCUS       DAOSIE010000112         4141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_19013_length_4141_cov_4.606706, whole genome shotgun sequence.
ACCESSION   DAOSIE010000112 DAOSIE010000000
VERSION     DAOSIE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19013_length_4141_cov_4.606706"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLI69_04290"
     CDS             complement(<1..359)
                     /locus_tag="PLI69_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41741.1"
                     /translation="MHIPENIPRNYVDNCFTSSAKAVYITSVISLMEHPSVHIVFADT
                     KKNAYFLTNDFYSFFSDECIYYYPSPTDKNELKEATAKVQRTATLSALNSFEGASDAS
                     WSEKVGSGSFIINDRLL"
     gene            complement(361..1590)
                     /locus_tag="PLI69_04295"
     CDS             complement(361..1590)
                     /locus_tag="PLI69_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HOG41742.1"
                     /translation="MKRILFTLIAVLFFIPLQETNACTSAIITGKATPDGRPLLWKHR
                     DTGTLDNRIEYFDKSKDRNIKYAFIGLVDSPKETKEAWMGTNEVGFSIMNTASYNLND
                     EESPVQDQEGVVMYNALATCRTLADFEKMLSKMKFPTGVEANFGVIDAEGGAAYYEVS
                     SKDWIKVDANDPAIAPEGYLIYTNHSFTGKVNKGMGYIRYVSASDIFRDRFVKNQPMT
                     VQWIFNSLSRNFYHSLLDINLADNPEVAPNGWFIDQDFIPRKSTSASSVVKGVLPGEN
                     PELTVMWSIVGYPPVSVAVPLFVKSGKDLPAQVVARGENSEGLNPKNCEICDLAMARK
                     AGVFPVSRGNGGKYFRFDLLWNREGTGYIQRLAPYEEAIFDTFNPVLEKWYKKGSVNL
                     SELSELYSKMDIGAAVK"
     gene            complement(1632..2654)
                     /gene="ruvB"
                     /locus_tag="PLI69_04300"
     CDS             complement(1632..2654)
                     /gene="ruvB"
                     /locus_tag="PLI69_04300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOG41743.1"
                     /translation="MSKERFDPREESLSRERDIEGQIRPKDFEEFSGQDKVIENLKIF
                     VQAALLRGEALDHILLHGPPGLGKTTLAYIVANELGVNMKITSGPVLDKPGDLAGLLT
                     SLEEGDVLFIDEIHRLSPIVEEYLYSAMEDYTIDLMIDKGPGARSIQITLNPFTLIGA
                     TTRSGLLTSPLRARFGIKSHLEYYDAQVLKRIIIRSASILCIEINEDAAWEIALRSRG
                     TPRIANSLLRRVRDFAQVKGTGIIDLEIARIALNALNIDKRGLDAIDNKILTTIIEKF
                     KGGPVGAGTIATAISEDVGTLEEVYEPFLIKEGFLSRTPRGRVVTELAYKHLGMTIPG
                     RENLLF"
     gene            complement(3112..3897)
                     /locus_tag="PLI69_04305"
     CDS             complement(3112..3897)
                     /locus_tag="PLI69_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOG41744.1"
                     /translation="MDFTLKIWRQKNAKSKGHFETYKVKNISPDTSFLEMLDILNEQL
                     VNEGVDPVAVNKGCKDKKSDPVAFDHDCREGICGICSLYINGRAHGPDDEITTCQLYM
                     RKFKDGETITVEPWRTRGFPVIKDLVVDRGAFDKILQAGGYISVNAGNAPDANAILVQ
                     KEKANESMDAAACVGCGACVATCKNGSAMLFVASRVSSLALLPQGKIEGARRAKAMVA
                     KMDELGFGSCTNTRACELECPKGITIAHIARLNREFLSAKLKE"
     gene            complement(3916..>4141)
                     /locus_tag="PLI69_04310"
     CDS             complement(3916..>4141)
                     /locus_tag="PLI69_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOG41745.1"
                     /translation="GELMALDALNRRESCGGHFREEMQTEDGETMRDDENFMYVSAWE
                     YKGDDQEPTLHKEPLKFEFVKVASRSYAKI"
BASE COUNT         1163 a          926 c          814 g         1238 t
ORIGIN      
        1 tccagcagcc ggtcatttat aataaacgag ccggatccta ctttttcaga ccatgaagca
       61 tcggatgctc cctcaaacga gtttaaggct gataatgtgg ctgtacgctg tactttggca
      121 gtagcctctt tcagttcatt tttatcggtc ggtgatgggt aataatagat gcactcatca
      181 gaaaagaaac tataaaagtc gttggtaagg aaataggcat tcttcttggt atctgcaaat
      241 acgatatgca ctgatggatg ctccatcagg gagattaccg atgttatgta aaccgccttt
      301 gccgaggagg taaagcagtt gtctacataa ttccttggta tattttcggg aatatgcacc
      361 ttattttacc gcagcgccta tatccatttt tgagtagagt tcggagagtt ccgacagatt
      421 caccgaaccc tttttatacc acttttcgag tacgggattg aatgtatcga agatagcctc
      481 ctcgtatgga gcaagacgct gaatatatcc ggttccttct ctgttccaca gaagatcaaa
      541 acggaaatat ttacctccat ttcctctgga aacggggaat acacccgctt ttcgagccat
      601 tgcgagatca caaatctcac aattcttagg attgagacct tcgctgtttt cacctctggc
      661 cactacctgc gcaggaagat cttttccact ttttacaaat aggggaacgg caacacttac
      721 cggaggataa cctacaatac tccacataac agtcagctcg ggattctcac ccggaagcac
      781 accctttacc acactcgagg ccgaagtgga tttacgggga atgaaatcct gatctataaa
      841 ccatccgttg ggagctactt cgggattatc agcgaggttg atatcgagta gggagtgata
      901 aaaatttcgt gaaagggaat taaaaatcca ttgcactgtc ataggttggt tctttacgaa
      961 acgatcacgg aaaatatcac tcgcactcac ataacgtatg taacccatac ccttattcac
     1021 cttacccgta aaagagtgat tggtgtatat gagataacct tccggggcga ttgcgggatc
     1081 attggcatct acctttatcc aatctttgga gctcacttca tagtaagcgg ctccaccctc
     1141 ggcatcgatg acaccgaaat ttgcctccac tccggtagga aatttcattt tggagagcat
     1201 tttttcaaaa tcggcaagag tcctgcaagt ggcgagtgca ttgtacataa ccaccccttc
     1261 ctgatcctga acaggagatt cctcatcatt aagattgtat gaagcggtat tcataatgga
     1321 aaaacctact tcattggtac ccatccaggc ctctttggtc tccttgggac tatctactaa
     1381 ccctatgaaa gcatatttga tatttctatc cttggactta tcaaaatatt caatacgatt
     1441 gtcgagagta cccgtatcac gatgtttcca aagtaaagga cgtccgtcag gagtagcctt
     1501 tcccgtgatg attgccgagg tgcaggcatt tgtttcctgt aacgggataa aaaacaaaac
     1561 agctataagt gtaaaaagaa ttcttttcat tttgtattgt atctttattt ttagtttata
     1621 atccattaaa gtcagaagag tagattttcc cttccgggaa tagtcatgcc cagatgttta
     1681 taagcaagtt ccgtaactac cctgccgcgc ggagtacggg ataaaaatcc ctcttttatc
     1741 aaaaaaggtt cataaacctc ctcaagagtg cccacatctt cactaatggc tgtagctatc
     1801 gtaccggctc caaccggccc tcccttgaat ttttcaataa tggtggtaag aattttattg
     1861 tctatggcat ccaaacccct tttatctata ttgagcgcat tcaaggctat tcgcgctatt
     1921 tcaagatcta ttataccggt acctttaacc tgagcaaaat ctctaaccct tctgagcagt
     1981 gagttggcaa tacgtggagt accgcgactg cgcaaagcta tctcccatgc cgcatcttca
     2041 tttatttcta tacagagaat ggaggcactt cggatgataa tccttttgag tacctgcgca
     2101 tcataatatt ctagatgaga ttttataccg aacctggccc tcaaaggaga agtgagaagt
     2161 ccgctgcgag tggtagctcc tatcagcgta aatggattca gagtaatctg tatggaacgt
     2221 gctccgggcc ccttatctat catcaaatct atggtataat cctccattgc ggaatagaga
     2281 tactcctcca caataggcga aagacgatga atctcatcta taaatagcac gtcaccctct
     2341 tcaagggaag ttaaaagtcc cgccagatct ccgggcttat ccaaaaccgg accggaggtg
     2401 attttcatat ttaccccaag ttcattggcc acaatatagg caagagtcgt cttcccaaga
     2461 cccggaggac cgtgtaaaag tatatgatcc agagcctcgc ctctaagaag cgcggcctga
     2521 acaaaaatct taagattctc tatcacctta tcctgcccgg aaaactcctc aaaatctttc
     2581 ggacgtatct gtccctcgat gtcgcgttct ctggacaagg attcttccct cggatcaaat
     2641 ctttctttac tcatatctga cgtttgcgct cgaaagcgtt ttaaacatta caaaaataaa
     2701 taatattatt taaaaaagaa tagttgtatt tattagtgtg ttgataatgt ggataaaaat
     2761 attaggtaaa atatcaaaat catataaaca caatataaac atgaatatgg ttttatcgat
     2821 gttgtttaag attgttttgt tgatgttgtt tatagtttaa ttattattta taaacaagtg
     2881 tctcatgttg acaatgtttg taaaagaatt taaaaggaga tttgtagatt taataacaat
     2941 tgttatggta atatttttta taaaaagtta tcaaattgta tcaacactga atcaaccgtt
     3001 attagtagta gttattaaaa aaaaatggga tgaacccatt tttttaactg ttggccgttg
     3061 gccgttagct caatgtttgg tttttggctc ttagccttta tatgttggca tttattcttt
     3121 caacttggct gaaagaaact cacgattgag tctggcaata tgtgctatag tgataccttt
     3181 tggacactcc aattcacagg cacgggtatt ggtgcaacta ccaaaaccta gttcatccat
     3241 tttggcaacc atagcttttg cacgtctggc accttcaatc ttaccttgcg gcagaagagc
     3301 gagagaactt acacgagatg ccacaaatag cattgcagag ccatttttgc aagttgcaac
     3361 acaagcaccg caaccaacac aagcagctgc atccatactc tcattagctt tctctttctg
     3421 tacgaggata gcgttggcat cgggagcatt tccggcattt accgatatat atccacctgc
     3481 ctgaagtatc ttatcaaatg ctcccctgtc gaccacgaga tcctttataa ccggaaaacc
     3541 acgggtcctc caaggctcca cggttatggt ctcaccatcc ttgaatttgc gcatatagag
     3601 ctggcaggtg gttatctcat cgtccggacc atgtgcacgg ccattgatgt agagtgagca
     3661 gatgccgcag ataccttcac gacagtcgtg atcaaatgct acgggatcgg attttttatc
     3721 tttacaacct ttgttgactg caacagggtc tacaccctca ttgaccaatt gttcgttgag
     3781 gatatccaac atctcgagga aagaggtatc cggagagata ttttttactt tgtaggtctc
     3841 aaagtggccc ttacttttgg cgtttttttg acgccatatc tttaatgtaa aatccataac
     3901 ttttctcctt actcattata tcttcgcata gcttcgcgaa gcaactttaa caaattcaaa
     3961 tttcaaaggc tctttgtgaa gagtgggttc ctgatcgtcg cccttatatt cccatgcgct
     4021 tacatacatg aagttttcat cgtcacgcat ggtttctcca tcctcggttt gcatctcctc
     4081 gcggaaatga ccaccacagg attcacggcg attcagggca tccagtgcca taagttctcc
     4141 g
//
LOCUS       DAOSIE010000113         4123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_19107_length_4123_cov_3.056047, whole genome shotgun sequence.
ACCESSION   DAOSIE010000113 DAOSIE010000000
VERSION     DAOSIE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19107_length_4123_cov_3.056047"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLI69_04315"
     CDS             complement(<1..204)
                     /locus_tag="PLI69_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOG41746.1"
                     /translation="MVFTSENILLIGSIILFVSIFISSRVSAKIGIPMLLLFLILGMV
                     FGSDGIGIQFSDVKDAQFIGMFAL"
     gene            285..1484
                     /gene="hydF"
                     /locus_tag="PLI69_04320"
     CDS             285..1484
                     /gene="hydF"
                     /locus_tag="PLI69_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOG41747.1"
                     /translation="MSIRTIVIFGKRDTGKSSLINSLTGQQTAIVSDVPGTTTDPVRK
                     RVELPGIGISNIIDTAGLDDSGALGERRVAKSLEAMDLADLALILFTDNSFTSLERDL
                     AKTLNESLIPFVIVHNKSDIHPLDPELRAQLTEEYGNVIEYSCEVSDQDGAQSYRSAL
                     VDKIVRVLATIDDGEKPMLEGLVSASDNILLVCPIDEGAPHGRLILPQVTAIREVLDA
                     GAVATVLQPAALKKHLESGLLPDLVVTDSQFFAPVADIVPQKVPLTSFSVLLSRSKGP
                     FKALLEGVKVLGELKDGDRILMLESCTHHASCDDIGRVKIPALIRKVSGKKIDFDYIS
                     GLDPITTGGANKYRLAIQCGGCMVTTTQLRRRLARVMRDGIPVTNYGMTIAYLNGIIG
                     RAVAPLV"
     gene            complement(1601..2773)
                     /locus_tag="PLI69_04325"
     CDS             complement(1601..2773)
                     /locus_tag="PLI69_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG41748.1"
                     /translation="MKERLCNSNYIKVWSANFLLYFSFMLLTPLFPIYLSETFGADKQ
                     MIGLVLSGYTITALLVRPFSGYIVDSFPRKLVLVICFALVFVIFIGYPLSGSLLLFAI
                     VRTLHGAPFGAATVSNSTVAIDVLPASRRAEGIGYYGLSNNLASAISPTVALLIYGAT
                     QSYNVLFSHAIGISALALLCTMSVRLKDREKIVRPPISLDRFLLIKGWKLGLSMACFS
                     FAYGVLSTYIAIYGKEELGITSGTGLFFMLLSAGLIISRIAGGRALRDGKIMQNASTG
                     VLISLTGYLLFTLVHKEWSYYGAALIIGLGNGRMFPAFQTMFLNLTTNDRRGTANSTL
                     YVSWDLGIGIGILIGGIAAEHFGYNAAFVVMSAVNALGVLFYFVSAKGHFVKWRLR"
     gene            complement(2886..2959)
                     /locus_tag="PLI69_04330"
     tRNA            complement(2886..2959)
                     /locus_tag="PLI69_04330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2923..2925),aa:Arg,seq:tct)
     gene            complement(3049..3690)
                     /locus_tag="PLI69_04335"
     CDS             complement(3049..3690)
                     /locus_tag="PLI69_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOG41749.1"
                     /translation="MMLENFIKAIIVGLGAAIPLGPLGIMCIQKTISKGRNSGFSIGL
                     GSSLADVFYATIALFSLSFINDFIDQNRLWVIFIGGVIIFFIGLNIAVSNPVKQLRQP
                     KKSNGSKYAQEVLQGFAVTISNPGALVLLLGFFALVGIDIGEQYTRYSVVVILAGIFI
                     GTSLWWFILSTGINIFRRKFRLRQLLWINRISGTLIALLGLISAAEGLFRLIG"
     gene            3759..>4123
                     /locus_tag="PLI69_04340"
     CDS             3759..>4123
                     /locus_tag="PLI69_04340"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41750.1"
                     /translation="MKGEKKIRLKLLLLALLFGVLMSVPYLVPHCGLVALVSFIPLLY
                     MDKIASDNGIRRIWWYHYLAFLLFNIFSTFWIWFVSEVGAIAAIALNTLQMSIVFALY
                     RWSRRVFTYRWLSYLFLIIT"
BASE COUNT         1147 a          880 c          958 g         1138 t
ORIGIN      
        1 taacgcaaac attcctatga actgagcatc ctttacatcg gaaaactgta tgccgatacc
       61 gtcactgcca aaaaccattc ctaagataag gaagagcaac agcattggaa ttccaatttt
      121 ggcgctgaca cgactgctga taaaaatact tacaaacaga ataatggatc cgattagcag
      181 aatgttttcc gaggtaaata ccataatggg ttgcgtttgg ttataaaaat acaaaattac
      241 tcattttatt attaaatttg ccatcaaaac aaaaaataga acaaatgagt atccgtacaa
      301 tagtcatttt cggaaaaagg gatacaggta agagctcgct tataaactct cttacaggtc
      361 agcagacagc tattgtctca gacgttcctg gcacgactac cgatcctgtt cgtaaaaggg
      421 tcgaactccc cggtataggc attagtaata tcatagatac tgctggactt gacgatagtg
      481 gtgcccttgg ggagagaaga gtcgccaaga gcctcgaagc tatggatctt gccgacctgg
      541 cgttgattct cttcactgac aattcattca cctccctgga gcgtgatttg gcaaagactc
      601 ttaatgagtc attgataccc tttgtgatag tccataataa aagcgacatc catccgttgg
      661 atccggaact gcgggcacaa ctaactgaag agtatggtaa tgtgatagag tactcttgtg
      721 aggtgagcga tcaggatggt gcacagtcat accgctcagc tcttgtcgat aagattgtcc
      781 gtgtgcttgc aactattgat gatggtgaga aaccgatgct tgagggactt gtatcagcct
      841 cggacaatat actgttggta tgtcccatag atgaaggagc accccatgga cgactgattc
      901 ttcctcaggt aacggcaata agggaggtac tggatgcagg tgctgtggct accgtactcc
      961 agcctgcggc tcttaaaaaa catctggaat ccggattgtt gccggatctg gtggttaccg
     1021 acagtcagtt ttttgctccg gtagccgata ttgtgcctca gaaggtgccc ttgacctcgt
     1081 tcagcgtact cctctctcgt agtaaaggcc catttaaagc acttctagag ggtgttaagg
     1141 tacttgggga acttaaggat ggtgacagaa tccttatgct ggagtcctgt acccaccatg
     1201 cttcctgcga tgatatcgga agagttaaga ttcctgccct catacgtaag gtgagtggaa
     1261 agaagataga tttcgattat atttcgggat tggatcccat aacaaccgga ggggccaata
     1321 aatatcgcct tgcaatacag tgtggagggt gtatggttac aacaacacag ttgcgtcgtc
     1381 gtcttgccag agtaatgaga gatgggattc cggtgacaaa ctacggtatg acaattgcct
     1441 atctcaatgg aataatcggt agagccgttg ctccgctggt ttagtggtgc gggttacgag
     1501 gtgtatgttg cggtgtatgc gttactaggt atgcgaaagt actgagtgtg gtgtttgatg
     1561 ttctgatgtg taatggtagt aacgctacaa actttctgat ttatcttagc ctccatttta
     1621 caaagtgccc ttttgcgctc acgaagtaga aaaggactcc cagagcgttg actgcgctca
     1681 tcaccacaaa tgcggcattg tatccaaagt gctcggcggc gataccgcct ataagtattc
     1741 ctatgccaat gccaaggtcc catgagacat aaagagtgga gttggcagta cctctgcgat
     1801 cgttggtggt gagatttaga aacattgtct ggaaggcagg gaacattctt ccgttaccca
     1861 aacctataat cagtgctgct ccgtagtaac tccattcttt gtgtacaaga gtgaagagca
     1921 gatatccggt aagtgaaatc aacacacctg tggaggcgtt ttgcattatt tttccatctc
     1981 tgagagctcg cccgccggcg atacgtgata tgataagtcc tgccgaaagc aacataaaga
     2041 agaggcccgt gccgcttgta atgccgagtt cttctttgcc atagatggca atataggttg
     2101 agaggacgcc atacgcaaaa ctgaagcagg ccatagaaag gcccagtttc cagcctttga
     2161 ttagcaggaa gcggtcgagt gaaatgggtg gtcttactat cttctcgcga tctttgaggc
     2221 gtactgacat agtacagagt agcgcaagag ccgatatacc gattgcatgg ctgaaaagta
     2281 cattgtagct ctgcgtagct ccataaataa gcagggctac cgtaggactt atggcgctgg
     2341 ccagattgtt gctcagaccg taatacccta tgccttcagc tcttctggag gcaggcaaca
     2401 cgtctatggc cacagtgcta ttggaaactg ttgctgcacc gaaaggagct ccgtggagag
     2461 ttctgactat tgcgaagaga agtaaagaac cactcagagg atatccgata aaaatgacga
     2521 ataccagtgc aaaacagatt accagcacca gttttctggg gaaactatct acaatgtaac
     2581 cactgaatgg tctcacgagc agagcggtga tggtgtatcc cgagagaacc aagcctatca
     2641 tctgtttatc agcaccgaag gtttcgctca gatagatagg aaagagtggg gttagcagca
     2701 taaaggagaa atagagcagg aagttcgcac tccacacttt gatataatta ctgttgcata
     2761 gtctctcttt catacgggtc ggggcgatct atccttcttt attgctattt ttcgtaaaac
     2821 ttgtacaaaa acgagccaga tactaacaaa aaccccttct atattatata cagaagggat
     2881 tctcgtgatc cggttgggat tcgaacccaa gacccacagc ttagaaggct gttgctctat
     2941 ccaactgagc taccggaccg gttccttgtc tcatttgagg gtgcaaatgt attgtttttt
     3001 ttttggagaa gcaaagaaaa gtgacaattt ggtatacatc ccatattttc agcctataag
     3061 tctgaacaat ccttcagcag ctgaaataag tcccagcaga gctattaatg taccggatat
     3121 gcgattgatc cacaataatt gacgaagtcg gaacttccgt ctgaaaatat tgatacctgt
     3181 actaagaata aaccaccata gggaagttcc tataaaaata cctgccagta tcactaccac
     3241 gctatatctg gtatattgct ctccaatatc aatccccacc aaggcaaaaa agccaagcaa
     3301 tagcaccaga gctccgggat ttgagatagt tacggcaaac ccttgcaata cctcctgagc
     3361 atactttgat ccattactct tcttgggttg ccttagttgc ttgacgggat tgctgacagc
     3421 aatgttaagg cctatgaaga aaattatcac tccacctatg aagattaccc aaaggcggtt
     3481 ttggtctatg aaatcgttta tgaaagagag tgagaagagg gcaattgttg cgtagaaaac
     3541 atctgccagc gaagatccga ggccaatgga aaaaccggag tttcgtcctt tgcttatggt
     3601 cttctggatg cacatgatac ccagaggtcc cagaggaatt gcagctccca gtcccactat
     3661 gatcgctttt atgaagttct ctaacatcat tttatttttt tgcaaacgca aatatagtca
     3721 acttgctcta tttgttcaaa aaaactatct ttgcatgtgt gaaaggggaa aagaaaataa
     3781 gacttaaatt gttgttgctc gcactccttt tcggagtcct gatgtcagta ccatatctcg
     3841 tgccacattg tggccttgtg gcgctggtga gtttcattcc acttctctat atggataaga
     3901 ttgcatcgga caacgggatc cgtcgtattt ggtggtatca ttatcttgca tttttgctat
     3961 tcaatatatt ttcgactttc tggatctggt ttgtttccga agtgggtgca attgctgcaa
     4021 ttgcactcaa tacgttacag atgtccatag tttttgccct gtatcgttgg agtcgcaggg
     4081 tatttactta ccgttggctc tcttatctgt tccttataat cac
//
LOCUS       DAOSIE010000114         4098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_19237_length_4098_cov_11.625773, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000114 DAOSIE010000000
VERSION     DAOSIE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19237_length_4098_cov_11.625773"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PLI69_04345"
     CDS             <1..799
                     /locus_tag="PLI69_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG41751.1"
                     /translation="AITFKLIPLWNEHMPTGASKIKKRTFPEKELKVVYFPSCITRSM
                     GTSQSYGKEKEITRMTEALLTAAGYQIIYPENMNNLCCGMLFSSKGYVETGKKASDEL
                     KAALIKASENGKYPVLCDMSPCLYTMRTNMEGELKLYEPAEFIDKYLLDRLELKPVDE
                     KVAIFAVCSAKQMEVESNLENVARRCAREVVIVDSNCCGFAGDRGFFFPELNQHGLRD
                     LEKQSEGCSHGYATSRTCEIGLSRHSGIEFKSIIYLAAQAAGIDTSK"
     gene            811..2433
                     /locus_tag="PLI69_04350"
     CDS             811..2433
                     /locus_tag="PLI69_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOG41752.1"
                     /translation="MFGSLSDYVKFLESERELIRIKEFVDPVLEIAEVTDRVSKMPGG
                     GKALLFENTGTRYPVLTNMMGSEKRMLYTLGAASFEEIEQRFEDIIHRVLKEKSTLMD
                     KLKMLPLLKEASRWLPVNHRGSAPCQEVVKYNAKLSDLPILKCWPQDGGRFITLPLVH
                     TVSPVTGQRNVGMYRMQVFNDNTTGMHWHKHKTGAKHFMESGHRLPVAVCLGGDPIYT
                     YCATAPLPDGIDEYLLAGFLRDKPVELVECFTQKLKVPADCDFVIEGYVQKSEPLVIE
                     GPFGDHTGFYSLADYYPQFHVTCISHRKDAIYPATVVGVPPQEDKYMAMATEKIFLAP
                     IKFVISPEIEDLYLPEEGVGHNFAVVKINKSYDGQAFKVANALWGAGQMMFNKFLIVV
                     DDDIDIRDHNALKESIGKHYFPETDTLVSKGPLDILDHSGPRVGFGGKLCIDATAKEY
                     SRKEPVEIGQIIKVTFDKDEEQSSEYRKLWLLGANCDAERDSSIKKGVLHLDATSKKG
                     MKGFDREWPDIVTSSEETKAAVDAKWASLGIE"
     gene            complement(2805..3653)
                     /locus_tag="PLI69_04355"
     CDS             complement(2805..3653)
                     /locus_tag="PLI69_04355"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOG41753.1"
                     /translation="MTLLQEEETVTSTCAYFGITRQAYYKSRKHIENKAFEEEIVVEL
                     VQEKRQIMPRLGGRKLHYLLKDDLSKIGKIGRDKFFDILGQNDLLVKPKKSFTRTTYS
                     FHHYYKWKNLIKDLLVTRVNQVWVSDITHIRTLNGFAFLFLITDLYSRKIVGWVLSNN
                     LGVEGALKALKMAFASRDDKSESLIHHSDRGIQYCCKEYVKTLQEAGIDISMTEENHC
                     YENAVAERVNGILKNEFYLDATFNNFEQALSAVVSGIKNYNENRPHWALELQTPSQVY
                     YKTKVA"
     gene            complement(3623..4054)
                     /locus_tag="PLI69_04360"
     CDS             complement(3623..4054)
                     /locus_tag="PLI69_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG41754.1"
                     /translation="MTKVQRTTTRYSDCFKLQVVEEIEKNGLTIDECRLKYGIGGGST
                     IQRWVRKYGKQHLLNKIVRVETRDEIDELKRLRAENKALKEAYADLSLQHKDLSLQYA
                     ELSVNHKCSEKVIEVADELYDMDLKKKYEQELSHYFKKKKR"
BASE COUNT         1147 a          864 c          907 g         1180 t
ORIGIN      
        1 agccatcacc ttcaaattga ttccgttgtg gaatgaacat atgcctacag gtgcaagtaa
       61 gattaaaaag agaacttttc ccgaaaagga gctcaaagtg gtctatttcc cctcatgtat
      121 taccagaagc atgggtacat cgcagagtta tggcaaggag aaagagatta cacgcatgac
      181 tgaagctctg ctcaccgctg caggatacca gattatatat cccgaaaaca tgaacaatct
      241 ttgctgcgga atgctcttct ctagcaaggg ttatgtagaa acaggtaaaa aggcctcaga
      301 cgagcttaag gctgctctta ttaaagcctc cgaaaatggg aagtatcccg ttttgtgtga
      361 catgagtccc tgcctctata ctatgcgcac aaatatggag ggtgaactta aactatatga
      421 gccggcagaa tttatagaca aatatctgct tgaccgtctc gaattgaaac ccgtagatga
      481 aaaggtagcg atttttgcag tatgttcagc aaagcagatg gaagtggagt ccaatctgga
      541 gaatgtggcc cgaagatgtg cccgtgaggt ggtgattgtg gactccaatt gctgtggttt
      601 tgcaggtgac agaggatttt tcttccccga gctcaatcag cacggattgc gagacctgga
      661 aaaacagagt gaaggttgca gtcacggcta cgctaccagc cggacttgtg aaataggtct
      721 ttcgcgtcat agtggcatag aattcaaatc aattatttat cttgcggcac aagcagcagg
      781 tatagataca agtaaataaa atataagaat atgttcggca gtctctctga ttatgtaaag
      841 tttctggaat ccgaaaggga acttatccgc atcaaggaat ttgtggatcc cgttttggag
      901 attgctgaag tgacagaccg cgtcagcaaa atgccggggg gcggtaaggc tctactcttt
      961 gaaaatacag gcaccagata tcccgttctg accaatatga tgggctcgga gaaacggatg
     1021 ctctacactc tcggagctgc ttcatttgaa gagatagagc aaagatttga agatataatc
     1081 caccgggtac taaaagagaa gagtactttg atggataaac taaagatgct tcctctacta
     1141 aaagaggcct cccgatggct tcccgtcaac cacagaggat cagctccctg ccaggaggtg
     1201 gtcaagtaca atgccaagct tagcgacctt cccatattga aatgctggcc gcaagatgga
     1261 ggcaggttta tcacacttcc tctcgtgcac accgtcagcc cggttaccgg acagcgcaat
     1321 gtaggtatgt acagaatgca ggtatttaat gacaatacca ccggaatgca ctggcacaaa
     1381 cacaaaaccg gggcaaaaca ttttatggag tccggtcaca ggctccctgt ggctgtttgt
     1441 cttggaggag atccaatcta tacctattgc gctactgctc cccttcctga tgggattgac
     1501 gaatatctgc ttgccggatt cctacgggat aagcctgtag aattggtgga gtgttttacc
     1561 cagaagctca aggtacctgc tgattgcgat tttgtgattg agggatatgt gcagaaaagc
     1621 gaaccgctgg ttattgaggg gcctttcggc gaccacacag ggttctattc gcttgcggat
     1681 tattatccgc aattccacgt tacctgcatt tctcaccgca aggatgccat ctatcccgcc
     1741 acggttgtag gggtacctcc ccaggaggat aaatatatgg ccatggccac cgaaaagata
     1801 tttttggctc ccatcaaatt tgtgatttct ccggaaatag aagatctcta cctgcctgag
     1861 gagggtgtag ggcacaattt tgctgttgta aaaataaaca agagttacga tggtcaggcc
     1921 tttaaggtag ccaatgcgtt gtggggagcc ggacagatga tgttcaataa attcctgatt
     1981 gtagtggatg atgatataga tatccgggac cataatgcac tcaaggagtc tataggcaag
     2041 cattattttc ccgaaaccga tactctggtg agcaaagggc cattggatat actcgaccat
     2101 agcgggccaa gggtgggatt tggtggtaaa ttatgcattg acgcgactgc caaggaatat
     2161 agtcgtaaag aaccggttga aataggtcag ataataaagg taacctttga taaagatgaa
     2221 gagcaaagta gtgagtatcg taaattgtgg ctgctcggag ccaactgtga tgcggagcgc
     2281 gactcttcca tcaaaaaggg agtcctgcat ctggatgcca cctccaaaaa gggaatgaaa
     2341 ggatttgacc gcgagtggcc cgacatcgtt acctcctccg aggagacaaa agcagctgtg
     2401 gatgccaaat gggcatccct ggggattgag tgaataatga cgagatgcac attgcgagat
     2461 gtgaaatgct aaattcttta gcaaaatata gacaacggcc aacaactaat ggctaatggc
     2521 caacagccaa tagctaacgg ctctcctccg ccacccactg tctcgtcctg aaaaaagttg
     2581 acacattttg ttaaacttta ctgttgtcat tatttctttt ttcctttttg aaattgatag
     2641 ctatttgtca agttagaaag ataaattcag tcaaggtttt gcgaaaaaat actatccgtc
     2701 aggatggaga tttttccggc aaacccgtag ggcttgacct ttacttaatt tagctttcga
     2761 cttatatttg cgaccaattt aaaaaacatt tttctaattt cattctatgc aacttttgtc
     2821 ttataataaa cttgagacgg agtttgcagt tccaatgccc agtgagggcg gttttcatta
     2881 taattcttta ttccggatac tacggccgaa agggcttgtt caaagttgtt aaacgtggca
     2941 tccagataaa attcattctt caatatgccg ttcacacgct ctgcaacggc attttcatag
     3001 caatgattct cttcggtcat actgatgtca atccctgctt cttgaagtgt ttttacatat
     3061 tctttacaac aatattgtat gcctctgtca gagtgatgta tcaacgattc ggacttgtcg
     3121 tcacgagatg caaaggccat ctttaaggct ttcagagcac cttcgacacc caagttgtta
     3181 ctcagtaccc agcctactat cttcctggaa tacaagtcgg taattaaaaa gagaaaggca
     3241 aagccattaa gtgttcgtat gtgcgttatg tcgcttaccc acacttgatt aacccgggtt
     3301 acgagcaaat cctttatcaa gtttttccac ttgtaatagt gatggaaaga ataggttgtc
     3361 ctggtgaaac tcttcttggg tttcacaagc aaatcgtttt gaccgagtat atcaaagaac
     3421 ttatcccttc cgatcttgcc tattttagac aaatcatcct ttagcaaata gtggagtttt
     3481 cttccaccca aacggggcat aatctgcctc ttttcctgaa ccaactcaac gactatttct
     3541 tcctcaaatg ctttgttttc aatatgtttt ctgctcttat aataggcttg acgggttatt
     3601 ccaaagtaag cacaagttga cgttaccgtt tcttcttctt gaagtaatgt gataactctt
     3661 gttcgtactt tttttttaga tccatgtcat aaagctcatc ggctacctct ataacctttt
     3721 cagagcattt gtgattcacg gaaagctctg cgtattgtag ggacagatct ttgtgttgta
     3781 aagaaagatc tgcataagct tctttcaagg ctttgttttc tgctcgaagg cgttttagtt
     3841 cgtctatttc gtctctggtt tccacgcgga ctattttatt taataagtgc tgtttaccat
     3901 atttccgaac ccatctttga atggtcgagc caccaccgat gccatatttt aaacgacatt
     3961 catcaatagt taaaccattc ttttcaattt cttctactac ttgtaactta aagcaatcgc
     4021 tgtaccgtgt tgttgttctc tgaacttttg tcataatttt tcctgattaa agtgtaaacc
     4081 tatttcagga catgacac
//
LOCUS       DAOSIE010000115         4087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_19289_length_4087_cov_4.024058, whole genome shotgun sequence.
ACCESSION   DAOSIE010000115 DAOSIE010000000
VERSION     DAOSIE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19289_length_4087_cov_4.024058"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLI69_04365"
     CDS             <1..225
                     /locus_tag="PLI69_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOG41755.1"
                     /translation="ILNMISTSVMIRLGHVKGNKMVDMQLTNAKLVDRGTRMIMDEAK
                     LDDYEFAQSLLLKHGSVRKAVDAYLATKQG"
     gene            225..839
                     /gene="recR"
                     /locus_tag="PLI69_04370"
     CDS             225..839
                     /gene="recR"
                     /locus_tag="PLI69_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOG41756.1"
                     /translation="MYKSNYSSKLLDDAVEQFASLPGIGRKSALRLALHLLRQPLENV
                     ERFSESFISLKSQIRYCPVCRMISDDGVCPYCSDSRRDRSIVCVVESLRDVISIENTG
                     SYNGLYHILGGIISPMDGVGPGDLAIAPLIERIREHDIKEVVLALSTGMEGETTSFYI
                     YKQISSLPVKVTAIARGVGFGDDLEYTDELTLGRSIEARQIFKP"
     gene            855..2378
                     /locus_tag="PLI69_04375"
     CDS             855..2378
                     /locus_tag="PLI69_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOG41757.1"
                     /translation="MRHEFPFALFLVAFAAYTLLLFIISWVTGRKAGSSSFFSGDRRA
                     PWPIVAYGMIGASISGVTFISVPGNVWVQNFYYMPLVLGFVVGFIVVARVLLPLYYKM
                     NLTSIYTYLGDRFGYRSHKTGTVVFMVSRVLGAALRIFVVIVVFFTFIPRSMVDATSP
                     LTVFILIAAIFLILLYFYTFKGGVKTIIWTDVLQTTFMLIAVGMTIYYIGKNMGLTFG
                     QLFSNLSGTINTNAGTVGYGRPFISWFDWDWSHGTNAVKQFISGIFVTIAMTGLDQAM
                     MQKNLACKDIKAAQKNMYTTSITIAVVNYFFVLMGALLCVYAQHLGGFEAIGITKTDE
                     LFPVIASQYFGIGAGVFFLIGLISASYPSAGAAMTSLTTSFCVDILKFNTRTDLSESR
                     KEKVRKLVHAGVGFVFLVIIVVLYLVSNDAVINLLYKLASYTYGPLLGLFFFGILTKY
                     KVRDRAVPWIAIAAPVICFIVNTLGKKFFGFDLGFTLLIVNGLLTFFGMWLFRVKTR"
     gene            2397..3158
                     /locus_tag="PLI69_04380"
     CDS             2397..3158
                     /locus_tag="PLI69_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOG41758.1"
                     /translation="MVRISAIKYLNTIPFIYGLENSPIMDEIVMDFCTPAESADNLIH
                     DRSDVGIVPVAIIPSLPDPHVISDYCIGATDNVASVLLCSGLPLNEISHIKMDGDSRT
                     SQLLTKVLCRDYWKIFPEFVPFNYEQEGIDPAGSYLLIGDKALIEAERFKYTYDLARE
                     WIDFTGKPFVFACWTANKPLGEEFVKKFNEALRYGIENIRGVIKKHCYQFPEEYAETY
                     LKSNISFVLDSDKRAGLTEFWRVALEKLNPRVHWL"
     gene            3204..>4087
                     /locus_tag="PLI69_04385"
     CDS             3204..>4087
                     /locus_tag="PLI69_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOG41759.1"
                     /translation="MIGFFYKSNDQIATSDNTEILKSILQKDVLWIDLFDPSGDEKRS
                     VEHFLNTNLQSRAQAEEIESSSRYSETQDNIFINTDFLIPGPENYNMESVSFILTGDT
                     ITSIRQVPLRSFTDIQRRIMANPRIYPTGYHILIGILENRIDLDADMIELMSKEIAQY
                     SKRVSLGEDVGEEFLLDINQLQENNMMVRENIVDKQRMLSSILKSEKSPDGITNKLNI
                     LLKDVSSLINHTNFSFERLEYLQNTVLGLINLEQNKIMKIFTLVSLLLMPPTLIASIY
                     GMNIKLPLIGGLWDILLLG"
BASE COUNT         1063 a          859 c          923 g         1242 t
ORIGIN      
        1 atcctaaata tgatttcgac ctccgtaatg attcgtttag gtcatgtaaa aggcaataag
       61 atggtagata tgcagctcac caatgctaag ttggtagatc gcggcacgag gatgatcatg
      121 gatgaggcga agctggatga ttacgagttt gcacaatcac ttcttcttaa acatggttct
      181 gttaggaagg ctgttgatgc ctaccttgct acaaaacagg gataatgtat aagagcaact
      241 attcatccaa gctcctggac gatgcggtag agcagtttgc ctcccttccc ggtataggtc
      301 gcaaaagtgc cctaaggctg gcattgcacc tgcttagaca gcctctggag aatgttgaac
      361 gcttttccga atcctttatc tccctcaaaa gtcagatacg ttactgtccg gtgtgccgga
      421 tgatatctga cgatggtgtt tgcccttatt gctcggatag ccgtcgcgac cgctccatcg
      481 tatgcgtggt ggagtcgctt cgtgatgtca tcagtataga aaacacaggc tcttataacg
      541 gtctttatca tattttggga ggtatcatct ctccgatgga tggtgtagga ccgggagacc
      601 tggcgatcgc tccactaatt gagaggatcc gtgaacatga cataaaagaa gtggtgctgg
      661 ctcttagtac aggtatggag ggtgagacca cttcttttta tatatacaaa cagatatctt
      721 ctttgccggt aaaagttacc gccattgccc gtggagtggg atttggggac gaccttgagt
      781 acacggacga gctgaccttg ggacgttcca tagaggcccg gcagattttc aaaccctaat
      841 tattttggat gattatgaga cacgaatttc cttttgcgct ctttctcgtg gcctttgccg
      901 catatacttt attgttgttt atcatctcct gggtgaccgg caggaaagcg ggctcaagtt
      961 cctttttctc cggtgacaga agggctccct ggccaattgt ggcctatggt atgattggtg
     1021 cctccatttc aggagttaca ttcatttcag ttccgggcaa tgtctgggtg cagaactttt
     1081 attacatgcc actggtactg ggttttgtag tgggttttat tgtagtagcg agggttttgc
     1141 ttccccttta ttataagatg aatcttacct ccatatatac ctacctgggg gatagatttg
     1201 gttacaggtc tcataagacc ggtactgtag tctttatggt ctctcgtgtt ctgggagcag
     1261 cattgaggat ttttgttgta atcgtagtct tcttcacctt cattcctcgt agcatggtgg
     1321 atgcaacttc acctctgacg gtattcattc tcatagctgc cattttcctt atccttctct
     1381 atttttacac cttcaagggt ggggtaaaga ccattatctg gaccgatgtg ttgcagacta
     1441 catttatgtt gattgcagtc ggtatgacaa tttattacat aggcaaaaat atgggtttga
     1501 ctttcggcca gttgttctca aatttgagtg gtaccataaa taccaacgcc ggtacggtag
     1561 gctacggcag acctttcata tcctggtttg actgggactg gagtcacgga actaatgccg
     1621 taaaacagtt catatccggt atttttgtga caattgcaat gacaggtctg gatcaggcca
     1681 tgatgcagaa aaatcttgct tgcaaggata tcaaggcagc acaaaagaat atgtacacaa
     1741 catccatcac aattgccgta gtaaactact tcttcgtgct catgggtgcc ctgctctgtg
     1801 tttatgccca gcatctggga ggttttgagg caattggaat caccaaaacg gatgaacttt
     1861 ttcctgtaat tgcgagtcag tattttggta taggagccgg tgtgtttttt cttatcggtc
     1921 tgatctcagc atcctaccct agtgcgggag cggctatgac atcactaaca acctcttttt
     1981 gcgtagatat tctcaaattt aacacccgta ccgacctttc ggagtcaaga aaggagaagg
     2041 tacgcaaatt ggtacacgcg ggggtgggat ttgtcttcct tgtaatcata gtcgtactct
     2101 atcttgtaag caacgatgcc gtcatcaatc ttctttacaa acttgcatca tatacttacg
     2161 gtcctctttt gggtctcttt ttcttcggta ttcttaccaa atataaggtt cgtgaccgtg
     2221 cagtaccctg gattgcaatt gcggcccctg tgatatgctt tatagtcaat actctgggaa
     2281 agaagttctt cggatttgat ctgggcttta cgcttctgat tgtcaatggt ctgcttactt
     2341 tcttcggaat gtggcttttc agagttaaaa caagataaat ttattatatt tgtaagatgg
     2401 tcagaatctc agcaataaaa tatctcaata caattccttt catctatggt ctggaaaatt
     2461 ctcccatcat ggatgagatt gtaatggatt tctgcacccc tgcagagtcg gctgacaatc
     2521 tgattcatga tcgtagtgac gtaggcattg ttcccgtagc gataattcct tcgcttcctg
     2581 atccgcacgt catctctgat tactgtatag gtgccacgga taatgttgca agcgtactgc
     2641 tctgttcggg tcttccttta aatgagataa gtcacatcaa gatggatgga gactcccgta
     2701 catcacaact tttgaccaag gtgctttgtc gtgattactg gaaaatattt cctgaatttg
     2761 tcccgtttaa ctacgaacag gaaggaatag atcctgccgg gagttatctt ttgataggag
     2821 acaaagctct gattgaagca gagcgtttca aatataccta cgatcttgcc cgtgaatgga
     2881 ttgacttcac aggtaaacca ttcgtatttg cctgctggac ggcaaacaag cctcttggag
     2941 aggagtttgt aaaaaaattc aatgaggcac tccgttatgg gattgaaaat atcagaggtg
     3001 tcataaaaaa acattgttat cagtttcctg aagagtacgc tgagacctat ctcaagtcaa
     3061 acatttcgtt tgtacttgat agcgacaagc gtgccggcct tactgagttt tggagggtcg
     3121 ctttggagaa actgaacccc cgagtccatt ggttatagcc ccgttttgtt cacggaaaat
     3181 ccaccagcca aaaacttgag acaatgattg gattttttta caaatcaaat gaccagatag
     3241 caacatctga taatacggaa atcttaaaga gtatcctcca aaaagatgtt ctctggatcg
     3301 atctttttga cccatccgga gatgaaaaac gttctgtaga gcattttcta aacaccaacc
     3361 tgcagagccg ggcgcaggcg gaagagattg agagttcatc ccgctattcc gagactcagg
     3421 ataatatatt catcaatacc gactttctga ttccgggccc tgagaactac aatatggagt
     3481 ctgtgtcgtt catcttaacg ggcgatacca tcacctccat acgtcaggtc cctttacgaa
     3541 gttttactga tatccagagg cggataatgg ccaaccctcg catttaccct acgggttacc
     3601 acattttgat aggtatactt gaaaacagga tagacctcga tgcagatatg attgagctga
     3661 tgtcgaaaga gattgcgcag tatagcaaaa gggtgagtct gggtgaagat gttggggaag
     3721 aattcctttt ggatattaac cagttgcagg aaaataacat gatggtacgt gagaatatcg
     3781 tggataaaca gcgtatgctc tccagtattc ttaaaagcga aaaatccccc gatggcataa
     3841 ccaacaaact gaatattctt cttaaagacg tctcttcgct gatcaatcat accaacttca
     3901 gttttgaacg tctcgaatat ctgcaaaata ccgtactcgg tctcatcaac cttgagcaga
     3961 acaaaatcat gaaaattttt actttggtgt cgttactgct tatgcctcct actcttatag
     4021 cgagcattta tggtatgaat atcaaattgc cgttaatagg cggtctttgg gatattttac
     4081 ttctggg
//
LOCUS       DAOSIE010000116         4055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_19492_length_4055_cov_3.532500, whole genome shotgun sequence.
ACCESSION   DAOSIE010000116 DAOSIE010000000
VERSION     DAOSIE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19492_length_4055_cov_3.532500"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PLI69_04390"
     CDS             complement(<1..687)
                     /locus_tag="PLI69_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41760.1"
                     /translation="MNIIGTGRAHPSLVVTNEMLSTFLDTSDEWITTRTGIKQRQLIS
                     HEKLEDLAAEACNKALEDAGITAKDLDFIICSNVVNEYITPSLSCIVQGMVGATCPCL
                     DLNSACAGFIYALDIANSFFRSKSDVNHILVLCAEEPTRMCDWQERSTCVLFGDGAGA
                     AVLSRGDDFKSFSLSSFSNIDPLYQIRQLEKTPFITKEEFGGPLVMKGQDVFKLAVNS
                     SLNDIRRVVEY"
     gene            853..1002
                     /locus_tag="PLI69_04395"
     CDS             853..1002
                     /locus_tag="PLI69_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41761.1"
                     /translation="MNKILPDYLLEKRHQIVTVIVTIVISVGFLCYISRISINICAFF
                     IYNLV"
     gene            1085..1897
                     /locus_tag="PLI69_04400"
     CDS             1085..1897
                     /locus_tag="PLI69_04400"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOG41762.1"
                     /translation="MCRLRKRRQFRSWSYALWMLAEALVITLFYSYVTHTLMKADLII
                     GNFPFVTLFSKGLVVVIAILILSNTISILWSIQRDQKERLRILYAGWRRNAHSPLSVD
                     GKPISAGAGISAGDFAAKRKSSVESEDDDDMIGSIHQDIDPRIINLYDHNGIMRFSIK
                     ESDLYYIISQDNYVRIHYLNNGEMSSYMLRCPLKVIEKSLKGSSLIRCHRSYMVNISR
                     IKVLRSEREGFFIDLDYDGAAEIPISKSYSDTYMEHFSKKVAGKELDDINIK"
     gene            1921..2097
                     /locus_tag="PLI69_04405"
     CDS             1921..2097
                     /locus_tag="PLI69_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOG41763.1"
                     /translation="MEKQAILKAMQEADKPLGAGDVAKLTGIDRKVVDKVFAEMKKSG
                     EIISPVRCKWTPAK"
     gene            complement(2232..3431)
                     /locus_tag="PLI69_04410"
     CDS             complement(2232..3431)
                     /locus_tag="PLI69_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG41764.1"
                     /translation="MLTLSEKAQKMPASPIRKLVPFADAAKKRGVKVYHLNIGQPDIA
                     SPKEALDAVKNNQLKLVEYPNSAGNKSLLEGLVKYYKGINIDIDTSCINITNGGSEAL
                     QIALAVTCNPDDEVIVFEPFYTNYNSFALQNDVILKPITTKIEDGFALPPMSEVEKKI
                     TPKTKGIIICNPGNPTGSLYTKEALMELGAIAKKYGLYIYSDEVYREFCYTDEPHFSC
                     MHIPGLEENVILLDSVSKRYSLCGVRIGFIVSKNKEVMKYVLRYAQARLCSPAYGQIA
                     AEGALDTPAEYFKEVRDEYIHRRNVLIEGLRKIKGVQVPMPMGAFYAVVQLPVDDSDK
                     FAQWLLEEFEYEGQTVMVAPLAGFYATKGLGTNQIRMAYVLKEEDLKAALKCLEVALD
                     KYPGRVK"
     gene            complement(3485..>4055)
                     /locus_tag="PLI69_04415"
     CDS             complement(3485..>4055)
                     /locus_tag="PLI69_04415"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOG41765.1"
                     /translation="LTYLAETFSPELIEIINVTNTTSNNFYAETMLKMLGKVLVDNSY
                     FYASTEAVEQYLRDHGVDTYGLCQDDGSGLSRENYVSPRFFTNFYTYKEQSATFAQYF
                     ESFPGPGRPGTLEGVLKEEKKEIKEIIRAKSGSLSSVRCYAGYVKTKKGYIKFAILVN
                     NFDCPTSAVQPKIEGFMRELAKYGNGNHR"
BASE COUNT         1127 a          879 c          874 g         1175 t
ORIGIN      
        1 atattctact actctgcgaa tatcattcag cgaagagttc actgcaagtt taaagacatc
       61 ttgtcccttc attacgagcg gacctccgaa ttcttccttt gtgatgaaag gagtcttttc
      121 gagctgcctg atctgatata gggggtcaat gttgctaaat gaagagagag aaaaggattt
      181 gaagtcatct cctctggaga gaactgctgc acctgcacca tctccgaaaa ggacgcatgt
      241 gctgcgctcc tgccaatcac acatacgtgt tggctcctct gcacaaagca cgaggatatg
      301 gtttacgtca ctcttgcttc tgaaaaaaga gtttgcgata tccaaagcat aaataaaacc
      361 cgcacaggca ctgttcaggt caagacaagg gcaggtggct cctaccatac cctgaacaat
      421 acaactcaat gagggagtaa tgtactcatt tactacatta gagcaaatta tgaaatccag
      481 atcctttgcg gtgattccgg catcttccaa tgctttattg caagcttccg cagccagatc
      541 ctctaacttt tcgtgtgaaa taagttggcg ttgctttata ccggtgcgag tggtaatcca
      601 ctcgtcactt gtgtcaagaa aagtggagag catctcattg gtcactacaa gtgacggatg
      661 ggccctcccc gtcccgataa tattcattct tttaaaaatt tttgagtttc gcccgcaaag
      721 ctattttagt ggcgacaaat gacaaaaaga ttgtggttgt cgatgatgtt atgtggtaga
      781 attcgtccct tttggggtaa aattttcgtt tttgtggcat cttttattaa ctttgaatat
      841 taattagaca atatgaataa gatattaccg gattatctgc ttgaaaaaag gcaccagata
      901 gttaccgtca ttgtcactat tgttatatca gtaggatttc tatgttatat cagtaggatt
      961 tctatcaata tatgtgcctt tttcatctac aatctggttt aaactatcag gggcagtttc
     1021 gttctttctc acggtaggat gcgtagcagt tgcttttctg attcttgccg cgtgtcgtgt
     1081 acttatgtgc agactaagaa aaagacggca attccgctct tggagttatg ctctttggat
     1141 gcttgccgaa gcacttgtga taactctctt ttattcctac gtgacgcaca cgctgatgaa
     1201 agcggacctt ataataggaa atttcccttt tgtgaccctg ttttccaaag gtctggtagt
     1261 ggtaattgcc atacttatcc tttcaaacac catctccata ctctggtcta tccaacgcga
     1321 ccaaaaggag cgtttgagga tactttatgc cggatggaga aggaatgcgc atagtcctct
     1381 aagcgtggat ggcaaaccta tctctgccgg agccggaatc agtgcaggtg actttgctgc
     1441 aaagagaaaa agtagcgtcg aatcggaaga cgatgatgat atgatcggct caattcatca
     1501 ggacattgat cccagaatta tcaaccttta tgatcacaat ggcattatga ggttttccat
     1561 aaaagagtcc gacctctact atataatatc ccaggacaac tacgtgcgta tacactacct
     1621 aaacaatggc gaaatgtcga gttatatgct gcgttgcccc ctcaaagtga tagaaaaatc
     1681 tctcaagggc tcttcactga tcagatgtca ccgctcttat atggtcaata tatcccgaat
     1741 aaaggtacta cgcagtgaaa gagaaggttt tttcattgac ctcgattatg atggtgctgc
     1801 agaaattcct atctcaaaga gctattccga tacctatatg gaacatttct cgaaaaaggt
     1861 agcgggcaaa gaattagacg atataaacat caaataacta taaataaata tttttgaatt
     1921 atggaaaaac aggctatact aaaagctatg caagaggcgg acaaacccct tggagcaggt
     1981 gatgttgcaa aactgacagg aattgaccgt aaagtagttg acaaagtatt tgcagaaatg
     2041 aagaaaagtg gagaaataat ctctcctgtc agatgcaaat ggacaccggc gaaataagta
     2101 ccggggtgca gggtgcgggg tgcaggttat gcgctactcg taactgactc ataacaaaaa
     2161 agagggtgac atcaagtcga tttgactttg ttgtcaccct cctcatttgg aagcctatat
     2221 tccgcaaaaa gttatttcac tctgccggga tacttatcaa gagccacctc gaggcacttc
     2281 agtgcagcct tgaggtcctc ctcctttagc acgtatgcca tacggatctg attggtacca
     2341 agtcccttgg tggcatagaa tcctgcaaga ggggctacca ttactgtctg accttcgtac
     2401 tcaaactcct caagcaacca ttgcgcaaac ttgtcactgt cgtctacagg tagttgcacc
     2461 acagcataga atgcacccat aggcatggga acctgaacac ccttaatctt cctaagacct
     2521 tcgattagca cattgcgacg atgtatatac tcgtcacgaa cttccttgaa atattctgca
     2581 ggggtatcca gagcaccttc agcggcaatc tgaccatagg caggtgagca aagacgtgct
     2641 tgagcataac ggagcacata tttcatcacc tccttatttt ttgatactat aaagccgatt
     2701 cttacgccac agagactgta tctcttagaa accgaatcca ggagtatgac attctcctcc
     2761 agcccgggga tatgcataca ggagaagtga ggttcgtcag tataacagaa ctcacggtat
     2821 acctcgtcgg aatagatata gagcccatat tttttggcaa ttgcgccgag ctccatcaga
     2881 gcctctttcg tgtatagaga acctgtcgga ttgcccgggt tgcatatgat tatacctttg
     2941 gtcttcggag ttattttctt ctcaacttcc gacatcgggg ggagagcaaa accatcctct
     3001 atcttggtgg tgatgggctt gaggataaca tcgttctgca aagcgaaaga gttatagtta
     3061 gtgtagaagg gctcaaacac gataacctca tcatcggggt tacaggtaac tgcaagtgca
     3121 atctggagtg cctcgctacc accgtttgtt atgtttatgc aagatgtatc aatatcgata
     3181 ttgatcccct tgtagtactt tacaagtcct tcgagaaggc tcttgttgcc tgcggaattg
     3241 ggatactcga caagtttgag ctgattgttc tttacagcat caagtgcctc ttttggggaa
     3301 gcaatgtcgg gctgaccgat gttgaggtga tacactttaa cgcctctttt ctttgctgca
     3361 tcagcaaaag gaacaagctt tctaataggg gaagccggca tcttttgggc tttttcggat
     3421 aatgttagca ttttaaaaaa atcaattatt attgtttatt gttgttaaat aatcagttct
     3481 caatctatct gtgattgcca ttgccatatt ttgcaagttc tctcataaag ccttcaattt
     3541 taggctgaac tgcgctggtg ggacaatcaa aattattcac caatattgca aacttaatgt
     3601 aacccttttt cgtctttaca tagcctgcat aacatctgac gctcgacaaa gagccgcttt
     3661 ttgccctgat aatctccttg atttcctttt tctcttcctt taataccccc tcgagtgtgc
     3721 cgggacgccc cggaccgggg aaactctcaa agtattgggc aaatgtagca ctttgttctt
     3781 tataagtata aaaatttgta aagaatcgag gcgaaacata gttttctctg gagaggccgc
     3841 taccgtcatc ctggcaaagc ccataggtgt ctacaccgtg atccctgaga tactgctcca
     3901 cagcctccgt ggatgcgtaa aaataggaat tatccacgag caccttgccg agcatcttta
     3961 gcatggtctc agcgtaaaag ttgttgctgg tggtattggt tacattgatg atctctatca
     4021 gttcaggaga gaaggtttcc gccaaataag tgagt
//
LOCUS       DAOSIE010000117         4026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_19701_length_4026_cov_3.263913, whole genome shotgun sequence.
ACCESSION   DAOSIE010000117 DAOSIE010000000
VERSION     DAOSIE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19701_length_4026_cov_3.263913"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PLI69_04420"
     CDS             complement(<1..823)
                     /locus_tag="PLI69_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOG41766.1"
                     /translation="MNINVQSEIGALEAVLLHRPGAEVENMTPRMAQRALYSDILNLS
                     IALREYEQLEGVLSKVAKIYQVKDLLLKVLEDKERKEALVARICVTEQVTEYFDQLME
                     MPPKELAKVLIEGRPARKNTLTSFLSDEFYALYPLYNFYFTRDSSVTIGNQVLICRMA
                     NKVRMRESLIMEAIYRGSGAFECDIINAHDFQPTNPDIFMEGGDILIVRDDILVIGNG
                     MRSSTRGIDFLISRLCQSGSKGRKHVIVQQLPSEPESFIHLDMVFTLLDRDKCMVF"
     gene            complement(837..1934)
                     /locus_tag="PLI69_04425"
     CDS             complement(837..1934)
                     /locus_tag="PLI69_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOG41767.1"
                     /translation="MNKNYYCIIMAGGAGSRFWPMSRNARPKQFLDILGLGRTFIQMT
                     YDRFVKIVPVENILVVTSEQYADLVKEQLPDILPENILLEPYKRNTAPCIAYATYKLY
                     DKNPDATVVVAPSDHMITGEENFFNTITCALDHASGSKDLFTIGIKPTRPDTNYGYIQ
                     INRQTNRIIGGHSSYAVKTFTEKPNEELAKVFIETGEFFWNSGIFIWNLRAIKEEMEN
                     HLPEVATLFAKGEGIYNTPREKEFIMKVYDNSPSISIDYGVMEKTSRAWIFLSNFGWS
                     DVGTWNSLYDRSVNKTPEGNFIKGSNILLRRVTNSIIWEKDPEKLLVVRGLDDFMVID
                     EKDVLLICPNDEKVLKDILAELAIEEQSKYL"
     gene            complement(1947..2318)
                     /locus_tag="PLI69_04430"
     CDS             complement(1947..2318)
                     /locus_tag="PLI69_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOG41768.1"
                     /translation="MNGRMTQGRVAENIALEWLQQEEGMELRERNWRSEHKEIDLIME
                     SDDFLHIVEVKSLKSPSQIAPFQQVDWHKQRFLVRAARRYVRSRNITKEVRFDIVSIM
                     FNKDGTTDLEFIPAAFFPIYY"
     gene            complement(2440..>4026)
                     /locus_tag="PLI69_04435"
     CDS             complement(2440..>4026)
                     /locus_tag="PLI69_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOG41769.1"
                     /translation="YQAEGICTSIANTTYGNWYLQDNTGEIYIYGTKDKTGNYNWKSF
                     GIEVGDKVLVEGPKVVYNNIIELVDVKVLKVTKSLIKVESDPIEVGKAGEPFEVLLTC
                     KGDGLSISIPDNAKSWLTVEAIILNGTNAVVKFNAAPNEGGARKTTLVFTTTSKGQVN
                     TAETTVTQEGSIIEMSIKDFLAQPESPTALYRLTGKVTNLAKSDYGNFDLVDATGKVY
                     VYGLTATKVEKNDKSFPTLGIKEGDVVTLIGTRSGYKGTPQVGGPAYHVSHTSHTEVA
                     SIADFLSKAVASKALESPYYKITGTITEILSDTYGNFYLKDATGTVYVYGLTVSGVAK
                     NDKSFASLGLKVGDVVTMIGQRGQYANAKVPEQKEQVSNGYYISHTAGSAGGGDTSSG
                     VLTLTVDGLPAAYSGDVTEVTLSGQKFKIVNVANYGSGIQISGAKSRDAGYITNVVGT
                     KKIKTIKITCATGKTWYPSNLVLYAGAAANPGETVIKPTSDETTSTYDLSGGDYKFFT
                     LKNPSDYTVYLDKIEITYAQ"
BASE COUNT         1054 a          951 c          877 g         1144 t
ORIGIN      
        1 cgaataccat acacttatcc ctgtcaagaa gggtaaatac catatccaga tggatgaaag
       61 actcgggttc ggagggcagt tgctgcacta tgacgtgctt acgacccttt gagccggatt
      121 ggcaaaggcg cgaaatcagg aaatctatgc cacgcgtgct gctgcgcata ccattgccta
      181 ttacaagtat atcgtcacgg acaataagta tgtcacctcc ctccatgaaa atatcgggat
      241 ttgtgggttg gaagtcgtga gcgttgatta tatcgcactc aaaagcgccg ctcccccgat
      301 agatagcctc catgatgaga gactcgcgca tacgcacttt attggccatc ctgcagatta
      361 gtacctgatt gccgatggta actgaggagt cgcgggtgaa atagaagttg tagagaggat
      421 agagtgcgta aaattcatca cttaagaatg aagtaagagt attctttcgg gcgggccgtc
      481 cctctataag cacttttgcc agctcctttg gaggcatttc catcagttga tcaaagtact
      541 ccgtcacctg ctctgtgaca cagattctgg ccaccagcgc ctctttgcgc tccttatcct
      601 caagcacctt gagcagcaga tcctttacct gatagatctt cgccaccttt gagagtaccc
      661 cctccaactg ctcatactca cgtaaagcaa tggagaggtt gagaatgtca ctgtagaggg
      721 cacgctgagc catcctgggg gtcatatttt ccacttcagc tccgggcctg tgcaatagca
      781 ccgcttcgag agctcctatc tcggattgta cgttgatatt catcagttaa attagattag
      841 aggtattttg attgctcttc gatggcgagt tccgccaaaa tatctttgag taccttttcg
      901 tcatttggac aaatcagaag cacatctttt tcatctatca ccataaaatc atccaggccg
      961 cgaacaacca gaagtttctc cgggtccttc tcccatatta tggaatttgt aactctacgc
     1021 agaagtatat tagagccttt gatgaaattt ccctcgggag tcttgttgac cgagcgatcg
     1081 tagagggaat tccatgtacc cacatccgac cagccgaagt tggagaggaa tatccaggcc
     1141 cttgaggtct tttccattac accataatct atagaaatgg aggggctgtt gtcgtaaact
     1201 ttcattatga actccttttc gcggggagta ttgtatatcc cttcgccttt ggcaaacaga
     1261 gttgcaacct caggcagatg attctccatt tcctccttga tggcgcgtaa attccagata
     1321 aatatacccg aattccagaa aaattctccg gtttcaatga aaaccttagc cagctcctcg
     1381 ttgggctttt cagtaaaagt ctttaccgca taggaagagt gtccgcctat aatgcgattg
     1441 gtttggcggt tgatctgaat atatccgtaa ttggtatcgg gacgagtggg cttgatgcct
     1501 attgtaaaga gatctttact gccggaagca tgatcaagag cgcatgtgat ggtattgaag
     1561 aaattttcct ctcccgtgat catgtggtct gaaggagcta ctaccactgt agcatcagga
     1621 tttttatcat aaagcttata ggtggcgtaa gcgatacaag gcgccgtatt acgcttataa
     1681 ggctcaagga ggatattctc cggaagaatg tccggaagtt gctctttgac cagatcggcg
     1741 tattgttctg aagtgacgac gagaatgttt tcaaccggaa caatctttac aaagcgatca
     1801 taagtcattt ggataaatgt cctaccaagt ccgagaatgt caagaaactg tttaggtctg
     1861 gcatttctgc tcataggcca gaagcggctt cccgcaccgc cggccataat aatacaataa
     1921 taatttttat tcatggcaag aaaatactaa taatatatcg gaaaaaatgc agccggaata
     1981 aactccaaat cggtcgtgcc atctttgtta aacattatgg atacgatgtc aaagcgcacc
     2041 tcttttgtaa tgtttctcga cctgacatac cttctcgccg ccctaacaag aaaacgctgt
     2101 ttgtgccagt ctacctgctg gaacggggct atctgagagg gagatttcaa cgatttgacc
     2161 tccactatgt ggagaaaatc atcactctcc attatcaggt ctatctcctt atgttctgac
     2221 ctccaattgc gctccctgag ctccataccc tcttcttgtt gcagccattc aagcgcaata
     2281 ttttcagcca ctcttccctg cgtcatcctt ccgttcataa tatacaaatg taacaaaata
     2341 ttcggacaat acgacttagc cagagaaata aagtaaaggg tgaccgcaaa aagacgatca
     2401 ccctttccat cccctgaatc gaggaggttt caaacccttt tactgagcat aggtaatctc
     2461 tattttatcg agatataccg tatagtcgga ggggtttttc aaagtgaaga acttatagtc
     2521 tcctcctgaa agatcgtaag tagaagttgt ctcatccgaa gtaggtttga tcacagtctc
     2581 acccggattt gcagcagcac ctgcataaag cacaagattg gagggatacc atgttttgcc
     2641 tgtagcgcag gtgatcttta tggtcttgat cttctttgtg cccacaacat tggtgatgta
     2701 gccggcatct ctgcttttgg ctccgctaat ctggataccg ctaccgtagt ttgccacatt
     2761 cacgatcttg aatttctgac cggatagggt aacttcggta acatctcccg aatatgctgc
     2821 cggtaatccg tcaactgtca gggtgagaac accggaactc gtatctcccc ctcctgcact
     2881 acctgccgta tgcgagatgt agtagccatt tgacacctgt tccttttgct ccggaacttt
     2941 tgcattagca tattgaccgc gctggccgat catagttaca acatcaccca ctttaagtcc
     3001 gagcgaagcg aacgatttat cattcttcgc tacaccgctt acggtaagac cataaacata
     3061 tactgttccg gtcgcatcct tgaggtagaa gttgccataa gtatcactca aaatttcggt
     3121 aatggtgcct gtgatcttgt aataaggaga ctcaagtgct ttggaagcaa ccgccttgga
     3181 gaggaagtct gcaattgatg ccacctcagt atgagatgta tgacttacgt ggtaagccgg
     3241 acctccaacc tgtggagtgc ccttgtatcc ggatctggtt ccgataaggg taacaacatc
     3301 accttccttt attccaagtg tagggaatga tttgtcattc ttttcaactt ttgtggcagt
     3361 caaaccataa acatatacct tgccggttgc atctaccaga tcaaagttac cgtaatcgct
     3421 ttttgcaaga tttgtaacct ttcctgtcag acggtatagg gctgtcggtg attcaggctg
     3481 tgcgaggaaa tccttaatcg acatctcgat gatggagcct tcttgagtta ctgtggtctc
     3541 tgcagtattt acctgaccct tggaagtagt agtgaaaaca agagtagtct ttcttgcccc
     3601 gccttcattt ggagctgcat tgaatttaac aacggcatta gtaccattga gaataatagc
     3661 ctcaaccgta agccatgact tggcattatc ggggatggaa attgagagac catcaccttt
     3721 acaagtaagc agtacctcga agggctcccc ggctttacct acctcaatgg gatccgactc
     3781 cactttaatg agggatttgg taaccttaag gacttttacg tcaacaagtt cgataatatt
     3841 attgtaaaca actttagggc cctctacgag taccttgtca ccaacctcga tgccaaagct
     3901 cttccagttg tagttaccgg tcttgtcctt ggttccgtag atatagatct cgccggtatt
     3961 atcctgaagg taccagttac cataggtagt atttgcgatt gacgtacata taccctcggc
     4021 ctgata
//
LOCUS       DAOSIE010000118         4020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_19736_length_4020_cov_3.461286, whole genome shotgun sequence.
ACCESSION   DAOSIE010000118 DAOSIE010000000
VERSION     DAOSIE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19736_length_4020_cov_3.461286"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            771..2120
                     /locus_tag="PLI69_04440"
     CDS             771..2120
                     /locus_tag="PLI69_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOG41770.1"
                     /translation="MKNQKRIFLSEKDIPTQWYNIVADMPEKPLPILNPKTKEPVTVD
                     DLAVIFNRACSEQELNQTDAFIDIPEEVREMYKYYRPTPLVRAYGLEKALETPAHIYF
                     KNESVSPIGSHKLNTAIAQAYYCKQEGVTNVTTETGAGQWGAALSYAARAFGLELAVY
                     MVKVSYHQKPYRRSLMQTYGAQVIASPSMSTRAGKDIITAHPNHQGSLGTAVSEAIEL
                     ALMTPNCKYSLGSVLNHVALHQTVIGLEAEKQMEIAGEYPDIVIGCFGGGSNFSGIAF
                     PFLRHNIKKGLKTRFIAAEPASCPKLTKGVFKYDFADEAGYSPLLPMYTLGHNFSPAN
                     IHAGGLRYHGAGTIVSKMLMDGMVEGMDINQLETFEAATLFARVEGIVPAPESSHAIA
                     AAIREANKAKEEGASKTILFNLSGHGLMDMSAYDQYIAGDLVNYELSEEEIRKSVGE"
     gene            complement(2154..3440)
                     /locus_tag="PLI69_04445"
     CDS             complement(2154..3440)
                     /locus_tag="PLI69_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOG41771.1"
                     /translation="MVKLKENLRTEDWVTVWMSIPLLLMAFIIPQYIPSVPSTLTTLA
                     AWGHIGIIFAIVFVVLYAGQALMGRKLRGMLPSLIVIFALSLVAQIIAKIPIVKFYGL
                     ESVFFSVILGLLIRNLFRIPEWMKPAIQGEFFIKIGVVCLGATILFSEVMKSGVFGLI
                     QAVLVVSSVWFFAFWISRKFGVDEKSGLILSSGVSICGVSACITAARVAGGEDKKLSY
                     IVSVLLIVVVAMIYLMPWLSNMILPLLIDDTQLMQEVAGAWIGSTVDTTSGVAASSAI
                     HSEVANQNAIIIKAAQNVLIGVVAFFIAVYLSARSEKGTSQRPSISIVWEKFPKFIIG
                     FVVASLVFSIFQANDLFTMDSKGKLLEPGVAKNFSNIFFSLAFVCIGLGTRLKEIISR
                     ENRRILWAFLCAQGFNIIVTFVIAYVIFGVVKPMFA"
     gene            complement(3460..>4020)
                     /locus_tag="PLI69_04450"
     CDS             complement(3460..>4020)
                     /locus_tag="PLI69_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41772.1"
                     /translation="RDIGFKVYGSLFHQENHSIVSYDIGLFNGNGTNRRDNDGRKDLI
                     ARLKISPIRYLTIAGYYQNGTVKADHIEGNLLTRYGGGISYDNNKVMCRSEYISGYTG
                     KMLSEGIYLTSGYWVTDKVMALGRADYFKREKALKDKEMYYTLGLIYTPWKNLRLQLN
                     YTLKEFTTSVDKSYGSLFNFMATALF"
BASE COUNT         1170 a          894 c          880 g         1076 t
ORIGIN      
        1 agccgccaac tttaagaaag ccccgacagc atcagttgtc ggggcttttg tatttgatta
       61 tggtttagag agaatacaaa aatttaaaga atattttaac tgctctcttt atttgatttt
      121 ttgaggtttg atttttggtg gttctcatac atttctattt gaagcttcag ataacctaat
      181 agacctatgg ccaaaatcag agaaccgtaa gaaaactctt tgaagatatt ttccttcata
      241 ataaatccga atattcctgc aaaaaagaat ataactatag tgaagatagt gactattatc
      301 aaagcttttg ctctttttgg attttgccat attcgctttt cgtaacgcaa tgatgtctta
      361 ctggagtctt gcagggcagc aaagctgagc gttaaaccga aaaatatcag tacgttgcta
      421 taagttttaa acaggaattc cggattggaa aacaggtaat caaacccccg gaaaaagggc
      481 ttaaacaata aaaataatcc gattagcaaa aatggatatt gcaggtagct gagtgcgtga
      541 aatacttgtt tgaaattttt catttgcgct ttatagtgtc aaatctgatt tggcacaaat
      601 ttaataacat tctatgacat gacgatccgg cgtagcggaa gatgcaatta ttatatcaaa
      661 ttttgcatcg tataacagta acgccggttt tattcaaaaa aagatcattt ctctttgagt
      721 ttttagggct gttggttatc tttgtcggtc aaacacacaa attaatagtt atgaagaatc
      781 agaagagaat ctttctgagt gagaaagata ttcccacaca atggtacaat attgtggcag
      841 atatgccaga gaagcccctg ccgatactta accctaaaac caaggagccc gtaaccgtag
      901 atgaccttgc agtcattttc aacagggctt gttccgagca ggaactcaac caaaccgatg
      961 cattcataga cattcccgaa gaggtgaggg agatgtataa atattacagg cccactcctc
     1021 tcgtaagggc ctacggattg gaaaaggcac tcgaaactcc tgcacacatc tattttaaaa
     1081 atgagagcgt aagtcctatt ggttcgcaca aactcaatac ggccatagct caggcatact
     1141 attgcaaaca agagggtgta accaatgtta ccaccgagac cggagccggt caatggggag
     1201 cagctctttc atacgctgca agagcatttg gtctcgaact ggctgtctat atggttaaag
     1261 tcagttatca tcagaagccc taccgtcgct cacttatgca gacttatggt gcacaggtca
     1321 ttgcatctcc cagtatgagt acccgtgccg gaaaggatat aattacggca caccccaatc
     1381 accagggaag tctcggtacg gcagtatcgg aggccatcga actggccttg atgactccaa
     1441 attgcaagta ttcgctcggt agcgtactca accatgttgc actccatcag actgttatcg
     1501 gccttgaggc tgagaagcag atggagattg ccggagaata ccccgatatt gtgataggct
     1561 gcttcggtgg aggatccaat ttcagcggca tagctttccc tttcttgcgt cacaatatca
     1621 aaaaggggct caagacccgt ttcattgccg cagaaccggc ttcctgtccc aaacttacaa
     1681 aaggagtatt taagtatgat ttcgctgatg aggcgggata ctctcctttg ctccccatgt
     1741 acacgcttgg ccacaacttc tcgccggcca acatccatgc aggcggtttg cgttaccacg
     1801 gtgcaggtac tatcgttagc aagatgctga tggacgggat ggtggagggg atggatatca
     1861 atcagcttga gacatttgag gctgccaccc ttttcgccag agtagaagga atagttccgg
     1921 ctcctgagtc ttcacacgct attgctgccg ctatcaggga ggcaaataag gcaaaagagg
     1981 agggggcctc caagaccatt ctcttcaacc tttccggtca tggccttatg gatatgtctg
     2041 cctacgacca atatatcgca ggagaccttg tcaattacga gctctcggag gaagagatcc
     2101 gtaagtctgt cggagaataa agttctatcc ctggtcaggc aacccgggct ttgctaggca
     2161 aacataggct ttactacccc gaagatcaca taagcaatca caaaggttac aatgatattg
     2221 aaaccttgag cgcagaggaa tgcccacagg atcctacgat tttcccggga aatgatctct
     2281 ttcagacgtg tgcctagtcc tatgcagaca aaggcaagtg agaaaaagat atttgagaaa
     2341 tttttagcaa cgcccggttc gaggagtttt cctttactgt ccattgtaaa gaggtcattg
     2401 gcctgaaaga tgctgaatac tagcgaagct acaacaaaac ctatgataaa tttcgggaat
     2461 ttttcccaga caatacttat ggaggggcgc tgagaagtgc ccttttcgct tcttgcggag
     2521 agataaacag ctataaagaa ggcaacgaca cctatcagta cattttgcgc agctttaatg
     2581 ataatagcgt tctgattggc aacctcactg tgaatagcac tggaggccgc aactcccgaa
     2641 gtagtatcca cagtacttcc tatccaggct ccagccacct cctgcatcag ctgagtatca
     2701 tcgatcagaa gcggcagtat catattggag agccagggca tcaggtagat catagcaacc
     2761 actacgatca gtagcacgga aactatatag gagagcttct tatcttctcc accggcaacc
     2821 ctcgcagcgg tgatacaggc ggaaactccg caaattgaga caccgctgga aagaatcaat
     2881 ccgcttttct cgtcaacccc gaacttgcgg gagatccaga aagcgaaaaa ccagacactt
     2941 gataccacaa gtaccgcctg tataaggcca aagacacccg acttcatcac ttcactgaaa
     3001 agtatggtgg ctccaaggca cacgacacct atttttatga agaactcacc ttgtatggcg
     3061 ggcttcatcc attcaggtat cctgaacaga tttcggatca aaaggcccaa tattactgag
     3121 aagaaaaccg actcaagtcc gtaaaacttt actatgggta tcttggcgat aatttgagct
     3181 acaagtgaaa gggcgaagat tacaataagt gaaggtagca tccccctcaa cttgcgtccc
     3241 ataagtgcct ggcctgcata gagtactaca aaaacaatag cgaagatgat gcctatgtgg
     3301 ccccatgcag caagggtggt gagagtggat gggacgcttg ggatatattg gggtatgata
     3361 aatgccatca gaagcagagg aatactcatc caaacggtta cccaatcctc tgttctgagg
     3421 ttttctttca atttgaccat gtatgaaagt gttcggatgt tagaacaacg cagttgccat
     3481 aaaattgaag aggctgccgt atgatttgtc aaccgaagtt gtaaactctt tcagagtgta
     3541 gttgagctgg agacgaaggt ttttccaggg cgtataaatc aatcccagcg tataatacat
     3601 ttccttgtct ttcagtgctt tttctctttt gaaataatct gccctgccca atgccatcac
     3661 tttatcagta acccaatatc ctgaagtcag gtagattcct tccgatagca ttttgcctgt
     3721 gtagccactt atatactcac ttcgacacat cactttattg ttgtcataag agatgccgcc
     3781 accatatcgg gtaagcaagt ttccttcaat gtgatccgct ttaacggtac cgttctggta
     3841 gtatccggca atcgtgagat atcttatggg tgagatttta aggcgggcaa taagatcttt
     3901 acgtccgtca ttgtctcttc tgtttgtacc gttaccatta aagagtccta tatcataact
     3961 cactatacta tgattttcct ggtgaaacag gctcccgtat accttaaaac ctatatcacg
//
LOCUS       DAOSIE010000119         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_19774_length_4014_cov_3.471836, whole genome shotgun sequence.
ACCESSION   DAOSIE010000119 DAOSIE010000000
VERSION     DAOSIE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19774_length_4014_cov_3.471836"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1424
                     /locus_tag="PLI69_04455"
     CDS             84..1424
                     /locus_tag="PLI69_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41773.1"
                     /translation="MKLRHYITGVALIAIVFHSCSGAPSESIPSDSYPDIIPDYIGVT
                     IPEGIAPLTFEMADGTNCRFETEREGDTLWYSVTSWRKGDKKGTIHKPFPVYISADLI
                     DPYIAYRLIEPGYESWHNIVIAQRELASYKESRIVSNRANDMGCVNCHTFDSGDPQKM
                     LFHARGKGGGTVFIDGDQIRLVKLAEVGSQRQGTYPAWHPSGRYVAFSSNNTRQCFTI
                     NEYQPIEVYDTASDIILMDLQTDSVILVPQLSLETRMETFPSWSEDGSTLYFCSAASV
                     ENVANNRGQVHYRLMSAKFENGQFIGEPEELFGDDSCSVSFPRYNDGYVLFTHTAFGT
                     FPIWHDEADLWMYDTRSKEAFPAEILNSDKAESYHSWSSNGKWVIFGSRRLDGRYTRL
                     YIAHYKGNGEFDKPFLLPQKNFKHNTLRLKSYNIPEFIKGEVKDYEKAVSKLFK"
     gene            1421..2830
                     /locus_tag="PLI69_04460"
     CDS             1421..2830
                     /locus_tag="PLI69_04460"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOG41774.1"
                     /translation="MMKNREKYILPALLFLVVFAIEALFQRYTAIIQGHNSLFLLTGD
                     YFRETFAAPWPLSHLLESFFLQFYEIPMVGPMINALATTLIFLLFNFILQRIRIPFHN
                     LIAVAGACVCWIFAAMATTPLLLYKVLLISSGVAPAALLLKKREIVRAGVWEIAVVGL
                     FIITAALFISLSKETRSVETTAKVRYATWRGDWNTVLENVTPQSSIDRPELMPYAFLA
                     MGFTGELGQRLFSYPIQKIEDFDMEGIQSIEGNYFNSLLNYYMGNYNEALHHIFQYSC
                     HFPHGMTNFSLSQMIRYNNALGNYTMVRKYAQILSHSPRYSPSARKLLKRFEGVQDSI
                     PPAGHLSSGARTTTNNPQYDLLQLSLAGINSPIAQERFLCYLMLQGDLQYFVEAFSIF
                     HWERIPRHYEEALLLAGVNPNKFGISITSQRLFNEISSAMAQMDMPAVDRMAKGTYWE
                     YHFRKQKEGAFESGLEFDL"
     gene            3174..>4014
                     /locus_tag="PLI69_04465"
     CDS             3174..>4014
                     /locus_tag="PLI69_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOG41775.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGKEPAVIINSEGKRTTPSIVAFAD
                     NGERKIGDPAKRQAITNPERTIFSIKRFMGETYDRVAKETQRVSYKVVKGDNNTPRVD
                     IDGRLYSPQEISAMVLQKMKKTAEDYLGQTVTEAVITVPAYFSDSQRQATKEAGEIAG
                     FKVRRIINEPTAAALAYGLDKQHKDMKVAVFDLGGGTFDVKSTNGDTHLGGDDFDYKI
                     INWLAEEFMADNSGIDLRKDPMALQRLKEAAEKAKIELSSQTSTEINLPYIMPVDGIP
                     KHLV"
BASE COUNT         1124 a          996 c          910 g          984 t
ORIGIN      
        1 ttcaccctgt aaccagggag cctctcacaa ttgaggcacc tgtggattgg acaccaatat
       61 tgaagagata aattttacat aaaatgaaac taagacatta tataacggga gttgccctga
      121 ttgcgatagt gttccactcc tgctcgggag caccctccga aagcatcccg agcgactctt
      181 atcccgatat catacccgat tatatcggcg ttaccatacc cgaagggatc gcaccgctca
      241 cctttgaaat ggctgacggc accaattgcc gcttcgaaac ggagagagag ggtgacactc
      301 tatggtactc cgttacatcg tggcgcaagg gtgataaaaa aggcactatt cacaaacctt
      361 tccccgtata tatctcagcc gaccttatag acccctacat cgcctacagg ctgatagagc
      421 cgggctacga aagctggcac aacatcgtca tagcgcagag agagctcgca tcctataagg
      481 agagccgtat cgtgtcgaac cgagcaaatg atatggggtg tgtcaactgc cacacttttg
      541 actccggaga cccgcaaaag atgttgtttc acgccagagg gaaagggggt ggcactgtct
      601 tcattgacgg agatcagata cggctggtca agcttgcaga ggttggatca caaagacaag
      661 gcacctatcc cgcctggcac ccctccggcc gttatgtagc cttctcctcc aataacaccc
      721 gtcagtgctt taccatcaac gaatatcagc cgatagaggt ttacgatacc gcttcggaca
      781 tcatactgat ggaccttcag accgactcgg tcattcttgt accgcagctc tctctggaga
      841 cgcggatgga gaccttcccc tcatggtctg aagatggatc cacgctctat ttctgctccg
      901 ctgcatcagt cgaaaatgtg gccaacaacc gtggccaggt gcattacaga ttgatgtctg
      961 caaagtttga gaatggtcaa tttattggag aaccggaaga actattcggc gacgacagct
     1021 gttcggtctc attcccccgt tataatgacg gttatgtgct ctttacccat actgcattcg
     1081 gcaccttccc catctggcat gatgaggccg acctctggat gtacgacacc cgatccaaag
     1141 aggcatttcc ggcagaaatt ctaaatagcg acaaagccga gagctatcac agctggtctt
     1201 ccaacggcaa atgggtcata ttcggcagtc gccgtctgga tggacgctac acccggctct
     1261 atattgccca ttacaaaggt aacggagagt tcgacaagcc cttcctgctt ccgcagaaaa
     1321 atttcaaaca caatacgctg cgtctgaaat cctacaatat cccggagttc ataaagggtg
     1381 aggtaaaaga ttacgaaaaa gctgtttcaa aactcttcaa atgatgaaaa atagagaaaa
     1441 atatatattg cctgcgctac tgtttttggt agtatttgcc attgaagccc ttttccagcg
     1501 atatacggcg ataattcagg gacacaacag cctattcctg ctgacaggag attatttcag
     1561 ggagaccttt gcagcaccct ggcccctttc tcatcttctg gagtcattct tcttgcagtt
     1621 ttatgagata ccaatggtag gtccgatgat aaatgctctg gccacaactc tgatatttct
     1681 gctcttcaac tttattttac aacgtataag aatccctttc cacaacctga tagccgttgc
     1741 cggagcctgc gtctgttgga tatttgcagc aatggctact actcctctac tactctataa
     1801 ggtgcttctt atctcatcgg gagtggcccc ggcagcactt ctgctaaaga aaagagagat
     1861 tgtacgagcc ggagtatggg agattgcggt tgtcggcctt ttcatcataa cggcagccct
     1921 ctttatctcc ctaagcaaag agacacgaag cgtcgagact accgctaaag tgcgctacgc
     1981 cacctggagg ggagattgga acacagtact ggaaaatgtt actcctcaaa gttccataga
     2041 ccggccggag ctgatgccct acgccttcct tgccatgggt tttacaggtg aactggggca
     2101 acgcctcttc tcctatccca tacaaaagat cgaagatttt gatatggagg gaatacaaag
     2161 catagagggc aactatttca actccctgct aaattactac atgggcaatt acaatgaggc
     2221 tctccaccat atttttcagt actcctgcca ttttccgcac gggatgacca atttctccct
     2281 ctcgcagatg atacggtaca ataatgcgct ggggaactac acgatggtgc gtaaatatgc
     2341 tcagatattg agccacagcc ccagatactc cccatcggcc cgcaaactgc tgaagagatt
     2401 tgagggggtt caagactcca ttccacccgc tggtcatctt agcagcggag cgcgaaccac
     2461 caccaacaat cctcagtacg atctcttgca gctctcgctt gcaggcatca actcccctat
     2521 tgcacaagaa cggtttcttt gctatctgat gctgcaaggg gatttgcaat actttgtgga
     2581 ggcattcagc atttttcact gggagcgcat ccctcgccac tatgaggagg cactgctcct
     2641 tgccggtgtc aatcccaata aattcggtat atcaattacc tcgcagcgac ttttcaatga
     2701 gatctcttca gctatggcgc aaatggatat gcccgcagta gaccgtatgg caaaaggcac
     2761 ctattgggaa tatcacttcc gcaaacaaaa agagggagct ttcgagtcgg gattggagtt
     2821 cgatctataa aatctccgat gagagtatac attgtcgcac ggaggtgtgg caaattagag
     2881 gcagttcatt tcatttattc tgaacaaccc tcttaacagg ctcatatatc acattttaac
     2941 cctaattacc cataagaggg ccggcttgtt gatgctatta tatctctccc ggatatatag
     3001 taaaaaatga cattcatttc atactgtttc aggcaaaaaa tagggcaggt ctgacacatt
     3061 gtcagtccag aaccccattt atggtattgg cacaatcttg gcgctattga atgtcgtaac
     3121 gaaaagtata taatagtaat tgtaaaaata tttaaagaat aagaaaagga attatgggaa
     3181 aaattatcgg tattgacctt ggcaccacga actcgtgcgt agccgttatg gaaggtaaag
     3241 aacccgctgt tatcatcaac agtgaaggca aaagaaccac tccgtcgatt gttgcatttg
     3301 ccgacaacgg cgaaagaaaa ataggtgacc ccgccaagag acaggcgatc accaatccgg
     3361 aaaggaccat tttctctata aagagattta tgggcgaaac ttatgacaga gtagcaaaag
     3421 agactcagag agtatcatac aaagttgtaa aaggcgacaa caatactcct cgcgtagata
     3481 ttgacggacg cctctattct ccacaggaga tctcggcaat ggtactccag aagatgaaaa
     3541 agacagctga ggactatctg ggacagacgg ttaccgaagc agtcatcact gtaccagctt
     3601 actttagcga ctctcagcgt caggctacta aagaggccgg agagattgca gggtttaagg
     3661 taagaagaat tatcaacgag cctactgctg ccgctctcgc ttatggtctt gacaaacagc
     3721 acaaggatat gaaggtagca gtattcgacc tcggtggcgg tactttcgat gtgaaatcta
     3781 ccaacggtga cacacacctc ggaggtgacg acttcgacta taagatcatc aattggcttg
     3841 ccgaggagtt tatggcagat aactcgggta tcgacctaag gaaagatccc atggctctcc
     3901 agagactgaa agaggcagcc gagaaggcta aaatcgagct ctccagccag acctcaaccg
     3961 agatcaatct cccttacatc atgcctgttg acggtattcc caagcacctt gtga
//
LOCUS       DAOSIE010000120         3996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_19884_length_3996_cov_3.492515, whole genome shotgun sequence.
ACCESSION   DAOSIE010000120 DAOSIE010000000
VERSION     DAOSIE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19884_length_3996_cov_3.492515"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLI69_04470"
     CDS             complement(<1..435)
                     /locus_tag="PLI69_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HOG41776.1"
                     /translation="MKKVKVGINGFGRIGRLVFRAAQKRNDIEIVGINDLLDVDYIAY
                     MLKYDTVHGKFDGTIEVADGKLIVNGHAIRVTSEKDPTNLKWNEVGAEYVVESTGLFL
                     TKETAQSHIKAGAKRVIMSAPSKDDTPMFVYGVNHTKYNGENI"
     gene            543..1334
                     /gene="surE"
                     /locus_tag="PLI69_04475"
     CDS             543..1334
                     /gene="surE"
                     /locus_tag="PLI69_04475"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOG41777.1"
                     /translation="MKHIEILVTNDDGLNARGVKEAATLMRTYGNVTVVAPSEPQSGK
                     SASISLENTLRLNLIESVPAEEGLGSLRVYSFTGTPVDCVKMGVNLFINEDRMPDLLV
                     SGINHGSNASVASIYSGTLGAAAEGAVYDIPSIGLSMNTHKAEPDFEGVLHYSRIIMD
                     KLLKTGISKGIYLNVNFPNIPLQEIKGLKIGHQGHGRWVREFEKRTDPRGREYYWIIG
                     RFLNLDKAPDADHNLVDDGYVSIVPHRIDNTDYQEIERLRDLWPA"
     gene            1352..2506
                     /gene="lpxB"
                     /locus_tag="PLI69_04480"
     CDS             1352..2506
                     /gene="lpxB"
                     /locus_tag="PLI69_04480"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOG41778.1"
                     /translation="MRYYLIAGEASGDLHGSNLMKGLREEDPEAEFRFRGGDLMAAVG
                     GTLVHHYKSTAVMGIVEVISRMGTIRRNLAECRRDLLEYDPDLLILIDYPGFNLKMAR
                     FAHRHGIRVFYYIAPKLWARGERRIRKIRRYVDELFVIFPFEKEYFRNLGVEAHYEGN
                     PLIESIDAYKALHMGREEFTTRHSLADSPSIALLAGSRKVEISFLLPRMVAMERLLKQ
                     DPLLSGYQLLLAAAPSVDLQLYRSLIPADSSIRIVSDDTYGVLSHSEAAIISSGTASL
                     EAALIGIPQVVCYGFNRITYLIARILVKVKYISLANLILDKLIFKELVQDDASPEKIF
                     EEVLRLITDKEYKSDMKNDYAHLRDVLGGSGASRKIASNMITRINNKLNK"
     gene            2518..3348
                     /gene="dapF"
                     /locus_tag="PLI69_04485"
     CDS             2518..3348
                     /gene="dapF"
                     /locus_tag="PLI69_04485"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOG41779.1"
                     /translation="MAKRVKVYKYQGAGNDFVIIDNRDGSIDLNDSQIRWLCDRRFGI
                     GADGMMLLGAGGEYDFTMRYYNSDGFEGTMCGNGGRCLVAFAAHRGIKKFEFIAIDGY
                     HRAEVLEYGDYRCIVRLKMRDLLPDEEGNPPIKIYPEGYFLDTGSDHFVIFVDDVANY
                     DVDGEGKKWRWAERFPKGTNVNFVEIAPNKIKVRTYERGVEAETLACGTGVTASSIAS
                     FIHGAKSFTTDGERVKFDIEALGDSLAVDYIPSDDGKRFEEIWLTGPATFVFETEVEL
                     "
     gene            3387..>3996
                     /locus_tag="PLI69_04490"
     CDS             3387..>3996
                     /locus_tag="PLI69_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOG41780.1"
                     /translation="MEIFRYIGIKRKVFLGSIVLAMVLLISSSISMFEYTRMNDHVAE
                     LISDNTNSIEVSRELLAKAEEYNLRLMHLVLGEVGEESIEVINDDAFISFFSGIKESF
                     VTEQDKANVDSVMYAFAAYMHIVREASDIWLTNRDDLLKEWYFNRLQPVYAKLKAYIE
                     KLTNDSQDALIHNSQSLQDNFYRSLMPGLISALLGFLMVLLFN"
BASE COUNT         1073 a          823 c          936 g         1164 t
ORIGIN      
        1 aatattctca ccgttgtatt tggtatggtt tacaccatag acaaacatgg gagtgtcatc
       61 tttggaggga gcagacatta taactctttt tgctcctgct ttaatatgtg attgtgctgt
      121 ctcttttgtt aggaaaagac ctgtagactc aaccacatat tcggcgccca cctcgttcca
      181 ctttagattt gtgggatcct tctcggatgt tactcttata gcatggccgt tgacaataag
      241 tttgccatca gctacttcta tcgtaccgtc aaatttgccg tgaactgtgt catacttcaa
      301 catgtaagct atgtagtcca catcaagcag gtcattgatg cctactatct ctatgtcatt
      361 tctcttctgg gctgctctga aaaccagacg tccgatacgg ccgaaaccgt taataccaac
      421 ttttactttt ttcattttat ctattatatt agtatattat atttgaaata ttttataact
      481 taaaaaccct gcaaatttag caacaattat ctaaataaca atgaaaatat tacctttgcg
      541 aaatgaaaca tattgagatt ttggtcacca atgatgatgg cctcaatgcg agaggcgtca
      601 aagaggcggc cacgcttatg cgcacatacg gcaatgtgac tgttgttgcc ccatctgaac
      661 ctcagtccgg taagtccgca tccatttctc tggagaatac actcagattg aacctgattg
      721 aaagtgtacc ggccgaagag ggactgggtt cgctacgtgt atatagtttt accggcacac
      781 cggtagattg tgtaaaaatg ggtgtcaacc ttttcataaa tgaggacagg atgccggacc
      841 ttttggtttc aggaatcaat catggctcaa atgcctcagt ggcctcgatc tactccggta
      901 ctttgggtgc agctgccgag ggtgctgttt acgatatccc ctctattggg ctttctatga
      961 atacccataa agcagaacct gattttgagg gagtactcca ttactcacgc attataatgg
     1021 acaaactatt gaaaacaggt atcagcaagg gcatatatct gaatgtaaac ttccctaaca
     1081 tccctcttca agagatcaaa ggtctcaaaa taggccacca agggcatggc agatgggtta
     1141 gggagtttga aaaacggacc gatcctcgcg gaagggaata ctactggata atagggaggt
     1201 ttctcaatct ggacaaggct cccgacgctg atcacaatct ggtcgatgat ggttatgtct
     1261 ccattgttcc gcaccgcata gataataccg actatcagga gattgaacgt ctgcgtgacc
     1321 tttggcctgc ctgatcccaa attctgctca catgcgctac tatctcattg ccggagaggc
     1381 ctcgggagat ttacatggct ccaatctgat gaaaggattg agggaggagg atcctgaggc
     1441 tgagttcaga ttccgcggtg gagatttgat ggcggctgta ggagggactc tggttcacca
     1501 ttataaatct actgctgtaa tggggattgt agaggttatc tcccgtatgg gaactattcg
     1561 acgcaatttg gcagaatgcc ggcgtgatct gctggaatat gatcccgatc tacttatcct
     1621 catagattat cccggcttca accttaagat ggcccgcttt gcccacagac atggcattag
     1681 agtcttttat tatatcgctc caaagctctg ggccagggga gaaagacgca tccgaaagat
     1741 tcgcaggtat gtagatgagc tatttgtcat atttcctttc gagaaggagt actttcgcaa
     1801 tctcggagtt gaggcgcact atgagggtaa tcctctgata gagagcatcg atgcctataa
     1861 ggctctccac atgggaaggg aggagtttac gacacggcac tctcttgccg atagcccttc
     1921 catagcattg cttgcgggta gcagaaaggt agagataagt tttttgctcc ctcggatggt
     1981 agccatggag aggcttctga aacaggatcc gctcctttcg ggttatcagt tgctgttggc
     2041 ggctgcccct tcggttgatt tgcaactcta ccgttcgctt atacccgctg atagttccat
     2101 aaggatagtc tcagatgata cctatggagt acttagccac tccgaagcgg ctattatcag
     2161 ttccggtacg gcaagtttgg aggcagctct tataggtatc cctcaagttg tatgctatgg
     2221 cttcaacagg attacctatc ttattgcgcg tattttggtt aaggtaaaat acatcagtct
     2281 tgcaaacctc atcctcgata aactcatttt taaggaactt gttcaagatg atgcctcacc
     2341 ggaaaagatt tttgaggagg ttttgcgtct gattactgat aaggagtaca aaagtgatat
     2401 gaagaacgat tacgcccatt tacgtgatgt gcttggaggt tccggcgcat cgcgaaaaat
     2461 agcctccaat atgattactc gaattaataa taagttgaac aaataatacc tttctttatg
     2521 gcaaaaagag tcaaagtcta caaatatcag ggagccggca acgattttgt aataattgac
     2581 aaccgcgatg gctcaatcga tcttaatgat tcacagataa gatggctctg tgaccgtagg
     2641 ttcggcattg gggctgacgg aatgatgctt ctcggagcag gtggagagta tgatttcacc
     2701 atgcgttact ataattcaga tggatttgag ggcacaatgt gcggcaatgg aggcagatgc
     2761 ctcgtggctt tcgcagctca cagaggcatt aaaaaatttg aattcatcgc aatagacgga
     2821 tatcaccgtg ccgaggtgct cgagtatgga gattatcgtt gcatagttcg tctcaagatg
     2881 agggatcttc ttcctgacga ggaaggcaat cctcccatta aaatctatcc tgagggttat
     2941 tttcttgata cagggtcaga tcacttcgtg attttcgtag atgatgtggc caactatgat
     3001 gtagatggtg agggcaagaa atggagatgg gctgagcgtt ttcccaaggg gacaaatgtc
     3061 aattttgtag aaatcgctcc aaacaagatc aaagtcagga cctatgaaag gggagtggaa
     3121 gctgaaacct tggcttgtgg taccggagtt acagcctctt caatagcatc tttcatacat
     3181 ggtgcaaaat cttttacaac cgatggagaa agggtaaaat ttgatataga ggctctaggg
     3241 gactctttag ccgttgatta tattcctagc gatgatggaa agagatttga agagatctgg
     3301 cttaccggcc ctgctacatt tgtattcgaa actgaagtag aactctagcg tttattttct
     3361 atctttgtaa gactaaccgt tgatttatgg aaattttccg atacataggt ataaagagga
     3421 aagtcttcct cggctccatc gtcctggcca tggttctgct gatttcgagt tccatctcta
     3481 tgtttgagta tacaaggatg aatgatcatg ttgctgaact catttccgat aacaccaaca
     3541 gtattgaggt atcaagagaa cttctcgcaa aagcggagga gtacaatctt cgcctgatgc
     3601 acctggtctt aggagaagtt ggtgaagaga gtatagaggt aatcaacgac gatgcattca
     3661 tctctttttt cagcggcatc aaggagagtt ttgtaaccga gcaggataag gctaatgttg
     3721 attctgtgat gtatgccttt gctgcatata tgcatatcgt gcgtgaggcc tccgatatat
     3781 ggcttaccaa cagagatgat cttctcaagg agtggtattt caaccgtttg cagcccgttt
     3841 atgctaaact taaagcatat attgaaaagc tgactaatga tagtcaggat gcattgattc
     3901 acaattctca gtcgctccag gacaattttt acagaagtct catgccgggc ctgatctcag
     3961 ctttactggg ttttcttatg gttctgctct ttaatt
//
LOCUS       DAOSIE010000121         3969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_20066_length_3969_cov_3.527338, whole genome shotgun sequence.
ACCESSION   DAOSIE010000121 DAOSIE010000000
VERSION     DAOSIE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20066_length_3969_cov_3.527338"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PLI69_04495"
     CDS             <1..698
                     /locus_tag="PLI69_04495"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG41781.1"
                     /translation="SKKVEKICVAYSGMEKFFPSEKIILTGNPIRKEIKPSTAAEREE
                     GYDFYGLNQDKKTIFVVGGSLGSGTLNKAMKKWVAEKGANADYQVIWQNGKYYSSDIT
                     KYMKKRELPHVKHFDFIRRMDLAYAVADVVISRAGAGTISELCVAGKATIFVPSPNVT
                     EDHQTHNAMALVEKEAALMVRDDMAEEELMDTAEKLLSDSEKIRKLEKNVLKLGRKGA
                     AEAIATEVLSLIK"
     gene            708..2078
                     /gene="murC"
                     /locus_tag="PLI69_04500"
     CDS             708..2078
                     /gene="murC"
                     /locus_tag="PLI69_04500"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOG41782.1"
                     /translation="MGKKYKNYYFIGIGGIGMSAIARYFHRTGHRVSGYDRTPSALTA
                     KLEKEGIAVHYEDDISAVPADKENTFVIYTPAVPADMDELKYVKEQGYTLYKRSQALG
                     EIARGQKCLAVAGTHGKTTTSTLLAHILTDSGEGCNAFLGGISRNYDSNLLMSSNQVL
                     VAEADEFDRSFLQLYPDIAVITSADADHLDIYSDHSHVREAFSQFASQVKSDGFLVLK
                     KGVKISLKGVSAKILSYSYDSPADFYASDIVPLDGGFYDFTLNTPEGKIEHCTLGIRG
                     WVNVENAVAASAIALIHGTPPEKLKKALASFKGVGRRFDIRINTPQYAYVDDYAHHPN
                     ELRHAISSLRGIFKGRRLCGIFQPHLYSRTRDFVDEFADALGGLDELILLHIYPAREL
                     PIEGVTSKIIFDKVKLENKILIGKDELMETIRKRDFDCLITFGAGDIDRFVLPIEEEL
                     KARIKK"
     gene            2075..2848
                     /locus_tag="PLI69_04505"
     CDS             2075..2848
                     /locus_tag="PLI69_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41783.1"
                     /translation="MRVKKILLVVLISLLFLGVVVPYFYFVAEMEKAAIAETRCSGIN
                     VNFIDSLKVNLISREEIISLVEQAEKVMGTNYEKVNLKRIETLLDNRGEILKSEVYFS
                     SPTILSIDIVQRKPVVRFENETEGYYADGSGFLFPLLNSYNVPIVTGDIPLRLGREYM
                     GIPEGEESEWVNGIIRLTDYISSNEYWNKNIEQIYIENGDICIVSHVSHHKIIFGNFR
                     NIDAKFKKLATFYKTIAPSEGWNTYDVVNLKYNKQIICK"
     gene            2857..>3969
                     /gene="ftsA"
                     /locus_tag="PLI69_04510"
     CDS             2857..>3969
                     /gene="ftsA"
                     /locus_tag="PLI69_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOG41784.1"
                     /translation="MSNFITAIDFGSSKVAVAVGTKSADGIKVVSFHSHPLPNGIKNG
                     EIINEGRVIEALKAALGAASSDIGEEINKAVINISGKILFRKEIESTIIRNLSSKYVS
                     AEELEGYRRERYKYSVDEGDMIYAVLPQKYDIDDLIGYQQHEIIGTSGKNLKAYYKIF
                     YGKAALLSRCKKVLSACGVEISKIVLSPIASARAVLTEAEMENGVMLVDIGRGLTQIA
                     VVRKGIVTDVRIIPFGGESITVDIQNVTSISPKWAELIKLKHGCCVEEFTPEDKKLVL
                     RGNDGIIEDEVSLIGLTRIIEARTSEILEAIKYIKEENESQGKLSAGVVITGGTCYLE
                     GFLDLAKVFLGKKTRLAAPRGCITIDSHEKAQDTFSS"
BASE COUNT         1232 a          781 c          970 g          986 t
ORIGIN      
        1 tatccaagaa ggtggaaaaa atttgcgtag cctatagtgg aatggagaag tttttccctt
       61 cggaaaaaat tatacttacg ggaaatccca tcagaaagga gataaaacca tctacggcgg
      121 ctgaaagaga ggaggggtac gatttctacg ggcttaatca ggataaaaaa accatctttg
      181 tagttggtgg cagcctgggc agcggaactc tcaacaaggc aatgaagaaa tgggtggctg
      241 aaaagggtgc aaatgcagac tatcaggtaa tatggcaaaa tgggaagtat tatagctccg
      301 atattaccaa atacatgaaa aaaagggaac tgccccatgt aaagcatttt gatttcattc
      361 gaagaatgga tctggcctat gctgtggccg atgtggttat ttcccgcgca ggggccggca
      421 ctatctccga actctgcgtt gccggcaaag ccaccatatt cgtcccgtca ccaaatgtga
      481 cggaagatca ccagacacac aatgccatgg ctttggtaga aaaagaggct gcgctgatgg
      541 ttagagatga tatggctgaa gaggaattga tggatactgc cgaaaaactt ctctcagata
      601 gcgaaaaaat ccgcaaactg gaaaagaatg tgctaaaact gggacgcaag ggagctgcag
      661 aagcgattgc aacagaggta ctctccctca taaaataaga gatgaaaatg ggaaaaaaat
      721 ataaaaacta ctacttcata gggattggtg gcattggcat gagtgccatt gcacgctact
      781 tccatcgtac aggtcacaga gtttccggtt acgacaggac tccctccgca ctcaccgcaa
      841 aactcgagaa agagggcatt gccgtacatt atgaggatga tattagtgcc gttccggcag
      901 acaaagagaa tacctttgtc atctataccc cggccgttcc ggcagatatg gacgaactta
      961 aatatgtgaa ggagcaggga tacactttgt acaagaggtc ccaggcattg ggtgagattg
     1021 ctcgcgggca gaagtgtctg gcagtagccg gaacacatgg taaaaccacc accagtactc
     1081 tactggccca tattcttaca gactccggag agggttgcaa cgcttttttg ggtggtattt
     1141 cccgcaatta tgactccaat ctgctaatga gttccaacca ggtgctggtg gcagaggccg
     1201 acgagtttga tagatccttc ctacaattat accccgatat tgcggtgatc acctcagccg
     1261 atgccgacca tttggatatc tactcagacc acagtcatgt gcgggaggca ttctcccaat
     1321 ttgcttccca ggtcaagagt gatggattcc ttgtgcttaa gaaaggagtg aaaatctcac
     1381 tgaagggagt aagcgcaaag attttaagct actcatacga ctctcctgct gacttttacg
     1441 catcggacat tgtgcctctc gacggtggtt tctatgattt tacactcaat actcccgaag
     1501 gaaagataga gcactgcaca ttgggcatac gggggtgggt aaatgtagag aatgctgtgg
     1561 ctgcctctgc aattgctctc atacacggca cgcctcccga aaaacttaaa aaagcgctag
     1621 caagctttaa aggggtgggc agaagatttg atatccgcat aaatactccg cagtatgcct
     1681 atgttgacga ttacgcgcat caccccaatg aattacgcca tgccatcagt tctttgagag
     1741 gtatttttaa gggaaggagg ctgtgcggca tattccagcc ccatctctat tctcgtacaa
     1801 gggatttcgt tgatgaattt gcagatgcgt tagggggact ggatgagctg atattactac
     1861 atatatatcc tgccagggag cttccgatag aaggggtgac ctctaagatt atctttgata
     1921 aggtaaaact tgaaaacaag atactcatag gtaaggatga attgatggag actatccgta
     1981 aaagagattt tgactgcttg ataacctttg gagcgggaga cattgacaga tttgtattgc
     2041 ccatagagga agaattaaag gcaagaataa agaaatgaga gttaaaaaga tattgctggt
     2101 ggtactgata tcccttctat ttctgggagt ggtagtgcca tacttttatt ttgtagctga
     2161 aatggagaaa gcggccatag cagagacgag atgcagcggt atcaatgtaa attttataga
     2221 ttcccttaag gtaaatctta tcagcagaga agagattatt tccctggtgg aacaggcaga
     2281 gaaggtgatg ggtacaaatt atgaaaaagt taaccttaag cgtatcgaaa ctttgctgga
     2341 caacagaggc gagatattaa agtccgaggt ctatttttcc tcccccacta ttctaagtat
     2401 agatatagtc cagaggaaac ctgttgtaag gtttgaaaat gagactgaag gctattatgc
     2461 ggatggtagc ggttttctgt tccccttgct aaatagctac aatgtgccta ttgttacggg
     2521 tgatatacct ctgcgtctgg gcagagagta tatgggcatt cccgaagggg aagagagtga
     2581 atgggtaaac ggtattattc gcctgactga ctatatcagc agtaatgagt actggaataa
     2641 aaacattgag cagatatata ttgaaaatgg ggatatctgt attgtttcac atgtaagcca
     2701 tcataaaatc atattcggca acttccgtaa tatagatgct aaatttaaaa aattggcgac
     2761 attctataaa acaattgctc cgtcagaggg atggaatact tatgatgtcg tcaatctcaa
     2821 atacaacaaa cagattattt gcaaataaaa agatatatga gcaattttat tacagctata
     2881 gacttcggat catctaaagt tgccgtggca gtaggcacca agagtgccga tggcataaag
     2941 gtggtctctt ttcacagtca tccccttcct aacggcataa aaaacgggga gataataaat
     3001 gagggcaggg ttatcgaagc cctcaaagcg gcacttggtg ctgcatcatc cgatattgga
     3061 gaagagatca ataaggccgt gatcaatata agcggaaaaa ttctcttccg taaggagata
     3121 gaaagcacaa tcatcaggaa tttaagcagc aaatatgttt ccgctgaaga actggaaggt
     3181 taccgccgtg agcgttataa atattccgta gacgaagggg atatgattta tgcagtcctc
     3241 cctcagaaat acgatatcga tgatctgata ggataccaac aacatgaaat cataggaacc
     3301 tcgggaaaga accttaaggc ttactacaaa atcttctacg gcaaagcagc attgctctcc
     3361 agatgcaaaa aggtactctc tgcatgtggt gtagagatat ccaaaatcgt actctcgccc
     3421 attgcctccg ccagagcggt gctaactgaa gcggagatgg aaaacggagt gatgctggtg
     3481 gatattggac gaggacttac acagattgca gttgtaagaa aaggcattgt aaccgatgtg
     3541 cgaatcatcc cctttggagg agagtcaatt acggtagata tacagaacgt aacctcgata
     3601 agtcccaaat gggccgaatt aatcaagctg aaacatggat gctgtgttga agagtttact
     3661 cccgaggaca aaaaactggt actacggggc aatgacggca taatagagga tgaagtgtcc
     3721 cttataggac tcacccgcat tatcgaagcg cgcaccagtg aaattctgga agcgatcaag
     3781 tatataaagg aagagaatga atcgcaagga aaactctcgg ccggagttgt tataaccggc
     3841 ggtacctgct atctggaagg ttttctggac cttgcaaagg tttttctggg caaaaaaacg
     3901 cgtcttgctg cacccagagg ctgtataact atagattcgc atgagaaagc acaagatacc
     3961 ttctcttcc
//
LOCUS       DAOSIE010000122         3953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_20165_length_3953_cov_3.442022, whole genome shotgun sequence.
ACCESSION   DAOSIE010000122 DAOSIE010000000
VERSION     DAOSIE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20165_length_3953_cov_3.442022"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLI69_04515"
     CDS             <1..308
                     /locus_tag="PLI69_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41785.1"
                     /translation="VLADFYLESGRFSFFTGATAGRGARKNLLIFEGSKSDWIAEESV
                     FFHENSFFGIDPFIGAEFAVTKSIHLVFRVDYLMQFNNKMLSDLVGPRAFIGIMFCR"
     gene            complement(324..1514)
                     /locus_tag="PLI69_04520"
     CDS             complement(324..1514)
                     /locus_tag="PLI69_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOG41786.1"
                     /translation="MEFNLFHNLLEQWGVGERLIHEFELLFFAVAVTLLILLINLISR
                     RVVIPIIKRVVKKTRNKFDDILLDEKFLKNSVRTLTPIIILIFIPYAVDSFPVLMEVL
                     KRAAAVWLTISITVLICSFFEAIYTYLSAKEVAQQHPLKGFIQMFKIIVICIGLIVIM
                     SIIVNKNPAAILTALGASAAVLMLVFKDTIVGLVAGIQLSANDMLRPGDWISMPKYGV
                     DGDVIEVSLTTVKVENFDKTISTIPPYALISDSFQNWRGMKETGGRRVKRHIPIDMNT
                     IGYLSKEQREDLVSRGWMSADASTDIVNLKVFRDYLENYLRTHTKVHKELTLLVRELQ
                     PTSEGIPLELYFFSNTTVWAEYETIQSEVIEHVLSVMPKFGLRAFQRSSGSLSSLENP
                     IKYS"
     gene            1597..1992
                     /locus_tag="PLI69_04525"
     CDS             1597..1992
                     /locus_tag="PLI69_04525"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOG41787.1"
                     /translation="MEKRKIEVVAAVIFDEKKILVSQRDGGDMAGRWEFPGGKIEAGE
                     THEQALKREIMEELGIIISVDSFLLTIEYDYPEFHLVMHCYQCAILFGAPRLLTHSAV
                     KWISPQELDNIDWLPADIQVVDYLKKHLF"
     gene            2050..3357
                     /gene="guaA"
                     /locus_tag="PLI69_04530"
     CDS             2050..3357
                     /gene="guaA"
                     /locus_tag="PLI69_04530"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOG41788.1"
                     /translation="MKQDMIVILDLGSHENTVVARAIRAMGVYSEIYPHDITAKELKE
                     LPNVKGIIINGGPNNIIDGVEIDVLPEIYRAGIPVMAAGHDKALCDVKLDEFTSDESA
                     IIKALKDFIFDTCKAEANWNMANFVADQVALVHKQVGDRKVLLALSGGVDSSVVAALL
                     LKAIGDRLVCVHVNNGLMRKNESENVVELFRNRLGANLIYVDATDRFLSKLEKVADPE
                     QKRKIIGTEFIRIFEEEARKLKGIDFLAQGTIYPDIIESGTKTAKCVKSHHNVGGLPE
                     DLQFELVEPLKHLFKDEVRACSLELGLPHEMVFRQPFPGPGLGVRCLGAITRDRLEAV
                     RESDAILREEFAKAGLDKKVWQYFTVVPDFKSVGVRNNERSFDWPVIIRAVNTVDAMT
                     ATIEPLDWDFLKHVTERILNEVPHINRVCYDISPKPSATIEWE"
     gene            3368..3556
                     /locus_tag="PLI69_04535"
     CDS             3368..3556
                     /locus_tag="PLI69_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41789.1"
                     /translation="MKKEEFIRFAMEAHRMSCLFLHGNQCRTGAREARPCDRGCSYMI
                     DFETRLRDLCQPRIDFGD"
     gene            complement(3567..>3953)
                     /locus_tag="PLI69_04540"
     CDS             complement(3567..>3953)
                     /locus_tag="PLI69_04540"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG41790.1"
                     /translation="LYVEDHARAIDLIFHRGEVGETYNIGGFNEWKNIDLIKVIIATV
                     DRLLGNPEGYSLDLITFVKDRAGHDLRYAIDSRKLQKELGWEPSLQFEEGIELTVRWY
                     LDNQEWMDRITSGDYERYYEEMYHKR"
BASE COUNT         1132 a          857 c          886 g         1078 t
ORIGIN      
        1 gagtcctggc agacttctat ttggagagcg gtaggttctc cttttttacg ggagcgaccg
       61 caggaagggg ggcacgtaaa aatcttctga tattcgaagg atcgaagtcg gattggatag
      121 cagaagagag cgtttttttc catgaaaatt ctttttttgg aatagatccc ttcataggag
      181 ctgaatttgc cgttacaaaa tcgatacact tagtatttcg tgttgattat ctcatgcagt
      241 tcaataataa aatgctctcg gatcttgtag gaccgagagc atttatagga atcatgtttt
      301 gccgttgagg cagaaatcat ttcttaagaa tacttgatag ggttctccag gcttgaaagt
      361 gaacctgaac tacgctggaa ggctctcaga ccaaactttg gcattaccga aagtacatgt
      421 tcgataacct ccgactgaat agtttcatat tctgcccaga cggtggtgtt tgaaaagaaa
      481 taaagctcca atgggatacc ctcagaagta ggctgcaatt cccttacaag caaagtaagc
      541 tctttatgca ctttggtatg agtgcggaga taattctcca gatagtctct aaatacttta
      601 agattgacaa tatcggtaga tgcatcagca gacatccatc ctcttgaaac caaatcctcc
      661 ctctgctctt tcgagaggta tccgattgta ttcatatcta tgggaatgtg tctctttaca
      721 cgtctgcccc ccgtttcctt catccctctc cagttttgga aggagtcgct aataagtgca
      781 taaggaggga ttgtggagat agtcttgtca aaattctcta ccttgacagt tgtaagtgaa
      841 acctctatta catcaccatc tacaccatat ttgggcattg agatccagtc tccggggcga
      901 agcatgtcgt tggctgagag ttgaataccg gccaccaatc caacaatggt atccttgaaa
      961 accaacataa gtactgcagc agaggcgcca agggcagtca ggattgccgc gggattcttg
     1021 tttactataa tactcataat gacaatcagc cctatgcaga tcactatgat cttgaacatc
     1081 tggataaaac ccttcaaagg gtgctgttga gccacttctt tagcactgag ataggtatat
     1141 attgcctcaa aaaaggagca aataagtact gttatggaaa tggtcagcca tacggcagca
     1201 gccctcttta agacctccat aagcaccgga aaggaatcga ctgcgtaggg tatgaaaatc
     1261 aatatgataa taggtgtcag agtcctgact gagttcttaa gaaacttctc atccaataaa
     1321 atatcgtcga atttattacg agtctttttg acgacacgct ttatgatggg tatgacaacc
     1381 cttcgtgata tgagattgat aaggaggatc aggagcgtta ccgccacagc aaaaaagagg
     1441 agctcaaatt catggattaa ccgttccccc actccccatt gttcaagcaa attgtgaaat
     1501 aaattaaatt ccatattatc aattatcttt tgttgaccct gcaaaaatag gaaaatatgc
     1561 cgaaattctt aattttgtac gatttaagtt ttcaatatgg agaaacgaaa aatagaagtt
     1621 gttgctgccg tcatatttga cgagaaaaaa atattggttt cacaacgtga cggtggagac
     1681 atggccggtc gttgggaatt tcccggtgga aaaattgagg caggtgagac acatgaacaa
     1741 gcccttaaac gggagataat ggaggaactg gggataataa tctccgtaga ctcatttctg
     1801 ctcacaatag agtatgacta ccctgaattt caccttgtaa tgcattgtta ccaatgcgca
     1861 attttatttg gagctccgcg cctactgaca cacagtgcag taaagtggat aagccctcag
     1921 gaactcgata acattgactg gcttcccgcc gacatacaag ttgtagatta tctgaaaaag
     1981 catctatttt aagggctaat aatgtatctt tgcaactgat ttcctaaatc aatgcttaat
     2041 atccgaagaa tgaaacagga catgattgta atactagacc tcggcagtca cgaaaacacg
     2101 gtggtggcca gggccatccg tgcaatgggt gtttatagtg agatttaccc ccatgacatc
     2161 acggcaaagg aactaaagga actcccaaat gtaaaaggca taatcatcaa cggtggcccc
     2221 aataacatca tagatggtgt tgagatcgat gtcctgccgg agatatatag ggccggaatt
     2281 cccgttatgg cagccgggca tgataaagcc ctctgcgatg ttaagcttga cgagttcaca
     2341 tctgatgagt ctgccatcat aaaagccctg aaggacttta tattcgacac ctgcaaagct
     2401 gaagccaatt ggaatatggc aaatttcgtg gcggatcaag tagcattggt tcacaagcag
     2461 gtaggtgacc gtaaagtact gcttgcactc tccggagggg tcgacagttc cgtggttgca
     2521 gcacttctgt taaaagccat cggcgaccgt ctggtgtgcg tgcatgtcaa caatggcctt
     2581 atgaggaaaa acgagtccga aaatgtagta gaacttttcc gaaatcgtct cggtgctaat
     2641 ctgatatatg ttgatgctac cgatagattt ctttcaaaac tggaaaaggt agcagatcct
     2701 gaacagaaaa gaaagattat aggcactgaa tttataagga tatttgaaga agaggctcgc
     2761 aagctcaaag gaatagactt cctggctcag ggtaccatat atcccgatat tatagaatcg
     2821 ggtaccaaga ctgccaaatg tgtcaaatct catcacaacg tgggcggcct ccctgaagat
     2881 ttacaatttg agctagtaga gccgcttaaa catctgttta aagatgaagt acgcgcctgc
     2941 agtcttgaat tgggtcttcc acacgaaatg gtcttccgtc agcctttccc cggccccggt
     3001 ctgggagtac gatgtcttgg agccattaca agagatcgtc tcgaggctgt tcgcgaatcc
     3061 gatgcaatat tgcgtgaaga gtttgccaaa gcaggacttg acaagaaggt atggcaatat
     3121 tttacagttg tcccggactt taaatcggtc ggagtacgca acaacgaacg ttcatttgac
     3181 tggcctgtga ttatccgtgc cgtaaacact gtcgatgcca tgactgccac catagagccg
     3241 cttgattggg attttctgaa gcacgtgact gagcgtattc tcaatgaagt cccccatatc
     3301 aaccgtgtct gctacgatat ttcacctaaa ccctcggcca ctattgagtg ggaataagcc
     3361 ttctactatg aaaaaggaag aatttatcag atttgccatg gaagctcata ggatgagctg
     3421 cctctttctt catggtaacc aatgcaggac aggcgcacgc gaagcacgtc cttgtgatcg
     3481 cggatgttcc tatatgatcg actttgaaac acggctgcgg gacctctgtc agccgcgtat
     3541 tgattttgga gactagaccc tgttccctat cttttgtgat acatctcctc gtaatagcgc
     3601 tcatagtcgc ctgaggtgat cctgtccatc cattcctgat tgtccaggta ccagcgtaca
     3661 gtcagttcta taccctcttc aaattgcagt gaaggttccc atcccaactc tttttgtagt
     3721 ttcctggaat ctatggcata acgcaaatcg tgacctgcac ggtctttgac gaatgtaata
     3781 aggtcaagcg aatatccttc gggattaccc agcagacgat ccacagttgc gattatgacc
     3841 tttatcaggt ctatgttttt ccactcattg aaaccaccta tattgtaggt ctctcccacc
     3901 tcaccgcggt ggaaaatcag gtcaatagcc ctggcgtgat cttcaacgta gag
//
LOCUS       DAOSIE010000123         3953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_20167_length_3953_cov_3.048743, whole genome shotgun sequence.
ACCESSION   DAOSIE010000123 DAOSIE010000000
VERSION     DAOSIE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20167_length_3953_cov_3.048743"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="PLI69_04545"
     CDS             complement(<1..1706)
                     /locus_tag="PLI69_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41791.1"
                     /translation="MKKIIYSLVIMIAASTLFTSCLEYVEPVGIQQLRTAKADYLDAL
                     AQLRLADAELQKANATFVLARAAFQDAQTEWQLIENRIHEYDVQIKAARTQYEVDFWT
                     KEKEHLQITHNTRMAQARQNLANAEENLRVALRNIAAVQNLFTDQERIIFARVLNDYE
                     TAFNNYNAKLFGLEQAQAKLWELEYAFKDSTDWEANYQGEIDFFTNEIARAQAALNAI
                     PKKLDLEAWKAEVDNLQDSIDAYNYSRQAIIKDSVQYMVNVYHEGAPCYERKLDAWEE
                     ANKVTVMLDAEKWVFKELKDVTPNKPVKPVAPVYSSADARIQWNFVRDYTDAAAKLVY
                     TKFYDLIADYFQNAPFGKDSTTANGGYYANYIAGVVVPNMDTIRINATVDMKDFILND
                     STPTPRTYKWKKGNVENTSSGKYGLKGAINILERELVLIDAASNVAAKQTAYNNARNR
                     WLADREYLINKTHLVEEAAALANMKTSVVIPTPGANDGRAEDLIYAIKDFRGSRTADG
                     TNSSYADTMGLINAIKDFFNAKKAYIGNPFKDFDTITFKNAMGEDAKVFLACVAFHSP
                     HRV"
     gene            complement(1864..3927)
                     /locus_tag="PLI69_04550"
     CDS             complement(1864..3927)
                     /locus_tag="PLI69_04550"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOG41792.1"
                     /translation="MAVLEKIRVKLGILITVLIAVALLSFIIDPQTLSSTAQRFSSDN
                     KVGVLDGKSISYSDFYLEVEYYTRLTELMGQGSNSEEAQKAIRNAAWQSLLDKHVFQP
                     MIADAGISVGDEEMLDLTQGSNISQVVANQQMFQDANGLFSREALVSFVQAIPTDATG
                     NYEAYWNSVENSVYTDRLYNKYGALIEASNIYNKVEQQRMMIENNVLSSVDFVLSPLG
                     FERDTTITVTPKEIKAYYDARKELFTRPASRNIEYVLYEVVPSQEDIETQTETFHALY
                     AEFAVADNPKNFIALNSDRKWDGYYYTEEQLESEPHFKAIAFGKNAPAVSEISIGESQ
                     LAAARLYDVKVMADSAKVWYAAFPLDNEGEADALLAKAQKMKEPSEEFTEMGWITQEY
                     FSAPGMEGFEKALEPAAGKAIKIKSIGANAYFVLYVGERTKAQRRAQLATFVKNIVPS
                     EETYRDFQMKATDLADRADGDYDKFAKIVREEQLPVIPVNGLLQTTERIGVVDNARSV
                     VYWAFDRKTRSGDVSDVISVDNKYYFVAAVTAAYKEGRIPLSDIQQDIVYTITAEKQI
                     EKMASAIGEKIEGCTTMEQVAEVLGTTVSHQDGITFGSTLQQLDPKFIGAVTSAQPGV
                     ISGPVAGDIGVYVFQVNDRQTGTFYTDGDMQRALARKSAYQTQLLQQVMSEIVEIKDN
                     RARFF"
BASE COUNT          974 a          936 c          922 g         1121 t
ORIGIN      
        1 actcggtgcg gggagtggaa ggcgacgcag gcaaggaata ctttggcgtc ttcgcccatg
       61 gcattcttaa aggtgatggt gtcaaaatcc ttgaagggat tgccgatata agcttttttc
      121 gcgttgaaga aatcctttat ggcattgata agacccatgg tgtcagcata cgagctattt
      181 gtaccatcag cagtacgact acccctgaag tctttgatag cgtagatcaa atcctcagcc
      241 ctgccgtcat tagcaccggg ggtagggatc actacgctgg tcttcatgtt ggcaagagca
      301 gctgcctctt ctacaaggtg agtcttgttg atgaggtact cacggtcggc aagccacctg
      361 ttcctcgcat tattatatgc tgtctgctta gcagcaacgt ttgaagctgc atcgatcaat
      421 acaagctctc tttcgagaat gttgatggca cccttgagac catactttcc gcttgaagtg
      481 ttttcaacat tacccttctt ccacttatat gttctcggag tgggagttga gtcgttgagg
      541 atgaaatcct tcatgtcaac ggtagcattg atcctgatgg tatccatgtt cggtacaact
      601 acaccggcta tataattggc atagtaaccg ccgtttgctg tagtagagtc ctttccaaaa
      661 ggagcgttct gaaagtaatc ggctataagg tcataaaact ttgtataaac gagctttgca
      721 gcagcatcgg tataatctct gacaaagttc cattggatac gggcatccgc acttgaataa
      781 acgggagcta ccggtttaac gggcttatta ggagttacat ctttgagctc tttaaacacc
      841 cacttttcgg cgtctagcat aaccgtaacc ttatttgcct cttcccaagc atcgagctta
      901 cgctcgtagc aaggagcacc ttcatgatat acgtttacca tatattgaac ggagtccttg
      961 ataatggcct gacgtgagta gttgtatgca tcgatggagt cttgcagatt gtcaacctca
     1021 gccttccagg cctcgaggtc gagcttcttg ggtatggcat tgagagcagc ctgtgccctt
     1081 gcaatctcat tggtgaagaa gtcgatctct ccctgatagt tggcttccca gtcggtagag
     1141 tctttgaaag catactcaag ctcccagagc tttgcctgtg cctgctcgag tccgaaaagc
     1201 tttgcattgt agttattgaa agcagtctcg tagtcattga gaacacgggc gaagattata
     1261 cgctcttgat ctgtgaaaag attttgaacg gcagcaatat tgcgaagtgc tactctgagg
     1321 ttctcttcag catttgccaa attctgtctg gcctgcgcca ttcttgtgtt gtgggtaatc
     1381 tgaagatgtt ctttctcttt cgtccaaaag tcaacttcgt actgcgttct agcagctttg
     1441 atctgaacgt catactcgtg aattctgttc tcgatcagtt gccactctgt ctgagcatcc
     1501 tggaaagcag cgcgagccaa cacaaaggta gcattggctt tttgcaactc ggcatctgcc
     1561 aagcgtagtt gtgcgagtgc atcgagatag tcggcctttg cagtgcgaag ctgctggata
     1621 ccaaccggct caacatactc aagacacgaa gtgaaaagag tgcttgcagc aatcatgatc
     1681 actaatgaat aaataatctt tttcataaaa aaaaattgtt aagtgtttga tatttattgg
     1741 ttaaacataa atgaattgcc gaaaaaacac agtttttcca tagtatttaa tggccaaatt
     1801 tagttatatt tttttacttt tcaaaaaaaa ttgttaataa ttatcaaaaa atgttgaaaa
     1861 aatctagaaa aatcgggctc tgttgtcctt gatttcgaca atttccgaca ttacctgctg
     1921 taacaactgg gtctgatatg ccgatttgcg cgccagcgct ctctgcatat ctccgtcggt
     1981 atagaaggta cctgtctgac ggtcgttaac ctggaataca tatactccga tgtcccctgc
     2041 aaccggcccg ctgatgactc ccggctgagc ggaggtcact gcgcctatga acttagggtc
     2101 gagctgctgg agtgtggaac cgaaggtaat gccatcctga tggctcacgg tagtgccgag
     2161 cacctcggcc acctgctcca tagttgtgca accttcaatc ttctcaccga tggcagaagc
     2221 catcttttcg atttgcttct cagcggtgat ggtatagaca atatcctgtt gtatgtcgct
     2281 cagaggtatt ctgccctctt tatatgcagc ggtcacggca gcgacgaaat agtatttgtt
     2341 gtcaacggaa atcacgtccg atacgtcgcc cgacctggtc ttacggtcaa atgcccagta
     2401 gactacgctg cgcgcattgt ccacgactcc gatacgctcc gtggtctgaa gcagaccgtt
     2461 gacggggatg acgggaagct gctcctcccg tacaatcttt gcgaacttat cgtaatcacc
     2521 atcagctctg tccgcaaggt cggtagcctt catctggaag tcacgatagg tctcttcgga
     2581 gggaactatg ttctttacaa aagtggcaag ctgagccctt cgctgagcct tggtacgctc
     2641 acctacatag agcacgaaat aggcattggc accgatgctc tttatcttga tggccttgcc
     2701 ggcagcaggc tcgagagcct tctcaaatcc ctccataccc ggagccgaaa agtactcctg
     2761 agtaatccat cccatctcgg taaactcctc cgagggctct ttcatcttct gagcctttgc
     2821 aagcagtgcg tcggcttcgc cctcattgtc cagagggaag gcagcatacc acacctttgc
     2881 agagtcggcc atcaccttta catcatacag acgggctgct gcaagttgac tttctccgat
     2941 actgatctcg ctcactgcgg gagcattctt tccaaaggct atagccttga agtgaggctc
     3001 ggactcgagc tgctcttcag tatagtaata gccatcccac ttacgatccg agttgagagc
     3061 tataaagttt ttaggattat cggctaccgc aaattcggcg taaagcgcat ggaaggtctc
     3121 ggtctgagtc tcgatatctt cctgtgaggg tacgacttca tagagcacat actctatatt
     3181 acgggaggcc ggacgggtaa atagctcctt gcgtgcatca tagtaggcct ttatctcctt
     3241 gggagtaacg gtgatagtgg tatcgcgctc aaagccgaga ggagagagta cgaaatccac
     3301 tgaagagagg acattgttct ctatcatcat acgctgttgc tctaccttgt tgtaaatatt
     3361 ggatgcctca atcaatgctc catatttatt gtaaagccgg tcggtgtaaa cggaattttc
     3421 cacggaattc caataggcct cataattacc tgtagcgtcg gtggggatag cctgtacaaa
     3481 gctgacaaga gcctcacggc tgaataggcc gttggcatcc tgaaacattt gctgatttgc
     3541 caccacctgg gagatgttgg acccctgtgt aaggtcgagc atctcttcgt ctcccacgga
     3601 tatgcctgca tctgcaatca tgggctggaa aacgtgcttg tcgaggagag actgccatgc
     3661 cgcattgcgg atggcttttt gtgcctcttc actgttggaa ccctgtccca tcaactcagt
     3721 cagtctggta tagtactcta cttccaggta aaaatcacta tatgaaatgg acttgccgtc
     3781 caatacacct actttattgt cggatgaaaa tctttgtgca gtcgaactca atgtttgagg
     3841 atcaatgatg aatgataaca gcgcgactgc gataagaact gttataagta tacctaattt
     3901 gacgcgaatt ttctcaagaa ccgccatttt gtttgttaag ttttagttta ttt
//
LOCUS       DAOSIE010000124         3949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_20190_length_3949_cov_4.217001, whole genome shotgun sequence.
ACCESSION   DAOSIE010000124 DAOSIE010000000
VERSION     DAOSIE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20190_length_3949_cov_4.217001"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLI69_04555"
     CDS             complement(<1..358)
                     /locus_tag="PLI69_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41793.1"
                     /translation="MLNNSTIRKFRRVAAALPALFVLFTAIPSAAQKVAADGGFTPYS
                     MFGVGFIAKQGTSYNLSMGGIGIGDRNFRYINYLNPAAVTERQAQSFMLDFGMEEGNV
                     YYAANPATAVGGAGSDL"
     gene            complement(345..1151)
                     /locus_tag="PLI69_04560"
     CDS             complement(345..1151)
                     /locus_tag="PLI69_04560"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOG41794.1"
                     /translation="MDKMYLLIDIGNTACKVAFADNEKIGKVYRCAEEDIRQFIRDVF
                     AAEHPGQRADVAVLSNVRADDPALEEMLRNERCNRLIVLDVPECNRLVETGLVQFEVL
                     ANMPEGMGADRIAAILAANNLFPENDLMVFDFGTAITVEFINCHKEEAPASYVGGAIS
                     LGLNTRYRALGHFAERIQLLNPRDFIESHKEEKISPIGFDLESAMAAGNILGIMFEIE
                     GYRREYPERTVIFTGGDAIYFAEKLKSSIFVVHNLVLMGLSQVAQFYAEQ"
     gene            complement(1193..>3949)
                     /gene="mfd"
                     /locus_tag="PLI69_04565"
     CDS             complement(1193..>3949)
                     /gene="mfd"
                     /locus_tag="PLI69_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOG41795.1"
                     /translation="KKSEIRRTILSIKKGDRLSHEFIKEVLYESGFEKVDFTSEPGQF
                     ALRGGIIDIFSFADNLPVRIDFFGDQVESIRKFDINTQLSKESLESVDIFPDIHKGID
                     ESSLVPFWEILPENAVLWDLRGVNPTGENDVIPQPTFNKNFEILSQDIRSKMDQGYGV
                     TILSPNEAQTDRLRQILVDTGEKVRPDFLPLTLHEGYVDRISKNCYYTDHQIFERYHK
                     IKVKRQVERSERLTINEIMAFQMGDYVVHIDYGVGKFGGLVKTNVNGKVQEAIKLVYK
                     DNDVVFVSIHGIHRISKYKSRDGEPPKIYKLGTKAWENLKSRTKSKVKDIVDDLIKLY
                     SARQHSKGFAFSADNYLMHELESSFMYEDTPDQALASKKVKEDMESDRPMDRLVCGDV
                     GFGKTEVAIRAAFKAVCDSKQVAVLVPTTILALQHYHTFSSRLKDFPCRVEYISRLRN
                     TKEISRILEELRAGRVDILIGTHRILNSKVLFKDLGLLIIDEEQKFGVSSKEKIRQMR
                     SNVDTLTLTATPIPRTLQFSLLGARDLSIINTPPPNRKPVYTEVINFKDELITDIINY
                     ELERGGQVFFVHNKIEDILSVRDILQRLLPEAQICVAHGQMEPEVLEKRIVEFIAGEH
                     DILLATTIIENGIDIPNANTMIINQAQRFGLSDLHQLRGRVGRSNVKAYCYLLIPPIA
                     SITEDARRRIRAIEAFSDLGSGFNIAMQDLDIRGAGNLLGAEQSGFIAEMGFETYMRI
                     LNEAMSELREERIGRGEEMVMPDTMQSAPMVDTQIETDLEAYIPDEYVQVASEKIRLY
                     KDLTNIGDERQIEALRREMEDRFGPIPGQLEELIRIVHLKRVAQNLGFEKIILKNGVM
                     LAYFISNSMHSYYRGDLFPKILTAIQSLGDRCRVNEVKEKLYIRVKSIPNVSQAILFL
                     SKLI"
BASE COUNT          957 a         1001 c          878 g         1113 t
ORIGIN      
        1 gcagatcgga acctgcccct ccaaccgccg tggccggatt tgccgcataa tagacgtttc
       61 cctcttccat gccgaagtcg agcatgaagg attgcgcctg gcgctccgtc acagccgcag
      121 gattgagata attgatatat cggaaattgc ggtcgccgat accgatgcct cccatagaga
      181 ggttgtatga ggtgccctgt tttgcaataa aacctactcc aaacatggag taaggggtaa
      241 aaccgccgtc ggcagcaact ttctgcgccg cagaagggat ggctgtaaaa agcacgaata
      301 gagcaggcaa ggctgccgcg actcttctga atttccgtat ggtactattg ttcagcataa
      361 aattgtgcta cctgtgagag ccccatcaaa acaaggttat gaacgacaaa tatggaactt
      421 ttaagttttt cagcaaaata aatcgcatcc cctccggtga aaatcaccgt tcgttcagga
      481 tactctctcc tatatccttc tatctcaaac attatgccga gaatatttcc cgccgccatg
      541 gcactctcca gatcgaaacc tatgggagat attttctcct ccttgtgaga ctctataaag
      601 tccctcggat tgagtagctg aatacgctcc gcaaagtgtc ccaaagccct ataccgcgta
      661 ttcaaaccca aggaaatagc gcctcccaca taagatgccg gagcctcctc tttatggcaa
      721 ttgataaatt ccacagtaat ggcggtaccg aaatcaaaaa ccataagatc attttctgga
      781 aagagattgt tagcagccaa aatagccgcg atacgatctg ctcccatgcc ttcaggcata
      841 ttggcaagca cctcaaactg cacgagtccc gtctccacca gacgattgca ttcgggtacg
      901 tccagcacga tcaaacgatt gcaacgctcg tttctcagca tctcctcaag agcgggatcg
      961 tcggcgcgaa cattacttaa aacggccaca tccgctcttt ggcccggatg ttccgctgca
     1021 aaaacatcac ggataaactg tcggatatcc tcttccgcgc agcggtaaac cttccctatc
     1081 ttttcattgt cggcaaaagc caccttgcac gcggtgttgc cgatatctat aagcaaatac
     1141 attttatcca tattatcttg ttggggcagc aaaggtaaga agattttgga atctaaataa
     1201 gttttgagag aaagaggatg gcttgactca cattcgggat gctctttacc ctgatataga
     1261 gtttctcctt cacctcattt accctgcagc ggtcaccaag tgattgaatg gcggtaagta
     1321 tcttcggaaa gagatctcct ctataatagg agtgcatgct gttggagatg aaataggcca
     1381 gcattacccc gttttttaaa attattttct caaatccgag attttgagcc acccttttga
     1441 gatgcactat gcggatcaat tcttcgagtt gcccgggtat cggaccaaag cgatcctcca
     1501 tctccctgcg caatgcctca atctgcctct catcgccgat atttgtgaga tccttgtaga
     1561 gacggatttt ttccgaagcc acttggacat attcgtcagg tatataggcc tccagatcgg
     1621 tctctatctg ggtatccacc attggagcgg attgcatggt atcgggcatg accatctcct
     1681 ctcctctgcc gatcctctct tcgcgaagct cgctcatcgc ctcgttgagg atgcgcatat
     1741 aggtctcaaa tcccatctcg gcaataaatc cgctctgctc cgctcccaat aggttgcccg
     1801 ccccgcggat atccaaatcc tgcatggcaa tattgaatcc gcttcccaaa tcagagaaag
     1861 cctcaatggc tcttatgcgt ctgcgggcat cctccgtaat ggatgcaata gggggtatca
     1921 gcagatagca gtaggccttg acattggagc ggcctaccct gcctctgagc tggtggaggt
     1981 cactaaggcc gaagcgctgc gcctgattga tgatcatagt gttggcgttg ggaatatcta
     2041 tgccgttttc tatgatggtg gtggccagca gtatgtcgtg ctctccggcg ataaactcca
     2101 caatcctttt ctccagcacc tcaggttcca tttgtccgtg tgccacacat atctgtgcct
     2161 caggtagcag tcgttgcaat atgtcgcgta ccgagagaat atcttctatc ttgttgtgta
     2221 caaaaaagac ctgccctccc ctctccaatt cgtagttgat gatatcggtt atcaactcat
     2281 ccttgaaatt tatcacctcc gtatagaccg gtttgcggtt gggcggaggg gtgttgataa
     2341 tggagaggtc cctcgctccg aggagcgaaa attggagcgt tctggggatg ggagtggcag
     2401 tgagggtgag agtgtcgaca ttgctcctca tctgacgaat tttctctttg gaggagactc
     2461 cgaacttttg ctcctcatcg atgatgagca atccgagatc cttgaagagt actttgctgt
     2521 ttaggatgcg atgagtgccg atcaggatat ccaccctgcc cgccctgagc tcttccagta
     2581 tgcgagatat ctcttttgta ttgcgcaatc gactaatata ttccaccctg caagggaaat
     2641 ctttcagacg cgaggagaag gtatgataat gctgaagcgc aagaatggtg gtcggcacca
     2701 aaactgccac ctgcttgctg tcgcaaacag ccttgaatgc tgctctgatg gccacttccg
     2761 tcttgccaaa tcccacatct ccgcagacca gacgatccat cggcctgtcg ctctccatat
     2821 cttcctttac ttttttggaa gcaagggcct ggtcgggggt atcttcatac ataaatgagg
     2881 attccagttc gtgcatgaga taattgtcgg ctgaaaatgc aaatcctttt gaatgttgtc
     2941 tggcggaata gagttttatc aggtcatcca ctatatcctt taccttggat ttggttctgg
     3001 acttgagatt ttcccacgcc ttggtgccga gtttatagat tttcggcggc tctccgtccc
     3061 tcgatttgta tttggagatg cggtgaatgc cgtggatgga gacaaatacc acgtcgttgt
     3121 ctttatagac caactttatt gcctcctgca ctttaccgtt gacatttgtc ttgacaagac
     3181 ctccgaactt acccactcca taatctatat gtaccacata atctcccatt tggaatgcca
     3241 ttatctcatt tatggtaagg cgttccgatc tctcaacttg tcgtttgacc tttatcttgt
     3301 gatagcgctc gaatatctga tggtcggtat agtagcagtt tttagaaatt ctatccacat
     3361 aaccctcatg aagggtaagc ggcagaaaat cggggcgtac cttttcgccg gtatctacca
     3421 atatctgcct tagacgatcg gtctgggcct catttgggct taggatagtc acgccatatc
     3481 cctggtccat cttgcttcgg atatcctgcg agagaatttc gaaattttta ttgaatgtgg
     3541 gctgaggaat cacatcattt tctcctgtgg ggttaacccc tctcagatcc cataaaacag
     3601 cattttcggg tagaatttcc cagaatggga ccagtgagct ctcatctatt cctttatgaa
     3661 tgtcgggaaa tatatccaca ctttccagac tctccttcga gagctgggta ttgatatcaa
     3721 attttctgat ggattctacc tgatctccga aaaaatctat ccttaccggc aggttgtctg
     3781 cgaatgaaaa tatatctatt ataccacccc tcaatgcaaa ctgtcccggc tccgacgtaa
     3841 aatccacctt ttcaaatccg ctttcgtaga gcacctcctt tataaattcg tgggagagtc
     3901 tgtcgccctt ttttattgaa agtatggtgc gtctgatttc gcttttctt
//
LOCUS       DAOSIE010000125         3947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_20206_length_3947_cov_3.942703, whole genome shotgun sequence.
ACCESSION   DAOSIE010000125 DAOSIE010000000
VERSION     DAOSIE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20206_length_3947_cov_3.942703"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2077
                     /locus_tag="PLI69_04570"
     CDS             <1..2077
                     /locus_tag="PLI69_04570"
                     /inference="COORDINATES: protein motif:HMM:NF009397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOG41796.1"
                     /translation="ILPDFQTGGLADCSKYNRGMRGGRIKVRAKIVKVDRRTLSITEL
                     PFGQTTDSLIDSIIKANERGKIKIRKVDDMSADNVDINIQLQNDISPDKTIDALYAFT
                     NCEVSVSPNACVIHDRKPHFMGVDEILRYNAMHTRDLFRQELQIQLEELENDWHFSSL
                     EKIFFEKKVFRILENDAATWEQQLADVEAGMKVYQHLLRREITGADIDKLVEKPVRKI
                     SRFDIKAAEERILGIENNIEEVKNHLEHLTEYTIMYFRQLKKKYGKDYPRRTELITFE
                     TIQAEKVVVSNAKLYANKAEGFIGIDQKKVEDSEYICDCSDIAEIIVFLKNGTYLVTK
                     VSDKAFVGKDIIHAAVFNRKDERTIYNAIYRDGKRGTYYAKRFSVTSITRDKEYDLTQ
                     GKEGSSVVWFTANPNGEAETVRIYYRPRPKLKKLSEDYDFSQLAIKGRSSRGNIVTKN
                     NTIHRINIKSKGVSTLGGKEIWFDTDVQRLNDAGRGLFLGEFFQDESILVICKDGTFY
                     TTSYDLSNRYQGDILTIGKFDEEKTYSVLYYDHAAGSPYIKRFSFELNANIPASFIAE
                     SKGSHLLEISEDPWPQMEITFKGKNANRKAELVDVEEFIGKKSYKAKGRKVSPYDVLQ
                     VRFVEPLDKSDAEEIEEQENGEDDENYNDRNDNIERNDKDMPTTPGKVISVPEPVIMD
                     EGPRELTLF"
     gene            2091..2456
                     /locus_tag="PLI69_04575"
     CDS             2091..2456
                     /locus_tag="PLI69_04575"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOG41797.1"
                     /translation="MSKKKILILVLCLCGAILFFVLDPESAIFIKCPFYSLTGYSCPG
                     CGAQRAIHDLLHLDIAGAFRHNALLVSSIPLIAVVMILERRKRSYPRLYTLFNSPLFL
                     FLLIALLFAWWILRNVFQI"
     gene            2534..2989
                     /locus_tag="PLI69_04580"
     CDS             2534..2989
                     /locus_tag="PLI69_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41798.1"
                     /translation="MRLISRLIVALFFSLATLSITSCEWFNETHTVVEIIGGENWMGS
                     KTVNLINGETTGLTKLVTFTEAGTQCRVMTGLYSLNVIDTELLKVKKENDDTILLTNM
                     DSGKTLYTCICNRDEDGKVISMTMTWTIHTDYESVISENPLTVILKKAN"
     gene            complement(2986..3489)
                     /locus_tag="PLI69_04585"
     CDS             complement(2986..3489)
                     /locus_tag="PLI69_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOG41799.1"
                     /translation="MAIIRELNGITPKIGKRCFIAENAVIVGDVVIGDDCSIWYGAVL
                     RGDVNPIIIGDRVNIQDGAVLHTTYKESIVEIGNDVSVGHNAIVHGAKVGNDVLVGMG
                     AILMDNAVIPDNTIIAAGAVVLTNQVLESGIYAGIPAKRIKEGSEAIKAAAHENAKGY
                     VTYKEWY"
     gene            complement(3503..>3947)
                     /locus_tag="PLI69_04590"
     CDS             complement(3503..>3947)
                     /locus_tag="PLI69_04590"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOG41800.1"
                     /translation="LFAILALVVLVVIAVSGDHFIKEHLWDHVIRKHFLSIFLWTTGA
                     LLLVQLGLHYLNIESWTSDNIPLMILIAVLVGLIPESGPHLVFVALYASGLVPFSVLL
                     ASSISQDGHSSLPLLAETKKGFLKAKLVNMIIGLIVGYGAYLLNF"
BASE COUNT         1184 a          915 c          859 g          989 t
ORIGIN      
        1 aatactcccc gatttccaga ccggaggtct ggctgattgc tcaaaatata accggggcat
       61 gagaggcggt aggatcaaag tccgcgcaaa gatagtcaag gtggacagga ggactctctc
      121 catcaccgaa ttgccattcg ggcagaccac cgattccctg atagactcca taatcaaagc
      181 caacgaaagg ggtaaaatca agatccgcaa ggtggacgat atgtccgcag acaatgtgga
      241 catcaatatt caactgcaga atgacatttc gcccgacaag actatagatg ccctatatgc
      301 cttcaccaac tgcgaagtct ccgtctcccc caatgcctgc gtaatccacg accgcaaacc
      361 ccactttatg ggcgtggacg agatacttag gtacaacgca atgcacaccc gcgacctctt
      421 ccggcaagaa ttgcagatcc agcttgaaga gctcgaaaac gattggcact tctcttctct
      481 ggaaaagata tttttcgaaa agaaggtatt cagaattctg gagaacgatg ctgccacctg
      541 ggagcagcaa ctcgccgatg tcgaggccgg aatgaaagtg tatcagcacc tccttcggag
      601 ggaaataacg ggcgctgaca ttgataagct tgttgagaaa cccgtaagga aaatctcccg
      661 tttcgacatc aaggctgccg aggagaggat tctagggatc gaaaataata tagaggaggt
      721 caagaaccac ctggaacact tgaccgagta cactataatg tacttcagac aactcaagaa
      781 gaaatacgga aaggattatc cgcgccgcac cgaactcatc acctttgaga caatccaggc
      841 tgaaaaagtg gttgtctcca acgcaaaact ctacgccaat aaagctgaag gctttatcgg
      901 aattgaccag aaaaaggtgg aggattccga gtatatctgt gactgctctg acattgcgga
      961 gatcatagtc ttcctcaaaa acggcaccta tctggtaacc aaagttagcg ataaggcatt
     1021 tgtaggcaag gatataatac atgctgcggt atttaaccgc aaggacgaga ggaccatata
     1081 caatgccatc taccgtgatg gcaagcgagg cacctattat gcgaagagat tttccgtcac
     1141 aagcattacc cgcgacaagg agtacgacct gactcagggc aaagagggat ctagcgtggt
     1201 gtggtttacc gcaaatccca atggtgaagc agagactgtc agaatctatt atcgtccgag
     1261 gcccaaactc aaaaaactct ccgaagatta tgacttctcg cagcttgcca tcaaaggtag
     1321 aagttccaga ggcaatattg taaccaaaaa taacactata caccgcatta acatcaaatc
     1381 caagggcgtt tctaccctcg gaggcaaaga aatatggttt gacaccgacg tacagcgcct
     1441 caacgatgca ggtcgcggtc tattcctcgg agaatttttc caggatgaaa gtattctggt
     1501 aatctgtaag gacggcacat tctataccac atcctacgac cttagcaata ggtaccaggg
     1561 agatatattg acaataggaa agtttgatga agagaaaacc tactcggtac tatactacga
     1621 ccacgctgca ggatctccct atatcaaacg attcagtttc gagctaaacg ccaatatccc
     1681 cgcctccttt attgcagaat ccaaaggctc ccatctgctg gaaatcagcg aagatccctg
     1741 gccgcagatg gaaatcacct ttaaggggaa gaatgccaat cgtaaagcgg agctggtgga
     1801 tgtcgaagaa tttataggaa agaaaagtta caaggcaaaa ggaaggaaag tctcccctta
     1861 cgacgtactt caggtacgtt ttgtcgaacc attggataaa tctgatgccg aagaaataga
     1921 agagcaggaa aatggagagg acgacgaaaa ctataacgac agaaacgaca atatagagag
     1981 gaacgacaaa gatatgccga ccacaccggg aaaggtaata tcggtacctg aaccggttat
     2041 tatggatgag ggtccacgcg aactcactct tttttaagag actttaaata atgtccaaaa
     2101 agaagattct gatactcgtt ttatgccttt gcggtgcgat attgtttttt gtacttgatc
     2161 cggagagcgc tatttttata aaatgcccct tttactccct gaccggctat tcctgtccgg
     2221 ggtgtggagc gcagagagcc atccacgatc ttctccattt agacatagcc ggagcatttc
     2281 gtcacaatgc attgctcgta tcatccatac cgctgatagc tgtagtgatg attcttgaac
     2341 gccgcaaaag atcatacccc aggctctaca ctcttttcaa ctctcctcta ttcctgtttc
     2401 tcttaatagc actgctcttc gcctggtgga ttctacgcaa tgttttccaa atctaggata
     2461 gatatttatt aatgttgtgc aacgaaatcg ccattttttg catctatatt gtgatgactt
     2521 tagaacaatt gttatgagac ttatatcccg ccttatcgtt gcattattct tctcacttgc
     2581 cactttaagc attacctcct gcgaatggtt taatgaaacc catacggtag tagagatcat
     2641 agggggagaa aactggatgg gatccaagac cgttaatctt atcaatggcg agaccaccgg
     2701 attgaccaaa ctggtgacat ttaccgaagc aggcacacag tgtagggtta tgacgggact
     2761 ttatagtctg aatgtcattg ataccgagtt gctcaaagtc aaaaaggaga atgatgatac
     2821 cattcttctg acaaatatgg atagtggcaa aacgctatat acctgtatct gcaatcgtga
     2881 cgaagatggc aaggtgataa gtatgacaat gacatggacg attcatacgg actatgaaag
     2941 tgtgattagc gagaaccctc tgacagttat tctcaaaaaa gccaattagt accactcttt
     3001 gtacgtgaca tagcctttgg cattttcgtg tgcagcggct tttatggctt cagaaccttc
     3061 cttaatgcgc tttgcaggaa taccggcata aatgccggat tccagtactt gattggtcaa
     3121 tactactgct ccggcggcaa tgatggtgtt gtcaggaatg acggcattat ccataaggat
     3181 tgctcccata cccactagga catcattacc tacctttgcc ccgtggacga tggcattgtg
     3241 tcctaccgag acgtcattac ctatttcgac tattgactct ttatatgttg tatggagtac
     3301 cgccccatcc tggatgttca ctctatctcc tattattatg ggattgacat ctcctcgtag
     3361 caccgctcca taccagatgc tacagtcatc tccgatgact acatccccaa ctatgaccgc
     3421 attctcggcg ataaaacatc tcttgcctat ttttggggtt atgccgttta attctcttat
     3481 gattgccata ttgtattcta ttttaaaaat tcaaaagata agccccgtac cctactatca
     3541 gtcctatgat catatttacc agttttgcct tcaagaagcc tttctttgtc tcggcaagga
     3601 gaggcaacga agagtgaccg tcctgggaaa ttgaacttgc cagcaatact gaaaaaggca
     3661 ccagaccact tgcataaagg gctacaaaga ccaaatgagg gccggattcc ggaattagtc
     3721 cgaccagtac cgcaatcagt atcatcaatg ggatattatc gcttgtccag gactctatgt
     3781 tcaaataatg aagacctagc tgtactaaaa gcagcgctcc ggttgtccat agaaatattg
     3841 ataaaaaatg cttgcgtatt acatgatccc acaaatgctc tttgataaaa tggtcacccg
     3901 aaaccgcaat taccacaagc acgactaaag caagtattgc gaaaagc
//
LOCUS       DAOSIE010000126         3915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_20401_length_3915_cov_3.051036, whole genome shotgun sequence.
ACCESSION   DAOSIE010000126 DAOSIE010000000
VERSION     DAOSIE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20401_length_3915_cov_3.051036"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLI69_04595"
     CDS             <1..513
                     /locus_tag="PLI69_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOG41801.1"
                     /translation="ITTLASTNGMSGMVNTVWIIICVIMLGGALSASGMIQTITESML
                     RFMHNLVSMVTSTIFTCIFCNIILADQYMAILLPGKMFSDVYRRKGYAPEMLSRTLQD
                     SATVSSVMVPWNTCAVAQAGVLGVACMTWFPYAFFCFITPIISIIIVAMGYKIRRLPV
                     ENGEPATAEA"
     gene            552..1079
                     /locus_tag="PLI69_04600"
     CDS             552..1079
                     /locus_tag="PLI69_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG41802.1"
                     /translation="MKIEDNKVVSLIYNLEVNGETKDSCDKENPLEFIMGMGYLLPKF
                     EENLLGKGVGDKFDFILSAAEGYGELNPQAIVDLPKNIFEVDGVLQEELLVPGNYIPM
                     MNQMGGVIPGKVLEVSEESVKMDFNHELAGQSLHFTGEITAVREATEEELTNGLHGER
                     AMHNCEGNCSSCGGC"
     gene            complement(1076..2113)
                     /gene="tsaD"
                     /locus_tag="PLI69_04605"
     CDS             complement(1076..2113)
                     /gene="tsaD"
                     /locus_tag="PLI69_04605"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOG41803.1"
                     /translation="MDDMVTILAIESSCDDTSAAVLREEYLLSNVMASQEVHKKYGGV
                     VPELASRAHQQNIIPVASQALEQAGVTVKELDAIAFTRGPGLLGSLLVGTAFAKGLAI
                     AIGKPLVEVNHLQGHILSHFIRIEGREHREPKFPYLSLLVSGGHTQIVKVNDYLDFEI
                     LGHTIDDAVGEAFDKCAKLLGLGYPGGPIIDRLAKEGNASAFRFAKPNIAGLDYSFSG
                     IKTSLLYFLRDSIAENPAFLEENKKDICASYQKDLIDILLKKLILASKQTGIKEIALG
                     GGVSANSYLRERVTFEGEKRGWNVFLPEFRFTTDNAAMIGIAGLYRYRKGLFAPIDVA
                     PISRAADYNQL"
     gene            2215..>3915
                     /locus_tag="PLI69_04610"
     CDS             2215..>3915
                     /locus_tag="PLI69_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41804.1"
                     /translation="MVILTALIPTTLYIAIQMPAFQTFVGNKVSSALSEKINGNISVG
                     KISYALFNRLIVKNVLITGPAGDTLLYANKISINLQTRSFISGEMKAGKVVIDGGEFN
                     IFKITPEESNLALLLKKNEAQHREELSEATEEEPDKRPEEWPKSVNIDKIEIRDFTFR
                     ALDTFDITTYSDPRTIDWRNIVLNDINLSAHNLHIDEDVAIELDNLSFKEGNGLNIKT
                     FSGNIRLGDVMEYNGLDTITRKEIYIDDMIYDDGYSKLLTEYYSMRFDDFSDFSDYVR
                     KVYMGLDLKEGSFLDFQSLRSYTDALSYFTLKFYLSGETKGTVSNLITRSLKVQSKTK
                     QTLLDFSAHLSGLPKASETMASFDIKRCYTTTAEIADIVESVTDTPIDKKAISSFAKG
                     ERIHFDGSLNGFFEDFVAFGELGSEDLGNFKVDIICRNERGAGYELIGFIKSRAFDLG
                     RLMDNSLLGDFTCDATVSAVLGKESSIDIENISISELGFNHYNYSNILATGRLANNEF
                     DGRVICSDPNLNFMFHGVFALAGKSSNSLYKFNLALGHADLHALNFDKREISRIKLLA
                     D"
BASE COUNT         1151 a          874 c          861 g         1029 t
ORIGIN      
        1 ataactactc tcgcctccac aaacggtatg agtgggatgg tcaacaccgt ctggattata
       61 atatgcgtga taatgcttgg aggcgccctc tctgccagcg gaatgattca gaccataacc
      121 gagagcatgc tgcgcttcat gcacaattta gtgagcatgg taacttccac aatttttacc
      181 tgcattttct gcaacatcat acttgcagac cagtatatgg ccatcttgct acctggcaag
      241 atgtttagtg acgtttacag gaggaagggc tacgcccctg agatgttgag ccgcacattg
      301 caggactccg ctaccgtgag ctccgtaatg gtaccctgga atacttgcgc ggttgcacaa
      361 gccggtgtac ttggagtcgc ttgcatgacc tggttcccct atgcattttt ctgctttata
      421 actccaataa tatcgattat catagtggct atgggataca agatacgccg tctccctgtt
      481 gagaatggcg aacctgcaac tgcagaagct taacgaattt aacaacaata acaactgaaa
      541 ttcactataa aatgaaaatt gaagacaaca aagtagtatc tctgatctat aatctggaag
      601 tcaatggtga gaccaaagac tcctgcgaca aggaaaaccc gcttgaattc ataatgggca
      661 tgggctatct gctccctaaa tttgaggaaa atctcctcgg caagggtgta ggcgacaaat
      721 ttgactttat cctttcagct gccgaaggtt atggcgaact caacccccaa gctatcgttg
      781 accttcccaa aaacatattc gaagtagatg gtgtactcca ggaagagctt ctcgtgccgg
      841 gcaattatat ccccatgatg aaccaaatgg gtggtgtaat ccccggtaaa gttctggaag
      901 tgagtgagga aagcgtaaaa atggacttca accacgaact tgcgggacaa tcactccatt
      961 tcacaggaga gattactgca gttagggaag ctaccgaaga ggagcttacc aacggacttc
     1021 acggtgagag agcgatgcat aactgcgaag gcaactgctc ctcttgcgga gggtgctaaa
     1081 gttgattata atctgcagca cgtgatatag gcgctacatc tattggcgca aaaagtcctt
     1141 ttctgtaacg atagagtccg gctatgccta tcattgctgc attgtcggta gtaaatctga
     1201 attcgggcag gaatacattc cagccccgtt tttctccttc aaaagtgaca cgctctctga
     1261 gatacgagtt ggcggaaact ccaccaccga gggcaatctc ctttattccg gtctgttttg
     1321 aggcaaggat gagtttcttg aggagaatat ctatcagatc tttctggtaa gaagcgcaga
     1381 tatccttctt gttctcttcg agaaaagcgg gattttcagc aatactatcc ctgagaaaat
     1441 agaggagcga tgtttttatg ccgctgaaac tgtaatccag accggctata ttgggctttg
     1501 caaaacgaaa tgcagaggca tttccctctt ttgcaagccg gtcgataatc ggaccgcccg
     1561 gatacccgag tccaagcaat tttgcacatt tatcaaatgc ctctccgacg gcatcgtcta
     1621 tcgtgtggcc gagtatttca aaatcaagat aatcattgac ttttacgatc tgtgtatgtc
     1681 ctcctgagac aagcagtgaa agatagggaa acttcggttc cctatgttcc ctaccctcaa
     1741 tcctgataaa atgagaaagt atatgacctt gaaggtggtt tacttcgacg agaggcttac
     1801 caatagcgat ggcaaggcct ttggcaaagg cagtacccac caaaagcgac cccaaaagtc
     1861 cggggccacg ggtaaatgct atggcatcca actcttttac ggtgacaccg gcctgttcca
     1921 gagcctgtga ggctaccgga ataatattct gctgatgcgc acgtgaagca agttccggta
     1981 ctacaccgcc atactttttg tggacctcct gagaggccat gacgttggat aacagatatt
     2041 cttccctcag cactgctgct gaagtatcat cacaggagga ttctatcgca aggattgtca
     2101 ccatatcgtc aatttggatt gaaatttgta cgcaaaatta gtaaaaatta gtacttttgt
     2161 ttgatatact gaaaattaaa gcacatcaag acctggaaaa aaatagtcct tgcggtggtc
     2221 attctcacgg cacttatccc gaccaccctc tatattgcaa tacaaatgcc tgcattccag
     2281 acatttgtag gcaataaggt ttcttctgcg ctttccgaaa aaataaatgg caacatatcc
     2341 gttggcaaaa tctcatatgc ccttttcaac cgtttgattg tcaagaatgt tttgattacc
     2401 ggccctgcag gcgacactct tctctatgcg aataaaattt ccatcaatct acagacgcga
     2461 agttttattt caggtgaaat gaaggcaggt aaggtcgtta tagatggagg tgaattcaac
     2521 atcttcaaaa taactcccga agagagcaat ctagctctac ttttgaaaaa aaatgaagca
     2581 cagcataggg aagaattatc ggaagcaacg gaagaggagc ctgacaagag accggaagag
     2641 tggcctaaat cggtcaatat tgacaaaata gagatacgag acttcacatt cagagcactt
     2701 gacacattcg acatcactac ttatagtgat cccaggacca ttgactggcg caatatagtc
     2761 ctaaatgata tcaatctctc cgcacataat ctccatatag atgaagatgt ggcaatagaa
     2821 ctggacaatc tctccttcaa agagggcaac ggactaaata taaagacctt tagcggaaat
     2881 atacgtctcg gcgacgtaat ggaatacaac ggacttgata caattacccg aaaggagatc
     2941 tatatcgacg acatgatcta tgatgacggg tattccaaac ttttgacaga gtattactca
     3001 atgagatttg acgatttcag tgacttttcg gattatgtga gaaaggttta tatgggcctt
     3061 gatctcaaag aggggtcatt tctggatttc caatccctga gaagttacac agatgctctt
     3121 tcatatttta ctttaaaatt ctatctgtcc ggtgaaacta aaggcactgt aagcaatttg
     3181 attacacgtt cgttgaaagt acaatccaaa acaaaacaaa ctttacttga tttctcagct
     3241 cacctctccg ggctgcctaa agccagcgag acaatggcca gttttgatat taagagatgc
     3301 tacaccacta ccgccgagat agccgatata gtggagtctg tgaccgacac ccctattgac
     3361 aaaaaagcaa tcagctcttt cgcaaaaggt gaaaggatcc attttgacgg aagcctgaac
     3421 ggcttcttcg aagactttgt ggcattcggt gaactcggat ccgaggatct gggaaatttc
     3481 aaggttgaca tcatatgccg caatgaaaga ggagccggat atgaacttat cggatttatt
     3541 aagagcaggg cattcgacct cgggcgtctt atggacaact cccttttggg tgattttacc
     3601 tgcgatgcca ctgtttcagc agtattaggg aaggaatcaa gcattgatat cgagaatata
     3661 agtatcagcg aactcggatt caaccattac aactacagca atatactggc aacgggaaga
     3721 ctcgccaata atgagtttga cggacgcgtc atatgcagcg accccaatct taactttatg
     3781 tttcatggtg tcttcgccct tgcaggcaag agcagtaatt ctctgtacaa atttaatctg
     3841 gcactgggtc atgcagacct tcacgcactc aatttcgaca agagggaaat ttccagaatc
     3901 aaactccttg ccgat
//
LOCUS       DAOSIE010000127         3914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_20407_length_3914_cov_3.959057, whole genome shotgun sequence.
ACCESSION   DAOSIE010000127 DAOSIE010000000
VERSION     DAOSIE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20407_length_3914_cov_3.959057"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLI69_04615"
     CDS             <1..459
                     /locus_tag="PLI69_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG41805.1"
                     /translation="ALSAGYGTYYHDAVDKKFRDQFKEHLTPFVGLTLKVPISSSIAT
                     VHRIRRAKSLLTGSNVALEKARRRLSDEIELAVAECRSCRSRCIAAEKSRKASEELLR
                     MNEFKLEVGEISATDYIISKNLCLKATSEHVQAYFQYIFQRMIVEHYLGK"
     gene            481..1677
                     /locus_tag="PLI69_04620"
     CDS             481..1677
                     /locus_tag="PLI69_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG41806.1"
                     /translation="MKIEKIKFKKWHIIALISIVVLIVIIVASGGCSVTEVRLFTIEK
                     MDLVESIPANGKVRPVIEVSITPDVSGEIVELNCREGDNVSKGDVLVKIRQDVYISMV
                     ERAEAVLNAARSDYLQQKAQFKQAEINYKRNNILYKENVISAAEYETSQLNYEIAREQ
                     LTAAQYGVSSGRAALKEATENLTKTVICAPMDGTVSMIAVKIGERVVGTSQMAGTEIM
                     RIADLSDMEVVVDVNENDILKLNPGNSAKIDIDACPGQLFEGVVTQIANSATNLYSNF
                     EQVTTFEVRIGIKSDTMAGVTFPEALPFRPGMSARVTIVTGQRADIVTVPIEAVFIRN
                     SMEYVWKVDEKNSVHATPVVSGIQDFSSIEIVSGLEPGNRIVAGPYSAVSKELEEGMK
                     VRGATY"
     gene            1694..2374
                     /gene="tsaB"
                     /locus_tag="PLI69_04625"
     CDS             1694..2374
                     /gene="tsaB"
                     /locus_tag="PLI69_04625"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOG41807.1"
                     /translation="MKETVLLLLETSTEVCSTVLTKGAKVIASRFCDLPKSHASKLAV
                     QISEVLAEGGITVSDCDAVAVSEGPGSYTGLRVGVSTAKGLCFGSGKPLIAISTMKIL
                     AMAAAGKADKIVALIDARRMEAYCAVFDSDGAPLSKVESLIFDKKSFKELLDEGSVIF
                     IGNAADKLQEIISHPNAIFEQCSPLAENMIIPATEAFVKKEFKDVAYFEPFYLKDFVA
                     GISKKQLL"
     gene            complement(2371..3489)
                     /locus_tag="PLI69_04630"
     CDS             complement(2371..3489)
                     /locus_tag="PLI69_04630"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG41808.1"
                     /translation="MKRFCSFAFALLLFAACSQRPTAVIEASIEGADEVQIVLQKLNF
                     SKIQVVDTITTAPDGSFTYKVKLSGTSPGFYYLYYEGTKLASMVLLPKDKVMVNADLE
                     GNFTVTGSEESLLLQAIDQEFLKTSAQLSELADKSLSGEDVGLDMGKTYIEYRKTALK
                     HIFGHPNSITAATTLFQRFNDNLPVFGEVSDFFIFKQVYDSLKVVYPQSEYVLALKDE
                     IAARERAFELSNKFSNAKEALFPDLEMPDNNGEMKRLSDFTGKVIILSFWSVAQDAHK
                     MFNNDLAELYQKYNSKGLEVYQVSLDIDKASWASIVRSQQLPWTSVNDGNGISSPAMT
                     AYNITQLPTFFIIGRDGTIAGKDLFEKDELEKVIKAQL"
     gene            complement(3493..3894)
                     /locus_tag="PLI69_04635"
     CDS             complement(3493..3894)
                     /locus_tag="PLI69_04635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOG41809.1"
                     /translation="MASIFTRIVNGEIPSYKVAEDDRFYAFLDISPLAEGHTLVIPKV
                     EVDYIFDLDEETYQGLFAFARKVAVAQKSVIPCKRIGMAVLGMEVPHTHIHLVPLHSE
                     GDLDFRKEKISLTPERFKEIAGLLSNAFNKI"
BASE COUNT         1128 a          847 c          949 g          990 t
ORIGIN      
        1 gcactctccg cggggtatgg tacatactac cacgatgcag ttgataagaa attccgcgat
       61 caattcaaag agcacctgac cccttttgta ggattaacac tcaaagtgcc tataagcagc
      121 tccattgcaa cagtccacag gatcagaaga gcaaagagtc tactcaccgg aagcaatgta
      181 gctttggaaa aggctcgcag gcgactctcc gatgagatag aactcgccgt agccgagtgc
      241 cgcagctgtc gcagccgttg cattgccgcg gaaaagagtc gcaaggcctc cgaagagctt
      301 ttacggatga atgaattcaa actcgaagtg ggagaaatct cagctaccga ttatatcatt
      361 tccaaaaacc tttgcctaaa agcaacgtca gagcatgttc aagcctactt ccagtatatt
      421 ttccagcgaa tgatcgtgga acactactta ggcaaataga gaaccattga aatagagaga
      481 atgaaaatag agaaaataaa atttaagaaa tggcatatca ttgcgctcat ttccatagtg
      541 gtactgattg tgataattgt agcttccggt ggatgcagcg tgacggaagt tcgtctgttc
      601 accatagaaa agatggattt ggtagaaagt attccggcca acggcaaagt gaggccggta
      661 atagaggtct ccataactcc ggatgtctcg ggagaaatcg tggagctcaa ttgccgtgaa
      721 ggggataatg tcagcaaagg ggacgtgctt gtaaagatcc gccaggatgt atatatttcc
      781 atggtcgaga gagccgaagc tgttctcaat gctgccagga gcgactacct tcagcagaaa
      841 gcccagttta agcaagctga aataaattat aagcgcaaca atattcttta caaggagaat
      901 gtaatatccg cagccgaata tgagacttcg caactgaatt atgaaatagc cagagaacag
      961 cttacggctg cacaatatgg tgtgagtagc ggcagagctg ccctgaaaga ggccacggaa
     1021 aatttaacaa aaacggtaat ctgcgcccct atggatggga cagtctcgat gattgcggtt
     1081 aaaatcggag aaagggttgt cggtacaagt cagatggcag gtactgaaat aatgcggata
     1141 gctgacttga gcgatatgga agtagtggta gacgtcaacg aaaatgatat tcttaaactc
     1201 aatcccggaa acagcgcaaa aatagatatt gatgcctgtc cgggacaact atttgaagga
     1261 gttgtgacac aaattgcaaa ttctgccaca aatttatact caaactttga acaagttact
     1321 acctttgagg tcaggatcgg catcaaaagt gatacaatgg caggagtaac tttccctgaa
     1381 gcactacctt tcagaccggg aatgtcggca agagttacaa tagtgacagg tcaaagggca
     1441 gatatagtga ccgtccccat cgaggctgtt ttcatccgca actctatgga gtatgtgtgg
     1501 aaagtggatg aaaaaaatag cgtacacgct acacctgtgg tgtcgggcat acaggacttc
     1561 agctccattg agattgtatc ggggctggaa cccggaaaca ggatagtagc cggaccctac
     1621 tcagccgtaa gcaaagagtt ggaagagggg atgaaagtgc gtggagcgac atattgaaga
     1681 ataataaatt gacatgaaag agaccgtatt gctactgctg gagaccagca ccgaggtttg
     1741 ctccacagtt ctgactaaag gagccaaagt gatagcctcc cgattttgcg acctgcccaa
     1801 gagccatgct tcaaaacttg cggtgcaaat cagtgaagtc ctggctgaag gcggtataac
     1861 tgtctcagat tgtgatgccg tagccgtgag cgaaggtccc ggctcttata cagggcttag
     1921 agtaggcgtg tctactgcca agggtctctg tttcggttct ggcaaacctc tgattgccat
     1981 ttccactatg aagattctgg caatggccgc agcaggcaag gctgataaga ttgtagccct
     2041 tatcgatgcc cgaagaatgg aggcctattg tgctgttttt gacagtgacg gagcccctct
     2101 gagtaaagtg gagtccctta tttttgacaa gaagagcttt aaagagctgc tggatgaagg
     2161 cagcgtaatc tttatcggga atgcggctga caagttgcag gaaataatca gccatccaaa
     2221 tgctattttt gaacagtgta gtcctttggc ggagaatatg attattcccg ctacagaggc
     2281 attcgtaaaa aaagagttca aagatgtcgc ctactttgaa cccttctatt taaaagattt
     2341 cgttgccggt atcagcaaga agcagctgct ctaaagctgc gctttgatca ctttttccaa
     2401 ttcatccttt tcaaacaggt ctttgccggc gattgtgcca tcccttccta ttatgaaaaa
     2461 tgttggtaac tgggttatat tgtaggctgt catcgcgggt gaactgatgc cgttaccatc
     2521 atttactgag gtccatggta gttgctgaga ccttacgata gaggcccagg aggccttgtc
     2581 aatatctaga gaaacctggt agacctccag ccccttggaa ttgtattttt gatagagttc
     2641 tgcaagatca ttgttgaaca ttttgtgagc atcctgcgct acgctccaga atgatagaat
     2701 aatcactttg ccggtaaaat cagaaagacg cttcatctct ccattgttat cgggcatttc
     2761 cagatcggga aagagcgctt ctttagcatt ggaaaattta ttgctcaact cgaaagccct
     2821 ttctctggcc gcaatctcat ctttgagtgc aagaacatac tcggattgtg ggtatactac
     2881 ctttagcgag tcataaacct gcttaaatat gaaaaaatca ctcacctcac caaataccgg
     2941 gagattatcg ttgaaacgct gaaacagcgt tgtggctgcc gtaatggagt tggggtggcc
     3001 gaaaatatgc ttcaaggcag ttttacgata ctctatatat gtctttccca tatccaggcc
     3061 gacatcttcg cctgagagac tcttgtcagc caactcggag agctgcgcag aggtcttgag
     3121 gaactcctgg tcaatggcct gcaaaagtag tgattcttcg ctacctgtga ctgtaaaatt
     3181 gccttccagg tcagcgttaa ccataacctt atctttgggt aggaggacca tggaggctaa
     3241 cttggtgcct tcgtaataga gatagtagaa acccggcgag gtaccggaaa gcttcacctt
     3301 ataagtaaag ctgccatcag gtgcagtggt gatggtgtca acaacctgta ttttgctaaa
     3361 attaagcttc tgcagcacaa tctgcacctc gtcagcgccc tcaatagagg cctctatcac
     3421 tgccgttggc cgctgagaac aggctgcgaa gaggaggagt gcaaaggcaa atgaacaaaa
     3481 tctcttcatt atctatatct tgttgaatgc atttgaaagc aagccggcga tctctttaaa
     3541 acgttccggt gtaagggaga ttttttcctt acggaaatcg aggtcaccct cactatggag
     3601 tggcacaagg tggatatgcg tatggggaac ctccattccg agcacggcca taccgatcct
     3661 tttgcaggga attacactct tttgtgcaac agccactttc cgagcgaatg caaaaagccc
     3721 ctgataagtc tcctcatcca gatcaaaaat gtagtcaacc tcaactttgg gaataacgag
     3781 agtgtgaccc tctgccagag ggcttatgtc cagaaatgcg tagaacctgt cgtcctccgc
     3841 cactttgtaa gatggtatct caccatttac aatccttgta aaaattgatg ccatatctgt
     3901 gactatattg aaat
//
LOCUS       DAOSIE010000128         3902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_20489_length_3902_cov_3.865610, whole genome shotgun sequence.
ACCESSION   DAOSIE010000128 DAOSIE010000000
VERSION     DAOSIE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20489_length_3902_cov_3.865610"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..817)
                     /locus_tag="PLI69_04640"
     CDS             complement(209..817)
                     /locus_tag="PLI69_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HOG41810.1"
                     /translation="MDKNITQTTDIEKIRRAIVRGKYGSVYFASSFPNFNADYVSKLL
                     ATFEKEGLLERISKGVYLKARKTRFGISYPPIEEIVAEIAKRDKAKIIPTGETAANLL
                     GFSDQVPLQTCFLTTGTYRTLRIGKRTVLLKNAAPKNFEFKNEIVGVLVQALRSIGKD
                     NVTEEMKQKIPVILAKVPKNKYLDTDLRLAPVWIKKTIYSTL"
     gene            complement(978..1475)
                     /locus_tag="PLI69_04645"
     CDS             complement(978..1475)
                     /locus_tag="PLI69_04645"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOG41811.1"
                     /translation="MKEILIYKSKNSLVTNMMVTVLEANGIAYRQHDESIDQRPDSYG
                     PPPGISIYVFQKDYDRALKLINPIIKDNIEAPKPLCPKCGSEEVVAIPRHKYTKLVIL
                     LAVLLFLIPGLYFVYSRNLNISSQSLDITAIVMILSSIALMIVSKYQNVNHQCKECGR
                     KFNHI"
     gene            complement(1566..2336)
                     /locus_tag="PLI69_04650"
     CDS             complement(1566..2336)
                     /locus_tag="PLI69_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41812.1"
                     /translation="MKKIFLSLIAVIAFINLANAQDFVIMRNGDEIRAKVLEITVDEV
                     KYRLYDEPDGVIYTVWKTDIIMIHYESGRSEVFSKQVMPGPYYSQRVPVDGLRPGMKY
                     NELKHFYNYKDYIPSPSDFNPGWCGVASAFIPGLGECILGEWGRGLGKFFGNMALMSV
                     AVVSYNAGYYAGYHVGYHSEEAGWSLGVAALCSAAAVGINIWSIVDAVRIAKVKNMYE
                     QDLRQSYSFNLDLHPSVNYIQMGNKVEPTVGFTLALRF"
     gene            complement(2387..3139)
                     /locus_tag="PLI69_04655"
     CDS             complement(2387..3139)
                     /locus_tag="PLI69_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41813.1"
                     /translation="MKKIIYSLIVIFTSMSMANAQDLVTKRNGGDIKAKVLEITGEEV
                     KYRLYDEPDGVIYTVNKSDIVMIRYESGRNEIFDENAISDSYYSEKEPVGNLRPDMKY
                     NELKKMYNHKEYVPSSGDRYKPGWTGVASFLIPGLGGSINGEWKRGVGKFCGSVVLLT
                     AGSIYERKSHGDDAKGWHLAVAAACYVGVVGLDVWSIVDAVRIAKVKNMYEQDLRRTD
                     YSFNLDLHPSVNFVRMGNTIHPTVGFTLALQF"
     gene            3444..>3902
                     /locus_tag="PLI69_04660"
     CDS             3444..>3902
                     /locus_tag="PLI69_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOG41814.1"
                     /translation="MKKITDDVIYIGVDDLDIDLFESQYKVPQGISYNSYLILDEKVA
                     ILDTADARKGEEWEENLSKALGERQPDFLVVHHLEPDHSSMIAKVLEKYPKIQVVTGV
                     RSVKMLSQFFDTPDIATRCIEMKEGDLLDLGKHKLRFYAAPMVHWPEVMVS"
BASE COUNT         1108 a          825 c          755 g         1214 t
ORIGIN      
        1 ttacttacaa tgaatcatct agactagaaa ttcatgaaca actaaggaaa cttactagaa
       61 ctgatctaat gagtctgaag agtaattgct attttgaaat aatccgtaca tcgctttgtg
      121 tatatgacga taaagacaaa tttgagaagt ggatttctcg tcaacattgt aattaatcca
      181 tctcgccatc tctctcctct taaaatgttt ataatgtgct gtatattgtc tttttaatcc
      241 aaaccggggc tagtctgaga tcagtgtcaa gatatttgtt tttgggtact tttgcaagta
      301 ttacgggaat tttttgtttc atctcctctg tcacattgtc tttaccaatg ctccttaggg
      361 cttgcaccaa tactccaaca atctcattct tgaattcaaa attcttaggt gctgcatttt
      421 ttaggagaac agtacgtttc ccaatcctta gagtcctgta tgtgccggtt gttaaaaagc
      481 aggtttgcaa tgggacctgg tctgagaatc caagaagatt ggcggctgtt tcgcctgtag
      541 gaattatctt tgctttgtct cttttagcaa tttcagcaac gatttcttct attggtggat
      601 acgatatccc aaatcttgtc ttgcgcgctt ttaaatagac gccctttgat atacgctcaa
      661 gaagaccttc tttttcaaaa gtcgcaagta attttgatac ataatcagcg ttaaaatttg
      721 ggaaggaaga ggcgaagtac actgagccat attttccgcg cactatagca cgtctaatct
      781 tctctatatc tgtcgtttgt gtgatatttt tatccataat taagtcgaaa taatccaaaa
      841 tcttttcgac tgctaatata gtgaatattt taatattatc aatcagatta atcccattgt
      901 tctaatcctc tttacccctc cttttaatgg ttcccttggg aaccaaagca gcgcagaagt
      961 gaagcaacac cgcttcgcta tatatgatta aacttcctac cgcactcctt gcactggtga
     1021 ttgacgtttt ggtatttgct gacaatcatc aaggcgatgc tggataggat catcactatt
     1081 gcggtgatat cgagagactg agaactaatg tttaagttcc tggaatagac aaaatagagt
     1141 cctgggatta ggaataaaag gacagcaagc agtatgacaa gtttggtata cttgtgccgg
     1201 ggaatggcta cgacctcttc acttccacat ttgggacata gtggtttcgg agcctctata
     1261 ttgtccttaa taatgggatt gattaacttc agtgccctgt cataatcttt ttggaaaaca
     1321 tatatggaaa tacccggagg cgggccatac gaatccggcc gttgatctat tgactcatca
     1381 tgttgtcggt aagcaatgcc attggcttca agtacggtaa ccatcatgtt ggtgacaagg
     1441 ctgtttttgc ttttatatat caatatctct ttcatagctc gcaaatttta attctcgtac
     1501 ttgacgattc cgacttcaca taatgataaa cctacatcag gtggcaggag ttaccaaaaa
     1561 aagttttaga accttaatgc aagagtaaat cctacggtgg gctctacttt gttgcccatt
     1621 tgtatgtagt ttaccgaggg atgcaggtca aggttaaacg agtaagattg tctcaaatcc
     1681 tgttcataca tattctttac cttggctatc cttacggcat caacaatgct ccaaatattg
     1741 attcctacgg cagctgcaga gcaaagcgct gcaacgccta gcgaccatcc tgcctcttca
     1801 ctatggtaac ctacgtgata ccccgcgtaa taccccgcat tataagagac tacggcaacg
     1861 ctcatcagtg ccatattacc aaagaatttg cccagacctc gtccccattc tccaaggata
     1921 cattctccaa gtccggggat gaatgcagat gctacaccac accagcctgg attaaaatca
     1981 ctcggggatg gaatgtaatc tttgtagtta taaaagtgtt taagctcatt atacttcatt
     2041 cccggtctga gaccatcaac aggtactctt tgggaataat aggggccggg catcacttgt
     2101 tttgagaata cctcacttct gcctgattca taatgaatca ttataatatc ggttttccat
     2161 acggtataga taacaccatc gggctcgtcg tataacctgt acttgacttc atccacagtg
     2221 atttcaagca ctttagccct aatttcatca ccatttctca tgattacgaa gtcctgggcg
     2281 tttgccaaat tgataaatgc gattacggcg atcagggata aaaatatttt tttcataata
     2341 ggattaattt tcaattaata attaaaccac taagcagtag tcctttttaa aactgtagtg
     2401 caagggtaaa tcctacggta ggatgaattg tgttgcccat tcggacaaag tttaccgaag
     2461 gatgcaggtc caggttaaat gagtaatcag tccgtctcag atcctgctcg tacatatttt
     2521 ttactttagc tatccttacg gcatcgacaa tactccaaac gtccagtcct actacaccta
     2581 catagcaagc agctgcaact gcaaggtgcc atcctttagc atcatcacca tgactttttc
     2641 tttcgtatat gctgccggca gttaaaagaa caacgctacc gcagaatttg cctacaccac
     2701 gcttccattc tccatttatg gaccctccaa gacccgggat gagaaatgat gctactcccg
     2761 tccatcccgg tttgtatcgg tcgcccgatg atgggacata ctctttatgg ttatacattt
     2821 ttttaagttc attatacttc atgtcgggtc tgagattgcc aacaggctct ttttctgaat
     2881 aataagaatc agaaatcgca ttctcgtcaa agatttcatt tctaccggac tcgtagcgga
     2941 tcataacgat atcggactta tttacggtat aaatgacgcc atccggctca tcatagagtc
     3001 tgtatttaac ctcctcgccg gtgatttcca ggactttggc cttaatgtcc ccgccatttc
     3061 ttttggttac gaggtcttga gcgtttgcca tactcataga tgtgaatatg acaatcagag
     3121 aataaattat ttttttcatg atttgaaaat aaatgctaca aaaggttttt taataaaaaa
     3181 tcgtccctta attgattgcg gttaaaactt ataatgctaa actacaatta ttttatgcaa
     3241 atatagtaga aaaaattagt gacaggttat tttattgtat cgttattact tagttctcac
     3301 aacagtagca ttaaaagttt cgagtcgcac ccggattaca ctcccgaaag gccaatttga
     3361 gtcatactcc tttttcaatt tggagggatt tactatcttt gcagcgcaaa agcgcaattt
     3421 ttatattaaa tacaaatgca attatgaaga aaatcactga cgacgttatc tacataggtg
     3481 ttgacgacct tgatattgac ctttttgaga gccagtacaa agtgccgcaa ggaatttcct
     3541 ataattccta tctcatcctg gatgagaaag tagccatttt ggataccgcc gatgccagaa
     3601 aaggagagga atgggaggaa aatctgagca aggccctggg agagaggcag ccggactttc
     3661 tggtcgttca tcaccttgag ccggaccatt cttcaatgat tgcaaaagta ctcgaaaagt
     3721 atcctaaaat tcaggttgta acaggtgtgc gatcagtcaa gatgctgtcg cagttctttg
     3781 atacgccgga tatagccacc cgctgcatag agatgaagga gggtgatctc ctcgacctgg
     3841 gaaaacacaa actccgcttt tatgcggcgc ctatggtaca ctggcctgaa gtgatggtat
     3901 cg
//
LOCUS       DAOSIE010000129         3900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_20499_length_3900_cov_4.006762, whole genome shotgun sequence.
ACCESSION   DAOSIE010000129 DAOSIE010000000
VERSION     DAOSIE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20499_length_3900_cov_4.006762"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLI69_04665"
     CDS             complement(<1..570)
                     /locus_tag="PLI69_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOG41815.1"
                     /translation="MKRLLTFAFFIAIAFPVSGESYDLLRRNLKSHIQYLSSDVLQGR
                     KTGTKGEKMAAEYLYDALESNGVIMLTDRNGQDFTIMSNGDSIHSRNIVGIIEGCDSL
                     LRDEYIVVGAHFDHLGTNNLMVDGTPVTQIFAGADDNASGVATLIELSRLVSENRFDF
                     PRSIIFVGFGAKEHGMAGSWYFVNRAFQGI"
     gene            complement(621..1727)
                     /gene="gcvT"
                     /locus_tag="PLI69_04670"
     CDS             complement(621..1727)
                     /gene="gcvT"
                     /locus_tag="PLI69_04670"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOG41816.1"
                     /translation="MKNTVFTEKHIALGAKMVPFAGYNMPIQYVGINEEHTTVREGVG
                     VFDVSHMGDIWVKGPNALAFLQYVTSNDVSTLVAGKAQYSCFPNGKGGIVDDVIIYCI
                     DSQTYLLVVNASNIEKDWAHLCSFADKFSMTPGKELYNASDEICQLAIQGPKAMEVMQ
                     KICDQDIMSMEYYTFKKLTVAGIPNAILSITGYTGSGGCEVYVANEDGIKLWDAIFAA
                     GEEFGIKPIGLGARDTLRLEMGFCLYGNDIDDTTSPLEAGLGWITKFNDVKGDFVDKE
                     YLLAQKNEGVKSRLIGFELIDKGIARHGALICDAEGTQIGVVTSGTMGPSVKKAIGLG
                     YVNVPFNKVDTEIYIDVRGKLLKAVVVKTPFYKK"
     assembly_gap    1804..1813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1814..2241)
                     /locus_tag="PLI69_04675"
     CDS             complement(<1814..2241)
                     /locus_tag="PLI69_04675"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG41817.1"
                     /translation="MKPIKIVTILLTASLLFSGCDFFRSILGKPTSKEIEAARIAQEA
                     AAAAKRDSIARAEEMARLAAEAARPVYSNYYVIMGCYKIPANAERFKARLKAKGYDVT
                     EMRFKNGYDVLAIYGSETLREAYRHRDALLERGEDPYDMW"
     gene            2365..3702
                     /locus_tag="PLI69_04680"
     CDS             2365..3702
                     /locus_tag="PLI69_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOG41818.1"
                     /translation="MSKFRQRFKEIVNISKDVDWSIASESIRNSIHFKGPNVWILAAA
                     IIVASIGLNVNSIPVIIGAMLISPLMGPIMGFGLGLGTNNTVLLRDSLKNLGIMVLIS
                     IIASTLYFIVSPLKMDNPTELLARTNPTIYDVMIALFGGFAGILETSRKEKGTVISGV
                     AIATALMPPLCTIGYGIATLKLNYALGALYLFFINGVFIALATFLMVKYLKFPLIKLA
                     DSAKQKKVSGTIFAFTLVLIIPSVISAVMVIHENRFNHNARKFISENRNVNKSYIYDH
                     EIKHEKMSGTLTLYMAGEALVSSDRELLYRSAEMYGIGRDQLTIIENAVIREELSQSD
                     LMKSFFERSDKEIKRREELLLQLQEELRTIRDKELPVAQIAKELLTQYPDLTGFSMSR
                     GSEVTLNDLQESERIFVLVKWRHPVDEEVITKLQKWLSVRLETENITVLQDKR"
     gene            3705..>3900
                     /locus_tag="PLI69_04685"
     CDS             3705..>3900
                     /locus_tag="PLI69_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41819.1"
                     /translation="MVRFTIFTVVILVLLSLVAPSRGVVFQEEEVSALSVTPFEDDTV
                     RILFIGDVMAHKLQLETALID"
BASE COUNT         1063 a          934 c          836 g         1057 t
ORIGIN      
        1 tatgccctga aaggctctgt tgacaaagta ccacgagccc gccatcccat gctctttggc
       61 gccaaaacca acaaaaataa tggaacgggg aaagtcaaat ctattttccg acaccagtct
      121 ggagagctcg atcagtgtcg caactccgga tgcgttgtcg tccgcacctg caaaaatttg
      181 tgtgacgggg gtaccgtcaa ccatgaggtt atttgtgccc aaatggtcaa aatgagctcc
      241 taccacgatg tactcatctc ttagaagaga atcacacccc tcgataatgc cgacaatgtt
      301 ccgagaatgg atgctatcgc cgttactcat tattgtaaaa tcctgtccgt tacgatcggt
      361 cagcattatg acgccatttg actcaagagc atcgtaaaga tattcagcag ccattttttc
      421 tcctttcgtg ccggtttttc tgccctgcag tacatcagat gacaaatatt ggatatgact
      481 cttgaggttg cgcctcaaca ggtcataaga ctctccggat acaggaaatg caatggcaat
      541 aaaaaaagcg aaagtgagca atcgtttcat aacagagata ttaacgaacc cactttcgca
      601 aaatgtatgc cctcccgaga tcacttttta tagaagggag tctttactac tacagctttg
      661 agaagtttgc cgcgtacatc tatatagatc tcggtatcaa ctttattgaa agggacattg
      721 acatagccga ggcctattgc cttcttcacg gaaggcccca tagtgcccga agtcaccact
      781 ccgatctgag tcccttccgc atcacagatt agtgccccat gacgtgcaat gcctttatct
      841 atgagttcaa atccgataag tctgcttttt acaccttcat ttttctgcgc cagcagatac
      901 tccttatcca cgaagtcgcc tttgacgtca ttgaatttgg ttatccatcc aagaccggct
      961 tcaagaggag aagtggtgtc gtcaatgtca ttgccataga gacagaatcc catttcaaga
     1021 cggagcgtat cacgagctcc aaggcctata ggcttgattc cgaattcctc acctgcggca
     1081 aaaatagcat cccacagctt gattccatcc tcatttgcta cataaacctc acatccgcct
     1141 gagccggtat aaccggtaat ggatagaatg gcattgggaa ttccggccac agtaagtttt
     1201 ttaaaggtat aatactccat ggacattatg tcctgatcgc atattttctg cattacttcc
     1261 atagccttgg ggccctgaat ggccaactgg cagatctcat ccgaagcgtt gtatagctct
     1321 ttcccgggag tcattgagaa tttgtcggca aagctgcaaa gatgagccca gtctttctca
     1381 atattagagg cattgactac caagagataa gtctgactat caatgcaata aataatcaca
     1441 tcatccacga tgcccccttt accgttagga aagcaggagt actgcgcctt accggcaaca
     1501 agggtagata cgtcgttgga ggtcacatac tgtaggaaag ccaacgcatt aggacctttt
     1561 acccagatgt cacccatgtg ggaaacatca aatacgccga caccttctct tacggttgta
     1621 tgttcttcat ttatgcccac gtattgtatc ggcatgttgt agccggcaaa agggaccatc
     1681 ttagcaccga gtgctatgtg cttttctgta aatactgtat ttttcattta ttgtttttat
     1741 gttgttttac ataataatat aatacttagt gctacttttc gtgtaaggcc atggacgtct
     1801 ggtnnnnnnn nnnatccaca tatcgtaagg atcttctccc ctctcaagca gagcatccct
     1861 atgcctgtaa gcttcacgta acgtctccga gccgtaaata gccagcacat cgtaaccatt
     1921 cttgaaacgc atctccgtca catcatatcc tttggcctta agcctggcct taaaacgttc
     1981 tgcatttgcg ggaattttat aacatcccat aataacatag taattggagt aaaccggacg
     2041 ggctgcctcc gctgcgagac gagccatttc ttctgccctc gctattgagt cccttttggc
     2101 agccgcagcc gcctcttgcg ctatcctggc tgcctcaatc tctttggacg taggcttgcc
     2161 caatatagaa cggaaaaagt cgcatccgct gaaaagcagt gatgctgtaa ggagaattgt
     2221 cacaatcttg atcggtttca tggctaacgg aattatgtgg gttaagtatc tctgagtatg
     2281 ttattgatat catacaaaaa taatcattta tagatgaaac gcaaaaattt tcctatcttt
     2341 acctctcaag aagatcattg ttttatgagc aaattcagac aacgtttcaa agagatcgtt
     2401 aatatttcga aggatgtaga ctggtctatc gcttcggaaa gcatccgcaa cagcattcat
     2461 tttaagggac ccaatgtgtg gattttggct gctgccataa ttgtcgcctc catcggactc
     2521 aatgtcaact ccatccccgt aatcataggt gcgatgctca tttcccctct gatggggcct
     2581 atcatgggtt tcggattggg tctgggcacc aataacaccg tgctactcag ggactcactg
     2641 aaaaatctcg gcattatggt gctaatcagc atcatcgctt ccacgctcta cttcattgta
     2701 agtccattga aaatggataa cccgaccgag ttgcttgcca ggaccaaccc tacaatttac
     2761 gacgtaatga tagcgctttt cggcggattt gcaggaattc tggagacttc ccgtaaggag
     2821 aaggggactg taatatcggg agtagccatt gctaccgccc ttatgcctcc tctctgcacc
     2881 ataggctacg gtatagccac actaaagctc aactacgcat tgggggccct atatctcttc
     2941 ttcattaacg gagtattcat cgcccttgcg accttcctga tggttaaata cctcaaattt
     3001 ccgcttataa aattagccga cagtgcaaaa cagaaaaagg tatccggtac catattcgca
     3061 tttacactag tgctgattat ccccagcgtt atctccgctg ttatggtcat ccatgagaat
     3121 cgcttcaacc acaatgcgcg taaattcatt tcagagaaca ggaacgtcaa taagagctat
     3181 atctatgatc acgagatcaa acatgagaaa atgagcggca ctctgactct ctatatggcc
     3241 ggtgaagccc ttgtctcatc cgatagggag ctgctctacc gctccgcgga aatgtacggt
     3301 atcggacgtg accaattgac aatcattgaa aatgcggtaa ttcgagaaga actctcccaa
     3361 tcggatctga tgaagagctt cttcgagagg agcgacaaag agattaagcg acgtgaggag
     3421 ctgcttctac agttacagga ggaactcagg actatacgag acaaagagct gccggtagcg
     3481 caaattgcaa aagagttgct cacccaatat ccggatctga caggattcag catgtccaga
     3541 ggcagcgaag taacactaaa tgatctccaa gagagcgaac gcatattcgt attagtaaag
     3601 tggaggcatc cggttgatga agaggtgatc accaaactcc agaaatggct ctctgtgcgt
     3661 ctggagacag aaaacattac agttttacag gataagcggt gagggtggta agatttacaa
     3721 tttttacggt agtaatactg gtactgctat ctctggttgc cccttctcgt ggagtcgttt
     3781 ttcaggagga agaggtttca gctctttcgg tgacaccgtt cgaggatgat accgtcagaa
     3841 ttcttttcat cggagatgtg atggcccata aactacagtt ggagaccgca cttatcgacg
//
LOCUS       DAOSIE010000130         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_20755_length_3863_cov_3.266282, whole genome shotgun sequence.
ACCESSION   DAOSIE010000130 DAOSIE010000000
VERSION     DAOSIE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20755_length_3863_cov_3.266282"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLI69_04690"
     CDS             <1..955
                     /locus_tag="PLI69_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOG41820.1"
                     /translation="GIDKEIVEIGRKINEKSRSIQTLQREIDTLESYYARLVRNTYKN
                     RDTKVWFMYLFASESIGQGYRRFSYLKNLSGVVNAQGVKIKEKQAQLEMEKAQLQNMK
                     KEAEATRAERELEYKQLLNEENISRQVADNLAKSRKQQMRELERKRQQVEKLNREIES
                     ILSKAVKTQEKEKTPIDYTLSGMFEQNKGKLPWPVAKGVVIGKFGVHYHPVYKNLKLP
                     ENNGIDISTSKNAEVLCVFDGVVKQIIMMPGYNQCILIQHGTYFTFYCKLKKVNVKTG
                     DKVATGEPLGILNEEGNTSEIHFQIWKGTTKQNPAVWLRPR"
     gene            complement(963..3782)
                     /gene="uvrA"
                     /locus_tag="PLI69_04695"
     CDS             complement(963..3782)
                     /gene="uvrA"
                     /locus_tag="PLI69_04695"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG41821.1"
                     /translation="MGGGTDHIEITGARVHNLKNIDLSIPRDRLVVVTGLSGSGKSSL
                     AFDTIYAEGQRRYMETLSAYARQFIGILERPDVEDIKGLSPIIAIEQKTTGRNPRSTV
                     GTITEIYDFFRLLYARAADAFSPESGKKMVRYTDEQITDLIIETHKGEKILLLAPLVK
                     GRKGHYKELFEQLIRKGYSQVRIDGEIRWLSDVKPLDRYKVHFVELVVDKLIPREEER
                     KRIRESVLTALHQGKGSLAIVRLEDDSIEHLSRNLICPESGLSFATPAPHTFSFNSPQ
                     GACPHCKGLGTIATVDISKIIPDPSKSIASGGIESVGMLRDNTTFRYLEAIALKYGFS
                     LHDPISTISEDALNLILYGSDELLRIGKGSDMEMASFQGVIALIYHNDEESDKVWDKK
                     ELFVDEMVCPVCNGTRLKSESLCFRIDDKNIAQVAGMEIDTLYEWVSGLHLKLSPRQR
                     LIAEDILKELKERIGFLKEVGLDYLSLDRTTRSLSGGESQRIRLATQIGSKLVNVLYI
                     LDEPSIGLHQRDNLKLINSLKALRDEGNSVMVVEHDQQMMENADWLIDLGPGAGEKGG
                     ELLYNGPPEDIASCNCQSPTIDYLLGHKSIELPSVRLKGNGKYITLEGATGNNLKNVT
                     LNLSLGLFVGISGVSGSGKSSLINETLMPILSNHFYRSKYKVLPYKSIRGIENIDKII
                     EIDQAPIGRSPRSNPATYTNVFTDIRKLFGETPDAKIRGFKANRFSFNVKGGRCEVCK
                     GAGVQVIEMNFLPPAYVTCKECNGLRYKPDTLAVKYKGKSINDLLNMTISQACLFFEA
                     IPSILQRLRTLEDVGLGYLKLGQQSTTLSGGESQRLKLSAELARRDTGNTLYILDEPT
                     TGLHFEDIKVLLRVLRRLVDQGNTVVIIEHNLDVLKSVDYLIDLGPEGGKGGGYIIAE
                     GTPEQVALRENSYTGGYLRGILGVK"
BASE COUNT         1068 a          935 c          871 g          989 t
ORIGIN      
        1 gggtatagac aaggaaatcg tagagatagg tcgcaaaata aatgaaaaga gccgctccat
       61 acaaactctc caacgcgaaa tagatactct cgaaagctat tacgcacgtc ttgtacgaaa
      121 tacctacaaa aaccgcgaca caaaggtctg gtttatgtat ctatttgcca gcgaaagcat
      181 aggacagggg tatcgaaggt tttcatacct gaaaaacctc tccggagtgg tcaatgcaca
      241 gggagttaaa atcaaagaga aacaggcaca acttgaaatg gagaaagccc aactccagaa
      301 catgaaaaag gaagctgagg ccacccgtgc agaacgtgag ctggagtaca aacaactact
      361 caatgaggag aacatcagtc ggcaggtggc ggacaacctt gccaaaagcc gcaaacaaca
      421 aatgcgcgag ctggagagaa aacgccagca agttgaaaaa ctcaaccgcg aaatagagag
      481 cattctcagc aaggccgtca aaactcagga aaaggagaaa acccccattg attacaccct
      541 ttcaggtatg tttgagcaaa acaaaggcaa actgccctgg cctgtggcca aaggggttgt
      601 gataggaaaa ttcggagtcc attaccatcc ggtctataag aatctcaaac tgccggaaaa
      661 taacggtata gacatatcca cttcaaaaaa tgccgaagta ctgtgtgttt ttgacggagt
      721 tgtcaagcag atcataatga tgccgggcta caatcaatgc atccttatcc aacatggaac
      781 ttactttaca ttctactgta aacttaaaaa ggtgaatgtc aaaacgggag ataaggtggc
      841 taccggagag cctctcggta tcctgaatga agagggtaac acctcggaaa tacatttcca
      901 gatatggaag ggaactacaa aacagaaccc agcagtatgg ctcagaccac gctgacaaag
      961 gattatttta ctccgagaat cccacgaagg tagccaccgg tataggagtt ctcgcggagt
     1021 gccacctgct cgggagtccc ttcggcaatg atatagcctc cccccttacc cccttccggg
     1081 ccaagatcta tgagataatc caccgatttg agcacatcaa gattgtgttc gataataacc
     1141 accgtattac cctgatctac cagccgtcta agtactctaa gcaggacttt tatatcttcg
     1201 aaatgaagtc cggtagtagg ctcatccaga atatagaggg tattgccggt atctctgcgc
     1261 gcaagttcgg cagagagttt cagacgctgg ctctccccgc cgctgagagt ggtactctgc
     1321 tgtccgagtt tgaggtatcc gagtccaaca tcctccagag tacgaagtct ctgcaatatg
     1381 gaaggtattg cttcaaaaaa gagacaagcc tgggaaatag tcatgttgag gagatcgttg
     1441 atgcttttac ccttatattt aaccgcaagt gtatcgggtt tatagcggag tccgttacac
     1501 tctttgcaag taacataagc agggggcagg aagttcatct ctatcacctg tactccggct
     1561 cctttgcaaa cctcgcagcg tcctcccttt acgttaaaag agaatctgtt agccttaaat
     1621 cccctgattt tggcatcggg agtctccccg aatagctttc ttatatcggt aaaaacattt
     1681 gtataagtgg ccggattgct ccgtggcgag cgccctatcg gagcctggtc tatctctata
     1741 atcttatcta tattttcaat cccacggatc gatttatatg gaagtacctt gtattttgat
     1801 ctataaaagt gattactgag aataggcatc agtgtctcat ttataagcga ggatttgcca
     1861 ctgccactga ctccgctaat ccccacaaag agacctagag acagattcaa agtgacattt
     1921 tttaaattat tgccggtggc tccctcaaga gtgatatatt ttccgttgcc tttcaggcga
     1981 acggagggta actctatgga tttatgtccg agaaggtagt cgattgtggg actttggcaa
     2041 ttacatgaag caatatcttc cggaggacca ttgtatagta actctccccc tttctctccc
     2101 gcaccggggc caagatctat cagccaatct gcattttcca tcatttgttg gtcatgttcc
     2161 accaccatta ccgagttacc ttcatcacga agggccttta atgaattgat gagctttaga
     2221 ttatctctct gatgcagacc aatgctgggc tcatcgagga tatataacac attgaccagt
     2281 ttagagccta tctgtgtcgc cagtcttata cgctgacttt ctccgccact aagtgatcgt
     2341 gtggtacggt caagcgaaag atagtccagc cctacttcct ttaaaaagcc tattcgctct
     2401 ttgagttcct tcagaatgtc ttcagctatg agacgctgcc tcggagagag tttcaagtgg
     2461 agtccgctaa cccattcata gagagtatca atctccattc ccgccacttg tgcgatattc
     2521 ttgtcatcga tacggaaaca gagcgactcg ctcttcagac gcgtaccatt gcacaccgga
     2581 cagaccattt catccacgaa gagctctttc ttgtcccaaa ccttatcgct ctcctcatca
     2641 ttatgataaa tcagcgctat cactccctga aatgaggcca tttccatatc tgagcctttc
     2701 cctatcctca ggagctcatc cgagccgtaa agaataagat ttagtgcatc ttccgaaatg
     2761 gtgctgatgg gatcatgcag cgagaagcca tattttagag caattgcctc caaatacctg
     2821 aaggtagtgt tgtccctaag catcccaaca ctctcgatac ccccggaggc tatggatttg
     2881 gaaggatccg gaattatctt gcttatatcg actgtagcga tggtacccaa acccttacag
     2941 tgcggacagg ctccctgcgg cgaattgaat gaaaaggtat ggggagccgg agttgcaaat
     3001 gagagtccgc tttcagggca aataagatta cggctcagat gctctatgga gtcatcctcc
     3061 aacctgacta ttgccagaga acctttgccc tgatggagcg ctgtaagtac cgattctctg
     3121 atacgcttac gctcctcttc gcgaggaatg agtttatcca ctaccagttc tacaaaatgc
     3181 accttgtagc gatcgagggg cttgacatcg gagagccatc taatctcccc gtctatccgc
     3241 acctgactat atcctttgcg tatgagttgt tcaaagagtt ccttatagtg acctttgcgt
     3301 ccctttacaa ggggagccag caagaggatt ttctctcctt tgtgagtttc gataataaga
     3361 tcggttatct gttcatccgt atagcgcacc atctttttcc cactctccgg tgaaaatgca
     3421 tccgctgcac gggcatatag gagacggaaa aaatcatata tttcagttat ggttccaact
     3481 gtcgaacggg gattacggcc ggtagtcttc tgctcaatgg caattatagg gctgagacct
     3541 ttgatatcct caacatcggg tctctctaga attccgataa actgacgcgc ataggctgaa
     3601 agggtctcca tatagcgacg ctgcccctca gcatagatgg tatcaaatgc aagggaagat
     3661 ttgccactgc cggagagtcc tgttaccacg accagtcggt cgcgcggaat tgacaaatca
     3721 atgttcttca gattgtggac cctcgcaccg gtaatttcga tatggtcggt gcctccgccc
     3781 ataatgtcaa cctaggaaag ggtttgtgga atattcgtat cctatggtag tctcgggacc
     3841 gtggccgggc atgaccctgg tgg
//
LOCUS       DAOSIE010000131         3853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_20816_length_3853_cov_3.961559, whole genome shotgun sequence.
ACCESSION   DAOSIE010000131 DAOSIE010000000
VERSION     DAOSIE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20816_length_3853_cov_3.961559"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PLI69_04700"
     CDS             <1..728
                     /locus_tag="PLI69_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOG41822.1"
                     /translation="DDGFGHHLWIISDKKDIARITEIFAGIPAFYVADGHHRTAAAAL
                     VGEELRRKNPNHTGEEEYNYFMAVVFPESHLKIIDYNRVVKDLNGMTPKEFIEALKED
                     FVIEKKGSAEPYRPSGLHNFSMYLEGEWYSLTAKPGRYDDNDPIGVLDVTVCSNLIFD
                     KLLDIKDLRTCTRIDFVGGIRGLGELKRRVDSGEMAAAFALYPVSMRQLINISDTGNI
                     MPPKTTWFEPKLRSGLTIHKLEK"
     gene            complement(793..1422)
                     /locus_tag="PLI69_04705"
     CDS             complement(793..1422)
                     /locus_tag="PLI69_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOG41823.1"
                     /translation="MRLSSIIIYVGVAIASAATLFSCSDGTTFTAREKQLIGTDTSAA
                     IMPLYTIEEPTEAEFLRKVAVPLTAKDVASDIYRVLRDRMLSTVTNPENQGVGIAAPQ
                     VGISRQLIAVVRFDKEGAPFEFYPNAEITFYSPEKKYGWEGCLSVPGQRDTVLRSTSI
                     VVSYNDEITFEPRTDTISGFTARIFQHEIDHLKGILYIDYKESDFVPHN"
     gene            complement(1435..2400)
                     /locus_tag="PLI69_04710"
     CDS             complement(1435..2400)
                     /locus_tag="PLI69_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG41824.1"
                     /translation="MIDNMSKEYVIGVDIGGQSAMIGIVDRRANILARSRMDTKVYGE
                     ADAEKFMDDLCANIRKISEQVDWESIEGIGIGAPNGNYYQGTIEGAANLPWGKGVVPL
                     RDYIAKKTGKPVTVTNDANAAAIGEMHYGNAKGMTNFIEITLGTGVGSGIVVNGQMVY
                     GHDGFAGELGHTTAVRHNGRQCNCGKVGCLETYASAIGVARTAREWLDMSDEPSLLRS
                     LEVITSKDVYDAAVAGDAIAKKVFEFTGQKLGESFADFVAFSTPEAIILFGGLAEAGD
                     LIMKPLVEAMEANLLSIWKGGKIKVLRSGLKESDAAILGAGALAW"
     gene            complement(2419..3696)
                     /locus_tag="PLI69_04715"
     CDS             complement(2419..3696)
                     /locus_tag="PLI69_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG41825.1"
                     /translation="MTNVKKQNFALPIAIMFALFFMIAFVTNFAGSMGVIVKNQFGAS
                     NALSQLGTLANFIAYALMGIPAGIILKRRGYKFTALTAVIVGFVGVGIQFLSGYVSPS
                     IAFGVYVFGAFVAGLSMCLLNIVVNPLLNTLGGGGNRGNQLIQFGGSCNSIGGTIAPI
                     LLGYLIGGSMESATVSDAAPAMMIAMGIFVLAGIVLGVTNIPEPHMETAEQKAARRAG
                     NVQKDKYGPMSFRHFVLGAIAIFFYVGVEVGIPNTANLYMSGNPNVGPAIAGTVVGFY
                     WFMMLVGRLVGAPIAGKVSSKKMLVSVSLLAIVFVLCILFIPETVTISFREKALPLSM
                     LFVVLCGLCTSVMWGAIFNLSTEGLGKYTPMASGIFMTLVCGGGILPFVQNWFADSVG
                     HINSYWVVVAGFAYILFYALFGNKNVNTDIVTA"
BASE COUNT         1092 a          926 c          856 g          979 t
ORIGIN      
        1 cggacgatgg tttcggacac catttgtgga taatcagcga caagaaagat atcgcacgca
       61 ttactgagat ttttgccggt attcccgcat tttatgtggc agacggtcac cacagaacag
      121 cagctgctgc tcttgttgga gaagagctac gtcgcaagaa tcccaaccat acgggcgaag
      181 aggagtacaa ctacttcatg gctgttgtat tcccggaaag ccacctcaag attatcgact
      241 acaatcgcgt tgtgaaggac ctcaacggca tgactccaaa agagtttatc gaagctctta
      301 aagaggattt cgtaattgaa aagaagggtt cagcggagcc ttaccgaccc tccggactcc
      361 acaacttctc gatgtatctt gaaggcgaat ggtactcgct tactgcaaag cccggcagat
      421 acgatgacaa tgaccctatc ggtgttctcg acgttacagt ttgttcaaac ctcattttcg
      481 acaagcttct cgacataaag gatctacgca cctgtaccag aattgacttc gtgggtggta
      541 tccgcggtct tggcgagttg aagagaaggg tggattcagg agagatggca gcagcctttg
      601 ctctctatcc ggtttccatg agacagctga tcaacatatc tgacacaggc aatataatgc
      661 ctccaaagac tacctggttt gagcctaagc tccgcagcgg tctgacgatc cacaaactgg
      721 aaaagtaaac taaaaaacgg cctcagagtg aggccgtttt ttagttatga gtattgagta
      781 aggaattttt agttagttgt gggggacaaa atcactctcc ttataatcta tataaagtat
      841 acctttcaga tgatctattt cgtgctggaa aattcttgcc gtgaaccccg aaattgtatc
      901 ggtacggggt tcgaaagtga tttcatcatt gtatgaaaca acaatagatg tagagcggag
      961 cacggtatca cgttgtccgg ggacagaaag gcaaccctcc cagccatatt tcttttcagg
     1021 ggagtagaag gttatctctg cgttcggata aaattcaaag ggggccccct ctttgtcaaa
     1081 tcttactacg gcgataagtt gtctacttat gccgacttgc ggtgctgcta ttcctactcc
     1141 ctgattttca gggttagtga ccgtactaag cattctatct ctcaaaacac ggtatatgtc
     1201 ggaagcaaca tcttttgccg tcagagggac ggcaacctta cgtaaaaatt ctgcctctgt
     1261 gggctcttca atagtgtaga gtggcattat ggcagcggaa gtgtcggtgc caatgagttg
     1321 tttttcccgt gcagtaaaag tcgtgccgtc actacaggaa aaaagtgttg cagcggaagc
     1381 aatagccact ccaacataga taataataga tgatagtctc attttaataa aaaattacca
     1441 ggcgagagcg cctgcaccaa gaatagctgc atcggactct ttgagaccgg aacgaagcac
     1501 tttaatttta ccacctttcc agatagatag aagattggct tccatcgcct ccacaagggg
     1561 tttcataata agatcacctg cctctgcaag acctccgaag aggatgattg cttcaggagt
     1621 gctgaatgca acgaaatctg caaaggactc gcctagcttt tgaccggtga attcaaacac
     1681 cttttttgca atggcatctc ctgcgacagc agcatcgtag acatctttag aggttatgac
     1741 ttcaagagaa cggagtaatg agggttcgtc actcatatct agccattcac gcgccgtgcg
     1801 cgcaactccg atagctgaag cgtaagtctc gagacaaccc accttaccgc agttgcattg
     1861 gcgaccattg tgccttaccg cagtggtgtg gcccaattcg ccggcaaagc cgtcatgtcc
     1921 atagaccatt tggccattga caacaatacc gctgcccaca ccggtcccga gagtaatttc
     1981 tatgaaatta gtcataccct tggcattgcc gtagtgcatt tccccgatag cagccgcatt
     2041 tgcgtcattt gtaaccgtca cgggtttacc ggtttttttt gcaatatagt ctctcaaagg
     2101 cacaacacct ttaccccatg gaagattggc tgcaccttca atggtccctt gatagtaatt
     2161 gccgttggga gcaccgatac cgataccttc gatactctcc cagtcgacct gctctgaaat
     2221 cttcctgata ttggcgcaga gatcatccat gaatttctcc gcatcagcct caccgtagac
     2281 cttggtgtcc attctgctcc tggcaagaat attggctcgt ctatccacga taccgatcat
     2341 ggccgactgg cctccgatat caactcctat cacatattct tttgacatat tatcaatcat
     2401 attattgtta aatgatattt atgctgttac aatgtccgta tttacatttt tgttgccgaa
     2461 aagagcatag aataggatat atgcaaatcc tgcaacaacc acccaatagc tattgatatg
     2521 ccccacggag tctgcaaacc aattttgcac gaaaggaagg atgccaccac cgcaaacgag
     2581 tgtcataaag attcctgaag ccataggggt atattttccg agaccttcgg tcgagaggtt
     2641 gaagatggct ccccacatca cagaagtaca aagaccgcat agcaccacaa agagcatact
     2701 caagggaagt gctttctcac ggaaagagat ggtaacagtc tcaggtatga agagtatgca
     2761 gagcacaaaa actatcgcca aaagtgaaac cgaaacaagc atcttcttgc tggaaacctt
     2821 accggctata ggagctccta caagccttcc aaccagcatc ataaaccagt agaaacccac
     2881 cacggtaccc gctatcgcag gtccgacatt gggattaccg gacatataca gattggcggt
     2941 attgggaata cccacctcta ctccaacata gaagaatatt gcaattgcac cgaggacaaa
     3001 atggcggaat gacatagggc catacttgtc tttctgcaca ttacctgccc tacgggcagc
     3061 tttctgctca gccgtctcca tgtgtggctc aggaatattg gtaaccccaa gaactattcc
     3121 tgcgagaacg aagatgccca tagcaatcat cattgcagga gctgcatcgc ttaccgtagc
     3181 gctctccatc gatccgccga taaggtaacc gagaagaatg ggagctatcg tccctccgat
     3241 ggagttgcag gagccgccaa actggatcag ttggttgccg cgattgcctc cgccaccgag
     3301 cgtattgagg agcggattga ctacgatgtt taggagacac atcgaaagac cggccacaaa
     3361 tgctccgaac acatatactc caaaagctat cgaaggagat acatacccgg agaggaactg
     3421 aattcctacc cctacaaaac ccactattac ggctgtaagt gctgtaaatt tatagcctct
     3481 acgcttcagg atgatgcctg caggaatacc cattagggcg taagcgatga agttggcaag
     3541 cgtaccgagc tgtgagaggg cattgcttgc tccgaactga ttttttacga ttactcccat
     3601 cgagcccgcg aaattggtca caaaggcaat catgaagaag agagcaaaca ttattgcaat
     3661 cggcagtgca aaattctgtt tcttgacgtt tgtcatattg ttgaattgtt taattttaaa
     3721 aatctttcaa atattcttca aacttacata aaaaacttca tttttacgtc agttatgtat
     3781 aaaggcgaca aaaaaggaga attacaagag taagcaatgt catttctcaa caaaagcgtg
     3841 gcaatatccc ttg
//
LOCUS       DAOSIE010000132         3785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_21263_length_3785_cov_2.168901, whole genome shotgun sequence.
ACCESSION   DAOSIE010000132 DAOSIE010000000
VERSION     DAOSIE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21263_length_3785_cov_2.168901"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLI69_04720"
     CDS             <1..448
                     /locus_tag="PLI69_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG41826.1"
                     /translation="ELVNLRISDLFLAEQFIRVTGKGSKQRLVPIGEPAVRSISLYMR
                     IRNAGPVAKGAEDILFLNRRGGKLTREMIFLIVRRQAKIAGIRKNVSPHTFRHSFASH
                     LVENGADLRVVQEMLGHESILTTEIYTHIDTKKWQQTILQFHPKRK"
     gene            518..1717
                     /locus_tag="PLI69_04725"
     CDS             518..1717
                     /locus_tag="PLI69_04725"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HOG41827.1"
                     /translation="MAYTTTTKQSYGNRVSKSFKGIGSGILLFIIGTVLLFWNEGRTV
                     KVKRALNQAADVTVEMPDISRIDPQFEGKMVHAIGDASTAEILNDGLFGISLNAINLS
                     RNVEYYQWTEQSKEEKKEKIGGGQEITTTYTYSKKWVSSPINSSDFADPDYKNSNFVL
                     TTIESNQIVADVVDFGAYKLPPFLKYGISSAEEATPELTEQMIKEWNEVLWKRDSSAT
                     VTVSTGQAYFGRDVNNPDVGDVRVTFGYTPTPQTVSILAVVVGDTFGEFVAKNGKNVS
                     RIALGSVSAANMYDAAQKENKVIAWILRIIAVILIIGGLRGIFNFLSTIFAVVPFIRR
                     VLAAGINFICTVVGLIWSLLIFVIAWISYRPALGITLLVLIAALIVLLVMRSKKKKNI
                     PADMPAQ"
     gene            1726..2946
                     /locus_tag="PLI69_04730"
     CDS             1726..2946
                     /locus_tag="PLI69_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41828.1"
                     /translation="MKQRVFLICTLIAALAAGCSTGDKTPQTTVGIKENPYKGFRKLA
                     DFGLNGPVKSFTETRYHEMQPFSRTWNFDHDGRLISIVEEDVHPQTRLEISIDKKGNP
                     VDTVFSLTDSLNYAFDMQASTYLPDGKPLKEVYKMDGEPAEIYQYIYDKNGRCIRVNI
                     VESNGDLRGSFLYFYDDNGNLTNERYVSIKYAVNGNEIISETVNVYDSYGNLTESSSY
                     NVGKLEVRDVMYYNEQGDLLRQYSEYEGGFTTEIFYTYDRDGRRNGDRFYALEDDRRV
                     IMTRDSTFIDEAGYRHEEQYTWTDGLHTSEAIFDKKGKMVEYNYYGEFAQESRIPDNT
                     IIFYYDEDGYLSKAELKSQYDVVPYYFGEKDSFGNWTRRSLPPDEVFDTYGIGIEDFS
                     HFLRRYERTYTYYD"
     gene            2983..3534
                     /locus_tag="PLI69_04735"
     CDS             2983..3534
                     /locus_tag="PLI69_04735"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOG41829.1"
                     /translation="MVTTLIVIGILAILVIWVISVQRNLISKEELCKNAMSQIGVQMS
                     SRWDALTALADLIKSYDEHEYKSLKDVIAQRTSITGSSSAADANIQETILTDAMRRIA
                     VVVEQYPNLKANEMYVKTMDSVNLYENQVRMSRMVYNDSVTNYNRLIRQIPSNIVASL
                     FGFKSKDYLEENQAKTEMPSMKK"
     gene            3534..>3785
                     /locus_tag="PLI69_04740"
     CDS             3534..>3785
                     /locus_tag="PLI69_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41830.1"
                     /translation="MKRLLLTFLTAIAAFTVTAANPAIRDIDITYILTPEGHARVTEV
                     WDVTVASGTEWYLVKYDMGSHVSITEFAVTDENGVNFINE"
BASE COUNT         1126 a          871 c          845 g          943 t
ORIGIN      
        1 cgaactggtg aatctgcgca tcagcgacct ctttttggcc gagcagttta tccgggtcac
       61 cggcaagggc tccaagcagc gtctggtacc catcggagaa cctgcagtac ggagcatctc
      121 cctctatatg aggatacgta acgccggtcc ggtagcaaag ggagcggagg atatactctt
      181 tctcaaccgc cgcggaggca agctcacgcg ggaaatgatt tttctgattg ttcgccgaca
      241 ggcgaaaatt gccggaatcc gtaaaaatgt ctctcctcac actttcagac actcttttgc
      301 ctcccatctg gtggaaaacg gagctgattt acgtgtcgta caggaaatgc tcggtcacga
      361 aagcatactc accacagaga tttacacaca tatagataca aagaaatggc agcaaactat
      421 tcttcaattc catcctaaaa gaaaataatt tttgctacct ttacaaattg gatttgaaat
      481 gattttttta ctataacact taacttatat tgaaattatg gcttacacca ctacaactaa
      541 acagagctat gggaacagag tttccaaatc cttcaagggt atcggcagcg gtatcctgct
      601 tttcatcatc ggtacggtat tgctattctg gaacgaaggc aggacagtca aagttaaacg
      661 cgcattaaac caggcggcgg atgtgaccgt ggagatgccc gatatctccc gaatcgaccc
      721 ccagtttgaa ggcaagatgg tacacgctat tggtgacgca tccaccgctg agatccttaa
      781 cgacggtctc ttcggtattt cactgaatgc catcaaccta tcacgcaacg tggaatacta
      841 ccagtggacc gagcagagca aagaggaaaa aaaggagaaa ataggaggcg gtcaggagat
      901 cacaaccacc tacacttata gcaagaaatg ggtttcttcg cccataaact cttcagattt
      961 cgctgatcct gattataaga actccaactt tgtgctcacc actattgagt caaaccaaat
     1021 tgtggctgat gtagtagatt tcggagccta caaactccct cctttcctaa agtacggtat
     1081 cagtagtgca gaagaggcca ctcccgaact caccgagcag atgatcaaag agtggaatga
     1141 ggtactctgg aaaagagaca gctctgctac agttactgtg agtacgggtc aggcctattt
     1201 cggacgcgac gtcaacaacc ctgacgtggg cgatgtcagg gtaacattcg gatatactcc
     1261 tactcctcaa acagtctcca tactcgcggt agttgtcggt gatacattcg gtgaattcgt
     1321 ggctaaaaac ggtaaaaatg tctcacgcat tgcactcggc tcagtttctg ctgcaaatat
     1381 gtatgatgca gcacagaaag agaataaggt aattgcctgg atcctccgta tcattgccgt
     1441 tatattgatt attggcggac tcaggggcat attcaacttc ctcagcacca tattcgcggt
     1501 tgtaccattt atacgcaggg ttctcgctgc cggcatcaac tttatctgca ccgtcgtcgg
     1561 tcttatctgg agtctattga tatttgtcat cgcatggata tcctaccgtc ctgcactcgg
     1621 cattacactc cttgttttga tagccgccct catagtgcta ctggttatgc gttcaaagaa
     1681 aaagaagaac attcccgctg acatgcccgc ccaataatcg cagacatgaa gcaaagagta
     1741 tttttgattt gcacactgat agcagctttg gcagcgggct gttcaacagg agataaaact
     1801 cctcagacta ctgtcggcat taaagagaac ccatataaag gttttagaaa gcttgccgat
     1861 ttcggactca acggtcccgt caagtcattc accgagacca gataccatga aatgcagcct
     1921 ttctcacgta catggaattt tgaccatgac ggcaggttaa tctcaattgt cgaagaagat
     1981 gtgcaccccc agacacgtct ggaaataagt atagataaga aaggaaatcc tgtcgacacg
     2041 gttttttcct tgacagattc tctaaattat gcctttgata tgcaggccag cacttatctg
     2101 cccgacggta agcctctcaa agaggtctat aaaatggatg gcgaaccggc tgagatctat
     2161 caatacatct acgacaagaa cggccgctgc ataagggtca acatagttga gagcaacggt
     2221 gatcttagag gctctttcct ctatttctac gatgacaatg gcaacctgac aaacgaacgc
     2281 tatgtaagca tcaaatatgc cgtaaatggt aacgaaatca tatcagagac cgtaaatgta
     2341 tatgatagct atggtaatct gactgagtcc agcagctaca atgtcggcaa actcgaagtg
     2401 agagatgtaa tgtattataa tgagcaagga gacctgttac gccaatattc cgagtatgag
     2461 ggaggattta ccaccgagat tttctacaca tacgacagag atggcaggcg caacggtgac
     2521 aggttctatg ccctcgagga tgaccggcgt gtgataatga ccagagatag cacgttcatt
     2581 gacgaagccg gatacagaca tgaggaacaa tacacctgga cagacggtct tcatactagt
     2641 gaagccattt ttgacaaaaa gggaaaaatg gtggaataca attactatgg cgagtttgcc
     2701 caggaatcac gcattccaga caataccata attttctatt atgatgagga tggctacctc
     2761 agtaaagcag agctgaagag ccaatatgat gtagtcccct actattttgg agaaaaagac
     2821 agtttcggca actggacccg tcgctccctt ccccctgatg aggtatttga tacctacggc
     2881 atcggtatag aagatttcag ccatttcctt cgacgctatg aaagaactta tacctactac
     2941 gactaaaaca caaatattta tcaacacata aaactttatt ctatggtaac aacattaatt
     3001 gtgataggca tccttgccat tttggtaatc tgggtaattt ccgtacaacg taatctgatc
     3061 agcaaggaag aattgtgcaa aaacgcaatg agccagatag gcgtccaaat gtcgagccgc
     3121 tgggatgcac ttacggcact cgcagacctc atcaaatcct atgacgaaca cgagtacaag
     3181 tcactcaaag atgtaattgc acagcgtaca agtattaccg gcagcagttc ggcagccgat
     3241 gccaacatac aggaaactat cctgacagat gccatgagac gtatagccgt ggtagttgag
     3301 cagtacccca atctcaaagc caacgagatg tacgtcaaga ctatggatag tgtcaatttg
     3361 tatgaaaatc aggtacgtat gagccgtatg gtatataatg acagcgtaac caactacaac
     3421 cgcctcatcc gtcagattcc cagcaatatc gtagcttcat tgtttggctt caagtccaag
     3481 gattatctcg aggagaatca ggccaagact gaaatgccct caatgaaaaa gtgatgaaac
     3541 gtttgttgct cacatttttg accgctattg ccgctttcac tgtgacggcg gcaaatcctg
     3601 ctattcggga tattgacata acatacatac ttacgcctga aggccatgca cgagtgaccg
     3661 aagtgtggga tgtcacagtt gccagcggca ccgaatggta tctggtcaaa tatgatatgg
     3721 gatcgcacgt atcgattaca gagttcgcag taaccgacga gaatggtgtt aatttcataa
     3781 atgaa
//
LOCUS       DAOSIE010000133         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_21350_length_3773_cov_4.488166, whole genome shotgun sequence.
ACCESSION   DAOSIE010000133 DAOSIE010000000
VERSION     DAOSIE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21350_length_3773_cov_4.488166"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..872
                     /locus_tag="PLI69_04745"
     CDS             159..872
                     /locus_tag="PLI69_04745"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOG41831.1"
                     /translation="MDTIQDINVMPRIGDKAPAFKAVTTQGNINFPADFEGKWTILFS
                     HPADFTPVCTSEFMTFATMQKEFDDLNCQLIGLSIDGLYSHIAWLRTIKEKIEYKGMK
                     NVEVTFPLIEDITMEIAQKYGMVQPGESTTKAVRAVFFIDPKGIIRTIIYYPLSLGRN
                     FKELKRVLIGLQTADKFGVALPADWHPGDDVIVPTAGSCGTAKARMEGKDGEMTCYDW
                     FFCTKKLSKEEVEKKLYKK"
     gene            938..1261
                     /locus_tag="PLI69_04750"
     CDS             938..1261
                     /locus_tag="PLI69_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOG41832.1"
                     /translation="MSRPKKCRKIRSSPEVMCFKPVGVPLRDLERVIIEHDEYEAFRL
                     AAYDRLLQEEGAERMEISRPTFTRIYNCAVQKIAQAIIEGKIIDINCCEEVLKQIKGS
                     DNSAL"
     gene            1312..1587
                     /locus_tag="PLI69_04755"
     CDS             1312..1587
                     /locus_tag="PLI69_04755"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOG41833.1"
                     /translation="MPRFDKTGPIGEGPMTGRNKGKCKNFDDKKGFVEETELENLNAD
                     QGRGMGSGLGRGRRGCGGGMGRTSGRRCGSGLRSGAGPGRGDGKCRE"
     gene            1601..1933
                     /locus_tag="PLI69_04760"
     CDS             1601..1933
                     /locus_tag="PLI69_04760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG41834.1"
                     /translation="MKIQHRLEDNRRGSFFIMDKERQIAELDYDLNNGVLNAYHTGVR
                     PELEGQGIAGMLFDELVKYARDNNYKVIPTCSYILVKFRRNPEKYSDIWFRMDNEPSG
                     TCCGVRPK"
     gene            1990..3672
                     /locus_tag="PLI69_04765"
     CDS             1990..3672
                     /locus_tag="PLI69_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG41835.1"
                     /translation="MEKKRIIVIGGSAAGPKAASRARRLDENAEITIIQKAPDLSMAS
                     CGYPYYVGGFFDDRNQLLTSPAGVVRDSKFFWNAKRIKALVNTEVTQIDRKNKSVEYK
                     DLVTGEAASLAYDKLIIATGATPRKPPVPGVDLKGVTTLQSMADADFLRQVGDEGEVQ
                     KAVVIGGGLIGIETCEALHLSGIEVTLVEMLPQLLAFLDKGMARIVEKYVQTKVNLIL
                     QNGVSEFLGENGKLKAVKLSDGSEIACDIAVVATGVRPNSALAKEAGLTIGEFGAIEV
                     NTYMQTSDPDIYAIGDCIEIPNLISGKKVHAPFGDLANLQGRVAGTNVINGNSAQFPG
                     TIQSGICKVFDYGVGSTGLSEQNAARHGFDNIETVVNASPDKPGFMGAKLLITKLVVD
                     KNTNRILGAQVLGPGDVGKQVAIWAMAIKGKLTVEDMINADLPYAPPFSLAIDHSIAT
                     AHIMQNKLNRLFVGITAEQLKEKLDKKDPMFLLDARNPNEYEAQKLGIGETLIPLGQL
                     RNRLAELPADKNAEIITWCKISLRGYEAARVIMANGYKNVKVLEGGLNAWPF"
BASE COUNT         1192 a          690 c          883 g         1008 t
ORIGIN      
        1 atccataatc aattaatgat tctcggatat attattttta aaaattaaaa ataatatcag
       61 aaaaatttgg attatgaaca aaggttcatt atctttgtag aaattttaca aataacaaga
      121 agaaatttat taataattaa acatttaaac ctactattat ggatacaatt caagacatta
      181 atgtaatgcc gcgcattggt gataaggcgc ctgcattcaa agccgttacc actcaaggta
      241 acataaattt tcccgctgat ttcgaaggga aatggacaat cctgttcagc caccccgctg
      301 actttactcc cgtttgtact tcggagttca tgacatttgc aacaatgcaa aaagaatttg
      361 atgatcttaa ttgtcagctg atcggtttgt caattgacgg tctatacagc cacattgcct
      421 ggttacgtac catcaaggaa aaaatcgagt acaagggaat gaaaaacgtg gaagttactt
      481 ttccgctaat agaagacata actatggaaa ttgctcaaaa atatggcatg gttcaaccgg
      541 gtgaaagtac taccaaagca gttcgtgcag tatttttcat cgacccgaag ggaataatcc
      601 gcacgattat ctattatcct ttgtcattgg gacgcaattt taaggaactg aaacgcgtac
      661 ttatcggtct tcaaacagcc gacaaatttg gcgttgcttt gcccgccgat tggcatccgg
      721 gtgatgatgt tatcgtaccg actgccggtt cttgcggaac ggcaaaagcc cgtatggagg
      781 gtaaggatgg agaaatgacc tgctatgact ggttcttctg taccaagaag ctttcaaaag
      841 aagaagtaga aaagaaacta tacaagaaat agttgactta attaactttc ataacccgta
      901 catatgtgcg ggttatgatt aatattttat agtaataatg tcgagaccta agaaatgtcg
      961 aaaaatacga agttcaccgg aagtgatgtg tttcaagccg gttggagttc cgttgagaga
     1021 cttggagaga gttattatcg aacacgatga atatgaggcg ttcagattgg ctgcttacga
     1081 cagattgttg caggaagagg gtgcggaaag gatggaaatt tctcgaccca cttttacgcg
     1141 aatctataat tgtgcagttc aaaaaatagc acaggcaatt atagagggta aaattataga
     1201 catcaattgt tgtgaggaag ttcttaaaca aataaaaggg agtgataata gtgccctgta
     1261 atgcctgtga agtagatgta atttattaac aatttaaaat atggagttta tatgccacga
     1321 tttgataaaa caggaccgat aggggaaggc ccgatgaccg gtcgtaacaa gggaaaatgc
     1381 aaaaattttg atgataaaaa gggttttgtt gaagagacgg aacttgagaa tctaaatgcc
     1441 gatcagggac gaggtatggg cagtggtttg ggccgaggtc gacgcggatg cggtggagga
     1501 atgggaagaa catccggacg ccggtgcgga tccggactga gatcaggagc aggtcccggt
     1561 cgcggagatg gaaaatgccg cgaataatat tattcaatat atgaaaatcc aacacagact
     1621 tgaagataat aggagaggat ctttcttcat tatggataaa gagaggcaga tagccgaact
     1681 cgattacgac ctgaataatg gtgttttaaa tgcatatcat accggagttc gtcctgaatt
     1741 ggaaggacaa ggcattgcag gaatgctttt cgatgaattg gtaaaatacg caagagataa
     1801 taactataag gtaatcccca cttgctctta tatactggtg aaattcagac ggaatcccga
     1861 aaaatatagc gatatctggt ttcgcatgga caatgaaccc tcaggcactt gttgtggggt
     1921 cagacccaaa taatttttaa gtatccaatt atatcacaaa acatttaaaa tatttaacgt
     1981 aaaataatta tggagaaaaa aaggattatt gttatcggtg gttctgctgc cggccctaag
     2041 gctgcttcaa gagcgcgtcg tctggatgaa aatgccgaga ttaccattat tcaaaaggcc
     2101 cctgacctct cgatggcttc ctgcgggtat ccctattatg taggtggttt ctttgacgat
     2161 cgcaaccaac tcttgacctc tccggccgga gtagttcgcg attccaaatt cttttggaat
     2221 gccaagcgaa ttaaggctct ggtaaatacg gaagttaccc aaattgacag aaaaaacaag
     2281 agcgtagagt acaaagattt ggttacggga gaagctgctt cgttggcata cgacaaactg
     2341 ataattgcaa ccggagctac tccccgcaaa ccgcctgtgc cgggtgttga tttaaaggga
     2401 gttaccacgt tacaatcaat ggctgatgca gatttcctga gacaagtggg agatgagggc
     2461 gaagtccaaa aagccgtggt aataggcgga ggtcttatcg gtattgagac ctgtgaagct
     2521 ttgcatctgt caggaatcga ggttacactc gttgagatgt tgccgcaatt gcttgcattc
     2581 cttgataaag gcatggcacg aatcgtagag aaatatgttc aaaccaaggt gaatttaatt
     2641 ctccaaaacg gagtttccga gtttcttgga gagaatggaa aactaaaagc agtaaaactt
     2701 tcggatggtt ccgaaatagc atgcgatatt gcagttgtag ctaccggagt cagacccaat
     2761 tccgcattgg ccaaagaagc aggattaaca ataggagagt ttggagccat tgaggttaat
     2821 acctatatgc agacttccga tcccgatatt tatgccatcg gtgactgtat tgagattcca
     2881 aaccttattt ccggtaaaaa agttcacgct ccttttgggg acttggccaa tctgcaaggt
     2941 cgagttgcag gtaccaatgt gataaatgga aactccgctc aattccccgg aactatccaa
     3001 agcggtatct gtaaagtatt tgattatgga gtgggctcaa caggactttc ggaacaaaat
     3061 gctgccagac acggatttga taatattgaa acggttgtaa atgcaagtcc ggacaaaccc
     3121 ggcttcatgg gagccaaact tctgattacc aaactggttg tggataagaa cactaaccgt
     3181 atcctcggtg cgcaggtact tggcccgggc gatgtcggca aacaagttgc catatgggcg
     3241 atggccatta aaggtaaact tacagtcgag gatatgataa atgccgattt gccgtatgct
     3301 ccacctttct ctttggcgat agatcacagc attgccactg cacacataat gcaaaacaag
     3361 ttgaatcgcc tttttgtagg aattactgca gaacaactca aagagaaatt ggataaaaag
     3421 gatcctatgt ttttgttgga tgcccgtaat ccgaatgaat atgaagcgca aaagcttgga
     3481 ataggggaga cgttgatccc tctcggacaa ctgagaaatc gcttggcaga attacctgca
     3541 gataagaatg ctgagatcat cacctggtgt aaaatctccc tacgcggata tgaagcagcg
     3601 agggttatta tggcaaacgg gtataaaaat gtaaaagtac tcgaaggcgg ccttaatgct
     3661 tggccttttt aaagttttag attgttctta ttaaaataaa accggcggca gactttgctc
     3721 tgccaccggt tttgtattgt tatgaatagt tttggagttt aaatcaaaac taa
//
LOCUS       DAOSIE010000134         3769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_21378_length_3769_cov_4.149165, whole genome shotgun sequence.
ACCESSION   DAOSIE010000134 DAOSIE010000000
VERSION     DAOSIE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21378_length_3769_cov_4.149165"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLI69_04770"
     CDS             complement(<1..241)
                     /locus_tag="PLI69_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41836.1"
                     /translation="MKRLTAFIAFTTIILLSLVSCNQGKGDYALSVSSEIRTTDPVVI
                     TVPDAYVINVGDNTPDELKKLIQITPKREFSVSLID"
     gene            298..2604
                     /gene="pbpC"
                     /locus_tag="PLI69_04775"
     CDS             298..2604
                     /gene="pbpC"
                     /locus_tag="PLI69_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOG41837.1"
                     /translation="MLKSKKKKIFIALCCAATLAALVIIFFPRQIFHKPVSALLESSD
                     GRVLGARIASDGQWRFPQIDTVPDKFARAIITYEDKRFRYHLGVDPLAIGRAILLNMR
                     KREIRSGASTITMQTIRLSRENRPRTIGEKIIEMLQAVRLELRYSKTKILAMYASNAP
                     FGGNVVGLEAASWRYFGRPAGELSWAESAMLAVLPNSPALIHPGKNRELLLEKRNMLL
                     DKMASKGIIDATECMLSKEEPLPDKPYPMPDIAYHYLETLRKEGGDRRYVTDIDKGLQ
                     IRTEEILRRHVTLFESNQVYNIAALVMEVNTGHIITYCGNLNPEGSGAGHGRAVDVIQ
                     APRSSGSTLKPFLYAAMLDDGEILPTMLVPDIPSHYKDFTPSNFNHTFDGAVPANSII
                     RRSLNVPSVRLLQEYGVEKFLLLLKEMGFTTVNRSAEDYGLSLILGGAEIRLIDLVHA
                     YRDMAATLAESKEQLSPLSTGAIWCTFDVLTGVSRPEEEGSWQSFSSSRTVAWKTGTS
                     YGNRDAWSIGVTPEYVVGVWVGNCNGEGRPNLTGIGYAAPVMFEILSILPPTGWFELP
                     ILDLEEVAVCPLSGHPVSDICSIGTGLTPDTLLVPPHDHRPDVCPYHRLLHLSEDQRY
                     IVNSDCYSTAKMVTRSWFVLPPSQEWYYMATHPDYRRLPPLHPNFASGGGSSRGKIVE
                     IIYPQEGMVLAAPLSLDGKSQGVVFSAAHASPSATLYWHIDDRYIGSTTSNEHKMLII
                     PEPGQHRLVVTDTNGNSASVWFEGAQAN"
     gene            2685..2761
                     /locus_tag="PLI69_04780"
     tRNA            2685..2761
                     /locus_tag="PLI69_04780"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2719..2721,aa:His,seq:gtg)
     gene            complement(2815..3639)
                     /locus_tag="PLI69_04785"
     CDS             complement(2815..3639)
                     /locus_tag="PLI69_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41838.1"
                     /translation="MKKNRIFPVLLLVAVFLTSCDWMWWINPGPPEPENLFLTGECLG
                     STELVYVPYYASRDDDYLEKWKCFPFVFHLDTANVQRYKDRPLFKSDPAFHIPSDELF
                     KSFGKKSFVKSDFFDSFEKIREGRSYFEITTILYDGGLSLTADVDFAGHPAGEELAPF
                     IVSHPSVNHPSSWTGVCRTIEPLLSAPGNTKEKLRPILDIPLDYECLLESGIDIGLPV
                     GDYTRVVERVTFTLKIPVRVVYYLQWLEDKHSNPNAPVPYKDEVLTCKFYTDIGFR"
BASE COUNT         1034 a          905 c          864 g          966 t
ORIGIN      
        1 catcaataag cgatacgcta aactcccttt tgggagtaat ctggatcaac tttttcagtt
       61 catcgggcgt attgtcgcct acattgataa cataagcatc aggtactgtt atgactacgg
      121 ggtctgtagt cctgatttcc gaagatacgg ataatgcgta atctccctta ccctgattac
      181 aggaaaccag ggataggaga ataatggtcg taaatgcgat aaaagcagtc agtcgtttca
      241 tattttttcg gttaattgtt atatttgcaa atgtactgaa aaatcgtctc cattcaaatg
      301 ctaaaaagca aaaaaaagaa aatatttata gcactttgct gcgccgcaac ccttgcggcg
      361 cttgttatta tcttcttccc ccgacagatt tttcataaac cggtcagcgc attacttgaa
      421 agctcagacg gaagagtgct gggagccaga atcgcctctg acggacaatg gcgatttcca
      481 cagattgata cagtacccga caaatttgcc cgcgccatca taacatacga agataaacgg
      541 ttcagatacc atctgggggt ggaccctctg gccataggaa gggctattct actcaatatg
      601 cgcaaacgag agattcgtag cggcgccagc accattacga tgcaaaccat acgcttgagc
      661 agggaaaatc gtccccgcac aattggggaa aaaattatcg aaatgctcca ggcggtgaga
      721 cttgaactgc gctattccaa aaccaaaatc ctggcaatgt atgcctctaa cgcccccttc
      781 ggaggcaatg tggtaggact tgaggcagcc tcctggcgtt atttcggccg cccggccgga
      841 gaactctcct gggctgagag cgccatgctg gccgtacttc ccaactctcc cgcattgatc
      901 catcccggca aaaaccggga actcctgctg gaaaaaagaa atatgctatt ggacaaaatg
      961 gcctccaagg gcataataga tgctactgaa tgcatgctct ccaaagagga acctctgcct
     1021 gacaaaccat atcccatgcc cgatattgca taccactacc ttgaaactct ccgcaaagag
     1081 ggaggtgacc gcaggtatgt cactgatatt gacaaaggac tccagatacg caccgaggag
     1141 attctccgcc gacatgtgac actattcgag agtaatcagg tctataatat tgcagccctt
     1201 gtaatggaag tgaacaccgg tcacatcatc acatattgtg gcaatctgaa ccctgaaggc
     1261 agcggtgcag gccatggtag ggctgtggat gtgatacaag cgcctcgcag cagcggaagc
     1321 actctaaaac cttttctata cgctgcaatg cttgatgacg gagagatact tcccacgatg
     1381 cttgtgcctg acattccctc acattacaag gatttcactc cgagtaactt taaccacacc
     1441 ttcgatggtg cagtacctgc aaatagcatc ataagacgct cgctcaacgt accctcagta
     1501 aggctgttac aggagtatgg tgtggagaag ttcctgttgc tcctcaaaga aatgggattt
     1561 actacggtca accgcagtgc agaagattat ggcctgtccc taattctagg aggtgcggaa
     1621 atacggctca tagaccttgt gcatgcctac cgcgatatgg ccgctacact tgcagagtct
     1681 aaggagcagc tctcacctct ctccacggga gcaatatggt gtactttcga cgtcctcacc
     1741 ggagtcagca gacccgagga agaagggagt tggcaaagtt tcagttcatc acgcacggta
     1801 gcctggaaga cgggtaccag ctatggaaac agggatgcct ggagcatagg tgttactccg
     1861 gagtatgttg tgggagtctg ggtaggcaac tgtaacggcg agggacgacc caatcttacc
     1921 ggtatcggct atgctgcacc tgtaatgttt gaaatactct ccatactgcc acctaccgga
     1981 tggtttgaac tgccgatact cgaccttgaa gaggttgctg tctgccccct tagcggacat
     2041 ccggtatccg atatttgctc tattggtacc ggtcttactc ctgatacgct tttggtaccc
     2101 ccgcatgatc ataggccgga cgtctgtcct tatcaccgtt tactccacct cagtgaggac
     2161 caaaggtata ttgtcaatag cgactgttat agtacggcta aaatggttac acgctcgtgg
     2221 ttcgtgttgc ctccttcgca ggaatggtat tatatggcca cgcatccgga ctaccgcagg
     2281 ctgcctcccc tccaccctaa ttttgcatcg gggggaggtt catcgcgcgg caagattgtg
     2341 gagataatct atcctcagga gggaatggtg ttggcggctc ccctttctct cgatggaaaa
     2401 agtcagggag tggtattctc agcggcacat gccagtccat ctgctaccct gtactggcat
     2461 atagacgatc gctatatagg ctccacaacg agcaacgaac acaagatgct gatcatccct
     2521 gagcccggac aacaccgtct ggtggtgacc gacactaacg gcaatagtgc ttccgtctgg
     2581 tttgaaggtg cgcaggccaa ttgacaaagc cttttttcca ttatttgttg aataatccga
     2641 aagaatgtct atatttgcaa tcccttccga acaaagggta tatggtggta gtagctcagc
     2701 tggttagagc atcggattgt ggttccgagg gtcgtgggtt cgaatcccat cttccacccc
     2761 aaaagagaaa agcaccgtaa tggtgctttt ctcttttggt gggttgctcc gctcctatct
     2821 aaaaccaatg tctgtataaa atttacaggt tagcacttca tctttgtagg gaactggggc
     2881 gttagggttg ctgtgtttat cttcaagcca ttgtaaatag tatacaaccc tgacggggat
     2941 cttcagtgtg aatgtaaccc tttcaactac gcgtgtgtaa tcacctacag gaagaccaat
     3001 gtcaattccg gattccaaaa gacattcata atccaaagga atgtcaagaa ttggcctcaa
     3061 tttctctttc gtatttcccg gggctgaaag aagaggctcg attgtccggc aaacccctgt
     3121 ccaagaagaa gggtgattga cactgggatg acttacaata aaaggggcaa gttcttcacc
     3181 agccggatgt ccggcaaaat caacatccgc agtcaaagag agaccgccat catataagat
     3241 ggtcgtgatt tcaaaataac ttctgccttc tcttattttc tcaaatgaat caaagaagtc
     3301 cgactttaca aatgactttt taccgaatga cttaaacaac tcatcagatg ggatatggaa
     3361 ggccggatcg gatttaaaaa gaggacggtc tttatacctc tgaacgtttg cggtatccag
     3421 atggaacacg aaagggaaac atttccattt ttctaagtaa tcatcatccc tggaagcata
     3481 atatggaacg tataccagtt cagtactccc cagacactct ccggtgagaa acagattttc
     3541 aggttcaggc ggacccggat ttatccacca catccaatca caagatgtta aaaacacagc
     3601 tactaaaagt agtaccggaa aaattctatt ttttttcata actaatggtg cttgacggat
     3661 tgttgcatca atatttgcgt gtcagcaaag atgattccga tatttatctt gcaaatttaa
     3721 gaaaatccat caaatttgca atcattaatc atattttttt gcaacatca
//
LOCUS       DAOSIE010000135         3753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_21507_length_3753_cov_3.594916, whole genome shotgun sequence.
ACCESSION   DAOSIE010000135 DAOSIE010000000
VERSION     DAOSIE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21507_length_3753_cov_3.594916"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLI69_04790"
     CDS             <1..285
                     /locus_tag="PLI69_04790"
                     /inference="COORDINATES: protein motif:HMM:NF028271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BT1926 family outer membrane beta-barrel
                     protein"
                     /protein_id="HOG41839.1"
                     /translation="PWNQPATGSGTQFDDIALYRPALRGAQVLGFGGGLTAGVSYSLA
                     QGLILSFEVAPVLYQYSLLHLQVQGQDAYYAMNHNIRAFAFPQLKLGIRF"
     gene            complement(392..1252)
                     /gene="rsmA"
                     /locus_tag="PLI69_04795"
     CDS             complement(392..1252)
                     /gene="rsmA"
                     /locus_tag="PLI69_04795"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOG41840.1"
                     /translation="MSKPLEQTRKPVVRPKKGLGQHFLTDQTIARNIVGALKGAGDGD
                     MDGCNNSFDALEIGPGMGVLTQYLLARSDLRLKVIEIDGESVTYLLEKWPQLSPVLYE
                     ADFLQTNLSKLFKGKFSVIGNFPYNISSQIFFKILDYKEQVPEVVCMIQKEVAERIAA
                     PPGSKTYGILSVLLQAWYDIEYLFTVGEGAFVPPPKVKSAVIRLTRNSRTKLDCDERV
                     FKMVVKTAFNQRRKKLSNSLKPLIEDSVKQERGSLLEPQVDECVFADARPEQLSVEQF
                     IILTKALQRQ"
     gene            complement(1245..2378)
                     /locus_tag="PLI69_04800"
     CDS             complement(1245..2378)
                     /locus_tag="PLI69_04800"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG41841.1"
                     /translation="MKKITLFALVALLLTACAPKSYMEFENDLNAFREEVGNVGLGVA
                     FVKENKLIYDNYFGVKNLETGEKIDAQTIFRIASISKSFTATALMQLVEQGKVTLDTD
                     VSDLMGFRVRNPKYPETVITLDMLLSHTSSLSDSLGYFTLDVINPATNPEFAGCYNDY
                     EPGKGYRYCNLNFNMAGTILEKLSGERFDQYVVNHILNPLGLYGGYDIDALDPTRFAT
                     LYSYNSENDSLEAQPNAYKSRAEEIANYTMGYTTPIFSPTGGMKISTTDLAKYMMMHM
                     NYGLYPGAGERIISEESSRNMQTPRSDDEHYGLALWRNSDYIPEVELVGHTGGAYGLR
                     SAMFFHPEEKYGFIVISSGTKSPQDTILQGTIKIMYDHFIANE"
     gene            2464..>3753
                     /locus_tag="PLI69_04805"
     CDS             2464..>3753
                     /locus_tag="PLI69_04805"
                     /inference="COORDINATES: protein motif:HMM:NF017043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG41842.1"
                     /translation="MKEKKSATPLLKQFYEIKAQYPDSLLLYRVGDFYETFGEDAVIA
                     SKVLGIVLTRRASGKGSYIELAGVPHHALDSYLPKLVQAGYKVAVCDQLEDPKLTKKL
                     VKRGVTELVTPGVAYNDTILKQNEHNYLAALSFEGKSAGIALLDISTGTFKVAQGSLE
                     YMENLLSGSAPKEILVERSYCDGFRKRFGNIAYISPLDEWAFVLESCQKKLRSHFNIS
                     SLKGFGIEGMPLAIEAAGAILFYMEITHHHSLGHVTSISRIDEGNFVWIDKFTFRNLE
                     IFSPIAGAEGVSLLETIDRCSSPMGARRLREWLAMPIKDISELNLRYDTISAFVADEK
                     MLEKVRSRIGDIGDLERIISKASAGKLLPREALQLSRGLDAIAEIRKLLLNSSNPVFS
                     SCIEQLDACGELSKSISTRILPDTAAQIGKGDVIARGV"
BASE COUNT          913 a          887 c          957 g          996 t
ORIGIN      
        1 ccctggaatc agcccgctac aggcagcggg acgcagttcg atgatatcgc gctgtatcgt
       61 cctgccctca gaggcgctca ggtattagga tttggcggtg gactgacggc aggcgttagc
      121 tactcattgg ctcagggact tattctgagc ttcgaagtag ctcccgttct ctatcagtac
      181 tcgctattac acttgcaggt gcagggacaa gatgcatact atgctatgaa tcacaacatc
      241 agagcgtttg cttttcctca attgaagctt ggcatccgct tctagtctga tcatcagcca
      301 gtagcattac taaaacttaa aaaggcgacc aaacggtcgc ctttttctgt ctatcttatt
      361 tccctccgga catcccgccc gtgtgacctt cttattgacg ctgcaatgct ttcgtgagta
      421 tgatgaattg ttccacggag agttgctcgg gccttgcatc tgcaaatacg cactcatcaa
      481 cttgaggctc caggagagat ccccgctcct gctttacgga atcttcaata agaggcttga
      541 gggagttgct gagttttttg cggcgctgat tgaaagcggt ctttaccacc attttgaaaa
      601 ctctctcgtc gcaatcgagc ttagtacggc tgttgcgggt gaggcggatg acggccgatt
      661 tgacctttgg aggaggtaca aatgcccctt ctcctacagt gaagaggtat tctatatcat
      721 accaggcctg aagcagcacg gagaggatac cgtaggtttt agagccggga ggggcggcaa
      781 tacgctcggc cacctccttc tgtatcatac agaccacttc gggtacctgt tccttataat
      841 cgaggatttt gaagaatatc tgtgaggaga tattgtaggg aaaattacct atgaccgaaa
      901 actttccctt gaacagtttg gatagattgg tttgcagaaa gtcagcctcg taaaggacgg
      961 ggctgagttg cggccatttt tccagcagat atgttaccga ttcgccatcg atctctatca
     1021 ctttcaggcg caggtcgctt cgcgcaagca gatattgtgt aagcactccc atcccgggac
     1081 ctatctccag cgcatcaaaa ctattattgc agccatccat atcaccatcg ccggcaccct
     1141 tcagagcacc gacgatgttg cgcgctatgg tctgatcggt aagaaaatgc tgtccaagcc
     1201 cctttttagg ccttacaaca ggttttctcg tttgctcgag gggtttactc atttgcgatg
     1261 aaatgatcgt acatgatctt tatcgtgcct tgtagtatgg tatcctgtgg tgatttcgta
     1321 ccgctgctga ttacgatgaa gccatatttt tcctcagggt ggaagaacat tgcacttctg
     1381 agcccatagg caccgccggt atgacctaca agttccactt caggtatata atcactattg
     1441 cgccacaggg ccagtccgta atgttcatca tccgagcggg gagtttgcat gttcctggag
     1501 ctctcctccg atatgatgcg ctcccctgct ccggggtaga ggccgtaatt catgtgcatc
     1561 atcatatatt ttgcaagatc ggtggtggag atcttcattc ctccggtagg ggagaatatc
     1621 ggagtggtgt aacccatagt gtagttggca atctcctcag cccttgattt atatgcgttg
     1681 ggctgggctt cgaggctgtc attttccgaa ttgtatgagt atagggtggc aaagcgagtc
     1741 gggtcgagag catcgatgtc gtagcctccg taaagcccga ggggattgag tatatggttt
     1801 acaacatatt ggtcaaagcg ttctcccgag agcttttcca gaatggtacc ggccatattg
     1861 aaattgaggt tgcagtaacg atagccttta cccggctcat aatcgttgta acagccggca
     1921 aactccgggt tggttgccgg attgatcacg tccagagtaa agtaacctaa agagtcactt
     1981 aggctagagg tgtgtgagag cagcatatcg agagtgatga ccgtttcggg atattttgga
     2041 ttgcgtaccc ggaatcccat cagatcgctt acatccgtat caagggtcac ctttccttgt
     2101 tcaaccagct gcataagggc tgtggcggtg aaggatttgg agatggaggc aatcctgaaa
     2161 atcgtttgtg catctatctt ttctccggtt tcgaggtttt taactccgaa atagttgtcg
     2221 tagatgagct tattctcttt gacgaaggct actccgaggc cgacgttgcc cacctcctct
     2281 ctgaaagcgt taaggtcgtt ttcaaactcc atgtaggatt tgggggcgca agccgtaagc
     2341 agaagcgcaa ccagcgcaaa tagggtgatt ttcttcataa tcataggttt tgttaaatta
     2401 tttttacaaa gatacgattt caaagttata tttttcccaa gataattggt atttttgtgg
     2461 actatgaaag aaaagaaatc agctactccg ctgctaaagc agttttatga gatcaaggcg
     2521 caatatcctg actctctgct cctctacagg gtgggtgatt tctatgagac tttcggtgaa
     2581 gatgccgtta ttgcgagcaa agtcctcggc atagtcttga cgaggcgcgc aagcggcaag
     2641 ggaagttata tcgaattggc gggagtgcct catcacgcat tggacagtta tcttccaaaa
     2701 ctggtacagg ccggatacaa agtggcggtt tgcgaccagc tggaggatcc gaagctcacc
     2761 aaaaaactgg tcaaacgcgg tgttacagag cttgtaactc ccggagtggc ctacaatgat
     2821 accattctaa agcaaaatga gcacaactat ctggcggcac tctcttttga agggaagagc
     2881 gcaggtattg ccctgctcga catctccaca ggcactttca aagtagccca aggctctctt
     2941 gagtatatgg aaaatcttct ttcgggcagc gccccgaaag agatcctggt ggagaggagc
     3001 tattgcgacg gtttccgtaa aagatttggc aatatcgcct acatctcccc actcgacgag
     3061 tgggccttcg tcttagagtc gtgccaaaaa aaactccgca gccatttcaa cataagttcg
     3121 ctcaaaggat ttggcataga gggcatgccc ctcgccatag aagcggcagg tgcaattctc
     3181 ttctatatgg agattaccca ccaccattcg ttggggcacg tgacttcaat ttcccgtata
     3241 gatgaaggga atttcgtctg gatagacaag tttacattcc gcaacctcga gatcttcagc
     3301 cccattgccg gagcagaggg agtgtcgctg ctggagacta tcgacagatg cagctcgcct
     3361 atgggcgcac gtagacttag ggagtggctg gcaatgccta taaaagatat ttcggaactt
     3421 aatctgcgat atgataccat ttcagccttc gtggctgatg aaaagatgct ggagaaggtg
     3481 aggagccgca tcggtgatat aggagatctg gagaggatca tctccaaggc ctccgctggc
     3541 aaactgctgc cacgcgaagc gcttcaactc tcacgaggcc tggatgccat agcggagatc
     3601 cgaaagctgc tattaaatag ttccaatccc gtattctcct cctgcataga gcagctggat
     3661 gcttgcgggg agctgtcaaa gagcatttcc acccgtatat tgcccgatac cgccgctcaa
     3721 ataggcaagg gagacgtgat agcgagggga gtc
//
LOCUS       DAOSIE010000136         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_21703_length_3727_cov_4.955065, whole genome shotgun sequence.
ACCESSION   DAOSIE010000136 DAOSIE010000000
VERSION     DAOSIE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21703_length_3727_cov_4.955065"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /gene="trmD"
                     /locus_tag="PLI69_04810"
     CDS             complement(<1..557)
                     /gene="trmD"
                     /locus_tag="PLI69_04810"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOG41843.1"
                     /translation="MRIDILTVVPELLESPLNHSIIKRAKEKGLVEIHLHNIRDYGKG
                     HYRQTDDYGFGGDAGMIMMIEPVFNLIEKLKGERDYDEIIYTAPDGEILDQKMVNSLS
                     IKENIIILCGHYKGIDHRIREHLITREISVGDYVLSGGELPAAIITDAVVRLIPGAIT
                     DETSALSDSFQDGLLAPPIYTRPAE"
     gene            645..3044
                     /locus_tag="PLI69_04815"
     CDS             645..3044
                     /locus_tag="PLI69_04815"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOG41844.1"
                     /translation="MSEDLKPIVPEQQEEALEQSVVNAEQVAEIPSDISQKEIVQPEK
                     AVEEAPKAKAPKKAPKAKAAEETPETEVAEDAPKAEVAEETPKAKAPKKATKAKAAEE
                     TPETEVAEDAPKAEAAEEAPKAKAPKKAPKAKAAEETPETEVAEDAPKAEVAEETPKA
                     KAPKKATKAKAAEETPETEVAEDAPKAEAAEATPKVKAAEETPKADTPEETPEVPDYS
                     EMGLKELLDLFQMLLDRGDMQLLYKHADSIKAIFYKTLRKEKIASGFIAPSEDVISDG
                     TESGEVVSINPFAEVERGFKELYTNYRTLRNAYAREQEQNKEQNLQKKLDIIEQIKAL
                     VERQSQEDMGQLFSEFGDLQAQWRATGQVPQARMRDLYESYSHNVEIFYDNVKIHKEL
                     RDLDFKRNLTAKEELCVKAEALAETEYDNIVAAFKALQKLHEEWKEIGPVGKEHRESI
                     WERFKAATAIINKKHQAYFDSLKQSQKENFEAKSALCEEVEKIVAMEISDSRTWNKMT
                     KAIEEIQAKWKAIGFASKKDNVRVYDRFRAACDQFFEAKKTYYAQLKEQMQENLKLKI
                     DLCEKAEAVQHSEEWKKTTDYLIDLQKQWKEIGPVSRKRTDQVWKRFRAACDVFFNNK
                     EKHFGKQESQYEANRQLKLALIEEIKAFELSDNKNDNFEALKAFQSRWNEIGFVPFRE
                     KNKLQDAFKKAIDAKFDEVRAQNDDYRPRYGKYSSDANLSKAERGLRTERDRLVQKYV
                     KKEQDIATWENNMGFFSKTKNAETYLAKMRKEIEVAREELAQLEEKIKAIDSQFEQRD
                     E"
     gene            3037..>3727
                     /locus_tag="PLI69_04820"
     CDS             3037..>3727
                     /locus_tag="PLI69_04820"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HOG41845.1"
                     /translation="MSKGENRSTIIGFALIGIILLLFSWYNTKQYEKQQKERYVADST
                     AAARALESARLDTTYHAVADEEAHIPGQELPLYQDSLLHFASVAEAEYYTLENDKIRL
                     TISSRGAQPSEVEIKNYYTYDSSALILIRDGKSTFEYEINTNQWINTGDLSFSLVSQS
                     ESNIILRLYFSTNSYIEAVYSLAEESYMVDYNLRFVNMDGILDRRTNQLRLHWNADMP
                     HLEKGYKNEKNY"
BASE COUNT         1194 a          780 c          885 g          868 t
ORIGIN      
        1 aactccgcag gtctggtgta gatcggcgga gccagtaatc catcttgaaa agagtcggac
       61 aaagccgagg tttcatcggt tattgctccg ggtattaatc tcacaacagc gtcagtgatg
      121 atggctgcag ggagttcacc tccgctgagc acataatctc ccacagagat ttcccgggta
      181 atcagatgct ccctgatacg gtgatcaatc cctttataat gaccacagag aatgataata
      241 ttctctttaa tagagaggga attgaccatt ttctggtcga gtatttcacc gtcgggtgct
      301 gtatagatta tttcatcata gtctcgttct cctttaagtt tttctatgag gttgaagacc
      361 ggttcaatca tcattatcat tcccgcatcg cctccaaatc cataatcatc ggtctggcga
      421 tagtgtccct ttccataatc tcgtatgttg tggagatgga tttcgacaag tcctttttcc
      481 ttcgctcttt tgattatgga gtggtttagc ggactttcaa gcagttccgg tacgaccgta
      541 aggatatcta ttctcatttt ttgtgctctt gtagtgcaaa agtagcattt attccacaaa
      601 ttccgttata tttgcaggat ataataatta tataattcaa ttacatgagt gaagatctga
      661 aaccaattgt tcctgagcaa caagaagaag ctctagagca atctgttgta aacgctgaac
      721 aggtcgcgga aattcccagc gacatatccc agaaagaaat tgtacaaccg gaaaaagcag
      781 ttgaagaggc tcccaaagca aaagcaccta aaaaggcccc caaggcaaaa gctgctgaag
      841 agactcctga aacagaagta gctgaagatg ctcccaaggc agaggtcgct gaagaaactc
      901 caaaagcaaa agcacctaaa aaggctacta aggcaaaagc tgctgaagag actcctgaaa
      961 cagaagtagc tgaagatgct cctaaggcag aggccgcaga agaggctccc aaagcaaaag
     1021 cacctaaaaa ggcccccaag gcaaaagctg ctgaagagac tcctgaaaca gaagtagctg
     1081 aagatgctcc caaggcagag gtcgctgaag aaactccaaa agcaaaagca cctaaaaagg
     1141 ctactaaggc aaaagctgct gaagagactc ctgaaacaga agtagctgaa gatgctccta
     1201 aggcagaggc cgctgaagcg actccaaaag taaaagccgc tgaggagacc ccaaaagcag
     1261 atactcctga agagaccccg gaggtgcccg attattcgga gatgggtctg aaagagcttt
     1321 tggatctatt ccagatgcta cttgacaggg gagatatgca gctgctttac aaacatgccg
     1381 attccatcaa ggccatattt tacaagactc tccgcaagga gaagattgca agtggcttca
     1441 ttgctccttc cgaggatgtg ataagtgatg gcacagagag tggcgaggtt gtttccatca
     1501 atccctttgc tgaggttgag cgtggtttta aagaacttta cactaattat cgcactttgc
     1561 gcaacgccta tgcgcgtgag caggagcaaa ataaggagca gaatcttcag aaaaaactgg
     1621 atatcattga gcagataaaa gctcttgtcg agaggcagag tcaggaagat atgggtcagc
     1681 ttttcagtga gtttggagat ctccaggcac aatggcgcgc aaccggtcag gtgccacagg
     1741 cacgtatgag agacctttat gagtcctaca gccacaatgt agagatattt tacgataatg
     1801 taaaaattca taaggagctc agggatctcg atttcaagcg caatcttact gcaaaagagg
     1861 agctttgtgt taaggctgaa gctcttgcag agactgagta tgacaatatt gtagcagctt
     1921 ttaaggcgtt acagaagctc catgaggagt ggaaagagat aggtcctgtc ggaaaagagc
     1981 atagggagag catctgggag aggtttaagg cggccactgc gataatcaat aagaaacatc
     2041 aggcatattt cgactctcta aaacaaagtc agaaggaaaa tttcgaggca aaaagcgccc
     2101 tttgtgagga agtggagaag atcgttgcaa tggaaatctc cgactcaaga acctggaaca
     2161 agatgaccaa agccatcgaa gaaatccagg ccaaatggaa ggccataggt tttgcctcca
     2221 aaaaggacaa cgtcagggta tatgaccgct tccgcgccgc ctgtgaccag ttctttgaag
     2281 caaagaaaac atattacgca cagctcaaag aacagatgca ggagaatctt aagttgaaaa
     2341 tagatctttg tgaaaaggct gaggccgttc aacatagcga agagtggaag aagactaccg
     2401 attatttaat cgaccttcag aaacagtgga aagagatcgg tcccgtctct cgtaaacgca
     2461 ccgaccaggt atggaaacgt tttcgcgctg cctgcgatgt gtttttcaat aacaaggaga
     2521 agcattttgg caaacaggaa tcacagtacg aagccaaccg tcagcttaag cttgcactaa
     2581 ttgaagaaat caaggcattt gaactatcag ataacaaaaa cgataatttt gaggcattga
     2641 aggcattcca gagcagatgg aatgagatcg gctttgttcc tttcagggaa aaaaataaac
     2701 tccaggatgc attcaagaag gcgattgacg ctaagtttga cgaggttagg gctcagaatg
     2761 acgactatcg tccgagatat ggaaaatact cttccgatgc caatttaagc aaggcagaga
     2821 gaggtctccg taccgaaaga gaccgcctcg tacagaagta cgtaaagaag gaacaagaca
     2881 ttgctacctg ggaaaacaat atgggctttt tctccaagac taaaaatgcc gagacctatc
     2941 ttgccaagat gaggaaagag attgaggtcg cccgtgagga gcttgcacag ctcgaggaga
     3001 agatcaaggc aattgattca caatttgagc agagagatga gtaaaggaga aaacagaagc
     3061 acaatcatag gatttgccct tatcggcata atattgttac ttttcagctg gtacaatacc
     3121 aaacagtatg agaagcagca gaaagagaga tacgtagcag attcgactgc ggcagcccgc
     3181 gcacttgaaa gtgcccgtct ggataccaca tatcacgcag tagcagatga agaagcgcat
     3241 atcccgggtc aggaactgcc gttgtatcag gattccttac tacactttgc cagtgtcgcg
     3301 gaggctgagt attatactct cgagaatgac aaaatcaggc tgaccatttc ctccagaggt
     3361 gcacaaccct cagaagttga aattaagaat tattacactt atgacagctc tgctctgata
     3421 ttgatcaggg atggaaagag cacattcgag tatgagatca acaccaatca gtggatcaac
     3481 accggtgatc tatcgttttc ccttgtttca cagagtgaaa gtaatataat actccgtctc
     3541 tatttttcca ccaactccta cattgaggca gtttattctc tcgctgaaga gagttatatg
     3601 gtggactaca atctcagatt tgtgaatatg gacggcatac ttgacagacg tacaaatcaa
     3661 ctacgtctcc attggaatgc cgatatgcca catcttgaaa agggctacaa aaatgagaag
     3721 aattact
//
LOCUS       DAOSIE010000137         3715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_21792_length_3715_cov_3.409016, whole genome shotgun sequence.
ACCESSION   DAOSIE010000137 DAOSIE010000000
VERSION     DAOSIE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21792_length_3715_cov_3.409016"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLI69_04825"
     CDS             <1..703
                     /locus_tag="PLI69_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG41846.1"
                     /translation="YFSPQGNSNWIKRIQMLKSRYNQENEVEKFVANKAPYRFIQALN
                     NITQEDDIITVDIGQNQMWAAQTVKLKRGQKFITSGGLAPMGFSLPVAIGMAFASPDK
                     TIYAITGDGGLHISIQSLLLIPQYNLNIKIIVLNNSALGMITQFQSLYFDGRMKGTTA
                     QSGFINPDYEGIAKAYGLNYVARNEESLSTIKEDLAIRNIFYEYQIEDLTTVSPKLEY
                     NRPINQPSPQINENE"
     gene            696..1667
                     /locus_tag="PLI69_04830"
     CDS             696..1667
                     /locus_tag="PLI69_04830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG41847.1"
                     /translation="MNKEILKKISVLIPCYNEEENVVQIASAVSNEIKSNLPDYDYEI
                     VFIDNNSKDSTRPLIREICKRNPKVKAIFNAKNFGPVNSPYYGLLQTGGDCTILLCCD
                     FQDPVEMIPLLVDEWEKGAKIVSCIKTKSKENRVMRFIRTCYYKIIKKMSSIEQIEHF
                     TGFGLYDKSFIDVLRKLEDPTPFLRGIVAELGYKRVSIEYTQAKRRAGKPKNSFMDLF
                     EFAMLSFTSYTKVGMHLVTITGFILAILSILVGLVYLVLKLIYWDRFSAGIMPMLIGL
                     FFLHAVELVFIGLVGEYVMSVNTRVMKRPLVIEEERINFESEKTPAE"
     gene            1772..2980
                     /locus_tag="PLI69_04835"
     CDS             1772..2980
                     /locus_tag="PLI69_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOG41848.1"
                     /translation="MFDINKFISKYITKRDKSLFLNDIESNRQQLEEVIRDKRVCVIG
                     GAGSIGSSFIKALLPFRPSSLVVIDLSENSLVELTRDLRSTKGMFVPDDYRTYTLDFA
                     SPLFEQMFIREKGFDIVANFSAHKHVRSERDTLSVQALITNNVFKAKVLMELLSKYPP
                     KHFFCVSTDKAANPVNIMGASKLLMEKLVMSYRDRFPVTTARFANVAFSNGSLPDSWI
                     SRIMKNQPLVAPSDVKRYFVSPQESGEICMLACILGKSGEIFFPKLKEEQMITFSSIC
                     DKFVKELGYIKKEFDNESEARLYASQMKEGDKEYPVFYFKSDTTGEKDFEEFFIPGEE
                     VDMESFSSLGVIKVEDCCSVDEISDFLSKLELLFDFKECNKADIINTLCGFLPTFEHE
                     EKGKNLDQKM"
     gene            2990..>3715
                     /locus_tag="PLI69_04840"
     CDS             2990..>3715
                     /locus_tag="PLI69_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG41849.1"
                     /translation="MYSKIASYIRELYNSQDFIPLSAPVFIGNEKKYLEECVDSTFVS
                     GVGKFVDRFEKMVADYTGAGKAVVCVNGTNALHMAMMLAGVKREDEVITQALTFVATC
                     NSISYIGAYPIFIDVDRDTMGLSPTAVDNWLSANAQLRGDECFNKSTGRRIRCCVPMH
                     TFGHPVHLDELMDVCNKWHLELVEDAAESIGSFYKGKHTGTFGRIGIISFNGNKTITT
                     GGGGMLLFNDEALAARAKHLTKQA"
BASE COUNT         1178 a          695 c          796 g         1046 t
ORIGIN      
        1 atatttttct ccacaaggaa actctaattg gattaaacga atccaaatgc ttaaatccag
       61 gtacaatcaa gaaaatgagg tagaaaagtt tgtagcgaac aaagcacctt acagatttat
      121 tcaggcactg aacaatatca ctcaggaaga tgatataata acagttgaca ttggacaaaa
      181 tcagatgtgg gcggctcaaa ctgtcaaatt gaagagagga cagaaattca tcaccagcgg
      241 aggcctggct ccaatgggat tttcacttcc tgtggctata ggaatggcat tcgccagtcc
      301 tgataaaacc atttatgcaa taacaggaga tggaggactc catatctcta tccagtcgtt
      361 gttgctcatt ccccaatata atctaaatat taagataatt gttctcaaca atagtgcgct
      421 gggtatgata actcaatttc aatcattata ttttgatggc agaatgaaag gcacaactgc
      481 acagagcggc ttcataaacc ctgactatga agggattgct aaagcttatg gcttgaatta
      541 cgtggcaaga aatgaagaat ctttgagtac cataaaggaa gatttggcaa ttcgtaatat
      601 tttttacgaa taccaaatag aggacctgac gactgtctcc ccaaaattgg aatataacag
      661 acccatcaat caaccttctc cacagattaa tgaaaatgaa taaagagatt ttgaaaaaaa
      721 taagtgtatt gattccttgc tataacgagg aagagaatgt ggtacagata gcgtcggctg
      781 tatcgaatga aatcaagagc aatctgcccg attacgatta cgaaatagtc tttattgaca
      841 ataactctaa ggatagtact cgcccgctaa tccgggaaat ttgtaagaga aatcccaagg
      901 ttaaggctat tttcaatgct aaaaacttcg gtccggtcaa cagtccttat tatggattgc
      961 tacaaactgg tggagattgc acaattttgt tatgttgtga ttttcaggat cctgtggaaa
     1021 tgatacctct tttggtagat gagtgggaaa agggagcaaa aatagtctct tgcatcaaaa
     1081 caaagagcaa ggaaaataga gtaatgcgtt ttataagaac atgttactat aagatcatca
     1141 aaaagatgtc ctcaatagaa cagattgagc attttaccgg cttcggacta tatgacaagt
     1201 catttatcga tgttttgaga aaattggagg atccaactcc tttcttaaga ggaattgttg
     1261 ctgaattggg ttataaaaga gtttcgattg aatatactca agcaaaaaga agagccggta
     1321 aacccaaaaa cagttttatg gatttgtttg agtttgccat gctgagtttc acctcatata
     1381 cgaaggttgg aatgcatctt gtgacaataa caggctttat actagccata ttgtcgatat
     1441 tggtaggttt ggtatactta gtactcaaat taatctattg ggaccgattt tctgctggta
     1501 taatgcctat gttgatagga ttatttttcc ttcatgccgt ggagcttgtt tttatcggtc
     1561 ttgtgggcga atatgttatg agtgtaaata cacgagtaat gaaaagacca ttggtaatag
     1621 aggaagagag gataaatttt gaatctgaga agactcccgc cgaatgaata agccgtagct
     1681 aatgacataa ataagcttca cgaaagtcgt caatgacttg caagaaatta ttactaactt
     1741 tgtaggttaa taacaaaatg cgcaaatcct aatgtttgat atcaataaat tcatttccaa
     1801 atatataacc aaaagggata aaagtctttt tctaaatgac atagaatcca accggcagca
     1861 gctcgaagaa gtaattcgtg acaagagggt atgtgtaata ggaggtgccg gcagcatagg
     1921 atcctcattc atcaaagccc tgcttccgtt cagaccatct tcccttgtag tcattgactt
     1981 gagcgaaaac agcctggtag aactaaccag ggacctcaga tctaccaagg gtatgtttgt
     2041 gccggatgat taccgcacat acacgcttga cttcgccagc cctttatttg agcaaatgtt
     2101 tatcagagaa aaagggtttg acattgttgc aaatttttct gctcacaaac atgtccgtag
     2161 cgaacgcgac actctctccg tacaggcact tattactaac aatgttttca aggcaaaggt
     2221 tctgatggag ctcttgtcca aatacccgcc taaacacttt ttctgtgtct ccacagacaa
     2281 agcagcaaat ccggttaata ttatgggagc cagcaaactc ctgatggaaa agttggtgat
     2341 gtcttaccgt gaccgtttcc ccgtaactac tgccagattt gcaaatgttg cattttccaa
     2401 tggctccctg cctgacagct ggattagcag aataatgaaa aatcagcctt tggtggctcc
     2461 gtcagacgtc aaaagatact ttgtttcacc tcaggaaagt ggagaaatat gtatgcttgc
     2521 ctgcattctc ggcaagagcg gagagatatt ttttccaaaa ctcaaagaag agcagatgat
     2581 tactttttct tcaatctgcg ataaatttgt aaaggagtta ggctatatca aaaaggaatt
     2641 tgacaatgaa agtgaagcac gtttgtatgc ttcgcagatg aaagaaggtg ataaagaata
     2701 tcctgtattc tattttaaga gcgacactac cggagaaaag gatttcgagg agtttttcat
     2761 tcccggagaa gaggtggata tggagagttt ctcatcgctt ggggtgatta aagttgaaga
     2821 ttgctgctca gtagatgaaa tctccgattt tctttcaaaa cttgaattgc tttttgattt
     2881 caaggagtgt aataaagcag atattatcaa taccttatgc ggttttctgc ccactttcga
     2941 acatgaggaa aagggcaaaa accttgacca gaaaatgtaa ttaacaacga tgtatagcaa
     3001 gattgcgtca tatataagag aactctataa ttcacaggat tttatccctc tcagcgcacc
     3061 ggtatttatc ggtaatgaga agaaatacct ggaagagtgt gttgattcca cttttgtctc
     3121 cggcgtagga aagtttgttg accgttttga aaagatggtt gcagactaca cgggagcagg
     3181 gaaagctgta gtttgtgtta atggcactaa tgctcttcat atggctatga tgctagcagg
     3241 cgtgaagcgc gaggatgaag tgatcactca ggctctcacc tttgttgcaa catgtaactc
     3301 gattagttat attggagctt atcccatctt cattgatgtt gaccgcgaca caatgggact
     3361 ctccccaaca gctgtagata attggctgag tgccaacgca caactacggg gagatgagtg
     3421 cttcaacaaa agcaccggcc gtcgaatccg ttgttgcgtg ccgatgcaca cattcggtca
     3481 tcccgtccac ctcgatgaac ttatggatgt ttgcaacaaa tggcatttgg aactggttga
     3541 agatgccgcc gaaagcattg gctcatttta caaaggaaaa cataccggta ctttcggccg
     3601 cataggcatc atttctttca acggaaataa aaccattacc accggaggtg gaggaatgct
     3661 ccttttcaat gatgaagcac ttgccgcccg agcaaaacat ctcactaagc aggca
//
LOCUS       DAOSIE010000138         3690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_21999_length_3690_cov_3.158184, whole genome shotgun sequence.
ACCESSION   DAOSIE010000138 DAOSIE010000000
VERSION     DAOSIE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21999_length_3690_cov_3.158184"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PLI69_04845"
     CDS             complement(<1..818)
                     /locus_tag="PLI69_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG41850.1"
                     /translation="MVIYQLLLRAFGKRKSVPGGSYSINGSGKFDDVTDEVLKKIKKL
                     SVSAIWYTGVISHATKTHFNGIKDCNGSIVKGEAGSPYAIRNYFDVSPALANNHDNRM
                     AEFEALIHRTHKAGMKVIIDFVPNHVFRQYDTYFTKDNFYLLNGALVLPESLNSDYVE
                     NPAKATGNDVFHTSPSIHDWYDTVKLNYDNHGTWVKMLEILKFWVSKGVDGFRCDMVE
                     LVPAEFFGWAFSEIKYIYPNTFFIAEVYGKENYRHYHEIGGFDLLYDKSGFYDT"
     gene            complement(830..2062)
                     /locus_tag="PLI69_04850"
     CDS             complement(830..2062)
                     /locus_tag="PLI69_04850"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOG41851.1"
                     /translation="MKESIIILTGGGPAPGINTVIGSIAKAFLARGYRVLGLHGGYKG
                     LFTPDPSYTEIDFVMADSIFNRGGSHLMMSRYKPTEKDYEGNFNHKLFQDNNIKLLVT
                     IGGDDTASTANRITKFLQARNYKISNIHVPKTIDNDLPLPLNTPTFGYHSAKAEGTRI
                     ATTVYEDARTSGNWFIVAAMGRSAGHLAIGIGSSCHYPMIIIPEMFNKSKITVDKIIK
                     LSISSIIKRKLMGIPYGGIMLSEGVFHLLDEDELKATGVRFTYDEHGHPELGKISKAQ
                     LFNDILEIELKKLGMNIKMRPIEIGYDIRCQAPISYDLLYCTQLGCGVYELYTRGESG
                     CMVYVDTIGRIKPLYLKDFQDPKTGKIPPRLVDIEGDQAQGIYKHIVHYITPNDYEAA
                     KEYVSNPEEYDFHKILNW"
     gene            2153..>3690
                     /gene="ligA"
                     /locus_tag="PLI69_04855"
     CDS             2153..>3690
                     /gene="ligA"
                     /locus_tag="PLI69_04855"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOG41852.1"
                     /translation="MAKISKSVAKERIEQLRRELEEHNRNYYVLSTPVISDFEYDMLM
                     LELQGLEARFPEFASEESPTRKVGSDLTEISEGFAQVEHRYPMLSLSNTYNEGELIDF
                     HQRVENVAQAPFSYSCELKIDGTAICLIYREGKLAQAITRGDGIKGDDVSRNIVSIDS
                     IPHILHGKNIPAEFEIRGEIFMPYEAFDRLNAERLEAGEAPFANPRNAASRSLKLLDP
                     SKVKERGLDCILYHLLGENLPFSTHSEAIEAAASWGLHVSEHSIVARNIEEVTGYIEE
                     WEERRKRLPYATDGIVIKVNRLDLQRQLGFTAKSPRWATAFKFKPEEALTKVLSIDYQ
                     VGRTGAITPVANLEPVPLSGTTVKRASLHNFDQMELLDIRVGDLVYVEKGGEIIPKIT
                     GVEFSKRHADVEKPVFPTVCPDCGTPLERDPEQARHYCPNSEGCPTQIKGRFIHFVSR
                     KAMDILAGEATISLFYDKGLIRTLPDLYRLKAEDILGLEGWQQKSVDNLLSSIERSKQ
                     VPFD"
BASE COUNT          949 a          881 c          900 g          960 t
ORIGIN      
        1 aaagtatcgt agaagcccga tttgtcatac aaaaggtcaa agccgcctat ctcatgatag
       61 tgtctgtaat tctccttacc gtaaacctct gcaatgaaaa aggtattggg atagatatac
      121 tttatctcac tgaaagccca gccgaaaaac tccgcaggca ccaattccac catatcgcag
      181 cggaaaccat ccaccccttt cgacacccaa aatttgagga tttccagcat tttaacccag
      241 gtgccatggt tgtcgtagtt gagcttgaca gtatcatacc agtcatggat tgaaggagag
      301 gtgtggaaca catcattgcc ggtagccttg gcgggatttt ctacataatc ggagttgaga
      361 gactccggca gcaccaacgc tccgttcagc aggtagaaat tgtccttcgt aaaataagta
      421 tcatactgtc gaaacacatg atttggcaca aagtctatga taaccttcat ccccgccttg
      481 tgagtgcgat ggattagcgc ttcaaactcc gccatacggt tgtcgtggtt attggcgaga
      541 gccggactca cgtcaaaata attgcgaatg gcataagggg aacctgcctc tcccttcacg
      601 atcgagccgt tgcaatcctt tataccattg aagtgcgtct tggttgcatg agatattact
      661 ccggtatacc agattgcgct gaccgagagc tttttgattt ttttaagtac ttcatcggtg
      721 acgtcgtcaa acttaccgct gccattgata ctgtaagagc ctccgggtac gctcttgcgt
      781 ttgccgaaag ctcttagtaa cagctggtat atgaccacct tcccttgcac taccagttta
      841 gtatcttgtg aaagtcgtac tcttcagggt tgctgacgta ctctttggca gcctcgtagt
      901 cgttaggggt aatgtagtgt actatgtgtt tatagatgcc ctgtgcctga tcaccctcga
      961 tgtccaccag acgcgggggg atcttacctg tcttgggatc ctgaaagtct ttgaggtaaa
     1021 ggggcttgat acgaccgata gtatccacgt agaccataca tcccgactct ccgcgtgtgt
     1081 agagttcata aactccgcaa ccgagctggg tacaatagag caggtcgtaa gagatgggag
     1141 cctggcatct gatgtcataa ccgatctcaa tgggacgcat ctttatattc attccgagct
     1201 ttttgagttc aatctcgaga atgtcgttaa agagctgagc ctttgatatc ttgccaagct
     1261 cgggatgtcc atgctcgtca taagtaaatc tcacgcccgt agccttcagc tcatcctcat
     1321 ccagaagatg gaagactcct tcggagagca ttattcctcc gtagggaata cccatcaatt
     1381 ttctcttgat gatggaagag atggagagtt ttatgatctt gtctacggta attttggatt
     1441 tgttgaacat ctcggggatg ataatcatcg ggtagtggca ggaagagccg atacctatgg
     1501 ccagatggcc tgcagaacgc cccatcgcgg ccacgataaa ccagttgccc gaggtcctcg
     1561 catcctcata taccgtggtg gcgatacgtg taccctcagc ttttgctgag tgataaccga
     1621 atgttggagt attgagtgga agaggaaggt cgttgtcaat agtcttggga acgtgaatat
     1681 tggagatttt gtagttcctt gcctgaagga atttggtaat cctgttggca gtagaagcag
     1741 tatcgtcacc gccaattgtc acaagcagct tgatattgtt gtcctgaaaa agtttatggt
     1801 taaaatttcc ttcatagtcc ttttcggtgg gcttgtatcg actcatcata aggtgcgagc
     1861 cgcctctgtt gaagatagag tcggccatca caaaatctat ttcagtatat gaagggtcgg
     1921 gagtgaaaag tcctttatat ccaccatgga ggccgagtac cctatatcct ctcgctaaaa
     1981 aagctttcgc tatgctacct ataaccgtgt ttatgccggg agcgggtcct cctcctgtta
     2041 aaataatgat agattctttc ataaaatgtt cgtatttgat tttccggttg caaatgtact
     2101 actttttgat aaaattgtac taattttgta agttattagc aaattgcagg atatggctaa
     2161 aatttctaaa tcggtagcaa aggagagaat agagcaattg cggcgtgagc tcgaagagca
     2221 caaccgcaac tattacgtgc tctctactcc cgtcatatcc gatttcgaat atgatatgct
     2281 gatgctggag ctccagggac tggaggccag attcccagaa tttgcgagcg aagagtcccc
     2341 tacccgcaag gtgggcagcg accttacgga aataagcgag ggatttgctc aggttgagca
     2401 ccgttatccc atgctttcac tcagcaacac atataatgaa ggtgagctta tcgattttca
     2461 ccaaagggtg gagaatgtgg cacaagctcc cttctcctac tcttgcgagc tgaaaattga
     2521 cggtaccgcc atctgtctca tctacaggga aggcaaactg gcacaagcca ttacacgggg
     2581 agatggcatc aaaggagatg atgtctcacg caatattgtc agcatagact ccatccccca
     2641 cattctccat ggtaaaaaca ttcctgccga atttgaaatc aggggtgaga tatttatgcc
     2701 ttatgaggct ttcgaccgcc tgaatgcaga gaggttggaa gcgggagaag ccccttttgc
     2761 caacccccgg aatgccgcct cacgctccct gaaacttctc gacccttcca aggttaagga
     2821 acgcggcctc gactgcatcc tctatcacct actcggagag aaccttcctt tcagcacgca
     2881 tagcgaagct attgaggcag cagcatcctg gggattgcat gtatcggaac atagcatcgt
     2941 agccaggaat attgaggaag ttaccggata tatcgaagag tgggaagaac gcaggaaaag
     3001 actcccttac gctaccgacg gcatagtgat caaagtgaac cggctcgacc ttcagcgtca
     3061 gctgggcttt acggccaaat ctccgcgttg ggccaccgca tttaaattta aaccggaaga
     3121 ggctcttacc aaggtgctct ccatagatta tcaggtgggc aggacgggtg cgatcactcc
     3181 ggttgccaat cttgagcctg tgcctctctc aggtaccacc gtaaaacgcg cctcactcca
     3241 caatttcgac cagatggagc tgctcgatat acgcgtaggc gatctggtat atgtggaaaa
     3301 gggcggcgag attattccca agataaccgg tgtggaattc tctaagcgcc atgcggatgt
     3361 ggaaaagccg gtttttccta cggtatgtcc cgattgtggc actcccctgg agagagaccc
     3421 ggagcaggca cggcactact gccccaacag tgagggctgc cccacgcaga tcaaggggcg
     3481 cttcatccat ttcgtctccc gaaaggctat ggatatactt gccggagagg cgaccatttc
     3541 gctcttctac gacaaaggat tgatacgcac gcttcccgat ctgtaccgac tgaaggcgga
     3601 agatatattg ggactcgaag ggtggcagca gaagtcggtt gacaatctgc tttccagcat
     3661 agagcgatcc aaacaggtgc catttgatag
//
LOCUS       DAOSIE010000139         3677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_22118_length_3677_cov_3.403644, whole genome shotgun sequence.
ACCESSION   DAOSIE010000139 DAOSIE010000000
VERSION     DAOSIE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22118_length_3677_cov_3.403644"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2545
                     /locus_tag="PLI69_04860"
     CDS             <1..2545
                     /locus_tag="PLI69_04860"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG41853.1"
                     /translation="NLPGKMMINYFESIGASFGANINASTGVEQTMYMLNNIPTTREG
                     IIDTALLIMHDYSAFVTNDPVEIDKERGVIIEEWRTRRTAEWRSTEKAWEHLYKGSKY
                     ATCNIIGTKENLETFPASELQDFYHTWYRPDLQAIVVVGDIDPEVIEAKLRELFKDIP
                     AHENPKPKEEILLPENEEPIVGIITDPETRVSQIQFAVKYPLLTPRMVRRFGAGFMND
                     LAVDLVNAVFSERFDDIRKKADAPFFAGGAAFTNLILTSDALMASAAFRDGEALTAFE
                     AALTEIEKAKRFGFTESELERAKTNMIANAERATSNAESRKNGEYVRDYMSDFFQGWP
                     FMTPAYKEEQIKGYLPYLSADVINAFLKTISYDKNVVVLYLAPEREGLVHPVEQDFIT
                     IIDKVKNAEMESGIQEEELGELVDSALLLGSKVKEEKEGLFNSTVWRLENGINVVVRP
                     SDYKKEEVTFRLQVPGGKSLIAESELPSVDDNMLVLYGNLSGVSHFPSTILSKMLTGK
                     IVSESPFIGDEFHGITGQSAPKDFETLMQLVYLQVTEPRFVAEEIAPAFAQMNAIVPN
                     LSKQPNYIIGDAYMKTAYGNNPRKQLISMDMVSKMSVASLEQSYKTLFSNMAGASVYI
                     TGNVDLETIKPLVEKYIGSLPAIGEGSTYIDHNIQVVPGKVENVFNAPMTTPKSTVLL
                     IYSGDVKYDLENVLTMQAFRYVLDLVYTETIREEEGATYGVGTQGSVSAIPQNRGEML
                     IQFDTDPARAEDMIRLAIKGVEDLANNGPSEEQMVKVMENFLKNVSESRINNSYWSNN
                     LRQFDLTGVDLDTEREAVINSLTSEKVKNFVADFIGQGNFVKIVMNPEK"
     gene            2642..3046
                     /locus_tag="PLI69_04865"
     CDS             2642..3046
                     /locus_tag="PLI69_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41854.1"
                     /translation="MDLRLFLRFLSVALAVVLTLPYITYGQDLAENRILGIWMDEKGE
                     TRSEFYRNDDGTFGAKVLWHVNSKKQDRVNLVFIKDMHYNPSDSTWRTSYMYSPDHRL
                     VARGRLWIKDGILYVNGRKFGISATRRFFRIE"
     gene            complement(3084..>3677)
                     /locus_tag="PLI69_04870"
     CDS             complement(3084..>3677)
                     /locus_tag="PLI69_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOG41855.1"
                     /translation="ALRVELCNDLSVGGVTPAYDEIEQARTQLNIGLNVLIRPRGGDF
                     NYSAAEISRILRDIEFCGSLLTEEGKVVDGVVIGALSRDRSIDQGACREMFAAARQYA
                     LSITFHRAIDRSRDIFKALERVIDLGAERVLTSGGAESAFEGRETIARMVKMSIGSGT
                     LIMAGAGITPENASQIIAETSVPEIHGSRLSLLDGFV"
BASE COUNT         1054 a          801 c          866 g          956 t
ORIGIN      
        1 aaatctccca ggtaagatga tgatcaatta ttttgagagc atcggagcca gttttggtgc
       61 taatatcaat gcatccacag gagttgagca gacaatgtat atgctcaaca acatacccac
      121 tacgagggaa ggcattatcg acacggcgct tctgataatg cacgactact ctgcttttgt
      181 gacaaatgat cccgtcgaaa tagataagga gagaggtgtc atcatagagg agtggcgtac
      241 acgccgcaca gccgagtgga gaagtaccga gaaggcttgg gagcatctct acaaaggctc
      301 gaaatatgcc acctgtaata tcataggtac taaggaaaac cttgagacct tccctgcaag
      361 tgaacttcag gatttctatc acacatggta tcgtccggac cttcaggcaa tagttgtggt
      421 tggtgacatc gatccggaag tgattgaggc caaattgagg gaacttttca aggatatccc
      481 cgcccatgag aatcccaaac ccaaggaaga gattcttctt cctgaaaatg aagaacccat
      541 agtaggcatc atcaccgatc cagaaacaag agtgagccag atacagtttg cagtaaaata
      601 tccccttttg actccgagga tggtacgccg gtttggtgca ggctttatga atgaccttgc
      661 cgtagatttg gtaaatgctg ttttttccga gagatttgat gatattagaa agaaggccga
      721 tgctccattc tttgcaggtg gcgctgcttt tacgaacctg attctcactt ctgatgcgct
      781 tatggcaagc gcagcattta gggacggaga ggctttaaca gcatttgagg cagctttgac
      841 tgaaatagaa aaggcaaaaa ggttcggttt tacagaaagt gaactcgaga gggccaagac
      901 caatatgatt gcaaatgctg aaagggctac atccaatgcc gaaagtcgta agaacggtga
      961 atatgtacgg gattatatgt ctgacttctt ccagggctgg cctttcatga ctcccgcata
     1021 caaagaagag cagataaaag gttatcttcc ttacctttcc gctgatgtaa tcaatgcatt
     1081 ccttaagact atttcttatg ataagaatgt agtggttctt taccttgctc ctgaacgaga
     1141 aggccttgta catcctgtag agcaagattt cataactata attgataagg tgaaaaatgc
     1201 tgaaatggaa tcgggaattc aggaagagga gttgggagaa ctggtagatt cagcgcttct
     1261 tttaggcagt aaagtgaaag aggagaaaga gggtcttttc aactccactg tatggaggct
     1321 tgagaatggt atcaatgtcg ttgtaagacc ttcagactac aaaaaggaag aagtaacatt
     1381 ccgtctacag gtgccaggtg gaaaatcttt gattgcagag tcggaacttc cttcggttga
     1441 tgacaacatg ttggttttat atggcaattt gtcaggcgtc tctcattttc cctcaactat
     1501 tttgagtaag atgcttaccg gtaaaatagt ttccgaatca cccttcatcg gtgatgaatt
     1561 ccacgggata accggtcaaa gtgcaccaaa ggatttcgag acactcatgc aattggtcta
     1621 tctgcaggtg actgagccaa ggtttgttgc agaagagatt gcccccgcat ttgcccagat
     1681 gaatgccata gtgcctaatc tttccaaaca gccaaattat attatcgggg atgcatacat
     1741 gaagactgct tacggaaata accctcgcaa acagttgatc tcaatggaca tggtaagcaa
     1801 aatgtccgtg gcatcacttg aacaatccta caagacccta ttctccaata tggccggtgc
     1861 cagcgtatat atcacaggca atgtcgacct tgagaccatc aagcctttgg tagagaaata
     1921 tatcggttct ctccccgcta tcggtgaggg aagcacatat atcgatcaca atatccaagt
     1981 tgtgccaggt aaagttgaga atgttttcaa tgcccctatg actactccaa agagtaccgt
     2041 attgctgata tatagcggtg atgtaaaata tgatcttgaa aacgtgctta caatgcaggc
     2101 cttccgttat gttctcgatc tggtttatac cgaaactatc agggaagagg agggtgcaac
     2161 ttatggtgtg ggcacacagg gttccgtttc agcaatacct caaaacaggg gcgagatgct
     2221 cattcagttt gatacggatc ccgccagggc tgaagatatg atcagattgg caattaaagg
     2281 tgttgaggat ctagcaaata atggccccag tgaagagcag atggtaaaag taatggagaa
     2341 tttcctcaaa aatgtatccg aatcccgtat caacaacagt tattggtcaa acaacctgcg
     2401 tcagttcgat cttacaggtg tggacctaga tactgagcgc gaagcagtaa tcaattccct
     2461 cacctctgaa aaggtgaaga attttgttgc ggacttcatc ggccagggca actttgtcaa
     2521 gattgtgatg aacccggaaa aatagctgtt ggctgttggc cgttggcttt taagaattag
     2581 ccaatggcca atggccaaag gctaatggct aaaggctaat ggctagcagg gtagaggtaa
     2641 tatggatttg cggcttttct taaggttctt atcggtggca ttggcggtag tactgactct
     2701 gccctatatc acttatggtc aggatctggc agaaaatagg atacttggta tatggatgga
     2761 tgaaaagggc gaaacaaggt ctgagtttta tcgcaatgat gacggaactt tcggtgcgaa
     2821 agtcctctgg catgtgaatt ccaaaaagca ggaccgcgtc aatcttgtct tcattaagga
     2881 tatgcactac aacccttccg acagcacctg gagaacatct tatatgtact cacccgacca
     2941 tcgattggta gcaaggggta gactttggat taaggatggt attctttatg tcaatggtcg
     3001 caaattcggt atctctgcca cccgccgctt ttttcgcata gagtagttgt attcgcggga
     3061 cttccgatag gacagatatt ttttcagaca aaaccgtcca acaaacttag acgggagccg
     3121 tgaatttccg gaacggaggt ttctgcgata atttgactgg cattttcggg agtaatcccg
     3181 gctcctgcca ttatcaatgt accgctgcct atactcattt ttaccatacg ggcaatagtc
     3241 tcacgccctt caaatgccga ctctgctcct cccgaagtta gcacgcgctc tgccccaaga
     3301 tcaatgactc tctccaaagc cttgaatata tctctagaac gatcaatcgc acgatgaaat
     3361 gtaatcgaaa gggcgtattg acgcgcggca gcgaacattt cccggcaagc accctgatct
     3421 atggagcgat cacgcgacag agcaccgatc accacaccat ccaccacctt tccttcctcc
     3481 gtaagcagag aaccgcagaa ctcaatgtct ctcagaatac gggaaatctc ggcagccgaa
     3541 tagttaaaat cccctccacg cggtcttata agcacattga ggccgatatt caactgcgta
     3601 cgcgcctgct ctatctcatc ataagcaggc gttactcctc ctacggagag atcattgcag
     3661 agttctactc tgagagc
//
LOCUS       DAOSIE010000140         3634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_22438_length_3634_cov_3.224923, whole genome shotgun sequence.
ACCESSION   DAOSIE010000140 DAOSIE010000000
VERSION     DAOSIE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22438_length_3634_cov_3.224923"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLI69_04875"
     CDS             <1..343
                     /locus_tag="PLI69_04875"
                     /inference="COORDINATES: protein motif:HMM:NF021546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOG41856.1"
                     /translation="DSILTGNKQSVQIMIFSSKYAEIADMINTKLNRGVTALNSIGWY
                     SQQEGKLLIVVARKSQLQVIARAIKEVDENAFITVSQATSVYGRGFEQIKSGFKKKDN
                     ENKKQKDLIGN"
     gene            306..1262
                     /locus_tag="PLI69_04880"
     CDS             306..1262
                     /locus_tag="PLI69_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOG41857.1"
                     /translation="MKTKNKKILSEIRSYVILTIGLLFYVLAWTIFILPNNMVGGGVT
                     GISAIIQYWTGFEVGYSFFLINAVLLIVALKLLGRGFGVKTIYAIVVSSALFNIMPEL
                     IPDSFIQEIAINNGPLLCCIFGGALDGFGMALTFANGGSTGGTDIVALIINKYRNVSP
                     GRVLVVLDMIIIASSLIVPSDGTWGYKFAIVVYGYITAAVCSFTLDTFLSGTKQSVQI
                     FIFSKYYDIIADRITQEMGRGVTILNSQGWYSKEEGKVAVVVARKSESGQILRMIKEV
                     DKTAFLSVGSVMGVYGEGFEQIKSSFKKKNNESKKQKDLIGN"
     gene            1225..2127
                     /locus_tag="PLI69_04885"
     CDS             1225..2127
                     /locus_tag="PLI69_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOG41858.1"
                     /translation="MKAKSKKILSEIKSYVIITLGLLCYVLAWTIFIIPNNMVGGGVT
                     GISAIIKYWTGFEVGYSYFIINAGLLLVALKVLGRSFGVKTVFAIVVTSAFFKVVPGL
                     IPPTFIQEFAVNNGPMLCAIFGGALSGLGMALTFAEGSSTGGTDIVALMINKYRSVSP
                     GKVIVILDMIIITSSLILPSDATWGYKFAIVIYGFIMSAVLSFTLDMFLSGSKQSVQI
                     LIFSKQYDVIADRITEETGRGVTVLNSRGWYTKEEGKVAVVIVRKNESNHILRIVKEV
                     DKSAFLSVGSVMGVYGKGFEQIKK"
     gene            2264..2833
                     /locus_tag="PLI69_04890"
     CDS             2264..2833
                     /locus_tag="PLI69_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG41859.1"
                     /translation="MKLTVLILLLAYVPLFILLAYLTNYWRRNRKSEKAKEKVYPDLS
                     ERLPEKPRMLSDESPDNCEFDNLKEKLLDLFEREKIFLNSDIRINEVASRLYTNKTYL
                     SKVIRLKTNKNFCQLVHFYRVREAIRLYADNPDLTISELGKMVGFNSMTTFNTAFGRN
                     TGYTPAEWCKEYRKTLQNGEKESRKKQIA"
     gene            2796..3236
                     /locus_tag="PLI69_04895"
     CDS             2796..3236
                     /locus_tag="PLI69_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG41860.1"
                     /translation="MGRRRAEKSRLRELIKGNSHLKIMISRKRQGVSYEELFSKMEEV
                     IVKKKLYLDSGFSRSQLAREVGSNRTYVTKALKSQGVGFYDYLNRFRLERAKQLLSDG
                     ENSTMLMEEVACSSGFSSVRVMNFYMVQSEGFTASQYRKKILKQ"
     gene            complement(3226..>3634)
                     /locus_tag="PLI69_04900"
     CDS             complement(3226..>3634)
                     /locus_tag="PLI69_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG41861.1"
                     /translation="LRQRSRPYLFSNSIPPMIAAAGIRMFEMMTENNTLQDKLHANTE
                     YFVARMKAAGFDIKPTESAICAVMLYDAKLSQIMASKLLDKGIYVIGFYYPVVPKGEA
                     RIRVQVSAAHEKEHLDQCIAAFTEIGRELGVIA"
BASE COUNT         1038 a          697 c          861 g         1038 t
ORIGIN      
        1 ggattccata cttacgggca acaaacagtc tgtccaaatt atgatcttct cttccaaata
       61 tgctgaaatt gcagatatga tcaacaccaa gctcaatagg ggcgttaccg ccctaaattc
      121 catagggtgg tattctcagc aggagggtaa gctgctgatc gtggttgcgc gaaaaagcca
      181 gttgcaggtg atcgcaaggg caatcaaaga ggttgatgaa aatgcattca taacggtctc
      241 tcaggctaca agtgtttacg gcagaggttt cgagcagata aagagcggtt tcaaaaagaa
      301 ggacaatgaa aacaaaaaac aaaaagatct tatcggaaat tagatcctat gtaatactta
      361 ccataggtct tttgttctat gtgctggcct ggaccatatt catcctcccc aacaatatgg
      421 tgggaggtgg agttaccggt atttcagcca tcatacagta ttggaccggc tttgaggttg
      481 gatattcctt ttttttgatc aatgcggtgc tgcttatagt ggctctaaag ctactcggac
      541 gcggttttgg agtgaaaact atctatgcca ttgtggtgag ctctgcgctt ttcaatatta
      601 tgccggaatt gattcccgat tctttcattc aggaaattgc cataaataac ggtccgttgc
      661 tttgttgtat tttcgggggt gcgcttgatg gattcgggat ggcactgaca ttcgccaacg
      721 gaggcagtac cggaggtacc gatatagtgg ctttaataat caacaaatac agaaatgtct
      781 ctccgggaag ggtactggta gtgttggata tgataattat agcctcttcc ctgattgtgc
      841 catctgacgg cacctggggc tataaatttg ctatcgttgt atatggttac ataacagctg
      901 ccgtatgtag ctttaccttg gatacatttc tttcggggac caagcagtcg gttcagattt
      961 tcattttttc aaaatattat gatataattg ctgaccgcat cactcaggaa atggggcgtg
     1021 gagttaccat tctcaattca caaggctggt acagcaagga ggaggggaag gttgcagtag
     1081 ttgttgcgcg caagagtgaa tcgggacaga ttctccgtat gataaaggag gtggacaaaa
     1141 cggcatttct ctccgtaggt agcgtcatgg gtgtatatgg cgagggtttt gaacaaataa
     1201 agagtagttt caaaaagaaa aataatgaaa gcaaaaagca aaaagatctt atcggaaatt
     1261 aagtcctatg ttattattac attaggcctt ttatgttatg tgctggcctg gacaatcttc
     1321 attattccca acaatatggt gggtggagga gttaccggta tttcggctat cataaaatat
     1381 tggaccggct ttgaggttgg ttactcctat ttcatcatca atgcaggtct gctgttagtg
     1441 gctctcaagg tgcttggacg cagttttgga gttaaaaccg tttttgcaat agtggtgact
     1501 tcggcgtttt tcaaggttgt tccgggcctc ataccaccaa cgtttattca ggaatttgcc
     1561 gtcaataacg gtcccatgct gtgtgcaatt ttcggaggcg ctctttccgg acttggaatg
     1621 gcgctcacat ttgccgaagg gagcagcacc ggaggaaccg atatagttgc cctgatgata
     1681 aataagtata ggagcgtctc tccgggcaag gtaatcgtaa ttttggatat gattatcatt
     1741 acttcttcct tgattttacc ttctgatgca acctggggct acaaatttgc cattgtcata
     1801 tatggcttca taatgtctgc cgtattgagt ttcactttgg atatgttcct ttccggaagc
     1861 aaacagtcag tacagattct cattttttcc aaacaatatg atgtcattgc cgacagaatt
     1921 acagaggaaa caggtcgtgg tgtaaccgta ctcaattcac gtggctggta tacaaaagag
     1981 gagggcaagg tagcagtagt gattgtccgt aagaacgaat caaatcacat tctccgtata
     2041 gtaaaggagg ttgataagag tgcatttctc tctgtgggca gcgttatggg tgtctatggc
     2101 aagggttttg aacaaatcaa gaaatagtct cagttttatt aaaaacgaat tttttccgat
     2161 tttcacactt gatctaaaag tgtgatgcca ttattaggtc atatttgttg gctttatatc
     2221 tgttttgtat agtttcgttg cagaaataat aaaaacatgc aacatgaaac tgacagtatt
     2281 gatcctttta ctggcctacg tgccgctttt tatccttctg gcatacctga cgaattattg
     2341 gagaagaaat cgcaagagtg aaaaagcaaa ggagaaagtc tatccggatc tgagtgaaag
     2401 acttccggaa aagccccgca tgctgtctga tgaaagccct gacaattgtg aatttgacaa
     2461 tctcaaggag aaattgctcg atttgtttga aagggagaag atttttctca attccgatat
     2521 cagaatcaac gaagtagctt cacgccttta caccaataaa acatatctct ctaaggtaat
     2581 aagactcaaa accaacaaaa atttttgtca gttggtacac ttttatcgtg taagagaagc
     2641 cattcgtctc tatgcggaca atcctgatct gacaatctca gaattgggta aaatggtggg
     2701 ttttaattcg atgaccacat tcaacacggc atttgggagg aatacgggct atactccggc
     2761 tgaatggtgc aaggaatatc gtaaaacact acaaaatggg gagaaggaga gcagaaaaaa
     2821 gcagattgcg tgagcttata aagggcaaca gtcacctcaa gataatgata agtcgaaaac
     2881 gacaaggtgt ctcatacgaa gagctcttct ccaaaatgga agaggttata gtgaagaaaa
     2941 aactctacct cgacagcggt ttttcgagga gccaacttgc ccgtgaggta ggatccaaca
     3001 ggacctatgt caccaaagct ctaaagagtc agggagtggg attctatgac tatctaaaca
     3061 gatttcgtct ggagcgcgcc aagcagcttc taagtgatgg agagaatagt acaatgctta
     3121 tggaagaggt ggcctgcagc agcggcttct ctagcgtaag ggtaatgaac ttctatatgg
     3181 ttcaatcgga aggctttaca gcctcacagt atcgcaaaaa gatacttaag caataacccc
     3241 aagctcgcgt ccaatctcag tgaatgcggc gatacactga tcgaggtgct ctttttcgtg
     3301 agcagcagat acctgtaccc ttatgcgagc ctctcctttt ggaactaccg gataatagaa
     3361 tcctattaca tatatccctt tatcgagaag tttggaggcc atgatttgcg agagtttggc
     3421 atcgtatagc attactgcgc atatagccga ctccgtaggt ttgatatcaa atccggctgc
     3481 cttcattctt gcaacgaagt attcggtgtt agcgtgaagc ttatcctgga gggtattgtt
     3541 ctcagtcatc atctcaaaca tcctaatgcc ggcagcagct atcataggtg gaattgagtt
     3601 ggaaaagaga tagggccttg aacgctgtct gagc
//
LOCUS       DAOSIE010000141         3633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_22444_length_3633_cov_3.844885, whole genome shotgun sequence.
ACCESSION   DAOSIE010000141 DAOSIE010000000
VERSION     DAOSIE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22444_length_3633_cov_3.844885"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLI69_04905"
     CDS             complement(<1..534)
                     /locus_tag="PLI69_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41862.1"
                     /translation="MLFLFAFEAVGVRSVPFPVKVFQPDGSYLMLRIHGDESFSYKTT
                     LDGYIVAQATDGYYYYADYNNGALNISARRVDGYPTGGYAKSIPPITFHSKRHIVSES
                     FKNFLAPDVATKAVRTIRTVVIPVQFSDLKFTTQSIRSRLFNLFNQINYSEDGATGSV
                     REYFRDNLGDRYELTFEI"
     gene            656..1402
                     /locus_tag="PLI69_04910"
     CDS             656..1402
                     /locus_tag="PLI69_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG41863.1"
                     /translation="MNPTSKYQFTIIVPFFNERENIPRLEEKLSDYLKKSSCKSTCVL
                     FVNDGSTDGGDLLVQEVCSRNHAFHFISLEKNTGLSGALKAGIDHCESPYIGYIDADL
                     QTDPEDFDLLLAERENYQLVLGIRAERRDSFVKRVSSRIANSFRRMMTKDGVSDTGCP
                     LKVMQSSYARKIPFFSGMHRFLPALIQMVGGSVLEIPVRHYPRTAGKAKYNLRNRLVG
                     PFKDCFAFRWMKKRYIDYKVVAGSLNQKQS"
     gene            1402..2061
                     /locus_tag="PLI69_04915"
     CDS             1402..2061
                     /locus_tag="PLI69_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HOG41864.1"
                     /translation="MESTPFVYALGFLAQGLFSARILVQWIMSERARKVVSPTIFWVL
                     SLIASYLFFIYGWLRDDFSIMLGQVIAYYVYIWNLGAKGVWKSIHRGWRQIVITVLLL
                     TPVVGIALMAGHAQDLITRLFDNTDIPIWLILFGSLGQVIFSFRFIYQLIYSSRKGDS
                     ILPAGFWIISLTGSAIIVTYGIIRLDPVIILGQGIGFISYSRNLILWRKNNRESKSSA
                     S"
     gene            2019..3377
                     /locus_tag="PLI69_04920"
     CDS             2019..3377
                     /locus_tag="PLI69_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOG41865.1"
                     /translation="MEKKQQREQIVRFIIYFLILLPLMLQRDITPNNELKYLSIADEA
                     LRDGHFFTMYNHGETYADKPPLYIWIVMFFKWLLGSHNTFILALFSLIPSFVTLAIFN
                     RWCSGELGKKYMIAAELALMTTAYFIGSAIVLRMDMLMTMFITLAMYTFWRIYKGDDR
                     RRLRILFPVYIFLAIFSKGPVGIMIPLLCIPPFLLVEGKIRSIGRYWGWSTWLILALL
                     CGGWWLGVWFEGGHDYLNNLLFHQTVDRAVDSFHHKAPFWYYAYTIWYAMGPWSLITI
                     PVIIWALVKKMEMSSLVKFMLIVAASFLVIMSLISSKLAVYIMPIFTFLTYPAFILLR
                     NCGKPRFAEGTRKVVTYGAATLLGFFLIAGFIIPRFNSRLGYKAVAHEALEVAQANSI
                     TRFGYYDLRSAENMDVFLGRSLEELSQEEFETPRTILAPNEGIIIFHKNENGVATFKT
                     VK"
     gene            3430..>3633
                     /locus_tag="PLI69_04925"
     CDS             3430..>3633
                     /locus_tag="PLI69_04925"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOG41866.1"
                     /translation="MSANIPHAQLQLSRSGMVKNYKYFRSLLKESTKLLILVKANSYG
                     HGAVEFARAMEEAGADYFGVATPI"
BASE COUNT          998 a          799 c          812 g         1024 t
ORIGIN      
        1 aatctcaaat gtaagctcat atctatctcc gaggttgtcc ctaaaatact ccctgacact
       61 acctgtagca ccgtcttcag agtagtttat ctgattgaac aggttaaaga gtcgcgacct
      121 gatggattgt gtcgtaaatt tcagatctga aaattgtacc ggaataacta cggttcggat
      181 cgttctgacc gcttttgtag ccacatcagg agcaagaaag tttttgaaag actccgaaac
      241 tatgtgtctc ttggagtgaa atgttatggg aggtatggat tttgcgtatc cgccggtagg
      301 ataaccgtcc actcgtctgg ctgaaatatt gagcgctccg ttattatagt cggcatagta
      361 ataatagccg tcggtagcct gagccacaat ataaccgtca agggtagttt tgtaggaaaa
      421 tgattcatca ccgtgtatcc tcaacataag ataggaaccg tcaggctgaa acaccttgac
      481 gggaaatggc acacttctga ctccgactgc ctcgaaggca aataaaaaga gcattgttac
      541 tattacggcg aggtttcgca tacactaatt acgcggacga attaaaacta cataattaca
      601 aaaaataatt gaattttcaa aaaacataat cctaactttg cacaaacaat aggaaatgaa
      661 cccaacctca aaatatcaat tcacaataat cgtcccattc ttcaacgaaa gggaaaatat
      721 cccaagattg gaagaaaaac tctctgatta tctaaaaaaa tcctcatgca aatcaacctg
      781 tgtactcttt gttaatgatg gttctaccga cggaggagat cttctggttc aggaggtctg
      841 tagcagaaat catgccttcc acttcatttc gctggaaaag aacaccggcc tgagcggtgc
      901 tctcaaagcc ggtatcgacc actgtgagtc accttatatc ggctatatag acgcagatct
      961 gcagacagat ccggaggatt ttgacctatt gttggcagag cgcgagaatt atcagcttgt
     1021 acttggaatt agagcggagc gcagggattc cttcgttaag agagtctctt cccgtatagc
     1081 caacagtttc cgcaggatga tgacaaaaga tggagtctca gacacggggt gccctctcaa
     1141 agtaatgcag agtagttatg cccgcaagat ccccttcttt agcgggatgc accgctttct
     1201 gccggcattg atacagatgg ttggaggctc tgtactggag attcccgtca gacactaccc
     1261 ccgtacagcg ggaaaagcca aatacaacct tagaaaccgc ttagttggac ctttcaagga
     1321 ttgtttcgcc ttccgctgga tgaagaaaag atatatcgac tacaaggttg tcgccggtag
     1381 tcttaaccaa aagcagagct gatggagagt actccctttg tatatgcgct aggttttttg
     1441 gcccaaggac tcttttcagc aaggattctg gtgcagtgga tcatgtctga aagagccagg
     1501 aaagtggtct ctcccaccat attctgggtt ctttcgctga tagcctcata cctgtttttc
     1561 atatacggat ggcttcggga tgacttttct ataatgctcg gacaagtgat cgcgtattat
     1621 gtctatatct ggaatctagg agcaaaagga gtctggaaga gtatacacag gggatggaga
     1681 caaatagtaa taacggtact gctactaacc cctgtagtag ggattgctct gatggcaggc
     1741 catgcacagg atctgatcac gcgacttttc gacaatactg acattccaat atggctgata
     1801 ctcttcggat cattaggaca agtgatattt tccttcagat tcatctatca acttatatat
     1861 tcctcccgca agggagattc gatacttcct gcaggatttt ggatcatcag tctcacagga
     1921 tctgccatca tagtcaccta cggtataata cgtcttgacc ctgtaataat tctcggtcag
     1981 ggcatagggt tcatttcgta ttcacgcaac ctgatcttat ggagaaaaaa caacagagag
     2041 agcaaatcgt ccgcttcata atttactttc tgatacttct gccgcttatg ctccaaaggg
     2101 acattacccc taacaatgag cttaagtatc tgagcatcgc agatgaagcg ctgcgtgacg
     2161 gccatttctt tacaatgtac aatcatggag aaacctatgc tgataagcca cctctctata
     2221 tctggattgt gatgtttttc aaatggcttc tgggcagcca taacaccttt attctcgctc
     2281 ttttctccct gattccgagt tttgtcactc tggcaatatt caaccgctgg tgcagtgggg
     2341 agcttggcaa gaagtatatg attgcagcag aactggcgtt gatgacaaca gcctatttta
     2401 taggcagtgc catcgttttg aggatggata tgctgatgac tatgtttatc actcttgcca
     2461 tgtacacatt ctggaggatc tacaaagggg atgacaggag aaggctaagg attctctttc
     2521 cggtctatat ttttctggcc atcttctcta aaggacctgt tggaattatg attcccctgc
     2581 tctgcatacc gccgttttta ctggttgaag gtaagatcag aagtatcgga agatactggg
     2641 gatggagtac ctggctcatc ctggctctgc tgtgcggggg atggtggttg ggagtgtggt
     2701 ttgagggggg gcacgactat ctcaacaatc tactattcca ccagacagtt gaccgtgcgg
     2761 tagactcttt ccaccacaaa gctcccttct ggtactacgc gtataccatc tggtatgcta
     2821 tgggaccgtg gtcactaatt accattcctg ttattatttg ggccttggta aaaaaaatgg
     2881 agatgagttc gctggtgaaa ttcatgctga ttgtcgcagc ctcgtttctt gtgataatgt
     2941 ctctgataag ttctaaactt gcagtgtata taatgccgat attcacattc ctcacctatc
     3001 ctgccttcat tctgctgcgg aactgcggta aacctcgttt tgctgaaggt acaaggaagg
     3061 ttgtgaccta cggtgcagct actttgctgg gttttttttt aattgccgga ttcatcatac
     3121 cccgtttcaa cagtcgtcta ggttacaaag ctgtggcgca tgaggcactt gaagtagcac
     3181 aagcaaactc cattacccgt tttggatact acgacctgcg ctctgctgag aatatggacg
     3241 tctttcttgg cagaagtctg gaggaacttt cgcaagaaga atttgagact ccccgcacta
     3301 tcctcgctcc aaacgaagga attattattt ttcacaaaaa tgaaaacggt gttgccactt
     3361 tcaagaccgt aaagtgataa caaggagatt attttataac tttgtccgat tgaaaacaaa
     3421 ccatattaaa tgtcagcaaa tataccccac gcccaactgc agctcagtag aagcggaatg
     3481 gtaaaaaact ataaatattt ccgctcctta ctaaaagaga gtaccaagct gcttattctt
     3541 gttaaagcca actcctacgg acacggtgca gtcgaatttg cccgtgcaat ggaagaggca
     3601 ggtgcagatt attttggagt ggctactccc att
//
LOCUS       DAOSIE010000142         3604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_22651_length_3604_cov_4.961398, whole genome shotgun sequence.
ACCESSION   DAOSIE010000142 DAOSIE010000000
VERSION     DAOSIE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22651_length_3604_cov_4.961398"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1434)
                     /locus_tag="PLI69_04930"
     CDS             complement(46..1434)
                     /locus_tag="PLI69_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOG41867.1"
                     /translation="MELPFAESYKIKTVENLKRSTREERDKWIAEAKYNLFNLKSDYV
                     FIDLLTDSGTGAMSDKQWAAIMEGDESYAGARSYYALKESIEEVTGFRYFIPTHQGRA
                     AENVLFSAIIKEGDILPGNSHFDTTKGHIEFRKAFAIDCTIDEAKDTELEIPFKGNID
                     LDKLEKVLKSNPVEKIPAIVLTVTNNTAGGQPVSMANVKGVAALAKKYGVKLLIDSAR
                     FAENAYFIKTREGGYQDKEIREITREMFSYADMMTMSSKKDAIVNIGGFVAFRAEEDM
                     QKCHMYCIMNEGYLTYGGQAGRDMNALAQGLREGTEFDYLETRIRQVEYLGKKLDEYG
                     IPYQRPAGGHAIFVDAKKILTKVPKEEFIAQTLGIELYLEAGIRSVEIGALLADRDPV
                     TKENRYPALELLRLAIPRRVYTNNHMDYIAAALKNVYDRRNSITRGYVITKELPIMRH
                     FTVELAPAPRTV"
     gene            complement(1534..>3604)
                     /gene="secD"
                     /locus_tag="PLI69_04935"
     CDS             complement(1534..>3604)
                     /gene="secD"
                     /locus_tag="PLI69_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOG41868.1"
                     /translation="EKANPLFAKLQPFGGNTACIGIAHYRDTADINAWFRNSHVEFPN
                     DFVPAWGFKAFSGPGVEPDTFFELVALKDIAGKGAVLDGSVITSARVNYNQMTAAAEV
                     SMTMNSSGAARWEVITEQNIGKQIAIVMDGSVYSYPNVQNKISGGNSQITGHFTQSEA
                     DDLATVLKSGKLSTPARIVQEQVVGPSLGSASIKSGMISFAIAFLLVLIYMALFYRRA
                     GIYADVALLCNVLFLFGALVSFGAVLTLPGIAGLVLTMGMAVDANVIIYERVKEELKI
                     GKALRLAIADGYKNAYSAIIDGQLTTIITGIVLFFFGSGAVKGFAAVLIIGIITSVLT
                     SIFISRLLMEARLKRNKDISFDSPATKNFLKNTSVNFLGLRKWAYIASGVVILICFVS
                     FFTKGFTYGVDFSGGRTYVIRFDSPVTPEDVRAATMEEFGEAAEVKQFGGASQMRVTT
                     KYKVSDQSQEVDKEVEAKLYEALKDFYVTPLTLDGFTSTLENPNGIISSDRVDASVAS
                     EMQRDAIISVVLALIAIFGYIAIRFRNWTWGLGGVSSLIHNTIIVVGFFSIFTGVLPF
                     TLDVDQQFIAAVLTIIGYSINDNVVIFDRIREYRGLYPKRPLDQNVNEALNSTLSRTI
                     NTSLTTLLVLVAISIFGGESIRGFAVALAIGIIVGTYSSIFVGTPIMYDLNTRKEKRL
                     AAKTAKK"
BASE COUNT          957 a          858 c          803 g          986 t
ORIGIN      
        1 aagtttaaca aaatgtgtca acttttttca ggacgagaca gcgggttaca cggtgcgggg
       61 tgcgggggcc agctctaccg taaagtgtct cataataggc agctctttcg ttatgacata
      121 acctcgggtt atgctattcc tgcgatcata tacattttta agagcggcgg ctatgtagtc
      181 catgtggttg ttggtataga cacgacgggg aatggctaga cgtagcaatt ccagtgcagg
      241 ataacggttt tctttagtca ccggatcacg gtctgccaga agtgctccga tctccacact
      301 gcgtatacct gcctccaggt agagctcaat accgagcgtc tgagctataa actcctcttt
      361 tggcaccttt gtgagtatct ttttggcatc tacgaaaatt gcgtgtccgc ctgcgggtct
      421 ctgatatgga ataccatact catccagttt tttacccaaa tactccacct gccttattcg
      481 tgtttcgaga tagtcaaact cggtaccttc gcgcaatcct tgtgcaagcg cattcatatc
      541 cctgccggct tgtcctccgt atgtgagata accttcattc ataatgcagt acatgtggca
      601 tttctgcata tcctcttcag ccctgaaggc cacgaagcca ccgatgttta caatggcatc
      661 cttcttagat gacatcgtca tcatgtccgc ataagaaaac atttcacggg taatctccct
      721 gatttcctta tcctgataac ccccttcacg ggttttgatg aaatatgcat tctctgcaaa
      781 ccgggctgag tctataagaa gtttcacacc atattttttt gccaaagcag ctacgccttt
      841 gacatttgcc atggagacag gttggcctcc ggctgtgttg ttggtaacag taagcacaat
      901 tgcgggaatc ttctctacgg gattgctctt taagaccttc tccagtttat caagatcaat
      961 attgccctta aaggggatct caagttccgt atctttggct tcatctatag tgcaatcaat
     1021 tgcaaaggcc ttgcggaatt ctatatgacc tttagtcgta tcaaaatgag agttaccggg
     1081 aaggatgtct ccctctttta taattgccga aaaaagcacg ttttcagccg ctctgccttg
     1141 atgcgtgggt atgaaatacc gaaaccccgt cacctcttca atcgactctt ttagggcata
     1201 ataggacctt gcgccggcat aactttcatc accctccata atggccgccc actgtttgtc
     1261 actcatcgcc cctgtacccg aatctgtcag aaggtcaatg aatacatagt cgctcttgag
     1321 attaaaaagg ttatactttg cctcagcgat ccacttatcc ctctcttcgc gagtgctgcg
     1381 cttgagattt tcaactgttt ttatcttata agattctgca aaaggtaact ccatgattac
     1441 aatttttaaa gtttaacggc cacaaagtta caaaaaataa gagcaaaccg aagatttgct
     1501 cttatttgaa attaaaatta tttttccgga agattacttc ttcgcagttt ttgctgcaag
     1561 cctcttttct ttacgggtat ttagatcata catgatggga gttccaacaa atatggatga
     1621 gtaggtacct acaataatac cgatagcaag tgcaacggca aatccgcgta tgctctctcc
     1681 accgaaaatg gaaattgcta caagcacgag caaagtggtc agtgaagtgt ttatcgttct
     1741 ggaaagcgta ctgttcagag cctcgtttac attctgatcc agcgggcgct tgggatagag
     1801 tcccctgtat tcacggatac ggtcgaagat cactacgttg tcattgatgg agtaaccgat
     1861 gatggtcagt acggctgcaa taaattgctg gtcaacatcg agggtaaagg gaagtacgcc
     1921 tgtaaagatt gagaagaatc ccactactat tatggtgttg tgaataagtg aagaaacacc
     1981 gccaagaccc caggtccaat tgcggaatct gatcgctata taaccgaata tggctataag
     2041 ggccagaaca acggaaatga tggcatctct ctgcatttca cttgcaacag acgcgtccac
     2101 gcggtccgaa ctgatgatac cgttggggtt ttcaagagta gaagtgaaac catcaagggt
     2161 aagtggtgtt acatagaaat ctttgagagc ctcatagagt ttggcctcta cctccttgtc
     2221 aacctcttga gactggtcgc tcactttata tttcgtagta accctcatct ggctggcacc
     2281 tccgaattgt tttacttctg ccgcctcgcc aaattcttcc attgttgccg cacgcacatc
     2341 ttcgggagtt accggggagt caaaccttat cacataagta cgtcctccgc tgaaatccac
     2401 accgtaggta aagccttttg taaagaatga tacgaaacaa atcaaaatca caactcctga
     2461 ggcaatgtaa gcccactttc tcaaaccaag gaaattgacg ctcgtattct tgaggaagtt
     2521 ctttgtagcg ggtgaatcga atgagatatc cttattcctc ttgagtctgg cttccatcag
     2581 aagacgtgag atgaagatgg aggtcagaac tgaagttatg atacctatga tcaacactgc
     2641 tgcaaagccc ttcactgcgc ctgaaccgaa gaagaagagc acgattcccg taataatggt
     2701 ggtcaactga ccgtctatga tggccgagta ggcattcttg tatccgtcag caatagccag
     2761 gcggagtgcc ttacctattt tgagttcctc tttaacgcgc tcataaataa taacgttggc
     2821 gtctaccgcc atacccatgg tcagcacgag accggcgata cccggcaatg taagtactgc
     2881 accaaatgag acgagtgcgc cgaagaggaa gagtacgtta cagagcagtg ctacgtcagc
     2941 atagataccg gctctcctgt agaagagtgc catatagatc aatacgagca ggaatgcgat
     3001 tgcaaatgag atcatacccg atttgatgga agcactaccg agtgaaggac ccacaacctg
     3061 ctcctgaact atgcgtgcag gagttgagag cttaccggac ttgagcacgg tggcaaggtc
     3121 atcagcctca gactgggtaa agtgtcctgt aatctgagag ttacctccgg aaattttatt
     3181 ctgtacgttg ggatatgaat atacggaacc atccatgacg atggcaatct gctttcctat
     3241 attctgctcg gtaattacct cccatctggc ggcacctgag ctgttcatag tcatgctcac
     3301 ctcagcagcg gcagtcatct gattgtagtt gacccttgct gatgtgatga ccgaaccgtc
     3361 cagaactgcc cccttgccgg ctatatcctt aagagctaca agttcaaaaa aagtatcagg
     3421 ctcaacacca ggaccgctga atgccttgaa gccccacgcg ggtacaaagt cattggggaa
     3481 ctctacgtgt gaattgcgaa accaggcgtt gatatcggca gtatcacgat agtgtgcaat
     3541 accaatacaa gctgtgttac cgccgaatgg ctggagtttt gcaaagagag gattggcttt
     3601 ctcc
//
LOCUS       DAOSIE010000143         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_22748_length_3591_cov_3.813914, whole genome shotgun sequence.
ACCESSION   DAOSIE010000143 DAOSIE010000000
VERSION     DAOSIE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22748_length_3591_cov_3.813914"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLI69_04940"
     CDS             complement(<1..516)
                     /locus_tag="PLI69_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG41869.1"
                     /translation="MKQGVLVTGGTGYIGSHTVVEMIQAGYEVIIVDNLSNSEESVID
                     GIEAITGGRPDFVKADCRDIVALEELFDKGKFNSIIHFAAAKAVGESVEKPLDYYHNN
                     LLSLINILSLMRKYNVSNLVFSSSCTVYGQPDVLPATEQTPRLEAESPYGNTKRISED
                     IIRDSVKAYGTI"
     gene            622..1968
                     /locus_tag="PLI69_04945"
     CDS             622..1968
                     /locus_tag="PLI69_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41870.1"
                     /translation="MKRFTQVALVLSIIMMLASPSEVWGQNRRTSSSSSSSERKTTQS
                     SNSSNSRSSSSSSGSRSSSSQSRTISSSRSGSSQDRSTSSSSQRRTGVKASSDNTFDF
                     KVDPNYKSPSSSTQSQSTTTRRRTTTTSDRQVQDYRGTSGSNAGSRSSTVTTRRSSSS
                     SSAVTGSDNAATRRSSTSSRQAVTTDRNRSDASSGTGRSSVSTTTRRNNTSVNSTVPR
                     RSTDRVNAPATDRKPAAVQYHNSVPPPRDKNWVRPYIKKHYKPVPSYHYGHHYFGYRI
                     QYLPYRAVLRIHNHIHYYYYDGIYYRPYYGGGYVIARPPVGTYISNTMLNVALTVAVI
                     NALNDAVYRANRAVALAERYSRINTSYNIRNVDSYVTNVASQNEEYYYQDGVFYTLSD
                     GHYYVIEPPIGALITQLPEDYVEVEIGGETYYQVEDALYKVTVIDGALYFEVVLAL"
     gene            1986..2996
                     /locus_tag="PLI69_04950"
     CDS             1986..2996
                     /locus_tag="PLI69_04950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG41871.1"
                     /translation="MDCAIVILNWNGRNYLERYIPTLLNCMTGDHCFVVVADNGSEDG
                     SVEWLKNNHPSIRVIEFDRNWGFTGGYNRALAEIEADYYVLLNSDVEVTPGWADTLLS
                     FMEEHPEVGVCQPKVLSVADKDSFEYAGAAGGFLDKYGYPFCRGRILSNIEKDYGQYD
                     QPKEVFWATGACLMIRSSLYHHLGGLDELFFAHMEEIDLCWRAKLLGYEVWAIPSSKV
                     YHVGGGTLPNESPHKLYLNYRNSLLMLWKNLPEKIRRRRMLVRKCLDGVSALIYLITG
                     KMESLKAVWRAHRDYYRMKRELDPSPFSEEINNKGYYPYSIVLQFYLSRGKITFDKLK
                     NL"
     gene            complement(3006..3257)
                     /locus_tag="PLI69_04955"
     CDS             complement(3006..3257)
                     /locus_tag="PLI69_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG41872.1"
                     /translation="MIDKRLQVFAVAARQLSFVKTAKILGISQPAVSKHIALLEKELG
                     GALFLRISQTLVLTKKGEELLEIADKILALYQSIDTLKD"
     gene            3340..>3591
                     /locus_tag="PLI69_04960"
     CDS             3340..>3591
                     /locus_tag="PLI69_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG41873.1"
                     /translation="MRNLISTILSLFVTFISFAQVNPQGLLERDPSVRYGKLDNGLTY
                     YIKHNNKPAQRADYYLFTNVGAIQETPAQNGLAHFLEHMA"
BASE COUNT         1022 a          828 c          779 g          962 t
ORIGIN      
        1 gatcgttccg taggctttga cgctatcacg gattatatcc tcggaaatgc gttttgtgtt
       61 gccataggga gactctgcct caagtcgcgg agtctgctcg gtagcaggta gtacatcagg
      121 ctgtccatag acggtacacg aggaggagaa aaccagattt gatacattgt atttccgcat
      181 cagcgaaaga atattgataa gggagagaag attgttatgg taataatcta gaggcttttc
      241 gactgactcc cctactgcct ttgccgctgc aaagtgtata attgagttaa atttcccttt
      301 atcaaaaagc tcttcaagcg ctacaatatc acgacagtca gctttgacaa aatccggcct
      361 gccgccggta atggcctcta tgccatcaat taccgattct tcggaatttg acagattgtc
      421 gacaataatc acctcataac ccgcttgaat catctctaca acagtgtgtg agcctatata
      481 acccgttcct ccggtaacca atactccttg cttcatatta ccataatttt aagctgatgc
      541 aaaaataaga aaaaagagtt atatttggtc cgttatttga taatcattaa ggtgaatact
      601 caccatttaa atttttaaaa aatgaagagg tttacacaag tagctttagt ccttagcatc
      661 ataatgatgt tggctagccc tagtgaggta tggggacaaa ataggagaac gagcagttcg
      721 agcagttcga gcgaacgtaa aaccactcag agcagcaact cttccaatag tcgctcaagt
      781 agctcttcat ccggcagccg ttcgagtagt tcgcagagtc gaactatttc cagcagccgt
      841 tcaggcagct cacaggaccg tagtacttca agttcttcac agagaaggac aggagtgaag
      901 gcttcatcgg acaatacatt cgacttcaaa gtcgatccca actacaagtc accctcctca
      961 agtactcagt cacagagtac gacaacccgc aggaggacta caactacttc tgaccgtcag
     1021 gtgcaggatt acagaggtac ctccggttcc aatgcaggct cccgcagctc cacagtcacc
     1081 acaagacgta gctcaagcag ctcttctgca gtgacaggtt cggacaatgc ggctaccaga
     1141 aggagttcta cctcaagcag acaggctgta actacggacc gcaacagatc tgatgcaagc
     1201 tccggtaccg gcagatcatc tgtttcaacc accacccgca ggaataacac cagtgtaaac
     1261 agcacagttc caaggaggag cacagatagg gttaatgcac ctgcgactga ccgtaagccc
     1321 gcagccgtgc aatatcataa cagcgtacct ccgccaaggg ataaaaattg ggttagaccc
     1381 tatatcaaga agcactacaa gccggttcct tcttatcact acggtcatca ctacttcggt
     1441 tacagaattc aataccttcc ttatagagcg gtactccgga ttcataacca catccactac
     1501 tactattatg atggcatcta ttaccgcccc tactatggag gaggatatgt gattgcccgt
     1561 ccaccggtag gtacatacat tagcaacacc atgctcaatg tggctcttac agtagcggtt
     1621 ataaatgccc taaacgatgc cgtatatcgc gcaaatcgtg cagttgcact agctgagaga
     1681 tactcaagga tcaatacctc ctacaatata aggaatgttg actcttatgt gacaaatgta
     1741 gcgagccaaa atgaagaata ttactatcag gatggagttt tctacaccct ttccgacggc
     1801 cattactatg tgatagaacc tccaatcgga gctctcatta ctcagttgcc ggaagattac
     1861 gttgaggtgg agataggtgg cgagacttac taccaggtcg aagacgcact ctacaaagtg
     1921 acagttatcg acggagccct ctacttcgag gtggttctag ccctataact ccggaatcct
     1981 cctgtatgga ttgtgctata gtcatactca attggaatgg tcggaattat cttgaaaggt
     2041 atattccgac ccttcttaat tgtatgacag gtgatcattg ttttgtggtt gtggcagaca
     2101 acggatcaga agatggatcc gttgagtggc taaaaaataa tcatcctagt atacgagtca
     2161 tagagtttga tcgcaattgg ggctttacgg gtggctacaa tcgtgccctg gccgaaattg
     2221 aggccgacta ttatgtgttg ctcaactcag atgtcgaggt aactcccggc tgggcagata
     2281 cccttctttc cttcatggag gagcaccccg aggtaggtgt gtgtcaaccg aaggtacttt
     2341 cagtggcaga caaagactcc ttcgaatatg ccggtgcagc agggggcttt ttagataaat
     2401 acggatatcc attttgcagg ggacgcatcc tttccaatat agagaaggat tacggacaat
     2461 atgaccagcc gaaagaggta ttctgggcta ccggtgcctg tctgatgatc agatcttctc
     2521 tttatcacca tctaggtggt ctggatgaac tgttttttgc ccacatggag gagatagacc
     2581 tctgttggcg agcaaaactg ttagggtatg aggtgtgggc tattccttcc tcaaaagttt
     2641 accatgtcgg cggcggtact cttcccaatg aatctcccca taagctctat cttaattacc
     2701 gcaacagtct cctgatgcta tggaaaaacc ttcccgaaaa gattcgtcgg aggagaatgt
     2761 tagttcgtaa atgtcttgac ggagtttctg ccttgatata tctcattacc ggcaaaatgg
     2821 aaagtctaaa ggccgtatgg cgagcgcatc gtgattatta ccgaatgaaa cgcgagcttg
     2881 acccttctcc attctctgaa gagattaaca ataagggcta ttatccttat agcatagtgc
     2941 tgcaattcta cctctcgcgt ggaaaaatca cttttgacaa gctcaaaaat ctttaaagta
     3001 ctcaatcaat ctttgagtgt atcgatagac tggtatagcg caagtatttt atcagctatt
     3061 tccagtagtt cttcaccctt ttttgtcaga acgagggtct ggcttattct tagaaacaat
     3121 gctccgccaa gctctttttc gagtaaagca atatgcttgg aaacagccgg ttgggatatg
     3181 ccgagaatct ttgccgtctt tacaaaactc aactgacggg cagctacggc aaacacttgc
     3241 aatcttttgt ctatcataat ggcacaaatt tagcaaaaat gaataaattt gtaagattta
     3301 gttcaatttt aaaaaacaca attataatat ttacctaaaa tgagaaattt aatttcaacg
     3361 attctgtcac tctttgtgac atttatttct tttgcacagg tcaatccgca gggcctcctt
     3421 gagagagacc catccgtgag atacggcaaa ttggataatg gattgaccta ttatataaaa
     3481 cacaataata agcctgcgca acgcgccgac tactatcttt tcaccaatgt gggtgcaatc
     3541 caggagactc ctgcccagaa tggccttgct cacttccttg agcatatggc g
//
LOCUS       DAOSIE010000144         3576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_22876_length_3576_cov_4.016473, whole genome shotgun sequence.
ACCESSION   DAOSIE010000144 DAOSIE010000000
VERSION     DAOSIE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22876_length_3576_cov_4.016473"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLI69_04965"
     CDS             <1..814
                     /locus_tag="PLI69_04965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG41874.1"
                     /translation="STDREKQLIAEGDYMEREYDRMKGQMDIVESVVEGLEARDRQIY
                     NDIFQSAPPTYMIGRDTARLDLGTIYEEDENDLIWNVNIRASRLESISGKINNSIENI
                     NHRLSAGEMDWRSIPSIIPVKDFSIARTGASVGNKFSPFYKTLRDHTGFDILSPVGTE
                     VLAAASGTVTAVTRGATAWGRRIEITHSGGIVTTYSHLQEIMVKKGQQVQQSDIIGRV
                     GNSGTSFAPHLHYEVIVNGNYVDPINYFFADITPLAYQEMMIIALSTGQSMD"
     gene            821..1141
                     /locus_tag="PLI69_04970"
     CDS             821..1141
                     /locus_tag="PLI69_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOG41875.1"
                     /translation="MTGQHYHTIGQVAKMFDVNVSLIRFWSDTFPEYVRPARNNKGNR
                     LYREADIDALRNIARLVRDQGMTLEGARNRLKFGLDEVDRRSEAIEILRNIRASLTEV
                     RDAL"
     gene            1157..1921
                     /locus_tag="PLI69_04975"
     CDS             1157..1921
                     /locus_tag="PLI69_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOG41876.1"
                     /translation="MKAKDIALIIEEFAPLGTQEPWDNSGFCIGSPEQEIKGVMIGFD
                     PTPELIEAAVAKGADMVITHHPLIFSGIKKINPDTFIGKAITLAIKHGMVIYSAHTNA
                     DKAEGGVTALMAKRLGLQDITPLDETLLALVGTLPSPVEPQALSAFVKKAFGIERLRS
                     SRLIGEPVSRVSVCAGSGSSFIPQAIEADAQLFITGDVSYHHFFCPEGFMVMDIGHYE
                     SERDIVDKLYEILSKKIPTFAIHTFLNNNNNPIFYF"
     gene            2025..2747
                     /locus_tag="PLI69_04980"
     CDS             2025..2747
                     /locus_tag="PLI69_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOG41877.1"
                     /translation="MEQKLQILYQIQNIDTKIDKIYLLRGELPLEVEDLEDEIEGLKT
                     RIANIMAEIKEIEKYNTEYKHQIEESKKAISKYEEQRNDVKNNREFESLSKEIEFQEL
                     EISVAEKNIKEGNATIADKKLLLDDSKAALAGRQVDLADKEEELKSITEETAKEEEEL
                     LDKRDSLVATLDERTINAYERVRKGAKNRLAVVTVERGACGGCFNNIPPQRELDVIES
                     KKMIICEHCGRILVKSPDKQEE"
     gene            2755..>3576
                     /locus_tag="PLI69_04985"
     CDS             2755..>3576
                     /locus_tag="PLI69_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HOG41878.1"
                     /translation="MAKKDSGKKDRKVDLDILGEELGGRVPPQAIDIEEAVLGALLLE
                     PEVVVDVLDQLSAECFYKDAHRKIYDAIATLSKRNDPVDIFTVSDELRKSGDLEEIGG
                     TAYLSQLTLKIGAAAHIDYHTKVLLQKFIQRELISISYKIQKSAFDDSMTVDDLVDTA
                     EKEVFELAERNMRRETSPIKDIVGQAIREIEINQQREDGLSGIPSGFTGIDKITFGWQ
                     KSDLIIIAARPSVGKTAFVLTMARNMAIDHNIPVAFFSLEMPATQLVKRVMIGETG"
BASE COUNT         1027 a          798 c          900 g          851 t
ORIGIN      
        1 cagcacggac cgcgaaaagc agctcattgc cgaaggggat tatatggagc gggaatatga
       61 taggatgaag ggacagatgg atattgtcga gagcgtagtc gaggggcttg aagcgaggga
      121 tcgccagatt tataatgata tcttccaatc ggctcctccg acttatatga ttggtcgtga
      181 tactgcccgc ctcgatcttg gtaccattta cgaagaggat gagaatgatc tgatctggaa
      241 tgtcaatatc cgggcatccc gcctcgaatc gatctcaggc aaaatcaata attccattga
      301 aaacatcaat caccgattat ctgccggtga aatggattgg aggtccattc cttccattat
      361 tcccgttaag gacttttcca tcgccagaac cggtgcatcg gtaggcaata agttcagccc
      421 cttttacaag acgctccggg accacacagg tttcgacata ctctctcctg ttggcaccga
      481 agtgcttgcg gcagcctccg gcaccgtaac cgcggtcacc agaggtgcaa ctgcctgggg
      541 caggcgtatt gagataaccc actccggagg tatagttacc acctattcgc accttcaaga
      601 aatcatggtt aaaaagggac aacaagtgca gcagtccgat attatcggta gagtaggcaa
      661 tagcggcacg tcatttgctc cgcacctcca ttatgaagtt atagtcaacg gcaattatgt
      721 cgatccgatc aattattttt ttgccgacat tactccattg gcatatcagg aaatgatgat
      781 aatagcgctt agtaccggac aatccatgga ttgataagat atgacaggac aacattacca
      841 tactataggc caggtggcaa agatgtttga tgtaaacgta tcacttattc gtttctggtc
      901 ggacaccttc ccggaatatg tgcgtccggc tcgcaacaat aagggcaacc gactctatcg
      961 cgaagccgat atagatgcgc taagaaatat cgcccggctg gtcagagatc agggtatgac
     1021 ccttgagggt gcccgcaatc ggttgaaatt cggattggat gaggtagaca gaaggagtga
     1081 agccatagaa atactcagga atatacgcgc ctcactgact gaggtgcggg atgcccttta
     1141 gtccccgaaa ttggatatga aagcaaaaga tatagcacta attatcgagg aattcgcccc
     1201 cctcggtacg caggagcctt gggacaactc cggtttctgc ataggctctc ccgagcagga
     1261 gataaagggt gtgatgattg gatttgaccc cactccggag ctgatagagg ctgcggtggc
     1321 caagggagcg gatatggtta taacccatca cccgttgatt ttttccggca tcaaaaagat
     1381 taatccggat acattcatcg gtaaggccat cactcttgca attaagcatg gaatggtcat
     1441 ctactcggca catacaaacg ccgataaggc tgaaggtggg gtaaccgccc tgatggctaa
     1501 acgtttggga ttgcaggata tcacgcctct cgatgagacc ttattggctc ttgtcggcac
     1561 tctaccctct ccggtggagc ctcaggcact ctccgcattt gtaaaaaagg ctttcggaat
     1621 agaacgtctg cgcagttcaa ggctcattgg ggagcctgtt tcgagggtgt ccgtctgtgc
     1681 ggggagcggt tcttcattca tccctcaggc gattgaagca gatgcccagc tttttatcac
     1741 aggagatgtc tcgtaccacc attttttctg cccggagggc tttatggtga tggatatcgg
     1801 acattatgag agcgagaggg atattgtgga taagttgtat gaaattctta gcaaaaaaat
     1861 tcctaccttt gcaatccaca ctttcttgaa taataataac aatccaatat tttacttcta
     1921 agtatatggc tacaaaaaag gcaactaaag tggttactcc tatagaccag agggaaacct
     1981 ttgtttcgct ttcgacacat tccggcgaaa ccgatgcgaa ggagatggag cagaagcttc
     2041 aaattttgta tcaaatccag aatattgaca ctaaaattga caagatatat ttgctcagag
     2101 gtgagcttcc gctcgaagtg gaggatcttg aagatgaaat cgagggcctt aagacccgca
     2161 ttgcaaatat aatggccgaa attaaggaga tcgaaaaata caacactgag tacaagcatc
     2221 agatagagga gtcaaaaaag gcgatatcca aatatgaaga gcagcgcaat gatgtcaaaa
     2281 acaatcgcga gtttgaatct ctttcaaagg aaatcgagtt tcaggaactc gagataagtg
     2341 ttgccgagaa aaacatcaaa gagggcaatg ccaccattgc tgataaaaaa ctcttactgg
     2401 atgactccaa agctgctctt gcaggcagac aagtggacct tgccgataag gaagaggagc
     2461 tcaaatccat cactgaggag acggccaaag aggaggagga gctgctagac aagcgcgatt
     2521 ctctggttgc cactctcgat gagaggacca tcaacgctta tgagcgtgtg cgtaaaggtg
     2581 caaaaaacag gcttgccgtt gtgacggtcg aaagaggtgc ctgcggaggc tgcttcaata
     2641 atattcctcc ccagagggaa ctcgatgtca ttgaaagcaa gaaaatgata atctgcgaac
     2701 attgcggtcg catccttgta aaatctcctg acaaacagga agaataagtg agatatggcc
     2761 aagaaagatt ccggtaaaaa ggataggaag gtggatttgg atatcctcgg cgaagagctg
     2821 ggagggcggg tgcctccaca ggctatcgac attgaggagg ctgtcctcgg tgcgctcctg
     2881 ctggaacccg aagtagtggt agatgttctt gatcagcttt cggcagaatg tttctataaa
     2941 gatgcacacc gcaaaatata tgatgcaata gccaccctct ccaagcgcaa tgaccccgta
     3001 gatatcttca cagtttctga cgaactgcgc aaaagcgggg accttgagga gatcggagga
     3061 acggcatacc ttagccaact taccctcaag atcggagctg cagcacatat tgattatcac
     3121 accaaagtcc ttctccagaa atttatccag agggaactca tctctatctc atacaaaata
     3181 cagaaaagcg cctttgacga ttccatgaca gtcgatgatc tggtggatac tgcggaaaaa
     3241 gaggtgtttg aacttgctga gcgtaatatg cgccgtgaga cctctcccat aaaagatatc
     3301 gtaggtcagg ctatcagaga gattgagatc aaccagcaaa gagaggatgg actcagcggt
     3361 attccttcgg gttttaccgg catagacaag atcactttcg gatggcaaaa gtccgacctg
     3421 ataatcatag cggcgcgtcc ttccgtgggt aagaccgcat ttgtgctgac tatggctcgt
     3481 aatatggcta tcgaccacaa tatccccgtg gcatttttct cacttgagat gccggcgaca
     3541 cagcttgtaa agcgggtaat gattggggag accggc
//
LOCUS       DAOSIE010000145         3575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_22886_length_3575_cov_3.090625, whole genome shotgun sequence.
ACCESSION   DAOSIE010000145 DAOSIE010000000
VERSION     DAOSIE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22886_length_3575_cov_3.090625"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PLI69_04990"
     CDS             complement(<1..1038)
                     /locus_tag="PLI69_04990"
                     /inference="COORDINATES: protein motif:HMM:NF019138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOG41879.1"
                     /translation="MGDKSLRSSRGRILMVSPSKTIIMYDILQLQSKSLQELIDIAGK
                     FNIKKPESKHEDLIYQILDEQAIQGTGRPGPKPVKKKVVKEIKQVTGGTAKTAETQSA
                     NQAIKETTKESDTKAKSNSKAKVASSVVTAKSSETVAEEKKVEKTPAESKKTGRKTKK
                     QSEETPTAAQYASEEETDGKSEKKTATGKKQTEVKSESEEKPKRGTRPRQVVKKEPVR
                     IDIENVVSQEQSEKEETTVAVTKEEPAKEKTQKQESNEREQRSKAQKGEHPHQHPEQH
                     NAKPKIEFEGIVEATGVLEIMPDGYGFLRSSDYNYLNSPDDIYVSQAQIKMFALKTGD
                     AILGEIKPPREG"
     gene            complement(1095..1472)
                     /locus_tag="PLI69_04995"
     CDS             complement(1095..1472)
                     /locus_tag="PLI69_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOG41880.1"
                     /translation="MKKVVSSPLAPAAVGPYSQAIVAGNTIYVSGQLPIDAATGEFAP
                     GGVREQVHQIFKNIGYILKEAGYDFSDVVKTTVYLTDMKDFAVMNEVYAEYYSKPYPA
                     RVAYAVAGLPKAALVEVDVIAVK"
     gene            complement(1508..>3575)
                     /locus_tag="PLI69_05000"
     CDS             complement(1508..>3575)
                     /locus_tag="PLI69_05000"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOG41881.1"
                     /translation="KLTAAKMITQPSQKTVFGPAYVVVADVPLPIVLPFGFVPEMPDR
                     ASGILVPTYGEENARGLYLKDFGYSFVIGDYFDLALTGDIYSYGSWAVRATSRYKKRY
                     SFDGSLSLTYSNDVTGERGESNFFQSKNFGVMWSHSQDAKARPGTSFRASVNFSSPSN
                     SKYNSRDISQSLQNQVSSSISYSKQWSGLSLSINALHSQNSRDSSYSITLPNLTFSVN
                     RFFPFKRKNRVGKERFYEQISFSYNTTLQNKVNFKAREVTEPGFINKMQNGMAHNFQI
                     GLPSFTLLKYLQFSPSVSYGMNWVFRENQVEYDPEKNQVVTTVSDQFSTLGVMQNFSA
                     GLSMSTRLYGMFNFGKRSKIEAIRHMITPSFGFSYKPELGTPMNGYMTYEYTDINGVE
                     RTYEYNKYAGMMYSPPAKGKTAAINFSFANNLEAKIKDAADTTGRGTRKIKLIDNLNL
                     TGSYNFLADSKRMSNINISAGTTIFQKLGINGNLSLDPYAIDHTGKRINKFQVIQNGQ
                     LARLVNASASLSFSLNGEGQGKGNDGIKDTGSKKKEANAYGYVYYHPITGEYIPGGWL
                     YYMNPNSPWSVSFNYTYSYGKSYSYANEQLQTKHNHTQTLGINGQIRITQALNVSLNT
                     GFDLTKMKITTTQLSATYDLHCFAISVSWVPTGQWQSWSFRIAAKASALADLLQFKKA
                     SSFWDN"
BASE COUNT          821 a          768 c          825 g         1161 t
ORIGIN      
        1 gccctcccgg ggaggtttta tctcccccag tatagcgtct ccggttttta gcgcaaacat
       61 cttaatctgc gcctgagaaa catagatatc atcaggtgag ttgaggtagt tgtagtctga
      121 ggaacgcagg aaaccatacc catccggcat aatttccagc actccggtag cctctacaat
      181 accttcaaac tcaatttttg gttttgcatt atgctgttcc gggtgttgat gagggtgttc
      241 ccctttctga gctttgcttc gttgttcgcg ttcgttagac tcttgttttt gagtcttttc
      301 ctttgcgggt tcttctttag ttacagcaac tgtcgtctcc tctttttcac tctgctcctg
      361 gcttactaca ttttcgatat caatgcgtac gggttctttt tttacaacct gtctgggccg
      421 tgtgcctctt ttgggcttct cttcgctctc gcttttaact tcagtttgct tcttacctgt
      481 tgcagttttt ttctctgatt tgccatcagt ctcttcttcg cttgcatatt gagcagcagt
      541 cggagtctcc tcgctttgct ttttagtctt tcttccggtt ttcttgcttt cagcaggtgt
      601 tttttcgact ttcttctctt cagctacagt ttcactgctc ttggcagtaa ccacagaaga
      661 tgcaaccttg gccttagaat tactcttggc tttggtgtcg ctttccttag tggtttcttt
      721 aatagcctga tttgcagact gtgtttcagc tgtttttgca gtaccgccgg taacttgttt
      781 tatctctttc acaacttttt tctttaccgg tttgggcccg ggtctacccg taccttgaat
      841 ggcttgctca tcaagtatct gatagatgag gtcctcatgc ttagattcag gctttttaat
      901 gttgaacttc cctgcaatgt caatcaattc ttgtagactt ttgctctgta attgtagtat
      961 gtcgtacatt ataatggttt tggaagggga gaccataagt atccggccac gtgaagaccg
     1021 taaagatttg tctcccaaaa tgaaatcgcc acaaagttag cggtttttat cgactttgca
     1081 aaaaatgtgt acaattattt tactgcaatt acatcaactt ctacaagtgc ggctttagga
     1141 agtccggcaa cagcatacgc tacacgtgca ggataaggct tgctgtaata ctctgcatag
     1201 acctcgttca taacagcgaa atctttcata tcggtaagat agaccgtcgt cttgactaca
     1261 tcgctgaagt catagccggc ttctttaagg atgtatccta tatttttaaa aatttggtgg
     1321 acttgttctc taaccccgcc aggagcaaat tcgcccgtgg cggcatcgat tggaagctgt
     1381 ccggatacat agatggtatt gccggcaacg atagcctggc tgtaaggtcc cacggctgcg
     1441 ggagcaaggg gagaggatac tacttttttc ataatttctt cgtattaatg ttgttatcta
     1501 tagtatatta attgtcccaa aaacttgagg ctttcttaaa ctggaggagg tcggctagtg
     1561 cagaggcctt tgcagcaatt ctgaaactcc agctttgcca ttggccggta ggcacccagg
     1621 atacggaaat tgcaaagcag tgcaggtcat aggttgcact caattgggtg gtcgtaatct
     1681 tcatctttgt taaatcaaaa ccggtattaa ggctaacatt gagagcctgt gtaatgcgta
     1741 tctgtccatt tatacctaat gtctgggtat gattgtgttt ggtctgaagc tgttcgttgg
     1801 catagctata actcttgccg taagagtagg tataattgaa acttaccgac cagggagagt
     1861 tggggttcat ataatatagc caccctcccg gaatatattc tccggttata gggtggtaat
     1921 agacataacc ataggcgttg gcctccttct tcttagaacc ggtatccttg atgccgtcat
     1981 tgcctttgcc ctgtccctct ccattaagtg agaaactcaa tgaggcactt gcgttgacaa
     2041 ggcgtgcaag ttgtccgttt tgaatgacct ggaatttgtt tatgcgcttt cccgtgtggt
     2101 caattgcata ggggtccaga gataaattac cattaattcc gagtttctgg aatatggtag
     2161 tgccggcact gatattgata ttggacattc tcttggagtc cgcaagaaag ttgtaggatc
     2221 cggtgagatt gagattgtct atcaacttga tttttctggt acctctgcct gtagtgtcgg
     2281 cggcatcctt gattttcgct tccaggttgt ttgcaaaact aaaattaatg gcagcggttt
     2341 tcccctttgc ggggggagaa tacatcatgc ctgcgtattt gttgtattca tacgttctct
     2401 caactccgtt aatgtcggtg tactcgtatg tcatatagcc gttcataggc gtaccaagtt
     2461 cgggtttgta actaaacccg aatgagggcg ttatcatgtg tctgattgct tctatcttgc
     2521 tcctcttgcc gaaattaaac ataccgtaga gtcgggtgct catggagagt cctgcagaaa
     2581 agttctgcat tacgcctaag gtgctgaatt gatcggagac tgtagtgacc acctgatttt
     2641 tttcaggatc atattcaacc tggttttccc ggaagaccca gttcataccg taggagacgc
     2701 tgggagaaaa ctgtaaatat ttgagcagtg taaaagaggg aaggcctatc tggaagttat
     2761 gagccatacc attttgcatc ttgttaatga atccgggttc agtgacttcc cttgccttga
     2821 agttgacttt gttctgtaat gtggtattgt aactgaatga aatctgttca tagaaccgtt
     2881 ctttgcctac tctgtttttt ctcttgaagg ggaaaaacct gttgacggag aaagttaggt
     2941 ttggtagagt aatggaatat gagctgtctc gggaattctg gctatgtagc gcattgattg
     3001 aaaggcttaa gccgctccac tgtttggaat aggatattga ggaggagact tggttttgca
     3061 gactctgtga tatatctctg gagttgtatt tactgttgga ggggctggaa aagttaactg
     3121 aagctctgaa ggaggttccc ggtctcgcct tggcatcctg ggaatgactc cacatgactc
     3181 caaagttctt ggattgaaag aaattgctct ctccgcgctc ccctgtaacg tcatttgagt
     3241 aggttagaga gagtgaacca tcgaaagaat acctcttctt gtatctggaa gttgccctta
     3301 ctgcccatga gccatacgaa tagatatctc cggtcaacgc aaggtcgaaa tagtctccta
     3361 ttacaaacga atagccgaaa tctttaaggt agagtccgcg ggcattttcc tctccgtatg
     3421 taggaacaag gattccgctg gccctatccg gcatctcagg tacaaatccg aacggaagta
     3481 caataggtag cggcacatcg gctacaacta catatgccgg cccgaaaaca gtcttctgac
     3541 tcggctgggt aatcatcttg gcagctgtga gtttc
//
LOCUS       DAOSIE010000146         3543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_23135_length_3543_cov_3.078842, whole genome shotgun sequence.
ACCESSION   DAOSIE010000146 DAOSIE010000000
VERSION     DAOSIE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23135_length_3543_cov_3.078842"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLI69_05005"
     CDS             complement(<1..228)
                     /locus_tag="PLI69_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG41882.1"
                     /translation="MRDLENKVVIVTGGKGLIGREIVKQFKERDSIVIDADISNETSS
                     DDNTFKLDLAENSSIDALINFVIGKYGRIDGL"
     gene            complement(215..1147)
                     /locus_tag="PLI69_05010"
     CDS             complement(215..1147)
                     /locus_tag="PLI69_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG41883.1"
                     /translation="MKILIIGLGSIAKKHIAAIRSIDSNASIYALRSQHNAQAYDEIT
                     NLYSFSEASSIGFDFAIISNPTSEHKHTIQKLLEFRIPLFIEKPVSSSPIDDLINSIK
                     ASKILSYVACNLRFLEVIEFLKERIESGDTPGRINEVNVYCGSYLPEWHPGTDFRECY
                     SARPEMGGGVHLDLIHELDYICWIFGMPDSYKAFCRNKSSLNIDAIDYAKYLLFYKDF
                     ACDLTLNYFRRDYKREIEILFDSVTWKVDLPKNTIVDSNGVIIFESEQSGIDTYTDQM
                     QYFINLVSNNEKDSFNDIIFANEILKLCLNYERP"
     gene            complement(1147..1875)
                     /locus_tag="PLI69_05015"
     CDS             complement(1147..1875)
                     /locus_tag="PLI69_05015"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HOG41884.1"
                     /translation="MNNNSNILITICARGGSKGIPGKNIKEVDGKPLIAYTIEAAEKV
                     KDKLSGCRVDIELSTDSEEIRKCAAEYGLKSDYQRPAYLANDTSGKVDAIRDLVFHSE
                     KKNDCKYDFIVDLDVTSPIRTTDDIVDCLNMFNEDSDLKTVFSVNPCARNPYFNQVED
                     KGDGYCKIVLGGKYLSRQAAPEVYDMNASIYVYKRPFFDTDSSSPITDKSKVYVMDHI
                     CFDLDEPHDFDYLSFLLSSKLIEL"
     gene            complement(1875..3035)
                     /gene="neuC"
                     /locus_tag="PLI69_05020"
     CDS             complement(1875..3035)
                     /gene="neuC"
                     /locus_tag="PLI69_05020"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOG41885.1"
                     /translation="MRKICFVTGTRAEYGLLSRLMRLVQGDSTLQLQIIATNMHLSPK
                     FGETYREIESDGFKIDKRVPIIDEQAEDSPVSTLMAMSRGLEGFGIAYSELKPDIIII
                     LGDRHEMLAAATAALISRIPIAHLHGGEVTEGAYDDAIRHSITKMSSLHFTSCEEYRK
                     RVIQLGENPERVFNVGSIGVENIKQIPLMGKEELESSLNFKFNDRTILVTYHPETLEG
                     DSKTGIAELLAAVSQYPELRVIFTMPNSDTGHQPIVQAIEEYVKQHPYSSKAFTSLGV
                     KRYLSALQYVKAVVGNSSSGIIEVPSFGIPTLNIGDRQKGRIASQSVVHCANDRESIS
                     KGIEHVLSPGHQTISANSINPYEKERCAETIYEIISSYDLQNCINKSFYDLK"
     gene            complement(3032..>3543)
                     /locus_tag="PLI69_05025"
     CDS             complement(3032..>3543)
                     /locus_tag="PLI69_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOG41886.1"
                     /translation="DQITVLHCNTEYPTPFKDVNLKAMLTIGDKFGVKIGYSDHTMGI
                     EVPIAAVALGAMVIEKHFTLDKKMEGPDHKASLEPDELKAMVTAIRNIELAFGTGHKS
                     ITESERKNLEAARKSIVASRQIKKGELFTEENLTVKRPGNGISPMRWDEVIGQIAKRD
                     FVEDQLIDI"
BASE COUNT          983 a          732 c          670 g         1158 t
ORIGIN      
        1 cagtccatca attcgaccat atttgccaat tacaaaattg attaaagcat ctatggatga
       61 attttcagca agatcaagtt tgaatgtgtt atcatctgag gaggtttcat tggaaatatc
      121 cgcatcaatt acaattgagt ctctctcttt gaactgtttg acgatttctc ttccaatcaa
      181 cccttttcca cctgtaacaa ttaccacttt attctcaagg tctctcataa ttcaaacata
      241 attttaaaat ctcatttgca aatataatat cattgaaaga atctttctca ttatttgaga
      301 caaggttgat gaagtattgc atctgatctg tgtacgtgtc aataccgctt tgttctgact
      361 caaagatgat aactccattt gaatcaacaa tagtgttttt aggcaggtcc accttccagg
      421 ttacggaatc aaataaaatc tctatttccc ttttatagtc acgcctgaaa taattgagtg
      481 ttaagtcgca agcaaaatct ttataaaaaa ggagatattt tgcataatct attgcgtcaa
      541 tattaagaga tgatttgttg cgacaaaatg ctttgtagct atccggcatc ccgaaaatcc
      601 agcaaatata atccagttcg tgaatcagat cgagatgaac acctcctccc atttccggtc
      661 gagcactata gcactcccta aaatcggttc cgggatgcca ctcaggcaga tatgaaccgc
      721 aatagacatt tacctcattg attcttccgg gtgtatctcc cgattcgatg cgttccttta
      781 aaaattcaat aacttcaagg aatcgcaggt tacaggccac gtatgacaat attttgctcg
      841 ctttaatgct atttatgaga tcgtcgatgg gcgaggagga taccggtttt tctataaaga
      901 gtggaattct gaattccaaa agtttttgta ttgtatgttt gtgctctgat gtcggattgg
      961 atattatggc aaaatcaaat ccaatacttg atgcctcact aaatgagtaa agatttgtga
     1021 tttcgtcata ggcctgcgca ttgtgttgtg acctcaatgc gtagatggaa gcattgctgt
     1081 caatagagcg tattgctgcg atgtgttttt ttgctataga gccaagtccg ataatcagaa
     1141 ttttcattat aattcaatta gttttgaaga gagcagaaat gagagatagt caaagtcgtg
     1201 aggttcgtcc agatcaaagc aaatgtggtc cattacatag acttttgatt tatctgtgat
     1261 aggcgaagat gagtctgtgt cgaaaaaagg acgcttataa acataaatag aggcattcat
     1321 atcgtaaact tcaggtgcag cttgccttga aaggtatttt cctccaagta cgattttaca
     1381 ataaccatcg cctttatcct ctacttgatt gaaatatggg tttctggcac aaggattcac
     1441 tgagaagact gtcttaaggt cactatcttc attaaacata ttgagacaat ccactatgtc
     1501 atctgtcgtt cttatcggtg aagtgacatc caaatccact ataaaatcat atttgcaatc
     1561 attcttcttt tccgagtgaa acacaagatc cctgatggcg tccactttgc cgcttgtatc
     1621 gttagccaga tatgccggac gttgatagtc ggatttcaga ccatactctg ctgcacattt
     1681 tcttatttct tcagaatcgg tcgagagctc tatgtcaact ctgcacccac ttaatttatc
     1741 ttttactttc tctgctgcct caatagtata agctatgagt ggttttccgt ccacttcctt
     1801 gatattctta cccgggatgc cttttgaacc tcctcttgca cagattgtaa tcagtatatt
     1861 gctgttgtta ttcattattt caaatcataa aaactcttgt taatgcagtt ttgaagatca
     1921 taagatgaaa tgatttcgta aatggtctct gcacacctct ctttttcgta tgggttgata
     1981 ctgtttgcag aaattgtctg atgtcccgga gataaaacat gctctatacc ttttgatatg
     2041 gattctctgt cattggcaca gtgaactaca ctttgggacg caatgcggcc tttttgacga
     2101 tcaccaatat tgagagtagg aattccaaat gaaggcactt caataattcc gcttgaagag
     2161 tttcctacga ccgccttgac atattgcagt gctgaaagat agcgttttac tcccagagaa
     2221 gtgaaggctt tggagctata tggatgttgt tttacatact cttcaatggc ttgcacaatc
     2281 ggttgatggc cggtgtcaga attgggcatt gtgaatatca ctctgagctc aggatattgc
     2341 gaaacagctg cgagtaactc agcaatacct gttttggaat caccttctag agtctcagga
     2401 tgataggtga caaggattgt gcggtcattg aatttgaaat taagagagct ctcaagctct
     2461 tctttgccca tcagtggaat ttgttttata ttctccactc cgatagagcc tacattaaag
     2521 actctttccg gattttcacc gagctgtatg acacgttttc gatactcttc acatgaagtg
     2581 aaatgaagtg aactcatttt tgtgatagag tgacggattg catcgtcata agctccttca
     2641 gttacctctc ctccatgaag atgagctatc ggaatcctgg aaatcagtgc tgcggtggca
     2701 gctgcaagca tttcatgtct gtcacctaga atgatgatta tatcaggttt tagttccgaa
     2761 taggctattc caaatccctc cagtcccctt gacattgcca ttaatgtgga aacaggcgag
     2821 tcttcagcct gttcatcaat aattggaacc cttttatcaa ttttaaatcc atcactctcg
     2881 atttcccgat atgtttcacc aaactttggg gatagatgca tattagtggc aataatctgc
     2941 aattgcaagg ttgaatcccc ctgcacaagg cgcataaggc gacttaaaag gccatactcg
     3001 gcacgggttc ctgttacaaa gcagattttt ctcatatatc aatcaattgg tcttctacaa
     3061 aatctctctt agctatttgt cctataactt catcccatct cattggagag ataccatttc
     3121 caggtcgttt tacggtgaga ttttcttctg taaacaattc acctttcttt atttgacggg
     3181 aagccacaat acttttgcgt gcagcctcca gattctttct ttctgattca gtaatgctct
     3241 tatggcctgt accaaacgcc agctcgatat tacggatggc ggttaccatt gctttcaatt
     3301 catccggctc tagggaggct ttatggtcgg gaccctccat tttcttatca agagtaaaat
     3361 gtttctcgat aaccattgct cccagtgcaa cagctgcaat agggacctct atgcccattg
     3421 tgtggtctga ataacctatt ttaacaccga atttatcacc aattgtaagc atagccttaa
     3481 gatttacatc cttaaatggt gtaggatatt ctgtgttaca atgaagcacg gtaatctgat
     3541 caa
//
LOCUS       DAOSIE010000147         3527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_23261_length_3527_cov_3.402650, whole genome shotgun sequence.
ACCESSION   DAOSIE010000147 DAOSIE010000000
VERSION     DAOSIE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23261_length_3527_cov_3.402650"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="PLI69_05030"
     CDS             complement(<1..1505)
                     /locus_tag="PLI69_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG41887.1"
                     /translation="MIPELLKNIIRTYSEEDASMVTRAYRIAESELKERERGNNKPFI
                     EHPLGVVSIISEMGLLADSVTAVLLHEANRFNAASPSELNKTQLFNSFKSRFPKEVVD
                     IVSGLNNIASIKLQETNLDAERYRKLIVSYSSDPRVILIKLADRVEVMRNLDILPASK
                     HKSKMMETMLLYIPLAHQLGLYHIKSELEDLFLKYAEPEQYRAITNNLKATKRDRDLL
                     VSRFVNPLRDKLSRSGIEYTLKARTKSAYSIWKKMQAQKVPFEKIHDVFAIRFIIESP
                     PDREKEQALCWQVYSLVTEKYQTDTSRLRDWITVPKENGYESLHTTVQYKEGVTIEVQ
                     IRTARMDHEAEHGHASHWSYKGIKSESGITGWLDRVRMIMQDDNDMRQYTQVSPILLN
                     EIFVFTPTGDLRQLSEGATVLDFAFDIHTNLGLKCSGGRVNGKMVPIREKLKTGDVVE
                     IISNKNQKPTADWLNIAVTSKARSRIKQKLKEEENKLASAGKEIFERRLKN"
     gene            complement(1515..3506)
                     /gene="uvrB"
                     /locus_tag="PLI69_05035"
     CDS             complement(1515..3506)
                     /gene="uvrB"
                     /locus_tag="PLI69_05035"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOG41888.1"
                     /translation="MEFRLSSSYKPTGDQPEAIEQICNSIRGGATATTLMGVTGSGKT
                     FTMANVIERLQRPTLILSHNKTLAAQLYAEFKSFFPDNAVEYFVSYYDYYQPEAYLPT
                     TDTYIEKDLSINDEIEKLRLSTSSSLLSGRRDIVVVSSVSCLYGIGNPEDFHSTTIRI
                     KRGDLFSRQQLLYKLVDALYSRNEIEFNRGNFRVKGESVDIYPAYGDISFRILFFGDE
                     IESIEMTDPISGATLEYLDEISIFPANNFVTTKERIRSAIHNIQDDMMAQYNYFREIG
                     KHLEAKRLKSRVEYDLEMIKEIGYCPGIENYSRYFDGRKAGDRPFCLLDYFQDDFLTF
                     VDESHVTLPQVRAMYGGDRSRKETLIEYGFRLPAAADNRPLKFDEFESIIGQRLFVSA
                     TPGEYELEASGGVIVEQIVRPTGLLDPPIEIRPIANQVDDLLEEIEIRAEQDERVLVT
                     TLTKRMAEELEKYLTKASVRARYIHSDVDTLERVEIIEDFKEGRFDVLAGVNLLREGL
                     DIPSVSLVAILDADKEGFLRSGRALTQTAGRAARNLNGKVIMYADTITRSMKETMDET
                     ERRRAKQEAYNKANNITPTQVAVSARSVLSRSTYQAEEETKVRMLSEDPIISRMSPAE
                     LVRAIDATKKKMEDAAAELDFIMAAKYRDEMNSLKKLLK"
BASE COUNT          833 a          836 c          779 g         1079 t
ORIGIN      
        1 cagtttttaa gccttctttc gaaaatttct tttccggcgg atgcaagctt gttttcctcc
       61 tcctttagct tctgtttgat tctactccgg gcttttgatg taacggcgat attgagccag
      121 tctgcagtag gtttttggtt tttgttgctt atgatttcca ctacatcacc ggtttttagt
      181 ttctccctga tagggaccat tttaccgttg acacggcctc cggaacactt taacccgagg
      241 ttagtgtgaa tatcaaatgc aaaatccaat accgttgcac cttctgaaag ttgtctcagg
      301 tcgccggttg gggtaaagac aaatatttcg ttgagcagga taggggagac ttgtgtgtat
      361 tgtctcatgt cattgtcatc ctgcataatc attcgcactc tgtcaagcca accggttata
      421 ccgctctcgc ttttgatgcc cttgtatgac cagtgtgatg catgaccgtg ctccgcctcg
      481 tgatccatcc tggcagtacg tatctgaact tcaattgtga ccccctcctt atattggacg
      541 gtggtatgaa gggattcgta accattttcc ttgggtacgg taatccagtc gcgaaggcgt
      601 gaggtgtcag tctggtattt ttcggttaca agggaataga cttgccaaca taatgcctgc
      661 tccttttccc tatcaggggg agattctatt ataaatctta tggcaaatac atcatgtatt
      721 ttctcaaagg gtactttctg cgcctgcatc tttttccaaa tggaatatgc actcttagta
      781 cgtgccttga gcgtgtattc gatgccgctg cgactgagtt tatctctcag agggttgaca
      841 aagcgactaa ccagcagatc acgatccctt ttggtggcct tgaggttgtt ggttattgca
      901 cgatactgct caggttccgc atattttaga aacaggtctt ccagttcgct tttaatatgg
      961 tagagtccta gttgatgtgc cagaggtata tagagcagca ttgtctccat catcttggac
     1021 ttgtgctttg aggccggtaa aatgtcgaga ttacgcatta cctctacacg atccgcaagt
     1081 ttgataagga ttacccgggg atcgcttgag taagagacaa tgagttttcg atagcgctcg
     1141 gcatccagat ttgtctcctg tagcttaatt gaagctatgt tattcagtcc ggagacaata
     1201 tccacaacct ctttcgggaa acgggatttg aaactattga atagttgggt tttgttgagc
     1261 tcggatgggc ttgccgcatt aaaacggttg gcttcatgta aaagaacggc tgttacggaa
     1321 tctgccaaaa ggcccatttc cgaaatgatt gatactactc ccagtgggtg ttcgataaag
     1381 ggtttgttat tcccgcgttc tctctccttc agttcggatt cagctatgcg ataggcacgt
     1441 gttaccatcg aagcatcctc ttcgctatag gtacgaatta tatttttaag caattccgga
     1501 atcattgtct tctgctattt taataatttt ttgagggaat tcatctcatc cctatatttt
     1561 gcagccataa tgaaatccaa ttcggccgca gcatcctcca ttttcttttt ggttgcatct
     1621 atggcacgaa ccagttctgc aggagacata cggctgatta tgggatcttc tgaaagcatc
     1681 ctcacttttg tctcttcttc agcctgatat gtgctcctgg agaggacact cctggcagag
     1741 accgccactt gagttggagt gatattgtta gccttgttgt aagcctcctg ttttgccctg
     1801 cgcctctcag tctcatccat tgtttctttc atggagcgag tgatggtatc tgcatacatt
     1861 atcacctttc cgttgagatt gcgcgctgct ctgccggccg tttgtgtaag agctctcccg
     1921 gagcggagaa atccctcctt atcggcatcc agtatcgcca ccaatgagac cgaaggtatg
     1981 tcgagacctt cacgcaacag attgactcct gccaggacat caaacctgcc ctccttgaaa
     2041 tcctcaataa tttccactct ctcaagtgta tccacatccg agtgaatgta gcgtgccctt
     2101 acggaggcct ttgtaaggta tttctcaagt tcctctgcca tcctcttagt caaagtagtg
     2161 acaagcactc tttcatcctg ttccgcacgt atctctatct cctccagcaa gtcgtctacc
     2221 tggttggcta tgggccggat ttcaatcggc gggtccaaaa gtccggtagg acgaactatc
     2281 tgctccacaa ttactccccc agaggcttcc aattcatatt ctccgggggt tgcactcaca
     2341 aagagccgtt ggcctataat tgactcaaat tcatcaaatt tcagcgggcg gttgtcggca
     2401 gcggcaggaa gacggaatcc gtactcaata agagtctcct tacgagaacg atctccacca
     2461 tacattgccc ttacctgagg tagtgtcacg tgactttcat ctacaaaggt aaggaaatca
     2521 tcctggaaat agtctaacag gcagaagggt cggtcacctg ccttacggcc gtcaaaatat
     2581 ctcgaataat tctcgatccc gggacaatag cctatttcct tgatcatttc caggtcatat
     2641 tccacacgac tttttagcct tttggcctca agatgtttgc caatctccct aaaatagttg
     2701 tattgggcca tcatatcatc ctgaatattg tgaattgcac tgcgaatgcg ttctttagtc
     2761 gtgacgaagt tgtttgcagg gaatatggat atctcatcca aatactccag cgtggctccg
     2821 ctaatgggat ctgtcatctc tatgctctca atctcatctc caaaaaatag tatcctgaaa
     2881 gatatatcgc cataagccgg ataaatatcg acagactctc cctttaccct aaagttgccc
     2941 ctgttgaatt caatctcatt gcgcgaatat agagcatcaa ccaacttata aagtagctgt
     3001 tgacggctaa ataaatcccc ccttttgata cggatggttg tactgtgaaa gtcctccgga
     3061 ttgccaatgc cataaagaca ggataccgag gagaccacaa caatgtccct tcgtccggaa
     3121 aggagagatg aggaggtgct aaggcggagc ttttctattt catcattgat acttagatct
     3181 ttctcgatat aggtatcagt tgtgggcaag taggcctccg gttggtaata atcgtagtat
     3241 gaaacgaaat actctaccgc attgtcggga aagaaggatt tgaactcagc gtacagctgt
     3301 gcagcgaggg tcttgttgtg gctcaggata agcgtaggtc tctgaaggcg ttctatgacg
     3361 ttggccatag tgaaggtttt gcctgatcct gtcacgccca taagcgttgt ggccgtagca
     3421 cctcctctga tcgaattaca gatctgctct atggcttccg gctgatcacc tgtcggttta
     3481 tatgacgaac ttagcctgaa ttccattatt ggcctctatt tcctttt
//
LOCUS       DAOSIE010000148         3470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_23711_length_3470_cov_3.171596, whole genome shotgun sequence.
ACCESSION   DAOSIE010000148 DAOSIE010000000
VERSION     DAOSIE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23711_length_3470_cov_3.171596"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..949
                     /locus_tag="PLI69_05040"
                     /pseudo
     CDS             1..949
                     /locus_tag="PLI69_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331427.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
     gene            1074..1688
                     /locus_tag="PLI69_05045"
     CDS             1074..1688
                     /locus_tag="PLI69_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41889.1"
                     /translation="MQNKLQELADKLYNEGLSKGKQDAEELKAKALREAEKIVSKAKE
                     EAAAIVEKAEKEATEIITRADNDVKMASNQAINAVRQQVEKMIITNTITPAVKEAMSD
                     TKLIKSVITTIAGAFNAANPDSIALEMIVPQSLKSELEDFLKKEINGKLCKGLEVKYV
                     KGLSSGFKIGPKDGGYMISFTDEDFSAMIGGYLRPSTRRILFGE"
     assembly_gap    1819..1828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1829..2247
                     /locus_tag="PLI69_05050"
     CDS             <1829..2247
                     /locus_tag="PLI69_05050"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOG41890.1"
                     /translation="FGLDMEKLSRHFYRAAAQHPNLFIREFFSFDKSLRDARICWLDN
                     VVYEGDFEEYPRLKQIFAMDNLIERERRLDNLIWDKINEISSKELFNINILLAFFTKA
                     RIVERWTRLDPESGRELFARLVNEVRGTFKGVNYKS"
     gene            2271..>3470
                     /locus_tag="PLI69_05055"
     CDS             2271..>3470
                     /locus_tag="PLI69_05055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOG41891.1"
                     /translation="MKSTGIVTGIISNLVTVEVDGHVSQNEICYIDLGGTQLMAEVIK
                     ISGKNAFVQVYESTRGLKQGDKVEFVGHMLEATLGPGLLSSSYDGLQNNLETMEGVFL
                     KRGEYTKPLDHDRVWHFTPCVNPGDKVFASDWLGEVMEGWLHHKIMVPFTFEGEFTIK
                     SIAPEGDYKIDDTIALLIDSQGEEYKVTMVQKWPVKVAIGGYIDKPRPYRIMETGVRC
                     MDTLNPIAEGGTGFIPGPFGCGKTVLQHAIARQGDAEVIVMAACGERANEVVEIFTEF
                     PKLIDPHTGRKLMERTTIICNTSNMPVAAREASVYTAMTVCEYYRAMGMKVLLLADST
                     SRWAQALREMSNRMEELPGADAFPVDLTAIISNFYSRAGIVNLRNGETGSITFIGTVS
                     PAGGNLKE"
BASE COUNT          985 a          776 c          803 g          896 t
ORIGIN      
        1 gcttgagtgt cggagtttat gaagagggat cagtattgaa cattctctac caggagttca
       61 gagaggtttc tacccgcctc tgattttcat gggaataggg gcaatgaccg acttctctgc
      121 gttgatatcc aaccccaaac tacttcttat cggtgctgct gcacagtttg gtatcttcgg
      181 agcttatgcc atctctctcg ctgttgggtt tgaccctatg caagcgggcg ctattggtat
      241 cataggtggt gctgacggtc cgacagccat cttcctgaca tcaagactgg ctcctgacct
      301 tttgggtgca gttgcagttt cagcctattc atatatggcc ttggtacccg ttatacagcc
      361 acctatcatg aaagttctca ccacaaagaa ggagcgtctg atccgtatga aaccccctag
      421 agcggtatct cctactgaga agaaactctt tccaatcatc ggttttttga taacggcatt
      481 catagtacct tcaggtattc cgttactcgg tatgctattt ttcggtaacc ttatcaagga
      541 aagtggtgtt actaaacgtc tcgcaaacac agcaagcggg cctatggttg acgtggtaac
      601 catacttata ggtcttacag ttggagcttc gactcaggca gatcatttcc tcagacccga
      661 ctctgtaaag attttcgcca ttggtgcatt atcatttatg attgcaacat ttgcgggtgt
      721 tctctttgta aaaatcttca acctcttcct caaagaaggt aacaagatca accctcttat
      781 cggtaatgcc ggagtatcgg cagtgcctga ctcagccagg gtgtcccaac ttgttggtct
      841 cgaatacgac aaaacaaatc acctgttgat gcacgccatg ggacccaacg tggccggcgt
      901 catcggcagt gctgttgcag caggtatttt acttggattc ttggcataat ttcagacaaa
      961 ttctgaataa aaaagacggt aaaagaaaaa tttaccgtct tttttgttgc tattgctttt
     1021 caatttattt atatttgtcg cttgaaacat taagtattta taaatcttac gatatgcaaa
     1081 acaagttgca ggaacttgcc gacaagctct ataacgaagg gctgtccaaa ggaaaacagg
     1141 atgctgaaga gctaaaagcc aaggcactcc gtgaagcaga aaaaatagta tctaaggcaa
     1201 aggaagaagc tgccgccatt gtcgaaaaag ctgaaaaaga ggcaacagag attataactc
     1261 gtgccgacaa tgatgtaaaa atggcctcta accaagccat taatgcagta agacagcaag
     1321 ttgagaagat gattatcaca aataccatca ctccggctgt caaagaagcc atgtccgata
     1381 caaaactgat caaatccgtt attaccacta tcgcaggtgc atttaatgct gcaaaccctg
     1441 actcaattgc tctagaaatg atagtacctc agagcttgaa gtcggagctt gaggattttc
     1501 ttaaaaagga aatcaacggg aagctctgca agggacttga agtgaaatat gttaaagggc
     1561 tttcttcagg atttaaaatc gggcctaaag atggaggcta catgataagc tttactgacg
     1621 aagactttag tgcaatgatc ggcggatatc tcagaccctc taccagacga attttatttg
     1681 gcgaataatg ggcaattatc actatataat cgcaggattg cccgatcttc tgctcgacta
     1741 tggttcccat gatctagatg ccgcctcact tcagaagagc gttaccgaaa tgctttcaga
     1801 tgaggaccga aggatcatnn nnnnnnnnac ttcggtctgg acatggagaa actctctcgt
     1861 catttttacc gtgccgcggc acaacatccc aacctcttta taagggaatt cttttccttt
     1921 gacaaatctc taagagatgc ccgcatctgc tggctcgaca atgtagtcta cgaaggcgat
     1981 ttcgaggaat accctcgtct gaaacagatt ttcgcaatgg acaacctcat agagagagaa
     2041 cgcagacttg acaatttaat ttgggacaaa atcaatgaaa tatcctccaa ggaacttttc
     2101 aatattaata tcctgcttgc cttcttcact aaggcacgga ttgttgagag atggacgcga
     2161 ctcgatcccg aaagcggcag agaactcttt gcacgtcttg taaacgaggt aagaggtact
     2221 tttaagggag tcaattataa atcataaata agaataatat acatacaagc atgaaaagta
     2281 caggtattgt aacaggcatc atttccaacc tggtgacggt cgaagttgat ggacatgtct
     2341 cccagaatga gatttgctat atcgatctcg gaggcaccca acttatggcc gaagtgatca
     2401 aaatctccgg aaagaatgcc tttgtacagg tatacgagag cacaagaggt ctaaaacaag
     2461 gagataaagt cgagtttgtc ggccatatgc tcgaagccac cctgggtccc ggacttctct
     2521 ccagcagtta tgatggcctt cagaataacc tcgaaaccat ggagggggtt ttccttaaga
     2581 gaggagaata caccaaacct ctggaccacg acagggtatg gcactttaca ccttgcgtaa
     2641 atccgggtga caaggtgttt gcctccgact ggctcggaga ggtaatggag ggctggctgc
     2701 accacaaaat tatggttccc ttcacctttg agggtgaatt taccataaag agcattgctc
     2761 cggaagggga ctataagata gatgatacaa tagcactttt aattgactcc cagggggaag
     2821 aatacaaagt gacaatggtt cagaagtggc cggttaaagt tgccatcgga ggttatattg
     2881 ataagccccg tccctatcgc atcatggaga ccggagttcg ttgcatggat accctcaacc
     2941 ccattgccga gggtggcaca ggctttattc cgggtccatt cggttgcgga aagactgtac
     3001 tacaacatgc aatcgcccgt cagggagatg ccgaagttat agttatggct gcctgcgggg
     3061 agcgagccaa tgaagtggtg gagatcttca ccgaattccc gaagctgata gaccctcata
     3121 ccggccgcaa gctcatggag aggaccacaa tcatttgtaa tacatccaac atgccggtag
     3181 cggcacgtga agcttcggta tataccgcta tgaccgtatg tgaatattac cgcgcaatgg
     3241 gtatgaaggt actcctgctt gcagactcaa cctccagatg ggctcaggcg cttcgagaaa
     3301 tgtccaaccg tatggaggag ctgccgggtg ctgatgcatt cccggtggac cttactgcaa
     3361 ttatatccaa cttctattcg agagcgggca tagttaatct ccgtaacggc gagacaggct
     3421 caataacttt catcggtacg gtatcccctg ccggtggaaa cctgaaagaa
//
LOCUS       DAOSIE010000149         3463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_23785_length_3463_cov_4.606221, whole genome shotgun sequence.
ACCESSION   DAOSIE010000149 DAOSIE010000000
VERSION     DAOSIE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23785_length_3463_cov_4.606221"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="PLI69_05060"
     CDS             <1..1285
                     /locus_tag="PLI69_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOG41892.1"
                     /translation="VPASALLVSATYTAVALILTVVFLVAAIVPGYLYSRIPAQSAIR
                     NYSENKRIWKLSLLFVQTAICALLLSLVLVINKQYTKAIHDKPGYEYENLLWTVLTGT
                     DKKVHQGIVDELKTLPEVLGVEMSYGLPLDSGSGNNVIDGDKHLFNIADQYEGTAGLF
                     DLLGIPFIEGKYPQSPLEVAVSESFLTEMAKFEDWSDGAVGKQIFVTEHSLTPTHSFT
                     ISGVYKDYRMGTLTGQDSRASVKFLGEIGEDWMPFLAIKVNEVSRELIDKVQNIIQSR
                     IENRVVEVKAYKDSMREAYSGERRTKNTVIIGCVVSIVIALFGLIGYIRDESQRRSKE
                     MAIRKINGASVKEIITIYIKEVLKIGIVALILGNAGAYYAAHLWLQNFSEKITLSPWY
                     FIAADIIIIILIITTIVLNSLRISKANPVESLKNE"
     gene            1313..1993
                     /locus_tag="PLI69_05065"
     CDS             1313..1993
                     /locus_tag="PLI69_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG41893.1"
                     /translation="MITVKDLSKVFRTEEIETTALNKVSFNIDDGEFVAVMGPSGCGK
                     STLLNILGLLDNPTEGSYIFAGQEVAKLKEKDRTKFRKGNIGFVFQSFNLIDELDVYE
                     NVELPLKYMSISASERKKRVTEILKRMNISHRAKHFPQQLSGGQQQRVAIARAVVANP
                     KLILADEPTGNLDSKNGKEVMDLLSELHSEGTTIVMVTHSQKDAAVAQRIINLFDGQI
                     VSDVKNEL"
     gene            2074..>3463
                     /locus_tag="PLI69_05070"
     CDS             2074..>3463
                     /locus_tag="PLI69_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG41894.1"
                     /translation="MSKINIFRRGRGLSLSSVLNVLGMAVAFAAFYIILCQVNYEFGF
                     NKKIKDVDKVFVISSGEHAGGGKRNLSFCRPLAELIIDSSPNIEYGGIINLGYNGSGK
                     QSFWVDRNNEKHVVEMSATPISGDALKAIGLEPIAGSFDKMVNRTSFAISQSVAKKFD
                     LEVGMQLNQGDTPGTIVAIYEDMAENSHLKHCELLYNLDKENLDSYSEWGYTYFVKLI
                     DPSQKEDMEKNLTSTIRPFFKDKLLDQIAEAGEEIDASDEEINKAINTFLDMADVQLI
                     PLGGLYFQEGISSMIDFGNRTTTTVLLIVAILVIGIAFVNYINFFFALIPERIRGVNT
                     KKVLGCSRRELIMSVVTESLTLIIVALILAYAFVMLFNMSELVHLVAAKTALSANISL
                     AISTVIIALVVAVVSSLYPAFYITSFEPALVLKGSFGATKQGKTLRYGLVGLQFVISI
                     GLIIVSSFINLQR"
BASE COUNT         1045 a          660 c          792 g          966 t
ORIGIN      
        1 cgttccggca tctgcactcc ttgtaagtgc tacatatacg gcagtagctt tgattctcac
       61 agtagttttc ttagtggcag caatagttcc aggctatctc tattcaagaa ttccggcaca
      121 atctgcgatc agaaattata gcgagaataa gagaatctgg aaactctcgc ttctctttgt
      181 tcagacagca atatgtgctc tgcttctctc tttggtgctg gtaatcaaca agcaatatac
      241 caaagccatc catgacaaac ccgggtatga gtatgaaaat ctgctttgga cggtgctcac
      301 aggtacggac aaaaaggttc atcaaggtat tgttgatgag ctaaaaaccc tgcctgaggt
      361 attaggcgtc gaaatgtcat acggtctgcc tttggatagc ggttcaggaa ataatgtgat
      421 tgatggtgat aagcatttgt tcaatattgc agaccaatat gaaggtacag ccggtttgtt
      481 tgatctgttg ggaattccat tcatagaggg aaagtatccg caaagtccct tggaagttgc
      541 tgtaagtgag agttttttaa ctgaaatggc aaaattcgag gattggagtg acggcgctgt
      601 aggtaagcaa atatttgtta ccgaacacag cctgacacct acacattcct tcactatatc
      661 gggagtctat aaggattata gaatgggcac attaacaggt caggatagta gagccagtgt
      721 taaatttctc ggcgagattg gtgaagattg gatgccgttt ttggcgataa aagtgaatga
      781 agtttcgaga gaattgatcg ataaggtgca aaatattatt caatcccgaa tcgagaacag
      841 agtggttgag gtaaaggcct ataaagactc aatgcgagag gcctattccg gtgaaagaag
      901 aaccaaaaac acagttatca tcggttgtgt tgtctctatt gtcatcgcac tctttggcct
      961 tatcggctat atcagagatg aatctcaacg cagaagcaaa gagatggcca tcaggaagat
     1021 aaacggcgcc agcgtaaaag agattataac catttatata aaagaagttc tgaaaatcgg
     1081 tatagtcgcc ctaattctcg gcaatgcagg agcctattat gcggctcatt tgtggctaca
     1141 gaatttctcc gaaaaaatta ccctttcccc ttggtacttc atagcagcgg acatcatcat
     1201 tatcatcttg attattacaa caatcgttct gaatagcctt cgtatatcca aggctaatcc
     1261 ggtcgagtcg ttaaagaatg aataaataaa caattaaata aataacacaa ttatgattac
     1321 agttaaagat ctcagtaaag ttttccgcac ggaggaaatc gagacaacag cattaaacaa
     1381 ggtttcattt aatatcgacg atggcgaatt tgtagccgtt atgggacctt cgggttgcgg
     1441 taaatcaacc cttttaaata ttcttggttt gctcgataac ccaacggagg gcagttacat
     1501 ttttgccggc caggaagtag ccaaattgaa agaaaaagat agaaccaaat tccgtaaagg
     1561 aaacatcggt ttcgtatttc agagctttaa ccttatagat gagctggatg tatatgaaaa
     1621 tgtggagctt ccacttaaat atatgagcat cagcgcatcc gaacgcaaga aaagagtgac
     1681 ggaaatcctc aaaagaatga atatcagcca tagagcgaaa catttccccc agcaactatc
     1741 cggcggccag caacagcgtg ttgccattgc ccgcgccgtg gtggccaacc ccaagctgat
     1801 ccttgcagac gagccgacag gtaatcttga ttcaaagaat ggtaaggaag tgatggatct
     1861 gcttagcgaa ctccattccg aaggcacaac catagtaatg gtgactcact cccaaaaaga
     1921 cgcagcagtt gctcagcgta tcatcaatct gtttgacggt cagatcgtaa gtgacgttaa
     1981 aaacgaattg taactgcaaa gaccctcaca tcaccttacg ggctcctcag ggtgatattc
     2041 taaggggtgc aaaaagtgtt aattaataag aatatgagta aaataaatat atttagacga
     2101 ggtcgggggc tatctctctc cagtgtattg aatgtgctgg gtatggcggt tgcctttgct
     2161 gccttctaca taatactctg tcaggtaaat tacgagtttg gattcaacaa aaagattaaa
     2221 gatgttgaca aagtctttgt gatttcttca ggagaacatg ccggcggagg gaaaagaaat
     2281 ctctcattct gtcgtccttt ggcggagtta atcatcgatt cctcacccaa catagagtat
     2341 ggcggaatta tcaaccttgg atataatgga agtggaaagc aaagtttctg ggtggatcgg
     2401 aacaatgaga agcatgtagt tgaaatgagt gccaccccta tcagcgggga tgctctcaag
     2461 gctattggat tggagccgat tgcaggttca tttgataaaa tggttaacag gaccagcttt
     2521 gctataagcc aaagtgtagc gaaaaaattt gatttggagg tagggatgca attgaatcaa
     2581 ggggatactc ccggaacgat tgtggcaatt tatgaagata tggctgaaaa ctcccattta
     2641 aagcactgtg agcttctcta caacctcgat aaagagaacc tggatagcta ttcggagtgg
     2701 ggttacacct actttgtaaa actcatagat ccttcgcaga aggaggatat ggaaaaaaat
     2761 ctcacctcta ctattcgtcc tttctttaag gataagcttt tagatcaaat agctgaagct
     2821 ggagaagaga ttgatgcctc ggatgaagag ataaataaag cgataaacac ctttttggat
     2881 atggctgatg ttcagctaat acctttggga ggcttatatt ttcaagaagg catatcgagt
     2941 atgatagatt ttggaaatcg tacgacaact acggtacttc tgattgttgc gatattggtg
     3001 ataggaatcg catttgtaaa ctatatcaat ttcttctttg ccttaattcc ggagcgcata
     3061 agaggtgtca atactaagaa ggtgctcggc tgttcccgca gagagcttat tatgagcgta
     3121 gttactgaga gtttaacact gataatcgta gcgttgatat tggcatatgc ctttgtaatg
     3181 ctctttaata tgagtgagtt ggttcatcta gtggctgcaa aaaccgcttt aagtgcgaat
     3241 ataagcctcg cgatttcaac agttatcatc gctcttgtgg tagccgtagt ttccagcctc
     3301 tatcccgctt tctacattac ttcatttgag ccggctctgg tattaaaagg ctctttcgga
     3361 gccacgaaac agggaaaaac cttgcgttac ggacttgtag ggctacagtt tgttatctct
     3421 atagggttga ttattgtctc ctcttttatc aatctgcagc gca
//
LOCUS       DAOSIE010000150         3449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_23891_length_3449_cov_5.576606, whole genome shotgun sequence.
ACCESSION   DAOSIE010000150 DAOSIE010000000
VERSION     DAOSIE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23891_length_3449_cov_5.576606"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PLI69_05075"
     CDS             <1..1072
                     /locus_tag="PLI69_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG41895.1"
                     /translation="IVSSFINLQRNYMVNYDMGFDKEQLLYVHTTYDIGKNAEAVEER
                     LKSDPQIQDVTWASGELIAPIRMGWGRSFKGEAITFQCYPVAWDFPEFMGIEILEGRS
                     FRKEDEIGDNGVFIFNEACKLKFGLTLDDKINGHLSEATDIVGFCNSFSFMTLKEEVG
                     PFALYVYGKSPWTYPSTAFIRVAVNANYQDVMKHIGTVLSEWAPKIAPDQWTINFFDE
                     NIEKTYQKEKSLSQLISLFTLIAIVISLMGVFGLVMFETQYRRREIALRRVSGASIKD
                     ILIMFCSRFVKIVLICFVVAAPISYYIVSQYLKTFAHRISIYWWVFALALIAVMFVTL
                     CVVVIQTWKAATADPVDSIKTE"
     gene            1147..2493
                     /locus_tag="PLI69_05080"
     CDS             1147..2493
                     /locus_tag="PLI69_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG41896.1"
                     /translation="MAKNKAKILVVDDNGGIRAALKLLLPIHFSEVELISSPKELMSK
                     ISSFSPDVVLLDMNFHTDINTGNEGLFWLSEIKSKYEEIEVVLFTAYADIALAVEGMK
                     RGAFDFIVKPWDNEKLIDTLRKASLVNKKEPKPTESEADSSMFWGNGKAMKAIRSRVN
                     KIADTDATILITGENGTGKDMLAREIHRLSDRAQKPIVNVDAGAITETLFESELFGHV
                     KGAFTDAHADHVGKFELADGGTLFLDEIGNIPLHLQAKLLRVIQNRNVVRVGDTKEIP
                     IDIRLICATNKDLEKMVAGEEFREDLYYRINTIHLHLPPLRERIDEIEPLAKLFLGIF
                     AKKYRRNVSEISEEVLELLKSHPWSGNIRELRNCIEKAVILSEGAILTPKEIELKSIQ
                     SDSGNREISSEDSLEETEKKAIMAAIDRFGGNLSMVAKSLGISRPTLYAKLKKYNI"
     gene            2490..>3449
                     /locus_tag="PLI69_05085"
     CDS             2490..>3449
                     /locus_tag="PLI69_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG41897.1"
                     /translation="MTTWQVILLILGVIVLTAIITSYLTSRRMRRKVSYMLDALEDKE
                     YNFHFGEKGFFAKRFNVSLNRLRNLFDQERKEISAQEKYYGLMLDHVKTGVIVIEQNG
                     RVNYSNKTALELLGVATLGNIKQLKTVSDSLYEAFEAITDTGLEQRANFYNESGKVTI
                     ALTSSKATIGQKEVEVVAFNDITSNISESEQESWAKLIRVLTHEIMNTVTPIASLSEA
                     LQQFDNVEEQVRSGLETIAQSSRGLIKFVDTYRTLTRVATPVKKAVLVRDLVERVISL
                     TQEQAQNWGAAVIFEEKSEDILLYADEGQIIQILINIVKNALEA"
BASE COUNT         1043 a          640 c          802 g          964 t
ORIGIN      
        1 tattgtctcc tcatttatca atctgcagcg caattatatg gtaaattatg atatgggctt
       61 tgataaagag caacttctct atgtacatac tacttatgat atcggaaaaa atgccgaggc
      121 tgttgaagag agattgaaga gcgatcctca gattcaagat gttacatggg cttcaggtga
      181 actcattgcc cctataagaa tgggatgggg acgcagtttt aaaggcgagg cgattacttt
      241 ccaatgctat ccggttgcat gggacttccc cgaatttatg ggcatagaga tattggaagg
      301 gcgttctttc cgtaaggagg atgaaattgg tgacaatgga gtctttattt tcaatgaagc
      361 atgtaagctt aaatttgggt tgactttgga tgataaaatt aacggacatc ttagcgaagc
      421 aacagacata gtaggtttct gtaatagttt cagctttatg acactcaagg aagaggtggg
      481 accctttgcg ctctatgttt atggaaaatc cccttggacc tatcccagca cagcatttat
      541 tcgtgtagca gtaaatgcaa attatcagga tgtaatgaag catatcggca ccgtacttag
      601 tgagtgggcc cctaaaattg ctcctgacca gtggacaatc aattttttcg acgaaaatat
      661 agagaaaacc tatcagaagg aaaagtcgct ttctcagttg atcagcctct ttactttgat
      721 tgctattgta atttctctga tgggtgtctt cggcctggta atgttcgaga cgcaatatcg
      781 ccgccgtgaa attgccctta ggagagtgag cggagcctcg ataaaagaca ttttaattat
      841 gttctgcagc cgttttgtga agattgtgtt gatctgtttt gtggtggctg cccctattag
      901 ttattatatt gtaagtcaat atcttaagac ttttgcgcat cgtatctcga tttattggtg
      961 ggtatttgct ctcgctttaa ttgcagtgat gtttgtaacc ctttgcgttg tagtaattca
     1021 aacctggaag gcagctacgg cagaccccgt tgactcaata aagacggaat aggattatgc
     1081 caattgccaa gaagtcacta catttgtagg gtcagataga tatgacttga aaatatagag
     1141 gattctatgg ctaaaaacaa ggcaaaaata ttggttgtcg atgacaacgg tggcatcagg
     1201 gccgctttga aactactttt accgattcat ttttctgagg tggaacttat ctcctctccg
     1261 aaagaattaa tgagtaagat ttcaagcttc agccctgatg tcgtgttgct cgacatgaac
     1321 tttcacaccg atatcaatac cggaaatgag ggacttttct ggctctctga gatcaaatca
     1381 aaatatgagg agatagaggt ggttctcttc actgcctacg ccgatattgc gctcgctgtg
     1441 gaagggatga aacggggtgc atttgatttc attgttaagc catgggacaa tgaaaagttg
     1501 attgacactc tccgtaaggc ctctcttgtc aataaaaaag agccgaagcc gaccgagtcc
     1561 gaagcagatt cttcaatgtt ttggggcaac ggcaaggcta tgaaggcaat tcgcagcagg
     1621 gtaaataaga tagctgacac agatgcaact attttgatca cgggagaaaa tggtaccggt
     1681 aaagatatgc ttgcccgcga aattcatcgc ctctcggata gagcacaaaa accgatagtc
     1741 aatgtagatg ccggagcaat aaccgaaacc ctttttgaaa gtgagttgtt tggtcatgtg
     1801 aaaggtgcct ttaccgatgc tcatgcagac catgtcggta aatttgagct tgctgatggc
     1861 ggtaccttgt ttttggatga aatagggaat attccccttc accttcaggc caaacttctg
     1921 agagtaatcc aaaacaggaa tgtagtgaga gttggagata cgaaagagat tcccattgat
     1981 atccgcctta tttgtgccac aaacaaggat ttggaaaaaa tggtagcggg agaagagttt
     2041 agggaagacc tctactatcg tataaataca attcatttgc atcttcctcc tttaagagaa
     2101 aggattgatg agatagagcc gctggccaaa cttttcctcg ggatctttgc gaagaaatat
     2161 agaagaaatg tctcggaaat aagcgaagag gtattggaac ttctaaaatc tcatccttgg
     2221 agcggcaata ttcgcgagct caggaattgt atcgaaaagg ctgtcattct ctccgaagga
     2281 gctattttaa ctcccaaaga gatagaactg aaaagtatcc aatccgattc ggggaataga
     2341 gagatcagct ctgaagactc tctggaagag acggaaaaga aagccattat ggcggctatt
     2401 gaccgtttcg gcggcaattt atctatggtt gcaaaatccc tcggcataag cagaccgaca
     2461 ctatatgcca aattgaaaaa atataatata tgacaacctg gcaagtcata ttgttgattc
     2521 ttggtgtaat tgttttaacc gcgattatta cttcttacct aacttcacgc aggatgcgaa
     2581 gaaaggtcag ttatatgctt gatgctcttg aggataaaga gtataatttt cattttggcg
     2641 agaaaggatt ttttgccaaa aggtttaatg tgagtctcaa tcgattgcgc aatcttttcg
     2701 accaggagag aaaagaaatc agtgctcagg agaagtatta cggtcttatg ctggaccatg
     2761 tcaaaacagg ggttatagtg attgaacaaa acggcagagt caattattcc aataaaacag
     2821 cgcttgaatt gctgggtgtt gctactctcg gcaatataaa acagcttaaa actgtcagtg
     2881 actccctgta tgaggctttt gaagctataa cagacaccgg tttggagcag agagcaaatt
     2941 tttacaacga atccggaaaa gtaaccattg ctctaacctc atcgaaggca accattggcc
     3001 aaaaagaggt cgaggtagtc gcctttaatg atatcacgag caatatttca gagagtgaac
     3061 aggagtcctg ggcaaagctg atcagagtgc tcactcacga aataatgaac acagtgacac
     3121 ccatcgcctc tttaagtgaa gctcttcagc aatttgacaa tgtggaggag caggttcgca
     3181 gcggattgga gactatcgct cagtcttcac gaggcttgat aaaatttgtt gatacttata
     3241 gaaccttgac aagagttgcc actcctgtca aaaaagcagt tttggtaagg gatttggtag
     3301 aaagagtaat ttccctgaca caagaacagg cacaaaactg gggagcagcc gttattttcg
     3361 aagaaaaatc cgaggatatc ctcctttatg cagatgaagg acaaataatt cagattctca
     3421 taaatattgt aaaaaatgct ctggaagct
//
LOCUS       DAOSIE010000151         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_24119_length_3423_cov_3.898456, whole genome shotgun sequence.
ACCESSION   DAOSIE010000151 DAOSIE010000000
VERSION     DAOSIE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24119_length_3423_cov_3.898456"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLI69_05090"
     CDS             <1..378
                     /locus_tag="PLI69_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG41898.1"
                     /translation="NIRKYDSNKASLKTWISRITVNLLIDHLRRNKKFTFVSIDEQRL
                     DIVEPEYNSLIHVPQDVILKMIADLPDSKRTVFNLYCLENYTHKEIAELLGIKENTSS
                     SILYKARVILADKVNAYIKEKGL"
     gene            375..1553
                     /locus_tag="PLI69_05095"
     CDS             375..1553
                     /locus_tag="PLI69_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41899.1"
                     /translation="MKTDFDKYISDQIQNIEDNLRVGNWGKFQTRYARHRGVKMVRII
                     IPVVAAASAIIVLLLSGINRIDNGIKRIDDNYLLIAENAPTIRTEVPAFRKVFMPSLR
                     HQEPTKPDTFHIPDSQPQIDAETLIQENVDAPKFEHATKKDSIHQPIPSYDYFDADND
                     ATAYIPKKSLSISSIGISGSQGLVGYSQFDMPFGAFQLRTSYAEEKPRGDISHEKPRD
                     SILVDKPDNIVLGETEEVKYRHLPPITVGVSLAINFTQRLVLITGLDYSLYLSEKKEV
                     ISPDKVRIERQQLHYLGLPFRLNYTIYSKKSFEWYAGGGFKVDKCIYAKSGAEVLKES
                     NFLLSALLTTGIQYNITRNFSIYCEPYCSYLFSDTGLPSYRSEKPFEISASLGIRVNL
                     "
     gene            complement(1583..2896)
                     /locus_tag="PLI69_05100"
     CDS             complement(1583..2896)
                     /locus_tag="PLI69_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOG41900.1"
                     /translation="MEQVKIILLFFIAALGLSACEKNDVPQVEDPIEIESPSPERMIT
                     KVTLTAEQKAYVGAGNAFAINSLKLLYAQEKESVIFSPLSLQYALAITANGASGETAS
                     EIIGTLGYGKDQKALNEYCNLLLNQLPALDDSVEVKLTDAVIVNKDFRVQEDFRNIAE
                     KVYYAPVEYIDATNPKVVVDRINEWAYRSTNGFIFPFLQESDIIPNFAAAILNALYFK
                     AKWSEESGKPMFEKESTKKSQPFYLDGGGERKVDYMVTSRSLRYGKIGKNAIVELPYA
                     HGKFAMYIILPDLKGGNGIERLFSSFTSENFSGALSSMSNDAVVNLRLPKFESENKYE
                     LTKMLNILGIRRAFDPNRAEFDKLLSHSAIKDFYIGSVIQKARINVAEWGTEAAAVTA
                     VQIGFTSIPNQPDKEIDFFADHPFVYGIVEKSSGVILFAGVYDGK"
     gene            complement(3181..3257)
                     /locus_tag="PLI69_05105"
     tRNA            complement(3181..3257)
                     /locus_tag="PLI69_05105"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3221..3223),aa:Ala,seq:tgc)
     gene            complement(3261..3337)
                     /locus_tag="PLI69_05110"
     tRNA            complement(3261..3337)
                     /locus_tag="PLI69_05110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3301..3303),aa:Ile,seq:gat)
BASE COUNT         1064 a          699 c          685 g          975 t
ORIGIN      
        1 aacattcgta agtatgattc caacaaggca tcgcttaaaa cttggatatc gcgtattacg
       61 gttaatctct tgattgatca tttaaggcgt aataagaaat ttactttcgt atcaatagac
      121 gaacagagat tagatattgt ggaaccggag tataatagtc tgattcatgt ccctcaagat
      181 gtcatattga aaatgattgc ggatttgccg gatagtaaaa ggactgtgtt taatttatat
      241 tgtctggaaa actataccca caaagagata gctgagttat tgggaataaa ggaaaatacg
      301 tcatcatcga tcctctataa agcgagagtt atattggcag ataaggtaaa tgcatatatt
      361 aaggagaaag gcttatgaaa accgacttcg acaaatacat ctccgatcag atacaaaaca
      421 ttgaagataa ccttcgagtt ggtaactggg gaaaattcca aacgcgctat gcacgacata
      481 gaggtgttaa gatggtgcgt atcataatac ccgtagttgc cgctgcctcc gctataattg
      541 tgttgttgtt atccggaata aataggatag ataatggaat aaaacggata gacgacaact
      601 atctcttgat tgccgaaaat gctccaacca tcaggacaga ggtccctgct tttaggaaag
      661 tatttatgcc ttcacttaga catcaggagc ctacaaagcc tgacaccttt catataccag
      721 attcccaacc gcagattgat gcggagacct taattcaaga gaatgtcgac gcacccaagt
      781 ttgaacatgc gacaaaaaag gattccatac atcaaccaat tccatcctac gactattttg
      841 atgctgataa tgatgctaca gcctatattc ccaagaagag tctttccata tcatcgatag
      901 gtatatcggg aagccagggc ttagtaggtt atagtcagtt cgatatgccc tttggggctt
      961 ttcagcttag aacgtcctat gcggaagaaa aaccaagagg tgatatttca cacgaaaaac
     1021 caagagatag tatcctagtg gataaaccgg ataatattgt cctcggagag acagaagagg
     1081 taaaatacag gcatctgccc cctataaccg tcggagtttc cttggccatc aatttcactc
     1141 aacgacttgt acttatcacc gggttggatt actcattata tctttcagag aaaaaagaag
     1201 tcatatctcc cgacaaagtg agaattgaaa ggcaacaact ccattatttg ggattgcctt
     1261 ttagattaaa ctacacgatt tattcaaaaa aatcatttga atggtatgcc ggcggaggtt
     1321 tcaaagtaga caaatgcata tatgccaaat ccggagccga ggttctaaag gaatccaatt
     1381 tcttattatc tgcattactg actacaggaa ttcaatacaa tattacacgc aacttctcca
     1441 tatattgcga accatattgc agttatttgt tttcggatac aggactgcct tcataccgta
     1501 gtgaaaagcc ttttgaaata tcggcgagtc tcggcatcag agtcaattta tgaggtgcgg
     1561 gttagggtat gatcaactat tcttatttcc catcgtaaac tcctgcaaaa agtattacgc
     1621 cggatgattt ttctactatg ccatagacga aaggatggtc ggcaaagaaa tcaatctctt
     1681 tatcaggttg attgggaata gaggtaaatc caatttgcac tgctgtgacg gctgccgcct
     1741 ctgtacccca ttctgctaca tttatacgtg ccttttggat aactgatccg atatagaaat
     1801 ctttgatggc ggaatgggaa agcaatttat cgaattcagc cctattggga tcaaaagcac
     1861 gacgaatacc aagtatgttt aacatcttgg taagctcata tttattctcg gactcaaatt
     1921 tgggaagtcg tagatttaca acggcatcat ttgacattga tgataaggcc ccggaaaagt
     1981 tctccgaagt gaaggatgag aagaggcgct caataccatt ccctcccttt aaatcaggca
     2041 aaatgatata catggcaaac tttccgtgtg catacggaag ttccacgata gcattttttc
     2101 caatctttcc atagcgtaaa ctccttgagg taaccatata atcaactttc ctttctccac
     2161 cgccatccag atagaaaggt tgtgatttct ttgtagattc tttttcaaac attggtttcc
     2221 ctgactcctc actccatttt gctttgaaat agagtgcatt tagaattgcg gcagcaaaat
     2281 tggggataat atcactttcc tggaggaatg gaaatataaa tccgtttgta ctgcgatatg
     2341 cccattcatt gatcctgtcc acaacaacct ttgggttggt tgcatctata tactcgacag
     2401 gggcgtaata gaccttttca gctatatttc ggaaatcctc ctgtactcga aaatccttat
     2461 ttacaataac cgcatcggtc aacttgacct cgacggaatc atcaagcgcc ggcaactgat
     2521 tgagcaaaag attgcaatac tcattaaggg ccttctggtc ttttccatat cctaacgtac
     2581 cgataatctc cgaagccgtt tcaccactgg ccccgtttgc agtaatcgca agggcatatt
     2641 gtaagctgag tggagagaaa ataacactct ctttttcttg agcgtatagg agtttcaaac
     2701 tattaatcgc aaaagcattg cctgctccga cataagcctt ttgttctgca gtcaaggtca
     2761 cttttgttat catacgttcc ggagatggag actcaatctc aataggatct tccacctgcg
     2821 gcacatcatt tttttcacat gctgacaaac ccagggcagc aataaaaaat aataaaataa
     2881 tttttacctg ttccataact gtacaaatgt aggataaata atcgtaaaac tcaacacctc
     2941 atatagttta gtagtatttt gatatagcaa attctatcta tcatatatac gcaataacag
     3001 accaaatact tccataagtt tcaaaaaaat agttgcgtgt tgcgtggtgg aaggttagag
     3061 gttttggatg cgtgttacgg ggagaaagtt agttctgagt tccgagtgtg tagtatgtag
     3121 taagacgatt gaagtctcca ccaaagagaa aaggcaaccg aagttggttg ccttttcctt
     3181 tggtggagat agtcggagtc gaaccgacga cttcctgctt gcaaagcagg cgctctagcc
     3241 aactgagcta tacccccata tggtagtctc tactggaatt gaaccagtga cctctacatt
     3301 atcagtgtag cgctctaacc aactgagcta agagactata taaaaggaag acaaaaagcg
     3361 aaacgccttt gttgtatcaa gtcaaactcc aaaaaggagg tgttccagcc acaccttccg
     3421 gta
//
LOCUS       DAOSIE010000152         3422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_24128_length_3422_cov_3.836353, whole genome shotgun sequence.
ACCESSION   DAOSIE010000152 DAOSIE010000000
VERSION     DAOSIE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24128_length_3422_cov_3.836353"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLI69_05115"
     CDS             <1..978
                     /locus_tag="PLI69_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41901.1"
                     /translation="TFNMHHLIASFPIWKSSAFKVGIVPFSNVGYKFVTSESNDDIIA
                     EAGDIKYSKIGQGSIYQTFFGAGATFWDRLSVGADLQFYFGTIDRYSSATFTTNTSFR
                     SINSGWTFVSRAVSGKFGLQYRQPLAGNNSATIGITYDLPTIFRGENIRYAYGLSASG
                     KDTIFYRNTPLETMSVPAELGVGFTIRNTEKWMVGFDYTRSNWTKAYAEETPGVEVKP
                     ATANAFRVGFEVTPNRYDIRYYLKTLTYRAGAYYEQNYFTLNGSTITRQGITLGVSFP
                     VFRYYNAVTFGIDIGQRGTVENNLIRERYFMFTLSFDLHDIWFVKTLYQ"
     gene            1026..2348
                     /locus_tag="PLI69_05120"
     CDS             1026..2348
                     /locus_tag="PLI69_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41902.1"
                     /translation="MKKIAIILFAMVISLPAFSQGKYGKDSVECTKYLSFYSELYKQR
                     NYDEAAPHWRKAISLCPPTASQNMLLHGQNLLRMDIRKNANNPARRQELIDSLIMLHD
                     LRAEYYPSNRIKSLDNKAIDIINYNYLGDDPLKQYQVLSEIIDAIHTDCSPIVFINQM
                     KGGVALYQAGTLPAESLMTCFEENIGHIEEMIDMDPEKEEYRIVKQDIETIFAESGVA
                     SCDNLIELYTPRFKANPKDVNLLSTMVKMLTRSDCVSTDLFLKSAVALHEINPSSSSA
                     YGLYRLYSSREENTVAARFLESAISLLDPNDMKTAADYNFELATFYFKKLNRSATAVA
                     AAKKAIEYDRSVEGKAYLLIGTIWASQDCQGNEVDARSKYWVAVDYMVKAKNADPEVA
                     ANADELAAQYRKYFPMQVDAFMYDVTDGDRYTVSCGGMTEITTVRTQK"
     gene            2353..2997
                     /gene="lptC"
                     /locus_tag="PLI69_05125"
     CDS             2353..2997
                     /gene="lptC"
                     /locus_tag="PLI69_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOG41903.1"
                     /translation="MTPAVHYRYKPIAIGTCLMAIVLFTLSCKKEPTGDPIDPAVTPL
                     QVVKNMEVVQTVNGGIQMRISAPEMRKYSYIADSAEVSYDLYTGGFSVDAFTPDGELE
                     TRIRSKEAKHVTTDEKEEWLAYGEVVITNYIKQESIESDTIYWDRHAQKFHTNCYVRL
                     SSPQGMMQGYGLESDEMARNAILLKPFDSYTISGDSTTILLDSLNFVGPPVQRF"
     gene            3101..>3422
                     /locus_tag="PLI69_05130"
     CDS             3101..>3422
                     /locus_tag="PLI69_05130"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOG41904.1"
                     /translation="MAVLEKIRVKLGILITVLIAVALLSFIIDPQTLTSTAQRFSSDN
                     KVGVLDGKSISYIDFYQEVDYFTRLTEMMGQSSNSEEVQKAVRDAAWQSLLDKHLFQP
                     MIADA"
BASE COUNT          977 a          822 c          766 g          857 t
ORIGIN      
        1 actttcaaca tgcaccacct catagcctcc ttcccaatct ggaagagttc cgctttcaag
       61 gtgggcatag tgcctttcag caatgtcggg tacaagtttg tcacctccga gagcaatgac
      121 gacataatag ccgaagccgg agatataaaa tactcaaaga ttggacaggg aagcatttac
      181 cagacatttt ttggagcggg agccaccttc tgggatagac tcagcgtcgg agctgactta
      241 cagttttatt tcggtacgat agatcgctac agcagcgcta cgtttaccac caatacctca
      301 ttcagatcca tcaattcggg ttggaccttt gtcagccgtg cggtatcggg caaattcgga
      361 ctgcaataca ggcagccgct ggcgggcaac aattctgcca ccatcggaat aacttacgac
      421 ctgcctacca ttttccgggg tgagaatata cgctacgcct acggattgtc ggcgagtggc
      481 aaggatacca tattttatag aaacactccc ctcgagacca tgtcggtacc cgctgaacta
      541 ggagtgggat ttaccatacg caatacggag aaatggatgg tgggatttga ttacacgcgc
      601 tccaactgga ctaaagccta tgcggaagag acccccggag tggaggtgaa acctgcaaca
      661 gccaatgctt tcagggtggg attcgaagtg acccccaacc gctacgacat cagatattat
      721 ctaaagacct tgacctatag ggcaggtgcc tattatgagc agaactattt taccctgaac
      781 ggctctacca ttaccaggca gggaataacc ctcggagtct cgtttccggt attccgctac
      841 tacaatgcgg tgactttcgg aatagatata ggccagagag gtacggttga aaacaacctt
      901 atcagggaaa gatatttcat gtttaccctc tcgtttgacc tgcacgatat ctggtttgta
      961 aaaactttat accaatagat ttgattattt ttattaaatt tgccaaatat ttaaaaatgg
     1021 aaacaatgaa gaaaatcgca attatcctct ttgcaatggt tatttctctt cctgcttttt
     1081 ctcagggaaa gtatggaaaa gatagtgtgg aatgtaccaa atatctctcg ttttattcag
     1141 agctttacaa acagagaaat tacgacgaag ctgctccgca ctggagaaag gccatatccc
     1201 tttgtcctcc gacagccagc cagaatatgc tcctgcacgg tcagaatctt ctccgtatgg
     1261 acattcgcaa gaatgccaac aatccggctc gcagacagga gcttattgac tctttgataa
     1321 tgctccacga ccttcgtgca gagtactatc ccagtaaccg tataaaaagt ttggacaaca
     1381 aggctatcga tatcatcaac tacaactatc taggcgatga tcctctcaag cagtaccagg
     1441 tgttgtctga gatcatagat gccatacata cggattgtag ccccatcgtc ttcatcaatc
     1501 agatgaaagg tggtgtagct ctctaccagg ccggcaccct cccggcagag tctcttatga
     1561 cctgctttga agagaacatc ggccatatag aagaaatgat cgacatggat cccgaaaaag
     1621 aggagtatcg tatagtgaaa caggatattg agaccatatt tgccgagagc ggtgttgcct
     1681 cttgtgacaa cctcatagag ctttatactc cccgttttaa ggcaaatccc aaagatgtca
     1741 atcttctttc cacaatggtg aaaatgctta ccagatcaga ctgcgtaagc accgacctgt
     1801 tcctcaagag cgcagtcgct ctccacgaga taaatccctc atccagctcc gcctatggtc
     1861 tttacagact ttattcttca cgagaagaaa atacggtggc agcacgcttc ctcgagagcg
     1921 ccatttcgct tctcgatccc aatgatatga agacggcagc ggattacaac ttcgagttgg
     1981 ctactttcta ttttaagaag ctcaatcgct ctgcgacagc agttgcggct gccaagaagg
     2041 ccatagagta tgaccgtagc gtagaaggta aggcatatct ccttatcggc accatctggg
     2101 catcacaaga ttgtcagggc aatgaagtag acgcaagatc caaatattgg gttgcggttg
     2161 actatatggt aaaggccaag aatgccgatc ccgaagttgc agcaaatgcg gacgaacttg
     2221 ccgcacaata tcgcaaatac ttccccatgc aggttgatgc ctttatgtat gacgttacag
     2281 acggggacag atacaccgta tcttgcggag gcatgaccga aattaccact gtaaggacac
     2341 agaaataatc tcttgactcc cgcggtacac tacagataca aacctatagc catcggtaca
     2401 tgcttgatgg ctatagtgct ttttacgctc tcctgcaaga aagagcccac cggcgatccc
     2461 atcgacccgg cggtgactcc attgcaggtg gtgaagaata tggaggtggt gcagactgtc
     2521 aacggaggaa tacagatgag aatcagtgcg cccgaaatgc gcaaatactc atatattgca
     2581 gattcggcag aggtgagtta tgacctctat acgggcggat tttccgtaga cgcattcacc
     2641 cctgacgggg agctggagac ccggatcaga tcaaaagagg cgaaacacgt aactaccgac
     2701 gaaaaggagg agtggctcgc ctacggagag gtagtaatca ccaactatat aaaacaggaa
     2761 agcatcgaga gcgacaccat ctactgggac cgtcatgcgc aaaagttcca tacaaactgc
     2821 tatgtccggc tctcgtcacc acagggcatg atgcagggtt acggcctgga atccgacgag
     2881 atggcacgca atgcaatcct gctaaaaccc ttcgattctt atacgatttc cggtgacagc
     2941 accacaattt tgctcgattc tctcaatttt gttggccccc ccgtgcaacg tttttgacat
     3001 tttatgcatc tatataatac atttttgtct atttttgtgc tccattttac aaaatgggca
     3061 cttaaattag attaaaataa actaaaactt aacaaacaaa atggcggttc ttgaaaaaat
     3121 tcgcgtcaaa ctaggtattc ttataacagt tctaatcgca gtcgcgctgt tatcattcat
     3181 tattgatcct caaacactga cttcgactgc acaaagattt tcatccgaca ataaagtagg
     3241 cgtactggac ggtaagtcca tttcatatat tgacttttac caagaggttg attatttcac
     3301 ccgactgact gaaatgatgg gacagagctc caacagcgaa gaggtacaga aagctgtccg
     3361 cgatgctgcg tggcagtctc ttctcgataa gcacctgttc cagcccatga ttgcagatgc
     3421 cg
//
LOCUS       DAOSIE010000153         3413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_24203_length_3413_cov_4.571471, whole genome shotgun sequence.
ACCESSION   DAOSIE010000153 DAOSIE010000000
VERSION     DAOSIE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24203_length_3413_cov_4.571471"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1184
                     /locus_tag="PLI69_05135"
     CDS             72..1184
                     /locus_tag="PLI69_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG41905.1"
                     /translation="MKELSFSEALSLYLDAPLEELRSMADAARYSHIPQKRVSWQIDR
                     NVNITNACISGCRFCNFHCKPVEKEKIFITTIDQYRRKIAEMRRLGGDQLLLQGGLHP
                     ELDIVYYENLFRELKRIEPSLKLNALGPPEIAFISRISGISIEETLRRLHEAGLDTLP
                     GAGAEILSDRVRKVISPGKPTAKEWCRVMEVAHKMGIGTTATMVYGHIESMEERMVHL
                     FTIRDLQSRRPEGTPGFRAFISWPMQLKGTRLAVIMEERGLQLPRVEYGWSEEEEFLR
                     TIAISRLVLHNISHIQVSWLTVGMETGLKSLHYGADDMGSIMIEENVVSAAGISNTIT
                     AEEMDRAIREAGFEPWLRRQDYSPIYTDILRSQAIS"
     gene            complement(1148..2023)
                     /gene="nfo"
                     /locus_tag="PLI69_05140"
     CDS             complement(1148..2023)
                     /gene="nfo"
                     /locus_tag="PLI69_05140"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOG41906.1"
                     /translation="MKLIGAHVSSSGGVENAPLNAAAIGANAFALFTKNQRQWVSAPL
                     SEQSIRLFKENCIQHGFIPDSILPHDSYLINLGHPTSEGLGKSRAAFLDEMQRCEQLG
                     LNRLNFHPGSHLNEISLSECLRRVAESINMALDKTSGVCAVIENTAGQGSNVGFQFEH
                     LAEIIYQVEDKSRVGVCIDTCHSFVAGYDVRSGENESDLFAGGEDTFGKVIEELDRIV
                     GLQYLKGMHLNDAKKPLGSRVDRHETLGNGFIGKGTFIRIMRDARFNGIPLILETPEP
                     EIWDQEIAWLRSMSV"
     gene            complement(2034..>3413)
                     /locus_tag="PLI69_05145"
     CDS             complement(2034..>3413)
                     /locus_tag="PLI69_05145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOG41907.1"
                     /translation="RMRGSTGILFNHIGDAFNNTYEHLPFSLTEAQKRVIKEIRADVV
                     SGSQMNRLLQGDVGSGKTLVALFSALMAVDNGYQACIMAPTEVLASQHYHGTLRFLEH
                     SGVKTALLTGSTKTKERRAIHAGLEDGSIHIIFGTHALIEENVRFLNLGLAVIDEQHR
                     FGVEQRSRLWTKSNMPPHILVMTATPIPRTLAMTLWGDLDVSVIDELPPGRKPIQTIH
                     WGENSRPRLHEFMKSEIKSGRQVFVVYPLIKESEKMDYKNLEEGYEAIVRAFPPPEYR
                     TAVVHGQQKPENKAFDMELFVSGKAHILVSTSVIEVGVDVPNASVMVIESAERFGLSQ
                     LHQLRGRVGRGAEKSYCILMTGYKLSNESRQRIEMMCQTTDGFELAEADLRMRGPGDF
                     EGTRQSGLALNLRIANLGRDSAVLEDARREASEILDNDPLLSSRENALLPKVLQDLKI
                     EIKDYSNIS"
BASE COUNT          824 a          825 c          835 g          929 t
ORIGIN      
        1 aacttaaggg cttcaagccg cttcccggta ttcctgtcga tcctatacga tagtattcag
       61 gtcgctatgc aatgaaagaa ctctcttttt cagaagcgct gtcgctctat ttagatgcac
      121 cgttggaaga gttgcggagt atggctgatg ccgcaaggta cagccatatc ccgcaaaaac
      181 gggtaagctg gcaaattgac cgcaatgtca atatcacaaa tgcctgcatc agcggttgtc
      241 gtttctgtaa ctttcattgc aaaccggtcg aaaaggagaa aatattcatt actactattg
      301 atcagtacag gaggaaaatc gctgagatgc gtcgtcttgg aggtgaccag cttctgcttc
      361 aaggtggttt gcaccccgaa ttggatatag tttattacga aaatttattt cgcgaactca
      421 aaagaataga gccctcgctt aaactcaatg ctctggggcc tcctgagatt gccttcattt
      481 cccgcattag cgggataagc attgaggaaa cgctacgccg tctgcatgag gcgggactgg
      541 acacactccc tggtgcgggg gctgaaatac tttcggatag agtccgcaag gtcatttctc
      601 cgggtaaacc tactgcgaaa gagtggtgca gggtgatgga agttgcccac aaaatgggaa
      661 taggcactac tgcaacaatg gtatatggcc atatagagag catggaggag aggatggtac
      721 atctcttcac tatccgggat ttgcagagcc gcaggcctga aggcactccc ggattccgcg
      781 ctttcattag ctggcctatg caacttaaag ggacccggtt agctgtaata atggaggaaa
      841 gaggcttgca actgccacgt gtagagtatg gttggagcga agaagaggaa ttcctccgca
      901 caatagcaat ttccaggctt gtcctgcaca atatcagcca tatccaggtc tcatggctca
      961 ccgtgggtat ggaaacaggc ctaaagtcgc tccattacgg agcggatgat atgggctcga
     1021 taatgattga agaaaatgta gtctccgcag cgggcatatc caatacaata actgcagaag
     1081 agatggatag ggccattcgt gaagcggggt ttgagccctg gcttcgccga caagattatt
     1141 ctcctatcta tactgacata ctgcgcagcc aggcaatctc ctgatcccat atttcaggct
     1201 cgggagtttc aagaatcagg gggatgccgt taaatcgggc atccctcatt attctgatga
     1261 aggtgccttt gccgataaac ccattaccaa gagtttcgtg gcgatcaacc ctggaaccaa
     1321 gcggtttttt tgcatcgttg aggtgcattc ccttgagata ttgcagacct acaatcctgt
     1381 ccagctcttc tattactttg ccaaaagtat cctcgccccc tgcaaaaagg tcactttcgt
     1441 tctctccgct ccgaacatca taaccggcaa caaaagagtg gcaggtatca atacagaccc
     1501 ctactctcga tttatcttca acctgataga tgatttcggc aagatgctca aattggaaac
     1561 ctacgtttga gccttgtccg gcggtatttt caatgactgc acagacacct gatgttttgt
     1621 ccagggccat atttatggat tctgcaacac gtctgaggca ttcgcttagg gagatttcgt
     1681 tgagatggct tcctggatgg aaattgagac ggttgagtcc gagttgttca caacgctgca
     1741 tctcgtcaag aaatgccgcc cttgatttgc ccagtccttc agatgtcgga tgtcccagat
     1801 ttatcagata actgtcgtgg gggagtattg aatccggaat aaatccatgt tggatgcagt
     1861 tttctttaaa aagtcggatg ctctgctctg aaagcggagc gctgacccac tgacgctgat
     1921 tttttgtaaa tagggcgaaa gcatttgccc ctattgcagc tgcattgaga ggagcatttt
     1981 ccactcctcc gctactgcta acatgtgcac cgattaattt catttttttc ttttcaactt
     2041 atattggaat aatcttttat ttctattttt aaatcttgca ggactttggg taggagggca
     2101 ttctctctac tcgacagcaa agggtcatta tccaaaatct ccgaggcctc tctgcgtgca
     2161 tcttctagca cggcagagtc cctgccgagg ttggcaatgc ggaggttcaa tgcaagtccg
     2221 ctctgtcttg taccctcaaa atcgcccggt cctctcattc tgaggtcagc ttccgcaagt
     2281 tcaaaaccgt ctgttgtctg acacatcatc tctatccttt ggcgggattc gttgctgagt
     2341 ttgtagccgg tcatcagtat gcagtaagat ttctctgcac cccgtccgac tctgcccctg
     2401 agttggtgga gttgagatag tccgaatctt tcggcacttt caattaccat tactgaagca
     2461 ttcggcacat ccactccaac ttcaatgaca gaggtggaaa ccaagatatg ggcttttcca
     2521 ctcacgaaaa gctccatatc gaaggcttta ttttccggtt tctgctggcc atgaactacg
     2581 gcagtacggt attcgggagg agggaatgct cggacaatag cctcgtagcc ctcttcaagg
     2641 tttttgtaat ccattttctc cgactcctta ataagaggat aaactacaaa tacctggcgt
     2701 cccgatttga tctcactctt catgaattcg tgcaggcggg gacgggagtt ttctccccag
     2761 tggatagttt ggatcggctt tctgcccgga ggcaactcat ctatcaccga tacgtccaga
     2821 tccccccata gagtcattgc gagggtcctc gggatagggg ttgcggtcat taccaatatg
     2881 tggggcggca tattcgactt tgtccagagc ctcgacctct gctctacgcc gaagcggtgc
     2941 tgttcatcaa taaccgccaa tccgaggttg agaaaacgta cattttcttc aatgagcgca
     3001 tgggtgccga atattatgtg gatggagcca tcttcaagtc ctgcgtggat tgcacggcgt
     3061 tctttagtct ttgtggagcc tgtgaggagt gcggttttga ctccgctatg ctccaggaat
     3121 ctcagggtcc catgataatg ttgtgaagca agtacctccg ttggtgccat tatgcaggct
     3181 tggtacccat tgtctaccgc catcagtgca ctgaatagtg ccacgagagt ttttccgctg
     3241 cccacatctc cctgcaggag gcgattcatc tggcttccgg aaaccacatc agcgcggatc
     3301 tccttgataa ccctcttttg ggcttcggtc agagagaagg ggaggtgctc ataagtgtta
     3361 ttaaaagcat ctcctatgtg gttgaacagg atcccggtgg agccgcgcat ccg
//
LOCUS       DAOSIE010000154         3409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_24242_length_3409_cov_3.521169, whole genome shotgun sequence.
ACCESSION   DAOSIE010000154 DAOSIE010000000
VERSION     DAOSIE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24242_length_3409_cov_3.521169"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..635)
                     /locus_tag="PLI69_05150"
                     /pseudo
     CDS             complement(1..635)
                     /locus_tag="PLI69_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
     gene            complement(720..>3409)
                     /locus_tag="PLI69_05155"
     CDS             complement(720..>3409)
                     /locus_tag="PLI69_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOG41908.1"
                     /translation="VIPAGVYQRTFTLPQDWKGKEIYLNLDGAKSGVYLYINGVEVGY
                     SENSKDRAQWKITHCLKSGANHLVIKIMRWSTGTWLECQDFWRISGIERDIYLSAQEP
                     TSLRDFTVTPILSESGEGQLTLRMELFNPDSHQVEAGFELLDKNGVTVASGSSATNNL
                     SVSFNARIENVKPWSAEHPNLYNLIMRVENEYIPFKVGFRRFEIKDGLFFVNGQPIKF
                     KGVNIHEHDQNTGHYISRELLLKDLELMRQHNINAIRTAHYPQPRLFYELCDELGFYV
                     YHEANIESHGMGYNLREGGTLANNRDFYALHQDRIINMYERCKNYACVTILSLGNEAG
                     NGYNMYKAYEWIEEREKKNPGMHRPIVYERALLEWNTDMYVPQYPSADWFAKMGEEGC
                     DRPVVPSEYSHAMGNSNGGLSAQWRQIRLYPQLQGAFIWDWVDQGFLEKDVKGYEYWA
                     YGGDYGVDMPSDGNFCCNGIIAPDRTPHPSMEEVRHAYTNILFEADDPASGKGFTITN
                     LHYFTSLEGYDVYWFIEGDGKIIRKGKLHFSTAPQCVESFDISYNRVKFKEDIDYYIR
                     FSAVTKEAERLIPAGHEVASAQFPISLRENVTLPRMKKGDALVIDENEELIRIGNKRM
                     QMVYDKSLYTITSLKVDGRERFAEGWGIRPNFWRTPTDNDHGNGMNRKNAIWKEASSN
                     FNASAGVSTDGEDAILKVIYSLPAGNNFEMEYRVSRNGVIHVSTRFNAVETKAGEQTS
                     PLFVPRIGVRFRLAASNDSFSYFGRGPVENYIDRKEGSLIGLYKSSAKGEYVKYVRPQ
                     ECGHHCDVRRIDFADMIILADDYMEFNALRNSVEDFDLGKQSHVNDITSNIRNYVEVC
                     LDWRQMGVGGYDSWGSLPDDYAMIPAGENYEWGFRLIFP"
BASE COUNT          819 a          886 c          753 g          951 t
ORIGIN      
        1 tcttgcatgc agcgaaatac cgccatccgg gttggagcgg gtagcgccat atttgagatc
       61 tcctttaatt ggagaaccaa tggcggagag ctggcagcgg atctggtgat ggcggccggt
      121 gagcagttct acctctacaa gccagtaacg ctcggtctgc tccagggttt tgtagcgcag
      181 acgggcctct tttgcacctt cgggaactct ggctcctgct ccgacaggat aggcatagct
      241 acggttttgc ttttcatttt ttaatagaaa atgaaccaat tcgccctcaa ggggctcagg
      301 cctgatagat gtcagagccc aataatattt ctctacgctc ccttcccgaa acatggcatt
      361 gagcctttca agagatttgg atctcttggc aaaaatggtg acaccgctta ccggtctgtc
      421 gagacgatgc ggtactccca tgaagacatt cccggcttgg agtcacgttg agccacaaat
      481 gccttcaaag tctcgctcag cggctcgtct ccggtcttgt caccctgcac aatctctccg
      541 ctacgcttat tgaaaataag catgtggttg tcttcgtata gaatcctcga agcaatatct
      601 tcaaactccg tttctgaaag tctcctgtat accatttttc tttcctgatc aggcttacgc
      661 ttttcaattt cccacaaagt taacaaaact cgttgagatt tttctctccg caaaacgagc
      721 taagggaata tcagcctgaa tccccattcg taattttctc cggcaggaat catggcatag
      781 tcatccggca gtgagcccca actatcatat cctcccactc ccatctgtcg ccaatcgaga
      841 cagacctcta cataattcct aatattggag gtaatatcgt ttacatggct ctgttttccc
      901 agatcgaaat cctccaccga attgcgcagg gcattgaatt ccatataatc gtccgccaaa
      961 atgatcatat ctgcgaaatc tatcctgcgc acgtcgcagt ggtgaccaca ttcctgtggt
     1021 ctgacatatt tgacatactc accctttgcg ctgctcttat aaagtccgat aagagaaccc
     1081 tccttgcggt cgatgtagtt ttccaccggt ccccgaccga aatagctgaa gctgtcgttg
     1141 gaagctgcaa gtctgaaacg cactcctatc ctgggaacaa ataggggaga agtctgctct
     1201 ccggccttgg tttcaacagc gttaaaacgt gtagaaacgt gtattacacc attacgactc
     1261 acacggtact ccatttcgaa attatttcct gcaggaaggg aatagatgac ttttagaata
     1321 gcatcctctc catccgtcga gacgccggcc gatgcattaa agttacttga ggcctccttc
     1381 caaatggcat ttttacggtt cattccattg ccatgatcat tgtcggtagg tgttctccag
     1441 aaattgggcc tgatgcccca gccttcggca aaacgctccc tgccatccac tttcagcgag
     1501 gtgatggtat agagtgattt gtcatagacc atttgcatgc gtttattgcc gatacggatt
     1561 aactcctcat tttcatcgat caccaaggca tcaccttttt tcattctcgg aagagtaaca
     1621 ttctcgcgaa gcgaaatagg aaactgagcc gaagccacct cgtggcccgc aggaatcagt
     1681 cgctccgcct ctttcgtcac agccgaaaag cggatatagt agtcaatatc ctccttgaac
     1741 ttcactctat tataggaaat atcaaatgac tccacacact ggggtgcagt agagaagtgt
     1801 agttttcctt tacggataat cttgccgtct ccctctatga accagtaaac atcatatccc
     1861 tccagagagg taaaatagtg gagattggta atggtaaatc ctttgcctga ggcgggatca
     1921 tcggcttcga acagtatatt ggtatatgcg tgacgaacct cctccatgga gggatgcggg
     1981 gtgcggtcgg gagctataat accgttgcag cagaagttgc cgtcgctggg catatctaca
     2041 ccataatccc ctccgtaggc ccaatactca tatcccttta catccttctc gaggaacccc
     2101 tgatctaccc aatcccagat aaaagcccct tgtagttggg gatagaggcg gatttgtctc
     2161 cattgtgcgc tcaaacctcc gttggaattg cccattgcat gagaatactc gctcggtact
     2221 acggggcggt cgcatccctc ttcgcccatc tttgcaaacc agtcggcaga ggggtattgt
     2281 ggcacataca tatcggtgtt ccactccagc agcgcccttt catagacaat aggacgatgc
     2341 attccgggat ttttcttttc gcgctcctca atccattcgt aggccttgta catattgtac
     2401 ccgtttccgg cctcattgcc caaagagagg attgtaacac atgcatagtt tttacatctc
     2461 tcatacatat ttattatgcg gtcttggtgc agtgcgtaaa agtcgcggtt attggccaga
     2521 gtgccgccct cccgtaaatt gtaccccatc ccgtgagact cgatgttggc ctcgtgatag
     2581 acatagaagc ccaactcatc gcacaattca tagaataatc tcggctgggg atagtgcgct
     2641 gtccggattg cattgatatt gtgctgacgc atcagctcaa ggtccttaag cagaagttcc
     2701 ctgcttatat agtgtccggt attctggtcg tgctcgtgga tattgactcc tttaaacttt
     2761 attggttgtc cattgacaaa gaaaaggccg tccttgatct caaatctgcg gaatcccact
     2821 ttgaatggga tatattcatt ttcaaccctc attatcagat tatagaggtt tggatgctcc
     2881 gccgaccatg gtttcacatt ctctattctc gcattaaaac tcacactgag attgtttgta
     2941 gcgctacttc cggaggcaac tgtgactcca ttcttatcca gcaactcaaa ccctgcctcc
     3001 acctggtggg aatcgggatt gaataactcc atacggagcg taagctgacc ctcgccggac
     3061 tcgctgagga ttggagtgac tgtgaaatcg cgaagcgaag ttggctcctg agccgagaga
     3121 tatatatctc tttcgatacc gctgatcctc cagaaatcct gacactccag ccaggtgccg
     3181 gtactccacc tcataatctt tattacaaga tggttggcgc cggatttgag gcagtgggtg
     3241 attttccatt gcgccctatc cttggaattt tcgctataac caacttccac accatttata
     3301 tagagataga cgccggattt tgctccgtca agattgaggt atatctcctt tcctttccag
     3361 tcttgaggta gtgtgaaggt gcgctgatag actcctgcgg ggattactt
//
LOCUS       DAOSIE010000155         3374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_24587_length_3374_cov_4.457065, whole genome shotgun sequence.
ACCESSION   DAOSIE010000155 DAOSIE010000000
VERSION     DAOSIE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24587_length_3374_cov_4.457065"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLI69_05160"
     CDS             complement(<1..279)
                     /locus_tag="PLI69_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG41909.1"
                     /translation="MKRIIYLLLATLFVFASCNNYSYESVPGDPLGARIYTLDNGLKV
                     YMVVNKDEPRVNAHVAIRVGSKNDPVETTGLAHYFEHLMFKGTEKFGTQ"
     gene            375..1484
                     /locus_tag="PLI69_05165"
     CDS             375..1484
                     /locus_tag="PLI69_05165"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOG41910.1"
                     /translation="MKKLLIIISLILSQFCVYQMVAQEPQVMLDESKVISFDKKIHDF
                     GEVMISDGPVKCTFTFTNISDSPIVVHNIISSCGCTTPQWTRSPVKPGGKGKVDVVFN
                     NDQGPFPFDKTLTLYVSGVGRPVVLRIRGIAYEKSKNVDELFTGRIGQMGINKSAVSL
                     RNIEQGVLKQDEIDIANLSSSPLKVETREVSRGLLLTVYPNPIPPKSMAKLTYTVDPT
                     LMPKRSWGKQTFTGRFVLNGKKQPEEFTVSAVIVDNFRDMNAKQIKNAPLPIVEKPYY
                     SFGTAVKGTVLDISYKIKNKGKSELLIHTVESEKSGLSQIGPTPLRIKAGESATLKLK
                     FDTREHSGEMINILTLITNSPSDPMVNIFITGTVK"
     gene            1515..2636
                     /gene="meaB"
                     /locus_tag="PLI69_05170"
     CDS             1515..2636
                     /gene="meaB"
                     /locus_tag="PLI69_05170"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOG41911.1"
                     /translation="MKNKPQGDFFADYNNEDTALVINKGVEQPPIINPYVKSFYKKKP
                     SLLSVDKYMEGITAGDTTILSQAITLIESSLVEHREIARKIIERCQIISARQHSMRIG
                     ITGVPGAGKSTFIEAFGSFLTSKGYKLAVLAIDPSSERSKGSILGDKTRMETLCNDPN
                     AFIRPSPSAGSLGGVARKTRETILLCEAAGFDVIFIETVGVGQSETAVHSMSDFFLLL
                     MLSGAGDELQGIKRGIMEMADLIAITKADGTNISKSNMARALYSNALHLFPPTESGWV
                     PTAVTSSAVTKEGLPAILQKIEEYFALVKANGYYFKKRREQARFWMYESINESLKTMF
                     YENPLIEKLLPEYESSVLEGEIESFTAATELIEKYKSTK"
     gene            2648..>3374
                     /locus_tag="PLI69_05175"
     CDS             2648..>3374
                     /locus_tag="PLI69_05175"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOG41912.1"
                     /translation="MNWSLFANVLKNGVLITGLVTVIMLMIEFINVSSHGKWFERLKG
                     APNKQVFLGTLLGLIPGCVGGFAAVSLYTHRLIGFGALVAMMIASSGDEAFVMLALIP
                     KDALILFGVLFVIAIVSGLLINLFARLRRGEKRFKTACKDEFEVHTEDHHHTFETPHF
                     SIANIKYFSWRKIVLMVALTAFIVALGFGVLGHDHHEATQIVTETVSPEHDHFHDHAE
                     DQLHDHAEDHFHHHAHGISFLDEY"
BASE COUNT          980 a          724 c          799 g          871 t
ORIGIN      
        1 ctgagtaccg aacttctccg tgcccttgaa catcaggtgc tcaaagtagt gggccaaacc
       61 ggtggtttct acggggtcat tcttgctgcc tacgcgtatg gctacgtggg cattgacgcg
      121 gggttcatct ttattgacaa ccatatagac tttgagtccg ttgtcaagtg tgtagattcg
      181 tgcccccagt gggtcaccgg gaactgactc gtaagaatag ttgttgcaag atgcaaatac
      241 aaatagggtt gcgagaagca gatagataat tctcttcatt gtagtgttgg attatgtttg
      301 tttggttcga tttatgcaaa taataaacaa aaatagcaaa ctatatttat tatctttgcc
      361 aaaaattaaa taaaatgaag aaactgctga tcataataag tttgattctg agccaattct
      421 gtgtctatca aatggttgcc caggagccgc aggtaatgct cgatgagagc aaggttatta
      481 gtttcgataa aaaaatccac gatttcggag aggttatgat ttcggatggg ccggtaaaat
      541 gcacatttac attcacaaat atcagtgatt caccgatcgt ggtgcataat atcatttcat
      601 cctgcggatg taccacaccc caatggaccc gttctccggt aaaaccgggc ggaaaaggca
      661 aagtggatgt ggtgttcaac aatgatcagg gaccttttcc ctttgataag acccttacac
      721 tctatgtttc gggtgtcggt cgtccggttg tcctaagaat caggggtata gcctatgaga
      781 aaagtaaaaa tgtagatgag ctcttcaccg gtagaatcgg acagatgggc ataaataaaa
      841 gtgcagtctc ccttcgaaac atagagcagg gtgtactcaa gcaggacgaa atagatattg
      901 ccaatctctc ctcatctcct ctgaaggtgg aaaccagaga ggttagccgc ggacttctgc
      961 tcacggttta tcccaatcca attcccccca aaagtatggc caaactcact tataccgtgg
     1021 atccaaccct catgccgaag cgtagttggg gcaaacagac cttcacagga cgcttcgtcc
     1081 ttaatgggaa aaaacagccg gaagagttta ctgtgagtgc cgtgatagtg gacaattttc
     1141 gcgacatgaa cgcaaagcag ataaaaaatg ctccattacc aattgtagag aagccttatt
     1201 acagttttgg taccgcagtc aaaggtactg tactagacat ttcctataaa atcaaaaata
     1261 aaggcaaaag tgaactgctg atacatacgg tggagagcga aaagagcggt ttgagtcaga
     1321 ttggtccaac gccgctccgc attaaagcag gagaaagcgc taccctgaaa ctcaagtttg
     1381 atacaagaga acattccgga gaaatgatta atatccttac tttgataaca aattctccat
     1441 ccgatccgat ggtaaatatt ttcatcacag gtaccgtcaa atagaactaa acagctcttt
     1501 taaccggaaa caatatgaag aacaaacctc aaggagattt ttttgcagat tacaataatg
     1561 aagataccgc acttgtaata aacaagggag tggagcagcc gcccatcatc aatccctatg
     1621 taaagtcatt ttacaaaaag aaaccctcct tgctttccgt ggataagtat atggaaggaa
     1681 taacagccgg agatactacg atcctcagtc aggcaattac actaatagag agctcattgg
     1741 tggaacaccg cgagattgcg cgaaaaataa ttgagcgttg tcagataata tctgcacggc
     1801 agcactccat gagaataggt attaccggag tgccgggagc gggaaaaagt acctttatag
     1861 aggcttttgg ctcttttcta acctctaagg gatataagct cgcagtgctg gcgatagatc
     1921 cgagttccga acgcagcaag ggaagtattc ttggggacaa gaccagaatg gaaactctct
     1981 gtaatgatcc caacgccttt attcggccca gccccagtgc gggttcgctc ggaggtgtgg
     2041 cacgaaagac ccgagagaca attttacttt gtgaggctgc cggcttcgat gtcatattca
     2101 tagagacggt gggagtaggg cagagcgaaa ctgccgttca ctccatgagt gacttttttt
     2161 tgctgctgat gctctcggga gccggagatg aacttcaagg aatcaagcgc ggaatcatgg
     2221 aaatggccga cctcatagcc attaccaagg ctgatggcac caacatatcc aaatccaata
     2281 tggcgcgtgc tctctattcc aatgcactgc acctgttccc tccaacggaa tcgggctggg
     2341 taccaacggc tgtaacctct tcagcagtta caaaagaggg actcccggct attttgcaga
     2401 agatagagga gtattttgct ttggtcaagg caaacggcta ttactttaag aagcgcaggg
     2461 aacaggccag attttggatg tatgaaagca ttaatgaatc actcaagact atgttttatg
     2521 aaaacccctt aatagagaaa cttctgcccg aatacgaaag ttcggtgctg gagggggaga
     2581 ttgagtcatt tacggctgca acggaactaa ttgaaaaata taagagtact aaatagagag
     2641 agaagttatg aattggagtc tctttgcaaa tgtgctgaaa aacggagttc tcattaccgg
     2701 actggttacc gtaataatgt tgatgataga gttcatcaat gtttcgtctc atggaaaatg
     2761 gtttgagcga ctaaagggag cacccaacaa acaggtcttt ttaggaaccc ttttgggact
     2821 tataccagga tgtgtaggtg gattcgcggc ggtatcgctc tatacgcacc gcctgatagg
     2881 attcggcgct ttggttgcaa tgatgattgc atcgtcggga gatgaggcct tcgtgatgct
     2941 ggcccttatc cctaaagatg ccctgatact ttttggagtt cttttcgtca ttgctatcgt
     3001 ctcgggactt ctgatcaacc tgtttgcccg cctgaggaga ggcgaaaaaa ggtttaagac
     3061 tgcctgtaaa gatgagtttg aggtgcatac ggaagatcac caccacacgt ttgagacccc
     3121 tcatttcagt atagccaata tcaagtattt ctcctggcgg aaaatagtgc ttatggtggc
     3181 ccttactgcc ttcattgtgg ctttgggatt tggtgttctc ggccacgacc accatgaggc
     3241 gactcaaatt gtcactgaaa cggtctctcc ggagcacgat catttccacg accatgcaga
     3301 agatcagctc cacgaccatg cagaagacca tttccaccac catgcacacg gtatctcgtt
     3361 tctcgatgaa tact
//
LOCUS       DAOSIE010000156         3366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_24661_length_3366_cov_3.569012, whole genome shotgun sequence.
ACCESSION   DAOSIE010000156 DAOSIE010000000
VERSION     DAOSIE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24661_length_3366_cov_3.569012"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLI69_05180"
     CDS             complement(<1..543)
                     /locus_tag="PLI69_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG41913.1"
                     /translation="MGRLRIRLKGDKIIWILVLCLAIISLLAVFSSSTYLANKEGVSK
                     LHILQEQLMSVSIGFGTLLICYLIPMRFYRFLAFPLFGITVLMLILLFITPDVRNEAA
                     RGIRLFGRTIQVFEFAKVGLILYIAKALEYWENSLTTYKEFLIKILLPIVAVCLMVMV
                     NSVSSALLFGVISILLLFFMN"
     gene            complement(550..1884)
                     /gene="murD"
                     /locus_tag="PLI69_05185"
     CDS             complement(550..1884)
                     /gene="murD"
                     /locus_tag="PLI69_05185"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOG41914.1"
                     /translation="MKRIVVLGGGESGSGAAVLAKVKGFEVFLSDNGKIEEKYAARLS
                     EYGIPYEEGGHTIEKIYNADEVIKSPGIPDTALLIIGLNARGISVISEIEFAGRYDNS
                     RKICVTGSNGKTTTTSLIYYLLKNAGLNVGLGGNIGQSYAFQVATQEYDIYVLEISSF
                     QLDGMYEFRADIAVLLNITPDHLDRYGYQMQNYINSKFRITRNMAKEDCFIFCSDDEI
                     TIEHLNRIVIQAQKLPFSQNNVVEQGAYVEGDRMCVRYGDGSYEMFLHELALQGKHNI
                     YNSMAAAITGKIMNITNSAIKDALTTFKGVEHRMEKVLSVGGVMYINDSKATNVNSTW
                     YALESIKTPIVLILGGTDKGNDYSPIADLVKEKVRAIVCLGVDNRKIHDFFGDKVAEI
                     HDTRSAEEAVKMAASIAKKGDTVLLSPSCASFDLFHNYEDRGRQFKAAVKNL"
     gene            complement(1891..3189)
                     /gene="mraY"
                     /locus_tag="PLI69_05190"
     CDS             complement(1891..3189)
                     /gene="mraY"
                     /locus_tag="PLI69_05190"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOG41915.1"
                     /translation="MLYHLFEYFEKANIDFAGSGLMKYISFRSFGASIVAILISLIIG
                     HKLIAWLQKKQIGETIRDLGLEGQLQKKGTPTMGGIIIIISILVPVLLFCDLTNINIL
                     ILILTTVWFGTIGFIDDYIKVFKKNKEGLNGWYKIASQVLMGLIVGLIMCFSNEAGFR
                     EQKRVNPVSEITGEVIAVDNAVSSEAVTVDATLLENSTKTTLPFVKNNEFDYGWLSPF
                     GGWFGEYFKWFIYICFIILVITACSNGTNVTDGLDGLATGITAIVGATLGIFAYLSGN
                     VIYSEYLNIMYIPSSAEITVFFSSMVGALLGFMWYNAYPAQIFMGDTGSLTLGGIIGV
                     CAILVRKELLLPLLCGIFFLESLSVVVQRFYFKYTKKKSGTGVRVFRMAPLHHHFQKD
                     NPDAIIQWPGKVYPEQKVVARFIIVSIILAVLTVVTLKIR"
     gene            complement(3209..>3366)
                     /locus_tag="PLI69_05195"
     CDS             complement(3209..>3366)
                     /locus_tag="PLI69_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOG41916.1"
                     /translation="RKEAIRTAIMMANKGAVILVAGKGHENYQIVGGEKHHFDDKEII
                     SETFEQL"
BASE COUNT          925 a          790 c          680 g          971 t
ORIGIN      
        1 attcatgaag aatagcaaca atatactgat tacaccaaac aacagcgctg acgaaacact
       61 gttgaccatg accataaggc agactgctac tataggaagt aagatcttta taaggaactc
      121 cttatatgtg gttaaagagt tttcccagta ttctagtgct tttgctatat atagtatcaa
      181 acctaccttc gcaaattcaa aaacttggat tgttctgccg aaaagccgta tgcctctggc
      241 tgcctcgttg cgtacgtcag gtgttatgaa aagcagtata agcatcaaaa ccgttattcc
      301 aaatagagga aaggctaaaa aacggtaaaa tctcatcggt atcagatagc agataagaag
      361 agtgccaaaa ccgatggata ccgacatgag ttgctcctgt agtatatgaa gtttactaac
      421 gccctcctta ttggccagat aggtgctgga agagaatacc gcaagcaatg aaataattgc
      481 cagacaaagc acaagtatcc agatgatctt gtcccccttg agtcttattc tcaatctgcc
      541 catctcctcc taaagatttt ttacggcagc cttaaactgt ctgcctctgt cctcataatt
      601 atgaaatagg tcgaaactcg cacagctggg cgatagtaga acggtatcac ctttcttggc
      661 aatggaggca gccatcttta cagcctcctc cgcagagcgg gtatcgtgaa tttccgccac
      721 tttatctcca aaaaaatcgt gtattttgcg attgtcaaca cccaggcaga ctatggctct
      781 aaccttctct ttcaccagat ccgcaatagg ggagtagtcg ttgcccttat cggttccccc
      841 aagtatcaag acgatggggg ttttgatgct ttccagcgca taccaggtgg aattgacatt
      901 agttgccttg gaatcgttga tgtacataac gccacctact gagagtactt tttccatcct
      961 gtgttctacc cctttaaatg ttgtcagggc atcctttatg gcactgttgg tgatgttcat
     1021 tattttgccc gttatggcag ctgccataga gttgtatatg ttgtgctttc cttgcagtgc
     1081 aagttcatga agaaacattt cgtaactgcc gtcaccatat ctgacacaca ttctgtctcc
     1141 ctccacataa gccccctgct ccacgacgtt attctgactg aaaggtagtt tctgagcctg
     1201 aatgactata cggttgagat gttctatggt tatttcatcg tcagagcaga agatgaagca
     1261 gtcctccttc gccatatttc gggtaatcct gaatttggaa ttgatatagt tttgcatttg
     1321 gtaaccatat ctgtcgagat ggtccggtgt gatgtttagc aatactgcta tatctgcccg
     1381 gaattcatac attccgtcaa gttggaaact gctaatctcc aatacataaa tatcatactc
     1441 ctgagttgcc acctggaagg cgtaactctg accgatattg ccacctaaac ctacattcag
     1501 accggcattt ttaagcaaat agtatataag agacgtagta gtagtcttac cgttgctgcc
     1561 ggtgacgcag atctttcttg agttgtcata tctgccggcg aactcgattt ctgaaattac
     1621 ggatattccg cgggcattga gtcctatgat tagtagtgca gtatcgggga taccgggact
     1681 ttttatcact tcatcggcat tatatatctt ctcaattgta tgtccgccct cctcataggg
     1741 gatcccgtat tccgaaagac gtgcagcgta tttttcctcg attttcccgt tgtctgacag
     1801 aaaaacctca aaacccttga ctttcgccag aacagcagca cccgagccgc tttcaccacc
     1861 acccaatacg acgattcgtt tcataactta ctatctgatc tttaatgtta ctactgtcaa
     1921 taccgcaagt attatagaga ctataatgaa tctggccacc actttctgtt ccggatagac
     1981 ctttccgggc cactgtatta ttgcatcagg attatctttc tggaaatggt gatggagagg
     2041 tgccattctg aacaccctga cacctgtacc gctctttttc tttgtatatt tgaaataaaa
     2101 tctttgcacc acgacagaga ggctctccag aaagaatatt ccgcaaagca ggggaaggag
     2161 caactctttc cttacaagta tggcgcaaac tccgattata cctccgagag tcagactacc
     2221 cgtgtcaccc ataaatattt gagcagggta tgcgttatac cacataaaac ccagcagggc
     2281 acccaccata gatgagaaaa ataccgttat ttccgcactg ctcggaatat acatgatgtt
     2341 gagatactcg gagtaaataa cgttacccga caggtaggca aatattccga gagtggctcc
     2401 tacgatggct gttattccgg ttgccagtcc atcaagacca tccgtcacgt tggtgccatt
     2461 ggaacaagcc gttataacca aaataataaa acatatatat atgaaccatt tgaaatactc
     2521 tccaaaccat cctccgaaag gagagagcca tccgtagtca aactcattat tcttgacaaa
     2581 aggaagtgtg gttttagtgc tgttttccaa aagagttgca tctacagtca ctgcttcgga
     2641 agaaactgca ttatcaactg caatcacttc accggtgatc tccgacacgg gattgaccct
     2701 cttctgctcc ctgaatcccg cttcattact gaaacacatg atcaagccca caatcagacc
     2761 cataagcact tgggaggcta ttttgtacca accgttgaga ccctccttgt ttttcttgaa
     2821 tactttgatg tagtcatcaa taaatcctat cgtgccaaac cacacagtag taaggatcaa
     2881 tatcaggatg ttgatattag taaggtcgca gaaaagcagt accgggacca agattgagat
     2941 gataataatg atgccgccca tggtgggagt gccctttttc tgtaactgac cttccagccc
     3001 cagatcacga atggtttctc cgatttgttt tttctgtagc caggcaatca gtttgtgccc
     3061 tatgataagc gatatcaata tagcgacgat actggccccg aaagacctga atgagatgta
     3121 cttcatcaat ccggagccgg cgaaatctat gttcgctttc tcaaaatatt caaaaaggtg
     3181 gtacagcata ggtagaggtt ttgtccggtt atagttgttc aaatgtttcg gagataatct
     3241 ccttatcgtc aaaatggtgt ttttccccgc cgacaatctg ataattttcg tgtcctttac
     3301 ctgccacaag tattacagcg cctttgttag ccatcattat ggctgtgcgg atggcctctt
     3361 tgcggt
//
LOCUS       DAOSIE010000157         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_24716_length_3360_cov_3.557640, whole genome shotgun sequence.
ACCESSION   DAOSIE010000157 DAOSIE010000000
VERSION     DAOSIE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24716_length_3360_cov_3.557640"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..3251)
                     /locus_tag="PLI69_05200"
     CDS             complement(27..3251)
                     /locus_tag="PLI69_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG41917.1"
                     /translation="MKLLKLNIRNIASISEAEIDFTKDIFANESIFLIYGETGSGKTT
                     ILDAISLALYGRTPRLDNSRTRKTVPTYTLGEGVVNIMDTKQLLKADAQEGEVSLYFT
                     AANGKNYTAQWKVSRLRGSSEIKDPEWIVIDGGRTIEIKPNARIETERRDLDDLVGLS
                     YDQFCKTAMLAQGEFTQFLKSNDEEKCEILEKITGNTSYSIVGTEINLLKNKADNDVK
                     LDKELLKKVEICGLRYDPEESSFSDYATLIDNKISKIEDEARGFDEKVAAATELIAAK
                     EARQSMENADKEYKAEVVTEDACVKTAAALIILQEKRQNEYETQEAFYKEMQRQLESI
                     ATDVPMYEKWDIIESNLQKVPLVEQDISNNEKRTEVVKSQLNTLEAEKKARSEAHETI
                     KRKIAALEEQYSKKEEELALLEPDKKKANKAQLEDFRIKFGEVEKVIGVLAEKEKALK
                     SLLEELAVKKSELEKRKKDIAGLLEKYNLTVTEEKEAGELYEKSAGALEQFVRLNENG
                     AALRAIIKADDDCPVCGRIVTQDILDAFNDDDIRQSIKHLEESRDKGKAAWDDSVKRL
                     NDARTSYDTEFRIFTADNNALQKMENDVLSQTEAISRFKEINRRTLSDCHLDWDVEDI
                     AGQISKSKAGTLSELEKASKVLEIIESLEKEISGMRKGIEEVRKNEENPAAQSSQEAV
                     QKIHDAKTHIAALESALAGLGKERENYMETAGKHITIGDWKDKWNSNRVGFLTQLSER
                     TTDYKKLKKDSQDLQLKVASLRDELDKIKLKRGEYFNTYPDIKKRTEEFDIDVFSPED
                     LEKVASESILTRWAAVMTRTGAAKEQMETIQKKKEEAQGRFNLFKNKYPLAETPDFYL
                     KGDPQLTLDTPREGLERLKNEWLVTLGKLNQTAATERRELSKAQTLEGEIGVKIELAG
                     RWKRLDALFGGKDGKNFRDAAMRMLFKEILVRANESLRHIAPHYQLHNEPGSMVILVR
                     DLEQGGYTRATSTLSGGESFVVSLALALGLSTFGKEGLAVDTIFIDEGFGTLSEDILE
                     RVIITLEDLKRYGCRVGLISHVPALRNRISAKLEVRDGRVRKVC"
BASE COUNT          743 a          877 c          652 g         1088 t
ORIGIN      
        1 ggaacttaaa actcagaact tttaggtcaa cagaccttcc tcactctacc gtcccttacc
       61 tccagttttg cgctgatcct attgcgcagc gcgggaacgt ggctgattag gcccacccta
      121 cagccatatc tcttgagatc ttcgagtgta ataatcaccc tttcgagtat atcctcgcta
      181 agggtaccaa atccctcatc aatgaaaatg gtatcgactg caagcccctc tttgccgaag
      241 gtcgaaaggc cgagtgccag cgccagcgag accacaaagg attctcctcc ggaaagcgta
      301 ctggtggctc tggtgtagcc accttgctcg agatctctca cgagtataac catggagccg
      361 ggttcattgt ggagctggta gtggggtgca atatgccgga ggctctcatt ggctctgaca
      421 agtatctcct tgaagagcat ccgcatggca gcatcccgga agttcttgcc atctttaccg
      481 ccgaataggg catcaagcct cttccaccgc cccgcaagtt cgattttaac ccctatttcg
      541 ccttcaagtg tctgcgcttt ggatagttct cttcgctctg tagcagcagt ttgatttaat
      601 ttgccaagtg tgacgagcca ttcgttcttc agccgctcaa gcccctctct gggagtatcc
      661 agagtgagct gagggtctcc ctttagatag aaatcgggag tctctgcaag gggatattta
      721 tttttgaata aattgaaacg cccctgtgcc tcttcttttt tcttttggat tgtctccatc
      781 tgttccttag cggccccggt acgggtcata acggccgccc atctggtcaa aatcgactct
      841 gatgcgactt tctccaaatc ttcgggagaa aaaacatcaa tatcaaactc ctccgttctc
      901 tttttaatat ccgggtatgt attgaaatat tcccctcttt tgagtttgat tttatccagt
      961 tcatcgcgta aactagctac tttgagttgt aaatcctgag aatccttttt cagtttcttg
     1021 taatcggtgg ttctctctga taattgtgtg aggaatccta cacgattgct attccattta
     1081 tctttccaat ccccgattgt gatatgtttc ccggcagttt ccatatagtt ctccctctcc
     1141 tttcccaacc cggcaagggc actctccaga gcggctatat gagttttagc atcatggatt
     1201 ttttgaactg cctcttgaga tgattgtgca gcgggattct cctcattttt ccgaacctcc
     1261 tcaatacctt tacgcattcc ggaaatctcc ttttccaaag attctatgat ttcaagaact
     1321 tttgaggcct tttccagctc agaaagtgtc ccggctttgc ttttactgat ctgccccgca
     1381 atatcttcaa catcccaatc aagatgacaa tcggagagag ttcttctgtt tatctctttg
     1441 aaacgggaaa tggcttcagt ttgacttagc acgtcatttt ccattttttg cagagcattg
     1501 ttgtctgcgg tgaagattct gaattcggta tcgtatgacg tccttgcatc gttcaatctc
     1561 tttacactat catcccatgc agcttttccc ttatcacgcg actcctcaag atgctttata
     1621 gattgtcgga tatcatcatc attaaatgca tcgagtatat cctgcgtcac gatcctgcca
     1681 caaacggggc aatcatcatc tgctttgatt atagccctaa gtgcggcccc gttttcatta
     1741 agcctgacaa attgctccag cgcgccggca ctcttctcat aaagctcccc ggcctccttt
     1801 tcttcggtta cagtcaagtt gtatttctcg aggagaccgg caatatcttt tttcctcttt
     1861 tccagctccg atttcttgac ggcaagctcc tccagtagac ttttcagggc tttctccttt
     1921 tcggccagaa cgccgattac tttctcaacc tctccaaact taatacgaaa atcttccagt
     1981 tgtgctttat tggccttctt cttatccggt tccagaagtg caagctcttc ctcttttttg
     2041 gaatattgtt cctcaagagc ggctattttc cttttaatcg tctcgtgtgc ctctgatctc
     2101 gcttttttct cggcttccaa agtgttcagt tgagatttta ccacttcagt tcgcttctcg
     2161 ttattagaga tatcctgttc tacaagcgga accttctgta aattgctttc aataatgtcc
     2221 catttctcat acatgggaac atccgttgca atcgactcca attgacgctg catctctttg
     2281 tagaaggctt cctgggtttc atactcattc tgacgctttt cctgaagtat tatcagcgca
     2341 gcagcggttt ttacacaggc atcctccgtg acaacctctg ctttgtattc cttatcggca
     2401 ttctccatac tctgtcgagc ctcttttgcg gcaatcagtt cagtagcagc agctactttc
     2461 tcatcaaagc cccgggcttc atcttcaatt ttggagatct tgttatcaat aagagtggca
     2521 taatccgaaa acgaactctc ttccggatca tatctcagac cacaaatctc tacttttttc
     2581 aataactcct tgtcaagctt gacatcatta tcggccttgt tcttgagcag gtttatctcg
     2641 gttcccacta tgctgtaaga ggtattgcct gtaatcttct ccagaatttc gcatttttcc
     2701 tcatcatttg acttgagaaa ctgtgtaaac tccccctgtg ccagcattgc agtcttgcag
     2761 aactggtcat acgaaaggcc taccagatca tccagatcgc gtctctccgt ctcaatgcgg
     2821 gcatttggct tgatttcaat tgtgcgaccg ccatctatca ctatccattc cggatctttg
     2881 atttcgctgc tgccccttag acggctcact ttccattgtg ccgtataatt tttaccattt
     2941 gccgcggtaa aataaagcga gacctcccct tcctgcgcat ctgctttgag aagttgtttc
     3001 gtgtccatta tattgacaac gccctcccca agggtatagg tcgggacggt tttacgggtc
     3061 ctggagttat ccagacgcgg agtgcgcccg taaagtgcca gtgagatggc atccagtatg
     3121 gtggtcttgc cggaacctgt ttcaccatat ataagaaata tggattcgtt ggcgaaaata
     3181 tccttcgtaa agtcaatttc cgcttctgag atagaggcaa tgttccgtat atttagtttg
     3241 agaagtttca tactatactt tcagccatta gtctcgtttt tcaattacac atccattttt
     3301 ttcacaaggg cactcccttc aataacaact acgtcgtctt gattataaca cgtagtatcg
//
LOCUS       DAOSIE010000158         3347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_24823_length_3347_cov_3.660693, whole genome shotgun sequence.
ACCESSION   DAOSIE010000158 DAOSIE010000000
VERSION     DAOSIE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24823_length_3347_cov_3.660693"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PLI69_05205"
     CDS             <1..763
                     /locus_tag="PLI69_05205"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG41918.1"
                     /translation="FAFTRRIGRPIYPQLNPYMSMVDASTYEQGNMNLKPEKSSIIDL
                     SYSLKGRYVQLFADAYLNYTTGYISQITKISDGLLITTYINGTSDLKSGTDFSLKLLP
                     CGWLSATLSTNTFYVDTRGNFAGAEINNRGWSNNSNLLLDFLIGKSTDLQIQYFLTTP
                     QYFPQLTTSLTHHMNAGFKHTFMKGAMSLSLLLTDVFNTYKWQVQSSNKIFDLTNIST
                     MKSRMFWVGISYNFNSFKQKNGEAKSESDRTLIKL"
     gene            complement(964..1035)
                     /locus_tag="PLI69_05210"
     tRNA            complement(964..1035)
                     /locus_tag="PLI69_05210"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1000..1002),aa:Cys,seq:gca)
     gene            complement(1109..>3347)
                     /locus_tag="PLI69_05215"
     CDS             complement(1109..>3347)
                     /locus_tag="PLI69_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG41919.1"
                     /translation="RIFMKKTLLSAAFLLLCALLSAQQSSLNSAPDLHQSHPHYTIDS
                     TIVTASRAQSGTPVTSTEIKREQLSRAGTMTSLPMLLSLQPSVVATNEGGTGLGYSQM
                     RVRGSDATRINVNINGITLNDAESQKVFWVNIPAIAGFLESVQLQRGAGTSVNGAGAF
                     GATLNMSTLGAAENPYAKVALSGGSWLTGSTTVGAGTGRGKSGLSLDMNYSYNTTEGY
                     IRNAFARLHSAYAALGWLRSSDSFRLNWIMGKQRSGITWLGIPADMLESDRRYNSAGE
                     YSDGSGNVLYYDNETDNYLQNHLQAIWVHQFSPHLSMSATFNYTRGAGYYENYKAGKK
                     FSDYGLSEQLVGGVRYKRSDVVVRQQMENNNFAATTNLKYSGEGFFLLGNLSWSLYDG
                     DHYGNVIWAKYNENIPEDWRWYLNRGLKKDLSGFLRGEGDVFGGKLHLYGDIQYRNVN
                     YALSGIDKDFVALDKDFIYNFFNPKGGVSWNFDGRSAAYASLAVAHKEPSRSDIKEAI
                     KAGEGERILPERMMDWEFGYNYTSPLFAFSANLYLMEYRNQLLETGKITEVGYRVKEN
                     VERSYRRGIELSAGWQALRSLRFDANIALSRNRITECNLWVDRYDNPDEWNPLPQAMQ
                     RVENGEILMSPPIVGMAMATWNPLDKLSFSLRGKYVGKQYYDNTSSDERSLPAYTVFS
                     ADVHYKWKNIGISIYADNLLNHKYVADAWVYRATFADGSADYIEAGFFPQARFNLLLK
                     LEYGF"
BASE COUNT          933 a          841 c          733 g          840 t
ORIGIN      
        1 ctttgctttt acacgtagaa tcggaaggcc tatctaccct cagctaaatc cctatatgag
       61 catggtggat gcctcaactt acgaacaagg caacatgaat ctaaaacctg agaaatcctc
      121 aataattgat ctaagttata gcctgaaagg cagatatgtg caattgtttg cggatgccta
      181 tttgaactat actacgggat acatttcaca gataacgaaa atatcggatg gtctgctcat
      241 cacgacctac ataaacggta cttcggactt aaagagcgga acggattttt cactcaaatt
      301 gcttccttgc ggatggttgt ctgctacact gagcacaaac accttctacg tggacacgag
      361 gggaaacttt gcaggagctg aaatcaataa cagaggttgg tcgaataaca gtaatttgct
      421 ccttgacttc ctgattggaa aatccactga cctccagata caatattttc tcacaacacc
      481 gcaatatttt cctcaactaa cgacatccct aacacatcat atgaatgccg gatttaagca
      541 cacattcatg aagggggcga tgagcctatc cctactctta actgacgttt tcaacacata
      601 taaatggcag gtacaatcga gcaacaagat ctttgacttg acaaacataa gtaccatgaa
      661 gagccgaatg ttttgggtag gcatttcgta caacttcaac tccttcaaac agaagaatgg
      721 tgaggcgaaa tcagagagtg acagaaccct aatcaaatta tgattacagt gcctctctaa
      781 tatatgaacc tgctctagat tttaagcaat cggagcgaag cgacaccgca gcgcttctgc
      841 gctgctttgg ttccccaagg gaaccattaa aaggaggggt agataaggag cgaaacgaag
      901 cggagcgact gcacctcaga aaagaaaact ccccgaagtt acgcttcgag gagttctctt
      961 ttgaggtgcc tggcggattc gaaccgccgt aaacggtttt gcagaccgct gactaagcca
     1021 ctcatccaag gcaccggatt cggggctaca aatataacca tttttttcaa gaagcaaaca
     1081 aaataggccc tctaagcaaa ccattgtatc aaaaaccata ttcgagtttt agcagaagat
     1141 tgaatctcgc ttgaggaaag aatccggcct ctatatagtc cgcactacca tcagcaaaag
     1201 tagctctgta gacccaggca tcagccacat atttatggtt aagcaaattg tcggcataaa
     1261 tcgagatacc gatattcttc catttataat gcacatctgc cgaaaaaacc gtgtatgccg
     1321 gcagggaacg ctcatcggat gaggtattgt catagtactg tttgccaaca tatttgccac
     1381 gcaaagaaaa agagagcttg tccagagggt tccatgtagc catagccata ccaacgatcg
     1441 gcggggacat caggatctcc ccattttcaa cacgctgcat agcctgtggc aggggattcc
     1501 attcgtccgg attgtcatat ctatccaccc ataggttgca ctccgtgatg cggttgcgac
     1561 tcaaagctat attagcatca aacctcagac tccgcaaagc ctgccagcct gccgagagtt
     1621 cgatgcctcg tctgtagcta cgctccacat tctccttgac tctgtatccc acttctgtga
     1681 ttttaccggt ctcaagaagc tgattacgat actccataag gtagagattt gccgaaaaag
     1741 caaacagagg cgaagtataa ttgtagccaa actcccaatc catcattcgt tccggcaaga
     1801 tcctctcccc ctctcccgcc ttgatggcct ccttgatatc acttcgcgaa ggctccttgt
     1861 gggccaccgc caacgatgca taagcagccg aacggccgtc aaaattccag gatactcccc
     1921 ctttgggatt gaagaaatta tagataaaat ctttatccaa agcgacgaaa tccttatcaa
     1981 tacccgaaag cgcataattt acattacgat attgtatatc accatagaga tgcaactttc
     2041 caccgaaaac atcaccttct ccccgcaaga agccggaaag atcctttttg agaccccggt
     2101 tgaggtacca tctccaatcc tcaggaatat tctcattgta ttttgcccaa atcacatttc
     2161 cgtagtggtc accgtcgtag agcgaccagg agaggttgcc cagcagaaaa aatccctctc
     2221 cggagtactt tagattggta gtagcagcaa aattgttgtt ctccatctgc tggcgtacaa
     2281 caacatcgct tcgtttatag cggactccgc ctaccagctg ctccgataat ccgtaatcgg
     2341 aaaatttctt accggcttta taattttcat aataacctgc tccacgggta taattaaaag
     2401 tggcactcat agagaggtgc ggagaaaatt gatggaccca gatggcttga agatgatttt
     2461 gtaaatagtt gtcggtttca ttatcataat agagtacatt tcccgaccca tcgctatact
     2521 ctcctgcaga attgtacctg cggtcactct ccagcatatc cgccggaatg cccaaccagg
     2581 ttataccgct tcgctgtttg cccataatcc agttgagccg gaaggagtcg gagctacgca
     2641 accagcccaa agctgcataa gccgagtgga gccttgcaaa ggcattcctt atatagccct
     2701 cagtggtatt gtacgagtag ttcatatcga gcgatagccc gctcttacct cgtcccgtac
     2761 cggcaccgac ggtggtactg ccggtgagcc acgaacctcc tgagagggcg accttggcat
     2821 aagggttttc agcggcccca agggtgctca tattcagagt cgctccgaag gcaccagccc
     2881 cgttgaccga agtacctgcg cccctctgta attgtacaga ttcgagaaat cccgcaatag
     2941 cgggaatatt gacccagaag accttctgcg actcagcatc attgagcgtg ataccgttga
     3001 tgttgacatt gatgcgggta gcatcactcc ccctgaccct catttgggag tagcccaggc
     3061 cggtcccccc ttcgttggtg gccaccacgg agggctgtag tgagaggagc atgggcagtg
     3121 aggtcattgt acccgctctc gaaagttgct ctctcttgat ctcggtggat gtgacaggtg
     3181 tccccgattg cgcccttgat gcagtcacaa tggtactgtc gatagtgtaa tggggatgtg
     3241 attggtgtaa atccggtgca ctgttcagtg atgattgttg tgcagataag agtgcacaaa
     3301 gcagcagaaa tgctgctgat aacaatgttt ttttcataaa aatcctc
//
LOCUS       DAOSIE010000159         3346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_24834_length_3346_cov_3.900030, whole genome shotgun sequence.
ACCESSION   DAOSIE010000159 DAOSIE010000000
VERSION     DAOSIE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24834_length_3346_cov_3.900030"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /gene="nifJ"
                     /locus_tag="PLI69_05220"
     CDS             complement(<1..1542)
                     /gene="nifJ"
                     /locus_tag="PLI69_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOG41920.1"
                     /translation="MAKEKKFITCDGNYAAAHVAYLFSEHAAIYPITPSSTMAEYVDE
                     WAAYGKKNMWGEIVKVTEMQSEGGAAGAVHGSLQAGSLTTTFTASQGLLLMIPNMFKI
                     SGELLPAVFHVSARTLAAQALSIFGDHSDVMSARQTGFAFIASASVQEAMDMAAVAHL
                     SAIKSSIPFVHFFDGFRTSHEIQKVELLEAEDLRPLVSDKSLAKFRERALNPNKPVTR
                     GTAQNPDVYFQAREASNPYYDAVPDIVARYMGEITELTGRHYLPFDYYGAPDAENVII
                     AMGSVCETIKETIDYLAKTKGEKLGMIAVRLYRPFSAKYFLRVLPKSVKRIAVLDRTK
                     EPGSLGEPLYMDVKAIVADLGENAPLVVGGRYGLSSKDTSPAQIVAVFDNLKLREPKN
                     GFTIGIVDDVTFKSLPLKEEISLAKKGTYECKFYGLGSDGTVGANKNTIKIIGDNTSK
                     YCQGYFDYDSKKSGGYTCSHLRFGDSPIQAPYLVSTPDFVACHVPSYLYKYDVLKGIK
                     EGGILL"
     gene            1729..2409
                     /locus_tag="PLI69_05225"
     CDS             1729..2409
                     /locus_tag="PLI69_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41921.1"
                     /translation="MKRILMIILTACFALSTQAQNVIQHKKGEDMTQHMLDSVKYVLP
                     DFTAGIVIFKDGRTANGPLNISTISQKLLFISPQGEIQEVLNQNNIDRVSVKGRSFVQ
                     TKQGFVELLDIADNVILGSVKRVKFLESEKKGAFGTTSETTSVTSISSISTEGQRYEL
                     AQHVNTPFTYRVTPYLGINGKFSYATKKYILKCFPSKKDKIESYLKENKVDFESLEDV
                     RALFEYLK"
     gene            complement(2431..3306)
                     /gene="fabD"
                     /locus_tag="PLI69_05230"
     CDS             complement(2431..3306)
                     /gene="fabD"
                     /locus_tag="PLI69_05230"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOG41922.1"
                     /translation="MKAYVFPGQGSQYPGMGKELYSTSPRAKALFEQANQILGFRITD
                     IMFEGDAEALRATKVTQPAVFLHSVVGALCSDLPTPDMVAGHSLGEFSALVVSGALSF
                     EDGLRLVALRAQAMQKACDLNSGSMAAIIGADSDLLERICRETPGIVVPANYNSDQQT
                     VISGETPAVESVVNALKEAGVRKCVMLQVGGAFHSPLIEPARVELAGAIDKTPFSIPL
                     CPIYQNVDALPNTDPDRIKSNLLMQLTSPVRWLDIMLNMLADGATQFTEIGPGNVLQG
                     LIKKTAPDVELFSIG"
BASE COUNT          925 a          750 c          763 g          908 t
ORIGIN      
        1 aagaaggatt ccaccctctt tgatgccttt tagaacatca tatttgtaga gatatgaagg
       61 tacgtggcag gccacgaaat cgggagttga tacaaggtaa ggggcctgga tggggctgtc
      121 gccaaatctc aggtgagagc aggtataacc gcctgacttc ttggagtcat agtcgaaata
      181 accctggcaa tatttggagg tgttgtcacc aataatttta atggtattct tgttggcacc
      241 aacagtaccg tctgaaccca gaccgtagaa tttacactcg taagtgcctt ttttggctag
      301 agatatctcc tctttgaggg ggagtgactt gaaggtaaca tcgtctacta taccgatggt
      361 aaagccgttt ttgggctctc tgagtttgag gttgtcgaat actgcgacaa tctgggcggg
      421 actggtatcc tttgaagaga gaccgtagcg accgcctaca accaatggcg cattttctcc
      481 gagatcggca acaatggctt ttacatccat atagaggggc tcaccaagtg atccgggttc
      541 tttggtacga tcaagtactg caatcctctt tacactctta gggagtactc tgaggaagta
      601 ttttgctgag aaaggtctgt aaagacgcac tgcaatcata ccgagtttct ctcccttggt
      661 ctttgccagg taatcgatag tttcctttat ggtttcgcat acggaaccca ttgctataat
      721 tacattctct gcatctggtg caccatagta gtcaaagggg aggtaatggc gacctgtaag
      781 ttcggttatt tcacccatat atcttgctac gatgtcggga actgcatcat agtatggatt
      841 cgaggcttca cgtgcctgga aataaacgtc agggttttgt gccgtacctc tggtaacggg
      901 tttattggga ttcaaagccc tttctctgaa tttagcaaga gatttatcgc ttaccagcgg
      961 cctgagatct tctgcctcga gcaactctac tttctgtatc tcgtgtgagg tcctgaagcc
     1021 gtcgaagaag tgtacgaacg ggatgcttga cttgatggct gaaagatggg ctacagcagc
     1081 catatccatt gcctcttgta cactggcaga tgcaatgaat gcaaatcccg tctgtctggc
     1141 actcataacg tcagaatgat caccaaaaat ggatagagcc tgtgcagcaa gtgtgcgggc
     1201 agatacatga aatactgccg gaagaagttc gcctgatatc ttgaacatat tggggatcat
     1261 caggagaagg ccttgagagg ctgtaaatgt tgtagtcagg ctacctgcct gaagggatcc
     1321 gtgaacagct cctgctgcgc caccttcact ctgcatctca gtaactttaa ctatttcacc
     1381 ccacatattc tttttgccgt aggctgccca ctcatcaacg tactctgcca ttgtcgaaga
     1441 gggagtgata gggtaaatag cggcgtgctc actgaaaagg taagcaacat gggcggcagc
     1501 ataattgccg tcacaggtga tgaatttttt ctcttttgcc atttgattaa tgttttttat
     1561 tgatttgtcc cgtttttgtt attaatcgta actttgaaaa ggactttatc ttaattatac
     1621 ctatttttat tagtaacaaa actcaaaaat atggattatt tttgggataa gaaaatttaa
     1681 tgtctatttt tacttttcaa acagattgtt atatcaattt tcaaaattat gaaacgcata
     1741 ttgatgatta ttctcaccgc ctgttttgct ttgagtaccc aagctcagaa tgtaatccag
     1801 cacaaaaaag gtgaagacat gacccagcat atgcttgaca gcgtcaagta tgtcctaccc
     1861 gatttcacag cgggaatagt tatattcaaa gatgggcgta cggccaatgg cccgctcaat
     1921 atcagcacca tctcccagaa actcctcttt atctccccac aaggtgagat acaggaagta
     1981 ttgaatcaga acaacattga ccgcgtatcg gtcaaagggc gctcttttgt ccagacaaaa
     2041 cagggatttg tagagttgct cgacatagca gacaatgtaa tattaggaag tgtaaagagg
     2101 gtcaaattcc ttgagtcgga gaaaaagggt gcattcggta ctacatccga gaccacgtca
     2161 gtcacctcca tcagctccat ctccacagag ggacaaagat atgaacttgc ccaacatgta
     2221 aatactccct tcacttacag agtgaccccc tatcttggga tcaacggtaa attcagttat
     2281 gctacaaaga agtatatcct gaagtgtttc ccatccaaaa aggataaaat agagtcctat
     2341 ctcaaagaaa ataaagtgga ctttgagagt ttagaggatg tgagagccct atttgagtat
     2401 ctcaaataga ggtcaataca acgctactat ttagccaatt gaaaacagct ccacatccgg
     2461 ggctgttttt ttgatcaggc cctgaagcac attaccgggg ccaatttcgg taaattgagt
     2521 cgccccgtct gccaacatgt tgagcattat atccagccat ctgaccggag aggtgagctg
     2581 catcagcaga ttggatttga tccgatccgg atcagtatta ggcagagcat ccacattctg
     2641 ataaatcggg cagagtggta tcgaaaaggg agttttatct attgctccgg caagttccac
     2701 acgtgccggc tcaataagag gggagtgaaa agcaccacct acctgaagca tcacacactt
     2761 tcgtacacct gcctccttga gggcattgac tacactctcc acagcgggag tctcgccgga
     2821 aataaccgtc tgctgatcgc tattgtagtt tgcaggtacg acgatgcccg gggtttctct
     2881 acagattcgc tccagaagat ccgagtcagc gccaatgatg gcagccatcg agccggaatt
     2941 gaggtcacat gctttctgca tagcttgcgc ccttagagca acaaggcgta aaccatcttc
     3001 aaaacttaga gcacctgaga caacaagtgc cgaaaattcg cctaaagagt gacccgccac
     3061 catatccgga gtgggcagat cactgcaaag cgctccaact accgagtgaa gaaaaaccgc
     3121 aggctgggta actttcgtag cccttagggc ctctgcatct ccctcaaaca taatatcggt
     3181 aattctgaat ccgagtatct gatttgcctg ctcaaaaaga gcttttgctc ttggagaagt
     3241 gctataaagt tccttcccca ttcccgggta ttgtgatccc tgaccgggaa aaacgtatgc
     3301 tttcatctta ataaggtttt agtaacggcg gcaaagatag aaaaaa
//
LOCUS       DAOSIE010000160         3343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_24856_length_3343_cov_3.573601, whole genome shotgun sequence.
ACCESSION   DAOSIE010000160 DAOSIE010000000
VERSION     DAOSIE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24856_length_3343_cov_3.573601"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /gene="scpA"
                     /locus_tag="PLI69_05235"
     CDS             complement(<1..1488)
                     /gene="scpA"
                     /locus_tag="PLI69_05235"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOG41923.1"
                     /translation="MRPNFKDIKYTGAPSECCSQEKTTSWNTPEKIEVKSIYTEKDLE
                     GMEHLGYAAGIPPYLHGPYSTMYVMRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLS
                     VAFDLATHRGYDADHPRVVGDVGKAGVSICSVEDMKVLFDQIPLGKMSVSMTMNGAVL
                     PILAFYIVAGLEQGVKLEEMAGTIQNDILKEFMVRNTYIYPPEFSMRIVGDIFAYTSR
                     FMPKFNSISISGYHMQEAGATSDIEMAYTLADGLEYLRTGIKAGIDVDAFAPRLSFFW
                     AIGMNHFMEIAKMRAARMIWAKLVNQFNPKDPKSLSLRTHCQTSGWSLTEQDPFNNLA
                     RTCIEAMGAVLGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQEETNVCRSIDPWA
                     GSYYVESLTKEIAHKAWALIEEVEKLGGMAKAIETGLPKLRIEEAAARKQARIDSGME
                     KIIGVNQYRLDKEDPIEILDVDNTKVRESQIKRLKELRANRDEAKV"
     gene            complement(1501..>3343)
                     /locus_tag="PLI69_05240"
     CDS             complement(1501..>3343)
                     /locus_tag="PLI69_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOG41924.1"
                     /translation="VIIKDLKGADYEKRLVWKTHEGFNVRPYYRAEDLRNLRYIGSKP
                     GCFPFVRGTKKENQWYIHQGICACDGNLAKANSEAKELLTKGVESLGFYLDTRKVQTK
                     EDFALLLGGIDLTVVPVSFCGCSLQNVETLQNFIAYVDSTGVDKDAVNAIFDFNPLMT
                     LTTKGYFCEEASFEKLAECVKAAQEYKNIRVITVDATTFNGAGASSTQELAFALAEGS
                     EYIARLKEFGLSVKDIAKKMVFVFSVGSSYFMEIAKFRAARMLWANVVEAYGTDSTCA
                     KKMTIFAYTGEWNQTVYDSYVNMLRATTEAMSAALAGVDYLEVVPFDFAYQVPGEFSN
                     RIARNVQSILKEESNFDKVVDPAAGSYYIENLTNSLAEAAWKLFTDVESAGGYVVKFK
                     EGFIQSEIKAVSDKKDKNIATRREILLGTNQYPNFLEVADEVITKEIVSREIPTLMGM
                     KMEESCCCREKIAEPIIPYRGSQAFEKLRLATDRSGKEPKAFMLTFGNLAMCRARAQF
                     SSNFFAVAGIKVIDNNRFDTIEEGAKAALESGAEIVVACSSDEEYAEAVPQIAKLLGG
                     KAILVVAGDPACREELVAAGINNFINVKSNVLETLKEYQKMLGIVEL"
BASE COUNT          849 a          831 c          728 g          935 t
ORIGIN      
        1 tacctttgcc tcgtccctat tggcacggag ttctttgagt ctcttaatct gtgactcgcg
       61 aaccttggta ttgtccacgt cgagaatctc gataggatcc tccttatcaa gtctgtactg
      121 attgacacct atgatcttct ccatacccga gtcgatgcgc gcctgtttac gtgcagctgc
      181 ctcttctatg cgaagtttgg gtaggccggt ctcaatggct tttgccatac cgccaagttt
      241 ctccacctcc tctatgagag cccaggcttt gtgcgctatc tccttagtca ggctctcaac
      301 ataatatgaa cctgcccagg gatcaatgga gcgacatacg ttggtctcct cctggatata
      361 gatctgagtg ttacgtgcaa tacgtgcaga gaaatctgtc ggaagcgcta tcgcctcatc
      421 aagagcattg gtgtggagtg actgggtgtg tccaagtact gcacccatag cctcaataca
      481 ggttctggca agattattga atggatcctg ctctgtgagg gaccaacctg aagtctgaca
      541 gtgggtcctc aaagagagag atttgggatc tttcggattg aactggttta ccagcttagc
      601 ccaaatcata cgtgccgcac gcatctttgc tatctccata aagtgattca tgccgattgc
      661 ccagaagaat gagagacggg gagcaaatgc atcaacgtcg atacctgcct tgatacctgt
      721 gcgaaggtac tcaagaccgt cggcaagagt gtatgccatc tcaatgtcac ttgtagcacc
      781 tgcctcctgc atgtggtagc cggagatgga gatggagttg aacttaggca tgaacctgga
      841 ggtataggcg aaaatatctc ccacaatcct catggagaac tcggggggat aaatataagt
      901 attgcgcacc atgaactcct tgaggatgtc gttctgtatg gttcctgcca tctcttccag
      961 tttcacccct tgctccagac ctgctacaat gtaaaaggcc agaatgggta gaaccgcacc
     1021 gttcattgtc attgatactg acatcttgcc aagaggaatc tggtcaaaga gaactttcat
     1081 atcctctacc gagcagatac ttactcctgc tttacccaca tcacctacaa ctctcggatg
     1141 gtctgcatca tatccacggt gagtagccag gtcaaatgcc actgaaagtc ctttctgacc
     1201 tgctgcaaga ttacgtctat agaaagcgtt ggactcttct gcagttgaaa aaccggcata
     1261 ttgtcggatt gtccatggcc tcattacata cattgtggag taaggtccat ggaggtaagg
     1321 agggataccg gcagcatagc caaggtgctc catcccttca agatcctttt ccgtatagat
     1381 tgatttcact tctatctttt ccggagtatt ccaggaagtt gttttctctt gactgcaaca
     1441 ttctgaaggg gctccggtat atttgatatc tttaaaattc ggtctcatat ctttcagttt
     1501 ttatagttct acgataccaa gcatcttttg atactctttc aaagtctcaa gaacattgct
     1561 ttttacatta atgaaattgt tgattccggc agcaacaagc tcctctctgc aagccggatc
     1621 gcctgcaaca accaggatgg ccttgccacc aaggagcttt gcaatttgag gtactgcttc
     1681 cgcatactct tcatcggaag aacaggcaac cacaatctcg gctccggact cgagagcagc
     1741 tttagctccc tcttctatgg tatcgaaacg gttgttgtca ataaccttta tacctgctac
     1801 cgcaaagaag ttggatgaga actgtgctct ggcacggcac atggcaaggt tgccgaatgt
     1861 aagcataaat gccttgggct ccttgccgct acgatccgta gccagacgaa gtttctcgaa
     1921 tgcctgtgag cctctgtagg gtattatagg ctctgcaatc ttctcacgac agcagcaaga
     1981 ttcttccatc ttcataccca taagtgtcgg aatctctctg gaaactatct cctttgtaat
     2041 cacctcgtcg gcaacctcta ggaaattggg gtactggtta gttccgagga gaatttccct
     2101 gcgggtggct atatttttat ctttcttatc ggaaacagcc ttgatctcag actgtatgaa
     2161 gccctctttg aactttacca cataaccgcc ggcactttcc acatcggtga agagtttcca
     2221 ggcagcttca gctaaagagt ttgtaagatt ttcaatataa tatgaaccgg cagccgggtc
     2281 gacaacctta tcaaaatttg actcttcctt cagtatgctc tggacattgc gtgcaatcct
     2341 gttggaaaac tctcccggca cctgatatgc aaagtcaaag ggcactacct ccagataatc
     2401 tactcctgca agagcagcgc tcatcgcctc cgtagttgcg cgaagcatat ttacatagga
     2461 gtcgtataca gtctgattcc actcgccggt gtaggcaaaa atcgtcatct tcttagcgca
     2521 ggtagagtca gtaccataag cctccacgac atttgcccaa agcatacgtg ctgcgcggaa
     2581 cttcgcgatc tccataaagt atgaactgcc cactgagaag acgaaaacca tcttctttgc
     2641 aatatccttg acagatagtc cgaactcttt cagacgtgca atatattcgc tgccttcagc
     2701 aagagcaaat gcgagttcct gagtagaaga ggcaccggct ccgttgaaag tagtggcatc
     2761 aactgttatg acgcgtatat ttttgtattc ctgcgctgct ttcacacatt ctgcaagttt
     2821 ttcaaaagaa gcctcctcgc agaaataacc cttagtggta agtgtcatca aagggttaaa
     2881 gtcgaaaata gcattgaccg catccttatc gactcctgtg gaatctacat aagcgataaa
     2941 gttttgtaga gtttcaacat tctgaagtga gcatccgcaa aatgatacgg gtacaaccgt
     3001 caggtcaata ccacctagaa gcagtgcaaa atcctctttg gtctgaacct ttcttgtgtc
     3061 gagataaaaa ccgagggatt cgacgccttt tgtcagaagc tcctttgctt cagaattagc
     3121 ttttgcaaga ttgccgtcgc aagcacagat accctggtgg atataccact gattctcctt
     3181 ttttgtacct ctgacaaatg ggaaacaacc gggtttacta cctatgtacc tcaaattccg
     3241 tagatcttct gcacggtagt aaggacgtac gttgaaccct tcgtgagtct tccaaacaag
     3301 tcgcttctca tagtcggctc cctttaagtc cttgataatt acc
//
LOCUS       DAOSIE010000161         3260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_25713_length_3260_cov_2.754134, whole genome shotgun sequence.
ACCESSION   DAOSIE010000161 DAOSIE010000000
VERSION     DAOSIE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25713_length_3260_cov_2.754134"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PLI69_05245"
     CDS             complement(<1..1033)
                     /locus_tag="PLI69_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41925.1"
                     /translation="MKEKEKTILGCMSCIIEDIRDTEKKFNEKIFEEKAEEIEYISTM
                     CGTTPWQSVLLSCIIERSNRNRLDKSDLARFMGMSYIKLLAFDTDLASLHKMRLIVVY
                     SDSYIHLPSHVLSSLSKNQPYSIPDNYNLDTPELMKRLRDLLKQRMDDELDEWDMVER
                     LDELMANNQECSFVKAAAKYRIFIDGNLDLCQQEKVVFYNLVYRYLYEDDDQVGWHDF
                     NDVFQDSSDINVMRSRYRREGLLLQLRGIIEPVGEDGFLNVDLFHIKDEIKEELFEDV
                     GGLRKRTTRKTRMKLIPSKDIKPRDLFYNPEEAEQVNRLASLLADSTYRSTCERLKES
                     GLRTGFSCIF"
     gene            1190..2110
                     /locus_tag="PLI69_05250"
     CDS             1190..2110
                     /locus_tag="PLI69_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOG41926.1"
                     /translation="MAEKSRVYSRYKWLVDIIQRNDGITFEEIDRAWQHSSLNTVPYS
                     PLTARTFHRHKREIEEVFGIIIKCNKSSNKYYIDNEDEVEEGKIQEWMLSTIAVDNML
                     MESRDLRDRIQFEDIPGGRRFLDVVINAMRGNKVLHMIYQSFWSDEHQDTWLRPYFLK
                     IFQQRWYVVGVPGTHPNTLRTYALDRMVSLEITPEKFKYPKRFNPSAYYANCYGIFHQ
                     EGEPEKIILKVQDDQVKYFDSLKVHHSQSKRNDLKEDGYTYYEYRIAPAYDFVQFLCS
                     KCSSVEVIEPLELRHWVAEELKKGIEKYKG"
     gene            2495..>3260
                     /gene="dcm"
                     /locus_tag="PLI69_05255"
     CDS             2495..>3260
                     /gene="dcm"
                     /locus_tag="PLI69_05255"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR00675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HOG41927.1"
                     /translation="MDNNKKYNVLDLFCGCGGISEGFRMAGYNIIGGIDIDKDATDTF
                     AKNFIAAKVICSDITKIINEEINSYYHNVDIIVGGPPCQGFSSANRWQKERDDPRNKL
                     FFEYLRFVEVLRPKVVLIENVQGLLTRDNGYAKKRITSLLNDMNYSISMDVLNASDYG
                     VPQNRKRAIIIAINTDFCTTQFDFDKIVKLPKVTIDEALSDIYGFEKQPGKKTYKQTK
                     AKSAYQELMKNGTKVLFDHEVKYPAEIVQKRISFVPQ"
BASE COUNT         1006 a          676 c          669 g          909 t
ORIGIN      
        1 aaaatatgca tgagaaaccg gtgcgtaggc ccgactcttt aaggcgctcg caggtggaac
       61 ggtaagttga atccgccaga agagaggcca gccggtttac ctgttcggct tcctccggat
      121 tatagaaaag atccctgggc ttgatgtcct tgcttggaat caacttcatt ctcgtcttcc
      181 ttgtcgtcct cttcctcaat ccacccacat cttcgaaaag ttcctcctta atctcgtctt
      241 taatatggaa taaatcgaca tttaaaaagc catcctcccc gacgggctcg atgatgccgc
      301 gtaattggag caacagacct tctctcctat atctgcttct cattacattg atatccgaac
      361 tgtcctgaaa tacatcattg aagtcatgcc aaccgacctg atcatcatct tcgtagagat
      421 aacgatagac aaggttgtag aacaccactt tttcttgttg gcataaatca agattgccat
      481 ctataaaaat acgatatttt gcagccgcct tgacaaaaga gcactcctga ttattcgcca
      541 tcaattcatc aagcctctct accatatccc attcatccaa ctcatcatcc atccgctgct
      601 ttaaaaggtc acgcagccgc ttcataagct ccggagtgtc gagattataa ttgtcgggaa
      661 tggagtatgg ttggttttta gagagagatg agagaacgtg agagggaaga tggatataac
      721 tatcggaata cacgacgatg agtctcattt tgtgcaggga agcgaggtct gtgtcgaagg
      781 cgagcagttt gatgtagctc atccccataa atctggcaag atccgacttg tcgagtctat
      841 ttctattact cctttctata atgcacgaga gcaatacaga ctgccaggga gtcgtgccgc
      901 acattgtgct gatgtactct atttcttccg ccttctcctc aaaaatcttt tcgttgaatt
      961 ttttttccgt gtcacggatg tcttcaataa tgcagctcat gcagccgagg attgtctttt
     1021 ctttttcttt catggtaatt ctcccttttt tctaccgcta atatatatca cacatatgcc
     1081 atttcgtggc atagctgtaa aaaaagaaca aaataatttt tatcgtgccg ttttttgtca
     1141 ctgcaatttc ataactttgt aagcataaaa gaattaacgg caataaatta tggcagaaaa
     1201 atcccgcgtt tactcaagat acaaatggct ggtcgacatc atccagcgca atgacggcat
     1261 cactttcgag gaaatagacc gcgcatggca acattcttcc ctgaacacgg ttccttactc
     1321 tccgctcact gcgaggacct tccatcgcca caaaagggaa atcgaggaag tcttcggcat
     1381 catcatcaaa tgcaataaaa gctcaaataa atattatata gataatgaag atgaagtgga
     1441 ggaggggaag atacaggagt ggatgctttc gacaatagca gtggacaata tgctgatgga
     1501 gtcccgcgac cttcgcgacc gcattcaatt cgaggacatt ccgggcgggc gccggttctt
     1561 ggatgtagtc atcaatgcga tgcggggcaa caaggtcctg catatgattt accaaagctt
     1621 ctggagcgat gaacatcagg acacctggct tcgtccgtat ttcctgaaaa tcttccagca
     1681 gagatggtat gttgtgggcg ttcctggcac acatccgaat actctacgca catacgcatt
     1741 ggacagaatg gtttcattag agataactcc ggaaaagttc aaatacccga aaaggttcaa
     1801 cccttccgcc tactacgcaa actgctacgg cattttccat caggaagggg agcccgagaa
     1861 gattatactc aaggttcaag acgatcaggt aaaatacttt gattccttga aggtccatca
     1921 ttcgcaatcc aagcggaacg atttgaaaga agatggctac acttactacg aataccgcat
     1981 cgctcccgcg tatgacttcg tgcaattcct ctgctcaaaa tgctcatccg tggaggtgat
     2041 agagcctctt gagctcaggc actgggttgc cgaagagctg aaaaagggga ttgagaaata
     2101 taagggttga atttgaggtg gataaggggg ggggatgtaa gcaatggcaa taatccggaa
     2161 attgtcgcca gtcacactgc ttccgtgcaa aaaaattacg gggcaagcat aactaagcgt
     2221 gatacattag tatctaccat acgtcactta ttctttttca aggagagcga ctacttcggc
     2281 ttcaagcctt gagtatatgc ctagctaatc atctctaacc atttcaaatt attcgcccgt
     2341 cataatatta caatatagtt atttttatta aatctaccgt acaacatggc aaaccaaact
     2401 gaattaagcc tatggtttgc ataaactcaa tcttataaga ttggataata tttattaaat
     2461 ttgtaaattc aaacttatag cataagtaag tagaatggac aataacaaaa aatataatgt
     2521 tttagatctt ttttgtggat gtggtggaat cagcgaagga ttcagaatgg cgggatataa
     2581 tattatcgga ggtattgata ttgataaaga tgccacagat actttcgcca agaattttat
     2641 tgcagcaaaa gtgatatgta gtgacataac aaaaataata aatgaagaaa taaatagtta
     2701 ctatcataat gtagatataa ttgtcggcgg gccaccatgt caaggtttct cttctgcaaa
     2761 tcgttggcaa aaagagagag atgatcctcg caacaaattg ttttttgaat atttacgttt
     2821 tgtggaagtg ttacgtccaa aggttgtctt aattgagaat gttcagggac ttctaaccag
     2881 ggataatgga tacgcaaaaa agagaatcac atcactttta aatgacatga actattccat
     2941 cagtatggat gtgcttaatg catcggacta tggcgtaccg caaaacagaa aaagagcaat
     3001 tattattgca ataaacaccg atttttgtac aactcaattc gactttgata aaattgttaa
     3061 attgcctaaa gttaccatag atgaggcctt aagcgacata tatggttttg aaaaacaacc
     3121 tggcaaaaaa acatacaaac aaacaaaagc aaaatccgcc tatcaggaat taatgaaaaa
     3181 cggaacaaaa gttctttttg atcatgaggt aaagtatcct gctgagattg tacagaaacg
     3241 aataagtttt gtgcctcaag
//
LOCUS       DAOSIE010000162         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_26043_length_3227_cov_3.437894, whole genome shotgun sequence.
ACCESSION   DAOSIE010000162 DAOSIE010000000
VERSION     DAOSIE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26043_length_3227_cov_3.437894"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLI69_05260"
     CDS             <1..747
                     /locus_tag="PLI69_05260"
                     /inference="COORDINATES: protein motif:HMM:NF020416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG41928.1"
                     /translation="GVGSSEPGNPDATSFDLSYDAEAAFDYLLTRKEVIPSLCGITGH
                     SEGGFINFMVAQRRPDVAFLISLGGPAVTGAEVLKLQQVEVYRGMGMPEQAIEQNRVV
                     SEQVFAIVEKHDAPDENFRKEALSYLASLGMPDDVAVPMIDQYSSPWMFYFLKYDPAP
                     AIREVTVPALILNGTLDKQVVSYQNIPVFRQIAEETGRDNMQIVELEGLNHLFQHATT
                     GLPTEYATIEETISEEVLALMLDFVKTIKK"
     gene            744..1169
                     /locus_tag="PLI69_05265"
     CDS             744..1169
                     /locus_tag="PLI69_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41929.1"
                     /translation="MTIAIDFDGTIVEHAYPEIGRPIPFAIETLLQMQKDGHRLILWT
                     VRTGDLLQEAIDYCAKKGLHFYAENENYRGETEELQPGEYHRKLDADMFIDDKSLGGL
                     PDWGVIYNAVKVTLDGGDALETMASGSAMPEQKKRRWFR"
     gene            1358..2671
                     /locus_tag="PLI69_05270"
     CDS             1358..2671
                     /locus_tag="PLI69_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOG41930.1"
                     /translation="MKQQLLNKISRKEITVGVVGLGYVGLPLAVEKAKAGFKTIGFDV
                     QRQKVDMVNAGHNYIGDVVDSDLEEIVSKGLLSATSDFSFIKDVDFVAICVPTPLDIY
                     QQPDISYVKSSTEAISKYMKRGTMVVLESTTYPGTTEELIKPILEEGSGLKCGEDFYL
                     GFSPERVDPGNLIYKTKNTPKVVGAIGEDALEVIAAMYRAVLESDVHTVSSPAIAEME
                     KILENTYRNINIGLVNELAILCHKMNINLWEVIDAAKTKPYGFQAFYPGPGLGGHCIP
                     LDPYYLSWKAREFGFHTSMIESSMMINDRMPEYCTERAAHILNRYKKSLNGSDILVLG
                     VAYKQDIDDYRESPALVVIDILKGLGANIKYYDPYITEYIYKGEKHQGENALTKELLE
                     AADLVIVTAAHTKVDYNFVQQHSKFIFDTKNAMKAVVNRENIELL"
     gene            2719..>3227
                     /locus_tag="PLI69_05275"
     CDS             2719..>3227
                     /locus_tag="PLI69_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG41931.1"
                     /translation="MKRICVIGGGKWGQNHIRTLFEMGNLGGIVESNPKRLKELLEKY
                     PVKGFQDVEAAVKENFDGFTIAAPAPLHYEIAKLLIQNRKNVLVEKPMTLSSETSLEL
                     VELAKAKGVRLMVGHVLLFHPAIRKIKEVIDDGKIGELYYIYSNRLNLGTVRTEENVF
                     WSFAPHDVSV"
BASE COUNT          951 a          637 c          770 g          869 t
ORIGIN      
        1 ggagtaggtt catccgagcc cggcaatccc gatgctacct cctttgacct ttcctatgat
       61 gccgaggcgg catttgacta tctgcttacc agaaaagagg taattccttc actttgcggc
      121 attaccggcc atagcgaagg gggttttata aacttcatgg tggctcaaag gcggcctgac
      181 gttgcttttc ttatttcact cggaggtccg gcagtgacag gtgcagaggt tctcaaactc
      241 cagcaggttg aagtatatcg aggaatgggt atgcccgagc aggccatcga gcaaaacaga
      301 gtggtctcag agcaagtttt cgccatagtg gagaaacatg atgctccgga tgagaacttc
      361 cgcaaagaag cgctgagtta tcttgcgtct ctgggaatgc ctgatgatgt cgcagttcct
      421 atgattgacc aatacagctc tccctggatg ttttatttcc tcaaatatga tccggccccg
      481 gcaattcgag aagttaccgt accggcattg attctcaacg gcacgcttga taaacaggtg
      541 gtatcatatc agaatattcc tgttttccgt caaatagctg aagagacagg cagagataat
      601 atgcagatcg tggagctcga gggactcaat catctcttcc agcatgctac cacaggtctc
      661 cctactgaat atgccactat agaggaaacc atttcggaag aggtgctggc gctgatgttg
      721 gattttgtaa aaactattaa gaaatgacca tagctattga ctttgacggc actattgtgg
      781 agcacgccta tccggaaatt ggcaggccta ttcctttcgc catagaaaca ctattgcaga
      841 tgcagaaaga tgggcacagg cttatcctat ggactgttcg gaccggtgac ctcctgcaag
      901 aggctataga ttactgtgct aagaagggat tgcacttcta tgcagaaaac gaaaactaca
      961 ggggagaaac ggaggagttg caaccgggtg agtaccatcg taagttggat gctgatatgt
     1021 ttatagatga caagagctta ggaggattgc ctgactgggg ggttatctat aatgcagtaa
     1081 aggtcacgct ggatggaggt gatgcgcttg aaactatggc atccggttct gcaatgccgg
     1141 agcagaagaa aaggcgttgg tttaggtaac ggtttccaaa aaaaaaatat cgacaattta
     1201 acatttctgc cactctattt tgtaaaaaga caaaatagac taccttgttg ttcgtttttt
     1261 ttagatttca agtatttact tcaaagattt gttgtatatt tgcatgatac aatgttagta
     1321 ataatatcac gggaactaaa tcttaaacat gtagaaaatg aagcagcaat tattaaacaa
     1381 gatttcaaga aaagagatta cagtcggcgt agtggggcta ggttatgtcg gactaccttt
     1441 ggccgtagaa aaggcaaaag ccggttttaa aacaatagga tttgacgttc aacgccaaaa
     1501 ggttgatatg gtaaatgccg gacacaacta catcggcgat gtagtagata gtgatctgga
     1561 ggagattgtt tcaaagggat tgctatcggc cacttccgat ttcagtttca ttaaagatgt
     1621 cgattttgtg gctatctgcg tacctacccc tcttgatatt tatcagcaac ccgatattag
     1681 ttatgtcaag tcttctactg aagctatttc caaatatatg aagagaggga cgatggtggt
     1741 attagaatcc actacctatc ctggcacaac agaggagctg ataaaaccca ttcttgaaga
     1801 gggctccgga ttaaagtgcg gtgaagattt ttatctcggt ttttcgcccg aaagagttga
     1861 ccccggcaat ctgatttaca aaaccaaaaa tacgccaaag gtagttggag caatcggaga
     1921 ggatgcgctt gaagtaattg cagccatgta tagagcggtt ctcgaaagcg atgtgcatac
     1981 ggtttcgagt cctgcgattg ccgaaatgga gaaaattctc gaaaacacct atcgtaatat
     2041 aaatataggg ttggtcaacg agttggctat actctgtcat aaaatgaata tcaacctttg
     2101 ggaggtaatt gacgcagcca aaacaaaacc ctacggattt caggccttct acccgggtcc
     2161 cggattgggt ggtcactgta ttcccctcga cccttattat ctctcatgga aagcacgtga
     2221 gtttggattc catacctcta tgatcgagtc ttcgatgatg atcaacgacc gtatgcccga
     2281 gtattgtacg gagagagccg cccatattct caacagatat aaaaaatcgt tgaatggctc
     2341 cgatatactt gttctaggtg tagcttataa gcaggatatt gatgattatc gtgaaagccc
     2401 cgccttggtg gttatcgaca ttttaaaggg gctgggagca aacataaaat attacgaccc
     2461 ctatattacc gaatatatat ataaaggaga aaaacaccag ggtgagaatg ccttgaccaa
     2521 ggaactattg gaagcggctg atctggtaat agtgacagct gctcacacta aggtggatta
     2581 caatttcgtc caacaacact ctaagttcat ttttgacacc aaaaatgcaa tgaaagctgt
     2641 tgtcaatagg gaaaacatag agttgctgta gcaattctat gttttcagtt ccgagtataa
     2701 agtattgagt tctgagttat gaaaagaatt tgcgttattg gtgggggtaa atggggacag
     2761 aatcatatcc gcacactatt cgaaatggga aatctcggcg gtatcgttga atccaacccc
     2821 aaaagattga aagagctgct ggagaaatat ccggtaaagg gttttcagga tgttgaggca
     2881 gctgttaaag agaattttga cggatttaca atagccgcac cggcaccact tcactatgag
     2941 attgccaaac ttttgataca aaaccgcaaa aacgtattgg tggagaagcc gatgaccctc
     3001 tcttccgaaa catctctcga attggttgag ctggccaaag caaaaggagt tcgactgatg
     3061 gttggccatg tactgctctt tcatcctgca atacgtaaaa taaaagaggt catagatgac
     3121 ggtaaaatcg gggaacttta ctacatctat tccaatagac taaatcttgg tactgtacgg
     3181 acggaggaga atgtcttttg gtcctttgca ccacacgatg tttcggt
//
LOCUS       DAOSIE010000163         3200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_26317_length_3200_cov_3.478219, whole genome shotgun sequence.
ACCESSION   DAOSIE010000163 DAOSIE010000000
VERSION     DAOSIE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26317_length_3200_cov_3.478219"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLI69_05280"
     CDS             complement(<1..382)
                     /locus_tag="PLI69_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOG41932.1"
                     /translation="MNNIVNMIADKIRIEEYDYPLPDERISKFPLQERDSSKLLLFTG
                     RNNFSPAEAKFSDIDRFLPENSLIIFNNTKVIPARLIFKRDSGALIEIFCLEPADPGD
                     YQQNFACTEKCRWKVIVGNIKRWKE"
     gene            975..1574
                     /locus_tag="PLI69_05285"
     CDS             975..1574
                     /locus_tag="PLI69_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG41933.1"
                     /translation="MDKRLQQFLDLEDLTPSKLADILKIQRSGISHLLSGRNKPSFDF
                     IQGILINFPHINPDWLILGKGKPYRSQTATSVTSPTPMSKDTVVPSSSPYTLFDTPVI
                     KTQESLPDSTQFISETKADSQIEVVSVPSALNDVQSETGPTTPVTQHITKISEASQPS
                     EDRFMEPSSTISPNIQASKRDKRIVRITVFYSDGTFEEK"
     gene            1605..2687
                     /locus_tag="PLI69_05290"
     CDS             1605..2687
                     /locus_tag="PLI69_05290"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOG41934.1"
                     /translation="MYKLFIRPVLFLFNPERAHKIIFSLLKFSGHIPGLQALLRLIYR
                     VKTPTLERNLFGLHFKNPVGIAGGLDKNAECYNELACFGFSFIEIGSLTPKAQAGNPK
                     PRSFRLVKDNALINRMGINNLGVRNAIDNLRSNPPRDVIIAANISKNSTTSNEDAAED
                     YEKALTLMYDFADMTVLNISCPNVKDLTELQDIEMLSNIIDKIITIRKFNEEYHPILL
                     KLSPDIPFEQIDEIIDLVQISGIDGIVATNTTRSREGLVTPCEDVKKIGEGGLSGAPI
                     FEKSLRMVKYIHNRTRGSIPIIGVGGIMTPEQAQEMLDAGASLIEVYTGFIYNGPGYV
                     KKILKHLRNVEKERSLAATTNNTPSA"
     gene            2689..>3200
                     /locus_tag="PLI69_05295"
     CDS             2689..>3200
                     /locus_tag="PLI69_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOG41935.1"
                     /translation="MTTASICTIGDEILIGQITNTNSSEISRALNRSGIRVRYHLSIG
                     DLHEDIVSKLSFCLEQTDIVIVTGGLGPTRDDITKLALKELTGASSFVRSEEQYRIIE
                     RLMSARGIPMIESNREQSTVPDTCEVIPNELGTAPCMVFRQVGPKGTSTLYSLPGVPF
                     EMMGLLPKVM"
BASE COUNT         1017 a          680 c          594 g          909 t
ORIGIN      
        1 cttccttcca tcttttaata tttcctacaa tgactttcca gcgacatttc tccgtacaag
       61 caaaattttg ttgataatcc ccgggatcag ccggttccag acaaaaaatc tcaattaaag
      121 cgccggaatc acgcttaaaa atcaatcttg cgggaattac cttagtatta ttgaagatta
      181 tgagggagtt ttcgggcaaa aaacgatcta tatccgagaa ttttgcttca gcaggtgaaa
      241 aattgttcct acctgtaaaa agtagcaact tggaagagtc gcgctcctga agaggaaatt
      301 tagaaattct ctcgtcagga aggggataat catattcttc aattctgatc ttgtccgcaa
      361 tcatatttac aatgttgttc acaaaggtaa aactttagat ttgaataaag gccaatttta
      421 agcgatttaa gaggtttaca tctgtaggtg taaagaatct atttgatgtc aaattaaatt
      481 gctctggtgc aatttgttta catttgtgaa atgacgattt ggttacaaat gtctaccaaa
      541 gtaatcccgt tcatttacat ttgtaaatta tacaagtctg agaggtgggt ttacagatgt
      601 gaggggaata tttataaaag ttatcaacat atgttaataa ctaaaagtta ttttaaatta
      661 tttatgagat taaaatattg aaactcagtt aattatctat aattatctaa aggatattta
      721 tgaatacaac agtaaattat ggctgtaaaa gcccttttcg tagttaaaat ggataaaata
      781 ttagattaat atattaaaac ccaataactt aataatttta tctaaaataa aacttcatat
      841 taaatccaca tttatactcc tatcctgtcg tttatttttg taatttacat ttgtaatcat
      901 taaaatatct tccgtttatt acatttgtaa tcaaaataat cattacattt gtatcaaact
      961 aagtttacaa gattatggat aagcgtttac aacaattttt ggatctggag gacctcactc
     1021 cctcaaaact tgccgatata ttgaagatac agcgctccgg tatttcccat cttctctccg
     1081 gacgcaataa acccagtttt gattttattc aagggatact catcaatttt cctcacataa
     1141 atcccgattg gcttattctt ggaaaaggta aaccttacag gagtcaaact gcaacatctg
     1201 taacttcccc cactccgatg agtaaagata cggttgtacc atcttcttcc ccttatactc
     1261 tttttgatac tccggtcatc aaaactcagg aatcactccc cgattcgact caatttatct
     1321 ctgaaaccaa agctgatagc cagattgaag tagtttccgt gccgagcgca ttaaatgacg
     1381 tacagagtga aacaggcccc actactcctg taactcagca tattacaaag atttctgagg
     1441 cttctcaacc ctctgaggat cgatttatgg aaccctcttc taccatcagt ccgaatattc
     1501 aggcctcaaa aagagataaa aggatagttc gcattaccgt tttttatagc gatggcacat
     1561 ttgaagaaaa atgactactt ttgtaatgac aatcattaca taatatgtac aagcttttca
     1621 taaggccggt tctctttctc ttcaatcctg aaagggcgca taaaataatt ttctcgttac
     1681 ttaaattttc cggacatatt ccgggactcc aggctctcct gcgacttatc tatagagtca
     1741 agaccccgac acttgagcgt aacctcttcg gtcttcattt caagaatccc gtaggaatag
     1801 ccggaggcct tgacaaaaat gcagaatgct acaatgaact ggcctgcttc ggatttagtt
     1861 ttatagagat aggatcgctc acaccaaagg ctcaggcagg aaatccaaaa ccacgcagct
     1921 tccggctcgt taaggacaac gcacttatca atcgcatggg aatcaataac cttggagtga
     1981 gaaacgctat tgataatctg aggagtaacc caccccgcga tgtcataatt gcagccaaca
     2041 tcagcaaaaa ttccaccaca tcaaacgaag atgcagctga agattacgaa aaggccctga
     2101 cattgatgta tgattttgcg gatatgaccg ttctcaatat ctcctgtccc aatgtcaagg
     2161 accttacgga actacaggac atagagatgc tctcaaatat cattgacaag atcattacca
     2221 tccgcaaatt caacgaagaa taccacccca tactcttaaa actctcgcct gacattccat
     2281 ttgagcagat tgatgagata atagaccttg tacagattag cggtatagac ggcattgtag
     2341 caactaatac tacacgcagt cgcgagggac ttgtgactcc ctgtgaagat gtaaagaaga
     2401 tcggtgaagg aggtcttagc ggagccccta ttttcgaaaa atcactaagg atggtaaaat
     2461 atatccacaa taggacccga ggctccatac ctattatagg cgtaggcggc ataatgaccc
     2521 cggaacaggc acaggagatg ctggatgcgg gtgcatctct gatagaagta tatacgggat
     2581 ttatatacaa tggcccggga tatgtaaaga aaatccttaa acacctacgc aacgtagaga
     2641 aagaaaggtc tttggcggcc actaccaata ataccccttc ggcttaaaat gactactgca
     2701 tcaatatgta ccattggcga tgagatacta atcggccaaa taaccaatac caactcctca
     2761 gagatatcaa gagctctcaa ccgatccggt ataagagttc gctatcacct ctcaataggt
     2821 gatctccatg aggatattgt atccaaattg agtttttgcc tggagcagac agatatagtg
     2881 atagttacgg gaggtcttgg tccaaccaga gacgacatta ccaaacttgc cctcaaggag
     2941 ctcacggggg catcatcttt tgtccgctcg gaagaacaat atagaatcat agagagactg
     3001 atgagtgcca gaggcattcc catgatagag tcaaatagag agcagtctac cgtccccgac
     3061 acctgtgaag taatccccaa cgaactgggt accgctccct gcatggtctt tcgccaggta
     3121 ggcccaaaag gcacctctac gctctattcg ctccccggag tcccttttga gatgatggga
     3181 ttgcttccga aagtaatgga
//
LOCUS       DAOSIE010000164         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_26481_length_3184_cov_3.485139, whole genome shotgun sequence.
ACCESSION   DAOSIE010000164 DAOSIE010000000
VERSION     DAOSIE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26481_length_3184_cov_3.485139"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2225
                     /locus_tag="PLI69_05300"
     CDS             <1..2225
                     /locus_tag="PLI69_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG41936.1"
                     /translation="KNNQITFELKRDTTINFYLEEAPDSIDEAVVYGESRFHGVRSSQ
                     LSAVDIPILQIKSTPTLFGEQDVLKTLQLLPGVQAGNDGTAGLYVRGGGQDENLLLLD
                     GVPVYNVNHALGFFSVFNPDAVKSVTLFKGNFPARFGSRLSSVIDVRTNDGDMYDYHG
                     NFSIGLISSKINVEGPIIKGKTSFNLSARRTYLDAILVPAMYFATKITSKGEEKGHAG
                     YNFYDINAKVNHKFSDRDRLFLSYYTGDDALHAKMRANYAYADGATWEEYMGVNWKWG
                     NMLATARWNHVCSPRLFMDASVNYTHYRNKMGVSYEDIGEYPNPSENYSYESLVTANS
                     GINDLTAKVDFSWNPATDHNVRFGAAYIYHKFSPDVSSNRITVKTPIKGVNGQVKDTT
                     EIVEQMYGDKKINASEINAYVEYKISLGKLFEANVGLHYSAFAVGKEFYNSVQPRLSA
                     RILLAEKMSLKLGYAHMSQYVHLLTSNSISLPTDLWVPVTERIKPMSSHQYSAGVFYD
                     MAGYNFSIEGYYKTMDNLLEYIDGASLMATTTGWEDKVAMGRGWSYGAEFLVQRSIGR
                     TTGWIGYTWSKSMRQFDREGQVIDNGRPFPSKYDRRHDISITVSHKLSDRIDFAASWV
                     FNSGNCATLALQHYPQMPEDFIYDASMGYTIKNIGYIPSRNNYRLPAYHRLDLGINFH
                     KQKKHGIRTWNISIYNAYNNMNAFMVYPGYDDKNSEMKKLKKLTIFPIIPSVSYSYKF
                     "
     gene            2247..>3184
                     /locus_tag="PLI69_05305"
     CDS             2247..>3184
                     /locus_tag="PLI69_05305"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOG41937.1"
                     /translation="MNKVIRFLTLILVTVSFSACENNIEFPDRLEEKYMVLYSMAEAG
                     KPVLASVGESVFFLDNVPIQNWRKDATVSLYINGEFIEHLKVYDRNPPHQPVDGDEYY
                     KSDAIVNSGDEVRIEASLPDFNEVTSGSTVVPYPTSVIDVKVISLVPNENGNGYYGKL
                     QITFSDDENSNDYYWVSAKKRAEYYPEHPDWIDNWIDVKYEDKIFAEKVDYIIEAFGE
                     PKRSYSYGFFDDRSINGIEKYPLTLGFYHNPEVSKGPLTFDISLCTIDENYYKYLKSY
                     RQSFDNSVLQEPVQVHSNIKNGIGLVGSKANSISEE"
BASE COUNT          973 a          601 c          679 g          931 t
ORIGIN      
        1 ataagaataa tcagattact tttgagctta aacgagatac gacaataaat ttctatttgg
       61 aagaagctcc ggactctatt gacgaagcag ttgtctatgg tgagtctcgt tttcatgggg
      121 ttcgaagttc acaacttagt gctgtagaca ttcctatatt gcaaattaaa agcaccccta
      181 cactttttgg agagcaggat gtgctgaaaa cacttcagtt gttacccggc gttcaggccg
      241 gtaatgatgg cactgcagga ctatatgtgc gtggtggtgg tcaggatgaa aatttacttt
      301 tactcgatgg tgttcccgtt tataatgtaa atcacgcttt aggattcttc tctgtattta
      361 atcctgatgc cgtcaaaagc gttacacttt tcaaaggaaa ctttccggcc cgctttggct
      421 cacgtctttc atcagttatt gacgtgcgta ccaacgatgg tgatatgtac gattatcacg
      481 gcaacttcag tattggacta atctcttcaa aaatcaatgt tgaaggcccg ataattaaag
      541 gaaaaacctc ttttaatctc tcagcacgcc gtacctattt agatgcaatc ttagttccgg
      601 caatgtattt tgcgacaaaa attacaagca aaggtgaaga gaagggacat gcaggttata
      661 acttctacga tattaatgct aaggttaatc acaagttctc ggaccgggat agactctttc
      721 ttagttacta taccggtgat gatgcacttc atgcaaagat gcgtgccaac tacgcctacg
      781 ccgatggtgc aacttgggag gaatatatgg gtgtaaattg gaaatgggga aatatgcttg
      841 caacggcaag atggaaccat gtatgctctc caagactgtt tatggacgca tcagttaact
      901 acacacatta tcgtaacaag atgggagtgt catacgagga tattggtgaa tatccaaatc
      961 caagcgaaaa ctactcatat gaatctttgg ttacggctaa ttccggtata aacgatctta
     1021 ctgctaaagt ggatttcagt tggaacccgg ctactgatca taatgtgcgt tttggtgctg
     1081 catatattta tcataaattc tctccggatg tctcttcaaa tcgcatcaca gtcaagactc
     1141 cgattaaggg tgtgaatgga caagtaaaag acaccactga gattgttgag cagatgtatg
     1201 gagataagaa gatcaacgca agcgaaatca atgcttacgt agagtataaa atttctcttg
     1261 gtaaactatt cgaagcaaat gtcggattac attactcggc ctttgctgtc ggcaaggagt
     1321 tttacaattc ggtgcagcca cgactgagcg cacgtattct tctggcagag aagatgtcac
     1381 taaagttggg ctatgcacat atgtcgcaat atgttcacct tttgacaagc aactcaatca
     1441 gtctcccgac agacctttgg gtacccgtga ccgagcgcat caaaccgatg agctcacatc
     1501 aatattctgc cggagtattt tatgatatgg caggatacaa cttctctatt gagggttact
     1561 ataagaccat ggataatctg ttggaatata tcgacggcgc ttcgctgatg gctaccacta
     1621 caggatggga agataaagtg gcaatggggc gtggctggtc atacggtgca gaattccttg
     1681 tgcaacgttc aataggcaga actaccggtt ggataggtta tacctggtct aaatcaatgc
     1741 gtcaatttga tagggaaggc caggttattg ataatgggcg gcctttccct tcgaaatacg
     1801 accgccgtca cgacatcagc atcactgtct cacacaaact ctctgaccgc atagacttcg
     1861 cagcttcgtg ggtctttaat agtggtaact gtgcaacact cgctctgcaa cactatcctc
     1921 agatgcctga agattttatt tatgacgcct caatgggata tacaatcaaa aatattggat
     1981 atattccatc ccgcaacaat taccggttac cggcatatca tcgccttgat cttggaataa
     2041 atttccataa acagaaaaaa cacggtataa gaacctggaa tataagtatt tataatgcgt
     2101 acaacaatat gaacgccttt atggtatacc ccggatatga tgataagaat tcggaaatga
     2161 agaaacttaa gaaactgacc atatttccca ttattccgtc agtaagttac tcgtataaat
     2221 tttaaacact cttcagataa ttaattatga ataaagttat aagattttta acgttgattc
     2281 tggttacagt atcatttagt gcgtgtgaaa acaatataga gtttcctgat cgccttgaag
     2341 agaaatatat ggttttatac tcgatggctg aagccggcaa accggttttg gcaagtgtag
     2401 gtgaaagtgt cttcttcctt gataatgttc caattcaaaa ttggagaaag gatgccacag
     2461 taagtctata tatcaatgga gagtttatag agcatttgaa agtatatgac agaaatccac
     2521 cgcatcaacc tgttgatgga gatgagtatt acaagtccga tgcaattgta aatagtggtg
     2581 atgaagtgcg catagaggca tccttgcccg atttcaatga ggtgacaagt gggtcaacgg
     2641 tagtccctta tccgacatcg gtaatcgatg taaaagtaat ttcattagta ccgaacgaaa
     2701 atggcaatgg atattatggc aaactgcaga taactttcag tgatgatgaa aatagtaatg
     2761 attattattg ggtaagtgct aaaaagcgtg cagaatatta tcctgaacat cctgattgga
     2821 ttgacaattg gatagatgtc aagtatgagg ataaaatatt cgcagaaaag gttgattata
     2881 tcatagaggc ttttggtgaa ccgaagagat cttactctta tggttttttc gatgataggt
     2941 caataaatgg tattgagaaa tacccgttga cattaggttt ttaccataat cctgaagtca
     3001 gcaaaggtcc tctcaccttt gatatttccc tttgtacaat tgatgagaac tactacaagt
     3061 atcttaagtc ataccgtcaa tcctttgata actctgtttt gcaagagcct gtccaagtcc
     3121 actccaacat caaaaatggt attggcttag taggatctaa agctaattct atatccgaag
     3181 agtt
//
LOCUS       DAOSIE010000165         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_26537_length_3177_cov_4.260730, whole genome shotgun sequence.
ACCESSION   DAOSIE010000165 DAOSIE010000000
VERSION     DAOSIE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26537_length_3177_cov_4.260730"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="PLI69_05310"
     CDS             complement(<1..142)
                     /locus_tag="PLI69_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HOG41938.1"
                     /translation="MRRVVILLGPTAVGKTDYSIELALHFGSHIISCDSRQLFREMSI
                     GTA"
     gene            complement(139..618)
                     /locus_tag="PLI69_05315"
     CDS             complement(139..618)
                     /locus_tag="PLI69_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41939.1"
                     /translation="MIIYRYRATIPKSKVFYRIYEVKSNMTLFAFNSFILADLEFAPD
                     QVVMFEGCDAEGYLSSEYGMFDTGDGTMDCVTFEDTLKKGEPILNYIYDISDDRYIRL
                     EFIDEVKFLPMRSYPHNSEGKGHNPDQFSSRYEDIVEYREEILEKPEELLDTEDLSE"
     gene            676..1290
                     /locus_tag="PLI69_05320"
     CDS             676..1290
                     /locus_tag="PLI69_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOG41940.1"
                     /translation="MNSSSKVVNDCYLSIAEPSQGLYKEKGSKFLAFAYPVEQEEQAM
                     EIVADLKKEYFDARHHCYAYRIGLTGDKWRMNDDGEPSSSAGRPIYGQLLSNELSDIL
                     VVVVRYFGGIKLGVPGLIRAYKSATADAIANAEIIEKIAVEFFTITFDYLQMNDVMKV
                     LKEMNMTTISQSFDLTCTMEVRVRLTQIEQFYESLKNTQICRKT"
     assembly_gap    1338..1347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1348..2171
                     /gene="pyrB"
                     /locus_tag="PLI69_05325"
     CDS             <1348..2171
                     /gene="pyrB"
                     /locus_tag="PLI69_05325"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOG41941.1"
                     /translation="NKSQNILNDKVVACIFFEPSTRTRLSFETAANRLGARVIGFADA
                     AHTSIAKGESLKDTIKMVSNYVDIIVIRHPLEGAARYASEVTPVPVINAGDGANQHPT
                     QTLLDIYSINKTQGRLDDLTINMVGDLKYGRTVHSLLQAMSYFSPHFFFTSPDELKIP
                     LEYKRFLDRKGISYTETKQLSDHLDDTDILYMTRVQQERFNDPIEYENVKNVFRLEAS
                     MLSNVRNNLKILHPLPRINEIAADVDDTPYAYYFKQAENGMYVRMAIIAYLLGYR"
     gene            2182..2655
                     /gene="pyrI"
                     /locus_tag="PLI69_05330"
     CDS             2182..2655
                     /gene="pyrI"
                     /locus_tag="PLI69_05330"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOG41942.1"
                     /translation="MEQKDKHLKVTAINNGTVLDHIPAESLFKVIDILNLSKRPNQIT
                     IGSNLDSRSMGKKGIIKISEHFFEDEEIGEIALIAPNATINIIRDYEVVEKKIISIPE
                     SFIGIFKCMNPMCVTNHQDIPTKFTTIRERNEVLLLCHYCEKTTDTRNLKIVSKK"
     gene            2716..>3177
                     /locus_tag="PLI69_05335"
     CDS             2716..>3177
                     /locus_tag="PLI69_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG41943.1"
                     /translation="MKKVHNFNAGPCVLPKQAIDAAIAALQDFKGTGMPVISVSHRSK
                     EWESVMDECRALWKELLDIPDDYEVLFLGGGASLQFLYVAMNLLENKAGYLETGVWAK
                     KAFKEAKSIGNAVCIASSADKTYSYIPKGYEIPTDLDYFHITTNNTIYGTEI"
BASE COUNT          990 a          741 c          631 g          805 t
ORIGIN      
        1 cagcggtacc aatactcatc tctctgaata gttggcgaga atcacaactg attatgtgag
       61 acccgaaatg aagtgccagc tctatgctgt aatctgtctt ccccaccgcc gtagggccga
      121 gcaatataac gactcgtctc attcggagag atcttctgta tccaataact cttccggctt
      181 ttctaaaatt tcctccctgt attccacgat atcttcatag cgtgatgaaa attgatccgg
      241 attatggccc ttaccctccg agttgtgggg atatgagcgc atcggtagaa acttaacctc
      301 gtcaatgaac tccagacgaa tatatctgtc atcagagatg tcgtaaatat agtttaaaat
      361 aggctcaccc ttttttagag tatcttcgaa tgttacacaa tccatggtgc cgtcacccgt
      421 atcaaacatt ccatactcgc tgctcagata accttcggca tcacatccct caaacattac
      481 aacctggtcg ggagcaaatt ccaaatctgc aagaataaag ctgttaaaag caaaaagagt
      541 catattggac tttacctcat atatcctgta gaaaactttg ctcttcggta tggtagccct
      601 atatctgtaa ataatcattg gcgatgagtt tagtattgca aaattactaa aaaagctata
      661 tttttacaat tcggaatgaa ttcatcatca aaagttgtta atgactgcta ccttagcatc
      721 gcagaaccct cacagggcct ctataaggag aagggtagca agtttctcgc ttttgcatat
      781 cccgtggaac aagaggagca ggcaatggag attgtagccg atctcaaaaa agaatatttc
      841 gacgcaagac accattgtta cgcttaccgt atcggactta ccggagataa atggcgcatg
      901 aatgatgatg gagaaccctc ttcgtcagcc ggcagaccca tatatgggca acttctttcc
      961 aatgaattga gtgatatcct ggtggttgtg gtaaggtatt tcggaggtat caaactcggt
     1021 gttcccggcc tcataagagc ttataagagt gcaaccgctg acgcaattgc caatgctgag
     1081 attatagaga aaattgccgt agagtttttc accataacct ttgattatct ccagatgaac
     1141 gatgttatga aagtgctcaa agagatgaat atgaccacca tttcacagag ttttgacttg
     1201 acctgtacta tggaagtaag agtgcgtctg acacagattg aacagtttta cgaatcactg
     1261 aaaaacacac agatatgccg aaaaacctaa tttcaattca cgatttctca aaagaggaga
     1321 ttctccacat tgtggagnnn nnnnnnnaga ataaatcgca gaatatcctc aatgacaagg
     1381 tggttgcctg catttttttc gagccgtcca ccaggactcg tctcagtttt gagactgcgg
     1441 ccaaccgcct gggagcccgc gtaataggct ttgctgatgc agcccatacc agtattgcca
     1501 aaggagaatc ccttaaggac accatcaaga tggtatccaa ctatgttgac atcatcgtga
     1561 taaggcatcc gctcgaagga gctgccagat atgcctccga agtcacaccc gttcccgtta
     1621 tcaacgcagg tgacggtgcc aaccagcatc ccacccagac tctgctcgac atctatagca
     1681 tcaacaagac tcagggacgc ctcgacgacc ttacaatcaa tatggtgggt gacctcaaat
     1741 atggacgcac cgtacactcc ttgcttcagg caatgtccta cttctcaccc cacttcttct
     1801 tcacctcacc ggatgagcta aagatacccc tcgaatacaa aagatttctt gatagaaaag
     1861 gaatatccta tacagaaacc aaacaactct cagatcacct tgatgacacg gatatcctct
     1921 atatgaccag agtgcagcag gagcgcttca acgacccgat agagtatgag aatgtaaaaa
     1981 atgtcttccg tctggaggca tccatgctct caaacgttcg caacaacctt aaaatccttc
     2041 accctctccc cagaatcaat gagattgccg ccgatgtgga tgacactcct tatgcatact
     2101 atttcaaaca ggccgaaaac ggaatgtacg tgagaatggc catcatagca tatttattgg
     2161 gatacagata aacagagaat tatggaacag aaagacaaac atttgaaggt cacagccatc
     2221 aataatggca ccgtactaga ccacatacca gccgaaagcc tcttcaaggt aatagacatc
     2281 ctcaacctct caaagcgtcc caaccagatt accatcggca gcaatctgga tagtcgcagt
     2341 atgggtaaaa agggtattat caaaatttca gagcattttt tcgaggatga ggagataggc
     2401 gaaatcgcct tgattgcacc aaatgccacc ataaatataa ttcgcgacta tgaagtagta
     2461 gaaaagaaaa taatatcaat tcctgaaagt ttcatcggca tattcaaatg catgaatccc
     2521 atgtgtgtaa cgaaccacca ggatatccca acaaaattta caacaataag agaaagaaac
     2581 gaggttttgc ttctctgcca ctattgtgaa aagaccaccg atacccgaaa cttaaaaatc
     2641 gtaagtaaaa agtgatttat tgcgaaatta ttatatcttt gtaggatata taacaaataa
     2701 aatataatca aacatatgaa aaaagttcac aatttcaacg caggcccttg cgtattgcct
     2761 aagcaggcca tcgatgctgc aattgctgca ttacaagatt ttaagggtac aggaatgcct
     2821 gttatttcag tttcacaccg ctcaaaggag tgggaatctg tcatggatga gtgccgtgca
     2881 ctctggaaag aactcctcga cattcccgac gactatgaag tactcttcct tggcggcggt
     2941 gcatctctac agttcctcta cgttgcaatg aacctcctcg agaataaagc aggctatctc
     3001 gagacgggtg tatgggcaaa gaaggctttc aaagaggcta agagcatcgg aaatgccgtt
     3061 tgcatcgcct cttcggctga caagacatat agctatattc ccaagggata cgagatacct
     3121 accgacctcg attatttcca tatcaccacc aacaacacca tctacggtac ggagata
//
LOCUS       DAOSIE010000166         3175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_26561_length_3175_cov_4.176923, whole genome shotgun sequence.
ACCESSION   DAOSIE010000166 DAOSIE010000000
VERSION     DAOSIE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26561_length_3175_cov_4.176923"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLI69_05340"
     CDS             complement(<1..661)
                     /locus_tag="PLI69_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOG41944.1"
                     /translation="MRQKRRDFEMWQERTRLLLDPEQMERLRDAHIAVIGLGGVGAYA
                     AEMLARAGVGSLLILDSVNVSLSNRNRQLLALESTTGRPKTEVMVERLRDINPDISLT
                     IINDYLTSENVEQLLSGQRIDFLVDAIDTLSPKIALICYCMSQKIPLVSSMGAGAKFD
                     MTRVRIADISKSFNCPFAYIIRKKLRKEGISKGFKVVFSEELPDREAIIPVEETNKKS
                     QV"
     gene            complement(636..1436)
                     /gene="mazG"
                     /locus_tag="PLI69_05345"
     CDS             complement(636..1436)
                     /gene="mazG"
                     /locus_tag="PLI69_05345"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOG41945.1"
                     /translation="MEKDIRLQAFERLLDIMDELRAKCPWDREQTVESLRPLTIEETY
                     ELSEAILAGDSRNMAKELGDILLHIVFYAKIGSEEGTFDITDVINMLNEKLVYRHPHV
                     FSATSVEDSAQVIRNWESLKQTEKGGNKTVLSGVPESLPSLLKAYRMQDKARAVGFDW
                     EERSQVWDKVAEEIGEFRTELDSMEASDPESVARAEGELGDLLFSIVNAARLYDLNPD
                     TALEMTCRRFKERFTYLELKSKELGRNLKDMTLDEMEEIWNEAKKKGL"
     gene            1556..2857
                     /locus_tag="PLI69_05350"
     CDS             1556..2857
                     /locus_tag="PLI69_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOG41946.1"
                     /translation="MRNIKFISADEAVKVVKSGDHVHLSSVASAPQILIQALCRRGDA
                     GELRDVRIHHLHTEGEAPYADPKYEGIFFHQAFFVGSNVRKSVQAGYADYIPILLGET
                     QKLYRGGYLPCNVAMIQVSPVDEHGYVSLGTSVDATLAAIECAEHVIAVVNENVPRSW
                     GDAMIPLSMIDLFVEDNTPLEPAHFTEPTEIEKAIGMHCANLIEDGATLQMGIGAIPN
                     AVLAQLGNHKNLGIHTEMFADGVLPLVEKGVINGANKTLDKGKMVATFLMGSQTCYDF
                     IDDNPMVLMMDVGYTNDPFVIAQNPMVTAINSALQVDITGQVCADSLGTKFYSGVGGQ
                     MDFTYGASRSRGGKAIIAMPSATNKGVSKISPILTPGAGVVTTRNHIHWFVTEYGAVN
                     LYGKSLQERAKLIISIAHPDAREELDKTAFERFGPHFHFVK"
     gene            2943..>3175
                     /locus_tag="PLI69_05355"
     CDS             2943..>3175
                     /locus_tag="PLI69_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG41947.1"
                     /translation="MTKVQKTTTRYSDCFKLQVVEEIENNGLSIKDCRLKYGIGGGST
                     IQKWLLKYGKQHLLNKIVRVETRDEIDELKRLRA"
BASE COUNT          849 a          788 c          742 g          796 t
ORIGIN      
        1 ccacctggga tttcttattg gtttcctcca ccgggataat ggcttcgcgg tcgggcagtt
       61 cctccgaaaa gactacttta aaaccctttg aaatgccctc cttgcgaagt tttttacgga
      121 taatgtaggc aaaagggcag ttaaaggatt ttgaaatatc ggcaatcctg accctggtca
      181 tatcaaattt ggctccggct cccatggagg agacgagagg tattttctgc gacatgcagt
      241 agcaaatgag tgctatcttt ggggagaggg tatcaatggc atccacgaga aagtctatcc
      301 gttgtccgga gagaagctgc tctacatttt ccgaagtaag ataatcattg attatagtaa
      361 gagaaatatc ggggttgata tcccgcaggc gctcaaccat cacttcagtc ttgggtctgc
      421 ctgtagtaga ctccagcgca agcaactgac ggttgcggtt ggaaagggaa acattgacgg
      481 aatcgagtat cagcagcgat cccacaccgg cacgggcaag catttcggcg gcataagcgc
      541 ctactcctcc aagccctatg accgcaatgt gggcgtcgcg cagcctctcc atttgctccg
      601 gatcgaggag caatcttgtc ctctcctgcc acatctcaaa gtcccttctt ttttgcctca
      661 ttccagatct cttccatctc gtcgagggtc atatccttta aattgcgtcc aagctctttg
      721 ctcttaagct caaggtaggt aaatctctcc ttgaacctac ggcaggtcat ctccagagca
      781 gtatccggat tgaggtcgta tagacgcgcc gcatttacaa ttgaaaatag cagatcaccc
      841 aactcgcctt cagcccttgc aaccgactcc ggatcggaag cctccataga atcgagctcc
      901 gtgcgaaact cccctatctc ttcggccacc ttatcccata cctgcgatcg ctcctcccag
      961 tcaaatccca ctgcccttgc cttgtcctgc atacgatagg cctttagcag cgaaggtaag
     1021 gattccggga ctccggaaag caccgtcttg ttaccaccct tttccgtctg tttcagggac
     1081 tcccagttgc gtatcacttg agcggaatcc tccacggaag tggctgaaaa gacatgcggg
     1141 tgacgataga caagtttttc gttgagcatg tttatcacat cggtgatatc gaaggtaccc
     1201 tcttcactgc ctattttggc gtaaaaaaca atatggagca aaatatctcc caactccttt
     1261 gccatattgc gcgaatcacc cgcaaggatg gcttcactca gttcgtaagt ctcttctatg
     1321 gtgagcggtc taagggattc aactgtctgt tccctatccc agggacattt ggcacgaagc
     1381 tcatccatga tgtcgagcaa ccgctcaaat gcctgcaatc tgatatcttt ctccattggc
     1441 ttttttgtta aacatcggca aaattaccaa aagtttccca aatcggatat gttttaatac
     1501 atttgcaccc tacattaatc attaactgaa agtaacggga aaaaacagta ctgaaatgcg
     1561 aaatatcaag tttatcagcg ccgatgaggc agtaaaagtg gtaaaatcgg gtgaccacgt
     1621 ccatttgagc agcgttgcaa gcgctcccca aatcctgata caggcactct gtcgccgcgg
     1681 tgatgcgggc gaactcaggg atgtacgcat ccatcacctc cataccgagg gtgaagcccc
     1741 ctacgctgac cccaaatacg aaggtatctt cttccatcag gcatttttcg taggaagcaa
     1801 tgtacgcaag agcgttcagg ccggctacgc cgattatatt cccattttac tgggcgagac
     1861 acagaagctc tacaggggcg gctatctccc ctgcaacgtg gcaatgatac aggtatctcc
     1921 ggttgatgag cacggatacg tctccctcgg cacctcagtt gatgctactt tggctgcaat
     1981 agagtgcgcc gaacacgtca tagccgtagt caacgaaaat gtaccccgct cctggggaga
     2041 tgcgatgata ccactatcca tgatagattt gtttgtggaa gacaatactc cactggagcc
     2101 ggctcacttc accgagccaa cggagataga gaaggcaatc ggtatgcact gtgccaacct
     2161 catagaggat ggtgcaactc ttcagatggg aatcggggca attccaaatg ccgtgttggc
     2221 tcagcttggc aatcataaaa acctcggcat ccacacggag atgtttgcag atggtgtgct
     2281 ccccctggtg gaaaagggtg tgatcaatgg agccaacaaa accctggaca aaggtaaaat
     2341 ggtagccacc ttcctgatgg gttcgcagac ctgctacgac ttcatagatg acaatccgat
     2401 ggtattgatg atggatgtcg gttacacaaa tgaccccttt gtcattgcac agaatcctat
     2461 ggttaccgct atcaactcgg cattacaggt ggatatcaca ggtcaggtgt gtgctgactc
     2521 tctgggcacc aaattctact ccggcgtagg tggacagatg gactttacct acggtgcttc
     2581 cagatccagg ggcggcaagg caataattgc aatgccttca gctaccaata aaggtgtgag
     2641 caagatctcc ccgatcctga ctcccggtgc aggtgttgtt actacacgca accacataca
     2701 ttggtttgtc acggagtatg gtgcagtaaa tctctacggg aagtccctcc aggagcgggc
     2761 aaagcttatc atcagcatcg ctcatcccga tgcgagagag gagcttgaca aaactgcatt
     2821 tgagcgtttc ggaccacatt ttcacttcgt aaaatagagg tgagttcgga gttcggagtt
     2881 ccgagttatg agttcggagt gtcatgtcct gaaataggtt tacacttaaa tcaggaaaaa
     2941 ttatgacaaa agttcagaaa acaacaacac ggtatagcga ttgctttaaa ttacaagtag
     3001 tagaagaaat tgaaaataat ggtttaagca ttaaagattg tcgtctaaaa tatggcattg
     3061 gcggcggatc taccattcaa aaatggcttc taaaatatgg taaacaacac ctattaaata
     3121 aaatagttcg cgtggaaaca agagatgaaa tagacgaatt gaaacgcctt cgagc
//
LOCUS       DAOSIE010000167         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_27051_length_3129_cov_3.372479, whole genome shotgun sequence.
ACCESSION   DAOSIE010000167 DAOSIE010000000
VERSION     DAOSIE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27051_length_3129_cov_3.372479"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PLI69_05360"
     CDS             <1..568
                     /locus_tag="PLI69_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG41948.1"
                     /translation="GIRLTVARFYTPTGRSIQKPYSDKYDMDIIERYEHGEMMSADSI
                     KVNDSLKYVTPGGRVVYGGGGIIPDVFVPLDTLGISDYLIKCNRQSLVIKFANQFSDK
                     HRARMRGIKDMDALYAFFAERDLKKEFIEYTTANGVIPSKKDWEISGEIMMTQVKAVI
                     GRYTPMDDDAFYPIYLKNDNVVKRALGE"
     gene            complement(598..1332)
                     /locus_tag="PLI69_05365"
     CDS             complement(598..1332)
                     /locus_tag="PLI69_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOG41949.1"
                     /translation="MSDEFHKNKTGKRVGLYLTLIFHLSVLIVLLLVSINSLVAPEAS
                     FVLDFTKQEQIEQEQKAIELKEDISRELDQLLATPRAQIRNVVVDIGSQLRDDRHSNP
                     NRIYEEARELQDKLNASKEDALKQESSETVDLTPEPENEQKKKEAAPTYKGPSVISYR
                     LEGRKAQHLPVPAYKGYGSGDVFVEIAVNPRGRVIDARVLETISSPDKSLHDFAVEAA
                     KRSSFTASESAGSRQTGEIVYRFIGQ"
     gene            complement(1332..1949)
                     /gene="udk"
                     /locus_tag="PLI69_05370"
     CDS             complement(1332..1949)
                     /gene="udk"
                     /locus_tag="PLI69_05370"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOG41950.1"
                     /translation="MLIIGIAGGTGSGKTTVVKKISERLHNKVVVIPQDSYYKDQSHL
                     PIEERQELNFDHPDAIDFELLVQQLKELKEGKIIDQPVYSYITCSRSATETVKVGPAP
                     VIIVEGILIFTCKGLRKLMDIMVYVDADDDDRLIRVISRDIMERGKSVEKVMERYQKT
                     VKPMHLQFIAPSKRYADIIVPQGGHNKVAINILIATIGQALENNN"
     gene            complement(1968..>3129)
                     /gene="asnS"
                     /locus_tag="PLI69_05375"
     CDS             complement(1968..>3129)
                     /gene="asnS"
                     /locus_tag="PLI69_05375"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOG41951.1"
                     /translation="LVASLGSGQSVEIQAREIEVLGTADPDIYPLQKKGHSMEFLREI
                     AHLRPRTNTFGAILRIRHAMAFAIHKFFNDKGFVYLHTPIITSSDCEGAGEMFHVTTL
                     NLEDVPKNEDGSVNYDEDFFGRQTSLTVSGQLEGELGAMALGEIYTFGPTFRAENSNT
                     PRHLAEFWMIEPEMAFYEIEDNMDLAEEFIKYLVQYALDNCREDLNFLNNMIDKELIS
                     RLESVVNTKFVRLSYTEGVEILKNSGVKFEFPVAWGIDLQSEHERYLVEKHFKKPVIL
                     TNYPKEIKAFYMKLNEDGKTVRAMDVLFPKIGEIIGGSQREESLEKLEKRIAELDMDH
                     SNLWWYIDTRRFGTAPHSGFGLGFERLLLFVTGMANIRDVIPFPRTPKNAEF"
BASE COUNT          796 a          687 c          718 g          928 t
ORIGIN      
        1 cggtatccgc ctgacagtgg ctcgattcta tacgcctacg ggaaggtcta ttcagaaacc
       61 ctactccgac aaatatgata tggatatcat cgagcgatat gagcatggag aaatgatgag
      121 tgcggatagc atcaaggtga atgattctct caaatatgtc acacccggag gtagggtagt
      181 ctatggtgga ggaggaataa ttccggatgt ttttgtgcct ctggacacac taggaatctc
      241 cgattacctg ataaagtgca atcgacagag tttggttatt aagtttgcta accaatttag
      301 cgataagcat cgtgcacgta tgagaggcat caaagatatg gatgcccttt atgccttttt
      361 tgctgagcgt gatctaaaga aagaattcat cgaatataca acggctaatg gtgtcatccc
      421 ttccaagaaa gattgggaga tttccggaga aataatgatg acacaggtaa aagcggttat
      481 cggtagatat actccgatgg atgatgatgc attctatcct atctatctga agaatgataa
      541 tgttgttaaa agggctctgg gagagtagtt tagagtgtgt agtatgtaga gtgcggttta
      601 ttgtccgata aatcgataaa cgatttcgcc ggtttggcgg gatccggcac tttcggaggc
      661 agtgaagctg gaacgcttgg cggcctctac ggcaaagtcg tgaagtgatt tgtccgggct
      721 ggaaatagtt tccaatacgc gtgcatcaat aacccgtcct ctcggattga ctgcgatttc
      781 aacaaagaca tctcccgatc catagccttt gtaggctgga acaggcagat gctgtgcctt
      841 acgtccttca agacggtagg agatgacgga aggaccttta taggtgggag ctgcctcttt
      901 tttcttctgt tcattttccg gttcaggggt gaggtcgaca gtttcagatg actcctgctt
      961 caatgcatct tccttggatg cattcaattt gtcctgtaat tcgcgtgcct cttcataaat
     1021 ccgatttggg ttggaatggc ggtcatccct gagttgagat cctatatcca ctaccacatt
     1081 ccgtatttgc gctctgggag tggcaaggag ttggtccaat tctcgactga tatcttcctt
     1141 taactctatg gctttctgtt cctgctcaat ctgttcttgt ttggtaaaat caagcacaaa
     1201 tgaagcttcg ggagctacaa gtgaattgat cgatacaagc aacagcacta taagcaccga
     1261 aaggtggaaa ataagggtca ggtacaaccc gaccctcttc ccggttttat ttttatggaa
     1321 ttcgtcgctc attagttgtt gttttcgagc gcctgcccga ttgtcgcaat tagtatgtta
     1381 attgccacct tgttatggcc accctgaggt acgattatat cggcgtagcg tttactgggg
     1441 gctataaatt gcaggtgcat aggcttaacc gttttttgat agcgctccat taccttttcc
     1501 acacttttac ctctctccat gatatccctg gaaataacac gtatcagacg gtcgtcatca
     1561 tctgcatcaa catagaccat aatgtccatc agcttccgta gtcccttaca ggtgaatata
     1621 aggatacctt ctactatgat tacgggagca ggacctactt ttaccgtctc tgtagctgat
     1681 ctggagcagg ttatgtagga gtagactggt tgatctatta tttttccctc cttgagctct
     1741 ttgagctgct gtacaagcag ttcaaagtcg atagcatccg gatggtcaaa attcagctcc
     1801 tggcgctcct caatcggcag atgactttga tctttgtaat aggagtcttg gggaattaca
     1861 actaccttgt tgtgaagtcg ctccgagatt ttcttaacta ctgtggtctt gcctgagccg
     1921 gtgccaccgg ctataccgat gatgagcatg atgatgatat tattgtgtta aaattccgcg
     1981 ttctttggag ttctggggaa tggaatgaca tccctgatat tggccatacc ggttacaaag
     2041 agcagaagcc tttcaaatcc aagtccaaat ccgctatgag gagctgtgcc aaacctgcgg
     2101 gtatcgatat accaccatag attggaatga tccatatcga gttcagctat tctcttttcc
     2161 aatttttcca gagattcttc cctttgagag cctccaataa tttcacctat cttggggaat
     2221 agcacatcca ttgccctaac tgtctttcca tcctcgttga gcttcatata aaaagctttt
     2281 atctccttgg gatagtttgt taggataacc ggtttcttaa agtgtttctc cacaagatac
     2341 ctctcatgct cactctggag gtcaatgccc caggctacgg gaaattcaaa tttcacccct
     2401 gaatttttca gaatttctac accctcggta taggagaggc gtacgaattt tgtgttcaca
     2461 acactttcaa gacgggaaat aagctcctta tcgatcatat tgttgaggaa gttgaggtcc
     2521 tctctgcaat tgtcaagagc atactgtaca agatacttga tgaactcttc agcaagatcc
     2581 atattatctt ctatctcgta gaatgccatt tccggctcaa tcatccagaa ctcggccagg
     2641 tggcggggag tattggagtt ctcagcacga aaagtgggac caaaggtata aatttctccc
     2701 agggccatag ctccaagttc tccttcgagt tgcccgctta cagtcagaga tgtctgtcta
     2761 ccaaaaaagt cctcatcata gttaacactg ccatcttcat ttttgggaac atcttccaga
     2821 ttcagagttg taacatggaa catttcgcct gcgccctcgc agtcggaact tgttataatc
     2881 ggggtgtgaa gatagacaaa ccctttgtca ttgaagaatt tgtgaatggc aaatgccatt
     2941 gcgtgacgga ttctcaatat ggcgccgaaa gtattggtac ggggacgaag gtgagctatc
     3001 tcacgtagga attccatgct gtgccccttc ttttgaagtg ggtaaatatc ggggtctgca
     3061 gtgcctaata cctcaatctc acgtgcctgg atttcgacag attgtccgct acccaatgat
     3121 gccacaagg
//
LOCUS       DAOSIE010000168         3123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_27113_length_3123_cov_3.978162, whole genome shotgun sequence.
ACCESSION   DAOSIE010000168 DAOSIE010000000
VERSION     DAOSIE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27113_length_3123_cov_3.978162"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PLI69_05380"
     CDS             <1..923
                     /locus_tag="PLI69_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG41952.1"
                     /translation="KLIYNLAWKFANNFDFYNKKKFYLAKRNLYDKLVYSKWRAALGG
                     QEMIVVSGGSSIQAKIVRTFNAANLHIYEGYGMTETSPVIAVNSPADGINVIGTVGKA
                     LGGTELSFAEDGEILTRGPHVMLGYYKDPEATAQIIDSEGWLHTGDIGYLVDGQYLKI
                     TDRKKEIFKLSAGKYVAPQVIETRLKESPYIENCIVFGENQKFASAIIIPSVPKLKEY
                     AQKRKIEYNNDQELIATEEIVKLIHSEVEKVNKTLAAHEAIKREKIVLDDWSAENDLL
                     SQTLKLKRRNIHKKYANLINEIFKSDKLED"
     gene            941..1615
                     /locus_tag="PLI69_05385"
     CDS             941..1615
                     /locus_tag="PLI69_05385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG41953.1"
                     /translation="MIRSATKQDISDIRHIYKKSFDASEEYVDFFLKNCNPRHTLVYE
                     QDGKAVSVVQTMSISYISSSRTRGHRDSHPGVYICGLATLPSYRGRGYAKSLVAYIRQ
                     NAKQQGWKFMIVHPSNAEIVSFYDKIGFNTHIYRNSESLPLFENRAFISHFDGIGLFL
                     TRDERLMSDYFQWNHYLLDYIIEESIFNPPSPIQNDTRYPYLLLSSLNDSFATESTHS
                     TFSFPL"
     gene            complement(1823..>3123)
                     /locus_tag="PLI69_05390"
     CDS             complement(1823..>3123)
                     /locus_tag="PLI69_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG41954.1"
                     /translation="FKTSIQRGKEQKPCYIVHTNKNVHEILRTGFEQSPLFSGKIKGI
                     GPRYCPSIEDKLRTFAGKDSHQLFLEPEGWNTHEYYLQGFASSLPLSVQIDALQEIDG
                     LRGVKVFTPGYAVEYDFFDPTELHPTLESRIVPNLYFAGQVNGTTGYEEAAAQGLMAG
                     INAHLKLNNKEPFILKRDEAYIGVLIDDLITKGVDEPYRMFTSRAEYRILLRQDNADS
                     RLTEKGYQLGLVDQFRYQFTRLKYDLISNYIDFANSTTLSPEDVNDYLSSIDSALLSH
                     KKKISDLLSRPSTSISTLLKYVPRGTIIDSNLSEKLSKISSIETEQTRNLLEMPWGKT
                     EGRGDINPEIVSEEILKSAEISIKYKGYIDREKNIAEKIRRLENLRIPDDFDFSKVTS
                     LSMECRIKLNRYRPRTIAQASRISGVSPADIQVLLVYFGR"
BASE COUNT          969 a          642 c          625 g          887 t
ORIGIN      
        1 agaagcttat atataacctc gcttggaaat ttgccaacaa ttttgacttc tacaacaaaa
       61 agaagttcta ccttgccaaa cgcaatctct acgacaaact tgtttacagc aagtggagag
      121 cagctctcgg aggtcaggaa atgattgtag tttcaggcgg gtcttcaatc caggccaaga
      181 ttgtgcgtac tttcaacgcg gcaaacctcc atatatatga aggatatggt atgacggaaa
      241 cctcccctgt aatagccgta aacagcccgg ctgacggcat aaatgtaatt ggcacggtag
      301 gaaaggcact cggaggtacg gaactctcat ttgccgaaga tggggagatt ctgaccagag
      361 gacctcatgt gatgctcgga tactacaagg atccggaagc tacagctcaa ataatagatt
      421 cagaaggctg gttacacacg ggcgatatag gctatcttgt tgacggacag tatcttaaga
      481 ttacagacag gaaaaaggag atattcaaac tttcagcggg caaatatgta gctcctcaag
      541 taattgagac acgcctcaag gaatctccat atatagagaa ctgtatagta ttcggagaaa
      601 accaaaaatt tgcctctgca atcattattc cctctgttcc aaaacttaaa gagtatgctc
      661 agaagcgcaa aattgaatac aacaatgatc aggaactcat cgctaccgaa gagattgtaa
      721 aacttattca ctctgaagtg gagaaagtaa acaagacttt ggcggcacat gaggccataa
      781 aacgtgaaaa gattgtgctg gatgactgga gtgccgaaaa tgatctgctc tctcagactc
      841 tcaaactcaa acgtcgtaac atccacaaaa aatacgcaaa tctcatcaac gaaatattta
      901 agagtgacaa attagaggat tagaatagcc aaacttagat atgattcgct cagctacaaa
      961 acaggacata tcggatatac gccacatcta caagaagagc tttgatgcaa gcgaggagta
     1021 tgtcgatttc ttcctaaaaa attgcaaccc taggcacaca cttgtttatg aacaggatgg
     1081 aaaggccgtt tctgtggtgc aaactatgag catatcatat atatcgagtt cgcgcaccag
     1141 ggggcatagg gattctcatc cgggagtata catttgcggc ctggcaacat taccctcata
     1201 tcgtggtcga ggttatgcca aatcacttgt agcatacatt agacaaaatg ccaaacaaca
     1261 gggctggaaa tttatgattg tccacccttc taatgcagaa attgtctcat tctatgacaa
     1321 aattgggttt aatacacata tctaccgaaa ttccgagtca ctccctctat ttgaaaaccg
     1381 ggcatttatt tcccattttg atgggatcgg cctatttctg acaagagacg aaagattgat
     1441 gtcggactat tttcagtgga accattactt actggattac attatagaag aatctatatt
     1501 taatcctcct tctccaatcc agaacgacac aaggtatccc tacctgttgc tttcgtcatt
     1561 aaacgacagc ttcgcgacag aatccacaca ctctactttt tcgtttcctc tttgaggcta
     1621 aaataagcca agcagagctg caaacgttcc acgaagaaca tttgcggctt gtttttatgc
     1681 ctacttattt actttgcctt gtgaaaatat acagaattta ccggaaaaat ataacacatc
     1741 tgttatattt tatagaggtt taaaatctac aaaattctga taatcaagaa aatagcctaa
     1801 aataggtaat aggtaagaac tcctaccttc caaaatagac taataatact tgaatgtccg
     1861 ctggagagac acctgagatg cgggaagcct gagctattgt acgcggacga tatctgttga
     1921 gctttattcg acactccatg gacaaagaag tgacttttga aaagtcaaag tcatccggaa
     1981 ttcggagatt ttcgaggcgt cgtatttttt cagctatatt tttttctcga tctatataac
     2041 ctttatattt aatactaatc tcagctgatt tgagtatctc ttcagataca atttcaggat
     2101 ttatatctcc tctaccctcc gttttgcccc aaggcatctc taaaagattt cttgtttgct
     2161 ctgtttcaat agatgatatt ttcgataatt tttctgataa attagaatct ataattgttc
     2221 cacgtggaac atatttgaga agagtgctaa tagatgtgga aggacgggat agaagatctg
     2281 aaatcttttt cttgtgtgac aatagggcgc tgtcaatgga acttaggtaa tcattgacat
     2341 cttccggact taaggtagta ctattagcaa agtcaatata gttcgaaatt aaatcatact
     2401 tcagtcgtgt aaactggtat ctgaattgat ctacaaggcc taattgatat cctttctcag
     2461 taagtctgct gtctgcatta tcctgtctta aaagaatacg gtattctgcg cgcgaagtaa
     2521 acattctata aggttcgtcc acgcctttcg taattaaatc atctatcagg accccgatat
     2581 aagcctcatc ccgctttagt ataaagggct ccttgttatt gagtttaaga tgggcattta
     2641 ttccagccat caatccttgg gctgccgcct cttcataacc ggtagtgcca ttgacttgtc
     2701 cggcaaaata gaggtttgga acaatcctgg actcaagagt gggatgaagc tctgtagggt
     2761 caaaaaagtc atattctaca gcataccccg gcgtaaagac tttaacaccg cgcaatccat
     2821 caatctcttg cagggcatca atctgtacgg atagaggaag cgaagatgca aagccctgga
     2881 gataatattc gtgggtattc cagccctccg gctctaaaaa taattgatga gaatctttgc
     2941 cggcaaaggt tctgagttta tcttcgatag agggacaata acgagggcct atacctttga
     3001 tctttccgct gaaaagtgga gattgctcaa aaccggtacg gagaatctca tgcacattct
     3061 tatttgtatg tacaatatag cagggtttct gctccttgcc tctctgaata cttgtcttaa
     3121 atg
//
LOCUS       DAOSIE010000169         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_27214_length_3113_cov_3.801504, whole genome shotgun sequence.
ACCESSION   DAOSIE010000169 DAOSIE010000000
VERSION     DAOSIE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27214_length_3113_cov_3.801504"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLI69_05395"
     CDS             complement(<1..309)
                     /locus_tag="PLI69_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOG41955.1"
                     /translation="MEYIIIIISAVFVNNIVLSQFLGVCPFLGVSKKVSTALGMSAAV
                     TFVMALSTLVTYLLQYYVLLPLGIGFLQTVSFILVIAGLVQMVEIILKKISPALYQAL
                     G"
     gene            complement(312..899)
                     /locus_tag="PLI69_05400"
     CDS             complement(312..899)
                     /locus_tag="PLI69_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOG41956.1"
                     /translation="MGKLKLISEGIIKNNPTFVLILGMCPTLGTTTSAINGMGMGLAT
                     LFVLVLSNMSISAIAKLIPEEVRIPSYIVVIAAFVTILQLLLQAFIPSLYETLGIFIP
                     LIVVNCIVLGRAEAYANKNSVIDSAFDGLGVGLGFTIALTILGLVRELLGSGSLFGWK
                     FIQGEGMLAFVLAPGAFIVLAYLIVLFRKVTANKA"
     gene            complement(902..1435)
                     /locus_tag="PLI69_05405"
     CDS             complement(902..1435)
                     /locus_tag="PLI69_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOG41957.1"
                     /translation="MVITLSVICLVCSALLGVVYSVTKAPIEAAELAKINNAIKAVVP
                     ECDNNPSEEVFEVDGSVVYPAKMNGTIVGYAIKVKASGFGGPIQMMVGFNAEGTIYNT
                     SVISHSETPGLGAQISDEIPTRTQIVGKNPASSKLSVKKDGGEIDAITASTITSRAFL
                     KGVDAAYKVFVELQKQQ"
     gene            complement(1455..2456)
                     /locus_tag="PLI69_05410"
     CDS             complement(1455..2456)
                     /locus_tag="PLI69_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOG41958.1"
                     /translation="MARLLVSGAPHLHVKESTQSLMRDVLIALAPSLLVSIYFFGFSA
                     IKLVLVGVISSVFVEYVIQKFILKTKVTINDYSAAVTGCLLALNLPPSSPWWLVFIGA
                     IVAIGIVKMTFGGLGQNIFNPALVGRVFLLISFPTLMTDWSIPESWFRDGVDAFTGAT
                     PLAITKEGLSAGLTLPEIFAANEFTFREMIFVKIGGSVGEVSALALILGFVYLLIRKV
                     IRPHIPLVIVGTIAVMTWIFNLINPDIYTGPLFNIFTGGVLLGAIFMATDYVTSPMSV
                     KGMVIYAFGIGVITVLIRYFGAYPEGVSFAILIMNMTVPLLNKYCKPKRYAKEVKHG"
     gene            complement(2460..>3113)
                     /locus_tag="PLI69_05415"
     CDS             complement(2460..>3113)
                     /locus_tag="PLI69_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HOG41959.1"
                     /translation="SLKKKYPQGGEKQLIEALTGRRVPSLALPIEVGAVVHNVGTALA
                     VYEAVQKNKPLIDSIITVTGTCVPHQRNFIVRVGTPFSKMLDAIGGLPKDAAKLVCGG
                     PMMGKAVANLEASTLKATSALLLLTEAETKRKEESNCIRCAKCTEACPMGLEPYLLNK
                     LSRIGGLYDELEQEKVHDCIECGCCLYSCPAHIPLLDIIRVSKAEVLTIMRNRSLKK"
BASE COUNT          890 a          716 c          740 g          767 t
ORIGIN      
        1 acccagtgct tggtaaagtg ctggactgat ctttttgagt ataatctcca ccatctgtac
       61 aagacctgca atcaccagta tgaaactcac cgtctggaga aatcctatac caaggggcaa
      121 taatacatag tactgaagca ggtaggtaac cagggtggaa agagccataa caaaggtcac
      181 cgcagccgac atgccgagag cggtacttac cttcttggag acaccgagaa agggacatac
      241 gccaaggaat tgcgacaaaa ctatgttgtt gacaaatacg gcggatatga tgataatgat
      301 gtattccatg attatgcctt gttagcggtt acttttctaa acaatactat gaggtatgcg
      361 agcacgataa atgcgcccgg agcaagtacg aaggcgagca ttccctctcc ttggataaat
      421 ttccagccaa agagcgaacc gctgccgagg agttcacgta caagaccgag aatggtcagg
      481 gcaatggtga aaccaagccc caccccgagt ccgtcaaaag cggagtctat cacggagttt
      541 ttgttggcat aggcttcggc acgaccgagt acgatacagt tgaccacaat cagcggaata
      601 aagataccga gagtctcgta aagtgagggt atgaatgcct gcaaaagcaa ctgcaatatg
      661 gtaacgaatg ccgcaatcac aacgatgtaa gagggtattc gcacctcttc gggaataagt
      721 ttcgcaatcg cagagatgga catattggag agcacaagca caaaaagcgt ggcaaggccc
      781 atacccattc cgttgatagc cgacgttgta gtgcctaatg taggacacat accgaggatg
      841 agaacaaacg tgggattgtt tttgattatg ccttcagata tcagttttaa ttttcccatg
      901 gttactgctg tttttgaagt tcaacgaata ccttgtaagc cgcatctaca cctttgagga
      961 aagccctcga agtgatagtt gaagcggtaa tggcgtcaat ctctcctcca tcttttttga
     1021 cggaaagctt ggaagaagcg ggattcttac ccacgatttg agttctggtg ggaatttcat
     1081 cagaaatctg ggcgccgaga ccgggagttt ccgagtggga aatcacagac gtattgtaga
     1141 ttgtaccctc tgcattgaag cccaccatca tctggatggg accaccaaaa ccggatgcct
     1201 ttacctttat ggcgtatccg actatggtgc cgttcatttt tgcaggatac actacactgc
     1261 catccacctc aaagacctct tccgagggat tattgtcaca ctcagggacg acggctttga
     1321 tagcgttgtt gattttggcc agttccgcag cctctatggg agcttttgtc accgaataga
     1381 caactccaag aagtgcagag cataccagac agataaccga cagagttatc accatacttt
     1441 tgaaagagga ttccttagcc atgtttcacc tcctttgcat atctcttggg tttacaatat
     1501 ttatttaaga gagggacggt catattcata attagaattg cgaaagagac tccctccgga
     1561 taggctccaa aataacggat aagcaccgta atgacaccaa taccaaaggc atatattacc
     1621 atacctttga cggacatcgg cgaggtaaca tagtcggtcg ccataaagat agctccaagc
     1681 agtacacctc cggtgaagat attaaagaga ggacccgtgt agatatcagg attgatcaaa
     1741 ttgaaaatcc aggtcatcac agcgatggta cccacgataa caagcggaat atgaggtctt
     1801 atgactttcc ttatgaggag atagacaaat ccgagtataa gggcaagtgc agaaacctct
     1861 cctaccgacc ctccgatctt gacgaaaatc atctccctga atgtaaactc atttgcagca
     1921 aaaatctcgg gcagggtaag tcctgcagag agaccctctt ttgtgattgc aagtggagtt
     1981 gctcccgtaa aggcatcaac accgtctcta aaccaagatt cgggaattga ccagtcagtc
     2041 attaatgtag ggaatgagat aagcaggaat acgcggccca caagagccgg attgaatatg
     2101 ttctgtccca gaccgccgaa agtcattttg actatgccta ttgcgactat tgcacctata
     2161 aagacaagcc accagggaga gctgggcgga aggttgagag ccaaaaggca accggttact
     2221 gccgcagaat aatcatttat ggtaactttt gtcttaagta tgaatttttg aattacatat
     2281 tccacaaaaa cagaggaaat tacacccaca agcaccagtt tgattgccga gaatccgaag
     2341 aaatagatgg aaaccagcag tgatggcgcg agggctatca gtacatccct catcagagac
     2401 tgtgtactct cctttacatg gaggtgaggg gccccggata ctagaagtct tgccatagtt
     2461 tactttttaa gggatcggtt acgcattatt gtcaaaacct ctgccttgct gaccctgatt
     2521 atgtcaagca gaggaatgtg tgcaggacag gagtagaggc aacagccaca ctcgatgcag
     2581 tcatgtactt tctcttgttc aagttcatca tacaacccgc ctatacggga gagtttgttg
     2641 agaagatagg gctccagtcc catcggacag gcttcggtac attttgcgca tctgatgcaa
     2701 ttgctctctt ccttcctctt ggtttcagct tcggttaaaa gcagtagtgc agaggttgcc
     2761 tttaaagtgg atgcctccag atttgccaca gctttcccca tcatggggcc tccgcagacc
     2821 agcttggccg catccttcgg caggccaccg atggcatcga gcatcttgga gaatggagta
     2881 cctaccctga cgatgaaatt tctctgatgg gggacacaag taccggtgac ggttataata
     2941 ctatcaataa gaggtttgtt tttctgaacc gcctcatata ctgcgagtgc agtacccaca
     3001 ttatgaacga ccgctccaac ttcgatagga agagccaagg aggggactct gcgaccggtc
     3061 agggcctcga taagttgttt ttcaccgccc tgagggtatt tcttctttag aga
//
LOCUS       DAOSIE010000170         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_27317_length_3105_cov_3.949508, whole genome shotgun sequence.
ACCESSION   DAOSIE010000170 DAOSIE010000000
VERSION     DAOSIE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27317_length_3105_cov_3.949508"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PLI69_05420"
     CDS             <1..1027
                     /locus_tag="PLI69_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG41960.1"
                     /translation="TYDAMGICTTGKKPIYIYSRGRFEDGNNIWMTSDNFNTQVQVTN
                     ANPQQRDYNWGTVELVSWNTEDGIRAEGLLFKPENFDPSKKYPLMIYFYEKYSDDLYA
                     PRTPSPSRSTVNIPYFVSNEYIVFVPDIYYTDGHPGQSAMKSIMPACDMLCENPWIDG
                     DNMAIQGQSWGGYQVAYMITQTNRFKAAGSGAPVSNMTSAYGGIRWESGMARTFQYEQ
                     QQSRIGKDLWEGFDLYIENSPLFFVPNVTTPVLIMHNDEDGAVPWWQGIEFFNALRRC
                     GKEAWMLQYNGEAHNLRERRNARDLSIRLEQFFDHYLKGKPAPVWMSKGVPATLKGID
                     LGYGYEQ"
     gene            1055..>3105
                     /locus_tag="PLI69_05425"
     CDS             1055..>3105
                     /locus_tag="PLI69_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG41961.1"
                     /translation="MKRLLTIISIMIAISSATSCKNASSEADPASAPLITKPEVVVQD
                     GHFTPEIMHSLAKVSDPQISPDGTKILYGVGYTSIEQNKSNRELFVMNIDGSNKVQLT
                     TTPQSENNARWIEDGKRIAFLRGGQMWVMNSDGTGEKQVSDIPDGMMEFKLSPDGKRV
                     LYSANFKVVKSPAEIHPNLPKSTARTIEGLMYRHWDHFVETIPHTYLADFDGNTLGEG
                     VDLLEGAPYELPAEPFGGIEQLDFSPEGDRIVYSCRKKTGREYAFSTNSDIYLLNLSD
                     NNCVNISEGMMGYDTNPVFSPDGGAIAWMSMERDGYEADKIRLFVYEITSGKRRELSA
                     SFKYNIENPVWMPDSKGLYFTSLVEGLQELWMTDLEGNMSRVTPEREWYNFGAPSLLT
                     STAEDGSVKVERLITTNLCMLRPAEIVSINPQDGSWEQLTFENKYIFDQLDEVTVEER
                     WIRTSDNKKMLTWVLYPPIFDKSKVYPSILLCLGGPQGTLSQGWSYRWNYRLMASQGY
                     IVVLPNRRGTTAFGQEWCEQISGDYIGQNMRDYFAAADALKAEPYVGKMAAVGASYGG
                     YSVFYLAGIHQKRFDAFIAHAGIFNVEHMYMTTEEMWFPDWDNGGIAQPDTYMAGSPW
                     SNNPVAKRHYANSPHKLVEKWDTPILVTHGELDYRVPVDQGMAAFNAAQMMGVPSRMI
                     L"
BASE COUNT          816 a          755 c          762 g          772 t
ORIGIN      
        1 tacctacgac gccatgggta tctgcactac gggcaaaaag cccatctaca tctactcaag
       61 gggacgtttt gaggatggca acaatatctg gatgacctcc gacaatttta acacacaggt
      121 gcaagttaca aatgcaaatc cccagcagag agattataac tggggcactg tggaacttgt
      181 ttcctggaac accgaagatg gaataagagc tgaaggactt cttttcaaac ctgagaattt
      241 cgatccttca aagaaatatc ctttgatgat atatttctac gaaaaatatt cggatgatct
      301 ctatgcgccc cgtacacctt caccgagccg ttcgaccgtc aacattccct actttgtaag
      361 caatgagtat atcgtgtttg ttcccgatat ctattatact gacggacatc ccggccagag
      421 cgccatgaag tcaattatgc ccgcttgcga catgctctgt gaaaatccct ggatcgatgg
      481 cgacaatatg gccattcagg gtcaaagctg gggcggatat caggtggcct atatgattac
      541 acagaccaac cgcttcaagg cggccggctc cggtgctccc gtttccaata tgacgagcgc
      601 atacggcggc atccgctggg agagcggtat ggcgcgtaca ttccagtatg agcaacagca
      661 gagtcgtatc ggcaaggatc tttgggaggg ctttgacctc tatatcgaaa attcacccct
      721 cttctttgta ccaaatgtga ccacccctgt actcatcatg cacaatgacg aggatggcgc
      781 agtcccctgg tggcagggga tcgaattttt caatgccctc agacgctgcg gcaaagaggc
      841 ctggatgctt cagtacaatg gtgaagctca caacctacgc gagcgccgca acgcacgtga
      901 cctcagcatc cgcctggaac aatttttcga tcactatctg aagggaaaac ctgctccggt
      961 ttggatgagc aaaggcgttc ccgcaactct caagggcatc gacctcggat atggatatga
     1021 acaatagtat ttacaatata taattttaaa aatcatgaaa cgtttattga caattatatc
     1081 gattatgatt gcaatttcat cagcgacatc ctgcaaaaat gcttcaagcg aggctgatcc
     1141 ggcctctgct cccctcatta caaaacctga ggtggtggtg caagatggcc atttcacacc
     1201 tgaaatcatg cattctctgg ccaaagtctc cgatcctcaa atctcccccg atggcacaaa
     1261 aattctctac ggggtaggtt atacctccat cgaacagaat aagagcaacc gtgaactttt
     1321 tgtgatgaat attgacggaa gcaataaggt acagcttaca accacccctc aaagtgagaa
     1381 taatgctcgc tggatagagg atggaaagcg cattgccttt ctcagaggcg gtcaaatgtg
     1441 ggtaatgaat agtgacggca ccggagagaa gcaggtaagt gatattcccg atggaatgat
     1501 ggagtttaaa ctctctccag acgggaaaag agtactctat tccgccaact tcaaggtggt
     1561 aaagagccct gcggagatac atccgaatct gccaaagtcc acggcccgca ctatcgaggg
     1621 gctgatgtac agacactggg accatttcgt agagactatt cctcatacct atctggccga
     1681 tttcgacgga aatactttag gagagggagt tgacctcctt gagggggctc cttacgagtt
     1741 acccgccgag ccctttggcg gcatcgagca gctcgatttt tctcctgagg gtgatcgcat
     1801 tgtctattcc tgccgcaaga aaaccggcag ggagtatgca ttctctacca acagcgacat
     1861 ttatctgctc aatctttcgg acaataactg tgtaaatatt tccgagggca tgatgggcta
     1921 tgataccaat ccggtattca gccccgatgg aggtgccatt gcctggatga gcatggagag
     1981 ggatgggtat gaggctgata agattcgtct tttcgtatat gagattactt ccggcaagcg
     2041 ccgtgaactc tctgcttcct ttaaatacaa cattgagaat cccgtatgga tgcccgactc
     2101 caagggactt tatttcacct cgttggtgga ggggctgcag gagctatgga tgacggacct
     2161 tgagggcaat atgtccagag taacccctga acgcgagtgg tataatttcg gtgcgccctc
     2221 actgctgact tctaccgctg aagatggttc agtcaaggtg gagcgcttga ttaccactaa
     2281 tctatgtatg ttgcgccctg ctgagattgt ttccatcaac ccacaggacg gcagctggga
     2341 gcagctcact ttcgaaaaca agtatatctt cgatcaactc gatgaagtga ccgtggaaga
     2401 gaggtggatc cgtacttcgg acaacaaaaa gatgcttacc tgggtactct atcctcccat
     2461 atttgacaag agcaaagtat atccttcgat tctgctatgc ctcggaggtc ctcagggaac
     2521 attgagccag ggctggtcct atcgttggaa ttaccgtctg atggcctctc agggctatat
     2581 cgtggtgctt cccaacagac gcgggacgac tgcctttggc caggagtggt gtgagcagat
     2641 ctcaggtgat tacatcggcc agaacatgag agattatttt gctgcggctg atgctctcaa
     2701 ggctgagccc tatgtgggca aaatggcagc agtgggtgct tcctacgggg gatattccgt
     2761 gttttatctt gcgggaatac accaaaaacg ctttgatgca ttcattgcgc atgccggtat
     2821 tttcaatgtg gaacacatgt atatgaccac cgaagagatg tggttccccg attgggacaa
     2881 cggcggtatc gctcaacctg atacatacat ggccggatcc ccctggagca acaatcccgt
     2941 tgcaaaaaga cactatgcca actctcctca caaacttgtg gaaaaatggg atactcctat
     3001 ccttgttact catggagaac tcgattaccg tgtgccggta gaccagggaa tggctgcatt
     3061 caatgctgca caaatgatgg gagttccctc cagaatgatt ctttt
//
LOCUS       DAOSIE010000171         3101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_27360_length_3101_cov_3.796126, whole genome shotgun sequence.
ACCESSION   DAOSIE010000171 DAOSIE010000000
VERSION     DAOSIE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27360_length_3101_cov_3.796126"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLI69_05430"
     CDS             complement(<1..790)
                     /locus_tag="PLI69_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41962.1"
                     /translation="MTNLFGTAADDRGSLCFFSYSVGGGMRQFSGFSYQVSRLDDGST
                     EILINEDFPDQKRIIISDQAVFSELQAIIDQYNMTSYKGRYEPPLQVLDADSWHFDLK
                     YEYGFTVSASGTQRYPRGGAAAFKALKEYFKKWHDMPSEVDNIVSFDYTCVTGKKDRI
                     SYKVSSGEEGITMHLVDVKQGFDEKLRPNPSIFEKIRKCLIVYNMDTPDAYTYFNKKE
                     QGQWSFDVKYASGEHLALKGSYGDNKGGFIDALESIFQDWLPVKG"
     gene            complement(910..>3101)
                     /locus_tag="PLI69_05435"
     CDS             complement(910..>3101)
                     /locus_tag="PLI69_05435"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOG41963.1"
                     /translation="GSVVVKMGKTMLLAAVTCAKEAKEDVDFMPLQVEYKEKYAAAGR
                     YPGGFMKREGRPSDYEILVARLIDRALRPLFPEKFHAEVFVTVNLISADGVDMPDALA
                     GLAASAALAVSDIPFEGPISEVRVARIDGKFCINPTFEQLNKADMDIIVAATYENIMM
                     VEGEMKEVSESDMLEAIKFAHAEIKKHCLVQKELEKEVGKEIKREYSHETDDEVLDKA
                     AWEYCYDRCYAIAKSGSDKHTRTDQFEALHQEFLETLPEEEREEKAIMVARYFNAIEK
                     KAMRRMILDEGIRLDGRKTTDIRPIWCEVDYLPGAHGSAIFTRGETQSLTTVTLGTKD
                     DMKERDVVLTQGTDQFVLHYNFPPFSTGEAKPSRGVSRREVGHGNLAWRALKEVVPVG
                     EDNPYAVRVVSDILESNGSSSMATVCAGSLALMDCGVKISKPVSGIAMGLISDSESSK
                     YAVLSDILGDEDHLGDMDFKVAGTKDGITATQMDIKVDGLSYEVLEKALEQARQGRNH
                     ILNEMLKTISEPRAEYKPHVPRIIQIVVPGEFIGAIIGKGGEVIQDIQKQTETSITIT
                     ESKDEKGEPIGMVDIFGIDKEKMDAALARIKAIVTVPEVGEVYKGKVVSIVEFGAFVE
                     ILPGKDGLLHISEMDWGKTEKVEDLFQVGDEVEVKLIEIDPKTNKLRLSRRALLEKPE
                     GYVEPVRRPRPSTPRPHSGHSGHGGGDRSDRGDRKGGFRKDFNKGKK"
BASE COUNT          792 a          804 c          649 g          856 t
ORIGIN      
        1 tacccttaac cggtagccag tcctgaaaaa tactctcaag cgcatcaata aatcctccct
       61 tgttatctcc ataactgcct ttcagggcga gatgctctcc ggaagcatat ttcacatcga
      121 aagaccattg tccctgctct ttcttgttga aataagtata ggcatcagga gtatccatat
      181 tgtagacgat aagacatttg cgaatttttt caaatatgga tgggttaggc ctcaatttct
      241 cgtcaaaacc ctgttttaca tcgacaaggt gcatggtaat accctcttcg ccgctgctta
      301 ctttataact gatcctatct ttctttccgg taacgcaggt ataatcaaac gatacaatgt
      361 tatctacttc agacggcata tcatgccact ttttaaaata ctctttcaaa gccttgaaag
      421 cggctgctcc gccacgagga tacctttgag taccacttgc actaactgta aaaccgtact
      481 cgtatttaag gtcaaaatgc catgaatcgg catccaaaac ctgtagaggt ggttcatacc
      541 tccctttata tgaggtcatg ttgtactgat caatgattgc ctgcagctca ctgaacacag
      601 cctgatccga gataatgatc cttttctgat cagggaaatc ctcattaatg agaatttctg
      661 tgctgccgtc atccaagcgg gagacctgat atgaaaaacc ggaaaattgt ctcattccac
      721 ctcctacact ataagaaaaa aagcacaaag agcctctatc atctgcggca gtaccaaaca
      781 aattcgtcat aacaagcaat agtattaata ataacctttt catacaccct ctacggacaa
      841 agataggaaa aaagtgtata gtgtgagtat tttcggaatt aaaaaacggc ccttgagggc
      901 cgttttctac tatttctttc ctttattgaa atcttttcgg aagccgcctt tgcggtcgcc
      961 acgatcgctg cgatcaccgc cgccgtgacc gctgtggccg ctgtgagggc gaggggttga
     1021 aggacggggc ctgcgcacag gctccacata accttcgggt ttctccaaaa gggctctgcg
     1081 ggaaagacga agcttattgg ttttaggatc gatctcgatg agttttactt ctacctcatc
     1141 accaacctgg aagagatcct caaccttttc cgttttaccc caatccatct ctgaaatatg
     1201 gagcagacca tccttgccgg ggagaatttc cacaaatgca ccaaattcta cgatagatac
     1261 tactttacct ttgtagactt cacccacttc gggaaccgta acaatggctt tgatacgtgc
     1321 cagtgctgca tccattttct ccttatcaat tccgaaaata tccaccatgc cgataggctc
     1381 gcccttttcg tccttggact cagtgatggt gatgcttgtc tcggtttgct tctggatatc
     1441 ctgaataacc tcaccgcctt tacctataat tgcgcctata aactcaccgg gaaccacaat
     1501 ctgaataatg cgtggcacat ggggcttgta ctctgcgcga ggctccgaga tagttttaag
     1561 catttcattc aaaatatggt tgcgaccctg gcgtgcctgc tcaagagcct tttcaagtac
     1621 ctcataagag agaccgtcaa ccttgatgtc catttgggta gctgtaatac catctttggt
     1681 gccggcaacc ttgaagtcca tatctccgag gtggtcctcg tcaccgagga tatcggaaag
     1741 aactgcatat tttgaagact cactatctga aatcaaaccc attgcaatac ctgaaacggg
     1801 cttggaaatc ttcacgccgc aatccatgag tgcaagagaa cccgcacata cggtagccat
     1861 cgaagaggaa ccgttggact ccaggatatc cgacactacg cgtactgcat agggattatc
     1921 ttctcctacc ggaacaacct ctttaagcgc cctccaggca aggtttccgt gacccacctc
     1981 tctacggctc acaccgcggg atggttttgc ttcaccggtg gaaaagggag ggaagttgta
     2041 gtgtagcaca aactgatcgg taccctgagt gagtactacg tctctctcct tcatatcgtc
     2101 cttagtgccg agagtaaccg tagtaagtga ctgagtctca cctctggtga aaatagctga
     2161 gccgtgagca ccggggagat agtccacctc gcaccagata gggcgaatat ctgtggtttt
     2221 gcgaccatcg agacggatac cttcgtcgag gatcatacga cgcatagcct tcttctcgat
     2281 tgcattgaag taccttgcaa ccatgatggc cttctcctcc ctctcctctt cgggcagggt
     2341 ctcgagaaac tcctggtgaa gcgcttcaaa ctgatcggta cgggtatgtt tgtcgctacc
     2401 gctcttagcg attgcataac atctgtcata gcaatattcc caggcagcct tatcaaggac
     2461 ttcgtcatct gtttcgtgac tatattcacg tttgatctct ttgcctacct ccttctccag
     2521 ttctttctgc acaagacagt gcttcttgat ctctgcgtga gcaaatttta tggcttccag
     2581 catatcagac tcgctaacct ccttcatttc accctctacc atcattatat tttcgtaggt
     2641 ggcagccact attatgtcca tatcagcctt attcagctgc tcaaaggtag ggttgatgca
     2701 gaatttacca tctatgcgtg caacgcgcac ctctgagatc ggaccttcaa aaggtatgtc
     2761 actcacggcg agggcggctg aagcggcaag tcctgcaagt gcatcaggca tatcaacacc
     2821 atcagccgag atgaggttga cggtaacaaa gacctctgcg tggaattttt cgggaaaaag
     2881 tggacggagc gccctgtcaa taaggcgtgc taccagtatt tcataatctg agggtcttcc
     2941 ctctctcttc ataaaaccac ccggataacg gcccgctgcg gcatattttt ctttgtattc
     3001 aacttgaaga ggcatgaaat caacatcttc cttggcctct tttgcgcatg taacggcagc
     3061 caacaacatg gtcttgccca tttttactac tactgagccg t
//
LOCUS       DAOSIE010000172         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_27553_length_3086_cov_3.657209, whole genome shotgun sequence.
ACCESSION   DAOSIE010000172 DAOSIE010000000
VERSION     DAOSIE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27553_length_3086_cov_3.657209"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLI69_05440"
     CDS             <1..804
                     /locus_tag="PLI69_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41964.1"
                     /translation="GGVTVAERHLFLNKMEELSGMPPAQVAEEYDMFIKGFISNLESE
                     KSVVLPSLGRMHATSTNEYFFVADEDLDIYPDGIGLEPVSIKAPDPDFVPVAPPEPLP
                     KPELEVEQDKTAALDPEVTGESEGEVDEHTGVETQAEQKPESESQTDSESIDTVLIDP
                     ETIEDNEFVEVSGAETKSEPEPELVAEPDAEPVAEPPSDPKPWNEKDSSDFEIEPSKK
                     RVGLWVVVIIIALVMVGALLLYIFRDSEWLSPLLDKILYSKEELRILGR"
     gene            811..2847
                     /gene="dnaG"
                     /locus_tag="PLI69_05445"
     CDS             811..2847
                     /gene="dnaG"
                     /locus_tag="PLI69_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOG41965.1"
                     /translation="MIDRDTINRILDAVKIEEVIGDFVSLRRKGSNWWGLCPFHPDRN
                     PSMAVSSTKNLFKCFSCGKGGNALSFVMEHENMSYPEALRYLAAKYNIEVVDKELTPE
                     EVARNQRYDNLLVVSDFAQKFYQDLLWNNDFGRTVGLSYFRQRGYTDDTIRKFGLGFA
                     SHHPDTLIDGARKEGYKEEYLIGATLCVARENGEVSDRFYDRVMFPIHSISGRVIAFG
                     GRTLKKDKNVPKYVNSENSEIYKKSDAVYGIFFAKSAIAKEKKCYLVEGYTDVVSMHQ
                     AGITNVVAPCGTALTAGQIRLMKRFSPDVTIIYDNDEAGINASLKNINLLLEEGMNVR
                     VLLLPEGEDPDSFAMAHTGDEIRDFIENNEEDCISYKCRLLVDSERDPVRKSRAIRDV
                     IETIAYVSDPVLRNLYVDNLSKKYDQKPEMLFKEISTLRRKWREERQRMEQRRGMEVS
                     SGHLRPVYEGQQTPPASEEYYEPAGDLLIGDVYSSNTPMDVSERELLYYLVKFGCYPI
                     HFVEDMLYGAQKREGVTVADYIRSALSEDELEFENPLYRAIYEEFYSLYGTLEGSLDP
                     EEQQKQVVRFLSAHNNDSLRKTVLGLLLETHTLTVKEYRLSIAPEETILGVTVPKSMV
                     LYKLRITEQACIDITKEIAAAEANGDLERQKALMMQLQMFNQVKNRFAKELNRL"
     gene            2897..>3086
                     /locus_tag="PLI69_05450"
     CDS             2897..>3086
                     /locus_tag="PLI69_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG41966.1"
                     /translation="MNTNYKRNLVTCALPYANGPIHIGHLAGVYVPADIYVRYLHMRG
                     RKTLFVCGSDEHGVPITIK"
BASE COUNT          865 a          610 c          767 g          844 t
ORIGIN      
        1 ggtggagtaa ctgttgccga gagacatctt tttcttaata agatggagga gctttcaggt
       61 atgcctcctg cgcaggtcgc tgaagagtat gacatgttta tcaaaggttt tatatcaaac
      121 cttgagagtg aaaaatcagt agttttacct tcgctcggtc gtatgcatgc cacatcgacc
      181 aatgagtatt ttttcgttgc agatgaagat ttggacatct atcctgatgg tattggtctt
      241 gaacctgtct ccattaaagc tccggatcct gattttgtgc cggtagctcc tcccgagccg
      301 ctgcctaaac ctgaattgga agttgagcaa gataaaaccg cagcacttga tcctgaagta
      361 acaggagaaa gtgagggtga ggtggatgaa cataccggtg ttgaaactca agccgaacaa
      421 aaacccgaat cagagtccca aacggattca gagagtattg atactgtcct aatagaccct
      481 gaaacgattg aagacaacga gtttgttgag gtgtccgggg cagaaaccaa gtctgaaccg
      541 gaaccggagc tcgtagcgga gcctgacgca gaaccggtgg cggaaccacc atctgatcct
      601 aaaccatgga acgaaaaaga ctcttctgat tttgaaatcg aaccctccaa aaaacgtgtg
      661 gggctttggg tggttgtgat aattattgct ctggtgatgg taggggctct tttactctat
      721 atattcagag actctgagtg gctttcacca ttactagaca agattcttta ttccaaagag
      781 gaacttagaa ttctaggcag ataacttaca atgattgaca gagataccat aaacagaatc
      841 ctcgatgcag taaagatcga ggaggtaata ggcgatttcg tttcacttag acggaaaggc
      901 tccaactggt ggggtctctg tccatttcat cctgatagaa atccctcgat ggctgtttct
      961 tcgaccaaaa atctcttcaa gtgcttttct tgcggcaagg gtggaaatgc cctctccttc
     1021 gttatggagc atgagaatat gagttacccc gaagcactac gctatctggc ggcaaagtac
     1081 aatatcgagg tggtggataa ggaacttaca cctgaagagg tagcccgtaa ccagcgctac
     1141 gacaatcttc tagtagtgag tgatttcgca caaaaatttt accaggatct gctctggaac
     1201 aatgattttg gaaggactgt gggactctct tatttcaggc agagaggcta tactgatgat
     1261 accatccgta aattcggtct tggctttgct tcgcaccacc ctgatactct gattgatggt
     1321 gcccgtaaag agggctacaa ggaggagtat ctgatcggtg ccactctttg cgttgcccgg
     1381 gaaaatggtg aagtgagcga cagattttat gatagggtga tgttcccgat tcactccatc
     1441 agtggcaggg tcatagcctt cggtggccgt actttgaaaa aagataagaa tgtccccaaa
     1501 tatgtaaata gcgagaatag cgaaatatat aaaaagagtg atgccgtata tggtatattc
     1561 ttcgcaaaat cggccattgc taaagagaaa aaatgttatt tggtagaggg atatacagat
     1621 gttgtctcga tgcaccaagc aggtataaca aatgtggtgg caccctgcgg tactgctcta
     1681 acggcgggtc agattcgcct tatgaagcgc ttttcacccg atgtgactat tatttatgat
     1741 aatgatgagg cgggtataaa tgcttctctc aagaatatta atctcctttt ggaagagggt
     1801 atgaatgtca gggtactgtt gttgcccgaa ggtgaggatc cggatagctt tgctatggcg
     1861 cacacgggtg acgagattag ggatttcatt gaaaataatg aggaggattg tatttcctat
     1921 aagtgccggc tattagtgga tagtgagcgt gatcccgttc ggaagtcaag ggcgataagg
     1981 gatgtgatcg agaccattgc ttatgtatcg gatcccgtct tgagaaatct ctatgtggat
     2041 aatctctcta aaaaatatga tcagaaaccg gaaatgcttt tcaaggaaat atctacttta
     2101 cgtcgaaaat ggcgtgagga gaggcagcga atggagcagc gcagggggat ggaagtctct
     2161 tccggacacc tgagacctgt ttacgagggt caacagacgc ctcctgcttc tgaagagtat
     2221 tatgagccgg ccggtgacct tttgatcgga gatgtttata gcagtaatac tcctatggat
     2281 gtttctgaaa gggagcttct ctattatttg gtaaaatttg gctgctatcc gattcatttt
     2341 gttgaggata tgttgtatgg tgcgcaaaag agggaaggag tgactgtcgc cgactacatc
     2401 cggtctgcgc tcagtgaaga tgaactcgaa tttgaaaacc ctctctatcg tgcaatttat
     2461 gaggagtttt attctctgta cggcacgctt gagggaagtc tcgatccgga agagcaacag
     2521 aagcaagttg tacgcttttt atcggctcat aacaatgatt cgcttcgtaa aacggtgctg
     2581 ggattgcttt tagagactca taccttaaca gttaaggaat atcgcttgtc aattgcaccg
     2641 gaagagacaa tacttggagt taccgtaccg aaatccatgg ttttatacaa attgcgcatt
     2701 accgaacagg cctgtattga tattactaag gagatagctg cggccgaagc aaatggtgac
     2761 ctggagcgcc agaaagccct catgatgcag ctccaaatgt tcaatcaggt gaaaaatcgc
     2821 tttgctaaag agcttaaccg cctctgattg caaatatttc actaaatttg tcgctcaatt
     2881 ttaaaatttt aagaggatga atactaatta caaaagaaat ctcgtcacct gtgctttgcc
     2941 ttatgcaaac ggtcccatac atatcggtca ccttgcagga gtctatgtcc ctgctgacat
     3001 atatgtgagg tatcttcata tgcgcgggag aaagactctc tttgtatgcg gttcggatga
     3061 acacggagtt cccattacaa ttaagg
//
LOCUS       DAOSIE010000173         3085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_27561_length_3085_cov_3.587789, whole genome shotgun sequence.
ACCESSION   DAOSIE010000173 DAOSIE010000000
VERSION     DAOSIE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27561_length_3085_cov_3.587789"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLI69_05455"
     CDS             <1..395
                     /locus_tag="PLI69_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOG41967.1"
                     /translation="KYYSLIRPEPEYYKWFCQRVHGLSEEETSEAPVFPHVWEKIAPM
                     IEGLPLVAHNSSFDEGCLKEVFKVYQMDYPDYVFHDTCATSRKHFANSIDNHELQTVA
                     AACGFNLENHHHALADAEACAHIAMKIL"
     gene            complement(509..1771)
                     /locus_tag="PLI69_05460"
     CDS             complement(509..1771)
                     /locus_tag="PLI69_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HOG41968.1"
                     /translation="MNKVYVYADFDWLRSIELIGELGYESLRGSDCYSFNFADSWIKK
                     YHAINLSDDLNNYPGLQNTQPGKDIFGCFADSLPDRWGRTLLLRREQTMAQEENRPTR
                     RLSSFDLLMGIDDFSRMGGFRFKNTPDGDFINTEDSLRIPPLTKIPELIMASKEIEKS
                     EEINRLPEKQWILQLVNPGSSLGGARPKANVLDTDNTISIAKFPSLKDDYDAGLWEHF
                     CHIMAKNAGINAAQTRVYKFNDRHHTLLSRRFDRSDSGRRIHFASAMTLLGLNDGDGA
                     ATGHGYIDIVDFIIRGCSDVEANLRELFRRVAFNICVGNSDDHFRNHGFLLTAKGWRL
                     SPAYDMNPTQNSHQSLLINGTTNSADLSILLNSCEEYMLTTDVAKIIVDEVVDAVKGW
                     RVVANRLSIAKREIDLFEGIISERISRI"
     gene            complement(1777..2097)
                     /locus_tag="PLI69_05465"
     CDS             complement(1777..2097)
                     /locus_tag="PLI69_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG41969.1"
                     /translation="MTKNTMGTKLPRKLEQKMQIVGEQIKLARLRRDLSIAQVAERAT
                     CSPLTVSRIEKGTPTVAIGIYLRVLYALQLDEDILHLAKDDKLGRALQDINMRPRERA
                     SKKE"
     gene            complement(2316..>3085)
                     /locus_tag="PLI69_05470"
     CDS             complement(2316..>3085)
                     /locus_tag="PLI69_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOG41970.1"
                     /translation="KRLDEAAQGLLVINMGGTAIGTGITAEPGYAEACTKYIKKISGW
                     KVTLAEDLIEASNDTSAFVVYSSAIKNVAIRISKISNDLRLLSSGPRTGLGEINLPPK
                     QPGSSIMPGKVNPVIPEVVNQVCFKIIGNDVAITMASEAAQLELNVMEPVLVQSLVES
                     ATWLMRACDTLRVECVDGITANKEHCKKLVEYSIGIVTALKPYLGYAKITEVAQEAQK
                     TGGSVYKLVLEKGFLTKEQLDKIMDPANMTKPVKVEL"
BASE COUNT          820 a          732 c          642 g          891 t
ORIGIN      
        1 aaaaatacta cagcctcatc cgtcctgagc cggaatatta taaatggttc tgtcagcgtg
       61 tgcacggtct ttcggaagag gagacatccg aagcgccggt atttcctcat gtgtgggaaa
      121 agattgcgcc gatgattgag ggtctgccgc ttgtggcgca caactcctct ttcgatgagg
      181 ggtgtttgaa agaggtattc aaggtgtatc agatggatta cccggactat gtgtttcacg
      241 acacctgcgc tacctcccga aagcatttcg caaactccat agataatcac gaattacaga
      301 cggttgccgc tgcatgcgga ttcaaccttg aaaaccatca ccacgctctg gctgatgccg
      361 aagcctgcgc acatattgcc atgaagattt tataaagagt tttgagtttt tctcgtttga
      421 gttttaaaga taaattgtta gagtttctat tcgccgccgg ccgtgaggtt tgcggcgaat
      481 tttaaattgg tgatgtgaca cttgagaatt aaatccggct gattctttca gaaattattc
      541 cttcaaacaa atctatttct cgttttgcga tggacaatct atttgcaaca acacgccatc
      601 ctttaacggc atcaacaacc tcatcaacaa taatttttgc tacatcagtg gttaacatgt
      661 attcttcgca ggaattcaaa agtattgata ggtcggcact attggttgtt ccatttataa
      721 gcaggctctg atggctgttt tgtgtgggat tcatatcgta agcaggtgaa agcctccatc
      781 cttttgcagt aagcaaaaat ccgtggttgc ggaaatggtc atcgctattt ccgacacata
      841 tgttgaatgc aactctgcgg aataattcac gcaagttcgc ctcaacatcg ctacaccctc
      901 ttattataaa atccacaatg tcaatatagc cgtgccctgt tgccgcaccg tcaccatcgt
      961 tcagcccaag cagagtcatt gcagatgcaa aatgaattct tctgccgctg tcggatctgt
     1021 caaaacgacg cgagagaagt gtatgatgcc tatcattgaa tttatacacc ctcgtttgtg
     1081 ccgcatttat tcctgcattt ttagccataa tatgacaaaa atgctcccat aatcctgcgt
     1141 cataatcatc tttcaacgag ggaaacttag caatagatat agtgttgtca gtatccaaca
     1201 catttgcttt cgggcgggcg ccaccaagcg atgaacccgg attgacgagt tgtaaaatcc
     1261 actgtttttc cggaagccta ttaatttctt cactcttttc tatctctttg cttgccatta
     1321 taagctccgg aatctttgtc agtggcggta ttcgcaaaga gtcttcggta ttaataaaat
     1381 ctccgtccgg tgtattcttg aaacgaaagc ctcccattcg ggaaaaatca tcaataccca
     1441 tcaagaggtc aaatgaggaa agacgacggg tcggacgatt ctcctcttgt gccattgtct
     1501 gctctcgccg aagtaacaga gtgcgccccc atctgtcggg aagcgaatcc gcaaaacagc
     1561 caaaaatatc tttgcccggt tgtgtatttt gtaagcccgg ataattattc aggtcatcac
     1621 taagattgat ggcatggtat ttttttatcc aactatcagc aaaattaaaa ctataacaat
     1681 ctgatcctcg cagcgactcg tatccaagtt ctccaatcag ttctatactc cttagccaat
     1741 caaaatcggc atatacatac actttattca tagctactat tcctttttag aggccctttc
     1801 ccgcggcctc atatttatat cctgtaatgc tctgccgagt ttatcatctt ttgccaaatg
     1861 gagaatatct tcatcgagtt gcaatgcata tagcacacgc aaatagatgc ctatcgctac
     1921 tgtcggagtt cctttttcta tgcgtgaaac cgtaagcggt gaacaggtag ccctctccgc
     1981 cacctgcgca atactcaaat cacgacgcag ccgcgcaagc ttgatctgct cgccgacaat
     2041 ctgcatcttt tgctctaatt ttcgaggcaa cttagtcccc attgtgttct ttgtcataac
     2101 gataacatat tatataatat gcaaatataa cactatttcc atattttatg atatattaag
     2161 agaaatatta atcccttgca agcgtacgat gtgacacttg agctttgcac acatcaggca
     2221 acgcataacg cgctactcat aactacacaa aaaagagtcc ttgaagggac tcttttgtgt
     2281 actctgaact cctgattcgg aactcaaaac taactttaca gctccacctt aaccggtttg
     2341 gtcatgttgg cggggtccat aatcttgtca agttgctctt ttgtgaggaa acctttttca
     2401 agaaccagct tgtatacgct gccaccggtt ttttgagcct cttgggcaac ctcggtgatt
     2461 ttggcgtagc caagataggg tttaagagca gtcacgatac cgatgctgta ttccacgagc
     2521 ttcttgcaat gttccttgtt ggcggtaata ccatccacac actctacgcg aagagtgtcg
     2581 caagctctca taagccaggt agcagactct acgagggact gtacaagtac aggctccatt
     2641 acgttgagtt caagctgtgc tgcctcggaa gccatggtaa tggctacatc gttaccgatt
     2701 atcttgaagc aaacctgatt tacaacctcc ggaataacgg gattgacttt accgggcata
     2761 attgagctac ccggctgctt gggaggaagg ttgatctctc caagacctgt acgggggccc
     2821 gaggagagca gacggaggtc attgcttatc ttggagatgc gaatagcaac attctttata
     2881 gcggaagaat agactacaaa agcggatgta tcattggatg cctcaatcag atcttctgcg
     2941 agggtcactt tccatccgga aatcttttta atatacttag tgcaggcctc tgcatagccg
     3001 ggctcggcag taatgccggt accgatagcc gtaccaccca tattgattac gagcaagccc
     3061 tgtgctgcct catccagacg tttca
//
LOCUS       DAOSIE010000174         3064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_27786_length_3064_cov_3.309737, whole genome shotgun sequence.
ACCESSION   DAOSIE010000174 DAOSIE010000000
VERSION     DAOSIE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27786_length_3064_cov_3.309737"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLI69_05475"
     CDS             complement(<1..256)
                     /locus_tag="PLI69_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG41971.1"
                     /translation="MKSYNTPLGELQIELFGHASVHFTINGKNWYLDPYCDVCSFEGK
                     TPADFILITHDHYDHYDEEALVHIVQEHTILVVPPVVPRKH"
     gene            complement(273..1523)
                     /gene="hutI"
                     /locus_tag="PLI69_05480"
     CDS             complement(273..1523)
                     /gene="hutI"
                     /locus_tag="PLI69_05480"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOG41972.1"
                     /translation="MSSLLIVNIGKLVQVREQTCSPLRGAQMDILPAIDDAFLFSEKG
                     KIAAFGSMSELPQSFPRSADKVIDAAGRMVLPAFCDCHTHLVYAGSREMEFIDKIRGL
                     SYQEIAARGGGILNSARLLHDTSEEELFRQAMVRVEEVMHFGTGAIEIKSGYGLTTED
                     EVKMLRVIRRIAEESPLTVKATFMGAHAFPERFRDDHEGYVSEIINEMIPAVAAEGLA
                     EYIDVFCEEGFFSREESDRILAAGRKYGLKATVHANQMSCSGGVEVGVKYDAESVSHL
                     EYAGDAQFEVLEKSETIANLLPGATFFLDMEYPDARRMLQHDIPVALATNYNPGSCPC
                     GDMKFIMALACMKMKMTPEEVINAATINSAFAMGLGETHGSITLGKVASLIITRPIPS
                     LEFIPYAFSTPLVENLILDEKIVI"
     gene            complement(1530..3014)
                     /gene="hutH"
                     /locus_tag="PLI69_05485"
     CDS             complement(1530..3014)
                     /gene="hutH"
                     /locus_tag="PLI69_05485"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOG41973.1"
                     /translation="MHTFYIDEKIHCLSDVMTLLEPDCRIELGHEAMERITRCRDYLD
                     RRLENQVEPIYGITTGFGSLCNVAVGAADLSKLQQNLVMSHACGTGDEVKEDIVRLML
                     ALKIKSLSYGYSGVKLETVQRLVDFFNHGVIPVVYSQGSLGASGDLAPLANMSLPLLG
                     MGDFDYRGERYSGEELETIFGWKPLKLASKEGLALLNGTQFMLAHALWSLCHAKRLSS
                     LADKIAALSFDAFDGRVEPFTPGVHEIRPHSGQITTAEMINIYLEGSEIISRPKEHVQ
                     DPYSFRCIPQVHGATKDALSHIEGVFITELNSATDNPTILPDEDLIISAGNFHGQPLA
                     INLDFLAIAMAELGSISERRTYQLISGKRGLPSFLIAHPGLNSGFMIPHYTAASIVSQ
                     NKQLCTPASVDTIDSSQGQEDHVSMGANAATKCVKVIQNVESLLAIELLNAAQALEFR
                     RPLRTSPILEEFVSEYRDIVPFIDEDEVMYHHIRRSILFLRNQK"
BASE COUNT          801 a          751 c          695 g          817 t
ORIGIN      
        1 aatgttttct gggcactaca gggggaacaa ccagtatcgt atgttcttgt acaatatgta
       61 cgagggcctc ctcgtcatag tggtcataat gatcgtgtgt gatcagaatg aagtctgcag
      121 gagtcttgcc ttcaaaagag cagacatcgc aatagggatc gaggtaccaa ttcttgccat
      181 tgatggtgaa atgtactgag gcgtggccga agagttcaat ctgcagctcg cccaatggtg
      241 tgttgtagct cttcataatg gtatctttat atttatatta caattttttc gtcaagtatc
      301 agattctcca ccagcggagt ggagaaagca taggggataa attccagcga aggtataggc
      361 ctggtgatta ttagggaagc caccttacca agggtgatgc tgccgtgcgt ctctcccagt
      421 cccattgcaa atgcactatt gattgtagcg gcattgatta cctcttcagg agtcattttc
      481 attttcatac aggccaaagc cataataaac ttcatatctc cacagggaca ggagccggga
      541 ttgtagttgg tggcaagcgc caccggaata tcatgctgca acattcttcg ggcatccgga
      601 tattccatat ccaggaaaaa agtagctccc ggcaagagat tggctatggt ctccgacttc
      661 tctaaaacct caaattgagc atcaccggca tactccaggt gagagaccga ttccgcgtca
      721 tatttcactc caacctcaac acctcccgaa caactcatct gattggcgtg tactgtagcc
      781 ttgaggccat attttctacc ggcagcaagt atcctgtcgc tttcctccct ggagaaaaat
      841 ccctcttcgc agaagacatc tatgtactcg gccaaacctt cggcagcaac tgccggtatc
      901 atctcattta ttatctcact gacatatccc tcatgatcat ctcgaaagcg ctcagggaag
      961 gcgtgtgccc ccataaaagt ggctttcacc gtaagcggag actcctcggc gatgcgcctt
     1021 attactctta gcattttaac ctcatcttcc gtagtgaggc cgtagccgct cttaatctct
     1081 atagcccctg taccaaaatg catgacctcc tctaccctca ccattgcctg ccgaaaaagt
     1141 tcttcctctg aggtgtcgtg aagcaaacgg gcagaattga gtataccgcc ccctcttgcc
     1201 gcaatttcct gataagatag gccgcgtatc ttatctatga actccatttc tctgctaccg
     1261 gcatatacca gatgggtatg acaatcgcag aaagccggaa ggaccatcct tcctgcagca
     1321 tcaattacct tgtcagcact tcggggaaaa ctctgaggaa gctcggacat agatccgaac
     1381 gcagctatct ttcctttctc agagaagaga aaagcatcgt caattgcagg cagaatatcc
     1441 atctgcgcac ctctgagtgg agagcaggtc tgctccctca cctgcacaag ctttcctata
     1501 ttgactatca gcagagatga catagatgat tatttttggt ttcttaaaaa taggattgag
     1561 cgtctgatat ggtggtacat tacctcatct tcatcgataa atggtacaat atcacgatat
     1621 tcagacacga actcttccag tatgggtgaa gtgcgtagcg gtctgcgaaa ctccaatgcc
     1681 tgggcagcat tgagcagttc tatcgcaagt agagactcaa cattttgtat caccttgaca
     1741 cactttgtag cagcattggc tcccatgctc acatgatctt cctgaccttg cgaggaatct
     1801 atggtatcta cagatgcggg agtacagagc tgcttgttct ggctcacaat ggatgcagct
     1861 gtataatgag gtatcataaa tccggagttg agaccgggat gcgcaataag gaaggaggga
     1921 agtcctcttt taccggagat cagctgatag gttctcctct ctgagatact gccaagctct
     1981 gccattgcta tcgcaagaaa atccagattg atagcgagcg gctgtccatg gaagttaccc
     2041 gccgaaatga ttaaatcttc atccggaagg atggtaggat tgtcggtagc cgagttaagt
     2101 tcggtgataa agactccctc aatatgcgag agcgcatctt tggtggctcc gtgtacttgt
     2161 ggaatacatc tgaaagaata ggggtcctga acatgctctt taggacgact aatgatctcg
     2221 ctcccttcca aatagatatt aatcatctcc gcagtggtga tctgtccgga gtggggtctt
     2281 atctcatgca cgcccggggt aaatggctca accctaccat caaaggcatc gaaggagagc
     2341 gcggcaatct tgtcagccag cgaagagagt ctcttcgcat gacagagcga ccaaagtgcg
     2401 tgtgccagca tgaactgtgt tccgttgagt aaagctaatc cctctttaga tgcgagcttg
     2461 agaggcttcc agccaaatat ggtttccagt tcttctccgg aatatctctc tccgcgataa
     2521 tcaaagtctc ccataccgag caagggtagg gacatgtttg caagtggagc gagatcacct
     2581 gatgctccga gtgagccctg gctataaacc acaggtatta ctccatgatt gaaaaagtcc
     2641 acaagacgtt gtacagtctc cagtttaacc cccgaatatc cgtaggatag ggatttaatc
     2701 tttaaagcga gcattaagcg cacaatatct tctttcactt cgtcacctgt accacaggca
     2761 tgcgacataa ctagattttg ctgaagttta gagagatctg cggcccctac ggccacgttg
     2821 catagcgacc cgaagcctgt agtgatgccg tatatcggct caacctgatt ttccaacctc
     2881 ctgtcaaggt aatcacggca ccttgtaatg cgttccatag cctcatgtcc gagttcaatg
     2941 cggcagtccg gttccagcaa tgtcatcaca tccgagaggc agtggatttt ctcatctata
     3001 taaaaggtat gcatatatgt tagaatttgc tttagttttc tcttcgcaaa ttacaattat
     3061 aaga
//
LOCUS       DAOSIE010000175         3060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_27826_length_3060_cov_4.091514, whole genome shotgun sequence.
ACCESSION   DAOSIE010000175 DAOSIE010000000
VERSION     DAOSIE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27826_length_3060_cov_4.091514"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="PLI69_05490"
     CDS             complement(<1..1626)
                     /locus_tag="PLI69_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOG41974.1"
                     /translation="MITIYCVNTGTRQEFPEGTTLQNMVEAFDFERPHPIIAAKVNNV
                     SQGLRYRAFNNRNVEFLDYTSYTGRGVYRRSLCFLLYKAARDIFPECRVVIRRPISKG
                     FFCYIDKDGKGSTTLSKEELDKLRARMQELVDQDIPFRRHEVQKEEAMEVFRSVGAED
                     KVRLLETAGDVYVIYYTLLETPDYYYDALLTGTGYLKVWSLTPYKNGLLLRVPDRHNP
                     QQLTALVEQPKTFEIFSEANRWNHIMGLSTVGDLNAAIARGKSSDLINVAEALQEKKI
                     VQIAEEIDGRMKSDKPLRLILITGPSSSGKSTFCKRLSIQLMACGLKPVSFSTDDYFV
                     NRLDTPRLPDGSFDFDNFETVDHKYLQSDMLKLLNGETVEVPEYNFVTGMREFNGRRL
                     HLEEGSILIVEGIHALNPRLTDRVEESLKYRIFINTITSIALDDHNCIPTSDNRLLRR
                     IVRDYNLGSFTAMETISNWPNVRRAEVKWIYPFQEDADVMFNSSYLLEFAVLHSHAER
                     ILRTIPKNCKEYSEAHRLLKFLNYFTPVSDKDVP"
     gene            complement(1630..2088)
                     /locus_tag="PLI69_05495"
     CDS             complement(1630..2088)
                     /locus_tag="PLI69_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOG41975.1"
                     /translation="MTLSLPLIIAYSSMAIMLAFACIGSAIGVTMAGNTTIGSLKKNP
                     DIFGKAMILCALPSTQGLYGFAGFFFMLNAIKGLNFEITLGASLAMAAAAVALGFTAY
                     FSAIWQAKLCANGVNEMGNGHDVFSRTMILAVFPELYAILTFAATFLALP"
     gene            complement(2119..>3060)
                     /locus_tag="PLI69_05500"
     CDS             complement(2119..>3060)
                     /locus_tag="PLI69_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41976.1"
                     /translation="PIKLHNNRFSRLFELIGNLYMLPTYDEVDLTPYFAPFYMLFFGL
                     CLGDMGYGLIILLASLFVIWKVPSLREYGKLGLWLGIGTIIMPMLNGTFFGAKIYDLF
                     NMSDNIKGLFFTDIKMFWFAIIFGLFQIIVARLISAIYKMRRIGWQHGMSNIGWCMIL
                     IWIALLYAGSQMGRELMSPWVGRILGIGGLALVLLFTKPVRNPFKRLFGGVGALYDIT
                     GFFGDVLSYIRLFGLGAAGGSLGMVINAMSMALKGVPYAGWILFIVLLIFGHLFVMLL
                     SAIGAFVHPMRLTFVEFYKNADFTGGGKPYRPLNKNN"
BASE COUNT          834 a          683 c          740 g          803 t
ORIGIN      
        1 ggggacatct ttgtctgaga caggtgtgaa ataattaagg aatttaagaa ggcgatgagc
       61 ctcgctatat tccttgcagt ttttgggtat tgtcctgagg attctctccg catgagaatg
      121 aagcacggca aattcaagca gataggagga gttgaacatc acatcggcat cctcctggaa
      181 ggggtagatc catttcacct cggctcgcct tacattaggc cagtttgaga tagtttccat
      241 tgcggtaaag gaacctaaat tgtagtcgcg cacaatcctg cgaaggagtc ggttgtcact
      301 ggtggggata cagttgtggt catccaatgc tatgctggtg atggtattta tgaaaatcct
      361 gtacttgagg ctctcttcca cccggtcggt caggcggggg ttgagcgcat ggataccctc
      421 cacaatcagg atggaaccct cttcaagatg gagtctgcgt ccattaaact cgcgcattcc
      481 tgtgacgaaa ttatattccg gtacctcaac ggtttctccg tttagaagtt tgagcatgtc
      541 gctctggaga tatttatggt cgacggtctc gaaattatca aaatcaaacg agccgtcagg
      601 cagtcgtggt gtatccaggc gattgacgaa gtagtcatcg gtggagaatg aaactggctt
      661 tagtccgcag gccatgagct gtatgctcaa acgcttgcag aaagtggact ttccactgct
      721 gctggggcct gtgattagaa taagacgcag aggcttatcg gatttcatcc tgccgtcaat
      781 ttcttctgct atctgtacga ttttcttctc ctgaagagct tcagccacat tgataaggtc
      841 tgaactctta ccacgtgcaa tggctgcatt taggtctccc accgtactga gtcccattat
      901 gtgattccag cggttggcct ccgagaatat ctcgaatgtc tttggctgtt ctaccaaagc
      961 tgtcaattgt tggggattat gcctgtcagg tactctgagc agcaatccat ttttgtaagg
     1021 ggtcagactc cataccttga ggtatccggt gccggtaagc agcgcatcgt aatagtagtc
     1081 gggagtttct aaaagggtgt aatatatcac atacacatct cctgccgttt ccagcagtcg
     1141 taccttgtct tctgcgccta cgcttcgaaa aacttccatt gcttcctctt tctgcacttc
     1201 atgccggcgg aaagggatat cctgatccac caattcttgc attcttgcac gcagtttatc
     1261 cagttcttct ttagagaggg ttgtggaacc ttttccatct ttatcgatat agcagaagaa
     1321 gccctttgat atgggtctcc gaatgaccac ccggcattcc ggaaaaatat cgcgtgcagc
     1381 tttgtaaagc aggaagcaga gggacctgcg atatacgccg cgaccggtat aggaggtgta
     1441 gtccaaaaac tccacgttgc ggttgttgaa agcgcggtag cggagtcctt gacttacatt
     1501 gttgaccttt gcggctataa taggatgagg cctttcgaaa tcgaaggcct caaccatatt
     1561 ttgaagggtt gtcccttcgg gaaattcttg cctggtccct gtattgacgc agtagatggt
     1621 aatcattgct tacggcaagg cgaggaatgt agcggcaaat gtgagaatgg cgtaaagctc
     1681 agggaatacg gcgagaatca ttgtcctgga gaaaacatca tgtccgttac ccatctcgtt
     1741 aacaccgttt gcacagagct tagcctgcca gatggccgag aagtaagctg taaaacctaa
     1801 tgctacagca gctgctgcca ttgcgagtga agcgcctagt gtgatctcaa aattgagtcc
     1861 cttgatggca ttcagcatga agaaaaatcc tgcaaatccg tatagaccct gggtcgaagg
     1921 caatgcacaa aggatcattg ctttaccgaa aatatcggga tttttcttaa gtgaaccgat
     1981 agttgtgttt ccagccatgg ttacaccgat agcactgcct atacaggcaa atgccagcat
     2041 tattgccata cttgagtaag cgatgataag tggtagtgat aaagtcatat tgattgaaat
     2101 tttaagtttg aaatcggatt aattgttttt atttagagga cggtaaggtt tgccgccgcc
     2161 tgtaaaatcg gcatttttat aaaattctac gaaagtcaga cgcatggggt gcacaaatgc
     2221 accaatagcc gagagtagca ttacgaaaag gtgaccgaag atcaaaagta caatgaaaag
     2281 aatccatccg gcgtatggga cacccttgag ggccatagac atggcattaa ttaccatacc
     2341 aaggctgcct ccggctgcac ctagtccgaa cagacggata taggagagta catctccaaa
     2401 gaacccggtg atgtcatata aggccccaac tccgccgaaa aggcgtttga aagggttgcg
     2461 taccggtttg gtaaagagca gcaccaaagc aaggccacct attcccaaaa tccgtccgac
     2521 ccagggagac atcagctctc tccccatctg cgaacccgca tatagcagcg caatccatat
     2581 taatatcatg caccaaccga tgttggacat gccatgttgc catcctattc tgcgcatctt
     2641 gtaaattgca gaaatcagtc gtgctacgat aatctggaat aggccgaata taatggcaaa
     2701 ccagaacatc ttgatatcgg tgaagaaaag tccctttatg ttgtcagaca tattaaacaa
     2761 gtcataaatc tttgcaccga aaaacgtacc gttgagcatc ggcataatta tggtacctat
     2821 acccagccaa agtccaagtt tgccatattc tcgcagggaa ggaactttcc agatgacaaa
     2881 caatgaagcc agtaggatta tcagaccata tcccatatct cccagacaga gtccgaagaa
     2941 aagcatatag aagggtgcaa aatagggtgt gaggtcgact tcatcatatg tgggcagcat
     3001 gtatagattg ccgataagct caaatagtct ggagaagcga ttgttatgga gtttgatagg
//
LOCUS       DAOSIE010000176         3059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_27838_length_3059_cov_3.627497, whole genome shotgun sequence.
ACCESSION   DAOSIE010000176 DAOSIE010000000
VERSION     DAOSIE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27838_length_3059_cov_3.627497"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PLI69_05505"
     CDS             complement(<1..997)
                     /locus_tag="PLI69_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007896973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HOG41977.1"
                     /translation="MIRKVKFESQNRRIDKINKVLKSYGIASIEEANKICDEYGIDPY
                     LECEQTQKICFENAKWAYVVGAAIAIKKGCKNAADAAEAIGEGLQAFCIAGSVADDRK
                     VGLGHGNLAARLLRDETKCFAFLAGHESFAAAEGAIKIAEMANKVRKQPLRVILNGLG
                     KDAAMIISRINGFTYVQTEFDYYTGKLKEVKRKSYSNGPRAKVNCYGADDVREGVAIM
                     HKEGVDVSITGNSTNPTRFQHPVAGTYKKECVQQGKNYFSVASGGGTGRTLHPDNMAA
                     GPASYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGFLGMGNNPMVGATVAVSVSIEQ"
     gene            complement(1019..1720)
                     /locus_tag="PLI69_05510"
     CDS             complement(1019..1720)
                     /locus_tag="PLI69_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41978.1"
                     /translation="MIYSHEVEHMCVVKKGPNHGPSPIPGEGKWVKVKEVKDISGFTH
                     GIGWCAPQQGACKLTLNVKKGIIEEALIETIGCSGMTHSAAMAGEILPGKTLLEALNT
                     DLVCDAINTAMRELFLQIVYGRTQSAFSEGGLIVGACLEDLGKGLRSTVGTMFSTKAK
                     GVRYLETAEGYVSKIALDEDDEVCGYEFVRLGEMMDLIKKDMDANEALKKATAHYGRF
                     TAEQGAVKVIDPRID"
     gene            complement(1877..1949)
                     /locus_tag="PLI69_05515"
     tRNA            complement(1877..1949)
                     /locus_tag="PLI69_05515"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1914..1916),aa:Gly,seq:ccc)
     gene            2073..>3059
                     /gene="lysA"
                     /locus_tag="PLI69_05520"
     CDS             2073..>3059
                     /gene="lysA"
                     /locus_tag="PLI69_05520"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOG41979.1"
                     /translation="MITKQAIEVFKKIETPFYYYDVELLRRTLDIYKSQLEKYGYHAH
                     YALKANANDRILDIIKGYGLGADCVSGNEVKLAVKAGFPADKIVFAGVGKSDKEIKAA
                     LDADIFGFNCESIPEISVINELAAKMGKKARVSLRVNPDIDAHTHKYISTGRKEDKFG
                     ISPWMFQDVADMMAKCKNIDFIGLHFHVGSQITDLAVFKLTCKRVEELQKWFIDRGFF
                     IKNINLGGGLGIDYNNPNENPIAPLKEYFKIINENLIVRPGQDVHFEPGRAIVGQCGH
                     LISRVLYVKIGQRKKFAILDAGMNDLIRPALYQAHHAIDNLTSTGRLLRYDVV"
BASE COUNT          815 a          713 c          650 g          881 t
ORIGIN      
        1 cttgttctat agatacggaa actgccacag tggcgccgac catcggattg ttacccatgc
       61 cgaggaatcc catcatctcc acatgtgcag gaacgctgga cgaacctgca aactgtgcat
      121 cggagtgcat acgtcccata gtgtcggtca taccgtagga cgcggggccg gctgccatgt
      181 tatcgggatg gagcgtacgt ccggtaccac cacctgatgc cacagaaaaa tagttcttac
      241 cctgctggac acactctttc ttataagtgc cggccacagg atgctgaaaa cgtgtaggat
      301 tggtggaatt gcctgttatt gaaacatcaa caccttcttt gtgcatgatg gcaactccct
      361 ctcgaacatc atccgcacca tagcagttga cttttgcacg tggtccgttg gagtaggact
      421 ttctcttcac ctccttaagt tttccggtat aatagtcaaa ttcagtctga acataggtaa
      481 agccgttaat tctggaaatg atcatggcgg catctttgcc tagtccgttg agaattacac
      541 gaagaggctg cttacgtact ttgttggcca tttcagcaat cttaatggct ccttctgcag
      601 ccgcgaatga ctcatgaccg gctagaaaag cgaagcactt agtttcatca cggaggaggc
      661 gcgcagctag attgccgtga cccagaccaa ccttacggtc gtctgctacc gaacctgcta
      721 tgcagaatgc ctgaagaccc tcgccaatag cttcggcggc atcagctgca tttttgcaac
      781 ctttcttgat tgcgatagct gcacctacga cataggccca ttttgcattc tcaaagcata
      841 tcttttgtgt ctgctcgcac tccaggtagg ggtcaattcc atactcatca caaatcttgt
      901 ttgcctcttc gatggaggcg atgccataag atttcaacac tttgttgatc ttgtcaatac
      961 gacggttttg gctttcaaat tttactttac gtatcattgt ttaatatacc tcctcttttt
     1021 aatcgattct gggatcaata accttaactg cgccttgctc ggcagtaaat ctcccatagt
     1081 gtgcagtagc cttttttagt gcctcgtttg cgtccatatc tttcttaata aggtccatca
     1141 tttcacctag ccgtacaaat tcataaccgc atacctcgtc atcttcatca agtgcaatct
     1201 tactaacata accctcggca gtttcgaggt aacgaactcc tttagctttg gtcgagaaca
     1261 tggtaccgac cgtggagcgt agtccctttc cgagatcttc cagacacgca cctactatga
     1321 ggccgccctc cgagaaagcg gattgggtac gtccatagac aatctgaagg aagagttcac
     1381 gcatagctgt gtttattgca tcgcacacca ggtcggtgtt gagagcctcc aaaagagttt
     1441 tgcccgggag aatctcaccg gccatggcag cggaatgtgt cattccggag cagcctattg
     1501 tctctataag agcctcttct ataatgccct tcttgacatt gagggtaagc ttacaggcac
     1561 cttgctgagg tgcacaccag ccaatcccgt gcgtgaaacc cgatatatct tttacctctt
     1621 ttactttcac ccatttgccc tctccgggaa taggtgaagg tccgtggtta ggtcccttct
     1681 tgacaacaca catgtgttct acttcgtgtg aataaatcat aatttatgtc tgatatttaa
     1741 tttaaaactt tctcttcttg ttcaggtata aaaataattt gacaaaattc gtatgcattt
     1801 ttaaataata caaatcgttt ttaataattc gaaaggcctt acttaggttg ccaacaacga
     1861 ttttttagat catcggagcg gaatacgggg ttcgaacccg cgaccttcgg cttgggaagc
     1921 caacactctg ccaactgagc taattccgcg aaattatcat acaaatataa gaaaaatcgt
     1981 gagaatatac aaatcacctt caaaaaagat catttcttga tattatgtca ttttttcgta
     2041 cttttgcaag attaataaca aagtactcca cgatgattac caaacaggca attgaagttt
     2101 ttaaaaagat tgaaactccc ttctattatt atgatgtaga gcttctcaga aggactctcg
     2161 atatctataa atcccagctc gaaaaatatg gttatcatgc tcattatgct ctgaaggcca
     2221 atgccaatga tcgcattctt gatataatca aagggtacgg tctcggtgca gattgtgtga
     2281 gtggaaatga ggttaaactt gcagttaagg caggtttccc tgctgataaa atagtttttg
     2341 caggtgtggg taaatcggat aaggagatca aagcggcact tgatgccgat atttttggat
     2401 tcaattgcga gtcaattccg gaaattagtg taatcaacga acttgcggcg aagatgggga
     2461 aaaaggcgag ggtctcactt cgtgtcaatc ccgatataga tgcacacacc cataaatata
     2521 tcagtaccgg ccgtaaagag gataaattcg gtatcagccc ctggatgttt caggatgttg
     2581 ccgatatgat ggcaaagtgc aagaatatcg acttcatcgg ccttcatttt catgttggct
     2641 cccagataac cgatctggcc gtcttcaaac ttacctgtaa gagggtagag gagttgcaga
     2701 aatggtttat cgacagaggt tttttcatta aaaacatcaa cctcggtggt ggcctcggca
     2761 tcgattataa caatcccaac gaaaatccca ttgcaccatt aaaggagtat tttaagatta
     2821 taaatgaaaa cctcatagtg cgtcccggac aggatgtcca ttttgaaccg ggcagggcca
     2881 ttgttggtca gtgtggtcat cttatctcca gagtactcta tgtaaagatt ggtcagcgta
     2941 aaaaatttgc tattctcgat gccggtatga atgaccttat ccgtcctgcc ctttaccagg
     3001 cgcatcatgc aatagataat cttacctcca caggccgcct cctcaggtac gatgttgtt
//
LOCUS       DAOSIE010000177         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_27890_length_3056_cov_3.909697, whole genome shotgun sequence.
ACCESSION   DAOSIE010000177 DAOSIE010000000
VERSION     DAOSIE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27890_length_3056_cov_3.909697"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="pepT"
                     /locus_tag="PLI69_05525"
     CDS             complement(<1..815)
                     /gene="pepT"
                     /locus_tag="PLI69_05525"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOG41980.1"
                     /translation="MESVKEKFLRYISFDTQSDPESDSQPSTAKQLALSMQLVKELHD
                     MGITDASLDKDGYVMATIPSNIERDVPAIGFIAHVDTAPDASGENIKPQIVKNYDGGN
                     ILLNKEMDLYLRVKDFPEMKQYKGQKLITTDGTTLLGADDKAGVAEIMCAAEYIMAHP
                     EIPHGPIKIGFTPDEEIGRGVDHFDVEKFGAKYAYTLDGGEIGELEYENFNAASAKIF
                     IQGRNIHPGYAKNKMVNAMLIAMELNAMLPVQQRPEFTEHYEGFFHIIGFNGTV"
     gene            complement(817..2796)
                     /locus_tag="PLI69_05530"
     CDS             complement(817..2796)
                     /locus_tag="PLI69_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014710343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOG41981.1"
                     /translation="MENKENKPQGLPENAYRKLKKGEEYKPLLSPDKNYPEITVWSVT
                     IGLVMTIIFSAAAAYLGLKVGQVFEAAIPIAILAVGLSSVLKKNQALGQNVIIQSIGS
                     CSGVIVAGSIFTLPAIYILGLDVNFTQMFLSSLLGGVLGILFLIPFRKYFVKEQHGHY
                     PFPEATATTQVLVSGERGGKSLKVLLIAGLIGGLYDFIVATFGAWAETVTTTVTRWGA
                     AFADKTKIVLKLNTGAAVLGLGYIVGLKYAFIICCGSLLVWLIINPILAAAFSGVNIF
                     GMSFSAALGQMSADEIFRTYSRLIGIGGIATAGIIGIIRSSKVISSAIKLAIGEFSKK
                     DSGEKVQVKRTDQDISMRSVVLGIAVALLGVFAFFAFGGVVHTIWQALVGMLVVAVIA
                     FLFTTVAANAIAIVGTNPVSGMTIMTLIITALVLVATGLKGNAGMVASLLIGGVVCTA
                     LSTAGGFITDLKIGYWIGTTPAKQEKWKFLGVLVAAVTVAGVVIVLNKTYGFVGEGAL
                     VAPQANAMAAVIKPMMEGGSAPWFLYGIGAAIAIILTFLKVPALAFCLGMFIPIDLNL
                     PLLLGGAIAWFVSTRSADKEVNAARKERGTLIASGFIAGGALMGVVSAILKFAKVDWF
                     FTDWNATYGEAIAIIPLIALVIYMIMASMKVEKQQ"
BASE COUNT          797 a          735 c          704 g          820 t
ORIGIN      
        1 accgttccgt tgaatccgat gatatggaag aatccctcgt aatgctcggt aaattccggt
       61 ctctgttgca ccggcagcat ggcattgagt tccattgcga tgagcattgc attcaccatc
      121 ttgtttttgg catagccggg atggatatta cggccctgaa tgaatatttt ggctgatgcg
      181 gcattgaagt tctcatactc gagctctcct atctctccac cgtccaatgt ataggcgtat
      241 tttgcaccaa atttttctac atcaaagtgg tcaacgcccc gacctatctc ctcatcggga
      301 gtgaaaccga tctttatggg gccgtgtggt atttccgggt gcgccattat atactcagca
      361 gcgcacatta tctctgcaac acccgcctta tcatcagctc ccagaagcgt tgtgccatct
      421 gtagtaatta gtttctgccc tttgtattgc ttcatttcgg gaaaatcctt tacccggaga
      481 tagagatcca tctctttgtt tagcagaatg tttcctccgt cataattttt cacaatttgt
      541 ggtttgatat tctctcctga tgcgtcgggt gcggtgtcca catgcgcgat aaagccaatc
      601 gccggaacat ctctctctat attagaaggt attgtggcca ttacatagcc atccttgtcc
      661 agagaggcat ctgttatccc catatcgtgg agttccttca ccagctgcat ggagagcgca
      721 agctgttttg cagttgacgg ctggctatcc gactcgggat cagactgggt atcgaatgat
      781 atatatctta gaaatttttc ctttacactt tccatattat tgctgttttt caactttcat
      841 agatgccatt atcatatata taacaagtgc tataagtggt atgatggcga tggcttcgcc
      901 ataggttgca ttccagtcgg taaagaacca atcaactttc gcaaacttta gaattgcact
      961 aacaactccc attagagcac caccggcaat aaatccggaa gcgataagag tacctctctc
     1021 cttgcgggca gcatttacct ccttatccgc gcttcgtgtg ctgacaaacc aagcgatagc
     1081 tccaccgagg agtaacggca ggttgagatc aatgggaatg aacatgccga ggcagaatgc
     1141 aagtgccgga actttcagga aggtgaggat aatagcaatg gctgctccta ttccgtagag
     1201 gaaccatggg gcgctgcctc cctccatcat gggtttgatt acagctgcca tagcattggc
     1261 ttgaggggct acgagtgccc cttcgcccac aaatccgtag gttttgttga gcactatcac
     1321 cactccagct acggttacgg cagccaccag cacgccgagg aatttccatt tttcctgctt
     1381 cgctggggtg gtaccaatcc agtatccgat cttgaggtcg gtgataaagc cgccggcggt
     1441 ggaaagcgct gtgcagacca ctcctccgat cagtagggag gcgaccatgc ctgcattgcc
     1501 cttaagtccc gtggctacaa ggaccagtgc tgtgatgatc agtgtcataa tggtcatacc
     1561 gctgaccgga ttagttccta cgatagcaat ggcgttggcc gctactgtag tgaagaggaa
     1621 ggcgatgact gctactacga gcattccgac aagcgcctgc cagatagtgt ggacaactcc
     1681 gccaaaggca aagaatgcga agactccgag cagagctacc gctatgccca gaaccacact
     1741 tctcatagag atatcctgat ctgtgcgctt aacctgtact ttctccccgc tatccttttt
     1801 gctaaactcc cctatggcga gcttgattgc ggagctgata acttttgaag atctgatgat
     1861 acctatgata cctgccgtgg caatgcctcc gataccaatc aaacgagagt aggtcctgaa
     1921 tatctcatca gcggacatct gtccgagggc cgcagagaaa ctcataccga aaatattcac
     1981 tccggagaaa gcggcggcca gtatagggtt gatgatcagc catacgagca acgagccgca
     2041 acagatgata aaggcatatt tgagtcctac aatataaccc aaaccgagta ccgcagcccc
     2101 ggtattgagt tttagcacta ttttggtctt gtccgcaaat gccgcacccc aacgggtaac
     2161 cgttgtagtg acggtctcag cccaagctcc aaaggtagct actatgaaat cgtaaagtcc
     2221 accaatcaga cctgcaatga gcagaacctt gagactcttt ccgccgcgct caccgctaac
     2281 caatacctgt gtggtggcag tagcttcagg gaagggatag tgtccgtgct gttcttttac
     2341 aaaatacttc ctgaagggaa taaggaaaag aatacccagt acacctccga ggagagaact
     2401 caggaacatc tgggtaaaat tgacatccag accaagaata tagattgcag gtaatgtaaa
     2461 tattgagccg gccactatta ctccggaaca ggatccgatg gattggatga tcacattctg
     2521 ccctagggcc tgatttttct taagaacgct tgagaggcct acggccagaa tggcaatcgg
     2581 gatagctgcc tcaaagacct gtcccacctt aaggccaagg tatgcggctg cggctgaaaa
     2641 gatgatggtc attaccaatc ctatagtcac ggaccagact gtaatttcgg ggtaattctt
     2701 gtccggtgat agcaatggct tatattcttc ccctttttta agtttgcgat atgcattctc
     2761 aggtagccct tgtggcttgt tttccttgtt ttccataagt agtatagagt tatctttagt
     2821 tatttttaca aaaactcaac aaaactacga aataatttgg agattggaaa aaagtgttta
     2881 cctttgctgc ccgtttcggc gagaaacgca tgttcattga cattttgaga tagataacga
     2941 ggtaaaaaaa ctgtaaaaag ataagatagt ctgtaatttt aagtaaagga gtttttcttt
     3001 cataattacg ggactacaat ttcaagaggc agtgcgtaga cactgcaagt gataga
//
LOCUS       DAOSIE010000178         3053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_27935_length_3053_cov_3.322548, whole genome shotgun sequence.
ACCESSION   DAOSIE010000178 DAOSIE010000000
VERSION     DAOSIE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27935_length_3053_cov_3.322548"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLI69_05535"
     CDS             complement(<1..762)
                     /locus_tag="PLI69_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOG41982.1"
                     /translation="MIPEFAIREWSETVSWIESEQVEQDLLICRTLTEIYKDEYLASH
                     LAFRGGTALHKLYLSPQSRYSEDIDLVQVKAEPIKETYDRIREVLSFLGEPKVRQKRN
                     NNTLIFRMESEMPPVVPIHLKVEINCKEHFNVLPMQEIPFSVTNKWYSGECNVLTYQL
                     EELIGTKLRALYQRSKGRDLYDLYKALTIREINIEKVLNCYNAYMDFVVDHTPTYKEF
                     IINLENKMQDDEFLGDTRQLLRSDEDFSPQEGYKVV"
     gene            complement(759..1574)
                     /locus_tag="PLI69_05540"
     CDS             complement(759..1574)
                     /locus_tag="PLI69_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HOG41983.1"
                     /translation="MKQQEQSISDWINEKLLRGYYTFTIEDIQNNFPQFSKAYISTSL
                     YRLTVKKKIISPWRGFYVIMPIEFALKGIIPPIFYIDALMAFLNKKYYVCLLNAASFY
                     GASHQRVQRFSVMTEAPKLRNTAKTGTPILFFSKKDIPEKFIRHHKTQLGYVNVSSPE
                     LTAIDLVANEKYVGGLNRVCTVLNELVESFDFDKLEDDFFDLAPTPIYQRFGYILDSV
                     LEREDLAEIFKTKISLNLTPIRNVPFKVGKSTEGCIIDKKWKVIINQEIDIDE"
     gene            1952..2881
                     /locus_tag="PLI69_05545"
     CDS             1952..2881
                     /locus_tag="PLI69_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG41984.1"
                     /translation="MGYTGELISLAVAFSWTATALFAEVGTKRIGSLQLNIIRMTLSL
                     LMLGATLWIVTGAPWPRFADGQTWFWLLLSGLIGYVFGDWCLFNSYLVIGARFGQLFM
                     TLAPPVAAITGWALLGETISLKGLLGMVVTLSGIAMSILSRGQGHKVAVKLPIKGVLL
                     GIGAGVGQGVGLVLSKVGMGHYHSAIPEGATSVSTMVPFASTFIRAVMGAIGFILILL
                     IRKEGPKLVHALNDRRGMLYASLATITGPFIGVSLSLMAVQYTNAGIASTIMALTPVL
                     IIIPSYLIFKQKVKVIEVIGAIISIAGVSLFFI"
BASE COUNT          856 a          652 c          563 g          982 t
ORIGIN      
        1 tacaacttta tatccctctt gtggactgaa atcttcatca gaacgaagca attgccgggt
       61 atcacccaag aattcatcgt cttgcatttt gttctcaaga ttaattataa actccttgta
      121 tgtgggggta tggtcaacca cgaagtccat atacgcatta tagcaattta agaccttctc
      181 tatatttatc tctcgaatag taagtgcctt atataaatca taaagatctc ttcctttact
      241 tcgctggtat aatgcccgaa gtttggtccc aataagttcc tcaagttgat atgtaagaac
      301 attgcattcc ccactatacc acttgttagt tacagaaaag ggaatttctt gcataggcaa
      361 gacattgaaa tgctccttgc agttgatctc tacttttaaa tgtatcggca caaccggagg
      421 catctcagat tccatcctga atattagcgt attattattc cttttttgcc ttaccttagg
      481 ttccccaaga aacgacaata cctctctgat acggtcatag gtttctttga ttggctccgc
      541 tttaacctgt acgaggtcaa tatcctcact atatcgggac tgaggtgaaa gatataactt
      601 atgtaatgcc gtaccgccac gaaaagcaag atgcgaggcc aaatattcat ccttatatat
      661 ttcagtcaag gtcctgcaaa taagcaaatc ctgctccacc tgttctgatt ctatccaaga
      721 aacagtttca ctccactctc ttattgcaaa ttccggtatc attcgtctat atctatttct
      781 tggttaatta ttactttcca ttttttatct attatacagc cttctgttga tttacctact
      841 ttaaagggca catttctaat tggagttaga ttaagagaaa ttttggtctt aaatatttct
      901 gcaaggtctt ctctttccaa aacagaatct aaaatatacc caaatctttg atatatagga
      961 gttggtgcaa gatcaaaaaa atcatcttcc agtttatcaa aatcaaagct ctctaccaat
     1021 tcatttagca cggtacatac cctattcaat ccaccgacat acttttcatt cgccaccaga
     1081 tcaattgcgg ttaattccgg agatgataca tttacataac ccaattgggt cttatgatgc
     1141 ctgatgaatt tttccggtat atctttcttt gagaagaaaa gaatgggcgt tcctgtttta
     1201 gcggtatttc taagttttgg tgcttccgtc attacggaaa atctttgtac cctctggtga
     1261 gaagccccat aaaaagaagc cgcgttaagc aagcatacat aatatttttt atttaggaat
     1321 gccattaacg catcaatata aaatatcgga ggtataatcc ctttcaaagc aaattcaatt
     1381 ggcataatta cataaaatcc cctccaagga gaaataattt tcttcttgac ggttagacga
     1441 tataatgagg tacttatata ggccttacta aattgaggaa aattattttg aatatcctca
     1501 atagtgaaag tataataacc tctcaataac ttttcattaa tccaatctga tatactttgt
     1561 tcttgctgtt tcataattat tgcaatctgg ttgcaaatat aatcatttta tgatgaatat
     1621 tgcaactctg tagcaaatat ttaataactc tcataaaatg attaagtgct acttaatatt
     1681 ttattcttac agaattgatt gacatttcca ttatttcgga gtttaaatgt attgattatt
     1741 catagaacaa aagaatgctg cagtagatta actacatctt ttttgtatct ttgatatatc
     1801 gcaaacagcg atgccataaa cataatgaaa aagctccttg ttgtgaaaac attaggttcc
     1861 ctcgtccttt aacgggggat ttttttatcc caactcgatt ctcctaactc gatactccca
     1921 actccaaaaa taactatctt tgccgcctgt tatgggatat acaggagaac tcatatcatt
     1981 ggcggtagca tttagttgga cggctacagc gctttttgcc gaagttggca caaaacgcat
     2041 agggtcgctg cagctaaata tcataaggat gacactgtcg ctactgatgc tcggtgcaac
     2101 cctttggata gtgaccggag caccctggcc gcgctttgct gatggccaga cctggttttg
     2161 gctgcttctt tcgggactca tagggtatgt gtttggcgac tggtgcctct tcaactccta
     2221 cctggtaatt ggtgcgcgtt tcgggcagct ctttatgact ctggctcctc cggtggcagc
     2281 tatcacgggc tgggcactac ttggggagac tatctcattg aaaggattat tgggtatggt
     2341 agttactctc tcaggaattg ccatgtccat tctaagtaga ggtcaggggc acaaagtggc
     2401 cgtcaaactt cctataaagg gagttttgct gggcataggc gcaggagttg gccaaggagt
     2461 ggggctggta ttgagcaagg taggtatggg ccactatcat tctgcaatcc ctgaaggtgc
     2521 tacttcagtt agcacaatgg ttccctttgc gtcgaccttc attagagcgg tgatgggtgc
     2581 tatcggattt atattaatcc ttctgattag aaaagaaggt ccaaagcttg tccatgccct
     2641 aaatgatcgc cggggaatgc tttatgcctc tctcgctacc attacgggac cctttatcgg
     2701 tgtctcgctc tcactgatgg cggttcaata cactaacgcc ggcatagcat cgactattat
     2761 ggcactaaca ccggttctta ttattattcc ctcttacctg atttttaagc aaaaagtgaa
     2821 agttatagag gtcattggcg ccatcatcag tatcgcagga gtgtcgctgt tttttatata
     2881 ggttaggaaa agatatttgt caataacttt tggcagtata aggtcttcat tcgccaacac
     2941 cactactcct tccctcccct tcaaaacctc gctgtaactc ataatcaagt cccgcaacac
     3001 gtaccacgaa acacaaaacg cagaactcca cgccttttag cgtaatgttc tgc
//
LOCUS       DAOSIE010000179         3040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_28103_length_3040_cov_3.973199, whole genome shotgun sequence.
ACCESSION   DAOSIE010000179 DAOSIE010000000
VERSION     DAOSIE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28103_length_3040_cov_3.973199"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLI69_05550"
     CDS             <1..240
                     /locus_tag="PLI69_05550"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HOG41985.1"
                     /translation="RYKSGIFYHPGYTSDADIAQKVYEEVSKEYSVPIYVEIGPVTSY
                     YPAEEYHQNYLDKNPGGYCHIKPQLYEFAKIVNRK"
     gene            complement(250..1419)
                     /locus_tag="PLI69_05555"
     CDS             complement(250..1419)
                     /locus_tag="PLI69_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HOG41986.1"
                     /translation="MGAKPIEGVLALHSEMKRWMQYIHSHPEVSTKEHETARYIAKEL
                     AQMGYEVHEKIGKDGIYGVVGRLKNGDGEKSIGFRADMDALRIQEDNNLSYKSRYDGI
                     SHLCGHDGHSAMALGAAKYLAETRRFNGTVHFIFQPSEENMQGGPAMIGGGLFEKFNI
                     DRVYAMHNIPGLPKGVLHFHDGETMSAVDNWEIELFGKGSHGSMPELSIDPVVAGASL
                     VMALQTIVSRNISPWDNSVVTIGAFLAGNEGNVIANGAILRLSMRNMQAETRKKVLDR
                     IRKITAAQAESYGCTYEIREGQPGAVLVNSAEETKFAADVAKHYFGDENVVYPCKPLM
                     SSEDFAFMLQQKPGNYIMIGNGDTFMVHHPKYVFDLDILPIGASLWVALAQEYLK"
     gene            complement(1424..2176)
                     /locus_tag="PLI69_05560"
                     /pseudo
     CDS             complement(1424..2176)
                     /locus_tag="PLI69_05560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2216..2893)
                     /locus_tag="PLI69_05565"
     CDS             complement(2216..2893)
                     /locus_tag="PLI69_05565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG41987.1"
                     /translation="MATLSNTANATAYKGNDRVLFGFILGLLSFWLFGMTMLNINVVM
                     NQDLGLPTSSLSLAVSITALFSGMTVVVFGGLADKIGRVKITRLGFIFAIVGAALIAI
                     VPGKSGLTLPVLLTGRILQGLSAACIMPATLALLREYWDEKGRQRAVSLWSMGTWGGT
                     SFAALVGGAMADNLGWRWIFVLSTVLSVLGLFLIRDLPESKAEQKGKTQLDYLGMLLF
                     MFFIVTL"
BASE COUNT          858 a          742 c          680 g          760 t
ORIGIN      
        1 cgctacaaaa gcggcatctt ctaccatccg ggctacactt cagatgctga tattgctcaa
       61 aaggtctacg aagaggttag caaggagtat tcagttccga tatatgtcga aatcggtccc
      121 gtcacaagtt attatcctgc tgaagagtac catcagaact atctcgacaa aaatcccggt
      181 ggctactgcc acatcaaacc tcagctctac gaattcgcaa agattgtaaa caggaaatag
      241 ttgcctaaac tacttgagat actcctgagc gagagcaacc caaagagatg cacctattgg
      301 caagatgtcc aggtcaaata catatttggg atggtgtacc atgaaggtat ccccattgcc
      361 gatcatgata tagttgccgg gcttctgttg aagcatgaat gcaaagtctt cgctgctcat
      421 caaaggcttg cagggataga cgacattctc atcaccaaaa tagtgtttag ccacatctgc
      481 ggcaaacttt gtctcctccg cgctattaac caatacagct cccggctgtc cttccctgat
      541 ctcgtaagta caaccgtaac tttctgcctg agctgccgta atcttccgga tcctgtcgag
      601 tacctttttg cgggtttctg cctgcatatt gcgcatggag agtctgagaa tggcaccatt
      661 ggcgattaca ttaccctcat tgcctgcaag gaaggccccg atagtcacca cgctattgtc
      721 ccatggagag atattgcgtg aaactatagt ctgaagagcc ataacgagcg aagctcccgc
      781 aaccacaggg tctatactca actcgggcat ggagccatgg gagcctttgc caaagagttc
      841 aatctcccag ttgtctacgg cagacattgt ctcgccatca tggaagtgca gtacaccttt
      901 tggaagaccc gggatattgt gcatcgcata gacgcggtct atattgaatt tctcgaataa
      961 tccgccccca atcatagcag gaccgccctg catattctcc tccgagggct ggaaaatgaa
     1021 atgaaccgta ccattgaacc ggcgcgtttc ggccaggtac ttagccgcac ctaatgccat
     1081 tgccgagtgt ccatcatgtc cgcagaggtg agatatccca tcatatcgac tcttgtagga
     1141 gagattatta tcttcttgaa tgcgaagcgc atccatatct gcacggaagc cgatactctt
     1201 ctctccgtca ccattcttta aacggcctac tacaccatag atgccatcct tacctatctt
     1261 ttcgtgtacc tcgtagccca tttgagccag ctctttggct atatatcgtg cagtttcatg
     1321 ctctttagtg gaaacttccg ggtgggaatg gatatattgc atccaacgtt tcatctcgga
     1381 gtgcaatgcc agtactcctt caattggttt tgcgcccata tatctatgcc tcctttgttt
     1441 tacccgaaac tgttgccctg attacaagta tggcgacaac aatcatcacg aagttgaaga
     1501 gcagtgctat catggcaccg tgtctgaagt caagattgtc ctgacgtccc atgaatatcg
     1561 tgtccaaagc ggtgaatgct ctgccgttga tgacgatacc ggagtaaatg gcggtggaga
     1621 tggcgatgcc aaacccattg ccgagggaag aagccatctt atatatacct gaacctgctg
     1681 ccgtctgatc aagtggcaga ttggaaagcg ctgcatctgt cgagggagtt gcgtaaaatg
     1741 caagcccaag gccgaagaaa atataaccta tcgccatcaa taccatatag gtagaggtca
     1801 tgatattggt cggcagcata catgctatcg agagaagcag gatgagacag ccccagatca
     1861 tcggtttgcg cggaccgtac ttacgtagca accgctcacc caccctgatg aagagcagga
     1921 tcacaactcc gtatcctatc gtaaggaggc cggtagtatc cgcactgata cccccacctt
     1981 gctgcatcag cataagggta acaatgagta cgcctgaggt actattgagt atgagattgg
     2041 aaattgtagc gcctgtaaaa gtcttattct tgaagagacg aaagtcgata aagggaccct
     2101 cttttttctt tagctcaaaa ataatgaaca gtatcaaaaa taggatggag agacctgcca
     2161 aaacgagtga cttcgtgctt ccccagcccc attccgaacc cttactgaca aaaagctaga
     2221 gcgtaactat aaagaacatg aaaagcagca tccctagata gtcaagctgg gttttgcctt
     2281 tttgttccgc tttactttcg ggaagatcac ggataagaaa gagaccgagt acggaaagta
     2341 ccgtagacaa tacaaatatc catctccatc cgagattgtc cgccattgct ccgcctacga
     2401 gggctgcgaa gctggtacct ccccaagttc ccatactcca caagctgact gctctctgcc
     2461 ttcctttctc atcccaatac tctctgagta gtgcaagtgt agcaggcatg atacatgccg
     2521 cagagagtcc ttgaagaata cgaccggtaa gcagaaccgg aagcgtaagg ccactcttgc
     2581 cgggcacaat ggctatcaat gccgctccga caatggcaaa tataaaaccc aagcgtgtga
     2641 tctttaccct gcctattttg tcagccaaac ctccgaaaac cactaccgtc atgcctgaga
     2701 aaagcgcagt aatggagacg gcaaggctaa gagaagaagt tggtaaaccg aggtcctgat
     2761 tcatgaccac attgatgttg agcatggtca tgccgaacag ccagaaagag agcagaccga
     2821 ggataaatcc gaaaaggact ctgtcattcc ctttgtaggc agttgcgtta gcagtattag
     2881 atagagttgc cataatttta aaaattggtt attaggttta gattagtgaa taaattattg
     2941 tacaaatcat agaagattac caatgtgatt gattagaata gttataatgt attcagatac
     3001 aaaggtaaac ttttaaggga tttagcaaca aaaaaagcga
//
LOCUS       DAOSIE010000180         3038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_28122_length_3038_cov_4.179014, whole genome shotgun sequence.
ACCESSION   DAOSIE010000180 DAOSIE010000000
VERSION     DAOSIE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28122_length_3038_cov_4.179014"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1624)
                     /locus_tag="PLI69_05570"
     CDS             complement(<1..1624)
                     /locus_tag="PLI69_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOG41988.1"
                     /translation="MMIPTTKKEIEALGWDAPDIILFSGDAFIDHPAFGTAVIARVLE
                     DAGYRVAVVPQPNWRDDLRHFRKLGEPKLFFGVTSGAMDSMINHYTAAKRLRSNDAYT
                     PGGKASFRPDYAVTVYTKILKELFPQVPVVIGGIEASLRRLTHYDYWQDALKPSILVE
                     SGADLLVYGMGERPIRAIADALAGGVPLKALQSIPQVGYLCDSEPSVEHITLASFEQC
                     KADKREFAANFNIIEDQANLMEAEMMVERCGERYVCINSPYPPATEQEMDSIYALPFT
                     RKPHPRYRDKHIPAYEMIKHSVTTHRGCFGGCNFCTLAAHQGKFIQSRTPESILAEVE
                     ELATMPDFKGNISDLGAPTANMYRMKGRDRSLCAVCRRHSCLFPSRCENMDTSHAELL
                     SLYNKVLKIPGIKNTYIGSGIRYDLFLDKDGYLDSTSKPYLEQLIAKHTSGRLKVAPE
                     HTCDHVLKVMGKPSFKLFERLRAEFEEICHRKGLRYQLVPYFISGHPGCRMEDMQTLS
                     RNPHLKNLFMEQVQDFTPTPMTRSSVMFYTGID"
     gene            complement(1621..>3038)
                     /gene="argS"
                     /locus_tag="PLI69_05575"
     CDS             complement(1621..>3038)
                     /gene="argS"
                     /locus_tag="PLI69_05575"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG41989.1"
                     /translation="PNTNKPLHLGHIRNNLLGWSVSQLLAANGHDVVKVNLVNDRGIH
                     ICKSMLAWLKEGNGATPASTGLKGDHLVGDYYVKFNEMLTAEVKEIMEREGMEKEEAE
                     KKATLLKEAQDMLLKWESGEKEVVELWERMNGWVYDGFDVTYRNLGIEFNKTYYESQT
                     YLLGKSLVLEGVERGIFERDADGSVWCDLTEDGLDRKLLLRSDGTSVYMTQDLGTAHQ
                     RYDEYGFDEHIYVVGNEQNYHFQVLKLILKKLGFDWADSIYHLSYGMVELPEGKMKSR
                     EGTVVDADDLLENMYNTAKETSLELGKIDNMSDEEQDALFKMISLGALKYFIIKVDPK
                     KTMLFDPKESIDFNGNTGPFIQYTHARIKSILRKASEQDITPSMTGALLLPKEEAIIQ
                     LLSTYPDKIREGGDAHSPAIIANYAYDLAKEFNQYYHDTSILREEDKEMRAQRLYLIS
                     IIAQVLVKSMAILGISLPERM"
BASE COUNT          764 a          744 c          684 g          846 t
ORIGIN      
        1 ggtcgattcc ggtataaaac attaccgaag aacgggtcat cggagttggt gtaaaatcct
       61 gaacctgctc cataaaaaga ttcttcaggt gtggatttcg cgaaagagtc tgcatgtctt
      121 ccattctaca cccgggatga ccggatatga aatatgggac taactgatat ctgagtcctt
      181 tgcggtggca gatctcttca aactccgctc taagtctttc gaaaagtttg aaagagggct
      241 tacccattac ctttagcaca tggtcacaag tgtgctccgg ggctaccttt aagcgacctg
      301 aggtatgttt tgcaatcaat tgctccagat agggttttga ggtggagtcc agatagccat
      361 ctttatcaag gaatagatca taccggatac cacttccgat ataggtgttt ttgatgccgg
      421 gtatttttag aaccttgttg taaagcgata acaactctgc gtgcgaagta tccatatttt
      481 cgcaacgcga aggaaagaga caggagtgcc ttcgacacac ggcacatagt gaacgatctc
      541 tacctttcat tctatacata ttggctgtag gagcacccag gtcactgata ttgcccttga
      601 aatcgggcat tgtggcgagc tcctctacct cggcaagtat ggattccgga gttctggact
      661 gtatgaattt cccttggtga gcagctaaag tacagaaatt gcatcctcca aaacagccgc
      721 ggtgagtggt caccgaatgt ttgatcatct cataggcagg aatatgctta tcgcggtacc
      781 tcggatgtgg ctttcttgta aaggggagtg catagattga gtccatttcc tgctccgtag
      841 caggcggata aggagaattt atgcatacat aacgctctcc gcacctctcc accatcatct
      901 cagcttccat aaggttggcc tgatcctcaa taatattgaa gttggcagcg aactctcgtt
      961 tgtctgcctt gcattgctcg aatgaagcga gagtgatatg ctccacagag ggctcactgt
     1021 cacacagata tcccacctgc ggaatggatt gcagtgcttt gagaggtacg ccgccggcaa
     1081 gagcgtcagc tatggcacga atgggcctct cccccattcc ataaaccagc aggtctgctc
     1141 cactttcaac cagaattgag ggtttgaggg catcctgcca ataatcatag tgtgttaagc
     1201 ggcgcagaga agcctcaatg ccaccaatca caaccggtac ctgcggaaag agttccttga
     1261 ggatttttgt atatactgtt acggcataat cgggcctgaa agaggctttt cctccgggag
     1321 tataggcgtc attgctgcgc aggcgctttg cagccgtata gtgattgatc atagaatcca
     1381 ttgctccgga ggtgactccg aaaaagagtt tcggctcccc cagctttcta aaatgcctaa
     1441 gatcatccct ccaatttggc tgtggcacca ccgccacacg gtagcccgca tcctccagca
     1501 ctctagcaat gacggcagta ccgaaggccg ggtgatctat aaatgcatct ccggaaaaaa
     1561 gaatgatatc tggagcatcc cagccaaggg cttctatctc tttctttgta gtgggaatca
     1621 tcacatcctt tcaggcagcg agataccgag aatcgccatc gacttgacta aaacctgcgc
     1681 aattatggag atcagataga ggcgttgagc gcgcatctcc ttatcttctt ccctcaatat
     1741 cgaggtatca tgatagtact gattgaactc ctttgccaga tcataggcat agtttgctat
     1801 gattgcagga gaatgagcat ctcccccctc tctgatcttg tcgggatagg tgcttagcag
     1861 ctgaattatc gcctcttcct tgggaagcag caaggccccc gtcatggaag gagttatatc
     1921 ctgttctgaa gctttcctga gtattgactt gatacgggca tgagtatact gaataaaggg
     1981 gccggtattg ccattgaaat caatagactc tttcggatca aagagcatcg tctttttagg
     2041 atccaccttt attatgaaat atttcagcgc tccaagactg atcatcttga aaagagcatc
     2101 ctgctcttca tcactcatat tatcgatttt accgagttcg agagaggtct ctttagccgt
     2161 attgtacata ttttcgagca ggtcgtcagc atctaccacc gtgccctcac gggatttcat
     2221 tttaccttca gggagttcca ccatgccgta agagagatgg tagatggagt cagcccaatc
     2281 aaaccctaac ttttttagaa tcagtttgag tacctgaaaa tgataattct gctcgtttcc
     2341 aaccacatag atgtgttcat caaagccata ttcgtcatat ctctgatgag cggtgccaag
     2401 atcctgtgtc atgtagaccg aggtaccgtc acttctgaga agcagtttcc tgtcaagtcc
     2461 atcttcagta agatcgcacc agaccgaacc atcggcatct ctttcaaaaa tgcccctttc
     2521 tactccctcc agcacgagag atttaccgag cagataggtc tgagactcat aataggtctt
     2581 gttgaattct attccgagat tacgataggt aacatcgaag ccatcataga cccatccatt
     2641 cattctctcc caaagctcca ccacctcttt ttctccgctt tcccatttca ggagcatatc
     2701 ctgggcctct ttaagcaggg tagccttttt ttcagcctcc tctttttcca tgccctcacg
     2761 ctccattatc tccttcactt cagcggtaag catttcgttg aatttcacat aatagtcacc
     2821 cacaagatgg tcacctttaa gtcctgtaga ggcaggagtg gctccattgc cctctttaag
     2881 ccatgcgagc atcgatttgc aaatatggat tcctctgtca ttgacgagat tgacttttac
     2941 aacatcgtgt ccgtttgctg ccaggagttg tgaaacggac catccgagga gattattgcg
     3001 aatatgtccc agatggagcg gtttattggt gttgggtg
//
LOCUS       DAOSIE010000181         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_28176_length_3035_cov_3.277181, whole genome shotgun sequence.
ACCESSION   DAOSIE010000181 DAOSIE010000000
VERSION     DAOSIE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28176_length_3035_cov_3.277181"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLI69_05580"
     CDS             complement(<1..187)
                     /locus_tag="PLI69_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41990.1"
                     /translation="MITTLIVIGIIAILVIWVISVQRNLISKEELCKNSMSQIGVQMS
                     SRWDALTALADLIKSYDE"
     gene            complement(313..1539)
                     /locus_tag="PLI69_05585"
     CDS             complement(313..1539)
                     /locus_tag="PLI69_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41991.1"
                     /translation="MRGRVFLISVLAVVILTGCSTGDKRPPMETGITENPYKGYRTRA
                     DFGLNGPVKSFTETRYNDLQPFSRTWYFDPNGRVISIVEEDVHPQTRLEISIDKKGNP
                     VDTIFSFTESLKQAFDLKASTYLPDGKPLKEVYRMDGMLAEEHRFTYDKNGLCIRIDI
                     VESNGELRGSFLNTYDENGNLTNERYRSIKYAVDGNEIISEIINVYDDKGNLIESSNY
                     NLGELDVRDVKYYNEKGDLLRHYSEYKDGFATEIFYTYDKDGRSNGNSFYSLEDDRRV
                     IMARDSTFIDEAGYRHEEHHTWTGGLHTSEAIFDKKGKMVEYNYYSEFTRKSRIPENS
                     ITFHYDDEGYLTKAELKSGETIIPYSFGEKDRFGNWMRRSIPPDEVFDTFGIGIEEFS
                     HFLIHYERKYTYYEED"
     gene            complement(1550..2746)
                     /locus_tag="PLI69_05590"
     CDS             complement(1550..2746)
                     /locus_tag="PLI69_05590"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HOG41992.1"
                     /translation="MAYTTTTKQSYGNRVSKSFKGIGSGILLFIIGTVLLFWNEGRTV
                     KVKRALNQAEDVTVEMPDISRADPQFEGKMIHATGDASTAEILTDDLFGISLNAINIS
                     RNVEYYQWTEHSKEEKKEKIGGGQEITTTYTYSKEWVSSPVNSSEFADPSYQNSNFVL
                     TTIDSKQVVADVVDFGAYKLPPFLKNGIRSAEVATPQLTEEMIKEWNEVLWKRDSSAT
                     VSVSTGQAYFGRDINNPEVGDIRVTFNYTPTPQTVSILAVVVGNTFAEFVAKNGKNVS
                     RIELGAVPAANMYEAAQKENKIIAWIVRILAVLLIIGGLKGIFNFLSTIFAVVPFIRR
                     VIAAGINFVCTIIGLIWSLLIFVIAWISYRPALGITFLVLIAALIVLLVMRSRKKKSA
                     AANTTA"
     gene            complement(2817..>3035)
                     /locus_tag="PLI69_05595"
     CDS             complement(2817..>3035)
                     /locus_tag="PLI69_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG41993.1"
                     /translation="VRRQAEIAGIRKNISPHTFRHSFASHLVENGADLRVVQEMLGHE
                     SILTTEIYTHIDTKKWQQTILQFHPKRQ"
BASE COUNT          794 a          658 c          641 g          942 t
ORIGIN      
        1 gttcgtcata ggatttgatg aggtccgcca gtgccgtaag ggcatcccaa cggctagaca
       61 tttggacgcc tatctggctc attgaatttt tgcacaattc ttctttgctg ataagattcc
      121 tctgtacgga aatcacccaa attaccaaaa tggcaataat gccaattaca atcaatgttg
      181 ttatcatata atgaagttta atgtgttgtt aaatgttgta tctataatgt gctaaaggta
      241 tttgcaatca gcctgtccgg tgatcttctc atattccgga gtggagccgt cttgcattta
      301 taatttgtta tcctaatcct cctcgtaata ggtatatttt ctttcatagt gtattaggaa
      361 atggctaaac tcttctatgc cgataccgaa ggtgtcaaat acctcatccg gaggaatgga
      421 acgtctcatc cagttgccga aacgatcttt ttctccgaaa gagtagggaa taatagtctc
      481 tccgctctta agctcggctt ttgtgaggta gccctcgtcg tcataatgga aagtaatgct
      541 attctcagga atgcgggatt tccgagtaaa ctcgctatag tagttgtatt ccaccatttt
      601 cccctttttg tcgaagattg cctcactggt atgtagacca cccgtccagg tgtggtgttc
      661 ttcgtgtctg tatccggcct cgtcaatgaa ggtgctatcc ctagccatta tcacacgtcg
      721 gtcatcttct agggagtaga aactattgcc gttacttctg ccatctttgt cgtaagtgta
      781 gaagatttcg gtagcaaagc catccttata ctctgaatag tggcgtaaaa gatctccttt
      841 ctcattataa tacttgacat cccttacatc aagttcaccg aggttgtaat tagatgattc
      901 aattagattg cctttatcgt cgtaaacatt tataatttct gaaatgattt cgttgccatc
      961 gacagcgtac ttaatgcttc tataccgttc gtttgtcagg ttgccatttt catcataggt
     1021 attgaggaat gagcctctaa gctcaccgtt actctcaaca atgtcgattc ttatgcagag
     1081 tccatttttg tcataagtga atctatgttc ttcagccagc attccatcca tcctatatac
     1141 ctccttgaga ggcttaccgt caggaagata agtgctggcc ttcaaatcaa atgcctgctt
     1201 aagagactct gtaaaggaga atatcgtatc gacagggttc ccttttttat ctatacttat
     1261 ttccagacgt gtctgggggt ggacatcttc ctccacaata gaaattactc tgccgttagg
     1321 atcaaaatac catgtgcgtg agaaaggttg taaatcattg tatctggtct cggtaaatga
     1381 tttaacggga ccattgagtc caaaatcagc acgcgtccta tagcctttat agggattttc
     1441 ggttatgccg gtctccatcg gaggtcgctt atctcctgtt gagcatcccg tcaaaataac
     1501 taccgccagg acagatatca aaaatactct tcccctcatc tgaaaaatat taggcggtag
     1561 tattggcagc tgcacttttc tttttcctgg aacgcattac gagcagcact atgagtgcag
     1621 ctatcaaaac aaggaaggta attccgagtg ccggtcgata ggatatccag gctatgacga
     1681 atatcagcaa actccagatt aacccgatta tcgtgcagac aaaattgatg cctgcagcga
     1741 taaccctgcg aataaaggga acaactgcga atatggtgct caggaagttg aagataccct
     1801 tgagaccgcc tataatgagt agaactgcaa ggattctaac gatccaggct atgattttgt
     1861 tctctttctg tgctgcttca tacatattcg cagcaggaac cgctccaagt tcaatacggg
     1921 agacattttt gccgttttta gctacaaact cagcgaatgt gttgccaacg accactgcaa
     1981 gtatcgacac cgtctgcgga gtgggagtat aattgaatgt caccctgatg tcacccactt
     2041 ctgggttgtt gatatcgcgt ccgaaataag cctgtcctgt gctaactgaa acggtagcag
     2101 aactgtctct cttccagagt acctcattcc actcctttat catctcttcg gtaagttgag
     2161 gtgtagctac ctccgcactc ctgattccgt tcttaagaaa gggagggagt ttgtatgccc
     2221 cgaaatctac tacgtcggca acaacttgct tcgaatcaat ggttgttagt acaaagttgg
     2281 agttttggta agaaggatcg gcaaactcgg aggaatttac aggggaagag acccattctt
     2341 tgctatatgt gtaagtggtg gtaatctcct gaccgccccc tattttctct tttttctcct
     2401 ctttgctatg ctcggtccac tggtagtact ccacgttacg ggagatattt atcgcattta
     2461 atgagatgcc gaagaggtca tcggtaagga tttcagcagt agatgcatct cccgtagcat
     2521 gtatcatttt accttcaaac tgggggtctg cgcgggagat atcgggcatc tctacagtta
     2581 catcttctgc ctggtttaaa gcacgtttaa ctttgacagt tctgccctca ttccaaaaca
     2641 acagtaccgt accgatgata aaaagcagga taccgctgcc gataccctta aaagacttgg
     2701 aaactctgtt tccatagctc tgtttggttg tagttgtgta agccatacaa ttattaagtt
     2761 aagttttata ttattgatag ttaattttaa aatccaattt gtaaagttag gaaaaattat
     2821 tgtcttttag ggtgaaattg gagaatagtt tgctgccatt tctttgtatc gatatgtgtg
     2881 taaatctcag tagtcagtat gctttcgtgg ccgagcattt cttgaacaac acgcaaatca
     2941 gctccatttt ccaccagatg agaagcaaaa gagtgtctga aagtgtgcgg ggagatattt
     3001 ttgcggattc cggcgatttc agcctgccgg cgaac
//
LOCUS       DAOSIE010000182         3030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_28227_length_3030_cov_3.725042, whole genome shotgun sequence.
ACCESSION   DAOSIE010000182 DAOSIE010000000
VERSION     DAOSIE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28227_length_3030_cov_3.725042"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PLI69_05600"
     CDS             complement(<1..1040)
                     /locus_tag="PLI69_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41994.1"
                     /translation="MKKVLIITLALSATFLSCKKNVPQAIINLPIIDRALHDSTSVFY
                     GDFEEYPEDLKMLPIGVFDSGTGGLTVLEKIVSLDVFDNITGKMGDDDILDFAGEHFI
                     YLADQANMPYGNYDAEGKSDYLRELVVKDALFLLDDNYYETQYDREANGVKPRVKIIV
                     IACNTATAYGLKDIASLLEQSKTGVKVIGVINAGVKATLDELNVSEGDQPFAIGVLAT
                     PGTIASGAYQRTIEEMVAERGIKTNVTVVNQKGYGFAEAVDSEPDFVNPALIFPRDSY
                     RGPRIGQGDESIDINLMRIYNFDFAGYRMLYKRDAQGSYKEIQLNDAVNYARFNLVSL
                     VEKHRLSGSIAPL"
     gene            complement(1043..2224)
                     /locus_tag="PLI69_05605"
     CDS             complement(1043..2224)
                     /locus_tag="PLI69_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOG41995.1"
                     /translation="MNNTEANRYAARGVSSSKEDVHKAIADMDKGLFPKAFCKIVPDF
                     LSNDPEYCVVMHADGAGTKSSLAYIYWKETGDMSVWKGIAQDAIVMNTDDLLCVGVTD
                     NILISSTIGRNKQLIPGEVISALIGGSQEFVRKMADLGIGLLLTGGETADVGDLVRTV
                     IVDSTVCALMRRSDVIDNGRICAGDVVVGLASFGQATYEEEYNGGMGSNGLTSARHDM
                     FEHSLAAKYPESFDNSVDEQYVYCGSKSLTDLEPESGLTYGKLVLSPTRTYAPVIKEV
                     LKNYRPQVHGMVHCSGGAQTKVLHFIEGLKVIKDNLFPTPVLFRTIQQESGTPWSEMY
                     KVFNMGHRMELYVPENIARDIISISEGFGIEARIVGKVEKSHQAEVEIISEQGKFNYR
                     R"
     gene            2346..>3030
                     /locus_tag="PLI69_05610"
     CDS             2346..>3030
                     /locus_tag="PLI69_05610"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOG41996.1"
                     /translation="MRTKLIAALLLLPGLLFAQNRVQNYINRQLKTDSLFINAAVGIL
                     AVDENGKDIASWNGEMPLLTASTMKTITTGVAIEVLGPDFKFSTKVAYDGYIMDDGTL
                     NGDLYIVGGGDPTLGSHDKIAFPIDSIFAIWADAIAEAGIKRINGSIIGDDRFFIDEN
                     IPDSWAYGNLGYYYGSGPSGLSYAENLSRFEIEPGATVGDPVKITPLPPYSPKMTYIN
                     EVITGPEKSR"
BASE COUNT          772 a          771 c          635 g          852 t
ORIGIN      
        1 agaggtgcta tagaaccact aagacgatgt ttctctacca gtgaaacgag gttgaagcgg
       61 gcgtagttga cagcatcatt gagctggatc tctttgtaac taccttgagc atctcgctta
      121 tagagcattc tgtagccggc aaaatcgaaa ttataaatcc tcattaaatt aatgtctatg
      181 gactcatctc cctgccctat acggggaccc ctgtatgaat cgcgcggaaa tataagggca
      241 gggttgacaa aatcaggctc gctgtccaca gcctcggcaa atccgtaccc tttctggttg
      301 acaactgtca catttgtctt aatgccacgc tctgctacca tctcttcaat tgtgcgctga
      361 tatgcaccgg atgcaattgt accgggagtt gcgagtactc caatggcaaa cggctgatca
      421 ccttcactca cattcaactc gtctagagtt gcctttactc cggcattgat tactccaatc
      481 acttttacac cggtcttgct ctgctccagc agcgaagcta tatccttcaa cccatacgca
      541 gtagcggtat tgcaggctat tacgatgatt tttacgcggg gtttaactcc gttggcctct
      601 ctatcgtatt gtgtttcgta gtaattgtca tcgagcagga aaagcgcatc tttgactacc
      661 agttctctaa gataatcgct cttgccttct gcatcataat tgccgtaggg catattggcc
      721 tgatctgcga gatatatgaa atgttctcct gcaaagtcca ggatatcatc atcgcccatt
      781 ttgccggtta tattgtcaaa aacatccaga gatacgattt tttcgagaac cgtcaaacct
      841 cctgtgccgg aatcgaagac tccgatgggc agcatcttga gatcttccgg gtactcttca
      901 aaatctccat aaaatactga ggtgctgtca tggagagctc tgtcgatgat gggcagattg
      961 atgatcgcct gtggaacatt tttcttgcag gacaaaaaag tcgcggataa tgcaagtgtg
     1021 atgatcagta cttttttcat aattatcttc tatagttgaa tttcccctgt tctgaaatta
     1081 tttctacctc agcttgatga cttttttcca cttttcctac tatacgcgcc tctatcccga
     1141 atccttcgga aatggagata atatccctcg ctatattttc aggaacatat agttccatcc
     1201 tatgtcccat attgaacacc ttatacatct cactccatgg agtacctgat tcctgttgga
     1261 tagtcctaaa gagcaccggt gtagggaaga ggttgtcctt gatcaccttc aggccctcaa
     1321 tgaaatgaag caccttggtc tgggcaccac ccgagcagtg taccataccg tggacctgag
     1381 ggcgataatt cttcagtacc tctttaatta ccggtgcata ggtgcgtgtg ggagagagta
     1441 ccagtttacc gtaggtcagt ccgctctcag gctctaaatc ggtaagggac ttgctcccgc
     1501 aataaacata ttgttcatca accgagttgt caaaactttc aggatatttt gcagccaggg
     1561 agtgctcaaa catatcgtgg cgtgctgaag taagcccatt gctacccatc ccgccgttgt
     1621 actcctcttc gtatgtggct tgtccgaagg atgccagtcc taccaccaca tcaccggcac
     1681 aaatacgtcc attgtctatc acatcgctac gacgcataag ggcacacacg gtactgtcaa
     1741 ctatcacggt ccgtaccaaa tcccctacat ccgcagtctc gccgcctgta agcagcagcc
     1801 cgatgcctaa atcagccatc tttctgacaa attcctgcga tccgccaatg agtgcgctga
     1861 ttacctctcc cgggatgagt tgtttgtttc gtccgatagt ggaggagatt aagatattgt
     1921 ctgtgacccc aacacagagc aggtcgtcag tattcatcac aatcgcatcc tgagctatac
     1981 ctttccagac actcatgtcg cctgtctctt tccagtaaat ataagcgaga gaacttttgg
     2041 tacctgcacc atcagcgtgc atcaccacac agtattcagg atcattactc aggaaatcgg
     2101 gtacgatctt gcagaacgct tttgggaaaa gccccttatc catatcggct atagccttat
     2161 gcacatcttc tttggaagag gaaacccccc ttgcagcata tctgttggct tcggtattgt
     2221 tcatatctcc tctaaaactg cccattgcta tctctttttt gaataatatt ccacaaaatt
     2281 acttgtttta ttgaaacaat ccctcttttt tgctattttt gtccacctca ataaagcaca
     2341 acactatgcg tactaaacta attgccgccc ttttactttt gccggggctt ctttttgccc
     2401 agaaccgggt acagaattac atcaaccgac aactcaagac cgactcactc ttcatcaatg
     2461 cagcagtcgg catccttgcc gtggatgaaa acggaaaaga tatagcttcc tggaatggag
     2521 agatgccact gctgacggct tctacgatga agaccataac cacgggagtg gccattgagg
     2581 tactcggtcc tgacttcaag ttctctacaa aggtggctta tgacggctat atcatggatg
     2641 acggtaccct taatggcgat ctctacatag tcgggggagg tgatcctacc ttagggtcac
     2701 atgataagat agcatttccc atagattcta ttttcgcaat atgggcagat gcaatagccg
     2761 aagccggcat taaacgcata aatggcagca ttatcggtga tgaccgcttc ttcatagatg
     2821 agaatatccc tgatagctgg gcttacggca atctgggcta ctactatggc agcggtcctt
     2881 ccggcctttc atacgccgaa aacctcagcc gttttgagat tgagccggga gcaacagtgg
     2941 gagatccggt taaaatcaca ccgcttcctc catactcacc taaaatgacc tatatcaacg
     3001 aggttattac cggccctgaa aagagtcgtg
//
LOCUS       DAOSIE010000183         3015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_28408_length_3015_cov_4.177027, whole genome shotgun sequence.
ACCESSION   DAOSIE010000183 DAOSIE010000000
VERSION     DAOSIE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28408_length_3015_cov_4.177027"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLI69_05615"
     CDS             <1..462
                     /locus_tag="PLI69_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG41997.1"
                     /translation="STAPLRAVILGCTHYPFLIETLWQVIDELKNVKVEGQYLYKHLI
                     ADDFLFIDPAVYTAIECYMTLREEGNLALRIGEQRVNGYISVPDGFLDGKYLTSDEKL
                     TYEFKYGRDVDSQIIATKQVPFSKSNIDSNNLLRIKLLLPYSYELIYPTLY"
     gene            462..1070
                     /locus_tag="PLI69_05620"
     CDS             462..1070
                     /locus_tag="PLI69_05620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG41998.1"
                     /translation="MLNEHITPEEIECLEIISFPGEINVISTTGKKFSEAIKHLREQI
                     FIGFDTETKPNFNANTPRHKTALLQLASETKAFLFRVNKIGLPQELADLLSNPEITKV
                     GAATTDDIRGLRCYREFEPRRFIDLQDFVEQYGILEKSVRKLAAIILGKRISKAQQLS
                     NWEAAKLTPAQQLYAATDAWICVKMYKKLLASPKVPLQENEV"
     gene            1067..2248
                     /locus_tag="PLI69_05625"
     CDS             1067..2248
                     /locus_tag="PLI69_05625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOG41999.1"
                     /translation="MKRVHLKKGREESLLRRHPWLFSGAISRMEGEPNEGDIVSVISQ
                     NGELLGYGHFQIGSIAVRILSFDDAALSPYFWKERISAAYRHRLSISLKNTDCYRLVH
                     GEGDGLPGLIVDWYNGVAVVQAHSVGMFLSLEKISNALKEVYGEQLMAIYDKSSVTAP
                     FNAGLDLSDGYLYCNDAFESAKEGVVVENGHKFIVSWEEGQKTGFFLDQRENRALVER
                     YASGRKVLNLFSYTGGFSVYALAGGAASVDSVDSSSKAVAMMERNVALNALSNHRSFC
                     EDAIDFVRNSPEGKYDMIIVDPPAFAKHRGALRNALKGYQRLNSAAISKVAPGGIIFT
                     FSCSQVVDSYSFALAIFSAAAEAGRSVKILDRLNQPADHPVNIFHPEGEYLKGLVLYV
                     E"
     gene            complement(2281..>3015)
                     /locus_tag="PLI69_05630"
     CDS             complement(2281..>3015)
                     /locus_tag="PLI69_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOG42000.1"
                     /translation="EVMVSFDEFDGALYSADAFGKFGALSKFGFYSSEDSDWSEEARR
                     YYINIVGKYGGPVQTLLKKASTLPIKSIRPLHGPIINEDLEKYIKLYDTWSRCIPESK
                     GIMIAVASIYGGTMEAAEKLKELLESKGAENVFIRDLCRTDVSRNVSDAFRYEKLVLA
                     ASSYDNGLFTPMHSFIHILQMKCFMGRTIGLIENGSWAPSAARVMNEMLCQMTNITIV
                     EPTVTIRSRMKSTDYPALERLADALL"
BASE COUNT          845 a          697 c          694 g          779 t
ORIGIN      
        1 tctacagcac ctcttagagc ggtaattttg ggatgcaccc actacccttt cctgatagag
       61 acactttggc aagttataga cgaactcaaa aacgttaaag ttgaagggca atatctctac
      121 aaacatctga ttgcagatga tttcttattt atagaccccg cagtctatac tgcaattgag
      181 tgctacatga ccctgcgcga agagggaaac cttgctctgc gcatcggaga gcaaagagtc
      241 aatggatata tctccgtccc tgatggattt cttgacggca aatacctcac ttcggacgaa
      301 aaactcacct acgaattcaa atatggaagg gatgtggatt cacaaatcat agcaaccaaa
      361 caggtaccct tttcaaagag caatattgac agtaacaacc tcctgcgtat caagttactg
      421 ttgccctatt cttacgaact catctatccc acactctact gatgttaaac gagcatatca
      481 cacctgaaga gatagagtgc ctggagatca tttcatttcc cggagaaatc aatgtaattt
      541 caactaccgg aaagaaattt tcagaggcca taaagcatct gagggaacaa atatttatcg
      601 gttttgatac cgagacaaaa cctaacttca atgccaatac cccaaggcac aaaacagcat
      661 tgcttcaact ggccagtgag acaaaagcat ttctgtttag agtaaataag atcgggctac
      721 cgcaggagct ggctgaccta ttgtctaacc cggagataac caaagtgggg gcagccacaa
      781 cggatgatat caggggacta cggtgttaca gagagtttga accgcgaaga tttatagatc
      841 ttcaggactt cgtcgaacag tacggcatac tcgagaagag tgtacgcaag ttggcagcca
      901 tcatcctcgg caaaaggatc tccaaggcgc aacagctctc caactgggag gcggctaaac
      961 tgacccccgc tcagcaatta tacgcagcca ctgatgcctg gatttgcgtt aaaatgtaca
     1021 aaaagctcct cgcctctcct aaagttccac tacaagagaa cgaagtatga aacgcgtcca
     1081 tctcaagaaa ggcagggaag agtccctgct gcgtcgtcac ccatggctct tctccggtgc
     1141 aatatctcgt atggaaggtg aaccgaatga aggagatata gtatctgtaa tcagccaaaa
     1201 cggtgaactt ttaggctacg gacattttca gataggttcg atcgcagtac gaattctcag
     1261 ttttgatgat gctgctctct ccccttattt ctggaaggag aggatctcag ctgcatatag
     1321 acatcgactc tctatctctc tgaagaatac cgactgctat cgtctggtgc atggagaggg
     1381 agatggtcta cccggcctaa tagtggattg gtacaacgga gtggcggtgg tgcaggcaca
     1441 ttcggttggt atgtttctct ctttggaaaa gatttccaat gccctaaaag aggtttatgg
     1501 agagcagctc atggcaattt acgataagag ttcggtgact gctccattca atgccggatt
     1561 agaccttagc gatggttatc tctattgcaa cgacgctttt gaaagcgcta aagagggtgt
     1621 ggttgtggag aatggacata aattcatagt aagctgggag gagggacaaa agaccggctt
     1681 ctttctggat cagagagaaa acagggcact tgtcgagaga tatgcttccg ggcgcaaagt
     1741 actcaatctt ttctcttaca ccggtggatt ttccgtatat gcgctcgccg gcggagctgc
     1801 aagtgtggat tccgtagata gttcctcaaa agcggttgcc atgatggaga ggaatgtagc
     1861 cctcaacgct ctttccaacc atcgttcctt ctgtgaggat gctattgact tcgtaagaaa
     1921 ttctcctgag ggcaaatacg atatgattat agttgaccct cccgccttcg cgaaacaccg
     1981 gggtgcactc agaaatgcac tcaaaggcta tcaaagacta aatagtgcgg caatatccaa
     2041 agtagctccg ggaggtatca tttttacttt cagctgctcg caagtagtgg atagctactc
     2101 tttcgccctc gccatttttt ctgcggcggc agaagcggga aggagcgtaa aaatactcga
     2161 ccggctcaat cagccggccg atcatcccgt aaatattttc catccggaag gagagtatct
     2221 caaaggtctg gtactctacg tcgaataaaa acctcttctg ccggaggcag aatcttgttt
     2281 ttataaaaga gcatcggcga gcctctcaag tgcaggatag tctgtactct tcatacggct
     2341 tcttatggta acggtaggct caactatggt gatattggtc atctggcaga gcatttcatt
     2401 cataactctc gctgctgagg gggcccacga gccgttttct ataagcccta ttgtgcgtcc
     2461 cataaaacat ttcatctgga ggatatgaat gaaactatgc atcggggtaa agaggccatt
     2521 gtcataagag gaggctgcaa gtaccagttt ctcgtaacgg aatgcatctg aaacattgcg
     2581 agaaacgtca gtgcgacata agtctctgat gaatacattt tccgctccct tagactcaag
     2641 cagctctttc agtttttccg ctgcctccat agttccaccg taaatggagg ccactgctat
     2701 cataatgccc tttgactccg gaatacacct gctccatgta tcgtagagtt tgatatattt
     2761 ttcaagatcc tcatttataa tgggaccatg aagcggacga atggatttga tggggagggt
     2821 tgaggctttt ttcagaagag tctggacagg gccaccatac tttcctacaa tattgatata
     2881 atagcgtcgc gcctcttccg accagtcact atcttcgctg ctatagaatc cgaattttga
     2941 aagtgcgcca aatttgccga aagcatctgc actgtaaagt gcaccgtcaa actcatcgaa
     3001 cgataccatc acttc
//
LOCUS       DAOSIE010000184         2995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_28667_length_2995_cov_4.025850, whole genome shotgun sequence.
ACCESSION   DAOSIE010000184 DAOSIE010000000
VERSION     DAOSIE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28667_length_2995_cov_4.025850"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLI69_05635"
     CDS             <1..454
                     /locus_tag="PLI69_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOG42001.1"
                     /translation="REDAICEEKKAVFIIGIGGELSDGKPHDSRASDYDDWSTPNSDG
                     YNGLNGDLLLWNPVLGSAFEISSMGIRVDEEALRYQLALRGEEWKSELYFHRKLLAGE
                     LPLSIGGGIGQSRLCMFLLRKVHIGEIQSSIWPEEMRIECHKNGIDLA"
     gene            complement(494..2170)
                     /gene="ettA"
                     /locus_tag="PLI69_05640"
     CDS             complement(494..2170)
                     /gene="ettA"
                     /locus_tag="PLI69_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOG42002.1"
                     /translation="MADEKIIFSMNGVSKILPHNNKVILKDIYLSFFYGAKIGIIGLN
                     GSGKSTVLKIIAGVEIPTQGEVVFAPGYSVGYLEQEPQLDDSKTVIEVVREGVQEVMD
                     LLSEYEEVNLKFMEPMDDDEMNALIERQGELTDRIEQVSGWDIDSRLERAMDALQCPE
                     PDASISTLSGGERRRVALCRLLLKEPDVLLLDEPTNHLDTESIQWLEAHLKQYKGTVI
                     AVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKSIRLAMEEKQESKRRKTLER
                     ELEWVRMAPKARHAKSKARLGAYEKLLSEDGRQKDERLEIFIPNGPRLGDKVIDCQGV
                     SKAYGDKILFEDLTFKLPPAGIVGVIGPNGSGKTTLFRLIMGYDKPDSGTFEIGESVK
                     IAYVDQQHKQIDPDLTVYETIAQNSEYVKLGNREVNARAYVSRFNFGGADQEKLCGVL
                     SGGERNRLHLALTLKENANVLLLDEPTNDVDVNTIRALEEGLENFAGCAVVISHDRWF
                     LDRIATHILAFEGDSKVYFFEGTYSEYEENKKRRLGNVEPKRFKYKKLMG"
     gene            complement(2177..2803)
                     /locus_tag="PLI69_05645"
     CDS             complement(2177..2803)
                     /locus_tag="PLI69_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOG42003.1"
                     /translation="MNNFVITIGRQIGSGGLATARMLSEQLGVKMYDKNLLLEVSKQS
                     GLACECFEKRDEKRAKRPLVRFFGLRSTMQGNDAFSSFNAISDEELFKIQSEVMRCIA
                     ERESCIIVGRCADYVLRDHPRRLSVFISASKEDRVARLVSDQGISEAEACRLIERGDR
                     KRSDYYNYYTFRQWGDCSSYHLSLNSTKLGSIENVVKVIRNYMEIINF"
BASE COUNT          747 a          767 c          667 g          814 t
ORIGIN      
        1 gagagaagat gcaatttgcg aggagaaaaa ggcagtcttc ataatcggca tcggcggaga
       61 actctctgat ggaaaacctc acgactcgag ggcatccgac tatgatgact ggagtactcc
      121 gaattcggat gggtataatg ggctgaacgg agacctgctg ctatggaatc ctgtactggg
      181 gagtgccttt gagatttcct ccatgggtat aagggtagat gaggaagctc tgcgttacca
      241 acttgctctc agaggtgaag agtggaagtc tgaactctac ttccaccgaa agctgcttgc
      301 cggagaattg cctctttcca taggcggtgg cataggacaa tcacgcctct gcatgttcct
      361 gctaagaaaa gttcacatag gcgaaattca gagcagtatc tggcctgaag agatgcgcat
      421 tgagtgccat aaaaacggca tagacctggc ctgagataag catagagttc gcctaaattg
      481 gcttaaaagc cacctatccc ataagtttct tgtatttgaa gcgtttaggt tctacattac
      541 ccagacgacg cttcttattc tcctcatatt ccgagtaggt cccttcaaag aaatagacct
      601 ttgagtcacc ctcaaatgca aggatatggg ttgcaatacg gtcgagaaac catctgtcgt
      661 gagagatgac tacggcacaa cctgcaaaat tttcgagacc ctcctcgagg gctcggatag
      721 tgttgacatc cacatcgttc gtaggctcgt caagcaacag cacgtttgca ttctccttta
      781 gggtcaatgc aaggtggaga cgattcctct ctcctcccga gagaaccccg caaagttttt
      841 cctgatctgc ccctccaaaa ttgaatctcg aaacgtatgc acgggcatta acctcacgat
      901 tgccaagctt tacatattcg gaattctgcg caatggtctc gtaaactgta agatcgggat
      961 caatctgctt gtgctgctga tccacatagg ctattttgac actctctcct atctcaaagg
     1021 ttccgctatc aggcttatca tatcccataa tcagcctgaa aagtgtggtt ttgccggatc
     1081 cgtttggacc aattacacct acaattcctg ccggaggcag tttaaaggtc aaatcctcaa
     1141 ataatatctt gtcaccataa gccttgctca ctccctggca gtcgatcacc ttgtcaccaa
     1201 gacggggacc atttgggata aagatttcaa gcctctcatc tttctggcgt ccatcttcgc
     1261 taagaagttt ttcgtaagca cccaaacggg ccttagactt ggcatgacga gcttttgggg
     1321 ccattctgac ccattcaagc tcccgctcga gggtcttacg acgcttactc tcctgtttct
     1381 cctccattgc aaggcgaatg cttttctgct caagccagga tgaataatta cccttccagg
     1441 ggattccctc tccacggtcc agttcgagta tccatccggc tacgttgtcg agaaaatatc
     1501 tgtcgtgagt cacagctatg accgtcccct tgtactgctt gagatgtgct tcgagccact
     1561 ggatactctc ggtatcgagg tggttggtgg gctcatcaag caataatacg tccggctcct
     1621 tgagtagaag gcggcaaagt gccaccctgc gcctctctcc accactgagc gtagaaattg
     1681 atgcatccgg ctccgggcac tgaagcgcat ccatcgccct ttccagcctg gagtcaatat
     1741 cccaaccgct tacctgctcg atcctatcgg taagctcacc ctgccgctca atgagagcgt
     1801 tcatctcatc atcatccatc ggctccatga atttgaggtt cacctcttcg tactcgctaa
     1861 gcaagtccat tacctcctgt actccttccc taaccacttc tatcaccgtc ttggagtcat
     1921 caagctgcgg ctcctgctcc agatatccca cggagtatcc gggagcaaac actacttctc
     1981 cctgtgttgg aatctcgaca ccggctatga tcttcaatac ggtggattta cccgatccgt
     2041 taagacctat gatgccgatt tttgctccgt aaaaaaacga gagataaata tcctttaaga
     2101 ttactttgtt gttgtgggga agaattttgc ttaccccgtt catcgaaaag ataatttttt
     2161 cgtcagccat gatatgttaa aaatttatta tttccatgta attgcgaata accttcacta
     2221 cattttctat actgcctaat tttgtggagt tgagagagag atggtaactg gaacaatctc
     2281 cccactgtct gaaggtgtag tagttgtaat aatcggaccg cttacggtct ccccgctcta
     2341 tcaggcggca ggcctcggct tcagatattc cttgatctga gacaagcctg gcaactctgt
     2401 cctctttcga ggcactaatg aaaacagaaa ggcgacgggg atgatcgcga aggacatagt
     2461 cagcacatct tcccactatg atgcaggatt ctctttctgc aatacacctc ataacttcgc
     2521 tctgtatttt aaatagttct tcgtctgaga tggcattaaa cgaagagaat gcgtcatttc
     2581 cctgcattgt cgacctaagt ccaaaaaaac gcacaagagg cctcttggct cttttttcat
     2641 cgcgtttttc aaagcattcg caagccaggc cgctctgttt ggagacttcg agcaacaggt
     2701 tcttatcata catctttacg ccgagctgct cggatagcat acgtgccgtg gcaagtcctc
     2761 cactgccaat ctggcgtcct atagttatta caaaattgtt catatcgagt tacgggttaa
     2821 ggggtgcgag ttacggggtt cgggatgggt tcttcttcaa tattctgaag ggctgtttca
     2881 gccctgaatt tacgaatcaa agaggcaagc atcagtgctg ccactatgga tgcaatggca
     2941 tcagagaccg gcatgctgat ccatactccg tcaactccga aaaagccggg cagta
//
LOCUS       DAOSIE010000185         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_28718_length_2991_cov_4.020436, whole genome shotgun sequence.
ACCESSION   DAOSIE010000185 DAOSIE010000000
VERSION     DAOSIE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28718_length_2991_cov_4.020436"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /gene="mutS"
                     /locus_tag="PLI69_05650"
     CDS             <1..1393
                     /gene="mutS"
                     /locus_tag="PLI69_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG42004.1"
                     /translation="LLDIQQREKERTGIPSLRISYNNVFGYFLEVRNTHKERVPSDWI
                     RKQTLVNAERYITPELKQYEEKILGAEEKILALESEIYGALLASIQAEVALIQRNASV
                     VSRLDVLAGFARLALDNRYCRPQVDDSLEIEIEGGRHPVIETRMAPGEEYVPNDLYLN
                     NDSQQIIILTGPNMSGKSALLRQTALIVVMAQIGSFVPANRVKMGITDKIFTRVGASD
                     NIARGESTFMVEMIETATILNNLSQRSLVLLDEIGRGTSTYDGMSIAWAIVEHLHGGT
                     QRAKTLFATHYHELNDLEALHERVFNYHISVRESEGKIIFLRKLCRGGVAHSFGIHVA
                     RLAGMPDDVVKRAEARLSELEREGGAAGGTLAEAPSDSSSIGTQEETPSDSSSIGTRS
                     SDINVGSATFSRKTKKSARSTPDTGLQLTLYQLDDPLLLDIKEELQNIDINKMSPLDA
                     FDTIRRLKKKIGL"
     gene            1419..2654
                     /locus_tag="PLI69_05655"
     CDS             1419..2654
                     /locus_tag="PLI69_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG42005.1"
                     /translation="MRELLADKFRTTLSLAGVSIGIFSIVAVFTVVDSLKESVNEGFR
                     KFGSDVVMVERVPLEPDFDQDGRQKWWQYESRPQITWQEYLFLKEKSTTAGRMLFSAS
                     FEMECAAGRESYKEGAFVAVEGDWRMMVQENILYGRGFTSGELSGGAAVTIIGSDVAQ
                     KLFPDITDPVGRMITVGGTPLRVIGLFAPSGNNLVSMTDVDCVRMIPMRLALRIVDIS
                     SPTLKTRIIASSHQAVEGPTEGISKEELKAELRMLMRQYRRLRPAQEDDFALNELSFV
                     AEQFQSLFGMVNRVGWLVAIFSLLVGGFGISNIMYVSVEERTRIIGIKKALGARKRVI
                     TMQFLAEAATLSTLGGVAGIALVWIVTLLIPDTLFQIYLSPANVLKGIAIALVIGIIS
                     GVAPARYAASLHPVDAING"
     gene            complement(2659..>2991)
                     /locus_tag="PLI69_05660"
     CDS             complement(2659..>2991)
                     /locus_tag="PLI69_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HOG42006.1"
                     /translation="LLNGEPADALSTLIHQDHAYDFGRKICERLKELIPRQQFDIAIQ
                     AAIGSKIIARETVRQLRKDVTAKCYGGDISRKRKLLEKQKAGKKRMRQIGSVEVPQSA
                     FMAVLKLD"
BASE COUNT          732 a          727 c          811 g          721 t
ORIGIN      
        1 attactggat attcagcaga gggagaagga gcgtaccggc ataccttcgc tccgtatcag
       61 ctacaacaat gtcttcggat atttcctcga agtgagaaat acccataaag agcgcgtacc
      121 ctctgattgg atccgcaagc agacactggt aaatgcggag cgttatatca cgccggagct
      181 caaacaatac gaagagaaga tactcggggc tgaggagaag attctcgccc tcgagagtga
      241 gatatatgga gctttgcttg cctccataca agccgaagtt gccctcattc agcgcaatgc
      301 ttcggtagtc tcccgcctgg acgtactcgc gggttttgcc cggctggcgc tggacaaccg
      361 atactgccgc ccgcaggtgg acgattcgtt ggagattgag atcgaaggag gccgccaccc
      421 cgtgatagag acccgtatgg ctcccggcga ggagtatgta cccaacgatc tataccttaa
      481 caatgactcg cagcagataa taatattaac cggccccaat atgtccggaa aatcggctct
      541 cctgcgacaa acggcgctga tagttgtaat ggcccagata gggtcgttcg tccccgcaaa
      601 tcgcgtaaaa atgggcatta cggataaaat attcaccaga gtcggagcct ctgacaatat
      661 tgcccgcgga gagtccacct tcatggtgga aatgatcgag actgccacca tcttgaataa
      721 tctttcccaa aggtcgctgg tgttgctcga cgagatagga cgaggtacca gtacttatga
      781 cgggatgtcc atcgcctggg ccatagtgga gcatttgcat ggcggtaccc agagggcaaa
      841 gaccctcttc gccacccatt atcatgagct taacgaccta gaggcgcttc acgagcgtgt
      901 attcaactat catatctccg tcagggagag tgaaggtaag attatctttc tgcgcaagct
      961 ctgcagggga ggtgtcgccc atagtttcgg catccacgta gcccgtctgg cagggatgcc
     1021 cgatgatgtg gtaaaacggg cagaggcacg cctttccgag ctcgagagag agggaggcgc
     1081 ggccggtgga actctcgcag aggcgccttc cgacagtagc tctatcggca ctcaggaaga
     1141 gacaccttcc gacagtagct ctatcggcac tcgcagcagc gacattaatg taggctccgc
     1201 aactttttca agaaaaacca aaaaatccgc ccgctccact ccggatacgg gactacagct
     1261 caccctatac caattggatg atccgctgtt gctagatatc aaggaggagc tccaaaatat
     1321 tgatatcaac aaaatgtctc ccttagacgc attcgacacc ataaggaggc taaaaaagaa
     1381 aataggacta taaaaagagc attgtagaag gatttgtgat gagagagttg ttggcggata
     1441 aattcaggac caccctttca cttgcgggtg taagcatagg cattttttcg attgtggcgg
     1501 tctttaccgt ggtggattcc ctaaaagaga gcgtaaatga aggtttccgt aaattcggaa
     1561 gcgacgtggt catggtagag agggtgcctc tcgaacctga ctttgatcag gatgggcgac
     1621 agaagtggtg gcaatatgag agccgtccgc agataacctg gcaggagtat ctgtttctga
     1681 aggaaaagag tactacggca ggtcgcatgc tcttttcggc ctcatttgag atggagtgtg
     1741 cggccggtcg tgaaagttat aaggagggcg ccttcgtcgc ggtagagggc gattggagga
     1801 tgatggtgca ggagaatatt ctttacggac gcggttttac ttccggagaa ctgtcgggcg
     1861 gtgcggctgt taccattatc ggctcggatg tcgcacaaaa gctttttccc gatatcacgg
     1921 accctgtcgg tcgtatgata acggtgggag gcactccgct tagagtgata ggcctctttg
     1981 ccccgtccgg aaataatctg gtcagcatga cggatgtgga ttgtgtaaga atgatcccga
     2041 tgcgacttgc tcttcggatt gtggacatct cctctccgac tctcaaaact cgtatcatag
     2101 cctcatcaca ccaggccgta gaggggccta cggaggggat ttccaaagag gaactgaagg
     2161 cggaactgcg gatgctgatg cgccaatacc gtaggttgcg gcccgctcag gaagatgatt
     2221 ttgccctaaa cgaactctct tttgttgcag aacaatttca atccctattt ggaatggtaa
     2281 acagggtcgg ctggctggtg gctatttttt cgttgcttgt cggcggattt gggatatcaa
     2341 atataatgta tgtttccgtg gaggagcgca cccgtatcat aggtattaaa aaggctttgg
     2401 gagctagaaa gagggtcatt acgatgcagt ttctggctga agcggccaca ttgtccacac
     2461 tcggcggagt agccggcata gcgctggtct ggattgtcac acttttaatt ccggataccc
     2521 tttttcagat atatctctca ccggccaatg ttcttaaagg gatagcaata gcacttgtaa
     2581 tagggattat ctccggtgtc gctcccgccc ggtatgccgc ttccctacat cccgtagatg
     2641 ctattaacgg ttgatctctt aatcgagctt gagaacggcc ataaaggcac tttgaggcac
     2701 ctcaaccgag ccgatctgac gcatgcgctt tttacccgcc ttctgctttt caaggagttt
     2761 gcgttttctg gagatgtcgc ctccataaca tttggcggtc acgtccttgc gcaactgacg
     2821 aaccgtctcc ctggctatga tcttggaacc gatggccgcc tggatggcga tatcaaactg
     2881 ttgacgcggg atcagctcct tcaatctttc gcagatcttc ctgccgaaat cgtatgcatg
     2941 gtcctgatga atcagagtgg agagcgcatc cgccggctct ccgttaagga g
//
LOCUS       DAOSIE010000186         2974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_28911_length_2974_cov_4.209318, whole genome shotgun sequence.
ACCESSION   DAOSIE010000186 DAOSIE010000000
VERSION     DAOSIE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28911_length_2974_cov_4.209318"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2115
                     /gene="topA"
                     /locus_tag="PLI69_05665"
     CDS             <1..2115
                     /gene="topA"
                     /locus_tag="PLI69_05665"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOG42007.1"
                     /translation="KKKVVSELTAAAKKADMVWLASDEDREGEAIAWHLQQTLEIPSE
                     KSRRIVFHEITKNAIVDAIDNPREIDSNLVMAQQARRVLDRLVGFKLSPVLWKKIQPK
                     LSAGRVQSVAVRLIVEREREISSFEAQRYFRVEGIFIPEGSNARIKGILDKKFPTFEE
                     AESFLERCKGCSFDISSIEKKEITRTPAPPFTTSVLQQEASRKLGLSVSQTMVIAQRL
                     YESGLITYMRTDSTNLSSLALATAKESITSMYGVEYSQQRNYKTKSKGAQEAHEAIRP
                     TYISNTSIEGTANEKKLYTLIWKRTIASQMADARLEKTDVTISGDSISEKFIVQGEQI
                     IFDGFLKVYIEGRDDEDESEKAALLPSLNIGQRMVDESIVATEKFTQRPPRYSEASLV
                     KKLEELGIGRPSTYAPTISTILKRGYVIKSDKTGTPRDYREITFSKGNITSTVKKEIA
                     GREKNRLCPENIGMLVTDYLSANFPDILDYGFTAKVEADFDNIAKGLLVWNEVIGDFY
                     GPFHEKVTNALEERTYTNVEKQLGIDPATGKPVIARMGRFGPLVQRGSNDDPEKQFVS
                     LKKGQLIEDITLEDALQLLKLPRTVGEHEGKTITTAIGRFGPYIKVGSAYISLPKELD
                     PFTVTMDDAVKLIAEHERKESEKYIAEFPEHKIQILNGRYGPYIKQGKNNYKIPRGTD
                     PSSLNVTDCLTIIEKEEAKKSK"
     gene            2175..2666
                     /locus_tag="PLI69_05670"
     CDS             2175..2666
                     /locus_tag="PLI69_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOG42008.1"
                     /translation="MSNYQIDQIDQKILSFLVNNARMPFLEIARECGVSGAAIHQRVK
                     KMESMGIITGSTLLVKAKALGLNVCAFVGVSLAQANSYPQVVEALKKMSEVVECHFIT
                     GNHALLLKIYCFDHDHLMEILVNTIQNIPSVMKTETWVSLDQAIERQVWVKDYPNASS
                     PAK"
     gene            complement(2641..>2974)
                     /locus_tag="PLI69_05675"
     CDS             complement(2641..>2974)
                     /locus_tag="PLI69_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOG42009.1"
                     /translation="RSMFRKMEECKALVPVTLPVESLREIGPDGTSTAVDRSRYRLVQ
                     TPQIFHSELLKEAYSQPYNPLFTDDASVVEYMGHRVSLCEGLSSNIKITTPEDLALAE
                     AILRETRR"
BASE COUNT          905 a          668 c          686 g          715 t
ORIGIN      
        1 aagaagaaag tggtttccga actgaccgct gcagcaaaga aagccgatat ggtgtggctc
       61 gcctcggatg aagaccgcga aggagaggct attgcctggc acctacaaca aacattggag
      121 atcccctcag aaaaaagccg tcgtattgtc tttcacgaga ttacaaagaa tgccatagtg
      181 gatgctatag acaatccccg tgaaatcgac tccaatctgg tcatggctca acaggcaagg
      241 agggtgcttg atcgtctggt gggattcaag ctctcacccg ttctttggaa aaaaattcaa
      301 ccaaagctct ctgcagggcg cgtacaatcg gtagcagtga gattgattgt agagagagaa
      361 agagaaatct cttcatttga agcacagcgc tatttcaggg tagagggtat atttatcccc
      421 gagggatcta atgcacgcat taaaggtatt cttgacaaaa aattccccac ttttgaggag
      481 gctgagtcct tcctagaaag gtgtaaagga tgctcttttg acatttcttc catagagaaa
      541 aaagagataa cacgtactcc ggcacctccc tttaccacct cggttctcca gcaggaggca
      601 tccagaaaac tggggttatc ggtaagtcag actatggtaa tagctcaacg actctacgag
      661 agcggtctga tcacctatat gcgtaccgac tcaaccaatc tctcatcctt ggcacttgcc
      721 actgcaaaag agagtatcac ttcaatgtat ggagttgaat attcccaaca gcgcaactat
      781 aagacaaaaa gcaagggtgc acaggaagca catgaggcca tccgtccaac ctatatatcc
      841 aacacctcga tagagggcac cgctaacgag aagaaactct atacgctaat ctggaagagg
      901 acaattgctt cacagatggc cgacgcgcgt ctggaaaaaa cagatgttac catctccgga
      961 gacagcatta gcgaaaagtt tattgtacag ggagaacaaa ttatctttga cggcttttta
     1021 aaggtctata tcgagggacg tgacgatgag gatgaaagcg aaaaagcagc tcttttaccc
     1081 tccctcaaca ttggacagag gatggtggac gaaagcatcg tcgctaccga aaaatttacc
     1141 cagaggccac cgcgctatag tgaggccagc ttggttaaaa aactggaaga actcggcata
     1201 gggcgccctt cgacctacgc cccaaccatc tctaccattc ttaagagagg atatgtcata
     1261 aaaagtgata aaaccggtac ccccagggat tatcgtgaga ttaccttcag taagggcaat
     1321 ataacctcta ccgtcaaaaa agaaattgca ggtcgtgaaa agaacaggct ctgccccgaa
     1381 aacataggta tgttggtgac cgactacctt tcagcgaact ttcccgatat tcttgattat
     1441 ggctttacag caaaggtaga ggctgatttt gacaatatag caaaaggtct gctggtatgg
     1501 aatgaggtaa ttggcgactt ttacggacct tttcacgaga aggtcacaaa tgcactggaa
     1561 gagagaacct acacaaacgt ggaaaagcaa cttggaatcg atcctgctac ggggaaaccg
     1621 gttattgcac gtatgggacg tttcggtcct ctggtacaga gagggagcaa tgatgatcct
     1681 gagaaacagt ttgtaagtct aaagaagggc cagttgatag aggacattac tcttgaggat
     1741 gcacttcaac tcctaaaact cccccgtacc gtgggagagc atgaaggtaa aaccatcacg
     1801 actgctatag gtcgcttcgg accctatatt aaggtgggct ctgcatatat ctctctgcct
     1861 aaggaacttg acccatttac cgtcactatg gatgatgcgg taaaattgat tgcagagcat
     1921 gaacgaaagg agagtgagaa atatattgct gaatttcccg agcataaaat ccagatctta
     1981 aacggtcgtt acggacccta cataaagcag ggtaaaaaca actataaaat acctcgaggt
     2041 accgatccgt cttctctaaa tgtcactgat tgtctgacta ttatagagaa agaagaggca
     2101 aaaaaatcaa aataggttat aatttgtaac caacttgcgg tttttttcat atctttacgc
     2161 ttaaaataac atttatgagc aattatcaga ttgatcagat tgatcagaaa atcctctcat
     2221 ttctcgtaaa taacgcgaga atgccctttc ttgaaatagc acgcgagtgc ggagtgtccg
     2281 gagcagccat acaccagaga gtaaagaaaa tggaaagtat gggcatcatt acaggttcca
     2341 cgcttttggt aaaggcaaag gctctcggac tcaatgtttg tgcctttgtg ggagtctccc
     2401 tggctcaggc caacagttat ccgcaagttg tcgaggctct caaaaagatg tcggaagtgg
     2461 tagagtgcca ttttataacg ggtaaccatg ctcttctact caaaatatac tgcttcgacc
     2521 acgaccacct tatggaaatt ctggtcaata ccatacaaaa tattcccagt gtgatgaaga
     2581 ccgaaacctg ggtctctctc gaccaggcaa tagaacgcca ggtttgggta aaggattatc
     2641 ctaacgcctc gtctcccgca aaatagcctc ggctaatgcg aggtcttccg gagtggtaat
     2701 cttgatattg ctgctaaggc cctcgcatag cgagactctg tgtcccatat attccaccac
     2761 tgaggcatca tctgtaaata aaggattata gggctgagaa taggcctcct tgagcaattc
     2821 cgaatgaaat atctggggag tctgtaccag acgataacga gaacggtcta ccgccgtaga
     2881 agttccgtca ggtccaattt cgcgcagcga ctctacaggc agggtcaccg gtaccagcgc
     2941 tttgcactcc tccatttttc taaacattga ccta
//
LOCUS       DAOSIE010000187         2935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_29386_length_2935_cov_3.737153, whole genome shotgun sequence.
ACCESSION   DAOSIE010000187 DAOSIE010000000
VERSION     DAOSIE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29386_length_2935_cov_3.737153"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..541)
                     /locus_tag="PLI69_05680"
     CDS             complement(140..541)
                     /locus_tag="PLI69_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42010.1"
                     /translation="MKKIGLLFAFTAIMTLFASCENVEEKVTAVADSFLTSYYQMEYE
                     DAAEYCTDEFAEVLLQAVATKPEVSDLIEELLIAATKATTYTILDVDTKSVEGEATVL
                     FELIPYKSEVTLERKLIMVKSDGEWKVSDFK"
     gene            complement(563..>2935)
                     /locus_tag="PLI69_05685"
     CDS             complement(563..>2935)
                     /locus_tag="PLI69_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOG42011.1"
                     /translation="QLTDEMRLISMFEDTFPSVGFIDTKPFLLPLNVPSTYIDLVSLS
                     KLRISLDTLRKILSFFKGCKDDLYPNLRSLSEKVIYYPEVARRIDSLIDKNGEMRDDA
                     SEQLHTIRRSLKEKEGAISKRISHLLKKAQSEGIVDEDASVSVRDGKILIPVPVANKK
                     RIQGFVYDESSSGKTAFIEPMEVVELNNRVRQLKFEEQREILKILVEFTDFLRPYLEE
                     LIAASDYIGEIDFIRAKANVALDMIVGMPIISQTGELALRKARHPLLEKALRKEKKEI
                     VPLTLTLTREKHILLISGPNAGGKSVCLKTVGLLQYMFQWGMLIPTSEISEMMIFDNI
                     FIDIGDDQSLDNDLSTYSSHLVNIRKILEKATDNSLVLIDEFGSGTEPTAGGAIAEAV
                     LARLEQIGAYGVITTHYTNLKLYANNSSGVSNGAMLFDVAGITPLFKLEQGLPGNSFA
                     FELARNIGLPETIVKDAEERAGSGFVSIERNLRQIARSKRILDEKLARIKSTDKTLES
                     LTDKYHKELGDIKQLRKEIIDEARAEAREIVERSNRKVEQTIREIKEAQAEKEKTREI
                     RKDLEQFRQEMDNSAESELEQRITRKMEQIVARKEREKERKERRKSEAQRQEEAKARA
                     AASKERIISDKPLQVGDKVRIKGSELVGEITQITDKTIAVAVGNIISKMAPSKVERIS
                     SQQYKESTRFSKPRSIVTSTSISERRLNFKPSIDIRGERLDSALDIVTRFIDDALMVG
                     LSEVKILHGKGNGVLREEIRKYLKTVPGVNSLRDEALQLGGAGITVVTLD"
BASE COUNT          715 a          762 c          635 g          823 t
ORIGIN      
        1 agctacggct gcatacggtc acttcggacg agatccattc gtaaaggaga tggaacttat
       61 ccgtaatggt aagaaggtaa aggaaaaagt gcagtttttc ggatgggaaa agctcgatga
      121 aatagaacgc tgtaaagccc tatttaaaat ctgaaacctt ccactcgcca tctgacttaa
      181 ccataatgag ctttctttcc agtgtcactt cggacttgta gggaataagc tcaaacaaga
      241 ctgttgcctc accttccact gacttcgtat ccacatccag aatggtatag gtagtagctt
      301 tggtggcagc aatcaaaagt tcttcgataa ggtccgaaac ctcaggcttt gtggctacgg
      361 cttgaagaag cacttcagca aactcatcgg tacaatattc ggctgcatcc tcatattcca
      421 tctgataata ggatgtcaaa aagctgtcgg caacggctgt aaccttttct tcaacatttt
      481 cgcaggatgc gaagagtgtc attattgcgg taaacgcaaa caatagccca atttttttca
      541 taataattat aattttaaat acttaatcca aggttactac ggtaatgccc gcgcctccca
      601 gttgcagagc ttcatctcga agggagttca ctccgggcac ggtcttgagg tatttgcgta
      661 tctcttcacg gagcactcca ttgcctttgc cgtggagaat ctttacctca gataagccca
      721 ccatcagagc atcgtctata aaacgcgtga ctatatccaa cgcactgtcc aaacgctcgc
      781 cacgtatgtc aatgctgggt ttgaagttga gccgtctctc cgatatcgag gtagaggtaa
      841 ctatggagcg gggcttggaa aagcgggtgc tctccttgta ctgctggctg gagatgcgct
      901 ctaccttgga gggggccatc ttgctgatga tgttgcccac agccacggca atagtcttgt
      961 cggttatctg cgttatctct cccacaagct cggaaccctt gatacgcact ttatctccca
     1021 cctgcagagg tttgtcggaa ataatccgct cttttgaagc tgctgcacgt gcctttgcct
     1081 cttcctgacg ctgggcttcg cttttacgcc tctcctttcg ctctttttcc cgctccttac
     1141 gtgccactat ctgctccatc ttacgggtta tacgctgttc cagctcgctc tcggcactat
     1201 tatccatctc ttgcctgaat tgctccagat ctttgcgaat ttcacgggtt ttctcctttt
     1261 cagcttgcgc ttccttgatc tccctaatgg tttgctccac cttacgattg ctcctctcca
     1321 ctatttcgcg tgcctctgct cttgcctcgt caattatctc ctttcggagc tgcttgatat
     1381 ctccaagctc tttatgatat ttgtccgtga ggctctccaa agtcttgtcg gtgctcttta
     1441 tacgggcaag tttctcatcg agaatgcgtt tgctgcgcgc tatctggcgt aaattgcgct
     1501 caatgctcac aaatccgctg cccgcacgct cttccgcatc cttgacaatt gtctcgggca
     1561 agcctatatt tctagccagc tcaaaggcaa aggagttgcc cggaagaccc tgctccagtt
     1621 taaagagagg tgtaataccg gctacatcga aaagcatggc tccattggag acaccggaac
     1681 tgttgttggc gtaaagcttc agattggtat agtgggtggt aatcactcca taggcaccaa
     1741 tctgctcgag gcgcgccaat acagcctccg caatggctcc tcctgcggtt ggctcggtac
     1801 cgctaccaaa ctcatctatc agcaccagcg aattgtctgt tgccttttca agtattttac
     1861 ggatattgac aaggtgcgaa ctataagtac tcaggtcatt gtccagagat tgatcatctc
     1921 ctatatctat gaaaatattg tcaaatatca tcatttccga tatttcggag gtcggaatca
     1981 acattcccca ctgaaacata tactgcaaca aacccaccgt cttgagacaa acggacttac
     2041 cgccggcatt gggacctgag ataagcaaga tgtgtttttc acgcgtaaga gtgagagtca
     2101 acggcactat ctctttcttt tcctttctca aagccttctc caacagaggg tggcgtgcct
     2161 tgcgcaatgc tagctcacct gtctgcgata ttatcggcat accgactatc atatccaggg
     2221 ccacattagc ctttgcgcgg ataaagtcta tctctccaat atagtctgag gctgctatca
     2281 gctcctccag atagggacgt agaaaatcag taaactccac aagtatcttc agaatctcgc
     2341 gctgctcctc aaacttcaac tggcgtaccc ggttgtttaa ctccaccacc tccatcggct
     2401 ctataaaggc ggtcttaccg ctagaagatt cgtcatagac aaaaccctgg atacgcttct
     2461 tgttggctac aggcacgggt atgaggattt tgccatccct gacggaaacc gaagcatcct
     2521 catccactat cccttcggat tgcgcctttt tgagcaggtg cgaaatgcgt ttggagattg
     2581 caccctcttt ttctttgaga gaacggcgga tggtatgcaa ctgctctgaa gcatcgtcac
     2641 gcatctcacc gttcttgtct attagactat cgatccttcg cgcaacttcc ggataataga
     2701 taaccttttc ggagagactc cgtaaattgg gataaagatc atccttgcac cccttgaaga
     2761 aggagagaat cttgcgcagg gtatcgagtg aaatcctcag cttgctgagg gaaaccagat
     2821 caatataggt agaaggcaca ttcagaggca acaaaaaggg ctttgtatct ataaagccca
     2881 ccgaaggaaa tgtatcctcg aacatcgaga tcaaacgcat ctcatctgtt agttg
//
LOCUS       DAOSIE010000188         2919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_29606_length_2919_cov_3.861034, whole genome shotgun sequence.
ACCESSION   DAOSIE010000188 DAOSIE010000000
VERSION     DAOSIE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29606_length_2919_cov_3.861034"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLI69_05690"
     CDS             <1..517
                     /locus_tag="PLI69_05690"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG42012.1"
                     /translation="RLTDSSGRTVDFRNTILILTSNIGSRKLRDFGGDVGFSTSTTDR
                     DFRRKSIIDKALERQFSPELLNRLDDRIYFNSLTREDIFRIIELELADLADRVGHLGY
                     ELVVDQSARTFVTDQGFDPELGARPLKRAIQRHIEDPLSELIISDGSGSGVLKVTFDE
                     QSGGIAVTHIA"
     gene            complement(501..1349)
                     /locus_tag="PLI69_05695"
     CDS             complement(501..1349)
                     /locus_tag="PLI69_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42013.1"
                     /translation="MIAEFSEFLESFSVRYERCGTLFSFPGRDVTIRLVPLKRLDIAN
                     LVQGVENDAADDLTSVAVNATEKIADGGDRCIYIYEDRWRSGGEMLRKRILAHLGQFK
                     SVFARKCEVKPLKAQEATEFLEKYHTYGTSRCKYRYGLFYEEKLVAVATFSAGRPMRR
                     LVGGEDRILMSYEWVRYASLPDCRIVGGMGRLLEAFVNDVNPDEVMSYSDYEWSHGEA
                     YLQLGFQKVGEKLPMRFYVDRTTFRRISEKKVSADKRFRGETFPKEKYALISNLGSAK
                     YLKQYV"
     gene            complement(1346..2917)
                     /locus_tag="PLI69_05700"
     CDS             complement(1346..2917)
                     /locus_tag="PLI69_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOG42014.1"
                     /translation="MYLQGKMANRHGLIAGATGTGKTVSLQVLAETFSQAGVPCFMAD
                     MKGDLSGISQSGKMNGFIEKRCAEFGIENPFFEGSPTRFYDVFGEQGYSMRTTVSAMG
                     SQLLARLMELNETQAGVLNIVFRIADDKELLLIDLKDMRLMLDFVGKNASQFTTEYGN
                     ISAASIGAIQRAVLAIESQGGDKFFGEPAFDVNDLIATENGRGVMNVLAADKLMHNPK
                     LYSTFLLWLLTELYVQLPEVGDQPLPKLVFFFDEAHMLFQGTSKALIDKIEQVIRLIR
                     SKGVGIYFVTQSPADIPDIVLGQLGNRIQHALRAYTPKDQKVVRAAAQTFRPNPNFKT
                     DEAITELATGEALVSFLDEKGAPSIVERARILFPLSSIGPITEGQRLDINNAAPAEIK
                     AYDKFFDRESAYEILMDHYEKAAKAAEEAMIEAEKEKAEIEKQKQEKKQSSGKKGGIS
                     RTIFGTMIGAAATSFLRSLGTQAAREVVGRRTKSTKSSTSKKSGDTLIDRTAKSATTT
                     ATRKITEEVLKNIFK"
BASE COUNT          736 a          748 c          648 g          787 t
ORIGIN      
        1 aaggcttacc gatagcagtg gccgtacggt ggacttccgc aacactattt tgatcctcac
       61 atccaatatt ggaagccgta aattacggga cttcggagga gatgtgggat tctccaccag
      121 cactaccgac agggacttcc ggcgcaaatc cattatagat aaagctctgg agcgtcaatt
      181 ctctccggaa cttctaaatc gtctggacga taggatctat ttcaattccc tcaccagaga
      241 ggatattttc cgtataatag agctcgagtt ggctgatctg gctgacagag tcgggcacct
      301 tggatatgaa cttgtggtag accaaagcgc ccgtacattc gtaaccgatc agggattcga
      361 ccccgaactt ggcgcaagac ctttaaagag ggctattcaa aggcatatcg aagacccgct
      421 gtccgaactt atcataagcg acggatcggg cagtggagtt ttgaaagtta cttttgacga
      481 acaatccggc gggattgcgg tcacacatat tgcttaagat atttagcgct gcccagattg
      541 gatataagcg catatttttc cttcgggaaa gtctctccgc gaaatctctt gtctgcggag
      601 actttttttt ccgaaatcct cctgaatgtg gtgcggtcta cataaaagcg catcggaagc
      661 ttttcaccta ccttttggaa accgagttga agataggctt ccccatggga ccattcgtaa
      721 tcagaatatg acatcacctc atcaggattg acatcattga cgaaggcctc caggagtcgt
      781 cccatacctc ctacaatacg acagtcgggc aatgaagcat acctgaccca ttcgtaactc
      841 ataaggatac gatcctcccc tcctacaaga cgtctcatag gcctgcctgc ggaaaatgtg
      901 gccacggcca ctaacttttc ttcgtaaaaa agcccgtaac gatacttgca tcttgaggtg
      961 ccataagtgt gatatttttc aagaaattcc gtcgcctcct gcgctttcag cggttttact
     1021 tcacatttcc tggcgaaaac ggatttaaat tggcccagat gggcaagaat ccgcttacga
     1081 agcatttccc ctccgctacg ccagcgatct tcgtaaatat aaatgcatct gtcgccacca
     1141 tctgcgatct tttccgtagc attcacggca acactcgtca aatcatccgc cgcatcattc
     1201 tccacaccct gaacgagatt agcaatatcg agtctcttga gcggcaccag ccgaatggtt
     1261 acatccctgc cgggaaatga aaagagagtc ccgcagcgct cataacgcac tgagaaactc
     1321 tccagaaatt ctgaaaattc tgcaatcact tgaagatgtt tttaagcacc tcttcggtta
     1381 tctttctggt ggctgtagta gtagcgctct tagctgttct gtcgattaga gtatcaccac
     1441 tctttttaga tgtcgagctt ttagtggatt tcgtcctcct gcctactact tctcttgctg
     1501 cctgagtacc cagactgcgc aggaaacttg tggcagcagc acctatcata gtaccgaaaa
     1561 tagttctgga gatacctccc tttttccctg aggattgctt cttctcctgt ttctgttttt
     1621 caatctccgc tttctccttc tcagcctcta tcattgcctc ctcagccgcc ttggcagcct
     1681 tttcgtaatg atccatcaaa atttcataag cactttcacg atcaaaaaat ttatcataag
     1741 ccttgatttc cgcaggtgca gcattgttta tatcgagtcg ctgcccctct gtaatgggtc
     1801 cgatggagct tagaggaaat aagatcctgg ctctttccac tatggaaggg gctccttttt
     1861 cgtcgaggaa ggagacgagt gcctctccgg tagcaagctc cgtgatagcc tcatctgttt
     1921 tgaagtttgg attgggtcgg aaggtctgtg ctgcagcgcg taccaccttc tggtctttag
     1981 gggtataagc ccgaagagcg tgctgtatgc ggttgccaag ctgtcctaag acaatatcgg
     2041 ggatatcggc aggtgattga gtgacaaaat aaatacccac ccccttggag cggatcagac
     2101 gtataacctg ttcgatctta tcaataagag ccttggatgt gccttgaaat agcatgtgtg
     2161 cctcatcaaa gaagaagacc agcttgggaa gaggttggtc tcctacctcg ggaagttgca
     2221 cataaagttc agtaagcaac cacaaaagaa aagtagagta aagttttgga ttatgcatca
     2281 gtttatcggc agcaaggaca ttcatcactc cccgaccgtt ctcggtagca atcaggtcat
     2341 tgacgtcaaa tgccggttcg ccgaaaaatt tatctccacc ctggctctca atggcgagca
     2401 ctgctctctg tatggccccg atgctggctg ctgagatgtt accatattcg gtagtaaact
     2461 gcgaagcatt cttacccaca aaatcgagca tcaaacgcat atctttcaga tctatcagca
     2521 gcagttcctt gtcatccgct atacggaaaa caatgttgag tactccggcc tgggtctcgt
     2581 ttagctccat caggcgcgca aggagttggg aacccattgc cgaaacggtg gtgcgcattg
     2641 agtacccctg ctctccaaaa acatcataga atctagtggg agagccttca aagaatggat
     2701 tttcaatacc gaattccgcg catcgcttct caataaaacc gttcattttt ccagactggc
     2761 tgatacctga aaggtctcct ttcatatctg ccataaaaca gggcactccc gcctgggaaa
     2821 atgtctccgc cagcacctga agtgaaacgg tttttcccgt gccggttgca cctgctatca
     2881 gtccgtgcct gtttgccatc ttgccctgaa gataaatag
//
LOCUS       DAOSIE010000189         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_29738_length_2908_cov_3.173151, whole genome shotgun sequence.
ACCESSION   DAOSIE010000189 DAOSIE010000000
VERSION     DAOSIE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29738_length_2908_cov_3.173151"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLI69_05705"
     CDS             complement(<1..373)
                     /locus_tag="PLI69_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase subunit B"
                     /protein_id="HOG42015.1"
                     /translation="MIFQGKQKIWSDYQKEIPDDHWFYVRSCIRQGFFPGSEVAFLDI
                     CRNKLGKDFFETEHHTTCGGIAYHAEALPQETMMTIIARQFALMGEHGYKNYVCSCIT
                     SFGLYTEVLEDWRTFPDLEEKI"
     gene            complement(392..1087)
                     /locus_tag="PLI69_05710"
     CDS             complement(392..1087)
                     /locus_tag="PLI69_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG42016.1"
                     /translation="MGKLYEQLIRDYRIKEGLKACINCGTCTAICPAAEFYKYNPKNI
                     VNIVQSQDDAQIEELLKGEQIWYCGECMSCVTRCPRRNAPGLVVMALRSLSIDLGYFV
                     ESEKGRQMLPLTRRMQSNILNYGYCVYPRTFLYKDHPEYGLTGKWLNENMEEVYERLG
                     ANLDGKGPGILRKIPDESLAELKAIFDETGATRRMEIIEEASKLKAKEMGIPYDEYER
                     KAFEDTEKDHLNH"
     gene            complement(1232..>2908)
                     /locus_tag="PLI69_05715"
     CDS             complement(1232..>2908)
                     /locus_tag="PLI69_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG42017.1"
                     /translation="SLGIVPYVKQIDTLAGEFPAQSNYLYLTYNGQEHDVEYKNDRRS
                     IIVLGSGAYRIGSSVEFDWCSVNALQTIRKLGWRGVMINYNPETVSTDYDICDRLYFD
                     ELTFERVLDIYELESPHGIILSVGGQIPNNLALRLDENGVNILGTSAASIDRAEDRHK
                     FSSMLDSIKVDQPKWQELTTLDDIHIFVESVGFPVLVRPSYVLSGAAMNVCSNEGELD
                     RFLSLAANVSKKHPVVVSEFIENAKEIEFDAVANRGEVIAYAISEHIEFAGVHSGDAT
                     IQFPPQKLYVETARRIKKIARTIASELNISGPFNIQFLAKENDIKVIECNLRASRSFP
                     FVSKVLKINFIELATRVMVGENPVPPSKSAFDLDYVGIKASQFSFSRLQKADPVLGVD
                     MASTGEVGCIGDDTNEAILKSMLSVGYTIPKKNILLSTGDARQKAEMLSASRMLVQHG
                     YKLFATDGTYKFFVQNNVPVTRVLWPSESGNERSALEMIQNKEIDFVVNIPKNLTESE
                     LSNGYKIRRAAVDFNIPLITNTRLATAFIISFCNLPIEEIQIKSWDEYAS"
BASE COUNT          729 a          661 c          681 g          837 t
ORIGIN      
        1 gaattttttc ctcaagatcc ggaaaagtcc tccagtcctc caacacttcg gtatagagac
       61 caaatgatgt gatgcaggaa catacgtagt ttttgtagcc atgttcgccc atcaaagcaa
      121 attggcgggc tataattgtc atcatggtct cctgcggcag agcttcggca tggtatgcaa
      181 tgcctccgca agtggtatgg tgctccgtct cgaaaaaatc tttgccgagt ttgttacggc
      241 atatgtcgag aaatgccact tccgatcccg gaaaaaatcc ttgacgtatg cagcttctta
      301 cataaaacca atggtcatca ggaatttctt tctgataatc cgaccatatt ttctgttttc
      361 cctgaaatat cattttgatg ctgttttaaa gttaatggtt gagatggtcc ttttcggtat
      421 cctcaaatgc cttcctttca tattcgtcgt aggggatgcc catctctttt gctttaagtt
      481 tagatgcctc ttcgataatt tccatacggc gcgtagcacc ggtctcatca aatattgcct
      541 tgagttcggc gagggattcg tcggggatct ttctaagaat tccgggtcct ttgccgtcga
      601 gattcgcccc gagacgttca tagacctcct ccatgttttc gtttagccat ttaccggtca
      661 gaccatattc ggggtggtct ttgtagagaa atgtgcgagg atagacacag tagccgtagt
      721 ttaggatatt gctctgcata cggcgcgtga gcgggagcat ctgtcgtccc ttttcgcttt
      781 ctacaaaata gcccaaatca atggagagcg atctcagcgc cattaccaca aggccgggag
      841 cattgcgcct cggacaacgt gttacgcacg acatacattc gccgcagtac cagatctgtt
      901 cgcctttgag caattcctca atctgagcgt catcctggct ctgaacgatg ttgactatgt
      961 tttttggatt gtatttgtag aattcggcag cagggcagat ggccgtacag gtgccgcagt
     1021 ttatacaggc tttcagcccc tctttgatgc ggtaatcccg gattaattgt tcgtatagtt
     1081 tgcccataca atttaagttt tagtcgaaca aagttaacaa aaaacctata caaaccacag
     1141 attatgaaat aaattgacag aggctgtgtt gccaaaagtg aatccgattg acaaagatca
     1201 gcccaatatt actaagaaaa attcttgcct gttatgatgc atattcgtcc caactcttaa
     1261 tctggatctc ctcaatgggt agattgcaga aagagatgat gaacgcagta gccagtcggg
     1321 tattggtaat cagcggaatg ttgaagtcca ctgccgcgcg acgtatttta taaccgttac
     1381 tcaattctga ttcggtaaga ttcttgggaa tgttgaccac aaagtctatt tccttgttct
     1441 gaatcatctc cagtgcactg cgctcatttc cgctctcact aggccagaga actctggtga
     1501 cgggaacatt attttgcaca aagaatttgt aggtaccgtc cgtcgcaaag agtttgtagc
     1561 cgtgttgtac cagcatacgg gatgcgctaa gcatttcggc cttttgacgc gcgtctcctg
     1621 tggagagcag gatgttcttt ttaggaatgg tgtaaccaac ggagagcatc gatttcagta
     1681 tcgcttcgtt cgtgtcatca ccgatacaac caacttcacc tgtggatgcc atatctacgc
     1741 ccagcacggg gtcagctttc tgcaaacgtg agaatgaaaa ttgggaggcc ttgataccga
     1801 cataatcgag gtcaaatgca ctcttggagg gtggtacggg attctctcct accataaccc
     1861 gcgttgcaag ttcaataaag ttgatcttta gaactttaga aacaaatggg aaactcctcg
     1921 atgcgcggag gttacattca attaccttga tatcgttctc ttttgcaaga aactggatat
     1981 tgaatggacc ggagatattg agttccgatg caatagtgcg ggcaatcttc ttaatccttc
     2041 ttgcagtctc tacatagagt ttctgaggag gaaattgtat ggttgcatca ccggagtgta
     2101 ctccggcaaa ttctatatgt tctgatattg cgtaggctat cacttcgccg cgattggcta
     2161 cagcatcaaa ttcgatttcc ttggcatttt ctataaattc gctaaccact acaggatgct
     2221 tcttggaaac attggctgcc agggagagga atctgtccag ttcaccttca ttggagcaaa
     2281 cattcattgc cgcacctgaa agcacatagg aggggcgcac aaggacgggg aatccgactg
     2341 attccacaaa tatgtgaatg tcgtcgaggg tggtcagctc ctgccattta ggctggtcaa
     2401 ctttgatgga atcgagcatt gaggagaatt tatgcctgtc ttcggctcta tcaatgcttg
     2461 ctgcggaggt tcccaggatg ttgacgccat tctcgtcaag acgtagggcc agattgtttg
     2521 gaatctgtcc tccgacggag agaataatgc cgtgcggcga ttccagttcg tatatatcca
     2581 gtaccctttc gaatgtcagc tcgtcgaaat agagacgatc gcatatatcg tagtcggtag
     2641 agacggtttc aggattataa ttgatcataa cgcctctcca acccaattta cggatagttt
     2701 ggagcgcatt tacgctgcac cagtcaaatt ctacggagct gccgatgcgg taagcgccgg
     2761 aaccgagcac gataattgac ctgcggtcat tcttatattc tacatcatgt tcctgcccat
     2821 tgtaggtgag gtagagatag ttgctctgtg ccggaaactc gcccgccaga gtatcgattt
     2881 gttttacata gggtacaatg ccgagcga
//
LOCUS       DAOSIE010000190         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_29761_length_2906_cov_2.932655, whole genome shotgun sequence.
ACCESSION   DAOSIE010000190 DAOSIE010000000
VERSION     DAOSIE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29761_length_2906_cov_2.932655"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PLI69_05720"
     CDS             complement(<1..1001)
                     /locus_tag="PLI69_05720"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOG42018.1"
                     /translation="MLKESSTGRFVRLLSNVAVLMIFTALSVACSENSTTEEVSEEIQ
                     IMAKEVITDRFGIPRGEYRVVEGKVSSKDYFSTVLNRYGVSHTKVNALVNKSRGIFDA
                     KTFKSGQPYYVYLPVEGDVPDYFVYQKDNLSALVFDLRDTLGVSLVQKQMDVEQQYVE
                     VTITSSLWNDIQEAGASPLLAIKLSEIYAWSVDFFGLQKGDSFKVLYNEHSCEGEVTD
                     IGEVWFSTYTSGGKSIECIYFEEEGTSNRYWNAEGESMRKSFLKSPLKYFSRISSGFT
                     YARRHPITRVVRPHTGVDYAAPTGTPVLTIGDGVVIEKGYKGGGGHTVKIRHNSVYTT
                     "
     gene            1154..1600
                     /locus_tag="PLI69_05725"
     CDS             1154..1600
                     /locus_tag="PLI69_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42019.1"
                     /translation="MAKFIGEYKARVDDKGRLIFPSAFKSAMGSGADRRFVIKNSLFS
                     ECLEMYAYDEWEKESELVRSRLNFFKREHAEFWREYMRGSHVVEPDEKLGRISIPKDK
                     LESIGVLKDVIFFGNNHKIEVWAKEKFEAQQLDGDAYVALAEKILG"
     gene            1619..2521
                     /gene="rsmH"
                     /locus_tag="PLI69_05730"
     CDS             1619..2521
                     /gene="rsmH"
                     /locus_tag="PLI69_05730"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOG42020.1"
                     /translation="MSTYHIPVLLDESVSALDVRENGVYVDATFGGGGHSRAILQRLG
                     SKGRLMVFDKDYEAFANLPDDDRVIAVNNNFRFLHNYVRYHGFDSVDGIIADLGVSSH
                     QFDTSERGFSFRFDAPLDMRMNLDARKSARDIVNSYTLEELDRVFRIYGEVDESRKVA
                     DLVCRARENSPIETTGDLGRALESVLPKFSEHKYLAKIYQALRIETNDEMRDLEELMK
                     GVIRSLRSGGIVAIISYHSLEDRIVKNFLKSGNASGRIERDLMGHGSSPFELLNKKPI
                     IPEEDEISGNTRARSAKLRVAKRR"
     gene            2524..2856
                     /locus_tag="PLI69_05735"
     CDS             2524..2856
                     /locus_tag="PLI69_05735"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOG42021.1"
                     /translation="MGLRRKIIDKTTSFFSGSFILKRRLDRFVWFFIYIFILLVLFIS
                     WNLYIESRFVRIEKNSAIIKDLEISHQQHSLDLMGIDNRIKVEKILQGFGSRLHAPTD
                     PPKRVKGE"
BASE COUNT          850 a          616 c          660 g          780 t
ORIGIN      
        1 gtagtataaa cggaattgtg gcgaatttta acggtatgtc caccgcctcc tttataaccc
       61 ttttcgatta ccactccatc tcctatcgtt agcaccggag tacctgtagg agctgcataa
      121 tcgacaccag tatgtggcct taccactctg gtaatgggat gtctccttgc gtaggtaaat
      181 cctgaactta tccttgagaa gtactttaaa ggtgatttga gaaaagactt tctcatgctc
      241 tccccttcag cattccaata acgattggat gtaccctcct cttcaaaata gatacattct
      301 atgctcttgc ccccactcgt ataggtggag aaccatactt cgccaatatc agtcacctct
      361 ccttcacaag agtgttcatt atataacact ttgaaagaat cacctttttg caacccgaaa
      421 aagtccacgc tccaggcata tatttcagag agtttgatgg caagaagcgg tgaagctccg
      481 gcttcctgga tatcattcca taaggaagaa gttatggtaa cttcaacata ctgctgctcc
      541 acgtccatct gtttctgcac aagtgatacg ccaagagtat ccctcaggtc gaaaactagt
      601 gcggaaagat tatccttctg gtagacaaaa tagtcgggta catccccctc tacgggtagg
      661 taaacataat aaggctgccc ggatttgaaa gtttttgcat caaatatacc ccgggatttg
      721 tttacaagag catttacctt ggtatgggac actccgtaac gattcagcac ggtggaaaaa
      781 tagtctttac tactcacctt accttctact accctatatt ctcccctcgg tataccgaaa
      841 cgatcggtaa taacctcctt agccattatc tgaatctcct cagaaacctc ttcggttgta
      901 ctgttttcac tgcatgccac cgacaaagca gtaaaaatca tcaacactgc cacattggac
      961 agcaacctga caaatctacc tgtagaactc tctttaagca tctttttttt tctgaatttt
     1021 cctgcaagtt aaccaaattt catcactctg ttgatatttt tgtggaaaaa tttggaaaaa
     1081 tttgtaaata agtggaaaac tattattact tttgcactac acatcatata aaacactata
     1141 ctatcacatt tgaatggcaa agttcatcgg cgaatacaaa gcaagagttg acgacaaggg
     1201 gagattaatt ttcccctctg ccttcaaatc cgctatgggt agcggagccg atcgtcgttt
     1261 tgtcataaaa aattccctgt tctccgagtg tctcgagatg tacgcttatg acgagtggga
     1321 gaaggagtcc gaactggtta ggagccggct caacttcttc aagcgcgaac acgctgaatt
     1381 ctggagagaa tatatgcgtg gcagtcatgt cgtggagcct gatgagaaac tcggtagaat
     1441 ctccatcccc aaggataagc tcgaatctat cggcgtcctg aaggatgtga tattcttcgg
     1501 caacaatcac aagatcgagg tctgggctaa ggagaaattc gaagcccagc aactcgatgg
     1561 tgatgcctat gttgcacttg ccgaaaaaat tctcggctag aaaagaagga ggtgagagat
     1621 gtcaacctac catatacccg tattgcttga tgagagcgta tcggctcttg acgtgaggga
     1681 aaacggagtc tatgtggatg ccacgtttgg aggtggaggc cacagccggg ctatccttca
     1741 acgccttggc agcaaaggtc ggcttatggt atttgataaa gactacgagg cgtttgccaa
     1801 tttgcccgat gatgacagag taatagcggt taacaacaac ttccgatttc tccacaatta
     1861 tgtgagatac catggatttg atagtgtaga tggaattatt gcagacttag gggtctcatc
     1921 ccaccagttt gatacaagtg aacggggttt ttcctttcga tttgacgctc cgctggatat
     1981 gagaatgaac ctggatgcac gtaagagtgc cagagacatt gttaatagtt atacgttgga
     2041 agagttggat agggtgttca ggatctacgg ggaggtagat gagagcagga aggttgcaga
     2101 tcttgtatgc agggccaggg agaactctcc catagagaca accggagatt tgggcagagc
     2161 tttggaaagt gttctgccca agttttcaga acataaatac ctcgcaaaga tctaccaggc
     2221 tcttagaata gagaccaatg atgaaatgcg ggacctggag gagctgatga agggcgttat
     2281 acgttcactc aggagcggag gaatagttgc aataatcagt taccattctc ttgaggatag
     2341 gatagtcaag aatttcctca agtccggtaa tgcaagcggc aggatagaga gagacctgat
     2401 gggacacggc tcttcgcctt tcgaactgct caataaaaag cctatcatcc cggaagagga
     2461 tgaaatttcc ggtaataccc gtgcaaggag tgccaagctc agggtggcta aaaggaggta
     2521 aacatgggac tcagaagaaa aattatagat aaaaccacct cgtttttcag cgggtcattc
     2581 atattgaaaa ggagactcga tagatttgtg tggttcttca tctatatatt catacttctt
     2641 gtgctcttca tcagctggaa tctctacata gagagcaggt ttgtaagaat agagaagaat
     2701 tctgccataa taaaggatct ggagataagc caccagcagc actctcttga tctgatgggc
     2761 attgacaata gaataaaagt agagaagata ctgcaagggt tcggatccag gctccatgca
     2821 cccactgatc cacctaaaag agttaaagga gaataaggtt atgaaagtaa acattggtaa
     2881 ggtaggtatg gcttatgtca tattta
//
LOCUS       DAOSIE010000191         2900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_29840_length_2900_cov_3.335677, whole genome shotgun sequence.
ACCESSION   DAOSIE010000191 DAOSIE010000000
VERSION     DAOSIE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29840_length_2900_cov_3.335677"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PLI69_05740"
     CDS             complement(<1..888)
                     /locus_tag="PLI69_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOG42022.1"
                     /translation="MTKITSTEKKTRKKLAIHWQILIGIGLGVVFGIVMTKFIPYEAW
                     SPYIKWAGDMFLRGLNMIVIPLVFSSIALGVAGMGDSKALGRIAGKTFAFYIVTTLIA
                     ATIGLTLVNIVKPGVGADLKLTENVTDLAGTQVSFIDQIVHIVPANIFESLSTGNLLS
                     IIFFAIIFGFFINMVDQKQQTTLKDILQAIYDVIMKVTLFIIKLAPYGVFAIVANVVG
                     KQAGDTKALLDIAGSLGIFVLIVWGGLLIHGGIVLPTLVRVLGKANPWKHISKVSTAL
                     LTTFSTCSSGAALPFNIRDT"
     gene            complement(904..>2900)
                     /locus_tag="PLI69_05745"
     CDS             complement(904..>2900)
                     /locus_tag="PLI69_05745"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOG42023.1"
                     /translation="LEVPLLKKNDSVRYECGVTTFPPRAPRFAGVAVPIFSLRSEEGF
                     GIGDFTDIRKLVDWATITQQSIIQLLPINDTWSTGTWTDSYPYSGISIMALHPIYINP
                     TLLGKVEDAGKAKKFESERKSLNALDSLDYERVLRLKDGWCRTLFEQDGGAFMEDPGF
                     KEFFEANQEWLLPYAAFCILRDKYDTADFSRWGKYSDYDLKKVNTLWKNVRSGREMRY
                     YVYLQYHLHLQMLDARDYAHSRGIAIKGDIPIGITPQSVEAWSEPHYFNMDAQAGAPP
                     DDFSVKGQNWGFPTYNWKRMGEDGYIWWKRRFAKMAEYFDAYRIDHVLGFFRIWEVPS
                     NQVLGLMGHFNPAMPYSYEDMISRGFDFKYDRHATPYIRYYMLKEMFGDRCPMVQETF
                     LDSNELDVFTLKPEFSNQKLIEAWFEGKEDDGLKDGIMALAGEVLFVEDPNNFGCFHP
                     RISAQYTYSYQALSEEEKSAFNHLYDEFFYKRHNSFWQEAAMRRLPQLITATNMLTCA
                     EDLGMIPACVPPVLEQLKILTLEIQRMPKEVGVIMGNPAYYPYLSVCTTGTHDTSTLR
                     GWWIERYGEDCPRHHCEDIVDSHLNSPAMLTILPLQDWFSLDDTLRVGDPESERINIP
                     ANPKHYWRYRMHITLEELFSKSQFNSHIAHKVILSGRSER"
BASE COUNT          797 a          688 c          656 g          759 t
ORIGIN      
        1 agtatcccgg atattgaagg gaagggccgc acccgaagaa caggtggaaa atgtcgtcag
       61 aagggctgtt gagaccttgg aaatatgttt ccagggatta gccttgccga gtacccttac
      121 aagtgtggga agcactattc ctccatgaat gagcaagcca ccccatacta tcagtacaaa
      181 tattcctagg ctgcccgcaa tatccagcag ggccttcgta tctcccgcct gtttacctac
      241 aacattggct actatggcaa aaactccata gggagcgagc ttgatgataa agagcgtaac
      301 cttcattatc acatcgtata tggcttgcaa tatatctttt agtgtggtct gctgcttttg
      361 gtccaccata ttgatgaaaa aaccgaaaat tatggcaaaa aagatgatgg aaagcagatt
      421 gcccgtactc aacgactcga aaatatttgc aggtactatg tgaacgatct gatcgataaa
      481 cgagacctga gtgcctgcca agtcagtaac attctcggtg agtttaaggt cggctccaac
      541 tccgggtttg actatattga ccagcgtcaa tcctatagta gcagcaatta gcgtagtgac
      601 tatataaaac gcaaatgttt tgcctgcaat acgcccaaga gccttggaat ctcccattcc
      661 ggccaccccg agggcaatgg atgaaaaaac cagaggaatt acaatcatat tcagacctct
      721 taggaacata tcccctgccc attttatgta gggggaccat gcttcgtaag ggatgaattt
      781 tgtcattacg atgccgaaaa cgacaccgag gccgatacct ataaggatct gccagtgaat
      841 ggcaagtttc ttacgggttt tcttttcagt agaagtgatt tttgtcatag ttgtgtgtga
      901 tgattatctt tccgagcgac cgctgaggat cactttatgt gctatgtgtg aattaaattg
      961 cgatttggag aaaagctcct cgagggtaat atgcatccta tatcgccagt aatgcttagg
     1021 atttgcggga atattgatac gctcactctc cggatcccct acgcgtagag tgtcatccag
     1081 ggagaaccaa tcctgaagtg gcagtatggt gagcattgcc ggggagttga ggtggctatc
     1141 cactatatct tcacaatgat gtctgggaca atcttctccg tacctctcta tccaccaccc
     1201 tctgagcgta ctggtatcgt gtgtgccggt ggtacatacc gagagatagg gatagtaagc
     1261 cggattgccc ataataacac ctacctcctt tggcattctt tgaatttcca gagtaagtat
     1321 cttgagttgc tcgagtaccg gcggtacaca agcaggtatc ataccgagat cctcagcgca
     1381 agtgagcata ttggtggccg taatcagttg gggcaatctg cgcatggccg cctcttgcca
     1441 gaatgaattg tgtcttttat agaaaaattc atcatagagg tggttgaatg ccgatttttc
     1501 ctcttcggaa agtgcctgat aactgtaagt atactgagcc gaaattctcg gatggaaaca
     1561 tccgaaatta ttgggatcct ctacgaagag tacctctcct gccagtgcca ttatgccatc
     1621 cttcagccca tcatcctctt tgccttcaaa ccaggcttca ataagttttt ggttggaaaa
     1681 ttccggtttg agagtgaaca catccagttc atttgagtcg agaaatgtct cctgcaccat
     1741 cggacaccta tccccaaaca tttctttaag catatagtag cggatatagg gagtggcgtg
     1801 gcggtcatat ttgaagtcga atccacgcga tatcatatct tcataggagt atggcatggc
     1861 cgggttgaaa tgccccataa ggccgagtac ctggttggat ggcacctccc atatcctgaa
     1921 aaaaccaaga acatggtcta tacgatatgc gtcgaagtat tccgccatct ttgcaaaacg
     1981 tctcttccac cagatgtagc catcctctcc catcctcttc caattgtaag tcgggaaacc
     2041 ccagttctgc cctttaactg aaaaatcatc aggcggagca cctgcctggg catccatatt
     2101 gaaataatgt ggctcactcc acgcttcaac actctgggga gtaatgccta tgggaatgtc
     2161 tccttttatg gcaatacccc tggaatgggc atagtcacgg gcatccagca tctgtaaatg
     2221 gaggtgatac tgcaagtaga cataatatct catttcccgg ccggaacgca cgtttttcca
     2281 aagagtattt acctttttaa gatcataatc ggaatatttt ccccagcggg aaaagtcggc
     2341 agtatcatac ttatccctca aaatgcagaa ggctgcatag ggtaggagcc attcctgatt
     2401 agcctcaaaa aactccttaa atccgggatc ctccataaat gcaccgccat cctgctcgaa
     2461 aagcgtacga caccagccat ccttcagacg cagaacccgt tcataatcga gactatcgag
     2521 agcattgaga ctctttcgct cggattcaaa cttctttgcc ttgccggcat cctctacctt
     2581 cccaagaaga gtcggattga tatatatggg atgcaaagcc attatggaga tacccgaata
     2641 gggataagag tctgtccagg taccggtaga ccaggtatca ttgataggta gcagttggat
     2701 aatactctgc tgggtgatag tggcccaatc caccaacttt cgtatatcgg tgaaatcacc
     2761 tatgccaaaa ccctcttcgc tacgcaggga aaaaatggga actgccactc ctgcaaaacg
     2821 tggagcacgt ggcggaaagg tagtaacacc gcactcataa cgaacggaat cattcttttt
     2881 caaaagggga acctcgagca
//
LOCUS       DAOSIE010000192         2897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_29884_length_2897_cov_2.892329, whole genome shotgun sequence.
ACCESSION   DAOSIE010000192 DAOSIE010000000
VERSION     DAOSIE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29884_length_2897_cov_2.892329"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2897)
                     /locus_tag="PLI69_05750"
     CDS             complement(<1..>2897)
                     /locus_tag="PLI69_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOG42024.1"
                     /translation="SLGAYVSRSTSDPYFSLAEGESRIVELEVSSEMYGDSDCRYEPQ
                     FVIGSVRVEDWYYDTADLFENLTITVTDAQGNAANGAEIDWLGFDSETNCYYFKAPYA
                     SNTAGWNGFWELNVTNTGATEIRVEEIGLSECYQSGGETVLDTTINNMVRGGTVVTVS
                     AGNDGFFGTSTIGTPGTAKDAITVGATDYMMDYRASFSSMGPVNRTSPYIKPDVMAPG
                     VGIISAYPGDQYASMQGTSMSCPAVAGTAALMLSGNSTLKPADVKAALMETAVHIGED
                     GTILPVMQPNNAYGAGRINAYEAVNVTGGLGGMKPSDGILRELIGGTLTQFTVTGNVL
                     PIMAVLWNTTAGEPMAGEEVNVSVWYYACYTYERTPVFDQTIRTDADGYIYCPVDITD
                     IRNGTPMWVQITYGDLRVEDQVTKDTISSGQTPNPSPNVPIFESEDYSVPYSATVPIK
                     YPLLAADGSPYTEVVTFTVENYSKTIVEEVLVPVDGVIGYSLNLTGRPLDETDLYLWI
                     NDRSAGSISVGEEPHIFQEVMPLPERAICPPGMSVNLGLMAWSHHGQKTVSQDFDVYV
                     TTLTETEILSLSPAGALTLSTDQGDMQALLAEIAGMKPVTRTGTVRLTNGIGLLNFTM
                     PENGYMAIVQFVSPNNAMAQYSGEATTVLVYGTLEPWLMHRSTPPVHEDLYSGNLTYI
                     GSYSDWPATWDQDAYASVPSDRATITAYIYNYDPILGIESFIPGQTVYLYTENGVQTS
                     VTDADGYCTFTVDTAGKTRMDYILTTAGISGYGTTWIGGTPFSPNNFCPVRPGLISSE
                     LFAPTSIGALSPNTEMYIVDAVPDGDACKITARSYGPDGTPIHEKGFFVFNRQSAANI
                     WNIEATEAAAVVEFTGTETRLVTPAANGTYEVRYGLLNPTSKNIGKNLYTAFRNPEKS
                     VSYKMPDSVLVGTSVPIAFNVSTSDGAPIVSARVVIAAGAAGEPGRSWFNG"
BASE COUNT          543 a          822 c          893 g          639 t
ORIGIN      
        1 gccattaaac caggaccgcc ccggttctcc tgccgcaccg gccgcaatca cgacgcgtgc
       61 gctgacaatt ggcgccccgt cgctggtcga tacgttgaag gcgatcggca ccgatgtccc
      121 gaccaggacg gagtccggca tcttgtagga gacggacttc tcagggttgc ggaacgcggt
      181 gtaaagattt ttaccgatat tcttgctggt cgggttcagg aggccatacc tgacctcata
      241 ggtcccattc gctgcagggg ttaccagccg ggtctccgtc ccggtgaact ccacgacggc
      301 ggccgcttca gtcgcctcga tattccagat gttcgccgct gattgcctgt tgaagacgaa
      361 gaaacccttc tcgtggatag gcgtaccgtc cggcccgtag gatctggcgg tgatcttgca
      421 ggcatcgcca tccgggacgg catcgacgat atacatctcg gtattcggag agagggctcc
      481 tatgctcgtc ggcgcgaata attcgctcga tatcaatccg ggtctgacgg ggcagaagtt
      541 attcgggctg aatggggtac ctccgatcca tgtagtcccg tatccggaga ttccagcggt
      601 ggtgaggata taatccatcc gcgttttacc ggctgtatcg acggtgaagg tgcagtaccc
      661 gtctgcatcg gtcacactgg tctgaacacc gttctccgtg tagaggtaga cggtctggcc
      721 gggtatgaag ctctctattc ccaggatagg atcgtaattg tagatgtatg ccgtgatggt
      781 ggccctatcg ctgggaaccg atgcataggc atcctggtcc caggtcgccg gccagtcgga
      841 atagctgccg atatacgtca ggttcccgct ataaagatcc tcgtgtaccg ggggagtgct
      901 ccggtgcatg agccagggct cgagcgtccc gtagacgaga accgtggtcg cctcaccaga
      961 gtactgtgcc atggcattgt tgggagagac aaactggacg attgccatgt aaccgttctc
     1021 cggcatcgtg aagttcagga gcccgatacc gttcgtcagg cggacggtgc ccgtacgggt
     1081 caccggcttc atcccggcga tctcggcaag gagcgcctgc atatctccct ggtctgtgga
     1141 gagcgtcagg gcgccagccg gcgagagcga gaggatctcg gtctcggtca gcgtggtgac
     1201 gtagacatca aagtcctggc ttacggtctt ctgcccgtgg tgcgaccagg ccatgagccc
     1261 gaggttgaca ctcatccctg gtggacagat ggctcgctcc gggagtggca tgacttcctg
     1321 gaagatatgc ggctcctcac ccacggagat gcttcctgcg cttctatcat tgatccagag
     1381 gtagaggtcg gtttcatcga gaggcctccc ggtaaggttg agtgagtatc cgatgacgcc
     1441 atctaccggg acaagcacct cttcaacgat ggtcttactg taattttcga ccgtgaaggt
     1501 gacgacctcc gtgtagggcg agccgtcggc ggcaaggagc gggtacttga tcgggacggt
     1561 cgcgctatac ggcacggagt agtcctcact ctcaaagatg ggaacgttcg ggctcgggtt
     1621 cggtgtttgc cctgaggaga tcgtgtcctt ggtcacttgg tcttccaccc gaagatcacc
     1681 ataggtgatc tggacccaca tgggcgtacc attgcggata tcggttatat ccacgggaca
     1741 gtagatgtac ccgtccgcgt cagtcctgat tgtttgatca aagacgggag tgcgctcgta
     1801 ggtataacag gcgtagtacc acacgctgac attaacctct tctcccgcca tgggttcccc
     1861 ggcggtcgtg ttccagagga ccgccatgat tgggagaaca ttgcccgtca cggtgaattg
     1921 cgtgagggtt ccgcctatga gctcacggag tatcccgtcg gacggcttca tcccgccaag
     1981 gccgccggtg acgttcaccg cctcgtaggc gttgatcctg ccggcgccgt aggcgttgtt
     2041 cggctgcatc accggcagga tggtcccatc ttcaccgatg tggaccgccg tctccatcag
     2101 ggcggccttc acgtcggcgg gcttgagggt actgttgccc gagagcatca acgccgccgt
     2161 cccggcgacg gccgggcagg acatggatgt gccctgcatg gaggcgtatt ggtcaccagg
     2221 gtatgccgat atgatgccga cgccgggggc catgacatcg ggcttgatgt agggactcgt
     2281 ccggtttacc ggccccatgc tgctgaatga ggcacggtag tccatcatgt agtcggttgc
     2341 accgacggtt atggcgtctt ttgcggtccc cggcgtgccg atggttgagg tgccaaagaa
     2401 gccatcgttt ccggcggaga ccgtgacgac ggtgccgcct ctgaccatgt tgttgatggt
     2461 ggtatcaaga acagtctctc cgcccgactg gtagcactcg gagagcccga tctcctcgac
     2521 ccggatctca gtcgctccgg tgttcgtgac gttgagctcc cagaatccgt tccagccggc
     2581 ggtgttggag gcgtagggcg ccttgaagta gtagcagttc gtctccgaat cgaagccgag
     2641 ccagtcaatc tcggcgccgt ttgcggcgtt gccctgggcg tcggtcacgg tgatcgtgag
     2701 gttctcaaag aggtctgcag tgtcgtagta ccaatcctcg accctgaccg atccgatcac
     2761 gaactgaggc tcgtaccggc aatcggaatc tccatacatc tccgatgaga cttccagttc
     2821 aacgatccgg ctctcacctt cggcaagtga gaaatacggg tcggatgtac tccgggagac
     2881 ataagcccca aggctca
//
LOCUS       DAOSIE010000193         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_30263_length_2869_cov_2.708955, whole genome shotgun sequence.
ACCESSION   DAOSIE010000193 DAOSIE010000000
VERSION     DAOSIE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30263_length_2869_cov_2.708955"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PLI69_05755"
     CDS             complement(<1..539)
                     /locus_tag="PLI69_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOG42025.1"
                     /translation="MNKKDRMKEQETPQNGHQTAREPDFEENNGLTTEEALIAEIEKL
                     KTENEELNDKYIRNVAEFDNYRRRTAKERLEMIATAGKDILVGFLPVLDDCERAIKVL
                     KESDASQAAIEGTDLIYTKLMTYLKSKGMLRIEALGQTFDTDFHEAVAQLPAHDAESK
                     NKVIDVVLQGYILNGTVVR"
     gene            complement(633..1208)
                     /gene="nadD"
                     /locus_tag="PLI69_05760"
     CDS             complement(633..1208)
                     /gene="nadD"
                     /locus_tag="PLI69_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOG42026.1"
                     /translation="MAEFIARPQKKVALFFGTFNPMHTGHLEILRYLKEHTEADEVRV
                     VVSPQNPMKQKNHSKRARLKNVKEAIARSGLDVTVSDVEYYLQEPLYTINTLRYLREK
                     EPACRHILVIGADNLTILSRWYRYRELIDEFEIWVYPREGVDAGSLSEEHNRLSDLKG
                     IRVLEGRLHNISSTEIRRAELSGKDMSAWKA"
     gene            complement(1188..1751)
                     /gene="gmk"
                     /locus_tag="PLI69_05765"
                     /pseudo
     CDS             complement(1188..1751)
                     /gene="gmk"
                     /locus_tag="PLI69_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
     gene            1867..>1946
                     /locus_tag="PLI69_05770"
     CDS             1867..>1946
                     /locus_tag="PLI69_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42027.1"
                     /translation="MISYKDLGLVNSREIFAKAMKGGYAIP"
     assembly_gap    1947..1956
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1957..2847
                     /locus_tag="PLI69_05775"
     CDS             <1957..2847
                     /locus_tag="PLI69_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOG42028.1"
                     /translation="AIPAFNFNNMEQLQAIIQACVETKSPVILQVSSGARKYANQTLL
                     RYMAQGAVEYAKELGYNIPIVLHLDHGDSFELCKSCIDFGFSSVMIDGSHLPYDENVA
                     LTKKVVEYAHKHDVTVEGELGILAGVEDDVSAEEHTYTRPEEVQDFVSKTGVDSLAIS
                     IGTSHGAFKFKPGQKPEIRLDILYEIEKRLPGFPIVLHGSSSVPQDIVAEINKYGGAL
                     KDSIGIPEEQLRAAAKSAVCKINIDSDGRLAMTAAVRKVFAEKPEEFDPRKYLGPARD
                     KLKELYKHKVINVLGSNDKA"
BASE COUNT          753 a          686 c          622 g          798 t
ORIGIN      
        1 tatcttacta ctgtaccgtt taggatataa ccctgtaaga ctacgtcaat aactttattt
       61 ttagactctg catcatgggc cgggagctgg gccacagcct cgtgaaaatc ggtatcgaag
      121 gtctggccta aagcctcaat cctcagcatc ccttttgact tgagataggt cataagtttg
      181 gtgtagatca gatcggtacc ttcgattgcc gcttgtgagg catccgactc tttaagcacc
      241 ttaatggctc tctcacagtc atccagtacg ggaagaaaac ccacaagaat atctttaccc
      301 gccgtagcaa tcatctcgag acgttccttt gcagtacgtc ttcggtaatt gtcaaattct
      361 gccacattgc ggatatattt gtcattaagt tcctcgtttt cggttttgag tttttcaatc
      421 tccgcaatga gcgcttcctc tgtcgtaagc ccgttattct cttcaaaatc tggctcccgg
      481 gctgtctgat gcccattctg aggagtttct tgctctttca tgcggtcttt cttattcata
      541 tctttctctt ttttattcat aaaactacat tatgtgccta taggaacaaa taatctgcca
      601 atgaaaaaac aggacaaaat ggcagaaaga gattaagcct tccaggcaga catatccttg
      661 cctgaaagct ccgctctacg gatttccgtg gaggagatat tatgtaacct tccttccagt
      721 acacgtatac ccttaaggtc agaaagtcgg ttgtgttctt cagagagtga gcctgcatca
      781 accccttccc tgggataaac ccaaatctca aactcgtcga tcaactcccg gtagcgatac
      841 cagcgactta ggatggtaag attgtcggca cctatgacca atatatgacg acaggcaggc
      901 tctttctcgc gtagatagcg tagagtattt atggtgtaga gcggctcctg aaggtagtac
      961 tccacatctg aaactgtcac atccagaccg ctgcgcgcta tggcttcctt cacattctta
     1021 agtctggctc tttttgagtg gttcttctgc ttcatcggat tctgcggcga aaccaccacc
     1081 cttacctcgt ctgcctccgt atgctcctta agatatctca aaatctcgag gtggccggta
     1141 tgcatcgggt taaaggtacc gaagaagagc gcaacctttt tctgtgggcg tgcaatgaat
     1201 tcagccacac attcctccgc ttccctcaaa caggttgtca ggtcatcgtt aatcaacacc
     1261 acatcaaatc tggatgcata gcgcacctct ttccttgcct ttaacactct ctcctctata
     1321 gcctcaggag tgtcggtacc tctcagctgc aatcttttgc ggagctccct tatggagggg
     1381 gccttgataa aaaccgaaag ggcctggtcg cccagaagct tcttgaggtt gattcccccc
     1441 ttgacatcta cgtcgaagag aataatgttg ccgttagacc agatacgttc catttccgac
     1501 ttaagcgtgc cataaaagga gcctgcatag acctcctcat actccacgaa ttcgcccgct
     1561 gaaatccttc tacgaaactc ttcctcagat aaaaagtagt actccctacc gttcttctcc
     1621 ttccctctgg gggctctgga ggtggcggaa atggagaatt cgaggttatc aaatttagtc
     1681 aggagatgat tgaccacagt acttttgccc gacccggagg gtgctgagaa gattatgact
     1741 tttttgttca tgcctatagt gcctcgagtt gcttggaata tgcaaaaata gtgatattcc
     1801 ccaaaaaact attatctttg tgaggtttat ttataaaaca actaaaccga taatagttta
     1861 aataatatga tttcttataa agatttgggc cttgtaaatt ccagagagat atttgcaaag
     1921 gcaatgaaag gtggttatgc catcccnnnn nnnnnngcca tccccgcatt caattttaac
     1981 aatatggagc aattacaggc catcattcag gcctgtgtgg agaccaagtc tccggttatc
     2041 ctccaggttt cgagtggtgc acgcaagtat gcaaatcaga cacttctcag atatatggca
     2101 cagggtgccg tagaatatgc aaaggagctg ggatataata ttcccatcgt acttcatctc
     2161 gaccatggtg actcttttga actctgtaag tcatgtattg acttcgggtt ttcatctgtt
     2221 atgatagacg gatcacatct tccttatgat gagaatgtag ctctgaccaa gaaagttgtg
     2281 gaatatgctc acaaacatga tgtaacggta gagggtgaac tgggaattct tgccggagta
     2341 gaagatgacg tatctgctga ggaacatacc tacaccaggc cggaagaggt tcaggatttc
     2401 gtaagcaaaa cgggagttga ctctcttgca atttccatcg gcacatcaca cggagcattc
     2461 aaattcaaac ctggtcaaaa acctgaaata cgcctcgata tcctttacga gattgaaaag
     2521 cgcctccccg gtttccccat tgtacttcat ggctcatcca gcgttcctca ggatattgtc
     2581 gctgagatca acaagtatgg cggagctcta aaagactcca tcggtattcc ggaagagcag
     2641 cttcgggcag ccgcaaagtc tgctgtctgc aagatcaata ttgatagcga cggccgtctg
     2701 gccatgactg ctgcagtgag aaaggtattt gctgagaaac ctgaggagtt tgatccccgt
     2761 aaatatctgg gaccggcacg tgacaaacta aaagagcttt acaagcacaa ggtgataaat
     2821 gtgctgggta gcaacgataa agcataagaa aaaacgggga tcttccccg
//
LOCUS       DAOSIE010000194         2867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_30289_length_2867_cov_4.328236, whole genome shotgun sequence.
ACCESSION   DAOSIE010000194 DAOSIE010000000
VERSION     DAOSIE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30289_length_2867_cov_4.328236"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLI69_05780"
     CDS             <1..682
                     /locus_tag="PLI69_05780"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOG42029.1"
                     /translation="NSLFTDQGRFETALSEVRTADDYRADPLVAAINSTKMITYNQYK
                     DKVDRGIKLSRLENNYKNLLYQMYLEKGVPQYPDANSTMRLSYGTVGPLCRCDGVICL
                     ERSTVRGILEKYDPDRYIYSLKPNLKALYEARDWGRWADPYTGELYINFVTDNDITGG
                     NSGSPVLNGKGELIGLAFDGNKESLAGDTYYDPVMNKCVCVDIRYVLWVLDKYAGMEH
                     IVAEMLGE"
     gene            complement(685..1455)
                     /locus_tag="PLI69_05785"
     CDS             complement(685..1455)
                     /locus_tag="PLI69_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOG42030.1"
                     /translation="MYKICIDMGCWNEIVSGTRYSFKALGDLFFPRRCAVCETLLACE
                     EVNICDDCRGDIPYTWFWSWPENPAEERMWSKVGIESAASLFFYRYTGGYGHILHKFK
                     YGCNIPLGMAMGRDLGEKMVQGGRFKDIDAIVPIPLHWRRQWHRGFNQAEIVSRGVAV
                     GLGALPNGRIIPVIRNLVKRVKNTSTQTRLTGKEKAENVKNAFRVEKKVALQLLSKGI
                     NHILLVDDVLTSGSTLAACATPLMQYFRVSVATIGFVE"
     gene            1485..1925
                     /locus_tag="PLI69_05790"
     CDS             1485..1925
                     /locus_tag="PLI69_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42031.1"
                     /translation="MLDERIIEFISEHHVMSLACAANGDLWCASCFYVFLEEEMSFVI
                     TSEEHTRHAQLFMENPVVAGTIALETETVGKIRGLQFRAIVKRCDGSLFDKYRIAYLK
                     RFPYAILKGGEIWRLVLTELKYTDNRLGFGKKLHWKSDKKQYLC"
     gene            1928..2713
                     /gene="cdaA"
                     /locus_tag="PLI69_05795"
     CDS             1928..2713
                     /gene="cdaA"
                     /locus_tag="PLI69_05795"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOG42032.1"
                     /translation="MLDFISISLVDIFDIILVGLLIFQIVRLIRGTAAMSIFAGILLL
                     YVIWLVVSAFNMKLLSSLMGQVLGVGVIALIVIFQPEIRRFLLHLGSRYASANRGSFF
                     RRLFKGRSGVNVVSPELLEELTEACRFMSQEHTGALIVLKHRTSLAAIIETGDEVDAK
                     VSRRLLLNIFFKNSPLHDGALIIANERIQAVRCTLPISANPNIPAQYGLRHRAAVGIT
                     EQSDASAIVVSEETGKISFVSNGILKPINSITELRTAIENSYR"
BASE COUNT          778 a          624 c          681 g          784 t
ORIGIN      
        1 aaattcgcta tttacggatc aaggcagatt tgaaactgct ttgtctgaag tgcgtactgc
       61 tgacgattac cgtgcagatc ctctcgtagc tgctatcaat tccacaaaaa tgatcaccta
      121 caatcaatac aaggataaag tggatagagg aataaaactc agcaggctcg aaaacaatta
      181 caaaaatctg ctctatcaga tgtaccttga aaaaggtgtt cctcaatatc cggatgccaa
      241 ctccacaatg aggctctctt acgggacagt cggtccgctc tgtcgctgtg acggtgtgat
      301 ttgtcttgaa agaagtacgg tacgtggcat cctggaaaaa tatgaccccg acagatatat
      361 ttattcactc aaacccaatc tgaaagccct ctatgaggcc cgggattggg gacgatgggc
      421 cgatccatac acgggagaac tctatatcaa ttttgtaacc gacaatgata taacgggagg
      481 caattccggc agtcccgtgc tgaatggcaa aggagaactg atagggctgg catttgatgg
      541 aaataaggag tctcttgccg gtgataccta ttatgaccct gtgatgaata aatgcgtctg
      601 tgtcgatata cgttatgtgc tctgggtttt ggataaatat gcgggaatgg agcatatagt
      661 ggcggaaatg ctgggagagt agagtcactc tacaaatcct atggtggcca cgctaaccct
      721 gaaatattgc atcagaggtg tagcgcaggc tgccagggtg gaaccggaag tcagtacgtc
      781 atccacgagc aatatgtgat ttatgccttt cgaaaggagt tgtaaggcca ctttcttctc
      841 gacccgaaag gcatttttta cattttcagc tttttctttt ccggtgagcc gagtctgggt
      901 agaagtgttc tttacacgct ttacaaggtt tcttataaca ggtatgattc ttccattggg
      961 tagtgctccc aaccctacgg ctacgccccg cgaaacaatc tctgcctgat tgaagccgcg
     1021 atgccattgc ctgcgccagt gcaggggaat gggaactatg gcatcaatat ccttgaaacg
     1081 acctccctgt accatttttt caccgagatc tctgcccatt gccattccaa gggggatatt
     1141 gcagccatat ttaaatttgt ggagaatatg gccgtatcca ccggtgtagc ggtagaaaaa
     1201 aagtgacgct gcactctcaa tacctacttt tgaccacatc cgttcttcag ccggattttc
     1261 cggccagctc cagaaccagg tataaggaat gtctccgcga caatcgtcgc aaatattaac
     1321 ttcttcacaa gcaagaagag tttcgcaaac tgcgcatctg cgggggaaga agaggtcccc
     1381 aagcgcttta aaagagtatc tcgtcccact gacaatctca ttccaacagc ccatatcgat
     1441 acatattttg tacaaaccta caaaaaaagc ttaattttgc agccatgctt gatgaaagaa
     1501 tcattgaatt tatctcagag catcatgtga tgtcacttgc ttgtgccgca aatggggatt
     1561 tgtggtgtgc gagttgtttt tatgtttttt tggaagaaga gatgagtttt gtcattacat
     1621 ctgaggagca tacgcgacat gcacaacttt tcatggaaaa ccccgtggtc gcaggcacta
     1681 tagcgctcga aacggaaact gtaggcaaga tccgtggttt gcagttcaga gctattgtta
     1741 agagatgtga cgggtcgctt tttgataaat atagaatagc ctatctcaaa cgcttcccct
     1801 atgcaatcct taaaggggga gagatatggc gcctggtact taccgagctt aaatataccg
     1861 acaaccgtct gggcttcggc aaaaaacttc actggaaatc cgacaaaaaa cagtaccttt
     1921 gttgatcatg cttgatttta tttccatatc cttagtggac attttcgaca ttatacttgt
     1981 cggcctgctc attttccaga ttgtcagatt gatacgtggc acagcagcta tgagtatttt
     2041 tgcggggata cttcttcttt atgtgatatg gctggtggtt tccgccttca atatgaagct
     2101 tctctcttca cttatggggc aggtacttgg agtgggtgtg attgcattga ttgtcatttt
     2161 ccagccggaa attcgccgtt ttttacttca tctgggctcc agatatgcat ctgcaaacag
     2221 aggtagtttt ttccgtcgcc tttttaaggg ccgtagtggt gtcaatgtag taagtcccga
     2281 actcctggaa gaattgacgg aggcttgcag gtttatgtca caggagcata ccggtgcgct
     2341 tatagtgctt aagcacagaa cttcgctggc tgccattata gagacgggtg atgaggttga
     2401 tgcaaaagtg agccgcagat tgcttctcaa tatatttttt aagaactccc ccctgcacga
     2461 tggagctctc attattgcca atgaaaggat tcaggcagtc agatgtactc ttccaatctc
     2521 ggcaaacccc aatatacccg cccaatatgg tttgcgccat agagctgccg tcggaatcac
     2581 cgagcagagc gatgcaagtg ccatagtggt ctctgaagag acaggtaaga tttcgtttgt
     2641 ttcaaacgga atactcaaac ctataaacag tatcactgag cttagaacag ccattgaaaa
     2701 ctcatacaga taagggatga agggatgata ctggagcaga aaataggttt tgacaggatt
     2761 aggagaggga tcgccgagcg ttgctccacg cagtatgcca ttgacaaagt atgggatgaa
     2821 gagatttccc gcgacgccag ggagatagat ttgcggcttc aactaac
//
LOCUS       DAOSIE010000195         2848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_30556_length_2848_cov_4.201575, whole genome shotgun sequence.
ACCESSION   DAOSIE010000195 DAOSIE010000000
VERSION     DAOSIE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30556_length_2848_cov_4.201575"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /gene="rseP"
                     /locus_tag="PLI69_05800"
     CDS             <1..1323
                     /gene="rseP"
                     /locus_tag="PLI69_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOG42033.1"
                     /translation="NKFYLFFNPSFSIMRAKKIGGKWRFKFFSKNLSNFEGDKKIDIN
                     TLPEDDWRRYPENTEYGIGWLPLGGYCSIAGMIDESMDKEAMKLPPQPYEFRTKPAWQ
                     RFFIMFGGVLFNFILAVLLYGAIMNVWGDEYLRNEDAIYGIAVNDLSYEIGFRDGDRI
                     LDFDGVPTENFSMLQVDMVRSQAEQARVLRGNDTLTINIDPQYISAILNTPGMFDLAF
                     PFVVGGFSEGSINADSGLEIGDEIKGIDSVSMFLVQEVRKELLRHKESSVVASVSRSG
                     DLMDIPLQVDSAGMIQVMLDSDLTKFFTITSKEYNFFTAIPAGCKKAWNNITNYVQEL
                     GLIFSPKTKAYKSVGSFIAIGKIFPGDWDWYRVWTLTAMLSIMLGVLNLLPIPALDGG
                     HILFLLIEIITRRKPSDKVLEVAQTIGMILLIALMILAFGNDIRGLFT"
     gene            1336..2346
                     /locus_tag="PLI69_05805"
     CDS             1336..2346
                     /locus_tag="PLI69_05805"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOG42034.1"
                     /translation="MKRKNLTTLTLYVMTAFALTALHSCKGEPGKILPPASGKAGEIA
                     VISPRVHWEAEPGTTIRSILAAEFAYLPQREPTYTLFNVPPKSFNKVFQVHRNLLNIV
                     ISDTVSSNRMGISRDIWAKSQIMVSVYAKDAESAAKIIGENAEKLLSNYETAERNRVI
                     NNARNHANPTLKKFVTAVVGGSPDFPRDFTEKMHTNNFFWISYETSYTNQGIFVYTFP
                     YQGEWQLTTEYLVAKRNEFLKENVPATAENSYMITHPTVTPGFAIKEYNGLNFIEMRG
                     LWDTQNDFMGGPFVSHTYVSPDNKNVVVLEGFVYAPKYEKRNYLRQVEYIISSFEWSK
                     GE"
     gene            2421..2495
                     /locus_tag="PLI69_05810"
     tRNA            2421..2495
                     /locus_tag="PLI69_05810"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2454..2456,aa:Glu,seq:ttc)
     gene            2518..2772
                     /gene="rpsT"
                     /locus_tag="PLI69_05815"
     CDS             2518..2772
                     /gene="rpsT"
                     /locus_tag="PLI69_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOG42035.1"
                     /translation="MANHASAEKRYRQSEKRRLHNRYYARTTRNAIKAIRNTTDKAAA
                     EASLPKVFAMIDKLAKTNVIHKNKAANLKSGISIHVNKLL"
BASE COUNT          840 a          656 c          620 g          732 t
ORIGIN      
        1 aacaaatttt acctcttttt taacccctct ttttccatta tgcgcgccaa gaaaatcggc
       61 ggcaaatggc ggttcaagtt tttctcgaaa aatctttcca attttgaggg agacaaaaaa
      121 atagatatca acaccctccc cgaagatgac tggagacgtt atcccgagaa tacggaatat
      181 ggcatagggt ggctccctct cggaggttat tgctcaatag cgggcatgat tgacgagtcg
      241 atggacaaag aggcgatgaa actccctccg cagccctacg aattccgaac taaacctgcc
      301 tggcagagat ttttcataat gtttggaggt gtgctgttca atttcatcct tgccgtattg
      361 ctctacggag ccattatgaa tgtatgggga gacgaatacc tgcgcaatga ggatgctatt
      421 tacggaattg cggtcaacga tctttcttat gagataggat ttcgggacgg tgacaggatc
      481 ctcgactttg acggagtacc taccgaaaac ttttccatgc tccaggtcga tatggtccgt
      541 tctcaggctg aacaggcgag ggtccttcgc ggtaacgaca cactgaccat caatattgat
      601 ccccaatata tttcagcgat actcaacact ccgggaatgt ttgacctcgc cttccctttt
      661 gtggttggcg gatttagcga aggctcaata aatgctgatt ccggacttga aataggtgac
      721 gaaatcaaag gcattgactc cgtgtcgatg tttctggtgc aagaggttcg gaaagagttg
      781 cttcgtcata aagagagttc ggttgtcgct tcagtcagca ggagcggtga tctgatggat
      841 ataccccttc aggtagattc ggcaggtatg atccaggtga tgttggatag cgacctgaca
      901 aaattcttta ctataacaag taaagaatac aactttttta ccgccattcc tgccggttgt
      961 aaaaaggcct ggaacaacat taccaattat gttcaggaac tggggctgat attttcaccc
     1021 aaaaccaagg cctacaaatc tgtgggcagt ttcatagcca ttggtaagat attccccggc
     1081 gactgggatt ggtacagggt gtggacgctg acagcgatgc tctcaattat gctcggagta
     1141 ctcaatctgc tgcccatacc ggcattggac ggaggccata ttctattcct tctcattgag
     1201 atcattactc gccgcaaacc gagtgacaag gtgttggagg tggctcagac aataggtatg
     1261 atcctgctga ttgccctaat gatactcgcc ttcggcaatg acatacgagg attattcaca
     1321 taacataata taacaatgaa aagaaaaaac ctgacaactc tgacgctcta cgtgatgacg
     1381 gcatttgccc taacggcact tcactcatgt aaaggagagc ccggaaagat actccctccg
     1441 gcgagcggaa aggccgggga aattgccgtc atatccccca gagtccattg ggaagccgaa
     1501 ccgggtacta ccatcagaag catactggct gccgagtttg cctatcttcc ccagagggaa
     1561 cctacctaca ccctcttcaa tgtgcctccc aagtcattca acaaggtatt ccaggtacac
     1621 cgtaacctcc tcaacattgt aatttcggac accgtttcct ccaatcgcat gggtataagt
     1681 cgtgatatct gggccaaatc acagattatg gtctcggtct atgccaagga tgcagaaagt
     1741 gctgcaaaaa taattggtga aaacgctgag aagcttctct ccaattatga gaccgcagag
     1801 cgcaaccgcg ttatcaataa tgcgagaaat cacgcaaacc ccactctgaa gaaatttgtt
     1861 accgccgtag ttggaggctc tcccgatttc ccgagagatt tcacagaaaa gatgcatacc
     1921 aataattttt tctggatctc ctacgagacc tcctatacca atcagggcat atttgtctat
     1981 acattcccct atcagggcga atggcagctc actactgaat atctcgtggc caaaagaaat
     2041 gaatttctca aggaaaatgt ccctgctact gcagaaaaca gctatatgat tacccatcct
     2101 acagtcacac cgggctttgc cataaaagag tacaacggtc ttaactttat cgagatgaga
     2161 ggtctctggg atactcagaa cgactttatg ggaggtccat tcgtttccca cacttatgtc
     2221 tctccagaca acaaaaacgt tgtggtgttg gaaggattcg tatatgctcc aaaatatgaa
     2281 aagcgcaact atttgcgtca ggttgagtat attatctcct catttgagtg gagcaaagga
     2341 gagtaattat tagataaaat ttgcaatagc aaaaattctt tatatctttg cactcccaaa
     2401 aaacaaaaat cgggagccat ggtgggttcg tctaacggct aggactcaag attttcattc
     2461 ttgcaatagg ggttcgattc ccctacccac taccacaaaa aataaaaaag taaaataatg
     2521 gcaaaccacg catcagcaga aaagcgttac cgtcaaagcg agaaacgcag attgcataac
     2581 aggtattatg caagaaccac aagaaacgcc atcaaggcaa tccgtaacac aaccgacaaa
     2641 gcagcagcag aggcttcatt gcccaaggtg tttgcgatga ttgacaaact ggccaagacc
     2701 aatgttattc acaagaacaa agcagccaat ctcaagagcg gcatctccat tcacgtgaat
     2761 aagcttttat aaattgtctt tttgacatat taaatagaga aaactttcca atctttggaa
     2821 agtttttttt atatcgttaa ggaacaat
//
LOCUS       DAOSIE010000196         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_31176_length_2806_cov_3.013450, whole genome shotgun sequence.
ACCESSION   DAOSIE010000196 DAOSIE010000000
VERSION     DAOSIE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31176_length_2806_cov_3.013450"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..>2806
                     /gene="purL"
                     /locus_tag="PLI69_05820"
                     /gene_synonym="purI"
     CDS             14..>2806
                     /gene="purL"
                     /locus_tag="PLI69_05820"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOG42036.1"
                     /translation="MNIEGIVRMEEYFPVSGPDAEIDRMLQRLYNGLDQEIFTTDNIF
                     EPVKQITDLDKYNIEEGLALSEEEIGYLHALSDRIGRPLTDSEVFGFSQVNSEHCRHK
                     IFNGTFIIDGKEMPSSLFSMIKKTSKLNPDRIVSAYKDNCAFVEGPLVHLFHPASGDR
                     PDFFVKSKHETVISLKAETHNFPTTVEPFNGAATGTGGEIRDRIAGGKGAFPIAGTAV
                     YMTSYPRFEGEKERVWECRPPRKWLYQTPLEILLKASNGASDFGNKFGQPLICGSLYT
                     FEHSEEGIDYGFDKVIMLAGGVGYAKKKDSLKGSPEADDEIILLGGDNYRIGMGGGAV
                     SSVATGEFANAIELNAIQRSNPEMQKCVYNTIRAIAEKEYNTIISIHDHGAGGHLNCL
                     SELVEHSGGTIDISCLPVGDPTLSAKEIIGNESQERMGLVMKSKDVDELSRIAERERA
                     PLYRIGKTTGQHNFTLKNFQTGEVPIDLTIEDMLGTTPGTVMTDNTVNYNYKGVPSPS
                     NFSADELISSIEKVLLIESVACKDWLINKVDRSVTGLIACQQSTGEIQLPLNNLGVTA
                     IGYDDNEGIATSIGHVPAVALIDSEAGSRMAIAEALTNMVWTPIEGGIKSVSLSANWM
                     WPCKNEGEDVRLYNAVRAASDFAIALGINIPTGKDSLSMTQKYPDGTKVLSPGTLIVS
                     TVSPAKDIRKTVHPNLISKRSDILWIPFTHTDAYPLGGSIYAQTKCYLGNDTPDVDDA
                     AYFAKCFNTIQELISKGHILSGHDISAGGLVTTLLETCFPNTTGGLYIDLSFTSPDAL
                     FCEAPGVIIQTSGGEAASLLTRQGINFYEIGEWQQERKLTLKLSSYTLEIDIDKMRDT
                     WYKTSYLFDKLQSGEEKAAKRFVQYKLQPLTYRFPNGFSGKRPEQPSKRPVAAIIREK
                     GSNGEREMAWALYLAGF"
BASE COUNT          822 a          692 c          655 g          637 t
ORIGIN      
        1 gattgcacat aatatgaata tcgaaggtat tgtgaggatg gaagagtatt ttccggtatc
       61 cggaccggat gctgagattg acaggatgct ccagcgcctc tacaacggac ttgaccagga
      121 gatttttaca accgataata tatttgaacc ggtgaaacag atcaccgatt tagataaata
      181 caacatagag gaaggtctgg ctctttcaga agaagaaatt ggctatttgc acgctctgag
      241 cgaccgtatt ggcagacctc tcacagattc tgaagtgttc ggcttttcac aggtcaattc
      301 cgaacattgt cgccacaaga ttttcaacgg tacattcatt atagacggaa aggagatgcc
      361 ttcatccctt ttcagtatga ttaagaagac atcaaaactc aatccggata ggatcgtctc
      421 tgcgtacaag gacaattgtg catttgtcga gggtccgctg gtccaccttt tccaccctgc
      481 atcaggcgac agacccgatt tttttgtaaa aagcaaacat gagaccgtta tttcgctgaa
      541 agcggaaaca cacaattttc ccactacggt ggagcccttc aacggagcag ctacaggtac
      601 cggaggagag atcagagacc gcattgcagg tggcaaagga gccttcccaa tagcgggaac
      661 tgcggtttat atgacctcgt atccccgctt tgaaggtgaa aaagagagag tatgggagtg
      721 cagacctccc agaaaatggc tctaccaaac tccattggag atactcctca aggcctcaaa
      781 cggcgcatca gactttggca ataagtttgg acaacccctt atctgcggct cgctctacac
      841 cttcgaacac tcggaagagg gaattgatta tggctttgac aaggtgataa tgctagcggg
      901 aggtgttggc tacgccaaaa agaaagactc cttaaagggt tctcccgagg cagatgatga
      961 gatcattctg ctcggaggcg acaattatcg cataggaatg ggtggaggag ccgtatcgtc
     1021 ggtagccacc ggagaatttg ccaacgctat agaacttaat gcaattcaac gctccaaccc
     1081 ggagatgcag aagtgcgtct acaataccat tagggctatc gcagagaaag agtacaacac
     1141 cataatatca atacatgacc acggagccgg cggacatctc aactgcctct cggagcttgt
     1201 agagcatagt ggcggcacca ttgacatcag ctgcctcccc gttggcgatc ctacactttc
     1261 cgctaaagag attatcggta acgaatctca ggagcgtatg ggactggtga tgaagagtaa
     1321 ggatgtggat gaacttagca gaatagccga aagagaacgc gcacctttat atagaatagg
     1381 caaaactacg gggcaacaca acttcacttt aaaaaacttt cagacgggcg aggtacctat
     1441 agacctcact atagaagata tgcttggaac tacacccgga acagtcatga cggacaacac
     1501 cgtcaactac aattacaaag gtgtaccctc tccaagcaat ttttctgcag atgagcttat
     1561 atcctctata gaaaaagtac tgctaataga gtcagtggcc tgcaaggatt ggcttatcaa
     1621 caaggttgac cgctccgtta ccggactgat tgcctgccaa cagagtacgg gggaaatcca
     1681 acttccgcta aacaatctcg gagttacagc gataggttat gacgacaacg agggcattgc
     1741 cacctctatc ggtcatgttc ccgccgtagc cttgatagac tctgaagccg gttcaagaat
     1801 ggcaatagcc gaggctctca ccaatatggt ttggactccg atagagggcg gtatcaaaag
     1861 tgtctctttg agcgccaact ggatgtggcc ctgtaaaaac gaaggcgagg atgtacgcct
     1921 atacaacgcc gtacgcgcag catctgactt tgcaatagca ctcggcatca acattcccac
     1981 cggaaaagac tccttgtcga tgacacaaaa gtatcccgat ggtacaaagg tactctcccc
     2041 cggaactctg atcgtctcca ctgtgagccc tgcaaaagat atccgaaaga cagtccaccc
     2101 caacctgatt tccaagcgca gcgacatcct ctggatccca tttacacata ccgatgcata
     2161 tccactcgga ggctcaattt atgcacaaac aaagtgttac ctgggtaatg atactcctga
     2221 cgtagatgat gccgcatact ttgcaaaatg tttcaatacc atccaggagc tgataagcaa
     2281 aggccatatc ctctccggac atgacatttc agccggaggc ctggttacta ccctgttgga
     2341 aacctgtttc cccaatacaa ccggaggact ctatattgac ttgagtttca cctcaccgga
     2401 tgcccttttc tgcgaggcac cgggagtcat catacagacc tcaggaggag aagctgcctc
     2461 cctgctgacc cgccagggca tcaatttcta cgagatcgga gaatggcaac aggaaaggaa
     2521 gctgactctc aaactctcat catatactct ggagatcgat atcgacaaga tgcgcgatac
     2581 ttggtacaaa acttcatatc tattcgataa actacagtca ggagaagaga aagctgccaa
     2641 gagattcgtt caatacaaac tccagcccct cacctatcga tttcccaatg gattctcagg
     2701 aaagagacct gaacagccct caaagcgtcc tgttgcagca attatcagag aaaaaggatc
     2761 caatggagaa agggagatgg catgggctct ctacctggcc ggcttc
//
LOCUS       DAOSIE010000197         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_31232_length_2802_cov_4.702585, whole genome shotgun sequence.
ACCESSION   DAOSIE010000197 DAOSIE010000000
VERSION     DAOSIE010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31232_length_2802_cov_4.702585"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PLI69_05825"
     CDS             <1..1085
                     /locus_tag="PLI69_05825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOG42037.1"
                     /translation="KIDSIFSELFPSDEPGAAVLVLQGDEIIFDKGYGIADLESGAAI
                     DGNTFFNIASVSKQFTAVAVLQLAEQGKLSMEDNLKSYFPEYKADFYEKIQIKHLLSH
                     SSGIPDARDRSDKERNLIATEEIVMQYLPDLDFLHFEPGTEYEYINPTFLILASIVEK
                     VSGMPFADYVKEHIFIPAGMEKTLYFEAEKEALIPNMAHGYEYEDVSNMPEERTASEI
                     DKDAKKEWYEYDYGEVNFFATKADGGMYTSTHEFVLWEKALRNHTVLGEEMLNAAHTP
                     QIKVSGSKFSSYQNRPNTWYGYGWFIEPATESSPLKIYHTGDNGGFKILAARYPESET
                     LVLVFANRADLNRYAVMQQIEEALGL"
     gene            complement(1214..1687)
                     /locus_tag="PLI69_05830"
     CDS             complement(1214..1687)
                     /locus_tag="PLI69_05830"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOG42038.1"
                     /translation="MRKIQEKFMRLAIDLAAENIKNGGGPFGAVIVKDGEVVATGCNR
                     VTASNDPTAHAEVSAIRAACKALDTFVLEGCEIYSSCEPCPMCLGAIYWAHLDKLYYA
                     ANKHDAADAGFDDSFIYEELELKQEDRRLKAEVLLQNEALRVFESWKAQEDKVEY"
     gene            complement(1687..2343)
                     /gene="fsa"
                     /locus_tag="PLI69_05835"
     CDS             complement(1687..2343)
                     /gene="fsa"
                     /locus_tag="PLI69_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOG42039.1"
                     /translation="MKFFIDTANLEQIKEAQELGVLDGVTTNPSLMAKEGIKGDAAIK
                     AHYQQICEIVDGDVSAEVFGTEYDDMIRQGEELAALHPQITIKLPCTKDGIRAVKYFA
                     EKGVKTNCTLVFTVGQALLAAKAGATYVSPFVGRLDDISSDGVALIKEIVDVFNYYGY
                     QTQVLAASIRHTQHIIDCMKAGADVATCPLKAILGLLNHPLTDKGVEIFVADARKMEG
                     "
     gene            complement(2375..>2802)
                     /locus_tag="PLI69_05840"
     CDS             complement(2375..>2802)
                     /locus_tag="PLI69_05840"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOG42040.1"
                     /translation="IQINFYPAFLDNEYAASDIWDLEDIFEATDKAWQKDKSNVELQK
                     QLEKSVIAMNAVKKPSYKRVVDHIDHVVSLVGVKHVGLGSDFDGINTTPKGLEDISKM
                     QRIIIELRRRGYKEHEIKAIAGGNFFRVMDAAQRIADEE"
BASE COUNT          738 a          643 c          648 g          773 t
ORIGIN      
        1 cgaaaatcga ttccatcttt tctgaactat ttccgtcaga tgagcccggt gcagcagtac
       61 ttgtcctaca gggagatgag attatttttg acaaaggtta cggcatagct gacctcgaga
      121 gtggagcggc cattgacggc aatacatttt tcaatattgc ctcagtttcg aagcagttta
      181 cggcagtggc ggtactacaa ctcgccgagc agggcaaact ttcaatggag gataatttaa
      241 agagctattt ccctgagtat aaggctgatt tttatgaaaa aattcagatc aaacacctcc
      301 tctcccactc ctcaggcatt cccgatgcac gcgatagaag cgacaaagag cgcaatctga
      361 tcgctacgga agagattgtg atgcagtatc ttcctgacct cgatttcctt cattttgagc
      421 cggggacaga atatgaatat atcaatccta catttctgat tctagcctcc atagttgaaa
      481 aagtttcagg gatgccattt gccgattatg ttaaagagca tattttcatt ccggcaggaa
      541 tggagaaaac tctctatttt gaggctgaaa aggaggctct aatccccaat atggctcacg
      601 gatatgaata tgaggatgtc tcaaatatgc ccgaggagcg tactgcaagc gaaattgata
      661 aagatgctaa gaaagagtgg tacgaatatg attatggtga ggtcaacttt ttcgcaacca
      721 aggcagacgg cggcatgtac acttcaaccc atgaattcgt attgtgggaa aaagcgctac
      781 gcaaccatac ggttcttgga gaagaaatgc tgaatgctgc acatacgcca cagataaagg
      841 taagcggcag caaattcagc agttatcaaa accgtcccaa tacctggtat ggatatggtt
      901 ggtttattga gcctgcaacc gagtcatcac ctctgaaaat ttaccacacc ggcgataatg
      961 gcggatttaa aatccttgct gcacgttatc ctgagagtga aactctcgtt ctggtattcg
     1021 ccaaccgtgc agacttgaac cgttatgccg taatgcaaca gatagaggaa gcgttagggt
     1081 tgtagagttt agagtttaga gtttggagtt tggagtttgg agtttggagt ttggagtttg
     1141 gagtttggag ttttaagttc caagtagtcc gccactacat actacatact acatacttca
     1201 ggatacagac aactcaatac tccaccttgt cttcttgagc cttccaggat tcgaaaaccc
     1261 ggagggcttc attttgtagc agaacctctg cttttaagcg gcggtcttcc tgctttagtt
     1321 cgagttcctc atatatgaag gagtcgtcga agccggcatc ggcggcatcg tgcttgtttg
     1381 cggcataata aagcttatcg agatgagccc agtagatggc cccgaggcac ataggacagg
     1441 gttcacaaga ggagtagatt tcgcatcctt cgagcacgaa cgtatcaagc gctttgcagg
     1501 ctgcacgaat tgcactcact tccgcatgag ctgtagggtc atttgaagct gtcacgcggt
     1561 tgcatcccgt ggcgaccact tctccatcct tgacgattac ggcaccgaag ggacctcccc
     1621 catttttaat gttttcagcg gcgaggtcaa tcgcaaggcg catgaatttt tcctgaattt
     1681 ttctcatcag ccttccattt tcctggcatc cgccacgaag atttccacac ctttatctgt
     1741 caagggatgg ttgagaagac ccaggatggc ctttagagga caagtggcga cgtcggcacc
     1801 ggctttcata caatcaatga tatgttgagt gtggcgaatt gaagctgcga gaacctgcgt
     1861 ctgataaccg tagtagttga aaacatctac gatttctttg ataagagcaa ctccatcgct
     1921 cgaaatgtcg tccagacgcc ctacaaatgg agagacatag gtggctcccg ctttggcagc
     1981 gagcagcgcc tgtcctacag taaaaacaag ggtgcaattg gtctttacgc ctttttcggc
     2041 gaaatacttt actgctcgga tgccatcttt agtacaggga agcttgatgg taatctgagg
     2101 atgcagcgct gcaagctcct caccttgacg tatcatatcg tcgtactccg taccgaatac
     2161 ttccgcgctt acgtcaccgt caacaatttc acatatctgt tgatagtgag ccttgatggc
     2221 ggcatcgccc ttgatgccct cctttgccat aagtgaaggg ttggttgtaa ctccatccag
     2281 gactcccagt tcctgagcct ctttaatctg ctcgagattg gcagtatcta taaaaaattt
     2341 catatctgat aaaataattt gttgcaaaaa aagattattc ttcgtcagca atcctctgag
     2401 cggcatccat gacacggaag aaattgccac cggcaatcgc cttgatttca tgctccttat
     2461 agccgcgcct gcgaagttcg atgattattc gctgcatttt gctgatatcc tcgagacctt
     2521 ttggagtggt gtttataccg tcgaaatcac tgccgagtcc aacatgttta acgcctacca
     2581 gactgaccac atggtctata tggtctacga cccttttgta ggagggcttc tttacagcgt
     2641 tcattgcgat gacggacttt tcgagttgtt tctgaagctc gacattggat ttatctttct
     2701 gccaggcctt gtcagtagcc tcaaaaatat cctcaaggtc ccagatatca gatgctgcat
     2761 actcattatc cagaaaagcg ggataaaaat tgatctgaat ta
//
LOCUS       DAOSIE010000198         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_31282_length_2798_cov_3.481954, whole genome shotgun sequence.
ACCESSION   DAOSIE010000198 DAOSIE010000000
VERSION     DAOSIE010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31282_length_2798_cov_3.481954"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLI69_05845"
     CDS             <1..558
                     /locus_tag="PLI69_05845"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOG42041.1"
                     /translation="RRDMTYEKTGLPWILPSPQVPQAQNAIGYPCAGIVGDFSGHYLN
                     IGVGYTLPFLTFAAEWVDADDLKAELDSYNIPGVAWRTIHYKPFFGSSQGKLLHGVQY
                     FFTDYEIAPLTLINFYVMQAIHKLYPDHNPYEGGKNRTTDIVCGTDSIRLKFGENFLV
                     SDMIDYWNKDVEHFRELSKKYYLYE"
     gene            767..1675
                     /locus_tag="PLI69_05850"
     CDS             767..1675
                     /locus_tag="PLI69_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG42042.1"
                     /translation="MKKVVALYFSPTGGTAKVVRSMARAFAAAMGCDYSEINVTLPEQ
                     RKSAPAFGADDVVFFGVPTYIGRVPNLIRPFFASIKGCRAIAETVEEAVAGVVDVSSD
                     VVTGGALGIPVVTYGNRAYDDALIELRDIMEENGFRTIAAAAFPSEHSFSTTLAEGRP
                     NEKDLQLAKEFAGKVAETITKNPQLISDSHTPIAVPGNPAPYSYYKAVISDNKSIDIR
                     KVKPITDPEKCTKCGICALLCPMGSINISDPSITDGICIKCGACIKRCPQGAKSITDP
                     DYLEHKRLLEEEYGNVIKKAELFYTI"
     gene            complement(1668..>2798)
                     /locus_tag="PLI69_05855"
     CDS             complement(1668..>2798)
                     /locus_tag="PLI69_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOG42043.1"
                     /translation="FTAEPDGLCGNEDCGQMSAWYVFSALGFYPVLPGSDQYVLGTPL
                     FERAVIHLEDGTDFTLLAPDVSYENFYVSGMKRDGQDYPYSYIIYDDIFSGAEFEFEM
                     SEEPDEEFGSDPSVRPISAIEGDFVENPWVNVSNPMFLKSVEVSLGALNPDYRIWYSV
                     EPLDGEKTGTGSGDFKLYTGPFTLNESSRIRCYCENESWLRSHITESVVRKISSTYNV
                     TIKEKYSRQYSAGGDLGLVDGIRGSVNFRLGGWQGYQRKDFEAIIDLKEPRQVTKLAA
                     GFLQDIKSWIWMPRYVEFYTSMDGVTYRFAGRVGHSVADNDYTPQIHDLGVTITDDYG
                     NVGTGVEARYIKVFAKNYGKIPKWHLGVGGNAYIFTDEVIVK"
BASE COUNT          768 a          683 c          600 g          747 t
ORIGIN      
        1 agacgcgata tgacttatga aaagacggga ctcccatgga tactcccctc tcctcaggtg
       61 cctcaggcac agaatgcaat cggttatcct tgtgcaggca ttgttggcga cttttcaggc
      121 cattatctca atatcggcgt aggatacact cttcctttcc tgacatttgc tgcagagtgg
      181 gttgatgctg acgacctcaa agcagaactc gacagttaca acatcccggg agtagcctgg
      241 cgcaccattc attacaaacc cttctttggt tcaagtcagg ggaaactcct ccacggcgta
      301 caatatttct tcacagacta tgagatagct cctctgacat tgatcaattt ttatgtgatg
      361 caggcaatac acaaactcta tcccgatcat aatccctatg agggaggaaa aaacaggact
      421 actgatatcg tgtgcggtac tgactccatt cgccttaaat tcggcgaaaa tttcctcgta
      481 tccgatatga tcgactactg gaataaggat gtggagcatt tcagagaact ctcgaaaaaa
      541 tattatctgt acgagtagag agagtacaga tcaatgtaac cccaaaacaa taatgactta
      601 gcgttattaa ggggttcttt cagattggga caaagctcga tgttttatcg ggctttttcc
      661 ttttagtctt gctttgttgt tttttctctt actatacact ttcttgtgaa tccaaatcac
      721 ttataccttt ggatattgct tgttattctc taccttagag ttttgtatga aaaaagtcgt
      781 tgcattatat ttcagcccta cgggcgggac agcaaaggtg gtcagatcca tggcccgtgc
      841 ctttgctgct gctatgggct gtgattattc ggaaataaat gtaacactgc cggagcaaag
      901 aaagagtgcg cctgcttttg gagctgatga cgtagtattc ttcggggtcc cgacctatat
      961 cggaagggtg ccgaacctta ttagaccctt tttcgcctcg attaagggtt gcagagcaat
     1021 tgccgaaaca gttgaggaag cagttgctgg agtggttgac gtttcaagcg atgtggtgac
     1081 tggcggagct ctcgggatac cagtcgtaac ttatggcaat cgggcatacg atgatgctct
     1141 gatagaattg agggatatta tggaagaaaa tggctttaga accatcgctg cggcagcttt
     1201 cccctcggaa cactcctttt ccactaccct cgcggaaggt cgtccgaatg aaaaagactt
     1261 gcaattagcg aaggaatttg cgggaaaagt ggccgaaact attacgaaga atcctcaact
     1321 tatctccgat agccacactc caatagccgt accgggcaat cccgctccct actcctatta
     1381 caaagctgta atcagcgata acaaaagtat agatatccgc aaagtcaagc ccataacaga
     1441 ccctgagaaa tgcacaaaat gcggaatatg cgccctactc tgccctatgg gctccataaa
     1501 catttctgac ccatccatca ccgatggaat atgcatcaaa tgcggtgcct gcatcaagag
     1561 atgccctcag ggcgccaaat ccataaccga ccccgattac ctcgaacaca aacggctcct
     1621 cgaagaagaa tacggaaatg ttataaaaaa agcagagctt ttttacacta tttaacaatt
     1681 acttcgtcgg tgaagatgta ggcgttgccg cctacgccga ggtgccattt tgggatttta
     1741 ccgtaattct ttgcaaagac tttgatgtag cgcgcttcca ctccagtacc cacattgccg
     1801 tagtcatctg taatggttac cccaaggtcg tgaatctgag gtgtgtaatc attgtccgca
     1861 acactatggc ctaccctgcc ggcaaatctg tatgtgactc catccattga ggtataaaac
     1921 tccacatacc ggggcatcca tatccaggat ttgatatcct gtagaaatcc tgcagcgagt
     1981 tttgtcactt gtcggggctc tttcagatct atgatagctt cgaaatcctt tctctgatag
     2041 ccttgccaac cacccagacg gaaattgaca cttccgcgga taccatccac cagtcccagg
     2101 tctcctccgg cagaatattg tctgctgtat ttctctttaa ttgtaacatt gtaagtagaa
     2161 ctgatcttcc tgaccacaga ttcggtgata tggctgcgaa gccaggattc attttcacaa
     2221 taacagcgga tacgggagga ttcattcagg gtaaaaggtc ccgtatagag tttgaaatca
     2281 ccgcttccgg tgcctgtttt ttccccgtca agaggctcca cgctatacca aatccggtaa
     2341 tcgggattca atgcgccgag acttacctca acactcttca gaaacatcgg attggaaaca
     2401 ttcacccagg gattttccac aaaatcccct tcaatggccg aaatcggcct tacagatggg
     2461 tcagagccaa actcctcatc aggttcctca ctcatttcaa actcaaattc cgctcctgag
     2521 aaaatatcat cgtaaatgat atatgaatat ggataatcct gaccatctct tttcattcca
     2581 ctcacataaa aattctcata agagacatcg ggagccaaaa gggtaaagtc agtaccatct
     2641 tcgagatgta tgacggctct ctcaaataga ggtgttccca gcacatattg gtcactgccg
     2701 ggaagcacgg ggtaaaatcc caaagcgctg aaaacatacc aggctgacat ttggccgcaa
     2761 tcttcattgc cgcaaagtcc atcgggttcc gcggtaaa
//
LOCUS       DAOSIE010000199         2763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_31790_length_2763_cov_3.957164, whole genome shotgun sequence.
ACCESSION   DAOSIE010000199 DAOSIE010000000
VERSION     DAOSIE010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31790_length_2763_cov_3.957164"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PLI69_05860"
     CDS             <1..503
                     /locus_tag="PLI69_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG42044.1"
                     /translation="VFVKGTDINQLKDKELVNFRLHNIGFVFQLLNLIPVLTVEENIG
                     FVMELQGADKEQRIARTAELLELIGMKDRAGSRPSTLSGGQKQRVAVARALASKPLFI
                     LADEPTANLDTEAAETLLNTMLLLNEKEKITFLFSTHDPRVMNKARRLISLEDGRITK
                     DEGYVR"
     gene            466..1641
                     /locus_tag="PLI69_05865"
     CDS             466..1641
                     /locus_tag="PLI69_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42045.1"
                     /translation="MAGLQRMRDMFGRFIIAFSLFQLCFYSANAQVDLTGYINNYSLL
                     GETRPNPFDETLWQNMTSQRFNFNWRINNNFNIYISSRTNFSIGNSTILKMIKATSTD
                     DYNLMKLSYSLVDKDDKWLGVALDRLALRWAADDFEITLGRQRINWGNTILWNPNDIF
                     DSSPVINFTYPEKMGCDAVRATFYHSEVATSEIALSADRDGSPTAALFHRNNFRKLDY
                     QFLGGVYKGHYLFAGGGFTTELKHVNIRFEGSYFRDFRKVGDKRDIVQIAMGIDKIFS
                     NNLILQTEVLYTNKPYDISDVLFSYVPKRQSVRELTTSSWSFAGQLYYPFSNLFSINS
                     LVAYFHDQKAFLVGAEMSYQIFKNFELSLMVGAFDYTLGQSYSMDGLAGTLHLKYYF"
     gene            1673..>2763
                     /locus_tag="PLI69_05870"
     CDS             1673..>2763
                     /locus_tag="PLI69_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42046.1"
                     /translation="MKYSFLTLFIIAFSLLSCEREPLFEEIEYVKYSHQLFEENTRMG
                     ELIQKASREFGATPKEMLLKAGYSETQADQFLEKLNLYVALSKDYTVHGIAYNTKDPL
                     GESVVASGLLYCPQNRLPKGVIFVYPFFKSKGVSGTDTRYSVESLLAVAGDYVCIIPD
                     GIGMGINSDQPMSIIQHENLAETGIDFYLAVREFIYNRYHYKLPKRLILFGYSLGGSA
                     TWSLARYLSIHKELEIGVKDIFVGGGPYYPDLCMKDLFESRYSEFASGPFILWSLNYY
                     DNLNLDFTKIFKGKLLDGFPGLCNGSRAVHDVTSYIGTDLNDYFDEDFLQNEENQYRR
                     LVMDALKKHSIPNDWRPDVKVRLYNCHKDL"
BASE COUNT          811 a          529 c          585 g          838 t
ORIGIN      
        1 aagtttttgt aaagggaaca gatataaatc agcttaaaga taaagaactt gtaaattttc
       61 gtttgcataa cataggattt gtttttcaat tactcaatct gattccggta ttgacggtag
      121 aagagaatat aggatttgta atggagttgc agggggcaga caaagagcaa agaattgcgc
      181 ggacagccga actgcttgag ttgattggca tgaaagaccg tgccggctct cgtccatcca
      241 ctctttccgg tggtcaaaag caaagggttg cagtagcacg tgctttagcc tctaaacctc
      301 tctttattct tgcagatgaa cccacggcaa atctcgacac ggaggctgcc gaaactttgc
      361 ttaacacaat gctccttctt aacgaaaagg agaagatcac tttcctcttt tctacgcacg
      421 atcctcgtgt gatgaacaag gccagacgat tgatatccct tgaggatggc aggattacaa
      481 aggatgaggg atatgttcgg taggttcatc atagcatttt ctctgtttca gctctgtttt
      541 tacagcgcaa atgctcaagt cgacctgacg ggatacatca acaactactc actgcttgga
      601 gagaccaggc ctaatccttt tgatgagact ttatggcaaa atatgacctc tcaaagattt
      661 aatttcaatt ggaggatcaa caacaatttc aacatataca taagcagcag aacgaatttc
      721 tccattggaa acagcaccat cctaaaaatg ataaaagcca cttcgacgga tgattacaat
      781 ctgatgaaac tatcctattc actggtagac aaggatgata aatggttagg agttgctttg
      841 gaccggcttg cgttacggtg ggctgctgat gattttgaaa ttactctggg tcggcagagg
      901 attaactggg gaaatacaat attgtggaac cctaacgata tctttgactc ctcacctgtc
      961 attaatttca cttaccctga gaaaatgggc tgtgatgcag tgcgtgctac attttatcac
     1021 agtgaggttg ctaccagtga aattgctctg tctgccgaca gagatggttc ccctacggca
     1081 gctttatttc ataggaataa ttttagaaaa ttagattatc aatttttggg aggagtatat
     1141 aaggggcatt atctctttgc cggagggggc tttactaccg agttgaagca tgtcaatatc
     1201 cgatttgaag gatcatattt tcgcgatttc cgaaaggtag gtgataagcg tgacatcgtt
     1261 cagattgcta tgggtattga taaaatattt tcaaacaacc ttatcttaca aactgaagtc
     1321 ttatatacaa ataaaccata cgacatatcg gacgttctct tttcatacgt tcctaaaagg
     1381 caatccgtga gagagttgac tacctcttca tggagctttg caggacaact ttattatcct
     1441 ttttccaatt tgttctcaat aaactcctta gtggcatatt tccacgatca aaaagcattt
     1501 ttggttgggg cggaaatgag ctatcaaatt tttaaaaatt ttgaactatc tttgatggtt
     1561 ggtgcatttg attatacctt gggtcaatct tattcaatgg atgggcttgc aggcactcta
     1621 catttaaaat attatttttg aggtgcaata aaatagatta gtttacaaag ggatgaaata
     1681 ctcattttta acactattca ttatagcttt ctctctgtta agttgtgaga gagagcctct
     1741 atttgaggaa attgaatatg ttaaatacag tcatcaacta ttcgaagaga atacgaggat
     1801 gggagagctt attcagaagg cttcgagaga gtttggtgcc accccaaaag aaatgttgtt
     1861 aaaagcgggt tatagcgaga cgcaagcaga ccaatttctt gaaaaactca acctatatgt
     1921 tgcgctttca aaagattata ccgtacatgg tattgcatat aatactaaag atccccttgg
     1981 tgagtctgtc gtggcatcgg gactcctata ttgtccgcaa aacagattac ctaaaggcgt
     2041 aatcttcgtc tacccatttt tcaagagcaa aggggtaagc ggtactgaca ctcgatacag
     2101 tgtagaatct ctcttagctg tggcaggtga ttatgtatgt attataccgg atggtattgg
     2161 catgggcatc aattcggatc aaccgatgtc tattatccag catgaaaatt tagccgaaac
     2221 aggtatagat ttttatctcg cagtcagaga gttcatttat aatcgctatc actataagct
     2281 ccctaaacgg cttattctgt ttggctattc cctcggcggt tccgctacct ggtcgttggc
     2341 aagatatctc tccatccata aagagttgga aatcggcgtt aaagatatct ttgtcggagg
     2401 aggcccttat tatcctgacc tctgtatgaa ggatttattt gaaagccgct attcggaatt
     2461 tgcttcaggc ccctttatac tctggtcttt aaactactat gacaatttaa atctggattt
     2521 taccaagatt ttcaagggca aactattgga tggatttccc ggattgtgca atggctccag
     2581 agcggtgcat gatgtgacct cctatatcgg aactgatctt aacgactatt tcgatgagga
     2641 ttttttacaa aacgaagaga atcaataccg ccggttggtt atggatgcac tgaaaaaaca
     2701 ctctatacca aatgattggc gacctgacgt caaagttcgt ttatacaact gccataaaga
     2761 cct
//
LOCUS       DAOSIE010000200         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_31987_length_2750_cov_2.716141, whole genome shotgun sequence.
ACCESSION   DAOSIE010000200 DAOSIE010000000
VERSION     DAOSIE010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31987_length_2750_cov_2.716141"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLI69_05875"
     CDS             <1..310
                     /locus_tag="PLI69_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42047.1"
                     /translation="DALTYKSNCTYDKTAVFSEIYYPYGWKAYIDDVPVEHFRVNYLL
                     RALNVPAGVHEIRFEFRPDSVIRGNMISALFTFVMVTIIIASMLFPCLRSCRNKVEKE
                     "
     gene            322..1821
                     /locus_tag="PLI69_05880"
     CDS             322..1821
                     /locus_tag="PLI69_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG42048.1"
                     /translation="MGIIIRQSLKGTLANYLGVAIGFFTTFFVLVRYLTPEEIGLTRV
                     LLDAAVLLAGLSQLGTSSSVMRFFPYFKDEKNKDNGFFFWTLIIPLVGFAIFTLLFFI
                     FKRPVCDFFQEKSPLFVDYYHLVIPIAFWILYNTVFESNASVLERIAVPRFIKEVGCR
                     IFLLILYLLYGFKVISFERVLEGFAVIYFLCTLLSIGYLISLKRVSFKPNFKYVTPQI
                     RKDFTLYTLFLVTAAITGLITPSINTFFVTAKMGLESTGVLTIAIYIVAIIEMPYRSL
                     GVVSLPSISQAMKDGDLQRTNELCKSVSLHQLLISTFIFMVIWMNIDLFFELLPNGDK
                     YSDAKMIVAILGLSRVLHSSLSIATSALGFSKHYYFSLIFTFVLTFTAIVLNNKLIPI
                     YGIAGAAWATLISYLVAFLFLLPFLYFTTKVSILSKGQIIVFFIFIIMLILEIFWNRL
                     FEMPSSLIPKIAFYGVKTLVICGLGIVAVYLNKVSADVNKIIDKILRRG"
     gene            complement(1824..>2750)
                     /locus_tag="PLI69_05885"
     CDS             complement(1824..>2750)
                     /locus_tag="PLI69_05885"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42049.1"
                     /translation="KSFKVIFENNLPHNIKYRHKVITDIWDESPLGEDGKIAIADSFF
                     INRRKSKPAGDKVYTAHQKFGLLPKDWNNSKHNIAIFNSSEDEFAAVGGDFDKLALFD
                     SQLEGLTYILDNFIDNKDYHFYLRVHPNLSKIAYKYHTDLYKLSERYQNVTVIPATDR
                     IDTYALMDAAEKVVVFGSTMGVEAAYWKKPVILLGASLYYYADICHTPKTKEEIVPLI
                     KSALEPKENLFIRKFGYYIMDFKRATFEEQMYTYFDYTTFSISIGGLKAHGVNYQKLL
                     GSKKFFLFTVSAIRLILGKLFKDKFKLPVEEE"
BASE COUNT          734 a          506 c          552 g          958 t
ORIGIN      
        1 tgatgcactg acctacaaga gcaattgcac atacgacaaa accgcagtat tctctgaaat
       61 atattacccc tatggctgga aggcctacat tgatgatgtc ccggtagaac atttccgtgt
      121 aaactatctc ctcagggcac ttaacgttcc ggcaggcgtc catgagattc gctttgaatt
      181 ccgccctgat tcggtgataa gaggcaatat gatctctgcg ctctttactt ttgtgatggt
      241 aacaattatt atcgcttcga tgctatttcc ttgtttgaga agttgtagaa acaaagtaga
      301 aaaagaataa ttataattat aatgggaatt attatcagac aaagtcttaa aggaactttg
      361 gcgaactatc ttggtgttgc cattggtttc tttactactt ttttcgttct ggtacgttat
      421 ctgactcctg aagagattgg tctgacaaga gtattactgg atgcggcagt actgcttgcc
      481 ggtctttcgc aactgggtac tagctcttcc gtaatgagat tttttcccta tttcaaggat
      541 gaaaagaata aagacaatgg attctttttc tggaccttga ttattccgct ggtcggtttt
      601 gccatcttta ctctcttgtt cttcatattc aagagaccgg tgtgtgactt cttccaggaa
      661 aaatcacctt tgtttgtgga ttactaccat ctggtgatcc cgatagcatt ttggatactt
      721 tataatacag tgtttgaaag caatgccagt gtgttggaaa gaatcgcggt gccgcgattc
      781 ataaaagagg tcggttgcag gatttttttg cttatccttt atcttttgta tggattcaaa
      841 gtaatcagtt ttgaaagggt actggaaggc tttgcagtca tctatttttt gtgtacatta
      901 ttgagtatcg ggtatcttat atcattgaag agagtctcct tcaaacccaa tttcaaatat
      961 gtgacaccac aaatccgtaa agactttaca ttatatacgc tctttttggt aacggctgct
     1021 attacaggtt tgattacgcc gagcattaat accttttttg taacggcaaa gatgggactg
     1081 gagtcaacgg gagttctgac cattgcaata tatattgtag cgattatcga gatgccctat
     1141 cgatctttgg gtgtagtttc acttccttcc atatctcaag ccatgaagga tggggattta
     1201 cagcgtacaa atgagttgtg caagagcgtt tccctacatc aattactcat atctaccttt
     1261 atttttatgg tgatatggat gaatatagac cttttctttg agttactccc taatggagac
     1321 aaatattccg atgcaaaaat gattgtagct attctcggat tgtcgagagt ccttcattct
     1381 tcactaagta tagccacttc tgcgttggga ttttctaaac attactattt ctctctaatc
     1441 ttcacctttg ttctgacttt tacggcaatt gtgttgaaca acaagttgat tccaatttat
     1501 ggaattgccg gcgctgcgtg ggctactcta atatcttatc tcgtagcttt tttatttctt
     1561 ctgccttttc tttactttac cacaaaagtc tcaattctct ctaaaggaca gataatagta
     1621 ttttttattt ttattatcat gctgatcttg gagatatttt ggaataggtt gtttgaaatg
     1681 ccttcttcac ttattccaaa aattgccttt tatggtgtga aaacacttgt tatctgtggt
     1741 ttgggaattg tcgccgttta cctgaataaa gtctcggcgg atgtaaacaa aattatagat
     1801 aaaatcctca ggagggggta gaattattcc tcttccaccg gcaacttaaa tttatcttta
     1861 aatagttttc ccaatatcag tcttattgca gatacggtga aaagaaaaaa ctttttagag
     1921 cctagaagtt tctgataatt gactccatgt gctttgagtc cgccgatcga gatcgagaag
     1981 gtagtgtagt caaagtatgt gtacatctgt tcttcaaatg tggctctctt gaagtccatg
     2041 atgtagtatc cgaatttccg gataaatagg ttctcttttg gctcaagtgc agatttaatt
     2101 agcggtacga tctcctcctt agttttcgga gtatggcaga tatccgcata gtagtatagt
     2161 gatgctccga gcagaataac cggttttttc caatatgcag cctcgactcc catagtggaa
     2221 ccgaatacta cgaccttttc agcagcatcc ataagtgcgt aggtgtcaat tctatcagtt
     2281 gcaggtatga ctgtaacgtt ttgataccgt tcacttaatt tgtataagtc agtatggtat
     2341 ttatatgcta tttttgaaag gtttgggtgt actctgaggt aaaagtgata atccttgttg
     2401 tctatgaagt tatcaagaat gtatgtaaga ccctctaatt gtgagtcaaa aagggcaagt
     2461 ttgtcaaaat caccacccac ggcagcaaat tcatcttcgg atgaattgaa tattgcaata
     2521 ttgtgtttgg aattgttcca gtctttggga aggagtccga atttctgatg ggcagtatag
     2581 actttatccc cagcaggttt acttttccgc ctattgataa agaaggaatc agcaattgct
     2641 attttcccat cttcgccaag aggggattca tcccatatgt cagtaataac tttgtgtctg
     2701 tattttatgt tgtgtggtag attattctca aaaatcactt tgaaagactt
//
LOCUS       DAOSIE010000201         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_32021_length_2747_cov_3.995171, whole genome shotgun sequence.
ACCESSION   DAOSIE010000201 DAOSIE010000000
VERSION     DAOSIE010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32021_length_2747_cov_3.995171"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..2538
                     /locus_tag="PLI69_05890"
     CDS             172..2538
                     /locus_tag="PLI69_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG42050.1"
                     /translation="MKGRIEQMFEELNPAQKEAVSNIEGPSLIVAGAGSGKTRVLTCR
                     IANILSQGYDPSRVLALTFTKKAAGEMKERIAAIVGERSARRLWMGTFHSIFIRFLRE
                     YSQQLEYPREFTIYDQSDSRNAIKQCIKELQLDDKIYKPAQIQSRISLAKNNLITVSG
                     YYADASLMEADAASRRGRIADIYALYVKKCKTAGAMDFDDILLNTNILLKNFPEAREA
                     ISAKFDYILVDEYQDTNHAQYIILKKLAEPHRNICVVGDDSQSIYGFRGARIENILKF
                     KRDYPDATEYKLEQNYRSTKTIVKAANSVIANNSNRLKKECFSEGAEGEKIGIIKAFT
                     EQEEAYLTASSIMRRIFSDKAQYGDFAILYRTNAQSRVLEEALRKRNLPYRIYSGQSF
                     YDRAEVKDMLAYFRLIVNPRDDEAFRRVINVPARGIGSTSLSRLSDAATETGSSLYEA
                     IFLPPEKFIEVGLKPAAVNKFLNFVTMMEPIHQEWSTTDAFTIAQRIGNDSGYLASLK
                     NDNSPEGISRFENIEELYNSIKSFVDEERETRAMENDSPEEEIIVTLDDYIENISLMS
                     EMDREEEEMDEAEVRNKITLMTVHSSKGLEFPYVYIAGMEENLFPSPGLNGSDMDIEE
                     ERRLFYVALTRAQKCATISFARNRMRWGQQESNPVSRFVREIDSRHLEMPIDDQPRMP
                     YMREEFATPAERVGGRQESMQNRPDVLRRYELTKSFSRGSKHTPSEGFQPSAIYDLKV
                     GQRVEHATFGFGTIISFDSNDANAKAVVKFDNSGEKTLLLKFAKLRIV"
BASE COUNT          841 a          591 c          626 g          689 t
ORIGIN      
        1 taaatccagt cccttcttca atctgatttg ttctgacatt ctttatttca tattattgat
       61 ttaacactta agtatttaca caatcagccg caaagttatg tataaaaaac gatattcaat
      121 cctttgaaat gcaatttctc gggcttattt ttgcataatt ataaattatt tatgaaaggt
      181 aggatagaac aaatgtttga agagctgaat ccggcacaga aagaggctgt cagcaatata
      241 gaagggcctt cacttatcgt tgcgggtgcc ggttcaggca aaaccagggt gcttacctgt
      301 agaattgcca atattctctc tcaaggatac gatccctccc gagtgcttgc tttgacattc
      361 accaaaaagg ctgccggaga aatgaaggaa aggatagcgg ccattgtagg tgaaaggagc
      421 gcaagaaggc tctggatggg aactttccac tcaatattca taagattctt aagagaatat
      481 tcgcagcaac tcgaatatcc gcgggaattt accatatacg atcagagtga ctccagaaat
      541 gccatcaaac aatgtataaa agagcttcag cttgacgaca aaatctacaa gccggctcaa
      601 atacaatcca ggatttcact ggccaaaaac aatcttataa cggtaagtgg ctattatgca
      661 gatgcatctt tgatggaagc cgatgccgca tcgaggagag gccgtatagc cgatatttat
      721 gcgctatacg tcaaaaaatg caaaacagca ggagcaatgg atttcgacga catcctccta
      781 aataccaata ttctgctgaa aaattttcct gaagcgagag aggctatctc agccaaattc
      841 gactatatcc ttgtggacga ataccaggat accaaccatg cgcaatacat cattctcaaa
      901 aaacttgctg agccacacag aaatatctgc gtagtgggtg atgactctca gagtatctac
      961 ggatttcgcg gagcgagaat agagaacata ctcaaattca aaagggacta ccccgatgca
     1021 accgaataca aactggaaca gaactaccgc tcgacaaaga ccatcgtcaa ggcggcaaac
     1081 agcgtgattg ccaataatag caaccgcctc aagaaagagt gcttctcaga gggtgcggaa
     1141 ggtgaaaaaa tcggtatcat aaaggccttc accgagcagg aagaagccta ccttactgca
     1201 tcctccataa tgcggcgaat ctttagcgac aaggcgcagt atggggactt tgccatcctc
     1261 taccgtacaa acgcccaatc aagggtgctg gaagaggccc tacgcaaaag aaacctcccc
     1321 taccgcatct attccggcca atctttctac gatcgtgccg aggttaagga catgttggca
     1381 tactttcgtt tgatagtcaa tccacgcgat gacgaagcct tccgcagagt aatcaatgtg
     1441 cctgcccgcg gaattggaag tacctcccta agtcgcttgt cagatgcagc aacggaaacc
     1501 ggttcttcgc tctatgaggc tattttcctc ccccctgaga aatttattga agtaggactc
     1561 aagccggcgg cagtcaataa atttctgaac ttcgtcacca tgatggagcc gattcatcag
     1621 gagtggagta ccactgatgc atttaccata gcacaacgta taggtaatga ttcaggctac
     1681 ttggcttcac ttaaaaatga caactctccg gaaggaattt ccagatttga gaatatcgaa
     1741 gaactctata atagcatcaa aagtttcgtg gatgaagaga gggagacaag ggcaatggag
     1801 aacgactccc cggaagagga gataatcgta acccttgacg actatattga aaatatctcg
     1861 ctgatgtctg aaatggatcg tgaggaagag gagatggatg aggcggaggt cagaaacaag
     1921 attaccctga tgacggttca ttcatccaag ggactggagt ttccctatgt ctacatagcg
     1981 gggatggagg aaaacctctt cccttctcca ggtttgaatg gaagtgatat ggatatagag
     2041 gaggagcgca ggctattcta tgtggccttg acaagggctc agaagtgtgc aacgatcagt
     2101 tttgcccgca acaggatgcg ctggggccag caagagagca atccggtcag ccgtttcgtc
     2161 agagagatcg acagtcgcca cctcgagatg cccattgatg atcaaccgcg tatgccatat
     2221 atgagagagg agtttgctac acctgcggaa agggtaggcg gcagacaaga aagcatgcag
     2281 aataggcctg acgtcttgcg cagatatgag ttaacgaaga gtttctcccg ggggagcaaa
     2341 catacaccat cggaaggatt ccagccctca gccatatacg acctgaaagt gggacagagg
     2401 gttgaacatg ccactttcgg ctttggcacc attatatcct ttgacagtaa tgatgccaat
     2461 gccaaagcag ttgtaaagtt cgacaattca ggtgagaaga ctcttttgct taaatttgcg
     2521 aaacttcgca ttgtttgata tttttagtaa aatttgtcaa actttgaata ctatttttaa
     2581 actttggcac atagattgca tatacttatt tcgaagtttt tcatagttta agtttaggtt
     2641 aagtaatgcg gtttgtttcc aatctgacgg agatgagccg catgaatttt ataccgctgc
     2701 tatatccatt ctgacatttt ttcttaattt tgagaatcaa acagaca
//
LOCUS       DAOSIE010000202         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_32288_length_2731_cov_3.034006, whole genome shotgun sequence.
ACCESSION   DAOSIE010000202 DAOSIE010000000
VERSION     DAOSIE010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32288_length_2731_cov_3.034006"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>726
                     /locus_tag="PLI69_05895"
     CDS             <1..>726
                     /locus_tag="PLI69_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOG42051.1"
                     /translation="LQEGVITPSSTYSCYGAYTVGNLRVGCHGHPSPLDFRRSIMMSC
                     NTYYCYVFRAILDNPKYKNIRESFTIWKEYAESFGFGMKLQTDIPSEQAGTMPTAEWY
                     DRLHGKNRWRSLSIISLAIGQGEIGSTPLHLANLAAIIANRGWFYTPHLQKDMPDNPI
                     DSRFKELHYTMVDTTWFKYVVDGMYLAVNGGPGATGRLANVPGLDVCGKTGTAQNPHG
                     NDHSIFICFAPKENPRIAVAAYI"
     assembly_gap    727..736
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            900..2459
                     /gene="rodA"
                     /locus_tag="PLI69_05900"
     CDS             900..2459
                     /gene="rodA"
                     /locus_tag="PLI69_05900"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOG42052.1"
                     /translation="MAKSIYRKLDWTIITCYLALVLFGWINIYSSLYQEGAVIFDFSM
                     EYGKQIIWIGTSVVLALLILFAIPPKIYTAFAWWLYALMAVLLVAVIFIGANVKGSNS
                     WLQIGSMRVQPAEFSKITTSLALAHLMSSKFDFSFKNKSDALKAFAVFLLPMSAIILE
                     KETGTALVYLGFLLMFYREGMSGWILIFGLSVIGIFISTLVFSPYISIVIVVGLVTLF
                     AALVTRKKWVVAVVLPLIVLLGFIPSLISDIGSMEKPATEIVSVEIDRDNSADESATI
                     ADDDMVSAASESPGKFAIALRKFFSRFPAEIWLVFALAPFLLVLLIKSVLKRQKILRN
                     TILSMIVGISLVFSVDFIFDNVLKDYQKGRIEVLLGIKEDPMGDGYNVHQSMIAIGSG
                     GFFGKGFLRGTQTRFDFVPEQTTDFIFCTIGEEWGFVGSLAVLAIFLVLLIRLINSAE
                     KQRDRFIRIYGYCVASILFMHILINVGMTVGLMPVIGIPLPFVSYGGSSLWGFTILLF
                     IYVRLDLERWR"
     gene            2528..>2731
                     /locus_tag="PLI69_05905"
     CDS             2528..>2731
                     /locus_tag="PLI69_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42053.1"
                     /translation="MPHKFVDRHNGPRDFQEKIMLERLGVSSIDELIKQTVPSDILLK
                     EPLALEPALSEHEYTARLKKTASL"
BASE COUNT          718 a          583 c          649 g          771 t
ORIGIN      
        1 gacttcagga aggggtaatc actccctcat ccacctattc ttgctatggt gcttatacgg
       61 tcggcaacct cagagtagga tgtcacgggc acccctctcc cctcgatttc agacgctcga
      121 taatgatgtc ctgcaataca tactactgct atgtattccg tgcgatcctc gacaatccga
      181 aatataaaaa catccgcgaa tcctttacaa tctggaaaga atatgccgaa agtttcggat
      241 tcggaatgaa attacaaacc gatatccctt ctgaacaggc aggtacgatg cccactgccg
      301 aatggtacga cagacttcac ggcaagaacc gctggagatc actctcaatc atctctctcg
      361 ccataggtca gggtgagatt ggctccacac cgctgcatct ggcaaacctc gcagccataa
      421 ttgccaaccg cggctggttt tacacgccgc acctacaaaa agatatgccg gacaatccta
      481 ttgactcccg cttcaaagaa cttcattaca cgatggtgga taccacctgg tttaagtatg
      541 tggtggatgg catgtatctg gccgttaatg gtggccccgg agctaccgga aggctggcaa
      601 acgttcccgg acttgatgta tgcggaaaaa ccggcacagc tcaaaatccg cacggcaacg
      661 accactcaat attcatctgc tttgccccga aggagaatcc caggattgcg gttgcggcat
      721 atattgnnnn nnnnnntatt gagaatgccg gtttcggaag cagttgggcg gccccgattt
      781 catcccttct tgtggagaaa tacctcaacg gtgagatctc ccccgggaga aaatggcttg
      841 aaactaatat gataaacgga tacctgcttg atagggtaac agtaaggagg aggtagagag
      901 tggcaaaatc aatctataga aaactcgatt ggactataat cacctgctat ctggcattgg
      961 tgcttttcgg gtggattaat atctattcgt ctctatatca agagggggca gtcatttttg
     1021 acttctcaat ggaatatggt aagcagatta tatggatcgg gacttcggta gtgctggcgt
     1081 tgctaatact atttgccata cctccaaaaa tctatactgc ctttgcctgg tggctgtatg
     1141 cgttaatggc ggtgctactg gtggcggtta tatttatcgg agccaatgta aaaggttcca
     1201 atagttggtt gcagatagga agcatgaggg tgcagccggc cgagttttcc aaaattacca
     1261 cctcgctggc ccttgcccat cttatgagca gcaaatttga tttcagtttt aagaacaaat
     1321 cagatgccct taaagcattc gcggtcttct tgttgccgat gtcggcaata attttggaga
     1381 aggagacggg aaccgctctg gtttatcttg gatttctgtt gatgttttac cgtgagggaa
     1441 tgtcgggatg gatacttatt tttggattat ccgtaattgg tatttttata agtaccctgg
     1501 tcttttcacc ctatatttct attgtaatag tggtaggatt ggtaactcta tttgcagcgc
     1561 ttgtaacgcg aaaaaagtgg gtagttgcgg tcgttttgcc attaatagtg ttgctgggat
     1621 ttattccttc gctcatcagc gatataggta gtatggaaaa gccggctaca gaaatagtct
     1681 cagtggaaat agacagagac aatagtgcag acgagtccgc tactattgcg gatgatgata
     1741 tggtttccgc cgcaagcgag agtccgggta aatttgccat tgccctcaga aaatttttca
     1801 gcaggttccc tgcggaaatt tggttggtgt tcgcccttgc gcccttcctg cttgtgcttc
     1861 tgatcaagtc agtgttaaaa cggcaaaaga tattacgcaa cacgatactt agtatgatag
     1921 tgggcatctc tttggtcttt tcggtggatt tcatatttga caatgttctc aaagattacc
     1981 aaaaaggacg tatagaggtg cttctgggaa tcaaggagga tcctatgggc gatggttaca
     2041 atgtccacca atccatgatt gcgatcggtt ccggaggatt tttcggtaaa ggatttctcc
     2101 ggggtacaca gactcgattt gattttgttc cggagcagac taccgatttt attttttgca
     2161 ccataggaga ggaatggggt ttcgtagggt ctttggcggt actggccatt ttcttggtat
     2221 tgctgataag gcttatcaat tccgcagaaa aacaaaggga ccgttttatt cgtatttatg
     2281 gctattgtgt cgcctccatc ctatttatgc atattctaat aaatgttgga atgacggtcg
     2341 gacttatgcc tgtgatagga attccgctgc cctttgtgag ttatggaggc agtagccttt
     2401 ggggattcac cattttgcta tttatctatg tacgtcttga tctggagaga tggagataag
     2461 tccgattgga aaagaaataa taactgacta cctttgttga atgacaacta ttaaaaccat
     2521 ataaaacatg ccacataaat tcgttgacag acataacggg ccaagggatt tccaagaaaa
     2581 aatcatgttg gaaaggctcg gcgtatcatc aattgatgaa cttataaaac aaaccgtacc
     2641 gtcagacatt ctcctaaaag agcctcttgc tctggaaccg gctctctccg agcacgaata
     2701 cacggcacga ctcaaaaaga cggcatccct c
//
LOCUS       DAOSIE010000203         2720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_32456_length_2720_cov_4.472045, whole genome shotgun sequence.
ACCESSION   DAOSIE010000203 DAOSIE010000000
VERSION     DAOSIE010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32456_length_2720_cov_4.472045"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1733)
                     /locus_tag="PLI69_05910"
     CDS             complement(102..1733)
                     /locus_tag="PLI69_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG42054.1"
                     /translation="MKTTLSFTRSITRGFRLRIALIVVLGLFGVFFSLAFVWCSKVLI
                     DIATKAREGDLTFYALLLILLMAFNILFRVMDTRLRKMTEVKLGNAIRYNLFSQLLYT
                     RWQELSLLHSGDMLTRFIKDSDEVVNVLVTSIPISIVSLVQFSGAFVVLYFLDPTLAI
                     ILGLFLPIISLLGKSYYRLMHKYTLEVKESESQITSMVEENLQNQPVIRTFERQESEL
                     ERLQDLQLRLHGSVGKRTGVSVVANLILGVAFNGGYLTSFLWGAYGLAKGTVTFGIMT
                     AYLQLVARLQRPLYDLLRLLPTLVTAKASMERLIYLTGFAKEASGESIPLQGNVTLHV
                     DNLTFSYKEGDKPVLQEFSMSAGPGTMVALTGKTGEGKTTFLRLLLGLVKPDSGTLRI
                     ADESVSLEISEQTRANFVYVPQGGSLFSGTIRENLLLGDPEADDATLRRVLAIAAADF
                     VFDLPEGLETVLGQDGGGLSEGQGQRIAIARALLRKGKVLLFDEATSALDIETEKRFL
                     NNLKAGLQDRVAIFITHSEEVAAFCDYRYHLSSES"
     gene            1854..>2720
                     /locus_tag="PLI69_05915"
     CDS             1854..>2720
                     /locus_tag="PLI69_05915"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOG42055.1"
                     /translation="MSTKATEHPSNPYFLQLLSAAIWERPLDETLFEGLDKSLWREIA
                     SMAYRQSVSALVGDKAVSLPAESLPPGDITMRFISVIEQTRQTNLKMIGVLKELKEEY
                     TREGFPFLLLKGLANGVNYPEPLLRNPGDIDLLLYNKEDYDRLYGWLTQNGVDIQPEG
                     DLHFSFILNGIHVENHYRTTYFTNRWYSRRFVRLERNVLNKGEFAAAEIDSYLSVSVL
                     PVTFNALFLFHHLFRHFVSSGIGLRHVCDWILFLHKHREDIDGDEFTRLARRFALLYP
                     MQLFARVAIDYLG"
BASE COUNT          720 a          696 c          636 g          668 t
ORIGIN      
        1 cattaagaga gtagcacccc gcaccctgct cctcagcctc tgaatcctgt agttagatcc
       61 ctgtacctcg cccccaaatc ctgaatcccg caactcgcaa ctcacgactc ggaactaaga
      121 tgatatcggt agtcgcagaa ggcggccacc tcttcgctgt gggtgataaa aatagcaacc
      181 ctatcctgaa gtccggcttt taagttgttt agaaatcgtt tttcggtctc aatatcgaga
      241 gccgaagtcg cttcatcaaa cagcagcacc tttcctttgc gcagcagcgc gcgtgcaatg
      301 gctatgcgtt gcccctgtcc ctccgaaagt ccgccgccat cctgtcccaa aacagtctcc
      361 agcccctccg gcagatcgaa aacaaaatcg gcggcagcga tcgcgagcac ccgtctcaaa
      421 gttgcatcat cggcctccgg atcacccaaa agcaggtttt cacgtatggt tccgctaaag
      481 agtgaaccgc cttgaggtac atatacaaaa tttgcgcgtg tctgttccga aatctcaagc
      541 gaaacactct catctgctat gcgtagagtg ccgctgtcag gcttcaccag cccaagcagg
      601 agcctcaaaa aggtggtttt accctcaccc gtctttccgg taagggctac catcgtaccc
      661 ggcccggcac tcatggaaaa ctcctgcaag accggtttat caccctcctt gtaactgaaa
      721 gtcaaattat ctacatggag agtaacattt ccctgcaaag ggatggactc cccactcgct
      781 tctttggcaa aaccggtaag atagatcaac ctctccatgg atgcctttgc cgtaaccagg
      841 gtgggaagca gccgcaatag gtcatacaaa gggcgttgga gtcttgctac cagttgcaaa
      901 tatgccgtca taataccaaa agtaactgtg cctttcgcca atccgtaggc gccccagaga
      961 aatgatgtga gataacctcc gttgaaggcg acacccagta tcaggttggc aactaccgaa
     1021 acaccggtac gcttacccac gcttccatgc agcctcaatt gcaaatcctg cagccgttcc
     1081 aattcactct cctggcgttc aaaggtgcgt atcaccggct ggttttgcag gttctcctcc
     1141 accatcgaag taatttggct ttcactctcc ttcacttcga gggtgtattt gtgcatcagt
     1201 cgatagtagc ttttgcctaa cagggatata atgggtaaaa ataaacccag gataatggcc
     1261 agtgtggggt ctaaaaaata gagtaccaca aaggcacctg aaaattgcac gagggagacg
     1321 atactgatgg gaatcgaggt taccaataca ttcaccactt cgtcgctatc tttgataaat
     1381 ctcgtaagca tatcaccgct atgcagcaat gagagctcct gccagcgtgt gtagagcaac
     1441 tgcgaaaaga gattgtatcg gatggcattc cccagcttta cctcagtcat tttacgcagc
     1501 cgggtatcca tgacacggaa tagtatgttg aaagccatca gcaggataag caacagggcg
     1561 taaaatgtca gatctccctc ccgtgccttg gtggcaatat ctatcaacac tttggagcac
     1621 cagacgaaag cgagtgaaaa gaagacccca aacagtccca gcaccacaat gagcgctatc
     1681 cgaagccgaa atccccgggt aatggagcgg gtaaaagaga gagttgtctt cataaaaagt
     1741 ggaaaaaagt accaattatt atacaaaggt aataaaaatg atctaagcgg actttttatc
     1801 attcgctaaa aggagttatc tttgtgaata atttgtagaa aaactatttt acgatgtcga
     1861 ccaaggccac agagcaccct tccaacccct actttctgca gctgctgagt gccgctattt
     1921 gggagcgtcc gctcgatgag actctttttg agggattgga caagtcgctt tggagagaaa
     1981 tagcctctat ggcctaccgt cagagcgtaa gtgcgcttgt cggcgataag gcagtgtcac
     2041 tgccggcgga gtcccttccc ccgggcgata tcacgatgcg gtttatctct gtgatagagc
     2101 agaccaggca gaccaatctc aaaatgatcg gtgtgctgaa agagctgaag gaggagtaca
     2161 cgcgtgaggg ctttcctttt ctattgctga aagggttggc caacggagta aactatcctg
     2221 agcctttatt gaggaatccg ggcgatatcg acttgttgtt atacaataaa gaagactatg
     2281 accggctata tgggtggttg acccaaaatg gcgttgacat tcaaccggaa ggggatcttc
     2341 acttcagctt catactaaat ggcatccatg tggaaaacca ctatcgtacc acctacttca
     2401 caaatagatg gtacagcagg cggttcgtcc ggcttgaaag gaatgttctt aataaagggg
     2461 aattcgctgc cgcagagata gatagctatt tgtcggtatc ggtacttccg gtgaccttca
     2521 atgccttgtt cttgtttcac catctgttcc ggcatttcgt cagttccggt atcggacttc
     2581 gacacgtgtg tgactggatc ctatttctgc acaaacacag ggaggatata gatggagatg
     2641 agtttacccg tcttgccagg cgtttcgccc tactttatcc catgcagcta ttcgctcgtg
     2701 ttgcaattga ctatttgggt
//
LOCUS       DAOSIE010000204         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_32691_length_2704_cov_3.406569, whole genome shotgun sequence.
ACCESSION   DAOSIE010000204 DAOSIE010000000
VERSION     DAOSIE010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32691_length_2704_cov_3.406569"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2426
                     /locus_tag="PLI69_05920"
     CDS             <1..2426
                     /locus_tag="PLI69_05920"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOG42056.1"
                     /translation="FIRDLDLTVDNRDGMITTIVRSGMLQSGKVKGYDAIIKALVKDN
                     AIGLNLGFNNGEEAATYADLNARVSFPEPKENGLNIIADFYPSNIVINGENWTLNPSS
                     IGYREKDISVAGFGLSNGDQTIRFDGRLSANPEDTLLVTLNRFDASVANPLLDIPLDI
                     KGFLTGKAEFFGLFSDNKGILLNIDGEKMSIAGDQIGDLGIHTKWNETAKSFDFLVNN
                     TKNELTPLHLAGSYYPNKKSVDGNMSLDKFSLVCLNPVVEGLLSDIRGNISADIDISG
                     TLDKPVLKSTNGHFEDFNFILDYTKVPYTVNGPFSISENGVVFENAIVTDRFGHEGTL
                     TGGLQYDHFKDMRLNARLNAEHLHGLNTTAADNDSYYGRAFATGTVRLSGPFRKLLLD
                     MNITTGRNSTIHIPLGSSSSSHTSLLTFVDNTTVKSISAYDSLQVMNTIAEKRGGYEF
                     AVRMRVNATPDAEVHLEINKALGDVLKTRGSGQLVINAATDLFDIKGNYNIDEGSYKF
                     VMLGLASRDFIINPGGTINFTGDVMKSDLNLEAIYRTKASIGTLIADTTSISTRRTVD
                     CGIGITGKLANPELNFSIDIPDLDPTIKGRVESALSTEDKRLKQLLTLLLSGSFVPDE
                     QSGIVNNTTVLYSNASEIMANQLNNVFRQLNIPLDLGFNYQPGEKGRDIFDVAVSTQL
                     FNNRVNINGNIGNRQYMTSNQSDLVGDVDVEIKIDRTGRVRLTLFSHSADQYSNYLDQ
                     LQRNGIGLSYQEEFDDFRELFRKIFWSKRRRDEYAAQQARVLEIRQKDPLQRIQEQKS
                     KQTQETTIR"
     gene            complement(2394..>2704)
                     /locus_tag="PLI69_05925"
     CDS             complement(2394..>2704)
                     /locus_tag="PLI69_05925"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOG42057.1"
                     /translation="SILFIEDIDEKMHTVDRMLLTMRCAGMLDRLKGVIIGQFTDTAD
                     SDKWEKDVYTLIKEHTDRLDCPVLFGFPCGHEQPNYSLVLGREAVLKVSPDGGFLRLL
                     "
BASE COUNT          823 a          672 c          563 g          646 t
ORIGIN      
        1 atttcataag agatcttgac ctgactgtag ataaccgtga cggcatgatc accaccatcg
       61 tcagatccgg aatgctgcaa tccggcaaag tcaaaggtta tgatgcaatc ataaaagctc
      121 tggtaaaaga taacgccata ggacttaatc tcggatttaa caacggagaa gaggcggcca
      181 catacgctga cctcaatgcc agagtatcat tccctgagcc aaaggagaat ggattgaata
      241 ttattgccga cttttatcca tccaatatcg tcattaatgg agaaaactgg accctaaacc
      301 cctcttcaat aggctacaga gaaaaagata tatcggtagc cggcttcgga ctctccaatg
      361 gagatcagac tatccgtttt gacggacgac tatcggcaaa tcctgaagac acacttctgg
      421 tgacattgaa ccgttttgat gcatccgtgg ccaatcccct tctcgatata ccgcttgaca
      481 taaagggatt tctgaccggt aaggccgagt ttttcggcct cttttccgac aacaagggca
      541 tattgcttaa tattgatgga gaaaaaatgt caatcgccgg agatcaaatc ggggaccttg
      601 gtatccacac caaatggaat gaaaccgcca aatcttttga ttttctggtc aacaacacaa
      661 aaaatgaact cacaccccta catcttgcag gatcttatta tccaaacaaa aagagcgtag
      721 atggaaacat gtcattggac aaattctccc ttgtatgtct aaaccctgta gtagaagggc
      781 tgctatccga tatccgcgga aacatttctg cagatattga catatcaggg acactggaca
      841 aaccggttct aaaaagtacc aacgggcatt ttgaagattt taacttcata ctggactaca
      901 caaaggtacc atatacggta aatggccctt tcagcatcag cgaaaatgga gtagtgtttg
      961 agaacgcgat tgttactgac agatttggtc atgaaggaac ccttacagga ggattgcaat
     1021 atgaccattt caaagacatg cgtcttaacg ccagactgaa tgccgagcat ctacacggac
     1081 tcaataccac ggctgcagac aacgattcat actacggaag agcatttgcc accggtacag
     1141 tccgtttaag cggaccattc agaaaactcc tgctggatat gaatatcacc accgggcgga
     1201 actctaccat acatatcccg ctaggttcct catcaagcag ccatacctca ttgctcacct
     1261 ttgtggacaa cactactgtc aagtccatct cagcctatga ctcgctacag gtgatgaaca
     1321 ccatagctga aaagagaggc ggttacgaat ttgctgtaag aatgcgtgtc aatgccactc
     1381 ctgatgccga agtccatctc gaaatcaata aagccctggg cgatgtcctc aagactcgcg
     1441 gatccggaca gttggttatt aatgccgcca cagatttgtt tgacataaaa gggaactaca
     1501 atatcgatga aggcagctac aagttcgtga tgttgggact cgcttcccgt gacttcatta
     1561 tcaatccggg tggcactatc aatttcaccg gagatgtaat gaaatctgac ctcaaccttg
     1621 aggctatata ccgtacaaaa gcctccatag gcactctgat agccgatacc acctctataa
     1681 gtaccaggcg cacagtggac tgtggcatag gcatcaccgg aaaactcgcc aatcccgaac
     1741 tcaatttctc cattgatatt cccgatctgg accccaccat caaagggcgt gtggaaagtg
     1801 cactgagtac cgaagataaa cgtctcaaac agttacttac tctgctcctc tcaggcagtt
     1861 tcgttcccga cgaacagtca ggcattgtca acaacaccac cgtgctttac tccaatgcct
     1921 ccgagattat ggccaaccag ctcaataatg tattccgcca actcaacatt cctctcgacc
     1981 ttggattcaa ttaccaaccc ggagaaaagg gacgcgacat atttgacgta gcagtctcga
     2041 ctcagttgtt caacaatcgt gtcaatataa acggaaatat aggaaacagg cagtatatga
     2101 cctccaacca gagtgacctg gtgggtgatg ttgatgtgga aatcaaaatc gatcgtaccg
     2161 gcagggtacg cctcactcta ttttctcatt cggcagacca gtacagtaat tacctcgacc
     2221 agttacagcg caacggaata ggtttatctt atcaggaaga atttgatgac ttcagagaac
     2281 ttttcagaaa gatcttctgg agtaaaagac gccgcgatga gtacgccgct cagcaagcga
     2341 gagtgctaga gatacgtcaa aaagaccccc tgcaacgaat acaggaacaa aaatcaaagc
     2401 agacgcaaga aaccaccatc aggtgacacc ttaagtactg cttctcttcc caacacaagc
     2461 gaataattgg gctgctcgtg accgcacgga aatccgaaaa gcaccggaca atcgagacga
     2521 tcggtatgtt cctttatgag ggtatataca tctttttccc acttatcgct atccgccgta
     2581 tcggtaaact ggccgataat cacacctttg agacgatcca gcatacctgc acaacgcata
     2641 gtgagcaaca ttctatctac cgtgtgcatc ttctcgtcaa tatcctcaat aaacagaatg
     2701 gagt
//
LOCUS       DAOSIE010000205         2697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_32786_length_2697_cov_3.532551, whole genome shotgun sequence.
ACCESSION   DAOSIE010000205 DAOSIE010000000
VERSION     DAOSIE010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32786_length_2697_cov_3.532551"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1321)
                     /gene="ablA"
                     /locus_tag="PLI69_05930"
     CDS             complement(71..1321)
                     /gene="ablA"
                     /locus_tag="PLI69_05930"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOG42058.1"
                     /translation="MNLSRRKELFPEVADAQWDDWKWQVKNRIETLDQLKQYVKLTPE
                     EEEGVKKTLSTLRMAITPYYLSLINPDDSNDPVRKQAIPTGAETHQSAADLLDPLHED
                     EDSPTPGLTHRYPDRVLLLITDMCSMYCRHCTRRRFAGQKDAESPSERIDKAIDYIAR
                     TPQVRDVLLSGGDALMVSDAKLENIIKRLRAIPHVEIIRIGSRVPVVCPQRITDDLVN
                     MLKKYHPIWLNTHFNHPNEVTEESAKACAKLADAGVPLGNQSVLLRGINDCVHVMKKL
                     VHELVKIRVRPYYIYQCDLSMGLEHFRTPVSKGIEIMEGLRGHTSGYAVPTFVVDAPG
                     GGGKTPVMPQYVISQAPGKVVLRNFEGVITTYTEPDEYHFECNCEECRKESEKPFDGV
                     VALLEGQQMAIEPAFLQRKERSKH"
     gene            complement(1332..2372)
                     /locus_tag="PLI69_05935"
     CDS             complement(1332..2372)
                     /locus_tag="PLI69_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOG42059.1"
                     /translation="MKKGNKYGTHRVIEPKGVLPQPANKIDNNMDEIYDNEILIDVQT
                     LNIDSASFTDIANFCNHDKEEMKKEIFLNVELQGKHRNRRTGSGGMLLGRVEKIGDAL
                     IGKTDLKVGDKIATLVSLSLTPLRIDEILEIREDVDQVDIKGKAILFESGIYAKIPTD
                     MPEKLALSALDVAGAPAQTAKLCRPGDTVLIIGAGGKSGMLCCYEAKKRVGVTGKVIG
                     MTHSQRSTKRMEDLGFCDVVFAGNANDPIAMYEKISELTGGKMVDVTINNVNVPDTEM
                     TSILCTKNDGIIYFFSMATSFTKAALGAEGVGSDVTMIIGNGYTKGHAEITLQLLRES
                     EKLRNIFTELYA"
     gene            complement(2416..>2697)
                     /locus_tag="PLI69_05940"
     CDS             complement(2416..>2697)
                     /locus_tag="PLI69_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOG42060.1"
                     /translation="IPNLCWLVNAIPAGSTWCATGIGRHNFTLVAPAISMGGNVRVGF
                     EDSLYLSRGVLAKSNGELVAKVVRLAKELGREVATPAEARQILGLAPRQ"
BASE COUNT          707 a          638 c          590 g          762 t
ORIGIN      
        1 tgtattcttc tcaagcccga caatagacac gctgcggtat ttgagtatct catcaacaaa
       61 gggcataatc ctagtgctta ctacgctcct tacgctgtag aaatgccggt tcaatggcca
      121 tttgctggcc ttccaataga gcaacaaccc catcgaatgg tttctcagac tctttgcggc
      181 actcctcaca attacactca aaatgatatt cgtcaggctc ggtataagta gtaatcacac
      241 cttcgaagtt gcgcagtacc actttgccgg gggcttgaga aatcacatat tgaggcatta
      301 caggggtctt accaccacca cccggagcat ctaccacgaa ggtaggcacg gcataacctg
      361 aggtatggcc tctaagacct tccataatct cgataccctt tgaaacaggg gtacggaaat
      421 gttcaagacc cattgagagg tcgcactgat agatataata agggcgtacg cgaatcttga
      481 caagctcatg aaccaacttc ttcatcacgt gaacacagtc attaataccg cgcagcagaa
      541 ctgactggtt gccaagagga acacctgcat cggcaagctt tgcacaagcc tttgcggact
      601 cttcagttac ctcatttgga tggttgaagt gtgtgttaag ccatatggga tgatactttt
      661 taagcatatt gaccagatca tcagtaatac gctgagggca aactaccggt actcttgagc
      721 cgatacggat aatctctaca tgcggtattg cacggagcct ctttattata ttttcaagtt
      781 tggcatcgct aaccatgaga gcatcacctc cggaaaggag tacgtccctc acctgtggag
      841 tacgggcgat ataatctata gccttatcaa tcctctcaga tggactctca gcatctttct
      901 gaccggcaaa acgtcttctg gtacagtgac gacaatacat tgagcacatg tcggtgatca
      961 gaagcagtac tctgtccgga tatctgtgag taagacctgg agttggggaa tcctcatcct
     1021 catgtagagg gtcgagaaga tctgctgctg actgatgtgt ctccgcaccg gtagggattg
     1081 cctgtttgcg aacgggatca ttggagtcat caggatttat aagggaaagg taataagggg
     1141 taattgccat cctgagggta gagagtgtct ttttgacacc ctcttcctcc tcgggagtaa
     1201 gtttaacgta ttgtttgagt tggtcaagag tctcgatcct gttttttacc tgccatttcc
     1261 agtcatccca ctgggcatcg gcaacctcag gaaaaagttc ttttcttctt gataaattca
     1321 tatcctcaaa tttatgcgta aagttctgta aatatatttc tcagtttttc gctctctctg
     1381 agtaattgga gagtgatttc agcgtgacct ttggtgtaac cgttacctat gatcatggta
     1441 acgtcgctac ccacaccttc agcacctaga gcagcttttg taaagctcgt tgccatagag
     1501 aagaaataga tgatgccatc attcttggta caaagaatgg aggtcatctc agtatcggga
     1561 acgtttacgt tgtttatggt cacatcaacc atctttccgc cggtaagctc ggaaatcttc
     1621 tcatacattg cgataggatc gtttgcatta cctgcaaata caacatcaca gaaaccgagg
     1681 tcttccatgc gttttgttga cctttggcta tgagtcatgc cgataacctt acccgttacg
     1741 cctacacgtt tctttgcctc gtagcagcaa agcatacctg attttccacc tgctccaata
     1801 atgagaacag tatcaccggg cctgcaaagc tttgcagttt gtgcaggagc acctgcaacg
     1861 tcaagagccg aaagagccag tttttcaggc atatccgtag gtatctttgc gtagattcca
     1921 ctctcgaaaa gaattgcttt acccttgatg tccacctgat ctacatcttc acggatttca
     1981 agaatctcgt caatccgaag gggggtcaaa gagagagata caagagtagc aatcttatca
     2041 cctaccttga ggtcggtctt gccaataagt gcatcaccga ttttttcaac tctgccaagc
     2101 agcatgcctc cggaacctgt acgacgattg cgatgtttgc cctgtagttc aacattgaga
     2161 aatatctcct tcttcatctc ttccttgtcg tgattacaga aattggcaat atcggtaaat
     2221 gaagccgagt cgatattgag agtctgaacg tcaatgagga tctcattgtc atagatctcg
     2281 tccatgttat tgtcaatctt atttgccggc tgaggtagaa cgcccttagg ctcaattacg
     2341 cggtgcgttc catatttgtt accttttttc atcgttgtgt tattatgatt aaattgaatt
     2401 attgtttata aatgattact gtcttggagc caacccaagg atttggcgtg cttcggcggg
     2461 ggttgcaacc tcgcgaccaa gttctttggc caatcttaca acctttgcaa caagttctcc
     2521 attggatttg gcaagaactc ccctactgag ataaagacta tcttcaaatc caaccctcac
     2581 attaccgccc atggagattg cgggggcaac aagagtaaaa ttgtgacgac caatacctgt
     2641 agcacaccag gtgctgcctg caggaatggc attcaccagc caacagagat tggggat
//
LOCUS       DAOSIE010000206         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_33437_length_2658_cov_3.510181, whole genome shotgun sequence.
ACCESSION   DAOSIE010000206 DAOSIE010000000
VERSION     DAOSIE010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33437_length_2658_cov_3.510181"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1367
                     /locus_tag="PLI69_05945"
     CDS             216..1367
                     /locus_tag="PLI69_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOG42061.1"
                     /translation="MRRSAIRDLLSVANKPEIISFGGGFPSPDSFPIAELRQIMMDVM
                     DEQGAAALQYGSTEGYVPLREEIAKLYKEEGLDIPIKNILITTASQQAIDLLSRIFID
                     PDDIIICGLPSYLGALQAFWAYQCKPYGLRQDDRLEEAVNALVAAGKSPKFIYSIPDF
                     QNPSGVTMTVEERKNVIAVAEKYDLLIIEDSPYKQIRFEGESLPSMYSMCPDRVVLLG
                     TFSKIFVPGFRLGWVVAADSIIDRLIVAKQSADLCTPIFNQMVAARYLKSGKFHKNIV
                     KIKELYKHKKNVMIKAFEDHMPEGVTWTNPEGGLFLFLNLPPQYDTRELFEIAIKENV
                     AFVIGEAFHCDGSGKNTMRMNFSYASDEKIVEGIKRLSKAIRELYDRHS"
     gene            1459..2520
                     /gene="holA"
                     /locus_tag="PLI69_05950"
     CDS             1459..2520
                     /gene="holA"
                     /locus_tag="PLI69_05950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOG42062.1"
                     /translation="MAKQSTDTVQQFNEISKEIISGDIKPFYLLFGKEHYYIDKLCDL
                     LMDKVLPPEEKDFGQLVYYGSDVSANQVVNTARQFPMMISRQLVVVKEAQLMKKVEDI
                     DVYFDGIQPTTVLVICYKTPNDPAKSSKNIDKRTSFYKRAQKFGTVFESNQIPDYKID
                     RWVEEYARSQGLSIEPMAAKLMVEFAGTDISKLVLELDKLIKLLPEEHKSVTVATVEE
                     NVGISRDYSVFELTKALSIKDMRKAFRIVRFFSESGNRYPIQMILAALSSHFIRILRY
                     HALLQEGTPRNTILSQLGIHPYFGSEYDTAIRHYPVKKTMRVIALLKEYDQKSKSNSR
                     GNATDGELLQELVLRIADS"
     gene            complement(2517..2657)
                     /locus_tag="PLI69_05955"
     CDS             complement(2517..2657)
                     /locus_tag="PLI69_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HOG42063.1"
                     /translation="MEAKVGDTVTIFGEKPRALDLAEILDTIPYEIFTSVAKRVKRVI
                     ID"
BASE COUNT          768 a          550 c          588 g          752 t
ORIGIN      
        1 gttttaattg ttaaacttta taataacccg tccggagttt taccggacac gtatggtgaa
       61 atattaaaat gaaagaaata tcacaaagtt gcttattgtt gataataaaa atttgtacat
      121 tactttcgat ttgttatctt tgcgtagact tgtagaacta ataaagtaat agtaacatga
      181 atattgatca tttattgtcg gcgaacgcaa agagtatgag gcgttcagcc atcagagacc
      241 tcttgagtgt ggcaaacaag ccagaaatca tctcatttgg aggcggtttt cctagccctg
      301 actctttccc tattgcagag ttaagacaga ttatgatgga tgttatggat gagcagggag
      361 ctgcggctct tcagtatggt tcgacagagg gatacgttcc tcttcgcgaa gagattgcta
      421 aactctataa agaagaggga ctggacatac ccatcaagaa tattcttatt actactgctt
      481 cacagcaggc gatagatctt ctctctcgca tcttcataga ccccgatgat atcattatct
      541 gcggtttgcc ctcatacctt ggagcattgc aggctttctg ggcctatcag tgcaagccct
      601 atggcctccg ccaggatgac cgcctcgaag aggctgtcaa tgcactcgta gcagccggaa
      661 agagtccgaa attcatctat tcaattcccg atttccagaa tccttcgggt gtgaccatga
      721 cagttgagga gagaaaaaat gtgattgcag ttgccgagaa atatgacctt ctcataattg
      781 aggatagccc ttacaagcag attcgttttg aaggtgagtc actgccttcc atgtactcca
      841 tgtgtccgga tagagttgtc cttctaggga cattttccaa gatattcgta cccggattcc
      901 gtctcggatg ggtagttgcg gctgatagca tcatagatag gcttatcgta gcaaaacagt
      961 ctgccgacct atgtacgccg atattcaatc agatggtggc agcccgctat cttaaatccg
     1021 gaaagttcca taagaacatt gtaaagatca aggagctgta caagcacaag aaaaatgtaa
     1081 tgataaaggc ttttgaagat catatgccgg agggtgttac ctggaccaat cccgaaggag
     1141 gccttttcct attcctcaac ctcccgccgc agtatgacac tcgtgaactc ttcgaaatcg
     1201 ccataaagga gaatgtggca tttgttatag gagaggcatt ccactgtgac ggcagtggta
     1261 agaatacaat gcgtatgaat ttttcatatg cctctgacga gaaaatagtc gaaggcatta
     1321 agcgcctttc aaaagctata agagaacttt acgacagaca ttcatagatt tctatgatta
     1381 catagtttgt gtgtttggtg gggccggcta aagagtcggc ccctttttta ttgccaaaaa
     1441 aagttacctt tgtaaattat ggcaaaacag agtacagata ccgttcaaca atttaatgag
     1501 atctcaaaag agattatctc gggagatatt aagccatttt acctgctttt tggcaaagaa
     1561 cattactata tagacaagtt atgcgacctt ttgatggata aagttctgcc tcctgaggag
     1621 aaggatttcg gtcagttggt ctattacggt tccgatgtct ctgccaacca ggtggtaaat
     1681 acagcccgtc aattcccgat gatgatttcc cgtcagctgg ttgtggtcaa agaggcacag
     1741 ttgatgaaaa aggtagagga tattgatgtc tattttgacg gtatccagcc cacaaccgtt
     1801 cttgtgattt gttataagac tcccaacgac cctgcaaaat cttcaaagaa tatagacaag
     1861 cgtacgtctt tttacaaacg ggctcaaaaa ttcggtaccg tattcgaatc caaccagatt
     1921 cctgattata agattgacag atgggtggag gaatatgccc gctctcaagg cctctccatt
     1981 gaacccatgg cggcaaagtt gatggttgaa tttgccggaa ccgacatcag caaactggtt
     2041 ctcgagcttg ataaactcat taaactgctt ccggaagagc ataaatccgt cacagtagcc
     2101 actgtagaag aaaatgttgg aataagtcgc gattatagcg tatttgagtt gactaaagcc
     2161 ctttcgataa aggatatgcg gaaggccttt agaatagtcc ggtttttctc agaatcgggg
     2221 aacagatatc ctatacagat gattttagca gcactctcat ctcattttat caggattcta
     2281 aggtaccatg cgctacttca ggagggaact cccagaaata caatactctc tcaacttggg
     2341 attcatccct attttggcag tgagtatgat actgccatcc gccactatcc tgtcaagaag
     2401 acgatgaggg tgatagctct gcttaaagag tatgaccaaa aaagtaaaag caactctcgc
     2461 ggaaatgcta ccgatggaga gttgcttcag gaacttgttc tcaggatagc ggactcttag
     2521 tcaatgatca ctctcttcac tcttttggca actgaagtga agatttcgta aggtattgtg
     2581 tcaagaattt ctgcaaggtc gagtgctctc ggcttttcac caaatattgt gacggtatca
     2641 ccaacttttg cctcaatt
//
LOCUS       DAOSIE010000207         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_33756_length_2639_cov_3.441176, whole genome shotgun sequence.
ACCESSION   DAOSIE010000207 DAOSIE010000000
VERSION     DAOSIE010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33756_length_2639_cov_3.441176"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLI69_05960"
     CDS             <1..192
                     /locus_tag="PLI69_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG42064.1"
                     /translation="SLQRAKAVADYLKSLGIEASRIVFEGKSFREPIADNSTAEGRRL
                     NRRVEVFVYANDIMIRNSR"
     gene            205..912
                     /locus_tag="PLI69_05965"
     CDS             205..912
                     /locus_tag="PLI69_05965"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOG42065.1"
                     /translation="MRPFYLGLLLALSLFLSSCSRINTGRTDGTYFYPLEPFTGVKVG
                     DDVFTRIYVQPGEPGIEIICDENIRNYLYGKILNGVLHFGVKSGAIPGASVDMEVRIY
                     TETLSYLEVGDNTPVGIYGDVSGDLEIKLTGNSNVRCNSMDLNNLTINATGGGAVLKG
                     KADKAVINCYSGASIMDFNFSIEDLIIYMSGASRAELTVNNTINADLRGGSTFTYRGE
                     AYVQTKKLSGGSVLNKD"
     gene            1022..1672
                     /locus_tag="PLI69_05970"
     CDS             1022..1672
                     /locus_tag="PLI69_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOG42066.1"
                     /translation="MKKTISLKDAVAMVKDGATIMVGGFLGTGAPAQLVDALVESGVK
                     DLTVIANDTVFDGKGWGKLIENRQVKKIISSYIGGNAASIDQMNAGETEVELVPQGTL
                     AERIRAKGAGLGGVLTPTGLDTVVEKGKKRITIDGKDYLLEKPLGADFALIGGSVVDE
                     SGNVFYRGTTRNFNPTMAQAADVVIVEAEKIVPVGELAPEVIHTPFIFVDYIYVKQ"
     gene            1705..2091
                     /locus_tag="PLI69_05975"
     CDS             1705..2091
                     /locus_tag="PLI69_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminobutyryl-CoA ammonia lyase"
                     /protein_id="HOG42067.1"
                     /translation="MTNKAMIRVRMSSQDAHYGGNLVDGAKMLQLFGDVATELLVKRD
                     GDEGLFKAYDMVEFFAPVYAGEFIEAVGEIVSEGNSSRKMVFEARKVIVPRHDISDSA
                     ADFLEEPIVVCRASGTCVTPKSCQRK"
     gene            2094..>2639
                     /locus_tag="PLI69_05980"
     CDS             2094..>2639
                     /locus_tag="PLI69_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOG42068.1"
                     /translation="MDKLIITAAICGAEVTKEHNPAVPYTVDEIVREAKSAVDAGAAI
                     VHLHVREDDGTPTQSKARFKECIDAIYKVCPDVILIPSTGGAVGMTAEERLQPTELFP
                     EMATLDCGTCNFGDEVFENTIPMMRDFGKRMLENNIKPEYECFEMGHLDTILTMARKG
                     EVPGHPMHFNFVLGVPGCTSAT"
BASE COUNT          736 a          506 c          670 g          727 t
ORIGIN      
        1 tctctacaac gtgccaaagc tgttgcggat tatcttaaaa gcctaggtat agaggcaagt
       61 aggattgtgt ttgagggcaa atcttttaga gaacccatag cagataactc taccgctgaa
      121 ggtcgcaggc tcaatcgccg tgtggaggta ttcgtttatg caaatgatat aatgatccga
      181 aattcaaggt agtagaagaa atagatgaga cctttttatc tgggactcct attagcgcta
      241 tcgcttttcc tatcttcctg ttcgagaatc aatacgggca ggaccgatgg gacatatttc
      301 tatcctttgg aaccctttac aggggtcaaa gtcggagatg atgtttttac caggatttac
      361 gtgcagccag gggagcccgg catagagata atctgtgatg aaaatatacg gaattatctc
      421 tatggcaaga tactgaatgg agttttgcat tttggtgtga aaagtggtgc tattccaggg
      481 gcaagtgtag atatggaggt gaggatatat actgagactc tgagttacct tgaagtcggt
      541 gacaataccc ctgtcggtat ctatggggat gtttccggtg accttgaaat taaattgacc
      601 ggcaatagca atgtaagatg caattccatg gatttaaata atctgactat caatgctacc
      661 ggaggtgggg cagtcttgaa gggcaaggct gacaaagcgg ttataaactg ctattcaggg
      721 gcttcaatta tggatttcaa tttttcgata gaagatctca tcatctatat gtccggtgcg
      781 agcagagccg agttgacggt aaataataca attaatgcag atctgagggg aggaagtact
      841 ttcacttaca ggggcgaagc ttatgtacaa acgaaaaagc tcagcggagg ctctgtgctg
      901 aataaagatt aaaataatct tggaaatcgt gatgaatttg attaattttg cggattgcgt
      961 taaacatagt ttaacaaatc cgcaaaattg cttttagctg aacaattaaa ttatctatga
     1021 tatgaaaaaa actatttctc ttaaggatgc cgttgcaatg gtaaaagacg gcgctacaat
     1081 tatggttgga ggcttcctcg gtactggagc tcctgcgcag ttggttgatg ctcttgtgga
     1141 gagcggagtc aaggatctta cagtgattgc caacgacacg gtatttgatg gaaagggctg
     1201 gggcaaattg attgagaacc gccaggttaa gaagattata tcctcctata tcggaggtaa
     1261 tgctgcatcc attgatcaga tgaacgcggg tgaaacggag gtcgaactgg taccgcaggg
     1321 tactcttgcg gagcgtattc gtgccaaggg tgcaggtctt ggcggagtgt tgacacccac
     1381 cggcctggat accgtagttg aaaaaggtaa gaagagaatt accattgatg gaaaggacta
     1441 cctgttggag aagcctcttg gagctgattt tgcgttaatt ggcggttcgg tggtagatga
     1501 atcgggtaat gtattttata ggggaactac gcgcaatttc aatcccacaa tggcacaggc
     1561 tgccgatgtt gtaattgtgg aagcggaaaa aatagttcct gtaggcgaac tggcccctga
     1621 ggttatccac acccctttta tttttgtaga ctacatctat gtaaaacaat aatcagacaa
     1681 taacagattt taaagaaact atttatgaca aacaaagcaa tgatcagagt acgtatgagc
     1741 tcacaagatg ctcattacgg cggtaatttg gttgatggcg caaaaatgct tcagcttttc
     1801 ggtgatgtcg ctaccgaact tttggttaaa cgcgacggtg atgagggtct cttcaaggcc
     1861 tatgatatgg tagaattctt tgctcccgtc tatgccggtg agtttattga agctgtagga
     1921 gagattgttt ccgaaggtaa ttcttccagg aaaatggtat ttgaggcccg caaggtaatt
     1981 gttccccgtc acgatatcag cgactcagct gcagactttc tcgaagagcc catcgttgtt
     2041 tgccgtgcaa gcggtacatg tgtaacccct aaatcttgtc agagaaaata attatggata
     2101 aacttatcat tactgccgct atctgcggcg ccgaggttac taaggagcat aatcctgcgg
     2161 ttccctatac tgttgatgaa attgtacgtg aggctaagtc tgcggttgat gccggagcag
     2221 ctatcgtgca tcttcatgtc cgtgaggatg atggcacgcc tactcagagc aaggcacgct
     2281 ttaaagagtg tattgatgct atctataaag tctgccctga tgttattctt attccttcca
     2341 ccggaggcgc agtaggtatg acagctgaag agagacttca gccgactgaa cttttccctg
     2401 aaatggcaac cctcgattgc ggaacctgta attttggtga cgaagtattt gaaaacacaa
     2461 tacccatgat gcgtgatttc ggcaaaagga tgctggaaaa caacattaag cctgagtacg
     2521 agtgctttga aatgggacac ctggatacga tacttaccat ggctcgcaaa ggcgaggtcc
     2581 ccggtcatcc catgcacttc aactttgtcc tcggcgtacc cggttgcacg tctgcaacc
//
LOCUS       DAOSIE010000208         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_34293_length_2608_cov_4.235018, whole genome shotgun sequence.
ACCESSION   DAOSIE010000208 DAOSIE010000000
VERSION     DAOSIE010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34293_length_2608_cov_4.235018"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLI69_05985"
     CDS             <1..199
                     /locus_tag="PLI69_05985"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOG42069.1"
                     /translation="YSPSDVKSLLESYPEFQQGSLLFATYGSTTAKAMKDAKLKTEIE
                     APTPEAPSIAKALLNYFEKLH"
     gene            200..601
                     /gene="rnpA"
                     /locus_tag="PLI69_05990"
     CDS             200..601
                     /gene="rnpA"
                     /locus_tag="PLI69_05990"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOG42070.1"
                     /translation="MDFGFPKRERVCLKKDIDALLESGEVVFRYPLRVLYLRNTGSES
                     HRALFSVPKKSFKRAVARNLLKRRMREAYRHLKHLLGGEPFYDLMFIYVAKDIKDYGS
                     IFSKMGEILDKIADGGTQSSDLSTTDAAEVL"
     gene            498..773
                     /gene="yidD"
                     /locus_tag="PLI69_05995"
     CDS             498..773
                     /gene="yidD"
                     /locus_tag="PLI69_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOG42071.1"
                     /translation="MAPSSQKWEKFWTKSLTVARKVVIFPLLMLLKFYKFFISPYVGN
                     ACRFTPTCSVYAMEALSKHGLFKGTYLAIRRILRCHPWGGSGYDPVP"
     gene            complement(792..>2608)
                     /locus_tag="PLI69_06000"
     CDS             complement(792..>2608)
                     /locus_tag="PLI69_06000"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG42072.1"
                     /translation="VINKSDIMKVIKMKGPIGWIVSTLAMSALGFNKVNRSFYRCKHL
                     TGPDFSEAILKDIGISIDVNSAQLDYLPLEGPFITISNHHFGGADGLMLSSIVGAIRP
                     DLKILTNFILSMIPTLEDSFLPVNPFTDATSKRKSISGIRMAMEHIEKGGCLGLFPAG
                     EVATIQKGENRTSLKRGRVVEDIPWPTNMVKLIKNANVPVVPIYFEGENSKWFHFLGR
                     IHPMLRTLALANQLFNKRGKTIPMRIGWPVFPSEIADFDNLDNLGRHLRSRVYALQAD
                     FADPNKENKMKHVSPIALPKDRKAILKELLRIKDKKLYQTAQYHCFLADYEDIPNLIH
                     EIGRRREEAFRASGEGTNTALDIDSFDQYFKHLILWDASQKKIAGAYRLGIGEEIFAK
                     HGGIGGFYSSSLFDYNDEFEPTLRKAIELGRSFVSVEYQKEALPLMLLIKGLMHSLML
                     YPNTEYFIGPVSISNSYPRFFQSLMFYYIMEKCSTDFGRDLVSPLTPFVPDYLKTNPE
                     YLLMGKMGTLEKFDKYLMRLSDNKYRIPTLVKKYIKINSRIICFNVDPLFNYSLDGLV
                     LLKITDFPKEELLMMTRDIENPFEREKIFNRFGYSLKE"
BASE COUNT          678 a          556 c          627 g          747 t
ORIGIN      
        1 atacagccct tcagatgtaa agtctcttct cgaaagttac cccgaatttc aacagggttc
       61 actgctcttc gccacctacg gctctactac tgccaaagct atgaaggatg caaagctcaa
      121 gactgagatt gaggcaccca ctcccgaggc tccttcaatt gcaaaggctc tgctcaacta
      181 tttcgaaaag ttgcattaga tggatttcgg gtttccaaaa cgcgagaggg tctgcttgaa
      241 aaaagatatt gatgctcttt tggaaagcgg ggaggtcgtg ttcaggtacc ccttgagagt
      301 tttatatttg agaaacaccg gatcggaatc tcatcgggcg ctcttttccg tacctaaaaa
      361 atcattcaag cgtgccgtag cacgcaatct cctcaaaagg aggatgcgtg aggcttacag
      421 acatttgaaa caccttttgg gaggggagcc tttttacgat ttgatgttta tttatgtggc
      481 aaaagatata aaagattatg gctccatctt ctcaaaaatg ggagaaattc tggacaaaat
      541 cgctgacggt ggcacgcaaa gtagtgatct ttccactact gatgctgctg aagttttata
      601 agtttttcat ctctccgtat gtcggcaatg cttgcaggtt cactccaacc tgctcggtct
      661 atgccatgga agccctaagt aaacatggat tatttaaagg gacgtacctt gcgataagac
      721 gtattctgcg ctgccatccc tggggagggt caggttatga tcccgtgcca tgactaaagg
      781 gggtcgatct cttactcttt gagtgaatat ccgaaacgat tgaatatctt ttcgcgctca
      841 aatggattct cgatgtccct tgtcatcatc aaaagttctt ctttggggaa atcagtaatt
      901 ttgagtagca caagcccatc aaggctataa ttgaagagcg ggtcaacatt gaaacatatg
      961 atccgcgagt tgatttttat atattttttg acaagggtgg gaatacgata tttattgtcg
     1021 gaaaggcgca taagatattt gtcaaatttc tcaagcgtac ccattttacc catcagaaga
     1081 tattcggggt tggtcttgag ataatcgggt acaaaaggtg ttagcggtga taccaggtca
     1141 cgtccgaaat cagtactaca tttttccatt atataataaa acatcagact ctggaaaaat
     1201 ctggggtagg agttggaaat actcacgggg ccaatgaagt attcggtatt gggatagagc
     1261 atcaaagagt gcataagtcc cttgatcagc agcatcagag gaagggcctc cttttggtat
     1321 tcaacgctta caaagcttct gcctaactct atggccttgc gtagggtggg ttcgaattca
     1381 tcattataat cgaataggct ggatgagtag aatccgccaa taccaccgtg ctttgcaaaa
     1441 atctcttctc cgataccgag acgatacgca cctgctatct ttttctggga ggcgtcccaa
     1501 aggatcaggt gtttgaagta ttggtcaaaa gagtcaatat caagagctgt attggtgccc
     1561 tctccgctgg ccctgaaggc ttcttcgcgt ctgcgcccta tttcgtggat gagattgggg
     1621 atatcttcgt agtctgccag gaagcagtga tattgggccg tctgatagag ttttttatcc
     1681 ttaatcctta acaactcctt gagtatcgcc ttgcgatctt ttggaagagc aatcggagag
     1741 acatgcttca ttttattttc cttgttcgga tcggcaaaat cggcctgtaa cgcatagact
     1801 ctactgcgga ggtggcgtcc aaggttgtca agattgtcaa aatcggcgat ttccgaaggg
     1861 aaaacaggcc aacctatgcg catgggtatg gtctttccac gcttgttaaa gagttgattg
     1921 gccaaagcca atgttcgcag catggggtga attcttccca aaaagtggaa ccacttgcta
     1981 ttttctcctt caaagtagat tggaactacc ggtacatttg cattttttat gagcttgacc
     2041 atgttggttg gccaggggat atcttccaca accctacctc ttttgagcga agtgcggttc
     2101 tctcctttct gtatggtggc tacttcacct gcggggaaaa gaccgaggca gccccccttc
     2161 tcgatatgtt ccattgccat tcttatgccg gaaatgcttt tgcgcttgct ggtggcatcg
     2221 gtaaaaggat tgaccggcag aaaactatcc tcaagagtgg gaatcatgga gaggataaag
     2281 ttggtgagga tcttcaggtc gggacggatt gcacctacaa ttgagctgag catcagtccg
     2341 tcggctccgc caaaatggtg gtttgaaata gtgatgaatg gcccttcaag gggcaggtaa
     2401 tccagctgtg cagaattgac atctatggag atgcctatat ctttaagtat cgcttcggaa
     2461 aaatcggggc cggtgaggtg tttgcagcga taaaagctgc ggttaacctt gttgaatccc
     2521 agagctgaca tagccagcgt ggatactatc cagccaatcg gacccttcat ctttatgact
     2581 ttcattatgt ccgatttatt gattactt
//
LOCUS       DAOSIE010000209         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_34316_length_2607_cov_4.019592, whole genome shotgun sequence.
ACCESSION   DAOSIE010000209 DAOSIE010000000
VERSION     DAOSIE010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34316_length_2607_cov_4.019592"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLI69_06005"
     CDS             <1..539
                     /locus_tag="PLI69_06005"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG42073.1"
                     /translation="QYSGKTVAECCEISGMEVWPMIRHLLISEKLNVQMAGFAMKEEN
                     IRMEYRHPLSMPASDGSVYSPDGPLGEKMPHPRSYGTFPRFFQKYVREDKILDLATAV
                     YKCSALPASRLKLRERGLLIPGYWADIVVFNPDTIEEKSTYDNPHQFPVGIEHVFVNG
                     VWTIKDGAHTGKLAGMIV"
     gene            560..1357
                     /locus_tag="PLI69_06010"
     CDS             560..1357
                     /locus_tag="PLI69_06010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG42074.1"
                     /translation="MKSVRTIIPALCALIIISCSGNIPKENVFEYDEFGVVTKINPDE
                     KSVWLVFTAHYSLDDNGYFENFDGVVPVLNTLKEKEVKGSFFPTGICLEVEKYQEAVR
                     RIIDEGHYLSSHSFNHLLLSEDGRTLVSADSIKADFALMEASLEKYGLKKEQYNRLIP
                     PYETYNQETADAMRDLGYKLVNPTPGFKTGMDWTSPGASNYISTEEILEGIWQYESEN
                     SMNGVILLIHAMNYPDRVDEDRPYTYLGEIIDTLRSKGYTFKTMFDL"
     gene            complement(1367..2392)
                     /locus_tag="PLI69_06015"
     CDS             complement(1367..2392)
                     /locus_tag="PLI69_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG42075.1"
                     /translation="MIEKTILLTDLNPIDFYGVNNRNFDLLTSFFPKIKVVARGAEVY
                     VTGPADEIRLFEEKLEKVMKICRLKGALTEYDMEQVCLERIISAETGKVDMPNDEDYI
                     IVYSNDGRTIKARTKNQKRLVKEYYKNDLLFALGPAGTGKTYTAIALAVRALKNREVK
                     RLILTRPAVEAGERLGFLPGDLKDKLDPYLQPLYDALGDMIPAKRLHSLMEEGTIQIA
                     PLAYMRGRTLDRAFVILDEAQNTSLGQLKMFLTRMGNDAKFIVTGDATQIDLPDKRNS
                     GLVRGIEIVKNIRGISIIEFDRDDIVRHPLVTKIVDAFEEHEKRESREKGELSELRKK
                     DQNNKTE"
     gene            complement(2385..>2607)
                     /locus_tag="PLI69_06020"
     CDS             complement(2385..>2607)
                     /locus_tag="PLI69_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42076.1"
                     /translation="STIALLPPKIDYWWCARTNKLERNYTVVPIMEYIDKKEQFKEGQ
                     YEKMVREWNAIVKKFGIDTTGKRITYIYD"
BASE COUNT          668 a          597 c          588 g          754 t
ORIGIN      
        1 ctcaatattc cggtaaaact gtggccgagt gctgtgaaat ctccggtatg gaggtttggc
       61 ctatgatccg acacttgctc atctctgaga aactcaatgt tcagatggcg ggatttgcga
      121 tgaaagagga gaatatccgg atggagtatc gccatccgct ttcgatgccc gcttccgatg
      181 gcagcgtcta ttctcccgat gggccactgg gcgaaaaaat gcctcaccct cgctcgtatg
      241 gcacttttcc ccgtttcttc caaaaatatg tgcgcgagga taagattttg gatttggcta
      301 ccgcagttta caagtgcagc gcactgcctg cttcccgtct taagctcagg gagcgcggat
      361 tgctgatccc cggctattgg gcagacatag tagtcttcaa tcccgatacc atcgaggaaa
      421 aaagcaccta cgataatcct caccagttcc ctgtgggaat agaacatgta tttgtgaatg
      481 gtgtctggac aattaaagat ggtgcgcata ccggcaagct cgcgggtatg attgtgtaat
      541 tatgaaattg aggaacatta tgaagtcagt tagaacaatt attcctgcgc tctgcgccct
      601 gataatcatt tcctgttcgg gaaatattcc gaaggagaat gtcttcgaat atgacgagtt
      661 tggggtcgtg accaagatca accccgatga aaaatctgtt tggttggtat tcaccgccca
      721 ttatagcctt gacgataatg ggtattttga gaatttcgac ggagtggtac ctgtgctcaa
      781 caccctgaaa gaaaaggagg tcaaaggatc ctttttcccg acaggtatct gcttagaggt
      841 ggaaaaatat caagaagctg taaggagaat catagatgaa ggtcactatc tttcctcaca
      901 ttccttcaat catctcctgc ttagcgaaga tggccgcact ctcgtgagtg ccgactccat
      961 taaagccgat tttgcactaa tggaggcctc tttggagaaa tatggtctga aaaaggagca
     1021 atataaccgg ctgatcccac cctatgagac ctacaatcag gagacggctg atgctatgcg
     1081 tgacctgggt tataaattgg tcaaccctac acccggattc aagacaggaa tggattggac
     1141 ctcaccggga gcttctaatt atatttcaac tgaggagatt ctggaaggaa tatggcaata
     1201 cgagtcggag aatagtatga atggcgtaat cctgctgatc cacgcaatga actatcccga
     1261 tcgagtagat gaagatcgtc cctatacata tttgggtgag atcatagata cccttcgatc
     1321 caaaggatac accttcaaga ccatgtttga cctttagcgt ttcgctctac tcagtcttgt
     1381 tgttttgatc ctttttgcgt aattcgctca attcgccttt ttcgcgtgat tcgcgttttt
     1441 cgtgttcctc gaaagcatcc acaattttgg ttacgagggg gtgacggaca atgtcatccc
     1501 ggtcaaattc gatgatgctg ataccgcgaa tattttttac aatttcgata cctcgtacaa
     1561 gcccgctgtt gcgcttatcg ggaagatcta tctgagtggc atctccggtc acgataaact
     1621 ttgcgtcatt tcccatccga gtgagaaaca tcttgagttg accaagcgaa gtattctgag
     1681 cctcatccaa aatcacgaag gctctgtcga gcgtacgtcc tctcatgtag gccaacggtg
     1741 caatctgtat agtaccctct tccataagtg aatggagtcg ctttgcggga atcatatctc
     1801 caagtgcatc ataaaggggc tggaggtaag ggtcaagttt atccttaagg tctccgggca
     1861 gaaatcccaa tctttcaccc gcctctacgg cagggcgggt aagtatcaga cgtttgactt
     1921 cacggttttt gagcgcacgg acggccaggg caatagccgt ataggtttta cctgtaccgg
     1981 caggtcctaa ggcaaacaag agatcattct tgtaatactc ttttaccagg cgtttctggt
     2041 ttttagtgcg tgcttttatg gtccggccgt cattactgta aacgatgatg taatcttcgt
     2101 cgttaggcat gtcaacctta ccggtctcag cagaaataat tctttccaaa cagacctgtt
     2161 ccatatcata ctcggtcagc gcacctttta gacggcatat cttcatcacc ttttccaatt
     2221 tctcctcaaa gagacgaatc tcgtcagcag gtcctgtcac atagacttcc gcacctctgg
     2281 cgacaacttt tatttttgga aagaaggagg tcaaaaggtc aaaatttcta ttgttgaccc
     2341 cataaaaatc tatcggattg agatctgtca gtaaaattgt tttttcaatc atatatgtat
     2401 gttattcttt tcccggtggt atctattccg aattttttta caatggcatt ccattcacga
     2461 accattttct catattgtcc ctccttaaat tgctcctttt tatctatata ctccataatt
     2521 gggactaccg tatagttgcg ctccaatttg ttggttcttg cgcaccacca gtaatcaatt
     2581 ttcggaggaa ggagagcaat ggtcgag
//
LOCUS       DAOSIE010000210         2605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_34354_length_2605_cov_6.181961, whole genome shotgun sequence.
ACCESSION   DAOSIE010000210 DAOSIE010000000
VERSION     DAOSIE010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34354_length_2605_cov_6.181961"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1211)
                     /gene="trpS"
                     /locus_tag="PLI69_06025"
     CDS             complement(72..1211)
                     /gene="trpS"
                     /locus_tag="PLI69_06025"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOG42077.1"
                     /translation="MGKIILTGDRPTGRLHIGHYVGSLRRRVELQNSGEYEKIFIMIA
                     DAQALTDNADNPEKIRQNIIEVALDYISAGLDPEKSVLFIQSQISELTELTFYYMNLV
                     TVQRLQRNPTVKQEIQVRGYSESAEDSHNKVGTPVGFFCYPISQAADITAFKANIVPV
                     GEDQVPMIEQAREIVRKFNSTYGETLVEPQALLPDNSACMRLPGTDGKSKMSKSLGNC
                     IYLSDSAEEVWEKVKGMYTDPLHIQVSDPGHVEGNTVFTYLDAFCKDEHFEKYGDSFH
                     GRKVSFDFHNLEEVKEQYRKGGLGDMMIKTFLNAVLNDVLEPMRQRRKELEQNIPYVY
                     EVLKKGSETARAAAAETLADVKRAMRINYFEDSELIAAQAEKYRV"
     gene            complement(1223..2260)
                     /gene="recA"
                     /locus_tag="PLI69_06030"
     CDS             complement(1223..2260)
                     /gene="recA"
                     /locus_tag="PLI69_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOG42078.1"
                     /translation="MAKGKEEPGQTTAGKNLEKLKALQATIDKIEKDFGKGTIMKMGD
                     QPSFEVSIIPTGSIALDNALGIGGYPRGRVIEIYGPEASGKTTLAIHAIAEAQKAGGI
                     AAIIDAEHAFDRTYAINLGVDIDTLLISQPDNGEQALEIADNLIRSGAIDIVVIDSVA
                     ALTPKAEIEGDMGDNKVGLQARLMSQALRKLTANIAKTNTCCIFINQLREKIGIMFGN
                     PETTTGGNALKFYASVRVDVRKITQLKDGEETTGNRTRARIVKNKLAPPFRKAEFDIV
                     FGEGISRVGEIVDLGVDFDIIKKSGSWFSYGDQRLAQGRDAVKRLLQDNPELCDEIEV
                     KIREKLKEVKD"
     gene            2391..>2605
                     /locus_tag="PLI69_06035"
     CDS             2391..>2605
                     /locus_tag="PLI69_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOG42079.1"
                     /translation="MKPKLLGMSLLQLEELVADLSLPKYVARQIADWLYAKGTREISG
                     MTNLSIAVRKALEERYQIGHTPYIEKF"
BASE COUNT          637 a          657 c          558 g          753 t
ORIGIN      
        1 ataagctaac tcggaactca ggacttccac tcctcataaa actcgcaccc cgcaaccctg
       61 aaactcgcac ctcagacacg atatttctcg gcctgagctg ctatcagttc gctgtcttcg
      121 aaataattga ttcgcattgc gcgtttgaca tctgcaagag tctctgctgc tgccgcacga
      181 gccgtttctg aacccttttt taggacttcg taaacataag gaatattctg ctccagctct
      241 ttgcggcgct gacgcatcgg ctctagaaca tcatttaata ctgcgtttag gaaagtcttg
      301 atcatcatat caccaagacc tcccttgcgg tactgctcct taacctcttc cagattgtgg
      361 aagtcaaaac ttactttgcg gccgtggaaa gagtcaccat acttttcgaa atgctcatcc
      421 ttgcagaagg catccaggta tgtaaacacg gtgttgcctt ctacatgacc cggatccgaa
      481 acctgaatat gcagggggtc cgtatacatc cccttaacct tttcccacac ctcttctgcg
      541 gaatcagaga gatatatgca gttgccaagt gatttgctca ttttggattt accatcggta
      601 cccggaagtc gcatacaagc ggaattatca ggcagaagcg cctgaggctc cacaagagtc
      661 tctccatagg tggagttgaa tttgcgcaca atctcacgtg cctgctctat catcggcacc
      721 tgatcttcac cgactggcac aatattggcc ttgaatgcag tgatatccgc tgcctgactt
      781 ataggatagc agaaaaatcc gaccggagta cctaccttgt tgtgagagtc ctcagcactt
      841 tcactatatc cacggacctg tatctcctgc ttcacggtag gattgcgctg gaggcgctgt
      901 accgtcacga gattcatata gtaaaatgtg agctcggtga gttcgcttat ctgtgactgt
      961 atgaagagga ccgacttttc ggggtcaagt cctgccgata tatagtcaag ggctacttct
     1021 atgatatttt ggcgaatttt ttcgggattg tcggcattat cagtaagggc ctgcgcatcc
     1081 gctatcatta taaatatctt ctcatattcg ccggaattct gaagctccac ccttctgcgg
     1141 agcgatccca cataatgtcc aatatggaga cgaccggtag ggcggtctcc tgtcaaaata
     1201 atctttccca tcaattagtc acttaatctt ttacttcctt caatttctcc ctaatcttga
     1261 cttcaatctc gtcacaaagt tccggattgt cctggagtag gcgtttgact gcatcacgtc
     1321 cctgcgctag acgctggtca ccataactga accacgatcc gctctttttt attatatcga
     1381 aatcgacgcc gaggtcaact atttcgccca cacgggaaat gccttctccg aatacgatgt
     1441 caaactctgc cttacggaag ggaggagcca atttgttttt aactatcctt gcgcgtgtac
     1501 gattacctgt agtctcttcg ccatctttga gctgcgtgat tttacgaacg tcaacacgaa
     1561 ccgatgcata gaacttgagt gcattaccgc ctgtagtagt ttccggattg ccaaacatga
     1621 ttccgatctt ttcgcgaagc tggttgatga aaatacagca ggtattggtc tttgcaatat
     1681 tagccgtcag tttacgtagt gcctgactca tcagacgggc ctggagacca accttattgt
     1741 cgcccatatc accttcaatt tcggccttgg gtgtcaaagc ggctaccgag tctatgacca
     1801 caatgtcaat agctccggaa cgtattagat tgtcggcaat ttcaagggcc tgctctccat
     1861 tgtcgggctg tgatatcaaa agagtatcga tgtctactcc gaggttgatc gcataggtgc
     1921 ggtcgaatgc atgttctgca tcaatgatgg ctgctatacc accggctttc tgggcctctg
     1981 caatggcgtg tatggccaga gtggtttttc cactcgcttc agggccataa atctcgatta
     2041 ctctgccacg aggatacccc ccaatgccga gggcattatc cagcgcaatg gagccggtag
     2101 gaataataga aacttcgaaa ctcggctgat ctcccatctt catgatcgtc ccctttccga
     2161 aatccttctc aatcttgtca atagtagcct gaagggcttt tagcttctcc aggttcttcc
     2221 ctgcagttgt ctgtccgggt tcttcttttc cttttgccat aatctgtttt tttgaatgtt
     2281 agtaaatagc tataataatc tgcttattcc tgctacaaat atactccatt tttatgtgaa
     2341 aacattcttt ttgtattaaa tttgcaaagg gaaaaagaaa tttgcgaaag atgaaaccta
     2401 agttactggg aatgtcgttg ttgcagctcg aagagctagt ggctgacctt tcgttgccca
     2461 aatatgtagc caggcagata gctgattggc tctacgcaaa aggcaccaga gagatatccg
     2521 gcatgaccaa tctttccatt gcagtccgta aggctctcga agagcgctat cagataggtc
     2581 acactcctta catagaaaag ttcat
//
LOCUS       DAOSIE010000211         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_34473_length_2598_cov_3.364530, whole genome shotgun sequence.
ACCESSION   DAOSIE010000211 DAOSIE010000000
VERSION     DAOSIE010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34473_length_2598_cov_3.364530"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..454
                     /locus_tag="PLI69_06040"
     CDS             110..454
                     /locus_tag="PLI69_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOG42080.1"
                     /translation="MKLKEIVALVEGKVICGEQNLDESVEYAFSSDLMSDVLTLRITD
                     FLLVTGLSNIQSIRTAEMSDLKYILIVRDKKVTQDMIELADDNDMTIITTEFSMFKTS
                     GLLYNAGLKPVY"
     gene            457..867
                     /locus_tag="PLI69_06045"
     CDS             457..867
                     /locus_tag="PLI69_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG42081.1"
                     /translation="MELVYTVEGGDFNKAGHASSQIKRVLKQLGVPPDIIKRVVVAMY
                     EAEVNIVAHAWRGVITAGVDESGIRVVLDDEGPGIPNIAQAMEKGYSTASQEVRDMGF
                     GAGMGLPNIRSNVDEFNISSVVNKGTVLDFFIRF"
     gene            874..2214
                     /locus_tag="PLI69_06050"
     CDS             874..2214
                     /locus_tag="PLI69_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOG42082.1"
                     /translation="MAEGAHCRYYRGLEIITDLCVGCSHCMKVCPTEALRVKGGKAVI
                     HSDLCIDCGRCYRECPTNAIRVVDDDFDHIFAYKHRLLLVPSLFYSQFEDRIEMNEIN
                     RIIGSLGFTEVSPVELGVDYLIEEMNRYIEEAPADSRPLISSYCPAIVRLVQVRFPSL
                     VDNIMTLLPPLEITAQYYKSKYRDAGIPEEDLGIFYLTPCVAKIAAVKDPVGGYVSPI
                     TGAINMDLLYSKVMMVHRNHSCTENNIRINEEITSNAIRWSTTRGETNAIRGVALAID
                     GMDNVIEFLEKVENGDIEGVDFLELRCCDESCSGGILVRGNRFLVADGLHKRASTAPW
                     VNPYIKDFRKYCADYMVFSRVQPRAFMKYGRDIKEAIRKMEEVRKLKHTLPGIDCGAC
                     GSPNCEALAGDIVNGERNINNCVFMQIANERRGTLKVDEAIALMEEIWGKRYDY"
     gene            2204..2536
                     /locus_tag="PLI69_06055"
     CDS             2204..2536
                     /locus_tag="PLI69_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOG42083.1"
                     /translation="MTIRQLVQEMNLQVFSGENGLDREIEGGYVSDLLSDVMGFSQEG
                     EVWVTLQTHKNIMAVASLKDLAAIVLVKGLKPDEDAMEVSNEEGLPILGTDKSCFEFT
                     GKLWSLIN"
BASE COUNT          691 a          521 c          669 g          717 t
ORIGIN      
        1 cagctttcgg acttgcatgt gctcaaacag cagttgcatt tttcatttaa tgatgctata
       61 tttgtcgcct gacaaacata ataacagccc tttaattata tattttttta tgaaactaaa
      121 agagatagtc gcccttgttg aaggaaaggt tatatgcgga gagcaaaacc ttgacgagtc
      181 agttgagtat gccttttctt ccgatttgat gagtgatgtg ctaacactgc gaattaccga
      241 tttcctgctt gtgacgggac ttagcaacat tcagtccata aggacggccg aaatgtccga
      301 ccttaaatat attcttatcg tgcgtgacaa gaaggttacc caggatatga tagaactggc
      361 ggatgacaat gatatgacta tcatcaccac cgagttctcc atgtttaaga catccggtct
      421 gctctataac gcaggtctta agcctgtata ttgattatgg aacttgttta cacagttgaa
      481 ggaggcgatt tcaataaagc cggccatgct tcatcgcaga tcaagcgtgt actcaaacaa
      541 ctcggagtac cacctgatat aattaaacgt gtggttgttg cgatgtatga agctgaagtc
      601 aatattgtcg cccacgcgtg gcggggagtt atcaccgcag gtgtcgatga atccggtatc
      661 cgggtggttc ttgatgatga gggccccggt atccccaata tagcgcaggc tatggaaaag
      721 ggctattcca ctgcatctca ggaggtgcgt gacatgggct ttggagcagg aatgggacta
      781 ccgaatatac gttccaatgt ggatgaattt aatataagct cagtagtcaa caagggtacg
      841 gtcttggatt ttttcattag attctgataa tttatggcag agggtgcaca ttgtagatac
      901 taccgtggat tggagatcat tactgatctt tgcgtaggtt gttcacactg tatgaaagtg
      961 tgtcctactg aggcgttgag ggtcaagggt ggcaaggctg taatccatag tgacctctgc
     1021 atcgattgtg gccgttgcta tcgtgaatgt cccactaatg cgatacgtgt ggtagatgat
     1081 gatttcgacc atattttcgc ttacaaacac cgcttgttgc tggtgccaag cctcttctat
     1141 tcccagtttg aggataggat tgagatgaac gaaatcaacc gtattatcgg ctcgcttgga
     1201 tttacggagg taagtcccgt ggaactgggg gtagattatc tgatagagga gatgaatcgt
     1261 tatattgaag aggctccggc cgattccaga cctttaattt cctcttattg ccctgccata
     1321 gttcgcctgg tacaggtgcg tttcccttcg ttggtggaca acataatgac tcttttaccg
     1381 cccttggaga ttacagctca atattacaag agcaaatatc gggatgcagg tattccggaa
     1441 gaggatttgg gcatatttta tctcacaccc tgcgtggcaa agattgcggc ggtcaaagac
     1501 cctgtcggag gatatgtctc tccgattaca ggagccataa atatggatct gctctatagc
     1561 aaagtgatga tggttcacag aaaccatagt tgtacggaga ataacattcg tattaatgag
     1621 gagattacct caaatgccat ccgctggtcc accacccgcg gcgagaccaa tgccatacgt
     1681 ggcgtagctc tcgctataga tggtatggac aatgtgatag agtttttgga aaaggtagag
     1741 aatggggata tagagggagt ggactttttg gaactcagat gctgcgatga gtcctgttcg
     1801 gggggtatcc ttgtaagagg caaccgtttt cttgtagccg acggcctcca taagagggca
     1861 tcgaccgctc catgggttaa tccatacatc aaggattttc gcaaatattg cgctgattat
     1921 atggtattca gtagggtgca accacgcgcc tttatgaaat acggaaggga tataaaggag
     1981 gccatccgca agatggaaga ggtgcgtaag cttaagcata cgttaccggg cattgattgc
     2041 ggtgcctgcg gttctcccaa ctgtgaggct ctcgccggag atatcgtcaa tggtgagaga
     2101 aatattaaca attgcgtctt catgcagatt gccaatgaaa ggagaggcac gctgaaggta
     2161 gacgaggcca tcgctctgat ggaagaaata tggggcaaaa gatatgacta ttagacaact
     2221 tgtgcaagaa atgaacttgc aggtattttc gggagaaaat ggacttgaca gggaaattga
     2281 gggcggatat gtatcggacc tgctctctga cgttatgggc ttttcacagg aaggtgaagt
     2341 ttgggttact cttcagacac acaaaaacat aatggcggtg gcttctctta aagatcttgc
     2401 tgccatagtt ttggtcaaag gactgaagcc ggatgaggat gcgatggagg tttccaatga
     2461 ggagggcctt cccattctcg gtaccgataa atcctgtttc gaatttacgg gtaaactctg
     2521 gtctctcata aactaaccac cgtggagctt gggtcataca aaggggatct gcataatcac
     2581 acttttctct ccccctgc
//
LOCUS       DAOSIE010000212         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_34577_length_2592_cov_3.448955, whole genome shotgun sequence.
ACCESSION   DAOSIE010000212 DAOSIE010000000
VERSION     DAOSIE010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34577_length_2592_cov_3.448955"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLI69_06060"
     CDS             complement(<1..351)
                     /locus_tag="PLI69_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOG42084.1"
                     /translation="MNRRYYAVILAGGQGLRMGHDIPKQFIEIEGKPILRHTIERFRE
                     SFDEIEIIVVLPSGEKEYWKEYCKRTGFLDRYVMPSGGITRFHSVQNALKYVPDGAIV
                     AVHDGVRPFVTRDFL"
     gene            445..1953
                     /gene="purH"
                     /locus_tag="PLI69_06065"
     CDS             445..1953
                     /gene="purH"
                     /locus_tag="PLI69_06065"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOG42085.1"
                     /translation="MRTENYTRKIRRALISVYSKDGIEEIARELVACGVELLSTGGTF
                     TFLKELGLDVTAVEEITSYPSILGGRVKTLHPKVFGGILYRREHLQDIGEIEQYGIGP
                     IDLVIVDLYPFEKYVQMGATPEEIIEKIDIGGIALIRAAAKNYRDVIIASCKESYGPL
                     LHLLKSKQGESTIEERRSFARQAFHTSSYYDTQIFNWFNRDNAQSMPLRYGENPHQQA
                     KFTGSSESLPKQLHGKEISYNNLLDIEAALELMYDFPEPTVAIIKHNNACGCASRDTI
                     LEAWKDALAGDPVSAFGGIIVANRIIDEESAQEINKLFFEVIIAPEYSHEALEILKSK
                     KNRIILLDNGVTPSAKKFRSLLGGILVQDRDEKVESPEELKTVTTLAPTPGQVEDMLF
                     ANKLVKHSKSNAIVLAKDSMLLASGIGQTSRVDALRQAIAKAKSFGFDLNGAVMASDA
                     FFPFPDCVEIAHEAGIVAVIQPGGSVRDQESIDWCNANGMAMVTTGFRHFKH"
     gene            1978..>2592
                     /locus_tag="PLI69_06070"
     CDS             1978..>2592
                     /locus_tag="PLI69_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG42086.1"
                     /translation="MIFSFLTQELAIDLGTANTVIFMNDKKIIDEPSIVAVDNLTGKV
                     LAVGTKAKQMQGKESKGVRTIRPLKEGVIADFDSSEQMIREFIKMINTRRSFFSPSLK
                     MVVCIPSGSTPVETRAVRDAAEHSGGRGVYLIFEPMAAALGVGIDVTAPSGNMIVDIG
                     GGTTEIAVISLGGIVADSSIKVAGDQFTNEIQQYMRQQHNIKIGE"
BASE COUNT          747 a          592 c          602 g          651 t
ORIGIN      
        1 aagaaaatcc cgcgtgacaa aggggcggac cccgtcatgg acagctacta tcgccccatc
       61 cggaacatat ttaagggcat tctgaaccga atgaaacctg gtgatgccac ctgagggcat
      121 tacatagcga tccaaaaaac cggtacgctt acaatactcc ttccaatact ccttctctcc
      181 cgaaggcaac accacgatga tctcgatctc atcaaagctc tccctaaagc gttcaatggt
      241 gtgtcgaaga atcggtttcc cctcaatttc aatgaattgt ttaggaatat catgtcccat
      301 tctcaacccc tgtcctccgg caagtataac ggcataatag cgtctattca ttccctttaa
      361 aaattatact gcaaaaatag ccgaaaatcc cgttattatt actaaatttg cttgatattt
      421 ttcatcttta ccatattccc gtaaatgcgt accgaaaatt ataccagaaa gattagaaga
      481 gctctgattt ccgtttacag caaagatggc atagaagaga tagcccgcga acttgttgcc
      541 tgcggggtgg aattgctctc taccggcgga acattcacat ttctcaaaga gctggggctg
      601 gatgtcactg cggtggaaga gatcacctct tatccttcca tcctgggagg tagggtaaag
      661 acacttcatc caaaagtatt cggaggtatt ctctatcgga gagagcatct acaggacatt
      721 ggagagatcg agcagtacgg aataggaccc attgatctgg tgattgtcga cctctatccc
      781 tttgagaaat atgtacaaat gggtgcaact cccgaagaga taattgaaaa aatagatata
      841 ggaggtattg cgcttattag agctgccgca aaaaattacc gtgatgtaat catagcctca
      901 tgtaaggagt cctatgggcc gctactccac cttctaaaga gtaaacaggg cgagagtacc
      961 atagaggagc gcaggtcatt tgccagacag gcattccata cctcctctta ttacgacacc
     1021 caaatattta actggtttaa cagggataat gcccaatcaa tgcccctgcg ctatggtgaa
     1081 aaccctcacc agcaggcaaa gtttaccggc tcaagtgaat ccctgccaaa acagttgcat
     1141 ggtaaggaga tttcctataa taatctactt gacatagagg cggcattgga gcttatgtat
     1201 gacttcccgg agcctacggt agccataata aaacataaca acgcttgcgg gtgtgcttca
     1261 cgcgatacta tattggaggc atggaaagat gctcttgcag gtgatcccgt atctgccttc
     1321 ggtggcatta tcgtagccaa cagaattata gatgaggaat ctgctcagga aatcaacaaa
     1381 ctcttttttg aagttattat agctccggag tactcccacg aagctctcga aatattgaaa
     1441 tccaagaaaa accggattat cctccttgat aacggggtaa ctccttcggc taagaagttc
     1501 cgttctttgc tagggggcat tctcgttcag gatagagacg aaaaggtaga gagtcccgaa
     1561 gagcttaaaa ccgttactac tcttgcgcct actccggggc aggttgagga tatgcttttt
     1621 gccaacaaat tggtaaaaca ttccaagtcc aacgccatag tgctggccaa agactccatg
     1681 cttcttgcca gcggtattgg gcagacctcc cgagtggatg cattaaggca ggcaatagcc
     1741 aaagctaaat ccttcggatt tgacctcaac ggggcagtaa tggcttccga tgccttcttc
     1801 ccttttcctg attgtgttga aatagcacac gaagcaggaa ttgttgcagt aatccagccc
     1861 ggaggctctg tcagggacca ggagagcatc gactggtgta acgcaaacgg catggccatg
     1921 gtcaccaccg gtttcagaca tttcaaacat taataaagat atagttttat aagatatatg
     1981 attttttcat ttttaacaca ggaactcgct attgaccttg gcaccgccaa tacggtcata
     2041 tttatgaatg acaagaaaat catcgatgag ccctccattg tcgccgtaga caatctgaca
     2101 ggaaaagtcc ttgcagttgg cacaaaggct aaacagatgc agggtaaaga gagcaagggt
     2161 gtgaggacta tcaggccgtt aaaggagggt gtgattgcag atttcgacag ttctgagcag
     2221 atgataaggg agtttatcaa gatgataaac acccgcaggt ccttcttttc accaagtctc
     2281 aagatggtgg tctgcatccc ttccggcagt actccggttg agacgcgtgc ggtaagagat
     2341 gctgcagagc attcgggcgg acgcggcgta tatcttattt tcgagccgat ggcagctgcc
     2401 ctcggagtag gaatcgatgt gactgcccct tcgggaaata tgattgtcga tataggtggc
     2461 ggcactactg agattgccgt aatttcactt gggggaatag tggccgactc cagcattaag
     2521 gtggccggag accagtttac caacgaaata cagcaatata tgcgccagca acacaatata
     2581 aagattggag aa
//
LOCUS       DAOSIE010000213         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_34719_length_2583_cov_2.686313, whole genome shotgun sequence.
ACCESSION   DAOSIE010000213 DAOSIE010000000
VERSION     DAOSIE010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34719_length_2583_cov_2.686313"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..268
                     /locus_tag="PLI69_06075"
                     /pseudo
     CDS             47..268
                     /locus_tag="PLI69_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            274..2040
                     /locus_tag="PLI69_06080"
     CDS             274..2040
                     /locus_tag="PLI69_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOG42087.1"
                     /translation="MDKIKLTIDNKPVEVAKGTTILQAARQVGIKIPTLCYMNLEGMG
                     VENRPGACRICVVEVEGRRNLAPSCATDCTEGMVVRTTTPKVLNARRTVMELILSNHP
                     KDCLVCASNGDCELQDLAATLGIREIRMVENAAVSTYRKDYSFSLKRDMDKCIICRRC
                     ESMCNDVQTVGALGAINRGFTSVVATAFEQNLSDSPCVFCGQCVAVCPTGALTRTDDT
                     GKVFKALADPTKTVIVQTAPAVRAALGEEFGLEPGTLVTGKMAAALRRLGFDKVFDTD
                     FGADLTIVEEGTELLGRIGAFLNGDTSIKLPIFTSCCPAWINFFEQNYPDMLDQPSTA
                     KSPQQMFGAIAKNYFAKKSGIKREDLVVVSVMPCIAKKYELTRNEFSSDGNRDVDISI
                     STAELASMIKMANIDFMSLPDEDFDHPLGESTGAAVIFGTTGGVMEAAVRTAYEVFTG
                     KTLERLDFKEFRGFEGIRTASVDFNGTAINICIAHQLGNARKVMDGIRKGELSFHAVE
                     VMACPGGCIGGAGQPYHKGNASILKARAQSLYREDAAKPLRKSHENPYIMKLYEEFLG
                     QPMSELAHHLLHTHYFDKHHKI"
     gene            2044..2517
                     /locus_tag="PLI69_06085"
     CDS             2044..2517
                     /locus_tag="PLI69_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG42088.1"
                     /translation="MKIELKDSQIRQIKDICNSLNNDPGELITVLHRTQNLFGYLPEE
                     VQRVVAHNLGISAAKVFGVVSFYSFFTMTPKGKYPISLCMGTACYVRGAENVLDEFKK
                     ELGIEVGGVTPDGKFSLDCLRCVGACGLAPVVMIGEKVYGRLSPSMVKEVLAEYK"
BASE COUNT          664 a          559 c          659 g          701 t
ORIGIN      
        1 ctcttaagag ccttacactg tccggcagga cattttgtat cgtgctcacg tggtagataa
       61 aaaatgtcct gccggacagt gtaaggctct taagagttac attatcgatc ctacaaagtg
      121 tatcggatgt accctctgcg cccgcaattg tcctactgat gccatcaccg gcgacaaaaa
      181 agtacctcac gttatcgacc agtcgaaatg catcaagtgc ggtgcctgct atgaaaaatg
      241 taagtttggt gcaattctaa tacgctgata attatggata aaataaaact cactatagat
      301 aataagcctg tggaggtagc taaaggaacg accattttgc aggctgcacg ccaagtgggc
      361 attaagattc ctactttgtg ctatatgaat ctggagggca tgggtgttga aaaccgcccc
      421 ggtgcctgtc gcatttgtgt tgtggaggtt gagggacgtc gtaatctggc tccttcctgt
      481 gccaccgatt gtaccgaggg tatggtagta agaaccacca ctccgaaggt gctgaatgct
      541 cgcagaaccg ttatggaact catcctctcc aaccacccca aggactgtct cgtatgtgcc
      601 tccaacggtg actgcgagtt gcaggatctc gctgctactc tcggtataag agagatacgc
      661 atggttgaaa atgctgcggt ctctacttac cgtaaagatt actcgttttc tctgaagaga
      721 gatatggata agtgtatcat ctgccgtcgt tgcgagtcca tgtgtaatga tgttcagaca
      781 gttggtgctc ttggagcaat caaccgcggc tttacgtcgg tagtcgctac tgctttcgaa
      841 cagaatcttt cggattcacc ctgtgtattc tgcggtcagt gcgttgcagt atgccctaca
      901 ggagctctca ccaggacaga tgacaccggt aaagtattta aggcacttgc agatcctacg
      961 aagactgtaa tcgtacagac tgctcccgct gttcgtgcag cactgggaga ggaatttgga
     1021 cttgagcccg gtactcttgt taccggcaag atggctgctg ccctgcgtag gctcggtttt
     1081 gataaggtat tcgataccga tttcggcgcc gaccttacaa ttgtggagga gggtacggaa
     1141 ctcctcggac gtatcggagc cttccttaat ggcgatactt cgatcaagct gccgatattt
     1201 acctcttgct gtcctgcctg gatcaatttc tttgaacaga attatcccga tatgctggat
     1261 cagccttcaa ccgccaaatc acctcagcag atgtttggag cgatagcaaa gaattatttt
     1321 gccaagaagt ccggcatcaa gagggaggat ctggttgtgg tatccgtgat gccttgtatt
     1381 gctaagaaat atgagcttac aagaaatgag ttttcatcgg atggcaaccg cgatgtcgat
     1441 atctccatct ccactgcgga gcttgcatcc atgataaaga tggcaaatat agatttcatg
     1501 tcgctgcctg atgaggactt tgaccatcct cttggagagt cgaccggagc tgccgttatc
     1561 ttcggaacca ccggaggtgt gatggaagct gccgtgcgta ctgcttatga ggtatttacc
     1621 ggcaagaccc tcgaacgcct cgatttcaag gagttccgtg gctttgaagg tattcgtacg
     1681 gcaagtgtgg atttcaacgg tactgctata aatatttgta ttgcacacca gctgggtaat
     1741 gcccgcaagg tgatggatgg tatccgtaaa ggagagctca gcttccatgc agttgaggta
     1801 atggcttgtc ccggaggttg tatcggcgga gccggacaac cttatcataa gggtaacgca
     1861 tcaatactaa aggcgagagc acaatcactc tatcgtgagg atgccgcaaa gcctttacgt
     1921 aagtctcacg agaatcctta tattatgaaa ctctatgagg agttcctggg tcagccgatg
     1981 agcgaacttg cacaccatct gctccataca cactattttg acaaacacca taaaatttag
     2041 gatatgaaaa tagaattaaa agatagccaa attaggcaga ttaaagatat atgcaactcg
     2101 ttgaataatg atccgggtga gctgattaca gttctgcata ggacacagaa tctgtttgga
     2161 taccttcctg aagaggtaca aagggttgtg gctcataatc ttggtatttc tgcagcaaag
     2221 gtttttggtg ttgtgagctt ctattcattc ttcacgatga ctcccaaggg taaataccct
     2281 atatcgctct gtatgggaac agcttgctat gtacgtggag ctgaaaatgt gttggatgag
     2341 tttaagaaag agttgggaat agaggttggc ggagtgactc ccgatggaaa attctcactg
     2401 gactgcctac gttgtgtagg agcttgcgga ctcgctcccg tggttatgat aggggagaag
     2461 gtttatggcc gtctcagtcc ttccatggta aaggaagttc ttgcagagta taaatagatg
     2521 aagggacgtc aggtacgtct ctgattgata cttgatttgt ggccggatga tttgacaacg
     2581 aca
//
LOCUS       DAOSIE010000214         2575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_34851_length_2575_cov_3.461905, whole genome shotgun sequence.
ACCESSION   DAOSIE010000214 DAOSIE010000000
VERSION     DAOSIE010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34851_length_2575_cov_3.461905"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLI69_06090"
     CDS             <1..405
                     /locus_tag="PLI69_06090"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG42089.1"
                     /translation="GIREVLSIFGDDYDTPDGFCIRDYIDINDLAAAHVAALDRMIGS
                     KMKESYEVFNIGTGRGVSTMEIVKTFEKVNDLNLNYRITGRRAGDIEAMWADPTYANE
                     ELGWYARKSLYETLRSAWKWQQRLSGLEISKD"
     gene            complement(437..1858)
                     /locus_tag="PLI69_06095"
     CDS             complement(437..1858)
                     /locus_tag="PLI69_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG42090.1"
                     /translation="MTGPLDHKIFEVVATQAAKLKLKAFVIGGYVRDHFLQRESTDID
                     IVVEGSGIALAEAVAEVTHTNVTVFRNFGTAMLKYEGIELEFVGARRESYRRNSRKPV
                     VEEGTLEDDQLRRDFTINALAFSLGKEDYGTLIDPFGGIRDLDAGLIRTPADPDTTYS
                     DDPLRMIRAIRFATQLGFKIVQESIDSIRRNAGRLDILARERIAEELHKILMAEIPST
                     GLRLLDECALLPLILPAISNLKSVEMVNGCGHKDNFEHTLQVLDNVASAGGDLWLRWA
                     ALLHDVGKPATKKFEAGTGWTFHGHEYVGSKMVPGIFKQLKMPLNDKMKFVQKMVLLH
                     LRPIALVTEEVTDSAVRRLLFDAGDDIDDLMTLCRADVTSKNEYKVKAIHRNFEIVEA
                     KLIEVEAKDALRNFKNPITGELIMEKYGLPPCATIGVIKDYIKNAILDGEIPNEFEAA
                     YRLMESKAAEMGLEPKSQSFEKK"
     gene            complement(1855..2250)
                     /locus_tag="PLI69_06100"
     CDS             complement(1855..2250)
                     /locus_tag="PLI69_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOG42091.1"
                     /translation="MQVFDPLRKKYVRLTPEEEVRQNVIAWLAQEWGVPLPLMSSEWA
                     FSFNGMQYRADVLVFDRRLQPLMLVECKAHDIPIDRTVVDQVIRYNLSLKVKYILITN
                     GKSSYLCGRNPKTGQYEFLDRIFKYEEML"
BASE COUNT          720 a          626 c          527 g          702 t
ORIGIN      
        1 ggtatacgtg aggtgttgag catcttcggg gacgattacg atactcctga cggattttgc
       61 atccgcgact atattgacat caatgatctg gcagctgcgc acgtagctgc gctagacaga
      121 atgataggct ccaaaatgaa agagagctat gaggttttca atataggtac gggacgcgga
      181 gtctccacga tggaaatcgt aaaaaccttc gaaaaggtta acgacttgaa cctcaactac
      241 cgcataaccg gcagaagagc gggcgatatc gaagctatgt gggcagaccc cacctacgcc
      301 aacgaagaac ttggctggta tgcacgcaaa agtctctacg aaactctccg ctcagcctgg
      361 aagtggcagc agcgtcttag cggactcgaa atttcaaagg attagtttta agcaagggtc
      421 agctcttctc ggagacttac ttcttctcaa atgattggct tttcggctcc agacccattt
      481 cagcggcctt agactccatc agacgataag ctgcctcaaa ttcattaggt atttctccgt
      541 ccaaaatggc attcttgata taatctttaa taacaccgat tgtggcacaa ggcggcaggc
      601 catatttttc cataatcaat tcgccagtga tggggttctt gaagttacgg agggcatctt
      661 tggcctcaac ctctatcagt ttagcctcaa caatttcaaa attacggtga atcgccttga
      721 ccttatactc attcttagaa gtaacatctg ctcgacagag agtcatcaga tcatcaatgt
      781 catctcccgc atcaaagagc agacggcgca ctgctgaatc tgtcacctct tcggtaacca
      841 gagctatggg cctaagatga agcagaacca tcttctgcac aaatttcatc ttatcgttta
      901 acggcatctt gagctgctta aatatacccg gaaccatctt actacccacg tattcatgtc
      961 cgtgaaatgt ccatccggta cctgcttcaa actttttggt ggcgggcttg cccacatcgt
     1021 gaagcagcgc agcccaacgc agccagagat ctccacccgc agatgccaca ttatccaata
     1081 cctgaagagt atgctcaaag ttgtctttgt gaccacaacc attaaccatc tctacagatt
     1141 tcaaattgga aatggcaggg agtatcaagg gcaaaagtgc acattcatca agcaggcgca
     1201 ggcctgtgga gggaatctcc gccatcaata tcttgtggag ttcctcggct attctctcac
     1261 gggcaaggat atcgagacgt cctgcattgc gacgtatgga gtcaatagac tcctgaacta
     1321 ttttgaagcc taattgtgtg gcaaatctta tcgctctgat catccgcagg ggatcgtctg
     1381 agtaggtagt gtccggatcg gcaggggtac gtataaggcc ggcatccaaa tcccggatac
     1441 cgccaaaagg gtctatcaaa gtaccgtaat cttctttgcc gaggctgaaa gccagagcat
     1501 ttattgtaaa gtcacgtctt aactgatcat cttcgagggt accctcttcg actactggtt
     1561 tcctggaatt gcgacggtat gattcgcgtc ttgcaccgac aaactccagt tctatcccct
     1621 catatttgag catcgccgta ccaaaattac ggaagactgt gacatttgta tgagttacct
     1681 ctgcaaccgc ctctgccagc gcaataccgc tgccctccac tacgatatct atatcggtac
     1741 tctctctctg aagaaaatga tcacgcacat aaccgccaat aacgaaagct ttgagcttaa
     1801 gtttggcagc ttgtgttgct actacctcaa aaatcttatg gtcaagtggc ccggtcataa
     1861 catctcttca tatttaaaaa tcctgtcgag aaattcatat tgcccggttt taggattacg
     1921 gccacaaaga taactgcttt tgccgtttgt tatcaaaatg tacttcactt tgagcgaaag
     1981 attgtatctg ataacctggt ctaccaccgt cctgtcaata gggatatcgt gagctttgca
     2041 ctccaccagc ataagaggct gcaacctacg gtcaaagacc agcacatctg ccctgtactg
     2101 cattccgtta aaagaaaatg cccattcact ggacataagc ggaagaggta ctccccattc
     2161 ttgcgccagc caggcaataa cattttggcg tacttcctcc tcaggtgtga gcctgacata
     2221 ctttttcctc aatggatcaa aaacctgcat ttatttccta tctttaatgc gaagatagca
     2281 tttttcatta aatttaatta gacgtttcct ttcgaattac caagataaag tccggtccga
     2341 gatattacca ctgacaattg gaaataaaag ttgagggtgg cacttatgcc accctctaaa
     2401 aattcaaact tttgtttcct accaaattcc ccattggtag gttacaaagg taattgtaag
     2461 aattttaatt tacaaatttc taaaattgac agaattctgt tttgcaataa ttgccaattt
     2521 ccaaaaaact acaatgaata gttattataa cgacctgtct tacagtatat tacaa
//
LOCUS       DAOSIE010000215         2572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_34911_length_2572_cov_3.812078, whole genome shotgun sequence.
ACCESSION   DAOSIE010000215 DAOSIE010000000
VERSION     DAOSIE010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34911_length_2572_cov_3.812078"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2518
                     /locus_tag="PLI69_06105"
     CDS             <1..2518
                     /locus_tag="PLI69_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG42092.1"
                     /translation="EIAEIIMSKPFPNVTLSPDYKRGVIAERESALVPLATLAAEELR
                     IGGMRINPRNFTQTRESFFNSVTILDVATGGLTQIEEMPADAKIREIKWSPSGKYFCF
                     LNETPEEMALWRVNVSDPTPKAVKINKYKVNSTLSNGNTYEFLDDERIIYKSVPENIG
                     PVPTPTLPNGPVVQENIGDRKSVRTFQDLLSSPFDKVLYDYYCTVVFAIYDNGNTSTA
                     GPAGVVREYDISPDSRYMMVTTEQHPYSYMEGHNSFPSIREIRTISGETVVILSDTNK
                     EKAEKELRDKAPKDTSATGIPEPPRKLNFEWRADMPATLVWTEGSSGPSPFMAPNPDA
                     DNKEDEPEKKFTDKVYQCPEPFNFDDKQLVVAPEYKIGRITWGNPQLAVYTESSQKQK
                     FRRTVAFTPCDTIAPKQILFTESTDVDTLGNFPVYGRFWTVNNQYGKKVLYTDSKNSH
                     LFLNDTRRPDAEGDNFSFIDRITIKNGKIENLWNGAAPYQERVIAITDFDRLKFISER
                     QSISEVPNYYAVDTRNKKNSHRQFTFFENPVPMLSGVTRNFVTYKRADGVTLSANLWL
                     PAGYDQERDGKLPVLMWGYPYEYKCAAEAQKSRPARYNFLRPGYGSAVLWVTQGYAVM
                     DGISMYIISENKEKEPNDVFLSQLIMNAEAAINFVDSIGVGDRDRVAIGGHSYGGFMT
                     ANLLAHTDLFKAGIARSGAYNRSLTPFGFQSEKRNYWKAKEVYDKMSPFNYADKLKTP
                     ILIIHGQMDDNSGTFPIQSERLYHAVAGHGGTARYLQLPYESHGYSAYENVMHMLYET
                     DAWMAKWLSPEEVAKVKERQQKEKEKKDKAKDKDKDKKDE"
BASE COUNT          783 a          623 c          584 g          582 t
ORIGIN      
        1 ggagatcgca gaaatcatca tgtccaaacc ctttcccaat gttacactca gcccggacta
       61 taagcgcggt gtgatagcgg agagagagtc tgcgctggta ccacttgcaa ctctcgctgc
      121 agaagagcta cgtatcggag gcatgcgtat caatccccgt aatttcacac aaactcgtga
      181 gtcattcttc aatagtgtca ctatattgga tgttgccaca ggaggcttga ctcaaataga
      241 ggaaatgcct gcggatgcga agatccgcga gatcaaatgg tccccctccg gaaaatactt
      301 ttgtttcctt aacgaaactc ccgaggagat ggcactttgg cgtgtaaatg tttccgaccc
      361 cactccgaaa gcagttaaaa tcaataaata caaagtcaat tccacccttt ccaacggaaa
      421 tacatacgaa tttctggatg atgagagaat aatatacaaa agcgtacctg aaaacatcgg
      481 tcccgtaccg acgccaaccc ttcccaatgg tcccgtagta caggaaaata ttggggatag
      541 aaagtctgta aggacatttc aggacctact ctcctcacct ttcgacaagg tgctctacga
      601 ctattactgc acggtggtat ttgccatata tgataacggc aatacctcta ccgcggggcc
      661 cgcaggagta gttagagagt acgatatctc tcctgactcc cgctatatga tggtcaccac
      721 cgaacaacac ccctactctt atatggaggg acacaattca ttcccgagca ttcgagaaat
      781 ccgtacaatc tccggggaga ctgtggtgat actcagtgac acaaataagg aaaaagctga
      841 aaaagaacta cgcgataagg ccccgaaaga cacttcagcg accggcatac ccgaaccccc
      901 gcgcaaactc aacttcgaat ggagagcaga tatgccggct actctggtat ggacggaagg
      961 tagctcaggc ccttcaccct tcatggcacc caatccggat gcagacaata aggaagatga
     1021 gcccgaaaag aagtttaccg acaaagtata ccaatgtccg gaacccttca acttcgatga
     1081 taagcaactt gtagtggctc cggaatataa aataggacgc attacctggg gaaatcccca
     1141 acttgcagtc tataccgaga gttcacagaa acaaaaattt cgcaggacgg tagcattcac
     1201 tccttgcgac actatagcgc cgaaacagat actctttacg gaaagcacgg atgttgacac
     1261 ccttggcaat ttccccgttt acgggcgctt ttggactgtc aacaaccaat atggcaaaaa
     1321 agtgctctat accgatagca agaactcaca tctcttcctc aacgatacca gacgtcctga
     1381 tgcggaaggt gacaacttct ctttcataga taggataacc atcaaaaatg gtaaaattga
     1441 gaatctttgg aacggagctg ctccatatca ggagcgcgta atcgccataa cggatttcga
     1501 ccgtcttaaa ttcatctccg aacggcagtc catcagcgaa gttccaaact actatgccgt
     1561 ggatacaaga aataaaaaga atagtcacag acagtttacc ttctttgaaa atcctgtgcc
     1621 tatgctaagt ggggtaacga gaaacttcgt gacctataaa cgtgctgacg gcgtgacact
     1681 aagtgccaac ctctggctac cggctggtta tgaccaggag agagatggga aactgcccgt
     1741 actgatgtgg ggctacccct acgaatataa atgcgctgcc gaagcgcaaa agagccgccc
     1801 tgcaagatac aatttcctca ggcccggcta tggctctgca gtactgtggg ttacgcaagg
     1861 gtatgctgtg atggatggca tttcgatgta tataatctcc gaaaataagg agaaagaacc
     1921 caatgatgtt ttccttagcc agcttataat gaatgcagag gcggccatca atttcgttga
     1981 ctccatcgga gttggtgaca gggatagggt tgcgatcggt ggtcactcct atggtggatt
     2041 tatgactgcc aacctcttgg ctcacaccga cctgttcaaa gccggcattg cgcgtagcgg
     2101 tgcctacaac aggtctctta ccccgtttgg cttccagagt gaaaaaagaa actactggaa
     2161 agcaaaggaa gtttatgata aaatgtctcc cttcaattat gccgataaac tcaaaactcc
     2221 catcctgatt atccacggcc agatggatga taatagcggc acattcccca tacaatccga
     2281 gcgtctttat catgcagttg caggccatgg cggaacagcc agatacctcc aactcccata
     2341 cgagagccat ggatattccg cttatgaaaa tgtgatgcac atgctctacg aaacagatgc
     2401 ctggatggca aaatggctct cacccgaaga ggtggctaag gtaaaggaga gacagcagaa
     2461 ggagaaagag aaaaaggata aggcaaaaga taaggataag gacaaaaagg acgaataatc
     2521 ggttttttta tatctttgcg gcgaaatttt tacaatcaat tatggcagac aa
//
LOCUS       DAOSIE010000216         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_35266_length_2551_cov_3.272837, whole genome shotgun sequence.
ACCESSION   DAOSIE010000216 DAOSIE010000000
VERSION     DAOSIE010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35266_length_2551_cov_3.272837"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLI69_06110"
     CDS             <1..287
                     /locus_tag="PLI69_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOG42093.1"
                     /translation="GLQFALSGEKKSEISDRLAGATIVISGNFSISREEMKSLIAAHG
                     GKNTGSVSGKTTYLLAGDKSGPEKLKKAGKLGIRVITEEELLKMIEGELT"
     gene            305..742
                     /locus_tag="PLI69_06115"
     CDS             305..742
                     /locus_tag="PLI69_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42094.1"
                     /translation="MKELYRIIFKFLTRDIWRLDFSEFGKARRRAIRYLKALIITFRG
                     FSGQRIAREAVALSYFGLMAIVPIVALILFVASGFGLDKMLYELLYSSFPNSSQMIDA
                     VLNFANNIMDKFRGICLAGNLVDDQCRVGIQSHLGGKGVYRYR"
     gene            678..1274
                     /locus_tag="PLI69_06120"
     CDS             678..1274
                     /locus_tag="PLI69_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOG42095.1"
                     /translation="MINVESAFNRIWVVKEYTGIGKRIVAYIAILILSPFMIILFLYG
                     WGYYIRFLSSVSENLGPFSFLTTNLFWVVFYGVAVLVFSLLYKFIPHTRVRYSSAIKG
                     AIVSGVAFVGIQYLYLNTQLMVTRLSGVYGALAAIPLLMIWMNLCWGVILFGSQLSYG
                     FQQVDYEHPVNPIGAEDSSDEKEIILEQHIKPITKNGK"
     gene            1264..>2551
                     /gene="hydG"
                     /locus_tag="PLI69_06125"
     CDS             1264..>2551
                     /gene="hydG"
                     /locus_tag="PLI69_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOG42096.1"
                     /translation="MENKYKNFIDEDLIHSLIRETKEDKEAIRAIFAKSKAKEPLTLS
                     ETAQLLAIESDEMKEELYETARELKRAIYGNRIVLFAPLYIGNHCINDCKYCGFRRSL
                     RTAVRKTLSDQELVNEVINLENIGHKRLILVYGEHPMYSPEFIAHTVRLCYQTKVGNG
                     EIRRVNINAAPQDVEGYRIIKEAGIGTFQVFQETYHQETYARVHPKNTHKGDYFWRLH
                     AMDRAFEAGIDDMGIGALFGLYDWRFEVLGLVAHAIHLKETHGVGPHTISFPRIQPAH
                     GMDLDLPWRVTDEQFKMLVAILRLSVPYTGLIMTARESSQVRDDVIDFGVSQIDAGTK
                     LEIGGYQKEEYEQQLDKEQFRIGDTRDLDQVIRWLLTRGYIPSFCTSCYRLGRTGEHF
                     MEFAIPGFIGNFCTPNAIITLAEYLEDYASEETKKVG"
BASE COUNT          704 a          581 c          622 g          644 t
ORIGIN      
        1 tcggacttca atttgccctc tccggggaga aaaagagtga gatttccgat cggctcgcag
       61 gagctaccat cgtgatttcg ggtaattttt ccatttcccg ggaggaaatg aaatctctca
      121 tagccgccca cggcggcaaa aataccggct ccgtcagcgg aaagactacc tatctccttg
      181 ccggcgacaa gagcggaccg gaaaaactta aaaaagccgg gaaactcggc atacgggtga
      241 taaccgagga ggagctcctc aaaatgattg aaggagagtt gacatgaggt tgtttataca
      301 aagaatgaaa gagctttacc ggatcatctt caaatttctt acccgggaca tctggagact
      361 ggatttcagc gagtttggaa aggcccgcag gagagccata cgctatctca aggcactgat
      421 tatcactttc aggggttttt caggacagag gattgcacgg gaagccgttg cgctgagcta
      481 tttcggcttg atggccatcg taccgattgt tgcgctgata ctctttgtag ccagcggttt
      541 cggactcgac aaaatgctct acgagttatt atactcctct tttccaaaca gttctcagat
      601 gattgatgca gtgctcaact ttgccaataa catcatggat aagtttcggg gcatttgcct
      661 ggctggtaat ctggttgatg atcaatgtcg agtcggcatt caatcgcatc tgggtggtaa
      721 aggagtatac aggtataggt aagaggatag tagcttatat agccattctt attctctccc
      781 ctttcatgat aatccttttc ctatacgggt ggggctatta tatcagattt ctctcatcag
      841 tcagcgaaaa tctcggcccc ttcagtttcc ttaccacaaa cctgttctgg gtagtctttt
      901 acggagtagc cgtattggtt ttctcccttt tatataaatt cattccccat actagggtaa
      961 gatacagctc tgccataaag ggagcaatcg tttcaggcgt ggcatttgta ggtatacaat
     1021 atctctatct aaacacccag ttgatggtga cacgactgag cggtgtttac ggagcgcttg
     1081 cagcgatccc gctactgatg atctggatga atctctgttg gggagtaatc ctcttcggat
     1141 ctcaactttc gtatggattt cagcaagtgg actatgagca tccggtaaat cccattgggg
     1201 cggaggatag cagtgacgaa aaagaaataa tattagaaca acatatcaaa ccaataacaa
     1261 aaaatggaaa ataaatacaa aaattttatt gacgaagatc ttatccattc ccttataagg
     1321 gagacaaagg aggataagga ggcaatcaga gccattttcg ccaaaagcaa ggcaaaagag
     1381 cccctgaccc tctcggagac tgcccaactg ctcgcaatag agtcagatga gatgaaagag
     1441 gagctttatg agactgcaag agagctcaaa agggctattt acggcaacag aatagtactc
     1501 ttcgcacccc tttatatagg taaccactgc atcaatgatt gtaaatattg cggtttccgc
     1561 cgctcgctac gcaccgcagt tcgtaaaaca ctgagcgacc aggaattggt caatgaggtg
     1621 atcaatctag aaaatattgg tcacaagcgc ttgatcctcg tatatggaga gcaccctatg
     1681 tactcacccg agttcattgc gcatacggta agactctgct atcagactaa ggtaggtaat
     1741 ggagagatac gccgtgtaaa tatcaatgcc gcccctcagg atgtagaggg ctataggatc
     1801 ataaaagagg caggcatcgg cacttttcag gtatttcagg agacctatca ccaggaaact
     1861 tatgcgaggg tacaccctaa aaacacccac aagggcgact acttctggcg tcttcacgct
     1921 atggaccgcg catttgaagc aggaattgat gatatgggta tcggagctct gttcggactc
     1981 tacgactggc gcttcgaggt attgggactc gtagctcacg caatccatct caaggagact
     2041 catggggtgg gtcctcacac catcagtttt ccccgcatcc aacccgccca tggtatggat
     2101 ttggatctcc cctggcgcgt aacagatgag cagtttaaga tgctggtggc cattctgcgt
     2161 ctctccgtac cttatacggg actaattatg accgcccgcg aaagcagtca ggtaagggac
     2221 gatgtgatag atttcggagt atcccaaata gatgccggca ccaaactgga aattggcggc
     2281 taccaaaagg aggaatatga gcagcaacta gacaaggaac agttccggat tggcgatacc
     2341 cgcgacctcg atcaggtgat caggtggctc cttaccaggg gatatattcc cagtttctgc
     2401 acctcttgct accgtttggg acgtaccgga gagcatttta tggaatttgc cataccggga
     2461 ttcatcggaa atttctgtac ccccaatgcc attatcacac ttgcagaata tctcgaggat
     2521 tacgcttcgg aggagacaaa gaaggtggga t
//
LOCUS       DAOSIE010000217         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_35391_length_2544_cov_2.719566, whole genome shotgun sequence.
ACCESSION   DAOSIE010000217 DAOSIE010000000
VERSION     DAOSIE010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35391_length_2544_cov_2.719566"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLI69_06130"
     CDS             <1..443
                     /locus_tag="PLI69_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOG42097.1"
                     /translation="TGYELMVQDYNAFEVGSTVGLVIRPEDIQVMHKERSRNTFEGEM
                     VDENHVRFLDGEFECPIQPGVEKGQKVKVEVEFDKVDLYDHQEEGTLSGSVHFMIYKG
                     NHYHITVLTDEGHHIWVDTNDVWDKGDLVGINFPAKHIKITQLQ"
     gene            440..1207
                     /locus_tag="PLI69_06135"
     CDS             440..1207
                     /locus_tag="PLI69_06135"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG42098.1"
                     /translation="MNKRSSWSLPYLIFLVIFVVLPLILVLVYAFQDAQGRFSFENIT
                     RFFSDNDALSTFGLSLEIAILNTLICLLLGYPAAWILANKKYNRSAVTVVLFILPMWI
                     NALMRTLATAELFNVLGITLGKGTLLFGMFYDYLPFMIYPIYNILSRIDHSYAEAAED
                     LGATPWEVFWKVTVPLSIPGVISGVMMVFMPTVSTFAISEFLTNNKIKLFGTIIQENI
                     NSSMWNYGAALALIMLVIIGITTFFSNDRKNAEGGLV"
     gene            1211..2014
                     /locus_tag="PLI69_06140"
     CDS             1211..2014
                     /locus_tag="PLI69_06140"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG42099.1"
                     /translation="MKKFFAKAYIWFLLVVLYAPIIFIMIFSFSESRVLGNWTGFSTN
                     LYANLFSPQAASATSLFKAIYNTLIIALISAFVSMILGAIAAIGIYNLRSRNKNIMLF
                     LNSIPIINPDIITGVALFLLFIFLHFSQGYTTVILAHITFCTPYVVLSVMPRLTQMNP
                     NLYEAALDLGATPIQALRKVLVPLLRPGMISGFILAFTMSLDDFVVTFFTRGTIGLET
                     LSTYIYADARKGGLTPELRPLMTIIFLTILILLVIFNVRKVKSEKQLAK"
     gene            2011..>2544
                     /locus_tag="PLI69_06145"
     CDS             2011..>2544
                     /locus_tag="PLI69_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG42100.1"
                     /translation="MKRLFTLLAALLLIVSCTSDRSKYLKVYNWGDYIDESLIGEFEQ
                     WYYEQTGEEVKIIYQTFDINETMLSKIEKGEEDFDVVCPSDYIIERMLREDMLLPIDR
                     DFGDTHNYIDDNIAPYIRECFDKIDGSGKNANDYSVGYMWGTAGILYNKKHVAEEDLG
                     TWDIIRNAKYANKIFIKD"
BASE COUNT          671 a          559 c          575 g          739 t
ORIGIN      
        1 ataccggtta tgaattgatg gtacaggact ataacgcttt tgaagtgggt tctacagttg
       61 gtcttgtcat aagacctgag gatatccagg ttatgcataa ggagcgctca aggaatactt
      121 ttgaaggaga gatggtggat gaaaaccatg tacgtttcct tgatggtgaa tttgaatgtc
      181 ctatccagcc gggagttgaa aaggggcaga aggtgaaagt agaggtggaa tttgataagg
      241 ttgatctcta tgaccaccag gaagagggaa ctctttccgg ctctgtgcat tttatgatct
      301 ataaaggaaa tcactaccat atcacggtgt taaccgatga aggtcatcat atctgggtag
      361 acaccaatga cgtatgggac aaaggagacc tggtaggcat taatttccct gctaaacaca
      421 tcaagataac ccagttgcaa tgaataaacg aagcagttgg agtctgccat atctgatctt
      481 tctggtcatt ttcgtggtgc ttcccctgat tttggtgctg gtatatgcct tccaggatgc
      541 tcagggccga ttttcgtttg aaaacatcac gaggtttttc tctgataacg atgctttaag
      601 caccttcggt ctatcgctgg agattgccat tttaaatacc ctgatctgcc ttctgctggg
      661 ttatcctgct gcctggatcc ttgccaacaa gaaatacaat cgcagtgcag tcactgtggt
      721 gctttttatc ttaccaatgt ggataaatgc cctaatgagg actctggcga ccgccgagct
      781 ttttaacgtg ttgggtatca cattgggcaa aggaactctg ctcttcggta tgttttatga
      841 ctaccttcca tttatgatct atcctatata caatattctc agcaggatag accattccta
      901 tgcagaagct gccgaggacc tgggggctac cccatgggag gtattttgga aggtgaccgt
      961 gccactttca attccgggag taatcagcgg agtgatgatg gtattcatgc ctacggtttc
     1021 cacctttgcc atctccgaat tcctgaccaa caacaagatt aagctctttg gtaccattat
     1081 tcaggagaat atcaactcct caatgtggaa ttacggagcc gccctcgcct tgataatgct
     1141 ggtgataatc ggtatcacta cattcttttc aaatgatcgc aaaaatgccg aaggagggct
     1201 tgtatagggg atgaagaagt tttttgccaa agcatatatc tggtttctgc tggtcgttct
     1261 ctatgctccc atcattttca taatgatatt ttccttctcg gaatcaaggg tgttgggaaa
     1321 ctggacggga ttttccacaa atctctatgc aaatctcttt tcgccgcagg cagccagtgc
     1381 tacatcactc ttcaaggcta tctacaatac tttgataata gccctgatat cggctttcgt
     1441 atcgatgatt ttgggtgcta tcgccgccat aggcatttat aacctgcgca gccgcaataa
     1501 gaacattatg ctatttctca acagtattcc tataatcaat ccggatatta ttaccggtgt
     1561 agcgctcttt ttgctcttta tattcttgca tttttcgcag ggttatacca ccgtgatatt
     1621 ggcacacatc accttctgta ccccttatgt ggtgctgagc gtgatgcctc gactaactca
     1681 gatgaatcca aacctctatg aggcggcgct cgatctgggg gctactccca tacaggcgct
     1741 acgcaaggta cttgtacctc tcttacgccc cggaatgatc tccggattca tacttgcatt
     1801 tacgatgtcg ctcgacgact ttgtagttac attctttacc agaggaacga taggacttga
     1861 gacactctct acctacatct acgcagatgc gcgcaagggc ggtctgactc ctgaattgcg
     1921 tcctctaatg accataattt tcttaactat ccttatcctt ctggtgatat tcaatgtcag
     1981 gaaggttaaa tctgaaaaac aactagcaaa atgaaaagac tttttaccct tctggcagct
     2041 ttgctgctca ttgtatcctg cacaagtgac aggagcaagt atctgaaagt ttacaactgg
     2101 ggcgactata tagacgagtc ccttatcggc gagttcgagc agtggtatta cgaacagacc
     2161 ggtgaagagg tgaaaatcat ctatcaaacc ttcgatatca atgagactat gctctcaaag
     2221 atcgaaaagg gagaggagga cttcgacgtg gtatgtccct cggattatat catcgagcgc
     2281 atgctccgcg aggatatgct tctgcccatt gacagggatt tcggcgacac tcacaactat
     2341 atcgatgaca atattgcgcc ctatatccgc gagtgctttg ataagattga cggcagcggc
     2401 aaaaacgcca atgactactc tgtgggttat atgtggggaa ctgctggtat tctttataac
     2461 aaaaaacatg tggcggaaga agatctgggt acctgggata ttatccggaa cgccaaatat
     2521 gccaataaaa tcttcataaa agat
//
LOCUS       DAOSIE010000218         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_35465_length_2540_cov_3.571429, whole genome shotgun sequence.
ACCESSION   DAOSIE010000218 DAOSIE010000000
VERSION     DAOSIE010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35465_length_2540_cov_3.571429"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1787)
                     /locus_tag="PLI69_06150"
     CDS             complement(93..1787)
                     /locus_tag="PLI69_06150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG42101.1"
                     /translation="MRRILSLLLLFCLFVLNAQEIPISPLNLPISLAGSYGELRRDHF
                     HTGLDWRVGGVVGDSIYAIKSGYISRLSVSVGGYGNAVYITHPDGTLSVYGHMHDFVP
                     AYAKLVETEQYASERFEVSLEFEQGELPVLQGEAIGRVGNTGFSVAPHLHMEVRDLAT
                     QTPLNYLARGYYKVKDDIAPQFQRLFFFGYCDTLTLPLRYPLRSSNSPTSLSGTTVKL
                     PHKSYLGIDAIDRQNGTTGKLAVMEYLVTLDKDTIFRFEVGEIHPRQGRYLNALRETT
                     IQGDDVIKTLVDPNNLLSDHIYAPGGGLLVLNDYQEHDLRVRISDEHGNRSTLTFKVR
                     RDDSLETSLSAVAAAIESDEDVQRIAIPWYLPHLFIDDGVTYTLPAASLYDSGWFPYA
                     KVADADSSSNIFSAVWKLGDPSLPMHRRGEIFLESSLPDSLRPKACLAHYNDGILSYA
                     GGKWEGNGVRGSVGFGTWCVTTDTTPPKLSFWERKGNILRAGGEILIYVEDDFSGIKD
                     HRVEIDGKWHLSMLKRGRISLRLDPSRVPRGSKHTIVVRAEDNCGNSTIISRDFFF"
     gene            complement(1789..>2540)
                     /locus_tag="PLI69_06155"
     CDS             complement(1789..>2540)
                     /locus_tag="PLI69_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOG42102.1"
                     /translation="DVVKGVDKKIKINKMVQCPECKGKGALSEADIKSCEHCDGRGVV
                     TRVVQTMLGQMQTQSTCSHCKGEGKRVVNPCRKCNGSGLVKKAEEISFSIPPGVMDGM
                     QLTIKGKGNASKAGGVNGDLLVVIEEEEHPELKRDDNDLLYNLYISTIDAMLGTDVEV
                     PSIDGKLRIKIEPGTQPGKILRLKGKGVPDINRYGTGDMIVFVQVWIPKKLDKKDKEI
                     LEKFRNSEAFKPDPDQYDRQFFDKVKNFFNR"
BASE COUNT          692 a          612 c          567 g          669 t
ORIGIN      
        1 cgcgaagggt tgtatagaaa ataacggtga gatatatagg ttattagcaa acaacggggt
       61 gatttcatca ctccgttgtt gttttatccc ggttagaaga aaaagtctcg ggaaattata
      121 gtgctgtttc cgcaattgtc ctcagccctg accacaatag tatgcttact gcctcgcgga
      181 actcgtgagg gatctagacg gagggagata cggccacgtt ttaacatcga aaggtgccat
      241 ttgccatcaa tttccacccg gtgatcctta attccggaaa aatcatcttc aacatagata
      301 agaatctcac ctcccgcacg taggatgttg ccttttcgtt cccaaaacga gagtttggga
      361 ggtgtggtat cagttgtaac acaccaggta ccgaaaccta cactgccacg tactccatta
      421 ccttcccatt tgccacctgc ataagaaaga ataccatcat tataatgtgc aaggcaggcc
      481 tttgggcgga gggagtccgg aagagaagac tcaaggaata tttcgcccct gcgatgcatg
      541 ggaagcgagg gatctcccag tttccagacg gcagagaaaa tatttgaaga tgagtcggca
      601 tcagccactt tggcataggg gaaccatccg gagtcataaa gtgatgccgc gggcagagta
      661 taggtaacac catcatctat aaatagatgg ggcaaatacc aaggtatagc tatgcgctga
      721 acatcttcat cactttcaat ggctgccgct acggctgaga gggaagtttc aagagagtca
      781 tcacgcctta ccttaaaagt gagagtggag cgattgccgt gctcatcact gatacgaacc
      841 ctgagatcgt gctcctgata gtcattgaga accaacagac cacctccggg ggcatatata
      901 tgatcagata gaagattatt ggggtctaca agagtcttga tgacatcatc gccttgtatg
      961 gtggtctcgc gcagggcatt gagataacgc ccctgtctcg ggtggatctc acctacttca
     1021 aatctgaaaa tggtgtcctt atcaagcgtg accagatatt ccatgactgc cagcttaccg
     1081 gtagtaccat tctgtcgatc gattgcatct atgccgagat agctcttatg gggcaatttg
     1141 actgtagttc cggagagaga agtgggagaa ttggagcttc tcagtggata tcttaaagga
     1201 agtgtcagcg tgtcacaata accgaagaaa aagagacgct gaaactgtgg agctatatca
     1261 tcttttacct tataatagcc cctggccaaa taattgagcg gggtctgagt agcaagatca
     1321 cgcacttcca tatgaagatg tggtgcaacg gaaaaaccgg tattccccac tctacctatg
     1381 gcttctcctt gtaataccgg caactctccc tgctcaaatt caagcgagac ttcaaatcgt
     1441 tcggaagcat actgttccgt ctctaccagc ttcgcgtaag cgggaacaaa gtcatgcatg
     1501 tggccgtaaa cggagagcgt accatccgga tgcgttatat agactgcatt accataccct
     1561 ccaacagaaa cactgagacg agagatatag cccgatttta tggcatatat gctatctcct
     1621 actacacctc cgacacgcca gtccaaaccg gtgtgaaagt ggtcgcgcct caactcccca
     1681 taagaacctg caagagaaat cggcagattt agcggagaaa taggaatctc ctgtgcgtta
     1741 agcacaaaca agcagaacag caacagaaga gagagaattc tacgcatgtt atctattgaa
     1801 aaagtttttg actttatcaa agaattgtcg gtcgtattga tcgggatcgg gtttgaatgc
     1861 ttccgaattt ctgaatttct ccaatatctc tttatccttt ttgtcgagtt tcttaggaat
     1921 ccatacctgt acgaacacaa tcatatcacc cgtaccatag cgatttatat ccggaactcc
     1981 ttttcccttg agacgcaata tcttacctgg ctgagtgcct ggttcgattt ttatcctaag
     2041 tttgccgtca atggaaggta cctctacatc ggttccgagc attgcgtcaa tcgtcgaaat
     2101 gtagagattg tagagcagat cattatcatc acgtttgagt tcaggatgtt cttcttcctc
     2161 aataaccaca aggaggtctc cattgacccc accagcttta gatgcgtttc ctttaccctt
     2221 gatggtaagc tgcatgccat ccataactcc cggaggaatc gagaatgata tttcttcagc
     2281 ctttttaaca agacctgaac cattacattt acggcaggga ttaaccacac gctttccctc
     2341 tcccttacaa tgagagcagg tagactgtgt ctgcatctga cccagcatag tctggaccac
     2401 cctggtcaca actcccctgc catcgcaatg ctcgcaactc ttgatgtcgg cctccgagag
     2461 cgctcctttg cccttacatt cgggacattg gaccattttg ttgatcttga tttttttatc
     2521 aacccccttc actacatcgg
//
LOCUS       DAOSIE010000219         2535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_35552_length_2535_cov_3.684677, whole genome shotgun sequence.
ACCESSION   DAOSIE010000219 DAOSIE010000000
VERSION     DAOSIE010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35552_length_2535_cov_3.684677"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLI69_06160"
     CDS             <1..205
                     /locus_tag="PLI69_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42103.1"
                     /translation="DVLLINTHISIAHSNLEDLMLLVALGAIGWVMLLIRWAMAIVLV
                     WELKLEFYIKDAFAKRKNRNFAV"
     gene            227..1159
                     /gene="trxB"
                     /locus_tag="PLI69_06165"
     CDS             227..1159
                     /gene="trxB"
                     /locus_tag="PLI69_06165"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOG42104.1"
                     /translation="MNNEKIKCLIIGSGPAGYTAAIYASRAALHPVLYEGMQPGGQLT
                     TTTEVENFPGYPEGVLGQQMMADLRAQAERFGTDIRYGMVTEADLSKRPFKITIDEEQ
                     VIEAETLIIATGATARYLGLPSESKYRGMGVSACATCDGFFYRGKTVAVVGGGDTACE
                     EALYLASICKKVYMVVRRDVFRASKAMQDRIASTENIEVLWNSNTLEVLGDEMGVNGM
                     RLIRKDGEVYEKAIDGFFLAIGHHPNSDLFKEWVNTNEEGYIITDGNSTKTNVPGVFA
                     AGDVQDPHYRQAITAAASGCKAALDVEKFILENK"
     gene            1161..1985
                     /gene="bamD"
                     /locus_tag="PLI69_06170"
     CDS             1161..1985
                     /gene="bamD"
                     /locus_tag="PLI69_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOG42105.1"
                     /translation="MIKKYRLILALAFGAVIGLSACKSQYDTILRSNDVPAKYALAFE
                     MFEAKKYLKAAELFESLTIATNGTPQDDTVQYYWGLSNYYYGDFTTAESNFNQFISIY
                     PLSPFTEKAKFYRLDCLYRGTYRYELDQRPTYKALSAIHEFVVENPKSEYIPRCHEMA
                     DELNGRLELKSYKSAYLYYHMEDYLAAHFALKNVLKENADNRYREEVLYYTAMSAYKY
                     ASMSVPEKQRERYLTFVDDYFNVISEYPESKYRKELDGLYSRVQTILKKNSPDENN"
     gene            1999..2313
                     /locus_tag="PLI69_06175"
     CDS             1999..2313
                     /locus_tag="PLI69_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOG42106.1"
                     /translation="MSNTKPISNNTETRNLSELADPTGNIYESVKIISIRSNQIAASV
                     KKELSEKLAQFKSDADALEETFENKEQAEISKYYEAQPKPTLVAINEFEENRIYYRKA
                     EE"
     gene            2342..>2535
                     /locus_tag="PLI69_06180"
     CDS             2342..>2535
                     /locus_tag="PLI69_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG42107.1"
                     /translation="MLSRLTIQNYALIESLDITFPDNLVIITGETGAGKSILLGALSL
                     VLGGRSDISTLKDRTRNCVV"
BASE COUNT          752 a          544 c          581 g          658 t
ORIGIN      
        1 tgatgtattg ctaattaata cccatattag catagcgcac agcaatctcg aggatcttat
       61 gttacttgtc gctttgggag cgataggatg ggtgatgtta ctgataaggt gggccatggc
      121 catcgttttg gtatgggaat tgaaattgga attttatatc aaagatgcct ttgccaaaag
      181 aaaaaatcgt aactttgcag tttgaattaa ttgagataat gtttttatga ataacgaaaa
      241 aataaaatgt cttataatag gtagcggccc cgcagggtat actgctgcaa tttatgcttc
      301 tagagctgct ttacatccgg ttttgtatga agggatgcag cccggtggtc aacttaccac
      361 cactactgaa gttgaaaatt tccccggtta tcctgaaggt gtattaggac agcagatgat
      421 ggcggacctg cgcgctcagg ccgaacgttt tggtacggat atcagatatg gcatggttac
      481 agaagcagac ctatccaaaa ggcccttcaa gataaccatt gatgaagaac aagttataga
      541 ggcagagact ttgattatcg ccaccggtgc tactgccaga tatctcggtc tgccttcaga
      601 aagcaagtac agggggatgg gagtttcggc ctgcgctact tgcgatggtt ttttctatcg
      661 cggaaagacg gtagcagttg taggaggcgg ggatacggcc tgtgaagagg cgctttacct
      721 cgcttctatt tgcaaaaagg tatatatggt agtgagaaga gatgttttca gggcatccaa
      781 agccatgcag gataggattg cttccactga gaatattgaa gtactttgga atagcaatac
      841 tctggaagta cttggagatg aaatgggtgt aaacggcatg cgccttatcc gcaaggatgg
      901 cgaagtgtat gaaaaggcca tcgacggctt cttcctggct atcggtcatc accccaattc
      961 ggatctcttc aaagagtggg tcaacaccaa cgaagagggt tatataatta ccgacggaaa
     1021 ttccaccaaa accaatgttc cgggagtttt tgcagccgga gatgttcaag acccccacta
     1081 tcgccaggcg attacagcag cagccagtgg ttgcaaggct gccctcgatg ttgagaaatt
     1141 tattttggaa aataaataat atgataaaaa agtacagact tatcctggct ctcgcttttg
     1201 gggctgttat tggtcttagc gcatgcaagt cgcagtacga caccattctt cgcagtaatg
     1261 acgttcctgc caaatatgcg ctggcattcg aaatgtttga agccaagaaa tatctcaagg
     1321 cagcggaact cttcgaatcg ctgacaattg ccacaaatgg cacgcctcag gatgatactg
     1381 tacaatatta ctggggcttg agtaactact attatggtga ttttacaact gccgagagca
     1441 attttaatca gttcataagc atctatcccc taagtccttt tacggagaaa gccaagtttt
     1501 accgcctgga ctgtctttac agaggcactt accgttatga acttgaccag cgtccgacat
     1561 acaaagccct tagcgccatc catgaatttg tggtagagaa tcctaagtcg gagtatattc
     1621 ccagatgtca tgagatggca gacgaactca acggtcgtct ggaactcaag tcttataagt
     1681 cggcctacct ctactatcat atggaggatt atcttgcagc ccattttgct ttgaaaaatg
     1741 tcctcaagga gaatgctgat aaccgttatc gcgaagaggt actttactat acggcaatgt
     1801 cagcatacaa atacgcttct atgagtgtgc cggaaaaaca gcgtgaacgc taccttacat
     1861 tcgtggacga ctatttcaat gttataagtg aatatcccga atcaaagtac cgaaaggaac
     1921 tcgacggact ctattccagg gtccaaacaa ttcttaaaaa gaattctccc gacgaaaata
     1981 attaataata attccataat gagcaacact aaacctataa gcaacaacac ggaaacacgc
     2041 aatctttccg agctcgctga tcctacaggc aatatatacg agtccgtaaa gatcatctcc
     2101 atcaggtcca accaaattgc tgcttcagta aaaaaggaac ttagcgaaaa gctcgcacag
     2161 ttcaagagtg atgcagatgc tctcgaagag acctttgaaa acaaagagca ggcggaaatc
     2221 tccaagtatt acgaagctca gcctaaaccc actctcgtag ctataaacga gtttgaggaa
     2281 aaccggatct attaccgcaa ggcagaagaa taataccact gccctcgcat agagtatccg
     2341 tatgctcagt agattgacaa tacagaacta tgccctgatt gagagtctgg atatcacttt
     2401 tccggacaat cttgtcatca tcaccggtga aaccggggcg ggcaagtcaa ttctacttgg
     2461 ggccctatca ctcgtgcttg gtggacgctc ggatatctct acacttaaag accgtactcg
     2521 caattgcgtg gtaga
//
LOCUS       DAOSIE010000220         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_35722_length_2526_cov_3.240793, whole genome shotgun sequence.
ACCESSION   DAOSIE010000220 DAOSIE010000000
VERSION     DAOSIE010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35722_length_2526_cov_3.240793"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..992)
                     /locus_tag="PLI69_06185"
     CDS             complement(42..992)
                     /locus_tag="PLI69_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG42108.1"
                     /translation="MDLSIVISLFNEEESLPELVAWISRALASEQLKYEIIMVDDGST
                     DNSWQVIEELKKSYPAIRGISFRRNYGKSAALYTGFEAATGDIVVTMDADLQDSPEEI
                     PEMMRMIKEEGYDLVSGWKKKRYDGTFTKNLPSKLYNATARKVTDTKLHDMNCGLKAY
                     RNEVVKNIEVYGEMHRFIPYLAKQAGFAKIGEKVVQHQARKYGKSKFGLNRFINGFLD
                     LLSVWFLQVFGKKPMHFFGSIGVLTFVIGIVIAIWLIVAKLIAQSRGIIFRPVTEQPL
                     FYLALLMALIGVTLFLTGFLGELITRNSQERNKYNIRKEI"
     gene            complement(996..1520)
                     /locus_tag="PLI69_06190"
     CDS             complement(996..1520)
                     /locus_tag="PLI69_06190"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 family protein"
                     /protein_id="HOG42109.1"
                     /translation="MALNTTTISNWREAARDGLLLALVTVVLISFNALVENAALKMLI
                     WLVKFVGSVWLLHFFMKKFMKGEPRNYVFRQGVRITLFSSIICAAYTFFMYSVLFPEM
                     ITEIFEAFYSAPEFASLPDNMTDMMLKLEDNFARYSCIATFFWNFILGIIISAIIARS
                     IVSKTIFTEDDNTI"
     gene            1625..>2526
                     /locus_tag="PLI69_06195"
     CDS             1625..>2526
                     /locus_tag="PLI69_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOG42110.1"
                     /translation="MIKIKFPDGTVKEFPKGTTPYQIAESISPRLAAEILVATVNDSI
                     HDLDRPIHEDATIKLHKWEDQEAKSTFWHSSSHLMAEALEILYPGVKFGIGPAIENGF
                     YYDVDLGERQIVEADLAKIEKKMIELAQQKEHFTRIDVSKAEAMEKYSAKGDEYKCEL
                     INDLEDGTITFYTNGVFTDLCRGPHLRDTSVIKAVKLTTIAGAYWRGNEKNKMLTRIY
                     GITFPKKSLLDEYLKMLEEAKKRDHRKLGKELELFAFSSKVGQGLPLWLPRGAALREQ
                     LEMFLKKVQKAYGYLPVITPHIGQ"
BASE COUNT          774 a          559 c          531 g          662 t
ORIGIN      
        1 ctccggagag cgagaatagt aagaataata caaaatagat tttaaatctc cttacggata
       61 ttgtatttgt tcctctcctg gctgtttctt gtaataagtt caccgagaaa tcctgttagg
      121 aaaagtgtta ctcctatcaa ggccatcagc agtgccagat aaaatagggg ctgctccgta
      181 acagggcgga atataatacc gcgcgactga gcaatgagtt tagccacaat cagccagata
      241 gcgataacaa tacctattac gaaggtaagt acaccgatgc tcccaaaaaa gtgcatcggt
      301 ttttttccga atacctgaag aaaccacacg gaaagcaggt ccagaaaacc attaatgaag
      361 cggtttaaac caaacttgct tttaccatat ttacgagcct ggtgttgtac caccttctca
      421 ccgattttgg caaaacctgc ctgcttagcc agatagggaa taaaacggtg catctcccca
      481 tagacctcga tattcttcac aacctcattg cgataggcct tgaggccaca gttcatgtcg
      541 tggagtttgg tgtccgttac ttttcgagcg gtagcattat agagttttga agggagattt
      601 tttgtaaaag tgccgtcgta acgttttttc ttccatcctg agacaagatc atagccctct
      661 tcctttatca tcctcatcat ttccggaatt tcttccgggc tatcctggag gtctgcatcc
      721 atagtaacta ctatgtcccc tgttgctgct tcaaatcccg tataaagagc tgcagacttg
      781 ccgtagttgc ggcggaatga gatacctctg attgcagggt agctcttttt aagctcctca
      841 atgacctgcc atgagttatc ggtactccca tcatctacca ttatgatttc atatttcaat
      901 tgctcggaag cgagtgctct gcttatccag gctaccagct ccggaaggga ttcttcttca
      961 ttgaaaagtg atattactat agaaagatcc atatctcaaa ttgtattgtc gtcctcggta
     1021 aaaatggttt tagaaacaat ggaacgcgca attattgctg aaatgattat acctaagatg
     1081 aaattccaga aaaaagtagc gatgcaggaa tacctcgcaa agttgtcctc cagcttgagc
     1141 atcatatctg tcatattgtc cggaagcgaa gcaaattccg gtgcagagta aaacgcctcg
     1201 aatatttccg taatcatctc gggaaaaagt acgctataca tgaagaaggt gtatgctgca
     1261 cagataattg aagaaaagag agtaattctt actccctgcc tgaaaacgta attcctcggc
     1321 tcacccttca tgaatttttt cataaaaaaa tgaagtaacc atactgagcc cacaaatttt
     1381 accagccaaa tgagcatctt aagtgctgca ttctcgacaa gtgcgttgaa cgaaatcaaa
     1441 accaccgtaa ccagagcaag taatagaccg tcgcgagccg cttctctcca atttgaaata
     1501 gttgttgtgt taagtgccat gtatctgtta ttcaatataa ataattgctg ttaagattac
     1561 aaagataaga aataaagtct tacctttgta ggttgaaata aaaaattgaa caataatata
     1621 gaccatgatt aagataaaat ttcctgacgg cactgtaaag gagtttccaa agggtaccac
     1681 gccttatcag atagcagaga gtatcagccc aaggcttgca gccgaaatac ttgttgctac
     1741 tgtcaacgat tcaatacacg accttgacag gcccattcat gaagatgcca ccattaaatt
     1801 acacaaatgg gaggaccagg aggcaaaaag tacattctgg cactcctcca gccaccttat
     1861 ggcggaggct ctcgaaatac tctatccggg agtcaaattc ggtataggcc ccgctatcga
     1921 gaatggcttc tactacgacg tggaccttgg ggagagacaa atagtggagg cagaccttgc
     1981 caaaatagag aaaaagatga tcgagcttgc tcagcagaaa gaacacttca ccagaataga
     2041 tgtctctaaa gctgaagcga tggaaaaata tagcgctaaa ggagatgagt acaaatgcga
     2101 actcattaat gacctggaag atggcaccat aactttctat accaacggtg tttttacgga
     2161 tctttgtagg ggccctcacc ttagagatac ctccgtaata aaggcggtta aactcaccac
     2221 aattgcgggt gcctattgga gaggtaatga gaagaacaag atgctaaccc gcatctacgg
     2281 tataactttc cccaagaaat ctctcctcga cgaatacctc aaaatgctcg aagaggccaa
     2341 aaagagagat caccgcaaac ttgggaaaga gcttgaactc tttgcatttt cctcgaaagt
     2401 gggccagggt ctgccgttgt ggctcccccg gggtgctgct ttaagggagc aactcgagat
     2461 gtttctcaaa aaggtacaga aggcatacgg ttatttgccg gttattaccc ctcacatcgg
     2521 ccagaa
//
LOCUS       DAOSIE010000221         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_35894_length_2517_cov_2.727457, whole genome shotgun sequence.
ACCESSION   DAOSIE010000221 DAOSIE010000000
VERSION     DAOSIE010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35894_length_2517_cov_2.727457"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /gene="priA"
                     /locus_tag="PLI69_06200"
     CDS             <1..897
                     /gene="priA"
                     /locus_tag="PLI69_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOG42111.1"
                     /translation="APYAQCTECGTVVKCPSCDVALSYHKFDNMLKCHYCAYQIPFEP
                     TCPRCASPSIVDRGAGTEKIEELLREYFPDYVIERFDSDTILGKSEEQRILRSFAEGK
                     IDVLVGTQMITKGFDFDRLSLVAVVGCDSLFSVQDFRADERALQLLWQLRGRAGRRED
                     GARMIIQTEQKEHPVLKALIGESDQDDLSAAFEERKRFGYPPFVRLVGITLKDKNEKR
                     MWSCAYDLSDAIRGIGIREFNGPVVPPIAKMGNYYQVQFQLRLRRNSSLSRIKKALYF
                     ETELLNRKYNGMTSINIDVDPY"
     gene            complement(908..1666)
                     /locus_tag="PLI69_06205"
     CDS             complement(908..1666)
                     /locus_tag="PLI69_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOG42112.1"
                     /translation="MVDQMVGTQVIAHRGYWKQEGSAQNSISSLRNAHRVGVYGSEFD
                     VQITADSILIVNHDNDFKGHIINLTNYKVIKDSLLSNGEKLPTLEDYLAAGKKLKGLQ
                     LVLELKPVKDNPELEKYAVEKCVEMVKKIGLEKRVDYISFSLEACKGFAALMPKASVA
                     YLGGYYMPIELLEMGINGFDYHYNEVLANRFWVDEARNYKMTSNVWTVNDSMLVETLL
                     VGNPTFLTTDNPEEVTRQIEALRERVRERLSGKQ"
     gene            1865..2305
                     /locus_tag="PLI69_06210"
     CDS             1865..2305
                     /locus_tag="PLI69_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOG42113.1"
                     /translation="MLDNSITTEIKKILTRYGLKATPQRIVVYFAMRELGHASADMVY
                     QRASSELATLTVATVYNVLESFVEVGLLQRRMSSNNKMYMDVNTYNHCHLYCEETHTY
                     KDYDDPELMLMIHNHFRNKKIRGFDLYRIDVQLVGRNSQSNRAQ"
     gene            2360..>2517
                     /locus_tag="PLI69_06215"
     CDS             2360..>2517
                     /locus_tag="PLI69_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG42114.1"
                     /translation="MKSLKGTQTEKNLLQSFAGESQARSRYVFFASRAKKEGYEQIAA
                     IFAETAEQ"
BASE COUNT          718 a          513 c          554 g          732 t
ORIGIN      
        1 gccccttatg cccaatgtac ggagtgtggg acggtagtca aatgtccctc gtgtgatgtg
       61 gctctaagct accataagtt tgataatatg ctcaaatgcc actactgcgc atatcagata
      121 ccttttgaac ccacatgccc ccggtgtgcc tcgccctcta ttgtcgatcg tggtgccggt
      181 accgagaaga ttgaagagtt gctgcgcgaa tacttcccgg attatgttat cgaaaggttt
      241 gattcagata ctattttggg caaaagcgaa gagcagcgta tcctcagaag cttcgctgaa
      301 ggaaaaatag atgttttggt aggaactcag atgattacca aaggctttga cttcgaccgc
      361 ctatctctgg tagcggtcgt aggctgcgac tctctttttt ccgtacagga tttcagagcg
      421 gatgaacgcg ccctccaact cctatggcag ttgcgtggta gggcaggtcg cagagaggat
      481 ggggccagga tgataattca gactgagcag aaggagcatc cggtattgaa agccctaatt
      541 ggtgagagtg atcaggacga tctttctgct gcctttgagg aacggaaaag gtttggatac
      601 cctccatttg tgcgtttagt ggggattacc ctgaaggata aaaatgaaaa gaggatgtgg
      661 agttgcgctt atgatttgag tgatgcaatc agaggcatcg gtattagaga attcaatggt
      721 ccggtggtgc cgccgatagc gaaaatgggc aactattatc aggttcagtt ccaactgcga
      781 cttaggagaa atagttctct ctctcgtata aaaaaagccc tgtacttcga aacagagctc
      841 ttgaaccgta aatataacgg gatgacttcc atcaatattg acgtggatcc ctattagtga
      901 taacactcta ttgttttccc gagagtcgct cccttaccct ttctcttagg gcctcaattt
      961 ggcgtgttac ctcttccgga ttgtcggtgg ttaggaaagt agggtttcct acgagaagag
     1021 tctctacgag catcgaatcg ttgaccgtcc agacattgga agtcattttg taattgcgtg
     1081 cttcatccac ccagaaacga ttggcaagga cttcgttata gtggtagtca aatccgttga
     1141 ttcccatttc cagcagttct atcggcatat aatatccgcc taagtaggca acggatgcct
     1201 ttggcatcag agctgcgaat cctttacaag cctcaaggct gaacgagata taatccaccc
     1261 gtttttccag tccaattttt tttaccattt caacgcattt ctcaactgca tacttctcaa
     1321 gttccggatt atctttgacc ggttttagtt cgagtacgag ctggagtccc tttagttttt
     1381 tacctgcagc cagataatcc tcaagtgtag gaagtttttc cccgttggaa agtagggagt
     1441 ccttgattac tttatagttt gtaaggttga ttatgtgtcc tttaaagtca ttgtcatgat
     1501 tgacaatcag tatactgtct gcggtaattt gtacgtcaaa ctctgaaccg tataccccca
     1561 ctctatgtgc attacgtaga gaagaaattg aattctgtgc actaccttcc tgtttccagt
     1621 aacctctatg agcaattact tgtgtgccga ccatttggtc taccattttt tcaattctat
     1681 tctctttaca tcctgagatt gtaaacagta gcattaaagc gactaaagac catgaaaatt
     1741 tcataataaa taatctttgt actattattg tatgtgcaaa tttattaaaa aaaagaattt
     1801 tgtataataa actttgtgtt tttgaataat tctaattaac ttcgccgcgg taaactcaac
     1861 aaaaatgcta gataactcaa taaccacaga aataaagaaa atcctaacga ggtatggact
     1921 aaaagccacc cctcaacgaa ttgtcgtata ttttgcgatg agagagttgg ggcatgcatc
     1981 agctgatatg gtctatcagc gggcaagttc agaattagcc acattgactg ttgccactgt
     2041 gtacaatgtt ctggaatcct tcgttgaggt tggactacta cagcgtcgta tgagttccaa
     2101 taataagatg tatatggatg tcaataccta caaccattgt catctctatt gcgaagagac
     2161 tcacacctat aaggactatg atgaccccga gttgatgcta atgatacata atcattttcg
     2221 taataagaaa atacgaggat ttgacctgta tcgaattgat gtgcagctgg tgggtagaaa
     2281 cagtcaatct aacagagcac aataatgttg gtactaaacg gtactatcaa tcaaaccaac
     2341 cttttttaaa ataaacaaaa tgaaatcatt aaaaggaaca cagacagaaa agaacttgct
     2401 acaatcattt gcaggtgaat cccaggcgcg cagtagatac gttttctttg cgagcagagc
     2461 taagaaagag ggctatgagc agattgcagc tatttttgca gagacggcag agcaaga
//
LOCUS       DAOSIE010000222         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_35987_length_2511_cov_3.010179, whole genome shotgun sequence.
ACCESSION   DAOSIE010000222 DAOSIE010000000
VERSION     DAOSIE010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35987_length_2511_cov_3.010179"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PLI69_06220"
     CDS             complement(<1..967)
                     /locus_tag="PLI69_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOG42115.1"
                     /translation="MKDFLKVLLASFVGTILAFLIFGFIGIGMLVGLASLGSAEEPII
                     AKESILLLDFKAPVTEQKGDKFAINPITGDIDMSGSIPLYSVVKAIEVAAEDPGIKFI
                     YMTPESLNMGISQIEEVRDALKKFRSSGKAVVSYSTDLGNDSYYLASVADKVILNPYG
                     DIFILGLSSNMIFFKDLLDRLGVDVQLIKHGKYKAAAEPFVQNEISAENLEQNKRMLG
                     SIWNGWCEEIAESRDFTSEQFNFWIDNLEFKGATSALEKGLVDELWYSDQVVEYLCSL
                     FGVTESKDLKFTPIGKYSAARVKTDLRAKEKIAVVYANGEIVLEGS"
     gene            1038..1478
                     /locus_tag="PLI69_06225"
     CDS             1038..1478
                     /locus_tag="PLI69_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42116.1"
                     /translation="MKKLAKYLFSSLLTILYITGFVGFGVHKCHTEGTADLFLMMGDI
                     SCESIHHHIHDHNDCCDAQCDSHPFHYTDCSSECCSTDVFAVTSEQDRLQDEDMGLSA
                     QTIAVSLLCDDLTALSYSTIVSPSGDLNPLKVGLPLSALSIWRL"
     gene            1597..>2511
                     /locus_tag="PLI69_06230"
     CDS             1597..>2511
                     /locus_tag="PLI69_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG42117.1"
                     /translation="MRKAIFSALFILFCTTILAQDFKGSIFYRRSDGKTEPLPFAQVY
                     LIEKARLIETDENGQFTINLEVRTTMVATYVGYTKDTLIVDPGIPSGEFYLMGENEVD
                     EAVVTSRQLTNTLSRIKPVRTEVITAAGLCKMACCNLAESFENSASVTVGYSDAITGA
                     RQIKLLGLAGTYTQLLDENRPVMRGLASPFGLTYVPGQWLESIQIAKGPSSVINGLEA
                     ITGQINMEHRKPTDEVPFFLQLFGSSHAMAEGNIVSSLQLNEKWSTVILAHASSLLVG
                     SDHNDDGFRDEPQATQFNLSNRWLYYDPS"
BASE COUNT          691 a          598 c          525 g          697 t
ORIGIN      
        1 cacttccttc cagcacaatt tctccattag catatactac cgcaatcttc tccttggctc
       61 taagatcagt tttaacacgc gctgcggaat atttaccgat gggggtaaat ttgagatctt
      121 tcgattcggt aactccaaag agagagcaca gatactctac aacctgatcg ctgtaccaca
      181 attcatccac aagacccttt tcaagtgcac tagtggcgcc tttgaactca agattgtcaa
      241 tccaaaagtt gaattgctcc gaggtgaaat cccgggactc cgcaatctct tcacaccagc
      301 cgttccagat agagccaagc attcgcttgt tttgttcaag attttcagca ctgatttcat
      361 tttggacaaa gggctcggcc gcagctttat atttaccatg ttttataagc tgcacatcca
      421 caccgaggcg atccagaagg tctttgaaaa atatcatatt cgaactcaat cccagaatga
      481 atatatctcc atagggattt aggatcactt tgtctgcaac agaagcgagg tagtagctat
      541 cattccccaa atcggtggag taggagacta ctgctttacc tgatgaacgg aattttttga
      601 gggcatccct gacctcttct atctgagaaa ttcccatatt gaggctctcg ggagtcatgt
      661 agataaactt tatgccggga tcctcagcgg cgacctctat tgcttttact acagaataaa
      721 gtggtatgct gccggacata tctatatcac ctgtaatcgg gtttatcgca aacttatcac
      781 ccttctgctc ggttacggga gctttgaagt ccaacaacaa gatactctct ttagcaataa
      841 tcggttcttc ggcagaaccc agcgatgcaa gaccgacaag catcccgata cctatgaaac
      901 cgaatattaa aaaagcaaga atagtgccta caaatgaggc taaaagcact tttaagaaat
      961 ctttcatatt tgaaaccttt gatttaatac ttaaaatata ttttgcaaag atacatatat
     1021 tttttacttt tgtcgcggtg aaaaaactgg caaaatatct atttagttca cttttaacga
     1081 tcctctatat tacggggttc gtaggttttg gagtgcataa atgccatact gaagggacgg
     1141 ctgatttgtt ccttatgatg ggcgatatct cctgcgagtc gattcaccat catattcatg
     1201 atcataacga ctgttgtgat gctcagtgtg actcccatcc attccattat acagactgta
     1261 gcagcgaatg ttgcagcacg gatgtttttg cagtcacttc cgaacaggat cgtttacagg
     1321 atgaagatat gggattatct gcacaaacaa ttgcagtttc ccttctctgt gatgatttga
     1381 cagctctctc ttacagcaca atcgtttccc cttccgggga tttgaatccg cttaaggtcg
     1441 gacttccact ctctgccctt tctatttgga ggttgtgatt tgcagctctc cggtgttgtc
     1501 gtgcggtcgc ttttgtgatc ccacgccata ccgtgtatcc atgccattaa atccgacaac
     1561 catttccatt tcaaaacaga ataaaaaaga gacataatga gaaaagcaat attttcagca
     1621 cttttcatcc ttttctgcac aaccattctt gcgcaggatt tcaagggttc aatattttac
     1681 aggagaagtg acggtaagac tgagccgctt ccctttgcac aggtctatct catagagaag
     1741 gctagactta tagagaccga tgagaacggt caatttacca tcaacctcga ggtgaggact
     1801 acaatggtgg ctacctacgt gggttacacc aaagatactt tgattgtgga tccgggcatt
     1861 ccttcgggag aattttacct gatgggagaa aacgaggtgg acgaagctgt agtaacctca
     1921 aggcaactca caaacaccct ctcacgcatc aaaccggtaa gaactgaagt aataacagca
     1981 gcaggtctct gtaaaatggc ctgttgcaac cttgcagaga gctttgaaaa ctccgccagc
     2041 gtcacggtag gttactcgga tgccattacc ggagcacgac agataaaact tctcggcttg
     2101 gcgggtacct atacccaact gctggatgag aacagaccgg tgatgagagg actcgcatct
     2161 ccttttggtc ttacctatgt ccccggccag tggctggaga gcatccagat tgccaaaggc
     2221 ccctcttcgg ttatcaacgg acttgaggcc atcacaggac agatcaatat ggaacatcgc
     2281 aaacctacag atgaagttcc cttctttttg cagctcttcg gtagcagcca tgccatggct
     2341 gagggcaata tagtctcatc cctccaatta aacgagaagt ggagtacagt gatcctcgct
     2401 cacgcctcat cgctgcttgt gggaagtgac cacaatgatg acggctttag ggatgaacct
     2461 caggctaccc aatttaatct ctccaataga tggctctact acgatccgag c
//
LOCUS       DAOSIE010000223         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_36097_length_2505_cov_3.848571, whole genome shotgun sequence.
ACCESSION   DAOSIE010000223 DAOSIE010000000
VERSION     DAOSIE010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36097_length_2505_cov_3.848571"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLI69_06235"
     CDS             complement(<1..563)
                     /locus_tag="PLI69_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOG42118.1"
                     /translation="MEQDTICALATPSGSGAIAVIRVSGNKSLEITDKIFKSSREGSL
                     REAKGYTVRYGTIFNEKEPVDKVLVTLFLSPASYTGEDSVEISCHNSPFIIKEILSLL
                     VKNGARMALAGEFTQRAFLNGKMDLAQAEAVADLIASETASAHRVALSQMRGGFSSQL
                     SSMRARLLEIVALMELELDFSEEEVEFA"
     gene            679..1518
                     /locus_tag="PLI69_06240"
     CDS             679..1518
                     /locus_tag="PLI69_06240"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOG42119.1"
                     /translation="MKRIEADIFQAVVINYDILHPSLNHSVLMRGLYLVLCHSGTISA
                     NANYESISCKAGDFFVIIPNTIFALKSVSKDYMAKAIYIPPKHITDITVALQDINLEQ
                     VLKFNKLTLDSQLNNDLESLHTIMMHQLEKPMTLYRRKLVLGLINSYIFIILGNINER
                     TGTADPNPSNKQMLARNFFITLLNGYREHKDVDYYAATLYVSSKYLSKVVKEITTFSP
                     QEWINKTLFMEAQRLLANTNLDITQIAEQLGYSSTSTFSRFFKQNTGLTPMEFRKQHT
                     TQE"
     gene            complement(1663..>2505)
                     /locus_tag="PLI69_06245"
     CDS             complement(1663..>2505)
                     /locus_tag="PLI69_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42120.1"
                     /translation="GMTAFYREMVKKGSSRYLSLNEAIIDIEKAAYKDFSSMDKAAVY
                     KGRGSINYNSSDTLFIHYQGGIMSSLYVDLVKRPFAGVYYDSIDYYYNFKMGQTTVID
                     GKLFYVVEFDQKTDDPDASILYRGRMFIESESLAIGRIEFAMNVEGRKEAASIFVPKK
                     PANLRVDVEQATYIVNFKEYDGLWYYDYARLEVKFSTRRKHAIFRNYYSVLSEMAVTD
                     HKRGDFKIDPAKRVRYKDMLSEKVKDFQDDDFWENYNIIEPDASIDAIIRRIIRQLSR
                     RENT"
BASE COUNT          727 a          561 c          465 g          752 t
ORIGIN      
        1 gcaaattcca cctcttcttc actaaaatcc agctccaact ccatcagcgc cactatttcc
       61 agcagacgag cccgcattga agagagttgc gaggagaatc cgcccctcat ctgagagagc
      121 gcaacccgat gtgcactcgc agtctccgat gcaatcaggt ctgcaacagc ttcggcctgc
      181 gcaaggtcca tcttgccgtt gaggaaggct ctttgggtaa attcccccgc taatgccatc
      241 ctggcaccat tttttaccaa cagagagagg atctctttga tgatgaaagg ggagttgtgg
      301 caggagattt ccacggagtc ttcacctgta tatgaggcag gggagaggaa tagtgtgacc
      361 aataccttat caaccggctc tttttcatta aatatggtac cataccggac ggtataacct
      421 tttgcctccc ttaatgagcc ttctctgctg cttttgaaga ttttatccgt tatctcaagc
      481 gacttattgc cactaactct tataactgct attgcaccgc ttcctgaggg ggttgccaga
      541 gcgcatatcg tatcttgttc catctgttcc attgttttta caaaaatagg caaaatgcat
      601 gcaattttag tggatttagg gatctttgtt tgaagtttta agataatttt cctatttttg
      661 gataaagaaa atgaagatat gaaaagaatc gaagctgata tctttcaggc ggtagtaatc
      721 aattatgata ttctccaccc gagtctgaac cactctgttc ttatgcgtgg tctttatctg
      781 gtattatgtc actcgggtac tatatctgcc aatgcaaact atgagtccat ttcttgcaag
      841 gcaggggact tttttgtaat aataccgaat actattttcg ctcttaagtc tgtcagcaag
      901 gattatatgg ctaaagccat ctatattccg ccaaagcaca taactgacat tactgttgca
      961 ctgcaagata tcaatctgga gcaagttctt aaattcaata aacttacgct tgattcccag
     1021 ctgaataacg acctggagtc tctgcatacc atcatgatgc accagctgga aaaacctatg
     1081 accctctata ggcgtaaatt agtgctggga ttgataaatt catacatttt tataattctg
     1141 ggaaacatca acgaacgtac aggaacagct gatccaaacc cctctaataa gcaaatgcta
     1201 gcacgaaact tcttcataac gcttttgaat gggtacaggg agcataaaga tgtcgattac
     1261 tatgcagcaa cgctctatgt ctccagtaaa tatctctcca aggtggtcaa ggagattacc
     1321 acattctcgc cccaggagtg gatcaacaaa actcttttta tggaagcaca aaggttgctt
     1381 gcaaatacta atcttgatat aacgcaaata gctgagcaac tcggatattc gtcgacatct
     1441 acattttcac gcttcttcaa acaaaatacg ggacttactc ccatggagtt cagaaagcag
     1501 cacacaactc aagaataacc cgcaatcgta ggatgctcga agagtaggaa agtgctcaat
     1561 cttaatctca atcttattct cttatataat taaataggat tctgccccct ttttatgcta
     1621 gcaaaatcct atttaacata atataacttg tgttaaaaag ggttatgtat tttcccttcg
     1681 actcaactgt cttataatcc tgcggataat ggcatctatt gaggcatcag gctcaataat
     1741 attgtaattc tcccagaaat cgtcatcctg aaaatctttt accttttcgg agagcatatc
     1801 tttatatcgt acgcgttttg ccggatcaat tttaaaatca cctctcttgt ggtcagtaac
     1861 ggccatttca gaaagcactg agtaataatt gcggaaaatc gcatgcttac gtctggttga
     1921 gaattttact tcaaggcgtg cataatcgta gtaccacaga ccgtcatatt ctttaaaatt
     1981 gacaatatat gtagcctgtt ctacgtcaac ccgcaaatta gccggctttt tgggaacaaa
     2041 gatacttgcg gcttccttgc ggccttctac attcattgca aactctatac gacctattgc
     2101 gagagattct gactctatga acatacgacc tctatataaa atagatgcat ccggatcatc
     2161 tgttttttga tcaaactcga ctacatagaa cagtttgcca tcaattaccg tggtctggcc
     2221 cattttaaaa ttgtaataat agtcaataga gtcataatag actcctgcga agggtctttt
     2281 cactaggtca acatagagtg acgacattat accaccctga taatgaatga ataaggtgtc
     2341 gctggagtta taattgatac taccgcgacc tttgtagacg gcagctttgt ccatagagga
     2401 aaaatcctta tacgcagcct tttctatatc tataattgcc tcattcagag acaaatatct
     2461 actactaccc ttttttacca tctctcggta aaatgccgtc attcc
//
LOCUS       DAOSIE010000224         2485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_36496_length_2485_cov_3.008230, whole genome shotgun sequence.
ACCESSION   DAOSIE010000224 DAOSIE010000000
VERSION     DAOSIE010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36496_length_2485_cov_3.008230"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PLI69_06250"
     CDS             <1..904
                     /locus_tag="PLI69_06250"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG42121.1"
                     /translation="GGHLAKWHKLFPEGESTEELLTSLTADMEGINCFTDTNIVSINK
                     LKNLYVAIFSNGISVQADAILFATGFSMFPAEKKEEYGYGIYNHVITNADLEHFFNTG
                     KDPRIKEPKVIGFVHCVGSRDEKAGNRQCSKVCCATALKQACEMKELYPEATVYCFYM
                     DLRMFGRKYEDLYLNAQRKYGILFVRGRVSEVSEDIDGRLVVKAEDTLVGKPIRITMD
                     LLVLMAGMSPCVENPKIASMTGVHIGEDGFFMPRDMLLRTTESHSKGIFFAGAATGPK
                     TIPETLNEARAAAMAIDNYFKQKA"
     gene            904..1230
                     /locus_tag="PLI69_06255"
     CDS             904..1230
                     /locus_tag="PLI69_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG42122.1"
                     /translation="MIDFGYSLTPSSTIEIDDADMSLFDELCAVEPDAIKCMSCGSCA
                     ASCTAGVFTDLNLRKVLLGLQRGCEEDVLPQLKSCMLCGKCHIVCPRGINTRHLILSI
                     CKIYNI"
     gene            1235..>2074
                     /locus_tag="PLI69_06260"
     CDS             1235..>2074
                     /locus_tag="PLI69_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG42123.1"
                     /translation="MMRERFISGYDHFVLPFMVGMIFVLLWCIIGVIKIMVQLERKDR
                     IKVYKSLVNPVIMGKNVRDWFGDCLFHVKLWKRNKVLGYMHSSIAFGWFMLIVIGHIE
                     TFLYIPERGGLFYYPIFFRYFMAVESITLKGSLLFFLMDFFLLVVLSGIILAIIKRFR
                     SKLMGMRRTTKLTLLDRVGLYSLWAIFPLRLIAEGFTAGVGGGSFLTVPVNWLFASIL
                     SKPENFTIAWWAYSIALGTFMMVLPYTRYMHIPAEFLLIPLKNAGIRVRRPRKGYALA
                     ELYS"
     assembly_gap    2075..2084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2085..>2485
                     /locus_tag="PLI69_06265"
     CDS             <2085..>2485
                     /locus_tag="PLI69_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG42124.1"
                     /translation="PSCGVCLDACPMSVDKAGLKNTTVYYIRQVKRRNQPRALAIADK
                     CLMCGKCSVLCPISISAIDLRLALRKQQPYNIEHDFSYLDKIPNGVLTSAISVGGEKV
                     LYYSGCMSSLTPMVSQSVLKSSYSRNSRALS"
BASE COUNT          654 a          570 c          571 g          680 t
ORIGIN      
        1 cggaggccat ctggcaaagt ggcacaaact ctttcccgaa ggtgagtcaa cggaggaact
       61 tctgacttcc ctgacagccg atatggaagg aatcaactgc tttaccgata ccaatatcgt
      121 ctctatcaac aaacttaaga atctctatgt ggctatcttt tccaatggca tttcagtaca
      181 ggcggatgcc attctttttg caacaggatt ttccatgttc cctgctgaaa aaaaggaaga
      241 atatggctac ggaatatata accatgttat tacaaatgcc gaccttgagc attttttcaa
      301 tacgggaaaa gacccaagga ttaaagaacc gaaggttata ggatttgtcc actgtgtggg
      361 ctcaagagat gaaaaagccg gcaaccgcca gtgctctaaa gtgtgctgcg ccacagctct
      421 caaacaggct tgcgagatga aggaactcta ccctgaggcc accgtttact gcttctatat
      481 ggacttgaga atgttcggtc gcaaatacga ggacctgtat ctgaacgccc agcgtaaata
      541 tgggatatta ttcgtccgtg gcagggtttc agaggtatca gaagatattg atggaagact
      601 tgtcgtaaag gcggaagata ctctggtagg caagcccata cgtattacta tggacctgct
      661 tgtactcatg gccggtatgt ctccatgcgt ggagaaccct aaaattgcct ctatgaccgg
      721 agtacatatc ggagaagatg gatttttcat gccacgcgat atgcttctac gcaccactga
      781 gagccacagt aaagggatat tttttgcagg tgccgccacc ggtcccaaga ctattcccga
      841 aactctcaac gaagccagag cggcagctat ggccattgat aactatttca aacaaaaggc
      901 ttaatgatag atttcggata tagtcttaca ccgagttcga caattgaaat agacgatgct
      961 gacatgagtc tatttgatga attatgcgca gtagaacctg atgccattaa gtgtatgtca
     1021 tgtggatctt gcgctgccag ttgtaccgcg ggtgttttta ctgatctcaa tctgagaaag
     1081 gtgctgctcg gccttcagcg cggatgcgaa gaagatgtgc tcccacaact caaatcctgc
     1141 atgctttgcg gtaagtgcca tatagtctgc cccagaggca tcaatacccg gcatctgatt
     1201 ctttcaattt gcaagattta caatatctga tactatgatg agggaaagat tcatatcggg
     1261 ttatgaccat tttgttctcc cattcatggt gggaatgata ttcgttctct tgtggtgcat
     1321 tatcggagtc atcaagatta tggtccaact cgagcgcaaa gatcgcatca aagtctataa
     1381 gtctttggtc aatcctgtga taatgggaaa aaatgttcgc gactggtttg gagattgtct
     1441 ttttcacgtt aaactctgga aacgcaacaa ggttctggga tatatgcact ccagtatcgc
     1501 tttcggctgg ttcatgctga ttgtcatcgg tcatatcgaa acattcctat atattcccga
     1561 aaggggtgga ttgttctatt atcccatatt tttccgatac tttatggcag tggagagcat
     1621 aaccctcaag ggctcgctac tcttcttcct catggatttt ttcctgcttg tggttctcag
     1681 cggtattatt ctggccatta tcaaacgttt ccgttccaag ttgatgggaa tgcgtagaac
     1741 tacaaagctc accctcctgg atcgcgtcgg actctattca ctctgggcaa tatttccatt
     1801 gaggctgata gcggaaggat ttaccgcagg tgtgggcgga ggcagtttcc tgaccgtacc
     1861 tgtcaattgg ctctttgcca gtatcctcag caaacctgag aatttcacga ttgcatggtg
     1921 ggcctactcg atagcgttgg gcacctttat gatggtgctg ccatatacca gatatatgca
     1981 tattccagcg gagttcctct tgattccact caaaaacgcg ggtatcagag tacgcaggcc
     2041 gcgcaaaggc tacgctttgg cagaactcta ctccnnnnnn nnnnccccag ctgcggagtt
     2101 tgcctggatg cctgccctat gagtgtcgat aaagcgggac taaagaatac caccgtctat
     2161 tatatacgtc aggtcaaaag aagaaatcag ccccgtgcac tggctatcgc cgataaatgt
     2221 ctgatgtgtg gcaagtgctc ggtcctttgc cctatcagta tatcagccat agacctgcgt
     2281 ttagcactcc gcaagcaaca gccctacaat atagaacacg acttcagcta ccttgataaa
     2341 atccctaacg gagtattaac atcagcaatc tcagtcggcg gagagaaagt gctctactat
     2401 tccggctgta tgagttcttt aacaccgatg gtctctcaat ccgtgttaaa gagctcatac
     2461 agccggaata gtagagcact ttctc
//
LOCUS       DAOSIE010000225         2483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_36525_length_2483_cov_4.247529, whole genome shotgun sequence.
ACCESSION   DAOSIE010000225 DAOSIE010000000
VERSION     DAOSIE010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36525_length_2483_cov_4.247529"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PLI69_06270"
     CDS             <1..713
                     /locus_tag="PLI69_06270"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOG42125.1"
                     /translation="EDLEFIMESVTLNKALVDEGLRNKYGLQVGRTLLAPINKVVFGD
                     TYLTYAMAYTAAASDARMAGCRLPAMSNSGSGNQGITATVPVIAIAEKLEASHEELAR
                     ALVISHLVAIHIKGHLGRLSALCGCVVASTGSACGIVYLRKGGYEQMCAAIKNMIGNI
                     TGMVCDGAKVGCALKVASGVASAVQSAVLALDGICISSNDGIIEEDIEKTIANLGRIG
                     SIGMQITDEMILEIMVCK"
     gene            complement(791..1450)
                     /locus_tag="PLI69_06275"
     CDS             complement(791..1450)
                     /locus_tag="PLI69_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG42126.1"
                     /translation="MMGRGEMKQRKYKHLIFDIDGTLLDTEKTGVLSLQKTVKELMGI
                     DMAYDELYPYFGIPTMVVAQKMGFKDVEAAAHSWEKNFQELMYLIVPFEGVSEMLPRL
                     REMGFKLGVVTSRSRFEFNYDPRLSQWKDLFETVITSDDSERHKPFPDPLLAYLSRSG
                     AASDECIYFGDTEYDFECAKGAGIDFALADWRNRGFQGIPAQYCFSSVEEIIKISETL
                     R"
     gene            complement(1447..>2483)
                     /locus_tag="PLI69_06280"
     CDS             complement(1447..>2483)
                     /locus_tag="PLI69_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOG42127.1"
                     /translation="EMYVTGSNTLSTQDDVAAALVKEGLNVFGWYNATAEEYEHHLCS
                     VLECGPNIIIDDGGDLTHLMHTRYTHLIDNVIGGCEETTTGINRLIRMAQTDTLKYPM
                     VMVNNANCKYLFDNRYGTGQSVWDGINRTTNLIVSGKNVVVAGYGWCGKGVAMRAKGL
                     GASVIVTEVEPVKALEAVMDGFRVMKMEEAAPIGDIFITVTGCKDVIEASSFEKMKDG
                     AICCNAGHFDCEVSVEQLSKMAIDSFETRTNVTGYKMRDGRCIYLLAEGKLVNLAAGD
                     GHPAEIMDMSFAIQALSARYLVKSRGKLPPSIVLVPEEIDNYVAQKKLKDLGVTIDEL
                     TKEQKAYLGL"
BASE COUNT          621 a          627 c          583 g          652 t
ORIGIN      
        1 ttgaagatct ggagtttata atggaatccg tgactctcaa caaagcgttg gtagatgagg
       61 gacttcgaaa taaatatggt ctccaggtgg gcagaacatt gcttgctccc atcaacaaag
      121 ttgtgtttgg agacacctat ctcacctatg ccatggctta cactgcggct gcttccgatg
      181 cgagaatggc tggctgtcgg cttcccgcta tgagcaactc gggcagcggc aatcagggta
      241 tcaccgctac ggttccggtt atcgctattg cagaaaaatt ggaggctagc cacgaggagc
      301 tggcaagggc attggtgatc agccacctgg tggctattca catcaagggg catctgggca
      361 gactctcggc tctctgcgga tgtgtggttg cttctaccgg ctcagcctgc ggcattgtct
      421 acctgcgcaa gggaggttat gagcagatgt gcgccgccat caaaaacatg ataggcaaca
      481 ttacaggcat ggtgtgtgac ggcgctaagg ttgggtgtgc tcttaaagtg gcttcgggag
      541 tggcaagcgc agtacaatcc gccgttcttg cgctcgatgg tatctgtata tcatccaacg
      601 acggcatcat agaggaggat atcgaaaaga ctattgccaa tctcggtaga atcggttcta
      661 taggtatgca gataacggac gagatgattc tggaaataat ggtatgtaaa taaagtgttg
      721 cggggtgcgg ggtggagttc tgagctccga gtatggagta ttgagccaag ggctaacgac
      781 cggcaactat ttagcgcagc gtttccgaga ttttaattat ttcttccaca cttgagaagc
      841 aatactgagc cgggattcct tgaaaacctc gattgcgcca gtcggcgagg gcgaaatcaa
      901 tccccgcccc tttggcacac tcaaagtcat attcggtatc tccgaaatag atacattcat
      961 cagatgctgc accggagcgg gacaaatagg ccaaaagcgg atcaggaaaa ggtttgtgac
     1021 gctccgaatc gtcactcgtg atgactgtct cgaaaagatc tttccattgt gacagacgcg
     1081 ggtcgtaatt gaattcaaaa cggctgcgtg aagtcaccac tcccaatttg aatcccattt
     1141 cgcgaaggcg gggaagcatt tcggagacac cctcaaacgg aacaatgaga tacatcagct
     1201 cctggaaatt cttctcccaa ctgtgtgctg ccgcctcaac atccttaaaa cccatttttt
     1261 gagccacgac catagttgga atcccgaaat aaggatagag ctcatcgtag gccatatcta
     1321 tgcccataag ctccttaaca gttttctgca aagagaggac accggtcttt tcggtgtcga
     1381 gcaatgtgcc gtctatgtca aaaatcagat gcttgtattt cctctgcttc atttctcccc
     1441 ttcccatcat aatcctaagt atgccttctg ctctttagtc agctcatcaa tagtcactcc
     1501 aagatcctta agcttctttt gggcgacata gttgtcgatc tcctccggaa ctaaaacaat
     1561 ggagggtggc aactttccgc gacttttaac caggtacctt gcgctgagtg cctgaatggc
     1621 aaaactcata tccataatct cggcagggtg tccatctccg gcagccagat ttaccaattt
     1681 cccctcagca aggagataga tgcatcgacc gtctctcatc ttatagccgg taacatttgt
     1741 acgagtctca aaactatcta tagccatttt tgagagttgt tccacggaga cttcacagtc
     1801 gaaatgacct gcattgcagc agatggcacc atctttcatc ttctcaaagc ttgaggcctc
     1861 gatcacatcc ttgcagccgg taactgtaat aaatatgtca ccaattggcg ctgcctcctc
     1921 catcttcatc acccggaagc catccatgac agcttccagg gcctttaccg gctccacctc
     1981 ggttacaatt accgaagctc ccagcccttt tgccctcatt gcaacgcctt ttccacacca
     2041 accataaccg gccactacta catttttacc tgaaactatg agattggtgg tgcgattgat
     2101 gccgtcccaa acggactgac ccgtaccata tctgttgtcg aataagtact tgcagttggc
     2161 attattgacc ataaccatag gatatttgag ggtatcggtc tgagccatcc ttattaagcg
     2221 gtttatgccg gtcgtggtct cctcgcagcc tccgataaca ttatcaataa gatgggtata
     2281 tcttgtatgc ataaggtggg tcaggtcgcc gccgtcatcg attattatat tggggccgca
     2341 ctccaacaca ctgcataggt gatgctcata ctcttctgcg gtggcattgt accatccaaa
     2401 gacattgaga ccctccttta caagtgctgc cgctacgtca tcctgtgtag atagcgtatt
     2461 actgccggta acatacattt cag
//
LOCUS       DAOSIE010000226         2482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_36550_length_2482_cov_3.340750, whole genome shotgun sequence.
ACCESSION   DAOSIE010000226 DAOSIE010000000
VERSION     DAOSIE010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36550_length_2482_cov_3.340750"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLI69_06285"
     CDS             complement(<1..372)
                     /locus_tag="PLI69_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOG42128.1"
                     /translation="MKLSELYVGQKESLSAQFSMEQVKTFAGISKDFNPLHLDPVYAA
                     QTIFKGNIVHGFLVGSLFSAILGTKMPGEGSIYLKQDMKFVKPVYINDTVSAVVTITD
                     INYEKQVVTLDTTCYIQVYVVV"
     gene            complement(433..1701)
                     /locus_tag="PLI69_06290"
     CDS             complement(433..1701)
                     /locus_tag="PLI69_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOG42129.1"
                     /translation="MKILHTSDWHLGQTFYDYDRTEEHKHFFEQLKKIVREEKPDLLL
                     VSGDLFHTSAPSIAAQELYNESLLGFRDESLAFGGMHIILIAGNHDSSSKLDVDGLLW
                     KYFNTDVVGTIPRDNDESLDNTSFYDSVFAKHIIPVRKRRESEILGYVVAVPHCYPQN
                     FPNINPEDGKQERMKRFIGVLLEKVDDINIEGLPVILTAHTLVTGSNIKGHDALIIGG
                     LDNSDIGTFGSGWDYLALGHIHFPQEIKGSNGKARYSGSPFAMNFEEDYDHFVNIVEI
                     EKRGDMPQVRQIPIKPLYEMVTLSAKELPEEKNSCMDLLRVLEEYPAHKKAYIRLEVL
                     ETGELPSDYIQRVKTALKGKKARYCLIKQELPDNPAEGEGLSLTAAQVGALSPREMAE
                     IIAPDLFGEAEEGYAKLFEQAVTNVSNQMR"
     gene            1889..>2482
                     /locus_tag="PLI69_06295"
     CDS             1889..>2482
                     /locus_tag="PLI69_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG42130.1"
                     /translation="MQPLAERMRPSKIEDFVGQEHLVAPGAVLRNMLKSKQLSSFILW
                     GPPGVGKTTLARIIAGSLDREFYTLSAVSSGVKDVREVIQHAESGRMFAKGAPILFID
                     EIHRFNKAQQDALLGAVESGTVVLIGATTENPSFEVISPLLSRCQVYVLKSLDVEHLK
                     VLLKRALKEDKILKQRKIKVKESEALFRISGGDARKLL"
BASE COUNT          670 a          583 c          509 g          720 t
ORIGIN      
        1 aacaactacg tagacttgaa tataacacgt agtatcgagc gtcaccactt gtttttcata
       61 attaatgtcc gtaatggtaa cgacagccga taccgtatca ttgatataaa caggttttac
      121 aaatttcata tcctgtttta aataaattga tccctcaccg ggcatcttcg taccgagaat
      181 ggcactaaaa agggagccta ctaaaaaacc atgtacaata ttgcctttaa atatcgtctg
      241 tgcggcatat acgggatcaa ggtgtagggg attaaaatct tttgaaatgc cggcaaaagt
      301 ctttacctgt tccatggaaa actgagcaga gaggctttct ttctgcccaa cgtacaattc
      361 acttaatttc ataaccattt aatttttata ataaatttca tttatctttc acttagcttg
      421 catagcctat ttttatctca tctgattact cacatttgtc acagcttgct caaaaagttt
      481 agcgtaaccc tcttcagcct ccccgaagag gtcaggcgca atgatttctg ccatttcacg
      541 cggagaaagc gcaccgactt gagccgctgt cagggagagg ccttcccctt cggcagggtt
      601 atcgggcagt tcttgcttta ttagacaata tcgtgccttt ttgcccttaa gagccgtttt
      661 aacgcgctga atgtagtccg aaggtagttc acctgtctca agcacttcta gccttatgta
      721 ggccttttta tgggcgggat actcttccag cacgcggaga agatccatac aactgttttt
      781 ttcctcaggg agctctttcg cagagagcgt caccatctcg tatagaggtt tgatgggtat
      841 ttgcctcacc tgaggcatat ctcccctctt ttcaatctcc acaatattta caaagtgatc
      901 ataatcctcc tcaaagttca tcgcaaaggg agatccgctg tatctcgctt taccattgct
      961 cccctttatt tcctgcggga aatgaatatg tcccagcgcc agataatccc atccgctacc
     1021 gaaagtgccg atatcggaat tgtcgagtcc tccgattatc agtgcatcgt gaccctttat
     1081 attgctccct gtcaccagag tgtgtgcggt caatatcacc ggaagcccct caatgttaat
     1141 gtcatctacc ttttccagca atactccgat gaatcgcttc atcctctcct gttttccatc
     1201 ttcaggattg atattgggaa aattttgggg ataacaatga ggcaccgcta ccacatatcc
     1261 gaggatctcg ctttctctgc gtttccttac gggaatgata tgcttggcaa agactgaatc
     1321 atagaatgaa gtattgtcaa gcgactcatc attatccctg ggaattgtcc ccaccacatc
     1381 cgtgttgaaa tatttccata aaagcccatc tacatccagt ttggagctgc tgtcgtgatt
     1441 gccggcaatc agaatgatgt gcatgcctcc aaatgcgagg gattcatcac ggaatccgag
     1501 caaagactca ttgtagagct cctgagcggc aatcgagggt gcagaggtat ggaagagatc
     1561 cccgctgaca agcagcagat ccggtttttc ttctcttact atttttttga gttgttcaaa
     1621 gaaatgttta tgctcctccg tcctgtcgta atcgtaaaaa gtctggccta aatgccaatc
     1681 gctcgtatgc agtatcttca tttcgatcta attttcggat gattcaaatt tgttaaagtt
     1741 agcaattatt cggaaatatc ataccgtata cccgccgaaa tcttttatta atatcatacc
     1801 ggacaattgt aaactatttc gttaatttct taccaagcaa ctacggaaat ctttccttat
     1861 atttgcagaa taaacaaata aaattatgat gcaacctctg gcagaacgga tgaggccttc
     1921 caagatcgaa gactttgtgg gacaggaaca ccttgtcgct cccggggcag tattgcgcaa
     1981 tatgctcaag agcaagcaat tgagttcatt catcctctgg gggcctcccg gagtgggtaa
     2041 aaccaccctt gcacgtataa tagccggcag tctcgataga gaattctaca ctctttcagc
     2101 tgtcagttcc ggagttaagg atgtacgtga ggtaatacag catgctgaaa gcggaagaat
     2161 gttcgccaaa ggtgctccaa tcctatttat tgatgagatc catcgcttca ataaagctca
     2221 gcaggatgca ttgctcggtg cggtcgagag cggcacagtt gtactgattg gcgctaccac
     2281 tgaaaatcct tctttcgagg taatcagccc actcctctcc cgttgccagg tgtatgtact
     2341 aaaatccctc gatgttgagc acctcaaggt actgctcaag agagctctta aagaagacaa
     2401 gattctaaaa cagcgcaaga taaaggttaa agagagcgaa gcacttttca gaatttcagg
     2461 gggggatgcc cgtaaattac tg
//
LOCUS       DAOSIE010000227         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_36861_length_2466_cov_2.249274, whole genome shotgun sequence.
ACCESSION   DAOSIE010000227 DAOSIE010000000
VERSION     DAOSIE010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36861_length_2466_cov_2.249274"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLI69_06300"
     CDS             complement(<1..380)
                     /locus_tag="PLI69_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOG42131.1"
                     /translation="MKPYALIFAAGLGTRLKPLTDTMPKALVPVDGVPLLERVITRLK
                     GAGYSNIVINAHHFAEQIIDFIEQKESFGISVAISDERDLLRETGGGIRHAAPLLKSD
                     HFLVHNVDIISDLDLPLFEEKYLEA"
     gene            complement(382..1797)
                     /locus_tag="PLI69_06305"
     CDS             complement(382..1797)
                     /locus_tag="PLI69_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOG42132.1"
                     /translation="MEQLKILFEQFTGEQPLSIEELPSSGSNRRYFRIKGEKRSLIGA
                     KGTCVDENIAFIKIADHFRKQGLNVPQVYNRSEDNIYYLQEDLGDDTLYRRITQGRES
                     GKYSHQEKELLLKTIRKLPAIQFDGAKGLDFSVCYPQPEFDERMIFFDLNYFKYCFLK
                     ATGIQFSEIELQNDFDKVSQVLMHSMSDTFLYRDFQARNVMIVNDEPYFIDFQGGRKG
                     PIFYDVASFIWQAKACYSENLKNELIDAYLDALQQYIPVDKREFRSTLRHFVLFRTLQ
                     VLGAYGFRGYFEKKPHFLQSVPFAIDNLRKLLVEPFPEYPYLANILGELTRMREFTEM
                     GVDRRLEVKIYSFAYKKGIPNDVSGNGGGYVFDCRALENPGKYDHFKHFTGLDKEVAD
                     FMEAEGGVQKFLTKVYSLADAHVQRYIERKFTHLMFCFGCTGGQHRSVYCAQHLAQHL
                     ADKFNIKIRLYHREQGLEQEF"
     gene            complement(1807..2394)
                     /locus_tag="PLI69_06310"
     CDS             complement(1807..2394)
                     /locus_tag="PLI69_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOG42133.1"
                     /translation="MNRISKEMTDIALPAPRRTGGMPLMEALNLRQSSRDLSNTPLDM
                     QTLSDLLWAAWGYNREHKRTAPSSHNRQEIDLYLFLESGTYIYDAAGNRLLLHDDEDL
                     RSLTGTQEFVGSAAVEIAMVADTSKIKGKTPQGTIEAIYANTGFISQNIYLFCAQAGL
                     GGVTRAMVPKAELAQRLRLNDSQIITLVHTVGYKK"
BASE COUNT          634 a          564 c          560 g          708 t
ORIGIN      
        1 gcttccagat atttttcctc gaagagaggc aagtccaaat ctgagataat atcgacattg
       61 tgaaccagga aatgatcgga ctttagcagg ggggcagcat gcctaattcc acctcctgtt
      121 tcacgcagca gatccctttc atccgaaata gctaccgaaa tcccgaatga ttccttctgc
      181 tctataaaat ctatgatctg ttctgcaaaa tggtgggcat tgatcacaat attggagtat
      241 ccggctccct taagacgcgt aatcaccctc tccagcaaag ggacaccatc aaccggcacc
      301 agtgctttcg gcatcgtgtc agtcagtggc ttgagtcgtg tgcccagacc tgcggcgaat
      361 atgagagcgt aaggcttcat cttagaattc ctgctccaga ccttgttccc gatggtagag
      421 gcgaatcttg atattgaatt tgtcggccag atgttgtgcc aaatgctggg cgcaatagac
      481 tgagcggtgc tggccaccgg tgcagccgaa gcaaaacata agatgggtaa attttctctc
      541 tatatatctc tgcacatggg catcggcgag agagtagacc ttagtaagaa atttttgcac
      601 cccaccttcg gcctccataa aatcagccac ctccttatcc agtccggtaa aatgcttgaa
      661 atgatcatac ttcccgggat tctccagagc gcgacaatcg aatacatatc cacctccatt
      721 gccgctgaca tcattgggaa tgcctttctt gtaggcaaaa ctgtagattt taacctcgag
      781 acgtctgtca acacccattt cggtaaattc tctcatgcgg gtcagttctc ccagaatgtt
      841 tgcaagatag ggatactcag gaaatggttc gaccaaaagt tttcgcaggt tatctatagc
      901 aaagggtacg ctctgcagga aatgtggttt tttctcgaaa taacctctaa aaccgtaagc
      961 tcccagtacc tggagagttc tgaaaagcac gaaatggcgt agtgtggagc gaaattccct
     1021 tttatccaca gggatatatt gctgaagagc atccagatag gcatctatga gttcattttt
     1081 caggttttcc gaataacagg ccttggcttg ccatataaag gatgccacat cgtaaaagat
     1141 aggtcccttt ctacctcctt gaaaatctat aaagtaaggt tcatcgttca caatcatcac
     1201 attacgggct tgaaaatccc gataaagaaa agtgtcggac atactgtgca tcagcacctg
     1261 agacacttta tcaaaatcat tctgcaactc aatttcgctg aattgaatgc ctgttgcttt
     1321 gagaaaacaa tacttaaagt agttgaggtc aaagaagatc atcctttcgt caaactcagg
     1381 ctgagggtaa catactgaga aatcgagtcc tttagctcca tcaaactgga ttgcaggaag
     1441 tttgcgaatt gtctttaata gcagctcctt ctcttggtga gagtatttgc cactctcacg
     1501 tccctgagtt attcgcctgt aaagcgtgtc atctccgagg tcttcttgaa ggtaataaat
     1561 attatcttca ctccggttat agacttgagg tacgtttagt ccctgttttc taaagtggtc
     1621 ggcaattttg atgaaagcta tattttcatc tacacaggtg cccttggctc caatcagact
     1681 gcgtttttct ccctttatac ggaagtaacg ccggttgctg ccggatgagg gaagttcttc
     1741 aatagatagt ggctgttcgc cggtaaattg ttcgaaaagt atcttgagtt gttccatttc
     1801 ggggtctcat tttttgtatc ctacagtatg gacgagtgtt atgatttgac tatcgtttag
     1861 ccgcaacctt tgtgccagtt ctgctttggg aaccatggca cgagtaacgc caccaaggcc
     1921 tgcctgggca cagaatagat agatattttg ggagatgaag ccggtattgg cgtaaatggc
     1981 ctctatagta ccctgaggag ttttcccctt gatttttgat gtgtcggcaa ccattgcaat
     2041 ttctacagcg gcactcccca cgaactcctg ggttcctgtg aggctgcgca agtcctcgtc
     2101 gtcgtgcagt aataatctgt tccctgcagc atcataaatg tatgttccgc tttcgaggaa
     2161 gaggtaaagg tctatttcct gccggttatg agaagagggg gcggtacgct tgtgttcccg
     2221 gttgtagccc catgcagccc agagcaggtc cgaaagtgtc tgcatgtcaa ggggagtatt
     2281 gctcagatcc cgggatgact gcctaaggtt aagagcctcc atcaggggca ttcctcctgt
     2341 gcggcgcggc gccggaagag ctatgtctgt catttcttta gatattctat tcataatttc
     2401 ctttgtttat caatgaattg aaattttata aatattatct ccacgggaac ctatgacaat
     2461 agtgtt
//
LOCUS       DAOSIE010000228         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_37495_length_2433_cov_3.835576, whole genome shotgun sequence.
ACCESSION   DAOSIE010000228 DAOSIE010000000
VERSION     DAOSIE010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37495_length_2433_cov_3.835576"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..1064
                     /locus_tag="PLI69_06315"
     CDS             366..1064
                     /locus_tag="PLI69_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42134.1"
                     /translation="MKLKLLLFALLALIVANCTLNDPAVPMENEGKEVTVSVTLSPET
                     RVLYDDSQVGNTPGALSWQTDDELMLIGFNDSDVYQGHRTFTYSGSGNTFTYTGQMPA
                     NATKYKAFYPASAVQLDANGDPVFNSNGNPPLPGTLFWQAAQTQSGISSTAHLRDRII
                     LRDTIANPLTESFGLAAMSSIMKVSLSGIPNDLGTLDKIFWSVGTSDEVYTRQRTLLV
                     TDVASGTTSMTACF"
     gene            1075..1809
                     /locus_tag="PLI69_06320"
     CDS             1075..1809
                     /locus_tag="PLI69_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42135.1"
                     /translation="MGIPANGKFKITLIGSAKSYDFIVTSAGKVYEAGKRYTANIPGT
                     SGIAWAQAFSEFRFTIHLGNSSSYTIYQKAASTSPANLTIEWGDGTTTDFAKGDQLQQ
                     AIASHNTYTMGNTYTITIRSDQADPSQKQLPELRFKGGGNSGDKEVKAILDPFPNMEA
                     TDFNQTFKNATNLTSIPSGLFRYNPNATSFRECFHSCNALTTIPERLFFYNPNATDFY
                     QCFYSCKNLVLVTTMFPNPATEPNFL"
     gene            1821..1997
                     /locus_tag="PLI69_06325"
     CDS             1821..1997
                     /locus_tag="PLI69_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42136.1"
                     /translation="MTWDQSFWSVGSNLAQGTGTAPQLWLFSTGGAWTTADCFKNATK
                     LSNYTDIPKAWGGK"
     gene            complement(2055..>2433)
                     /locus_tag="PLI69_06330"
     CDS             complement(2055..>2433)
                     /locus_tag="PLI69_06330"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOG42137.1"
                     /translation="KSAAPLGTQGGIGVVETGFTVIKSIRVTNQKGNYMKLKLKEGCV
                     VRKVGDNYVIIPRSGDNLNMMRMLRLNSSATFLLEQMQKGEFDREELVQLLMEKYAID
                     KATAEKDTDAFITKLREESLLEF"
BASE COUNT          706 a          580 c          506 g          641 t
ORIGIN      
        1 ggggagtttt tctattaaaa gaggaaatac ctcaatcaaa aaagtgctga tagcagtttt
       61 taagaaaaat tagactaaat ttgtgtaaat tttggcaaaa tatatcagca tattaaaaaa
      121 cttaatatat ttgtgcgttg ataggtaatt tttattaatt atgaacaaaa aagaattcaa
      181 atctgcagaa cagtacatcg cacccgctat cgaggtgatc gaagtcgaat tgagtcaaaa
      241 catcctccag tgcagcgctg gtgccgaatt gcctcctatc gatgatggag gaagtgcatg
      301 gtagcattaa attaacaact aatcaaagct gaaagctgac cggctacagt ttaaacaaga
      361 aagccatgaa gttaaaatta cttctttttg cactgcttgc cctaattgtg gcaaactgca
      421 ctctgaatga tcctgccgta cccatggaga atgagggtaa ggaggtcaca gtaagtgtaa
      481 ctctctcacc ggagacacgt gtattgtacg atgacagcca ggtcggcaac actcccgggg
      541 ctctctcgtg gcagactgac gacgaactca tgctgatcgg atttaatgac agcgatgttt
      601 atcaaggcca tcgcacattt acctatagcg gaagcggtaa cacgtttacg tatacgggac
      661 aaatgccggc aaatgccacc aaatacaagg ccttttatcc ggcaagtgcc gtccaactgg
      721 atgcaaacgg tgacccggta ttcaattcca acggcaatcc gccacttccc ggcactctat
      781 tctggcaagc ggcacaaaca cagtccggga tcagctcaac agcacacctt cgtgatagga
      841 taatcttgag ggatactatc gcaaatcccc ttacagagtc ctttggattg gctgctatga
      901 gcagtatcat gaaagttagt ctaagtggca taccaaacga tctgggaacc ctcgataaaa
      961 tattttggag tgttggaact tcggatgagg tttataccag acaacgtact ctcttagtga
     1021 cagacgtagc ttccggcacg acaagcatga ccgcttgctt ttgaccccaa tacgatgggt
     1081 atcccagcca atggaaaatt taaaataaca ctcatcggta gtgccaaatc ttacgatttt
     1141 atcgttactt cagccggtaa ggtctacgaa gcaggaaagc gttataccgc caatataccc
     1201 gggactagcg gaattgcttg ggcacaggcc tttagtgagt tcagatttac aatacatctt
     1261 ggtaatagca gttcttacac aatctatcag aaggccgcat ctaccagccc ggccaatctg
     1321 actatcgaat ggggagatgg tactacgact gacttcgcca agggcgatca acttcaacaa
     1381 gcaattgcca gtcataatac ctacaccatg gggaacactt ataccattac catccgttcc
     1441 gaccaagcag acccttccca aaaacagtta cctgaactac gtttcaaagg aggtggtaat
     1501 agtggagaca aggaggtaaa agcaatactt gaccccttcc ccaatatgga ggcaactgat
     1561 tttaaccaga ccttcaaaaa tgctacaaat ttgacatcta ttccttccgg attgttccgt
     1621 tataatccaa atgcaacaag tttcagagaa tgtttccact cttgcaacgc tttaaccact
     1681 attccggaga gactcttctt ttacaatcct aatgctacag atttctatca atgtttctac
     1741 agttgcaaaa atttggtgct ggtaaccaca atgtttccaa atccggctac ggaacccaat
     1801 tttttgtagc cagaggagct atgacttggg atcaaagttt ctggagcgta ggttcgaatc
     1861 tcgcgcaggg tacaggcacc gcccctcaac tctggttatt cagcacaggt ggagcgtgga
     1921 ctactgctga ttgcttcaaa aatgccacca agctaagcaa ctataccgat attccgaagg
     1981 cttggggagg taagtaacat aacctcttaa atagaaaaaa tcctcttacc tagtcatacc
     2041 ccgcaccgcg ggattcagaa ctccagaagc gattcttcgc ggagtttggt tataaaggca
     2101 tcggtgtctt tttcggcagt agccttgtct attgcatact tttccataag caactgcacc
     2161 agttcctctc tgtcaaactc ccctttttgc atctgctcca gcaggaaggt agcggagctg
     2221 ttgagtcgca gcatcctcat catgttgaga ttatcgccgc tgcgtgggat tatcacgtaa
     2281 ttgtccccaa cttttcgtac cacgcaaccc tcttttagct tcagtttcat atagttccct
     2341 ttttgatttg tcactctaat tgattttata acggtgaaac ccgtctccac aacgccaata
     2401 ccaccctgcg tacccaatgg ggctgccgac tta
//
LOCUS       DAOSIE010000229         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_37556_length_2429_cov_4.507582, whole genome shotgun sequence.
ACCESSION   DAOSIE010000229 DAOSIE010000000
VERSION     DAOSIE010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37556_length_2429_cov_4.507582"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PLI69_06335"
     CDS             complement(<1..892)
                     /locus_tag="PLI69_06335"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOG42138.1"
                     /translation="MKFKNSTLLIAALFIFTGNLFAQELYDRMEEQYKESCTSIMVAS
                     GASFDGSVMVGHTCDANYRTWLTIEPRKSFTIGDMEPVYEGMMHNEEPWDMRKVVEKG
                     RIPVVGTETYRFLNVAYPCLNEKQLAIGETTTEGRPELLNRAGIFYIEELQRMALQYC
                     ATAREAISLMGRLAEEYGYADWGECLTVADKNEVWHFEIYGSGPGNPSAMWVARRIPD
                     DHVGISANIPRIGKVDFNDKENFMYSRDLKERAKKTGFWDGKEDFIFHKVVSGRKPFS
                     IREFHVFSTLAPSLGLTYDVD"
     gene            1005..2099
                     /gene="carA"
                     /locus_tag="PLI69_06340"
     CDS             1005..2099
                     /gene="carA"
                     /locus_tag="PLI69_06340"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOG42139.1"
                     /translation="MQLLNRSRSIVQLENSLEFQGYSFGYMAPADGEVVFSTTMTGYP
                     ESLTDPSYEGQILCATYPLIGNYGVPEAITAGGVQQHFESDRIHVRGLIISDYSLKYS
                     HWSAIKSLDQWLRENRIPAIYGVDTRELTKVIRDDGSMLGRIVPEDASSQWDVEDPNT
                     TSLVARVSCKEVITYPGAIRSKRVVLIDCGVRHSILRYLISKNIEVIRVPWNYDFNDG
                     SVGRYDGLLISSGPGNPEFCVSTIEHIRHTMRLGKPIFGICMGHQLLARAAGARTFKL
                     KYGHRSHNQPVRATDSNRCYITTQNHGYAVDTTTLSKDWEPIFTNMNDGSNEGIRHKS
                     NKFFSTQFFPDMTGNPKDTSYLFDIFLTAL"
     gene            2099..>2429
                     /locus_tag="PLI69_06345"
     CDS             2099..>2429
                     /locus_tag="PLI69_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOG42140.1"
                     /translation="MDTSIKKVLLLGSGALKIGEAGEFDYSGSQALKAMKEEGIFTIL
                     INPNIATVQTSEGVADKIYFLPVTPYFVEKVIEKERPDGILLAFGGQTALNCGVKLFQ
                     AGVFGKYN"
BASE COUNT          685 a          598 c          528 g          618 t
ORIGIN      
        1 cgtctacatc ataagtgagg ccgagcgaag gtgcaagagt tgaaaacaca tggaattccc
       61 gaatggagaa gggttttctt cccgatacca ctttatgaaa aataaaatcc tctttcccat
      121 cccaaaatcc cgttttcttt gcacgctcct tgaggtcacg actatacatg aaattctctt
      181 tatcattgaa atccacctta ccgatgcggg ggatattggc tgaaattccc acgtgatcat
      241 ccggaatcct ccgggctacc cacatcgcac ttggattgcc tgggcctgaa ccgtatattt
      301 caaaatgcca tacctcattt ttatcagcga ctgttaggca ctctccccaa tcggcataac
      361 catactcctc tgccagacga cccatcaacg agatggcttc acgcgcagta gcacaatact
      421 gaagcgccat cctttggagc tcttctatat aaaagatacc tgccctgttg agcagctcag
      481 ggcggccctc agtagtagtc tcacctatgg ccagctgctt ttcattcagg cagggatagg
      541 ccacattcaa aaatctatat gtctccgttc ctaccacggg gatacgacct ttttccacta
      601 ccttacgcat atcccagggc tcttcattat gcatcatacc ctcatagacc ggttccatgt
      661 caccgattgt aaaactctta cgcggctcta tcgtaagcca ggtacggtaa ttggcatcac
      721 aggtatgtcc taccattacc gagccatcaa atgaggcccc ggaagctacc ataatggaag
      781 tacaactctc cttgtactgc tcttccatac gatcataaag ttcctgcgca aagaggttgc
      841 cggtaaagat aaaaagtgct gcaatcagca aggtggaatt tttgaatttc atatttaagt
      901 atttttccgg tttttataaa ctattaagtt cgcaaaaata gtcaaaaaag atacaagtcg
      961 caatgaaata attatctttg cacaatctta aaccgcatca aacaatgcaa cttttaaata
     1021 gatcacgatc tatcgttcaa ttggaaaata gccttgaatt tcagggctat tcgtttggtt
     1081 atatggcccc tgccgatgga gaggtggtgt tttccactac tatgaccggt tatccggagt
     1141 cccttaccga cccctcttac gaaggtcaga tactctgcgc aacatatccc ctgataggaa
     1201 attacggagt ccctgaagcc attacggccg gaggagtaca gcaacatttc gaatccgaca
     1261 ggatccacgt ccggggactt atcatatccg actactccct taaatatagt cactggagtg
     1321 caatcaagag tctcgaccaa tggctccggg aaaacaggat accggccatt tacggagtgg
     1381 atacccggga actgaccaag gtgatccgtg atgacggttc catgctcgga agaatagttc
     1441 ctgaggatgc atcctcccaa tgggatgttg aagatccaaa caccacgagc cttgttgcga
     1501 gagtgagttg taaagaggtc atcacctatc cgggagcgat acgctccaaa agagtggtgc
     1561 ttatagattg cggtgtcaga catagcatat tgagatatct gatttcaaaa aacatcgagg
     1621 tgataagggt cccgtggaac tatgatttca acgatggctc agtcgggaga tatgatggtc
     1681 tgcttatctc cagcggcccg ggcaatcctg aattttgtgt ttctacaata gaacacatac
     1741 gtcatactat gagactcggg aaacccatat tcggtatctg tatgggacac cagttgctcg
     1801 caagggctgc cggtgccaga acctttaagc taaaatacgg ccacagaagc cataaccagc
     1861 ccgttcgggc caccgatagt aacagatgct acattacaac gcagaaccat ggttatgcag
     1921 tagatacaac taccctcagc aaggattggg aacccatttt taccaatatg aacgatggct
     1981 ccaacgaggg aatccggcac aaaagcaata aattcttctc gactcaattt tttccggata
     2041 tgaccggaaa tcccaaagac acaagttatc ttttcgatat ttttctgacg gcactatgat
     2101 agatacaagc atcaagaaag tcctcctgct gggctccggg gcacttaaga taggtgaagc
     2161 gggcgaattt gactattcgg gctcacaagc tctcaaggcg atgaaagagg agggcatatt
     2221 tactatcctg atcaatccca atatcgccac ggtacagaca tccgaaggtg ttgcagacaa
     2281 aatctatttt cttcccgtga ctccttactt cgtagagaaa gtgatagaaa aggagcgtcc
     2341 ggatggtatt ctactggcat tcggaggcca gaccgctctt aactgcggag tgaagctctt
     2401 ccaggcgggc gttttcggga aatataacc
//
LOCUS       DAOSIE010000230         2402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_38150_length_2402_cov_4.063911, whole genome shotgun sequence.
ACCESSION   DAOSIE010000230 DAOSIE010000000
VERSION     DAOSIE010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38150_length_2402_cov_4.063911"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1115)
                     /locus_tag="PLI69_06350"
     CDS             complement(234..1115)
                     /locus_tag="PLI69_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42141.1"
                     /translation="MRSVLTRYFTLIFSIAVIACTLSCEPEENKETQSGLDALVTGYE
                     EKWLDYPAWEMKKSGEKLTGEGCTFLYWWKHRIEAKSAIELYAMCEIDNHLLSAMSTQ
                     NLVRSCYIYPYNGNVMAYCSLSLSFLDGIHYVMNIFNGYRELHNRKDAPREMLRLYKE
                     VKYSDRGDTFVVVLESKEYRKQGSIVGGISSFSLVMASAVDNECFTSAQIVELSKSVL
                     DKIDEITFDQAGTCSFHTAFYPYLLGAVISFHYDSELSESQSSILKKFIHDCNRHPVE
                     YSTIDLIYKSVSRIANR"
     gene            complement(1311..1394)
                     /locus_tag="PLI69_06355"
     tRNA            complement(1311..1394)
                     /locus_tag="PLI69_06355"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1358..1360),aa:Leu,seq:cag)
     gene            1538..1611
                     /locus_tag="PLI69_06360"
     tRNA            1538..1611
                     /locus_tag="PLI69_06360"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1572..1574,aa:Asn,seq:gtt)
     gene            complement(1680..>2402)
                     /locus_tag="PLI69_06365"
     CDS             complement(1680..>2402)
                     /locus_tag="PLI69_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOG42142.1"
                     /translation="YPPLVTPFSQYVKNTALMNLMHTLKGEPRWKTIDKDTMNMILGK
                     TGTLPGVLAPEILEIVKEKGLEFYTGNPQDEFPDELPKFEKMMEEEGWDRGQDDEELF
                     QFAMHERQYRDYKSGIAKERFERDLEAARAKAGAPVIVTRPVVEMPKFDVEKILEKFP
                     TAQPVQSTANGQLLWQYDVTQASSNPNIGDKVEAGRPVCFVQTYYGLEEIKANFDGKI
                     VMVAANHGDKVVKGEILAFIAK"
BASE COUNT          677 a          540 c          486 g          699 t
ORIGIN      
        1 ttggaaaaag acatcagtct gtaaggagga ttaatcatag tcctgcaagc ggaaaaagtt
       61 gccggcatca attcggcaac aaaattacct actctgggca cacctaccct atcattctcg
      121 aggataagct ctttaattgt gcgtgaaaaa aatgatacgt ccatcgtgaa aaaaatgata
      181 tgttcataac atacaaagtt agtaacaatc agcaataaaa acaacgactt actttaccta
      241 tttgcaattc tgcttacact tttatagatc aagtcgatag ttgaatattc taccggatga
      301 cgattgcagt cgtgaataaa tttcttaagg attgaggact gactttcaga gagttcgcta
      361 tcataatgga atgaaatgac ggcacccagc agatatggat agaacgcagt atggaaactg
      421 catgtccctg cctggtcgaa agtaatttca tcgatcttat caaggaccga tttgctaagt
      481 tcaacgattt gcgcagaggt aaagcattcg ttgtcaacag ccgatgccat aacaagcgaa
      541 aaagaagaaa ttcctcctac gatacttccc tgttttctat actctttact ctccaataca
      601 acgacaaaag tgtcgccacg gtcactatat ttcacctctt tgtagagacg caacatttct
      661 cttggggcat ctttgcgatt atggagttca cgatatccgt tgaagatgtt cataacataa
      721 tgaattccat ccagaaaaga aagggataga ctgcaatatg ccattacatt tccgttatat
      781 gggtaaatat aacagcttct tacaaggttt tgggttgaca ttgcagagag caaatgatta
      841 tcgatctcgc acattgcgta taactcaatt gcactctttg cttcaatcct atgtttccac
      901 caatataaga atgtgcagcc ctctccggtc agcttctctc cgcttttttt catttcccat
      961 gccggataat ctagccattt ttcttcgtaa ccggtaacaa gcgcatcaag gccggattga
     1021 gtctctttgt tttcttcagg ctcgcagctt aaagtacagg caattactgc aatcgaaaag
     1081 attaaggtaa agtatctcgt taaaacactc ctcatgattg gcatgctata acttaattat
     1141 tacaaatata gagaatattt ttaaagttct gagttctaag tgtgtagtat gtagtgttgc
     1201 tagtgcgggt tgcacggtac ggggtgcggg tgcgaggtgc taaatatgga aattcggagc
     1261 gaagcgactg acacaaaaag agtggtgaat tttcttcacc actctttttg tgcccggaac
     1321 aggggtcgaa cctgcacagc cttgcagcca ctagtccctg aaactagcgc gtctaccaat
     1381 tccgccatcc gggcattttt tagtttcagg ctgcaaatat aatcaaatcc gacaaatccg
     1441 ttaccaatta ttgttttttt acaattatta caacatttcg aatgtaatta ttttggtgat
     1501 caaagaaaaa tgttctacat ttgcagtccc aaaaccttcc tcaatagctc agtcggttag
     1561 agcacctgac tgttaatcag ggggtcctag gttcaagtcc tagttgagga gcatgaaaaa
     1621 tggaggccgg cgacactggc ctccattcta tttattacat ctcctgcatg acggagtcac
     1681 tatttggcta taaatgcgag tatctcaccc tttacaacct tatcaccgtg gttggcagct
     1741 accatcacga tcttgccgtc aaagttagcc ttgatctcct ccagaccata gtaggtctga
     1801 acaaaacata caggcctgcc tgcctctact ttgtcaccta tattggggtt ggaagaggcc
     1861 tgggtcacat catactgcca aagaagctgt ccgttggccg tagactgtac cggctgggcg
     1921 gtagggaatt tctcaagaat cttctcaaca tcaaatttag gcatttcaac aacagggcgg
     1981 gtaacgataa ccggggcacc tgctttggcc cttgcagcct ccagatctct ctcgaaacgc
     2041 tctttggcaa taccgctctt atagtctcgg tactgtctct cgtgcattgc aaactggaag
     2101 agttcttcat catcctgacc acgatcccaa ccctcttctt ccatcatctt ttcaaacttc
     2161 ggcagttcgt ccgggaactc atcctgagga ttaccggtat agaattcgag acctttctct
     2221 ttaacaattt ccaggatttc aggggctagt actccgggaa gggtaccggt tttaccgaga
     2281 atcatgttca tggtatcctt atcaatggtt ttccatctag gctcaccctt aagagtatgc
     2341 atcaggttca taagcgcagt attttttaca tactggctga aaggagtaac caatggagga
     2401 ta
//
LOCUS       DAOSIE010000231         2379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_38566_length_2379_cov_3.495697, whole genome shotgun sequence.
ACCESSION   DAOSIE010000231 DAOSIE010000000
VERSION     DAOSIE010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38566_length_2379_cov_3.495697"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1155
                     /locus_tag="PLI69_06370"
     CDS             124..1155
                     /locus_tag="PLI69_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOG42143.1"
                     /translation="MGRELRFVVDSAIPYIDILRDYADCTFCKASDITLEAVRDADAL
                     FVRTRTLCNETLLKDSRVSLVASATIGTDHIDLEWCSKHGIHVVSAQGCNSSAVRQYL
                     FTALYALAARKGISLQKPTLGVIGVGNVGSKVADFAHRLGWKVLLNDPPREMSEPQNA
                     HLFTPLNLLLAHSDIVTIHIPLWKNNYGFADSLFFENMKEKAIFVNTSRGEIVCEDAL
                     LSNVQRFSGVVADVWRGEPEINGEMLEAVDIATPHIAGYSRQGKINGSMAVVRATGEH
                     FGIDALKAFVVRDEGAVVRFNPEGLSQKKVCDRLCAIYDIWEDDRMLRANPGEFEKIR
                     SHYNYRTEF"
     gene            1159..>2379
                     /locus_tag="PLI69_06375"
     CDS             1159..>2379
                     /locus_tag="PLI69_06375"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOG42144.1"
                     /translation="MFTSEDLKVFAERGITVEQATKQMSRYETGFEWLDVLSAATPAK
                     GIKVFSENDKKAAFNAFVSNSLKVTKFVPASGAASRMFKDLFNVLESGEMSEYGTNFT
                     SRIKDFAFYTPEIFNGSTPMEIIDKVLNDSGLGYGSKPKGQLLFHRYSDDVRTAFEEH
                     LVEGALYAKMKDGTISIVISVSPEHLEGFKELSDSVKEKYEKRYGVKYSITFTLQSPS
                     TDTIAATEENKPFRKEDGSLLFRPGGHGALIKNLNEIDSDIVVIKNIDNVVKEDFIEQ
                     TVMWKKLLIGKLIILRERCFNYIERLDNALKVGCKDDIVPLCNEVAAFLEDQFCITLP
                     KTTPYELLPLLLRSKLDRPIRVCGMVRNLGEPGGGPFIVRDADGCTSLQILESVELNP
                     SDPRTAGLFATGTHF"
BASE COUNT          634 a          504 c          579 g          662 t
ORIGIN      
        1 tctcatgtaa gattgcttct tccgtacttc gcagtcagct tttttttcaa ctggaaagta
       61 taaagaagag gataaatacg atgcttcagg aagaacgtgt actcaagatt acgatttact
      121 gatatgggga gagaattgag atttgtggta gatagtgcta ttccctatat agacatttta
      181 agggattatg ccgactgtac cttctgtaag gcttcggata tcacactgga ggcggtgcgt
      241 gatgcagatg ccttatttgt acgtacccgc actctctgca acgaaactct gttgaaggac
      301 tcacgtgtct ccctggttgc cagcgccact ataggtaccg atcatattga tcttgaatgg
      361 tgctctaagc atggtataca tgtagtgagt gcccagggat gcaactcctc cgccgtcagg
      421 caatacctct ttaccgccct ctatgctctg gctgcccgca agggcatctc tttgcagaag
      481 cctacccttg gtgtcattgg agttgggaat gtcggctcaa aagtggccga ctttgcacat
      541 aggttaggat ggaaggtgtt gctcaatgat ccaccgaggg agatgtcaga gcctcaaaac
      601 gcccatctat ttaccccttt gaacctactg cttgcgcact ccgatattgt gactatccat
      661 atccctcttt ggaaaaataa ttatgggttt gccgactctc ttttctttga gaatatgaaa
      721 gaaaaggcta tttttgttaa cacttcccgt ggagaaattg tctgcgaaga tgcccttctg
      781 agcaatgtgc aaaggttttc gggtgttgtc gctgatgtgt ggagaggaga gccggagatt
      841 aatggggaaa tgcttgaggc ggttgatata gctacaccac atattgcagg ctattccaga
      901 caaggaaaga taaatggcag tatggctgtt gtgagagcta ccggagagca tttcggcata
      961 gatgccctga aagcatttgt cgtaagagat gaaggtgctg tagtcaggtt caatccggaa
     1021 gggttatcac agaagaaagt atgcgacaga ttgtgtgcca tttacgatat ttgggaggat
     1081 gacaggatgt tgcgcgccaa tccaggagaa ttcgaaaaga tacgttcaca ttataattac
     1141 agaacagaat tttgatttat gtttactagt gaagatttga aggtttttgc cgaaagaggc
     1201 attaccgttg aacaagccac caaacagatg tctcgttacg agaccggttt cgagtggctg
     1261 gatgtattga gtgccgctac acccgcaaag ggcattaagg tcttctccga aaatgacaag
     1321 aaagctgcat tcaatgcctt tgtttccaat tctctcaagg taaccaagtt cgtacccgct
     1381 tcaggggcgg cttccaggat gtttaaggat cttttcaatg tgttggaaag cggagaaatg
     1441 tccgagtatg gaaccaattt cacatcccgc atcaaggatt ttgcattcta cactccggag
     1501 atcttcaatg gatccactcc aatggagatc attgataagg tactcaatga cagcggcctt
     1561 ggatatggtt ccaaacccaa gggacagctc cttttccata ggtattccga tgatgtgcgt
     1621 acggctttcg aagaacatct ggtagaggga gccctttatg ccaaaatgaa ggatggtacc
     1681 atttccatcg tgatttcggt ttcaccggaa catctggaag gatttaagga gttgtcggat
     1741 tcggtcaagg aaaagtatga aaaacgttac ggagtaaaat attccatcac ttttacgctt
     1801 cagtcacctt ccactgacac tattgccgct acggaggaga acaaaccttt tcgaaaagaa
     1861 gatggttccc tgctcttccg tccgggaggt catggtgctc tgatcaaaaa tcttaatgag
     1921 attgactcgg acatcgttgt cattaagaac atagataatg tggttaagga ggattttata
     1981 gagcagactg taatgtggaa aaagctcctc attggaaaac tcatcatact ccgtgaacgc
     2041 tgttttaact atattgaacg cctcgacaat gcactcaaag ttggatgtaa ggatgatata
     2101 gtgccattgt gtaatgaagt cgcagctttt cttgaagacc aattttgcat tacattgccc
     2161 aaaacaactc cttatgagct gctccctttg ttgctccgct ccaagctcga ccgtccaatc
     2221 agggtttgcg ggatggtacg caatcttggt gaacccggtg gggggccttt catagttaga
     2281 gatgcagatg gctgtacttc gctccagata ctcgaaagcg tagagctcaa tccttcggat
     2341 ccccgtaccg ccggactgtt tgctaccggc acacacttc
//
LOCUS       DAOSIE010000232         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_38802_length_2370_cov_2.857883, whole genome shotgun sequence.
ACCESSION   DAOSIE010000232 DAOSIE010000000
VERSION     DAOSIE010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38802_length_2370_cov_2.857883"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2339)
                     /locus_tag="PLI69_06380"
     CDS             complement(<1..2339)
                     /locus_tag="PLI69_06380"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOG42145.1"
                     /translation="MKTLLTIIMAGALIFSPVTSSAQRNRQSNDSRIPVVKAEPKSDS
                     TKKAPQGPVSIEKFITKEASKMVGMTTAYKQDGKYYLTISDSLIGRDILMVSRVSQSA
                     ADIRGSFSGYAGDQIGSGLYRFEKGPDNRIFLRKVSVRERSQDSIMSMNILKSNLTPI
                     IGSFPIKAWNEDKSLSIIDVTDFFNADSEELYFSKRTKTSFKLGAQDKDKSYISSIRT
                     YPINTEVTVVKTFARTTGGNATYELNCSFVLLPEEPMTPRYYDERVGYFTTSYTDFDK
                     NPHGVEKVQLITRWRLEPRPEDLEKYKRGELVEPAKPIVYYIDPSTPAKWIPYLIQGV
                     NDWEPVFRNAGFKNAIRAEMAPTPEQDSTWSLEDARYSAIVYKPSDIANASGPHVNDP
                     RSGEIIESHINWYHNVMSLLHDWYFIQCSPVDEGARTMNFDDELMGQLVRFVSSHEVG
                     HTLGLRHNFVGSAIATTKQLRDPEYLKKYGHTTSIMDYSRFNFVVQPGDNIPRELLFP
                     RISTYDNWAIEWGYRRFYEYDDPKKELTMLNKWVIEKTFDPLYFFGTESSPNDPRYQS
                     EDLGNNQMETCELGIRNLKYVTDNLIDWTAEPNKDYEALRNMHNQVFSQYRRYIRHVA
                     KWVGGVYENPKRVEMEGDVYTPVEKTKQKEAMAFLNKQIFNPPAWLVPGDIINKVVTK
                     PEIIMDNAYKAVFGDLLSKRVMLNLYEAELQYGNKAYTMTDLFADLNKYMWDSPMPKE
                     ASAKAYKRMGQKVYVTLLCGLFTGETAIRATDTTKDHTD"
BASE COUNT          575 a          535 c          549 g          711 t
ORIGIN      
        1 atatccgtat gatcttttgt ggtatctgtt gcccttatcg cggtttctcc ggtaaagaga
       61 ccacaaagca gagtcacata gaccttctgt cccatgcgtt tatatgcttt tgcagaagcc
      121 tccttcggca tagggctatc ccacatgtat ttattgagat cggcaaacag atctgtcatg
      181 gtgtatgcct tatttccgta ctggagttcg gcttcgtaaa ggttgagcat tacccttttg
      241 ctgagcaggt ctccaaaaac tgctttgtag gcattatcca ttatgatttc aggctttgtt
      301 acaaccttat tgatgatatc gcccgggaca agccatgccg gtggattgaa tatttgcttg
      361 ttaaggaaag ccatagcctc tttttgcttg gttttctcca cgggagtata gacatcaccc
      421 tccatttcca ctcgtttggg attttcgtat actcccccaa cccatttggc aacatgcctg
      481 atatagcggc gatattggga aaacacctga ttgtgcatat tacggagtgc ttcatagtcc
      541 ttgtttggct cggctgtcca gtcaatgagg ttatctgtta catacttaag gttgcgtatg
      601 ccaagttcgc aagtctccat ctgattgttg cccaaatcct cgctctgata acgagggtca
      661 ttgggactcg attcggtacc aaagaaatag aggggatcga atgttttttc gattacccat
      721 ttgttgagca tggtaagctc ctttttagga tcgtcatatt cgtaaaatct cctgtaaccc
      781 cactctatag cccaattatc ataggtactt atgcgtggga acagcaattc acgaggaata
      841 ttatcacccg gctgaaccac gaagttgaag cgtgagtaat ccattatgga ggtggtatgg
      901 ccatattttt tcaggtactc ggggtcacgc aactgtttgg tggtggctat agccgaaccg
      961 acaaaattgt ggcgtagtcc aagagtgtgg ccaacctcgt gtgacgagac aaatctgacg
     1021 agttgtccca taagttcgtc atcgaaattc atcgtacgcg ctccctcatc taccggtgag
     1081 cactgtatga agtaccaatc atgcaggagc gacatcacat tgtgatacca gttgatgtga
     1141 ctctcgatta tttcacctga gcgggggtcg ttgacatggg gaccgctcgc atttgctatg
     1201 tcggagggtt tgtagactat ggcggaatat cttgcatcct ctaaagacca tgtagagtcc
     1261 tgttcgggag tcggagccat ctccgcacga attgcattct tgaaccctgc atttctgaaa
     1321 acaggctccc agtcatttac tccctgaata agatagggaa tccacttggc aggagtcgaa
     1381 gggtctatat agtagacaat aggcttagcg ggctccacaa gttcacctct cttatacttc
     1441 tcaagatctt caggtctggg ttccagtctc cagcgggtaa tcagctgtac tttctccaca
     1501 ccgtgaggat tcttgtcaaa gtcggtatag ctcgtggtaa aatagcccac tctttcgtca
     1561 tagtagcggg gagtcatcgg ctcttccggt agcagcacga aagagcagtt taattcgtat
     1621 gtagcattgc cgcctgtagt ccttgcaaag gtcttcacga ccgtcacctc cgtattgatg
     1681 ggataggtcc tgatgctgct tatgtaggat ttatctttgt cctgagctcc cagtttgaaa
     1741 gaggttttgg tcctcttcga gaaatagagc tcttccgagt ccgcattgaa gaaatcggta
     1801 acatctatga tagagagact cttatcctca ttccaggctt ttattgggaa tgagcctatg
     1861 atgggagtga gattggactt tagaatattc atgctcataa tggagtcttg tgagcgctcc
     1921 ctaaccgata cttttctgag aaagatccta ttgtcggggc ccttttcaaa tctgtagagg
     1981 ccactgccta tttggtcgcc ggcatacccg ctgaaactac ccctaatgtc cgctgcagat
     2041 tgagatactc tggaaaccat caggatatcc ctgccaatta gagagtccga aattgtgaga
     2101 taatacttac catcctgctt ataagcggtg gtcataccta ccattttcga agcctctttt
     2161 gtgatgaatt tttcaattga aacaggacct tgaggagcct tcttggttga atcgctcttt
     2221 ggttcagcct ttaccacagg tattctggaa tcattagatt gcctgttgcg ctgggcggag
     2281 gaggtaacgg ggctgaaaat caaagctccc gccataatga ttgtcaatag tgttttcatt
     2341 gtatctatgt ttgataaaaa tcaaaaaaat
//
LOCUS       DAOSIE010000233         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_38873_length_2367_cov_2.610727, whole genome shotgun sequence.
ACCESSION   DAOSIE010000233 DAOSIE010000000
VERSION     DAOSIE010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38873_length_2367_cov_2.610727"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="PLI69_06385"
     CDS             complement(<1..1390)
                     /locus_tag="PLI69_06385"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOG42146.1"
                     /translation="MASLKESIEQMFSRELSLHGRAFVNHQALSGMEVKEFDIDGYPA
                     RLLFNPAREASVMADISEETIRNRQCFLCEEGLSPEQLGTAWRSPATQEEYIFRVNPF
                     PIFDLHFTISLSYHKRQQIEGHFADMAAIARELPDYTIFYNGPMCGASAPDHLHFQAV
                     PAGNMPSEVVARKGEHLEPVYSCISGSISRFSIWSGGTYLLQSKSRSGIDTLFSRLMS
                     SAQIVDSTEWEPRVNILSWWEAGTYSTLVFFRRESRPACFSAEDPKERILISPASVEM
                     GGVAIVSSRESFNLLTADKLTSIIEEVSLDKISSQIMEQRLKRAQEELAVGIFSEEEI
                     EFSFITPYNAGGKIYKGDYRASVKNGKVLFDGELHDRIIFSSSEDNAAFKLKEVTIGV
                     NFHWERKEDQVFAGNLKLIVEKGKVTAINLIGIEDYLISVISSEMSATSSKQLLKAHA
                     VISRSWTLAQIVK"
     gene            complement(1395..>1663)
                     /locus_tag="PLI69_06390"
     CDS             complement(1395..>1663)
                     /locus_tag="PLI69_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG42147.1"
                     /translation="LGAPRAFYTPMLRTINFPTTKYGEDYAVGLRVSREYQIGRIYDV
                     LYVCRRWDDNSDASLDIEKVNANNLYKDRLRTWELKARVAMNRK"
     assembly_gap    1664..1673
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1674..>2367)
                     /locus_tag="PLI69_06395"
     CDS             complement(<1674..>2367)
                     /locus_tag="PLI69_06395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG42148.1"
                     /translation="MYDLRLKVSQKGDLEHINEYLYYDVETDTRKSGEKLFDYVDPKN
                     RAVQIEMEQACTEHLKVIGGYLKPKFRDVEFAEGKFEYEASVVIPCKNRVRTIKDAIT
                     SALSQKTNFKYNVIVVDDNSDDGTVEIIKSFKDNEKLIYIAQDKSYHAIGGNWNVAIH
                     HPKCGKFAIQLDSDDVYSDENSIQKIVDAFYEQNCAMVVGTYRMTNFHMETIPPGIID
                     HREWTPDNGRNNA"
BASE COUNT          551 a          550 c          586 g          670 t
ORIGIN      
        1 tcttgactat ctgtgcaagt gtccaggagc gggatattac tgcatgggcc ttaagcagct
       61 gttttgaaga ggtggcgctc atttcggagg agatcacgct gataaggtag tcttcgatgc
      121 cgatgagatt gatagcggtc accttgcctt tttccactat aagtttgagg ttgccggcga
      181 agacctgatc ttcttttctt tcccagtgga aattgacacc gattgtgacc tcctttagtt
      241 tgaaagcggc attgtcctca ctcgaggaga agattatccg gtcgtggagc tctccgtcga
      301 agagcacttt accattttta accgaagccc gatagtcgcc cttatagatc ttaccgccgg
      361 cattataggg cgtgatgaag ctgaattcta tttcttcttc agagaaaatg cctacagcaa
      421 gctcctcttg agcccttttg agtctctgtt ccataatttg tgatgatatt ttgtccaatg
      481 atacttcttc tatgatactt gttagtttgt cggcagtcag caaattaaag ctctctcgcg
      541 atgatactat tgctactcca cccatctcca ctgaagccgg actgattaga atacgctctt
      601 tagggtcttc cgctgaaaag caggcgggtc tggattccct gcggaaaaag accaaagtgg
      661 aataagtacc tgcctcccac caggagagaa tgtttacacg gggttcccac tctgtgctat
      721 cgacgatttg tgcgctgctc atcaggcgtg aaaaaagagt atcaataccg ctcctactct
      781 tactttggag cagataggta ccgcccgacc agatggaaaa acgacttatg ctgccggaga
      841 tgcagctgta aaccggttcc aggtgctctc ccttgcgtgc aacaacctcc gaaggcatat
      901 ttccggccgg aacggcctgg aagtggagat gatcgggcgc agaggcaccg cacatcggac
      961 cgttgtaaaa gatggtataa tccggcaatt cacgggcgat ggcagccata tcggcgaaat
     1021 gtccctctat ttgctgtctc ttgtggtatg aaagggaaat ggtgaaatga aggtcgaaga
     1081 ttgggaaggg atttaccctg aaaatgtatt cttcctgagt tgcagggctg cgccacgcag
     1141 tgccgagttg ctcaggagag agtccttctt cgcataggaa gcattgtcgg ttgcgaatgg
     1201 tctcctcgct tatatctgcc attaccgagg cttccctggc aggattgaag aggagtctgg
     1261 caggataacc gtcaatgtca aactctttga cctccatacc cgagagagcc tgatgattga
     1321 caaatgccct cccgtggagc gaaagttcac gggagaacat ctgttctata ctctctttta
     1381 gggaggccat atcgctactt gcggttcatt gcaacgcggg ctttaagttc ccaggtgcgg
     1441 agtcggtcct tatagaggtt attggcattt accttctcga tatcaaggga cgcgtccgaa
     1501 ttgtcatccc atctacggca tacatatagt acgtcataga tacggccgat ctgatattcg
     1561 cggcttacgc gcagacccac cgcatagtcc tcgccatatt tggtcgtagg aaaattgatg
     1621 gtgcgaagca tcggtgtgta gaaagctctc ggtgctccca ggcnnnnnnn nnngcattgt
     1681 tgcggccgtt atcgggggtc cactctctat ggtcgatgat tccgggagga atggtttcca
     1741 tatggaagtt ggtcatacga tatgtgccta caaccattgc acagttctgc tcgtaaaatg
     1801 cgtcaacaat cttctgaata ctgttttcat ccgaatagac atcgtcggag tcgagttgga
     1861 tggcaaattt tccgcatttg ggatggtgga ttgccacatt ccagttgccg cctatggcgt
     1921 gataagactt atcctgagca atgtagatga gtttttcatt gtctttgaat gacttgatga
     1981 tctctactgt accatcgtct gagttgtcat ccacaactat aacattgtat ttgaagttgg
     2041 ttttctggct gagagctgaa gtgatggcgt ccttgatggt gcgcaccctg tttttgcagg
     2101 gaataacaac ggaagcctca tattcgaatt tgccttcagc aaattccaca tccctgaatt
     2161 tgggcttgag gtatccgcct ataaccttca gatgttccgt acaggcctgt tccatctcaa
     2221 tttgtacagc cctgttcttg gggtcaacat agtcaaacaa tttctcgccc gatttgcggg
     2281 tgtctgtttc gacatcgtaa tagagatatt catttatatg ctcaaggtcg cctttctgcg
     2341 aaactttgag gcgcagatcg tacattg
//
LOCUS       DAOSIE010000234         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_38891_length_2366_cov_3.211597, whole genome shotgun sequence.
ACCESSION   DAOSIE010000234 DAOSIE010000000
VERSION     DAOSIE010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38891_length_2366_cov_3.211597"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /locus_tag="PLI69_06400"
     CDS             complement(<1..1685)
                     /locus_tag="PLI69_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOG42149.1"
                     /translation="MKHFRSALAALLILLTFAPSVGAQEKKSLTNAEILGNMPKGIVG
                     QPAIPMAFDDNRTLIYREGQEFFRYDLRKGTAEPFSYTPPRGTSRFEELVPGWKNPTF
                     SPDSTKIAYTLDNDLYSINVKSKKIIRHTFDGSELNLNGYASWVYYEEIFGRGSNYRA
                     FWWSPDSKVIAYYNFDNSQVPMFPIYNSQGQHGSLTQTRYPKAGDSNPKVRIGFVSAN
                     GGETVWSDFDPEPDQYFGIPFWSGDGKRFMVARMDRAQDNLELFSVDPILGNFVCVYA
                     EHQESWIDWMEEMLFTPEGIYIVRDFEGWEQIYFLSYDGNRFEKLTGGRNWGISLLKV
                     DSKYLFFTAKREATTRVDVYRVTLRSQKTERVSFDNYNFANVIISPDSRNVAAVVSNA
                     QTPYFLVNISLPTGNLNRKKVTVISDSKGPDFDKYDIALPEIITIKTRDGLDIPASVI
                     WPVNLDKSKKYPVKVNIYGGPNSPQVRDRWSGVSFSNQWWANNGVIQVTLDNRAGGHF
                     GKKGLEQVYRRLSIPELRDFIDGIKHFLAMPFVDADKIGIEGFSYGGMMTVLCV"
     gene            complement(1687..2364)
                     /locus_tag="PLI69_06405"
     CDS             complement(1687..2364)
                     /locus_tag="PLI69_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOG42150.1"
                     /translation="MNIMYWILFIAVAVVSFAVQYNLKRKFEKYSRIPLGSGMTGAEV
                     AERMLRQNGINDVKVTAVGGQLTDHFNPLTKTVNLSEGVYHSNSVAAAAVAAHECGHA
                     VQHANGYIPLKMRSALVPVVSFASKIMSWVLLLGMFLINVFPQVLMAGIALFALTTLF
                     SFITLPVEINASKRALVWLNTTGITDISNHEHAEKALRAAAYTYVVAALASLATLVYY
                     IMLANRR"
BASE COUNT          638 a          556 c          517 g          655 t
ORIGIN      
        1 acacagagaa ctgtcatcat gccaccatat gaaaagccct ctatacctat tttgtcagca
       61 tccacaaatg gcattgcgag aaaatgtttg ataccatcaa taaagtcccg gagttcggga
      121 atactgagtc gtctgtaaac ctgctcaagt cccttttttc cgaaatgacc acctgctcgg
      181 ttgtccaaag tcacctgtat cactccattg ttagcccacc attggttgga aaatgagact
      241 ccgctccatc tgtcacgcac ctgcggggag ttgggacctc cgtaaatatt aacctttacg
      301 ggatattttt tacttttatc caggttgaca ggccagatca ctgaggctgg aatatcaagc
      361 ccgtcacggg ttttaatggt gatgatctct ggcagagcta tgtcatattt gtcgaagtcg
      421 ggaccttttg agtctgaaat tacagttact ttcttcctat tgagatttcc tgtaggaagt
      481 gaaatattta ccagaaaata aggggtttga gcatttgaaa cgactgccgc cacattgcgg
      541 ctgtcgggag agatgatgac attggcaaaa ttatagttgt caaaagagac tctttcggtc
      601 ttttgactcc ttagggttac tctgtataca tccactctcg tggttgcctc tctcttggca
      661 gtaaagaata gatattttga atccaccttg agcagactta taccccaatt tcgacctccg
      721 gtaagttttt caaaacgatt accgtcataa gagaggaagt agatctgctc ccatccctcg
      781 aaatccctta caatatagat accttcagga gtaaaaagca tctcctccat ccaatcaatc
      841 caactctcct gatgttctgc atatacgcag acgaagtttc ctaatatggg gtcaactgaa
      901 aagagttcaa gattgtcttg tgcacgatcc attcttgcaa ccataaaacg cttgccatca
      961 ccgctccaga agggtatacc gaagtattgg tctggctccg ggtcgaagtc ggaccagaca
     1021 gtctctcccc cgtttgccga tacgaagcct atcctaacct tgggattgga atcaccggcc
     1081 ttggggtaac gggtttgagt aagtgaaccg tgttgacctt gtgaattata aatgggaaac
     1141 atgggcacct gagagttgtc gaagttgtaa taggcaatta ccttactatc gggagaccac
     1201 caaaatgccc tgtaattgga gcctctgcca aatatttcct cataatagac ccaggaggca
     1261 tacccattta gattgagttc cgacccatca aatgtatgcc ttatgatctt tttactcttc
     1321 acatttatac tatacagatc gttatctaga gtatatgcta ttttagtgga atcgggagag
     1381 aatgtgggat ttttccaacc cggaaccaat tcttcaaaac gtgaagttcc tctaggtgga
     1441 gtatatgaga aaggttcggc agtccccttt cggaggtcat atctgaaaaa ttcctgaccc
     1501 tccctataaa ttaatgtcct gttgtcatca aatgccatcg gtatggcagg ctgtcccact
     1561 ataccttttg gcatattgcc cagaatctcc gcatttgtga gactcttttt ttcctgcgcg
     1621 cctacagagg gagcaaaggt taaaagaata agcagcgccg ctaatgctga tctgaagtgt
     1681 ttcatattat cttctgtttg caagcattat gtaataaacc agggtagcca gtgaggccag
     1741 ggcggccact acataggtat aagcagcggc acggagggcc ttttcggcat gctcatggtt
     1801 agaaatatct gtgatacctg tggtattgag ccatacaagg gccctcttgc tggcgttgat
     1861 ttccaccgga agtgtaatga aactgaatag tgtcgtaagt gcaaacagcg ctatgccggc
     1921 catcagtacc tgtggaaaga cattgatgag aaacataccc aacaaaagta cccacgacat
     1981 tattttggat gcaaaactca ccaccggcac caatgccgag cgcattttga gcgggatgta
     2041 accatttgcg tgttgcacgg cgtgcccgca ctcgtgagcg gctacagctg cagcagccac
     2101 cgagttggaa tggtatactc cctcgctgag attgacagtc tttgtcagag ggttgaaatg
     2161 gtctgtaagt tgaccaccga cagctgtcac cttgacatca tttatgccat tttggcggag
     2221 catcctctcc gcaacctccg ctccggtcat acctgaaccg agaggtatcc gggagtactt
     2281 ctcaaatttt ctcttaaggt tatactgaac ggcaaaactg actaccgcga cagcaataaa
     2341 taaaatccaa tacataatat tcattc
//
LOCUS       DAOSIE010000235         2342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_39419_length_2342_cov_2.810669, whole genome shotgun sequence.
ACCESSION   DAOSIE010000235 DAOSIE010000000
VERSION     DAOSIE010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39419_length_2342_cov_2.810669"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLI69_06410"
     CDS             complement(<1..208)
                     /locus_tag="PLI69_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42151.1"
                     /translation="MNKKNIRVIILAMAIFAAFFSGKKQERPERDKVEEIIAKMSLRE
                     KVGQLFIIEYSDKYDLEDPYFDKLI"
     gene            complement(224..>2342)
                     /locus_tag="PLI69_06415"
     CDS             complement(224..>2342)
                     /locus_tag="PLI69_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOG42152.1"
                     /translation="QEGMFNIDKHTIIIPEREGSFSATYLQQHISPIFEYGIALGKAS
                     DNPKDNAIVLRKREGFKPESYSLTICNRGVRINASDDAGLFYGVQTLLQMFPQAVYSG
                     EKLRLREYSLQNVIIEDSPRFSHRGFMLDVSRTFFDKEYVKRYLDWMAIHKLNVFQWH
                     LTDDNGWRIEIKKYPQLTEKGAWRGRNEIIPPTYLSGNERYGGYYTQKEIKEVVAYAA
                     QRNITIIPTIDLPGHSLSSTTVLDGVTCGTTTDKASACGEFDNVWCVSKESNYKILDN
                     IMKEVASLFPGPYISIAADEVVWDYWAQCPGCQALMEKEVYKEVPELLGHFVRRMETI
                     INKHGKQLAGFDDIQDAGGVSNNALVIAWRGYKKAQESVSKGYPTVMQLGEYCYLDMQ
                     YSPAERGHNWAAVIPLDRIYNYDPTGGLGELTPEQEKLVLGPQGGLWTELMQYPPRFA
                     EYQVFPRLAALAEVGWSSKEKKDYEDFHRRLVSSHYGRLEKMGIAFRVEPPIVKYRDQ
                     QIEVILPYSSAVVRYTTDGTDPVNTSKVLSGTIITDSPEKFRFATFWGNNLQSISVAP
                     EGVVLNRWIQPAVRVETDIKMKSNTPLTNITGYNYKLIARSDKRLEAGQTLTYIFDEP
                     VECSKIGVPTGYAHIPFYGVSDGYVEYSYDGVNFIKGADFKRYYAEITGFEAPVKAVR
                     IVVTMPNDGSTCCFQNLRIVGSM"
BASE COUNT          574 a          592 c          498 g          678 t
ORIGIN      
        1 ttataagttt gtcgaaatat gggtcctcaa gatcatattt gtcgctgtat tctattatga
       61 aaagttgtcc taccttctct ctgagggaca ttttggcgat gatctcttcc accttgtccc
      121 gctccggacg ttcctgcttt ttccccgaaa aaaatgctgc aaaaatggcc attgccaata
      181 ttataactct tatatttttt ttattcatac ttcacactac ttactacata ctacccacta
      241 ttctcaggtt ttgaaaacag caggttgagc cgtcgttggg cattgtgaca acaatgcgta
      301 ctgcctttac cggtgcttca aagccggtga tttcggcata atatctcttg aagtcagctc
      361 ccttgatgaa atttaccccg tcataagaat actccacata gccgtcacta acgccataaa
      421 atggaatatg tgcataacct gtaggaactc caatcttact gcactcgacc ggctcgtcaa
      481 aaatgtaggt tagagtctga ccggcctcca aacgtttatc gctgcgggcg atcagtttat
      541 aattataacc ggtgatattg gtcagaggag tattggattt catcttgata tcggtctcta
      601 ccctgacagc aggttgtatc catcggttca gtacgacacc ctccggagca acggagatac
      661 tttgcaaatt attgccccag aaggtagcaa aacggaattt ctccggcgaa tcggtaatta
      721 tggtaccgct caatactttc gacgtgttta ctgggtcggt accgtcagta gtatagcgta
      781 caaccgctga ggaatagggg agaatcacct ctatctgctg gtccctatat tttacaatgg
      841 gtggttccac tcgaaatgca atacccattt tctccaggcg gccatagtga ctgctcacaa
      901 gtctgcggtg aaaatcctca tagtccttct tttccttgct gctccagcct acctctgcaa
      961 gcgctgccag acgcgggaag acctgatact ctgcaaaacg gggagggtac tgcatcaatt
     1021 ctgtccaaag acctccctga gggccgagta cgagcttctc ctgttccgga gtgagctcgc
     1081 ccagtccacc tgtaggatca taattgtata tgcggtccag cggaattact gctgcccagt
     1141 tgtgacctct ctccgccggt gaatattgca tatccagata gcagtactct ccaagctgca
     1201 ttactgtggg ataaccctta ctaaccgact cctgcgcctt cttatatccc ctccaggcta
     1261 ttacgagtgc attgttgctc actcctcctg catcctgaat gtcgtcaaat ccggcgagct
     1321 gctttccatg cttattgata attgtttcca ttctgcggac gaagtgacca agaagctcgg
     1381 gaacctcttt gtagacctct ttctccatta gtgcctggca accgggacat tgtgcccaat
     1441 aatcccatac aacttcatct gctgcaatac tgatgtaggg tcccgggaag agcgatgcta
     1501 cctccttcat gatattatcg aggattttat aattggactc cttgctaaca caccatacgt
     1561 tgtcgaactc tccgcaagca cttgccttgt cggtagtagt accgcaggtt acaccatcca
     1621 atactgttgt agaggagagg gagtgtcccg gaaggtcgat tgtcggtata atggtaatat
     1681 tgcgctgagc tgcataagca accacctctt taatctcctt ttgcgtataa taaccgccgt
     1741 agcgctcatt gcctgaaagg tatgtagggg gaataatttc gttgcgacct ctccaagctc
     1801 ctttttccgt cagctgcgga tatttcttta tctctattct ccaaccattg tcatccgtca
     1861 gatgccattg gaaaacattt agtttgtgaa tggccatcca atccaaatag cgcttgacat
     1921 actctttatc gaaaaatgta cgggaaacat ccagcataaa tcccctgtgg gagaatcgcg
     1981 gcgaatcctc aataatgaca ttttgtagcg aatattcacg caaacggagt ttttcgccac
     2041 tgtagaccgc ctgagggaac atctgtaaca gcgtttgaac tccgtaaaaa agccctgcgt
     2101 catcggaggc attgatcctc actccgcgat tacagatagt gagtgagtaa ctctccggct
     2161 tgaatccctc tctcttacgc agtacaatag cgttatcctt agggttgtca gaggccttgc
     2221 ctagtgcaat cccatactcg aaaataggac ttatgtgctg ttggaggtat gttgcgctga
     2281 aactaccttc tctttcgggg atgatgatag tgtgcttatc tatgttgaac ataccctctt
     2341 gc
//
LOCUS       DAOSIE010000236         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_39720_length_2328_cov_4.315002, whole genome shotgun sequence.
ACCESSION   DAOSIE010000236 DAOSIE010000000
VERSION     DAOSIE010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39720_length_2328_cov_4.315002"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLI69_06420"
     CDS             complement(<1..472)
                     /locus_tag="PLI69_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42153.1"
                     /translation="MIQKMTRYSIIAFQPELMDFLDQLQELGVMDITRSFKATDETSS
                     HLIEITTSEKKLIKRYKEFLKNLPEETPVKEIIKPEADTDIFERIDTLLDSSISLKQE
                     LTTAIRERNDALPWGDFDPADVDRLQKAGFTPHFHMIPDKKFNPDWEKEYPLQLL"
     gene            complement(469..1080)
                     /locus_tag="PLI69_06425"
     CDS             complement(469..1080)
                     /locus_tag="PLI69_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOG42154.1"
                     /translation="MAIKFQFNKTSLNEINKQLKIRLLALPTLKNKESALRLEVKKAK
                     KRSEELVEELERALQHYDYLAALWHEFEPGLIKVKDVDLATVKVAGVKTPELKSVSYD
                     VTEFNRYTSPLWFSEGVEILKKLAQLGIESEVYVEKSRILDFQRKKTTQKVNLYEKVQ
                     IPGYQEAILKIKRFIEDEENLSKAAQKIVKARHQAEEEEEEAS"
     gene            complement(1084..>2328)
                     /locus_tag="PLI69_06430"
     CDS             complement(1084..>2328)
                     /locus_tag="PLI69_06430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOG42155.1"
                     /translation="GVTNDELAIVAGRLAQVVKIKGNLVTLQIFEGTEGIPTNAEVLF
                     FGEAPALNVSDDLAGRFFNAYGEPIDDGPKVEGERRYIGGPSVNPFRRKQPSQLIPTG
                     IAGIDLNNTLVSGQKIPFFADPDQPYNQVMAQVALRADVDKIILGGMGLSNDDYLYFK
                     QMFEGAGALNKIISFVNTTEQPPVERLLIPDMALAAAEYFAFDKNEKVLVLLTDMTLY
                     ADALTIVSNRMDQIPSKDSMPGSLYSDLAKIYEKAVQLPDGGSITIIAVTTLNDGDIT
                     HAIPDNTGYITEGQLFLRMDTDTGKVIIDPFRSLSRLKQLVIGKQTREDHNQVMNTAV
                     RLYADAANAKTKLENGFDLSDYDLRCLDYAKEYSTRLLAIDVNISIDEMLDTAWELFG
                     KYFSKVETGIRESLVEKYWKEQ"
BASE COUNT          545 a          528 c          550 g          705 t
ORIGIN      
        1 tgagcaactg caaaggatac tccttctccc aatccggatt gaattttttg tccggtatca
       61 tatggaagtg aggtgtaaat ccggccttct gaagacggtc aacatccgca gggtcaaagt
      121 ctccccatgg gagcgcatca tttcgttcgc gtattgcggt agtgagctcc tgcttcaagg
      181 aaattgaaga gtcgagcaga gtgtcgattc tttcaaaaat gtctgtatct gcctccggct
      241 tgataatttc cttgacaggt gtctcttcgg ggaggttttt gagaaattcc ttgtagcgct
      301 ttatcagctt tttctctgac gtggtgattt caataagatg actggaagtc tcgtcagtag
      361 ccttgaaaga gcgggtgata tccattactc ccagctcctg gagctggtca aggaaatcca
      421 tcagttcggg ttgaaatgca atgatggagt atcgtgtcat tttctgtatc atgatgcttc
      481 ctcctcttct tcttcagctt ggtggcgtgc cttgactatc ttttgtgcag ctttggagag
      541 attctcctca tcctcaataa atcgttttat tttgagtatt gcctcctgat atcccggaat
      601 ctgtaccttc tcatataggt ttactttctg tgtggtcttt ttcctttgaa aatccagaat
      661 tctgctcttc tccacatata cctcagactc aataccgagt tgtgcgagct tcttaagaat
      721 ttctacacct tctgaaaacc acagaggact cgtataacgg ttaaattcag taacatcata
      781 acttacactc ttcaattcgg gagtcttgac tccggcaacc tttacagtcg ccagatcaac
      841 atccttaacc tttatcaagc cgggttcaaa ttcgtgccaa agtgcagcga gatagtcata
      901 gtgctgcagc gctctctcca actcctccac gagctcctct gaacgtttct tagccttctt
      961 tacctccagg cggagtgccg actccttgtt tttaagggtt ggtagggcca gtaaacggat
     1021 cttcaattgc ttgttgatct cgttcaggga tgttttgttg aattggaatt tgatagccat
     1081 aagttattgt tctttccagt atttttctac aagtgattcc ctgatgcctg tctccacttt
     1141 ggagaaatat ttaccgaaga gctcccaggc ggtatcgagc atctcgtcaa tggagatatt
     1201 gacgtcgata gcgagtaaac gggtagaata ttccttggcg taatcgaggc agcgcaggtc
     1261 ataatcgcta aggtcaaagc cgttttcaag ttttgtcttg gcgttggctg cgtcagcata
     1321 gagacgaacg gcggtattca tcacctggtt gtggtcttcg cgagtctgct ttcctatcac
     1381 gagttgtttg agtcgtgaaa gagagcggaa agggtcgatg atcactttac ccgtgtctgt
     1441 atccatacgg aggaaaagct gtccttcggt gatatatccg gtattatccg gtatggcatg
     1501 cgtgatgtca ccgtcattta gcgtagtcac tgcaatgatg gtgatagagc ctccatccgg
     1561 caattgtaca gccttctcgt agatctttgc gaggtctgag tagagcgatc ctggcataga
     1621 gtccttggaa gggatttgat ccatacggtt ggatacaatg gttaatgcgt cggcatatag
     1681 cgtcatgtct gtaagtagta cgagtacttt ttcatttttg tcaaatgcaa aatactctgc
     1741 agccgcaagt gccatatcgg gaatgagcag acgctccaca gggggctgct cagtagtatt
     1801 tacaaaactg atgattttgt taagggctcc ggcgccctca aacatctgtt taaagtagag
     1861 gtagtcatca ttggagagtc ccattcctcc gaggatgatt ttgtctacat ccgcacggag
     1921 agctacctga gccattacct ggttgtaggg ttggtcgggg tcggcaaaga agggtatttt
     1981 ctgtccggaa acaagtgtat tgttgaggtc aatgccggca atacccgtcg gaatcaattg
     2041 tgatggttgt tttctcctaa aaggattaac ggaggggcct ccgatatagc gtctttcgcc
     2101 ctcaactttg ggaccgtcat ctataggttc cccatacgcg ttgaaaaacc tcccggctag
     2161 atcatcgctc acattcagtg ccggggcttc gccgaaaaac aatacttccg cattggtagg
     2221 tatgccttca gtaccttcaa atatctgaag ggtaacaaga ttgcctttga ttttcaccac
     2281 ctgtgcaaga cgacctgcta caatggcaag ctcgtcattg gtaacacc
//
LOCUS       DAOSIE010000237         2307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_40176_length_2307_cov_4.287300, whole genome shotgun sequence.
ACCESSION   DAOSIE010000237 DAOSIE010000000
VERSION     DAOSIE010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40176_length_2307_cov_4.287300"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLI69_06435"
     CDS             <1..343
                     /locus_tag="PLI69_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG42156.1"
                     /translation="EGCSRYTILWFYLISCEECSKEAVALAEKEKYLRKKGVKVKAIY
                     VGEDEEAWRDFQKRNPKKWVYLWDKTGKSGLDTLYDVRTVPQIYLLDRKKRVIGRELG
                     TEHLFDLLDTL"
     gene            340..>2307
                     /locus_tag="PLI69_06440"
     CDS             340..>2307
                     /locus_tag="PLI69_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HOG42157.1"
                     /translation="MISGKYKEFYDKLKGIIPVQRMHHDPLNTLAFGTDASFYRLIPK
                     LVIKAHSEEEIVAILTTAYNMGLPVTFRAAGTSLSGQAISDSILVLATHGWKEHKILD
                     HGLKICLQPGIRGAAANRYLAPYGRKIGPDPASIDAAMIGGIAANNASGMCCGVADNS
                     YKTVADIRVILPDGTLLDTADEASVRSFKESHKELLSSLERISSDIRSNTELKERIIR
                     KYKIKNTNGYSLNAFTDYSEGIDIFKHLIIGSEGTLAFISSITYNTVIEHPHKALAMV
                     VFTSIKDACTAAHILKEKKATAVELIDRTGVRSADQTPGIPEYLKTIGEESCVLLIEA
                     RGASAEELKANVESLRASISHLPTELPYTFTTDAKEQAMLWKIRKELFPTVAGLRKSG
                     TTPIIEDICFPIEKLAEATVKLREIFERNGYADAVIFGHAMDGNLHFVFNQNFSKDEE
                     VEKYRLFMNDITDMVVSQYDGSLKAEHGTGRNMAPFMEKEWGQEAYSLMKEVKEIFDP
                     KGILNPGVIINSDPKAHISNLKPIPTTREVVDKCMECGFCEGHCVSEGLTLSPRQRVA
                     VFREIEHLKRTGEEPHRAAEMQKLFRYAGEQTCATDSLCHLNCPVKADTGKLIKEIRH
                     ETHSPKGEKRAVMLAGNMDKLTSLLRGGLKLL"
BASE COUNT          701 a          545 c          540 g          521 t
ORIGIN      
        1 tgagggctgc tcacgataca ctatcctatg gttttatctt atttcctgtg aagagtgcag
       61 taaagaggcg gttgctctgg ctgaaaagga aaaatatctc cgaaaaaagg gagtaaaggt
      121 aaaagctatt tacgttggag aagatgagga agcctggcga gattttcaga agagaaaccc
      181 gaaaaaatgg gtatatttgt gggataaaac cggaaaatcc ggactagata cactttatga
      241 tgttcgcacg gtgcctcaga tttacctgct cgatagaaaa aaacgagtca taggcagaga
      301 attgggtacg gaacatcttt tcgatttatt ggacacatta tgatatcagg caagtacaaa
      361 gagttctatg acaaactcaa gggtatcatt cccgtgcaga ggatgcacca tgatcccctc
      421 aacaccctgg cattcggcac ggatgcctca ttctacagac tcattcccaa actggtaata
      481 aaggcacata gcgaagagga aatagtcgct atcctcacta cggcctacaa tatgggactt
      541 ccggtgacat tcagggccgc aggcacctct ctctccggac aggctatcag cgactcgatc
      601 ctcgtcttgg caactcacgg atggaaagag cataagatcc tggaccacgg acttaaaata
      661 tgcctccaac ccggtatcag aggggctgcc gccaaccgct accttgctcc ttacggacgt
      721 aaaataggtc ctgatcccgc ctccatagat gctgcaatga taggcggaat agcagctaac
      781 aatgccagtg gaatgtgctg tggagttgca gacaactctt ataagaccgt agccgatatc
      841 cgtgtcatac ttcccgacgg tactctcctc gatacagccg atgaagcatc cgtaagaagt
      901 tttaaagaga gtcataagga gctgcttagc tctctggaga ggatctcctc ggatataagg
      961 tccaataccg agctcaaaga gcgcattatc aggaaataca agataaagaa taccaacgga
     1021 tactcgctaa acgcatttac ggattacagc gaaggaatcg atatcttcaa gcatcttata
     1081 ataggatcgg aaggcacttt ggcctttatc tcctccataa cctacaatac tgtcatcgaa
     1141 catccgcaca aggcccttgc catggtggta ttcacctcca tcaaggacgc ctgtactgcc
     1201 gcacatattc tcaaggaaaa gaaggcaacg gcggttgaac tcatagacag aaccggtgtg
     1261 cgttctgcag accaaacacc cggaatcccg gaatacctca agacgatagg agaagagagt
     1321 tgcgtgctgt tgattgaggc tagaggagca tctgctgagg aactcaaggc caatgtggag
     1381 tcgctgagag cttccattag tcacctccct acagagcttc cctacacatt taccaccgat
     1441 gccaaagaac aggcaatgct ttggaaaatc cgcaaagagc tcttcccgac agtagcagga
     1501 ttgagaaaat cgggtaccac tcccattata gaggatattt gtttccctat tgaaaaactt
     1561 gccgaagcca cagtaaaact tagagagatt tttgagcgta atggttatgc cgacgctgta
     1621 atatttggcc atgccatgga tggcaatctc cacttcgtat tcaaccaaaa tttttcaaag
     1681 gatgaagagg tagaaaagta tcgcctcttt atgaacgata ttaccgacat ggtagtatca
     1741 caatatgacg gatcactcaa ggccgaacat ggtaccggac gcaatatggc tccatttatg
     1801 gaaaaggaat ggggacaaga agcttatagt ctgatgaaag aggtcaagga gatctttgac
     1861 cccaaaggca ttctaaatcc cggagtcatc ataaattccg atcccaaggc ccatataagc
     1921 aatctcaagc ccattcccac gacacgagag gtggttgaca aatgcatgga gtgtggtttc
     1981 tgcgaaggcc actgtgtctc agaaggattg acactctcgc cacgccagag ggttgccgtc
     2041 ttccgtgaaa tcgaacatct caagagaacg ggagaagaac cccaccgtgc agccgaaatg
     2101 cagaaactct tccgctatgc gggcgaacag acctgcgcta cagactctct ttgccacctt
     2161 aattgtcctg taaaagccga caccggtaaa ctcataaagg agataagaca cgaaacccat
     2221 tcacccaaag gagaaaaaag agcggtgatg cttgcaggaa atatggataa gcttacctca
     2281 cttttgcgtg gcggactgaa gttgctc
//
LOCUS       DAOSIE010000238         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_40546_length_2292_cov_3.623156, whole genome shotgun sequence.
ACCESSION   DAOSIE010000238 DAOSIE010000000
VERSION     DAOSIE010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40546_length_2292_cov_3.623156"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1897
                     /gene="nifJ"
                     /locus_tag="PLI69_06445"
     CDS             <1..1897
                     /gene="nifJ"
                     /locus_tag="PLI69_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOG42158.1"
                     /translation="TKIAAEIGLGGRTNTILQSAFFKITGIIPYEEAVDHMKRAIDES
                     YGTKGENIVKMNYAAVDRGGEYVEVEIPKEWNNATGRFVHANYNRLAPEWIRNVADIV
                     NSQDGNSLPVSAFAGEMADGTIPSGTAAFEKRGIAVTVPEWQPNNCIQCNQCAYVCPH
                     AVIRPFLMTEEEAKAAPAGIKKVQGNGPLKEYKFTIQISPYDCTGCGNCADVCPAKEK
                     ALVMKSFDTQEAEQANFDYLHHNIGYKDNVVPKEQNVKNSQFSQPLFEFSGACAGCGE
                     TPYIKVITQLFGDHMMIANATGCSSIYGASFPSSPYTTNAHGHGPSWANSLFEDNAEF
                     GLGMRLGSERVRDTVANLMKEGLECNSCTSEMKAQFDKWLTNRSNAAVTREIYNELVP
                     MMKECGCNICKQLLELQNFIPARSQWIFGGDGWAYDIGYGGLDHVLASGENVNVLVLD
                     TELYSNTGGQSSKATPAGAVAKFATSGKRVRKKDLGMMAISYGYVYVAQVAMGASQTQ
                     FFNAIKEAEAYDGPSLIIAYSPCINHGLKAGMGLSQREEKMAVECGYWHLYRYNPTFE
                     AEGKNPFSLDSKEPDWSKFQDFLKGEVRFSSLMATFPESAAELFTKTEEFAKWRYNTY
                     KRLAKQE"
     assembly_gap    1927..1936
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2085..>2292
                     /locus_tag="PLI69_06450"
     CDS             2085..>2292
                     /locus_tag="PLI69_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42159.1"
                     /translation="MTSSKRTVLLSAFALLSLFFSLEVYGQQYTISGYVFNSRSHETL
                     LGATIYDLNSKKWTTTNEYGFYTIT"
BASE COUNT          611 a          521 c          558 g          592 t
ORIGIN      
        1 cacaaagatt gctgccgaga tcggtctcgg tggaaggaca aataccatcc tccagtctgc
       61 attctttaag attaccggaa ttatacctta tgaagaggca gttgaccaca tgaagagggc
      121 tatcgatgag tcttacggta ccaagggcga gaacatcgtt aagatgaact acgctgctgt
      181 tgaccgcgga ggagaatatg tcgaagttga gattcctaag gagtggaaca atgccaccgg
      241 tcgtttcgta cacgccaact acaaccgcct tgcccctgaa tggatccgca atgtggccga
      301 catagttaat tcccaggatg gaaattctct tcctgtcagc gcattcgccg gagagatggc
      361 tgacggcacg attcccagtg gaaccgctgc ttttgaaaag cgcggtattg ccgttaccgt
      421 tcccgagtgg caacctaaca actgtatcca gtgtaaccaa tgtgcttacg tttgtcctca
      481 tgctgtaatt cgtcccttcc tgatgactga agaggaggcc aaagctgctc ccgccggcat
      541 aaagaaagta cagggcaatg gacctttaaa ggagtacaaa ttcacaatac agatttctcc
      601 ttatgactgt acaggttgtg gcaactgcgc agatgtctgt ccggccaagg aaaaggctct
      661 tgttatgaaa tctttcgata ctcaggaggc agagcaggct aacttcgatt atctgcacca
      721 caatatcggt tataaggata atgttgttcc caaggagcaa aatgttaaga attctcagtt
      781 ttctcagcct ctcttcgagt tctccggagc ttgtgcaggt tgtggtgaga ccccttacat
      841 caaagtcatt acacagctct ttggtgacca catgatgatt gcaaatgcta caggttgttc
      901 atcaatctac ggagcatcat tcccttcatc accatacact accaacgctc acggacatgg
      961 tccttcatgg gctaattccc tcttcgagga caatgccgag ttcggcctcg gtatgaggtt
     1021 gggtagcgag agagttcgcg atactgtcgc aaatcttatg aaggaaggtt tggagtgcaa
     1081 cagctgtact tcggagatga aagcccagtt cgacaagtgg ctgaccaatc gtagcaatgc
     1141 agcagttact cgtgagattt acaatgagtt ggtgcctatg atgaaagagt gcggatgcaa
     1201 tatctgcaaa caactcctcg aactgcaaaa cttcattcct gcccgttcac agtggatctt
     1261 tggaggagac ggttgggcat acgacatcgg ctacggcgga cttgaccacg ttctggcctc
     1321 tggtgagaat gtaaatgttc tcgtactcga tactgagctc tattcaaata ccggcggaca
     1381 gtcatccaag gcaactcctg caggtgctgt tgctaaattt gccacttccg gaaagagggt
     1441 tcgcaagaaa gatctcggaa tgatggcaat cagctatggt tatgtctatg tggcacaagt
     1501 agcaatggga gcaagccaga ctcagttctt caatgctatc aaggaggcag aggcctatga
     1561 tggcccttca ctcatcattg cttattcacc ctgtatcaac cacggtctca aggccggtat
     1621 gggcttgagc cagcgggagg agaagatggc agttgagtgt ggttactggc atctgtatcg
     1681 ctacaatcct acttttgagg cagaaggcaa gaatcccttc tcgctcgata gcaaggagcc
     1741 ggattggagc aaattccagg atttcctcaa gggtgaggta cgcttctctt cactaatggc
     1801 tacattccct gagagtgcag cagagctctt caccaagact gaggagtttg ccaagtggcg
     1861 ttataacacc tataagagac ttgctaagca agagtaattc ttatttgtgg attaggatgc
     1921 tcccgtnnnn nnnnnnatgg atagaatgga agattccggt tcttccggtt ggggttcgat
     1981 tccccacggg ggtaccatcc tgcaaaataa aaatgtgcca actaaaaaaa tagttggcgc
     2041 attttttgca taaagtgtgc cggaaaagaa aaatcacaat cgatatgacg agttctaaaa
     2101 gaacagtttt actctctgct ttcgctcttc tgagtctctt tttcagttta gaggtgtacg
     2161 gacagcagta caccattagc ggctatgttt ttaattcccg cagccacgag actttgcttg
     2221 gagctacaat ttatgatctg aatagcaaaa agtggacaac cacaaatgag tatggttttt
     2281 acactatcac ac
//
LOCUS       DAOSIE010000239         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_40609_length_2289_cov_3.851835, whole genome shotgun sequence.
ACCESSION   DAOSIE010000239 DAOSIE010000000
VERSION     DAOSIE010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40609_length_2289_cov_3.851835"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..554
                     /gene="folB"
                     /locus_tag="PLI69_06455"
     CDS             201..554
                     /gene="folB"
                     /locus_tag="PLI69_06455"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOG42160.1"
                     /translation="MEAKLKLENMEFYAYHGCLEHERKVGNRFRVDFECTYRAMAAAL
                     SDNLNDAPDYSQIYAFIEREMALPSNLLENVAYRILNVVRNEFPAITSASVTVAKYNP
                     PLGGQVYTSSITLSF"
     gene            560..1888
                     /gene="mtaB"
                     /locus_tag="PLI69_06460"
     CDS             560..1888
                     /gene="mtaB"
                     /locus_tag="PLI69_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOG42161.1"
                     /translation="MESSPGYTVAFATLGCKLNFAESSAIASSFKEAGYRQVSANSPA
                     DIYVVNTCSVTEHSDKKCRNLIRKLHKLSPAALIGVTGCYAQLKPKEVLQIEGVAVLF
                     GNGHKEEVFETLHRMLQERKPDREQKPEEERENYKKLSLISPIEGIKSIFPAYSSGER
                     TRSFLKVQDGCDYHCSYCTVPLARGGSRNIPISTLVSQAESIASSGIVEIVLTGVNTG
                     DFGKSTGESFLELLKQLNRVGGITRYRISSIEPNLLTEEIVEWIASGTKFLPHFHIPI
                     QSGCDAVLKRMRRRYTTDLFASRIEMIRDKMESPEQRFSRVFFGIDVIVGFPGESDEE
                     FDQTYRFLEKIRPAFLHIFPYSRRAGTPAAAMPDQVPENVKRVRVARLKELSDRLNEE
                     FCAANKGRTLPVLFESTRKGGMMLGYTDNYIKVERPYDPALIGRISQVTL"
     gene            complement(1909..>2289)
                     /locus_tag="PLI69_06465"
     CDS             complement(1909..>2289)
                     /locus_tag="PLI69_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOG42162.1"
                     /translation="YLRYDEIGAEVVANVRAHEKEMVEKIASADEQALLLHKKSPRRA
                     VKYLTAFSTNEADALFEKWNELDKYLLVKYIDGNTKRQDETGAFITNGHSATIPPGPI
                     QKGYNERFKRAIVKEIGEKIKVVK"
BASE COUNT          600 a          525 c          552 g          612 t
ORIGIN      
        1 attgaaactc cctatcgcaa cgatgctttg atggaagatc tgctcgcgtg ctgtaaagat
       61 agtaccacta tctgcgtcgc agccgacctg accctgcctc agtagtacat ccgtacacgt
      121 accgtagcgg attggaaaaa ttctccgata actatcggga aacgcccctg tgtatttgta
      181 attcaggcat aaatagaatt atggaagcaa aattgaaact tgaaaatatg gagttttatg
      241 cctaccacgg ctgtttagag cacgagcgta aggtgggcaa ccgctttaga gtggactttg
      301 agtgcaccta tcgggcaatg gctgccgctc tctcggacaa tctgaatgat gcgccggact
      361 attctcaaat atacgccttt atcgaacgcg agatggcgct cccatccaat ttacttgaaa
      421 atgtagctta tcgcatcctg aatgttgtgc gcaacgagtt tcctgcaatc acaagcgcaa
      481 gcgtaaccgt agccaaatac aatcctccgc taggaggcca ggtctataca tcttccatta
      541 ccctgagttt ctgatactga tggagagctc ccccggatat acggtcgcat ttgcaaccct
      601 cggatgcaaa ctcaactttg ccgagagctc cgctattgcg agctctttta aagaggccgg
      661 ataccgtcag gttagcgcca atagccctgc tgatatctat gtggtcaata cctgttcggt
      721 aaccgaacac tcagataaaa agtgccgtaa tctcattcgc aaacttcata aactctctcc
      781 tgctgctctg atcggggtta caggttgtta tgcacagttg aagcctaagg aggtgctgca
      841 aattgaggga gttgcagtgc tcttcggaaa tggccacaaa gaagaggttt ttgaaacact
      901 tcaccgtatg ctacaagagc gcaaaccgga tagagagcag aagccggaag aggagcggga
      961 aaactataaa aaactctccc taatttcccc gatagaggga ataaaatcca tttttcctgc
     1021 atactcatcg ggggagagga cccgctcttt tctaaaggta caggatggtt gtgattatca
     1081 ctgctcctat tgtacagtgc ctcttgcgag gggtggaagt cgcaatatac caatttccac
     1141 cctggtctca caagcagaat cgatagcctc atccggcata gtggagattg ttttgaccgg
     1201 ggtaaataca ggtgatttcg ggaaaagcac aggagaatct tttctggaat tgctaaagca
     1261 actcaataga gtcgggggca tcaccagata taggatttcc tccatcgaac ctaatctcct
     1321 tacggaagag atcgtggagt ggatagcttc cggcactaaa ttcctgccgc atttccatat
     1381 tcccatccag tcgggatgcg atgccgtact caaaaggatg cgtcgacgtt acacgacaga
     1441 tcttttcgcc tcccgcatag agatgataag ggataagatg gaatcacctg agcagcgttt
     1501 cagtagagta ttcttcggga ttgatgtgat tgtgggtttt ccgggagaga gtgatgaaga
     1561 atttgatcaa acctatagat ttttggagaa aatcaggccg gcatttctgc atatatttcc
     1621 ctattcgaga agggccggaa ctccggctgc ggctatgccg gatcaggtac ccgaaaatgt
     1681 gaaaagagta cgtgtagcac gacttaaaga gctttccgat aggttaaatg aggaattttg
     1741 tgctgcaaat aaaggtcgaa cgctgcccgt gctttttgaa agcacccgaa aggggggtat
     1801 gatgctgggg tataccgaca attatataaa agtagagcgt ccctacgatc cggcgcttat
     1861 cggcaggatt tcgcaggtaa cgctctaaat ctctaccttt cggtaaacct atttcacaac
     1921 ctttatcttt tccccgatct ctttgactat agcacgtttg aagcgctcat tgtagccctt
     1981 ttgaataggt cccgggggaa ttgttgccga gtgcccgttg gtgatgaagg cccctgtttc
     2041 atcctggcgt ttggtgtttc cgtctatata ttttacgagg aggtatttat ccaattcgtt
     2101 ccatttctca aatagtgcgt cggcttcatt tgtagagaat gccgtgagat attttaccgc
     2161 tctgcgaggg gattttttat gcaggagaag agcttgttca tccgcagagg cgattttttc
     2221 caccatttcc ttttcgtgag cacggacatt ggcgacaacc tcagcaccga tttcgtcata
     2281 gcgcaaata
//
LOCUS       DAOSIE010000240         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_40891_length_2277_cov_3.962196, whole genome shotgun sequence.
ACCESSION   DAOSIE010000240 DAOSIE010000000
VERSION     DAOSIE010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40891_length_2277_cov_3.962196"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLI69_06470"
     CDS             <1..368
                     /locus_tag="PLI69_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42163.1"
                     /translation="IFIHLGKKPQWLEWNVFTLYSQYISTKNFALIRNNQGDELSVLL
                     YFIGAFMLIFSAERKEKSGYNVARLRAFYKTVVISMVIFVAGYMLLHGMAIIAFAAAM
                     PFVVPLIYLALFYGSLRRY"
     gene            373..1296
                     /locus_tag="PLI69_06475"
     CDS             373..1296
                     /locus_tag="PLI69_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42164.1"
                     /translation="MEIAPFEMHEIILYVIAPPYPINWQGPADLLNSAIKSFSKMIFS
                     FQYRFIGHTIVVLKSSHIEETKAYSISGAKPIEVFKGVFKEGIGLGILGGIFKAYIES
                     PHKIESTLRFNSKKGKVLFIKYRINAASAKRIIDFFERFEHKFDNHFAPMDFYGGALW
                     PLYENEGAACSAFCVSTLIAGNLINEEAEEWKVNFKIPIKLIGGRYNKGNKVSVPDVL
                     RTKEWYHEEGIEGVDFISLEIYDPNKMFNWIKKTLEINDNYFKPVTENGISGLLVDCR
                     DLDIEGETAPITKRPAISSFISEYYSQAGLM"
     gene            complement(1598..2062)
                     /locus_tag="PLI69_06480"
     CDS             complement(1598..2062)
                     /locus_tag="PLI69_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42165.1"
                     /translation="MNKTILNEHNKEDHPPAHTAEHILNGTMVKMFGCGRSYNAHVER
                     KKSKCDYRLEKEPTPEEIALIEKRVNEVIASNLDVTESFLTQEEAASIVDLSKLPDDA
                     SEMLRIVRVGDYDACACIGAHVANTSEIGTFKIISSDFEDGRWRLRWRIVKE"
     gene            complement(2065..>2277)
                     /locus_tag="PLI69_06485"
     CDS             complement(2065..>2277)
                     /locus_tag="PLI69_06485"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294924.1"
                     /note="catalyzes the release of the N-terminal amino acid
                     from a tripeptide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOG42166.1"
                     /translation="KAMELAGIKPNIKPIRGGTDGARLSFMGLPCPNLFAGGHNFHGK
                     LEFVPVESMEKAVQVILNLISLYAAE"
BASE COUNT          654 a          448 c          480 g          695 t
ORIGIN      
        1 atatttttat tcatttgggt aaaaagcctc aatggctgga gtggaatgtt tttacactct
       61 attcacaata catatcaacg aagaattttg ctctcataag gaataaccag ggcgacgaat
      121 tgtcggtgtt gctatatttt ataggggctt ttatgttaat tttttctgcc gagaggaagg
      181 aaaaaagtgg ctacaatgtt gcccgtctcc gtgctttcta taaaacagtt gtaatatcaa
      241 tggtgatttt tgtcgccgga tatatgttgt tgcatggtat ggcaatcatt gcgtttgccg
      301 cagcaatgcc ttttgttgta ccactcatct atttagctct cttctatggg tcacttagga
      361 ggtactgagt gattggaaat agccccattt gaaatgcatg agattatctt atatgtaatt
      421 gcgccaccct atccgataaa ttggcaaggt cctgctgatt tattaaactc tgctataaaa
      481 tccttttcaa agatgatttt tagctttcaa tatcgtttca taggacatac tattgttgtc
      541 ctgaaaagca gtcatattga agaaacaaaa gcgtatagta tatcgggagc caaaccaata
      601 gaggtcttta aaggcgtttt taaggaaggg attggccttg ggatactcgg aggtattttt
      661 aaggcttaca ttgaatcgcc gcataaaatc gaaagtactc tgaggttcaa ttcaaaaaaa
      721 ggtaaggtac tttttatcaa atatcgtata aatgcagcat cagcgaaaag gataattgat
      781 ttctttgaaa gatttgaaca taagttcgat aatcactttg cgcctatgga tttttacgga
      841 ggtgctttat ggccattgta cgagaatgaa ggggcagcct gttccgcttt ttgtgtctct
      901 accctgattg ccggcaatct tattaacgag gaagctgaag agtggaaagt taatttcaaa
      961 attcccatca aactaattgg tggcaggtac aataaaggaa ataaggtatc cgtacctgat
     1021 gttctgagga ctaaggaatg gtaccatgag gaggggatcg agggagttga tttcatttca
     1081 ctggaaattt atgatccaaa taagatgttc aattggatta aaaagacctt ggaaatcaat
     1141 gacaactact tcaaacctgt tacggaaaac ggtatctccg gactcctggt tgattgtagg
     1201 gacttagata tagaaggaga aacagcacct attacaaaaa gacccgctat atcatcgttc
     1261 atttccgaat attatagtca agcaggttta atgtaataag cttgattgcg ctatattctt
     1321 ggtttttgaa ttatactaag tgctttttcc cagttctatt ggaaaaggag cgaaatgaat
     1381 tattgtacag aaattctgac gagtttttag aaaatttaca tttattctga cgactaaatg
     1441 aggtttttgc acagaaattc tgacgagtcc aaaaatattg acacaaaaaa gcgacccgaa
     1501 ggtcgctctt aattactcag aaatcgatac taataactcg gaactaaccc accctgcacc
     1561 cttccgcaac tacacactct atactaccca ctacaaacta ttcctttact attcgccagc
     1621 gtaagcgcca gcgcccatct tcaaaatccg aagaaataat cttgaatgtg cctatttcgc
     1681 tagtgttggc cacgtgtgca ccgatgcaag cacaagcgtc ataatcgcct acgcgcacaa
     1741 tgcgtagcat ttcagaggca tcgtctggta gtttgctcag atccacgatt gatgctgcct
     1801 cttcctgcgt aagaaaactc tcagtcacat ccagattgga cgctatcacc tcgttcactc
     1861 tcttttcgat cagggcgatc tcttcaggag taggctcttt ttcaagccga taatcgcact
     1921 tactcttttt gcgctcaaca tgggcattat atgaccggcc acaaccgaac atcttcacca
     1981 tagtaccatt aagaatatgc tcggcagtat gcgcaggcgg atgatcttcc ttgttatgtt
     2041 cgttgagtat ggttttgttc atatctactc cgctgcgtaa agtgaaataa ggttgagtat
     2101 cacctgtacg gctttttcca tgctttcgac agggacaaat tcgagtttgc cgtggaagtt
     2161 gtggccgccg gcaaagagat tggggcaggg gagtcccata aatgagagtc ttgcgccgtc
     2221 agtacctccg cggataggct tgatattagg tttgatacct gcaagttcca ttgcttt
//
LOCUS       DAOSIE010000241         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_41590_length_2249_cov_3.074749, whole genome shotgun sequence.
ACCESSION   DAOSIE010000241 DAOSIE010000000
VERSION     DAOSIE010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41590_length_2249_cov_3.074749"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PLI69_06490"
     CDS             complement(<1..515)
                     /locus_tag="PLI69_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42167.1"
                     /translation="MEEDKTISFILEHAEEESVQLLLSASRYPGVDMNRAASAIEARK
                     KIVYKVPLWHTYPELIYPTSLSVEQCSSQTTAEYKMRFVPEAGTMADLTGGLGVDCSF
                     MALRAEKCLYMERDPRLYEAAQHNFKVLGLENITVRNGDCREYLKRFTEPNEELTAIN
                     KELEGANEELS"
     gene            620..>2249
                     /locus_tag="PLI69_06495"
     CDS             620..>2249
                     /locus_tag="PLI69_06495"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOG42168.1"
                     /translation="METNYLEVAKSWLGVGFDDETKKEVARLMREDPKELEDCFYRLL
                     EFGTGGLRGLMGVGTNRMNKYTVGMATQGMANYLKSYFKDSGKISVVISYDSRNNSRE
                     FAMIASRVLMANGINVFLFDNIRPTPELSFAIRHLECQAGIMITASHNPKEYNGYKVF
                     WEDGAQIIASHDKNIIAEVAGITSVAQVNFGDDLALFPTPIGEEVDKAYLDAVLSLTL
                     SPDSVKRHSDLKIVYTPLHGTGVRLVPQGLRQIGFSNIYNVNSQDVSDGNFPTVTSPN
                     PEDPAALKLAIEKALEVGADIVMATDPDADRVGFAVRDDKGELVLLNGNQIFSILTSY
                     MLTRWSELGKLQGGEYVVKTIVTTELVRAICEKYGVELYNVLTGFKYIAEIVRKNEGK
                     KVFICGGEESHGFNAGEFVRDKDSIVSCALFAECAAWLADSGKSIYDYLQEIYSEYGY
                     YKEKLLSVTKSGKEGLEQMPVKTNFKDNKKFLAAQELYLKTQGWIKTIHKLKDLKQHP
                     EKPFNHFDWKLDFPEVLNPYLVPDENQRGFDIVIG"
BASE COUNT          641 a          461 c          511 g          636 t
ORIGIN      
        1 tccgacaatt cttcatttgc cccctccaac tctttattta ttgctgttaa ctcttcatta
       61 ggctccgtaa atctcttcag atactctctg caatcaccgt ttcttacagt aatattttca
      121 agaccgagca ctttaaaatt gtgttgtgcc gcttcataca atcgtgggtc gcgctccata
      181 taaaggcatt tctccgctct gagagccata aaactgcaat ccactccgag tcctcccgta
      241 agatcggcca tagtgccggc ttcaggcacg aaacgcattt tatattccgc cgtagtctgg
      301 gaactgcatt gttccacgct gagagaggtg gggtaaataa gttcaggata ggtgtgccat
      361 agaggtacct tgtagacaat tttcttgcgc gcctctattg cagatgccgc tctgttcata
      421 tccactccgg gatagcgcga ggcgctaaga agcagttgaa cactctcttc ctctgcatgt
      481 tccaaaatga atgatatggt tttatcttct tccaaagggt tttagtctct catagggggc
      541 aaagatagct tattaatcaa cttttttatg taagtttgtg tgttttatta gctataattt
      601 ataatactat cagatcaata tggaaacgaa ttacctggaa gttgcaaaga gttggctagg
      661 cgtcggattt gacgatgaga caaaaaaaga agttgcgcgc ctcatgaggg aggatccaaa
      721 agagctcgag gattgttttt atcgcctgct ggaattcggt acaggaggat tgaggggcct
      781 tatgggtgtt ggtacgaacc gtatgaataa atatacggtt ggcatggcca cacagggcat
      841 ggcaaactat ctcaaatcat attttaaaga ctccggcaaa atcagtgtcg tgatttcata
      901 cgacagtcgc aataacagtc gagagtttgc tatgattgcc tccagagtgc ttatggccaa
      961 tggtataaat gtttttcttt ttgacaatat aagacccact cccgaattga gttttgccat
     1021 ccgacacctc gaatgtcagg caggcataat gattaccgct tcccacaatc cgaaggaata
     1081 taatggttac aaggtattct gggaggatgg agcacagatt atagcatcac atgacaagaa
     1141 tatcatagca gaggtggccg gcataacttc ggttgcacag gtgaattttg gtgacgatct
     1201 tgcacttttc cccacaccta taggggaaga ggtggacaaa gcctatcttg atgcagtact
     1261 ttctctcaca ctttctcccg attccgtaaa gcgccattcg gatttaaaaa tagtctatac
     1321 acctttacat ggcactggag tacgtcttgt tccccaaggg ttacgacaga taggattttc
     1381 aaacatttat aacgttaatt cacaggatgt cagtgacgga aattttccca cagttacttc
     1441 gcccaatccc gaagatcctg ccgcacttaa acttgcgatt gagaaagccc tggaagtggg
     1501 cgcagatata gtgatggcaa ctgaccctga tgcagaccgt gtaggatttg ctgtacgaga
     1561 tgacaaaggg gagttggttt tgttaaatgg caaccagata ttctctattc tcacaagcta
     1621 tatgcttacc cgttggtctg agctgggtaa actccaggga ggtgaatatg tggtcaagac
     1681 catcgttacc accgagctcg tccgtgctat ttgtgaaaaa tatggagttg aactctataa
     1741 tgtgcttacc ggatttaaat atatagctga aattgtgcga aaaaatgagg gtaaaaaggt
     1801 tttcatttgt ggtggtgagg agagtcacgg ctttaatgcc ggtgaatttg taagagataa
     1861 ggactccata gtttcatgtg ctctctttgc ggagtgtgct gcatggctgg ccgatagcgg
     1921 taagagtatc tacgactatc ttcaggagat ttattccgag tatggttatt acaaggagaa
     1981 gctcctttcc gttacaaaga gcggaaaaga gggactggaa cagatgcctg taaaaacaaa
     2041 ttttaaagac aataaaaaat ttttggcagc tcaggaattg tatttaaaaa ctcaaggctg
     2101 gataaaaaca atacacaaac tgaaagacct aaaacaacac cccgaaaaac cttttaacca
     2161 tttcgactgg aaactcgatt ttccggaggt gctaaaccca tacctggtgc ccgatgaaaa
     2221 ccaacgaggg tttgatattg taattggga
//
LOCUS       DAOSIE010000242         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_41690_length_2245_cov_3.631963, whole genome shotgun sequence.
ACCESSION   DAOSIE010000242 DAOSIE010000000
VERSION     DAOSIE010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41690_length_2245_cov_3.631963"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2245
                     /locus_tag="PLI69_06500"
     CDS             <1..>2245
                     /locus_tag="PLI69_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOG42169.1"
                     /translation="AKYSDHPRVLRLHYIKLEYERYKIADKDNQSGADYLAFKARCLE
                     VLKGHSSKAYYYKNIEQMIEEMESEDLSASIVSHKSQYIPHQDMTIEIGTRNISRVTL
                     DIYRLDDNRFWESGYKLDMFNLRRISKKVFSRTYDMPNEYNISSERRDTISCGAAGNY
                     ILHFHSGKHNSYSALSVSSVATGLRKVGNTQEIYAADIVTGKPYKEVLAEVYRDSDIK
                     SRFGVTKLTPTKKAVVTQKGFTSLPLKLEKKKGNESIIWRLRVSAGKDIYAPFLSIDN
                     YYEEIDESTYMLENMALYTDRKLYKPSDTIFFKVICYKSDRERGEVLGNKPIELSIRH
                     TSEDKPFATFKVVTNDMGSASGFFKIPEDGKNGIYSIIAGNRYLGEVRVESYKRPNFD
                     FRLEPNEGVYIFSDIVRQKGTVESFAGYSIAGSKVEYTVTRRSEWYPGRKSYDYYYSE
                     EVASGEVLSDNDGRFEISFVAEHPVGESFNPKLHSENVIAFVINAKVTDPQGETHSKS
                     TTVRVSDIPLVMGLRFPVPTSQSVTDPESGISSVIVEKNEVKAVTFTVDNLNHRPYDT
                     KVTYSLLQDGKVVRSETVSSNSEVPFDFASVKSGKYTLAYSTVYRGIKVEGKTDIVIF
                     DINEKRLPFKAKYFYYPIVTENGIDFLLGTTEDDLYLEAGLFDNHKCHHRESLHLKNE
                     VVRVTLPYKEEYRSSIKLSVFGFRNGEPIDHQYEFSRPGKVLFNVDIESFRDCTGPQT
                     KEKFTITG"
BASE COUNT          684 a          467 c          508 g          586 t
ORIGIN      
        1 gccaagtatt cagaccatcc aagagttttg aggctccatt atattaagct tgagtatgaa
       61 agatataaga tagccgacaa agataatcag agcggtgccg actacctggc ctttaaagcc
      121 cgttgtctgg aagtactaaa aggtcactcc tccaaagcct attattataa aaatatagaa
      181 caaatgatag aagagatgga gtcggaggat ttatcggcaa gtattgtttc acacaagtca
      241 caatatatcc ctcaccagga tatgaccatc gagataggaa cacgcaatat tagtcgcgtg
      301 accctcgata tataccgact tgatgataat cgtttctggg aaagtggcta taaactcgac
      361 atgttcaatc tcagacgcat atctaaaaag gttttttccc gcacctacga tatgccgaat
      421 gagtacaata tttcatcgga acggagagat accatttcct gtggggcggc aggcaattat
      481 attctccatt tccattcagg gaaacacaat tcttatagcg cccttagcgt aagttcggtg
      541 gctacggggc ttcgcaaggt gggcaacaca caggaaatct atgcggctga catagttaca
      601 ggcaagccct ataaagaggt tctggcagaa gtatacaggg attcggatat caaaagccgt
      661 ttcggtgtga caaagcttac ccctacaaag aaggctgtag tcactcagaa gggatttacc
      721 tcgcttcctc tgaagctcga aaagaaaaaa ggcaatgaat ctataatctg gagacttaga
      781 gtctcggcag gcaaggatat ttacgccccg tttttgtcaa tagataatta ttatgaagaa
      841 attgatgaga gtacatatat gttggagaat atggcactct acactgatcg caagctctac
      901 aagccctccg ataccatttt cttcaaagta atctgctata aaagtgacag agaaaggggt
      961 gaggtcctcg gcaacaagcc tattgagctc tcaatacgcc atacttctga ggataaacca
     1021 tttgccacct ttaaagtggt gacaaacgat atgggatcag ccagcggttt tttcaaaata
     1081 cccgaagatg gtaaaaacgg gatttactct attattgcag gcaacagata ccttggtgag
     1141 gttcgggtcg agagctataa aagacccaat tttgatttca ggctcgagcc gaatgaggga
     1201 gtttacatct tttccgatat agtgcgtcaa aaaggaaccg tggagagttt tgccggctac
     1261 tccattgcgg gaagcaaggt agaatatact gttacacgcc gttccgaatg gtatcccggc
     1321 cgtaaatcct acgattatta ttattcggaa gaggtggctt ccggagaggt actgtcggac
     1381 aatgatggac ggtttgagat ctcttttgtc gcagaacatc ctgtagggga gagttttaat
     1441 cccaagctcc actccgaaaa tgtcattgcc tttgtgatca atgccaaagt taccgacccg
     1501 cagggagaaa cccatagcaa gagcaccacg gtaagggttt ccgatatccc tctggtaatg
     1561 ggactccgtt ttccagtccc gacatcacaa agtgtcacag accctgaaag tggaatttcg
     1621 agtgtgattg tggagaaaaa tgaggtcaag gctgtgacat ttaccgtaga taatcttaat
     1681 catcgtcctt atgatacgaa ggttacttat tcgctgttgc aggatggaaa ggtggtaaga
     1741 agcgaaacgg tatcatcaaa tagtgaagtt ccatttgact ttgcttctgt caagtccgga
     1801 aaatacaccc tcgcttatag caccgtttac aggggtatta aagttgaagg caagacagat
     1861 attgtgatat tcgatataaa tgaaaagcga ctgcctttca aggcgaaata tttttactat
     1921 cctattgtaa ccgaaaacgg catagatttt ctcctcggta cgaccgaaga tgacctctat
     1981 ctcgaagcag gtctctttga taatcataaa tgccaccata gggaatcact gcacctcaaa
     2041 aatgaggtgg tgagagttac gctgccttat aaggaagaat atcgcagttc aataaaactc
     2101 tccgttttcg gatttcgcaa cggagagcca attgaccatc aatacgaatt ttcccgtcct
     2161 ggcaaagttc ttttcaatgt ggatattgaa tccttcagag attgcaccgg ccctcaaaca
     2221 aaggagaaat tcaccattac gggag
//
LOCUS       DAOSIE010000243         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_42028_length_2232_cov_4.691318, whole genome shotgun sequence.
ACCESSION   DAOSIE010000243 DAOSIE010000000
VERSION     DAOSIE010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42028_length_2232_cov_4.691318"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="PLI69_06505"
     CDS             <1..1519
                     /locus_tag="PLI69_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOG42170.1"
                     /translation="EITDPVTLPNGYRYYGERTATSSDNNVKEMVGQACALVDSKVNF
                     ANFDADGDGVVDNVFIIFAGYNEAEGGGDATIWPQSWNVSSLELYFDGKKISNFSCYS
                     EFSGSSGERFAGIGSICHEYCHMLGLLDMYDVNGDTEGYSPLLLGPLSIMDNGNYNNG
                     GKTPPYLTVFEREMLGAVSPSRLHGSTSIEVLPVHQETRAYIIPAYSNGESFYLEYRD
                     GSGWDAHIGGEGLVVYHIDKSYGSACSMSAKMRWNSNAVNACASHPCVRPIASSGNSY
                     TDISMLFFPGKNNVTHIHSSYSFPLLDWSGKGHGYGIANIHKGSAGMLLDIIADNGWD
                     SPVVKEYSIEPQQRTALLKWSTDKEESRGNWNIVWSLENSVYADTSVISVTQRDYLFT
                     DLKPGSTYNCRLFYSRNNVIGKSYPMSFSTREMFSLFPMIADMDKSYKVGDELHLTIL
                     NIAEEVISIRWIVNGAEVPDGKLKFDRSGLFEIKAEISYPDNSVEYIVKKKTISQ"
     gene            1590..1991
                     /locus_tag="PLI69_06510"
     CDS             1590..1991
                     /locus_tag="PLI69_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42171.1"
                     /translation="MAISCPGPEPPPIVVPDPIESFEKKAFKVNSDEGLYVKGACVVR
                     YSEETFQKSFNEKRRTYRMQSDDQISYLHIAFISDLPAEIDQEVICNITYTREKGEET
                     ILIVKFVLAQIKGGKVWLWNELQKTGIILDR"
     gene            complement(2054..2227)
                     /locus_tag="PLI69_06515"
     CDS             complement(2054..2227)
                     /locus_tag="PLI69_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1"
                     /protein_id="HOG42172.1"
                     /translation="MKELLDAIKAEIEVFSKNAEAQLVKGNKAAGTRARKAALNLSKM
                     MKDFRKVSMNASR"
BASE COUNT          618 a          480 c          532 g          602 t
ORIGIN      
        1 tgagattacc gaccccgtca cgcttcccaa cggctacaga tattacggag aacgtacggc
       61 aacgtcctcc gacaataatg taaaggaaat ggtggggcag gcctgcgccc ttgtggatag
      121 caaggtcaat tttgctaatt ttgatgcgga cggtgacgga gttgtcgaca atgtattcat
      181 aatatttgca ggctataacg aagctgaagg gggaggagat gccactatct ggccgcagtc
      241 ctggaatgtc tcctcgttag aactctattt tgacggcaaa aagatatcca atttctcatg
      301 ttattccgaa ttttcaggct cttcaggaga gagatttgca ggaataggct ccatctgcca
      361 tgaatattgt cacatgctgg gtcttttgga tatgtatgat gtcaatggtg acacggaagg
      421 atattcgcca ctccttctcg gtccgctatc aataatggac aacggcaatt acaataacgg
      481 tggtaaaact ccaccctatc tcacggtttt tgaacgcgaa atgctgggtg cggtatctcc
      541 gagccgactt catggtagta caagcattga agtacttccc gttcatcagg aaaccagggc
      601 ctacatcatc cctgcctact ccaatggaga aagtttctac ctggaatacc gggatggttc
      661 aggctgggat gcccatatcg gaggtgaggg tctggtggta tatcatatag acaagtccta
      721 tgggagcgcc tgctccatga gtgccaaaat gcgctggaac agcaatgccg tcaacgcttg
      781 tgcctcgcat ccctgcgtgc gtcccattgc ctcttccggt aatagttaca cggatatatc
      841 catgcttttt ttcccgggta aaaacaacgt cacacatatt cactcctcat actcttttcc
      901 tctgcttgac tggtccggaa aaggtcacgg atatggcata gcaaacatac acaaaggctc
      961 tgcaggaatg ctgctcgaca ttatcgcaga caatgggtgg gactctccgg tagtaaaaga
     1021 atattccatc gaaccgcagc agcgtacagc acttttgaag tggagtactg acaaggagga
     1081 gtcccggggc aattggaaca tagtttggag tttggaaaac agtgtctatg ccgacacctc
     1141 ggttatttcg gtaacgcaaa gagattacct gtttaccgat cttaagccgg gtagtactta
     1201 taattgccgt ctgttttact cacgaaacaa tgtaatagga aagagttatc cgatgagttt
     1261 ttctacgagg gagatgttct ctctctttcc tatgattgca gatatggata aatcatataa
     1321 ggtgggtgac gagcttcatc ttaccattct caacattgca gaggaggtga tctccatccg
     1381 ctggatcgtg aatggggcgg aggtacctga cggaaaatta aaatttgacc ggagcggact
     1441 attcgaaata aaagctgaaa tttcctatcc ggacaattcg gttgagtata ttgtgaagaa
     1501 aaaaactata agtcagtaag atgattttgc acaacaggat acggtcattt gtcacaaggt
     1561 tattactgct gttagttacg gcagtgacta tggccatatc atgtcccggg cctgaacctc
     1621 cacccatagt tgtgccggac cccatagagt cgttcgagaa aaaagccttt aaggtcaata
     1681 gtgacgaggg attatatgtt aagggtgcct gcgttgtccg ttattcggag gagaccttcc
     1741 aaaaatcatt caatgaaaaa agaagaactt accgtatgca gagcgatgat cagattagct
     1801 atctccatat cgccttcata agcgatcttc cggcagagat cgaccaggag gtcatttgta
     1861 atataactta taccagggag aaaggtgaag agactatcct catcgtcaaa tttgttctgg
     1921 cccagattaa gggaggtaag gtttggctct ggaacgaatt gcagaagaca ggtataattc
     1981 tggataggta aagtagtaaa aaataaaagg ggtatccgaa cggacacccc ttactctttc
     2041 gtacaaccaa aggttacctg gaagcgttca tagaaacctt tctgaagtct ttcatcattt
     2101 tgctcaagtt caaagcagct tttcttgcac gtgtgccggc agctttgttg cccttaacaa
     2161 gctgagcttc agcgttcttg gagaaaactt caatttcagc tttgattgcg tcaagtagtt
     2221 ctttcatata at
//
LOCUS       DAOSIE010000244         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_42562_length_2211_cov_4.126623, whole genome shotgun sequence.
ACCESSION   DAOSIE010000244 DAOSIE010000000
VERSION     DAOSIE010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42562_length_2211_cov_4.126623"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLI69_06520"
     CDS             <1..457
                     /locus_tag="PLI69_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOG42173.1"
                     /translation="ANVQTFYGTNITPQRGALNENIWATLETTVRDWSKNLDTLYVVT
                     GCVIEGSTEFVNDNEGKRVTVPTGYYKALLGYKQNASIGGSTKGFVGIAFYFDHKGYS
                     NDYTTMMKQSMTISALEKKVGVDFFVNLPAKIGADLAQKVETGIDPFWK"
     gene            480..1283
                     /locus_tag="PLI69_06525"
     CDS             480..1283
                     /locus_tag="PLI69_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOG42174.1"
                     /translation="MKRNLKWIAYTVVAVMIAVACSSVAFTGRKRVLLYSDSQISSLS
                     EQSYQEFMTKAKLSTNKQMQATVQEVGRKMTTALEKYLTSKGETHYLQGLKWDFQLVQ
                     SEEVNAFCLPNGKIVFYEGIMKFTNTPDYIAVVMGHEIAHAVAQHGNERMSQQNLMSM
                     AGSLLSSAAGRKSDTTQALFEIAFGLGGQLGVILPYSRKHEYEADRIGLIIMAMAGYD
                     VEKAPIFWQAMSAKSGPSIPEFLSTHPSDENRIKKIIEAIPDAKTYVNK"
     gene            1280..>2211
                     /locus_tag="PLI69_06530"
     CDS             1280..>2211
                     /locus_tag="PLI69_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG42175.1"
                     /translation="MRSFASDNNSGVHPLVMEALIKANKDHAVGYGDDPWSAAAEKLL
                     KEHFGESATPFLVFNGTGANSVALQAVTRPFNSIICAATAHIFVDECGAPAKMSGCPL
                     IPVPTTDGKLTPELINPHLTNFGVCHHSQPGVVYISQATELGTLYTVEEIRRLADFIH
                     SYGLFLHMDGARLANACVALDSTFKEMTVDAGVDILSFGGTKNGMMMGEAVISFLPEI
                     SGNIKYYRKQSAQLASKQRYLAAQFLPYLGEGIWRDNAVHANNMALKLLQVLQKYPQI
                     RLSQKPQSNQLFFYAPKDFLEELIYKSVLLGFILL"
BASE COUNT          619 a          490 c          522 g          580 t
ORIGIN      
        1 agccaatgtc cagactttct acggcaccaa cataacccct caaagaggtg ctctaaacga
       61 gaatatctgg gctactcttg aaactactgt acgtgattgg tcaaaaaacc tggataccct
      121 atatgtggtc accggatgcg tgatcgaagg tagtaccgaa tttgtcaatg acaacgaagg
      181 aaagagggtt accgtaccta ccggctacta caaggccctt ttgggctata agcagaatgc
      241 atccattggt ggctctacca aaggttttgt aggcattgcc ttctattttg atcacaaagg
      301 ctattccaac gactatacta caatgatgaa gcagtcgatg acgatatccg ctcttgagaa
      361 aaaggttggt gtggatttct ttgtcaatct accggcaaaa ataggtgcag atcttgctca
      421 aaaggtagaa accggcattg atcctttctg gaaataaata aaaacaaaaa ataattttta
      481 tgaaacgcaa tttaaaatgg atagcctata cggtggttgc ggttatgata gcagttgcct
      541 gcagctcggt ggcttttacc ggacgtaaaa gagtgttgct ttactcagac tctcagattt
      601 cttcgctttc agagcaatcc tatcaggagt ttatgaccaa agcaaagctc tcaaccaaca
      661 agcagatgca agctaccgtg caggaagttg gtcgtaaaat gacaactgcg cttgagaagt
      721 atctcacttc aaaaggtgag acacactatc tccagggact taaatgggat tttcagttag
      781 tgcagtcgga agaggtcaat gctttctgcc tcccaaatgg caaaatagtc ttttatgagg
      841 gtataatgaa atttacaaat actcctgatt atattgccgt ggtaatggga cacgagatag
      901 ctcatgctgt tgcccagcat ggaaatgaac gtatgagcca gcagaatctg atgagcatgg
      961 ccggttctct cctaagttcg gcagctggcc gtaaatcaga taccactcag gcactttttg
     1021 agatcgcatt cggtctcgga gggcagcttg gagtgatctt gccctactcg cgtaaacacg
     1081 agtacgaggc tgacaggatt ggcctcatta taatggctat ggccggatat gatgttgaaa
     1141 aggcacctat tttctggcag gcaatgtcag cgaagagcgg accatccatc cctgaatttt
     1201 taagtacgca ccccagcgat gagaatcgta tcaaaaaaat cattgaagct atccctgacg
     1261 ctaaaactta tgtaaacaaa tgagaagttt cgcaagtgac aataattccg gagtccatcc
     1321 tttagtaatg gaggctttga taaaagccaa taaagaccat gcagttggct acggagatga
     1381 tccgtggagt gcagctgctg agaaattgtt aaaagaacat ttcggtgaga gtgctactcc
     1441 atttctcgta ttcaatggta ccggtgctaa ttcggtagcg cttcaggcag taacacgccc
     1501 cttcaactcc atcatctgtg cggctacggc gcatatcttc gtggatgagt gtggagcacc
     1561 ggctaagatg agcggctgtc ccctgattcc tgtcccgact accgacggta aacttacacc
     1621 cgaactgatt aacccccacc ttacgaattt cggcgtttgc caccactcac agccaggggt
     1681 agtctatatc tctcaggcta ctgaactggg taccctctat actgtggaag agataaggag
     1741 gctggcggac tttatccact cttatggact attcctccat atggatggtg cgcggttggc
     1801 taatgcctgt gtggcgctgg atagcacttt taaggagatg acggtagatg cgggagttga
     1861 tattctcagt tttggcggta caaagaatgg tatgatgatg ggagaggcgg taatcagttt
     1921 tcttccggaa atctccggga atattaagta ttaccgcaag cagtcggccc aactcgcctc
     1981 aaaacaacgc tatttggcag ctcagttctt gccctacctt ggtgaaggga tatggcgtga
     2041 caatgcagtt catgccaata acatggcatt aaagctgctc caagtgctgc agaaatatcc
     2101 tcaaataaga ctttcacaga aacctcagag caaccaactc ttcttctatg cgcctaagga
     2161 ttttctggag gagctgatct acaagagcgt actccttggc tttattctgc t
//
LOCUS       DAOSIE010000245         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_42733_length_2205_cov_2.945116, whole genome shotgun sequence.
ACCESSION   DAOSIE010000245 DAOSIE010000000
VERSION     DAOSIE010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42733_length_2205_cov_2.945116"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLI69_06535"
     CDS             complement(<1..848)
                     /locus_tag="PLI69_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42176.1"
                     /translation="MKKITTILFSILLTAGVSAQTAGEALLFSQHHYEGTARTLAMGN
                     AFTALGGDLGAIVINPASSAIYRYSQITLSPSIISSSGQAYYLGDYSGDSFTRMAMSN
                     LGFVMAFDTGNYSGLLNYNFGITLNRTNSFNSIMAAHGRTDKSSLLGSIAAGLSGVHV
                     SALESTASYDAFYNNNLSWPEVLAWETYLLANTPDSDYDYIGSTENIYDDSIIEVGGH
                     LDQSYFRKIRGGNSAFSFNFGGNISDFFFFGANLNLQSLNYELNETYGESAANPRDFQ
                     DGFESMT"
     gene            complement(875..1915)
                     /locus_tag="PLI69_06540"
     CDS             complement(875..1915)
                     /locus_tag="PLI69_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42177.1"
                     /translation="MKNKVFYLFLIAVTMVISSCGTTSYYSSASFDDGIYYRPTRESR
                     AAMVASNKELHESVKKPQSYILEDEEGNYYLVDEGTSYAERLRKFDSPTYTFYVDYGV
                     WASPWYNPWWGSYRYPYFGYPYNSWYYGWNSPWYTGWYDPWYTGWYDPWYYGYGGYGW
                     YYNYWNPWYYHGYYPGPGHIGNYYQDVAYGKRSAADGQSYRNTPRTTSGGSMYTRRSE
                     GRSAESYNSGGVYTGARSTTTSSGGSGTIYQRSSGSTTRSSGNVNSGTSRSSRRSDNY
                     NTDNSRSSSGSYSTGTSRSSSGSYSTGSSRSSSGSYSSGGGYSGSSGGGGYSGSSGSS
                     GSSGGGGTGGRR"
     gene            2003..>2205
                     /locus_tag="PLI69_06545"
     CDS             2003..>2205
                     /locus_tag="PLI69_06545"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662800.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOG42178.1"
                     /translation="MAKEVTSREENYSQWYNNLVLKADLAEQSAVRGCMVIKPYGYAI
                     WEKMQNQLDKMFKETGHVNAYFPL"
BASE COUNT          587 a          516 c          504 g          598 t
ORIGIN      
        1 gtcatagact caaatccatc ctgaaaatct ctcggatttg cagcactttc gccgtaggtt
       61 tcattcagtt catagttaag actctgaaga ttgagattgg ctccaaaaaa gaaaaaatcc
      121 gaaatattgc ctccgaagtt gaaggagaag gcgctgttac cgccgcgtat ttttcggaag
      181 taactctgat cgagatgacc tcctacttca attatgctgt catcgtaaat gttttcggta
      241 gaaccgatat aatcataatc ggaatcaggt gtattagcca acagatatgt ctcccatgcc
      301 agaacttccg gccaggagag attgttgttg tagaaagcat cataagaagc cgtgctctca
      361 agagccgata catgcactcc ggataggccg gcagcgatgg aacctaaaag tgatgacttg
      421 tcagtcctgc cgtgggctgc cattatggag ttgaaactat tggtgcggtt gagggtaatg
      481 ccgaaattat agttgagcag tcccgagtaa ttgccggtat caaatgccat gacaaacccc
      541 agattggaca ttgccatcct agtgaagctg tcacctgaat agtcccccaa atagtaggct
      601 tgaccactgc ttgatatgat ggaaggtgac agggtgattt gtgagtatct gtaaatggca
      661 ctggaggctg ggttgatgac gattgctccc aaatcacctc caagagcagt aaaggcattg
      721 cccattgcaa gcgtacgtgc cgtaccctca tagtgatgtt gggagaatag taaagcctct
      781 cctgcagttt gggcactcac tcctgccgtg agcaggattg aaaacaatat agttgtaatc
      841 tttttcattg ttttttcaca ttttgatttt tgaattatct ccttcctccg gtacctccgc
      901 caccactgct gccactgctg ccactgctgc cgctatatcc gccaccacca ctgcttccgc
      961 tatatccgcc accactgcta tagctgcctg aagagctcct gctgctacct gtactataac
     1021 tgccggaaga actcctgctc gtgccggtgc tataactacc ggaagagctt ctgctattgt
     1081 cggtattata attgtcggac ctgcgcgaag agcgtgatgt gccggaattg acgttgcccg
     1141 aactgcgggt agtgctgccg gaactgcgtt ggtagatggt accgctgcca ccgctcgacg
     1201 tagtggtgct gcgtgcaccc gtatatactc cgccggaatt gtagctttcg gcacttctgc
     1261 cttcgctcct tcttgtatac atggagccgc cggaagtagt gcgcggtgtg ttgcggtaac
     1321 tctggccgtc tgctgcagag cgtttaccgt aagcaacatc ctgatagtag ttgccgatgt
     1381 gacccggccc cggatagtat ccgtggtaat accatggatt ccaataattg tagtaccagc
     1441 catatccgcc atatccataa taccaggggt cgtaccagcc tgtataccat ggatcatacc
     1501 aaccggtata ccaggggcta ttccacccgt agtaccaaga gttataggga taaccgaaat
     1561 aggggtatct gtaggaaccc caccatggat tgtaccaagg ggaggcccaa acaccgtaat
     1621 caacatagaa ggtataggta gggctatcaa attttctcaa tctttctgca taagatgttc
     1681 cctcatctac gagatagtaa tttccctctt catcctccag aatatatgat tgcggtttct
     1741 ttacagattc gtgcagctct ttattagatg caaccattgc ggccctcgac tcgcgtgtgg
     1801 gcctgtagta tatgccgtca tcaaatgatg cggaagaata ataacttgtg gtaccacaag
     1861 agcttatcac cattgtaacg gctataagaa acagatagaa aactttgttt ttcattttta
     1921 cctcctgtgt tagataattt tgtacttttg cagtcgcatt tcattacaaa tatatcaatt
     1981 tttataccaa atacaacaaa aaatggcaaa agaagtaaca agtagggaag aaaattactc
     2041 ccaatggtac aacaatctcg tgttaaaggc ggatcttgct gaacagtcgg cagttagagg
     2101 atgtatggtg ataaaacctt acggatatgc catctgggaa aagatgcaaa accagcttga
     2161 caaaatgttc aaagagacag gtcatgtcaa tgcttatttc cctct
//
LOCUS       DAOSIE010000246         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_42798_length_2203_cov_2.799814, whole genome shotgun sequence.
ACCESSION   DAOSIE010000246 DAOSIE010000000
VERSION     DAOSIE010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42798_length_2203_cov_2.799814"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLI69_06550"
     CDS             complement(<1..307)
                     /locus_tag="PLI69_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOG42179.1"
                     /translation="MGANAQTDSVAQEKFDVKGLILHHLADSYEWSLISSEDWHVAIP
                     LPVILFSKTSGLHLFLSSKFEQGKQAYKGFQIAADGKYQGKIVELSGEGSEVRPLDLS
                     "
     gene            complement(379..795)
                     /locus_tag="PLI69_06555"
     CDS             complement(379..795)
                     /locus_tag="PLI69_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42180.1"
                     /translation="MKKFVRKFWIIHTLLLLALGVGVWVALIFLFPEVEVRGYFVIPL
                     YFYLMGVALFLRFRRISIDKPSKTTNMYMQMRVIKVLISLIIIVVYWLLNKSGVRPFA
                     IVFAVFYLIYLIWETFLFLRMEKHIRYIKSQNEPLN"
     gene            complement(801..1049)
                     /locus_tag="PLI69_06560"
     CDS             complement(801..1049)
                     /locus_tag="PLI69_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOG42181.1"
                     /translation="MKLLIRTLESTVFSGEVTSVTLPGEIGSFQVLKNHAPLVSSLTK
                     GTILFVANGSVREIAIEGGFVEVKDNTISLYTDIVEKE"
     gene            complement(1053..>2203)
                     /gene="atpD"
                     /locus_tag="PLI69_06565"
     CDS             complement(1053..>2203)
                     /gene="atpD"
                     /locus_tag="PLI69_06565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOG42182.1"
                     /translation="APKFDYLVTSEEMLTTGIKVIDLLQPYLKGGKIGLFGGAGVGKT
                     VIIMELINNIAKGHKGYSVFAGVGERTREGNDLLREMIESGVIRYGEEFNRSMAEGHW
                     DLSVVDYNELEKSQTTLVFGQMNEPPGARASVAHTGLAVAEAFRDSGGEGSDILLFID
                     NIFRFVQAGSEVSALLGRMPSAVGYQPTLASEMGNLQERITSTKYGSITSVQAVYVPA
                     DDLTDPAPATTFSYLDATTVLSRKIASLGIYPAVDPLESSSRILNPNVVGEDHYDTAM
                     RVKQILQRYKELQDIISILGMDELSDEDRLTVNRARKVQRFLSQPFHMAEQYTGQPGV
                     MVSVKDTIKGFKMILDGELDGYPEASFMNVGTIEEAIAKGDKLLKQVK"
BASE COUNT          616 a          578 c          427 g          582 t
ORIGIN      
        1 gcgacaaatc cagaggacgg acttcgcttc cttcaccgct caattccacg attttccctt
       61 gatatttccc atcggcagcg atctgaaagc ctttataggc ctgtttccct tgttcaaatt
      121 tggatgaaag aaagaggtgc aaccctgacg ttttactaaa caggattacc ggtagaggaa
      181 tagcaacatg ccaatcttcc gaactaatca aactccattc gtaggaatcc gccaagtggt
      241 gcaaaatcaa ccccttaaca tcgaactttt cctgagccac gctgtctgtc tgggcattcg
      301 ctcccatcgt caagagaaga aggatgcaaa gaataattgg cttatatgtt acctgaagtt
      361 tattcatctg ccttaaaatc aattaagcgg ctcattttga cttttgatat acctgatatg
      421 cttttccatc cgaaggaaaa gaaatgtttc ccaaatcagg taaatcagat aaaacaccgc
      481 aaaaacaatc gcgaaaggac ggacccccga cttgttgagt agccagtaaa ccactatgat
      541 aataagggag atcaacactt ttatcaccct catctgcata tacatattcg ttgtcttgga
      601 gggcttgtca atcgaaatcc tgcgaaatct caaaaataga gcaactccca tcagataaaa
      661 gtagagcgga atgacaaagt accctcttac ctctacctca ggaaaaagaa atattaaggc
      721 aacccatact cctactccca aagcaagtag aagcagcgta tgaataatcc aaaattttct
      781 tacaaacttt ttcataagaa tcactccttt tcaacaatat ccgtatagag acttattgtg
      841 ttatctttta cctctacaaa accgccctct atcgcaattt ccctgacact cccattagcc
      901 acgaacaaaa tcgtaccttt agttaacgaa gagacgagcg gggcgtgatt tttcaacacc
      961 tggaaagacc ctatctcccc gggcaaggtt actgaagtaa cttcaccgga aaaaacagtc
     1021 gattcaagag tgcggatgag tagtttcatc acttatttta cttgttttaa caacttgtca
     1081 cctttagcaa ttgcctcttc aatggttccc acattcatga acgatgcttc cggataacca
     1141 tccagctcac catccagtat catcttaaat ccttttatag tatccttcac ggagaccata
     1201 actcccggct gcccggtata ttgttccgcc atgtggaagg gctgagaaag gaaacgctga
     1261 accttacgtg ctctgtttac agtaagcctg tcctcgtcgg acagctcatc cattcccaat
     1321 atggaaatga tatcttgtag ctctttgtag cgctggagaa tctgttttac ccgcatagcg
     1381 gtatcatagt gatcctcgcc cacaacattg gggtttagga tgcgggaaga ggactccaaa
     1441 gggtccaccg cgggataaat acccaatgat gcgatctttc tgctcaaaac agtagttgca
     1501 tccagataac tgaaagtggt agcaggcgca gggtcggtca agtcgtcagc cggcacataa
     1561 accgcctgta cggaagtgat tgaaccatac ttggtagagg taattcgctc ttgcagattg
     1621 cccatctcag aggccaatgt cggctgatag cccaccgccg aaggcatacg ccccaacaga
     1681 gctgaaacct cggaacccgc ctgcacaaac ctgaatatgt tgtctatgaa gagcaaaata
     1741 tcgcttcctt caccgccact atccctaaaa gcttctgcta cagcaaggcc ggtgtgtgct
     1801 accgaagcac gtgctccggg cggttcgttc atctgtccga atacaagggt agtttgagat
     1861 ttttccaact cgttatagtc caccacactc aaatcccaat gcccctccgc catgctcctg
     1921 ttgaactcct cgccatatct tatcaccccc gactctatca tctcacgcag caaatcgttt
     1981 ccttcacgcg tacgctcccc tactccggca aagaccgaat aacctttgtg tccttttgct
     2041 atgttgttga taagctccat gataatcacg gttttgccca ctcctgcacc tccgaaaagt
     2101 ccgatttttc cacctttcag gtagggttgc agcaggtcga ttaccttgat tccggtagtg
     2161 agcatctcct ccgatgtaac cagatagtca aatttgggtg ctt
//
LOCUS       DAOSIE010000247         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_43296_length_2184_cov_3.187882, whole genome shotgun sequence.
ACCESSION   DAOSIE010000247 DAOSIE010000000
VERSION     DAOSIE010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43296_length_2184_cov_3.187882"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLI69_06570"
     CDS             complement(<1..186)
                     /locus_tag="PLI69_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOG42183.1"
                     /translation="MTKLYLDHIAEVMNAKVWQIENCISLFEEGATIPFISRYRKERT
                     GALDETQVSEVRYWYLRF"
     gene            complement(179..1942)
                     /locus_tag="PLI69_06575"
     CDS             complement(179..1942)
                     /locus_tag="PLI69_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOG42184.1"
                     /translation="MKLFSGIKKGFSSISERTQLVLLSFVVGLGCGFAAVLLKTLIKW
                     IHHGLTFWFDKPSDGYLYLIYPGVGMLLSFLFVRYFVKSDISHGVTKVLLSISRNESR
                     IKPHNTWTSIVASSVTIGFGGSVGAEAPIVYTGAAIGSNVGRSMGMSYKNMTILIGCG
                     AAGAVAGIFKAPLAGVLFTLEILLFNISMSSILPLLISSVTATVVSYLFLGDAVAISN
                     TIETFSMGNLPYYFVLGIFCGIISLYFIRTTLRVEDKMRRVRKPINRWIIGATAMGVL
                     VFLFPPLYGEGYNSLTLMLNNNPIEMAGGIIGERVLANKWSVLLFFTAILLVKVYATA
                     FTNAGGGVGGTFGPTLFTGALAGFVVARFINITGIHVIPEANFVLVGMGGLMAGVMQA
                     PMTAIFLIAEITGGYDLLIPLILTSAISFATIRLVEQYSIYTKRIAQQGDLLTHDSDK
                     AVLILLKTSDLIETDFSTISIDGNLGDLVKVIATSCRNIFPVVDDSNQFQGIVFLDDV
                     RTTMFDRSLYESIKIKDIMKNAPAHVCDDEKMESVMNKFEVTQAWNLPVVDKNNKYLG
                     FVSKSKIFSSYREQLQQVSHD"
BASE COUNT          666 a          526 c          441 g          551 t
ORIGIN      
        1 gaaacggaga taccagtatc gtacttcgga aacctgagtt tcatccagag cgcccgtgcg
       61 ttctttacgg tatcggctta taaacggaat tgttgcaccc tcctcaaaga gcgagatgca
      121 gttctcgatc tgccatacct tggcattcat tacctctgca atatggtcga gatagagttt
      181 agtcatggga aacctgttgt aattgttctc tgtaggaaga gaatattttg gacttggaaa
      241 caaatcccaa gtacttattg tttttgtcaa ccaccgggag attccaggct tgggtaactt
      301 caaatttatt cattacactc tccatcttct catcgtcaca gacatgggcg ggggcatttt
      361 tcataatatc ctttatctta atgctctcat atagactgcg gtcgaacata gtagtcctca
      421 cgtcatccag aaagactatc ccctgaaact ggttggaatc atccacgaca gggaaaatat
      481 tacggcagga agtggcaatc actttgacca gatctcccag gttgccatca atgctgattg
      541 tcgaaaaatc cgtctcgata agatccgagg ttttaagcaa tattagaacc gccttatcac
      601 tatcatgggt aagcaaatcg ccctgttggg caatacgttt tgtatagatg gaatattgct
      661 ctaccaaacg aattgtggca aacgagatgg cggaggtaag gataagaggt atcagaagat
      721 catatccacc ggtaatctct gcaatgagaa atatggcagt cataggtgcc tgcatgacac
      781 cagccatcag tccgcccatc cctaccagta caaaattagc ctctggaata acatgtattc
      841 cggtaatatt gatgaaacgc gcaacaacaa agcctgcaag agctccggta aagagagttg
      901 gaccgaaagt gccgccaact ccgcctcccg catttgtaaa agctgtagcg tacactttaa
      961 ccagcagaat tgccgtaaaa aagagaagta cagaccattt attggccaaa acacgctcgc
     1021 caattatccc tcccgccatc tcgataggat tattgttgag cataagcgta agggaattgt
     1081 aaccttctcc ataaagcggc gggaaaagga agactaaaac acccattgct gttgctccga
     1141 taatccagcg atttataggc ttacgtaccc gtctcatttt gtcctcaacc ctcagagtgg
     1201 tcctgatgaa gtagagtgag attataccgc aaaaaattcc caggacgaaa taatagggta
     1261 aattacccat tgaaaaggtc tcgatggtat tggaaatagc tactgcgtca cccagaaaaa
     1321 gatatgaaac caccgtggcc gtaactgaag aaatcagcag cggtaagatg gaagacatgg
     1381 agatattgaa aagcaatatc tccagagtaa agagcacacc ggccagcgga gccttaaaaa
     1441 tacccgctac agcaccagca gccccacatc ctatcagaat agtcatattc ttgtatgaca
     1501 tccccatact ccttccgaca ttggagccta tcgccgcacc tgtataaact ataggtgcct
     1561 cggcacctac cgaaccacca aaaccgattg ttacggaact ggccactatc gaagtccagg
     1621 tgttgtgagg ttttattcgc gattcgtttc gtgaaatgga gagcaaaacc ttggtaactc
     1681 catggcttat atcactcttg acaaaatatc tgacaaacag gaatgaaagc agcattccta
     1741 cacccggata gatgagatat aaatatccgt ctgagggttt atcgaaccag aatgtcaggc
     1801 catgatgaat ccattttatc agggtcttga gcaaaactgc cgcaaaacca caaccaagac
     1861 ccacaacaaa ggagagcaac accaattgtg tacgctcgga gatggaggaa aagccttttt
     1921 ttattccgga aaataatttc actcgttttt taatcaaaaa gagcaacctt tctggccact
     1981 ctctgtatgt tggccgttag ctgttggccg ttggctattg cttattcaaa aaatcttaat
     2041 tgataactcc catattgcca accacacata aatcccgact cccctacacc ctacacccta
     2101 caccctacag cctacaccca aaaccaactc tctgtctcgt cctgaaaaaa gttgacacat
     2161 tttgttaaac tttactgttg tcat
//
LOCUS       DAOSIE010000248         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_43425_length_2179_cov_4.293315, whole genome shotgun sequence.
ACCESSION   DAOSIE010000248 DAOSIE010000000
VERSION     DAOSIE010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43425_length_2179_cov_4.293315"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1637)
                     /gene="rlmD"
                     /locus_tag="PLI69_06580"
     CDS             complement(180..1637)
                     /gene="rlmD"
                     /locus_tag="PLI69_06580"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOG42185.1"
                     /translation="MARKQKPLLQSIVIESIAAEGKALAHIDGKVLFVPQAIPGDVVD
                     VQVTRSRRGYMEGYITRQVTLSPHRIEPFCCHFGRCGGCKWQALPYPMQLKYKQQQVE
                     DQLVRIGRLDLPGILPILGSDKTVEYRNKLEYTFSNRRWILKEEESESLSNKERYGVG
                     FHISGMFDKVLDITKCHLQREPTNALRLFVRDYALYHNLSFFDLREQHGFMRTLIVRT
                     SSTGELMVIVVLGKDAGDSAGPEARDKRNALLDALSAAFPEINSLYYVINPKGNDTIN
                     DLECVLYKGEDAIYEQMESLRFKIGPKSFYQTNSEQAYRLYSVVREYADETISRMSDG
                     KKPVIYDLYTGTGTIALFLSSLASKVVGIDYVPEAIDDARLNAGINSIDNAAFFAGDM
                     KDILNADFILNNGAPDIVVLDPPRAGIHPDVAQVLLEASPEEIIYVSCNPATQARDLA
                     ILSKSYRIVAVQPVDMFPHTHHVENVVNLTKIKDE"
     gene            1738..>2179
                     /locus_tag="PLI69_06585"
     CDS             1738..>2179
                     /locus_tag="PLI69_06585"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG42186.1"
                     /translation="MKRYLSIFMMSLLSMTTLFAYAENEKEQNDFFVIYGKIIDVDTR
                     TPLYFASISLDGTNIANVTNSEGFFSLKLPLDISGERIITTSYLGYLSTTLRVSDFRG
                     SSDSKPLVISMKAVPLSLDPATIRAYDARSLFYEAFSKVKQNYPQ"
BASE COUNT          596 a          486 c          458 g          639 t
ORIGIN      
        1 acccggatta tccgcagggg cacctgagag atatcccatg tgcagcatca tgcctccctg
       61 gaaaccgtta taacgaaaaa ccccacctct ttgttcctct tgggctgaca gggattcggc
      121 cgaagtaata acgatcgaca gagctacgaa aattttaata atataatctc ttttcattct
      181 cattcatctt taattttggt aagattcacc acattttcca catgatgtgt gtggggaaac
      241 atatctacag gctgaactgc gacaatacgg tagctttttg aaaggatggc aaggtcgcgt
      301 gcctgggttg ccggattaca gctgacatag ataatctctt ccggtgaggc ttcaaggagc
      361 acttgagcca catcgggatg gatccccgcc ctgggagggt cgagtaccac aatatcaggc
      421 gcaccgttat tcagtataaa gtcggcattt aatatatctt tcatatcacc cgcaaaaaaa
      481 gcggcattat ctattgagtt aatccctgca ttgagtctgg catcatctat cgcctccggt
      541 acatagtcga tacccacaac ttttgatgca agagatgaga gaaaaagtgc aatggtgcct
      601 gttccggtgt agagatcgta tattacaggt ttcttcccat cgctcattcg tgatattgtc
      661 tcatctgcat attcccttac tacagaatag agacgatatg cttgctccga attggtttgg
      721 tagaaagatt tagggccaat tttgaaacga agagactcca tttgctcata aatagcatcc
      781 tctcctttat aaagcacgca ctcaaggtca ttgatagtgt cgtttccctt gggattaatc
      841 acgtaataga gagagtttat ctcaggaaaa gcagccgaga gtgcatccaa aagggcatta
      901 cgcttatctc tcgcttcggg acctgcacta tctccggcat ctttacctaa tacaacgatt
      961 accatcagtt ctcctgtaga tgaagtacga acaatcaacg tacgcataaa tccgtgctgt
     1021 tcgcgcagat cgaaaaaact cagattgtgg tacaacgcgt aatcgcgtac aaagaggcgc
     1081 agtgcgttgg ttggttcacg ctgtaggtgg catttggtga tatccaacac tttgtcgaac
     1141 atcccgctga tatgaaatcc tacaccgtaa cgctctttat tggagagtga ttcggactct
     1201 tcttctttta gtatccatct gcgattggag aaggtgtatt ctagcttgtt gcggtattcc
     1261 acagttttat cggagccaag gatgggcaaa attcccggaa gatcaagtcg accgatccta
     1321 acaagttggt cctcgacctg ttgttgcttg tacttcagct gcatagggta ggggagtgcc
     1381 tgccatttgc aacctccgca cctgccaaaa tggcagcaga aaggttcaat gcgatggggt
     1441 gagagggtta cctgacgggt aatatatccc tccatatagc ctcgtctgct tctggtaacc
     1501 tgcacatcta ccacatctcc gggtattgcc tgaggcacaa aaagtacttt gccatcaata
     1561 tgagccaaag ctttgccttc ggcagctatt gattcgatca caatactctg taacagtggt
     1621 ttttgctttc ttgccatttg gtaaactttt tgcgaatggc aaagatatta agaatctttt
     1681 gtacaataaa acagatttat tatttttata gttagaaacg aaccaattca cacagttatg
     1741 aaaaggtatt tatccatatt tatgatgtca ttgctttcaa tgacaaccct cttcgcttat
     1801 gcagaaaatg agaaggagca aaatgatttt tttgtcatat acggtaaaat aatcgatgta
     1861 gatacaagaa ctccgctcta tttcgcttcc attagtcttg acggtaccaa tatcgccaat
     1921 gttaccaatt ctgagggctt tttctctttg aaacttcctt tggatatctc aggtgaacgt
     1981 attatcacca caagctacct tggatatttg tcaactacac taagagtgtc ggattttaga
     2041 ggctcaagtg actctaaacc actggtaatc agcatgaagg ctgtaccttt gtcacttgat
     2101 ccggctacaa ttcgcgctta cgacgcgcgt tctctttttt atgaggcatt tagcaaagtc
     2161 aaacaaaatt atcctcaga
//
LOCUS       DAOSIE010000249         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_44044_length_2157_cov_2.595623, whole genome shotgun sequence.
ACCESSION   DAOSIE010000249 DAOSIE010000000
VERSION     DAOSIE010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44044_length_2157_cov_2.595623"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1747)
                     /locus_tag="PLI69_06590"
     CDS             complement(47..1747)
                     /locus_tag="PLI69_06590"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOG42187.1"
                     /translation="MKRLLLTFLTVIAAFTATAVNPAIRDIEITYILTPDGHAKVTEV
                     WDVSVASGTEWYLVKYNMGSQVTITEFSVTDETGVDFINEGDWDLDRSLNQKAGKCGI
                     YRKGGDSYELCWGVGSYGDHTYTVKYTINNFILSMNDYDAIHFQFISPDLSSAPEHAK
                     VTIRYQDDLYQLDTANSLVWGFGYEGRCEFIDGAIVAESDAPFKKKSSVIVLARFDKG
                     MFHDLINIDDRLFEERHALAMKGSHFDGEPSESSYEDFEAKDALIPILWTLGIILAGV
                     GVGKQRIKYNNFSNFGVKKLKDIGWSREIPYNGNLLATNYVITKAPSLFKTRNSYAIA
                     SAYILRMIQKNILITGKDEKGKITISFNDKADLDKLESPEKSLFNMMKQASGANLILE
                     DKEFSRWAKKNKTTLYDWTNSISNKGVKTLESNGFYSGYKFTPSGVEANNKVLGFFKY
                     LQDFTLINERKSAEVHLWQDYLVFAALFGIADKVAKELKDIDPIAFESVVNYDFDTFS
                     TILHQTRMLSSAITLNSYVSTSSGGSFSGTSSWSGGGGGSSFGGGGGFSGGGSGGGSR
                     "
     gene            complement(1747..>2157)
                     /locus_tag="PLI69_06595"
     CDS             complement(1747..>2157)
                     /locus_tag="PLI69_06595"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOG42188.1"
                     /translation="DALTAQADLIKSYDEQEYKSLKDVIAQRTSITGSSSAADANIQE
                     TILTDAMRRIAVVVEQYPNLKANEMYVKTMDSVNLYENQVRMSRMVYNDSVTNYNRLI
                     RQIPSNIVASLFGFRSKDYLEENQAKAEMPSMRK"
BASE COUNT          565 a          496 c          455 g          641 t
ORIGIN      
        1 tttagtcgca aaggtaattt ttttttgata gtccggcaaa tggaatttat ctgctccctc
       61 ctccggaacc tcctccggaa aaaccacctc caccaccgaa tgaagagcca cctccaccac
      121 cactccagga gctggtgcct gagaatgaac cacctgacga ggttgataca taagagttca
      181 gcgtgattgc actggaaagc attctcgtct gatggaggat agtggagaat gtatcaaagt
      241 cgtagttgac cacactctca aacgctatgg gatcaatatc cttaagctct ttcgccacct
      301 tgtcggcaat gccgaaaagg gctgcaaata ccagataatc ttgccataag tgtacctcgg
      361 ctgattttct ttcattaatc agcgtaaaat cctgcaggta cttgaaaaac cccagtactt
      421 tattgttggc ctcgacacct gaaggggtaa atttgtatcc tgagtaaaat ccattgctct
      481 ccagtgtttt aactccctta ttgctgatgg aatttgtcca gtcatagagc gtggttttat
      541 tctttttggc ccatctggaa aactctttgt cttcgaggat caggtttgct ccgctggcct
      601 gcttcatcat gttgaaaagt gacttttcgg gagattcaag cttatcgaga tctgccttgt
      661 cattgaaaga gatagttatt tttcctttct cgtctttgcc tgtaatcaga atatttttct
      721 gtatcatcct aaggatatag gctgacgcta tagcataact attgcgtgtc ttaaagaggg
      781 aaggagcttt cgtaatgaca taattggttg caagcaagtt accattgtat ggaatttccc
      841 gtgaccaccc gatatctttg agttttttca ccccaaaatt tgagaaatta ttatatttta
      901 ttctctgttt acctacaccc acaccggcca ggataatgcc caaagtccat aggatcggaa
      961 taagggcatc tttagcctca aaatcttcgt atgaagattc cgatggttct ccatcaaagt
     1021 gactgccctt cattgccaga gcatggcgct cttcaaatag acggtcatca atgttgataa
     1081 gatcgtgaaa catccctttg tcaaaacggg ccaacactat gactgaactt tttttcttga
     1141 aaggggcatc cgattcggcc acaatggcgc catcaatgaa ttcacatcta ccttcgtacc
     1201 cgaaacccca tacaagagag tttgctgtgt ccagctggta taggtcatcc tgatacctga
     1261 tagttacttt cgcatgttcg ggggcggaag agaggtcagg ggagatgaac tggaaatgta
     1321 tggcatcata atcattcatc gacaagatga aattattgat ggtgtatttt actgtatagg
     1381 tgtgatcccc ataggagcca acaccccagc acaattcgta gctatcacct cccttacggt
     1441 aaataccaca cttcccggcc ttctgattga ggctacgatc taagtcccaa tctccttcat
     1501 ttatgaagtc aacaccagtc tcatcggtta ccgaaaactc cgtaatggtt acctgagaac
     1561 ccatattgta tttgaccagg taccattcgg tgccgctggc aactgagaca tcccacactt
     1621 ccgtcacttt tgcgtgacca tcaggtgtaa gtatgtaagt tatttctata tcccgaatgg
     1681 cgggatttac cgcagtcgca gtaaaagctg caattacggt caaaaatgta agcagcagac
     1741 gtttcattac tttctcattg agggcatttc agccttggcc tgattctcct caagataatc
     1801 cttggatctg aaaccgaata aagaggctac aatattggaa ggaatctgac ggatgagacg
     1861 gttatagttg gttacgctgt cattgtaaac catacgactc atgcgtacct gattctcata
     1921 taaattgacg ctatccatcg tcttgacata catctcgttg gctttgaggt tgggatattg
     1981 ctcaactaca acggctatac gtctcatggc atctgtaagg attgtttcct gtatgttggc
     2041 atcagctgca gaactgctgc cggtgatgct tgtgcgctgt gcaatgacat ctttgagaga
     2101 cttgtactct tgttcgtcat atgatttgat gagatccgcc tgtgccgtaa gggcatc
//
LOCUS       DAOSIE010000250         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_44505_length_2140_cov_2.788969, whole genome shotgun sequence.
ACCESSION   DAOSIE010000250 DAOSIE010000000
VERSION     DAOSIE010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44505_length_2140_cov_2.788969"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLI69_06600"
     CDS             complement(<1..501)
                     /locus_tag="PLI69_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42189.1"
                     /translation="MEKKISTKEILAGMLGCQELIDDLGLDLKKKSLLECFEEVIERA
                     RKNREFGREYDEYFCLNDKLIAAKRFELETIAEEYGISEWQAALFAIVIELCKGDDVN
                     QRRLAEQLKTSFVSFLSLEKDLLELEKLRLIKRNKWGSIRVSREVLEMLRENKPFRKG
                     SSTGLTT"
     gene            complement(746..2026)
                     /gene="glyA"
                     /locus_tag="PLI69_06605"
     CDS             complement(746..2026)
                     /gene="glyA"
                     /locus_tag="PLI69_06605"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOG42190.1"
                     /translation="MKQDVQISELLRKEEARQTKGIELIASENFVSDQVLAALGSVCT
                     NKYAEGYPGARYYGGCEVVDQIEQLAIDRLCQLFDAEYANVQPHSGAQANMAVFMVCL
                     KPGDTFMGLDLDHGGHLTHGSPVNMSGILYNAIGYQLNRETGMVDYDQMERKALKYRP
                     KLLVGGASAYSREWDWERMRAIADKIGAIFMVDMAHPAGLIAAGLMNNPVKHAHIVTS
                     TTHKTLRGPRGGIILIGKDFDNPWGIKTRKGVVRKMSSLINSSVFPGIQGGPLEHCIA
                     AKAVSFYEALQPEFKTYQEQVIKNASAMAKAFVEKGYHVVSGGTDNHLMLIDFRSKFP
                     DLTGRVAEDVLGKADITVNKNMVPFDSRSAFQTSGMRVGTPAVTSRGLVEKDMAGIVD
                     MIDKVLSNLDDDNVIAQVKEQVRMKMSGLPLNRW"
BASE COUNT          549 a          546 c          412 g          633 t
ORIGIN      
        1 tgttgtcaat cctgttgagg agccttttcg gaaaggctta ttttcgcgca acatctctaa
       61 aacctctctc gaaactctaa tcgaacccca cttatttctt ttaattaaac gcagcttctc
      121 aagctcaagc agatccttct ctaatgagag aaatgagacg aaacttgttt tgagttgttc
      181 ggcaagtctc ctctgattca catcatctcc cttacatagt tcaatcacaa tagcaaataa
      241 cgccgcctgc cactcgctga ttccatattc ttctgcaatc gtctccaact cgaagcgttt
      301 agcagcaatt aatttatcgt taagacagaa gtactcatca tactcccgac caaactccct
      361 attctttcgc gccctctcaa ttacctcctc aaaacactct aacagacttt tctttttcaa
      421 atcaagtccc aaatcatcaa tcagctcttg acaccccagc atacctgcca ggatctcttt
      481 tgtagatatt ttcttttcca taactgtgct cctttttact tttgtcactg caagaaattt
      541 tttattactg caaagaaaaa catttatact gccaaaaagt ggcagtacaa gttattttgc
      601 ccaaattctc acaatttaag caataccccg caccccgtaa ctcgaacctt gtaccactca
      661 aaaccccacg taacccgcaa ctcgcaacac aaaaaaacct gaaagtcata aactttcagg
      721 tttttttgtc aagcccgaag ggcttctacc acctatttag cggcagaccg ctcatcttca
      781 ttcttacttg ttcttttacc tgtgcgatta cattgtcgtc atcaagattg ctgagtacct
      841 tgtcaatcat atcaacgatg ccggccatat ccttttcaac cagaccgcgt gaggttactg
      901 cgggagtacc taccctcata ccggaagttt ggaatgctga gcggctgtca aaaggcacca
      961 tgtttttatt aacggtaata tctgcttttc cgagtacatc ctcagctacc ctaccggtca
     1021 ggtcggggaa cttggagcgg aagtcgataa gcatgaggtg attgtccgta cctcctgata
     1081 ctacatgata acccttctct acaaatgcct tggccattgc agaagcattc tttattactt
     1141 gttcctgata tgttttgaac tccggctgga gggcttcata gaaggaaacg gctttagctg
     1201 cgatgcagtg ctcgagggga ccgccctgaa taccggggaa tacgcttgaa ttgatcagtg
     1261 aagacatttt cctgaccaca ccttttcttg ttttaattcc ccacgggttg tcgaagtctt
     1321 ttccgataag gattatgcct ccacggggac ctctgagagt cttgtgagtg gtagaggtaa
     1381 cgatatgggc atgttttaca gggttattca tcagacctgc tgcaataagt cccgcagggt
     1441 gagccatatc caccataaag attgcaccta tcttatcggc aatggcacgc atcctttccc
     1501 agtcccactc tctcgagtaa gcggatgcac cgccgactag gagttttgga cggtacttca
     1561 aggccttgcg ctccatctgg tcataatcta ccataccggt ctcacggttg agctggtaac
     1621 cgatggcatt gtagaggatg cctgacatat ttacgggaga accgtgcgtg agatggccac
     1681 catgatccag atccagaccc ataaaggtgt cacccggttt tagacaaacc ataaatacag
     1741 ccatattagc ctgtgctccc gagtggggct gtacattggc atattcagca tcgaagagct
     1801 ggcatagacg atcgattgca agttgttcga tctggtctac tacctcacag ccgccatagt
     1861 atcttgcgcc gggatagcct tcggcgtatt tgttggtgca aactgaaccc aatgctgcaa
     1921 gcacctggtc gcttacaaag ttctctgatg ctatcagctc aataccttta gtctggcggg
     1981 cctcttcttt tctaagtaat tcggatattt gaacgtcttg tttcattgtt atattgtttg
     2041 tataaatatt ttcgtacttt tgtaacccgg cacaggcagg attgaactac aaagataact
     2101 tttttacgac tttttccaac tatgttccga aaacttttga
//
LOCUS       DAOSIE010000251         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_44721_length_2132_cov_4.771305, whole genome shotgun sequence.
ACCESSION   DAOSIE010000251 DAOSIE010000000
VERSION     DAOSIE010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44721_length_2132_cov_4.771305"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PLI69_06610"
     CDS             complement(<1..1085)
                     /locus_tag="PLI69_06610"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOG42191.1"
                     /translation="MKKPELKILDRYIIRKFIGTYFFILMIAVLIIIIFDISEKIDKF
                     VDNQVPLKEIVFEYFGGFIPWMLNSFSPLFVFLSVIFFTSKLAQRSEIIAMLSGGMSF
                     NRIMRPYMLSAAFIATFSLVLGLFIIPSANQMRLEFENKYIKQQQTASATSKIHYQIA
                     PGEFVFVEQFSSWANTAYRFTLETIEDNRIVSKLSAESAVWDTTFNGWKLRNWYIRDF
                     HNEESEIVRTGKELDTVINLTIEDFYRRRNIAESLSHKELNELIDVQTMRGDALVLHA
                     LIEKNNRIAMPFSAFVLTVMGVSLSSRKRRGGIGLNIGIGIALSFAYILFMRFSEMPV
                     HTGALSPAFALWLPNIIFSIIALILYR"
     gene            complement(1099..>2132)
                     /gene="tgt"
                     /locus_tag="PLI69_06615"
     CDS             complement(1099..>2132)
                     /gene="tgt"
                     /locus_tag="PLI69_06615"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOG42192.1"
                     /translation="VGTVGSVKGVYHRDLEEDIGAQIILGNTYHLYLRPGPDILRQAG
                     GLHNFISWEKPILTDSGGFQVFSLAQCRKLTDEGYEFQSHIDGSKHLFTPENNVDIQR
                     IIGADIIMALDECCPGDADCRYAAESLDLTMKWLKRCIARFDSTEPLYGHHQAFFPIV
                     QGCIYPDLRRKAAEELTALDREGYAIGGLSVGEPTELMYQMIEEVHPILPQDKPRYLM
                     GVGTPANILESIARGIDMFDCVMPTRNGRNGMLFTGEGIINIRNRKWAEDFSPIDPDG
                     TSFVDRRYTKAYLRHLFIAGEMLGAQIASEHNLAFYLNLVKMAREHILKGDFNSWKCA
                     MVKKLQTRL"
BASE COUNT          526 a          487 c          553 g          566 t
ORIGIN      
        1 atcctgtaga ggatcagtgc tattattgag aatattatat tcggcagcca tagtgcaaac
       61 gccggagaaa gtgcgccggt gtggacaggc atttcgctga agcgcatgaa gagaatgtat
      121 gcaaaactca gtgctatgcc tataccgatg ttgaggccga ttcctccgcg ccgtttgcgg
      181 gaggagaggg acacgcccat tacagtcagc acaaatgctg aaaatggcat cgcaatacgg
      241 ttatttttct ctataagagc atgcagtaca agtgcatctc cgcgcatggt ctgtacgtct
      301 atcagttcgt tgagttcttt gtgggagagg gactctgcga tgtttctgcg ccggtagaag
      361 tcctcgatag tgaggttgat aacagtatca agttccttgc cggtacgcac tatttccgat
      421 tcttcattgt gaaaatccct tatataccag ttgcgaagtt tccatccgtt gaaagtggtg
      481 tcccatactg ccgactcggc tgaaagtttg gatacaatgc ggttgtcctc aatggtctcg
      541 agggtaaatc tgtacgcggt gttggcccag gagctgaact gctctacaaa gacaaactcg
      601 cccggagcta tctggtagtg gattttactg gttgcagagg ccgtttgctg ttgttttata
      661 tatttatttt caaactcgag acgcatctga tttgccgaag gtattatgaa gagacccagc
      721 acaaggctga aggttgcgat gaaagcggcg gagagcatgt agggcctcat tattcggttg
      781 aaactcatcc cgcctgagag catggctata atctcgcttc tctgcgccag tttggatgtg
      841 aaaaaaatta ctgagaggaa gacgaagaga gggctgaaac tattgagcat ccagggtatg
      901 aatccaccaa aatattcaaa gacaatctcc ttgaggggta cctgattatc aacgaatttg
      961 tcgattttct cactgatgtc aaatattatg atgataagca ccgcaatcat caggatgaag
     1021 aaataggtac ctatgaactt cctgataata tatctgtcca atatttttag ttcgggcttc
     1081 ttcatcttgc cgtagttctt acagtcttgt ttgaagtttc tttaccatgg cgcatttcca
     1141 ggagttgaaa tcacccttga gtatgtgctc gcgggccatt ttcactagat tgaggtaaaa
     1201 agcgagattg tgttcgcttg caatctgggc tccgagcatc tctccggcaa tgaagaggtg
     1261 tctcaaatag gccttggtgt atctcctgtc aacaaaagat gtaccatcag gatcaatggg
     1321 agagaagtcc tctgcccatt tgcggttgcg gatgttgatg atgccctcgc cggtaaagag
     1381 catcccgttg cgtccgttgc gggttggcat tacacagtca aacatgtcga taccacgggc
     1441 tattgactca aggatattgg ccggagtgcc cactcccatc agatagcgcg gcttatcctg
     1501 aggcaggata ggatgtacct cctctatcat ttggtacatc agttcggtag gttcacctac
     1561 cgagagtcct cctatggcat agccctccct gtcgagggcg gtaagctcct cagcagcttt
     1621 acgcctcagg tcgggataga tacacccttg aactatcggg aagaaggcct gatggtggcc
     1681 gtagaggggt tccgtactgt cgaatcgtgc gatgcatcgc ttgagccact tcatggtaag
     1741 atcgagagac tcagccgcgt acctacagtc ggcatcgccc gggcagcact cgtcgagcgc
     1801 catgatgata tcggcaccta tgatgcgttg gatatcgaca ttgttttcag gggtgaaaag
     1861 gtgtttggaa ccatctatgt gagattgaaa ctcgtatcct tcgtcagtaa gtttgcggca
     1921 ctgagccagc gaaaaaacct gaaatccgcc gctgtcggtc aggattggct tctcccagga
     1981 gatgaaattg tgcagtccgc ccgcctgccg gagaatatcc ggtccgggac gaagatagag
     2041 atggtaggta ttgcccagaa taatctgtgc gccgatatcc tcttcaagat cgcggtgata
     2101 aactccttta acggaaccta ccgtacccac gg
//
LOCUS       DAOSIE010000252         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_45177_length_2117_cov_5.588264, whole genome shotgun sequence.
ACCESSION   DAOSIE010000252 DAOSIE010000000
VERSION     DAOSIE010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45177_length_2117_cov_5.588264"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            644..1918
                     /locus_tag="PLI69_06620"
     CDS             644..1918
                     /locus_tag="PLI69_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42193.1"
                     /translation="MSIHSDINEEFVVGGQPVQISEQNDISVAVIATPDKDTVRYTVD
                     ITNYRNEPVFLDVDAIEVQQGRSELHHWKPLATYSAIGYAKRLKSINTFNQIVYGVAA
                     GISSASAFYSTSYTTGYGYGAYTTTYNPSAGYASSGSYLLMADIDDYLSKDHARSIDR
                     AYLKSSQIIPGSNQIGLFFTKDGSQPDYKLIVPIEDEVFEFQFRRSDYKEVLNPWLDR
                     SKPRHSLLFSYTPRSPRLGFYYKYSDDAAWGWYFGATLASNNDWPTFDLWATTSSYDG
                     GQTLLRPNAWTQDPDPSDSLRSPSDYDWFFDHKKGSDFFNEYFSMYVGFTRKLIPYTW
                     LLGGIGFGVHDGEYYQGTVFYKPKGADSSEYKSYGSGWIYNSYDSMLLIVPQFGFGWI
                     FNNIDLTLFASVPFADFTKSKFYVEFLVGFAI"
BASE COUNT          559 a          447 c          506 g          605 t
ORIGIN      
        1 atcgcacgat gggcgtggaa ggctcaaagc cgaacgacgg catctgatac ccaataaacg
       61 gaggagaaga gaggaccaag cttgctcgat gtcaggcctg tgagtatggc ttaagtggtt
      121 accgacaata attataaagg gattgattgt gcatttctat gttattcctc atgtaatgtt
      181 tgaatcgctt tgaccacgta ggcaaatgct tcaatattga gttttctgag agcagagagt
      241 tttgcatacg atgagaagag aggggaggcg tctctttgtt tgctggggta gccgagagat
      301 atttgaagat ggtttatgga actctccagg aaattgtggt gctgtgatgt tgtgaggtgt
      361 ccgcatcaag ctggaatagg caatcatagt caaaaatgat caatctcatc cttaaatgac
      421 cttttcaaaa gatgatgtga ctgataagct cctcctgatt gcttgtcagg gtgatttttt
      481 ttaacttgag cattcacaca acaactttgt tttgcatctt ggagaacatc acatgcgtgt
      541 cagcacctca gcctttgcag gaagacgacc gaaatctctg gtattgcttg taactaccat
      601 ctttcttctt tttcttatca gttgtgaatc aacccatcat ttaatgagta tacatagtga
      661 tataaacgaa gaattcgtgg ttggaggaca accggtccag attagcgaac agaacgatat
      721 tagtgtggcg gtaatcgcta cgcctgacaa ggatacagtg cggtatactg ttgatattac
      781 caactatcga aacgaaccgg tttttcttga tgtcgacgca attgaggtgc aacaaggaag
      841 atctgaattg catcattgga agcctttggc cacctattcg gccattggat acgcaaaacg
      901 gctgaaatcc atcaacacgt tcaatcagat tgtatacggt gtagctgccg gcatcagtag
      961 cgcaagtgct ttctactcca cctcctatac gactggctat gggtatggtg cctacacgac
     1021 aacgtataat cctagcgcag gctatgcaag ttcagggtcc tatctattga tggctgatat
     1081 cgatgattat ctgtccaaag accatgcacg ttcaatcgat cgggcgtatc tcaaaagttc
     1141 tcagataatt ccaggatcaa atcagatcgg attattcttt acaaaggatg ggagccaacc
     1201 agactacaaa ctcatcgtac ccattgaaga tgaggtcttt gagtttcaat tcaggcgtag
     1261 tgattataag gaggtactca acccatggct cgatcgtagc aaaccacgcc attcacttct
     1321 gttctcatat accccccgat caccacgcct cggtttctac tacaagtact ctgatgacgc
     1381 tgcttgggga tggtattttg gtgcaaccct tgcatcaaac aatgattggc ctacgtttga
     1441 tctttgggca actacatctt catatgacgg aggtcaaaca ctcctgcgcc caaatgcttg
     1501 gacgcaggat cctgatccga gcgatagtct gcgcagtcct tctgattacg attggttctt
     1561 tgaccataaa aaaggaagtg atttcttcaa tgagtatttt tccatgtatg tggggtttac
     1621 tcgaaagctc ataccgtaca catggttgct cggaggtatt ggttttggag ttcatgatgg
     1681 agagtactat caaggcacag tcttttacaa accaaaagga gctgattcca gtgaatataa
     1741 gagctatggc tccgggtgga tttacaatag ttatgactca atgttgctca ttgttcctca
     1801 atttggattc ggatggattt tcaacaatat tgatcttact ctttttgcgt cagttccatt
     1861 cgccgatttc acaaaatcca agttctatgt ggaattcttg gttggctttg ctatttgatt
     1921 gtctcgctca gtacatatat cgaaccacta ccgccgcaat acccgtacca gatacccatt
     1981 gaggtaggag gactcgggga aggagaggcg gatcgggtgg tcctcgtcct gtgaaaaggc
     2041 gtggaggatc tggatctcca cgtgggcgtc ggtggcgctg aaggcgagca tcaggcgcag
     2101 catctcggcg gtgacat
//
LOCUS       DAOSIE010000253         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_45179_length_2117_cov_5.301649, whole genome shotgun sequence.
ACCESSION   DAOSIE010000253 DAOSIE010000000
VERSION     DAOSIE010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45179_length_2117_cov_5.301649"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1398
                     /locus_tag="PLI69_06625"
     CDS             91..1398
                     /locus_tag="PLI69_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG42194.1"
                     /translation="MKISIINRNILRLALPSIMANITVPLVGIVDIAIAGRLGDAASI
                     GGIAIGSMLFDLLYWNMGFLRVGTGGITAQAYGRRDFKEAMDTFTRGIATALTVALLI
                     WIIQWVYIEAAFALVVSSPEVESIAREYFFIRVWAAPATLSLMVFKGWFIGMQNTVLP
                     MAVDVTVNVVNVVVSYILAIHTPMGVAGVAAGTVVAQYSGLLLAVVLMCYNFREHFHL
                     ISVRASIRLNKMKEFFSINGNLFIRSLGMLVIYIGFTSMAAKYGDTLLAVSSVMMKLL
                     LLFSYFIDGFAYAGEALVGRFIGEKNENYLKGTIRWIFIWSFGISALSTLIYALFGKP
                     MFRLMTDTPDVVEASTPFLFWLLLMPVVSCIAFTWDGIFIGATATKYLRNSMIWSAVL
                     FIVTYIAFRPLMGIQSLYLAYFVHLLVRSLYLTLVRKSALMIK"
     gene            1424..>2117
                     /locus_tag="PLI69_06630"
     CDS             1424..>2117
                     /locus_tag="PLI69_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOG42195.1"
                     /translation="MKIAFLSTFYPFRGGIAQFNANLYEELGKEHQVKAFTFKRQYPS
                     LLFPGKTQYVTEQDDAKVIESIAVLDSINPFSYFSTAKKIAGLEPDVVIMRYWISFLA
                     PALGTVARLLRRKGIKVITITDNIIPHERKFFDKPFAKWFLKPINGCIAMSSSVLEDM
                     LSLTPDKPYILKPHPPYDHFGAKMEKNEACERLGIKPDKKNLLFFGLIRDYKGLDLLI
                     EAMGHLDDSYRVI"
BASE COUNT          546 a          452 c          513 g          606 t
ORIGIN      
        1 tgccgttaaa gcggcacttg aagagggaag tatttcatac gagagataca attcctatct
       61 cggaatgctt gaagaagaga ccaaatatcg atgaaaatct ccatcatcaa ccgaaatatc
      121 ctgcgtctgg cactacccag cattatggcc aatattaccg tacccctggt gggtatagta
      181 gatattgcca tagccggccg tctgggagat gcagccagta tcggaggcat agccatcggc
      241 tcgatgctct ttgatctgct ctactggaat atgggatttt tgagggtagg caccggtggt
      301 ataacagcgc aggcttacgg caggagagat ttcaaggagg cgatggatac ctttaccaga
      361 ggcattgcta ctgccctgac tgtagcactg ttgatctgga ttatccagtg ggtgtatatt
      421 gaagcggcct ttgcgcttgt ggttagctca cccgaggtgg agagtattgc aagggagtat
      481 tttttcatca gggtatgggc agcaccggct acgctctcgc ttatggtatt caaggggtgg
      541 ttcatcggca tgcagaacac tgtgctacct atggccgtag atgttaccgt caatgtagtc
      601 aatgtggtgg taagttatat tttagccatc catactccga tgggagtagc gggcgtagct
      661 gccggaacgg tggtagcgca gtacagcgga cttttactgg ccgttgtact gatgtgctac
      721 aatttcaggg aacatttcca tctgatatcg gtgcgggcga gcatcaggtt gaacaagatg
      781 aaggaatttt tctcaatcaa cggaaacctg ttcatccgtt cgctcggaat gctggtaata
      841 tatataggat ttacctcaat ggcggctaaa tatggcgata ctttactggc agtaagttcg
      901 gttatgatga agctcctgct gctattttcc tattttatcg atggtttcgc ctatgcgggt
      961 gaagcacttg tggggcgttt tattggagag aaaaatgaaa attatttgaa agggacaata
     1021 cgctggattt ttatatggag cttcggtata agtgctctat ccactcttat ctatgctctt
     1081 ttcggcaagc cgatgttccg gcttatgacc gacactcctg atgttgtgga ggcttctact
     1141 ccatttttat tctggttgtt gctcatgccc gtagtgagct gcatcgcttt tacctgggac
     1201 gggatcttta ttggagccac ggcaaccaaa tacctgcgca atagtatgat ctggtctgcc
     1261 gtgctattta tagttaccta catcgccttc cggcccctaa tgggcatcca gtcgctctat
     1321 ctcgcctatt tcgtccatct gctggtacgc agtctctacc tcactctcgt gagaaaaagt
     1381 gctttgatga taaagtgata atatttatta tctttgtgtt attatgaaaa tagcatttct
     1441 ctccactttt taccccttca ggggaggcat cgcccaattc aatgcaaatc tttacgaaga
     1501 gctgggtaag gagcatcaag tcaaggcttt cacatttaag agacaatatc cttcgcttct
     1561 tttcccgggt aagactcaat atgtaactga gcaggacgat gcaaaagtaa tagagagtat
     1621 tgctgtattg gactcaataa atcccttctc ctatttcagc accgcaaaga agatagccgg
     1681 actggagccg gatgtggtca taatgagata ctggatatcc tttcttgcac ccgcattggg
     1741 aacggtagcc agactgctta ggagaaaggg tatcaaagtg attacaatta ctgataatat
     1801 tatcccacac gagagaaaat tcttcgataa accctttgca aaatggtttc taaagccgat
     1861 taacggatgt atcgccatga gcagctcagt actcgaagat atgctgagtc ttactcccga
     1921 taagccctat attctcaagc ctcatccacc ctacgatcat tttggagcaa aaatggagaa
     1981 aaatgaggcc tgcgaaagac tggggatcaa gccggacaaa aagaacctgc tttttttcgg
     2041 acttatccgt gattataagg gacttgatct gcttattgaa gcgatgggac acctcgatga
     2101 tagttacaga gtgatca
//
LOCUS       DAOSIE010000254         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_45359_length_2111_cov_5.442121, whole genome shotgun sequence.
ACCESSION   DAOSIE010000254 DAOSIE010000000
VERSION     DAOSIE010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45359_length_2111_cov_5.442121"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..652
                     /gene="ruvA"
                     /locus_tag="PLI69_06635"
     CDS             56..652
                     /gene="ruvA"
                     /locus_tag="PLI69_06635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOG42196.1"
                     /translation="MYDYIKGILIELNPADVTIEAGGIGYHILISLTTYSSLQRGSKE
                     SVKLYLHQHLREDEELLFGFFDKEERTMFRHLISVSGIGPGTARMMLSSMTPDEVFSS
                     IVTQDVNRIKSVKGIGIKTAQRVILELQDKVGKGISPTGFPFDGAAAPSALLQEASTA
                     LVLLGFSKPSVEKALDKLLKESPSISLEELIKKALKLL"
     gene            743..1117
                     /gene="gcvH"
                     /locus_tag="PLI69_06640"
     CDS             743..1117
                     /gene="gcvH"
                     /locus_tag="PLI69_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOG42197.1"
                     /translation="MNIPAELKYSKDHEWIKIEGDTAIVGITDFAQSELGDIVFVDIQ
                     TEGEFLEKDEIFGAIEAVKTVADALMPLSGEIVEVNPELEGAPELVNNDPYGAGWMIK
                     IKYSVASEIDTLLSAKQYEEII"
     gene            1230..>2111
                     /locus_tag="PLI69_06645"
     CDS             1230..>2111
                     /locus_tag="PLI69_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG42198.1"
                     /translation="MTITRTFDLLSNLKASFPPKDDMLARKVNGRWIKYSIDDYIEHS
                     HNVAYGLLALGYKAGTKIITICSNRPEWNFVDMGSNLAHMIHVPVYSTLSKKDFSYIF
                     DHSDAEIIIVGNKNLYNKIAPSVAEIKRNIKVILMDDSEDIFCMSKLEALGRENKEQF
                     APIVERNKEEISPDECCSIVYTSGTTGLPKGVMLSHRNLTFNSHGHAVRQIWNYNHKM
                     LSFLPLCHVYERTMNYEFQELAISIYYAESLSTIAADLLDSRADGFCSVPRVLEIMYG
                     KLEAAGKRLKGIKKLIYN"
BASE COUNT          604 a          444 c          467 g          596 t
ORIGIN      
        1 ctttggtttt tggcctaaaa agcatatttt tacatgaaaa ttaaactaaa agagaatgta
       61 cgactatatc aaaggcatac ttatcgaact caatcccgcg gatgttacta tagaggcggg
      121 cggcatcgga tatcacatat taatttctct cacaacctac tccagcctcc agagaggaag
      181 taaggagagc gttaaactct accttcacca gcatcttcgt gaagatgaag agctgctttt
      241 cggctttttt gacaaagagg agcgcaccat gttccgtcat ctgatctcag taagcggcat
      301 cggtcccggt acggcacgta tgatgctctc cagcatgaca ccggatgagg ttttcagctc
      361 tattgtcact caggatgtca accgcatcaa gagcgttaaa ggtattggta tcaagactgc
      421 acaacgagtg attctggagc ttcaggataa ggtcggcaaa ggtatttccc ccacaggatt
      481 cccctttgat ggtgcagctg ctccttctgc cctacttcag gaagcatcca ctgcgcttgt
      541 cctcctgggg ttcagcaaac ccagtgttga aaaggctctg gataagctct taaaagagag
      601 cccttccatc tctcttgagg aattgataaa aaaagcactc aaactactct gattttgagt
      661 tttttttgtt ggtttcaaat attaatgcta tatttgtaca atcatttttg gaatcagttt
      721 taacatttta atcggtttta taatgaacat tcctgcagaa ctcaagtata gcaaagatca
      781 cgagtggatt aagatcgaag gagacacagc catagtaggt atcaccgatt ttgcacagag
      841 cgaacttggt gacatcgtgt ttgtcgatat ccagacagag ggtgaatttt tggaaaagga
      901 tgaaatattc ggtgcgattg aagctgtaaa gacagttgct gacgctttaa tgcctctttc
      961 aggagagatc gttgaggtta atcctgaact tgaaggagct cccgagctcg tcaataacga
     1021 tccttatgga gcagggtgga tgattaagat caagtattcc gtcgcttcgg aaattgatac
     1081 ccttctatct gcaaagcagt atgaagaaat aatctgattt gtaaatcaaa tgtgatgtgt
     1141 tgagggaggt ccgagcgagg gcctcccttg tttttatttt tatttttcgg ggttaattat
     1201 taactttgga gttctaactt ttttaaaata tgacaattac ccgcacattt gacttgcttt
     1261 ccaatttgaa agcctctttt cctcccaagg atgatatgct ggctcgtaaa gtaaacggcc
     1321 ggtggattaa atatagcatt gatgactaca tagaacactc acataatgtg gcctatggtc
     1381 ttctggcact cggttacaag gccggtacaa agataattac catctgtagc aatcgtcccg
     1441 agtggaattt tgtggatatg gggtcaaatc tcgcccacat gatacatgtg ccggtgtaca
     1501 gtacgcttag caaaaaggat ttctcgtaca ttttcgacca ttcggacgct gaaatcatta
     1561 tcgttggcaa caaaaacctc tacaataaaa tcgctccaag cgttgcggag ataaagcgca
     1621 acatcaaggt aatactgatg gatgactctg aggatatttt ctgtatgtcg aagcttgagg
     1681 cactcggacg cgagaataaa gaacaatttg ctcctattgt tgagcgaaac aaagaagaaa
     1741 tatctcctga cgaatgctgt agtatagtct atacctcagg caccacaggt ttgccgaaag
     1801 gcgttatgct ctcccaccgt aacttaacct tcaactccca tggtcatgca gtgcgccaga
     1861 tatggaatta caatcataaa atgctcagct ttctccctct ctgccacgtg tatgagcgca
     1921 caatgaacta tgagttccag gaactggcca taagtattta ttacgccgaa tctctcagta
     1981 ctatagcagc agacctccta gatagccggg cagacggatt ttgctctgtt ccacgcgtac
     2041 tggagataat gtacggcaag cttgaggcgg ccggtaagag gttgaaaggc ataaagaagc
     2101 ttatatataa c
//
LOCUS       DAOSIE010000255         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_45516_length_2106_cov_2.784008, whole genome shotgun sequence.
ACCESSION   DAOSIE010000255 DAOSIE010000000
VERSION     DAOSIE010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45516_length_2106_cov_2.784008"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="PLI69_06650"
     CDS             <1..1360
                     /locus_tag="PLI69_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42199.1"
                     /translation="NNMTFKEIIDSPCGIRYCFDLLELQSGYARKYLLDSSMMTSAEE
                     IVEAYTYLDKFTTLLEREPSLIQNIQFRLQGLRNISNTLISLEEGSTLDDIEFFEIKH
                     LALLGDQVQQLFSKAERSNEWRVFDLDNVIRILDPDGLKIGTFYVYDSYSMELRKLRR
                     ELDSDPDNEELKDSLANEEERIKRVLCNLLRPYATELTNLLHALAELDILISKAIQRK
                     KESFTIPTVGTGSSFEGLFHPEVKSILAQKGKIFQKVSFRYNPGEPGIIIGANMGGKT
                     VALKSLCLCQYLFQFGFAVPAERAEMVPVENIVFCFGDDQDQQKGLSSFAAEILRIDR
                     MIEMVESGAKTLALVDEPARTTNPTEGSALVSSLLRILSNYPNLSLVLTTHYNVDSSA
                     RCWRVKGLVEDEKGYERLKMDYSLTESPDNEVPHEALNIARKLGVSPRWLDEATKILD
                     TQ"
     gene            1506..>2106
                     /locus_tag="PLI69_06655"
     CDS             1506..>2106
                     /locus_tag="PLI69_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOG42200.1"
                     /translation="MQSKLGLDFKKVDYARSLAKNIADDVQTFVESYTTVAVERTLCR
                     LLGIDGVDENQVPLPNVVVDELKGKGVLNQGVLFYIGNAMVQTGLSPQQIAEGVSSGT
                     IDLVRIEVVDKKKIDEILQPIINGSIDRIRARRERRNHYLNTIGEGPKPYIYVIVATG
                     NIYEDVVQAEAAARQGADIIAVIRTTGQSLLDYVPYGATT"
BASE COUNT          619 a          465 c          471 g          551 t
ORIGIN      
        1 gaataatatg acatttaaag agataataga ttccccttgc ggcattcgat attgctttga
       61 tttgcttgaa ctgcaatccg gctatgcccg caaatatctc ctcgacagca gtatgatgac
      121 aagtgccgaa gagatagtgg aagcgtatac ctatcttgat aaatttacca ctctgcttga
      181 aagagaacct tccctgatac agaatattca attcaggctt cagggattgc gcaacatctc
      241 caatactctc atctcccttg aggagggttc cacgctggat gacatagaat tttttgaaat
      301 aaagcatctt gctttgttgg gagatcaggt gcaacaacta ttctccaagg cggagcgctc
      361 taatgagtgg agggttttcg accttgacaa tgtgatccgt attttggatc cggatggcct
      421 taaaataggc actttctatg tatatgactc ctattcgatg gaattacgca agctcagaag
      481 ggagttggat agtgatccgg ataatgagga gcttaaagat agcctggcca atgaggagga
      541 gaggataaaa agagttcttt gtaacctact cagaccctat gcaaccgaac taacaaactt
      601 gttacatgct cttgccgagc tggatatcct catttccaaa gcaatacagc gcaaaaagga
      661 gtcatttaca ataccaacgg tagggaccgg ttcctccttt gagggtctgt ttcatccgga
      721 ggttaaatcc atccttgcac agaagggaaa aatcttccag aaagtctctt tccgatataa
      781 tcccggggag cccggtatca taataggagc caacatgggt ggtaagaccg tagctttgaa
      841 gagtctatgc ctctgccagt atctatttca gttcggtttt gccgttcctg cggagagggc
      901 tgagatggtg cctgtagaaa atatagtttt ctgttttggt gacgatcagg accagcagaa
      961 gggactctct tcttttgcag cggagatttt gcgtatagat cggatgattg aaatggtgga
     1021 gagcggtgcc aaaacgcttg ctctcgttga cgaaccggcc cgtaccacca accctaccga
     1081 aggtagtgca ctggtctctt ctctactccg aatactcagc aattacccta atctctccct
     1141 ggtactaact actcactaca atgtagatag tagtgcaagg tgctggagag ttaaagggct
     1201 ggtagaggat gagaagggct atgaacggct caaaatggac tactctttaa ctgagtctcc
     1261 cgacaacgaa gtaccccacg aggcgcttaa catagctcgc aagcttggag taagtccccg
     1321 atggctcgat gaagcaacaa aaatactcga cacacaataa actacacact acacactaaa
     1381 cactatttaa ttactacata ctacacacta cacactacat actacatact acatactaca
     1441 tactacacac tacaaactat ttaattacta cacactatta actacaaact acatactatt
     1501 atacgatgca aagcaaatta ggattggatt tcaagaaagt cgactacgca cgctctctgg
     1561 caaaaaatat agctgatgac gtgcagactt tcgtagagtc ttataccacg gtagccgtgg
     1621 aaaggactct atgccgactt ttggggattg acggtgtcga tgaaaatcaa gtaccgctcc
     1681 caaatgtcgt tgtggacgaa ttaaagggca aaggtgtttt gaatcaaggc gtgcttttct
     1741 atattggcaa tgcaatggtt cagacaggac tttcacctca acagattgca gaaggtgttt
     1801 cgtccggaac cattgacctt gtaagaatag aagtagttga taaaaagaag attgatgaaa
     1861 tccttcagcc gataatcaat ggttcgatag atcgcatccg tgccagacga gagcgtagga
     1921 atcattatct aaatactatc ggagaaggcc ccaaacccta tatctatgtg attgtagcca
     1981 ccggtaatat ctatgaagac gtggttcagg ctgaggctgc tgcccgtcag ggagcagaca
     2041 ttatagccgt gatccgtact accggtcagt cgcttctcga ctatgtccct tatggggcca
     2101 ctactg
//
LOCUS       DAOSIE010000256         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_45871_length_2094_cov_4.188818, whole genome shotgun sequence.
ACCESSION   DAOSIE010000256 DAOSIE010000000
VERSION     DAOSIE010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45871_length_2094_cov_4.188818"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1421)
                     /locus_tag="PLI69_06660"
     CDS             complement(9..1421)
                     /locus_tag="PLI69_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG42201.1"
                     /translation="MAAEEYLIEKIGEKLGYEPTTCQQKLIASLARFTVEYDTCDLML
                     VSGYAGTGKTSAIAAYVKTLKEHDHRFVLLAPTGRAAKVLAGFTQEQAFTIHKHIYRQ
                     KSINHGFGEFELDINKHKETIFIVDEASLISTSSAQQNIFGSGDLLSDLIAYLRAGVD
                     NKLILIGDKGQLPPIGLTYSPALDDSYMGRYGKVITSELTTVVRQAVDSGILSNATAI
                     RLLLGSSGPMIPQLRTKGFEDVIRIHGGELIESLSDMFDKYGTDEVVVLCRSNKRANR
                     YNMGIRSSVLFLEERLTRGDKLMVVKNCYQFLDDVPELDFIANGDVAELIRIYGYEER
                     YGLNFAEAVLSFPDYNDVEITAKIILDTLQSEAPALSEEQQRVLYEGVNEDYAHIKGK
                     RKRYASVREDKYFNALQIKYAAAITGHKSQGGQWKCVFIDNPFWKEPVEDDLKWLYTA
                     ITRGVERVYFVNFKDDFFID"
     gene            complement(1421..>2094)
                     /locus_tag="PLI69_06665"
     CDS             complement(1421..>2094)
                     /locus_tag="PLI69_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOG42202.1"
                     /translation="TRSAKIFKDPVTRFYLMTILISTVIVAITLLLEGVEQGIFHALR
                     NGAFAVVSTISTTGFSISDTSGWPILAIMVILYVSVQCGCSGSTCGGVRSDRMWLILK
                     GVRAQLIKMAHPNAVVQVKSGGQVIEKDLISSVHTFLFIYLVLTFLGSLFYAMTGMSM
                     MESVSSSISMMGNMGLAFGENNSFYNFANAPQISKIVMGIQMIVGRLSLYPVLLIFTL
                     FRKRF"
BASE COUNT          601 a          506 c          424 g          563 t
ORIGIN      
        1 tattcattct aatctataaa aaaatcatct ttaaaattaa caaaataaac tctctcaact
       61 cccctggtaa tagcagtata aagccatttc agatcatcct cgacaggctc tttccagaaa
      121 ggattgtcaa tgaaaacgca tttccactgg cctccctgcg atttgtgacc tgtaatggct
      181 gccgcatact tgatctgaag agcattaaaa tatttgtcct cacgcacgga agcgtatctc
      241 ttacgcttcc cttttatgtg agcataatcc tcattgacac cttcataaag aactctctgt
      301 tgctcttcgc tcaaagccgg agcttccgac tggagcgtat caaggataat tttcgccgta
      361 atctccacat cattgtagtc cgggaatgac aatactgcct ctgcaaaatt caatccatac
      421 ctctcttcat atccatatat tctaataagt tcggcaacat cgccgtttgc aatgaaatcg
      481 agttcaggca catcatccag aaactgatag caatttttaa ccaccatcag tttgtcaccg
      541 cgcgtgagac gctcctcgag aaaaagtaca cttgagcgta tgcccatatt gtagcggtta
      601 gcacgtttat tggacctgca aagtaccacc acttcatctg tgccatattt gtcaaacata
      661 tcggagagcg actctatgag ttcacctcca tgaattctaa taacatcttc aaaccccttt
      721 gtgcgtaatt gtggtatcat cggaccactg ctacccaaaa gtaacctgat ggcagtagca
      781 ttgctcaaaa ttcccgaatc tacggcttgt cgcaccacag tagtaagctc agaagtaatt
      841 actttcccgt aacgccccat ataagagtca tccagcgccg gactgtaggt aagccctata
      901 ggtggcaact gtcctttgtc tccaattagg ataagtttgt tatcgactcc ggcacgcaag
      961 taagctatca ggtcggatag cagatcgccg cttccgaata tgttttgttg ggcgctggaa
     1021 gttgagatca gtgaggcctc atccactatg aagatagtct ccttgtgctt atttatatcc
     1081 agttcaaact cgccgaaccc atggttaatg gatttttggc gataaatatg tttatgtatg
     1141 gtaaatgcct gttcctgagt aaaacctgcc agcacctttg ctgcacggcc tgtgggagcc
     1201 aggagcacaa aacggtggtc gtgctctttg agtgtcttga cgtatgcggc gattgcggag
     1261 gttttgccgg taccggcata tccactgaca agcatcagat cgcaagtgtc gtattcgaca
     1321 gtaaaacgag ccagggaggc tattaatttc tgctgacaag tagtgggttc ataccccagt
     1381 ttctccccta ttttctctat gagatactcc tcagctgcca ttaaaatctt ttcctgaaga
     1441 gggtgaatat aagcaataca gggtagaggc taagacggcc gactatcatc tgaataccca
     1501 ttacaatttt tgaaatctgt ggagcgttgg caaaattata aaaagaatta ttctcaccaa
     1561 aggcaaggcc catattgccc atcatggata ttgaggaact caccgactcc atcatactca
     1621 taccggtcat agcatagaac agtgagccga ggaaggttaa aaccagatat atgaatagaa
     1681 atgtgtgtac actcgaaatc agatcctttt caatcacctg gccaccggac ttaacctgta
     1741 caactgcatt tggatgggcc atcttgataa gttgagctct tacacccttt agtatcaacc
     1801 acatcctgtc tgaacgaaca ccaccgcagg tagatccgga gcaaccgcac tggaccgaaa
     1861 catacaagat aaccattatg gcaagtatag gccatccgga ggtatcgcta atagaaaaac
     1921 ctgtggtact gatagtagag accaccgcga aagcaccatt gcgcaatgcg tggaaaatgc
     1981 cttgttcaac tccctccaac aaaagagtaa tggccactat aaccgtggaa ataagtatgg
     2041 tcatcagata aaacctggtg accggatcct taaatatttt ggccgacctg gttg
//
LOCUS       DAOSIE010000257         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_45984_length_2091_cov_3.826621, whole genome shotgun sequence.
ACCESSION   DAOSIE010000257 DAOSIE010000000
VERSION     DAOSIE010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45984_length_2091_cov_3.826621"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PLI69_06670"
     CDS             complement(<1..904)
                     /locus_tag="PLI69_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOG42203.1"
                     /translation="MSDTLFTPYKIGPIELPNRTIRSAAFEGMCPGHRPSQELFDYHT
                     AVARGGVGMTTIAYASVSRSGLSFERQLWMREEIIPELKALTEAIHREGALASIQLGH
                     CGNISHRSICKCWPKGASSGFNIYSPTIVHGMRVEEIDEIVEDFGKAVHLAIKAGFDA
                     VEIHAGHGYLISQFLSPYTNHRKDEYGGSLENRMRFMERVMKEVMAAASDKVAVFVKI
                     NTRDGFKGGMEIDDCIRVALRLQELGAHALVLSGGFVSRMPMYVMGGAFPVKAIAHYM
                     PPKHWWLKLGVRLFGHIVAPSVPFK"
     gene            complement(897..1517)
                     /locus_tag="PLI69_06675"
     CDS             complement(897..1517)
                     /locus_tag="PLI69_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42204.1"
                     /translation="MKRIGIIILAAAAVISSCTGKKSVSSLEKAIEEEIINTLDGAYD
                     FSIIELEKLDSTLFLTEFDRRINVFEMRIEANKKFLDDYFRRGLKKNAALKYDEIYKD
                     YDILEALHAVKERMGDSLYMVAYYDYRVKAQIKTGGIVRKTIGRVLKRNAQVLDDIYL
                     AITPDNRVVSMNTDRKKLHQSTGLVIPGYSEIIKSESTSTEVPEDE"
     gene            complement(1544..>2091)
                     /locus_tag="PLI69_06680"
     CDS             complement(1544..>2091)
                     /locus_tag="PLI69_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOG42205.1"
                     /translation="IIVITLLFLTHYLQRTFIFPLLIRGESKMPLSIIALGMTFNFTN
                     AYMQGYWLYYLSPDDMYTVEWLKTPQFIMGTMIFIVGMVINLHSDYIIRHLRKPGDNA
                     HYIPMGGMFKYVSSANYFGEILEWTGFAILTWSLPGVVFLLWTCANLVPRANSLYLKY
                     TEKFGEEFTSLKRKRVFPFLY"
BASE COUNT          518 a          501 c          472 g          600 t
ORIGIN      
        1 ccttgaaggg aacagaagga gcaactatat ggccgaatag gcgaactccg agtttgagcc
       61 accaatgctt cgggggcata tagtgggcga ttgccttaac agggaatgcg ccgcccataa
      121 cgtacatcgg catcctgctt acaaatccgc cgctgagtac cagcgcatga gcgcctagct
      181 cctgaagccg gagcgccaca cggatacaat catcgatctc cattccaccc ttgaaaccat
      241 ctcgggtatt gattttgaca aataccgcga ccttatcact tgctgctgcc ataacttctt
      301 tcattacccg ctccatgaat cgcatcctgt tttccagaga gcctccatat tcatccttac
      361 ggtggtttgt gtatggtgat agaaattggg atatgaggta gccgtgtccc gcgtggattt
      421 ctacggcatc aaatccggct tttatggcca aatgtaccgc tttaccgaaa tcctcaacta
      481 tctcatcaat ttcctccact ctcatgccat gtacaatggt gggggagtag atgttgaaac
      541 cgctgctggc acccttcggc caacacttgc aaatggatct gtgagatatg ttgccacagt
      601 gtccgagctg gattgaggcg agtgcacctt ctctatggat tgcctctgtg agggctttca
      661 gttccggtat tatctcttct ctcatccaga gctgtcgctc gaaagagagt ccgctccggg
      721 ataccgaagc gtaggcgata gtggtcatac caacacctcc acgtgccacc gcagtgtggt
      781 aatcaaatag ttcctgcgaa ggtctgtggc cgggacacat cccttcaaat gcggccgaac
      841 gaatggtgcg gttcggaagt tctatcgggc ctattttgta aggtgtgaag agcgtatcac
      901 tcatcttccg gtacttctgt tgaagttgac tctgatttga taatctcact atatcccggg
      961 atgacgagcc ccgtggactg atgaagtttt ttacggtccg tgttcatgga gactactctg
     1021 ttgtccggag tgatggctag gtagatatca tccaacacct gtgcatttct cttcaatact
     1081 cttcctatcg ttttcctcac tatacccccc gttttaatct gagcttttac gcggtagtcg
     1141 tagtatgcca ccatatagag ggagtcgccc atcctctcct ttactgcatg tagtgcctca
     1201 aggatatcat aatccttgta tatctcatca tacttgaggg ctgcattctt tttgagtccc
     1261 ctacggaaat aatcatcgag aaattttttg ttagcttcaa tcctcatttc gaacacatta
     1321 attctccgat caaattccgt taagaagaga gtggaatcca gcttttcaag ttctattatg
     1381 gaaaaatcgt atgccccgtc gagcgtatta atgatttcct cttcgattgc cttttcgagg
     1441 ctggatactg atttcttacc tgtgcaggag cttattaccg ccgctgccgc taaaatgata
     1501 atgcctattc gtttcatatc actattattt tatgataaat atttcaatat aaaaaaggga
     1561 acacacgttt gcgttttagg gaagtaaatt cttcgccaaa cttctccgtg tactttaggt
     1621 agagggagtt tgccctcggt accagattgg cgcaggtcca tagtaggaat acgactccgg
     1681 gtagcgacca tgtaaggatt gcaaatcctg tccattccag tatctcgcca aaatagttgg
     1741 ctgatgaaac atacttgaac atgcctccca tcgggatata gtgggcattg tcgccgggct
     1801 ttcgcaggtg gcgtattatg tagtccgagt ggagattaat taccattccg actatgaaaa
     1861 tcattgttcc cataataaac tggggagtct ttagccattc caccgtatac atatcatccg
     1921 gagagagata atagagccaa tatccctgca tgtacgcgtt ggtgaaatta aatgtcattc
     1981 ccagggcgat gatactgagt ggcatcttac tctcacccct tatcagcagg ggaaatatga
     2041 aagtacgctg gaggtagtgt gtcaaaaaga gaagcgtaat gacaattatt g
//
LOCUS       DAOSIE010000258         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_46316_length_2080_cov_3.130864, whole genome shotgun sequence.
ACCESSION   DAOSIE010000258 DAOSIE010000000
VERSION     DAOSIE010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46316_length_2080_cov_3.130864"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLI69_06685"
     CDS             <1..246
                     /locus_tag="PLI69_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOG42206.1"
                     /translation="YRDMAALGGGLNFYGELPESYNIVINAENPLIKRIMGEKGTEAF
                     TADNILLKQVTDLALLANNMLKGKELSDFIKRSAELL"
     gene            complement(250..>2080)
                     /locus_tag="PLI69_06690"
     CDS             complement(250..>2080)
                     /locus_tag="PLI69_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HOG42207.1"
                     /translation="GARYWLQWAGFNDTIYSVNRNFTDYNDDYQSRGMWVNILTDGSY
                     LKPDRKGYNIPIDLSFAFHTDAGTTLTDSIIGTLAIYTRFSMDKDEYPYGVKRSIARD
                     YTDIVQTQIVDDIRATFEPQWSRRGLWDKSYSESRSPEVPAMLLELLSHQNLADMRYG
                     LDPAFRFVVARAIYKGMLKYLSWLRETPYIVQPLPVRAFSAEIGGSGGRYRAELKWEP
                     RVDRLESTATTRSYILYTRVDDGGFDNGLPVQGNSFNVNIEPGKIYSFKVTAVNEGGE
                     SFPSEILSVGLADSRSSRVVAKGKTVLVVNNFDRVAAPSSFATRDSSYAGFRDHIDRG
                     VPYLYDISHIGSQYEFRRTIPWMDDDAGGFGASHSDYAAKPFAGNSFDYPYYHGLALM
                     RAGYDFVSTSREALTSGVVDLEKYPILDLICGKQLTTLTGRDGASKLRFSVFPADLQE
                     TLREYAASGGNMLISGANIATDAWDQIYDYGIDSLMMADVIVPMRNFITGVLKYKWMT
                     NNATAGCDVKMVSNPFGLPLSSYSFHTKPNPYRYQVESPDGLVPADKNAYTIFRYSDN
                     NISAGVAYEGAYRVVSLGFPIETLQSQEQIDNLIKELMQFLDK"
BASE COUNT          562 a          485 c          511 g          522 t
ORIGIN      
        1 tatcgtgata tggctgcact cggtggagga ttaaatttct acggagagtt gcccgagtcc
       61 tacaacattg taatcaatgc agaaaatcct ctgataaagc gcataatggg agagaagggt
      121 actgaggcat tcactgccga caatatcctt ctgaaacagg ttacagacct cgctttgctc
      181 gccaataata tgcttaaagg caaagaactt agtgacttca taaagcgaag cgcagaatta
      241 ttgtaagaac tacttgtcaa ggaactgcat cagttccttg ataaggttat ctatctgctc
      301 ctgtgattgt agtgtctcta tgggaaagcc cagggaaact acacgataag cgccctcgta
      361 agccactccg gctgagatat tattgtcgga atacctgaaa attgtatatg cattcttgtc
      421 ggcagggaca agtccgtccg gactttctac ctgataacga taaggattgg gcttggtatg
      481 gaatgaatag gaggacaatg ggagtccgaa aggattggaa accatcttga catcacatcc
      541 tgcggtagca ttgttggtca tccatttgta tttaagtacc cctgtgatga aattgcgcat
      601 aggaactatt acgtcagcca tcattaagga gtcaatccca tagtcatata tctgatccca
      661 tgcatctgtg gcaatattgg cccccgaaat gagcatattc ccgccggagg cggcatattc
      721 tctcaaagtt tcctgtaggt cggccggaaa gacgctaaag cgcaatttac ttgcaccatc
      781 tctgccggtg agagtagtaa gctgcttgcc acatataaga tcgagtatgg gatatttctc
      841 aagatccaca acacctgagg tcaatgcctc gcgggaggtg gaaacaaagt cgtaacccgc
      901 cctcatcagc gccagcccgt gataataggg ataatcgaaa ctatttcctg caaagggctt
      961 tgccgcataa tcggaatggg aggctccaaa accgccggca tcatcatcca tccaggggat
     1021 tgtgcggcga aattcgtact gagagccaat atgagagata tcgtagagat aggggacccc
     1081 tctatcgata tgatccctga atcccgcgta agaagaatcg cgggtggcaa atgaggaggg
     1141 agcagcaacc ctgtcaaagt tattaaccac gagtactgtt ttacccttgg caaccactct
     1201 tgaggagcgt gagtctgcaa ggcccacgga aagtatctcg gagggaaaac tctcaccacc
     1261 ctcgttgacc gcagtcacct tgaaactata tatcttaccc ggctcaatat tgacgttgaa
     1321 ggaattgcct tgcacaggca gaccattgtc aaagcctcca tcgtctactc ttgtgtagag
     1381 aatataactg cgcgtggtcg cggtactttc caatctgtca acacgcggtt cccatttcag
     1441 ttctgccctg tatctaccgc cggaaccgcc tatctctgcc gaaaaagcgc gtacaggtag
     1501 tggttggaca atatagggtg tctctctaag ccaggaaagg tacttaagca tgcctttata
     1561 tatcgctctt gcaaccacaa atctgaatgc agggtcgagt ccgtaacgca tgtcggccag
     1621 attttggtgg gagaggagct ccagcagcat tgcaggtact tccggcgagc gtgactccga
     1681 gtaggacttg tcccatagtc cccgacgaga ccattggggt tcaaatgtag cccggatatc
     1741 atctacaatc tgagtttgta ctatgtcggt atagtctctg gcaatggacc ttttcactcc
     1801 ataagggtac tcatctttat ccatggagaa gcgggtgtag atggcaagcg taccaatgat
     1861 ggagtctgta agtgtggtac ctgcatcggt atgaaatgca aacgaaagat cgatggggat
     1921 gttgtaaccc ttgcgatcgg gcttcagata ggagccgtca gtaaggatat ttacccacat
     1981 tccgcgtgac tgataatcat cattgtaatc ggtaaagttg cggttgacgg aatagatggt
     2041 atcgttgaat cccgcccact gtaaccagta ccttgcaccc
//
LOCUS       DAOSIE010000259         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_46524_length_2073_cov_3.538157, whole genome shotgun sequence.
ACCESSION   DAOSIE010000259 DAOSIE010000000
VERSION     DAOSIE010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46524_length_2073_cov_3.538157"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLI69_06695"
     CDS             complement(<1..258)
                     /locus_tag="PLI69_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-5-alpha-steroid 4-dehydrogenase"
                     /protein_id="HOG42208.1"
                     /translation="MSIYNITLITMSALAVVVFVALFFLGAGYGFLRTPKWGPSIPNK
                     IGWILMEAPIFVTMLGLYLDSDRVGDPAIIVITLLFLTHYLQ"
     gene            complement(262..1008)
                     /locus_tag="PLI69_06700"
     CDS             complement(262..1008)
                     /locus_tag="PLI69_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOG42209.1"
                     /translation="MDIESRKNLYTEHFYIKSFETDPFKIFKLSSFLSVAQEIAGVSA
                     TRMGFGYDHLIKENNVWVLSRVKVKYLRPPRWREFVKMETWHKREEGLMALRDYELLT
                     EEGESIAVSTSSWLIMNMESRRLKRTSEILGEKGMHYMDRDAIVESCGKLTAPDNLQL
                     VYTKRVRISDIDYNYHANNARYADWIIDAFGMDFLKEHPLDEIQINYNSECTYDNIVE
                     IYRAPLEEGSFYVEGRSDGRNIFQSLISFK"
     gene            1077..1856
                     /locus_tag="PLI69_06705"
     CDS             1077..1856
                     /locus_tag="PLI69_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOG42210.1"
                     /translation="MKKPLLYIVLFVSCVLLTACESSAQNARHYTLQVEESLPHDVSA
                     YTQGLFFYEGELYESCGQYGESSFRKVELATGKVLQRINFDRKYFLEGACALKDRIYI
                     LTWQENTCFVYDIKTMSLIGSVRYRGEGWGLTTDGKYLIMSDGSSSLTFRNPDNFKVE
                     KQVTVTLNGKSLRYLNELEYINGEIWANVYGSDAIVTINPSSGAVTGMIDCRNLLPSA
                     LRKPSTDVLNGIAYNDRDGAIWLTGKYWPKIFRVTLVEKQK"
     regulatory      1892..1989
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          565 a          469 c          455 g          584 t
ORIGIN      
        1 ctgaaggtag tgtgtcagaa agagcagcgt aatgacaatt attgcaggat cacctacccg
       61 gtcactatcg agataaagcc ccaacatagt aacgaaaata ggtgcttcca tcaatatcca
      121 tcctatctta tttggaatgg aaggacccca cttgggagtc ctgagaaagc catatcctgc
      181 tcctaagaag aagagtgcca caaaaacgac tacggccagc gcactcatgg taattagggt
      241 tatattgtag atgctcatat attacttgaa tgaaatcagg gattggaaaa tattacgccc
      301 atcgctacga ccctctacat aaaatgaccc ctcctctaaa ggtgccctat atatctctac
      361 aatattgtca taagtgcatt cgctgttgta gtttatctga atttcatcca ggggatgttc
      421 tttaagaaaa tccattccga aagcatcaat tatccaatct gcatacctgg cattgtttgc
      481 gtggtagttg taatcaatat cggagatacg cacgcgcttg gtatagacaa gctgtaggtt
      541 gtccggcgca gtaagtttac cgcaactttc aacaatcgca tccctatcca tatagtgcat
      601 ccccttctct ccaaggatct ccgaagtcct tttcagacgg cggctctcca tattcataat
      661 gagccaggaa ctggtggaca ctgctatact ttctccttcc tccgtcagca gttcgtagtc
      721 tcgaagggcc atcagcccct cttctcgctt gtgccaggtc tccattttaa caaattctct
      781 ccagcgcggc gggcgcaggt atttaacctt gactctcgag agcacccaga cattgttctc
      841 ctttatcagg tgatcatagc caaatcccat tctggttgca cttacacctg caatttcctg
      901 agctacgctc agaaatgatg aaagtttgaa aatcttgaat gggtctgttt caaaactttt
      961 gatataaaaa tgctctgtat ataggttctt gcgactttct atatccatat cttatgttac
     1021 tttattttcc gattagactg ctaatgtacg aaaaaatatc tacttttgtc tgcgaaatga
     1081 aaaaacctct gttgtatatc gtgctttttg tctcctgtgt acttctcaca gcctgtgaga
     1141 gcagtgctca gaatgcgaga cattatactt tgcaggttga ggagagtctg cctcacgatg
     1201 taagcgcata tacgcaggga ctcttttttt acgaaggaga gctttatgag tcttgtggtc
     1261 agtatggcga atcatccttc cgcaaggtgg agcttgctac cggaaaagtg ttgcaaagaa
     1321 tcaatttcga cagaaaatat ttcctcgagg gagcttgtgc actcaaagat cgcatatata
     1381 ttctcacctg gcaggagaat acctgctttg tatatgatat caaaacgatg tcattaatcg
     1441 gctcggtacg atacagaggc gaggggtggg gtctgaccac tgatggtaaa tacctgataa
     1501 tgagtgacgg cagctcatcg ctcactttcc gcaatcccga taacttcaag gtggaaaaac
     1561 aggtcacggt cacccttaat ggaaagtctc tgcgctatct gaatgaactg gagtatatca
     1621 atggggagat ttgggccaat gtttatggca gcgatgcgat agtgaccatc aacccttcaa
     1681 gcggggcagt tacgggtatg atagattgtc gcaatcttct gccttcagct ttgcgtaagc
     1741 cctccacgga tgtgctaaat ggtattgctt ataatgaccg tgacggagcc atctggctta
     1801 cgggaaaata ttggccgaaa attttccgcg taacgctcgt ggagaaacaa aagtagaaag
     1861 tttccccttt aggggatatt gacatagatt tattaacaat cgggggtgct ccccttgcgg
     1921 gggctgagaa cacacccact aaacctgatc cggataatac cggcgtaggg aggattttta
     1981 tgaaaaaaac attgttatca gcagcatttc tgctgctttg tgcgctctca accgcacagc
     2041 aattggcaca taacatagtg ccggattcag acg
//
LOCUS       DAOSIE010000260         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_46789_length_2064_cov_3.934793, whole genome shotgun sequence.
ACCESSION   DAOSIE010000260 DAOSIE010000000
VERSION     DAOSIE010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46789_length_2064_cov_3.934793"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..552
                     /locus_tag="PLI69_06710"
     CDS             151..552
                     /locus_tag="PLI69_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOG42211.1"
                     /translation="MNKIKSLDDLRKMKQSLKSDISIREKSDKAEEMVRVRVAMATCG
                     IAAGAREVMNTLLRKVEQDSLPVVVTQGGCMGYCYAEPTIEVAVPGKDPVVFSHVDSA
                     RALEIVDKYIVKGELIDGIIPVNYQTIQEKL"
     gene            557..>2064
                     /locus_tag="PLI69_06715"
     CDS             557..>2064
                     /locus_tag="PLI69_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG42212.1"
                     /translation="MVQIKMHLLVCGGTGCRASESDRLVEQLNHHLQLNGLAEEVKVV
                     TTGCFGFCEKGPIVKVIPDNTFYTQVKPEDAEEIIKEHVLKGRKVARLLYEDPETLEH
                     ISDSKHMEFYQKQVRIALRNCGFIDPENIDEYIVRDGYAALGQVLASDPKETIELLKK
                     SGLRGRGGAGFPTGLKWEIAAGNKADQKYVVCNADEGDPGAFMDRSILEGDPNSIIEA
                     MAICGYCIGATKGLIYIRAEYPLAIRRLEIAIRQAREYGLLGKDILGSGFDFDIQLRL
                     GAGAFVCGEETALISSMQGSRGEPTIKPPFPAQSGYMGKPTNVNNVETYANIPPIILK
                     GWEWFNSIGSEKSKGTKVFALAGKINNVGLIEVPMGTTLREVIFEIGGGIKDGKRFKA
                     VQTGGPSGGCLTEQHLDTPIDYEHLTAAGSMMGSGGMIVMDEDDCMVSVAKFYLDFTV
                     EESCGKCAPCRIGNKRMHETLEQICQGKGTLEDLDYLENLGRVIKDTALCGLG"
BASE COUNT          569 a          445 c          537 g          513 t
ORIGIN      
        1 aatctgattt atgaaatggt cagcagcaac atctgaacat ttctttttgt ttatgagaaa
       61 taatatggct atttttggac tttgttttag gaaacccctg tttattctgt cataaaataa
      121 acactttata aataaaaact ctcaaatatt atgaacaaaa ttaaatcgct tgatgatttg
      181 cgcaaaatga agcagagcct caagtcggac atttcaatcc gcgagaaaag tgacaaagcg
      241 gaggagatgg tcagagtgag ggtcgcaatg gcaacttgcg gtattgccgc cggtgcgcgc
      301 gaggtgatga acacgcttct acggaaggta gagcaggatt ctctccctgt cgttgttacc
      361 caaggcggct gtatgggcta ctgctatgct gagcctacca tcgaagttgc cgtaccgggc
      421 aaagaccccg tagttttcag tcatgttgat tcagccaggg cgctggaaat cgttgacaag
      481 tacattgtca aaggtgaact tatagacggt ataattccgg tcaattacca gacaatccag
      541 gaaaagctct aatcgtatgg tacagatcaa aatgcattta ctggtatgcg gcggtacagg
      601 ttgccgtgca tccgagagcg atcgtttggt tgaacagctc aaccatcatt tgcagctcaa
      661 tgggcttgca gaggaggtta aggtggttac caccggctgt ttcggcttct gcgagaaggg
      721 acccattgtc aaagtgattc ccgacaatac attctacacc caggttaagc ccgaagatgc
      781 ggaggagatt ataaaggagc acgttctcaa aggtagaaaa gttgcacgtc ttctctatga
      841 ggaccctgaa acactggaac atatcagtga ctccaaacac atggagttct accagaaaca
      901 ggtgagaata gcactgcgta actgcggatt tatcgatccc gaaaacattg acgagtacat
      961 tgtacgagat ggctacgccg ctctcggtca ggtactcgca tcagacccca aagagactat
     1021 cgaactcttg aaaaagtcag gtttgagagg caggggaggc gcaggtttcc cgacaggtct
     1081 caaatgggag attgcagccg gaaacaaagc cgatcagaag tatgtggttt gtaatgctga
     1141 cgagggtgac ccgggagcat tcatggaccg ttccatactc gaaggagacc ctaattccat
     1201 aatcgaagcc atggctatct gtggttactg tatcggagct acaaagggtt tgatttacat
     1261 cagggctgag tatcctctgg ccatacgtcg tctggaaatt gcaatacgcc aggcccgcga
     1321 atatggcctg cttggcaaag atattctggg tagcggattt gatttcgata ttcaacttcg
     1381 tctcggagca ggagcatttg tttgcggtga ggagacggca ttgatcagct ccatgcaggg
     1441 atctcgcggt gaacccacca tcaagcctcc attcccggca caatccggat atatgggtaa
     1501 gcctaccaac gtaaacaacg ttgaaactta cgccaatatt cctcccatca tattaaaggg
     1561 atgggagtgg tttaattcca taggctccga gaagtccaaa ggtactaagg tgtttgcatt
     1621 ggcaggtaag atcaacaacg tgggtcttat cgaggttccc atgggtacca cccttcgtga
     1681 agtaattttc gagattggag gaggtatcaa agatggtaag cgctttaagg ctgtacagac
     1741 cggaggccct tccggcggat gtctcacaga gcagcatctc gatactccta ttgactatga
     1801 acatcttacc gcagccggct ctatgatggg atccggaggt atgattgtga tggatgagga
     1861 tgactgcatg gtatcggtag ccaagttcta tctggatttt acagtagaag agtcctgcgg
     1921 caaatgcgca ccttgtcgta ttggcaacaa gcgtatgcac gagactttgg agcagatatg
     1981 tcagggcaag ggcacattgg aagatctgga ttacctcgag aatctcggca gggtaatcaa
     2041 agataccgct ctttgcggcc tagg
//
LOCUS       DAOSIE010000261         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_47074_length_2055_cov_3.855000, whole genome shotgun sequence.
ACCESSION   DAOSIE010000261 DAOSIE010000000
VERSION     DAOSIE010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47074_length_2055_cov_3.855000"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1975
                     /gene="metG"
                     /locus_tag="PLI69_06720"
     CDS             <1..1975
                     /gene="metG"
                     /locus_tag="PLI69_06720"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOG42213.1"
                     /translation="YVRYLRMRGRETLFVCGSDEHGVPITIKARKEGCTPQDVVDKYH
                     GIIKKSFADFGIKFDIYSRTSSKIHHKTASEFFRKLYDEGKFVERESEQYYDEEARCF
                     LADRYIVGTCSKCGAEGAYGDQCEKCGSTLSPDELIDPHSAISGAKPVKKLTKHWYLP
                     LDKYEPWLKEWILEGHKEWKSNVYGQCKSWLDGGLQPRAVSRDLDWGVPVPVEGAEGK
                     VLYVWFDAPIGYISNTIELLPDEWEKWWKSKETKLVHFIGKDNIVFHCIVFPSMLKAY
                     GDGYILPDNVPANEFLNLEGEKISTSQNWAVWLHEYLEDFPGQQDVLRYALCANAPET
                     KDNDFTWKEFQTRNNSELVAIFGNFVNRAVVLTHKHFKGAVPANTKPEQIDRETLSQI
                     PDIRKAIEDNLESYKFREALKEAMNLARLGNKYLADTEPWKVAKSDLDRTASILNVSL
                     QICVNLAIAFAPFLPFSVEKLNNILNVAALDSAPTWEDIGKDLLLPVGHKLNPAELLF
                     AKIEDEQVEAQIAKLNATKVANAAAVVEKAAQEEPVIAPAKAECTFDEFQAMDIRTAT
                     VLEAERVPKTDKLLKLTIDTGLDIRTIVSGIAEYYTPEELVGKQICILANLQPRKIRG
                     IESKGMILMAKQEDGKMRIVSPEEKLLNGSCIS"
BASE COUNT          589 a          444 c          501 g          521 t
ORIGIN      
        1 atatgtgagg taccttcgta tgcgcgggag agagactctc tttgtatgcg gttcggatga
       61 acacggagtt cccattacaa ttaaggcgag aaaggagggt tgtaccccac aggatgtggt
      121 tgacaaatac catggaataa taaaaaagtc ctttgccgat tttgggataa agtttgacat
      181 ctattcccgt accagttcga agattcacca taaaactgct tccgagtttt ttaggaaact
      241 ctatgacgag ggtaagtttg tggagaggga gagcgaacaa tattatgatg aggaggccag
      301 gtgttttctt gcggacagat atatagtagg tacctgctca aaatgcggtg cagagggagc
      361 ttacggcgat caatgtgaga agtgtggaag tactctcagt cctgatgaac tgattgatcc
      421 tcattcggcc ataagcggtg ccaaacctgt aaaaaagttg actaagcact ggtatctccc
      481 gctggacaaa tatgagccct ggctcaagga gtggatttta gagggtcaca aagagtggaa
      541 gagcaacgtt tacgggcagt gtaaatcctg gctggatggc ggtcttcagc cgcgtgccgt
      601 gagtcgtgac ctcgattggg gagttccagt tcccgtggag ggcgccgagg gtaaagtgct
      661 ttatgtttgg tttgatgccc ctatcggata tatttccaac accatagaac ttcttcccga
      721 tgagtgggaa aaatggtgga agagtaagga aaccaaactt gtgcatttta taggaaaaga
      781 taatatagta ttccattgta ttgttttccc ttcaatgctt aaggcgtatg gggacggtta
      841 tatccttccg gataatgttc ccgccaatga gttcctcaat cttgaaggtg aaaagatttc
      901 cacctctcaa aactgggctg tatggcttca tgaatatctt gaggatttcc ccggacagca
      961 ggatgtatta cgttatgcgc tttgtgccaa tgctccggag accaaggata acgattttac
     1021 ctggaaagaa tttcagacac gcaacaatag cgaattggtg gccattttcg gcaatttcgt
     1081 caaccgtgca gttgtactta cacataaaca tttcaaaggg gctgttcctg caaatacaaa
     1141 accggagcag atagacaggg aaacactttc ccagattccg gatatccgta aggcaattga
     1201 ggataatctt gagagttata aattccgcga agcacttaag gaggcaatga atctggctcg
     1261 tcttggaaac aaatatcttg cagatacgga accatggaaa gtggccaaga gtgatctgga
     1321 tcgtaccgca tcaattctaa atgtgtcgct tcagatctgt gtaaatcttg cgattgcctt
     1381 cgcacctttc cttcctttct cggttgaaaa gcttaacaat atactcaatg tggcagcact
     1441 tgactcagca ccgacatggg aagatattgg aaaagatctg cttctacccg taggtcataa
     1501 actcaatcct gcggagttgc tatttgccaa gatagaggat gagcaggttg aggcccagat
     1561 agctaaacta aatgctacca aggttgcaaa tgcagcagct gttgtagaaa aagcagctca
     1621 ggaagagccc gtgattgctc cagccaaggc agaatgtact tttgatgagt ttcaggctat
     1681 ggatatccgc actgctaccg tactcgaggc tgaaagagtg cccaaaaccg acaaacttct
     1741 caaacttacc atagacacag gccttgatat ccgcaccata gtatcaggta tcgcggaata
     1801 ctacactccc gaagagttgg tcggcaaaca aatctgtatc ctcgccaact tacagccacg
     1861 caagattcgg ggcatcgagt caaagggaat gatccttatg gctaagcagg aagatggcaa
     1921 aatgcgtatc gtctctccag aagagaaact gctcaatggc tcttgcataa gctaactcgg
     1981 aactcaggac ttccactcct cataaaactc gcaccccgca ccctgaaact cgcacctcag
     2041 acacgatatt tctcg
//
LOCUS       DAOSIE010000262         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_47113_length_2054_cov_3.642321, whole genome shotgun sequence.
ACCESSION   DAOSIE010000262 DAOSIE010000000
VERSION     DAOSIE010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47113_length_2054_cov_3.642321"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLI69_06725"
     CDS             complement(<1..360)
                     /locus_tag="PLI69_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOG42214.1"
                     /translation="MEEIVKNEAPERNLNFIEEIIEADLASGKHKSIITRFPPEPNGY
                     LHIGHAKSICLNFGLALKYGGRTNLRFDDTNPVKEDVEYVDSIKEDIKWLGFEWANEF
                     YASDYFEQLYQWAILLIK"
     gene            455..>1183
                     /locus_tag="PLI69_06730"
     CDS             455..>1183
                     /locus_tag="PLI69_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HOG42215.1"
                     /translation="MTRSMTGYGKAECLILEDKYLIEIRSLNGKNADITLKTQLIPRE
                     KEISVRNYLIQRLNRGNIDLFASVEQSGENAARYINGQILLGYYNQLCLILDDSPMAD
                     LDREDLLPALLKMPDVVESKATELTEEDWEELFKAICEAADALDNFRLREGEVLREDV
                     LSRVSIIEECLDEVEQFEQERIETIRQRLLSRIEEISLQPDQERLEQEMVFYIEKLDI
                     NEEKVRLRQHCSYFKGTLDNEEYPG"
     assembly_gap    1184..1193
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1252..1371
                     /locus_tag="PLI69_06735"
     CDS             1252..1371
                     /locus_tag="PLI69_06735"
                     /inference="COORDINATES: protein motif:HMM:NF019940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HOG42216.1"
                     /translation="MGREINTIGSKANHAGIQKIVVKMKDELEKIKEQSLNIL"
     gene            1394..2054
                     /locus_tag="PLI69_06740"
                     /pseudo
     CDS             1394..2054
                     /locus_tag="PLI69_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640524.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
BASE COUNT          579 a          416 c          507 g          542 t
ORIGIN      
        1 tttgatcagg aggattgccc attgatagag ttgttcgaaa taatcggaag cataaaattc
       61 gtttgcccat tcaaacccaa gccatttgat atcctccttt atggaatcaa catactccac
      121 atcctccttt acagggttgg tatcgtcaaa acggaggttt gtgcgtccac catattttag
      181 agccagtccg aagttgagac agatgctctt ggcatggcca atatgaaggt atccgttggg
      241 ttcaggcggg aagcgggtaa taattgattt atgtttacca ctggccaggt cagcttcgat
      301 gatctcttca ataaaattca ggttgcgctc gggcgcctca tttttaacaa tttcttccat
      361 agtgccaata aatataggga cggttgtcct gttaaccgac ttgcaaaaat aagaaaaaaa
      421 cgcttacttt ggtatatttt acaccacttg tagtatgaca cgttcaatga caggttacgg
      481 caaggcggaa tgcctgatct tagaagacaa atatttgatc gaaatccgct ctcttaatgg
      541 caagaatgcc gacattaccc tgaaaacgca gttgatacct cgcgagaagg agatttcggt
      601 gcgtaattat ctcatacagc gtctcaaccg tggcaacata gatctgtttg ccagtgtgga
      661 gcaaagtggg gagaatgcag cccggtatat aaacggccaa atacttttgg gttattacaa
      721 tcaattatgt ttgatacttg acgatagccc tatggcagat cttgataggg aggatttgct
      781 gccggcacta ctaaagatgc cggatgtagt ggagtccaaa gccacggaac ttacggaaga
      841 ggattgggaa gagcttttca aggcaatatg tgaggctgcc gatgctcttg ataattttcg
      901 ccttagggaa ggggaggtat tgcgcgaaga tgtactctca agagtctcca ttattgaaga
      961 gtgtttggat gaggtcgaac agtttgaaca ggagaggata gagactataa ggcagcgcct
     1021 cctcagtcgt attgaggaaa tatcgctgca acctgatcag gagcgcctgg agcaggagat
     1081 ggtcttttat attgaaaaac tcgatatcaa tgaggaaaag gtgcgccttc gccagcattg
     1141 cagttatttc aagggaactc tcgacaatga ggaataccct gggnnnnnnn nnnagctact
     1201 ctaaaggatg aggagtatcc cgggcgcaaa ctgggattca tcgctcagga gatgggacgc
     1261 gagatcaata ccataggctc aaaggccaat catgccggga tacagaagat tgtggtgaag
     1321 atgaaagatg aattggaaaa gatcaaagaa cagtcgctta atattcttta aactatttta
     1381 aatatcatat tttatgaaaa aacaactgtt gtcattatta attctggcgt ttccgctttt
     1441 attatccgcg tcagaaaatg aggcccgtct gctgcgtttt ccaacaatag gaggcggtaa
     1501 gatcgtattc agttatgccg gtgaccttta cagcgtggat gctgatggag gcgctgctac
     1561 cagactcacc tcacatgtcg ggtatgaggt atttcccaga atttctcccg atggcaaaac
     1621 aattgtattc acgggccagt acgatggcaa taccgaggtt tattcaatgc ccatagaggg
     1681 tggagagccg gtcagactta cctggacacc tactctggat cgtgatgata tgggcgaccg
     1741 tatgggtccc aacaacatag tgatgacctg gacacccgat ggtaagcaga ttgttttccg
     1801 taccagatgg tatacatttt ccggccttag agggcgtctt tacaaggtct ccgttgccgg
     1861 tggaatacct gaacagatac ctaccacgga gggtggattc tgctctttct cgcctgacgg
     1921 caaatatatg gcgatgaaca ggatgttccg ctaattccgt acctggaaat attataaagg
     1981 tggtcaggcg gatgatatat ggatccataa agtaggtacc acctccctgg agaaaataac
     2041 cgataatcct aatc
//
LOCUS       DAOSIE010000263         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_47343_length_2047_cov_4.709337, whole genome shotgun sequence.
ACCESSION   DAOSIE010000263 DAOSIE010000000
VERSION     DAOSIE010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47343_length_2047_cov_4.709337"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLI69_06745"
     CDS             complement(<1..531)
                     /locus_tag="PLI69_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOG42217.1"
                     /translation="MVKVKPFAAIRPPKAIVKEVAARPYDVLNSVEAKAEAGEKSLLH
                     ITKPEIDFDPIIDEHSQPAYDKAVENFKLWMKKGWLVQDATENYYVYAQTMDGRTQYG
                     LVVCANVEDYLTGKIKKHELTRKDKEEDRMIHVRIQNANVEPVFFTYPDNDEINAIVG
                     RYAAGEPEYDFVADDGF"
     gene            complement(546..1472)
                     /locus_tag="PLI69_06750"
     CDS             complement(546..1472)
                     /locus_tag="PLI69_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG42218.1"
                     /translation="MKVLVATEKPFAKVAVDGIRTIVEEAGHELVLLEKYTSTDQFLK
                     AVADADALIVRSDQVTAEVVAAAPKLKIVVRAGAGYDNLDLAACTAKGIVAMNTPGQN
                     SNAVAELVFGMLIYLQRAGFSGASGTELKGKKLGLHAFGNIGKIVANIAKGFGMEVYA
                     FSPSLARNPKLGEQYGVTAITSPEELYDKSDVVSLHMPANDQTKGSINYDLMSRLPQN
                     GVIINTARKEVINEADLVRIMEERPKFKYGTDIMPGNHEEMVQKFGVRYLASTKKCGA
                     QTEEANVNAGLAAASQICKFFATGDRTFQLNK"
     gene            complement(1478..>2047)
                     /gene="serC"
                     /locus_tag="PLI69_06755"
     CDS             complement(1478..>2047)
                     /gene="serC"
                     /locus_tag="PLI69_06755"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOG42219.1"
                     /translation="SDIMSRPVDVSKYAIIYGGAQKNVGPAGVAFVIIRKDILGKVSR
                     FLPTMLDYRTHINDGSMFNTPPVFPIFVMTETLKWVKSIGGLKAMNKINVEKANLLYN
                     EIDRNSLFVGTAAKEDRSIMNVCFVMAEGYESLQDEFMAFAKERGMVGIKGHRSVGGF
                     RASIYNACPKKSVQALVDCMQEFEKIHKK"
BASE COUNT          525 a          473 c          442 g          607 t
ORIGIN      
        1 gaaaccatcg tccgcaacaa aatcatactc gggttcaccg gcagcatagc ggcccacgat
       61 agcattgatc tcatcattgt cgggataggt gaagaatact ggctctacat tagcattctg
      121 gatgcgaacg tggatcatac gatcctcctc cttatccttg cgggtaagct catgtttctt
      181 tatcttcccg gtcaggtagt cctctacatt ggcacagaca acgaggccgt attgggtacg
      241 tccatccatg gtctgggcat atacatagta gttctcagta gcatcttgta caagccatcc
      301 tttcttcatc cagagtttga agttttcaac tgccttatca taagcgggct gtgaatgctc
      361 gtctatgatg gggtcgaaat caatttccgg ttttgtgata tgtaggagtg acttttctcc
      421 tgcttcggct ttagcctcaa ccgaattaag cacgtcataa gggcgcgctg ccacttcttt
      481 aacaatcgct tttggagggc ggatagccgc aaaaggtttt acttttacca tattatcaga
      541 tgaaattatt tgttgagttg gaatgttctg tcgcctgtgg cgaagaattt acaaatttga
      601 ctggcggcgg cgaggccggc gtttacatta gcttcttcgg tctgagctcc acatttttta
      661 gtagatgcca gataacgaac accaaacttt tgaaccattt cctcatggtt gccgggcata
      721 atgtcggttc cgtacttaaa cttaggacgt tcctccataa tacgcaccaa atcagcttca
      781 tttatcacct cttttcgtgc agtattgata atgacgccat tttggggtaa acgactcatt
      841 aaatcataat tgatacttcc tttagtttgg tcgttagcag gcatatgcag agaaactaca
      901 tcgctcttgt catataactc ttcaggtgaa gttatggctg taacaccata ttgctctccc
      961 agtttaggat tacgtgccaa cgaaggcgag aaggcataca cttccattcc aaaacctttg
     1021 gctatgttgg caacaatttt tccaatatta ccgaaggcgt gcaatcccaa ttttttccct
     1081 ttgagttccg tgcctgatgc acctgaaaag cctgcgcgct gcagatagat cagcatacca
     1141 aacacaagtt cggctacagc attggagttt tgaccgggag tgttcattgc aacaatccct
     1201 tttgcagtgc aagctgcaag gtccagatta tcatatccgg ctcctgcgcg tacgacaatt
     1261 ttgagttttg gtgcagcggc aactacttcg gcagtaactt gatcagaacg tacgataagt
     1321 gcgtcagcat ctgcaactgc tttcagaaat tggtcagtag aggtgtattt ctcgaggagt
     1381 accagctcat ggcctgcctc ttcaacaata gtgcgtatgc cgtcaacggc aacttttgca
     1441 aagggtttct cagtagctac tagtactttc atatttctta ttttttatgg attttttcaa
     1501 attcctgcat acaatcaaca agtgcctgaa cggacttttt cggacacgcg ttgtagatag
     1561 aggcgcggaa gccacctaca gagcggtgac ccttgatgcc aaccatacct ctctctttgg
     1621 caaatgccat aaattcatcc tgaagggact catagccttc agccattacg aaacatacgt
     1681 tcatgatgga gcggtcttcc ttggctgcag tacctacaaa gagggaatta cggtcgattt
     1741 cattgtagag aagatttgct ttctcaacat taatcttatt cattgctttt agaccaccga
     1801 tgctctttac ccatttgaga gtctccgtca ttacaaaaat ggggaatacg ggaggggtat
     1861 tgaacattga accgtcattg atatgggtcc tatagtcaag cattgtgggt aaaaatcttg
     1921 aaaccttacc gagaatatcc ttgcgtataa ttacaaaagc aacacctgcg ggaccaacgt
     1981 ttttctgtgc tccgccatat atgatggcat acttggaaac gtcaaccgga cggctcatga
     2041 tatcgga
//
LOCUS       DAOSIE010000264         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_47751_length_2034_cov_2.594240, whole genome shotgun sequence.
ACCESSION   DAOSIE010000264 DAOSIE010000000
VERSION     DAOSIE010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47751_length_2034_cov_2.594240"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1483)
                     /gene="ispG"
                     /locus_tag="PLI69_06760"
     CDS             complement(182..1483)
                     /gene="ispG"
                     /locus_tag="PLI69_06760"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006282708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOG42220.1"
                     /translation="MKKVSFAIGGVQIGGGAPVSVQTMCNTDTRDIETSVRQCREMAA
                     AGADIIRLTTQGLREVEALRQIKAQLRSKGIMTPLVADVHFSANVALAAAEVADKVRI
                     NPGNFAPVHEDACNKFSLLLDKCRKYDTAIRIGLNHGSLGERITHLYGNTPFAMKEAV
                     MEWLRMCIEKDFHKVVISLKASNTRVMTDAYRLLYEEMERMGRIYPLHLGVTEAGNGD
                     EGRIKSAVGISALLSAGIGDTIRVSLTENPVNEIAAGKEIVKSSQMMGNGIQPNSDLP
                     VTAQNWNEWIIRASCKYGPPLLDCKIDEIDLNGATIAGMPVSEEKKEYFRDLLLQATR
                     RKFTRPEYIACPGCGRTQYNLEETFNEVKRRTSHLKGLRIAIMGCIVNGPGEMADADW
                     GYVGEGGGKVTLYRGKTPVMRSVPQEEAIDRLLELIENDKG"
     gene            complement(1507..>2034)
                     /locus_tag="PLI69_06765"
     CDS             complement(1507..>2034)
                     /locus_tag="PLI69_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42221.1"
                     /translation="YSYGFCTVSSTPTVGEDYIQCPKNFSLLYTYYHALWDQLFNFGV
                     QFGVNYGEEGYYSKYEEWGERYEIIEIPLVSQFKIDFSRFRILVNAGGYYGYRLSTAR
                     ERGWDQYDIRHDYGILAGGGFAVVFKPFEIQFEGNYKFSLNSIYHTNKFSDQYWMLCY
                     PRNIMLSVSLFVHLW"
BASE COUNT          528 a          513 c          442 g          551 t
ORIGIN      
        1 gacaaagaag gtatggcgcg gctcaattgc aaagccgaaa tacctacaaa gcctgtttca
       61 ggcgatagag aagcaatatg gctcttctga taatttcttt agagaagccc tcggaatcag
      121 cgacgaattg agggactctt tccgcaatcg catgctggta taattcttga ttaaagggag
      181 atcagccttt gtcgttttcg atgagttcga gaagtctgtc tatggcctct tcctgaggga
      241 cggaacgcat taccggtgtt ttcccacggt aaagagtaac ctttccgcct ccctcaccaa
      301 cataccccca atcagcgtca gccatttcac cgggaccatt gactatacat cccattattg
      361 ctatacgtag tcctttaaga tgggaggtac gtctcttcac ttcgttgaag gtctcctcca
      421 gattgtactg tgtacgacca caaccaggac atgcaatata ttcaggtctg gtaaactttc
      481 ttctggtggc ttgcagcaga agatctctga aatactcttt tttttcctca gaaacgggca
      541 tgccggcaat ggtggctccg tttaggtcta tctcatcaat cttgcagtcg agcagcggag
      601 gaccatattt gcaagaggca cggattatcc attcattcca gttttgtgct gtcacgggaa
      661 gatcactatt cggttgaata ccgttgccca tcatttggga ggattttaca atctctttac
      721 ccgccgctat ctcatttaca ggattctcgg tgagtgatac gcggatggtg tcgcctatgc
      781 ctgcgctcag aagcgcagaa atgcctaccg cagacttgat gcgtccctca tctccgttgc
      841 ccgcttccgt gactcccaaa tggagtgggt aaatccttcc catcctctcc atctcttcgt
      901 agaggaggcg ataggcatct gtcataactc ttgtgttact tgctttaagt gagatgacta
      961 ccttatggaa atccttttca atgcacatcc ggagccactc cataacagcc tctttcatgg
     1021 caaaaggggt attaccgtag aggtgggtaa tcctctctcc gagagagccg tgattgagcc
     1081 ctatcctgat ggcagtatca tatttacggc acttatccag aagtagggag aatttattac
     1141 aggcatcttc gtgaacggga gcaaaattcc cgggattgat tcgtacctta tccgctacct
     1201 ccgcagcagc gagggccaca ttggccgaaa agtggacatc ggctacaaga ggtgtcataa
     1261 tgcccttaga gcgaagttgc gcctttatct gccgcaatgc ttctacctct ctaagacctt
     1321 gagtagtcag gcggatgata tccgcaccag ccgctgccat ctctctacac tgccttacag
     1381 atgtttcaat atcccgtgtg tcggtgttac acatggtctg caccgatacc ggagcccctc
     1441 ctccgatttg cacaccccca attgcaaaag aaactttctt cataacatta tattaaatac
     1501 cccgattcac caaagatgta caaaaagtga aacactaagc ataatgttgc gcggatagca
     1561 gagcatccag tactggtcgc tgaacttgtt tgtatgatag atgctattga gcgaaaattt
     1621 atagttgcct tcaaattgaa tttcaaaggg tttgaagacc acagcaaaac cgccacctgc
     1681 caggatccca tagtcgtgac gaatatcata ctgatcccag cctctctccc ttgctgtgga
     1741 aagacggtag ccgtagtaac cgccagcatt gaccagtatc ctgaacctcg aaaagtctat
     1801 tttgaactgt gaaaccagcg gtatctctat gatctcatat ctttcacccc actcttcata
     1861 tttggagtag tagccctcct cgccataatt tacaccaaac tgcactccaa aattgaaaag
     1921 ctggtcccag agtgcgtgat aataggtata gagcagggaa aagttcttgg gacattgaat
     1981 ataatcttca cctaccgtag gagtggaact tacggtacag aaaccgtaac tata
//
LOCUS       DAOSIE010000265         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_48228_length_2018_cov_4.797759, whole genome shotgun sequence.
ACCESSION   DAOSIE010000265 DAOSIE010000000
VERSION     DAOSIE010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48228_length_2018_cov_4.797759"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1405)
                     /locus_tag="PLI69_06770"
     CDS             complement(50..1405)
                     /locus_tag="PLI69_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOG42222.1"
                     /translation="MRKFFIILMSIFVVYGCGRFSSTDPSINITEDEREARIAIDFSR
                     DSNYIVNYLKLYYPELTAEQVAAWEATNALENRYIEGQKRYFRNAGRNLFRIDKEAKK
                     VFEQVEGVQVDQLHLFLDGYNKEVIKAAREHKKSLVLPVTMTIKYTLTVKPDVVPDST
                     VLRAWMPYPRGDQNSVKDIRLISTSEPEYKISSDRYVHKSIYMEKIAVAGQPTVFSYE
                     LSFKGYNEWYDFNPEDIKPYDTGSRLYKEYTAERATHVIFSDRIKKITDSIVGTETNP
                     YIKVKKIYRWIADNYPWASAREYSTIENIPEYVLDNNHGDCGQVGLLLITMARYAGVP
                     ARWQSGWMLHPNSVNMHDWLEVYYEGIGWVPTDQSFGRGRYNVIDNDDEYHFYTKGLD
                     AFRWIVNSDYSGEFSPAKKFIRSETVDFQRGEVEWEGGNLYFDKWSGWISDIQYKYEE
                     K"
     gene            complement(1449..2015)
                     /gene="efp"
                     /locus_tag="PLI69_06775"
     CDS             complement(1449..2015)
                     /gene="efp"
                     /locus_tag="PLI69_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOG42223.1"
                     /translation="MATTADFKNGLYLYYNGKPCSIVWFQHVKPGKGPAFVKTKLRNL
                     ENGRILENTFTAGERIETITVEHRTYQFLYADDDGFHFMNTETYDQVQLEKDMVENSD
                     LMKDGQTVQMVFLADEERYLTCELPTYVELEVTYTEPAVKGDTASTNAMKAATLETGA
                     EIMVPLFINQGDRIRVNTTDRSYGERVR"
BASE COUNT          541 a          481 c          419 g          577 t
ORIGIN      
        1 ggaattattt atgaaacaaa aacattaacg tccggatagt attcgaatct tacttctcct
       61 cgtatttata ctgaatatcg gaaatccagc cactccattt atcaaaatag agattgcctc
      121 cctcccactc cacttcgccg cgctggaaat ctacggtttc actacggata aatttcttgg
      181 caggagaaaa ctcaccggaa tagtcactat tgacaatcca gcggaaagca tccagaccct
      241 ttgtatagaa gtgatactcg tcatcattgt caatgacatt gtaacgtccg cgtccgaatg
      301 actggtcggt aggcacccaa ccgattcctt cgtaataaac ctcgagccaa tcgtgcatat
      361 tgacactatt tggatggagc atccagccgc tctgccatct tgcaggcacc cctgcatagc
      421 gggccattgt aatcagaaga agtccaacct gtccgcaatc accatgattg ttgtcaagca
      481 catactcggg aatgttttca atagtggaat actcacgcgc actcgcccag ggatagttgt
      541 cggcaatcca gcggtagatc ttctttacct taatgtaagg attggtttca gtgcctacta
      601 tagagtcggt tattttctta atgcgatcac tgaagatgac atgggtcgcc ctctccgcag
      661 tatactcctt gtaaagtcgg ctgcctgtgt catagggttt aatatcctcc ggattgaaat
      721 cataccactc attgtatccc ttgaaggaga gttcgtatga aaagaccgta ggctgtccgg
      781 ctacagcgat tttttccata tagatactct tgtggacata cctgtccgaa gagattttat
      841 actccggctc agaagtagaa atcaagcgga tatccttgac gctattctgg tccccgcggg
      901 gatagggcat ccatgcacgt aacaccgtac tgtcgggaac cacatcgggc ttgacagtca
      961 gagtgtactt gatggtcatt gttacgggaa gcaccagact tttcttatgc tcccttgctg
     1021 ccttaattac ctctttgttg tacccgtcga ggaataggtg aagctgatcc acttgtacgc
     1081 cctcaacctg ttcaaatact tttttcgcct ctttgtcgat acggaaaagg ttgcgccccg
     1141 cattgcggaa atagcgcttt tgcccctcta tatacctatt ttcaagggca tttgtagcct
     1201 cccaagcagc aacctgttct gccgtcagct ccggataata gagtttcaga tagttgacaa
     1261 tgtaattgct atcgcgggaa aaatcaatgg cgatcctcgc ctctctttca tcttctgtaa
     1321 tattgatgga aggatcagta gatgaaaaac gtccacatcc gtaaactacg aatatggaca
     1381 ttaagattat aaaaaattta cgcataacgt atattatgta tgtagaaggt taagtcatca
     1441 taaaaatatt accttactct ttcaccgtaa gacctgtcgg tagtgttcac tctgattcgg
     1501 tcaccctggt tgatgaaaag gggaaccata atctctgcac cggtctcaag agtggcagcc
     1561 ttcatagcat tggtggaagc ggtatcaccc ttgaccgcag gttcggtata ggtcacctca
     1621 agttcaacat aggtgggaag ctcacaagtc aggtacctct cctcatctgc caaaaatacc
     1681 atttgtaccg tctgtccatc cttcataagg tcactattct caaccatatc tttctccagt
     1741 tgaacctgat cataagtctc agtattcatg aagtgaaaac catcatcgtc agcatagagg
     1801 aattgataag tgcggtgctc aaccgtgatg gtctcaatcc tttcacctgc ggtaaaggta
     1861 ttctcgagga tacgtccatt ttcaaggtta cggagcttgg ttttgacaaa ggcaggacct
     1921 tttcccggtt ttacatgctg aaaccataca atagaacagg gtttgccatt atagtataaa
     1981 tagagtccgt ttttaaaatc tgcagttgtt gccataaa
//
LOCUS       DAOSIE010000266         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_48260_length_2017_cov_2.933231, whole genome shotgun sequence.
ACCESSION   DAOSIE010000266 DAOSIE010000000
VERSION     DAOSIE010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48260_length_2017_cov_2.933231"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PLI69_06780"
     CDS             complement(<1..855)
                     /locus_tag="PLI69_06780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG42224.1"
                     /translation="MAKFLYNKEELRFDKKKFSIWRALLVVGKYVLFSLLVAVIYYAI
                     YSIFFSTDREKQLIAERDYMEREYDRMKEQIEIVESVVEGLEARDRQIYNDIFQSAPP
                     TYMVGRDTGRMDMGTIYEEDENDLIWNVNIRASRLESISGRINTSIENIYHRLSAGET
                     DWQSIPSIIPVKDFSIARTGASVGNKFSPFYKTLRLHTGFDILSPVGTEVLATASGTV
                     TFVTRGTTAWGRRIEITHSGGIVTTYSHLQEIMVKKGQKVQQSDIIGRVGNSGTSFAP
                     HLHYEVIVD"
     gene            complement(859..1650)
                     /locus_tag="PLI69_06785"
     CDS             complement(859..1650)
                     /locus_tag="PLI69_06785"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG42225.1"
                     /translation="MILESRNQELLSQIKSLNQKLDEGHRILNEIEMRDNIVYRPIFG
                     MEEISSEVRDAGFGGVDRYSHLKHFEHSDLLISSAMRLDILSKKAAVQSRSFDEVSLL
                     AKRAGDMASCVPSIFPVSTHPRNSLGSRFGYRSDPFTKGIRMHKGVDISGKTGEPIYA
                     TGDGIVVEVSYNFFGYGNQVIVDHGFGYKTRYAHLHQATVKPGHRVTRGEQVGKMGTT
                     GRSKGVHLHYEVIYQGKAVNPCNYFDHEISPEEYQSMILAGKDRK"
BASE COUNT          554 a          471 c          424 g          568 t
ORIGIN      
        1 gtcgactatc acttcatagt gaagatgggg agcaaatgac gtgccgctat tgccgactct
       61 gcctataatg tccgactgtt gcactttctg gccctttttg accatgatct cctgaaggtg
      121 cgaataggtt gtgactatgc cccctgagtg ggttatctct atacgcctgc cccaggctgt
      181 ggtccctcta gtgacaaatg tgacagtgcc cgatgcagta gcaagcactt cagtgcccac
      241 cggggagagt atgtcaaaac cggtatgcag gcgaagcgtc ttgtaaaaag ggctgaactt
      301 attacccacc gaagctccgg ttctggctat ggagaaatcc tttacgggaa tgattgaagg
      361 aattgattgc caatcagtct ctccggcaga taagcggtga tagatatttt ctatggaagt
      421 attgattctt ccggagatcg attcaagacg ggatgcacgg atgttgacat tccagatgag
      481 gtcattttca tcctcttcat aaatggtgcc catatccatg cgaccggtat cgcgaccgac
      541 catataggtc gggggagcag actggaaaat gtcgttgtat atctgtcgat cccttgcttc
      601 aagcccctct acaacgctct ccacaatctc tatctgttcc ttcatcctat cgtattcacg
      661 ctccatataa tccctttcgg caatcaattg cttttcacgg tcggtgctaa agaatatgga
      721 atagatagca taatagatta cggcgacaag caaactgaaa agtacatact tgcctacaac
      781 aagaagagcc ctccagatgg agaacttctt tttgtcaaat ctaagttcct ctttgttgta
      841 taaaaatttg gccatatctt acttgcggtc tttacccgca agaatcatgc tctgatactc
      901 ttcaggcgag atttcatggt caaaatagtt gcaaggattg acagccttgc cctgatagat
      961 tacctcataa tggagatgga cccccttgga acgtcctgta gttcccattt tacccacctg
     1021 ttccccacga gtaactctat ggcccggttt taccgtagcc tgatgaaggt gtgcatagcg
     1081 tgtcttgtaa ccaaatccat gatccacgat cacctggttg ccataaccga agaaattgta
     1141 gctcacttcg actacgatac cgtcaccggt agcataaatg ggttcgccgg tcttgcctga
     1201 aatatccacc cctttatgca ttctgatacc tttggtaaag ggatctgagc gatatccgaa
     1261 cctgcttccc aggctgttgc gaggatgagt agagacggga aaaatagaag gcacgcatga
     1321 agccatgtca cctgcccttt tggctaacag ggaaacctcg tcaaatgaac gggactgtac
     1381 ggctgccttc tttgagagta tatcgagtct catcgcggat gagattagga ggtctgaatg
     1441 ttcaaaatgt ttcaggtgcg aatatctatc cacgccaccg aagcccgcat cgcgtacctc
     1501 cgatgaaatt tcttccatac cgaaaatagg acgatagact atgttgtccc tcatctctat
     1561 ttcattaagg atacggtgac cttcatcgag cttctgattg agacttttga tttgtgagag
     1621 caattcctgg ttgcgactct caagtatcat ggttttgggt gtcttcagtg agagaacatc
     1681 cgaataaagc caaaagtaag cagcgaagca gataaaacca agagctaaaa ccacactaaa
     1741 ccttaataat ctgctcttta gggtagtttt tactacttcg tatgaaaggg tttccttgtt
     1801 aaaaatgtat tgttttttac ctaacataat caaataacac catttataac cctgcaaaat
     1861 tacacaattt ggataaaaaa actaattttg cgatgaaaaa tcatatttta ttaacaaagt
     1921 tattaacaat ctaactacat gacatctaac gaaacacgtc gtaccttcct cgattttttc
     1981 gcttcaaaag gccataccgt ggtaccttca gcaccta
//
LOCUS       DAOSIE010000267         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_48919_length_1997_cov_3.335736, whole genome shotgun sequence.
ACCESSION   DAOSIE010000267 DAOSIE010000000
VERSION     DAOSIE010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48919_length_1997_cov_3.335736"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLI69_06790"
     CDS             complement(<1..197)
                     /locus_tag="PLI69_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOG42226.1"
                     /translation="MKPTLLVLAAGMGSRYGSLKQMDGLGPNGETIIDYSVYDAIRAG
                     FGKVVFVIRHSFAEEFREIFSP"
     gene            complement(214..>1997)
                     /locus_tag="PLI69_06795"
     CDS             complement(214..>1997)
                     /locus_tag="PLI69_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG42227.1"
                     /translation="CWWVGMKTALQLLIKGDNIRGSTLILAPEDAKRGVSVEKIYNGD
                     SPNYIFADIKIGARTPAAGYHFILITPDKDSIRFEYTLNSREKGSSQRASFGPQDVVY
                     LLMPDRFANGDYSNDSVEEAIEKCNRLLPEGRHGGDIRGIINHLDYISDLGVTAIWST
                     PMLFDNEKKYSYHGYACADYYRIDPRYGTNDLYRDFVEKASEKGIKVIMDMVPNHCGL
                     AHWWMEDLPFDDWVHIYDSFTPSSFALSTHYDPYASKYDKDACIKGWFDTTMPDMNLS
                     HPFVLKYFTQMAVWWVEYAGLGGIRVDTFPYSDRAAMSKWVKNILREYPKMNIVGECW
                     YGDPLSCSYWEGIKQHDGYNSNLPSIMDFPLRDAIIAGFADDSDTPFWGEGIIKVYNS
                     LSLDFVYDNPSNILIFANNHDTNRLAHDLKGDFAKQKMVMALLATMRGVPQLYYGDEL
                     GFVSKDGTTGHSQERIDFPGGWKEDRINLFTRNGRSAAQRDLFYYTSRLMNWRKGSKA
                     VTQGSMIHFRPEHDNVYVYFRYVRKEVVMVVINNGKSDFRVDWERYKEISSKFAASIR
                     NVITGETFRLNSDITVPGQSAAIFEFK"
BASE COUNT          570 a          494 c          398 g          535 t
ORIGIN      
        1 gggctaaaaa tctccctgaa ttcttcggcg aaagagtgcc tgattacaaa cacgaccttg
       61 ccaaaacctg cccggattgc atcataaact gaataatcta taattgtctc gccattggga
      121 ccgagtccgt ccatctgttt aagggaacca taccttgaac ccataccggc cgcgagtacc
      181 aaaagtgtcg gtttcatact ataatacatc tgtctattta aactcaaata tcgctgcgct
      241 ctgtcccggc accgtaatgt cactattcaa acgaaaagtc tctccggtga tgacattgcg
      301 aattgaagcc gcaaatttag aagatatttc tttatatctt tcccaatcaa ccctaaaatc
      361 gctctttccg ttgttaatta caaccataac aacctctttt ctcacatatc tgaaatagac
      421 gtagacattg tcgtgttcag ggcgaaagtg gatcatagat ccttgtgtga cagcttttga
      481 acccttcctc caattcatga ggcgggaagt gtagtaaaac aaatctctct gagcagcact
      541 ccttccattc ctggtaaaaa gattgattcg gtcctccttc cacccaccgg ggaaatcaat
      601 cctctcctga gagtggccgg tggtcccatc cttactcaca aaccccaact catctccata
      661 atagagctgc ggcactccgc gcatcgtagc cagtagcgcc atcaccatct tttgctttgc
      721 aaagtccccc ttgagatcgt gcgccagacg gttagtatcg tggttgttgg caaatatgag
      781 gatattggaa ggattgtcat atacaaaatc cagcgaaagt gagttataaa ccttaataat
      841 cccttcaccc caaaacggag tgtcggaatc atccgcaaat cccgcaatga tggcatctct
      901 taatggaaag tccattatgg aagggaggtt tgaattgtat ccatcatgct gttttatccc
      961 ctcccaataa gaacatgaaa gcggatctcc ataccaacac tcacccacaa tattcatttt
     1021 cggatattcc ctaagtatat tcttaaccca tttggacatc gcagcccgat cagagtaagg
     1081 gaaggtatcc acacgtatgc cgcccaggcc tgcatattct acccaccata ctgccatctg
     1141 agtaaaatat ttaagcacga aagggtgcga tagattcata tcgggcatag tggtatcaaa
     1201 ccaacccttt atgcaggcat ccttatcata tttggaggca taaggatcat aatgagtcga
     1261 aagagcaaaa gatgaaggag tgaaagagtc gtagatatgt acccagtcat cgaaagggag
     1321 gtcttccatc caccagtgtg ccagtccgca atggttaggc accatatcca ttatcacttt
     1381 gatccccttt tccgaagcct tttcaacaaa gtctctgtat aagtcattcg taccgtatcg
     1441 aggatcaatg cgataataat ccgcacaggc atagccatgg taggagtact ttttttcatt
     1501 atcaaatagc atcggagtag accaaatagc cgttactcca aggtctgaaa tataatccag
     1561 atggttgata ataccccgta tatctcctcc atggcgccct tcaggcagta agcgattgca
     1621 tttctcaata gcttcttcta ctgagtcatt tgaatagtcg ccattagcaa aacgatccgg
     1681 cataaggaga taaactacat cttgcggacc aaaggatgcg cgttgcgatg aacctttctc
     1741 gcgactatta agggtgtact caaaccggat ggaatctttg tcgggagtta taaggataaa
     1801 atggtacccc gcagcaggag tcctggcccc aatcttaata tccgcaaaga tatagttggg
     1861 actgtcgccg ttatatattt tttcgaccga tactcctctt ttagcatctt cgggggcaag
     1921 gatcagagta gaaccgcgga tattatcccc tttaataagc aattgaaggg cggttttcat
     1981 ccccacccac cagcatg
//
LOCUS       DAOSIE010000268         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_49375_length_1984_cov_3.799896, whole genome shotgun sequence.
ACCESSION   DAOSIE010000268 DAOSIE010000000
VERSION     DAOSIE010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49375_length_1984_cov_3.799896"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1984)
                     /locus_tag="PLI69_06800"
     CDS             complement(<1..>1984)
                     /locus_tag="PLI69_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOG42228.1"
                     /translation="LYVKVGISSVSVENARANVSSEIKRWHFAEVKYKAEKAWNEYLS
                     KIEVEGDHDLMEVFYTALYHTAIAPNLYSDVNGEYRGMDRKVHKAEGYERYTVFSLWD
                     TFRSLHPLLTIIDRERTVDFIRSFLSIYEEAGKLPIWELAGNETNCMIGYHSVPVIAD
                     ALVKGIEGFDANAALDAMVESSRKAEFGIDIYIDNGLVLAEKEHESVSKTLEYAVDDW
                     CIAQVAKMLGRDDIYKEYMRRAKYYRNIYDPSTGFMRPRINGIWLTPFDPHEVNVHYT
                     EANSWQYTFHVPQDVSGLMDLFGGEKAFEKRLDDLFTAPANISGWNSADMTGLIGQYV
                     QGNEPSHHIAYLYSYAGKPWKTQEIAHKIMTELYTAEPDGLCGNEDCGQMSAWYVFSA
                     LGFYPVMPGSNQYVLGTPLFERAVMHLEDGTDFTLLAPDLSYENFYVSGMKRNGQDYP
                     FAYIRYEDIASGAEFEFEMSEEPDEEFGSDPSARPISAIEGDFVENPWVNVSNPMFLK
                     SVEVSLGALDPDYRIWYSVEPLDREKTDTGIGDFKLYTGPFTLSESSRIRCYCENDSW
                     LRSHITESVVRKISSTYNVTIKEKYSRQYSAGGDLGLVDGIRGGVNFRLGGWQGYQNK
                     DFEAIIDLKESRNVTKLAAGFLQDIKSWIWMPRYVEF"
BASE COUNT          525 a          506 c          420 g          533 t
ORIGIN      
        1 aaactccaca taccggggca tccatatcca ggatttgata tcctgaagaa atcctgcagc
       61 gagctttgtc acatttcggg actctttcag gtcgatgata gcttcaaaat ctttgttctg
      121 atagccttgc caaccgccaa gacggaaatt gacacctccg cggataccat ccaccaatcc
      181 caggtctcct cctgctgaat attgtctgct gtatttctct ttaatggtga cattgtaggt
      241 agaacttatc ttcctgacca ctgattcggt aatatggctg cgaagccagg agtcattctc
      301 acaataacag cggatgcgcg aggattccga cagagtaaag ggtcccgtat agagtttgaa
      361 atcaccgatt ccagtatctg ttttttccct gtcaagaggc tctacactat accaaatccg
      421 gtaatcggga tccaatgcgc cgaggcttac ctcaacactc tttaaaaaca tcggattgga
      481 aacattcacc cagggatttt ccacaaagtc cccttcaatg gccgaaatag gtcttgcaga
      541 tgggtcagag ccaaactctt cgtcaggctc ctcactcatt tcaaactcaa attccgctcc
      601 tgaagcaata tcctcgtaac gaatgtacgc aaaaggataa tcctgaccat ttcttttcat
      661 tccacttacg taaaaattct cataagagag atcgggagcc aaaagggtaa agtcggtacc
      721 atcttccagg tgcatcacgg ccctctcaaa gagaggtgtt cccaacacat attgattact
      781 gcccggcata acaggataga agcccagagc gctgaaaaca taccaggccg acatctgccc
      841 gcaatcctca ttgccgcaaa gtccatcggg ctccgccgta tagagttccg tcattatttt
      901 gtgggctatt tcttgagttt tccagggttt ccctgcataa gagtagagat aagctatgtg
      961 gtggctcggc tcatttccct gaacatactg tccgataaga cctgtcatat ctgccgaatt
     1021 ccatccggaa atatttgcag gggcagtaaa aaggtcatcc aaacgctttt caaatgcctt
     1081 ttctccgccg aaaagatcca tgagtcccga aacatcctgc ggtacatgaa atgtgtattg
     1141 ccaggaattg gcttcagtgt agtgtacgtt gacttcgtgc ggatcgaagg gagtgagcca
     1201 gatgccattg atgcggggac gcataaatcc cgttgaaggg tcgtaaatat tgcggtaata
     1261 ttttgcgcgg cgcatatatt ctttatatat gtcgtcacga ccgagcattt ttgccacctg
     1321 agcgatacac cagtcatcaa cagcatattc gagagttttg gagaccgatt cgtgttcttt
     1381 ttccgccagg acaagaccat tgtcaatgta gatgtcaata ccaaattcag ctttgcgtga
     1441 actctccacc atggcatcca gagccgcatt tgcatcaaat ccctctatcc ctttgaccag
     1501 agcatcggca atcaccggca ccgagtggta tcctatcata cagttggtct catttcccgc
     1561 caattcccat ataggcagct ttccggcttc ttcgtaaatg gagaggaatg agcggatgaa
     1621 atcgacggta cgttccctat cgatgatggt tagcaaaggg tgtagtgacc tgaatgtgtc
     1681 ccagagggaa aataccgtat agcgctcata gccctcagcc ttgtgtacct ttcggtccat
     1741 tccgcgatac tctccgttta catcggaata aagattaggt gcgatagcag tatgatagag
     1801 tgccgtatag aatacctcca tcaaatcatg gtctccttca acttcaatct tggagaggta
     1861 ttcattccaa gccttttctg ccttgtactt gacctcagca aaatgccacc tttttatctc
     1921 ggaagagaca tttgccctcg cattctccac cgaaaccgaa gaaataccca cctttacata
     1981 gagt
//
LOCUS       DAOSIE010000269         1979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_49543_length_1979_cov_3.035343, whole genome shotgun sequence.
ACCESSION   DAOSIE010000269 DAOSIE010000000
VERSION     DAOSIE010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49543_length_1979_cov_3.035343"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PLI69_06805"
     CDS             <1..770
                     /locus_tag="PLI69_06805"
                     /inference="COORDINATES: protein motif:HMM:NF027696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PL29 family lyase N-terminal domain-containing
                     protein"
                     /protein_id="HOG42229.1"
                     /translation="NCWTIKIGETGAENWLMSDGKILTAQPDDLKPAIGIKESSGVYY
                     FTIKYGNGEPQWIVDAEGNPVRASMEIMISPFKSIEILEYSVLITMANDHKISILKAR
                     RGTPSMTISIAPRKNHPDSALMTFDAVNGYYVPGIVENEDYFFEIKVRDTLFNHVNHY
                     LSFVSYLGEANISVTAFAIDNGYIKSIVPDIPKFDVNTVSIGGQQRYYYDSYFGIVYH
                     SGPKDVVKHSRIAVFLKWNGEANTTMKVIELKNKNVP"
     gene            852..1730
                     /locus_tag="PLI69_06810"
     CDS             852..1730
                     /locus_tag="PLI69_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOG42230.1"
                     /translation="MDLKSNKNAGIAEGIVSVIVNTLLFVLKYWAGIVTGSLAIVADA
                     WHTMSDSISSVVLIAATGLSNRKPDKKHPFGHGRWEQIAAIFIGIILAIIAYDFLKNS
                     ILQLKEHTSVTFGTLAIVVTCISIIAKEGLAQYAFHLARKTGNIAIKADGWHHRSDAL
                     SSVVVLIGILLAKYFWWMDSVLGIIIALMLFYATYTIIREAITKILGEEPSDELIKKI
                     TDAVAEMYPEGLQLHHFHIHNYVTHQEMTFHIRLSGELSLEEGHSVATAIEKMIQERF
                     NITSTIHVEPLDFEHP"
     gene            complement(1684..>1979)
                     /locus_tag="PLI69_06815"
     CDS             complement(1684..>1979)
                     /locus_tag="PLI69_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42231.1"
                     /translation="GHNDPILDPIEEYMISARKAAPVTDTLNTFNRWGKIAPEEGGRI
                     IDHIYFRNLTPSLFKTIIGAYAGVEYISDHYPIIAVFALKDAQNQEVRRELSK"
BASE COUNT          579 a          451 c          416 g          533 t
ORIGIN      
        1 ccaattgctg gacaatcaaa atcggagaaa caggggccga aaactggctt atgagcgatg
       61 gcaagatact cacggcacag cctgacgatc ttaaacctgc gattggcata aaggaatcct
      121 caggtgttta ttattttacc atcaaatatg gtaatggtga accgcagtgg atagttgatg
      181 cagaaggtaa tcccgtacgt gccagcatgg agataatgat atcgcctttc aaaagtatag
      241 agatattgga atattccgtg ctgatcacca tggccaacga tcataagatc tcaatcttaa
      301 aagccagacg cggcaccccc tcaatgacta tttcgatagc ccccaggaaa aatcaccccg
      361 actccgctct aatgacattt gatgccgtaa acggctatta tgtgccggga atagtggaaa
      421 atgaggacta tttctttgaa atcaaggtaa gggacactct gttcaatcat gttaaccatt
      481 atttgagctt tgtgtcttat cttggagagg ctaatatatc cgttaccgca ttcgccatcg
      541 acaatggata tatcaagagc atagtacctg acattcctaa gtttgatgta aataccgtct
      601 ccataggtgg acaacagaga tattattatg actcctattt cggtatcgta taccactccg
      661 gccccaaaga tgttgtaaaa cactcaagaa tagccgtatt tctcaaatgg aacggtgaag
      721 ctaacactac tatgaaggtt attgagctta aaaacaaaaa tgtgccttaa tttaaccttc
      781 caacccatag tttgggattt tatacccgga acgtggagct agctccacac atctcgtaac
      841 ctaactacaa gatggatctt aaatctaata aaaatgccgg tattgctgaa ggcattgttt
      901 cagttatagt caacactctg ttgtttgtac tgaaatattg ggccggcatt gtaaccggat
      961 ctctcgctat tgtggcagac gcctggcaca ctatgtccga ctcgatcagt tccgtggtat
     1021 tgattgccgc taccggactc tccaatagaa aacccgataa aaaacatccc tttggccatg
     1081 gtcgctggga acagatagct gccatattta tcggcattat tctcgcaatc atagcctacg
     1141 acttcctcaa gaactccatt ctgcaactaa aagagcacac ctcggttaca ttcggcactc
     1201 ttgctattgt agtgacctgt atctccatta tagccaagga ggggctggcg caatatgctt
     1261 tccatcttgc ccgcaaaaca ggcaatattg ccattaaagc cgacggatgg caccatagga
     1321 gcgatgcact ctcttcagtg gtagtgctaa tcggcatcct gctcgctaaa tacttctggt
     1381 ggatggatag cgtactgggc attatcatag ccctaatgtt attttacgct acttatacca
     1441 taataaggga agctatcacc aagatactgg gagaagaacc ttccgatgag cttataaaaa
     1501 agattaccga tgctgtggca gagatgtatc cggagggact acaactgcat cattttcata
     1561 tccacaacta cgttactcac caggaaatga catttcacat ccggctctca ggagaactct
     1621 cactcgagga aggccactcc gtcgcgactg caattgagaa aatgattcag gagcgcttca
     1681 acattacttc gacaattcac gtcgaacctc ttgattttga gcatccttaa gagcaaaaac
     1741 tgcaattatg ggataatgat cggagatgta ctctactccg gcatatgcac cgataatagt
     1801 tttaaatagt gacggtgtca gatttctgaa atagatatga tcgattattc tcccaccctc
     1861 ttcgggagcg attttacccc aacgattgaa tgtattgaga gtatcggtga caggagctgc
     1921 tttgcgcgcg gagatcatat attcctcaat aggatccagt atgggatcat tgtgccccg
//
LOCUS       DAOSIE010000270         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_50023_length_1965_cov_3.770681, whole genome shotgun sequence.
ACCESSION   DAOSIE010000270 DAOSIE010000000
VERSION     DAOSIE010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50023_length_1965_cov_3.770681"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..731)
                     /locus_tag="PLI69_06820"
     CDS             complement(15..731)
                     /locus_tag="PLI69_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 domain-containing protein"
                     /protein_id="HOG42232.1"
                     /translation="MKELLTKYAIMYNRPEYFETDPVIFPKKFAQLTQSGKASLQDVE
                     IAAVIAAHLAWGRRDITVRNCNRAFDEMEWQPYRYIMEREWRNEKISLHRTIKWSDFA
                     AICFRLRDFYMQYDSLESLTPDLFRTEIYGQKSDPKAANKKIHLLRRWMVRDDGIIDL
                     GLWKNTSPTDLIIPLDVHVHRTASMLGITSRKSTDLSTALEITSFLKEIFPTDPCKGD
                     FALFGYGITHKKEENSNICC"
     gene            complement(809..1060)
                     /locus_tag="PLI69_06825"
     CDS             complement(809..1060)
                     /locus_tag="PLI69_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOG42233.1"
                     /translation="MKKGIHPESYRLVAFKDMSNDHVFITRSCVNTKETLEVDGVEYP
                     LVKVEISNTSHPFYTGKMKLVDTAGRVDKFYSRYKNKKK"
     gene            1196..>1965
                     /locus_tag="PLI69_06830"
     CDS             1196..>1965
                     /locus_tag="PLI69_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009434820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOG42234.1"
                     /translation="MTLIKSISGIRGTIGGAPGDALTPVDIVKFTSAYARQLQRKFPS
                     KKHYKVVVGRDARISGKMVDSIVCGTLMACGIDVVNAGLASTPTTEMAVIFEKADGGI
                     ILTASHNPKQWNALKLLNEKGEFLTAEEGAALLEVAESDDFDYKDVDSIGHMEYKEFT
                     QAHIDAVLSYPLVDLKAIKAAGFKVVLDAINSVGGIVMPRLFEALGVECITINGEPNG
                     HFAHNPEPVPSNLTDLAAAVVAHKADLGVAVDPDVDRLA"
BASE COUNT          500 a          422 c          465 g          578 t
ORIGIN      
        1 catagtacct tttttcaaca acagatattg gaattctctt cctttttgtg tgttatacca
       61 tagccgaaaa gtgcaaaatc acccttgcat gggtccgtgg ggaatatttc cttgaggaaa
      121 gatgtgatct ccaatgctgt tgataaatcg gtacttttac gtgaggtaat acctagcatt
      181 gaggcagtac ggtggacatg gacatcaagc ggaatgataa ggtcggtagg agaggtgttt
      241 ttccacaatc caagatcaat tatgccgtca tcacgcacca tccagcgtct gagcaggtgt
      301 atctttttgt tggctgcctt cggatcgctt ttctgtccgt atatttcagt tctgaaaagg
      361 tcgggagtaa gtgattcgag agagtcgtat tgcatgtaga agtcgcgaag cctgaaacaa
      421 atggctgcaa agtcactcca tttgatcgtt ctgtggagtg atattttttc attgcgccat
      481 tccctttcca tgatatagcg gtatggctgc cactccatct cgtcaaaagc tctgttgcaa
      541 ttccttacag taatatctct tctgccccag gcaagatgtg ccgcaatcac ggcggcgatt
      601 tccacatctt gaagggaagc tttacccgat tgggtcaact gcgcgaattt ttttgggaaa
      661 atcaccgggt ccgtctcgaa atattcggga cggttgtaca taattgcata ttttgtaagg
      721 agttctttca caatcgctgt tgtctgttta aaaaagaaag gtgacctatg aaagtcaccc
      781 ctcaaatata aacaaaacgc cttgcggctt atttcttctt attcttgtat ctgctgtaga
      841 acttgtcaac acggcctgcg gtgtctacga gcttcatctt accggtatag aaagggtgtg
      901 aagtattgga aatctcgacc tttactaaag ggtactcaac gccgtcaacc tcgagagtct
      961 ctttggtgtt gacgcaagag cgtgtgatga acacatggtc atttgacata tccttgaaag
     1021 ctacaagacg gtaggattcg ggatgtatac cttttttcat cttatcggtt tgttaattgt
     1081 taaaatcttt ttcgggctgc aaaagtagaa ataatttggg gaatggccaa atattttgct
     1141 gctttttgta actttgccac atattttcaa gccaaaccta caattttttg atcatatgac
     1201 acttatcaaa tcaatctcgg gcatcagagg aactatcggt ggtgctcccg gcgacgcatt
     1261 aactccggtt gacatagtta aatttacatc ggcctacgct cgtcagctcc aaaggaaatt
     1321 tccatctaaa aagcactata aggtggtcgt aggcagggat gcccgcattt ccgggaaaat
     1381 ggtggattcc atcgtttgcg gtacactgat ggcttgcggt atcgatgtag tgaatgcagg
     1441 tttggcgtcc actcccacaa ccgaaatggc agtaatattt gagaaagccg atggtggtat
     1501 cattctcacc gcttcgcaca atcccaagca gtggaacgcc ctcaaacttc ttaacgagaa
     1561 gggtgaattt ctgacggcgg aagagggcgc ggcccttctt gaagttgccg agtccgatga
     1621 ttttgattat aaagatgtgg attcaatcgg tcatatggag tacaaggagt ttactcaggc
     1681 acatatagat gcagttttat catatcctct cgttgattta aaagccataa aagctgcagg
     1741 ttttaaggtg gttctggatg ccataaactc cgtcggaggc atagtgatgc cgagactttt
     1801 cgaggcgttg ggggtggagt gtatcacaat aaacggtgag cccaacggcc attttgcgca
     1861 caatcccgaa cctgttccct caaatcttac cgatctcgct gcagctgttg tcgcccacaa
     1921 ggcggatctc ggtgttgctg tggatcccga tgtggaccgt cttgc
//
LOCUS       DAOSIE010000271         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_50174_length_1961_cov_3.416055, whole genome shotgun sequence.
ACCESSION   DAOSIE010000271 DAOSIE010000000
VERSION     DAOSIE010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50174_length_1961_cov_3.416055"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /gene="ispF"
                     /locus_tag="PLI69_06835"
     CDS             complement(<1..299)
                     /gene="ispF"
                     /locus_tag="PLI69_06835"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOG42235.1"
                     /translation="MEIRVGNGFDIHELAPGLPLRLCGVTIPAEQGCVAHSDGDVAIH
                     ALCDALLGALALGDIGKYFPDTSDEYKGIDSRILLKRIYEMVKERGWSIGNIDIT"
     gene            380..739
                     /locus_tag="PLI69_06840"
     CDS             380..739
                     /locus_tag="PLI69_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42236.1"
                     /translation="MGFFNLSFFRTPSHRVFHYTPRYYDERKERIAEIYKKYGKNPDG
                     TPFDPSQETTDSKGAKSTREYVPGASIRGSFSKGIEQHRRQAENKKVKKLITLVTFVA
                     LLVALYYIAIGVGQLFK"
     gene            741..>1961
                     /gene="mutL"
                     /locus_tag="PLI69_06845"
     CDS             741..>1961
                     /gene="mutL"
                     /locus_tag="PLI69_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOG42237.1"
                     /translation="MLKLLPANISNLIAAGEVVGRPASVVKELMENSIDAGADTIQLI
                     VTDAGRTLIQVIDNGSGMTPADAELCFERHATSKIDRAEDLESISTYGFRGEALASIA
                     AVAHVTLKTRHSGSDIGWQVEVSESHITASHETSSPIGSGFEVRNLFYNVPARRKFLK
                     SDNSELRHIIQEFIRVAVIRPELKFKMFSNGKVLYDLRATDNTKQRIHDIFGRDMINS
                     LVEIGTETSVVKISGHVGKPESARKTPGNQYFFINGRYFRSPYLHKAVMKAYEQLLPE
                     GMLPTYFVFLETPPDRVDVNIHPTKSEVKFEDEQMIFEIVMASVREALGKNSFVPSID
                     FDTEGVPEIPSMPTQGSMEYGREHIRMPRIDYDPLFNPFDIEKGRDFSTVPSRIRPIG
                     EEQEERMLFEEREIS"
BASE COUNT          566 a          490 c          438 g          467 t
ORIGIN      
        1 atagttatat caatattgcc tatactccaa ccacgctcct tgaccatttc ataaatgcgc
       61 ttaagcagta tgcggctatc gatacctttg tattcatccg aagtatcggg gaaatacttt
      121 ccaatatcgc cgagagcaag ggcacctaac agtgcatcac aaagagcatg gattgccaca
      181 tctccgtcag agtgggcaac acatccctgc tccgcaggaa tagtcacccc acagagcctc
      241 aagggcagcc cgggagcaag ctcatgtata tcaaaaccgt taccgacacg aatctccata
      301 gaaacctcct atttttctac aaaaataaaa aatatgttaa ctttgtccta aatattcaat
      361 tgacattttt gttacaatta tgggattttt caaccttagc tttttcagga caccttctca
      421 ccgggttttc cactacactc cacgctatta tgatgagcgc aaggagcgta tagcggagat
      481 ttataaaaag tatggaaaga acccagacgg cactcccttc gatccatcgc aagagacaac
      541 ggatagtaaa ggtgccaaat caaccagaga gtatgtgccc ggagcgagta ttcggggctc
      601 attcagcaaa ggcattgagc aacaccgccg ccaggctgaa aataaaaaag taaaaaagtt
      661 aattactctg gtaactttcg tagccctgct ggtggctttg tactatatag ccatcggtgt
      721 tggtcaactt tttaaataat atgctaaagc tattacccgc caacatatcc aatctgatag
      781 cagcaggaga agttgttggt cgccccgctt ctgtggtcaa ggagctaatg gaaaactcca
      841 ttgatgcggg agcagacacg attcaactta tagtgaccga tgcgggcagg accctcatac
      901 aggttatcga caatggctcc ggcatgaccc ccgcagatgc tgaactctgc tttgagcgcc
      961 acgccacctc caaaatagac cgtgccgaag atcttgagtc catctccaca tacggtttca
     1021 ggggcgaagc tctcgcctcc attgcggcag tggcacatgt aaccctcaaa acacggcatt
     1081 ccggaagcga tataggatgg caggtagagg tgagcgaatc ccatatcacc gcaagccacg
     1141 aaacctcctc tcccataggc tccggcttcg aggtacgaaa cctcttttac aatgtacccg
     1201 ccagacgaaa atttcttaag tcagacaatt ccgaactacg gcacattatt caggagttca
     1261 taagagtggc cgtcattagg ccggaactca aattcaagat gttctcaaat ggcaaggtac
     1321 tttatgatct ccgtgctacc gacaacacaa aacaacgtat acacgacatc ttcggtcgtg
     1381 acatgatcaa ctctttggta gagattggca ccgaaacatc ggtagtcaag ataagcggcc
     1441 acgtaggcaa gcccgaatca gcccgcaaaa cccccggcaa ccaatatttt ttcattaacg
     1501 gacgctattt ccgctcaccc tacctgcata aggcggtgat gaaggcttat gagcaactcc
     1561 ttcccgaagg gatgttgccc acctattttg tcttccttga gacccctccg gatagagtgg
     1621 atgtcaatat tcatcccact aagagcgaag taaagttcga ggatgaacag atgatatttg
     1681 agatagtgat ggcttctgtc agagaggcgc tcggcaagaa ctcttttgta ccgagcatag
     1741 attttgacac ggaaggggtt ccggaaatcc ccagtatgcc tactcagggc tcgatggagt
     1801 acggtaggga acatattaga atgcccagga tagactatga ccccttattc aatcccttcg
     1861 acatagaaaa gggcagggat ttttcgaccg taccttctag aattcgtccc attggagaag
     1921 aacaggagga gcggatgctc tttgaggaga gggagatctc g
//
LOCUS       DAOSIE010000272         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_50346_length_1956_cov_3.290900, whole genome shotgun sequence.
ACCESSION   DAOSIE010000272 DAOSIE010000000
VERSION     DAOSIE010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50346_length_1956_cov_3.290900"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLI69_06850"
     CDS             complement(<1..696)
                     /locus_tag="PLI69_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HOG42238.1"
                     /translation="MKLTFSIGGIHPKENKLSRDAHIETFPVLDVAYISMAQHLGSPA
                     EPVVQKGDKVKVGQVIGKPTGFISGFVHSSVSGTVTAVEPKGDLAGNPVMHVVIAVEG
                     DEWCEEIDRSKDIVREIPFSSEEILEKIKNAGVVGLGGAAFPTHIKLNPPKDKKAEWL
                     IINGTECEPYITSDDRIMRERPEEIVIGAAIMMKALKVEKCYIGIEDNKPEAITSMTK
                     AASHYPQIKVVSLK"
     gene            complement(709..1590)
                     /locus_tag="PLI69_06855"
     CDS             complement(709..1590)
                     /locus_tag="PLI69_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOG42239.1"
                     /translation="MSTTIIYTIVSLVSLGLLFSVVLYFVAQKFKVEEDPRIDTVESL
                     LPGVNCGGCGLAGCRAFAEEVVKAPSIGSLFCPVGGDPVMEKVAAALGKEAVKTEPKV
                     AVIKCHGTLDNRPKTNHFDGYASCKGMAALYSGDTGCRYGCLGKGDCVNVCLFDAIHI
                     DERRGIVEVDEEKCVACGACVKACPKSIIELRPKGPKNRRVYVSCSSRDKGAIARKVC
                     SVSCIGCGKCVKACPFDAITLENNLAYIDPAKCKLCRKCVDECPTKAIWAVNFPIKVP
                     VAPKQEEGQSSVINNES"
     gene            complement(1587..>1956)
                     /locus_tag="PLI69_06860"
     CDS             complement(1587..>1956)
                     /locus_tag="PLI69_06860"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOG42240.1"
                     /translation="EIINKSACASCHAKGVCISSDESVREIEIPQSISTLASDYKVGE
                     EVKVLLRASLGHRAIWLTAVIPLIILMAIILLFTNIGISELVTGITIIATLALYYFFV
                     YLFKNKISKYFTFSIEKLSK"
BASE COUNT          493 a          509 c          439 g          515 t
ORIGIN      
        1 ctttagagag actaccttaa tttggggata gtgagatgcc gcttttgtca tggaggttat
       61 ggcttcgggc ttattatcct ctatgccgat atagcatttc tccactttga gcgccttcat
      121 cataatggca gcgccaatta caatctcttc gggacgctct ctcattatcc tgtcgtcaga
      181 ggttatgtaa ggttcgcact cagtgccgtt gatgatgagc cactcagcct ttttatcctt
      241 gggaggattg agctttatgt gagtggggaa tgcggctccg ccgagtccca caactcccgc
      301 gttctttatc ttttcgagaa tttcttccga agagaatggg atttctctta ctatatcttt
      361 acttctgtca atctcttcac accactcgtc accttctacg gctatcacaa cgtgcatcac
      421 tggattgccc gccagatcac ccttgggctc tactgcggtc accgtgcctg acacggatga
      481 gtgtacgaag cctgaaataa agcctgtcgg cttaccgata acctgaccca ccttgacctt
      541 atctcctttc tgtactaccg gctcagccgg agaacccagg tgctgggcca tcgagatata
      601 agccacgtca agtaccggga atgtttctat gtgtgcgtct cgagagagtt tattctcttt
      661 agggtggata ccacctatgg aaaatgtaag tttcatatct gttcctccct aactttcatt
      721 gtttataacg ctgctctgcc cctcttcttg cttaggtgca acaggcacct taatgggaaa
      781 attgaccgcc catatagcct tagtgggaca ttcatccaca cacttgcgac acagcttaca
      841 tttagccgga tctatgtagg cgaggttgtt ttcaagggta atggcatcaa aaggacaggc
      901 cttgacgcat ttaccacagc cgatgcagct aacggagcag accttgcggg caattgcacc
      961 cttgtcccgg ctcgaacagg agacatacac cctgcgattt ttagggcctt tcgggcgaag
     1021 ttcgataata gacttgggac aggctttaac acaagccccg caagctacgc acttctcctc
     1081 atcgacctca acgatgccac gcctttcatc gatatggatg gcatcaaaca ggcagacatt
     1141 gacacaatcc cccttgccca gacagccata cctgcaaccg gtatctccgg agtatagggc
     1201 ggccatcccc ttgcaggagg catatccgtc aaaatgatta gtcttcggtc ggttgtccag
     1261 agtgccgtga cacttgatga cggccacctt cggctcggtt ttgactgctt ccttaccgag
     1321 agccgcagca actttttcca taacgggatc accgccaacc ggacagaaca atgaacctat
     1381 ggatggggcc ttcacaactt cttcagcgaa tgcacggcaa ccggccaggc cgcatccgcc
     1441 gcaattgact ccgggaagta gtgactctac cgtgtctatc ctcggatcct cttcaacctt
     1501 gaacttttgc gccacaaagt agagaacaac cgaaaaaagc agcccaagcg ataccaaaga
     1561 aacaatcgtg tagataatcg ttgtactcat ttggatagtt tttcaataga aaaggtaaaa
     1621 tatttggata ttttgttttt gaacagataa acgaaaaaat aatagagggc aagggtggcg
     1681 ataatagtga tgccggtaac gagctcgctg atgccaatat ttgtaaatag aagaataatg
     1741 gccataagta taatcaaggg gataacggca gtcaaccaaa tagctctatg gccaagggat
     1801 gccctcagga gtaccttcac ctcttctccc accttataat cactcgccag agtcgaaatg
     1861 gactggggaa tctctatttc tctgaccgac tcatccgatg agatgcagac accttttgca
     1921 tggcaggatg cacaagccga tttgttgatt atctcc
//
LOCUS       DAOSIE010000273         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_50517_length_1951_cov_3.407173, whole genome shotgun sequence.
ACCESSION   DAOSIE010000273 DAOSIE010000000
VERSION     DAOSIE010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50517_length_1951_cov_3.407173"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLI69_06865"
     CDS             complement(<1..386)
                     /locus_tag="PLI69_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOG42241.1"
                     /translation="MKVKFLLVGKSVESWLQSGIKLYTSRLKHYVPFEMTVIPELKQV
                     SALSVEQIKEREGDLILARLKSSDDVILLDERGNMYSSEDWARHLEQKMTYGSKDIVF
                     VIGGAYGFSEKVYQRADGKLSLSKMT"
     gene            493..927
                     /locus_tag="PLI69_06870"
     CDS             493..927
                     /locus_tag="PLI69_06870"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOG42242.1"
                     /translation="MKKILLLAMFGIFSIAAPAQMMQLVNSSQTNVQSNTEQDVFTPI
                     GKYIQSGDYDKLSAWFADNLELNILGAINNCTRNQARLIIKNFFTNYTPKKFTIIHKS
                     GKAPMKYAVGSLSAGGEKFRVILYVKTTDGVNSIQQLKVERE"
     gene            complement(908..1762)
                     /locus_tag="PLI69_06875"
     CDS             complement(908..1762)
                     /locus_tag="PLI69_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOG42243.1"
                     /translation="MRLNNIKFAFLYVALLAMVASCSPRHLVILHTNDTHSQIEPVRV
                     GRDRDKGGVERRLQYINSVREEAGRRNLLLLDAGDYNQGTPYFTMAKGDLEIELMNAL
                     GYDVATLGNHEFDNGQEELARRLSASEFVTVTCNYDFSDSVLKDYVKPYTIIRRAGLK
                     IGIVGATSYLEGVVLKSHLEGLVRLDAISEVNKWAAWLKHKKKCDLVILLSHFGHQGG
                     SMEHPSDILMAQNSKDIDIIIGGHSHTFLKNAVEVENLEGRKVVITQCGGQGVIVGRM
                     DIPTPVRP"
     gene            complement(1759..>1951)
                     /locus_tag="PLI69_06880"
     CDS             complement(1759..>1951)
                     /locus_tag="PLI69_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42244.1"
                     /translation="NFLMDGGDRLSVGDFAKNITNLENVFIRDAIVDYIREMTARGET
                     ISLKNDGRVIVKNMEDSGR"
BASE COUNT          548 a          478 c          358 g          567 t
ORIGIN      
        1 aatgtcatct ttgagaggga aagttttcca tcggcacgct gatatacttt ttccgaaaat
       61 ccgtatgcac ctccgatcac aaagactata tctttggatc cgtatgtcat tttttgctcc
      121 aaatgtcgtg cccaatcttc tgaggagtac atattgcccc gctcgtcaag caagatcacg
      181 tcatccgaac tctttaatct ggcaagaatg aggtcccctt ccctctcttt gatttgctca
      241 acagaaagag ccgagacctg tttaagctcg ggaataaccg tcatttcaaa tggcacatag
      301 tgcttgagac ggctggtata gagcttgatg cctgactgca gccagctctc cacactctta
      361 cctacaagga gaaatttgac tttcataata caaaaataac atttttaagg ggattcgtcc
      421 cttcaatggc ttgatttttg tttgtttgta tttgataagc agttttatta tttcttaaac
      481 ctataacgcc aaatgaagaa gattttactc ttggccatgt tcgggatatt ctccattgct
      541 gcaccggctc agatgatgca gttggttaat agttcccaga ccaatgtcca aagcaatacc
      601 gagcaggatg tattcacccc aatcggcaaa tatatccaat ctggcgacta tgacaaactc
      661 tccgcctggt ttgccgacaa cctcgaactg aatattctgg gtgccatcaa caactgtacc
      721 cgcaatcagg cacgtctgat catcaagaac ttttttacca actatacccc taagaaattt
      781 accataatcc acaagagtgg caaggcccct atgaaatatg cggtcggttc actttccgca
      841 ggcggtgaaa aattcagagt aatactttac gttaaaacaa ctgatggggt aaattcaatc
      901 caacaactta aggtcgaacg ggagtaggaa tatccattct acccacgatt acaccttgcc
      961 ctccacattg tgttataacc acctttctgc cctcaagatt ttctacttct actgcatttt
     1021 ttagaaaagt atgagagtgg cctccaatta taatgtctat atcttttgaa ttttgagcca
     1081 tcaaaatatc tgagggatgc tccattgaac caccctgatg accgaaatgt gatagcagta
     1141 tcaccaaatc acactttttc ttatgtttta gccaggcagc ccatttattt acctcggata
     1201 tggcatccaa acgaaccaga ccctccagat ggcttttgag tacaacccct tcgaggtatg
     1261 atgttgcccc tactatacct attttcaatc ctgcacgacg tatgatggta tagggcttga
     1321 cataatcctt caaaaccgaa tccgaaaagt cataattaca ggttacagta acaaattcag
     1381 acgctgatag tcgtcttgca agttcttcct ggccattatc aaactcgtgg tttcccaaag
     1441 tggctacatc gtatccaagt gcattcataa gctctatttc caggtcacct tttgccattg
     1501 taaaataggg tgtgccctga ttatagtcac ctgcatccag caacaataga ttacgacggc
     1561 cggcctcttc tctaacagag ttgatatatt gcagacggcg ctccacgcca cctttatcac
     1621 gatcacgccc cacccttacc ggttcgattt ggctgtgagt atcatttgta tgaagtatta
     1681 caaggtgcct tggcgagcag gatgccacca ttgcaagcaa tgcaacatac aaaaaagcaa
     1741 atttgatgtt attaagtctc atcttccgga gtcctccata ttcttgacaa tcacccgacc
     1801 gtcatttttt aaggaaatgg tttcaccgcg ggcagtcatc tctctgatat agtccacaat
     1861 ggcatcacgg atgaaaacat tttccagatt ggtaatattt ttagcaaaat ctcccacact
     1921 caagcggtca cctccatcca taaggaagtt g
//
LOCUS       DAOSIE010000274         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_50589_length_1949_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAOSIE010000274 DAOSIE010000000
VERSION     DAOSIE010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50589_length_1949_cov_3.000000"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PLI69_06885"
     CDS             complement(<1..716)
                     /locus_tag="PLI69_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOG42245.1"
                     /translation="MIQYKFKTSGKTRLDHDLLGDKEVPVEALFGVQTLRCIENFDIS
                     RDLLGDHPEFIKAFGIVKKGAILANHQLGLVSDEVKDAVVAACEELMEGKHCEHFPID
                     MVQGGAGTSANMNANEVIANRALQIAGHKPGEYQYIHPNDHVNMAQSTNDAYPTAMQL
                     GMYMIHKDVMKAIKALARSFRTKEKKFADVIKMGRTQLQDAVPMTLGQTFGAYASAIE
                     NEMKRLDEAAQGLLVINMGGT"
     gene            complement(783..>1949)
                     /gene="trkA"
                     /locus_tag="PLI69_06890"
     CDS             complement(783..>1949)
                     /gene="trkA"
                     /locus_tag="PLI69_06890"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOG42246.1"
                     /translation="GVENADLFVAVSPAKDQNINIISALLAKKMGAKRVTARINNDEY
                     LDYQNKFFFKELGIDLLFYPEKSAADEIEDLLKKSELSDFVGFANGRLQLVVIKLEEG
                     APLVGQSLADFDYPADNLPFRTVAIARGDETIIPQHETIFKVGDLVYIIALNEAVSEI
                     MSYSGKEEIEIRRITILGGGKIGEILAKRLEPTADFIKIIEINKERCEVLSERLDKTL
                     VINGDGRNSDFLYEEDVKSCDAFIAVTSSSETNILSCVAAKRMGVAKTIAEVENLEYI
                     RLAEEMGVDSVINKKLIAASRIFRVTLSNHIRTIKCLSGSDAEAIEYMVNAGSLITTA
                     PIKELNFPKDAVIGGVIRGQEAFIAVGTTLIKPHDRVVVFALPSALSKLEKFFA"
BASE COUNT          511 a          461 c          402 g          575 t
ORIGIN      
        1 gtaccaccca tattgattac gagcaagccc tgtgctgcct catccagacg tttcatctca
       61 ttttcgatag cggaggcata agcaccgaat gtctgtccaa gagtcatggg tactgcatcc
      121 tggagttgtg tgcgacccat tttgataacg tccgcaaatt ttttctcttt tgtacggaat
      181 gagcgtgcga gagctttgat ggccttcatc acatctttgt ggatcatgta catacccagt
      241 tgcatagccg tagggtatgc atcattagtg gactgggcca tatttacatg atcattggga
      301 tgtatgtact gatactcacc gggcttgtga ccggcaatct ggagagccct gttggcaatc
      361 acctcatttg cgttcatatt ggcagaagta ccggcgccac cctgaaccat atcaatgggg
      421 aagtgttcgc aatgtttgcc ttccattagt tcctcacagg ctgcaaccac tgcatccttt
      481 acctcgtcag aaacaagacc gagttggtga tttgctagaa tagctccttt ctttacaata
      541 ccgaaagctt taataaattc cggatggtca ccgagaaggt cacggctaat gtcgaaattt
      601 tctatgcaac ggagagtctg cactccgaac agcgcctcaa cgggcacttc cttgtcacct
      661 agaaggtcat ggtctaacct tgtcttacct gaagttttga atttgtattg aatcataatg
      721 aagaattagt tgattattat taaattccgc aaactcaata ataattctaa tctcatcaaa
      781 aattaggcga aaaacttttc cagtttactt aaagctgagg ggagcgcaaa gacaacaacc
      841 ctatcgtgag gtttgattaa ggtggtacca acagcaataa atgcttcctg ccctctgatg
      901 actccaccga taacggcatc tttggggaaa ttaagctctt ttatgggagc cgttgtaatc
      961 aaactacctg catttaccat atattcgatg gcttctgcat cggaaccact taaacatttt
     1021 atagtacgga tatgattgga aagggtaact ctgaaaatac gactggcagc gatcagtttt
     1081 ttgtttatta ccgaatccac acccatctct tctgcaagac gtatatattc gaggttttcc
     1141 acttcggcaa tagtcttggc tacccccatc cttttggccg ccacgcagga gagaatattg
     1201 gtttccgaac tggatgttac cgctatgaaa gcgtcacagc tcttgacgtc ctcctcatag
     1261 agaaaatctg aattacgtcc atcaccgttg attacaagag ttttatccaa acgctcagaa
     1321 agaacttcgc aacgttcttt attgatctca attatcttta tgaaatcggc agtaggttcg
     1381 agtcttttgg caaggatctc tcctatcttg ccgcctccga ggatggtaat acggcgtatc
     1441 tctatctcct ccttaccgga ataggacatt atttcgctca cagcttcatt gagcgctatg
     1501 atgtagacta ggtcgccaac cttgaatata gtctcatgct gcgggattat cgtctcgtct
     1561 ccacgagcta tggcaaccgt tctgaaagga aggttgtcag cgggatagtc gaaatctgcc
     1621 aaagattgtc ctacaagcgg tgcgccctct tccagtttta tcactaccag ttgaagacgc
     1681 ccgtttgcaa aacctacaaa gtctgacaac tctgacttct tcaacaggtc ttcaatctca
     1741 tccgcagcac ttttttcggg gtaaaaaagc agatcaatac ccagctcttt gaagaaaaat
     1801 ttgttctgat aatcgaggta ctcatcatta ttgatgcgtg ccgtgactct tttggcgccc
     1861 atctttttgg ctaaaagcgc cgaaatgata ttgatattct gatcttttgc agggcttaca
     1921 gccacaaaaa ggtctgcatt ctctactcc
//
LOCUS       DAOSIE010000275         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_50603_length_1949_cov_2.296727, whole genome shotgun sequence.
ACCESSION   DAOSIE010000275 DAOSIE010000000
VERSION     DAOSIE010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50603_length_1949_cov_2.296727"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..>683)
                     /locus_tag="PLI69_06895"
     CDS             complement(13..>683)
                     /locus_tag="PLI69_06895"
                     /inference="COORDINATES: protein motif:HMM:NF027696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PL29 family lyase N-terminal domain-containing
                     protein"
                     /protein_id="HOG42247.1"
                     /translation="TPVISVKKAEDGIFYWAVSYSVGHEEFLTNNVGQRIPAVAVTPK
                     IKIENGNWMVTYDNGVIWHNLGHATGSAGTSFIRDVTENDGYVRFNFLDGTYISIPTL
                     EAYDRLNEIVNRTNSNLSSLEQLVTLVSQGVYAKEIKPIIEERDTIGYNLFLSDNTVM
                     TFYNGVATTLPTIGAAKDHLNPADSNYYWTIKYESDREARWILLGGKKVRVSPQAGTL
                     PLVD"
     assembly_gap    684..693
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<694..950)
                     /locus_tag="PLI69_06900"
     CDS             complement(<694..950)
                     /locus_tag="PLI69_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42248.1"
                     /translation="MKRRLSSILTSIVLVLSLTGCFNQIENELGHISRRIKAMEDNLV
                     LMNENIGTLHTLVDKFNSYDFVTRITRIYDNYEVIGYTISFS"
     gene            complement(954..>1949)
                     /locus_tag="PLI69_06905"
     CDS             complement(954..>1949)
                     /locus_tag="PLI69_06905"
                     /inference="COORDINATES: protein motif:HMM:NF027696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PL29 family lyase N-terminal domain-containing
                     protein"
                     /protein_id="HOG42249.1"
                     /translation="APQLKVKDERWLISYDEGKSWVDLGQATGEHGDSMFKSVVISSD
                     HVIFTLTNGTVFRIPVYGKYLALKTEAERINSNVNAQKTIIRAIASKVVYIKNAGDLM
                     ENGERIGTYCNLSNGESFKVYDWQSSNAPKVFSVLDTITGLYYWTFQQNGEEMEWLRD
                     AAGNRIRSMGDTIPPPKISIKVDDRGFFYWTIEYEGGNIVTTEAPQLFVNRTASIFRK
                     VDLSNPDFVLFITWDGAQYQIPKEFTISMETNISMAVKSVKNLEYTVYGADYSEVKAA
                     FITQGGFKAYLSDTLGFVTIESPNDFNSEHGKIMAVFTVKNSQRSSVKTINVNKL"
BASE COUNT          492 a          433 c          422 g          592 t
ORIGIN      
        1 caatgtctcg ttttagtcca ccaacggcaa ggttcccgct tgagggctta ccctcacttt
       61 tttgccacca agcaaaatcc agcgtgcctc ccggtcactt tcatatttga tagtccagta
      121 gtaattggaa tccgcaggat tgagatggtc ttttgctgct ccgatagtcg gtaaagtggt
      181 ggccactccg ttgtagaatg tcataaccgt gttgtcggag aggaaaaggt tgtagccaat
      241 ggtatctcgc tcctcaatta ttggtttaat ctctttggca tatactccct gactgacaag
      301 agttaccaat tgttccaaag atgaaaggtt ggagttggtc ctattgacta tctcgttcaa
      361 tctgtcataa gcctcgagtg tgggtatgga aatataggtg ccgtcgagga aattaaacct
      421 tacatagcca tcattttcgg taacatctct gatgaatgac gtgcctgcac ttcctgtcgc
      481 atggccaagg ttgtgccaaa taactccatt gtcgtaagtc accatccaat tgccattttc
      541 aattttgatt ttcggggtaa ctgctacggc aggtattctt tgtccgacat tattggttaa
      601 gaactcctca tggcctaccg aataggagac agcccaatag aatattccgt cttcagcttt
      661 ctttacactt ataacggggg tctnnnnnnn nnngagaagg atatggtata gcctattacc
      721 tcataattgt catagatacg tgtgattctg gtcacaaagt cgtaactgtt gaacttatct
      781 acaagcgtat gcagggtgcc gatattctca ttcatcagaa cgagattatc ctccattgcc
      841 ttgatacgcc tgctaatatg gccaagctca ttctcaatct ggttgaaaca tcctgtgaga
      901 gataaaacca gcacgattga ggtcagaatt gatgataatc tgcgtttcat actctacaat
      961 ttgtttacgt taatagtttt tacagagctt ctctggctat tttttacagt aaaaacagcc
     1021 ataattttac catgttcgga gttgaagtca ttaggagact ctatcgttac gaaccccagg
     1081 gtatcggaga ggtaggcctt gaatcctccc tgcgttatga atgctgcctt gacttcggaa
     1141 tagtcggcac catacacggt atactccagg ttcttgacgg atttgaccgc catcgaaata
     1201 ttggtttcca ttgatatggt aaactcttta ggtatctggt attgggcacc atcccaggtt
     1261 atgaaaagta caaagtccgg gttggaaaga tccactttcc tgaaaatgga ggcagttcgg
     1321 ttgacaaaga gttgaggagc ctctgtggta actatattgc ctccttcata ctcgattgtc
     1381 cagtagaaga agccgcgatc atcaacctta atgctgattt ttggaggggg tatggtatct
     1441 cccatactgc gtattctatt gccggcggca tcacgcagcc attccatctc ctctccattt
     1501 tgttggaaag tccagtagta gagtccggta atggtgtcaa gtacggagaa gactttgggc
     1561 gcattgctgc tttgccaatc atagacctta aagctctccc cgttagaaag gttacaataa
     1621 gtaccaatgc gctcaccatt ttccattagg tctccggcat tcttgatgta gacaacttta
     1681 gaggcaattg cgcggattat ggttttctgt gcattgacat tgctattgat cctttcggcc
     1741 tcagtcttaa gtgccaaata tttaccataa acggggatcc tgaataccgt accatttgta
     1801 agggtgaata taacgtgatc ggaactgata acgacacttt taaacattga gtctccgtgc
     1861 tctcccgtag cctgtcccag gtcaacccac gatttgcctt catcgtatga gatcaaccat
     1921 ctttcatcct tgactttcaa ttgaggggc
//
LOCUS       DAOSIE010000276         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_50803_length_1944_cov_2.908417, whole genome shotgun sequence.
ACCESSION   DAOSIE010000276 DAOSIE010000000
VERSION     DAOSIE010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50803_length_1944_cov_2.908417"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PLI69_06910"
     CDS             complement(<1..1160)
                     /locus_tag="PLI69_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42250.1"
                     /translation="MKQIVNIIFALVALCSALNAQNRVTICQDEALRPIGMMNGVGGG
                     PSGIVTKELYQAAGIPFARLHDIGNSERHCVEIMNIFRDFDADETKAENYDFTMTDFY
                     IKKIIDSGTKIIWRLGNTGHEPTAVKKYGAWPPKDFKKWARICEHIVRHYNNGWADGF
                     HYNIEYWEIWNEPDGDQKPIVNGKPRYQVSPHSWGGTIEQYFDLFEITFKHLKKTHPN
                     LKIGGPAFAGMWANEMFMEHMNKVGVKPDFYSWHTYARNPERVVEKGAIIREQLDNHG
                     WNDVISVLDEWNYNTTWNEVGAKYSREVRRSVKGAAFVASTMSAMQNAANTDILTYYD
                     FRPNTSYNGAFDKETTKETWTYYVFTAWKKLRDYGTQVKVDLEGDLKDIYTIA"
     gene            complement(1169..>1944)
                     /locus_tag="PLI69_06915"
     CDS             complement(1169..>1944)
                     /locus_tag="PLI69_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42251.1"
                     /translation="SQDLKDLQLFDGFCYYTDKSNEGHIFMVYDIARDEEGNIKEIVT
                     LEEQTPIIKMTKYTPATLRNRIDKISVPVYIYQLSQKYILPDFMEKNLNEIDMNFPDA
                     LCPARGNKSTFGFGKEVEINILSKEYESIELYRNDALIQTDKISGETMKYNYLAAGQY
                     KARLIGSGKASSFAYFEVCSLSYTVKLEGRRLYINCPDGTTPSFMTINDDAIIHALPF
                     KRTASGKWYYDGDLGNATTCRVRFAGKYGTYMANSVSIK"
BASE COUNT          491 a          435 c          383 g          635 t
ORIGIN      
        1 gcaatagtat aaatgtcttt caggtcacct tcaagatcga ctttaacttg agtgccgtag
       61 tcccttagtt ttttccaagc cgtgaacaca tagtaagtcc atgtttcttt tgtggtttct
      121 ttgtcaaacg ctccgttgta agaggtgttc gggcggaaat cgtaataggt aaggatatct
      181 gtgtttgctg cattctgcat ggcgctcatg gttgaggcaa caaatgctgc accttttacg
      241 ctgcggcgca cttctctgct atactttgct ccaacctcat tccaggtggt gttataattc
      301 cattcatcca aaacagagat tacatcgttc caaccatgat tgtcaagttg ctctctgata
      361 atcgcacctt tttcaaccac cctttcagga ttgcgggcat atgtgtgcca tgaatagaaa
      421 tccggtttta ctccgacctt attcatatgc tccataaaca tttcatttgc ccacatgcct
      481 gcgaatgccg gaccgcctat cttaagatta ggatgagtct ttttaagatg cttgaatgtt
      541 atttcgaaca aatcgaagta ttgttcaatg gtgcctcccc aagaatgagg agagacttgg
      601 taacgcggct ttccgtttac tatcggcttt tgatcgccat cgggctcatt ccagatttcc
      661 caatactcta tattatagtg gaaaccatca gcccaaccat tattgtaatg ccggactata
      721 tgctcacaga tacgtgccca ttttttaaaa tcctttggag gccatgcgcc atatttcttg
      781 acagcggtag gctcgtggcc tgtattgcct aagcgccaaa taatcttggt gccggagtca
      841 atgattttct ttatgtagaa atccgtcatc gtaaaatcgt aattctccgc ctttgtttca
      901 tcggcatcaa aatcgcggaa tatattcata atttccacgc aatgacgttc gctattgcct
      961 atgtcgtgca atcgggcaaa tgggataccc gccgcttggt agagttcctt tgtcacaatt
     1021 ccggaagggc ctccgccgac accgttcatc atcccgatcg gtcttaaagc ctcatcctgg
     1081 caaatagtca cacggttctg tgcgtttaat gcagagcata gtgcgaccaa tgcgaatata
     1141 atattaacta tttgtttcat tcgattaact atttgatgct tacactatta gccatatatg
     1201 ttccgtactt gccggcaaat cttacacggc aagttgtagc atttccaaga tctccgtcat
     1261 aataccattt tccactggca gtacgtttga atgggagtgc atgtatgatt gcatcatcgt
     1321 tgatggtcat gaatgaggga gtcgtaccgt cagggcaatt gatgtagagt cttctgcctt
     1381 ccaatttgac tgtatagctg agactgcaga cttcgaaata tgcaaatgaa gaagctttgc
     1441 cgcttcctat caatcttgct ttgtactggc cggcagccag atagttatat ttcatggttt
     1501 ctccagatat cttatccgtc tggattaaag catcatttcg gtatagttca atcgattcat
     1561 attctttaga gaggatatta atctcaactt cttttccaaa gccaaaagtg cttttattcc
     1621 cccttgccgg acaaagagca tcaggaaagt tcatatcaat ctcgttcagg ttcttttcca
     1681 tgaaatcagg aagaatatat ttttgcgaca attgatatat ataaaccgga acggagattt
     1741 tatcaattct atttctcaag gtggccggtg tatattttgt catcttgatt atcggtgttt
     1801 gttcctctaa agttacaatc tctttgatat tgccctcttc atctctggca atgtcataaa
     1861 ccataaagat atgcccctcg ttgcttttgt cagtgtaata gcaaaatcca tcaaagagtt
     1921 gtaagtcttt caggtcttgg ctct
//
LOCUS       DAOSIE010000277         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_50944_length_1940_cov_3.045093, whole genome shotgun sequence.
ACCESSION   DAOSIE010000277 DAOSIE010000000
VERSION     DAOSIE010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50944_length_1940_cov_3.045093"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..513
                     /locus_tag="PLI69_06920"
     CDS             145..513
                     /locus_tag="PLI69_06920"
                     /inference="COORDINATES: protein motif:HMM:NF025660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HOG42252.1"
                     /translation="MKRIVLLISFITLAMVIIGCNREEDPNWSERAEIIVSHKTTDYW
                     PGEYPPELPPMVGISIRKINETAWTKLPIDGISGFTYESGYEYHLKVKITHLADPPMD
                     GPNRTYESLKIISKTQKLIE"
     gene            519..1829
                     /locus_tag="PLI69_06925"
     CDS             519..1829
                     /locus_tag="PLI69_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOG42253.1"
                     /translation="MKKITTLLFILIAALGFSACEKSDVPRVKNPIEIDFPTSTTPIT
                     KVTLSEEQKSYVNSGNAFALKSLRSLYEKEKNSIIYSPLGLQFALAITANGASGETAS
                     EIIGTLGYGEDQEALNKYCNLLLNQLPALDKDVELKLTDAVIINEEFRAQDAFRKTAE
                     KMYYAPVEYVNASNPKVVVDRINEWAYRSTNGFIFPFLQESDIKRDFVAAILNALYFK
                     ARWSGNRDIPMFNSELTKKAQPFYLDGGGESKVDYMVTSRHFRHGRVGNNGILELPYA
                     GGKFAMYIILPDIKGGNGLEQLFSYITSEKLSEAISSISTTEAMVHLNLPKFEVENKY
                     ELTDMLKALGIRRAFNDDAQFDKLLYHPEIKNFNIGSVIQKARISVAEQGTEAAASTA
                     VMIERTSSAGQPKMIEFFADHPFVYTIVEKSSGVMLFAGVYDGK"
BASE COUNT          607 a          361 c          403 g          569 t
ORIGIN      
        1 tattaattgc tcgtttacga cctttggtta tcccctggta gaagatatcg ggtatcattt
       61 ttcactgact tctgatattt gtaaaaacgt ttattgttgg tcgccttcag gggctctttg
      121 aaaaatttta aaataatatt tcctatgaaa cgaatagtac tgcttatttc atttataaca
      181 ctcgccatgg taattatcgg ctgtaatcgg gaagaagatc cgaattggag cgagcgtgct
      241 gaaatcattg tttcacataa aacaacagac tattggccag gcgaatatcc tccggaactt
      301 ccaccaatgg tgggaatatc cattcgcaaa atcaatgaga cggcatggac aaaactgccg
      361 atcgatggaa tatccggatt tacctatgaa tcgggatatg aataccatct taaagtgaaa
      421 ataactcatt tggcagatcc acctatggat ggaccgaata gaacctatga gtcgcttaaa
      481 attatttcaa agacacaaaa actaatagaa taatcattat gaaaaagatc acaaccctat
      541 tatttatttt aattgctgct cttggtttct cagcatgtga aaagagcgat gttccaaggg
      601 taaagaaccc catagaaatt gattttccca cttctacaac tccaatcacg aaagtgacat
      661 tgagtgagga gcaaaaaagc tatgtcaact ccggtaatgc ttttgcccta aagagcttga
      721 ggtctttata tgaaaaggag aaaaacagca taatttattc tccactcggc ttacagtttg
      781 ctcttgcaat tactgcaaac ggtgccagcg gcgagacggc ttccgagatt atcggaacct
      841 tagggtatgg ggaagatcag gaagctctga acaagtattg taaccttttg ctcaatcaat
      901 tgcctgcact tgacaaagat gtcgaattga agttgacaga tgcggtgatt ataaatgaag
      961 aatttcgtgc tcaggatgct ttccgcaaaa cagccgaaaa gatgtactat gccccggtgg
     1021 aatatgtgaa tgcttcaaat ccgaaggttg ttgtcgacag gataaatgaa tgggcatatc
     1081 gcagtaccaa cggttttatc tttccatttc tacaggaatc ggatattaaa cgagattttg
     1141 ttgcagcaat actcaacgcg ctatatttca aggcaagatg gagtggaaat agagatatac
     1201 caatgttcaa tagtgaatta acaaagaaag cgcagccctt ctatctggat ggcggaggag
     1261 aaagtaaggt cgattatatg gttacttcaa ggcacttccg tcatggaaga gtgggaaaca
     1321 atggaatctt agagcttccg tacgctggtg gtaagtttgc aatgtatatc attctgcctg
     1381 acataaaagg tggcaatggt ttggagcaac tattctcgta tatcacttca gagaaattgt
     1441 ctgaggccat ctcttcaatc tctactacgg aggccatggt acatctcaat ctccccaagt
     1501 ttgaggttga aaataaatat gagcttacag atatgttaaa agccttaggt attcgtcggg
     1561 cttttaatga cgatgctcaa ttcgataaat tgctttatca tcccgaaatc aaaaatttca
     1621 atattgggtc agttattcaa aaggcacgta tatctgttgc tgaacagggt actgaggcgg
     1681 cagcgtccac tgcggtaatg attgagagga cctcttccgc cggtcaaccc aaaatgattg
     1741 aattctttgc agatcatcct ttcgtctata ccatagtaga aaaatcatcc ggcgtaatgc
     1801 tttttgcggg agtctatgat ggaaagtaaa aacagttgat atcctaataa attattgaga
     1861 ccggctaata ttttagtcgg tctcattttt atttgtagaa atcttattgc tctaaatcaa
     1921 agaaataaat atttctcttt
//
LOCUS       DAOSIE010000278         1934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_51160_length_1934_cov_3.788185, whole genome shotgun sequence.
ACCESSION   DAOSIE010000278 DAOSIE010000000
VERSION     DAOSIE010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51160_length_1934_cov_3.788185"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..559)
                     /locus_tag="PLI69_06930"
     CDS             complement(158..559)
                     /locus_tag="PLI69_06930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOG42254.1"
                     /translation="MASIFTRIVNGEIPSYKVAEDDRFYAFLDINPLAEGHTLVIPKI
                     EVDYIFDLDEETYRGLFAFARKVAIAQKSVISCKRIGMAVLGMEVPHAHIHLVPLRTE
                     GDLDFRKKKKSLTPEQFKEIAASISNAFNKI"
     gene            complement(568..1041)
                     /gene="greA"
                     /locus_tag="PLI69_06935"
     CDS             complement(568..1041)
                     /gene="greA"
                     /locus_tag="PLI69_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOG42255.1"
                     /translation="MSTEINYVTREGLQKLREELAQLFAQRPVISRQIADARDKGDLS
                     ENAEYDAAKEAQGLLELKIAKMESLIANARVIDESKINTDEVQMLNKVKIKNLGNNSI
                     VEYTLVGESEANFKEGKLAAATPIGKALIGRRKGDIVEAVVPSGILKFEILDISI"
     gene            complement(1128..1577)
                     /locus_tag="PLI69_06940"
     CDS             complement(1128..1577)
                     /locus_tag="PLI69_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42256.1"
                     /translation="MDLDILVGVMVFFTAMMFAAINGSKKKQQQSGKTGHMGLPDTNS
                     RPPRPTRPNSPQGALEELRRLLEVPDEHSSEVEDGVETYMEPESVVATPVEVGAEEGV
                     RSTVTIKEEQYSLDKKNNESAKGVKVEIEPRNLIIYSEIMRPKWKEY"
     gene            complement(1599..>1934)
                     /locus_tag="PLI69_06945"
     CDS             complement(1599..>1934)
                     /locus_tag="PLI69_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HOG42257.1"
                     /translation="LGRTIGFPTANMQLYEPLKLVPATGVYFVKVEVTGGTFKGICNI
                     GTRPTVSSDTSRRIETHILDFDEDIYGLDIRISFIERLRDERRFGSLEELRQQLLLDK
                     KCCIDKNIF"
BASE COUNT          538 a          464 c          355 g          577 t
ORIGIN      
        1 tcaaatgaaa cttctccatt aaccaagcta tgtgttacat ttttaaaatt cggaaaatct
       61 acatttatca aaggatgtgc taaaagatat tttgaccgag ccaacatcaa ttctcgaatc
      121 cctaccatta cgacactacc agcttttgct gatttttcta tattttgttg aaagcattgg
      181 atatcgaagc ggcaatctct ttgaattgct ccggcgtcaa ggattttttt ttcttcctaa
      241 aatcaagatc tccttccgta cggagcggaa caaggtggat gtgtgcatgg ggaacttcca
      301 tccccagcac ggccataccg attcttttac aggaaattac actcttctgc gctatagcta
      361 ccttacgagc aaatgcaaac aatcccctat aggtctcctc gtccaggtca aaaatataat
      421 caacctcaat tttgggaata acgagagtgt gcccctcagc cagggggttt atgtcgagaa
      481 atgcgtagaa cctgtcgtcc tccgccactt tgtaagacgg tatttcacca tttacaatcc
      541 ttgtaaaaat tgatgccata tctgtgacta tattgaaata tcgagaattt caaactttaa
      601 aatacctgaa ggtactactg cctcaactat atcaccttta cggcgaccaa tgagggcttt
      661 gccgatagga gtggcagcag cgagttttcc ctctttaaag ttggcctcgc tctctccaac
      721 gagagtatac tccacaatgg agttgtttcc aagatttttg atcttaacct tgttgagcat
      781 ctgcacctca tcggtattga ttttagactc atctatgact cttgcatttg caataaggct
      841 ctccattttg gctattttta gctcaagaag cccctgtgcc tctttagctg catcatattc
      901 tgcattttcg gaaaggtcac ccttatctct ggcatctgct atctgtcttg aaatgacagg
      961 tctctgggca aacaattgcg ccagctcttc acgaagtttt tgaagacctt cacgtgtaac
     1021 ataatttatc tccgtagaca tctctataaa tattataaaa aatcaaaaag gaatcccgtt
     1081 tgggatcctt tcatttgtaa atcactccgc aaaaatagtt aaaaaaatca atattccttc
     1141 catttgggac gcataatctc agaataaatg atgagattgc gtggctctat ttcaactttt
     1201 acaccctttg cggactcatt atttttttta tctaatgaat attgctcttc tttaatagtt
     1261 acagtactcc tgacaccttc ctcagcacca acttcaacag gcgtggcaac tactgactcc
     1321 ggttccatat aggtttccac cccatcctca acctctgaag agtgctcgtc aggcacctcc
     1381 agcagtcgcc tcaactcttc aagagcacct tgcggagagt tgggtcttgt aggacgtggt
     1441 ggacgcgagt tagtgtccgg caagcccata tgaccggtct tacctgattg ttgctgtttt
     1501 ttcttactgc cgttaatagc cgcgaacatc atggcagtaa aaaacaccat aactcctact
     1561 aatatatcta aatccataac tcagagattt atacaaagtt agaaaatatt cttgtcaata
     1621 cagcattttt tgtcaagtaa aagttgctgg cgcaactcct ccaaagaacc gaaacggcgc
     1681 tcatcacgca atctctcaat aaaagatatc ctgatgtcca gaccgtatat atcctcatca
     1741 aagtcgagaa tatgcgtctc tatcctacgg ctggtatcgc tggagacagt aggtcgcgta
     1801 cctatgttgc agatgccctt aaaggtacca ccggtaacct caactttcac gaaatagacc
     1861 ccggtggcag gaaccagctt gagaggctca tacagctgca tattggcggt cgggaaacct
     1921 attgtacgac caag
//
LOCUS       DAOSIE010000279         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_51200_length_1933_cov_3.205005, whole genome shotgun sequence.
ACCESSION   DAOSIE010000279 DAOSIE010000000
VERSION     DAOSIE010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51200_length_1933_cov_3.205005"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLI69_06950"
     CDS             complement(<1..598)
                     /locus_tag="PLI69_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOG42258.1"
                     /translation="MSLFIPTGYSNILGTVVRTEKAIKVVKDMFQLNLSAQLSLLRVT
                     APMVVMKGTGINDDLNGVERPVTFPIRDLEDSPAEVVHSLAKWKRLKLATLQTPVGSG
                     IYTDMNALRPDEELDNIHSIYVDQWDWERVIESSERNLDFLKKIVRRIYEAIKVTENK
                     IYVDFPQIYPILPEEIFFIHSEELLQRYPGLSSKEREDA"
     gene            725..>1933
                     /locus_tag="PLI69_06955"
     CDS             725..>1933
                     /locus_tag="PLI69_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG42259.1"
                     /translation="MKTKKKGPEDNINTLGKLYEDSTTNYSDKKLSEIIDGDLSYTYG
                     SFKKKSDSISEKLSKYGIGSGDKVAILSHNTPNWTVAFFACVSFGRIVVPILTDSSEN
                     EVTNMLLHSESKAIFISGKLLHKLSQESLSSVHLIVDIDTFDIIRADKVPFTGNGSVT
                     MPMPDDIAVIIYTSGTTGSAKGIVLSHRNLTFNLKSYHKTYKCTTRDKWLSILPMAHS
                     YELTLGILYPFYCGASVYYMSKPPVPALLLDSMKKIRPTVLLTVPLVIEKIYRNSVLP
                     AIRNSKILSWMNKHQHKLLCRIFGMKLRMTFGRRFGMGIGGARLDPTVEDFFYKARFN
                     YAIGYGLSETSSLICYALGKNRYPGSIGVPLPGVEMRLDNINPKTGEGEILVRGDNVM
                     PGYYKDPERTK"
BASE COUNT          545 a          428 c          388 g          572 t
ORIGIN      
        1 ttgcatcttc tctctccttg gatgagagtc ccggatatct ttgcaataat tcttccgaat
       61 ggataaagaa aatttcttcc ggtagtattg ggtaaatctg tggaaaatcc acatatatct
      121 tattttcagt cacctttatg gcctcataga tacggcggac tatctttttg aggaaatcaa
      181 ggtttctttc ggaactctca attactcttt cccagtccca ttgatccaca tagatagagt
      241 ggatgttgtc cagttcctca tcgggtctca gagcattcat atcagtataa ataccgcttc
      301 cgaccggagt ttggagtgtt gccaacttta agcgtttcca ttttgcaaga gagtgtacaa
      361 cttctgcggg gctatcttcc agatccctga tagggaaggt cacaggacgc tcaactccgt
      421 tgagatcatc gttgatccct gttcccttca taaccaccat tggagccgtc acccgcaaca
      481 gagaaagttg agccgaaagg ttgagttgaa acatatcttt aaccactttt atggcctttt
      541 cggtcctgac gaccgtacca agaatattgc tgtaaccggt gggaataaat agtgacatag
      601 tctgtagtaa gtgttttgag ggttcaaatc tatcccaact ccgacaatta tccaaatatt
      661 tgacaacttt gcgtatcttt ggcaattgta taatcccccg tttacaccag ttatgaggaa
      721 aattttgaaa acaaaaaaga agggtcctga agataatatt aacaccctag gtaaactcta
      781 tgaagattca acaacaaatt atagtgataa aaaactttct gaaataatag acggggatct
      841 ttcctataca tacggttcat ttaagaaaaa gagtgattca atctcagaaa aactcagcaa
      901 atatggcatt ggatccggag ataaggtagc aatcctttcc cacaatactc ccaattggac
      961 ggtagctttc tttgcttgtg tctccttcgg ccgaattgta gttcctattc ttaccgattc
     1021 ttcagaaaat gaagtaacca atatgctgct ccattccgag agtaaggcga tttttatctc
     1081 cggaaaactc ctccataaac tctcacaaga atccctctcc agtgtgcacc tgattgtgga
     1141 tatcgatacc tttgatatta ttagagcgga taaagtgccc tttaccggca atggaagtgt
     1201 aacaatgcct atgcctgatg atatagccgt tatcatctac acttcgggca ctacaggtag
     1261 cgctaaggga atagttttga gccatcgtaa tttaaccttt aacttaaaat cttaccataa
     1321 aacctataag tgtacgacta gggacaaatg gctctcaatt cttcctatgg cccattccta
     1381 cgaacttacc ctcggaatac tctatccatt ttattgtgga gcatctgttt actatatgtc
     1441 caagccgccg gttcccgcac ttctgttgga ctctatgaaa aaaatccgtc caacagtcct
     1501 actaactgtg ccacttgtta ttgaaaagat ctatcgcaat agtgtgctac ctgctattcg
     1561 caatagtaag attctctcat ggatgaataa gcatcaacac aaactgctgt gccgcatttt
     1621 tggcatgaag cttagaatga cctttggccg cagattcgga atgggtattg gtggtgcgag
     1681 gcttgaccct actgttgaag atttttttta taaggcccgc ttcaattatg ctataggcta
     1741 tggccttagt gagacctctt ctcttatttg ttacgcgctg ggcaagaata ggtatcccgg
     1801 atctatcgga gtccctctac ccggagtaga aatgcgtctg gacaatatca accctaagac
     1861 aggggagggg gaaattttgg tcaggggaga caatgtcatg ccaggttatt acaaggaccc
     1921 cgaacgcacc aag
//
LOCUS       DAOSIE010000280         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_51623_length_1922_cov_2.644885, whole genome shotgun sequence.
ACCESSION   DAOSIE010000280 DAOSIE010000000
VERSION     DAOSIE010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51623_length_1922_cov_2.644885"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>230)
                     /locus_tag="PLI69_06960"
     CDS             complement(<1..>230)
                     /locus_tag="PLI69_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG42260.1"
                     /translation="KEESAEIYMALSYIMSFLIYMFIFMFGNMVMRGVIEEKSGRIVE
                     VIVSSVKSVELMIGKIVGVALVALTQFLIWVV"
     assembly_gap    231..240
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<241..763)
                     /locus_tag="PLI69_06965"
     CDS             complement(<241..763)
                     /locus_tag="PLI69_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42261.1"
                     /translation="MNFNKIGIILGREYSVRVKKKSFILTTILTPVLMAALFIVPTVL
                     MVVGGKDTEKVMVQDGSGIVVPYLESSSTVEYVVAPEGSTLEQLKADFNYLGVYALVG
                     ISELDSANNVQVVAYSAEPLNVDIKSDISRKVNKAVESMKLSLYNISDLDRILAEVKS
                     DVQITTLTLTDEGE"
     gene            complement(773..1675)
                     /locus_tag="PLI69_06970"
     CDS             complement(773..1675)
                     /locus_tag="PLI69_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG42262.1"
                     /translation="MLECRSVTKKFGDFKALDNVSIEANQGRVFGLLGPNGAGKTTLI
                     RIINRITIPTEGEVIYNGRLMNDEMVREIGYLPEERGLYKKMKVGEQAIYLARLKGLS
                     MAEARKELKEWFVKFGIRSWWDKKVEELSKGMAQKVQFITTVLHKPSLLILDEPFSGF
                     DPVNVQLIRNEILRMKDEGATIILSTHNMESVEELCDDISLINHAKVVLSGPTGQIRK
                     EYGTNQVEVLYRSSQPLATWSKYFSISSDEEYKTLRKAVLDVGEGAGSHQIIPELLGS
                     VDIVSYRELVPRMNDIFIKLVSEK"
BASE COUNT          527 a          454 c          370 g          561 t
ORIGIN      
        1 caccacccat atcaggaatt gggttaaggc aacgagggca actcctacaa tcttaccgat
       61 catcaactcc accgacttga cggaggagac aataacttca actatacgac cgctcttctc
      121 ctcaatcact ccacgcatca ccatgttgcc gaacataaag atgaacatat agatcaaaaa
      181 gctcataata taagagagcg ccatataaat ttccgccgac tcctcctttt nnnnnnnnnn
      241 tttctccctc atcggtgaga gtcagtgttg taatctgtac atcgctcttt acctctgcga
      301 ggattcggtc aagatcggag atattgtaaa gtgagagttt catcgactcc acggctttat
      361 tgactttgcg tgaaatatcg ctcttgatat ccacattgag aggttcagcg gagtatgcca
      421 ccacctgtac gttattcgcg gagtcaagct ccgagattcc tacgagagca tagacaccaa
      481 gataattgaa atctgccttt agttgctcaa gagtgctgcc ttcaggcgca actacatatt
      541 ctaccgtgga actgctttcg agataaggaa ccactatgcc tgaaccgtcc tgtaccatga
      601 ccttctccgt atccttgccg cctaccacca ttaacacggt aggcacgatg aagagagcgg
      661 ccattagcac cggagtaaga attgttgtta gaataaagga ttttttcttg acacgtaccg
      721 aatattcgcg tcccagtatt atacctatct tattgaaatt catggctccc tcctattttt
      781 cgcttacaag tttgataaaa atgtcgttca tgcgtggaac gagctcccta taagagacga
      841 tatccacgct acccagcaac tccggaatga tttggtgcga tcccgctcct tcgcctacgt
      901 caagtacggc tttccttaaa gttttgtatt cttcatccga agagatggaa aaatatttgg
      961 accaggtagc aaggggctgg gaggagcgat agagaacctc aacctgattg gtaccatatt
     1021 cctttcgtat ttgacctgta ggacctgata aaacaacttt agcgtggttt ataagcgaaa
     1081 tatcgtcgca aagctcctct accgattcca tgttgtgagt ggaaagaatt atggtagccc
     1141 cctcatcttt catccttaaa atctcattcc tgatgagctg cacattcacc gggtcaaaac
     1201 ctgaaaaggg ttcgtccaga atcagcaatg agggtttgtg gagcactgtg gtgataaatt
     1261 gcaccttctg ggccataccc ttggagagtt cctctacctt cttatcccac caactgcgaa
     1321 taccgaattt tacaaaccac tctttgagct ccttcctggc ctctgccatt gagagacctt
     1381 ttaggcgtgc aagatagatt gcctgctccc ctaccttcat ttttttgtag agaccgcgtt
     1441 cttccggcag ataaccgatt tcgcgaacca tttcgtcgtt catcaatctg ccgttataga
     1501 ttacctcgcc ttcggtaggg atcgttatgc ggttgataat acgtatgaga gtggtcttac
     1561 cggccccgtt tgggcccagc agaccaaaaa cacgcccctg attagcctct atcgagacat
     1621 tatcaagagc cttgaagtca ccgaattttt tggtgacgga tctgcattcc aacatgtttt
     1681 actgttttta acaaaaatcg cgtgtaaagg taaacaaaaa aatcgtctgt cccctgtaat
     1741 aaatattatt tatgatattc ctttactgga aaattattta atttacatgt tatctaatta
     1801 taataaacct cagaatatca atagcgaaat aatatcttat attattaaaa acacatcttg
     1861 gggatttaat tatctacttg ctaattttaa tttgctgttc agaaaaattg attatgattc
     1921 tg
//
LOCUS       DAOSIE010000281         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_51955_length_1914_cov_2.293706, whole genome shotgun sequence.
ACCESSION   DAOSIE010000281 DAOSIE010000000
VERSION     DAOSIE010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51955_length_1914_cov_2.293706"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>499)
                     /locus_tag="PLI69_06975"
     CDS             complement(<1..>499)
                     /locus_tag="PLI69_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOG42263.1"
                     /translation="TDEKFNERALNFALFKNTDGKYFNYEDYRKAIEKEQTNKEKKVV
                     YLYATDAEGQYQYIETAKNRGYDVLLFDSPLDSHFVSMLEQKLENSTFARVDADSIDK
                     LISKSDVEKYEVSEEEQKRLDALFEEILPKENKYRVSVENLGDTAQPVLITQTEFLRR
                     YRDMAA"
     assembly_gap    500..509
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<510..1643)
                     /gene="htpG"
                     /locus_tag="PLI69_06980"
     CDS             complement(<510..1643)
                     /gene="htpG"
                     /locus_tag="PLI69_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOG42264.1"
                     /translation="MAKGTIGVTTENIFPVIKKFLYSDHEIFIRELVANAVDATQKLN
                     TIASSGEFKGELGDLTIRIELDPEAGALKITDRGIGMTAGEVDKYINQIAFSSAGEFL
                     EKYKEQAAGIIGYFGLGFYSAFMVSKKVTIDTLSWKEGSKAVRWECEGDPEYTISDSD
                     KQDRGTTITLHLDEESKEFAQEHKISGLLHKYCKFMPVPITLGKKKEWKDGKYVDTDE
                     DNIINDVKPLWTLKPTDLKDEDYMEFYRELYPMQDDPLFYIHLNVDYPFNLTGILYFP
                     KIKNQIDVARNKIQLYSNQMFVTDSVENIVPEFMTLLHGVIDSPDIPLNVSRSYLQSD
                     SNVKKISGYITRKVADRLEEISKNNKEEFESKWDNLKLFIEYGM"
BASE COUNT          477 a          409 c          415 g          603 t
ORIGIN      
        1 gtgcagccat atcacgataa cgccttagga actcggtctg cgtgataagc acgggttgag
       61 ccgtgtcgcc aagattttct acagatactc tgtatttgtt ctctttggga agaatctcct
      121 cgaaaagagc atcgagacgc ttctgctcct cctcacttac ctcatatttc tcaacatctg
      181 atttagagat gagcttgtcg atggaatcgg cgtcaacacg ggcaaaagtc gaattttcca
      241 gcttttgctc cagcattgag acaaagtgcg aatccagtgg agagtcaaat aataacacgt
      301 cgtaacccct gtttttagcg gtttcaatgt attggtactg tccctcggca tctgtagcat
      361 agagatagac tactttcttc tccttatttg tctgctcctt ttcgatggcc ttgcggtaat
      421 cttcataatt gaaatatttg ccgtccgtat tcttgaaaag tgcaaaattg agagctcttt
      481 cgttgaattt ctcgtcggtn nnnnnnnnnc attccatact ctataaatag cttgagattg
      541 tcccatttcg actcaaattc ttccttattg ttttttgaaa tctcctcgag acggtcagct
      601 accttcctgg taatataacc ggaaatcttt tttacattgg aatctgactg gaggtagctc
      661 cttgagacgt taagagggat atcaggagag tcaattactc cgtggaggag tgtcataaac
      721 tcgggtacga tattctccac cgagtcggta acgaacatct gattgctgta gagctgtatt
      781 ttattacggg ctacgtcaat ttggttttta atcttgggga aatagagaat acctgtcaga
      841 ttgaagggat agtccacgtt tagatgaata tagaagagcg gatcatcctg catgggataa
      901 agttcacgat aaaactccat ataatcctca tccttgagat ctgtaggttt tagtgtccat
      961 agaggcttaa cgtcgttgat gatattatcc tcatcggtat ctacatattt tccatctttc
     1021 cactctttct ttttacccag agtaatggga accggcataa acttgcagta tttgtgcagc
     1081 agtccactga ttttgtgctc ctgagcgaac tctttggact cctcgtccag gtgaagtgtt
     1141 attgtagtac ctctgtcctg tttgtcggaa tcacttattg tgtactccgg atcaccctcg
     1201 cactcccatc tgactgcctt ggaaccctct ttccatgata aagtgtcaat agtcaccttt
     1261 ttggaaacca taaaggctga atagaagcca agaccaaagt agcctataat accggcggcc
     1321 tgttccttat atttttccag gaattcacca gcgctggaaa atgcgatctg gttgatatat
     1381 ttatctacct ctccggccgt cattcctata ccgcggtctg tgattttcag ggcaccggct
     1441 tcgggatcca attcaatcct gatggtcaag tcacccaatt cacccttaaa ttctccgctg
     1501 gaagcgatcg tattgagctt ttgagtggca tccactgcgt tagccacaag ctcgcgtata
     1561 aatatttcgt gatctgaata aagaaatttt ttaattaccg gaaatatatt ctcggtagtt
     1621 actccaattg ttccttttgc cataatctta tattaaaact gtttagaatc tttgttttaa
     1681 ccaagaatgg gcaaaaagag taccatcccc cgaaactgac aaaatgtctg tttcgggtta
     1741 gttctgagtt atgagtgtgt agtatgtagt attgcgtggt gcgatttggt gcgcagtacg
     1801 gtgtgcgagt tgcaagttgc ggggtacgag gtggtagcga ggtacgggtt gcgtggtact
     1861 agttcgcggt agctaacagc taacggccaa tagctaatgg ctaatggcta atgg
//
LOCUS       DAOSIE010000282         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_52191_length_1907_cov_3.294816, whole genome shotgun sequence.
ACCESSION   DAOSIE010000282 DAOSIE010000000
VERSION     DAOSIE010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52191_length_1907_cov_3.294816"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..988)
                     /gene="murI"
                     /locus_tag="PLI69_06985"
     CDS             complement(209..988)
                     /gene="murI"
                     /locus_tag="PLI69_06985"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOG42265.1"
                     /translation="MIGIFDSGVGGLSVWREIFRLMPGESYLYISDGAFCPYGPKDPE
                     FIRERSAKISAYLIGKGAEIIVVACNTATAAAISSLRENFALPFVGMEPAIKPAALHS
                     KSGVVGVLATAGTFRGRLYLDTLARFAGDVRVVEQVGEGLVEAIERGVFDSPAIYNLL
                     HHHIDGMVAAGADHIVLGCTHYPFVEEAIAKIAGPEVVIVNPAPAVAAQTMKVLSQIR
                     EISGGIGESFFYSTGEISVLKMLVRHEIPDMTDSHFTEILI"
     gene            complement(1093..>1907)
                     /locus_tag="PLI69_06990"
     CDS             complement(1093..>1907)
                     /locus_tag="PLI69_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG42266.1"
                     /translation="VAATVMGLRARVALAMHDWANAKKYAEMAINTTSAKLMSQSDYT
                     TGFNDASNVEWMWAFHMIPDQTLYFYGYMAYMSINFNSSNIRSCPKCINKNLYDPLPA
                     TDIRKTLWDPTGKAFEVPNTRYTKKPYMNRKFQVLDPSSSVADHSYMRLGEMYLIAAE
                     ACAKLNEFGAAQTHLYTLNKARDNAYVKSSKTGKDLLDEIYFYRRIELWGEGHRFLDL
                     KRLGLALDRRNSNHDAAVSTEMYVAPTDPRWKFSIPQEEIDANDLIPKNIND"
BASE COUNT          528 a          435 c          415 g          529 t
ORIGIN      
        1 gtgtacaatg taatctatag tacaattata taaagtgttg tgttgtatag tatagtatag
       61 tatagtatag tgtagtgtag cttgattagg ttagtgtgga ttcgtgcagc gtactgtaac
      121 gtagtgtaca atgtaatgta tagtgcaatt ttataaagtg ttgtgttgta tagtgtagtg
      181 tagcgtagta ttggagtgat tttatatttt aaattaaaat ttcagtaaaa tgagagtcag
      241 tcatatctgg tatttcatga cgcaccagca tctttagtac acttatttct cctgtagagt
      301 agaaaaatga ctcccctatt ccacctgata tctcccgaat ctgagagagt actttcatag
      361 tctgcgcagc aactgccggt gcaggattga caatcacaac ttcgggacct gcaattttgg
      421 ctattgcttc ctctacaaaa ggataatggg tacatcccaa aactatatgg tcggctcccg
      481 cagccaccat tccatctata tggtggtgca gtagattgta tattgccgga gaatcgaaaa
      541 cacctctctc aatcgcctct acaagcccct ctcccacctg ttcaaccacc cgcacatctc
      601 cggcaaagcg tgccagcgta tcaagataga gtcggcctct gaatgtccct gcggttgcaa
      661 gaacccctac tacgccactt ttcgagtgca atgcagctgg ttttatagcc ggctccatcc
      721 ccacaaaagg gagagcaaag ttttccctca gtgaactgat cgcagccgct gtagctgtat
      781 tacaggcaac cacgataatt tcggctcctt tgccgataag ataggcagat attttagccg
      841 acctctccct gataaactcc ggatcttttg gcccataagg gcaaaaagcc ccgtcagaga
      901 tatagagata ggattcaccc ggcataaggc gaaatatctc acgccaaacg gataagccac
      961 ctactccgga atcgaaaatg ccaatcatgc gtcaaaggta agtaaaaatc cgcctctttg
     1021 tataacaaaa gccccgaaag tatcaacttt cggggccgtt tgtgtcggac gtactgtccc
     1081 gagaattaac aattagtcat taatattctt aggaatcaag tcgttagcgt caatctcctc
     1141 ctgagggatt gaaaacttcc agcggggatc ggtaggcgca acatacattt cagtagaaac
     1201 cgcagcatcg tgattagagt tacgtctgtc aagagccaga ccgagacgtt tcaggtcaag
     1261 gaaacgatgt ccctcacccc aaagttcgat tcttctgtag aagtaaattt catccagaag
     1321 atctttaccg gtcttgctgc tctttacata agcattatca cgtgccttgt tgagagtgta
     1381 gagatgtgtc tgggcagctc caaactcatt gagtttcgca caagcctcag ctgctatcag
     1441 atacatctca ccaagtctca tgtagctgtg atcagctacc gaacttgaag ggtcaagaac
     1501 ttggaacttc ctattcatat aaggcttttt ggtatacctc gtgttgggta cctcgaaagc
     1561 cttgccggta ggatcccaaa gggtcttacg aatatccgta gcagggagtg gatcataaag
     1621 atttttgttt atacacttgg gacaactcct gatgttggag gagttgaagt taatggacat
     1681 ataagccata tagccgtaga agtagagtgt ctggtcggga atcatatgga atgcccacat
     1741 ccactctaca ttggaagcat cattaaagcc agtcgtgtaa tcactctgac tcatcagttt
     1801 tgcgctggtg gtattgattg ccatctctgc atacttcttg gcattagccc agtcatgcat
     1861 tgccaaagca acacgtgccc tgagacccat taccgtagca gccacaa
//
LOCUS       DAOSIE010000283         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_52732_length_1893_cov_3.169206, whole genome shotgun sequence.
ACCESSION   DAOSIE010000283 DAOSIE010000000
VERSION     DAOSIE010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52732_length_1893_cov_3.169206"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PLI69_06995"
     CDS             complement(<1..937)
                     /locus_tag="PLI69_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG42267.1"
                     /translation="MCKFRKHREVYEKDPLYTILRPSVDAVIKGSYQKIIFHNTDRIP
                     RDSAVIFTPNHCDALMDPLLVLAANRDRKVFIARADIFKNPILRKILTFFKMIPINRI
                     RDGIRTVTAVEQTIRASIKVLKHNVGLCILPEGTHRTMHSLLPIGKGIARIASGAYDA
                     LGDDHPIYIVPVGVEYGDYFRFRSTAICNIGEPFNVSEFISSMRKDGASERDIMEAIR
                     QQIKERLQELIVYIDDDDKYEAVWELAKLHSGPICKYRVMERFKANKEIISRIKQLRE
                     NEPKKASKLLEKADKLRERRLKARISIHAIHRKHPL"
     gene            complement(930..1892)
                     /locus_tag="PLI69_07000"
     CDS             complement(930..1892)
                     /locus_tag="PLI69_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOG42268.1"
                     /translation="MNLDPVKINFSKAGMDMINFVLAFVMYGVALGIKPSMFKEVFKK
                     PKSVILGAICQMVLLPALTFLLVIILNDRITPMVAMGMILVAACPGGNISNFMSQLSK
                     ANAEFSISLTAISTSLAPLTTPGNFALWGGLYVRYLNKHAAGALQPLEIPISDVMVTV
                     FILLGIPLILGLLTNKYLPKVSEKLKKPMQIFSILFFLLMVVLAFGNNLQLFLQYIWY
                     IFFIVLIHNALALSIGYGVGTAFKIPREDRRTLTIETGIQNSGLGLVLLFNPKIFPPD
                     LMNGGMLFITAWWGIWHIISGLAVSAMFNYSTKRRLARAQNINV"
BASE COUNT          511 a          431 c          459 g          492 t
ORIGIN      
        1 gcaaggggtg tttgcggtga atggcgtgaa tgctaattcg ggctttcaga cggcgctctc
       61 tgagtttatc tgccttttcc agaagctttg aagccttctt aggctcattc tccctcaact
      121 gcttgatgcg gctgataatt tccttgtttg ccttgaagcg ctccatgacc cgatacttgc
      181 atataggtcc actatggagt ttggccaact cccagactgc ttcatacttg tcatcatcgt
      241 ctatatagac tatcagctcc tgcagtcgct ctttgatctg ctgccgaatc gcctccataa
      301 tatcccgttc cgatgcacca tccttgcgca tggaggagat aaactcggat acattgaagg
      361 gttcgcctat attgcagatg gcagtagatc ggaacctgaa atagtcacca tattccactc
      421 caaccggcac aatgtatatc gggtggtcgt cacccagagc atcgtaggca ccggatgcta
      481 tcctggcaat acctttgcct atgggcaaca agctgtgcat agtgcggtgg gtaccttcag
      541 gaaggataca caaaccgaca ttgtgcttca acaccttgat ggaggccctt atggtttgct
      601 caactgccgt aaccgtcctg atgccgtccc tgatacggtt tatggggatc attttgaaga
      661 aggtgagaat tttacgaaga ataggattct tgaaaatgtc cgctctcgct ataaatacct
      721 tcctatccct gtttgcagcc agtaccagca ggggatccat cagagcgtcg caatggttgg
      781 gagtaaaaat taccgcagaa tctcttggga tccgatccgt attatggaaa atgattttct
      841 gataagagcc cttgatgacc gcatctacag aaggcctgag gatggtgtac aggggatcct
      901 tttcgtagac ttccctatgt ttccggaact tacacattga tgttctgtgc cctggcaagg
      961 cgtcttttag tggaataatt aaacattgca gagactgcca atccggagat tatgtgccaa
     1021 atgccccacc atgcggttat gaagagcatt cctccattca tcagatcggg agggaatatc
     1081 tttggattga acagcagcac cagacccaat ccggaatttt gtatgccggt ctcaatggtt
     1141 agggtacgac gatcttcacg aggtatttta aaggcagtac ctacgccata gcctatggag
     1201 agtgccagtg cattgtgaat caatactatg aagaaaatgt accagatata ctgtaaaaaa
     1261 agctgcaaat tattaccgaa cgcaagaact accatcaaaa ggaaaaagag gatggagaag
     1321 atctgcatcg gcttttttag tttttcggaa acctttggga gatacttgtt ggtcaacaaa
     1381 ccgagtatca ggggtatgcc gagcaggata aagactgtca ccataacatc cgagatggga
     1441 atctcgaggg gttgcagagc tcctgccgca tgtttattga gatacctgac ataaagccca
     1501 ccccaaagcg caaagttgcc gggagtggtg agcggggcca gcgaggtgct gatagcggtc
     1561 agagagatgg aaaactcggc attggccttg gagagctgag acatgaagtt ggaaatgtta
     1621 cctccggggc aagcagctac cagtatcata cccatggcca ccataggtgt aatacggtca
     1681 tttagtatga ttacaagtag aaatgtcagt gcaggcagga gaaccatctg gcagatagca
     1741 cccaaaatga ccgatttggg ctttttgaat acctctttga acattgaggg cttgatgccc
     1801 agtgcaacac catacatcac aaatgcgagt acgaaattta tcatatccat ccccgctttg
     1861 gagaaattaa tcttgacagg atcaagattc aat
//
LOCUS       DAOSIE010000284         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_53240_length_1880_cov_3.413699, whole genome shotgun sequence.
ACCESSION   DAOSIE010000284 DAOSIE010000000
VERSION     DAOSIE010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53240_length_1880_cov_3.413699"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1880
                     /locus_tag="PLI69_07005"
     CDS             <1..>1880
                     /locus_tag="PLI69_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOG42269.1"
                     /translation="TGALKRILVGTHGGIRSFTLTDDGSWAAYVTLLPNQVSALFLYE
                     VATGKSFQVTDKWYNSSSPVFSKDGKYLFFTSSRNFRSTYSRVEWNATYAVNDYIFVL
                     PLAKDTPNPAAVKSDEYTSAGSSTPTEKPSDKKESKSPATVKVDTDGLYERISSLPLP
                     AGGYNLLFADGGKLYYISYSGMGGSMARAGSSGVKSLDLKTMKSGDAFQGTPLSHTPD
                     LKKVLVRDAGKLFVINSAGPGKPGDPVPTSDMEIIIDRQAEWRQIFDESWRITRDGFY
                     VENMHGVDWQKIHSKYAQLLPHVKHRHDLSYIIGEMIGELNVGHAYINSGDVPELPRI
                     KMGLLGAEFAKDKSGAFKIVKIFKGANWDEALRSPLSESDAKVGEYVLAVNGIPCRDL
                     TNIYQALVGKAEKVVSLLVSPDAKGTQSRTIYVKTISDESRLAYYEWVQKNIEKVEKA
                     SNGDIGYIHIPDMGTSGLDMFTKLFYTQLDKKALIIDDRMNGGGNVSPMILERLQRVV
                     YRMSMSRNGGEPNTIPNEAHYGPKVCLIDKYSSSDGDLFPYGFKTLGLGKVIGMRSWG
                     GIVGISGSKPFIDGQDMRTPFFTSYSSETGDWIIEGYGVDPDIVVDINPFEDYLGKDA
                     QL"
BASE COUNT          507 a          418 c          454 g          501 t
ORIGIN      
        1 tacaggtgca ctcaagcgta ttcttgtcgg cacacacggc ggcattcgtt catttactct
       61 taccgatgat ggcagctggg cggcttatgt caccttgctc cccaaccaag tcagtgcttt
      121 attcctctac gaggttgcta ccggtaaatc attccaggtg acggataaat ggtacaattc
      181 atcttctccg gtattttcca aggatggaaa atatctcttc tttacctctt ccagaaactt
      241 ccgttcaacc tattcgaggg tggagtggaa tgctacctat gctgtcaatg actatatatt
      301 tgtccttcct cttgcaaagg atacccctaa tcctgctgcg gtaaaatctg acgaatacac
      361 gtctgccggg tcttctactc cgaccgaaaa gccttccgac aaaaaagaga gcaagagtcc
      421 tgcgacggta aaagttgata ctgacggtct ctatgagcgt atatcatcac tgccgctacc
      481 cgcaggcggg tacaatttac tctttgctga tggagggaaa ctctattata tttcttatag
      541 cggtatggga ggttccatgg caagagccgg ttcgtccgga gtgaagagct tggatcttaa
      601 aaccatgaaa tccggcgatg catttcaggg cacaccacta agtcatactc cggatctcaa
      661 gaaagttctc gtaagggatg caggcaaact ctttgtcata aatagtgccg gtccgggcaa
      721 gcccggtgat cctgttccca cttccgatat ggagattatc attgaccgtc aggccgagtg
      781 gagacagatc tttgatgaaa gctggcgtat tacgagagat gggttctatg tagagaatat
      841 gcacggtgtg gattggcaga agattcacag caaatatgca caactccttc ctcacgttaa
      901 acatcgccat gatctgagtt acataatcgg agaaatgata ggagagctca atgtgggcca
      961 cgcctacatc aattccggtg acgtgcctga gttgcctcgt atcaagatgg gacttctcgg
     1021 agcagagttt gccaaagaca agtccggtgc cttcaagatt gtcaaaattt tcaaaggtgc
     1081 caactgggat gaggctttgc gtagtcctct ttcagagagc gatgccaaag tgggcgaata
     1141 tgtacttgca gtaaatggca ttccctgcag agatttgaca aatatttatc aggcacttgt
     1201 cggcaaggca gagaaagtgg tatcacttct cgtctcgcct gatgccaaag gcactcaatc
     1261 ccgcacaatt tatgtaaaga ccatttcaga tgaaagcaga ttggcttatt acgaatgggt
     1321 acagaagaac atagaaaaag ttgaaaaggc ctcaaacggc gatatcggtt atatccatat
     1381 ccccgacatg ggaacctcag gtctggatat gtttaccaag ctcttttaca cccagcttga
     1441 caagaaggca ctaataatag atgaccgtat gaatggtggc ggaaatgtat cccctatgat
     1501 tttggaacgt ctacaaagag tagtttacag gatgagtatg tcccgtaatg gtggagagcc
     1561 caatactatc cccaacgagg cccattatgg cccgaaagtt tgtctgattg ataaatattc
     1621 atcatctgat ggcgaccttt tcccgtatgg attcaaaaca ctcgggcttg gaaaagtgat
     1681 aggtatgcgt tcctggggag gcattgtagg tatttcaggc tctaagccct ttatagatgg
     1741 ccaggatatg cgtacgcctt ttttcacatc ctactcctca gagacggggg attggatcat
     1801 cgagggatat ggagtggatc ccgatatagt ggtagatatc aatccttttg aggattatct
     1861 cggtaaagat gcgcagcttg
//
LOCUS       DAOSIE010000285         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_53397_length_1876_cov_3.636463, whole genome shotgun sequence.
ACCESSION   DAOSIE010000285 DAOSIE010000000
VERSION     DAOSIE010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53397_length_1876_cov_3.636463"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1540)
                     /locus_tag="PLI69_07010"
     CDS             complement(206..1540)
                     /locus_tag="PLI69_07010"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG42270.1"
                     /translation="MYLKIQRIILLLFVALTAVSCKKVTLEEQIDKIYNYFQNENTTA
                     VATLERVSNDYPARMSGSQNNDEAIDYFTYILAEEVGIKHIWHQEVEVPNWDPGVTKV
                     TMNLSNGTVVELPAIALGLNTGTSGQDITAQVVEVKSYEELETLRYKDKIVFFNGAME
                     DGFSYGKHAWQRSRGASLVTDKGAKAVLVRSISNHLGDTPHTGACHDRDSTVAVPAAA
                     ISTNAADQLSEALKMDKKLTVTINSTAHNRPEPAIGRNLVAEIPGKVNPNHIILISAH
                     VDSWFNSQGAHDNASGCVAAIEVLRAYKELRMKPRNTIRIVLYQDEEMNLSGLKEFAR
                     IAREKGESFLFDLEIDSGAGKPVGFHIFDSEDYFTKMEEVLTPYLEPRGLTLTYNDSD
                     RDWPLKTYYTLVSDREEYFKYHHSSLDNFENINPETFKESIAAITTFVFLMD"
     gene            complement(1621..>1876)
                     /locus_tag="PLI69_07015"
     CDS             complement(1621..>1876)
                     /locus_tag="PLI69_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42271.1"
                     /translation="TPGSAGTMQEIFQDAAQNHYLMDDESAPMIFFDTDYWTNRIPVY
                     KFLQDCMEIRSYANLRLSMSDDIDEVYTILEEYVKMLDSK"
BASE COUNT          471 a          438 c          426 g          541 t
ORIGIN      
        1 ttccgcctga agaaaatact tattacacgc tactttgatg acgcgtttct cttcaaaccc
       61 gatctcgcaa aacgcgctgc cgatctattt gccacctgca cggaattcaa caccattctc
      121 aaccgcgcca tcgacttcgc ctacgaagaa tattagggta gttccgagtg tgtagtgtgg
      181 agtgtgtagt accccgtacc ccgcatcaat ccatcaggaa gacaaatgta gtaatggctg
      241 caatactctc cttaaaggtc tcaggattga tgttttcaaa gttgtcgagg gagctgtgat
      301 gatacttaaa atattcttcc ctgtccgata caagtgtgta atatgtcttc agaggccagt
      361 ctctatcgct atcgttgtag gtcagcgtaa gacctcgtgg ttcaagatag ggggtaagta
      421 cctcttccat ctttgtgaaa taatcctcag aatcaaaaat gtggaaaccg acaggttttc
      481 ctgctccgga atcaatttca aggtcgaaga ggaaggattc ccctttttcc ctcgcaatac
      541 gggcgaattc cttcagccct gagaggttca tctcctcatc ttgatagaga actatacgga
      601 tggtatttcg aggcttcata cggagttctt tgtaggcacg aagcacttct attgccgcta
      661 cgcaccccga agcgttatca tgtgctccct gactgttgaa ccaactatcc acgtgagcgg
      721 aaatcaatat gatgtggttg ggattgactt taccggggat ttcagctaca agattgcgtc
      781 cgattgcagg ttcgggacga ttgtgtgccg tggagttgat agtcacggtg agctttttat
      841 ccatttttag agcctcagag agttgatcgg cagcatttgt agagatagcg gcagccggaa
      901 cggcaactgt actgtcgcga tcgtggcagg cacccgtgtg gggagtatct ccgagatgat
      961 tgctgattga gcggacaaga actgcttttg cccccttgtc ggttacaagt gaggctcctc
     1021 tggagcgctg ccaggcatgt ttaccataac tgaatccatc ctccatcgcc ccgttgaaaa
     1081 agactatttt atctttgtag cggagagttt ccagctcttc atagcttttt acttccacca
     1141 cctgagccgt aatatcctgt ccggatgtac ctgtattgag tcccagcgca atggcaggga
     1201 gctccactac ggtaccattg gagaggttca tcgtgacctt cgtcactccg gggtcccagt
     1261 tgggtacctc cacctcctga tgccaaatat gcttgatacc aacctcttct gcaagaatat
     1321 atgtaaaata atctattgcc tcgtcattgt tttgactgcc tgacattcgg gccgggtagt
     1381 cattactaac cctttcgagt gtagcgacgg cggtggtatt ttcattctga aaataattgt
     1441 aaatcttgtc gatctgctcc tcaagggtaa cttttttgca ggaaactgct gtaagagcca
     1501 caaacaagag taggatgatt ctttgaatct ttaaatacat gttcggtatt gttatataat
     1561 tgataaataa ttgttgtcct cttcggttat caaaaagcag accagagttg agcagaactc
     1621 ctattttgag tccaacatct tgacatactc ttccagaata gtgtaaactt catcgatatc
     1681 gtcagacatc gataggcgca aatttgcata actcctgatc tccatacaat cctgaaggaa
     1741 tttataaacg ggtatcctgt tggtccagta atcggtatcg aaaaagatca taggcgccga
     1801 ttcgtcatcc attaaatagt ggttctgagc cgcatcctgg aaaatctcct gcatggtacc
     1861 tgcacttccg ggagtg
//
LOCUS       DAOSIE010000286         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_53499_length_1874_cov_2.841121, whole genome shotgun sequence.
ACCESSION   DAOSIE010000286 DAOSIE010000000
VERSION     DAOSIE010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53499_length_1874_cov_2.841121"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="PLI69_07020"
     CDS             <1..1518
                     /locus_tag="PLI69_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HOG42272.1"
                     /translation="TSGNPEDFQRYAQAGIKTAEIYSDKVLFEKFSRMRTSGRVRSPI
                     LKRELDILYLSFLKGQADTTLQNLIIEKESLLEQKYAGFRAEFRGEKVNDNFIEEILR
                     TSTDNKALEEIWNAHKTIGTFVAEDVLEIVRLRNKLAQSLGFDNYHTMSLTLSEQNPE
                     EISALFDELDEMTNEGFGALKADMDKMFSARYKIPVDKLMPWHYQGRFFQEAPDLYPV
                     DFDKYYKGRNLETLTTDFYNSIGLDVNTVMMNSDLYPRDGKNQHAYCIDIDRNGDVRV
                     LCNISDTEQWMSTMLHEFGHAVYAVGHDAAGNPLFLREAAHSFTTEAVAMMFDRLSRN
                     TEWMKIMVDLPEEEAAAINEDCIRSLRLQQLVFSRWTQVMYRFEKEMYANPEQDLNTL
                     WWDLVEKYQLLKRPEGRNQPDWASKIHIALYPCYYHNYQLGALLASQLHHYIVTNVTN
                     SGDMANDCYFGNKAVGEWLQEYVFNPGMRYEWNEMIEKATGEKLTAAYYKMQFVD"
     gene            complement(1545..>1874)
                     /locus_tag="PLI69_07025"
     CDS             complement(1545..>1874)
                     /locus_tag="PLI69_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42273.1"
                     /translation="YPDSELLLGAWSSYECFKKGEEPFSYTLERYLFTEDGYVAFYDK
                     SVFQFKLPYCYNATTGILAILDGTDEQGNKLTIEYPAVIEYVEAVKSDVLTISYDDGY
                     TIRYRKR"
BASE COUNT          568 a          413 c          414 g          479 t
ORIGIN      
        1 acgagcggca atcccgaaga ttttcaaagg tatgcacagg ccggcatcaa aaccgcagaa
       61 atctattctg ataaggtctt gtttgagaaa ttcagccgaa tgcgtacaag cggcagggtc
      121 aggagtccga tactcaaaag agaattggat atactctacc tctcctttct aaagggacag
      181 gctgacacaa ctctgcaaaa tctaatcata gaaaaagaga gcctgctgga acaaaaatat
      241 gccggtttcc gtgctgaatt tcgtggagaa aaggtcaacg acaattttat tgaagagata
      301 ctacgaacat ctaccgacaa caaagctctc gaagagattt ggaatgctca caaaactata
      361 ggtacattcg tggctgaaga tgtactggag attgtgcgac ttcgtaataa gttggctcag
      421 tcactggggt tcgacaacta tcacacgatg tccttaactc tttcggagca gaatcctgag
      481 gaaatctcag cactatttga tgaacttgac gagatgacga atgagggatt tggcgccctc
      541 aaggcagata tggataaaat gttctcagcc agatacaaaa tccccgtaga caaactgatg
      601 ccctggcact atcagggaag atttttccag gaggctcccg atctctaccc cgtggatttt
      661 gacaaatatt acaaagggcg caacctcgaa actctgacta ccgatttcta caatagcata
      721 ggccttgatg tgaacactgt aatgatgaat agtgacctct atccacgtga cggtaaaaat
      781 cagcatgctt actgtattga tatagatcgt aacggagatg tacgcgtcct ctgcaacatt
      841 tcagataccg agcagtggat gtccaccatg ctacacgaat tcggtcatgc tgtttatgct
      901 gtcggacacg atgctgcagg caaccctctc ttcctacggg aagcggcgca tagtttcact
      961 acggaggccg tggcaatgat gtttgacagg ttgtcgagaa atacggaatg gatgaagatc
     1021 atggtggatc ttcccgaaga agaagctgct gccataaatg aagattgtat ccgttcgctc
     1081 cgtctccagc aacttgtctt cagtcgctgg acacaggtta tgtaccgatt cgagaaggag
     1141 atgtatgcca atcccgaaca ggatttaaat actctctggt gggatcttgt tgagaaatat
     1201 caactcctca agcgccccga aggacgcaac caacctgact gggcctcaaa aatccatatc
     1261 gcactctatc cttgctatta ccacaactac cagcttggag ccctgctggc ttcccaattg
     1321 caccattata ttgtaaccaa cgttactaat agcggggata tggctaatga ttgctatttt
     1381 ggaaataaag cggttggcga atggcttcag gaatatgtat tcaatcccgg aatgcgatac
     1441 gaatggaatg aaatgataga aaaagccacc ggagaaaaac tcacggcggc ctattataaa
     1501 atgcaatttg tggattagtc cagagttcta cactacacac taccctatcg tttgcgatag
     1561 cgtatagtat atccgtcatc gtaggaaatg gtaagaacgt ctgatttgac agcttcaaca
     1621 tattcgatta ctgccggata ttctattgtt aatttattgc cttgctcatc ggtgccatcc
     1681 agaattgcaa gaattccggt agtagcatta tagcaatagg ggagcttaaa ctggaatacg
     1741 cttttgtcat aaaaagccac ataaccatcc tctgtaaata gatatctttc aagggtataa
     1801 gaaaaaggct cttctccttt cttgaaacac tcgtacgaag accatgcacc taacaaaagt
     1861 tctgaatcag gata
//
LOCUS       DAOSIE010000287         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_54136_length_1858_cov_3.381586, whole genome shotgun sequence.
ACCESSION   DAOSIE010000287 DAOSIE010000000
VERSION     DAOSIE010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54136_length_1858_cov_3.381586"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLI69_07030"
     CDS             complement(<1..587)
                     /locus_tag="PLI69_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOG42274.1"
                     /translation="MSDRRQFLKAAGLMTAAAAFWHPRDLFAANAAKKSKVATNKLDL
                     QWENFMGKMKYTFTISGSSRTTTPIVISRITWNGYAGYGEAAMPPYLGETQASAHEFL
                     KKVAENVLPKFDNPFRIEDIMLEVDKLTTNNTAAKAAVDIALHDLVGNIMGQPWWKIW
                     GFNPDDCPNTSYTIGYDASDDMVNEKIEEASWTKV"
     gene            complement(615..1799)
                     /locus_tag="PLI69_07035"
     CDS             complement(615..1799)
                     /locus_tag="PLI69_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing C40 family peptidase"
                     /protein_id="HOG42275.1"
                     /translation="MKRLLIPAIAALMMIQSCGSPTDSFTEQFDSISKEIKAQYAPDR
                     RDKSYEPILKDVDGQKVLYGSTTEQEAKDALLAALSEKGLNVLDSMIVLPDPAFGEKK
                     WAVVSHSVANVRYEADYSSEMATQLLMGTPVQLLEKRSYWYRIVTPEGYHAWVTVGSV
                     QRMDEQELNAWKASDRLILTTYYTLLREKADATSQVLSDGVWGNIVERVGEQGQYFKI
                     KTPDGKVAFIPKTDAVTFNSWIASRKANAENIIATGKSFLGFPYLWAGTSIKAMDCSG
                     FVKTVLFLNGVITRRDASQQAKVGDDVNVENFPGDLQPGDLLFFGRKATEERGERITH
                     VGIYLGEGRFIHAATNVHTNSLMPDAEDFYDGTLVRARRIINKADEVEGLVSIAKHPW
                     YF"
BASE COUNT          433 a          452 c          409 g          564 t
ORIGIN      
        1 atcacttttg tccaggaggc ttcttctatt ttctcattta ccatatcgtc acttgcatcg
       61 tagcctatgg tgtatgaggt gttggggcag tcatcgggat taaatcccca aatcttccac
      121 caaggttgtc ccataatatt gcccacgagg tcatgcagag caatgtctac ggcagcttta
      181 gctgcggtgt tattggtggt cagtttgtcc acttccaaca ttatatcctc tatgcggaaa
      241 ggattgtcaa acttggggag tacattttca gctactttct ttagaaactc gtgtgccgaa
      301 gcctgtgtct ctcccaggta aggaggcatc gctgcctcgc catatcccgc atatccattc
      361 catgtgatac gacttatcac gataggtgta gtggtccttg atgatcctga aatggtaaaa
      421 gtgtatttca ttttgcccat gaaattctcc cactggagat cgagcttgtt cgtggcaact
      481 ttgctctttt ttgctgcatt tgcagcgaac aggtctcttg gatgccagaa tgcggctgct
      541 gcggtcatca aaccggctgc ctttaaaaat tgtcttctgt cgctcatatt tctattattt
      601 cgtagttatt atcattagaa ataccaaggg tgtttggcta ttgataccag gccttcaacc
      661 tcgtctgctt tatttataat gcgccttgca cgcaccagtg tgccgtcata gaaatcttct
      721 gcatcgggca tcagtgagtt ggtatggaca ttggtggctg catggataaa tctgccctct
      781 ccgaggtaga tcccgacatg ggttattctt tcacccctct cttcagtagc tttacggcca
      841 aagaagagca agtcgccggg ctgcaggtct ccggggaaat tctcaacatt tacatcatca
      901 cctacttttg cctgctggct ggcatccctg cgggtaataa ctccgttgag gaagagaacg
      961 gtctttacaa aaccgctaca gtccatagcc ttaatggagg tacctgccca cagatagggg
     1021 aatccgagga aactcttgcc tgtggcaatg atattttccg cattggcttt gcgagaggca
     1081 atccagctgt tgaacgtcac tgcatccgtt ttagggataa atgcaacctt tccatcagga
     1141 gtcttaattt tgaaatattg cccctgttcc ccaactctct caactatgtt tccccataca
     1201 ccgtcactta aaacctgtga ggtggcatcc gccttttcac gcaatagagt gtaatatgtt
     1261 gtaagtatca gacggtcgga ggccttccac gcgttgagtt cctgttcatc catcctctgt
     1321 acgctaccga cagtcaccca ggcatgatat ccttcgggag tgacgatcct ataccaatag
     1381 ctgcgttttt caagtaattg tacaggtgta cccatcagca gttgggtggc catttccgaa
     1441 gagtagtcag cctcatatct gacattggct accgagtgtg ataccactgc ccacttcttt
     1501 tctccaaatg caggatccgg aagcactatc atgctatcga gtacattgag acccttttcc
     1561 gagagagccg ccagcagcgc atccttggcc tcctgttccg tggtggagcc ataaaggacc
     1621 ttctgtccat caacatcttt gagtatcggt tcataactct tatccctacg gtcaggagca
     1681 tattgtgctt taatttcttt ggaaattgaa tcaaactgct cagtgaagga gtctgtgggg
     1741 gatccacagg attggatcat cattaaggct gcaatagccg gaatcaggag tcgtttcatt
     1801 cttttaaaat tattggttta aacttcaaaa atactctttt tcttatcttt gtgcaaga
//
LOCUS       DAOSIE010000288         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_55092_length_1834_cov_1.975829, whole genome shotgun sequence.
ACCESSION   DAOSIE010000288 DAOSIE010000000
VERSION     DAOSIE010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55092_length_1834_cov_1.975829"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLI69_07040"
     CDS             complement(<1..186)
                     /locus_tag="PLI69_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42276.1"
                     /translation="MGQFFTHLWLNNRSMVILIAILIVVIPFLLFYLAKAKKEHPKGL
                     IAAALSNMGERFGYYIMN"
     gene            296..1378
                     /locus_tag="PLI69_07045"
     CDS             296..1378
                     /locus_tag="PLI69_07045"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOG42277.1"
                     /translation="MKKIVITALATLFFCSSYTMAQTPKSATPDGLVIYSLPGTSINL
                     VVEAKKEIFIAGPYAQYAQKYLGTAARTENAVTYSMGSIHFHPYIEADATSRIAINLA
                     GKNIAAANFLQFCSQGLIIASDSYTGKPEAWRFPSIAANDQFAGKGVGGNLTSTTTTL
                     YKSVMTESGFQRVAVEQKQVVEKSIEKKAEEIANSIFNLRRKRVEIITGDTDAIFNGE
                     ALGAAIEEINRLEQEYLTLFFGITETSFQKMSFDVVPKAGNEKHIYVAFRISETQGLL
                     PASNMSGRPIILELILDEGTKSTTVQQAAAAAAGRSRSQMIYYRVPATVTARILDGQQ
                     VLLQTRVPIYQLGTVHSFPIETLISK"
     gene            1415..>1834
                     /locus_tag="PLI69_07050"
     CDS             1415..>1834
                     /locus_tag="PLI69_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650663.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HOG42278.1"
                     /translation="MTLSHLKPQRVWKHFYSLTQIPRPSKHEGKAVQFLEKFGKDLGL
                     ETIKDDIGNIIIRKPATPGMENRMGIILQAHIDMVPQKNNDKVHDFKKDPIQPRIVSK
                     EDGDWLYATGTTLGADNGLGCAVAMAVMEADDLVHGPV"
BASE COUNT          560 a          431 c          392 g          451 t
ORIGIN      
        1 gttcataatg taataaccaa acctttcgcc catattactc aacgcagcag ctattaagcc
       61 ctttgggtgc tcttttttag ctttggcgag gtaaaacaga aggaatggaa tgacaacaat
      121 aagaatggct ataagaataa ccatagatct gttattcagc catagatgtg tgaagaattg
      181 tcccataaat agttttttcg ttattattgg tttgtattag tttaatcaaa caatgcggca
      241 aaaatataaa aaaaatcgta tatttacttt ttgttaactc gaaaagtcaa ttcttatgaa
      301 gaaaattgtg attaccgcat tggcgaccct gtttttctgc agcagttaca ctatggcaca
      361 aacacccaaa agtgcgactc ctgacggatt agtgatctat tctcttccag gcacttcgat
      421 caatcttgta gttgaggcca aaaaagaaat attcattgca gggccctatg cgcaatatgc
      481 tcagaaatat ctcggaacgg ccgcaagaac ggagaatgca gttacttata gtatggggtc
      541 catacatttt catccctata tagaagcgga cgctaccagc cgcattgcaa ttaatctggc
      601 aggcaaaaac attgcggcag cgaacttcct gcagttctgt tcgcaggggt tgattatcgc
      661 atctgacagc tataccggta agcctgaggc ctggagattc ccctccatcg ctgccaatga
      721 ccaatttgcc ggaaaaggcg tagggggcaa tctcaccagt accactacaa ccctttacaa
      781 gagcgtgatg actgaaagcg gcttccaaag agtggccgtc gagcaaaaac aggtagtgga
      841 aaaatccatc gaaaaaaagg cagaagagat tgctaacagt atcttcaatt tacgccgcaa
      901 acgtgtggaa atcataaccg gtgacactga cgccatattc aacggcgaag ccctcggagc
      961 tgcaatcgaa gagataaacc gtctcgaaca agagtatctg actctctttt tcggtattac
     1021 cgagacctca tttcaaaaga tgagtttcga cgtagtgcct aaggccggca acgagaagca
     1081 tatctatgtc gctttccgca tctcagagac tcaaggactt ctccccgcct ccaatatgtc
     1141 cggaaggccg attatcctgg agctcatcct cgacgagggg acaaagagca ccactgtcca
     1201 gcaggctgct gccgctgctg ccggcagaag cagaagccag atgatctatt acagagtacc
     1261 cgctacggtt actgcacgta ttcttgacgg tcaacaggtg ctgctccaga ccagggtacc
     1321 catttaccaa ctcggtacgg tacactcttt ccccatagag acactaatca gtaaataatc
     1381 tatcaattta ttaaataatt aaatacaaaa cagtatgaca ctttctcatc taaaacccca
     1441 gagagtgtgg aaacacttct actccctcac tcaaattccc cgtccttcaa aacacgaagg
     1501 caaagcagtt caattccttg agaaatttgg taaggacctc ggtctcgaaa ccataaagga
     1561 tgacattggc aatatcataa tccgcaagcc ggctactcca ggtatggaaa acagaatggg
     1621 tataatcctg caggcacata tagatatggt acctcagaaa aacaatgaca aggtacatga
     1681 cttcaaaaag gaccctatcc agcctcgtat cgttagtaaa gaggatggcg actggcttta
     1741 tgctaccggc actactctcg gagccgacaa tggactcggc tgcgctgtag caatggcagt
     1801 aatggaggct gatgaccttg tgcatggacc tgtt
//
LOCUS       DAOSIE010000289         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_55162_length_1832_cov_3.123241, whole genome shotgun sequence.
ACCESSION   DAOSIE010000289 DAOSIE010000000
VERSION     DAOSIE010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55162_length_1832_cov_3.123241"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..>1832
                     /locus_tag="PLI69_07055"
     CDS             282..>1832
                     /locus_tag="PLI69_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42279.1"
                     /translation="MKRSFLYHTMLIVLSAFPLLLNAQTSKPALDHSVYDGWKSAGNV
                     TVFNDGEWGQFYLSPQEGDGTIEFYNLMTGRRISIERGQQVRFSKDATRAIFKIIPKF
                     QETRQAKIDKKKPKDMPKDTLGILNLFTGEVTKFPLLKNFKSGVLTGDYIAFQRDTVS
                     NLYILNIKTMALDSVKSVSEYDFSRENDILTYTTKPDPKKDSTTVPGIYLLNPATGVT
                     TTIIQGSKDATFGNTYFTEDGSKMAFYANTDTTKEAKKFTNIYLYDGTETRVLVPKDA
                     EWIPEGWMVSDKGSISFSKSGDYITYGTCPIPREKDTTLVDFEQPKLDIWSWNEDYIQ
                     PIQLKNLSREQRRTYLTKINIDGTNPVQLADMTLESIGIGDENKEQYVITYTDKPYRM
                     SQQWDLNPKNDIYKVSLADGSKELIAKGAPFNMLSSSPDGKYYTFYDAEARIWYLYTL
                     ATGEIRDLTSALGVVFYDEDHDTPSLPRAYSIAVWSENSDYFIIRDRYDYWQFDPTGA
                     KAPFLLTEG"
BASE COUNT          569 a          414 c          377 g          472 t
ORIGIN      
        1 cagtgccgcc aacatcaggg ttgacaagag tagaataagt gcttttctca taagttgcca
       61 aaaaacgttt tgttaatcta taaactacaa agatacttct ttttttgatt gcacaaagaa
      121 atgaagatac aaaatatggg ttgaaaagcg cgtgtgaggg taatctacga gatgttttaa
      181 agacaatgtc taagaaagag gttggtgttt ttgattatta tcgttaactt tgtaagatac
      241 tctaatagca gaaaaattta ctatttaaca ataaaaacat gatgaaacgt tcttttctct
      301 accacacgat gctgatagtg ctttcagcat ttcctctcct cctcaatgcg cagacatcca
      361 aaccggcatt ggaccattcg gtctatgacg gctggaagag tgccggcaac gttaccgtct
      421 tcaatgatgg tgaatggggt caattttatc tttcacctca ggagggcgac ggcactattg
      481 aattctacaa cctgatgaca ggcagacgga tttccattga acgaggccaa caggtcagat
      541 tttcaaaaga tgctaccaga gcgattttca aaataattcc taaatttcaa gagactcgcc
      601 aggccaaaat agataaaaag aagcccaagg atatgcccaa agatactctc ggcatcctta
      661 atctcttcac aggcgaagtg acaaaattcc cgcttcttaa gaattttaag tccggagttc
      721 tcaccggtga ttacatcgca ttccagaggg acaccgtatc taatctctat atattaaaca
      781 tcaagacgat ggccttggat tccgtgaaga gtgtctccga atatgatttc tcgagggaaa
      841 atgatatcct tacctacact accaagccgg atcccaaaaa ggattccacc accgttcccg
      901 gcatctattt gctaaatccc gcaacgggcg tgactacaac aattattcag ggttccaaag
      961 atgcgacttt cggcaatacc tattttaccg aagatggatc aaagatggca ttttatgcca
     1021 ataccgatac tacaaaagag gctaagaaat ttaccaatat ctatctctat gatggtacag
     1081 agaccagggt gctcgtcccc aaagatgcgg aatggattcc cgaaggttgg atggtcagcg
     1141 acaaagggag catcagtttc agcaagtcgg gagattatat cacatacggt acctgtccga
     1201 tcccacgcga aaaggatacg acacttgtgg attttgagca gcccaaactg gatatctgga
     1261 gctggaatga ggattatatc cagcctatcc agttgaaaaa cctcagtcgc gagcaaagac
     1321 gcacctatct gacaaaaatc aatattgacg gcacaaatcc cgtgcagttg gccgatatga
     1381 ctctcgagag catcggcatt ggagatgaga acaaagaaca atatgttatt acctacaccg
     1441 acaaacctta ccgcatgtca caacagtggg atttgaatcc aaaaaacgat atttacaaag
     1501 tctcactcgc agacggcagc aaggaactca ttgccaaggg cgcccctttt aatatgctaa
     1561 gctcttcccc agacggcaag tattacactt tttatgacgc agaagctagg atatggtatc
     1621 tctacacact tgcaaccggt gaaattagag atctaacttc agcattaggt gttgtcttct
     1681 acgacgaaga tcacgacacc ccctctctcc ctagagctta tagcattgct gtctggtcgg
     1741 aaaattcaga ctatttcatt attcgcgaca gatatgacta ctggcagttc gaccctaccg
     1801 gagcaaaagc tcctttcctc ctgactgagg gt
//
LOCUS       DAOSIE010000290         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_55251_length_1830_cov_2.841127, whole genome shotgun sequence.
ACCESSION   DAOSIE010000290 DAOSIE010000000
VERSION     DAOSIE010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55251_length_1830_cov_2.841127"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PLI69_07060"
     CDS             complement(<1..1108)
                     /locus_tag="PLI69_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOG42280.1"
                     /translation="MQSIIITGGAGFIGSHVVRHFVKKYPDYKIINVDKLTYAGNLAN
                     LEDIENVPNYRFVRADICDFDAMFALIWEEKVDGIIHLAAESHVDRSIKDPFTFARTN
                     VMGTLSLLQAARISWESLPEKYEGKRFYHISTDEVYGDLEITHSEGIESPFADMAAPS
                     GHNLAYGTDFFLESTPYAPHSPYSASKAASDHFVRAFHDTYGMPTIVTNCSNNYGPYQ
                     FPEKLIPLFINNIRKGKPLPVYGKGENVRDWLYVEDHARAIDLIFHRGKIGETYNIGG
                     FNEWKNIDLIKVIIATVDRLLGNPEGYSLDLITFVTDRAGHDLRYAIDSRKLQKELGW
                     EPSLQFEEGIEHTVRWYLENQEWMDRITSGDYERY"
     gene            complement(1122..>1830)
                     /locus_tag="PLI69_07065"
     CDS             complement(1122..>1830)
                     /locus_tag="PLI69_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG42281.1"
                     /translation="PGFKRLLGDGSDFGVRFDYAEQPSPDGIAQAFIIGDKFIGKESA
                     CLVLGDNIFHGAGFTGMLKEAVRAAEEDAKATVFGYWVADPERYGVAEFDEEGNCLSI
                     EEKPAKPKSNYAVVGLYFYPNKVVNVAKNVKPSARGEIEITSVNQRFLEDGELKVQTL
                     GRGFAWLDTGTHDSLSEASTFVEVIEKRQGLKIACLESIAFSKGWISEEKMREVAQPM
                     ARNQYGQYLLKIVENKQ"
BASE COUNT          472 a          452 c          400 g          506 t
ORIGIN      
        1 aatagcgctc atagtcgcct gaggtgatcc tgtccatcca ttcctgattc tccagatacc
       61 agcgaacggt atgttctatg ccctcttcaa attgtaatga aggctcccat cccaattctt
      121 tctgaagttt tctggaatct attgcgtaac gcaaatcgtg gcctgcacgg tccgtaacaa
      181 aggttatcag atccaaagaa tatccttcgg gattaccaag cagacggtca accgttgcta
      241 ttatgacctt tatcaggtct atgtttttcc actcattgaa accgccgata ttataggtct
      301 ctcctatctt tccacgatga aaaatcaggt caatggccct ggcatgatcc tctacgtaga
      361 gccagtcgcg gacattttca cctttaccat ataccggcag cggttttccc ttacgaatgt
      421 tgttgatgaa tagcgggatc agcttttcag ggaattggta tggcccataa ttattagagc
      481 agtttgtaac tatggtcggc ataccgtagg tatcgtggaa agcacgaacg aagtggtctg
      541 atgccgcctt ggaagcggaa tagggtgaat ggggagcata gggagtgctt tcgaggaaga
      601 aatccgtccc ataagcaaga ttatgccccg aaggagctgc catatcggca aatggtgact
      661 caatcccttc tgagtgggtg atttcaagat caccatacac ctcatcagtg gagatatggt
      721 agaagcgctt gccctcatat ttctcaggaa gtgactccca ggagatcctg gcagcctgta
      781 gtagagaaag agtccccatt acattagtgc gggcaaaagt aaaggggtct ttaatcgagc
      841 ggtctacatg cgattcagca gccagatgga tgatgccgtc aaccttctcc tcccatatta
      901 gagcaaacat agcatcaaaa tcacagatat cagcacgaac gaaccgatag ttaggtacat
      961 tttcgatatc ttcgagattg gccaaattgc ccgcataagt gagtttatcc acgttgataa
     1021 tcttgtaatc gggatatttc ttaacaaaat ggcgcactac gtgcgatccg atgaatccgg
     1081 cacctccggt tatgataatg ctttgcatat cgtttaaaaa attattgttt gttctctact
     1141 atcttgagca aatattgtcc atactggtta cgtgccatcg gctgagcaac ttcgcgcatt
     1201 ttttcttcgg aaatccagcc tttgctgaaa gcaatgctct cgaggcaggc tattttgagc
     1261 ccctgacgct tttctatgac ctctacaaaa gtggatgctt cacttaggga atcgtgcgtg
     1321 ccggtatcaa gccaggcaaa tcccctccca agagtctgta cctttagttc tccatcctcg
     1381 agaaatctct gattgactga agtaatctca atttcgccac gtgcagaggg tttgacattc
     1441 tttgctacat tcaccacttt gttgggataa aaatagagtc ctactaccgc ataattggat
     1501 tttggcttgg ctggtttttc ctcaatggag aggcagttgc cctcttcatc aaattcggcc
     1561 acaccatagc gctccggatc ggctacccaa tacccgaaga ccgtggcttt agcatcctct
     1621 tcagcggctc gcaccgcctc tttcaacatt cctgtaaagc ccgctccgtg gaatatattg
     1681 tcaccgagaa caaggcaggc actctctttg ccaataaact tatctcctat tatgaatgcc
     1741 tgcgcaatac cgtccggtga gggttgctcc gcatagtcaa aacgcactcc gaagtcactt
     1801 ccatcgccaa gaagacgttt aaatcccgga
//
LOCUS       DAOSIE010000291         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_55313_length_1828_cov_5.148336, whole genome shotgun sequence.
ACCESSION   DAOSIE010000291 DAOSIE010000000
VERSION     DAOSIE010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55313_length_1828_cov_5.148336"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..823
                     /locus_tag="PLI69_07070"
     CDS             53..823
                     /locus_tag="PLI69_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42282.1"
                     /translation="MSDIMDVAPITDVPAIADDQLLVIADQAEKRIEAVKKIKSLVFR
                     VTNFHDWIDQQGKPYLWASGAEKIARLFGISWRIDEPVFESLEGGHYSYTYKGYFTLG
                     GVTIEAVGARSSKDGFFKKYGKETNGERKELPPSEIDKTDVKKAAYTNLIGNGITRLL
                     GIRNLTWEELKEAGITKEKATTVDYGSSKEQRTGCISDAQRKRFYAIAKKAGWSDDEI
                     KDYLKTIGVESSKDIPTDKYEDACKWAETRQRQPGEDD"
     gene            823..1770
                     /locus_tag="PLI69_07075"
     CDS             823..1770
                     /locus_tag="PLI69_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HOG42283.1"
                     /translation="MIVERVIDQKAREIKQWPVRSNRASSLGHPCTRYLTFERTHWQE
                     KVLHGPSLQMVFDIGNDVEDRVLRDLREAGFTVIEQQRAFEWAKYGITGHIDAKIQIG
                     STVYPIEVKSMSPFVFDKTNTVADMLNSKYHYMRSYPAQMTLYLLMDNKEAGFFILKN
                     KSTGAMKEIEVNLDYELGEKLLKKAETINRHVEAGTLPDPIEWDDNVCSECGYLHICN
                     PVRTGTEVDIVDDEELLELLIKRESLQVYAKEFEEIDGILKEKLEGRDKLLIGDYYIT
                     GSWRKTTRYEVPKDIKDQYKTEAQYWVRKIAKVTDLRKG"
BASE COUNT          585 a          386 c          477 g          380 t
ORIGIN      
        1 gaacaggaag acaaaacgga cttcaggtcc cgcaatgata taaggaggga gtatgagtga
       61 tataatggat gtagcaccaa taacggatgt accagcaata gcagacgatc agttattagt
      121 tattgctgac caggctgaga agcggatcga ggcggtaaag aagatcaaat ccttagtatt
      181 cagggtaact aacttccatg attggattga ccaacagggc aagccgtacc tgtgggcatc
      241 aggcgccgaa aagattgccc ggctgtttgg tatctcttgg agaatagacg agcccgtctt
      301 tgaatcgtta gagggaggcc attacagcta cacatacaaa ggctatttca ccctcggtgg
      361 cgtcacaatc gaagcggtag gcgccagatc ctcaaaggac ggtttcttca agaagtacgg
      421 caaggaaacc aatggagaaa gaaaagagtt gccaccttca gagattgata agaccgatgt
      481 caagaaggct gcatacacga accttatcgg taatggcatc actcggcttc tcggtatccg
      541 caatctcacc tgggaagaac tgaaagaggc cgggataact aaagagaaag ccaccacggt
      601 tgactacggt tcaagcaagg aacaaaggac aggatgtatt tctgatgctc aaaggaaacg
      661 cttttacgcc atagcaaaga aagcaggatg gtctgacgat gaaattaagg actacctcaa
      721 gacaataggc gtggaatcct cgaaagacat tcctacggac aaatatgaag atgcttgcaa
      781 gtgggcagag acgagacaga gacagccagg ggaggacgac taatgatagt agagcgagtg
      841 atagaccaga aggcaaggga gataaaacaa tggcccgtga ggagcaaccg ggcctcttcc
      901 ctgggtcatc cctgcacacg gtatcttacc tttgagcgca cccactggca ggagaaggtc
      961 ttacacgggc cttctctcca gatggtgttt gatatcggta acgatgtgga agatagggta
     1021 ttgcgggatc ttagagaagc aggattcaca gtaatcgaac agcaacgggc tttcgagtgg
     1081 gccaagtacg gaattactgg ccacatcgac gccaagatac agataggttc taccgtatac
     1141 cctatcgagg ttaagagtat gagcccgttt gttttcgaca agaccaacac ggtagctgat
     1201 atgctcaatt cgaaatacca ctacatgaga tcttaccccg cacagatgac attgtacttg
     1261 ctgatggaca ataaagaggc aggatttttt attctcaaaa acaaatccac cggcgccatg
     1321 aaagagatcg aggtaaacct tgactacgag ctcggagaaa agttgcttaa gaaagcagaa
     1381 actatcaacc gtcatgtcga ggctgggaca cttccggatc ccatcgaatg ggacgacaac
     1441 gtatgctcgg agtgtggata cctccatata tgcaacccgg taaggaccgg aacagaagtt
     1501 gacattgttg atgatgagga gctcctcgaa ttgctcatca agcgggaatc attacaggtg
     1561 tatgcaaaag agtttgagga gatagacggg atcctcaaag agaaacttga gggccgtgac
     1621 aaactattaa ttggtgacta ttacatcacg ggatcctggc gtaagacaac caggtacgaa
     1681 gtcccaaaag atattaagga ccagtataag accgaggcgc aatactgggt gcgtaaaata
     1741 gcaaaagtga cagatctcag aaaaggatag ctgcgtaata atgttgacta actcccctaa
     1801 caagtctaac cttttccaaa cgataggg
//
LOCUS       DAOSIE010000292         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_55389_length_1827_cov_2.422122, whole genome shotgun sequence.
ACCESSION   DAOSIE010000292 DAOSIE010000000
VERSION     DAOSIE010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55389_length_1827_cov_2.422122"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLI69_07080"
     CDS             <1..387
                     /locus_tag="PLI69_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOG42284.1"
                     /translation="SVKSAVAKEADVVIIDTAGRLHNKINLMNELTKIRNVMSKVVPD
                     APHEVLLIIDGSTGQNGFIQAREFVKATEVTALAVTKLDGTAKGGVVIGISDQFRIPI
                     KFIGVGEKIEDLKLFDKKEFVGSLFK"
     gene            406..>1827
                     /gene="pheT"
                     /locus_tag="PLI69_07085"
     CDS             406..>1827
                     /gene="pheT"
                     /locus_tag="PLI69_07085"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOG42285.1"
                     /translation="MAVSYNWLKDYLKFDLKPSEVEKILTSTGLEVEQMKHVEQVPGG
                     LEGVIIAQVVECVEHPDSDHLHVTKVNTGAPDLLQVVCGAPNVATGQKVLLATVGSTL
                     PGEDGSGFKIKKSKIRGVESHGMICAEDELGIGTDHEGIMVLEPEAVVGTSAKDYLKL
                     KTDTIFEIGLTPNRIDGASHIGVARDLYAYCKLRDIPVEFVLPSVEGFTNVEGKAIPI
                     RVEAPDLAPRYIGITIRGVKVGPSPEWLKERLMMIGQRPINNIVDITNFLLNENGHPL
                     HVFDASKIEGGEVVVRRSASNCKFVTLDGVERTLSSEDLVICNAKDPMCIAGVFGGED
                     SGVTESTTDIFLECAYFNPTTIRKTSKRHGLKTDASFRYERGVDPLNTMYAAKRAAML
                     ITEVAGGVIVGDIQESYPNKFREVQLELDYSRMESLIGKRIESQTIKDILASLDFNIR
                     KESATGCLVDVPSYRVDVTRECDV"
BASE COUNT          519 a          368 c          477 g          463 t
ORIGIN      
        1 agcgtcaagt ctgccgtggc caaggaagca gatgtggtca taatagatac ggcaggtcgt
       61 ctgcacaaca aaatcaacct tatgaacgag cttaccaaaa ttcgcaacgt catgagtaag
      121 gttgtaccgg atgctcccca cgaagtgttg ctgataatag acggttcaac cggtcaaaac
      181 ggatttatcc aggcaaggga atttgtcaag gcaacagaag tgaccgcact ggcagttaca
      241 aaacttgatg gtaccgccaa aggtggtgtg gtcattggta taagcgacca gtttcgtatc
      301 cccataaaat ttataggggt gggcgagaag atcgaggatc tcaaactatt cgacaaaaag
      361 gagttcgtgg gatccctttt caaatagaga aaaaagatat tatatatggc agtttcctat
      421 aattggctta aagattatct caaatttgat ctaaagcctt cagaagttga aaagatattg
      481 acctctaccg gtcttgaggt ggaacagatg aagcatgtgg aacaggttcc gggaggactg
      541 gagggagtaa tcatcgctca ggtagttgaa tgtgtggagc atcccgactc agaccacctg
      601 catgtgacaa aagtaaatac cggtgcccct gatcttttac aggtggtttg tggcgcccca
      661 aatgttgcga ccggacagaa ggtcctcctt gcaactgtag gtagtacgtt gcctggcgag
      721 gatggcagtg gttttaagat caaaaaatcg aaaatccgtg gagtagagag ccatggaatg
      781 atctgtgccg aagatgaact tggcatcggt acagaccatg agggtattat ggtgttggaa
      841 cccgaagccg tcgtaggcac ttctgctaag gattacctca aactcaagac agataccatc
      901 ttcgagatcg gtcttacacc caatcgtatc gacggtgcat ctcatattgg agtagctcgt
      961 gacctctatg cctattgcaa gctgagggat atacctgtag agtttgtgct gccttcggta
     1021 gagggcttca ctaatgttga gggaaaggcc attcccataa gggtcgaagc acctgatctg
     1081 gctccccgct atattggaat cactataagg ggtgtaaagg taggcccatc tccggagtgg
     1141 ctcaaagagc gtcttatgat gatcggccaa cgtcctatca ataatattgt agatataacc
     1201 aattttcttc tcaatgagaa tggacaccct ttgcacgtat ttgatgcatc taaaatagag
     1261 ggtggtgagg ttgtagtgcg ccgtagtgcg tcaaactgca aatttgtgac ccttgacgga
     1321 gtggagagaa ccctttcatc ggaagacctg gttatctgta atgcaaagga tcctatgtgc
     1381 atagccggag tatttggagg agaagactcc ggtgtgacgg aatctacgac agatatattt
     1441 cttgagtgtg cttatttcaa ccctacaacc attcgcaaga cttccaagag acatggtctt
     1501 aagaccgatg cctcattcag gtatgagcga ggagttgatc cgcttaatac aatgtatgcc
     1561 gcaaaacgcg ccgccatgct cataacagag gttgcggggg gagtgatagt tggagatata
     1621 caggagtcct atcccaataa attccgggaa gtgcagcttg aactcgatta ttcgcgtatg
     1681 gaatccctta tcggtaaaag aatagagagt cagactataa aggatatcct ggcatcgctc
     1741 gattttaata tcagaaaaga gagtgcaacc ggttgtttgg tggatgtccc ttcatatagg
     1801 gtggatgtca cacgagagtg tgatgtg
//
LOCUS       DAOSIE010000293         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_55433_length_1826_cov_3.017504, whole genome shotgun sequence.
ACCESSION   DAOSIE010000293 DAOSIE010000000
VERSION     DAOSIE010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55433_length_1826_cov_3.017504"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /gene="fusA"
                     /locus_tag="PLI69_07090"
     CDS             <1..1630
                     /gene="fusA"
                     /locus_tag="PLI69_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG42286.1"
                     /translation="SLPIGAEENFEGVVDLISKKAYFYNLSKDLVNYEIREVPEKMVA
                     EVEEWRGKLIESVAEYNEEILEKFFEDPDSITEEEIVDALRKATIDMAVVPTTCGSSF
                     KNKGVQLLLDAVMRYLPSPLDKGEVRGIDPRNGEVLVRKSNTEEPFCGLVFKIATDPF
                     VGRLAFIRAYSGKLDAGSYVLNTRSGKKERVARLYQMHSNKQNPIECVEAGDICAAVG
                     FKELRTGDTICREDKPIILESMTFPDPVIGLAVEPKTQKDLDKLGIALGKLSEEDPTF
                     TVQTDIETGQTIISGMGELHLDIIVDRLRREFGVEINQGAPQVNYKEAITGTYHHREV
                     FKKQTGGRGKFADIIVEISPADEGVTGLQFIDEVKGGNIPREFIPSVEKGFKSAMANG
                     VLAGYEVTSMKVKLLDGSFHSVDSDSLSFEICARQAFRKALPKCSPVLMEPIMSLEVV
                     TPEEYMGDIVGDINRRRGQIVDMDSKGNARIVKAKVPLSEQFGYVTVLRTLSSGRATA
                     SMEFSHYAEVPDNIAKDVVEKHGGRRYFDNDDEE"
     gene            1648..>1826
                     /gene="rpsJ"
                     /locus_tag="PLI69_07095"
     CDS             1648..>1826
                     /gene="rpsJ"
                     /locus_tag="PLI69_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOG42287.1"
                     /translation="MSQKIRIKLKSYDHALVDKSVDRIVKTVKATGAVVSGPIPLPTD
                     KKIFTVNRSTFVNKKA"
BASE COUNT          498 a          386 c          461 g          481 t
ORIGIN      
        1 ttcgctccct atcggagctg aggaaaactt tgagggagtt gtcgacctta tctctaaaaa
       61 ggcctatttc tataatctta gtaaagacct tgtgaattat gagataagag aggttcccga
      121 gaaaatggtt gcagaggtgg aggagtggcg aggcaaactg atcgaatcgg ttgccgagta
      181 caatgaggag atacttgaga agtttttcga agatcctgat tcaatcaccg aggaggagat
      241 tgtcgatgcc cttagaaagg ctacaatcga tatggcagtg gttcctacca cttgcggttc
      301 ttccttcaaa aataaaggag tacaactact tcttgatgcc gtgatgcgtt atctgccctc
      361 tcctctggat aagggtgaag tcaggggcat agacccgcgc aacggcgagg tgcttgtacg
      421 caagtccaac accgaggagc ctttctgtgg actggtgttc aagattgcca ctgatccttt
      481 tgtaggtaga ttagccttta tcagagccta ttccggtaaa ttggatgcag gctcctatgt
      541 actcaatacc cgttccggta aaaaggaacg tgttgcaagg ctttaccaaa tgcactccaa
      601 caagcagaac cctatagaat gtgttgaggc cggcgatatc tgtgctgccg taggattcaa
      661 ggagctgcgt acgggtgata ccatttgtcg tgaagacaaa ccgattatac tcgaatccat
      721 gacatttccg gatccggtta taggtctggc cgttgagcct aagacccaaa aagatctcga
      781 caagctcggt attgctcttg gaaagctttc agaagaagac cctaccttta ccgttcagac
      841 tgacattgag accggccaaa ccattatcag tggtatgggc gagctccatc ttgatatcat
      901 tgtggatcgt cttcgtcgtg aattcggcgt tgagatcaac cagggtgcgc ctcaagtcaa
      961 ttataaagag gctatcaccg gaacatatca ccacagagag gtattcaaga agcagacagg
     1021 tggtcgaggt aaatttgccg atataattgt cgaaatctcc cctgcagatg agggtgtgac
     1081 aggacttcag tttatcgatg aagtaaaggg cggaaatatt ccccgtgaat ttatcccctc
     1141 tgtcgagaaa ggcttcaagt ctgcaatggc aaatggtgtt ttggcgggtt atgaggtgac
     1201 atccatgaag gtgaaacttc tggatggctc tttccactct gtcgattccg actcactttc
     1261 atttgagatc tgtgcccgtc aggcattccg taaggcgctt ccaaagtgct ctccggtgct
     1321 gatggaaccg attatgtctc tggaggttgt tactcctgag gagtatatgg gtgatattgt
     1381 tggtgacatc aaccgccgca ggggccagat tgttgatatg gactcaaaag gcaatgcaag
     1441 aattgtcaaa gccaaggttc ccctttccga gcaattcggt tatgtcactg ttctgagaac
     1501 actctcgtcc ggacgcgcta cagcctcgat ggaattttcg cattatgcgg aagttccgga
     1561 caatattgca aaagatgttg tcgagaagca tggcggacgc aggtattttg acaatgatga
     1621 tgaagaataa ttaaataaat tccaataatg agccaaaaaa tcagaataaa attgaaatct
     1681 tacgatcatg cgctggttga taaatcagtt gataggatcg tgaagacggt aaaggctaca
     1741 ggtgctgttg tcagtggtcc tattccgctc ccgacagaca agaagatctt cactgtcaac
     1801 cgctcgactt ttgtaaataa aaaggc
//
LOCUS       DAOSIE010000294         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_55575_length_1822_cov_3.288625, whole genome shotgun sequence.
ACCESSION   DAOSIE010000294 DAOSIE010000000
VERSION     DAOSIE010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55575_length_1822_cov_3.288625"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /gene="tilS"
                     /locus_tag="PLI69_07100"
     CDS             complement(<1..942)
                     /gene="tilS"
                     /locus_tag="PLI69_07100"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOG42288.1"
                     /translation="MEIKNKWILQELFDIELRSLTAGVENPSILVGVSGGMDSMCMAH
                     LFLHTTVPCTFRIAHVNFSLRGEESDHDEDAVCEWCKTEGIPFYTTKVDALQFAQEQS
                     ISTQMAARNLRYGWFFSLMEEHSMDFLAISHNLNDSVETLMINLLRGTGLRGLSGIRR
                     SNGPIIRPLLSVTREDISNYVALHNIPFREDSTNKESHYHRNRIRNEVFPHFEIINPS
                     FLQTIGTSMERFSEISDLLEELYKSREGTLYRMEGGVLQIDIKALKSEKSINWWLFTI
                     LDGYGFNDAQLVQIAEALKGQSGKTFFSTSHILIRDRQ"
     gene            complement(926..>1822)
                     /locus_tag="PLI69_07105"
     CDS             complement(926..>1822)
                     /locus_tag="PLI69_07105"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOG42289.1"
                     /translation="FAQDVAPADTIAAPADTVVAPKPQYWATTLTTQIGFSQLSLTNW
                     ATGGYGSISLNTFAGANANYKREKLIWDNQLQVGYGFIQSFEDGYKKSDDRLILDSKV
                     GYKAVETLYISALFNLRTQFAQGHEKGNLVSDFFTPAYVSLGFGVDYKPFRNFSINIS
                     PLTGKTVMVKNPELRAKYGNREDQFCRFELGAQIKVDGTIDVENFKVVSSLTLFSDYL
                     NKPQNVKVFWDVNADAKITRFFSVTLRTNLIYDDAIKFLDKKDKQGNLILDDNGKPIK
                     VPGVQFKELFSIGLSYTFGNKK"
BASE COUNT          477 a          424 c          411 g          510 t
ORIGIN      
        1 ttgcctgtcc cggataagga tgtgagaagt ggagaaaaaa gtcttgccgc tctgtccctt
       61 taacgcctct gctatctgca ccagctgggc atcattgaat ccatagccgt ccagtatcgt
      121 aaagagccac caattgatgc ttttttccga cttaagtgct ttgatgtcaa tttgcagcac
      181 tcccccttcc atacggtata gagtgccttc tctggattta tagagttcct ccagcagatc
      241 cgaaatctcg gaaaacctct ccatagatgt gccgatagtc tgcaagaagg agggattgat
      301 tatctcaaaa tggggaaaaa cctcattgcg aatccgatta cgatggtaat gcgattcttt
      361 gtttgtggaa tcctcccgaa atggaatgtt gtgcagagcc acatagttgc tgatatcctc
      421 ccttgtgacg gaaagcagtg gacggattat gggaccgttt gagcgtctga tacccgaaag
      481 tcctcgcagt cccgtccctc tgaggagatt gatcatcaaa gtctctacac tgtcgttcaa
      541 attgtgagaa atagcgagga agtccattga gtgctcttcc atcagggaga aaaaccaacc
      601 gtatcttagg ttgcgtgccg ccatctgagt ggagatcgat tgctcctgtg caaattgtag
      661 agcatcaacc tttgtcgtgt agaagggtat tccctctgtt ttacaccact cgcatacggc
      721 atcctcgtcg tgatcgctct cctcccctct aagcgagaaa ttaacgtgtg cgatcctgaa
      781 tgtacagggc acggtcgtgt ggagaaaaag atgagccata cacatagaat ccattccacc
      841 gctgactcca acgaggattg atgggttttc aacgccggct gtaagggagc gcaattctat
      901 gtcaaaaagc tcctgcaaaa tccacttatt ttttatttcc aaaagtgtac gacaatccta
      961 tgctaaaaag ttccttgaac tgtacccccg gtactttgat cggttttcca ttatcatcaa
     1021 gaataagatt gccctgcttg tcttttttgt cgagaaactt tattgcatca tcatagataa
     1081 ggttggtcct aagagttacg gagaagaatc tggttatttt cgcatccgca tttacatccc
     1141 agaagacctt gacgttctga ggcttattca gatagtccga gaagagtgtg agtgaactga
     1201 ccaccttgaa attttcaaca tctatcgtgc catccacctt gatttgggca ccgagttcaa
     1261 atctgcaaaa ctgatcttcc ctgttaccat attttgcccg cagttcggga ttctttacca
     1321 taacggtttt tcccgtaagg ggcgaaatat tgattgaaaa gttacggaat ggcttatagt
     1381 ccacaccaaa accaagggac acatatgccg gagtgaagaa atccgatacc agattgccct
     1441 tctcatggcc ctgagcaaac tgggttctaa gattgaacag ggctgatatg tatagggttt
     1501 ctactgcctt gtaacccact ttgctatcca atatcaaacg gtcgtcactc ttcttgtaac
     1561 catcttcaaa tgattgtatg aagccatagc ccacctggag ttggttgtcc caaataagtt
     1621 tttcccgctt atagttggca tttgcaccag caaacgtatt gagtgaaatg gagccgtaac
     1681 ctccggttgc ccagttagta agtgaaagct gtgaaaaacc gatctgcgta gtaagagttg
     1741 tggcccagta ttggggcttg ggagccacga ccgtatctgc cggagcagca attgtatcgg
     1801 caggagctac atcttgtgca aa
//
LOCUS       DAOSIE010000295         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_55674_length_1820_cov_2.732578, whole genome shotgun sequence.
ACCESSION   DAOSIE010000295 DAOSIE010000000
VERSION     DAOSIE010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55674_length_1820_cov_2.732578"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLI69_07110"
     CDS             <1..1002
                     /locus_tag="PLI69_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOG42290.1"
                     /translation="VFTPKGESKNLPKGSTALDFAYVIHSEVGNRAIAAKVNQKLLPL
                     SHELSNGDQVEIITSDSGKPKREWLDFIRTNRARNFILDSIKAETKDSIKEGMLFLTS
                     KLKERNITIKSRVIKKLIDYYKAGSKDELYHKIGIGLIDLSDLETALKTNVEKRNVQM
                     WGVKLLTPERNSGEIDKSQDYELREDLSKGTTTFTIADCCSPIPGDNVVGFIEDDGSV
                     LVHKKSCQRATDIAAKQADRIVSAKWSKHFMMSYLARISIRGLDRMGIVSDMSKVISL
                     QLGVNMRKICIETHDEIFEGTVDLYVRNTDDLDKLIKSLSAIKGIESVKRVELNSNR"
     gene            1016..>1820
                     /locus_tag="PLI69_07115"
     CDS             1016..>1820
                     /locus_tag="PLI69_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG42291.1"
                     /translation="MQNNGRVWMTALALTGFIMCLLLSKSCANTTTPPSGGPKDTIPP
                     VLLKINPEFNATNFPRYDGTISLTYDEYTVVKNVAEIYLSPPIKKRITHKIKGKNIVF
                     SIPDTLAENRTYTLDFGEGLADNNEGNIAPRLVYAFSTGDTVDSLYITGNIIDCQTLQ
                     PVKKVLVALYTDHSDSACFKIFPDAASKSDEWGFFSIRNISDTLYRIVAFTDSDNDNR
                     YDPTKDQIAFLDTLIRPVEVVRDSIYELKSFDMKDTLACMERKSMFELLM"
BASE COUNT          577 a          410 c          401 g          432 t
ORIGIN      
        1 gtatttacac ctaaaggtga atccaagaat ctgcccaagg gctctacggc actcgatttc
       61 gcttatgtaa tacactcgga ggtaggcaat agagctattg cggcaaaggt aaaccaaaaa
      121 ttattgcctc tttcgcatga gctctccaat ggagatcagg tggagatcat tacctcggat
      181 agcggcaaac ccaagaggga gtggctggac tttatccgca ccaacagggc gcgaaatttt
      241 atccttgact ctattaaagc tgaaaccaaa gacagtatca aggagggaat gttgttcctc
      301 acatccaaac tgaaggagcg taatatcacc ataaagagcc gtgtaatcaa gaaactcata
      361 gattattata aggcggggag caaggacgag ctctaccaca agataggtat cggattgatt
      421 gatctttcag accttgaaac ggcactcaaa accaatgtag agaagcgtaa tgtacaaatg
      481 tggggagtga aactcctgac acccgaacga aatagcggcg aaattgataa aagtcaagac
      541 tacgaactaa gggaggacct atccaaaggc actacaacat ttaccattgc cgattgctgc
      601 tctcctatcc ccggagataa tgtagttggc tttatagaag atgacggaag cgtacttgtg
      661 cataagaaga gttgccagag agctaccgac attgcagcaa aacaggctga caggatagta
      721 tcggcaaagt ggagcaaaca ctttatgatg tcatacctgg cacggatttc cattagaggt
      781 ctagatagga tgggcattgt gagtgatatg tcaaaagtca tctcccttca gctcggtgta
      841 aatatgcgta aaatatgtat cgaaactcat gacgaaatat tcgaagggac tgtagatctc
      901 tatgtgcgca atactgatga ccttgacaaa ctcatcaagt cactctctgc cataaaggga
      961 atagagagcg ttaaacgtgt ggaactcaac tcaaacagat agtaaaacga caccaatgca
     1021 aaataatgga agagtatgga tgactgcgct ggctttaacc ggctttataa tgtgcctgtt
     1081 gctttccaaa tcatgcgcca atactaccac gccaccaagc ggaggtccga aagataccat
     1141 accacccgta ctcctaaaga taaatcccga attcaatgcc accaactttc ccaggtatga
     1201 tggaacaatc tcccttactt atgatgaata tacggtggta aagaatgtgg cggagatcta
     1261 cctctctccg cctataaaaa agagaataac ccacaagata aagggtaaga acatcgtttt
     1321 ttccataccc gatacgcttg ccgaaaacag gacctacact cttgacttcg gagaggggct
     1381 ggccgacaac aacgagggca acatagcccc gaggcttgtt tatgcttttt cgacaggtga
     1441 tacggtagat tctctctata taaccggcaa cattatcgac tgccagactc tccagccggt
     1501 caagaaggtg ctagtagcac tctataccga ccactccgac tcagcctgtt tcaaaatatt
     1561 tcccgatgct gcctcaaaga gcgacgaatg gggctttttc tccataagaa atataagtga
     1621 tacactttat cgtatagttg ccttcacaga cagcgacaat gacaacagat atgatcctac
     1681 aaaagatcag attgcatttc tagacacgct tatccgaccc gtagaggtag ttcgagactc
     1741 catatacgaa ttgaagtcct ttgacatgaa agacaccctc gcctgcatgg agcgtaagtc
     1801 aatgtttgag ctgcttatgt
//
LOCUS       DAOSIE010000296         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_55720_length_1819_cov_2.060658, whole genome shotgun sequence.
ACCESSION   DAOSIE010000296 DAOSIE010000000
VERSION     DAOSIE010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55720_length_1819_cov_2.060658"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1819)
                     /locus_tag="PLI69_07120"
                     /pseudo
     CDS             complement(1..1819)
                     /locus_tag="PLI69_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696269.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
BASE COUNT          426 a          501 c          397 g          495 t
ORIGIN      
        1 caccgcgatc gccaccaccg ccgtgcccgc cgtgaggacg gggacctgca ggacgaggcc
       61 tgcgtacagg ctccacataa ccttcgggtt tctccaaaag ggctctgcgg gaaagacgga
      121 gtttgttggt tttgggatcg atctcgataa gtttgacctc aacctcgtca ccaagtttga
      181 agagatcctc caccttctca gtcttgcccc aatccatctc tgaaatatgc aacaaaccat
      241 ctttaccggg gagaatctcc acaaacgcac caaactccac aactgacacc actttacctt
      301 tgtaaatttc acccacttcg gggactgcta ttatggcttt gatgcgatct aaggcagcat
      361 ccattttctc cttatcaact ccaaaaatat cgaccagacc gataggttca cccttatcat
      421 tctttgactc tgtaatggtg atacttgtat cggtttgctt ctggatatcc tgaataacct
      481 caccgccctt gccgataatt gcacctatga actcaccggg gaccacaatc tgaatgatgc
      541 gcggcacatg gggtttatat tctgcacggg gctcggagat agttttgagc atttcattca
      601 aaatatgatg gcggccctga cgtgcctgct caagtgcttt ttccaacact tcgtatgaga
      661 gaccatcaac tttgatgtcc atttgagtgg ccgtaatacc atctttcgtc ccggccacct
      721 tgaaatccat atctccgagg tggtcctcat caccaagaat atctgaaagg atagcatatt
      781 tgtcggactc ggtatctgaa atcagaccca ttgctatacc tgaaaccggc ttggaaatct
      841 tcacgccgca atccatgagt gcaagagaac ccgcacatac ggtagccatc gaagaggaac
      901 cgttggattc gaggatatca gatactatac gcactgcata gggattctct tcacctaccg
      961 gcacaacctc tttcagtgct ctccaggcga gattgccatg acctacctct cggcggctca
     1021 caccacggga tgcccttgct tcaccggtag agaaaggagg gaaattatag tggagcacaa
     1081 actgatcggt accttgagta agcacctcgt ctctctcctt catatcgtcc tttgtgccga
     1141 gggttaccgt agtaagcgat tgtgtctcac ctctggtgaa aatagctgag ccgtgagcac
     1201 cggggagata atccacctca caccagatag gacggacctc atcgctcttg cgaccgtcga
     1261 gacggatgcc ctcgtcgagg atcatacggc gcatagcctt tttctcaatg gcattgaagt
     1321 atcttgaaac cattgttgct ttttcttctc tctcctcttc agggatagtc tcaagaaact
     1381 cctggtgtag cgcttcaaac tgatcggtac gggtatgttt gtcgctgccg ctcttggcga
     1441 ttgcgtaaca tctgtcatag caatattccc aggcagcttt ctcaaccgcc tcgtcatctg
     1501 tctcgtggca atattcgcgt ttgatctctt tacccacctc tttctccagt tccttctgca
     1561 ctaagcaatg ctttttgatc tccgtatggg caaatttgat agcttcaagc atatcggcct
     1621 cgctaacctc tttcatttca ccttccacca tcattatgtt ttcgtaagtg gcggccacca
     1681 ttatgtccat atcagctttt tccaactgct cgaaagtggg gttgatgcag aattttccat
     1741 ctatacgtgc aacgcgtacc tcagagatgg gccatcgaag ggtatgtcgc tcactgcgag
     1801 ggcggctgaa gctgcaagt
//
LOCUS       DAOSIE010000297         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_55832_length_1816_cov_4.303237, whole genome shotgun sequence.
ACCESSION   DAOSIE010000297 DAOSIE010000000
VERSION     DAOSIE010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55832_length_1816_cov_4.303237"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1816)
                     /locus_tag="PLI69_07125"
     CDS             complement(<1..>1816)
                     /locus_tag="PLI69_07125"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG42292.1"
                     /translation="EEKVLLDRIEELFEIYRVTTDEEERTAIYKEIDSISYEASKIAI
                     PNEYDKLMASIGARGTNAYTGNDVTCYVENIPSNQIDNWARIQADRFANSVIRGFHTE
                     LETIYEEYNMYAAMDSEKQFEKLFAELFKKHTYGTPVIGWANHLKNPSITNVKNYKAE
                     WYVPNNMAVCLAGDFDPDKAIKIIDKHFSALKPNPNLPKLEFEPEDPITEPRKFEVLG
                     VESPSTIIAWRFPGAASPEALMLELMRSVLYNGSAGLFDLNVNQQQKTLGIGAGNYSL
                     ADYSVFIIQGEPKKGQTLDEVAAIAFEELEKIKSGNFDDDLLPSIINNMKRQKMQQNQ
                     NASYIVRSLVNCFIDGIAWADYISEVDRLSKITKEEVVAFANEHFADNYVRVDKIEKK
                     DPTDTKIAKPAITPIFTNRDTSSAFLREIQASQVAPIEPVFVDFEKDMDILTAKSNIQ
                     VLYKQNTINDLFTLTYLFDTGSYADKVLPIAADYIGYLGTSTMSKEEFQKALYKIACS
                     VSVSCTGERTYVTISGLGENMEEAVKMMEELVADAQPDEEVLTLLKGNMLQERANAKL
                     GQRDIFSRLQQYGIYGPENPSTNVLSDKTNEQVCPNWEH"
BASE COUNT          422 a          403 c          447 g          544 t
ORIGIN      
        1 gtgctcccaa tttgggcaaa cctgctcatt tgttttatcc gagagaacat ttgtggaggg
       61 attttcaggt ccgtagatac cgtattgctg gagacgcgag aagatgtccc tctgtccgag
      121 cttcgcatta gcacgctcct gaagcatatt gcccttgagc agagtgagaa cctcctcatc
      181 gggttgtgca tcagccacca gttcttccat cattttaact gcctcttcca tattttcccc
      241 gaggccggaa atggttacgt atgtccgttc accggtgcaa cttacactaa ccgaacaagc
      301 gatcttgtac aatgccttct gaaactcttc cttactcatt gtacttgtac ccagataacc
      361 gatatagtca gctgcgattg gaagcacttt gtcggcatag ctgcccgtgt cgaagagata
      421 tgtgagtgta aataggtcat tgatggtgtt ctgcttgtag agtacttgta tattgctctt
      481 tgcggtcaat atatccatat ccttttcaaa gtctacaaat acgggttcaa tgggtgccac
      541 ctgtgaagcc tgaatctcac gcaagaatgc gctggaagta tctctattgg taaaaatagg
      601 ggtgatggca ggctttgcta tcttggtgtc cgtagggtct tttttctcaa ttttgtccac
      661 gcgaacgtag ttgtctgcga aatgctcatt ggcgaatgct accacttcct ctttggtaat
      721 cttggagagt ctgtcaacct cgctgatata atcagcccac gcgatcccat cgataaagca
      781 gttaaccaga gagcgcacaa tgtatgatgc gttctgattc tgctgcatct tctggcgttt
      841 catgttgttg atgatggagg gcaggaggtc atcatcaaag ttgccggatt tgattttttc
      901 aagttcttcg aatgcgattg cagccacttc gtcaagagtc tgtccctttt tgggctcccc
      961 ctggattata aatacagagt agtctgcgag agagtagttg cctgcaccaa taccgagagt
     1021 cttttgctgt tggttgacgt tgaggtcaaa aagacctgcc gatccgttat aaaggactga
     1081 acgcataagt tcgagcataa gggcttcggg agatgcagcg ccggggaatc tccaggcaat
     1141 tattgtactg ggagactcaa ctccgagaac ctcaaacttg cgaggttcgg tgatgggatc
     1201 ttcgggctcg aattcaagct tgggaagatt ggggttaggt ttgagagccg agaaatgttt
     1261 gtcgatgatt tttatagcct tatcggggtc gaaatcaccg gcaaggcaga ctgccatatt
     1321 attggggaca taccactctg ctttgtaatt tttcacattt gtaatggaag gattcttgag
     1381 atgattagcc cagccgataa ccggagtacc ataagtatgt ttcttgaata gttcggcaaa
     1441 gagtttttca aactgctttt cgctatccat cgcagcgtac atgttgtact cctcgtagat
     1501 ggtctccaac tcggtgtgga atccgcggat cacgctgttg gcaaacctgt cggcctggat
     1561 tctggcccag ttgtcaatct ggttggaggg gatattctcc acatagcatg taacatcgtt
     1621 gccggtatat gcgtttgtgc cgcgggctcc gatgctggcc atcagtttgt catactcatt
     1681 gggaatggca atcttcgaag cttcatagga gatggaatcg atctctttat agatagctgt
     1741 acgctcctct tcatcagtgg taacacgata aatttcgaag agttcttcta tgcggtcgag
     1801 gagtaccttt tcctct
//
LOCUS       DAOSIE010000298         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_56101_length_1810_cov_2.304274, whole genome shotgun sequence.
ACCESSION   DAOSIE010000298 DAOSIE010000000
VERSION     DAOSIE010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56101_length_1810_cov_2.304274"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLI69_07130"
     CDS             complement(<1..485)
                     /locus_tag="PLI69_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG42293.1"
                     /translation="MKKQTKQRSPWAWVPTMYFAQGLPYVTVMTLAVVMFKDLGLTND
                     KIALYTSWLYLPWVIKPIWSPIIDLIKTKRMWVILMQTFVSVAFAGIAFSIPTTFFLQ
                     TTMAFFWLMAFSSATYDIAADGFYMLGLSIGDQALFVGIRNTAYRLATIFVEGGLVVL
                     VG"
     gene            complement(499..>1810)
                     /locus_tag="PLI69_07135"
     CDS             complement(499..>1810)
                     /locus_tag="PLI69_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOG42294.1"
                     /translation="TQAELAVGIFSEKEIEFSFNAPYSAGNKSYKGDYRATVKDGKVL
                     FDGELYDTIIFSSNEENANFTLKDVTIGVNFHWERKEDQIFAGKLKLIVEKGRVTAIN
                     LIGIEDYLISVISSEMSATSSKQLLKAHAVISRSWTLAQIVKNKEITASAQEYSTCIE
                     TEDELIKWYDREDHTNFDVCADDHCQRYQGLTRASTEAVREVIEETWGEVLTYEGEIC
                     DARFSKSCGGVFEEFPYCWEDKEMPYLRKQLDNKSGNPIPDLTIEENAREWIYGSPEA
                     FCNTTDMRILSQVLNTYDQETLNFYRWKEKYSQKELSELIKSRSGVDYGEILDLIPVA
                     RGTSGRIWKLKIVGSKRSRTIGKELEIRRTLSPSHLYSSAFVVEKEGTTSSGAPASFT
                     LVGAGWGHGVGLCQIGAAVMGDLGYDYKEILFHYFTGASVDKQY"
BASE COUNT          450 a          436 c          437 g          487 t
ORIGIN      
        1 cctaccagaa cgaccaatcc gccttccaca aaaattgtgg caagcctata tgccgtatta
       61 cgtatgccaa caaatagtgc ctggtcacca atggaaagtc cgagcatata gaatccgtca
      121 gcagctatgt cgtatgtagc gctgctgaat gccatcagcc agaaaaaggc catagtggtt
      181 tgcaggaaga aagttgtggg gattgaaaag gcgatgccgg caaaggccac cgaaacgaat
      241 gtctgcataa ggataaccca cattcgttta gttttgatca ggtctatgat agggctccaa
      301 attggcttga taacccaagg gaggtagagc cagctggtat ataacgcaat cttatcattt
      361 gtcagcccca gatccttgaa cataactacc gcaagagtca tgaccgtgac atagggcaga
      421 ccctgtgcaa agtacatagt ggggacccaa gcccacggag acctttgttt ggtttgtttt
      481 ttcataagtt atatggcttt aatattgttt gtcgacagat gctccggtga aatagtggaa
      541 gagaatctcc ttatagtcat atccaaggtc gcccattacg gctgcgccga tctgacagag
      601 tccgactcca tgtccccaac ccgctccaac gagtgtgaat gaggcgggtg ctcccgatga
      661 tgtagtaccc tctttttcca caacgaaagc ggaactgtag aggtgtgaag gagagagagt
      721 cctgcggatc tccagctcct tgccgatggt gcgggagcgc ttggagccta ctattttaag
      781 tttccaaatg cgacctgaag tgcctctcgc tacggggata aggtcaagta tctcaccata
      841 atccacgccc gaacgactct taatcagctc agagagctct ttctgggagt atttctcctt
      901 ccagcggtaa aagttgagtg tctcctgatc gtaggtattt agcacctggg agaggatacg
      961 catgtcggtg gtattgcaga aggcttccgg agagccgtat atccactcgc gggcattctc
     1021 ctcaatggtt aggtcgggaa tgggatttcc gctcttgttg tcaagttgct tgcgaagata
     1081 gggcatctct ttatcttccc agcagtaggg gaattcttca aaaactcctc cacaggactt
     1141 agagaagcgt gcatcgcaga tttcaccctc ataggtgagc acttcccccc aagtctcctc
     1201 gataacctct cttactgctt ccgtagaggc gcgggtgagt ccctgataac gctggcagtg
     1261 gtcatccgca cagacgtcaa aattggtgtg gtcttcacga tcataccact ttataagctc
     1321 gtcttccgtt tcaatacagg tggaatactc ttgtgccgaa gcggttattt ctttgttctt
     1381 cacaatctgt gcaagtgtcc aggagcgaga gatgactgca tgtgccttga gcagctgctt
     1441 ggaagaggta gcgctcatct cggaggagat cacactgatc aggtaatctt caatacctat
     1501 gagattgatg gcagtcaccc tgcctttctc tacgataagt tttagcttgc ctgcgaagat
     1561 ctgatcttcc tttctctccc agtggaaatt tactccgatg gtgacatcct tgagcgtgaa
     1621 attggcattt tcctcgttgg aggagaagat tatagtatcg taaagctctc cgtcaaaaag
     1681 cacttttcca tctttgacgg tagccctgta gtcaccctta taacttttgt tgcctgcact
     1741 atagggtgca ttgaaactaa attctatctc cttttcggag aaaatgccta cagcaagctc
     1801 cgcttgagtt
//
LOCUS       DAOSIE010000299         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_56379_length_1803_cov_2.729977, whole genome shotgun sequence.
ACCESSION   DAOSIE010000299 DAOSIE010000000
VERSION     DAOSIE010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56379_length_1803_cov_2.729977"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLI69_07140"
     CDS             <1..331
                     /locus_tag="PLI69_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-asparaginase 1"
                     /protein_id="HOG42295.1"
                     /translation="LNTKGLRAVVLESFGSGNAPSMEWFGKLLSESVSRGVLIVNVTQ
                     CHAGSVDMSAYANGAFLMECGLISGYDMTTEAAVTKLFHLLGRFSDNEEVKSYMKRPL
                     KGEFSNK"
     gene            429..1232
                     /locus_tag="PLI69_07145"
     CDS             429..1232
                     /locus_tag="PLI69_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOG42296.1"
                     /translation="MKKFFMFLAVLGLMTVSAQFQVAYAQEETPEVTTEDVQAAPEVD
                     EEEIVDPFAAGASDQPMHQALKQKFIEGGAGFMATILLCLIFGLAIAIERIITLSLAQ
                     TDTKKLIANVDKALKEGGVEKAKDVCRNTRGPVASIFYQGLLRADQGLEAVEKSVTSY
                     GSVQMGQLESGLSWITLFIAIAPMLGFMGTVIGMIQAFDAIEIAGDISPALVAGGMKV
                     ALITTVGGLIVAIILQLFYNYLIAKVDSIVVQMEDASISLVDLLVKHQK"
     gene            1381..1707
                     /locus_tag="PLI69_07150"
     CDS             1381..1707
                     /locus_tag="PLI69_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42297.1"
                     /translation="MDDKILDINLFWAYALIGIGAVLALIFPLAKAFKTKKGVITFLI
                     LIAGAVVVIGGCYLLAPGTPIELNKEVSAATFKFTDTALYVTYVFVAASVIALLWSAV
                     RNSFKR"
BASE COUNT          499 a          357 c          409 g          538 t
ORIGIN      
        1 acttaacaca aaggggctca gggcagtggt cctcgaaagt ttcggcagcg gaaatgcccc
       61 ctctatggag tggttcggaa aattattgtc tgaaagtgtc tccaggggtg tgctcatcgt
      121 caatgtaacc cagtgtcatg ccggaagtgt agatatgtcg gcctatgcca atggagcctt
      181 tctcatggag tgcggactta tcagcggata tgatatgacc accgaggcgg ctgttaccaa
      241 attgttccat cttttgggca ggttttctga taatgaagag gtgaaatcgt atatgaagag
      301 gccattaaaa ggagaatttt caaataaatg aattgttatt aaaaaaaaat aactaaattg
      361 cgcgatattt tactagtcga agaatataac tttaacaaat cgtttaataa tcaattaaaa
      421 cagaacttat gaaaaaattt ttcatgtttt tggcagttct cggcctaatg accgtatctg
      481 cacaattcca ggtagcttat gcacaggaag agactcctga agttactaca gaagatgttc
      541 aggcagctcc tgaagttgat gaagaagaaa tcgttgaccc ctttgctgca ggtgcgtctg
      601 accagcctat gcaccaggcc ctcaaacaga aattcatcga aggtggtgcc ggctttatgg
      661 caacaattct gttgtgtctc atcttcggtc ttgctatcgc tattgagaga atcatcacac
      721 tgagccttgc acagacagac accaagaagc ttattgccaa tgtagataaa gctctcaaag
      781 aaggtggcgt tgagaaggca aaagacgtat gccgcaatac ccgcggtccc gttgccagca
      841 ttttctatca gggtctccta cgtgctgatc agggtcttga agcagttgaa aaatcagtta
      901 cctcatacgg ttccgtacag atgggtcagc ttgaaagcgg tctttcatgg attaccctct
      961 ttatcgctat tgctccgatg ttgggcttca tgggtacagt tatcggtatg atccaggcat
     1021 ttgacgccat tgagatcgca ggtgacatct ctccggcact tgtagcagga ggtatgaagg
     1081 ttgctttgat tacaactgta ggtggtttga tcgttgctat tatcctccaa ttgttctaca
     1141 actatttgat tgcgaaagtt gactccatcg tagttcagat ggaagatgct tctatctctc
     1201 ttgttgacct cctggttaaa catcagaaat agtccatatc cggatcaatc aatagtgtaa
     1261 gacaattatt aacaacgaat taatcatcca attatgaaat atttaaagta tatccaatac
     1321 ttgctgtttg ctgtatcaat agtattgatc ggtgttttct atgcgactca aaccgggtct
     1381 atggatgaca aaatacttga catcaatcta ttctgggcat acgcgcttat aggcatagga
     1441 gcggtacttg cattgatttt cccgcttgca aaagccttta agactaagaa gggtgttatt
     1501 acttttctta ttctgatagc aggcgcggtg gtggtaattg gaggttgcta cctgcttgct
     1561 cctggaactc ccattgagtt aaataaggaa gtctcagcag ccaccttcaa attcactgac
     1621 acggcattgt atgtaacata tgtttttgtt gcggcttcag ttatagcact tctatggagt
     1681 gcagttcgta attcatttaa aaggtaatca agatgggagc acccaaaaag acaccagaag
     1741 ttagtggcgg ttctatggcg gatatctcct tcttgatgtt gatattcttc ttgatggtga
     1801 gta
//
LOCUS       DAOSIE010000300         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_56796_length_1794_cov_3.131110, whole genome shotgun sequence.
ACCESSION   DAOSIE010000300 DAOSIE010000000
VERSION     DAOSIE010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56796_length_1794_cov_3.131110"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /gene="lnt"
                     /locus_tag="PLI69_07155"
     CDS             <1..1220
                     /gene="lnt"
                     /locus_tag="PLI69_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOG42298.1"
                     /translation="HVYFEIELSWPWLVLGNSFATSPSLVQWYEITGSLGGTLWILLT
                     NIIIFSTLTLREKVIVRRLTLAACVLVFIPMAASLIRYYTIDCESGGEKIEVVVVQPN
                     IDPFKKYGILPQEKSDDAMISLVRQVITPQTRYIVTPETFTYDMDLDRPMAHHSFVKY
                     HSFLEEYPNTNLLVGVFSHQIYPTLTPPTHSARRSGDVWYDVFNSAVLMDADNVYSYY
                     HKSKLVPGVEIIPYERYLPFLKKLSSGFGGSGGSYGAQDEMDALEGNDGNKVGPMICY
                     ESVYGNFSRMAVVKGATFMAVMTNDGWWGDTPGYRQHFRYAVLRAIELRRDIVHAANT
                     GISGIINRRGDVIIRTGWWVETSFRGNINTNDSLTPFARYGDIIGLVAAYLFLPFFAG
                     IVLIRIYRRKSKH"
     gene            complement(1209..>1794)
                     /gene="pyrF"
                     /locus_tag="PLI69_07160"
     CDS             complement(1209..>1794)
                     /gene="pyrF"
                     /locus_tag="PLI69_07160"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOG42299.1"
                     /translation="VSYIKEHYPDILVIADAKRGDIGNTAGMYARCFFENMDCDAVTL
                     APYMGEDSVRPFLNYEGKWSVILALTSNNSADDFETLTLAGELSEQLFEKILRTASGW
                     GSKENTMFVVGATRPEKLLGIRAICPDHFLLVPGVGAQGGSVEDVARYGLNDHCGLLV
                     NSSRNIIYASPGEDFAERAAEEAEKLASKMASVL"
BASE COUNT          457 a          421 c          408 g          508 t
ORIGIN      
        1 aacatgttta ttttgagata gagttatcct ggccttggct ggttctcggc aattcttttg
       61 ccacctcgcc ctctctggtg caatggtatg agatcaccgg ctccctcggc ggaacattat
      121 ggatacttct gacaaatatt attattttct caaccctgac tcttcgtgag aaagttattg
      181 tgcgcaggct tactctggct gcctgcgtac tggttttcat ccctatggca gcatccctga
      241 ttaggtatta taccatagat tgtgagagtg gaggggagaa aatagaggtt gtggtggtgc
      301 agccaaatat cgatcctttc aaaaagtacg ggatacttcc tcaggaaaag agtgatgatg
      361 ccatgatctc acttgtacgt caggttataa cccctcaaac cagatatatt gtcactcctg
      421 aaacctttac ttacgatatg gatctcgacc gtcctatggc tcaccactcg tttgtgaagt
      481 accattcatt cctggaggag tatccgaata ctaacttgtt ggttggagtt ttttcacacc
      541 aaatatatcc tacacttacc cctccaacgc acagtgcccg ccggagtggt gacgtttggt
      601 atgatgtctt taattccgca gtcctgatgg atgcggataa tgtctattct tattaccaca
      661 agtcaaaact tgtacccggt gttgaaatta ttccatacga acgttacctg cctttcctta
      721 aaaagctctc gtccggtttc ggtggttccg gaggaagtta tggggctcag gatgagatgg
      781 atgcacttga gggaaatgat ggtaacaagg tcggtcccat gatatgctat gaatcggtat
      841 atggaaattt ttcccgtatg gccgtagtta aaggtgccac cttcatggcg gtgatgacaa
      901 atgatggctg gtggggggat actccgggct accggcagca cttcaggtat gcagttctaa
      961 gggctatcga gttgcgtcgc gacattgtac atgctgccaa taccggcata tccggtatta
     1021 tcaaccgtag gggagatgtg attattcgca ccggatggtg ggtagagacc tctttcagag
     1081 gaaatatcaa tacaaatgac agcctgacac catttgcccg ttacggggat atcataggtc
     1141 tggttgcagc ctatcttttc ctaccttttt ttgcagggat cgtcttaatt cggatttatc
     1201 gtcgcaaatc aaagcactga agccattttc gaagccagtt tttccgcctc ttcagcagca
     1261 cgctccgcaa aatcttctcc cggggaggca taaatgatat tgcgagagga gttgaccagc
     1321 aatccgcaat ggtcatttaa accatatcgg gcaacatctt ccacactgcc tccctgcgct
     1381 cctacaccag gtacaagtaa aaagtggtcc ggacaaatag cacgtatccc aaggagcttt
     1441 tcgggacggg tggcacccac cacaaacata gtgttttctt tggaacccca tccggaggca
     1501 gttcgtagta tcttttcaaa tagttgttct gatagctctc ccgccaaagt gagagtttcg
     1561 aaatcatctg ccgaattatt ggaggtcaga gcaagaatta ccgaccattt gccttcataa
     1621 ttgagaaatg gccgtaccga atcctcaccc atatagggag caagagttac cgcatcgcaa
     1681 tccatatttt caaaaaaaca acgggcatac atcccggcag tattccctat atcacctctt
     1741 tttgcatcag cgattaccaa aatatcggga tagtgttcct tgatataact gact
//
LOCUS       DAOSIE010000301         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_56884_length_1792_cov_3.800230, whole genome shotgun sequence.
ACCESSION   DAOSIE010000301 DAOSIE010000000
VERSION     DAOSIE010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56884_length_1792_cov_3.800230"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..462
                     /gene="rpsH"
                     /locus_tag="PLI69_07165"
     CDS             67..462
                     /gene="rpsH"
                     /locus_tag="PLI69_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOG42300.1"
                     /translation="MTDPIADYLTRVRNAYLAGHKTVEIPSSKMKMEITRILHEKGYI
                     LAYKLVEGKPFNTIKIALKYHPETNKAAIKKIIRISSPGLRQYTNVAKMPRVLNGLGI
                     AILSTSKGIITNKEARDLNVGGEVLCYVY"
     gene            492..1052
                     /gene="rplF"
                     /locus_tag="PLI69_07170"
     CDS             492..1052
                     /gene="rplF"
                     /locus_tag="PLI69_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOG42301.1"
                     /translation="MSRIGKLPVNLPAAVSVSIEEGNVVKVKGPLGELTQKVDPDIKV
                     SVEAGVLTVKRPTDQPRHRSMHGLYRALINNMVIGVSEGFTVEQQLVGVGYHVAVEGQ
                     TVVFNLGFSHEIHFRLPAEIKAEAPAVKKGQNPVLIMKSHDKQLLGMVAAKVRSLRKP
                     EPYKGKGIKFVGEQLRRKAGKSASAK"
     gene            1067..1429
                     /gene="rplR"
                     /locus_tag="PLI69_07175"
     CDS             1067..1429
                     /gene="rplR"
                     /locus_tag="PLI69_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOG42302.1"
                     /translation="MALNKIERRKRIHYRIRKIVNGTPERPRMVVFRSNKQIYVQVID
                     DVNAVTLASAASNDKALVEQCKGKTGVEAAKIVGKAVAERALAKGITTVSFDRGGYLY
                     HGRVKSLADAAREGGLKF"
     gene            1439..>1792
                     /locus_tag="PLI69_07180"
     CDS             1439..>1792
                     /locus_tag="PLI69_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOG42303.1"
                     /translation="MANSNVKRVKTTDLELKDRLVAVRRVTKVTKGGRNFSFAAIVVV
                     GDENGVVGYGLGKANEVTTAISKGIEDAKKNLVRIPLSKRTIPHEQSAKFGGARVFMK
                     PAAPGTGVKAGGAMRA"
BASE COUNT          539 a          385 c          451 g          417 t
ORIGIN      
        1 cattaattat attaacattg gatatcgggc gtccgcatgg gcgtcgaatc ttaaaaacaa
       61 acaacaatga ctgatccaat agcagattat ttgactagag ttcgaaatgc atatctcgct
      121 ggtcacaaga cagtggagat cccctcttcg aagatgaaaa tggagataac ccgtattctc
      181 catgagaagg gttacatcct tgcttataaa cttgtggaag gcaaaccttt caacactatc
      241 aagattgctc tcaagtatca ccctgagacg aataaagcag caatcaagaa gattatccgc
      301 atcagctctc ccggtctgag gcagtacacc aacgtggcaa agatgcctcg cgtactcaat
      361 ggtctgggaa tcgcaattct ctccacttcc aagggtatta taaccaacaa ggaagccaga
      421 gatctcaatg tgggtggtga agtgctttgc tacgtttact aacagttaca aatacaaata
      481 aaatatacaa aatgtcaaga attggaaaat tacctgtaaa cctgcccgca gctgtctctg
      541 tctctataga agagggcaac gtggtgaagg ttaaaggccc gctcggagaa ttgacccaga
      601 aggttgatcc ggatatcaag gtatccgttg aggcgggagt ccttacggtg aaacgtccta
      661 cagaccagcc tcgccaccgt tcgatgcacg gcctctaccg tgcccttatc aacaacatgg
      721 ttataggcgt atctgaaggt ttcacagtag aacaacaatt ggtgggtgtc ggttaccacg
      781 ttgcggtaga gggccagacc gtggtcttca accttggttt ctcccacgag attcacttcc
      841 ggcttccggc tgagatcaag gcggaggctc ctgctgttaa gaagggccag aacccggttt
      901 taattatgaa aagccacgac aaacagcttc ttggtatggt agcggccaaa gtgcgctcac
      961 tgcgtaagcc tgaaccttac aagggcaagg gaatcaagtt cgtaggcgaa caactacgtc
     1021 gtaaagccgg taaatcggcc agcgccaaat aataggagat taagttatgg cattgaataa
     1081 gatagaaaga agaaagagga ttcactaccg catacgcaaa attgtaaacg gtactcccga
     1141 aagacctagg atggttgtct tcagaagtaa caagcagatc tatgttcagg taatagatga
     1201 tgtaaatgcc gttactctcg catcggcagc ctccaatgac aaggctcttg tagagcaatg
     1261 caaaggcaag accggtgttg aggctgcaaa gatcgtcggc aaggcggttg cggagcgtgc
     1321 actagcaaaa ggtatcacca cagtatcttt cgatcgcgga ggttaccttt atcacggaag
     1381 agttaaaagt ttggcagatg ctgcccgcga gggtggtcta aaattctaag gagatattat
     1441 ggcaaattct aatgtaaaga gagtaaagac tactgatctt gagttgaaag atagattggt
     1501 agctgttcgt agagtaacca aggtaacaaa gggtggacgt aatttcagtt tcgccgccat
     1561 tgttgtcgta ggtgacgaga acggtgtggt aggatatggc cttggaaagg caaatgaagt
     1621 gactaccgca atctccaaag gtattgaaga cgcaaagaaa aacctcgtaa ggatccctct
     1681 tagcaagagg acaattcccc atgagcagtc agccaaattt ggtggagcaa gagtatttat
     1741 gaaacctgcg gcccccggta ccggagtaaa ggccggtggt gctatgcgtg cc
//
LOCUS       DAOSIE010000302         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_57100_length_1787_cov_3.345843, whole genome shotgun sequence.
ACCESSION   DAOSIE010000302 DAOSIE010000000
VERSION     DAOSIE010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57100_length_1787_cov_3.345843"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLI69_07185"
     CDS             complement(<1..214)
                     /locus_tag="PLI69_07185"
                     /inference="COORDINATES: protein motif:HMM:NF037184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42304.1"
                     /translation="MISFFKCSTGTIVAVKSDIKLSNDNIQALTWLFSGAEHLGTENI
                     EGFFAGPRREMVTPWSTTATEIAHNMN"
     gene            317..1657
                     /locus_tag="PLI69_07190"
     CDS             317..1657
                     /locus_tag="PLI69_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOG42305.1"
                     /translation="MEFSESLYDEMLEWLFTRFPSYQRVGDRAFKPGLATMREFISQL
                     GEPQSAYSVIHIAGTNGKGSVSHMTASALACCGMKVGLYTSPHLRDFRERMKIISKGT
                     TGCSYEMISKEEVFAFMERYRDFLDTYNPSFFEITTAMAFDWFRSRGVDLAVIECGLG
                     GRLDSTNVVTPLLSVITNIGLEHREYLGHTLEEIAREKAGIIKRGVPALIGERQPETS
                     KVFEEIAAEVASSGLFFAEELPLFLPLSPEEIDLKGDYQQRNLLTVSAIFSLLIGKGL
                     LHVADSGCDIAALSYGVSNAATFTGLHGRWETLRKRADSGGIAGKASIICDTAHNAHA
                     MRWVREQIDKISCDYDNIFFILGVVADKDVDGIASYLPRDVNFILTNSEGERALPAAK
                     LADRLSDHGINGRITHSVSEALEMADSLACEEDLIFICGSNFVVAEVLEIVCSV"
BASE COUNT          449 a          333 c          477 g          528 t
ORIGIN      
        1 tattcatatt atgtgcaatc tccgttgcag tggtactcca gggagtcacc atctctctcc
       61 tggggccggc gaaaaaaccc tcaatatttt ccgtaccgag gtgttcagct ccggagaaga
      121 gccaagtcag agcctgaata ttgtcattgc ttagttttat atcgctctta acggctacga
      181 tagtgcctgt actacatttg aagaatgaaa tcatatcaca agagattttg gaaatatcat
      241 acaaaagtag tgctttttga gagatatatc agttttattc cccaaatttg tgacaaggat
      301 ataaacaagt tatcaaatgg agttttcgga gagtctgtat gacgaaatgc ttgaatggct
      361 tttcacacgt tttccctcat atcagagagt aggtgatagg gcttttaagc ccggtctggc
      421 cacaatgcgt gaattcattt cccaacttgg agagcctcag tcagcctaca gtgtaatcca
      481 tatagccggc accaatggta aaggttcggt ttcacacatg actgcttcgg cccttgcatg
      541 ctgtggaatg aaggtgggac tctatacttc tcctcacctt agagatttcc gcgagaggat
      601 gaaaatcata tcgaaaggga cgacaggttg ttcctatgag atgatctcaa aagaggaagt
      661 atttgctttc atggagagat accgtgactt tcttgatact tacaacccct cattctttga
      721 aatcaccacg gctatggcat ttgactggtt tcgtagcaga ggggttgatt tagctgtgat
      781 agaatgcggg ctggggggcc gtctggactc cacaaatgtg gtaactccat tgctctcggt
      841 gattaccaat ataggattgg agcatcggga atatcttggt cacacattgg aagaaatagc
      901 tcgtgaaaaa gcgggtatca tcaagagagg ggtacctgca cttataggag agcgccagcc
      961 ggagacaagt aaggtctttg aagagatcgc tgctgaggtg gcctcttcgg gactcttttt
     1021 tgccgaggaa cttccacttt tcttgccttt gtcccctgaa gaaatagacc tcaaaggaga
     1081 ttaccaacag cgcaatcttc tgacagtttc tgccatcttt tcattattaa ttggaaaagg
     1141 attgctccat gtcgcggata gcgggtgtga tattgcagcg ttgtcttacg gagtctcaaa
     1201 tgccgccacc tttaccggtt tgcatggaag atgggagact cttagaaaga gggcagattc
     1261 aggcggtatt gcaggtaagg cttctataat atgtgatact gcccacaatg cccacgctat
     1321 gcggtgggtc agggagcaga ttgacaagat ttcttgtgat tatgacaata ttttctttat
     1381 attgggagtg gttgcggaca aggatgtgga tggcatagct tcctatctgc cccgggatgt
     1441 caactttatt ttgaccaata gtgaagggga gagggctttg cctgctgcaa aactggctga
     1501 caggctttcg gatcacggca taaacggtcg tataacccat tcagtcagtg aggcgctgga
     1561 gatggcggat tcacttgcct gtgaagagga tctgatattt atttgcggca gcaattttgt
     1621 tgtggcagaa gtgttggaga tagtttgtag tgtgtagtgt gtagtgtgta gtgtgtagtg
     1681 tgtagtgtgt agtgtgcagt gtgtagtgtg tagtgtgtag tgtgtagtgt gcagtgtgta
     1741 gtgtgcagtg tgtagtatgt agtatgtagt atgtagtgtg tagtgtg
//
LOCUS       DAOSIE010000303         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_57725_length_1774_cov_3.105876, whole genome shotgun sequence.
ACCESSION   DAOSIE010000303 DAOSIE010000000
VERSION     DAOSIE010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57725_length_1774_cov_3.105876"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1654)
                     /locus_tag="PLI69_07195"
     CDS             complement(62..1654)
                     /locus_tag="PLI69_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase family protein"
                     /protein_id="HOG42306.1"
                     /translation="MKFKKLFPILAVVLLTFSCNTNNSNIDDIADDEGVYGYATYTDG
                     RPIAGMVVSDGFSAVITDKDGFYSIESPSPHAFYIFASIPSDCKIVENSEGNPDFYHK
                     YTADRSRYDFKFERMAVENDFVVYALADPQCKDNVHIARFESETIADILKTQKSESLP
                     IYGVTLGDIVYSEGKRNCAPYMAEMRKVMSSRNIGFPVFQTMGNHDVFASATEPIVAQ
                     DFNLGYQRDFESIFGPVNFSWNRGSVHFVHMRNVIFRSNTVYDSYEDGFTMAQVEWLR
                     QDLKYVPKDKLVVLCVHIPLLKNSDGNIHLVRFALSDFDNAIVFSGHTHYMRNEPDLP
                     LPEKVHAAVSGAWWHSVLNGDGSPNGYGIHYFSGNKIVNSIYKGVNGKMASREYQIRM
                     YRGNAKTGGKYEKFQFPLTPGDVVASIFNSSSAWHMKVFEDGVYSGDMQRIPAKRYTG
                     DVIGADSSQDWWAIGYNVGVVGRGHVSGTRSNYMTSSYGTWKYTLKNPEADVRIEATD
                     GYGNKYVCTHFTPDFDYSDLGE"
BASE COUNT          521 a          406 c          364 g          483 t
ORIGIN      
        1 attttgatat cacaaagata tctatatttt taaaactcca attaaaaacc acacacattc
       61 attactcccc taaatctgaa taatcgaagt cgggtgtgaa atgtgtacag acatatttgt
      121 tgccatatcc atcagtggct tcgatacgga catcggcttc aggattttta agagtatatt
      181 tccaggtacc gtaacttgaa gtcatatagt tggagcgggt gccggaaaca tgaccacgac
      241 cgactacgcc tacattgtat ccgattgccc accaatcctg acttgagtcg gcacctatga
      301 catcacctgt atagcgtttg gcaggtatcc tttgcatatc cccgctgtaa acgccatctt
      361 caaaaacctt catatgccag gcggaggaac tgttgaaaat agaggctaca acatctccgg
      421 gtgtaagtgg aaactgaaac ttttcatatt tcccgcctgt tttggcatta ccgcgataca
      481 tgcgtatctg atactccctg gaggccattt taccatttac acctttgtaa atggaattga
      541 caattttgtt tccgctaaaa taatggatcc cgtagccgtt gggcgaaccg tccccattga
      601 gaacggagtg ccaccaggct ccgctgacgg ctgcatgaac cttttcagga agtggcaaat
      661 caggctcatt acgcatataa tgagtatgcc cggagaaaac aatggcattg tcgaaatcag
      721 agagtgcaaa cctcaccagg tggatattgc catcactgtt tttgagtagc ggaatatgaa
      781 cacaaagcac caccagtttg tcttttggaa catacttcaa atcctgtctt agccactcca
      841 cctgagccat cgtaaaacca tcttcatacg aatcatagac ggtattgctc cggaatatga
      901 catttctcat atggacgaaa tgcactgaac ctctattcca ggaaaaattg acgggaccga
      961 agattgactc gaaatctcgt tgatagccca aattgaaatc ctgagccact atgggttcgg
     1021 ttgcgcttgc gaagacatcg tggttgccca tagtctgaaa caccggaaaa ccaatatttc
     1081 gcgatgacat cactttgcgc atttcggcca tatagggagc acaattgcgt tttccctcag
     1141 aatagacaat atctcccaga gtgaccccgt aaataggcag gctctcactc ttctgtgtct
     1201 tcaaaatatc cgcaattgtc tccgactcga agcgcgcaat gtgaacattg tccttgcatt
     1261 gcggatcggc aagcgcataa accacaaagt cgttctccac tgccatgcgc tcgaatttga
     1321 aatcatatct gctccgatct gccgtgtatt tgtgataaaa atcgggattg ccttcggaat
     1381 tttcaactat cttacaatct gacggaattg atgcaaatat gtaaaaagcg tggggtgatg
     1441 gtgactcaat gctataaaag ccatctttat cggtgattac tgcactgaag ccatcgctaa
     1501 cgaccattcc tgcgataggg cggccatctg tgtatgtggc gtagccgtaa actccctcat
     1561 cgtcggcaat atcgtcaata ttggagttgt tggtgttaca ggaaaaggtc agcagtacga
     1621 ctgcaagtat cggaaatagc tttttaaact tcatttgggt tgttaatgaa aattgtgata
     1681 ccattttgat atcacaaaga tatcaaaaat tgcaaaattc caaaaaaaat aaaaaaaata
     1741 aagatgccca aacaaaagtc gggcatcttt agta
//
LOCUS       DAOSIE010000304         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_58000_length_1767_cov_3.790304, whole genome shotgun sequence.
ACCESSION   DAOSIE010000304 DAOSIE010000000
VERSION     DAOSIE010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58000_length_1767_cov_3.790304"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /gene="cls"
                     /locus_tag="PLI69_07200"
     CDS             complement(<1..1356)
                     /gene="cls"
                     /locus_tag="PLI69_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOG42307.1"
                     /translation="MTWTLFWKICSFALYFANISLVIYVVWRLVMKKSDPVKTLTWIV
                     VLILLPYIGLILYLLMGQNYRKDAIFKNKRIVDVEVRKKLALNQLQHYKELDNLPASV
                     FPYKKLIRQNLLGNYSILTVIDKIDFYFTGKEALDAMIADMESASQHIHLQSFIFMDD
                     NIGTRIRDLLVKKAAEGVEVRVIYDSVGSIKTKRRFFKEMHQEGIEVLEFSPVRIFRP
                     TYKINYRNHRKILVIDGRVGYIGGINIADRYYDGGIYEEWRDTQIRVEGEAVFPMQSS
                     FLLDRYFTINRHLTRRRKYYADIDIENIYRHKAATDIYSQFLTSGPDSDWSSIKGSYF
                     SMITGASRRICINTPYFTPSESLIKAIRTASLGGVEVNLMMPARSDSTLIHYCSMSYI
                     KELLRAGVNVYLFKCGFIHSKSITVDGQICMVGSANMDNRSMLYDFEVMSVIYNPQCA
                     AI"
     gene            complement(1366..>1767)
                     /locus_tag="PLI69_07205"
     CDS             complement(1366..>1767)
                     /locus_tag="PLI69_07205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG42308.1"
                     /translation="FEIASADSLPFADDTFSAVTISFGIRNFDRRSECIDEIFRVTAP
                     SGELMILEFAVPRCRIWRALYIFYLKHILPLIGGWLSGNRSAYRYLPESVMQFPRYEE
                     FCTELTAAGYTDVSYESLMCGVALLYKAHKP"
BASE COUNT          516 a          408 c          383 g          460 t
ORIGIN      
        1 gatagctgcg cactgaggat tgtagattac ggacatcacc tcaaagtcgt atagcatgct
       61 gcggttatcc atgtttgccg aacctaccat acatatttgc ccatccacag ttatgctctt
      121 ggagtggata aagccacatt tgaagagata gacattgact cccgccctta gcaactcttt
      181 gatgtaagac atggaacaat agtgaatcag tgtggaatct gatcttgcag gcatcatcaa
      241 attgacctcc acgccaccca gagatgcggt acggatggct ttaataagcg attccgaagg
      301 ggtaaagtag ggagtattga tgcagatgcg gcggctggcg cctgtaatca tggaaaagta
      361 gctccctttg atgcttgacc agtcgctgtc cggcccggaa gtaagaaact gtgagtaaat
      421 gtcagttgcc gctttgtggc gataaatatt ttcaatgtct atatcggcat aatacttcct
      481 gcgacgcgtc agatgacggt tgatagtaaa atatctgtcg agcagaaagc tggactgcat
      541 tgggaagacc gcctcccctt ccaccctgat ctgggtatcc ctccactctt catagatacc
      601 gccgtcgtaa tagcgatccg ctatgttaat gcctccgata tatcctacgc ggccgtcaat
      661 aaccaatatc ttacggtgat tgcgataatt aattttataa gtgggtctaa agatacgcac
      721 cggcgaaaat tcgagtactt ctattccctc ttgatgcatc tctttgaaaa agcgtctttt
      781 ggtctttata gagccgacac tatcgtagat aacgcgtacc tctactcctt cagcggcctt
      841 tttgaccaac agatcacgaa tacgagtccc tatattatca tccataaata tgaatgactg
      901 taaatgtata tgttggctag ccgactccat atcggcaatc atcgcatcta gcgcctcctt
      961 gccggtgaaa tagaaatcaa tcttgtcgat gactgtaagt atgctataat tgcccagcag
     1021 attctgacgg ataagtttct tatatggaaa gaccgaagcg ggcagattat ccaattcttt
     1081 gtagtgctgc agttggttca aagctagttt tttgcgtacc tccacatcca ctatcctctt
     1141 atttttgaaa attgcatcct tacgataatt ttgacccatt agcaaataga gaattaagcc
     1201 aatatagggt agcagaatca gcaccacaat ccatgtaagc gtctttacgg gatcggattt
     1261 tttcatcaca agtctccata cgacatatat caccagagag atattggcga aatagagagc
     1321 gaaggagcat attttccaga aaagagtcca ggtcataatc tcaatctaag gtttatgagc
     1381 tttatacaaa agagccaccc cacacataag ggactcgtat gaaacatcgg tataacccgc
     1441 agcagtgagt tcagtgcaaa actcctcata acgggggaac tgcatcaccg actcgggcag
     1501 atagcggtaa gcactcctgt tgccggagag ccatcctcca atcaaaggca aaatgtgctt
     1561 taaataaaat atataaagag ctctccagat gcgacaacgc ggcactgcaa actcaagtat
     1621 catcaattct cccgaggggg cggtaacacg gaatatctca tcaatgcact ctgacctgcg
     1681 gtcgaaattg cggatgccga aagagatggt cacagcagaa aatgtatcgt cagcaaaagg
     1741 gagcgaatct gccgaagcaa tctcaaa
//
LOCUS       DAOSIE010000305         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_58397_length_1758_cov_3.411627, whole genome shotgun sequence.
ACCESSION   DAOSIE010000305 DAOSIE010000000
VERSION     DAOSIE010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58397_length_1758_cov_3.411627"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLI69_07210"
     CDS             complement(<1..323)
                     /locus_tag="PLI69_07210"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG42309.1"
                     /translation="MNPTDFLQEAIKGGLKSLYVVELPQTEIQVQATRKEFEGDYTLV
                     VFPLLKISKKSPEATAQEIGAYLEKNCPDVDSFNVIKGFLNIKLAPAFWVRVLQSIGE
                     NEDFGL"
     gene            complement(336..>1758)
                     /locus_tag="PLI69_07215"
     CDS             complement(336..>1758)
                     /locus_tag="PLI69_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HOG42310.1"
                     /translation="HFVEGMSNFWNALGNGITVVSDFICGYPLFFLLIGGGLFFLFYS
                     GFTPLRRYGRAIQALRVKDDKAEGQISSFEALTSAIAATVGMGNIAGVAIAISVGGPG
                     AIFWMWISAIAGMATKFFEGTIAVMYKGKDSEGETQGGTMYMIMTGLGRKWKPLAVFF
                     SIFGLIGTLCIMQSNQLTESVTSLFFAPEQNTLLLRFIIGVIITAIVAGVIIGGIKRI
                     SKVSTKLVPLMVGLYFILVLFIMIKHIGHVPGVFKDIIAGAFTPRGALGGGIGSIIMI
                     ALTGVRRAALVNEAGVGTASMMHGASKNNEPVREGLVAMIGPSIDSGLVCTLTAIAVL
                     IGVRIDPSIMPAGSGMSSMEGLKFAINAFNASIPVVGGYLLFIMVLMFAFSTMFSYSY
                     YGQKCTGFLFGAKNAKYYNIFFLLMLIVSAMIPLKAAVSLIDTAYALMAFPTMFTLFM
                     LAPKAKKQMNIYFAANKKEKKVK"
BASE COUNT          516 a          420 c          355 g          467 t
ORIGIN      
        1 aggccgaaat cttcattttc accgatagat tggagcactc tcacccagaa agcgggagca
       61 agtttaatgt tgagaaaacc ctttatgaca ttgaaagagt ccacatcggg gcagtttttc
      121 tcaagatatg ctcctatctc ctgtgcagta gcctcaggcg actttttact gatcttgagg
      181 agaggaaata cgactagggt ataatcccct tcaaactctt ttctagttgc ctgtacctgt
      241 atctcagttt gggggagctc aacaacatat agactcttga gtcccccctt tatcgcttct
      301 tgtaaaaaat ctgtcggatt catttctttt tttggttact tgactttctt ttccttttta
      361 tttgcagcaa aatagatatt catctgcttt tttgcctttg gtgccagcat aaatagagta
      421 aacatagttg ggaatgccat caaagcgtaa gccgtatcaa tcaggctgac agccgccttg
      481 agcggaatca ttgccgaaac aatcagcatc aggaggaaga aaatattgta atattttgca
      541 ttttttgcac cgaaaagaaa tccggtacat ttttgtccat aataagaata agagaacatc
      601 gtgctgaatg caaacatcaa gaccataata aagagcagat aaccgcctac aacgggaatc
      661 gaggcattga atgcatttat ggcaaacttg agaccctcca tggaactcat tcccgatccg
      721 gcaggcatta tggagggatc aatccttaca cctataagga cggctattgc tgtgagtgtg
      781 cataccaaac cggaatcaat cgaggggcct atcattgcaa ctaaaccttc acgcacgggt
      841 tcgttgtttt ttgaggcacc atgcatcatg gaggcagtac ctactccggc ctcattgaca
      901 agtgcagcac gacgcacacc tgttagggct atcattatta tagatcctat accccctcca
      961 agggctcccc tgggagtgaa tgctcccgcg ataatatctt tgaaaactcc cggcacatgg
     1021 cctatatgtt ttatcataat aaagagtaca agaataaaat agagccctac catcagggga
     1081 accagtttgg ttgaaacctt tgaaattctc ttaattccac ctatgatgac tcccgcaaca
     1141 attgccgtta taattacgcc gataatgaat ctaagcaaaa gtgtgttctg ttcaggagca
     1201 aaaaacagcg aggtaacaga ctctgtcagc tggttggatt gcattataca aagcgtacct
     1261 atcagaccga atatcgagaa aaagactgca agtggtttcc atttacgacc caagcccgtc
     1321 ataatcatat acatggtgcc accctgagtt tcaccttccg aatccttacc tttgtacatc
     1381 acggcaatgg taccctcaaa aaatttagtt gccatgccgg cgatagcact gatccacatc
     1441 cagaatatgg ctccgggacc tcctacggag atggctatgg ctacaccggc aatattgccc
     1501 atgccgactg tcgccgcaat ggcacttgtc aatgcctcga aagaactgat ctgcccttcg
     1561 gccttgtcat ccttgactct aagagcctga atggcccttc catacctacg tagcggtgta
     1621 aatcctgaat agaatagaaa gaataatccg cctcctatga ggagaaaaaa gagaggatat
     1681 ccacaaataa aatcgctaac gacagttatg ccgttaccaa gcgcattcca gaaattgctc
     1741 ataccttcga caaaatgc
//
LOCUS       DAOSIE010000306         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_59342_length_1739_cov_2.737530, whole genome shotgun sequence.
ACCESSION   DAOSIE010000306 DAOSIE010000000
VERSION     DAOSIE010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59342_length_1739_cov_2.737530"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1489)
                     /gene="rpoN"
                     /locus_tag="PLI69_07220"
     CDS             complement(83..1489)
                     /gene="rpoN"
                     /locus_tag="PLI69_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOG42311.1"
                     /translation="MLKQTLQQTQTTKLSPLQIQTIKLLELPMLELEQKIQREIEENP
                     VLDEVIPESEEEESANVALDSYDRDDHTPSYKLHVNNQGKDLKPQYNTFSVQESFHQN
                     LIKQLGFCSLDDRKRQIATFMIGMLEDDGYLRRSLDSLTDDIAFRLGIETTENELEEI
                     LFIIQEFEPVGVGARDLRECLLLQIAAKSPSPSQQLAETILRDYFNEFSRKHYPKIMS
                     RLGITSDELKEAIEEIQRLNPRPGGQIDDSYSEQTQQVIPDFILELREGELVMSMPRY
                     SLPELRVNKRYADMLIKGSNENTKAGKDAVIFVKKKLDDAKWFIEAIKQRQNTLQNTM
                     NAIIDFQREFFMDGDETKLKPMVLRNIAEKANLDISTISRVVNCKYIQTHFGIYPLKY
                     FFSEGLLNEDGEEVSTREIKNVLTRIIDNEDKSSPLTDESIVEALSEKGYKVARRTVA
                     KYRDQLGIPVAKQRVQLL"
BASE COUNT          434 a          367 c          379 g          559 t
ORIGIN      
        1 ctaccggagt ccagatgctc cgactctgtg cgcaagttac ctatataggt tgtcttaatt
       61 aaagtcatag tctgcttaat aattataata attgtactct ttgtttagct accggtatac
      121 ccagttggtc ccggtatttg gcaaccgtac gtctggctac cttataaccc ttttctgaaa
      181 gggcttctac gatgctctcg tcagttagtg gagagctttt gtcctcattg tctataattc
      241 gggtaaggac attctttatc tcgcgggtgg aaacctcctc accatcttcg ttgaggaggc
      301 cttcggagaa gaaatatttt agcggataga tgccgaaatg agtctgaata tatttgcagt
      361 tgactactct ggaaatggta gaaatgtcga gattggcttt ttctgcaatg tttctgagta
      421 ccatcggctt gagtttggtc tcgtcaccat ccataaagaa ttctctctgg aagtcaatta
      481 tggcgttcat tgtattctgc agagtattct ggcgttgctt gatggcttcg ataaaccatt
      541 tggcatcatc aagtttcttt ttgacaaaaa ttacagcatc cttacccgcc tttgtattct
      601 catttgagcc cttaatcagc atatctgcat agcgtttatt gactctgagt tccggcaagg
      661 agtagcgggg catagacatc accaattcgc cctctctgag ttccaggatg aaatcaggga
      721 tcacctgctg ggtttgctcc gaataggagt catcaatttg cccgccgggg cgtggattaa
      781 ggcgctggat ctcttcgata gcctccttga gttcatcgct ggtgataccc agccgggaca
      841 tgattttagg gtaatgcttt ctggagaatt cattgaaata gtcccttaat atggtttcag
      901 ccaattgttg agatggggag gggcttttag cagctatctg tagcaaaagg cactctctga
      961 ggtctcttgc acctactcct accggttcga actcctgtat tatgaagagg atctcttcga
     1021 gttcattttc ggtggtttct ataccgagac gaaaagcgat atcatccgta agagagtcca
     1081 gactgcgcct aaggtaacca tcatcctcca gcatacctat cataaaggtg gcaatctgtc
     1141 tttttctatc gtcgagagag cagaatccga gttgttttat gagattctga tggaagcttt
     1201 cctgaacgga aaatgtgttg tactgtggtt taaggtcttt accctgatta ttgacgtgga
     1261 gtttatatga aggagtgtga tcatcacggt catacgagtc aagggcaaca ttagctgatt
     1321 cctcctcctc ggattccggt ataacctcat ccaggacggg attttcctca atctccctct
     1381 gaatcttctg ttccaattcc aacattggca gctccagaag ctttatagtc tgaatctgga
     1441 gcggagagag cttcgtcgtc tgtgtctgtt gtaatgtctg ctttaacatt tcataacaaa
     1501 ggtagcaatt ttatgtgact gtgcctatct cgccccgaaa aatccttgca atttattcat
     1561 cgttctgaca atttcgttat ctttgggcca taatataatg gagtaatgag cacttataaa
     1621 aaatgtgtct ctatttggaa taataataaa caaaaacaat gcaatttttt aaaaaactga
     1681 tttttatcct gacacttttg tcgggaatgt caatagcagc atattcacag tcggatctg
//
LOCUS       DAOSIE010000307         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_59477_length_1736_cov_3.170732, whole genome shotgun sequence.
ACCESSION   DAOSIE010000307 DAOSIE010000000
VERSION     DAOSIE010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59477_length_1736_cov_3.170732"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="PLI69_07225"
     CDS             <1..1354
                     /locus_tag="PLI69_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG42312.1"
                     /translation="SYTNNYMAQGFFARAQYDYDGRYFVSSSFRRDASSRFAKENRWG
                     NFGSIGAAWLMSEESWLRGVNMVDMLKLKVSYGIQGNDNLGGYYPYADQYTHSYNEDT
                     GEYSLTLSSRGNKDLTWETSKALNVGVDFELFHGYFNGTVEWYARKTEDLLYSKNLPL
                     SMGAPSTMPINVGSIMNTGVEVSLYGNIINTAKVHWTWNINMSHYKNTILELDPSVSE
                     KGIRGSNYIYKVGGSLYDAYMYKYAGVDPENGQALYWKHDDETGEDSTTPVFSDATRY
                     ELGTVMPKLYGGFGTSLTAYGFDFSAQASFQLGGKFYDGGYQSLMWTQKSAGSAWHKD
                     ALKAWTPENPNTDVPRMDGDTQVAQSAVDRFLITSNYLSLNNVTLGYTFPKSLLNKIK
                     VAGLRVYVAGENLGVLAARKGIDPRFSMGLGSYTSGSGIGTSYYSAMRTITGGITLTF
                     "
     gene            1380..>1736
                     /locus_tag="PLI69_07230"
     CDS             1380..>1736
                     /locus_tag="PLI69_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42313.1"
                     /translation="MKINKLLLLISLAVGLAVTACADMDNVLPESGTLLESQLQETNA
                     IAPQRAQAAFSGLFSDIGKPAKMYSTPDDWGFLMINFCNDLEGPDALIADNNYNWFGV
                     CGELSSRNANYRNPAIR"
BASE COUNT          460 a          410 c          400 g          466 t
ORIGIN      
        1 atcatacacc aacaactaca tggctcaggg tttctttgca cgtgcacagt atgattatga
       61 cggcagatac tttgtaagtt cctctttccg tcgtgatgca tcttcacgtt ttgcaaaaga
      121 gaaccgctgg ggtaacttcg gtagcatagg cgcagcatgg cttatgagcg aagagagctg
      181 gcttagagga gtcaacatgg tggatatgct taaactcaag gtgagctacg gtatccaggg
      241 taacgacaac cttggtggat actatcccta tgcagatcag tacacccact catacaacga
      301 ggacacaggt gagtactcgc tgactctctc ttcaagaggt aacaaagatc ttacctggga
      361 gacctcaaaa gccttaaacg taggtgtaga ctttgaactt ttccatggtt atttcaacgg
      421 tacggttgaa tggtatgccc gtaagaccga agaccttctc tattccaaga accttcctct
      481 ctcaatggga gctccatcca caatgcctat caacgtaggt agtattatga ataccggtgt
      541 tgaggtttct ctctacggta atatcatcaa tacagcgaag gtacactgga cctggaatat
      601 aaatatgagc cactacaaga acacaatcct tgaactggat ccaagcgttt cagagaaggg
      661 tatcagggga tccaactata tctacaaggt aggcggttct ctatatgacg cgtatatgta
      721 caaatatgca ggcgttgatc ccgagaatgg tcaggccctc tactggaaac acgatgatga
      781 aacaggcgag gattctacca ctccggtatt ttccgatgct acaaggtatg agctaggtac
      841 agttatgccc aaactctatg gtggtttcgg tacctctctt actgcatacg gctttgactt
      901 ctcggcacag gcttcattcc agttgggagg taagttctat gacggtggtt atcagtcttt
      961 gatgtggaca caaaagtctg caggaagtgc ctggcacaaa gatgctctta aggcatggac
     1021 ccctgagaat cccaatacgg acgttccccg tatggatggt gacactcagg ttgctcagag
     1081 tgcggttgac cgcttcctca ttacttcaaa ctacctcagc ctcaataacg tgactttggg
     1141 ttatacattc cctaagagcc ttcttaacaa gattaaggtg gcaggacttc gcgtttacgt
     1201 agcaggagaa aaccttggag tgcttgctgc tcgcaagggt attgaccccc gtttcagcat
     1261 gggtcttggc tcctacacat caggctccgg tattggtacc agctattatt cagctatgcg
     1321 caccatcacc ggcggtatca ctcttacatt ctaatcacta catctaacaa ggtattaaaa
     1381 tgaaaataaa taaattatta ttactcatat cattggcagt cggactcgca gttacagctt
     1441 gtgccgacat ggacaatgtt cttccagaga gtggtacgtt gttggagtca cagctccagg
     1501 agacaaacgc aattgctcct cagcgtgctc aggcagcttt ctccggtctc ttttcagata
     1561 tcggtaagcc ggctaagatg tatagcactc cggatgactg gggttttctg atgatcaact
     1621 tctgtaatga tctcgaaggc cctgatgcac tgattgccga taacaactac aactggttcg
     1681 gtgtctgcgg tgaactctct tcacgtaacg caaactaccg taatcctgcc atccgt
//
LOCUS       DAOSIE010000308         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_59986_length_1726_cov_2.710353, whole genome shotgun sequence.
ACCESSION   DAOSIE010000308 DAOSIE010000000
VERSION     DAOSIE010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59986_length_1726_cov_2.710353"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1726)
                     /locus_tag="PLI69_07235"
     CDS             complement(<1..>1726)
                     /locus_tag="PLI69_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOG42314.1"
                     /translation="IETNTFNSNAISLAEYNLQDKAYKLNLKGARIACRAAAGATARQ
                     NSRRVFVAGCIGPTSVMLSLSPDVSRPDYRAVSFEEMALAYSEQVRGLIDGGADLLLV
                     ETIFDSINARAALYAISSVQQEMGTSLPVMVSATVNDCNGNILTGQSVEALYHTLAGH
                     NLLSFGLNCSFGAKDLKPIITALSEILPCAISIYPNAGLPNEMGAYDETPRMTATLLK
                     EMALEGMLNIAGGCCGTTPDHIRAIDEALKGIPPRIIEPRMIEERYTSAPLIVTGLRT
                     VVVDRREQNFINVGERTNVAGSRKFARLIKERQYEEAASIAEKQIEDGASIIDINMDD
                     PMLDSAVEMGKFLRYIANDPKIAKAALMIDSSDWETLLEGLRWSPGKSIVNSISLKEG
                     EEEFLRKAAEIKKFGAAVIVMAFDEEGQAVTFQRKIEICRRAYELLTQRGGYAPNDII
                     FDANILTIATGIKEHNRYAVDFIEAVRWIKSNLPGCHTSGGLSNISFSFRGNNTIREA
                     MHSVFLYHAIDAGLDMAIVNPSMLRIYSTIEPSLLKAVEDVVLDRDPEATARLVDLAE
                     SLKADTQDS"
BASE COUNT          416 a          463 c          398 g          449 t
ORIGIN      
        1 tgaatcttgt gtgtccgcct tcagactctc ggccaaatcc accaatcttg ccgtggcttc
       61 gggatcccgg tcaagcacca catcctctac cgcctttagc agagatggct ctatagtgct
      121 gtatatccgg agcattgaag gattgacaat tgccatatcc agccccgcat caatagcgtg
      181 gtagagaaat accgagtgca tcgcctctcg gatggtattg ttgcctctga aggaaaaaga
      241 gatattggag agtcctccgg aggtgtgaca gccgggaaga ttggacttga tccagcgcac
      301 agcttcgata aaatccacgg cataacgatt gtgctccttg ataccggtag caatggtaag
      361 tatatttgcg tcgaaaatta tatcgtttgg cgcatacccg cccctctgcg taagcagctc
      421 ataagccctt cggcaaatct caatcttgcg ctgaaacgtg actgcctgac cctcctcatc
      481 aaaagccatc acaatcacgg cagctccgaa cttttttatc tcagccgcct ttcggaggaa
      541 ctcctcctcc ccctctttta gcgaaatgga gttgactatg ctcttgccag gagaccagcg
      601 caacccctcc aaaagagtct cccagtcaga ggagtcaatc attaaggcag cttttgcgat
      661 tttagggtca ttggctatat atctgaggaa ctttcccatc tctacagcac tatccaacat
      721 aggatcgtcc atattgatat ctatgatcga ggcaccatcc tctatctgtt tttcagctat
      781 ggaagctgcc tcttcatatt gccgttcctt tatcagacgg gcaaatttcc tgctgccggc
      841 aacgttggtg cgctctccga cattgataaa attctgctcc cgcctatcca cgaccactgt
      901 tctgagcccc gtaactatca aaggagccga agtgtacctc tcttcaatca tgcgcggctc
      961 tattattcgc ggagggatac cctttagtgc ttcgtctata gcgcggatgt gatcgggcgt
     1021 ggtgccgcag catccccctg ctatgtttag cattccctcg agtgccatct cctttagaag
     1081 agttgcggtc atccggggag tctcgtcata cgcccccatt tcgtttggta ggccggcatt
     1141 tggatatatg gagattgcac aaggcagtat ctctgaaagt gcagtaataa ttggcttgag
     1201 atccttggca ccaaaggagc aatttagtcc gaaactgagc aaattatggc cggcaagggt
     1261 atggtagagg gcctccacgc tttgtcctgt aagaatatta ccgttgcagt cattaaccgt
     1321 cgcagatacc attaccggta aactcgttcc catctcttgc tgaaccgagg agattgcata
     1381 aagtgcagct ctcgcattga tggagtcaaa tatcgtctcc accagcagca ggtctgcccc
     1441 tccatcgatg agacccctca cctgctcaga ataggctaag gccatctctt caaatgaaac
     1501 tgcgcgataa tcgggcctgg atacatccgg agagagagag agcattacag aagtcggacc
     1561 tatgcatccg gctacaaaga ctcttcgcga attctgcctc gccgtagcac ctgcagcagc
     1621 cctgcatgca atacgtgctc ccttgagatt gagtttataa gccttatcct gcagattgta
     1681 ctccgcgaga gaaatagcat ttgaattgaa ggtgttggtc tcaatg
//
LOCUS       DAOSIE010000309         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_60013_length_1725_cov_4.395210, whole genome shotgun sequence.
ACCESSION   DAOSIE010000309 DAOSIE010000000
VERSION     DAOSIE010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60013_length_1725_cov_4.395210"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="PLI69_07240"
     CDS             complement(<1..1567)
                     /locus_tag="PLI69_07240"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOG42315.1"
                     /translation="MTPIFYLIPVCSILALVFAYVFYRRMKKADEGTPTMREIASHVR
                     KGAMAYLKQQYKVVTIVFLILALFFAILAYGFGVQNPWVPFAFLTGGFFSGLAGFIGM
                     KTATFASSRTANACRTSLNSGLRVAFRSGAVMGLTVVGLGLLDISLWYLILNAFIETT
                     PEQKLVVITTTMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKLEENIPEDDPRN
                     PATIADNVGDNVGDVAGMGADLYESYCGAILATMALGASAFFSKGYSAQLNAVFAPML
                     IAAVGIVLSILGIFTVRTKENAGMDKLLKSLSLGVNLSAVFIAIFTFVILWLLDLENW
                     FWISCSVIVGLLAGIIIGRSAEYFTSHSYKPTRRVAESGKTGPATVIISGFGVGMIST
                     AIPVITVAVAIILAFLFAIKFDISAMLSAHNLSIGLYGISISAVGMLSTLGITLATDA
                     YGPIADNAGGNAQMSNLDPEVRKRTDVLDALGNTTAATGKGFAIGSAALTALALLASY
                     LEEIKIGLANIGQT"
BASE COUNT          498 a          404 c          396 g          427 t
ORIGIN      
        1 ctgtctgccc gatgttcgca agaccgatct taatctcctc caaataggat gctaacaaag
       61 caagcgctgt caaagctgcc gagccgatgg caaatccttt acctgtagca gcagtggtgt
      121 tgccaagagc atcaaggaca tcagtcctct tccgcacttc cgggtcgaga ttgctcatct
      181 gcgcatttcc tccggcattg tccgctatgg gaccgtaggc gtcagtagca agggtaatgc
      241 ccagagtcga aagcataccc acagcagaaa tgctgatacc gtaaagccca atacttaggt
      301 tgtgcgctga aagcattgca gatatatcaa atttgatagc aaataggaat gcaagaatga
      361 tcgccaccgc aacagttatg accggtattg cggttgatat cataccgact ccaaatcccg
      421 agataatcac agtagctgga cctgtctttc cgctttctgc aaccctgcgg gtaggcttgt
      481 aagagtgtga agtgaagtat tctgccgaac gtcctattat gatacccgcc agcaatccta
      541 caattaccga acaagagatc caaaaccagt tttccagatc gaggagccac aatatgacga
      601 aagtgaagat cgctatgaat accgcactaa gattaactcc aagactgagc gatttgagga
      661 gcttatccat tccggcattt tctttggtac gtactgtaaa gattcccagg attgatagca
      721 cgattccgac cgctgcaatt aacatcggtg caaagacggc attcagctgc gcactatatc
      781 cctttgaaaa gaaggcggaa gcacccagag ccatagtggc gagaattgcg ccacaataag
      841 attcataaag atccgctccc atacctgcca cgtctcccac attatcacca acgttgtcag
      901 caatagttgc agggttgcgc ggatcatctt cggggatatt ctcctccaat tttccaacca
      961 ggtcagctcc cacatcggcc gctttggtgt aaataccgcc accaactctc gcaaatagtg
     1021 cttgggtgga agcacccatg ccgaaagtaa gcatcgtggt agtaatgaca accaattttt
     1081 gctccggagt agtttcaata aaagcattca gaataagata ccaaagtgat atatccaaca
     1141 gaccaagtcc cactacggtc aagcccatca cggctcccga acgaaatgcc accctaagtc
     1201 ctgaattaag agaagttcta caagcatttg cggtacgtga agaggcaaag gtcgctgttt
     1261 tcatccctat gaatccggca aggcctgaga aaaagcctcc cgtcaaaaat gcaaagggta
     1321 cccaagggtt ttgtactcca aatccatatg caagaatggc aaaaaataga gcgagaatga
     1381 ggaatacgat ggttactacc ttgtactgtt gctttaaata ggccatagcc cccttcctga
     1441 catgagaggc tatctccctc atagttggag tcccttcatc cgccttcttc atacggcgat
     1501 aaaaaacata agcaaatacc aatgccaaaa tcgagcatac cggaattaaa taaaagattg
     1561 gagtcatgat ggtgtagttt tgagttctga gtgtgtagtt atgagttagt ttagagtttg
     1621 gagtatgtag ttaggagttg gtgattagtt tggagttggg agtgtgtagt gtggcttggt
     1681 taggttagtg tgaattcgtg caacgtactg taatgtgtag tgtac
//
LOCUS       DAOSIE010000310         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_60670_length_1713_cov_3.762967, whole genome shotgun sequence.
ACCESSION   DAOSIE010000310 DAOSIE010000000
VERSION     DAOSIE010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60670_length_1713_cov_3.762967"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PLI69_07245"
     CDS             <1..1042
                     /locus_tag="PLI69_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOG42316.1"
                     /translation="GDKWCIYPMYDYAHGQCDSIERITHSICTLEFDVHRPLYDWFIE
                     NLEIFPSRQYEFARLNLTYTVMSKRRLLELVRNNIVEGWDDPRMPTICGLRRRGYTPE
                     SIRLFADKVGVAKRDNVIDLSLLEWCLRDDLNRRSNRYMAVINPIKLVIDNWDEGKVE
                     YFEAPLNPEYPDGPKRRVAFTKELYIESEDFMEEAPKKYHRLKPDGEVRLKYSYIVKC
                     VDYIKNEAGEVVEIHCTYDPASKPGAGPWRSVKGTIHWASVTHSEKITVNLFDKLFTE
                     EQMGSIPEGKDYRDYINPDSLIVKEAFAEPDLLKDDSGMAVQFERMGYFIKDPVNGGY
                     NKATGLKDSFKL"
     gene            complement(1032..>1713)
                     /locus_tag="PLI69_07250"
     CDS             complement(1032..>1713)
                     /locus_tag="PLI69_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOG42317.1"
                     /translation="SEDPAIFSWQICNEPRPFSSGENDNRSLFAEWIKETAQLIRSID
                     PNHMISTGSEGKYGCESDMALVELIHSYPEIDYVNIHIWPTNWGWSSRINPTEEDLER
                     SISKSQEYVDEHLPIALRLGKPIIIEEFGYPRDGYSFSKESTTEHRDAYYEFIFGQVE
                     QSRLSGGVIAGCNFWGWGGFAEPSPDRDYWQKGDPYTADPAQEPQGLFSLFHDDSTVE
                     IIRKYSTI"
BASE COUNT          472 a          427 c          393 g          421 t
ORIGIN      
        1 gggtgacaaa tggtgtatct acccgatgta tgactacgca catggtcaat gtgactccat
       61 agaaagaata acccattcca tttgcactct ggaatttgac gtacacagac ctctctacga
      121 ctggtttatc gagaacttgg agattttccc ttcccgccag tacgaatttg cccgcctcaa
      181 tctcacctat accgtaatga gcaagcgcag acttctcgaa ctggtacgca acaacattgt
      241 ggagggttgg gatgaccccc gcatgccaac aatatgcggc ctccgtagaa gaggctacac
      301 tcctgagagc atccgtctgt ttgctgataa agttggggta gccaaacgtg acaacgtcat
      361 agatctgtca cttctcgagt ggtgcctcag ggatgacctc aatcgccgtt ccaaccgcta
      421 tatggcagtg atcaacccca tcaaactggt tattgacaat tgggatgaag gcaaagtgga
      481 atattttgaa gctcccttga atccggaata tcctgacggt cccaaacgca gggtagcatt
      541 cacgaaagaa ttgtatatcg aaagcgaaga ctttatggag gaggctccaa agaagtacca
      601 ccgcctaaaa cctgacggtg aggtacgcct caagtattcc tatatcgtaa aatgtgtgga
      661 ctacattaaa aacgaagccg gagaagtagt tgaaattcat tgcacctacg accctgccag
      721 caaaccgggg gcaggtccat ggcgctccgt taaggggaca atccactggg cttcggttac
      781 ccattcggaa aagattaccg tcaacctttt cgacaaactt ttcaccgaag agcagatggg
      841 ctccattccc gaagggaagg actacagaga ttacatcaac cccgactctt tgatagtaaa
      901 agaggcattt gccgaacctg accttcttaa agatgactca ggtatggcag tccagtttga
      961 acgtatggga tactttatca aagatcccgt aaacgggggc tacaacaaag ccaccgggct
     1021 caaggactcc tttaaattgt agaatattta cggatgattt ccaccgtgga atcatcgtgg
     1081 aatagcgaaa acagaccctg cggctcctgc gcggggtctg ccgtataggg gtctcctttc
     1141 tgccaataat ctctatccgg tgagggttct gcaaagcctc cccaacccca gaaattgcag
     1201 cctgcgatga ctccacccga aagacgtgat tgttctacct gtccaaaaat gaactcataa
     1261 taggcatcac gatgctcggt agtgctctcc tttgagaagc tgtaaccatc ccgtggataa
     1321 ccaaattcct ctatgatgat tggcttacca agacgcaatg ctatcggcag atgctcatca
     1381 acgtactctt gtgatttgga gatggaacgc tccaaatcct cttctgtagg atttatacga
     1441 cttgaccacc cccaattggt tggccagata tggatgttga catagtcgat ctcgggatat
     1501 gaatgaatca gctctacaag agccatatcg ctttcacaac catatttacc ctcactgccg
     1561 gttgaaatca tgtgattggg gtcaatactc ctgatcaatt gtgcagtctc cttgatccac
     1621 tcggcaaaga gagaacgatt gtcattctcg cctgaggaga agggacgcgg ttcattgcat
     1681 atctgccatg agaatattgc aggatcctca ctg
//
LOCUS       DAOSIE010000311         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_61103_length_1705_cov_2.478182, whole genome shotgun sequence.
ACCESSION   DAOSIE010000311 DAOSIE010000000
VERSION     DAOSIE010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61103_length_1705_cov_2.478182"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1372)
                     /locus_tag="PLI69_07255"
     CDS             complement(2..1372)
                     /locus_tag="PLI69_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG42318.1"
                     /translation="MKFVDRTDEITRLKEALGREKSSLVVLYGRRRLGKSTLVKRVLS
                     DNDVYFLADRSEGQHQRSLLAKVVAQLFPDFDKLTYPDWESLFRAINYRTETHFTLCL
                     DEFPYLVEQSKELPSVLQKLIDEKQLKYNLVLCGSPQNMMYGLFFDSGAPLYGRADEI
                     MRLTPLRLPYIKEALNLDAINAVEEYAVWGGVPRYWELRENRASLNDALWQNIFSVNG
                     ILYEEPIKLFQDDVKDIVKTSTIMSYIGDGANRLSEIASRCNEPATNLSRPLKKLIDL
                     GFLEKEVPFGVDEKKAKKSLYKIADPFMAFYYKFVVPNRSFIELGRRLPIEQALNAHF
                     LEYVGMQWEKLCRDAVTGNLINGVIYGKAKRWWGSVRNEENKPEQFEFDVMAESLDKK
                     YLLVGECKWTTLENGKLLTVELIRKANLLPFARNYTIIPMLFLKNPPKDDIGNTLLPK
                     DIVKYS"
BASE COUNT          507 a          381 c          291 g          526 t
ORIGIN      
        1 ctcaactata cttcacaata tcctttggta atagggtatt accaatatca tccttagggg
       61 gatttttaag gaatagcatc ggtatgatgg tgtagttccg ggcaaatggt agaagattgg
      121 ctttgcggat cagttcaact gtgagtagtt ttccattttc aagagtagtc catttgcatt
      181 ctccgacaag caaatatttt ttatcgagcg attctgccat cacatcgaac tcaaattgtt
      241 ccggtttatt ttcctcattt cgcaccgaac cccaccaacg ttttgcctta ccatagatta
      301 cgccattaat aagatttcct gtcacggcat ccctgcatag cttctcccat tgcataccca
      361 catattctaa gaaatgggca ttaagggctt gttcgatagg tagccgacga ccaagttcta
      421 taaatgaacg attaggaaca acaaacttat agtagaatgc catgaatgga tcggcaatct
      481 tataaagact cttttttgcc tttttttcat caacaccgaa tggcacttct ttttccagga
      541 atccgagatc aataagtttc tttagaggac gagacaaatt ggtcgcaggc tcattgcatc
      601 gtgacgcaat ctctgaaagg cgatttgcgc cgtcaccgat atatgacatt attgttgaag
      661 tctttacaat atcctttaca tcatcctgaa ataactttat aggttcctcg tacagaattc
      721 catttactga aaaaatattt tgccacaagg cgtcattaag tgaagctctg ttttctctta
      781 attcccagta gcgcggcact cctccccaaa cagcatactc ctccacggcg tttatcgcat
      841 caagatttaa agcctcttta atataaggca agcggagagg tgtaagcctc attatctcat
      901 ctgcacggcc atatagaggt gcgccagagt cgaagaataa cccatacatc atattttgcg
      961 gggaaccgca aagaaccaga ttgtatttta attgcttctc atcaataagt ttctgcaata
     1021 cggacggcaa ttctttagat tgctccacaa ggtacggaaa ctcgtccagg cacaaagtga
     1081 aatgcgtctc ggttcgataa ttaatggctc gaaataaaga ctcccagtca ggatatgtta
     1141 gtttgtcgaa atccggaaat agttgtgcta ctacttttgc aagcaatgac ctctggtgtt
     1201 gtccttccga acggtcggcc aggaaatata cgtcattatc cgacagcacc ctttttacaa
     1261 gagttgactt gcccaagcgt ctgcgaccgt acagtacaac tagtgaggat ttctctcttc
     1321 caagagcttc cttaagcctt gtgatttcat ctgttctgtc aacaaatttc atgttttata
     1381 aattattatg aaacacaaac ataatgttta tattgcataa taaaaaattt atttccctct
     1441 ttttgcccaa attctcacaa tctcaacaaa acatttcggt ttaatattat gcataactaa
     1501 cataatgtac ttgaagcata attaacttgc tctgattata agcaactcaa atttgtgctg
     1561 aagctgttgt actaagaact ctatagactc aactccaaaa tttccaaata ctccttactc
     1621 catactcaga actcataact aacctacatt tcgcaacaca aaaaaacctg aaagtcataa
     1681 actttcaggt ttttttgtca agccc
//
LOCUS       DAOSIE010000312         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_61189_length_1703_cov_3.438107, whole genome shotgun sequence.
ACCESSION   DAOSIE010000312 DAOSIE010000000
VERSION     DAOSIE010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61189_length_1703_cov_3.438107"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /gene="dxr"
                     /locus_tag="PLI69_07260"
     CDS             complement(<1..896)
                     /gene="dxr"
                     /locus_tag="PLI69_07260"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOG42319.1"
                     /translation="MEQPKRSVAILGSTGSIGTQTLEVISHNSDLFEVDLLTANNNSR
                     LLIEQAIRFKPNNVVICNPSKYEEVKSALAPHYIKVFQGMDSVCDLVGSSTVDIVVTA
                     MVGFSGLQPTISAIKAGKVIALSNKETLVAAGEIIMSLAAEYGSPIIPVDSEHSAIFQ
                     ALQGERTNPEKLLLTASGGPFLHTPAKELYKVTPKEALRHPRWKMGSKVTLDSATLMN
                     KGLEVIEAHWLFNMDPERIEVVIHPQSIIHSMVQFEDGSVMAQLSSPDMRLPIQYALS
                     YPYRIPLDTPRISFGELAELTF"
     gene            complement(900..>1703)
                     /locus_tag="PLI69_07265"
     CDS             complement(900..>1703)
                     /locus_tag="PLI69_07265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG42320.1"
                     /translation="INDDSHKELFSFRTSKINIILVSILLLLAVMSGTYLLTSRTFIR
                     HTIPGYPSTESKRMAVENAIKIDSLERAVEMWAFQVANIQRIVTGKKPLTPDSLGIAR
                     ETPPLDGVLSDLYYRSDSLLREEVLRREQYNVSGQRQEIEQIEGLHFFTPVSGIITEA
                     YNKATGHPYIDVAAQTGTIVHSVLDGTVISAGWSDDTGYTLQIQHSHDIISVYKHNEQ
                     LLKSSGDKVKAGTPIAMVGNTGKLSTGSHLHFELWHKGEAVDPTLYIKF"
BASE COUNT          376 a          415 c          450 g          462 t
ORIGIN      
        1 aagaaggtta gttcggccag ctctccaaaa cttatccgag gagtatccaa agggatacgg
       61 taggggtagg agagggcata ttgaatgggt agccgcatat cgggtgaact gagctgggcc
      121 attacgcttc catcctcaaa ttgtaccatg gaatgaataa tgctctgcgg gtgaattacc
      181 acctctatcc tctccgggtc catattgaag agccagtgcg cctcaatcac ttccagcccc
      241 ttattcataa gagttgcgga gtcgagggtc accttggaac ccatcttcca gcgaggatgc
      301 ctgagggcct ccttaggtgt tactttatag agttctttag cgggagtatg gagaaaaggg
      361 cctccggatg cagtcagcag gagcttctcc gggttggttc tctccccttg cagcgcctgg
      421 aatatggcag agtgctcgga gtccaccgga atgatggggc tgccgtactc cgctgccaat
      481 gacataatga tctctccggc ggcgaccagc gtctctttgt tggagagtgc tattaccttt
      541 cccgccttta tggcggaaat tgtgggctgt aaaccgctga accccaccat tgcagtcaca
      601 acaatatcta cagtggaact cccgaccagg tcgcaaacgg aatccatacc ctggaatacc
      661 tttatgtagt gaggtgcgag tgcacttttt acctcctcat atttcgaggg attgcatatt
      721 accacattgt ttggcttgaa tctgatcgcc tgctctatca gcagcctgct attgttgttt
      781 gctgtaagca aatccacctc aaagaggtcg ctgttatgcg aaatcacttc gagggtttgt
      841 gtgccgatag atcctgtgga tccaagtatg gctactgatc ttttgggttg ctccatctgc
      901 taaaacttga tgtaaagagt ggggtctaca gcctctccct tgtgccagag ctcgaagtgg
      961 agatggctgc cggtgctgag tttccccgtg ttaccaacca tcgctatagg ggtgccggcc
     1021 ttgaccttat cgccactgct tttgagcagt tgctcgttgt gcttataaac ggagatgata
     1081 tcgtggctgt gctgtatctg gagggtgtat cccgtgtcat cgctccatcc tgcggaaatg
     1141 acggtaccat cgagtacgga gtgtactatt gtgccggtct gggcggcaac gtctatatag
     1201 gggtgcccgg ttgccttgtt atatgcttcg gtaattatac cgctcacagg ggtaaaaaag
     1261 tgaagtccct caatctgctc tatctcctgc ctttgcccgg agacattgta ctgctcgcgg
     1321 cgcagcacct cctctctcag gagagaatcg ctgcgataat agagatcact aagaactcca
     1381 tctagcggag gagtctccct cgctattccg agggagtcgg gggtaagagg cttctttcct
     1441 gtgacaatcc tttgaatatt tgccacctgg aatgcccaca tctccacggc tctctcgaga
     1501 gaatctatct ttatggcatt ttctaccgcc atgcgcttgc tttccgtaga gggatagccg
     1561 ggaatggtat gccttatgaa agtgcgtgaa gtcagcaggt aggtccctga cataactgcg
     1621 agcaatagta ggatagagac cagtatgatg ttgatttttg aggttctgaa cgagaataac
     1681 tccttgtggg agtcatcgtt gat
//
LOCUS       DAOSIE010000313         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_62228_length_1684_cov_3.114180, whole genome shotgun sequence.
ACCESSION   DAOSIE010000313 DAOSIE010000000
VERSION     DAOSIE010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62228_length_1684_cov_3.114180"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /gene="rho"
                     /locus_tag="PLI69_07270"
     CDS             <1..854
                     /gene="rho"
                     /locus_tag="PLI69_07270"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOG42321.1"
                     /translation="TPLFPDEKFNLTGKGHNNNFSIRIVDLFTPIGKGQRGLIVAQPK
                     TGKTVLLKDIANAIADNHPEVYMIVLLIDERPEEVTDMQRNVKAEVVASTFDEPAERH
                     VKVANMVLEKAKRLVECGHDVVILLDSITRLARAFNTVQPASGKVLSGGVDANALHRP
                     KRFFGAARNIENGGSLTILATALTETGSKMDEVIFEEFKGTGNLELQLDRKLSNKRIF
                     PAVDVTLSSTRRDDLLYNPDYLNRIWILRNHLADMNSVEAMEFMKVRLQRCANNEEFL
                     VTMANGE"
     gene            870..>1684
                     /locus_tag="PLI69_07275"
     CDS             870..>1684
                     /locus_tag="PLI69_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOG42322.1"
                     /translation="MSAVSLLILYLLLAISVSFLCSIVEAVLLSTPISFITMKEDEGL
                     KGAALFKKYKTDIDRPISAILTLNTIAHTVGAAGVGAQAAIIFNNSYFGLVSAITTFL
                     ILVFSEIIPKTLGASYWKRMTNFTAYTIRLMIFVTYPLVILFESLSKLISPKEQEVTI
                     SREEVSAMVNVGEEEGVFEQSENKIIQNLIKLDSIKAYDAMTPRVVSAIASENMTLKE
                     FYKDSSYLHHSRIPVYADSPEFITGYILRREALEYLADDKFEVKLGDIRRDIP"
BASE COUNT          478 a          386 c          374 g          446 t
ORIGIN      
        1 tgacacctct tttccccgat gaaaagttta accttaccgg aaagggacac aacaacaact
       61 tctccattag gatagtggac ctatttacac ctataggaaa gggacagaga ggacttatcg
      121 tggctcaacc caagacggga aaaactgtac tcctaaagga tattgccaat gctattgccg
      181 acaatcatcc cgaagtctat atgatagtac ttctgattga cgaacgtcct gaagaggtga
      241 cagatatgca gcgcaacgtt aaagcggagg tagtggcatc tacgtttgac gaacctgctg
      301 agagacatgt aaaagtggct aatatggtgc ttgaaaaggc aaaacgtctg gttgagtgcg
      361 gtcacgatgt tgttatccta ctcgactcca ttacccgtct tgccagggcc ttcaatacgg
      421 tacaacctgc atccgggaag gtgctttccg gtggtgttga tgccaatgct ctccatagac
      481 ctaaacgctt ctttggtgcc gcccgcaata tagagaatgg cggatcgctc accatccttg
      541 ctaccgctct gacggagaca gggtctaaga tggacgaagt aattttcgaa gagtttaaag
      601 gcacaggcaa tctggagttg cagctcgata ggaaactctc caacaaacgc atcttccctg
      661 cagtagatgt gaccctctcc tctacccgca gagatgacct tctttacaat cccgattatc
      721 tcaaccgcat ctggattctc cgtaaccatc ttgcagatat gaactcggtt gaagctatgg
      781 aatttatgaa ggtaagactg caaagatgtg ccaataacga agaattcctg gttacaatgg
      841 caaatggaga ataattatat acctaatata tgagtgccgt atcacttcta atcctctact
      901 tgcttctggc tattagcgtg tcgttcctct gttcaatagt ggaagctgtc ctgctgtcta
      961 ctccaatatc tttcataaca atgaaagagg atgaaggctt gaaaggtgcc gctctattta
     1021 agaagtataa gacagatatt gacaggccca tttctgctat cctaactctt aacaccattg
     1081 cacataccgt aggtgccgca ggagtaggag cacaagcagc tataatattc aataattcct
     1141 atttcggact ggtctctgcg attacaacgt ttttgatatt ggtattctca gaaattatcc
     1201 ccaagaccct cggtgcctcc tattggaagc gcatgaccaa ctttacggct tacacgatac
     1261 gtttgatgat tttcgtcacc tatccgctgg taatcctctt cgaaagcctt tctaagctta
     1321 tctctcctaa ggagcaggag gtgacgataa gtcgtgagga ggtttcagca atggtgaatg
     1381 tcggtgagga ggagggtgtt ttcgagcaga gtgagaacaa aattatccaa aacctcataa
     1441 aacttgacag tattaaagcc tatgatgcca tgactccgag agtggtctct gcaattgctt
     1501 cagagaacat gacgctaaag gagttttaca aagactcttc ctatctgcac cactccagga
     1561 taccggtcta tgccgactca cccgagttta ttaccggtta catactcaga agagaggccc
     1621 tcgaatacct tgctgatgac aagtttgaag taaaattggg agacatcaga agggatatcc
     1681 ccta
//
LOCUS       DAOSIE010000314         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_62403_length_1681_cov_2.321648, whole genome shotgun sequence.
ACCESSION   DAOSIE010000314 DAOSIE010000000
VERSION     DAOSIE010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62403_length_1681_cov_2.321648"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="PLI69_07280"
     CDS             <1..169
                     /locus_tag="PLI69_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOG42323.1"
                     /translation="KLLANVVAGKVFERDTEMEWQCRNCGYIFKGKKAPNECPACKHA
                     QSYFEVKKNNY"
     gene            191..>447
                     /locus_tag="PLI69_07285"
     CDS             191..>447
                     /locus_tag="PLI69_07285"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG42324.1"
                     /translation="MKIKTADREGYALITGTWSGMGREYARQLYALGHNLIVIDINQK
                     FADEVCEELYGLETTPSELRPDVIAIGVDLALVGAAAIIAE"
     assembly_gap    448..457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <458..1003
                     /locus_tag="PLI69_07290"
     CDS             <458..1003
                     /locus_tag="PLI69_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG42325.1"
                     /translation="VDILINNAGVLPYGDFTEYSQEEISRVIVLHNLTLVQLCNLFAS
                     GMKERGYGYILNISSLCSWMPYPVLSVYSATKRFIKSFSRSLRVELMGSGVSVTTAYF
                     GAVSTNLFPLQESMRKRAFRLGIMISAEEAARKALNATFRGRANVMPGLINRIFLPIL
                     PIVPKRMLHFFHKKYGDKIKF"
     assembly_gap    1102..1111
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1112..>1681
                     /locus_tag="PLI69_07295"
     CDS             <1112..>1681
                     /locus_tag="PLI69_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HOG42326.1"
                     /translation="YISDAVGPVFSYFGLPGSAALAYLSGYFINVYSAIAVMSTLALD
                     ARELTILSTMVLCSHSMILETAVLKKTGVSGLRMIIIRTLSAIILGWFLNLILPAGSG
                     EVVTEAVAQEQLTFWLTLRNWLWATFKLVLLITVIIYAFNIIQRLLSAFGIIAIISRF
                     LKPVMLFFGLPPKTAFLWIVANIIGLAYGA"
BASE COUNT          417 a          361 c          396 g          487 t
ORIGIN      
        1 gaagctctta gccaacgttg tggcaggtaa ggtatttgag agagatacgg agatggagtg
       61 gcagtgccgc aattgcggat atatattcaa gggaaagaag gcaccaaatg agtgtcctgc
      121 ttgcaaacat gcccaatcct atttcgaggt taagaagaac aattactaat tttttaatac
      181 ctttgcggtc atgaaaataa agaccgctga cagagaaggt tatgccctaa ttaccggtac
      241 atggtccgga atggggagag aatatgcccg acagctctac gctctcgggc ataatttgat
      301 tgtgatagac ataaatcaga aatttgccga tgaggtttgt gaggagctat atggccttga
      361 gacgactcct tcagagctac gccccgatgt gattgcaatt ggagttgatc tggctttagt
      421 aggcgctgct gccatcatag ctgaaaannn nnnnnnngta gatatcctga tcaacaatgc
      481 cggtgtgttg ccttatgggg acttcaccga atattcgcag gaggaaattt ccagggttat
      541 tgtccttcac aatctcacat tggtgcaact ttgtaacctc tttgcctccg gtatgaagga
      601 gcggggatac ggctacatac tcaatatctc ttcgctttgt agttggatgc cttaccctgt
      661 gctctctgtc tattccgcca caaagcgttt tataaaatcc ttttcacgtt ccctaagggt
      721 cgagctgatg ggtagcggag tctcggtgac tactgcctat ttcggcgctg tttcaaccaa
      781 tctgtttcct ttgcaggaga gcatgcgcaa gcgggcattc cgtctgggta ttatgatttc
      841 tgctgaagaa gctgcccgta aagccctcaa tgctacattc cggggtagag ccaatgtcat
      901 gcccggtctc atcaaccgta tatttctgcc gatattgccc attgttccaa aacgtatgct
      961 tcatttcttc cataagaaat atggcgacaa gatcaaattt taatatgaaa tcggagacca
     1021 agcagaagat tatttcctct attaaagagg taattcccac aactaccaag acctgcatct
     1081 ggattttgaa gattacggtc gnnnnnnnnn nctatatctc tgatgccgta ggtcccgtat
     1141 ttagttattt cggcttaccc gggtcggctg ccttagccta tttgtcgggc tatttcatca
     1201 atgtatattc agcgatagcg gttatgagta cccttgcact ggatgcacgt gaacttacca
     1261 tcctcagtac gatggtgctt tgttcgcact ctatgatact ggagacggca gtactcaaaa
     1321 agacaggcgt ttcggggctg aggatgatta ttataaggac tttgagcgca ataattctcg
     1381 gttggtttct caatctaatc cttccggccg gtagcggaga agtggtaaca gaggcagtag
     1441 cgcaagagca gctcactttt tggctgactc tgcgcaactg gctatgggct acatttaagt
     1501 tggtgttgtt gataacagtg ataatctatg ccttcaatat tatacagcga cttctttcgg
     1561 cgtttggtat tattgcgatc atatcgagat ttctaaaacc ggtcatgctc tttttcggac
     1621 ttccgcccaa gacagcattt ctatggatag ttgccaatat cataggtctg gcctatggcg
     1681 c
//
LOCUS       DAOSIE010000315         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_63297_length_1665_cov_4.344099, whole genome shotgun sequence.
ACCESSION   DAOSIE010000315 DAOSIE010000000
VERSION     DAOSIE010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63297_length_1665_cov_4.344099"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..288)
                     /gene="rpsN"
                     /locus_tag="PLI69_07300"
     CDS             complement(19..288)
                     /gene="rpsN"
                     /locus_tag="PLI69_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOG42327.1"
                     /translation="MAKESMKAREVKRAKLVAKYAEKRKALKEAGDWVALDKLPKNSN
                     PCRLHNRCSITGRPKGYMRQFGISRIQFREMASKGLIPGVKKASW"
     gene            complement(296..904)
                     /gene="rplE"
                     /locus_tag="PLI69_07305"
     CDS             complement(296..904)
                     /gene="rplE"
                     /locus_tag="PLI69_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOG42328.1"
                     /translation="MAKEKKTQVQAGPTVDQVNYVPSLKTKYKEEIVGKLMKEFSYST
                     VMQVPRLVKITINQGIGAATQDRKLVDVAQQELSLIAGQKAVQTASRKDISNFKLRRG
                     MPIGVKVTLRSTKMYEFLERLIAVSLPRIRDFKGISENFDGRGNYTLGIKEQIIFPEI
                     DIDKIVKVMGMEITFVTTAKKDEEAYALLREFGLPFKNIKHN"
     gene            complement(904..1224)
                     /gene="rplX"
                     /locus_tag="PLI69_07310"
     CDS             complement(904..1224)
                     /gene="rplX"
                     /locus_tag="PLI69_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOG42329.1"
                     /translation="MTKLHIKKGDMVYVNAGNDKGKTGKVLEVITKKNRAIVEGVNMV
                     SKHTKPNSKHPQGGIVKREAGVHISNLQVVDPVKGGATKIGRRLNEDGKIVRYAKKSG
                     EEIK"
     gene            complement(1240..1605)
                     /gene="rplN"
                     /locus_tag="PLI69_07315"
     CDS             complement(1240..1605)
                     /gene="rplN"
                     /locus_tag="PLI69_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOG42330.1"
                     /translation="MIQQESRLMVADNSGAKEVLCIRVLGGTRRRYAGVGDKIVVAVK
                     SAIPGGNTKKGAVSNAVIVRTKKEIRRADGSYIRFDDNACVLLNKSDEVIGTRIFGPV
                     ARELRENYMTIVSLAPEVL"
BASE COUNT          390 a          421 c          352 g          502 t
ORIGIN      
        1 ggtgcccgaa cccgcggttt accaactcgc cttcttcact cccgggatca ggccttttga
       61 agccatttca cggaactgaa tacggcttat gccgaactga cgcatataac cctttggacg
      121 accggtgatg gaacaacggt tgtggagtcg gcaggggttg gagttctttg gaagcttgtc
      181 aagggctacc cagtctccgg cttccttcaa agccttacgt ttctcagcat acttagctac
      241 taatttagcg cgttttactt cgcgagcctt cattgattct tttgccatat cctatttagt
      301 tgtgcttaat gttcttaaaa ggtaaaccaa attcacggag gagcgcataa gcctcttcgt
      361 cttttttagc ggtagtaaca aatgtaatct ccattcccat caccttcaca atcttatcga
      421 tgtcaatctc ggggaaaata atctgctcct tgatgccgag ggtatagttg ccacgaccat
      481 caaaattctc ggagataccc ttgaagtccc tgatacgggg aagggataca gctatcagcc
      541 tctcgaggaa ctcatacatc ttggtggagc gaagagtaac ttttacgccg ataggcatgc
      601 ctctacggag cttaaagttg gagatgtcct ttcttgaagc agtctgaact gctttttgac
      661 ctgcaatcaa agagagctcc tgctgtgcta catcaacgag cttcctatcc tgggtggctg
      721 cgcctatacc ctgattgatg gtgatcttta caagtctggg aacctgcatt acggtggagt
      781 aggaaaattc cttcatcagt ttacctacta tttcctcttt gtacttcgtc ttcaaagaag
      841 gaacataatt gacctggtcc actgtaggac ctgcctgcac ctgggttttc ttctcttttg
      901 ccattatttg atctcctctc ctgatttttt ggcatagcga acaatcttgc cgtcttcatt
      961 gagacgacga ccgatcttgg tggcaccacc cttaacggga tcaacaacct gtaagttgga
     1021 aatgtgcaca ccggcctctc gtttaacaat acccccttga gggtgttttg aattgggttt
     1081 ggtatgtttg cttaccatgt tgacaccttc aacaatggcg cgattcttct ttgtaatcac
     1141 ctcgagaacc ttaccggtct tgcctttatc gttcccggcg tttacatata ccatgtcgcc
     1201 tttcttgata tgtaactttg tcataattca aatcctcctt taaagtacct ccggggcaag
     1261 tgagacgatt gtcatatagt tttcacgcag ctcacgagct acgggaccaa aaatacgtgt
     1321 accgataacc tcatcagact tgttgagaag aacgcatgcg ttgtcatcaa aacgtatgta
     1381 agaaccgtca gcacgacgaa tctctttctt tgtacgcact atcacggcgt tagaaactgc
     1441 acctttcttg gtgtttccac cagggatggc actctttacg gcaacaacga tcttgtcgcc
     1501 gactccggca taacgacggc gtgtaccgcc caaaacacgg atacagagga cttcctttgc
     1561 accgctgttg tctgccacca tcaaacgtga ttcttgttgt atcatgttat ttcacttttt
     1621 ctactatttc tactaatctc cagcgtttgg tcttgctcag aggac
//
LOCUS       DAOSIE010000316         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_64418_length_1643_cov_3.146096, whole genome shotgun sequence.
ACCESSION   DAOSIE010000316 DAOSIE010000000
VERSION     DAOSIE010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64418_length_1643_cov_3.146096"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLI69_07320"
     CDS             complement(<1..845)
                     /locus_tag="PLI69_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42331.1"
                     /translation="MKRVLIMLAGAITILGNVGCDSGTKFKSIDGIEKYRTYYNESLK
                     ELSSEAYHGRSNYGDGNIKAALYIIGELQKMGIGPMVDEIGAEPIQATPPFKGTASPC
                     GPMRFEGTPDDQMAYLQHYCLPLNVMRGAMQVIIDGNELVPAVDYVAKEFSPACKGEV
                     GLAYPGVDKYTSVKELADHINSGIYKKKFVVIEWGPYTETIGTNPFDRYREFMDMLQA
                     DKVAGIIYKMESKLPYFKARSYYVTPVPAFCIWNGLTEGAKKIKVNMDCELIECHDAH
                     NVLAS"
     gene            complement(887..1636)
                     /locus_tag="PLI69_07325"
     CDS             complement(887..1636)
                     /locus_tag="PLI69_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG42332.1"
                     /translation="MKPYALIFAAGLGTRLKPLTDTMPKALVPVGGVPLLERVILRLK
                     QAGYRHIVINVHHFAEQIIDFIEQKESFGISIAISDERDLLRETGGGIKHAAHLLKSD
                     HFLVHNVDIISNLDLSLFEEKYLETEKEHNNSERPLLATILVSKRKTQRFFLFDSRNN
                     LVGWTNIATGEVRSPFPGLDPGKCRKLAFAGIHNISKRVFPLMEKWPERFPIVDFYIA
                     NCGKYLIKGIEVPGLSIRDVGKISDLNSLKY"
BASE COUNT          431 a          372 c          371 g          469 t
ORIGIN      
        1 atcgaggcga gaacattatg agcgtcgtgg cactcaatca actcgcaatc catgtttact
       61 tttattttct ttgctccctc ggtaagtccg ttccatatgc aaaatgcggg aacgggtgtt
      121 acataatagg accgtgcctt gaaataaggg agtttggact ccattttgta gataatcccg
      181 gctaccttgt ccgcctgtag catatccata aattcacggt atctgtcaaa gggatttgtg
      241 cctattgttt cagtataggg tccccattct atcactacga atttcttttt gtaaataccg
      301 ctgttgatat ggtcagcgag ttccttaacg gaagtatatt tgtctacacc gggatatgca
      361 aggccaactt cgcctttgca ggcgggagag aattcctttg caacatagtc aacggccggg
      421 acaagttcgt tgccatcaat gatgacttgc attgcaccac gcataacatt cagaggtaag
      481 cagtaatgtt gtaaatatgc catttggtca tcaggggtgc cttcaaagcg cattggacca
      541 caaggtgaag cggtaccctt gaagggaggt gtagcctgta tgggttctgc accaatctca
      601 tcaaccatag ggccgatacc catcttctgc aactcgccta ttatgtataa ggctgccttg
      661 atgttgccat ctccatagtt gctccgtccg tgataggctt cacttgagag ttcttttagt
      721 gattcgttgt aataggtgcg gtatttttcg ataccatcga tgctcttaaa cttggttccg
      781 ctgtcacaac cgacattgcc aagaattgta attgctcctg caagcataat caatacacgt
      841 ttcatagata gctgtttatt gcgttattag tattaaaaag ataaagttaa tattttagcg
      901 agttcagatc cgatatctta ccaacatccc ggattgaaag tcccggcact tcaatgcctt
      961 taataagata cttgccacag ttggctatat aaaaatccac gatgggaaag cgttcaggcc
     1021 atttttccat aagtggaaaa actctcttcg atatgttgtg gatccccgcg aaggcaagtt
     1081 tacgacattt gccgggatca agtcccggaa acggagaacg aacctcaccg gtggcgatat
     1141 tggtccagcc gaccagattg ttacgactgt caaagagaaa aaaacgctgt gtcttacgct
     1201 tacttaccag aatagttgcc agaagaggcc tttcagaatt gttatgctct ttttccgttt
     1261 ccagatattt ttcctcgaag agggaaaggt ccagattgga tatgatatcg acattgtgaa
     1321 ccaggaaatg gtcggacttc aacaggtggg cagcatgctt tataccgccg ccggtctcgc
     1381 gcagcagatc tctctcatcc gatattgcaa tcgaaatccc aaaagactct ttttgctcaa
     1441 taaaatcaat gatttgctct gcaaaatggt ggacattgat cacaatatgg cggtacccgg
     1501 cctgcttcag gcgaaggatt accctttcaa gcagtgggac acccccgaca ggcacaagtg
     1561 ctttgggcat ggtgtctgtc agcggtttga gtcttgttcc cagacctgcg gcgaatatga
     1621 gagcgtaagg cttcatctca aaa
//
LOCUS       DAOSIE010000317         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_65965_length_1616_cov_3.139654, whole genome shotgun sequence.
ACCESSION   DAOSIE010000317 DAOSIE010000000
VERSION     DAOSIE010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65965_length_1616_cov_3.139654"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLI69_07330"
     CDS             complement(<1..741)
                     /locus_tag="PLI69_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG42333.1"
                     /translation="MIKKTIIDHFEQAVAQFPDNAYLLESKTGTFEPTTYKQTRDAAY
                     KFGAGLVLEGVKPKDNVAILAEGCNMWIISELGLFYAGGISVPLSIKLEESNDLLFRL
                     IHGDVKYIITSSRQLNKIRLIRDKLPLVEKVIVLDPAESYQAGEIAAEDLMKRGAKYL
                     ETNMDEFLKIGQAIQNEDIATITYTSGTTADPKGVVLTHRNYTANVEQSLTCINIDEN
                     VKMLIILPLDHCFTLWYGGYFGSSGHHGV"
     gene            complement(786..>1616)
                     /locus_tag="PLI69_07335"
     CDS             complement(786..>1616)
                     /locus_tag="PLI69_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOG42334.1"
                     /translation="LIAAIDLRAELVERESNRIVVEFSWKNGSSFSGVLEICGKIPIP
                     PYLKRDPQPLDRFRYQTCYARIEGSVAAPTAGLHFTGEVLEKISEKGINQGYISLHVG
                     AGTFLPVKSEYISEHNMHSEPFSITLEMIKSLRNYTSKHIIAVGTTSTRCLESLYFLG
                     VQVIEKKAPTYVSQWEPYRDEGYDYTLEEALDALIGYLEENSLHFLLSRTEIIILPGY
                     RFRVIDHLITNFHQPKSTLLLLISAFVGPSWRDIYKFALENEFRFLSYGDSSLLEIRK
                     "
BASE COUNT          447 a          347 c          360 g          462 t
ORIGIN      
        1 tacgccatga tggccagagg agccaaaata gccaccgtac cacagggtaa aacagtggtc
       61 caaaggcaat ataatcagca tcttgacatt ctcatcgata tttatgcaag taagcgactg
      121 ttccacgttt gcagtatagt tgcgatgcgt aagcaccaca cctttcggat ctgcggtggt
      181 accggaagta taggtaatgg tagcaatatc ttcgttttgt atggcttgtc caatcttcag
      241 gaattcatcc atattggtct ccaggtattt ggctccgcgc ttcataaggt cctcagcagc
      301 aatctcaccc gcctgatagc tctctgccgg atcgaggacg attactttct ccacaagcgg
      361 gagtttgtcc ctgatcagac ggatcttatt caattgtctg gaactggtga tgatatattt
      421 aacatcgcca tgaatcaatc tgaagagcaa atcgttggac tcttcgagct taatggagag
      481 aggtacactt atgcctccgg cataaaacaa acccaactca ctgataatcc acatattgca
      541 accttctgcg aggatagcga cattatcttt gggcttgaca ccttcaagta ccagaccggc
      601 tccgaattta tatgcagcat ctcttgtctg cttataggta gtaggttcaa aagtacctgt
      661 tttgctctca agcaggtaag cattgtcggg gaactgcgca acagcctgct caaaatggtc
      721 aataatggtt tttttgatca taatattata ggttttagtc ttaatcaagt tacaaatata
      781 attttttatt ttctaatttc caacaaggaa gagtcgccat aactcaggaa acgaaactca
      841 ttttcaagtg caaatttata gatgtctctc caggatgggc cgacgaaagc agagattaac
      901 aacagtagtg tacttttggg ctgatgaaag ttggtaataa ggtgatcaat cactctaaac
      961 cggtatcccg gaagtattat gatctctgtt cgggagagta aaaagtggag tgaattttct
     1021 tcgagatagc ctataagagc atccaaagcc tcttcaagtg tataatcgta gccctcatca
     1081 cgatatggtt cccattgtga aacgtatgta ggagcttttt tctctatgac ctgaactccg
     1141 agaaaataga gcgactcaag gcagcgggtg gaagtagtac ctacggcaat tatatgcttt
     1201 gaagtgtaat tcctcaggga tttgatcatt tccagggtta ttgaaaaggg ctcgctgtgc
     1261 atattgtgtt cggatatata ctcgcttttt accggaagaa aggtgcccgc acccacgtga
     1321 agggagatat agccttgatt tatccccttt tcggagattt tttcaagtac ttccccggta
     1381 aagtggagtc ctgccgtagg tgcagcgacc gaaccctcta tacgggcata gcaggtctgg
     1441 tagcgaaatc tgtccagtgg ctgaggatcg cgtttgagat agggaggaat agggatttta
     1501 ccgcatattt ccagtactcc ggaaaatgaa gagccatttt tccaggaaaa ttccacaaca
     1561 atcctgttac tttctctctc gaccagttca gccctcagat ctattgcagc aattag
//
LOCUS       DAOSIE010000318         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_67585_length_1589_cov_2.107562, whole genome shotgun sequence.
ACCESSION   DAOSIE010000318 DAOSIE010000000
VERSION     DAOSIE010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67585_length_1589_cov_2.107562"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..902
                     /locus_tag="PLI69_07340"
     CDS             24..902
                     /locus_tag="PLI69_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOG42335.1"
                     /translation="MKQVQEKFISPSNGWMMLFVSIIGMAVCIVVPAIFLSKGGSDWW
                     GLLFVVSTIFFFLLSGLTIVNPNESKVVLFFGKYVGTIVDNGFFFVNPFTKRTTVSLR
                     ARNLNADPIKVNDKMGNPIMIGIVLVWRVKDTFKASFEVDNYVKFVDVQSEAAIRNLA
                     GSYPYDNLEDEEAAITLRSGGEEVNDLLEKSLQDRLNIAGIEVMEARISYLAYAPEIA
                     SAMLQRQQATAIVAARHKIVEGAVSMVQMALQQLSDKEIIELDEEKKAAMVSNLMVVL
                     TSDRGATPIVNAGSLY"
     gene            917..1105
                     /locus_tag="PLI69_07345"
     CDS             917..1105
                     /locus_tag="PLI69_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42336.1"
                     /translation="MAKEKKPFVLRIDSETLASLEKWAADEFRSVNGQLEYIITEALR
                     KSKRLKTAKNEKAQPNEF"
     gene            1095..>1589
                     /locus_tag="PLI69_07350"
     CDS             1095..>1589
                     /locus_tag="PLI69_07350"
                     /inference="COORDINATES: protein motif:HMM:NF027689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase N-terminal
                     domain-containing protein"
                     /protein_id="HOG42337.1"
                     /translation="MNFKKIFPILIVALLAFSCNGSNSHIDIEDKGNERISGYATYTD
                     GTPIVGMVVSDGFSCVITDKEGHYSIESPSSNTFYIYASIPADCKIEENPDGNPDFYH
                     KYTVERSIYDFNFERIEIENDFVLYALADPQCANEKHIARFESETVADIKETQKSEEL
                     PPYGI"
BASE COUNT          448 a          354 c          379 g          408 t
ORIGIN      
        1 gggcggctga attttttaaa gaaatgaaac aagttcagga aaaattcatc tctccttcga
       61 atggctggat gatgttgttc gtctctatta ttggcatggc cgtctgcata gtggtgcctg
      121 ctatattcct cagcaaaggt ggaagtgatt ggtggggatt gcttttcgtt gtttccacga
      181 tctttttctt tcttctatcg ggtttgacca ttgtaaatcc gaatgagagc aaagtggtac
      241 tctttttcgg caaatatgtc gggacaatcg tggacaacgg attctttttt gtcaatcctt
      301 ttacaaaaag gactaccgtt tccctcaggg cacgcaatct caatgccgat cccattaagg
      361 tcaatgataa gatgggcaac ccgatcatga tcggcatcgt actggtatgg agagtcaagg
      421 acacatttaa ggcctctttc gaggttgaca attatgtgaa atttgtggat gtacagagcg
      481 aggccgcaat ccgtaatctt gcaggttcct atccttacga caatctcgag gatgaagaag
      541 ccgccatcac tttacgcagc gggggcgaag aagttaacga tctgctcgaa aaatctcttc
      601 aggatcgcct gaacattgca ggcatcgaag tcatggaggc gcgcatcagt tatcttgcgt
      661 atgctccgga aattgccagc gcaatgcttc agcgtcagca ggcaacagcc attgttgcag
      721 cacgccacaa aatcgtggaa ggtgcagtga gtatggttca gatggcactc cagcaactct
      781 ccgataaaga aatcatcgag ctggatgagg agaaaaaggc tgcaatggtg agcaatctga
      841 tggtagttct tacttcggat agaggcgcga ctcctatagt caatgccggc tcactttact
      901 aataaaggac aatgttatgg ccaaagagaa aaaaccattt gtcctcagga tagattcgga
      961 aacattggcc tccctcgaaa agtgggcggc cgatgaattc cgtagtgtca atggccagct
     1021 ggaatatatc atcaccgaag ccctccgcaa gtcaaaacgg ctgaaaacgg ccaaaaatga
     1081 gaaagcgcag cccaatgaat tttaaaaaga tattccctat actcatagtt gcgctgctgg
     1141 cattctcatg caatgggagt aattctcaca tagatataga ggataaaggt aatgaaagaa
     1201 tttccggtta tgccacttat acggatggca ctcccatcgt cggaatggtc gtaagcgacg
     1261 gctttagctg cgtcattact gataaagagg gtcattactc cattgaatca ccctcatcca
     1321 acaccttcta tatctatgca tccatccccg cagactgcaa gatcgaagaa aatcccgatg
     1381 gaaatcccga tttttaccac aaatacacgg tggagcgcag catctatgac ttcaactttg
     1441 agcgcatcga aatagagaac gactttgtgc tctatgctct tgccgatccg cagtgcgcca
     1501 atgagaagca tattgcccgt tttgagtcgg agacagttgc ggatattaaa gagacgcaaa
     1561 agagtgaaga gctgccccct tacggtatt
//
LOCUS       DAOSIE010000319         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_69435_length_1558_cov_3.598802, whole genome shotgun sequence.
ACCESSION   DAOSIE010000319 DAOSIE010000000
VERSION     DAOSIE010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69435_length_1558_cov_3.598802"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1558
                     /locus_tag="PLI69_07355"
     CDS             <1..>1558
                     /locus_tag="PLI69_07355"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOG42338.1"
                     /translation="VAYGTSTKASFTGSASMVKSEEIQKKITTNVTSALAGTSPGVQI
                     ITSSGDPTSNAATIRIRGVGSMSASNNPLIVVDGAPFAGAISDINPNDVESMSVLKDA
                     SAAAIYGNRGANGVILITTKKGVGDAQIKFDARFGSNSRLIPKYDLIDDPAQYYETYY
                     KLMFNQQYYAGYTEAESYAHADKYLFDQNNGGLGYQVFTVPAGQKFVGTNFKMNPNAT
                     LGYTDGEYYYTPDNWYDEVFHASFRQEYNLSVSGSGERFNYYASGGFLDDGGVISNSQ
                     YKRYTGRINADYQVKEWFKLVSSMMFTHSDSETNPYTGSWGSSGNVFYIANNVGPIYP
                     LYVRNADGSKKLDNGRLVYDSNNTNFIRPSFVGNAVRDNEYDSSRYFADVFVGKAGAI
                     ITPFKGFTLTANLNLLSDNTRQNNLYSIFGSGASTDGMTYVAHSRTWDINQQYLANYK
                     TDFNGSKSNLDVLLGYEQQNRKMQTIWGQNDHLFNPLVGELANADGTSGKQVSSYTNN
                     YMAQGFFARAQ"
BASE COUNT          421 a          385 c          346 g          406 t
ORIGIN      
        1 agttgcctac ggtacatcca ccaaggcatc ttttacagga tccgcttcga tggtaaagag
       61 tgaggaaatc cagaagaaga ttaccaccaa cgttaccagc gctctcgcag gtacatctcc
      121 cggtgtgcag atcatcactt ccagcggtga ccctacctct aatgcagcaa caatccgcat
      181 ccgtggtgtc ggctcaatga gtgcgagcaa caaccccctc atagttgtcg atggagcccc
      241 gtttgcagga gctatctctg acatcaaccc taacgacgtt gagtcaatgt cagttctaaa
      301 agacgcctct gcggccgcca tttatggtaa ccgcggtgca aacggtgtaa tcctcatcac
      361 cactaagaaa ggtgtagggg atgctcaaat taaatttgat gcccgtttcg gttcaaactc
      421 tcgtcttatc cctaagtatg atctgatcga tgatcctgct cagtattatg agacttatta
      481 caaactgatg tttaaccagc agtactatgc cggttatact gaggcagaga gttacgctca
      541 cgctgacaaa tatctgtttg accagaacaa cggaggtctc ggttatcaag tgtttaccgt
      601 tcctgcaggt caaaagttcg tcggcaccaa cttcaagatg aaccctaatg cgactctcgg
      661 ttacacggac ggtgagtatt actatactcc cgataactgg tatgatgaag tattccacgc
      721 atccttccgc caggagtata acctttccgt gagcggttca ggagagagat ttaactacta
      781 tgcgagtggt ggtttcctcg atgacggcgg tgttatcagt aattcacaat acaaacgtta
      841 cacaggccgt atcaatgctg actaccaggt gaaggagtgg ttcaagcttg taagcagcat
      901 gatgtttaca cactctgatt cagagaccaa cccttatacc gggtcatggg gctcttcagg
      961 taacgtattt tacattgcaa acaatgtagg tccgatttat cccctttatg ttcgcaatgc
     1021 cgacggatca aaaaaattgg acaacggtcg tttggtttat gactccaata ataccaattt
     1081 catccgtcct tcgtttgtgg gtaacgctgt ccgtgacaac gagtatgact cctcaagata
     1141 ctttgccgat gtattcgttg gtaaagctgg tgcgatcatc actccattca agggtttcac
     1201 ccttactgcc aacctcaacc ttttgagcga caatacccgt cagaacaacc tttacagtat
     1261 attcggtagt ggagcatcga ctgatggtat gacatatgtg gcccactcaa ggacatggga
     1321 tatcaaccag cagtatcttg ctaattataa gactgacttc aacggatcca agagcaacct
     1381 cgacgttctt ttgggctatg agcagcaaaa ccgcaaaatg cagactatat ggggtcagaa
     1441 cgaccacctc ttcaaccctc tggtaggaga attagccaat gctgatggta cctcgggcaa
     1501 gcaagtgtca tcatacacca acaactacat ggctcagggt ttctttgcac gtgcacag
//
LOCUS       DAOSIE010000320         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_69650_length_1555_cov_3.340000, whole genome shotgun sequence.
ACCESSION   DAOSIE010000320 DAOSIE010000000
VERSION     DAOSIE010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69650_length_1555_cov_3.340000"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLI69_07360"
     CDS             complement(<1..393)
                     /locus_tag="PLI69_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HOG42339.1"
                     /translation="MSTKQTTKVSNSTLFQEVAEMVASGNRVEIVGKGSSMFPLIRGG
                     KDKIVLRKVDNFSIERGNILLVKLENGNYVLHRVKKHDTRTLILRGDGNLRNVETCKA
                     EDVIAEAVEVIRGKKRIAKGSCSWKMYSR"
     gene            complement(410..1261)
                     /locus_tag="PLI69_07365"
     CDS             complement(410..1261)
                     /locus_tag="PLI69_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42340.1"
                     /translation="MAIETPSPEVTARLLPNFSPFRVTPPLGEATSPDKSAPLLIFKG
                     NKKLEKPEWEPSEEVCLDGVMFNIYHREGNMWVAIERDGVEYIMFVSSDKSSYQSNLS
                     LTKPGENFFLIALLRIAFLMAVAPYRTLKFHASVIEKEGKALIFLGESGTGKSTHSGL
                     WLKYVPGSSLLNDDEPIVRIMDDGEVVVFGAPWSGSTPCYRNVSAKVAALVQLEQHSE
                     NILTGPLGLEAYAQLFQLTALLQSDQTHRQYVISTVLDVAEKVPLYRLRNRPDREAVA
                     LSASILY"
     gene            complement(1297..>1555)
                     /locus_tag="PLI69_07370"
     CDS             complement(1297..>1555)
                     /locus_tag="PLI69_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42341.1"
                     /translation="ESIFPFPMTSNKRHAEKVIYDILDSGNFGFYRSGKKRPKEKMAR
                     MWFSYVRGIKRTLKFGSISLQHFAIIPFTALFSRLKKGFKD"
BASE COUNT          382 a          405 c          338 g          430 t
ORIGIN      
        1 ccgggaatac attttccatg agcagctccc tttagcaatc cttttcttac ctcttatcac
       61 ttccacagcc tcagctatga catcctccgc tttgcaagtc tcaacgttgc gcaggttgcc
      121 atcgccccgg agtatcaggg tgcgtgtgtc gtgctttttc acccggtgaa gcacatagtt
      181 cccattttcc aactttacaa gcaaaatatt acctctctct attgagaagt tgtctacctt
      241 ccgcagcact atcttatctt ttccacctct tatcagggga aacatgctgc ttccttttcc
      301 tactatctct actcggttgc ccgatgctac catctccgcc acctcctgaa atagtgtact
      361 gttacttacc ttcgtagttt gtttagtgct cataatatta tgtcattgat taatacagaa
      421 tagaggcaga tagggcgact gcttccctat cgggcctatt gcgaagcctg tatagcggca
      481 ccttttcggc aacatccagc accgtagata tcacatactg tctatgggtc tgatcggact
      541 gcaataaggc agtcaattgg aatagttggg cgtatgcctc aaggcccaat ggtcctgtga
      601 gtatattctc gctatgctgc tccagctgga cgagggccgc cacttttgcg gaaacattgc
      661 gatagcaggg agtactaccg ctccagggtg cgccgaagac caccacctct ccatcatcca
      721 tgatgcgcac tataggctca tcgtcgttga gcagggagga gcccggtaca tacttgagcc
      781 acaacccact gtgcgtactc ttgcccgttc cgctctcccc gaggaatatc aacgctttgc
      841 cctccttctc aattaccgaa gcgtggaatt tcagagtccg gtaaggtgcc accgccatta
      901 aaaaagctat gcggagcagg gcaatgagga aaaagttctc ccctggcttt gtgagcgaca
      961 gattgctttg ataactactt ttatccgaag agacaaacat tatatattcc acaccatcgc
     1021 gctctattgc aacccacatg ttgccttcac ggtggtaaat attgaacatc actccatcga
     1081 ggcacacctc ctcgcttggc tcccactccg gcttctccaa cttcttgtta cctttgaaaa
     1141 taagcagggg tgctgattta tcgggagaag tagcctcacc taacgggggc gtaacccgga
     1201 agggagagaa atttggcaaa agtctggcag taacctccgg tgagggggtc tcaatggcca
     1261 tcaagtgcct cgcgatagta tagtataaaa gagtcgtcaa tccttaaatc ctttttttag
     1321 tcgagagaat agtgctgtga aaggaattat ggcaaagtgt tgcaaagaaa ttgaaccaaa
     1381 cttcagtgta cgctttatac cgcgaacata ggagaaccac attcttgcca ttttttcttt
     1441 cggacgtttt ttgccggacc tgtaaaagcc gaaatttccg ctatccagga tgtcatatat
     1501 gaccttctct gcatggcgtt tattggaggt cattggaaat ggaaaaatag actcc
//
LOCUS       DAOSIE010000321         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_70599_length_1541_cov_3.127860, whole genome shotgun sequence.
ACCESSION   DAOSIE010000321 DAOSIE010000000
VERSION     DAOSIE010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70599_length_1541_cov_3.127860"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="PLI69_07375"
     CDS             complement(<1..1506)
                     /locus_tag="PLI69_07375"
                     /inference="COORDINATES: protein motif:HMM:NF027574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4906 domain-containing protein"
                     /protein_id="HOG42342.1"
                     /translation="MEEICIDWPGLAGMTSVGFPMSHKGTFTVAQGNNTYTVDFTRLV
                     ARYDLIIDRSNMEGSFEVTGVKLRNSAAHAFPFSAKAATQTLDGDIATLSDIQRVNSG
                     SSVSFYMFENLQGILLPNNKDPWAKIPDNIPLAKDLCTYIEIEGEYIGGGVSTGLNYE
                     KMTFRIYLGADNTSSFDIMRNTMQTLTLFPSDNSISAQSWKVDPGIVADNRSIGWAPL
                     AINLLSMGKDTATLQLSPTSLPVILKGSNEFENGALHFSREGEKVIISSTSNNSTAIN
                     ATLTASTLDLKKSSTLRITVAPAVSVTQIIVEPSFLSLYYGKSEQLTVTAVWSDGSTS
                     DITAYCSWDNSIPGAALLSSSGMVTNIYSGSEDYAKGIITAEYMGEIGTVLVHCYRSG
                     YLHYSYLLNLDPTFAKVSLGSSMQITATLTTTTFIDGVEIDSSHEDVTNSAQWSSLHP
                     DKATVSAGEVTTLSIGTAAIMAEHSGETGFTILQIIPQSTPGITSITLSPSS"
BASE COUNT          388 a          328 c          374 g          451 t
ORIGIN      
        1 ggagctggga gagagtgtta tggatgttat accgggagta ctctgcggta ttatctgcaa
       61 tattgtgaat cccgtctctc cgctgtgctc tgccataatt gcagctgtcc ctatagatag
      121 agtggtgact tctcctgcag agactgttgc tttatcggga tgtaggctgc tccactgagc
      181 ggaatttgta acatcttcat gagatgagtc gatttctact ccgtcaataa aggtagtggt
      241 ggtcaatgtt gccgtaatct gcatggagga gccaagactc actttagcaa atgtggggtc
      301 gagatttagc agataactgt aatgcaggta gccgctacga taacaatgaa cgagtacggt
      361 accaatttcc cccatatatt ctgccgtaat tattccctta gcataatcct cacttcctga
      421 gtaaatgttt gttaccatac ccgaagagga gaggagagct gcaccgggta tgctgttatc
      481 ccacgaacag tacgccgtaa tgtcacttgt agagccatcg gaccatactg ctgtcaccgt
      541 aagctgttca ctctttccat agtataagga taagaaggat ggttctacaa taatctgcgt
      601 gacgctcact gccggcgcta cggttatccg tagtgtgctg ctctttttaa ggtcaagggt
      661 gcttgcggtc agagtagcat ttatagccgt gctattgttg gatgtgctgc taatgatcac
      721 tttttctccc tcacgggaaa agtgtagagc tccattttca aactcattac tgcccttgag
      781 gattaccggc agcgaagtag gggagagttg cagagtagct gtatcttttc ccatagataa
      841 aagatttatg gcgagtgggg cccagccgat gcttctgttg tccgccacaa ttcccggatc
      901 caccttccac gattgtgcgg agatggagtt gtcggaaggg aataatgtga gtgtttgcat
      961 agtatttcgc attatatcaa aagaagaggt attgtctgct cccagataga ttctgaaggt
     1021 catcttttca taattgagcc ctgttgaaac tcctcctcct atatattcac cctcaatttc
     1081 tatataagta cagagatcct ttgccaatgg aatgttgtcg ggaattttgg cccaggggtc
     1141 tttattgttt gggagcagaa tgccttggag gttttcgaac atataaaatg agacggaaga
     1201 gccgctattc acgcgttgaa tgtcggataa tgtggcaata tcaccatcaa gggtctgagt
     1261 agcagcttta gctgaaaatg gaaaggcatg ggctgccgag ttgcgaagtt taactccggt
     1321 tacctcgaaa ctgccttcca tattgctccg atctataatt aagtcatatc ttgcaacaag
     1381 gcgtgtaaaa tctactgtgt aagtgttatt accttgtgct actgtaaaag ttcctttgtg
     1441 ggacatagga aagccaactg aagtcatacc tgccagtccc ggccaatcta tgcaaatttc
     1501 ctccatttcg ctttccaaag cgggtggagt gaatcctatg a
//
LOCUS       DAOSIE010000322         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_71177_length_1533_cov_1.975643, whole genome shotgun sequence.
ACCESSION   DAOSIE010000322 DAOSIE010000000
VERSION     DAOSIE010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71177_length_1533_cov_1.975643"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLI69_07380"
     CDS             <1..595
                     /locus_tag="PLI69_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009293255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOG42343.1"
                     /translation="KVIKVKFGMTEKEDKRMINLIRAKTDTVICIDANQGWRDKHYAL
                     DMINWLAERNVNMIEQPMHKLMVDETAWLTERSPLPIIADEACQRLTDIPKLKGVYHG
                     INIKLMKCTGMREAREMVSLANALHMKIMVGCMTETSVAISAAAQIAPKVEWADLDGN
                     YLIANDCFDGMKLVDGRITLEDKPGLGLTKKPGIISL"
     gene            646..924
                     /locus_tag="PLI69_07385"
     CDS             646..924
                     /locus_tag="PLI69_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42344.1"
                     /translation="MNLRVIKKDIEFLIGDFVEDCLLFAMLHPDKDLESVEQLINKAS
                     DLADSLFYRVNHPDKSSKKAIKAHYKAVGHDLIAGLDGLCEELSALAK"
     gene            complement(1018..>1533)
                     /locus_tag="PLI69_07390"
     CDS             complement(1018..>1533)
                     /locus_tag="PLI69_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42345.1"
                     /translation="IQDWLPVNGKMIRFRFDWQPDYQGRVCYYVNATTDDPTIYFHKG
                     SGHDYPAPIPIGDEDLELLRRFVASMKVDNTYEDTTSPEGKGNWYLTINFDTGDILGI
                     NGRISNPRTEEEMRICDPIHDFFKPIIEKLEKQMLEINALQEDDAKYVPGGQQEQSSK
                     SATRKWWQFWK"
BASE COUNT          454 a          333 c          344 g          402 t
ORIGIN      
        1 aaaagtgatt aaagtaaagt tcggaatgac ggaaaaagag gacaagagaa tgataaacct
       61 catccgtgca aagacagata ccgtgatttg catcgatgcc aaccagggct ggagagacaa
      121 acactacgca ctcgacatga tcaactggct tgccgagaga aatgtcaata tgattgagca
      181 gcctatgcac aaactgatgg ttgatgagac tgcatggttg accgagcgca gtcctctacc
      241 catcattgcc gacgaagcct gtcagcgcct gaccgatatc cccaaattaa aaggtgtcta
      301 ccatggtatt aatataaagc tgatgaagtg tacgggaatg cgcgaagcac gagaaatggt
      361 ctcactggca aacgctctcc atatgaaaat catggttggt tgtatgaccg agacctccgt
      421 tgcaatttca gcggcagcac agatcgctcc gaaagtggag tgggcagatc tagacggcaa
      481 ctatctgatt gccaatgact gcttcgatgg aatgaagctg gtggacggac gtataaccct
      541 ggaggacaaa cctggtctgg gacttacgaa aaagcccggc atcatctctt tgtaaaaaat
      601 attattaatg ttacctttgc agccgaattt taaaggaata tagatatgaa tcttagagta
      661 attaagaagg acatagagtt tctcatcgga gattttgttg aagattgtct gctttttgcc
      721 atgctgcatc ctgataagga tcttgaaagc gtagaacaac tcataaataa ggccagcgac
      781 cttgcagata gcctctttta cagagtaaat catcctgaca agagtagtaa aaaggcaata
      841 aaagcccatt ataaggcagt aggccatgat ctgatagcag gtctggacgg cctatgtgag
      901 gaactttccg ctcttgcaaa ataggctttc tcatccaaaa gatttttaaa agcttaccaa
      961 acacaacggg gttgacatga tgtcagcccc gttgtgtttt aatttggtag aagtaaatta
     1021 tttccagaat tgccaccact tgcgagtggc agacttagaa ctttgctcct gttggcctcc
     1081 tgggacatac ttagcgtcat cctcctgaag agcatttatt tccagcatct gcttttccaa
     1141 tttttcaatt atcggtttga agaaatcgtg aataggatca cagatgcgca tttcctcttc
     1201 agtgcgagga ttgctgatcc tcccgtttat accgaggatg tctccagtat caaaattgat
     1261 ggtgagatac caattaccct ttccctccgg cgaagtagta tcctcataag tattgtctac
     1321 tttcatcgaa gccacaaagc gtctcagaag ctccaaatcc tcatctccta tgggtatggg
     1381 agcaggatag tcgtgaccgc tccctttatg gaaatagatt gtaggatcgt cagtcgttgc
     1441 attcacataa tagcagacac ggccttgata atcgggctgc caatcaaacc tgaaccttat
     1501 cattttacca ttaaccggga gccagtcctg aat
//
LOCUS       DAOSIE010000323         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_71749_length_1524_cov_3.460177, whole genome shotgun sequence.
ACCESSION   DAOSIE010000323 DAOSIE010000000
VERSION     DAOSIE010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71749_length_1524_cov_3.460177"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1524)
                     /locus_tag="PLI69_07395"
     CDS             complement(<1..>1524)
                     /locus_tag="PLI69_07395"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOG42346.1"
                     /translation="TEIQRDSLIKANSTPLIEAASKEGAGYSASVQPLYYGNQYFIWV
                     YQTFTDVRLVGAPPSSIGKFGGDTDNWMWPRHTGDFSLFRIYADKENNPAPYSEENVP
                     YKPKKYFKLSIKERKEGDFTFVYGYPGRTQEYIHSEAVRYISDISNPHKRNLRTKRLD
                     VQEKYMSQSQKVRIQYSSKNSGVSNAWKKWLGEMNGIIKLKTYEKKRAYEQEFHKWAA
                     DKPEYRDIIPQLDNLYEELEGYAFATDYYNESIRANEIIRFAENIITAFSRNRSKNYL
                     DQMIEAYFKDYFLPIDKESFHLLLAEYDKNVPKNLRPTYFSEQMNAFGSTDAWIEYIF
                     NTSALISEEGTRALVDKALTVEDFQEVLLADPAFTFSNEFRQHYMNEINRPFTEINRQ
                     ITLLYRTYMKGQMEFSPEKSFFPDANSTLRVSYGHIQGYSPADGIFFKPVSTIEGIME
                     KDNPDIFDYNIPQELRDIYARKDYGKWGANGTMPVCFIATNHTSGGNSGSPVIDADG"
BASE COUNT          406 a          333 c          339 g          446 t
ORIGIN      
        1 ttaccgtctg cgtcaataac cgggctgcct gagttgccac ctgaagtgtg gttagttgct
       61 ataaagcaga caggcatagt accatttgcc ccccatttac cataatcttt gcgtgcataa
      121 atatccctaa gttcctgggg tatattataa tcgaaaatgt ccggattgtc cttctccatt
      181 atgccctcga tggtggaaac gggcttaaag aagataccat cagcagggga ataaccctga
      241 atatggccat aagagactct tagagtggag tttgcgtccg ggaagaagga tttttcggga
      301 gaaaactcca tctgaccctt catgtaggta cgataaagca gagtaatctg cctattgatc
      361 tcggtaaacg gccggttgat ttcattcata tagtgttgcc tgaattcatt tgaaaatgta
      421 aaggcaggat cagccaggag tacctcctgg aaatcttcta cagtaagagc cttatccacc
      481 agagcacgtg tcccctcttc ggagatgagg gcagaagtgt taaaaatgta ctctatccag
      541 gcatcagtgc ttccgaaagc gttcatttgc tccgaaaaat aggtcggacg aagattcttg
      601 ggtacattct tgtcatattc tgcaaggaga agatgaaagc tctccttgtc gatgggaagg
      661 aaataatcct tgaaataggc ctctatcatc tggtcaagat aattcttact acgattgcga
      721 ctaaaagctg tgataatatt ttcagcgaag cgaataatct catttgcacg gatgctctcg
      781 ttgtaataat cggtagcgaa agcatagccc tcaagttcct catagagatt gtcgagctgt
      841 ggaataatat ccctatactc cggcttatcg gccgcccact tatggaattc ctgttcgtac
      901 gccctctttt tctcataagt cttgagtttg ataataccat tcatctcacc gagccatttt
      961 ttccacgcat tggagacacc tgaattcttg gaagagtact gtattctcac cttctggctc
     1021 tgcgacatat atttctcctg tacatcgaga cgttttgtgc ggagattacg tttatggggg
     1081 tttgagatgt cggagatgta tctaactgct tccgagtgta tatattcctg tgtccttccc
     1141 ggataaccat aaacgaaagt aaaatccccc tctttacgct ctttaatgga gagtttgaaa
     1201 tattttttag gcttgtaggg cacattctcc tcggagtaag gtgcgggatt attctctttg
     1261 tcagcatata ttctgaaaag agagaaatct ccggtatggc gaggccacat ccagttatcg
     1321 gtatcaccgc caaatttacc tatcgatgaa ggcggagcac ccactagacg tacgtcagta
     1381 aaagtctggt aaacccatat aaaatactgg ttaccataat atagtggctg cacggaagcg
     1441 ctatatccgg ctccttcctt tgaagcagct tcaatcagag gtgtgctgtt ggcctttatg
     1501 agtgaatcac gttgaatctc ggtc
//
LOCUS       DAOSIE010000324         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_71923_length_1522_cov_2.404226, whole genome shotgun sequence.
ACCESSION   DAOSIE010000324 DAOSIE010000000
VERSION     DAOSIE010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71923_length_1522_cov_2.404226"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLI69_07400"
     CDS             complement(<1..316)
                     /locus_tag="PLI69_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HOG42347.1"
                     /translation="MKKIFITAIAIMSISACSQKNVFLSEWDTPYGIPPYDKIKISDY
                     LPAIKEGIKQHDAEIEAITSCVETPTFENTIAPLELSGELLQKVTGVLFNVSETDRSD
                     ALD"
     gene            complement(320..1417)
                     /locus_tag="PLI69_07405"
     CDS             complement(320..1417)
                     /locus_tag="PLI69_07405"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOG42348.1"
                     /translation="MKKILLAILATAAFFTACEEWQPVGTFKYSEPEELPLITDADMA
                     GYGQRITIKDLCGRYVNGTPLKLESGWIKGQVISNDASGNIYRSLYIQDETGGIEIKT
                     GRTNSSNEYKMGQWLYVKLSGLTLGMYGFKTGNYGGQGMIQLGLTDQSGAYETAYIDI
                     PLIVDSHILKGEMGEPVAPVKLTAAQLPGKNDTQATNKYIGTLVELEGLKYGNEIFTL
                     IYLSYSQDTKAATNRIFLTEQTWGITTWGLSKEKMGKLLEAGTWDEAYVGSGNETHGK
                     VGDHRTVEGINGATYGDIERNAYSVSHYFKMGATDIQVRSSGYARFADVEIDPGILSG
                     ANAVNFTGILTMYQGSIQFTLLDLDGVVKVQ"
BASE COUNT          365 a          349 c          345 g          463 t
ORIGIN      
        1 tgtcgagtgc gtcgctgcgg tcggtttcag acacattgaa aagcactccc gtcacttttt
       61 ggagcaattc tccgctgagt tcgagcggag caatggtgtt ttcgaatgta ggggtctcca
      121 cgcaagaggt gattgcctct atttcggcat catgctgctt gattccttct ttgattgcgg
      181 gaagataatc cgaaattttt attttgtcgt atggagggat gccgtaaggg gtgtcccatt
      241 cagagagaaa aacgttcttt tgcgaacatg cagagatgct cataattgcg attgctgtga
      301 taaaaatctt tttcatttat tattgtactt taactacgcc atctaaatct aaaagagtga
      361 attgtatgct tccttgatac atggtcagaa tacctgtaaa attaactgcg tttgcaccgc
      421 tcaaaattcc gggatctatt tccacatcgg cgaatctggc ataaccgctg ctgcgtacct
      481 gaatatctgt agcgcccatc ttgaaatagt gactgacact ataagcattg cgctcgatat
      541 caccgtaagt ggcaccgttt ataccttcga ctgttctatg atctccgacc ttgccgtgcg
      601 tttcgtttcc gctgccgaca taagcctcat cccaagtgcc tgcctcgagt aatttaccca
      661 tcttttcctt gctaaggccc caagtagtaa tgccccaggt ttgttcggta aggaaaatgc
      721 ggttggtggc agccttggtg tcctggctgt aagacagata gatgagagtg aatatctcat
      781 tgccgtattt cagaccctca agttcaacca gagtgcctat atacttattg gtagcctggg
      841 tgtcgttttt gcccgggagt tgtgctgcgg taagctttac aggcgctaca ggctctccca
      901 tttctccctt cagaatatga ctgtcaacta tcaaaggaat gtcgatatag gctgtttcgt
      961 aggctccgct ctggtctgtg agaccaagtt gaatcattcc ctgaccgccg taattgccgg
     1021 ttttgaaacc atacataccg agagtaaggc cgctcagttt cacatatagc cactggccca
     1081 tcttgtattc attggaggaa ttggtgcggc ctgtcttgat ttcgattccg cctgtctcgt
     1141 cctgaatata cagagaacgg taaatgttgc ctgaggcatc gttactgata acctgaccct
     1201 ttatccaacc ggattcgagt ttaaggggtg ttccattgac atatctgccg caaaggtctt
     1261 ttatcgtgat tcgctgtccg tagccagcca tatcggcatc ggtgatcaat ggcaattcct
     1321 ccggctcgga atatttgaaa gtgcctacag gctgccattc ttcgcaggcc gtgaaaaacg
     1381 ccgctgttgc gagtattgct aataatatct ttttcataag gccttaaaat ctaaaataaa
     1441 catttaacat ataattgaaa cctgcataat agaaatacct tggatcgaag cggtaatagc
     1501 tggtcttgct tgtagtgttt tc
//
LOCUS       DAOSIE010000325         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_73113_length_1505_cov_3.539310, whole genome shotgun sequence.
ACCESSION   DAOSIE010000325 DAOSIE010000000
VERSION     DAOSIE010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73113_length_1505_cov_3.539310"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1505)
                     /locus_tag="PLI69_07410"
     CDS             complement(<1..>1505)
                     /locus_tag="PLI69_07410"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOG42349.1"
                     /translation="LRYTFPKVATDSLITLLNIPSWIVPNLEYDEDVTALLHRDAVNY
                     ISPTKGIIYSGQLIVSKGEIVSAEIEQILDSYKAEYNRSFGYSSSRGWLIVSHILTVL
                     ILVALLFLAIFFTAKEVLRRTSHLLFILLMVFFSFAATALVHRLGANLIYLVPFAALV
                     LIAASFFRNELIFPVYVISLLPLLLIAENGIELFGINLVAGSIGLLTYAHFNRGWKLF
                     LNAVFIFAGMVALLLSFRLLTDDAAMIWKSYTVTFIAINAVLCVVLNPFVLLFERIFS
                     FTSYSRLFDLTDSSNPLLQELSHRAPGTFQHSLMVANMAENAAREIGANAMLARAGAM
                     YHDVGKIENPACFIENVNPGSGQDNYHKGLTPEESAADIIRHVDDGLALARKFHLPDV
                     IKDFIRSHHGQSMTLYFYNVYCNNGGDPKNTAPFTYRGMLPTTKEQVVVMMADAVEAA
                     SRSLKKYTDESISELVEEILGSRLSDQQLVRADISLKEINIVKECFKRQIK"
BASE COUNT          451 a          337 c          355 g          362 t
ORIGIN      
        1 tgcttgatct gccgtttaaa gcactctttg actatattta tttccttgag tgagatgtcc
       61 gcgcggacaa gctgttggtc tgagagacgt gaaccgagaa tctcttcaac cagttcggag
      121 atactttcat cggtatattt cttgagtgaa cgcgaggcgg cctccacagc atccgccatc
      181 atcaccacta cctgttcctt ggtagtagga agcattcctc tatatgtaaa aggtgctgtg
      241 ttcttaggat cacctccgtt attgcaataa acattataaa aatagagagt catcgattgt
      301 ccgtgatgcg agcgtatgaa gtcttttatc acatccggaa gatggaattt gcgggcaagc
      361 gcaagtccgt catcaacgtg acggattata tctgcagcgc tctcttccgg tgtaagtcct
      421 ttatggtaat tatcctgtcc gctaccggga ttgacatttt caatgaagca agcgggattt
      481 tcaatttttc ccacatcatg gtacattgca cctgctcttg ccaacatggc attggcacca
      541 atttcccgcg cagcattttc ggccatattt gccaccataa gagagtgctg gaaagtgcct
      601 ggtgcgcggt gcgatagttc ctgaagcaga ggattgctgc tatcggtaag gtcaaagaga
      661 cgggagtatg aggtaaatga aaatatcctt tcaaagagga ggacaaaggg attgagaaca
      721 acgcataaca ctgcattgat agcaatgaat gtcacagtat aactcttcca gatcatcgct
      781 gcatcatccg tcaatagacg gaatgagagc aacagagcca ccattcccgc aaagatgaag
      841 accgcattga gaaacaactt ccatcctctg ttgaaatggg cataagtaag cagtccgata
      901 ctgccggcca ccagatttat gccaaacagt tctataccat tctctgcgat tagcaaaaga
      961 ggcaaaagcg atattacata gacgggaaag ataagttcat tcctaaaaaa ggaggcagca
     1021 atgaggacca gcgccgcaaa aggcaccaga taaatgagat tggctccaag acggtggacc
     1081 aaagccgtag ctgcaaagga gaaaaatacc atcaacagga taaacaacag gtgactggtg
     1141 cgtctcagca cctcctttgc agtgaagaaa atagccaaaa agagaagtgc aaccaatatg
     1201 agcacagtca atatatgact gacgatgagc catccgcgcg aagagctgta accaaaactt
     1261 ctgttgtact cagccttgta ggaatcgaga atctgttcga tctcagccga gactatctcc
     1321 cccttggaga caatcagctg accggagtaa ataataccct tggtgggcga aatgtaattg
     1381 actgcatcgc ggtgtaaaag cgcagtaaca tcctcatcgt actcgaggtt tggcacaatc
     1441 caggagggaa tattaagtaa tgttataagc gaatcggtgg ccaccttggg aaaagtatag
     1501 cgcag
//
LOCUS       DAOSIE010000326         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_73361_length_1502_cov_3.900484, whole genome shotgun sequence.
ACCESSION   DAOSIE010000326 DAOSIE010000000
VERSION     DAOSIE010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73361_length_1502_cov_3.900484"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLI69_07415"
     CDS             <1..329
                     /locus_tag="PLI69_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42350.1"
                     /translation="FRREMRGVEGAYTLGRAVCGRIVYEAGVYLVVINRIPRKVVDVG
                     KAACGDVADFGFEPVDSSFYVGRVAADGLRIWIINILGINASRERHSRRPAVCADAQD
                     YGYAKE"
     gene            complement(340..723)
                     /locus_tag="PLI69_07420"
     CDS             complement(340..723)
                     /locus_tag="PLI69_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42351.1"
                     /translation="MRKYIVPFLTIVLVALSINVYALQKQSAAAPGEVLDSDGDETTA
                     VQMTGLFAVEATSTGVGTIQIKRSEDNSSNWIVVKTLTNASAGDQRKYLYEYFGDEDS
                     SKGAWYKAVLSGNPTSGSFRVRLVK"
     gene            complement(855..>1502)
                     /locus_tag="PLI69_07425"
     CDS             complement(855..>1502)
                     /locus_tag="PLI69_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42352.1"
                     /translation="LFTQDAQKNLAGIMKRANSINTRIFTNKAEAFEFLDTLTGVSNE
                     KVIDYIFKPNNTRYITYAKRLLSPERNIELQEQALRKVLKATPEGNILPVQSLNKFKQ
                     YNEPLRKLLPDDLYRDVESFLKMAGHSKKIEAMALNASQTGQIFLGHQLLQMLLGAPV
                     KLAGATGVPWAIAKIYVNPKARFFMKRALVAPDKEATGNFIRALEIAGADMMGED"
BASE COUNT          333 a          386 c          367 g          416 t
ORIGIN      
        1 ctttccgccg agaaatgaga ggcgttgaag gtgcatacac ccttggtcga gccgtctgcg
       61 gccgcattgt ttatgaggct ggagtctatc ttgttgtcat caaccgtatc ccccggaagg
      121 tcgtcgatgt cggcaaggct gcctgcggag atgttgccga tttcggtttt gagcccgtcg
      181 atagcagttt ttacgttggt agggttgccg ctgatggact tcgtatttgg ataatcaaca
      241 tcctcggcat caatgcttcc cgtgaacggc actcccgccg tccagccgta tgtgcagacg
      301 cacaggatta tggttatgca aaggagtagt ttcttcatgt tatttcacca gcctgacgcg
      361 gaatgagccg gaagtggggt tgccggagag gacggctttg taccatgcgc ccttggacga
      421 atcctcgtca ccgaaatact catagaggta cttgcgctga tccccggcag atgcattagt
      481 gagcgtcttg accactatcc aattcgacga attgtcttct gatcgtttga tctggatggt
      541 gcccactccg gtgctcgtcg cctcgacagc gaaaagtcct gtcatctgca cggctgtcgt
      601 ttcatccccg tcagagtcca aaacctcgcc tggcgctgcg gcggactgct tctggagagc
      661 atagacattg atggacaaag ccaccaggac aatggtaagg aatggtacta tgtactttct
      721 catctggctc actcctttgt gatgtgctac ctatactgtt gcttgaggag tgagttgatg
      781 aatcggatga aaaattccaa agcgatttac gccacgagcg ggtatgcaaa catcattgaa
      841 gcgtagatag ccttctagtc ttcccccatc atatcggcac cggcaatctc cagcgcacgg
      901 ataaagtttc cggtcgcctc cttatccggt gccaccagcg ctctcttcat gaaaaatctg
      961 gccttcggat tcacatatat cttcgcaatc gcccaaggta cccccgtcgc accggcgagt
     1021 ttaacaggcg cgccgagcag catctgcaac aactgatgcc caaggaaaat ctgcccggtt
     1081 tgcgacgcat tgagggccat tgcctctatc ttcttgctat ggcctgccat cttcaggaat
     1141 gactctacat ccctgtataa atcatcgggc aagagcttcc tcagcggctc gttatactgt
     1201 ttgaatttat tgagggactg aactggcaga atgttaccct ctggtgttgc cttcaatacc
     1261 tttctcaatg cctgctcttg tagttcaata ttgcgctctg gagagagaag cctcttggca
     1321 tatgtgatgt acctggtgtt attcggctta aatatgtagt ctattacctt ttcatttgaa
     1381 acacctgtca atgtgtcaag aaattcaaac gcttcagcct tattcgtgaa aatcctagtg
     1441 ttgattgaat ttgccctctt catgataccg gccaaatttt tctgagcatc ttgcgtgaaa
     1501 ag
//
LOCUS       DAOSIE010000327         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_74053_length_1493_cov_2.863004, whole genome shotgun sequence.
ACCESSION   DAOSIE010000327 DAOSIE010000000
VERSION     DAOSIE010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74053_length_1493_cov_2.863004"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLI69_07430"
     CDS             <1..412
                     /locus_tag="PLI69_07430"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOG42353.1"
                     /translation="EEINRLEQEYLTLFFGITETSTQKMSFDVVPQAGNDKHIYVAFR
                     LSETQGLLPASNMSGRPIILELTLDEGAKGTTIQQAAAAAAGRSRSQMIYYRVPATAT
                     ARILDGQQVLLQTRVPVYQLGTVRSFPIETLISK"
     gene            449..>1493
                     /gene="pepD"
                     /locus_tag="PLI69_07435"
     CDS             449..>1493
                     /gene="pepD"
                     /locus_tag="PLI69_07435"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HOG42354.1"
                     /translation="MTLSHLKPQRVWKHFYSLTQIPRPSKHEGKAVQFLEKFGKELGL
                     ETIKDEIGNVIIRKPATPGMENRKGIILQAHIDMVPQKNNDKVHDFKKDPIQPRIVNK
                     EDGEWLYATGTTLGADNGLGCALAMAVMEAKDLVHGPVEALFTVDEETGMTGARGLKP
                     GILKGDILINLDSETEGELYVGCAGGLDATAVFKYREKPLPKGHEVLAIHVKGLRGGH
                     SGMEIHEGRGNANKVMMRVLLPALRDLKAKLVSIEGGNLRNAIPREAVAVVALPPAKV
                     KGLQAIVDSVLTKVKNEYANIDPNIEIFLEKTKGKNMMCGQVALKMAKAVYACPNGVD
                     RMSLEMADLVETSN"
BASE COUNT          430 a          361 c          377 g          325 t
ORIGIN      
        1 agaagagata aaccgtctgg aacaggagta ccttactcta tttttcggta tcactgagac
       61 ctccacccag aaaatgagtt tcgatgtggt gcctcaggca ggcaatgaca agcatatata
      121 cgtggctttc cgtctttcgg agactcaggg actccttccc gcctccaaca tgtccggacg
      181 accgatcatc ctcgagctca ccctcgacga aggggcaaaa ggcactacta ttcaacaggc
      241 cgctgcagct gctgccggaa ggagcagaag ccagatgatc tattacaggg tacctgctac
      301 ggctactgcg cgtattctgg atggacaaca ggtgttacta cagaccagag tgcctgttta
      361 ccagctgggg acggtacgct ctttccccat agaaacactc attagcaaat aatccattta
      421 ttaatcaaat aactaagtat aaaacagtat gacgctttct catctaaaac ctcagagagt
      481 gtggaaacat ttctactccc tcacacagat tccccgtcct tcgaagcatg aaggcaaagc
      541 agttcaattc cttgagaaat tcggtaagga gcttggtctg gagaccatca aggatgaaat
      601 tggcaatgta ataatccgca agcctgctac tcccggtatg gaaaacagga agggtatcat
      661 tttgcaggca catatagata tggtacccca gaaaaacaac gacaaggtac acgacttcaa
      721 aaaggatccc atccagcccc gtattgtaaa taaagaggat ggtgagtggc tttatgccac
      781 cggcaccact ctcggagcag acaacggact cggctgcgct ttggcaatgg cggtaatgga
      841 ggcaaaagat cttgtacatg gccctgtgga ggcacttttc acagtggatg aggagaccgg
      901 tatgaccggt gcgagaggac tcaaaccggg tatattgaag ggcgacatcc ttataaacct
      961 cgactctgag accgaaggcg aactttatgt gggttgcgcc ggcggactcg atgccactgc
     1021 ggtattcaaa tatagagaga aacctcttcc caagggacac gaagtattgg caatccatgt
     1081 caaaggactt cgcggaggcc actccggtat ggagatacac gaaggcaggg gcaatgcaaa
     1141 taaggtgatg atgcgagtcc tgctcccggc ccttagggat ctgaaggcca agctcgtctc
     1201 tatagagggc ggcaacctgc gcaacgccat cccacgcgag gcggtagcag tggtagctct
     1261 acctccggca aaagttaaag gacttcaggc aatagttgat tcggttctaa ccaaagttaa
     1321 gaatgaatat gccaatatag accccaatat tgagattttc ctcgaaaaga ctaagggaaa
     1381 gaatatgatg tgcggtcaag tggctctcaa gatggccaag gctgtatatg cctgccccaa
     1441 cggagttgac agaatgagcc tcgagatggc tgaccttgtg gaaacttcca aca
//
LOCUS       DAOSIE010000328         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_74116_length_1492_cov_3.463466, whole genome shotgun sequence.
ACCESSION   DAOSIE010000328 DAOSIE010000000
VERSION     DAOSIE010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74116_length_1492_cov_3.463466"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PLI69_07440"
     CDS             <1..1272
                     /locus_tag="PLI69_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOG42355.1"
                     /translation="LSVILFSGGLDTRFTEIRPVMWQGISLSTIGVMLMALITGVFIW
                     LLTRNGAFSFTGSLALCLLLASTMSSTDSASVFNLLRTQKVGLKHNLKPLLEFESGSN
                     DPMAYMLTVALIQFCKFGELSTWNIVFHFILQFVLGLLIGFLIGKGAIWVINRINLNN
                     VALYPVIMLSIVFFTFSVTELAKGNGYLAVYIAGIIMGNSPFVRKRECMKFMDGITWL
                     CQAIVFLTLGLLVEPRSLIEVALPATLIGLFMIFFARPLTVMITLLPFKQPTFKPKVF
                     VSWVGLRGAVPIIFATYPVVAEVPGAYVIFNIVFFITILSLLIQGTTITWVAKLLGVA
                     TDLPKEGNLFGVELPEEIDSKLWDVTVTEELLANGNQLKDMNLPQGVLVIMIKRGDAY
                     LVPNGTLEILSGDHLLLISQSDAEEEHALQQ"
BASE COUNT          384 a          314 c          326 g          468 t
ORIGIN      
        1 ttaagtgtca ttcttttttc gggtggtctg gatacgcggt ttacagagat aaggcctgtc
       61 atgtggcaag gtatttctct ttccacaatc ggggtaatgt tgatggcatt gattaccggg
      121 gtgttcattt ggctgcttac gcgcaatggc gcattctcct tcaccggatc attggcactc
      181 tgtctgttgc ttgcctccac catgtcctct accgactccg cttctgtatt taaccttctg
      241 agaacccaga aagtagggct caaacacaat ttaaagcctt tactcgagtt tgaaagtggt
      301 agcaatgacc ctatggccta tatgctgacc gtagcgctca tacaattctg caagtttggt
      361 gaattaagca catggaacat cgtattccac ttcatactac agtttgtttt aggtctgctt
      421 atcggttttc ttataggcaa gggtgccatc tgggttataa ataggattaa cctcaacaat
      481 gtcgccctat accccgtaat aatgctcagt atagtcttct ttaccttctc cgttacagaa
      541 ttagctaaag gtaacggtta tcttgcagtc tatattgccg gcattataat gggaaactct
      601 ccatttgtaa gaaagaggga atgtatgaag tttatggatg gtatcacctg gctctgccag
      661 gctatagtat ttctcaccct cgggctgctg gttgagcctc gctcattgat tgaggtggct
      721 cttcccgcca ctttgattgg cttatttatg atattctttg cccgcccctt gactgtgatg
      781 ataacgcttc taccctttaa gcagcccact ttcaaaccca aggttttcgt ctcttgggta
      841 gggctgaggg gagcagtacc tatcattttc gcaacttacc ccgtagtagc cgaagtacca
      901 ggagcatatg tgatctttaa tatagtattt ttcataacca ttctctcatt gttgattcag
      961 ggtacgacta ttacatgggt ggctaaatta cttggcgtgg caaccgatct tccaaaagaa
     1021 ggcaatcttt tcggagtgga acttccggaa gagattgact caaaactctg ggatgttacc
     1081 gttactgagg aactgcttgc caatggtaac caacttaagg atatgaatct ccctcagggg
     1141 gtactggtga taatgattaa gagaggtgac gcatatctgg tgcctaacgg cactttggaa
     1201 attctttccg gcgaccatct tctgcttata tcacagagtg atgccgaaga ggaacacgca
     1261 ttacagcagt agctgttggc tgttggctgt tggctgttga caattaagaa ttaagaatta
     1321 agaattttta tgatagcaat aactttaaaa aaaactatac tatgaaaaaa attacaattt
     1381 tatttgttgc tttcatttcg attatggcag catcctgcaa gtcaaatagt gataatgatg
     1441 gcaatccggt gatcactgta aacaatgccg aagaatttat caaagctctc gg
//
LOCUS       DAOSIE010000329         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_74929_length_1481_cov_3.382889, whole genome shotgun sequence.
ACCESSION   DAOSIE010000329 DAOSIE010000000
VERSION     DAOSIE010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74929_length_1481_cov_3.382889"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..989
                     /locus_tag="PLI69_07445"
     CDS             69..989
                     /locus_tag="PLI69_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133901.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOG42356.1"
                     /translation="MKKRYNSFVGYYKSKYGSRLQKIVVDAGFTCPNRDGSISVGGCT
                     YCDNNAFHPSYSTPDKSITQQMEEGMEFHRRRYRTASSYIAYFQPYSNTYTPLERLKE
                     LYAEALSHPNVVGIAVGTRPDCIDEEKLDYLAELSQKYIVMVEYGIESCYDATLERIN
                     RGHTFEQAARAVRMSAERGLDVCAHFILGLPGESREMMLDSASVINSLPLTSLKFHQL
                     QIIKGTRMEYEFEAQPEEFVTFTLDEYLDFFVDMLERLRPDIRIERFAGEVPPRFVKS
                     TPWGLIRNVELLRLLEKRLEERDSWQGKLL"
     gene            1038..>1481
                     /locus_tag="PLI69_07450"
     CDS             1038..>1481
                     /locus_tag="PLI69_07450"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOG42357.1"
                     /translation="MEHYSDKIIMEGLAYDDVLLIPAESGVLPREVDITTQFTREIPL
                     HIPIVSAAMDTVTDANMAIAIAREGGIGVIHKNMPIEQQMEQVRKVKRAENAMIYDPV
                     TIKYDASVGDALQLMAENRIGGIPVVDDQNYLIGIVTNRDLRFQEN"
BASE COUNT          419 a          309 c          352 g          401 t
ORIGIN      
        1 ttaaaattat tggtttaaac ttcaaaaata ctctttttct tatctttgtg caagaatagg
       61 cagcaaaaat gaaaaaaaga tataactcct ttgtcggata ctacaagagt aaatatggca
      121 gcagactgca aaaaatagtg gtagatgccg gttttacctg tcccaatcgt gacggatcaa
      181 tttctgtcgg gggatgtacc tattgtgaca ataatgcttt ccacccttca tacagtactc
      241 ctgacaagag catcacgcag cagatggagg aggggatgga gtttcaccgt cgcagatatc
      301 gtactgcaag cagttatatt gcctacttcc agccctattc caatacctat actccgcttg
      361 agcgcttaaa agaactctac gcggaggctc tttcacatcc caatgtggtg ggcatagcgg
      421 tgggaacccg tcccgactgt attgatgaag agaagcttga ttatcttgca gaactctcgc
      481 agaaatacat agtgatggta gagtacggta tcgaatcgtg ctacgatgcc actttggagc
      541 gaatcaatcg tggtcataca tttgaacagg ctgcccgtgc cgtcaggatg agcgcagagc
      601 gcggactcga tgtttgtgcg cattttattt tagggcttcc cggagagagc agggagatga
      661 tgcttgatag tgcttcagta attaattcgt tgccactaac ctcgctcaaa tttcaccaac
      721 tgcagataat caaaggtacc cgtatggagt atgagttcga ggctcaaccg gaagagttcg
      781 tcacttttac attggacgaa tatctcgatt tttttgtgga tatgctcgaa cgtctacggc
      841 ccgatatccg tatcgaaaga tttgccgggg aggtgcctcc aaggtttgtc aaatccactc
      901 catggggtct tattcgcaat gtggaattgc ttagattact tgaaaaaagg ctcgaagagc
      961 gtgatagttg gcagggcaaa ttgctctgaa acgaactttt gttatatttg tagtttataa
     1021 ttgataatta ttgcgatatg gaacattatt ccgacaagat cataatggaa ggattggctt
     1081 acgatgacgt attgttgatt ccggccgagt caggtgtttt accgagggaa gttgatatta
     1141 caactcaatt taccagagag atacctctcc acattcctat agtatccgca gccatggata
     1201 cggtcactga cgcaaatatg gcgattgcca tagcacgcga aggcggtatt ggcgttatcc
     1261 acaaaaatat gcccatcgaa cagcagatgg agcaggtacg taaggtcaaa cgtgccgaga
     1321 acgccatgat atacgatcct gtcacaatta aatatgatgc ttcggtaggc gatgcactcc
     1381 aactgatggc agaaaaccga ataggcggta tccccgttgt ggatgatcag aattatctga
     1441 taggtatcgt caccaacagg gatctccgct ttcaggaaaa c
//
LOCUS       DAOSIE010000330         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_76295_length_1464_cov_2.303052, whole genome shotgun sequence.
ACCESSION   DAOSIE010000330 DAOSIE010000000
VERSION     DAOSIE010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76295_length_1464_cov_2.303052"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1464
                     /locus_tag="PLI69_07455"
     CDS             <1..>1464
                     /locus_tag="PLI69_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42358.1"
                     /translation="VYNGNGSITELEGFKIGHDCYMPEDDEVTWRDIVSGEMKSSRLG
                     KMDKRYLHDLSLKYSSITANDWDLRASYHLCLMTPSQYTRIVLSSIHKDGEKFVQNRL
                     VTVEDQKTFDNEFLLEVRKRFSRHNLRLGLDIIYAKQYDAGSTFNFAHTVEANPQRLK
                     HNGAYTWNYNNSGLYLDAQRMSVVVYGMDQWQLGHNLRLSTGLRVRYLSNSPICAPFD
                     RETGINNRQTGDFYLGSPNCELKEIPINGFDYSVSEALSWRLTGRLFANAEGFYSLTN
                     KSTLYFKGANIPSLKAIGNAYARCGLSYDNEWMDVACLFSYITSWNNAKIMDVSDSST
                     GEIIPWTAQYGIRTFGVTLDGNIHFGGFKLHMLGTWQDPRYKNYRNEFNFSGGTKIID
                     YSGKFVTGISQGMIEFDPSYSWSRYKVWSSIRYYSKQYASRNNLAYFDGHFETFAGFS
                     ANLKKYGELALNIVNVFGDKGANGSLDIVDTIEDPDI"
BASE COUNT          428 a          304 c          329 g          403 t
ORIGIN      
        1 tcgtttacaa cggtaacggc agcatcactg aacttgaagg ctttaaaatc ggccatgatt
       61 gctatatgcc cgaggatgat gaagttacct ggcgcgacat agtatcggga gaaatgaagt
      121 cttcaaggct tggcaagatg gacaagcgct acttgcacga tttgtcgctc aagtattcat
      181 ctattacagc caatgactgg gatttgaggg cctcctatca cctttgtctt atgactccaa
      241 gccagtatac gcgcatagta ttgtcatcaa ttcacaaaga tggtgagaag ttcgtacaaa
      301 acaggcttgt gacagtagag gaccaaaaga cttttgacaa tgaatttttg ttagaggtca
      361 gaaagagatt ctcccgtcat aatctaagac ttggtttgga tatcatatat gccaagcagt
      421 atgatgccgg ctccactttt aattttgctc atacggttga ggcaaatcct caaaggctta
      481 aacacaatgg agcatatacc tggaattaca acaacagcgg gctttatctt gatgctcagc
      541 gcatgtcagt agtcgtgtac ggaatggacc aatggcaact tggccacaat ctcagactta
      601 gcaccggtct aagagtcagg tatctgtcaa acagtccaat ctgcgcacca ttcgaccgag
      661 agaccggtat taacaatagg caaacaggtg atttctatct cggtagtccg aactgtgaac
      721 ttaaggaaat accgattaac ggttttgatt attctgtgtc tgaggctttg tcctggcgtt
      781 tgacaggcag gctgtttgcc aatgcggaag gcttttacag tctcactaac aaatccaccc
      841 tatatttcaa aggtgcaaat attccatcac tcaaggccat aggcaatgct tatgcccgat
      901 gcggtttgtc ttacgataac gaatggatgg atgtggcctg ccttttttct tatatcacca
      961 gttggaataa tgccaagatt atggatgtat ctgacagttc taccggagaa atcatccctt
     1021 ggacagccca gtacggcatt cgtactttcg gcgtgactct agacgggaac attcatttcg
     1081 gcggattcaa acttcatatg ctgggcacat ggcaagatcc acgatacaaa aactatcgca
     1141 atgagttcaa cttttcaggt ggaacaaaaa taatagatta ttcaggcaaa ttcgttaccg
     1201 gaatatctca aggcatgatc gaatttgacc cctcctattc atggagcaga tacaaggttt
     1261 ggtcaagtat ccgatattat agcaaacaat atgcaagcag aaacaatttg gcatattttg
     1321 acggtcattt cgagaccttt gcgggtttta gcgcaaatct gaaaaagtat ggagaattgg
     1381 ctcttaatat agttaatgtg tttggggata aaggagccaa cggcagtctg gatattgtcg
     1441 atacaataga ggaccctgac atcc
//
LOCUS       DAOSIE010000331         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_77342_length_1450_cov_4.030824, whole genome shotgun sequence.
ACCESSION   DAOSIE010000331 DAOSIE010000000
VERSION     DAOSIE010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77342_length_1450_cov_4.030824"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..380)
                     /locus_tag="PLI69_07460"
     CDS             complement(147..380)
                     /locus_tag="PLI69_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOG42359.1"
                     /translation="MAEKNASPQSHAHEITRIPVGTEVRAAYLFSEKDVRIDGTFTGD
                     IITSEKLIIGETGYLTAISVVKMQISMVVLTVT"
     gene            complement(406..>1450)
                     /locus_tag="PLI69_07465"
     CDS             complement(406..>1450)
                     /locus_tag="PLI69_07465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG42360.1"
                     /translation="PGKQLQVTSDDGSIAIYSADHIIIATGARAASLPFAPIDGNKII
                     SYRQALVPSKLPKSLVVIGSGAIGSEFAFFYRALGVEVTLIEYLDRVLPIEDDEVSAQ
                     VSRSFRKMGMKVMVSSQVKSVDTTGELSRITVLTKKGEEIVEAELVLSAVGVVPNTEN
                     LGLEAAGVETVKGKITVDQYYRSSIEGVYAIGDVISTPALAHVASAEAVCCVEAIAGL
                     NPRPIDYSIIPACTYISPEVASVGMTERAAKEKGIAYKVGKFPFTASGKATAAGERDG
                     FAKMVVDEQTDRVLGVHIVGANASEMIGGMVLACKLGATASSVMKTIFPHPTMSEAIM
                     EAAAATHSEAIHI"
BASE COUNT          368 a          357 c          321 g          404 t
ORIGIN      
        1 ctcttcctcc attttttggt actcctcctg agtgataatt ttacaggtac cgttgaaacg
       61 tgcacctctc tcaacactta acttgtgagt ctgaaggagt cctttgaata tgcaggtgct
      121 ctgtaacatc agggtgtcac ctattatcac gtcaccgtca atacgaccat agatatctgc
      181 atttttacaa cagatattgc cgtaagatat cctgtctcac caataatcaa tttttccgaa
      241 gtgattatat caccggtaaa tgtaccgtca atccttacat ccttctcgga aaagaggtaa
      301 gcagcacgga cttctgtgcc cacgggtatt cgggttattt cgtgggcatg tgattgaggg
      361 gacgcatttt tttctgccat aatccgaaag ttaaaataat taggtttata tatgtattgc
      421 ctcggagtgc gttgccgcag ctgcctccat aatggcttca ctcatagtgg ggtgaggaaa
      481 aatggtcttc attacggagg atgcagttgc accgagtttg caggcaagca ccattcctcc
      541 aatcatctca gatgcattgg ctcctacaat atggactccg agtacgcggt cggtttgctc
      601 gtccacaacc attttggcga atccatccct ttctccggct gcggtagcct tacctgaagc
      661 agtgaaggga aatttaccta ctttatatgc tatgcccttc tctttggcag ccctttcggt
      721 catacctact gaggcaacct caggtgaaat ataggtgcag gcaggaatta tcgaatagtc
      781 gataggtcgg gggttgagtc ccgctatggc ttctacacag cagacagctt cagctgaggc
      841 aacgtgagcc agtgccggtg tggaaattac atccccgata gcataaactc cttcaattga
      901 gctcctataa tattgatcca cggtgatttt accctttacg gtctctacgc ccgcagcttc
      961 gagccccaaa ttttcggtat ttggaaccac accaacggca gagagtacca attcagcctc
     1021 tacaatctct tctccttttt tcgtaaggac tgtaatacga gacagctctc cggtagtgtc
     1081 aacgctcttg acctgggagg agaccatgac cttcatcccc atttttcgga aagaacggct
     1141 gacctgagcg cttacctcat catcctctat ggggagaact ctgtcaaggt attcgataag
     1201 ggtaacttcc actccaaggg ctctataaaa gaatgcaaac tcactaccaa ttgcgccgga
     1261 ccctattacc accaaactct tgggaagttt ggatggtaca agagcctgtc ggtaggaaat
     1321 aatcttatta ccgtcgatgg gagcaaatgg cagggaagca gctcttgcac cggtagcaat
     1381 tatgatatgg tctgcactgt aaatagcaat actaccatca tctgaagtaa cctggagctg
     1441 tttaccgggg
//
LOCUS       DAOSIE010000332         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_78009_length_1442_cov_3.167267, whole genome shotgun sequence.
ACCESSION   DAOSIE010000332 DAOSIE010000000
VERSION     DAOSIE010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78009_length_1442_cov_3.167267"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="PLI69_07470"
     CDS             <1..1401
                     /locus_tag="PLI69_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOG42361.1"
                     /translation="PSSTLSGGESQRIALVSSLGNNLVGSMYILDEPSIGLHPRDTRR
                     LISVLKKLRDLGNTVIVVEHDEDIIRAADQLIDIGPLAGKYGGEVVYQGPVTGENNSS
                     LTLKYLSERNLTSIPPARRRWNYSIEVVGAMQNNLKNINVRFPLKCLTAVTGVSGSGK
                     SSLVGDILHPALYRHIHQLGSKPGLFKELRGDLGRINSVEYIDQNPIGKSSRSNPVTY
                     LKVYDDIRKLFSEQPYAKMNNYGHSHFSFNIDGGRCPECQGEGVIKIEMQFMADVTIT
                     CEACGGKRFQPDILEVKFQGKNISDVLNMSVDEAIEFFSAQNDLTAKRIADKLRPLQD
                     VGLSYVSLGQSSSTLSGGESQRIKLASFLGKESASGSILFIFDEPTTGLHFQDIEKLL
                     KSFDALIEKGHSIIVVEHNLDIIRAADWVIDLGPDAGDKGGELVFEGTPEMLALHPEL
                     PTGAALAGVFRNQFPQ"
BASE COUNT          418 a          339 c          343 g          342 t
ORIGIN      
        1 ccctcttcta ccctcagcgg aggagaaagc cagcgcatcg cccttgtaag ctctctgggc
       61 aacaatctgg tgggatccat gtatattctg gacgagccga gcataggtct tcatcccagg
      121 gacacccgcc gccttatatc agtactgaaa aaactccgtg acctcggcaa caccgtgatt
      181 gtagtggaac acgacgaaga tatcatacgt gctgcagatc agctcatcga catcggaccg
      241 ctggcaggaa aatatggcgg cgaggttgtc tatcaagggc cggtcaccgg tgaaaataat
      301 tcatcactca cactcaaata cctctccgaa agaaatctaa cgagtatccc tcctgcgagg
      361 agaagatgga actactccat agaggtagta ggtgctatgc agaacaatct gaagaatatc
      421 aatgtccgct ttcctctaaa atgtctcact gcagtcacgg gtgtcagcgg cagcggcaaa
      481 tcttctctgg tgggagatat actgcatcct gcactttacc gccatattca ccagttggga
      541 agcaaaccag gactctttaa ggaactgagg ggagatctgg gcaggataaa ttcggtcgaa
      601 tatatagacc aaaatcctat cggcaagagt tcaaggtcca accccgtaac ctaccttaag
      661 gtctatgatg acatccgcaa gcttttttca gagcaaccct atgcaaaaat gaacaactac
      721 ggacactccc atttttcatt caatattgac ggaggacgct gtcctgaatg tcagggagaa
      781 ggagtgatca aaatagagat gcagtttatg gcagatgtaa ccatcacctg cgaggcatgc
      841 ggcggcaaaa ggttccaacc cgatattctc gaggtaaaat ttcagggtaa gaatatcagc
      901 gatgtgctaa atatgagcgt agatgaggcc atagagtttt tctcagctca aaacgacctt
      961 acggcaaaga ggattgccga caaacttcgg cccctccaag atgtcggact ttcctatgtc
     1021 agtctggggc agagcagcag tactctcagc gggggtgaaa gtcagagaat caaactggct
     1081 tcattcctcg gaaaggagtc cgcttcagga agtattctct ttatatttga tgaacctacc
     1141 accggacttc attttcagga tatagaaaaa ctgctcaagt catttgatgc tcttatcgaa
     1201 aaaggacact ctataattgt ggtagaacac aacctcgaca tcataagagc agccgactgg
     1261 gttatagatc tcggtcctga tgccggagac aaaggtggcg aactggtatt tgaaggcacg
     1321 ccggaaatgt tggctctcca tccggaactc cctacaggtg ctgctttggc aggagttttc
     1381 aggaatcagt ttccacaata gagactcttt gactatattt gtaatttaaa gaagatgaag
     1441 ac
//
LOCUS       DAOSIE010000333         1441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_78099_length_1441_cov_2.893218, whole genome shotgun sequence.
ACCESSION   DAOSIE010000333 DAOSIE010000000
VERSION     DAOSIE010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78099_length_1441_cov_2.893218"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLI69_07475"
     CDS             complement(<1..303)
                     /locus_tag="PLI69_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG42362.1"
                     /translation="MDRTWKEITDIALPAPRRKGGMPLMEALNRRESSRELTSKPLDL
                     QTLSDLLWAAWGYNRDHKRTAPSSHNRQEIDLYVFLESGTYIYDAAGNRLLLHDKDD"
     gene            complement(319..>1441)
                     /locus_tag="PLI69_07480"
     CDS             complement(319..>1441)
                     /locus_tag="PLI69_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOG42363.1"
                     /translation="WHYDIIGNSATNKEIIVASLAGELYFLDYSSGQPSRNTIDVFNV
                     IKGTPSLDPTLNGNLYVGQGVEINPPFGAMVIDLHKHAITHFVPKDPKAWRGWGAYDS
                     SPIRVGQFLFRLGENGTIYKYLVEQGTLSLHSTMRYRIKGKKAPGIESSPAIYRNYGY
                     FTDNSGNVICFNLNNLKPVWRYDNHDDNDATPVLCEEMGIPYLYTSCEVDKQDSIGFS
                     HFIKLNALTGEKVWENKIACQKAFSEKKWSDGGMFSTPLPGIGDCSEYIFASITTHIP
                     AFKGIFMAIDKSTGKTLYSIDLKRYAWSSPVPMLDPEGKMYIFAADCWGYIYLIEGAT
                     GEVLLTQKVGINFEGSPIIVDNAVIIGSRGDEIYKISIN"
BASE COUNT          343 a          319 c          357 g          422 t
ORIGIN      
        1 atcatcttta tcgtggagca gcagacggtt gcccgcagca tcatagatgt aggtaccgct
       61 ctcaaggaag acataaaggt cgatttcctg tcggttgtgt gatgagggag cagtgcgttt
      121 atgatctcgg ttgtaacccc aggcagccca gagcagatct gaaagtgttt gcaagtcaag
      181 tggctttgag gtcagttccc tggaggattc cctacgattg agagcctcca tcagaggcat
      241 tcctcctttg cggcgcggcg ccgggagcgc tatgtcagtt atctctttcc atgttctgtc
      301 cataatctat ctcttttatc aattaattga aatcttgtaa atctcatctc cacgggaacc
      361 gataatgacg gcattgtcta ctattatcgg agatccctcg aaattaatgc ccaccttttg
      421 tgtaagaagc acctctccgg tggccccttc aattagataa atatatcccc aacagtcagc
      481 tgcaaagata tacatctttc cttcgggatc cagcatcggc acgggagagg accaggcgta
      541 gcgtttgagg tcaatgctgt atagagtttt tcctgtgctt ttgtcaatgg ccataaaaat
      601 acccttgaat gccggtatat gtgtggttat agaggcgaaa atatattcgc tgcaatctcc
      661 aatgcccggt agtggagtgg agaacattcc tccgtcggac cattttttct ctgaaaatgc
      721 tttttggcag gcgattttgt tttcccaaac cttttcgccg gtcagggcat ttaattttat
      781 gaaatgggaa aatccaatgg aatcctgttt gtcaacctca caggatgtgt aaaggtaggg
      841 gatgcccatc tcctcgcaaa gcaccggtgt agcatcgttg tcatcgtggt tgtcatatct
      901 ccataccggc ttgagattgt tcagattgaa gcagatcacg ttgccgctgt tatccgtaaa
      961 gtagccgtag ttgcgataga tggcaggtga ggattcgatg ccgggggcct ttttgccctt
     1021 gattctatat cgcatagtgg agtggagact caatgttccc tgttccacaa ggtatttgta
     1081 tatcgtgccg ttttcaccca atctgaaaag aaattgccct acccttatgg gtgaagagtc
     1141 atatgcaccc catccgcgcc atgccttggg gtctttcggc acaaaatgag ttatggcatg
     1201 tttatgcagg tctattacca tagctccgaa aggaggattg atttctactc cctgtcccac
     1261 ataaaggttg ccgttgagtg tcgggtcaag tgaaggtgtg ccttttatga cattgaagac
     1321 atcgattgtg tttctggagg gttggccgga agaataatcc agaaagtaga gttcacctgc
     1381 gagcgaagcg acgatgattt ctttgtttgt ggcggaattg ccgatgatat catagtgcca
     1441 t
//
LOCUS       DAOSIE010000334         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_78750_length_1433_cov_3.236575, whole genome shotgun sequence.
ACCESSION   DAOSIE010000334 DAOSIE010000000
VERSION     DAOSIE010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78750_length_1433_cov_3.236575"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1433)
                     /locus_tag="PLI69_07485"
     CDS             complement(<1..>1433)
                     /locus_tag="PLI69_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOG42364.1"
                     /translation="ALKTADAGYLTRRLVDVSHDVIVTEDDCGTLRGLIARAIRDKDT
                     IVESLGERILGRTTVHDIFNPITGELILQAGEEICENEVALIDSLPIEQVEIRSVLTC
                     ESRKGVCAKCYGRNLATSRMVEKGEVVGVIAAQSIGEPGTQLTLRTFHVGGTAGGAVV
                     DSTVESKYDGTLHFEELRTIERINDNGDSETVVISRMTELRILDSNTGYAYSQYDIPY
                     GSILYKKEGDKVSKGDLICEWDPYNAITIVDTTGKIAFENMVEGVTFRKESSDEFALS
                     TDKVIIEAKDRTKNPTMLILGKDDKTLKQYNLPVGAHISVSEGDPVKVGDIVIKIPRA
                     IGKSSDITGGLPRVTELFEARNPSNPAVISEINGEVTMGKIKRGNREIIVKNRSGDEK
                     SYLIPLTKQILVQENDYVRAGSPLSDGGVSPSDILNVLGPVKAQEYIVNEVQAVYRLQ
                     GVKINDKHFEIIVRQMMRKVEITSPGDT"
BASE COUNT          325 a          368 c          343 g          397 t
ORIGIN      
        1 gtatcgcccg ggctggtgat ttccaccttg cgcatcatct ggcgtacaat aatctcgaaa
       61 tgcttgtcgt ttatcttcac gccttgcaga cgatataccg cctggacctc attcacaata
      121 tattcctgag ccttaaccgg accgagtaca ttaagaatat ccgaaggaga tacacctcca
      181 tcagagaggg gagagccggc tctcacataa tcattttcct gtaccaatat ctgcttggta
      241 agaggaataa gatagctctt ttcgtctccg ctcctgttct tgacgataat ctcgcggttg
      301 cctctcttga ttttgcccat tgtaacttca ccgttaatct cggagataac tgcgggattg
      361 gatgggttgc gggcctcaaa tagctcagta actcgcggca gaccgccggt gatgtcgctg
      421 gattttccga tagcgcgtgg aatcttgatg acaatgtcac cgactttcac cggatcaccc
      481 tcagaaacag aaatatgggc tcccacaggc aggttgtact gtttgagagt cttatcgtct
      541 tttcctaaaa tgagcatggt cgggttcttg gtacggtctt ttgcttctat aatcaccttg
      601 tcggtggaca gggcgaattc gtcactggat tctttacgga aggttactcc ttccaccata
      661 ttctcgaaag cgattttacc ggtggtatcc acaatcgtaa tcgcattata cggatcccat
      721 tcgcagataa gatctccctt acttaccttg tcgccttctt tcttgtacag gatagagccg
      781 taaggaatat catactggga atatgcgtag ccggtgttgc tgtcgagaat gcgcagctcg
      841 gtcatacggg aaatcactac ggtttcactg tcaccgttgt catttatgcg ctcgatggta
      901 cggagttctt cgaagtgaag tgttccatca tatttgcttt cgacagtaga gtccactaca
      961 gcaccgcctg cagtaccgcc gacgtggaag gtacgaagcg tgagctgagt accgggctca
     1021 ccgatggatt gggctgcgat aacacctaca acctctccct tttctaccat cctgctggtg
     1081 gcaagattac gtccatagca cttggcacaa actcccttgc ggctttcgca agtaagcacc
     1141 gaacggattt ccacctgctc gatggggaga gagtcaatga gtgcgacctc attttcacag
     1201 atttcctcgc ctgcctgcag gatgagttct cctgtgatag ggttgaagat atcgtgtacc
     1261 gtagtacggc caagaatcct ttccccgaga ctttccacta tggtgtcttt gtctctgatg
     1321 gctctggcta tgagaccgcg cagagtaccg cagtcgtctt cagtaacaat tacatcgtgc
     1381 gaaacgtcca ctaaacggcg ggtgaggtaa ccggcgtcgg ctgtcttcaa agc
//
LOCUS       DAOSIE010000335         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_78754_length_1433_cov_3.048621, whole genome shotgun sequence.
ACCESSION   DAOSIE010000335 DAOSIE010000000
VERSION     DAOSIE010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78754_length_1433_cov_3.048621"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PLI69_07490"
     CDS             <1..633
                     /locus_tag="PLI69_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOG42365.1"
                     /translation="PGVVTRKYGTTRYEVSLIGRDEDSPYLSLKVHDQIIDTNLIGTY
                     NVDNILAAIAIGEYFEVPFSNARKAIESYSPSNHRSQMLQTPFNTLILDAYNANPTSM
                     RASLDNFIQLPLERKWVILGDMLELGDDSQEEHKGIVEFLQKQRSLEHIVLVGREFGK
                     AASELRSDRLQLFTSVEKLKEYFEKHRPEGASIFIKGSNGIRLSLLEEVL"
     gene            650..>1433
                     /locus_tag="PLI69_07495"
     CDS             650..>1433
                     /locus_tag="PLI69_07495"
                     /inference="COORDINATES: protein motif:HMM:NF037611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42366.1"
                     /translation="MRRFFIAALSILIPLGCAAGIFDRGKVSYIKYVNPFVGTDFHGH
                     TFPGAAYPFGMIQLSPDTRTDNWDGCSGYHYSDSTIWGFSHTHLSGTGCADYCDLLVM
                     PVVDYQAFVTAMEQEAGREMGDFPHDYIFNEYYESPFSHLDEKASPGYYGVYLERWRV
                     QADLSVGRRSAMHKYMFPKGVEPQLVIDLEHRDKVLDSHIKQEGHNAISGRRRSQSWA
                     QDQDLYFYMEFSRPIKFAQIVGEDGYGAKALITFKKWFFSREL"
BASE COUNT          371 a          313 c          371 g          378 t
ORIGIN      
        1 ccgggggtgg ttacccgtaa atatggtact acccgctatg aggtctcctt gatcggtagg
       61 gatgaagact cgccctatct gagtttgaag gtgcacgatc aaataattga taccaatctg
      121 atcggtactt acaatgtgga taatattctg gcagccatag ctatagggga atacttcgaa
      181 gttcctttca gcaatgcccg gaaggccatc gaaagctact ctccctccaa tcatcgttca
      241 cagatgctcc aaactccctt caacaccctc attttggatg cctataacgc aaatcccaca
      301 agtatgcgcg cctccctgga taatttcatt caacttcctt tggagcgcaa atgggtaata
      361 ctcggagata tgctcgagct tggagacgac tctcaggaag agcacaaagg catagtggag
      421 ttccttcaaa aacagagatc tttagagcat atagtgctgg tggggcggga atttggcaaa
      481 gcagcgtcag aactgagatc tgataggttg cagttgttca cgagtgtgga gaagcttaag
      541 gaatactttg aaaagcaccg tccggaggga gcctcgatct ttatcaaggg ttccaacggt
      601 atcaggctct ccctgcttga agaagtttta taaaatatat tagacctcta tgcgtagatt
      661 tttcatagca gcgttgtcga tattgatccc gctgggatgt gctgcaggta tatttgaccg
      721 tggtaaggtt tcatatataa aatatgtaaa tccattcgta ggaacggatt tccacggaca
      781 tacatttccc ggggcggcct atcctttcgg catgatacag cttagccccg atacgcgtac
      841 cgacaattgg gatggttgct ccggttatca ctattcggat agcaccatct ggggtttttc
      901 gcatacgcac cttagcggaa ccggatgcgc cgattattgc gatctgctgg taatgccggt
      961 tgtcgactat caagcctttg tgacagctat ggagcaggaa gccggccgtg agatggggga
     1021 ttttccgcat gattatattt tcaatgaata ttatgagtcg cctttcagcc atttagatga
     1081 gaaggcctct ccgggctatt acggagtcta tctggagcgc tggagagtgc aggctgatct
     1141 gagtgtgggc aggcgttctg cgatgcacaa atatatgttc ccaaagggag tcgaaccgca
     1201 gttggtgata gatttggagc atagggacaa ggttctcgat tcccatataa agcaggaagg
     1261 gcacaatgcc atcagcggca ggcgcaggag ccaatcctgg gctcaggatc aggacctcta
     1321 tttctatatg gaattcagcc gtccgataaa gtttgcgcag attgtcggcg aagatggata
     1381 tggagccaag gcgctaatta cattcaaaaa atggtttttt agcagggaac tct
//
LOCUS       DAOSIE010000336         1419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_79894_length_1419_cov_2.484604, whole genome shotgun sequence.
ACCESSION   DAOSIE010000336 DAOSIE010000000
VERSION     DAOSIE010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79894_length_1419_cov_2.484604"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1419
                     /locus_tag="PLI69_07500"
     CDS             <1..>1419
                     /locus_tag="PLI69_07500"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOG42367.1"
                     /translation="PVKTIDITNNKGHIVIKPTIEGNYSYYIQGITENGSVEIGGKNR
                     INLTLNGTGITNPNGAAISILTEGGCQLRVEDNTANRLIDGTVVTSTAKATLICEGPL
                     DIVGKGTLQLRGKKADAIRCGGSITIDQASILVKEAAGHSIHCTSGEIKIAGGAIKTL
                     SSQSGLNASDSNNGSITITGGIHRFSTLGDQAPAIKAAGSLALANRDTIITDTYGNAA
                     HSIYVSGKTLISNSILCTYTLGSHIPVCIYGGDDINITGGNISHITVNGSGIFCKGDI
                     TINGGTISAISTAAGTAILTSEGKFSISDGSIAIYTESAYNMAIYSKGPLSIRGGKVE
                     LTSFNTSMGSASTLEISGGNVYCYSLSGYAIHSDGNLTLSGGLTLALGADGAGKALNT
                     KSSILIRGGHLVAVGGKSSIPDASGSTQQSVIYNTTLYEPAIIAVKQINGQFIAVYKA
                     TRKYQPTISCLISGPEILPNTT"
BASE COUNT          432 a          341 c          309 g          337 t
ORIGIN      
        1 tcctgtcaaa actattgata tcaccaataa taaggggcat attgtcataa aacccacaat
       61 tgaaggcaat tactcctact acattcaggg gataactgaa aacggttccg tagaaatagg
      121 gggtaaaaac cgcataaacc tcaccctgaa tggcacaggt attaccaacc cgaatggcgc
      181 tgcaatctcc attctcacgg aaggggggtg ccaattgaga gttgaagaca acaccgccaa
      241 tcgtttgatt gacggcaccg tggtcacaag cactgcgaaa gccactctaa tatgtgaagg
      301 ccctcttgat atcgttggca agggcacttt gcaactaaga ggtaaaaagg cagatgccat
      361 ccgttgcggc ggctccataa ccatcgacca ggcttccata cttgtaaaag aggctgccgg
      421 acactctatt cattgtactt cgggagagat aaagattgcc ggaggagcta ttaaaacact
      481 ctcatcgcaa agcggactta atgcatcgga tagcaataac ggatcaataa caattaccgg
      541 cggtattcat agattttcaa cacttggcga tcaggctccc gcaattaaag cagccggaag
      601 tcttgcctta gcgaataggg acactatcat taccgatact tacggcaatg ccgcccatag
      661 catatatgtt tcaggtaaaa cattgataag caactccata ctatgtacct acacgctcgg
      721 atctcatatt ccggtctgta tatatggtgg tgatgacatc aatattaccg gtggtaatat
      781 ctcacatata accgtaaacg gttcaggtat cttttgtaaa ggcgacatta caattaacgg
      841 agggactata agtgccatta gtactgctgc ggggacagca atacttacaa gtgaaggtaa
      901 attcagcatc agcgacggtt ctattgcaat ctataccgaa tctgcctaca atatggctat
      961 ctactccaaa ggccccctct ccatcagagg aggtaaggtt gaattgacct cattcaacac
     1021 ctccatggga agtgcttcca cattggaaat aagcggcgga aatgtttatt gctatagtct
     1081 atccggttat gcaatacata gcgacggcaa tcttacccta tcaggaggac tgactctggc
     1141 tctgggagct gacggagccg gcaaagcact aaacaccaaa tcctcaatac tgatcagggg
     1201 gggacatctg gtagctgtag gcggaaagag cagtatcccg gatgcatccg gttctaccca
     1261 gcagtcagtg atctacaaca ccacgctcta cgagcctgcc atcatagcgg taaaacaaat
     1321 taacggtcaa ttcatagccg tatacaaggc taccagaaaa taccaaccga ctatcagttg
     1381 tcttatttcc ggaccggaaa ttcttcccaa tactacgta
//
LOCUS       DAOSIE010000337         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_80308_length_1414_cov_2.409124, whole genome shotgun sequence.
ACCESSION   DAOSIE010000337 DAOSIE010000000
VERSION     DAOSIE010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80308_length_1414_cov_2.409124"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PLI69_07505"
     CDS             complement(<1..1175)
                     /locus_tag="PLI69_07505"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOG42368.1"
                     /translation="MKRTLLTIALLLLAIKGFTCTNLIITKGASTDGSVMVTYAADSH
                     TRYGTLVHYPAAKYKAGTLLPVYEWGKDRYLGTIPQAEKTYNVIGNMNEHQLIIGEST
                     WGGLDQLRDPNGILDYGSLMYITLQRARTAREAILLYTSLADQYGYASSGESISFADP
                     EEAWILEIIARKPEVVNGVNTRKGAVWVAVRIPDGYISAHANCARITTFPLNDPENCL
                     FSPDVISHARELGLFEGKDEEFSFADTYCPLDFGAMRGCEARVWSFFNKHGAEDMSKY
                     IDHASGHNPENRLPLFVKAKHKLSVTMVADMMRDHFEDTPFDMRNDIGAGGNTLPYRW
                     RPMSFEIDGEEYINERAIATQQTGFWFVGQARNWLPDEIGGIFWFAVDDAGTSPLTPV
                     "
     gene            complement(1185..>1414)
                     /locus_tag="PLI69_07510"
     CDS             complement(1185..>1414)
                     /locus_tag="PLI69_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42369.1"
                     /translation="LTDTSLFVINTVKRTPHISHYSLPEAVEVAGRVGAKRSFITHLS
                     HQLPRHAQLKQELHEQPFSIEPAYDGLTIEF"
BASE COUNT          347 a          328 c          349 g          390 t
ORIGIN      
        1 acgggagtca gaggtgaagt gcccgcatca tctacggcaa accagaagat tccccctatc
       61 tcatcgggga gccagttacg cgcttgtccg acaaaccaaa atcccgtctg ttgagtagca
      121 attgctcttt cattgatata ctcttcaccg tcgatttcaa agctcattgg tctccaacgg
      181 tagggaagag tgtttccccc tgctccgata tcattgcgca tatcgaaggg cgtatcttca
      241 aaatggtccc tcatcatatc ggcaaccatg gtaacagaga gtttgtgctt tgccttgaca
      301 aaaagcggaa gcctgttttc cggattatgc cccgaagcat gatcaatata tttggacata
      361 tcttcagcac catgtttatt aaaaaaactc cagactctcg cctcgcatcc tctcatggcg
      421 ccaaaatcca gaggacaata ggtatcggca aaactgaatt cctcatcctt accttcaaat
      481 agccccaact ccctcgcatg tgagattaca tcgggtgaga agaggcaatt ttccggatca
      541 ttaagtggaa atgtggtaat acgtgcacag tttgcatggg ctgagatata gccgtcaggt
      601 atacgtacag ccacccagac tgctccttta cgggtattga cgccatttac gacttcgggc
      661 ttgcgtgcga tgatttccaa tatccaggcc tcctcagggt cagcaaagga gatggactct
      721 cctgatgagg cataaccata ttggtcggca agcgaggtat aaagcagtat cgcctcacgt
      781 gcagtccgtg ccctttgcag ggtaatatac ataagtgatc cgtagtccag tatgccgttg
      841 gggtccctga gttgatccaa acctccccag gttgattcgc ctatgataag ctgatgctca
      901 ttcatgttgc cgatgacgtt ataagtcttt tctgcctgag gtatggtgcc gaggtagcga
      961 tccttgcccc attcatatac ggggagcagt gtgcctgcct tgtattttgc tgccggatag
     1021 tgtaccagcg ttccatatct ggtgtgactg tcagccgcgt atgtgaccat tacggaaccg
     1081 tcggtagaag cgccttttgt aatgataagg ttggtgcaag taaaaccttt gattgctaat
     1141 agcagaagag ctattgtaag tagtgtacgt ttcataatct attatcaaaa ttcaattgtg
     1201 agcccgtcgt atgcaggttc tatgctgaat ggttgttcgt ggagttcttg ttttaattgt
     1261 gcatgtctag gtaactggtg ggagagatgt gtgataaagg agcgctttgc tccaaccctg
     1321 ccggccacct ccacagcctc ggggagcgag taatgggaga tatgaggtgt gcgtttgacc
     1381 gtattgatga caaagagcga tgtgtctgta agtt
//
LOCUS       DAOSIE010000338         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_80958_length_1406_cov_2.133975, whole genome shotgun sequence.
ACCESSION   DAOSIE010000338 DAOSIE010000000
VERSION     DAOSIE010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80958_length_1406_cov_2.133975"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLI69_07515"
     CDS             complement(<1..232)
                     /locus_tag="PLI69_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG42370.1"
                     /translation="MLVGESGVGKTAIVEGLASTLEGKRIFSLDIPALIAANPHPSSL
                     AESIRQIIAAIEATGNAILFIDDIHALVGTGGM"
     gene            complement(223..828)
                     /locus_tag="PLI69_07520"
     CDS             complement(223..828)
                     /locus_tag="PLI69_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOG42371.1"
                     /translation="MKNTLSRELSALLERAREEAMRMGSTAIDADHLFLAILRDSKRL
                     VNSISDRIGSDISIVKTALEETISQPEPIPFESIGSVTISQEVNDIFGEAFAGGRAAN
                     AQPGVAEILESAITLRKGRFIPLMEKIGILPDTVLSAIEEISRRSDRIEKKTTYILVK
                     YCRDLKSVSDSENCDPLKGRNPKYCVLNRYSTVAKRIIPCW"
     gene            940..>1406
                     /locus_tag="PLI69_07525"
     CDS             940..>1406
                     /locus_tag="PLI69_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOG42372.1"
                     /translation="MEELEENVGRIIPINIETEMKSAYIDYSMSVIVSRALPDVRDGL
                     KPVHRRVLYGMYGLGLTSNQSVKKSARIVGEVLGKYHPHGDSSVYDAMARLAQSWNMR
                     YMLVHGQGNFGSVDGDPVAAMRYTEAKLQKLAEEIIEDIDKDTVDFKPNFDESL"
BASE COUNT          359 a          289 c          340 g          418 t
ORIGIN      
        1 gcattccacc tgtcccaacc aaggcatgga tatcatctat gaagaggatt gcgtttccgg
       61 tagcctctat agctgctatt atttgtctta tgctttctgc aagcgaggaa gggtggggat
      121 ttgctgcaat aagtgccggg atatcgagag agaagatcct tttgccctca agtgttgagg
      181 cgagtccctc aacaatagcg gttttaccta ccccggattc acctaccagc aagggattat
      241 tctttttgcg acggttgagt atctgtttaa cacgcagtat ttcggattcc ttcccttgag
      301 aggatcgcaa ttttcactat cggagactga tttaagatcc cggcagtatt ttaccagtat
      361 gtatgtcgtt tttttctcta tacggtctga tctacgtgat atctcttcaa ttgcagaaag
      421 cacagtatcg ggcagtatgc caattttttc cattagggga atgaatcttc ctttacgaag
      481 ggtgattgcg gattccaata tctctgctac gccgggttgt gcattggcag cgcgacctcc
      541 ggcaaatgct tctccaaaaa tgtcattaac ttcctgtgaa atggtcactg aaccgatact
      601 ttcaaaggga attggctccg gctgggaaat tgtctcttcc agggccgttt taactattga
      661 aatgtcactt ccaatgcggt cagagatgga attgacaagc ctcttgctat ccctgagaat
      721 tgccagaaag aggtggtcgg catcaatggc tgtgctaccc attctcattg cctcttcgcg
      781 ggctctctcc agcagggctg aaagttctct ggaaagggta tttttcataa tcttgtagtg
      841 ctttatttca aagtgccaag atagtgaaaa attttggccg aatcactttt tctttgtact
      901 tttacgagtt aatttttaca taaagtagaa agaaaaggaa tggaagaatt agaagagaat
      961 gtaggtagga ttattcctat caatattgaa actgagatga agagcgccta tatcgattat
     1021 tcgatgtcgg tgattgtgtc gcgtgcgctt ccggatgtga gagatggtct gaaacctgta
     1081 cacaggagag ttctatacgg catgtacggt ctcggcctga ccagcaacca atctgtaaag
     1141 aaatcagctc gtatagtggg tgaggtgctt ggtaaatatc atccccacgg tgattccagc
     1201 gtttatgacg ctatggcaag acttgcccag tcctggaata tgcgctatat gcttgttcac
     1261 ggccagggta acttcggatc cgttgacggc gacccggtag cggcgatgcg ttatacggag
     1321 gcaaagcttc agaaacttgc tgaagagatt atagaagata ttgataagga tacggttgat
     1381 ttcaaaccta attttgatga gtcact
//
LOCUS       DAOSIE010000339         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_81473_length_1399_cov_3.591518, whole genome shotgun sequence.
ACCESSION   DAOSIE010000339 DAOSIE010000000
VERSION     DAOSIE010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81473_length_1399_cov_3.591518"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLI69_07530"
     CDS             <1..399
                     /locus_tag="PLI69_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOG42373.1"
                     /translation="CRAVMHTHPTELVAMSHYAPFLEKDVLGTLLRSLIPETYIFCPK
                     GLGIVPYALPGSQKLAEKTLEKLRSHDVVLWEKHGVLAVGKDPVEAFDMIDILNKSAQ
                     IYLRARAMGFVPAGLTPEQMEELKKNFIPF"
     gene            409..951
                     /locus_tag="PLI69_07535"
     CDS             409..951
                     /locus_tag="PLI69_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOG42374.1"
                     /translation="MLWELFWTFFKLGTFTFGSGYAMIPMIEREVVDRKKWFEREDFY
                     NQFTLAQSAPGPFALNTAVFVGYKMKGWWGSLVSVIGVSISPFVIILLIAVYLSGFST
                     NPIVEAAFKGIRPAVIAMIAVPFINMMKGLRWYFIALAIGVAVVVWLTGISPIWLLLA
                     GAAVGVSVALSGKNEGEVKQ"
     gene            948..>1399
                     /locus_tag="PLI69_07540"
     CDS             948..>1399
                     /locus_tag="PLI69_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOG42375.1"
                     /translation="MIYLQLFLSYLKIGFFGFGGGYAMISLIQNEVVIRNNWLTSLEL
                     ADIVAISQMTPGPIAINCATYVGYTVTGNIWGSLLATFAVCLPPLTIMTLITIFYSRL
                     HNNYIVKSTLSWMRPIMTGMIGAAALMFLNRDTFVDWVSIVIFAVSFFA"
BASE COUNT          344 a          290 c          352 g          413 t
ORIGIN      
        1 tgcagggcag tgatgcacac tcatcccacg gaactcgtgg caatgtccca ctacgcgcca
       61 ttcctggaaa aagatgtgct gggcactctc ttgcgcagtc ttatccccga gacctatatc
      121 ttttgtccaa aagggctggg aatagtgcct tatgcgctcc cgggctcgca aaagttggca
      181 gagaagactc ttgaaaagtt gcgttcacac gatgtggtgc tttgggagaa gcatggtgtg
      241 ctggcagtgg ggaaggaccc cgttgaggca tttgacatga ttgatattct caataagtcg
      301 gcacaaatat atttgagagc acgtgcgatg ggttttgtac ccgccggact cactccggag
      361 cagatggagg aactcaaaaa gaattttatc ccattctaag cggcgactat gctttgggag
      421 ttgttttgga cattctttaa actgggtacc tttacattcg gtagcggcta cgccatgata
      481 cctatgatag aaagggaagt tgtcgaccgt aagaagtggt tcgagaggga ggatttctat
      541 aatcaattca ctcttgcaca gtcggcaccc ggacctttcg cgctaaatac tgccgtattc
      601 gtcggctata agatgaaggg atggtggggt tctttggtat ccgtaatagg tgtttcaatc
      661 tctccatttg tgattattct gcttatcgcg gtttatttga gtggattcag cacgaatcct
      721 attgttgagg cagcctttaa gggtataaga cctgcggtaa tagcaatgat agctgtcccc
      781 tttatcaata tgatgaaagg tctcagatgg tattttatcg cattagccat cggtgttgcc
      841 gttgtggtgt ggttgaccgg tatttctccg atatggttac ttctggcagg agctgctgta
      901 ggtgtatcgg tcgcgcttag cggcaaaaat gaaggggagg tgaagcaatg atctatcttc
      961 aactcttttt atcctatctg aaaataggat ttttcggttt tggaggcgga tatgccatga
     1021 tttcgcttat acaaaatgag gttgtgatac gcaacaactg gctcacatcc ctggaacttg
     1081 cagatatagt agcaatctca caaatgactc ccggcccgat tgcaataaac tgtgctactt
     1141 atgtgggtta taccgttaca ggaaatatat ggggatcact tttagccaca tttgccgtct
     1201 gtctgccgcc tctcaccata atgacgttaa tcactatctt ttatagtcgg ctacacaata
     1261 actacattgt caaaagtacc ctctcatgga tgagacccat aatgacggga atgatcggtg
     1321 ctgctgctct aatgtttctc aatagggata catttgtgga ttgggtgagc atagtgatct
     1381 ttgcagtaag tttcttcgc
//
LOCUS       DAOSIE010000340         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_82223_length_1390_cov_3.113109, whole genome shotgun sequence.
ACCESSION   DAOSIE010000340 DAOSIE010000000
VERSION     DAOSIE010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82223_length_1390_cov_3.113109"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLI69_07545"
     CDS             complement(<1..706)
                     /locus_tag="PLI69_07545"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOG42376.1"
                     /translation="MKKIFFALLSVILFLFSAGATAQTKGYPFQSGENMELIIHYKWG
                     FSADIASVKFTFTEKSEEGKEPYYHLLAHASTYKFWDSFFKVRDIYESKFYVKDLSPL
                     YFHRDVSEGNFYAKNWYSWQDGGKTLNAIVDKKGRPRRDTTYYENDVIRDIINIFFTV
                     RCLDFAKLEKGKPAPFIIALDKDILDMRVRFVGREKKRVPKVGTFNAIKLAIAVNPRK
                     GRTKDGETSVFSFGEGD"
     gene            complement(720..>1390)
                     /locus_tag="PLI69_07550"
     CDS             complement(720..>1390)
                     /locus_tag="PLI69_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOG42377.1"
                     /translation="DKIRGARKMTSAEWGQLNEGVARLSKSPLWIDDTPSLSIYEFRS
                     KARRLVNNNKVKIIIIDYLQLMTGPPDLRGMREQEVSAISRSLKAIAKELNVPIIALS
                     QLNRSVETRGGNKRPQLSDLRESGAIEQDADIVMFIHRPEKVGLPSDDLIAGGTEIII
                     LKHRNGAVGDVPMKFESDRVRFVDIDDVLPGDPMIRTEYHGSKMNSAEPLDYAGLGPS
                     DDFN"
BASE COUNT          369 a          340 c          297 g          384 t
ORIGIN      
        1 catcaccttc tccaaatgag aatacggacg tctccccatc ttttgtacga ccttttctgg
       61 ggtttaccgc aatagccagc tttatggcgt tgaaagtacc cactttaggc actctcttct
      121 tttctcggcc tacaaaacgt acccgcatat cgaggatatc tttgtcaagg gctatgataa
      181 agggtgccgg ctttcctttt tcgagctttg cgaaatccag acacctcacc gtaaaaaaga
      241 tattgataat atcacggatc acatcatttt catagtatgt ggtatccctg cgggggcgac
      301 ctttcttgtc aactatggca ttgagcgtct tgccgccatc ttgccagcta taccagttct
      361 tggcataaaa gttaccttcg ctcacatctc tgtgaaaata gaggggagag aggtccttga
      421 cgtagaattt ggattcataa atgtccctta ccttgaaaaa ggagtcccaa aatttatagg
      481 tggaggcatg ggccagcaga tgataatagg gctctttacc ctcctcggac ttttcggtaa
      541 aggtaaactt cacactggca atatccgcac tgaaacccca tttgtaatgg atgatcaatt
      601 ccatgttttc accgctttgg aaaggataac cctttgtctg ggcggtagcg cctgccgaaa
      661 ataaaaatag aataaccgac agtagcgcaa aaaatatctt tttcatcaaa ataatcgttt
      721 taattaaaat catcactcgg acccaatccc gcataatcca gaggctccgc actattcatc
      781 ttcgaaccgt gatattcggt acggatcatc ggatcaccgg gcaatacgtc atctatatcc
      841 acaaatcgta ccctatccga ctcaaacttc atcggaacat ctccaaccgc accgtttcta
      901 tgttttagaa tgatgatttc ggtacctccg gcaatcaaat catcggaagg aagacctact
      961 ttttcgggac gatggataaa cattacgatg tccgcatcct gctcaatggc tcctgactca
     1021 cgaaggtcgc tcagttgggg acgcttattg ccgccacgcg tctccacgga acggttgagc
     1081 tgcgaaagag ctattatggg aacattgagt tcctttgcaa tcgcttttaa agagcgggaa
     1141 atggcagaga cctcctgttc acgcataccg cgaaggtcag ggggtccggt catcagttga
     1201 agatagtcta tgataatgat ttttacttta ttgttgttga caaggcgccg tgccttggag
     1261 cgaaactcat aaatggagag tgaaggagta tcatcaatcc acaatggaga cttggagagt
     1321 cttgcaactc cctcgttgag ttgaccccac tctgcactag tcatctttct cgctccacgg
     1381 atcttatcgg
//
LOCUS       DAOSIE010000341         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_83684_length_1373_cov_2.993930, whole genome shotgun sequence.
ACCESSION   DAOSIE010000341 DAOSIE010000000
VERSION     DAOSIE010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83684_length_1373_cov_2.993930"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLI69_07555"
     CDS             complement(<1..231)
                     /locus_tag="PLI69_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOG42378.1"
                     /translation="MTYLQLSDEIRRKGSFLCVGLDTDIRLIPKHLQNCEYPLFDFNK
                     GIIEATAPYAVAFKPNTAFYEALGFFGWKQLEM"
     gene            complement(237..>1373)
                     /locus_tag="PLI69_07560"
     CDS             complement(237..>1373)
                     /locus_tag="PLI69_07560"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOG42379.1"
                     /translation="DPAKPTVLIYGHYDVQPVDPLELWHTPPFEPDIRNGAIYARGAN
                     DDKGQSFMHIKAFEYLVREGKLRHNIKFLLEGEEEIGSPSLGKWVRDHKKMLACDIIL
                     VSDTTMISEKVPSINCGMRGLAYLEVEVTGPDKDLHSGHYGGAVVNPINALCSMIDSL
                     IDDDGRVTVKGFYDDVVNLSRKDRELLGKAPFDKKEYMDSIGVTALRGEKGYTTLERT
                     GIRPCLDVNGIWGGFTGEGAKTVIPSTAYAKISMRLVPDQEYGKISKLFAKHFKSIAP
                     KGVKVKVTEHHGGNGFLIPISSDAYAAASKAITQVYGIEPVPSRGGGSIAILAEMQQA
                     LKADPLLMGFGLERDTIHSPNESFLISQFFKGMRSIALFYENFR"
BASE COUNT          332 a          325 c          331 g          385 t
ORIGIN      
        1 catttccagc tgtttccatc caaaaaaacc taatgcctcg tagaaggccg tatttggctt
       61 gaatgctact gcgtagggag cggtagcttc gattattccc ttgttgaaat caaacagggg
      121 atattcgcag ttttgcaagt gcttagggat tagtctgata tcggtatcaa gtccgacaca
      181 aaggaaactt cctttgcgtc ggatctcgtc gctgagttgt aaatatgtca tataaactac
      241 ctgaagtttt cgtagaagag agctatcgaa cgcattcctt tgaaaaattg ggaaatgagg
      301 aaactctcgt ttggtgagtg gatagtatca cgttccagtc caaatcccat cagcaggggg
      361 tcagccttaa gggcttgctg catttcagcc aggatggcga tgctaccacc acctcggctg
      421 ggtaccggtt caatcccata gacctgtgtt atggcttttg aagctgcggc gtatgcatcc
      481 gaggagatgg gaataaggaa tccgttgcct ccgtgatgct ccgtaacctt aactttcact
      541 cctttagggg caattgactt gaagtgcttc gcaaagagtt tggatatctt gccatactcc
      601 tggtcaggta cgagcctcat agatatcttt gcgtaggcgg tagagggaat aacggtcttt
      661 gctccctctc cggtaaagcc accccagata ccgtttacat cgagacaggg ccttataccc
      721 gtacgctcga gagtggtgta ccctttttca cctctaagcg ctgtgacgcc gatagagtcc
      781 atatattctt ttttgtcaaa aggagcctta cctagcaatt cgcggtcctt tcgactaaga
      841 tttaccacat catcatagaa tccctttaca gtgacgcgtc catcatcatc gatgagcgag
      901 tcaatcatgg agcagagggc gtttatgggg ttgactactg caccgccata gtgaccggag
      961 tgaagatctt tgtcaggtcc tgtaacttcc acctccagat atgcaaggcc tctcattcca
     1021 caattgatcg aaggcacttt ttccgatatc atagtggtgt ctgagacaag gattatatcg
     1081 cacgcgagca tctttttgtg atcacgcacc catttcccta gggagggtga gccgatctct
     1141 tcttctcctt caagaaggaa tttgatatta tggcgaagtt taccctctct taccaggtat
     1201 tcaaacgcct taatatgcat gaacgactgc cctttatcat catttgctcc gcgggcgtaa
     1261 atggctccgt tgcggatgtc cggttcaaag ggtggtgtgt gccaaagttc caaagggtct
     1321 acaggctgca catcatagtg accatagatg agtaccgtgg gtttggcagg gtc
//
LOCUS       DAOSIE010000342         1362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_84717_length_1362_cov_2.491201, whole genome shotgun sequence.
ACCESSION   DAOSIE010000342 DAOSIE010000000
VERSION     DAOSIE010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84717_length_1362_cov_2.491201"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLI69_07565"
     CDS             <1..359
                     /locus_tag="PLI69_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42380.1"
                     /translation="ALIPGFPLFLVMGAASLPIWVVSEVLCTLFKDRAFHNSVRMVVF
                     WVGWSLLYLIEVVLLFCFAPWQWALVAAVILMPAPFFVYDFFEFVRVLASLWRIVFNR
                     RLRNDYKEIKNELNKI"
     gene            372..>1362
                     /locus_tag="PLI69_07570"
     CDS             372..>1362
                     /locus_tag="PLI69_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOG42381.1"
                     /translation="MKRFIITLVLAGIVFPNLSYGQTVDTLRADKTQDTLRLEPYGKK
                     KPEPEVLVAPPDTTVKSVEAGELAKGDIIKTGLSFGPLPVVAFDNDKGFQYGALLNIY
                     NFGDGSWYPVPKSQWYLELSRFTKGSQLYVISYDNRTLIPGVRLSTALHVTRDKALDF
                     YGFNGYESFYDGYAQTGFYRMDRMVHTFKVDFTGKIKGNLYWKAGYHFSHFKTGAFTN
                     EEYTLPTSLFEIYKNYGIIPKEQADGGISSAIRMGLMYDSRDFEAAPSRGIWAEANLA
                     YAPEFLGTSVGYTKYNLTFRNYLPIAKDKLVFAYRLNLQGFIGKESPAFYVLPY"
BASE COUNT          348 a          318 c          307 g          389 t
ORIGIN      
        1 tggctcttat acccggtttt cccctctttt tagtgatggg agccgcatct ctgcctatat
       61 gggtcgtatc tgaggtgctc tgtaccctat ttaaggaccg cgcattccac aattcggtac
      121 gtatggtagt cttttgggta ggctggagcc tcttatattt gatcgaagta gtgctgctat
      181 tctgtttcgc cccatggcag tgggctcttg tcgccgctgt catactgatg cccgcaccgt
      241 tcttcgtata tgactttttt gagtttgtgc gtgtgctggc ctccctctgg agaattgttt
      301 tcaaccgcag gttgagaaat gattataaag aaattaaaaa tgaattgaac aagatctgat
      361 attgaataat tatgaaacga tttataataa ccctggtatt ggcgggaata gttttcccca
      421 acctgtcata cgggcaaacc gtagatacct tacgggctga taagactcag gacacactgc
      481 gtctggaacc ctacggtaag aaaaaaccgg aacccgaagt gctggtggca cccccggaca
      541 ctaccgtaaa gagtgtcgaa gccggtgaac tggccaaagg cgatatcatc aagaccggac
      601 tttcatttgg tccgctaccc gtagttgcct tcgacaacga taagggattt cagtatggag
      661 ctctgctcaa tatctacaat ttcggggatg gatcgtggta tcccgtaccc aaatcgcaat
      721 ggtatttaga gctttcgaga tttacgaaag gctcccaact ctatgtaatc tcatacgaca
      781 atcgtacgtt gattcccgga gtacgtcttt ctaccgctct ccatgtaaca agggacaaag
      841 cgctcgactt ttacggtttc aatggttatg agtctttcta tgatggctat gcacaaaccg
      901 gattttaccg tatggaccgt atggtgcata ctttcaaagt agactttacc ggaaaaataa
      961 agggcaacct gtactggaag gccggttatc atttcagcca cttcaagacc ggtgccttca
     1021 ctaatgaaga gtacactctt ccaacctccc tttttgaaat ttataagaat tacgggatta
     1081 tacccaagga gcaggctgac ggcggtattt caagtgccat cagaatggga ttgatgtatg
     1141 actcgcgtga cttcgaggcg gctccctcca gaggtatctg ggcagaagcc aatctcgctt
     1201 acgcccccga atttttaggc acttcagtag gttataccaa gtacaatctc actttcagga
     1261 attatctccc cattgcaaaa gacaaacttg tttttgccta cagattgaat ttacaaggct
     1321 ttatcggtaa agagtcacct gcattttacg tactcccgta cg
//
LOCUS       DAOSIE010000343         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_87363_length_1334_cov_2.793589, whole genome shotgun sequence.
ACCESSION   DAOSIE010000343 DAOSIE010000000
VERSION     DAOSIE010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87363_length_1334_cov_2.793589"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1161
                     /locus_tag="PLI69_07575"
     CDS             58..1161
                     /locus_tag="PLI69_07575"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOG42382.1"
                     /translation="MFSFRTTPLIEQSDRVLRHGRLGMLCNQVAWHPDKGEYHFETLA
                     RRGNLVRLFMPEHSLYGECVPGVEVVEIADSIPMEQLQDIDALIIELRDVGSRYCTAT
                     RTLLKLFYDLHNSGSDISIYVIDGINSSGRQVEGTMGLMGLPHRHGLTFGEIANFFYY
                     ELNANFHLHIISAEAEQVNKYLMPWSIPPFSDFAGLFTSHFYSGQCLWMGTNVSYGHG
                     TTRPFEQFGAPFMEPLYDFNRENGYKNWNDPANLIYDDSLYIRWCRFEPAYGIWKDEV
                     CFGFHLMFIPGHSYHALKHALRLIRFVREQFPEDFRFDGLEPFLQDNMLFSYIAGEID
                     WELTSEHIKTEEQKWLRKVKKYLLYGDSPWRVK"
     gene            1169..>1334
                     /locus_tag="PLI69_07580"
     CDS             1169..>1334
                     /locus_tag="PLI69_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HOG42383.1"
                     /translation="MENKVLKILFVCLGNICRSPMAEFIMKHKVEQLGVADRFEIASA
                     GTSDEERGNPV"
BASE COUNT          332 a          284 c          321 g          397 t
ORIGIN      
        1 aagtaatctc cttactctcc taaccccgca acccgcaacc cgtaacccgc accccgcatg
       61 ttttcatttc gtacaacacc acttattgaa caatccgatc gcgtacttag acacggcagg
      121 ctgggcatgc tttgcaacca ggtggcctgg catcccgata aaggggagta tcatttcgag
      181 actcttgccc gtaggggaaa tctcgtgcgt ctctttatgc cggagcattc tttgtatggc
      241 gagtgtgtcc cgggtgttga ggtggtagaa atagctgatt ccatcccgat ggagcaactt
      301 caggatattg atgcgttgat tattgagctt cgggatgtgg gttcgagata ttgtacggct
      361 acccgtactt tgctcaaact cttctatgat cttcataatt cgggcagcga tatctctatt
      421 tatgtgatcg acgggatcaa ttcttccggt cgtcaggtag aggggacaat ggggctaatg
      481 ggcctgccgc acagacatgg actcactttt ggtgagattg ccaacttctt ttattatgag
      541 ctgaacgcaa actttcattt gcacataata tcggctgagg cggagcaggt caataaatat
      601 ctgatgccct ggagcattcc tcccttttcc gattttgcgg gactatttac atcccatttc
      661 tatagtggcc aatgtctttg gatgggtaca aatgtgagtt atggtcacgg tactacgcgt
      721 ccgtttgagc agttcggagc accctttatg gagccacttt atgattttaa tcgtgaaaac
      781 ggatataaaa actggaatga tccggccaat ctgatttacg atgatagtct ctatataaga
      841 tggtgcagat ttgagccggc ctatggtatc tggaaagatg aggtatgctt cggatttcat
      901 ctgatgttta ttcccggaca ctcctatcat gctcttaaac acgctcttcg tttaatacgc
      961 tttgtgcgcg aacagtttcc tgaagatttc agatttgacg gacttgagcc ttttcttcag
     1021 gataatatgc tttttagtta tattgcaggc gaaattgatt gggaattgac cagtgagcac
     1081 ataaagactg aagagcagaa atggcttcgt aaagtgaaaa aatatctgct ttatggtgat
     1141 tccccctggc gtgttaaatg attcaaagat ggagaataaa gttttgaaga tattgtttgt
     1201 ctgccttggc aatatttgcc gcagcccaat ggcagagttt ataatgaaac ataaggtaga
     1261 gcaacttggt gtggccgacc gatttgaaat tgcctctgcc ggtactagcg atgaagagcg
     1321 cggcaatccc gtct
//
LOCUS       DAOSIE010000344         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_90416_length_1304_cov_1.402722, whole genome shotgun sequence.
ACCESSION   DAOSIE010000344 DAOSIE010000000
VERSION     DAOSIE010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90416_length_1304_cov_1.402722"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PLI69_07585"
     CDS             <1..692
                     /locus_tag="PLI69_07585"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOG42384.1"
                     /translation="NTFNESRTSYYLKSIGGYHAAKLRRYQDLIDEHLTQMNMKVINM
                     LNTKYIIVEQDGSPVPMLNKEHLGNAWFVDEIILAETPNQESDALRVIDPATVAVTDS
                     KFADFVTEPTTLTDPSASIELTSYAPDVLTYTSNCAYDKIAVFSEIYYPYGWKAYIDG
                     TPVDHFRVNYLLRALNIPAGKHDIRFEFRPDSVMKGYKVSFAFIGVMYLILISSIVFG
                     FYRVFVTKEDE"
     gene            685..>1304
                     /locus_tag="PLI69_07590"
     CDS             685..>1304
                     /locus_tag="PLI69_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG42385.1"
                     /translation="MNKRFFDRILITGGAGFIGSHIALKLLDKGYSVTVLDNLNSQIH
                     GKDPESTSPLYRSIKDKVQFIHGSVTDKSDWVRALDGQEAIIHLAAETGTGQSMYEIE
                     RYSSVNIGGTALMLDILTNTEHCVKKVIVASSRAIYGEGRYKCDIHGEVFPESRKDED
                     MARGDFECKCPVCGAEVVLMSTTEESKIHPTSVYGISKHTQEQLVMT"
BASE COUNT          382 a          255 c          296 g          371 t
ORIGIN      
        1 ccaatacttt caacgagtcc cgtacttcat attatctaaa gtccataggt ggctatcatg
       61 cagccaaact acgcaggtac caagatctga tagatgagca cctcacacag atgaatatga
      121 aggtgatcaa tatgctcaat acgaaatata tcattgttga gcaggatggg tccccggtac
      181 cgatgctaaa caaggagcat ctgggtaatg cctggtttgt ggatgaaata atcctggctg
      241 aaactcctaa tcaggagagc gatgcgctta gagtcattga tcctgctact gttgcagtaa
      301 ccgacagtaa atttgccgac tttgtcactg aacctacaac acttacagat ccttccgcta
      361 gcatcgaact aacctcctat gcccccgatg tgctgactta caccagcaat tgtgcatacg
      421 ataaaattgc agtattctcg gaaatctatt acccctacgg ttggaaggcc tacattgatg
      481 gcactccggt ggaccacttc cgtgtcaatt atctcctcag agcgctcaat atccctgcag
      541 gtaaacatga tattcgtttt gaattccgcc ccgattcggt gatgaaaggc tataaagtct
      601 catttgcttt tataggtgta atgtatttaa tacttattag ttctattgta ttcggttttt
      661 atcgagtttt tgtaacaaaa gaagatgaat aaaaggtttt ttgacaggat tctaataacc
      721 ggaggagcag gttttattgg ctctcatatt gccttgaaac ttctggacaa gggttattca
      781 gtaactgtac ttgacaattt gaattcgcag attcacggaa aggaccctga aagcacatca
      841 cctctatatc gtagtataaa ggataaagta caatttattc atggatctgt tactgataag
      901 tcggattggg ttcgtgctct ggatggacag gaggctatca tacatcttgc tgcagagacc
      961 ggtaccggtc agagtatgta tgagattgaa aggtatagca gtgttaacat tggcggtacg
     1021 gcgttgatgt tggatattct cactaatacc gaacattgcg taaagaaagt gatagtggcc
     1081 tcttccagag cgatatacgg tgaaggcaga tataaatgtg atatccatgg agaagtattc
     1141 cctgaaagtc ggaaagacga agatatggca agaggagatt ttgagtgcaa atgtccggtt
     1201 tgtggagcag aggttgtgtt gatgtcaact acggaagaga gcaagatcca tcctacttct
     1261 gtatatggaa tatccaagca tactcaagag caacttgtga tgac
//
LOCUS       DAOSIE010000345         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_90665_length_1301_cov_2.914125, whole genome shotgun sequence.
ACCESSION   DAOSIE010000345 DAOSIE010000000
VERSION     DAOSIE010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90665_length_1301_cov_2.914125"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLI69_07595"
     CDS             <1..278
                     /locus_tag="PLI69_07595"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOG42386.1"
                     /translation="WEFLLSIPYGSTMTYGEIAQGIAKQKGVDRLCPRAVGGAVGRNP
                     ISIIIPCHRVVGAGRSLAGYAGGVDRKIALLGLEKVDVGKFSIPKSS"
     gene            429..>1301
                     /locus_tag="PLI69_07600"
     CDS             429..>1301
                     /locus_tag="PLI69_07600"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG42387.1"
                     /translation="MLNFDVKTLFRYIRKKSWKELRAAMQELDHLQLAEAIDRGSEFE
                     SIVLFRFLSRAQAKEVFQELSHEKQEDIVRGLAEQASQVADLLNDMEPDDRTAFLEEL
                     PGEVAQRLMQLLTPDNRAVATTLLGYPEDSIGRLMTPEYVAVRPHFTVEETLAHIRQY
                     GHDSETLNVIYVVDDKWRLLDGLRIREVILASPEQRISDLMDYKFASLNAFDDQETAI
                     SAFKKYDRVVLPVVDTKGILLGIVTVDDILDIAEEEGTEDFQRFGAVQDAIISPLQAT
                     VSFLYRKRVLWLMAL"
BASE COUNT          331 a          268 c          355 g          347 t
ORIGIN      
        1 tatgggagtt tttactctcc atcccttacg gcagcacgat gacctacggt gagatagcgc
       61 agggtattgc caaacaaaaa ggtgttgatc gtctctgtcc gcgggcggtg ggcggtgcgg
      121 tgggtcgcaa ccccatctca atcatcattc cctgccacag ggtggtaggc gcaggtcgct
      181 cactcgcagg ttatgcagga ggagttgaca ggaaaatagc cctactcggg ctcgagaaag
      241 tggatgtggg caaattttcc atccccaaat ctagttgact gattatcttt aatttattat
      301 ctttgcaggc caaaacggat ttttataaaa aatggacgaa gagagttacc aattatttaa
      361 aaaatcttac taaccctttc ctctaaagga accgatctcc ttttgtggaa agctaaaagg
      421 agattattat gctgaatttt gatgttaaaa ctctttttcg gtatatccga aaaaagagtt
      481 ggaaggagtt gagagctgca atgcaggagc tcgaccattt gcagttggca gaggcaattg
      541 atcgcggaag cgaatttgag tcgattgtgc tctttcgctt tctatcgcgc gcacaggcga
      601 aagaggtatt tcaggaactt tcgcacgaaa agcaggaaga tattgtacgg ggattggctg
      661 aacaagcttc acaggtggcc gatctgctca acgatatgga gccggacgac cgtaccgctt
      721 tcctcgagga acttcccggg gaggttgcgc agcggcttat gcagctgctt acccccgaca
      781 acagggcggt agcaaccaca ttgcttggtt atcccgaaga tagcatcggt cgattgatga
      841 cccccgaata tgtggccgtg agaccccatt ttaccgtaga agagaccttg gctcatattc
      901 gccaatatgg gcacgattcc gaaacattga atgtaatcta tgtggtggat gacaaatggc
      961 gattgcttga tgggctccgc attcgggagg tgattctcgc ctctccggag cagcgtataa
     1021 gcgaccttat ggattataaa ttcgcttcgc taaatgcttt tgatgaccag gagacggcga
     1081 taagtgcttt taagaagtat gatagggtgg tgcttccggt ggtggatacc aagggaatat
     1141 tgttgggaat cgtgactgtc gatgatattt tggacattgc tgaagaagaa gggacggagg
     1201 acttccagag gtttggtgca gttcaggatg caattatcag ccctttgcag gctactgtgt
     1261 cgtttttata taggaaaagg gtcctttggc tgatggcttt g
//
LOCUS       DAOSIE010000346         1295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_91258_length_1295_cov_5.173387, whole genome shotgun sequence.
ACCESSION   DAOSIE010000346 DAOSIE010000000
VERSION     DAOSIE010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91258_length_1295_cov_5.173387"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLI69_07605"
     CDS             <1..500
                     /locus_tag="PLI69_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG42388.1"
                     /translation="DSEDHEHVVGEIQARGANIMLGYYKNPEATAAVFTDDGWMRTGD
                     LGIIDNKGNVFIKGRSKNMILSANGQNIYPEEIEDRLNNQPYVVESVVVERDKRIVAL
                     VYPDYDRVSQESMDENDLAKLMEENRIKLNLMLPLYSRIARIELRKNEFEKTPKRSIK
                     RFLYK"
     gene            complement(572..>1295)
                     /locus_tag="PLI69_07610"
     CDS             complement(572..>1295)
                     /locus_tag="PLI69_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOG42389.1"
                     /translation="ALFRFSGGDARKLYNILEIIISTFPEEGEIVIENDVVTDRLQEN
                     IALYDKDGEQHYDIVSAFIKSIRGSDPNAAIYWMARMLAGGEDPLFIARRMLILASED
                     VGLANPNAALLAQSCFDAVHKIGMPESRIILVQTCIYLATSPKSNSAYMAIDEAMALV
                     KSTNEAPPVPLHLRNAPTELMKELGYAKDYKYAHAFEGNFIDQEFLPDSLKGCKFYEP
                     GQNARENEIRKRLQQLWKKYGY"
BASE COUNT          362 a          270 c          316 g          347 t
ORIGIN      
        1 tagattccga agatcacgaa catgtggtgg gagagattca ggcgcgtggt gctaatataa
       61 tgctgggtta ttacaaaaat cccgaggcta ccgctgccgt atttaccgat gatggatgga
      121 tgcgtaccgg tgaccttggc ataattgaca ataaaggcaa tgtcttcatc aaaggacgca
      181 gcaaaaatat gattctaagt gctaacggac agaatatcta tcccgaggag atcgaggacc
      241 gtctcaacaa tcaaccctat gtagtggaat cggttgttgt agaaagggat aaaaggattg
      301 tggccttggt ctaccctgat tatgataggg tatcacagga atcgatggat gaaaatgatc
      361 ttgccaaact aatggaggaa aaccgaatca aactcaacct gatgcttcct ctttatagcc
      421 gcatagccag aattgaactc cgtaagaatg aatttgagaa aacaccaaaa aggtctatca
      481 aaagattctt gtataaataa ttttagttgg gtttcataat ggtggcagag gatttggctt
      541 gttccgaatc ctctgttttt ttgactctcg actaatagcc gtattttttc cagagctgct
      601 gcaaacgttt gcgaatttca ttttcacggg cattttgtcc gggctcataa aatttgcaac
      661 ccttaagaga gtcgggaaga aactcctgat ctatgaaatt gccctcgaaa gcgtgggcat
      721 atttgtaatc ttttgcataa ccaagctctt tcataagttc cgtgggagca ttacgcagat
      781 gaagaggcac cggtggcgct tcattggtgc tttttacaag tgccatagcc tcatctatgg
      841 ccatataggc actattgctc ttgggagagg tggccagata gatacaggtc tgtaccaata
      901 tgatacggct ttcaggcata cctatcttgt gaaccgcatc aaaacaactc tgggcgagta
      961 gagcagcatt gggattggcc aggcccacat cttcagaagc caggatgagc atccttcgtg
     1021 caatgaaaag agggtcttcg ccgcctgcaa gcatacgggc catccagtag atggcggcgt
     1081 tggggtcact cccgcggata cttttgataa atgcggagac gatatcgtag tgttgctccc
     1141 catccttgtc gtataaagcg atattctcct gtaagcggtc tgtaacgaca tcgttctcta
     1201 tgacgatctc accctcttca ggaaaagttg agatgataat ctcgaggata ttatagagtt
     1261 tgcgggcgtc accgccggaa aagcggaaaa gtgct
//
LOCUS       DAOSIE010000347         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_92344_length_1285_cov_4.436585, whole genome shotgun sequence.
ACCESSION   DAOSIE010000347 DAOSIE010000000
VERSION     DAOSIE010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92344_length_1285_cov_4.436585"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PLI69_07615"
     CDS             complement(<1..546)
                     /locus_tag="PLI69_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG42390.1"
                     /translation="MAASDKIIEIRHLSKSLGNKKVLDDISISISKGEFVTFLGPSGC
                     GKTTTLRLIAGFLAPDEGQILFEGKDISDLPPYKRPFNTVFQRYALFPHLDVYDNIAF
                     GLKLKKTPREEINKRVRRVLKMVSMTDYEDRDVDSLSGGQQQRVAIARALINQPKVLL
                     LDEPLAALDLKMRKDMQIELKE"
     gene            complement(550..>1285)
                     /locus_tag="PLI69_07620"
     CDS             complement(550..>1285)
                     /locus_tag="PLI69_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42391.1"
                     /translation="DLTFGNWMISLGKDMILTGGFEYDDWDWDVHPEFATSLWNGLPV
                     YQWGGKVTYTTPNEGHEFAVQATTSPFAERFFTKKLLTASAQYKKYGEVYEVIASASM
                     LGYDPAIPSGAPDFYPVFFLGQRVYLGDWTITLDLSNNNCFASNEDETVSYLRSGVGI
                     NAALTWAPSDKIELTWRGLHSRPLQEFTPPGETRWNTGLICHYYPLKDSDALRIHAYA
                     NYDNIGGDSNLTFSLGIRYNFLINLW"
BASE COUNT          327 a          301 c          304 g          353 t
ORIGIN      
        1 ctccttgagc tcaatctgca tatccttgcg catcttgagg tcgagtgcgg ccagaggctc
       61 gtccagaagg agtactttcg gctgattgat cagcgcacgc gcaatagcaa ccctctgctg
      121 ctgtcctcct gagagggaat ccacatccct atcctcatag tcggtcatgc tcaccatttt
      181 gagcactctg cgtactcttt tgttaatctc ttcgcgagga gtctttttga gtttcaggcc
      241 aaatgcgata ttgtcgtaga catccagatg cgggaaaaga gcataacgct gaaataccgt
      301 attgaatggg cgtttgtatg gagggagatc ggatatatcc tttccctcga agagaatttg
      361 cccctcgtcg ggagccagaa aaccggcaat aagcctcaaa gtagtggttt taccacaacc
      421 tgaaggaccg agaaatgtta caaattctcc tttacttata cttatactta tatcatccag
      481 caccttctta ttacctaagg acttgctcag atgacggatc tcgatgatct tgtctgaagc
      541 tgccatctgt taccaaaggt taataaggaa gttgtatctg atacctaatg agaatgttaa
      601 gtttgaatct ccgccaatgt tgtcataatt ggcgtatgca tggattctga gggcatcact
      661 atccttaaga ggataataat gacagataag accggtattc catctggttt ctcccggagg
      721 agtaaactct tgcaaaggtc tggagtgcag tcctctccac gttaactcaa ttttgtccga
      781 aggagcccag gtaagggcgg cgtttatgcc aaccccggat ctgaggtaac ttacggtttc
      841 atcttcgtta ctggcgaagc agttgttatt tgatagatcc aaagtgatcg tccagtcacc
      901 caggtatact ctttgtccaa gaaagaatac cggatagaag tcgggggctc cggagggtat
      961 agccggatca tatcccagca tggaggcgct ggctatcact tcatagactt ctccatactt
     1021 tttatattgt gcagaagccg tcaggagttt ttttgtaaaa aatctctccg caaagggcga
     1081 agtggttgcc tggacagcaa actcgtggcc ttcattggga gttgtgtagg taacttttcc
     1141 tccccactga tagacgggga gcccgttcca aagggaagtt gcgaactcag gatgcacgtc
     1201 ccagtcccag tcgtcatact caaaacctcc ggtaaggatc atatctttac cgagagagat
     1261 catccaattg ccgaatgtca ggtcg
//
LOCUS       DAOSIE010000348         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_92573_length_1283_cov_4.656352, whole genome shotgun sequence.
ACCESSION   DAOSIE010000348 DAOSIE010000000
VERSION     DAOSIE010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92573_length_1283_cov_4.656352"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..>1283)
                     /locus_tag="PLI69_07625"
     CDS             complement(154..>1283)
                     /locus_tag="PLI69_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42392.1"
                     /translation="TLLFTSCNADASAGLFKQLADAKKPVEIRYRQIIGKDGTDLYFL
                     TNDGIYNYKTDGNTTTTVRENKKGHPVYEAYLDTTNDLIIYLINGGGNAEIHQMKTDG
                     SGDIKLTTTVAEDLDAGWKMKQLLANGLFVIKGTDTVGQTDSFLVAEYQNGPPAEYDK
                     KVLLDDLPGYDRDAVLQMSGRQHWGIGGFNDTTFNAPMILSFVTSGGSYEHYYTDGAF
                     FHQLTSLDKSLAGFWTNGTSLYLITKDGNLYGSVVPAGPGSTAPTPMKDLSRQFPSNA
                     FVYGMSSGGTTHLITKTKTANEALFVVSFADDTITTPVNTKVISTGYASQMSNVNIVS
                     AFEKSAGNLLIATEKNGMFDITITDAATGEGSSAGPEDYTL"
BASE COUNT          325 a          294 c          309 g          355 t
ORIGIN      
        1 ccatagaaca ggtacttgtc catataatcc tcccactgtt atcgatactc attatcaagg
       61 aggaggagag aagagtcaac gcaagatttt ataccacgtt tacatggaac agcctcgcaa
      121 aggcgaggct ggcaacaaag tcggtacatt catttacagg gtgtaatcct caggaccggc
      181 agaggatcct tcacccgtgg ctgcatcggt tatggtgatg tcgaacatcc cgttcttctc
      241 agtggcaatg agaaggtttc ctgccgactt ctcaaaagca ctgacgatgt tgacgttgct
      301 catctgagag gcgtagccgg ttgagatcac tttcgtattg acaggggttg tgatggtatc
      361 gtctgcaaaa ctgaccacaa agagtgcttc gttggctgtt ttggtcttcg tgataaggtg
      421 tgtagttcct cctgatgaca tgccatagac aaacgcattg gagggaaatt gtcttgaaag
      481 atccttcata ggggttggag ctgttgaacc agggcctgct ggaaccacac ttccatacaa
      541 attaccatct ttggttataa gatataggct cgtaccgttg gtccagaatc ctgccagact
      601 tttatcaaga gaggtgagtt gatgaaaaaa tgcgccatca gtgtaatagt gctcatacga
      661 cccaccactg gtgacaaacg agagaatcat gggagcattg aatgtggtgt cgttaaatcc
      721 tccgattccc caatgttgcc taccagacat ctgaagcact gcgtcacgat cgtatccagg
      781 aagatcatcc aacaggactt ttttatcata ctctgcaggc ggaccgtttt gatattcggc
      841 taccagaaag gagtcagttt gaccaacagt atcggtcccc ttaatcacga acaatccatt
      901 tgccaagagc tgtttcatct tccagcccgc atccagatct tctgcaaccg ttgtggtcaa
      961 ttttatatca ccagatccat cagtcttcat ctggtggatc tcggcattgc ctccaccatt
     1021 gatcaagtag atgatcaggt cattggtagt atctagatat gcctcataca ccggatggcc
     1081 tttcttattc tccctgacgg ttgtggtggt atttccatca gtcttgtaat tgtaaattcc
     1141 atcattggtg aggaaataca ggtcagtacc atcttttcca ataatctgcc ggtagcggat
     1201 ttctaccggc ttcttggcat cggcgagctg tttgaagagg ccggctgatg cgtctgcgtt
     1261 gcaggaggtg aagaggagag tga
//
LOCUS       DAOSIE010000349         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_93409_length_1275_cov_4.872131, whole genome shotgun sequence.
ACCESSION   DAOSIE010000349 DAOSIE010000000
VERSION     DAOSIE010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93409_length_1275_cov_4.872131"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLI69_07630"
     CDS             <1..263
                     /locus_tag="PLI69_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42393.1"
                     /translation="DAKTKAIVVMAGIKGKPVSEICNEYRIPQSMYYRWRDQFLGNMH
                     RVFSDNNNRKREEELLRENSRLKKIIGDLTIELKKSEEDWQP"
     gene            260..1036
                     /locus_tag="PLI69_07635"
     CDS             260..1036
                     /locus_tag="PLI69_07635"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOG42394.1"
                     /translation="MKRQESQKTVDRNEPLLVQIERIKADHPFWGYRRVWAYLKYAMG
                     LEVNKKRVLRLMRKHNLLVRPHMALKACRTPQRSKPRPDRPNQWWGIDMTKVKVEDYG
                     WIYIVIVLDWYTKKLVGYYAGLQCKSKHWLEAVDMAVNRQFHDGARGHELFLMSDNGC
                     QPTAISFMKACRTLDIKQVFTSYNNPKGNADTERMIRTLKEECLWLKEWQNPFELLND
                     FKIWAEDFNISYLHSSLGYRTPEQKELEYFNKENTLLSVA"
BASE COUNT          398 a          249 c          351 g          277 t
ORIGIN      
        1 gggatgctaa gaccaaggca atcgtagtga tggcaggtat caagggcaaa ccggtgagtg
       61 agatttgcaa tgagtaccgg ataccgcaaa gtatgtacta tcgttggcgt gaccaattcc
      121 tgggtaatat gcaccgggta ttcagcgaca ataacaacag aaaacgcgag gaagagctgc
      181 tcagagaaaa cagtcggctc aagaaaatca tcggagatct cactatcgag ttaaaaaaaa
      241 gcgaagagga ctggcaaccg tgaagaggca agaatcccaa aaaacggtag atagaaatga
      301 gccgcttctc gtccagattg aaaggatcaa agccgaccat cctttctggg gttaccgtcg
      361 tgtctgggcc tacctgaaat acgccatggg acttgaggta aataagaagc gtgtgctgag
      421 gctgatgcga aagcacaatc tcctggtaag gccgcatatg gcattgaaag cctgccgcac
      481 gccgcagcgg agtaaaccga gaccggacag acccaaccag tggtggggta tagatatgac
      541 caaggtcaaa gtagaagact acgggtggat ttatattgtc attgtcctgg actggtacac
      601 taaaaagctg gtaggatact acgccgggct gcagtgtaaa agtaaacact ggctggaagc
      661 agtggatatg gcggttaacc gccagttcca cgatggagcc agaggacacg aactgtttct
      721 gatgtctgat aacggctgcc agcccaccgc aataagcttt atgaaagctt gccggacgct
      781 ggatatcaag caggtgttta ccagttacaa taacccgaag ggcaatgcag ataccgagag
      841 aatgatacga acgctaaaag aagaatgcct ctggctgaaa gaatggcaga atcccttcga
      901 acttttgaat gatttcaaaa tttgggcgga ggatttcaat atcagctatc tgcattcgag
      961 cttgggttat agaacaccgg agcaaaaaga attggaatac tttaacaagg aaaatactct
     1021 cttatcagtg gcttgactta atggggagca gtacaggagg cggcccggat gtaggaggtg
     1081 gcccggatgt aggaggtggc cctgaggttg ggggcggttg agccgaagtt gaggaaggag
     1141 aaagcgatga ggtagaagtc gttgttgtgg atttagcaca tgataataac aaaattgatg
     1201 taattaatag gcaggctaat gcaaaaaaca ataccgttct tttcaaattt cactcctcct
     1261 tgtaaaaatt ttatt
//
LOCUS       DAOSIE010000350         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_93419_length_1275_cov_3.640164, whole genome shotgun sequence.
ACCESSION   DAOSIE010000350 DAOSIE010000000
VERSION     DAOSIE010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93419_length_1275_cov_3.640164"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLI69_07640"
     CDS             <1..311
                     /locus_tag="PLI69_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOG42395.1"
                     /translation="KSELLSNNKLSLSDILKRADRGIYVTQFNGGNCNAATGDFSYGI
                     EGFLFDNGETVHPVNEMLISGNLVTLWNNLITVADDRLESRKWQIPSLCFSNVNFNGL
                     "
     gene            260..>1275
                     /locus_tag="PLI69_07645"
     CDS             260..>1275
                     /locus_tag="PLI69_07645"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOG42396.1"
                     /translation="MANTFTLLLKRKFQRTLMEKNKKEKKPGLILSLVPVVILVVILA
                     VGVIIFGEDVTGGPSQIALVTAAIVACLIGMLYLKVPWERFADAMGENMSKTSEAIFI
                     LLMIGALTSTWMLSGVVPTMIYYGLKLIHPSIFLLVTFVLCSIISILAGSSWTTVGTI
                     GVAMLNAGQIIGLPTGWLAGAIISGAYLGDKISPLSDTTNLSASVAGVDLYKHVRYNL
                     ITTIPATIICLIIYTLAGFFIPTSSSVDVGAQLTALQETFNISGWLLLIPVVTIYLIF
                     KKVPPILTLFISAFVGALVAFWAQPQIIEQIVTGMGSFKEFFYTILKMMSSEISIETN
                     DPMIT"
BASE COUNT          327 a          295 c          282 g          371 t
ORIGIN      
        1 cgaaaagcga gcttttgtca aataataaat tatctttgtc cgatattttg aagagagcgg
       61 acagaggtat ttatgtcacg cagtttaacg gaggtaactg taatgccgca acaggggact
      121 tctcctatgg catagaggga tttctgtttg acaatgggga aacagtccat ccggtaaatg
      181 aaatgttaat ttccggcaac ctggtcaccc tgtggaataa tctcataacc gttgctgatg
      241 accggctgga gagccgtaaa tggcaaatac cttcactctg cttctcaaac gtaaatttca
      301 acggactctg atggagaaaa ataaaaaaga gaaaaaaccg ggtcttattc tgtcactcgt
      361 tccggtggtg atactggtag tcatactggc tgtaggagta attattttcg gcgaagatgt
      421 taccggcggc ccctcacaaa ttgcgcttgt aactgccgct atagttgctt gtcttatagg
      481 aatgctttac ctcaaagtac cttgggagcg ttttgcggat gcgatgggtg aaaatatgag
      541 caaaacgagt gaagctattt tcatactgct gatgatcgga gcgctgacct ccacctggat
      601 gctgtccggt gtggtgccca cgatgatcta ttatggcctt aaacttattc atccctctat
      661 atttttgctg gtaacatttg tgctatgctc cattatctcc atcctggcgg gcagttcatg
      721 gaccactgta ggcacaatag gagtagccat gctcaatgca gggcaaatta taggcttacc
      781 taccggttgg cttgcaggcg ccattatctc cggtgcatat cttggtgaca agatctcccc
      841 tctctccgac actaccaatc tctccgcttc ggtcgccgga gttgacttat acaaacacgt
      901 tagatataat ctgatcacca ccataccggc cacaatcatc tgtctcatta tttataccct
      961 tgcgggattc ttcataccta cctcctccag tgtggatgtt ggagcccaac ttactgctct
     1021 ccaagagaca tttaatatat ccggatggct cctattgatt cccgtagtga ccatttacct
     1081 gattttcaag aaggttcctc caatacttac actctttatt tcggcttttg taggggcgtt
     1141 ggttgctttt tgggcacagc cccaaataat tgagcagatt gttaccggaa tgggcagttt
     1201 caaggagttc ttctatacca tactcaagat gatgtccagc gagatctcaa tagagaccaa
     1261 cgaccctatg ataac
//
LOCUS       DAOSIE010000351         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_96934_length_1244_cov_2.166526, whole genome shotgun sequence.
ACCESSION   DAOSIE010000351 DAOSIE010000000
VERSION     DAOSIE010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96934_length_1244_cov_2.166526"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            509..928
                     /locus_tag="PLI69_07650"
     CDS             509..928
                     /locus_tag="PLI69_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42397.1"
                     /translation="MARPSNMSHQHLPPGASTGQYVKCVGGRIIWTTETPGGVVTEAG
                     ANAANSISEATGTTIAEKLANATRYAAEGVRGVIAIASTAETNAGLIDDKAITPKKFA
                     NALEAGQIITTTLAVDGGFAATNYGSTVAISGGTANG"
     gene            921..>1244
                     /locus_tag="PLI69_07655"
     CDS             921..>1244
                     /locus_tag="PLI69_07655"
                     /inference="COORDINATES: protein motif:HMM:NF037250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42398.1"
                     /translation="MANRIQLRRDTGANWASVNPVLAQGEVGIETDAVPIQIKVGDGV
                     TAWNSLSYVATGNMDVEGSTLDDLVDGTTYKRIAAALAPAINDETRLPTAAGTTVANN
                     IGAQTG"
BASE COUNT          346 a          254 c          328 g          316 t
ORIGIN      
        1 acttaaatgt ctcggaaatt caattgtccc tcaaattgca gaattgattt tttcacaggc
       61 agtatttgac aaatatagac ttgtacatga aaaataggtt gacaatgtgt cggaatgggt
      121 ttacactagg tgatggagga acagaatggc ggaaactacg gtaatttatc cagacccagt
      181 ccgagggcag gtaaggccgt atggctatgg tacttaccag gttcagacgg gtgccggttc
      241 aaagacgatt gggattcagt ccgacacaaa gtcgattggg gttcaagtcg atgtaatcac
      301 tccaggtagt tactttgtgg aaggtaccgt taatccgacg agtatggtaa tagcggatac
      361 tgcaacatgg tacgaccttt tcggagacgg ggtaactcca cagactacag gaattcagtt
      421 ctctattgac ccagcgaatc tgtctgcaat taggcttcgt gtggtaactg ggaaaatggc
      481 aatctcctat gaggggagga ggacataaat ggcaagacct tcgaatatga gtcatcagca
      541 tcttcctcct ggtgcctcta ccggacaata cgtcaagtgt gtaggcggta gaattatatg
      601 gacaacggaa actcctggtg gcgtcgtcac cgaggccggt gcaaatgcgg ctaatagtat
      661 tagtgaagcc actggaacaa ctattgccga gaagcttgcg aatgctacga ggtatgctgc
      721 tgaaggagta cgaggagtta ttgcgatagc gtcaactgct gagactaatg ctggtcttat
      781 tgacgataag gcaatcaccc ccaagaagtt tgccaatgct ctggaagcag gacagattat
      841 aactacaacc ttggcggttg atggtggctt tgcggccact aactacggtt caacagtggc
      901 tatatcggga ggtacggcaa atggctaata gaattcaatt aagacgtgat acaggagcta
      961 actgggcttc agtaaaccca gttcttgctc agggtgaggt tggcattgaa acggatgctg
     1021 tacctattca aatcaaggtt ggtgatggag taaccgcttg gaactctctc agttatgttg
     1081 ctaccggtaa catggacgtt gaaggtagta ccctcgatga tcttgtagac ggcacaacgt
     1141 ataagcgtat tgctgcagct cttgctcctg ctatcaacga tgaaacccga cttcctactg
     1201 ctgcaggtac aactgtagcc aataatattg gagctcaaac cggt
//
LOCUS       DAOSIE010000352         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_96995_length_1243_cov_4.138047, whole genome shotgun sequence.
ACCESSION   DAOSIE010000352 DAOSIE010000000
VERSION     DAOSIE010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96995_length_1243_cov_4.138047"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLI69_07660"
     CDS             <1..218
                     /locus_tag="PLI69_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOG42399.1"
                     /translation="VLHAEANAITKVAKSGNSSEGATLYVTASPCLECSKLIIQAGIK
                     RVVYHEDYRITDGIDLLRRAGIEVQQL"
     gene            248..>1243
                     /locus_tag="PLI69_07665"
     CDS             248..>1243
                     /locus_tag="PLI69_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG42400.1"
                     /translation="MRLLRISVWIVIIVMLVIMIMRLPKKGVVDLDSTKEDWAKLMLV
                     LNSVDKDYVDDIDHKAVTEKLLPAVMAELDPHSVYLPPVDLEKADEALQGGFDGIGIQ
                     FNVPNDTAIVTNVISGGPSEKAGLLSGDRIVKIDGKNIAGVGMKQDSMVSMMRGPRGT
                     KVKLELKREGEGALIPFTIVRDMIPVNSLDVAFMINDTTGYIKLSKFSKTSYFEFLQA
                     TLTLREKGMKRLIFDLRDNTGGYFDQAMMLSNEFLEKGDIVVYMEGKSRPRQDFIADG
                     KGSCKDIELAVLINEASASSSEIFAGAMQDNDRALIYGLRSFGKGLVQEPIYFSDG"
BASE COUNT          355 a          236 c          331 g          321 t
ORIGIN      
        1 atgtcctaca tgccgaggca aatgctataa caaaagttgc caagagcggt aacagcagcg
       61 aaggggcgac actctatgtg acggcttctc cctgtctgga atgctcaaaa ctcataatac
      121 aggcgggcat caaaagagtg gtctatcacg aagattaccg tatcactgat ggtatcgatc
      181 tgctccgcag ggccggcatt gaggttcaac agctgtaatc ccctttatca tgagtagtga
      241 caaaactatg agactactga ggatatcggt atggattgta atcattgtga tgttggtcat
      301 aatgattatg aggcttccta aaaagggtgt ggtagatctg gattcaacaa aggaagattg
      361 ggctaagctg atgcttgtgc taaatagcgt ggacaaagat tatgtagacg atatagacca
      421 taaggctgta acggagaaac tccttcctgc agtcatggct gaacttgacc cccattcggt
      481 atatctgcct cctgtagatc tggaaaaagc cgatgaggca cttcaaggag ggtttgatgg
      541 tataggtatc cagtttaatg tccccaatga tacggcaata gttaccaatg tcatttccgg
      601 tgggccttcc gaaaaggcgg gattactcag cggagataga atcgtcaaga ttgacggcaa
      661 aaatattgcc ggagtgggta tgaagcagga ctcaatggtc tctatgatga ggggaccaag
      721 gggtacaaag gtaaaactgg agctcaagag ggagggagaa ggagctctta taccatttac
      781 cattgtcagg gatatgatcc cggttaacag cctggatgtc gcatttatga tcaacgatac
      841 caccggatac attaaactct ccaaattttc caagacaagt tatttcgaat tcctccaggc
      901 tacgctaact cttcgtgaga aggggatgaa gcgacttatt ttcgatttga gggataatac
      961 gggcgggtat tttgatcagg cgatgatgct ttccaacgaa tttctggaga agggagacat
     1021 tgtggtctat atggagggta aaagtcgtcc gcgccaggat tttatagcgg atggtaaagg
     1081 cagttgtaag gatatagagc tggcggtcct aattaatgaa gcttccgcat cttccagtga
     1141 gatttttgca ggagcaatgc aagacaacga cagagcactt atttatggct tgcgctcatt
     1201 tggaaagggt cttgtgcagg agccgatcta tttttcggat gga
//
LOCUS       DAOSIE010000353         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_97307_length_1241_cov_1.852445, whole genome shotgun sequence.
ACCESSION   DAOSIE010000353 DAOSIE010000000
VERSION     DAOSIE010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97307_length_1241_cov_1.852445"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..>1241)
                     /locus_tag="PLI69_07670"
     CDS             complement(95..>1241)
                     /locus_tag="PLI69_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG42401.1"
                     /translation="VTANIISLSTYYLPIIEGIFDMYDLPKELKAMAMIESAFNPKAV
                     SRARARGMWQFMHYTARLYDLDMTSFVDERYDPYKSCHAAARYLRDSYNIFGDWSLAI
                     ASYNCGAGNVLKAIRRSGGKTDFWDIYNYLPRETRGYVPIFFAALYTLKYYPEHGIVP
                     QQSSLPAHVDTIHVNRMLHFQQISDVIGIPMEELRQINPQYLHDIIPGTEKTYILRLP
                     HNYTMQFVNNEESIYSYKDTLFFSDVAIKKVKERGGTEGSMTTHTVRSGETLGGIARK
                     YRTTVSNIKRWNNLRSDMIRVGQKLRIGGSAASSKSSSSTKASGSTTGTATKDGYITY
                     TVKKNDTLYGIASQFSGVTLNSLLKLNGFTQSTKIYPGMVIKIKKAQ"
BASE COUNT          296 a          260 c          315 g          370 t
ORIGIN      
        1 tggagaatta ctacggcaag ggttggagct ggtatttcct tatgagatgg tcgccagcga
       61 agtttgcaga tttatggttc aaatattcga agaactactg tgccttcttt atcttgatta
      121 ccattcccgg atagatcttg gtggattggg taaatccgtt cagcttgagc agtgagttga
      181 gggttacacc cgagaattgg ctcgcaatgc cgtaaagcgt gtcatttttc ttgacggtat
      241 aggtgatata tccatcttta gtagcagttc cggtagtgct gccggaggct ttcgttgacg
      301 aactcgattt tgaggatgca gcgctgccgc ctatgcggag tttctggcca acacggatca
      361 tgtcgcttct aagattattc catctcttga tgttggagac tgtggtgcga tatttccgtg
      421 ctatgccgcc caatgtttcg ccggatctta cggtatgggt ggtcatactt ccctcggtac
      481 cacctctctc cttaaccttt ttgatagcca cgtcgctgaa gaagagtgta tccttgtaag
      541 aataaatcga ctcttcattg tttacgaatt gcatcgtgta attgtgcgga agtctgagga
      601 tataggtctt ttccgttccg ggaatgatat cgtggagata ctggggatta atctggcgga
      661 gttcttccat cggtatgccg ataacatcgg atatttgctg aaagtgcagc atcctgttga
      721 catgaattgt gtccacatga gcgggaagtg aagattgttg gggtactatg ccgtgctccg
      781 ggtagtattt caaagtatag agtgcggcaa agaaaatggg aacataaccg cgcgtctctc
      841 ttggaagata attgtaaata tcccaaaagt cggtctttcc gcctgaacga cgtattgcct
      901 ttagcacatt gccggcgcca cagttatagg aggctattgc aagagaccaa tccccgaaaa
      961 tattgtatga atccctgaga tacctcgctg ccgcatggca ggatttatat ggatcgtatc
     1021 tttcatccac aaatgaggtc atatccaggt cataaagtct tgcggtgtag tgcatgaatt
     1081 gccacattcc tctcgctctg gccctcgaaa cggctttggg attgaatgca gactctatca
     1141 tagccatggc cttgagctcc ttgggaaggt cgtacatatc gaatattccc tctattatcg
     1201 gcagataata ggtagaaaga gatatgatgt tggcggttac c
//
LOCUS       DAOSIE010000354         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_98562_length_1230_cov_2.761702, whole genome shotgun sequence.
ACCESSION   DAOSIE010000354 DAOSIE010000000
VERSION     DAOSIE010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98562_length_1230_cov_2.761702"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="PLI69_07675"
     CDS             <1..1177
                     /locus_tag="PLI69_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOG42402.1"
                     /translation="ADQLIDIGPLAGKLGGEVVYQGPINDSDTSSITLRHLSERVLMK
                     IPSTGRRWNHYIEVVGAMQNNLKNINVRFPLKCLTAVTGVSGSGKSSLVGDILYPALY
                     RHIHQLGSKPGLFRGLRGDLGRINAVEYVDQNPIGKSTRSNPVTYLKVYDDIRKLFSE
                     QPYAKMNNYGHSHFSFNIDGGRCPECQGEGVIKIEMQFMADVAITCEACGGKRFQPDI
                     LEVRFQGKNISDILSMSVDEAIEFFSAQEDPTAKRIAEKLQPLSDVGLSYVSLGQSSS
                     TLSGGESQRIKLASFLGKESSSGSIMFIFDEPTTGLHFQDIEKLLQSFDALIEKGHSV
                     VVVEHNLDIIRRADWIIDLGPDAGDKGGELVFEGTPEMLALHPELPTGAALAGEFRN"
BASE COUNT          351 a          260 c          305 g          314 t
ORIGIN      
        1 tgctgaccaa ctgattgaca ttggaccgct ggccgggaaa cttggtggtg aggttgttta
       61 tcaaggccct ataaacgaca gtgatacttc gtcaataacc ctcagacacc tatcggaaag
      121 ggttctgatg aagatacctt ccaccggtag aagatggaat cattacatag aggtggtggg
      181 tgcaatgcaa aacaatctca aaaatatcaa tgtccgcttc cctctcaaat gtctaaccgc
      241 agttaccggt gtaagtggca gtggcaagtc ttctcttgtg ggagatatac tttatccggc
      301 actataccgc catattcacc agttgggtag caaaccggga cttttccgtg gactaagggg
      361 agatctgggt agaataaatg ccgtagagta tgtagaccag aatcccatag gcaagagcac
      421 caggtccaat cctgtaacct acctcaaggt ctatgacgac attcgcaagc ttttctccga
      481 gcagccctat gctaaaatga acaactacgg acactcccat ttttcattca atattgatgg
      541 aggacgctgt ccggaatgtc agggtgaggg tgttatcaaa atagaaatgc agtttatggc
      601 tgacgtagcc atcacttgcg aggcttgcgg tggtaaaagg ttccaaccgg atattctgga
      661 agtgaggttt cagggcaaaa atatcagcga cattctcagt atgagcgttg atgaggcgat
      721 agagtttttc tccgctcaag aggaccctac ggcaaaaagg attgcagaga agctacagcc
      781 tctcagtgat gtcggactct cctatgtaag tctggggcaa agcagcagta cactcagcgg
      841 aggagagagt cagagaatta aacttgcctc tttcctcgga aaggagtcct cttccgggag
      901 cattatgttc atatttgacg aaccaaccac cggacttcat tttcaggata ttgaaaaact
      961 gctgcaatca tttgatgctc ttatagaaaa aggacactca gtggtggtgg tagagcacaa
     1021 tctcgatata ataagaagag cggactggat tattgatctt ggtcccgatg ccggagacaa
     1081 aggtggagaa cttgtatttg aaggaactcc tgaaatgttg gcgcttcacc ctgaacttcc
     1141 gactggtgca gctttggcag gagagttcag gaattagttt ccacaatcga gactctttga
     1201 ttatatttgt actttcaaaa agatgaagac
//
LOCUS       DAOSIE010000355         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_98870_length_1228_cov_1.780904, whole genome shotgun sequence.
ACCESSION   DAOSIE010000355 DAOSIE010000000
VERSION     DAOSIE010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98870_length_1228_cov_1.780904"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PLI69_07680"
     CDS             <1..1073
                     /locus_tag="PLI69_07680"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG42403.1"
                     /translation="ELNQIVSEFYQDVKSANIPVFQYCIFEKDISIGGVILADSTYNY
                     TQPANDSSIFQAASLSKPLFAYIVMRIVDKGEIDLDTPIYRYTDIERFEDKEMAAILT
                     PRMVLSHTTGLPNWSAKSSSDEWPTSVIKFKYPADSCFSYSGEGFAFLQRAIEAIRGA
                     SLDVIAREEVFVPLGMNSTSYGWLPEYDSIALDGFNRVAKNRGQGRHPRENSAYTLRT
                     NASEYMRFLIAVMEGRGLSAASHAEMLTPQSHAIRYADHPDCDSTIRWTIGLGAIYSD
                     TTAAEKGEAPRYYYHLGDNGSFKALFMFRPEDRRGVVYFTNSANGHEIITSVTRATFG
                     ELLSIQDWLYKSKGKGRRSVSN"
BASE COUNT          338 a          275 c          284 g          331 t
ORIGIN      
        1 aagagctgaa tcagatagta tctgagtttt accaagatgt caaatccgca aacattccgg
       61 tttttcaata ttgcattttt gaaaaagaca tttcgatcgg aggagttatt cttgcggaca
      121 gcacgtataa ctacacccag cctgccaatg attcaagtat cttccaagca gcctccctta
      181 gcaagccgct atttgcatat attgtgatga gaattgtgga taagggagag attgatctcg
      241 acacgccaat ataccgatat accgatatag agcggtttga ggataaagag atggccgcta
      301 ttcttactcc aaggatggtc ctctcacata ccaccggatt gcctaattgg tcggcgaagt
      361 cctcatctga tgaatggccg acgagtgtga taaaatttaa atatccggca gattcctgtt
      421 tctcctattc tggtgagggc tttgcattcc tgcagcgtgc tatagaagca atccgtggtg
      481 catcactcga tgtgattgca cgcgaagagg tttttgttcc gctgggaatg aattctacct
      541 cttacggctg gctgccggag tacgacagca ttgcgcttga cggttttaat cgtgttgcaa
      601 aaaatcgcgg acagggacgt caccctcgtg agaatagtgc ctatactctg cgtacaaatg
      661 cttctgaata tatgcgcttt ttgatagccg ttatggaagg aagaggtctt agtgcagcat
      721 cacacgctga aatgctcacg ccacagtcac atgcaattcg ttatgcggat catcctgatt
      781 gcgactctac aatccggtgg accatcggtt taggcgccat atattctgac acgactgccg
      841 ctgaaaaagg ggaggcccct cgctactatt atcatttggg tgataacggt agcttcaagg
      901 cactctttat gttccgtccg gaagatcgtc ggggagtagt ctattttacc aacagcgcca
      961 acggtcacga aataattact tcggttacgc gcgccacctt cggagaattg ctttctatac
     1021 aggactggct ttacaagtca aaaggaaaag gaaggagatc cgtaagcaat tgaagatatt
     1081 ttagtctgtc atactcccaa ctcgaaacta accaaatatc attatcacca gaatagttac
     1141 cggagcaaca aaacgaatga taaagaatgt ggtctccgta aaccacctgg gaagtttgag
     1201 agtaccacta ttggtgagtt cgtccatc
//
LOCUS       DAOSIE010000356         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_99291_length_1224_cov_2.728828, whole genome shotgun sequence.
ACCESSION   DAOSIE010000356 DAOSIE010000000
VERSION     DAOSIE010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99291_length_1224_cov_2.728828"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLI69_07685"
     CDS             <1..699
                     /locus_tag="PLI69_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG42404.1"
                     /translation="YIEKHSISRLIGSPPGYVGYDQGGQLTEQVRLKPYSVVLLDEVE
                     KAHPDIYNILLQVFDEGRLTDSSGRTVDFRNTILILTSNIGSRKLRDFGGDVGFSTSS
                     TDRDFRRKSIIDKALERQFSPELLNRLDDRIYFNSLSREDIFSIIEIEFEDLADRVGH
                     LGYELEVDQSARTFVADEGFDPELGARPLKRAIQRHIEDPLSELIISDGSGSGVLKVT
                     FDEQSGGIAVTHIA"
     gene            complement(683..>1224)
                     /locus_tag="PLI69_07690"
     CDS             complement(683..>1224)
                     /locus_tag="PLI69_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42405.1"
                     /translation="VFARKCEVKPLRAQQAADFLEKYHSYGTSRCRYRYGLLYEETLV
                     AVATFSAGRPMKRLVGGEERIFESYEWVRFASLPDCRISGGMGRLLEAFVSDVSPDEV
                     MSYADYEWSPGDAYIRLGFEKVGEKPPMRFYVERTTYRRISEKKVSADKRFRGETFPK
                     EKYALISNLGSAKYLKQYV"
BASE COUNT          321 a          316 c          278 g          309 t
ORIGIN      
        1 tacatcgaaa aacactctat aagtcgtctt ataggctccc ctccgggcta tgtgggctac
       61 gaccagggtg gccagctgac agagcaggtt cgtctcaaac cctatagcgt tgtactcttg
      121 gatgaggtag aaaaggccca cccggacata tacaacatcc tactgcaagt atttgacgaa
      181 ggaaggctta ccgatagcag tggccgtacg gtggacttcc gcaacaccat cctgatcctc
      241 acttccaata tcggaagccg taaacttcgg gacttcggag gcgatgtggg attttccact
      301 agcagtaccg acagggactt ccggcgcaag tccattatag acaaagctct ggagcgacaa
      361 ttctctcctg aattgctcaa tcgtctggac gacagaattt acttcaactc cctctcaagg
      421 gaggacattt tcagcataat tgagatagaa tttgaggacc tcgccgaccg agttggtcac
      481 cttggatacg aacttgaggt agatcaaagc gcccgtacat tcgtagccga tgagggattt
      541 gaccccgaac ttggcgcaag acctttaaag agggctattc aaaggcatat cgaagacccg
      601 ctgtccgaac ttatcataag cgacggatcg ggcagtggag ttttgaaagt tacttttgac
      661 gaacaatccg gcgggattgc agtcacacat attgcttgag atatttagcg ctgcccagat
      721 tggatatcag cgcatatttt tccttaggga aagtctctcc gcggaatctc ttgtcagcgg
      781 aaactttttt ttccgaaatt ctcctgtatg tagtgcgttc tacgtaaaaa cgcatcggag
      841 gtttctcccc caccttttca aaaccaagtc gaatataggc atctccggga gaccattcgt
      901 aatccgcgta tgacattact tcgtcgggac tgacatcact cacaaatgct tcaaggagtc
      961 gtcccatacc tccgctaata cgacagtccg gcaaagaggc aaatctcacc cattcataac
     1021 tctcaaagat gcgttcctct cctccgacaa gacgcttcat aggcctgcct gcggaaaatg
     1081 tggctactgc gaccagcgtt tcctcataaa gaagcccgta acgatacctg catcttgagg
     1141 ttccataact atgatatttt tcaagaaaat cggctgcttg ttgcgctctc agcggtttta
     1201 cttcacattt cctggcgaaa acgg
//
LOCUS       DAOSIE010000357         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_100102_length_1217_cov_3.379518, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000357 DAOSIE010000000
VERSION     DAOSIE010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100102_length_1217_cov_3.379518"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..>1217
                     /locus_tag="PLI69_07695"
     CDS             219..>1217
                     /locus_tag="PLI69_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOG42406.1"
                     /translation="MNNIIELQRNYFNTGATLGYDFRLNQLKRLKSIIRKYEPEILEA
                     FLKDYNKSAFDVLSTEIGYSLIELNYLIKHLKKLMKPKKAKTTTINRPSKGYIVPEPY
                     GVTLVVAPWNYPFHLSIVPLAAAMAAGNTVVLKLSTNTPTLSGLLYTIIMQHFPREYI
                     YVTWNSPTERETLFGGRFDYIFYTGSPAVARELQLRQAQDGRLTPMTLELGGKSPCIV
                     DKDADIDAAARRIVWGKYLNAGQTCVAPDHVYVERSVKDELVSKMIAYIKEFYYNKNG
                     GEYALRDDYTYIVNEKAVERLRRYLEDVRVLWGGKAEGRRMEPTIIDMVTEKSPIMQ"
BASE COUNT          346 a          249 c          286 g          336 t
ORIGIN      
        1 agtaccgttt ctaatcgatc catagtttgt ggccggatgg cttgatgacg atgagttatc
       61 gggtcatccg gctccatttt agccatctct caatgtgtga ggtcagtcag tttgtcatta
      121 tgtcgggata gcatattcgg tggcatgata ttgggaatgc cctatacgga tttgctccaa
      181 acgcacctct tttcttcaaa aatgattaaa tttgtattat gaataatatt atagaattac
      241 aacgcaacta tttcaacaca ggagccactt taggctatga tttccgacta aaccagctta
      301 aaagactcaa aagcatcatt cgtaagtatg agcctgagat tctggaggca tttctaaagg
      361 attacaataa atctgcattt gatgtactct ctacggagat aggatattcg ctaattgagc
      421 tgaattatct tataaagcat cttaaaaaat tgatgaagcc taagaaggct aaaaccacta
      481 cgattaaccg tccctcaaaa ggctatattg tacctgagcc ctatggagtg accctcgtag
      541 tagctccatg gaattatccg tttcatctct ccatagttcc tttggcggct gccatggcag
      601 cgggtaatac agtagtgctg aaactctcca ccaacactcc gactctctca ggacttcttt
      661 ataccataat tatgcagcac tttccgagag agtatatcta tgttacctgg aattccccta
      721 ctgagaggga aactcttttc ggaggcagat ttgactatat attctatacg ggatctccgg
      781 cagtcgcccg cgaattgcaa ttgaggcagg cacaggatgg tcgcctgact ccgatgacgc
      841 tcgaacttgg aggcaagtct ccctgtatag tggataagga tgcggatatt gatgcagctg
      901 cacgcagaat tgtatggggt aaatatctca acgcgggaca aacctgtgtg gctcccgatc
      961 acgtttatgt ggagcggagc gtcaaagatg aactcgtttc gaagatgatt gcatatatca
     1021 aggagtttta ttacaacaag aatggagggg aatacgctct gcgagatgat tatacataca
     1081 tagtgaatga aaaggcggtt gagcgtctgc ggcggtatct ggaggatgta agagtacttt
     1141 ggggaggcaa ggcagaaggt cgcaggatgg aacccacaat catcgatatg gttactgaga
     1201 agagtcccat aatgcaa
//
LOCUS       DAOSIE010000358         1205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_101558_length_1205_cov_3.717391, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000358 DAOSIE010000000
VERSION     DAOSIE010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101558_length_1205_cov_3.717391"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /gene="tilS"
                     /locus_tag="PLI69_07700"
     CDS             <1..385
                     /gene="tilS"
                     /locus_tag="PLI69_07700"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOG42407.1"
                     /translation="LKVYPGNQDYDDLKVSIKVFTKPAGFNPKKAPEGTLYVDGRRLK
                     FPLKCRRWKPADRFRPFGMKGFKKLSDFFIDLKLDVEEKKRQIVVTTLDKKGVEQIVC
                     IVGKRIDDRWKVTAATKQIVSITKV"
     gene            complement(401..>1205)
                     /gene="sppA"
                     /locus_tag="PLI69_07705"
     CDS             complement(401..>1205)
                     /gene="sppA"
                     /locus_tag="PLI69_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOG42408.1"
                     /translation="GSDRSISAVKFSQTLEKIRKDSTIKAVVLRVNSPGGGVTASEMI
                     HREISLLKEVKPVIASYGDFAASGGYWISAKADKIFANKSTLTGSIGVFALIPNLNKT
                     MEKVLHIHPVAITTNKHGDALSGMRALASTEKEYLQHMVDKTYTNFTTLVATSRNMSV
                     EQVDMIAQGRVWAGGDALEIGLADMEGGLVDAISYAAEMAGLSKYRLVEYPQVKTTLE
                     KFMESLSKTGADIATIADPFAYIERCYSRLKEATGVIHYARLPIEYDIR"
BASE COUNT          319 a          261 c          280 g          345 t
ORIGIN      
        1 tcttaaagtt tatcccggaa atcaggatta tgatgatctc aaggtctcaa taaaagtctt
       61 caccaaaccg gcaggattta atccaaaaaa ggctccagaa ggtactctct atgtggatgg
      121 cagacgcttg aaatttcctc tgaaatgtcg aagatggaag cctgcagatc gtttcagacc
      181 ttttggtatg aagggtttca aaaagttgag tgactttttt atagacctta agctggatgt
      241 agaggaaaag aagcgtcaga tagttgtcac tactctcgat aaaaaaggag tggagcagat
      301 tgtctgcatt gtgggtaaaa gaattgatga ccgctggaaa gtcacagcgg ccaccaaaca
      361 gattgtttcg ataacgaaag tctaatctct attatttcct ctatctgata tcgtattcta
      421 taggaagacg ggcatagtga ataacgcctg tggcctcttt cagacgggaa taacaccttt
      481 ctatatatgc gaaggggtct gctattgtag caatatcggc acctgtctta gagagggatt
      541 ccatgaattt ttccagggta gttttaacct gagggtactc cacaagacga tactttgaga
      601 gaccggccat ttcggctgcg taggagatgg catcaaccaa tcctccttcc atatcggcaa
      661 gcccaatctc aagtgcgtcg ccaccggccc atactctacc ctgagcaatc atatccacct
      721 gttctacact catattcctg cttgttgcca ccagggtggt aaagttggta tatgttttat
      781 ctaccatatg ctgcaaatac tccttttcag tagaggcgag ggcacgcata ccactcaatg
      841 catcaccgtg tttgttggta gttatggcta ccggatggat gtgcaggact ttttccatcg
      901 tcttgttgag gttgggtata agcgcgaata ctccgatgga gccggtaagg gtggatttgt
      961 tggcaaatat tttgtcggct ttggctgaaa tccaataacc gcccgaggct gcaaagtctc
     1021 catagcttgc aatcaccggc ttaacctctt tcagcaggga tatttcacgg tggatcattt
     1081 ctgaggccgt cacaccgcct ccgggagagt ttactcgcaa taccacagct ttaatggtgg
     1141 agtcttttct aatcttttca agggtttgag agaatttgac agctgaaata gatctgtcac
     1201 ttcct
//
LOCUS       DAOSIE010000359         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_101824_length_1203_cov_3.426829, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000359 DAOSIE010000000
VERSION     DAOSIE010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101824_length_1203_cov_3.426829"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..696
                     /locus_tag="PLI69_07710"
     CDS             151..696
                     /locus_tag="PLI69_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42409.1"
                     /translation="MKTRLIFAMALIMMGTISCEKIGVGSWDVPKNTYQDLVFPKEAT
                     LTFISEKEGKLNLLLTFGVEIDFDKMGDHCYSNVSFNLRGVPYEVNGKRLRICADKLH
                     GSMSYTESYYGGCKSNHEAFSGTYSGDIKLTRTSYTNSSAFSPTDRLSGKWTIRFVSD
                     SGEKLLFEVVDPNLRFVKTGI"
     gene            775..>1203
                     /locus_tag="PLI69_07715"
     CDS             775..>1203
                     /locus_tag="PLI69_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42410.1"
                     /translation="MKKNIKLLHAEERPREKLMSKGASALTCAELIAILLRSGHANLN
                     AIDLSREILLKAGNSLNILSTFTAHQLMRINGVGQAKALSILAAFELGRRMAVEIPEP
                     MPQIHSSIQVNGIMSPIISNLPHEECWVLYLNRSNRLIGKE"
BASE COUNT          366 a          247 c          250 g          340 t
ORIGIN      
        1 aaaacgcatt gcaactatgt ggatctgaga tgtttgatgg catttttttt gcttattatt
       61 attattatta ttattattat tgcaaagtac taacaacctt tatctcacaa acatcacaat
      121 taaattaatt acttttaaac aattaatgcc atgaaaactc gattgatatt tgcaatggct
      181 cttattatga tgggaaccat ttcttgtgag aaaataggtg tcggaagttg ggatgtgccg
      241 aagaatacct accaagattt agtatttcca aaggaagcca ctcttacttt tatttctgaa
      301 aaagagggta agttgaatct gttattgact ttcggggtcg aaatcgattt tgataaaatg
      361 ggagatcatt gctattccaa tgtaagcttt aatctaagag gcgtacctta tgaggtcaat
      421 gggaaaagac tgcgcatttg tgccgataaa ctacacggca gtatgagtta caccgaatct
      481 tattacggag gttgtaagtc caatcatgag gcattttccg gcacctattc aggtgacatt
      541 aagctcacca ggacttctta caccaacagt tcagctttct ctcccaccga ccggctgagc
      601 ggaaaatgga ctatccgctt tgtaagcgat agcggagaaa aactcctgtt tgaagtggtg
      661 gaccccaacc tgagatttgt aaaaaccggt atataaattg tctttttgac atatgacata
      721 gagaaaactt tccaatcttt ggaaagtttt ttttatatcg ttaaggaaca atttatgaaa
      781 aagaacatca aattattgca tgcagaagag agaccacgtg agaaattgat gtccaaaggg
      841 gcttctgccc tcacctgtgc ggagctgatc gcaatcctgc tgagatccgg ccacgcaaac
      901 ctcaacgcaa tcgatctctc gagagagatt ctcctgaagg cgggaaacag cctcaacata
      961 ctctcgactt ttacggcgca tcaactgatg agaatcaacg gagtaggtca ggccaaggct
     1021 ctctccatcc tggcagcatt cgaactcggg agaagaatgg ccgttgaaat tcccgagccg
     1081 atgccacaga ttcatagctc aattcaggta aacggcataa tgtcgccaat tatcagcaat
     1141 cttcctcacg aagagtgttg ggtgctatac ctcaaccgtt ccaaccgtct cataggcaag
     1201 gaa
//
LOCUS       DAOSIE010000360         1193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_103116_length_1193_cov_2.623023, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000360 DAOSIE010000000
VERSION     DAOSIE010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103116_length_1193_cov_2.623023"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PLI69_07720"
     CDS             complement(<1..937)
                     /locus_tag="PLI69_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOG42411.1"
                     /translation="MRLLSILSALLLFAGSLFAQPFTQTKGTLTPAQEHFSDMRFGVF
                     IHWGIYSMLGQGEWVMQNQDIHWQEYEKIAGAFYPIHFDAKQWVSAIKEAGARYICIT
                     SRHHDGFSMWNTAQSEYNIVDATPFGRDVLKELAQECEQQGIQLHFYYSTLDWRREDY
                     PRGRTGRGCGRGPQQPIDNYFNFMYAQLTELLTQYGKIGCIWLDGHWDHEEDPVEFDW
                     RYDTLYPLIKSLQNDCLIGNNHHLDPFEGEDIQIFERDVPGENKIGLMQGGVSENLPL
                     ETCQTMNNSWGYRIKDQNYTSAPELIRYLAYTAGRN"
     gene            complement(989..>1193)
                     /locus_tag="PLI69_07725"
     CDS             complement(989..>1193)
                     /locus_tag="PLI69_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HOG42412.1"
                     /translation="IIGMDSSNISDMNYAFGGDPEGKIRLLLDYTGHSRNIADPWYTG
                     DFQATWDDVNEGCDALLADLGFI"
BASE COUNT          331 a          300 c          248 g          314 t
ORIGIN      
        1 catttcgacc cgcagtatag gccaaataac ggataagttc gggagcactc gtgtagtttt
       61 gatctttgat acggtacccc caggagttat tcatagtctg gcatgtctcc agagggagat
      121 tttcacttac tcctccctgc attaatccga ttttattttc acccggtaca tccctttcga
      181 atatctgaat atcctcaccc tcaaaggggt caagatggtg attatttcca atgagacaat
      241 cgttctgaag cgatttaatc agcggatata gggtgtcata acgccaatca aactccacgg
      301 gatcctcctc atgatcccaa tgaccatcca gccaaataca tccgatctta ccatattggg
      361 tcagcaactc cgtcaactgt gcgtacataa agttgaaata gttatctatg ggctgctgcg
      421 ggcctctccc acatccgcgt cctgtacgtc cgcgaggata gtcctctcta cgccaatcca
      481 gggtggagta gtaaaagtga agctgaatac cctgttgttc gcactcctga gccaactcct
      541 tgagcacatc acggccaaaa ggagtagcat ccacaatatt gtactccgac tgagcggtat
      601 tccacatcga gaaaccatcg tgatggcgcg aagtaatgca gatatagcgc gctcccgcct
      661 ctttgattgc tgagacccat tgtttggcat caaaatgtat gggatagaaa gcaccggcta
      721 ttttttcata ttcctgccag tggatatcct ggttttgcat cacccactcg ccctgcccaa
      781 gcatactgta gatgccccaa tggataaata caccgaatct catgtccgag aaatgctcct
      841 gagcaggagt cagggtaccc ttagtttgtg taaaaggttg tgcaaaaagg ctcccggcaa
      901 agagtagcag agccgatagg atagataata gtctcatagt tcacaaattt agaggtgaat
      961 ataacacttt ttagggattt tggaaaaatc aaatgaaccc taaatcagct aaaagggcat
     1021 cacaaccttc gttaacatca tcccatgtcg cctggaaatc accggtatac cagggatccg
     1081 ctatatttcg tgaatgtccc gtataatcga gcaacagccg gattttccct tcgggatctc
     1141 cgccaaaggc ataattcata tcactaatat ttgatgaatc catcccgata atc
//
LOCUS       DAOSIE010000361         1178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_105067_length_1178_cov_4.269813, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000361 DAOSIE010000000
VERSION     DAOSIE010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105067_length_1178_cov_4.269813"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="PLI69_07730"
     CDS             <1..176
                     /locus_tag="PLI69_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOG42413.1"
                     /translation="PNEEAYNGVVGAFEGGGYSAKGIYRPYFDCRMKSNSAEGFCPVC
                     NAAIQRMIDYYCK"
     gene            180..899
                     /locus_tag="PLI69_07735"
     CDS             180..899
                     /locus_tag="PLI69_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOG42414.1"
                     /translation="MIIERYDYFLIDLDRTLWDFDANTDAAMDMLLDKWNPPIGDREL
                     FLERYEVINRRLWDEYEARVIPKEFLRRERFHQTLREHYGIDDADAAEQMGRDYLDFM
                     ALGDILMPGAREVLEAIAARGGKMAIVSNGFKEVQYRKLRISKIDHFFSAVMISEEVG
                     VHKPDSLIFQKALEALCGEGVEIDEKVKARTIMIGDDYPNDIVGAINFGIDSFYYNPS
                     NKENPGNAVWHSRDLLSLLSD"
     gene            complement(896..>1178)
                     /locus_tag="PLI69_07740"
     CDS             complement(896..>1178)
                     /locus_tag="PLI69_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOG42415.1"
                     /translation="TAEVREIFKISKVGTIAGCMVKEGKLTRTSKVRVIRDGIVIYTG
                     KLGSLKRFKDDVKEVASGFDCGLNIDGFNDVKIGDIIEAYTIVEIKRKL"
BASE COUNT          327 a          246 c          289 g          316 t
ORIGIN      
        1 cacctaacga agaggcttat aatggagttg ttggggcatt cgaagggggt ggatactctg
       61 caaaagggat ttacagaccc tatttcgatt gccgtatgaa aagcaattca gccgagggtt
      121 tctgcccggt atgcaatgct gcaattcaaa gaatgattga ctattactgt aagtagccga
      181 tgataattga gagatacgac tattttctta tagaccttga caggactctc tgggattttg
      241 atgcgaatac ggatgcagca atggatatgc tgctcgacaa atggaatccc ccgatagggg
      301 atagagagct ctttctggag agatatgaag tgatcaaccg tcgcctatgg gatgaatatg
      361 aggcgagagt gataccaaaa gagtttctga ggcgtgaaag attccaccaa accctgagag
      421 aacattacgg aatagatgat gcagatgctg cggaacagat gggcagggat tatcttgatt
      481 tcatggctct tggtgacatt ctaatgcctg gggcccgtga ggtgcttgaa gctattgccg
      541 ctcgtggcgg caaaatggca attgtctcca acggcttcaa agaggtgcaa taccgcaagc
      601 tccgtatttc gaagattgac cactttttca gtgccgtgat gatttcggaa gaggtgggag
      661 tccataaacc ggattctctg atttttcaga aggcactcga ggcgctttgc ggagagggtg
      721 tggagattga tgaaaaagta aaggcccgca caataatgat cggagatgat tatcccaatg
      781 atatagtggg ggcaatcaac ttcggaatag atagtttcta ctacaatcct tccaataaag
      841 aaaatcccgg aaatgccgta tggcactccc gggatctact ttcactactg tctgattaga
      901 gcttgcgctt gatttcgaca atggtgtagg cctcaatgat atcaccgatt tttacatcgt
      961 tgaatccatc tatattcaga ccgcagtcaa atccggaagc aacctccttt acatcatcct
     1021 taaatcgctt gagcgatcca agtttgcctg tatagattac aataccgtct cggattaccc
     1081 ttacctttga tgtacgggtc agtttaccct ccttaaccat acagccggcg attgtaccaa
     1141 ctttggaaat cttgaaaatt tcgcgcacct ctgcagta
//
LOCUS       DAOSIE010000362         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_105224_length_1177_cov_2.933155, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000362 DAOSIE010000000
VERSION     DAOSIE010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105224_length_1177_cov_2.933155"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PLI69_07745"
     CDS             <1..1041
                     /locus_tag="PLI69_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOG42416.1"
                     /translation="SSITFPRFLMLPTKLFRQTLPRTDINLSYNFQNRPEYTRMIIST
                     AYGYNWNRNKRFYYQINPLQFNVVRIYNMDDDFYSKLNDPYLINSYRNHFDFGGGGML
                     YYTTNSATNPKDTYFYLRVQTDFAGNMLSIFNGGLKKDEKGSYMIWGVPYSQYVRTEV
                     SLVETLRFGRQNRMALAGRFLAGVGYAYGNSNVLPFEKFFYAGGSNSMRGWQSRSVGP
                     GNAPVSSSFAIANQTGDMRLEANLEFRFPLFWKLQGGLFIDAGNVWNLQRASIDGVAR
                     DERGIFTAENFGKSIAANWGLGLRLDFDLLLVRLDAGIKAYNPVTQQWHSPDMWFRKN
                     GYAIHFGIGYPF"
BASE COUNT          297 a          273 c          278 g          329 t
ORIGIN      
        1 tcctctatta catttccaag gtttctgatg ctccctacga agcttttcag gcaaacactg
       61 cctcgtacgg acataaatct ctcttacaac tttcaaaatc gtccggagta cacacgcatg
      121 atcatctcca cggcttacgg ttacaattgg aatcgcaaca agaggtttta ttatcagata
      181 aaccctctcc aatttaacgt agtgcgtatc tacaatatgg atgacgattt ctactcgaaa
      241 ctgaatgacc cctatctgat aaactcatac cgcaaccact ttgacttcgg cggaggcgga
      301 atgctctatt ataccaccaa cagcgctacc aatcccaagg atacctactt ttacctcaga
      361 gtacagaccg attttgcggg caatatgctt tcgattttca acggagggtt gaaaaaggat
      421 gaaaagggct cttatatgat atggggtgtg ccctattcac aatatgtgcg taccgaggtt
      481 tccttggtag agaccctgcg gttcggaagg cagaatagga tggcgctcgc aggtagattt
      541 ctggccggtg tcggttatgc ctatggaaat tcgaatgtgc tccctttcga gaaatttttc
      601 tatgcaggtg gctccaacag catgagggga tggcaatcgc gctccgtagg ccccggaaat
      661 gcgccggtaa gctcttcttt tgccatagct aaccagacgg gcgatatgcg tctcgaagcc
      721 aaccttgagt ttcgtttccc tctattctgg aagttgcagg gaggactctt cattgatgcc
      781 ggcaatgtct ggaacttgca gcgtgcctct attgacgggg ttgccagaga tgagcgcggt
      841 attttcactg cggaaaattt tggcaagagc atagcggcca actggggatt gggcctgcgt
      901 ttggacttcg atctgttgct ggtgcgtctg gatgccggta tcaaggccta taatccggtt
      961 acgcaacaat ggcattcccc ggatatgtgg ttccgtaaaa atggttacgc catccatttc
     1021 ggtatcgggt atcctttcta atttttaatt tttaattcga aacaaaaata aaaaaataca
     1081 gagatcatga ataacttcaa acttttatta gtcgcagctg tcgcattcat cgcatttgtt
     1141 gcggtttcct gcaacaccga aaggggagtg gcggact
//
LOCUS       DAOSIE010000363         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_105376_length_1176_cov_2.426405, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000363 DAOSIE010000000
VERSION     DAOSIE010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105376_length_1176_cov_2.426405"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..>1176
                     /locus_tag="PLI69_07750"
     CDS             10..>1176
                     /locus_tag="PLI69_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOG42417.1"
                     /translation="MIPDSERPIIPPINGTDIRTTLNIDIQNIADRALRNKLSESDDL
                     MAGTVIVMEVATGEIKAMVNLEKNDGGYFERYNYAIGRIGEPGSVFKITSLAILLENG
                     KVNLEDEMSALVNFNVGRTTFPDPYLRNYDKISIRRGFEISSNNVFRAQVWNHFGSSR
                     ADQEKYMNQLKAMKIDMKHDIEIDGIGKATIPHPSDKVRYWSDSDLASVAIGYAVEMT
                     PLHIITLYNAIANNGEMVRPHLISEYIKEGHSIKKVEPKVISTICSKSTAKELHKALR
                     GVVENGTGKNSFNGSKVEVAGKTGTARVVIPSNGAYVDSQGRKIHQGTFVGFFPYKNP
                     RYTVIAVVYSGLTNQNFYGGTWAAPIVREIAENIYASSPDWNGRMTASAQLPAYK"
BASE COUNT          374 a          246 c          270 g          286 t
ORIGIN      
        1 ggtaatcaga tgatacccga tagcgagagg cctattattc cgcctataaa tggtactgat
       61 attagaacca ctctcaatat agatatccaa aacatagcag atagagcact gcgtaataaa
      121 ctctcggaga gtgacgacct tatggcaggt acagttatag ttatggaggt cgctaccgga
      181 gaaatcaaag ctatggtcaa tcttgaaaag aacgatggag gttatttcga gagatacaat
      241 tacgctatag gtagaatcgg ggaaccgggt tccgtcttta aaatcacttc gctggcaatc
      301 cttctcgaga atggcaaggt caacttggag gatgaaatgt cagctctggt caattttaat
      361 gtaggcagga ctacattccc agacccctac ctgcgaaatt acgacaaaat atccatccgg
      421 cgaggatttg agatctcctc aaataatgtg ttcagagcac aagtatggaa tcatttcggc
      481 tcaagcaggg ctgatcagga aaaatatatg aatcagctca aggcaatgaa gatcgacatg
      541 aaacatgaca tcgagatcga tggtatcgga aaagctacca tccctcatcc ttcagataag
      601 gtcagatatt ggagcgatag cgacctggcc tcagttgcaa taggatatgc cgtagagatg
      661 actcctctcc atattattac tctttataat gccattgcca acaatggaga gatggtcagg
      721 cctcacctaa ttagcgagta tataaaggaa ggtcattcaa ttaaaaaagt tgagccgaag
      781 gtaatatcga ctatatgttc caaatctact gcaaaggaac ttcacaaagc tctaagaggc
      841 gttgtagaga atggaacagg caagaattcc tttaatggga gtaaagtgga agtagcggga
      901 aagacaggta cggcacgagt ggtaataccc tccaatggag catatgtcga cagtcagggc
      961 agaaaaattc atcagggtac attcgtgggg ttctttccct acaaaaatcc tcgttatacc
     1021 gtgattgctg tcgtctattc cggacttaca aaccaaaact tctacggcgg tacatgggca
     1081 gctcctattg taagagagat tgctgaaaac atctatgcat catcccctga ctggaacggc
     1141 cggatgaccg cctcagcaca acttcccgct tataag
//
LOCUS       DAOSIE010000364         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_106331_length_1169_cov_2.360862, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000364 DAOSIE010000000
VERSION     DAOSIE010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106331_length_1169_cov_2.360862"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1074)
                     /locus_tag="PLI69_07755"
     CDS             complement(43..1074)
                     /locus_tag="PLI69_07755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG42418.1"
                     /translation="MSDHFISVIVAVKNEEEHIGKCLLSLIHQDYPKDSYRIVVVDGM
                     STDQTKDIANEYRLRYGDFIHLYHNPKEWQAAGRNLAIRKEEQSDLVAYIDGHCIAEE
                     NWLKNLSEVLLHSDMNVAGVGSIIESPSDESGLGRAIDRIFSTTLGAAGTSYIPRKEL
                     TVVDTVPFVLYRKEALFKVGLYDEEMRYGEDYSLNFKLRRAGYRLLVNPGAKVYYYKR
                     KCVITFYMQMYNYGITKAILGKKYPTSLKSIHYLPSFSLIALLLFGLLGVIASEALYA
                     LGFIVTAYLSLVGLTSIYFSVKERYWDYIVSIPVAYFVEHIGYSIGFIVGLRKQGWLN
                     EDLSIESSV"
BASE COUNT          336 a          292 c          208 g          333 t
ORIGIN      
        1 ctaacttccc cgctgcctct tacactcctg cagaaccccc tcttatacgg aggattcaat
       61 agatagatct tcatttagcc acccttgctt cctaagtcca actatgaaac caatgctgta
      121 gcctatgtgt tcaacgaaat acgcaacagg aattgagaca atataatccc agtatctttc
      181 ctttaccgaa aaataaatcg aagtcagacc tacaaggctg agatacgcag taacgataaa
      241 tcctagagca tataatgcct cactagcgat aactccaagt aagccaaaca atagtagcgc
      301 tattaatgag aaactcggta gataatgaat tgacttaagt gaggtgggat atttcttccc
      361 cagaattgcc ttggtaatcc cataattata catttgcata tagaacgtga tcacacattt
      421 tcttttgtaa taatagacct tagcgcctgg atttaccagt aaacggtaac ctgctctccg
      481 taacttgaaa ttgagagaat aatcctcccc atatctcatc tcctcatcgt acaaaccgac
      541 tttaaataat gcctcctttc gatagagcac aaaaggaact gtatcgacaa ctgttagctc
      601 ttttcttgga atatacgacg ttcccgccgc accaagcgtc gtcgaaaata ttctatcaat
      661 cgctctcccc aaaccggact cgtctgacgg gctttcaata atcgatccga ctccggccac
      721 attcatgtca gaatgcagga gaacttcaga caaattcttc agccagtttt cctctgcaat
      781 gcagtgacca tcgatataag caaccaaatc actctgctct tcttttctga tggcaagatt
      841 cctgcctgct gcctgccact cctttgggtt atggtatagg tgaatgaagt caccatatct
      901 caatcgatac tcatttgcaa tgtctttcgt ttgatctgtg gacattccat cgacaacaac
      961 gatacgatag gaatcttttg ggtagtcctg gtgtattagg gacaatagac acttcccaat
     1021 atgctcttct tcgtttttta ccgccacaat cactgagatg aaatggtcgc tcattttgca
     1081 cccaatttag ccatttattt gatcatacaa cggctcgtga ctccattgga ctgagcatca
     1141 caggggtagt gctgccccga gtggagaat
//
LOCUS       DAOSIE010000365         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_106696_length_1166_cov_4.856886, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000365 DAOSIE010000000
VERSION     DAOSIE010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106696_length_1166_cov_4.856886"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..925
                     /locus_tag="PLI69_07760"
     CDS             206..925
                     /locus_tag="PLI69_07760"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOG42419.1"
                     /translation="MTKLSVNINKIATIRNARGGNLPDVIKAAIDCEKFGAQGITVHP
                     RPDERHIRYSDVRQLKPVLTTEFNIEGNPIPSFIDLVMEVRPAQVTLVPDAEDVLTSN
                     AGWDTKRNLSYLKKICKLFNEAGIRTSIFIDPVVEMIQYAAETGCNRVELYTEAYASG
                     YAAGREKAVEPYIKAAEEATRLGLGLNAGHDLSLENLSYLHRMIPNLLEVSIGHALIS
                     DALYLGLENTIAAYLEQLSDF"
BASE COUNT          342 a          259 c          240 g          325 t
ORIGIN      
        1 ttgggcacct caagacaatt ttccgtgagt ttctcaagta atggtgcata ccatacgaaa
       61 ctcacttttc tggatgaaag tgcatacaac aaaatagcaa attgctcgcg gtatacgctc
      121 cttgcctccc tgccaaaaat ggcaatcttt gtactctgtt tcatttatat cgctaaattt
      181 gtcaaaatta tgtatttttg taaatatgac aaaattaagt gtaaatatca acaagattgc
      241 aactatccgc aatgcacggg gcggaaatct tcccgatgta attaaagcgg caatcgattg
      301 cgaaaaattt ggtgcccagg gcatcacagt tcatcccagg cctgacgaaa gacacatcag
      361 gtatagcgat gtccgtcagc tcaaaccggt actgaccacc gaattcaata ttgaaggcaa
      421 ccctatcccc tctttcatag accttgtaat ggaggttaga cctgcgcagg ttacacttgt
      481 gccggatgcg gaagatgtcc tcacatccaa tgccgggtgg gatacaaaac gcaacctttc
      541 atatctgaaa aagatctgta aactcttcaa tgaggccggc atacgtacct ctatcttcat
      601 agatcccgtg gttgaaatga tacagtatgc tgctgaaacc ggatgcaacc gggttgaact
      661 ctatactgaa gcctatgcca gcggctacgc cgctggccgg gaaaaagcag tcgaacccta
      721 tattaaggcg gcagaggagg ccactcgtct gggtctgggt ctgaacgccg gtcacgacct
      781 tagtctggaa aatctcagct atttgcatag aatgattccc aatctgctcg aagtttcgat
      841 aggacatgca cttatcagtg atgccctcta tctcggactt gaaaatacaa ttgccgcata
      901 tttggagcaa ttgtccgatt tttaactata tttgcacgat atttgctttt gcaaagcata
      961 tcatttaaaa tataaatcac gtttttaaag attttttatg gaaaataatg ttcaatcatc
     1021 ctgcagcagt aacaggattc cgataatcct tagcgttctc gctctcgtta tagctatagt
     1081 tgtgctggta tttcaatttg taggtaaaaa aagttccaag ataaccatta ccgaagctaa
     1141 ttctgaagta gcagcccaga gaggtg
//
LOCUS       DAOSIE010000366         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_107304_length_1162_cov_1.769648, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000366 DAOSIE010000000
VERSION     DAOSIE010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107304_length_1162_cov_1.769648"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PLI69_07765"
     CDS             <1..1128
                     /locus_tag="PLI69_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOG42420.1"
                     /translation="DGSWYPIPKSQWYFELSRFTKGSQLYVISYDNRTLIPGVRLSAA
                     INVTRDKALDFYGFNGYESFYDSSLERGFYRMDRMVHTFKADFTGKIKGNFYWKAGYH
                     FSHFKTGAFTNENYTLPTTLFEIYKDFKIIPEEQADGGISSAIRVGLMYDSRDFEAAP
                     SRGIWAEANLAYAPKFLGTSVGYTKYNLTFRNYLPIAKDKLVFAYRLNLQGFIGKESP
                     AFYVLPYDMYLGTGYDREGFGGYRTVRGLMRGRIQGAHTCYFNTELRWRFVSFHLWKQ
                     NIAFALSGFFDGARVIKGFGNDFDIIRFSPFTPASSYYPPLGNLPYTLYGGKEKFHLS
                     AGGGIRFIMNRNFIVAFEYGKAFDIQDNSKGAFYINTGFLF"
BASE COUNT          327 a          254 c          257 g          324 t
ORIGIN      
        1 gacggatcgt ggtatcccat acctaaatca cagtggtatt ttgagctctc aagatttacc
       61 aaggggtctc aactctatgt catctcctac gacaaccgta ccttgattcc cggagtacgc
      121 ctttcagctg ccataaacgt aacaagagac aaagcgctcg acttttacgg tttcaatggt
      181 tatgagtcct tttatgacag ctctctggag agaggatttt accgtatgga tcgcatggtg
      241 catactttca aggctgactt taccggaaag atcaagggca atttctactg gaaagccgga
      301 tatcatttca gccatttcaa gacaggtgct ttcactaatg aaaactacac ccttccgacc
      361 acacttttcg agatctataa agatttcaag atcatacccg aagagcaggc tgacggcggt
      421 atttcaagtg ccatcagagt gggcttgatg tatgactcac gtgactttga ggcagcaccc
      481 tccagaggta tctgggcaga agccaatctc gcttacgccc ccaaattttt gggtacttca
      541 gtaggttaca ccaaatataa tctcactttc aggaactatc tgcccatagc aaaggacaaa
      601 ctggtttttg catacaggct gaatttacag ggctttatcg gtaaggaatc accggcattc
      661 tatgtgctcc cttacgatat gtaccttggc acaggatatg acagagaggg atttggtggt
      721 taccgaactg tgcgaggcct gatgagggga cgcatccagg gagcgcatac ctgttatttc
      781 aacactgaac tacgctggag gttcgtcagt ttccatctct ggaaacagaa tatagcattt
      841 gcattgagcg gattctttga cggagcaaga gttataaagg gatttggaaa tgactttgac
      901 attatcagat tctctccctt taccccggca tcatcatatt acccccctct aggcaatctc
      961 ccgtatacgc tttacggagg aaaagagaag tttcacttga gcgccggagg tggaattaga
     1021 ttcattatga atagaaattt catcgtggct ttcgagtatg gcaaggcatt tgatatacaa
     1081 gataatagca aaggtgcctt ctatataaat acagggttcc tcttttagtt tgatttaaaa
     1141 aaaagaatat gaaacgatta tc
//
LOCUS       DAOSIE010000367         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_109155_length_1149_cov_2.351920, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000367 DAOSIE010000000
VERSION     DAOSIE010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109155_length_1149_cov_2.351920"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="PLI69_07770"
     CDS             complement(<1..149)
                     /locus_tag="PLI69_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOG42421.1"
                     /translation="MRVSLAYSPCPNDTFMFHAMVEGLVDTEGLSFDVTLSDVEDLNM
                     SAPSLT"
     gene            complement(146..871)
                     /gene="mqnB"
                     /locus_tag="PLI69_07775"
     CDS             complement(146..871)
                     /gene="mqnB"
                     /locus_tag="PLI69_07775"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HOG42422.1"
                     /translation="MRILFTAATAEELHCAQTAYEKISPANAALIEVKFAVTGIGIAA
                     TCYNLTKILDSALLPAAKPYDLVVNIGIAGSYSEEFPIGSVAHITVEQFGDMGIETRG
                     GFQTLFDYAMLDANTLPFVDGLLKVSDPYPVTGPLEGVKDATGITFQTITGIREHNLR
                     RMESFSPHIESMEGAAFFYVCLMEKVPFIELRAVSNEVGERDKSRWDIRAAFSALENT
                     CVRLLEKAAEAGKDAKATGGAHR"
     gene            949..>1149
                     /locus_tag="PLI69_07780"
     CDS             949..>1149
                     /locus_tag="PLI69_07780"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HOG42423.1"
                     /translation="MAQDRTEEFDAEVTKQLASHYREILRLLGEDSTREGLLKTPERV
                     ARSMQFLTKGYEEDGSAIIQSAM"
BASE COUNT          298 a          259 c          287 g          305 t
ORIGIN      
        1 gttagggatg gagcgctcat attgagatcc tccacatcag atagggtgac atcaaaagag
       61 agtccctcgg tatctacgag cccctccacc attgcgtgaa acatgaaggt gtcgttggga
      121 cagggagaat atgcgagaga taccctcatc tatgcgcccc tcccgtagcc tttgcatcct
      181 tcccggcttc tgcggccttt tcgagtagcc ttacgcaagt gttttcgagt gcggagaagg
      241 ctgctctaat atcccaacgg ctcttatcgc gttcgcctac ctcattggaa acggcacgca
      301 actctataaa gggtactttt tccatcaggc aaacataaaa gaaggctgct ccctccatgc
      361 tctcgatatg gggtgaaaag ctctccatac ggcgcaggtt atgctctcga atgccggtta
      421 tggtttgaaa tgtgatgccg gttgcatcct taactccttc gagagggccg gtgacgggat
      481 aagggtcgct caccttgagc agtccgtcta caaatgggag agtattggcg tcgagcattg
      541 catagtcgaa aagggtctga aatcctccgc gagtctctat acccatatcg ccgaattgct
      601 ccactgttat atgagccacg ctgccgatcg ggaattcctc agaataactg cctgcaatac
      661 ctatgttgac caccagatcg tatggttttg ctgcgggaag tagcgcagag tccaggattt
      721 ttgtaaggtt gtaacaggtg gcggctattc ctatgccggt cactgcaaat ttcacctcga
      781 taagtgcggc atttgccgga gagattttct cgtatgctgt ttgtgcacaa tgcagttcct
      841 ctgccgttgc agctgtaaat aatattctca tagtgggcac taaaatagtt atttgttaga
      901 acttattcaa atattatttg taatattgcc taattataaa acatgaaaat ggcacaagac
      961 agaacagaag aatttgatgc ggaagttaca aaacaactcg cttcacatta tagggagatt
     1021 ttgagattac tgggagaaga tagtaccagg gagggtctgt taaagacacc cgaaagggtt
     1081 gcacgttcga tgcagtttct caccaagggt tatgaagagg atggttcggc aataatacag
     1141 tcggcgatg
//
LOCUS       DAOSIE010000368         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_112632_length_1125_cov_3.217757, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000368 DAOSIE010000000
VERSION     DAOSIE010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112632_length_1125_cov_3.217757"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLI69_07785"
     CDS             complement(<1..739)
                     /locus_tag="PLI69_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOG42424.1"
                     /translation="MNVTLEIDSYSGFCPGVVNAVRRAEDHLALHGTLHSLGPIVHNS
                     LEIDRLRDKGLRIVDLDSIDNLSGRVLLIRAHGEPPSTYETVTAKGITLIDCTCPVVL
                     RLQEKVRKAFLRMEKVGGTVVIFGKHGHAEVNGLVGQTEGRAVVIGNSDEISNVDFSR
                     PIELFSQTTKDPVEYDAIVKIMRARSKDVKVHNTICAQVSNRHKSLVNFARSHSMILF
                     ISGRESSNGKVLFDLCKSVNPRSWWIED"
     gene            complement(739..>1125)
                     /locus_tag="PLI69_07790"
     CDS             complement(739..>1125)
                     /locus_tag="PLI69_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOG42425.1"
                     /translation="VSPIATIPFVRNFVDSLLRGYGPQGVVMDGRDIGTAVFPEADLK
                     IFMVADAKIRAQRRVDQLVAKGEKADFDEIYRNVLERDYIDSHREMNPLRQAEDAVVL
                     DNSYVSVEQQMIWIGWVLKAKLDIDF"
BASE COUNT          305 a          305 c          223 g          292 t
ORIGIN      
        1 catcctcaat ccaccacgag cgagggttga cgcttttgca caggtcaaaa aggactttac
       61 cgttggaact ttccctgcca ctgataaaga gtatcataga gtggctgcgc gcaaaattca
      121 ccagtgactt gtgtctgtta ctcacctggg cacagatggt attgtgtact ttaacatcct
      181 ttgacctggc tctcataatc ttcacaatag catcatactc cacaggatcc tttgttgtct
      241 gagagaagag ctctattggc ctggaaaaat cgacattgga aatttcgtca ctgttaccta
      301 tgaccacggc acgaccttcg gtctgtccta caaggccatt gacctcagca tggccatgct
      361 tgccgaaaat cactactgtt ccgcccactt tttccattct gagaaaggcc tttcgcacct
      421 tctcctggag tcgtaacacc accggacaag tacaatcaat aagggtaata cctttggcag
      481 tcaccgtctc ataagtagaa ggaggttcac cgtgggctct tatcagaagt actctcccgg
      541 ataagttatc tatagagtca agatccacaa tacgcaatcc cttgtccctt aatctgtcaa
      601 tttcaagtga gttgtggact ataggaccca aagagtgaag agtaccgtgg agagccagat
      661 ggtcttcagc tctcctgacc gcattgacta cccccggaca aaagccggaa taggaatcta
      721 tttccagtgt aacattcatc aaaaatcaat atctaacttt gccttcaaca cccagcctat
      781 ccatatcatc tgctgctcga ctgatacata gctattgtcc agtaccaccg catcttccgc
      841 ctgacgcaga ggattcattt cacggtgtga atcaatataa tccctctcca gtacattacg
      901 gtatatctca tcgaagtcag ccttctcacc tttagccacc agctggtcaa cacgtcgttg
      961 agccctgatt ttagcatcgg caaccatgaa aatcttcagg tcagcctccg gaaaaacagc
     1021 agtgccgatg tccctaccat ccataacaac tccctgagga ccatagccac gaagtagtga
     1081 gtctacgaaa ttacggacga aaggtatggt cgctattgga ctcac
//
LOCUS       DAOSIE010000369         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_115969_length_1104_cov_3.346044, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000369 DAOSIE010000000
VERSION     DAOSIE010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115969_length_1104_cov_3.346044"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLI69_07795"
     CDS             complement(<1..395)
                     /locus_tag="PLI69_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOG42426.1"
                     /translation="MYNRDDRDQARFQQRDSEDVYSKPVRAGKRTYFFDVKSTKGGKD
                     FYLTITESKRRNNPDGSFTFDKHKIFLYKEDFDKFKDGLSEAIEYITQKCFGGEIPTR
                     PEISESDPESEEAAEETSDETKSFSVGKF"
     gene            528..>1104
                     /locus_tag="PLI69_07800"
     CDS             528..>1104
                     /locus_tag="PLI69_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42427.1"
                     /translation="MLNRRILRIKAFKVIYSRTENVTMTLKEGEAQLELSCEATRDLY
                     LFLLSIVEAVTREAENRINAAMGKFNPTEEELNPNLKFTRNRIASILSNDPDFLKIVK
                     KKKLSWEQYDVLLRHLYDTIKTRDYYKQYMVSPSDSLEEDAALWRAIFENEFEDNDEL
                     EAILEDLCIWWNDDLAYALNWCCRTMDELGKG"
BASE COUNT          298 a          216 c          252 g          338 t
ORIGIN      
        1 tcgaacttgc ctacggagaa agatttggtc tcgtcagaag tctcttcggc cgcctcttcg
       61 gattcagggt cagactctga aatttccgga cgggttggga tttctccgcc gaagcacttt
      121 tgggtaatgt actctatggc ctcgcttagt ccatccttga atttgtcgaa gtcttcctta
      181 tagaggaaaa tcttgtgctt gtcgaaggtg aaagagccat ccggattgtt gcggcgcttg
      241 ctctctgtaa tggtaaggta gaagtcttta ccgcctttgg tagactttac gtcaaagaaa
      301 tatgtacgct ttccggctct cacaggtttt gagtacacat cctcggagtc tctttgttga
      361 aatctggcct gatctcgatc atcacgattg tacataatat ttgtattatt tttgttaatt
      421 acagcatttt ccgtattcac gaatactgaa aatttgtcgt tatttaggca aatatatgtt
      481 ttttttgcga gtaaatgcta tctttgtcta aattttggaa aagaaaaatg cttaaccgaa
      541 gaattctccg aatcaaggca ttcaaagtaa tttattcgcg cacggaaaac gtcactatga
      601 cattgaagga gggggaagct cagttggaac tttcgtgcga ggccactcga gacctttatc
      661 tgtttcttct ctctattgta gaggctgtta cccgagaggc ggaaaatcgc ataaatgccg
      721 caatgggaaa gtttaatcca actgaagaag aactcaatcc caatcttaaa ttcactcgca
      781 accgcatagc atccattctt tcaaatgacc ctgacttcct taaaatcgta aaaaagaaaa
      841 aacttagttg ggagcaatat gatgtgcttt tgaggcatct ctatgataca atcaaaacgc
      901 gggactatta caagcaatat atggtgtcgc cctctgacag tcttgaggaa gatgctgcat
      961 tatggagagc gatttttgag aatgagtttg aggataatga tgagctcgag gccattttgg
     1021 aagatctctg catttggtgg aacgatgatt tggcttatgc ccttaactgg tgctgccgga
     1081 caatggacga gctgggaaag ggtt
//
LOCUS       DAOSIE010000370         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_116295_length_1102_cov_2.919771, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000370 DAOSIE010000000
VERSION     DAOSIE010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116295_length_1102_cov_2.919771"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLI69_07805"
     CDS             <1..724
                     /locus_tag="PLI69_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG42428.1"
                     /translation="MINEVNPDLLGIQEGIYEQVNYMAEHLPEYGRYGVGRDDGLNRG
                     EANAVFYKKSRFELLESTTFWLSETPDEVSMGWDAAFHRIVTMVRLLDKSSGRELYFY
                     NTHFDHIGKVAREESGKIIVQRMKNSIPENATVVLTGDFNAGHNDPILGPIEDYMTSA
                     RRAAPVTDSLNTFNGWGKIAPEGGGKIIDHIYFRNLVPLLFKTLTDAYSGVEYISDHY
                     PVIAIFSLKDARNQEARHESSK"
     gene            complement(678..>1102)
                     /locus_tag="PLI69_07810"
     CDS             complement(678..>1102)
                     /locus_tag="PLI69_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOG42429.1"
                     /translation="WHHRSDALSSVVVLIGILLSKYFWWMDSVLGIIIALMLFYATYT
                     IIKEAVTKILGEEPSDEIIAKITDAVAGMYPQGLQLHHFHLHNYVTHQEMTFHIKLPG
                     DLSLEEGHSVATEIEKMIQERFNITSTIHVEPLDYEHP"
BASE COUNT          298 a          224 c          264 g          316 t
ORIGIN      
        1 aatgataaac gaagtcaacc ccgacctttt aggaatccag gagggtatct acgagcaggt
       61 caattatatg gctgagcatt tgccggaata tggccgttat ggggtcggtc gtgatgatgg
      121 cctaaacagg ggtgaagcga atgccgtctt ttataaaaag agtcgtttcg aacttttgga
      181 aagcactact ttttggttga gtgaaacacc tgatgaggtt tcaatgggtt gggatgcggc
      241 ctttcatcgc attgtcacaa tggtgcgtct gctggacaaa agtagtggtc gagagttgta
      301 tttctacaat actcattttg atcatatcgg caaagttgcc cgtgaagagt ccggtaaaat
      361 cattgtgcag cgtatgaaaa actcaatacc ggaaaatgcg acagtggtat taaccggaga
      421 tttcaatgcg ggacacaatg atcccatatt gggccctatt gaggattata tgacttcagc
      481 gcgaagagct gccccagtta cagactctct caatacgttc aatggttggg gcaaaatcgc
      541 tcccgaaggt ggcggaaaaa taatcgacca tatttatttc agaaatctgg ttcctttact
      601 tttcaaaact cttactgatg cttattcggg cgtagaatat atctccgacc attatcccgt
      661 aattgcgata ttttctctta aggatgctcg taatcaagag gctcgacatg aatcgtcgaa
      721 gtgatgttga aacgctcctg aatcattttc tcaatttcgg ttgctaccga gtggccttcc
      781 tcaagtgaga gatctcctgg gagttttatg tgaaatgtca tctcctggtg agtgacgtag
      841 ttgtggagat ggaaatgatg tagttgtaat ccctggggat acatccctgc aactgcatcg
      901 gtaatcttgg ctataatctc atcagaaggc tcttctccga gtattttggt aacagcctcc
      961 ttgattatgg tataggtagc gtagaagagc atcagagcta taattatacc cagtacgctg
     1021 tccatccacc agaagtattt tgataacaga ataccgatca gtaccactac cgaggagagc
     1081 gcatcgcttc tgtgatgcca tc
//
LOCUS       DAOSIE010000371         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_117013_length_1098_cov_1.944391, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000371 DAOSIE010000000
VERSION     DAOSIE010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117013_length_1098_cov_1.944391"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLI69_07815"
     CDS             complement(<1..459)
                     /locus_tag="PLI69_07815"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOG42430.1"
                     /translation="MPNLRFPEFTEEWERCHLSDIADIIGGGTPNTSVSEYWGGNIQW
                     FTPSEIGKEKYVSLSERTISDIGLKNSSAKILPQGAVLLSTRATIGECSITLNECTTN
                     QGFQSLVAKINKVTSEFLYYLVSTIKKEMLRRSCGSTFLEISSSELKKIRV"
     gene            535..>1098
                     /locus_tag="PLI69_07820"
     CDS             535..>1098
                     /locus_tag="PLI69_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOG42431.1"
                     /translation="MRNKSFEEIVVLWQADKKQYVKRSTYSAYSLLINNHLLPAFTGV
                     DDVTETLVQSFVFTKLEQGLSQKSVKDILIVLKMILRYAVKQGYMLHREIDVKFPTER
                     EKQELEVLSRTNQKRIMEYVQSHFTFMNLGIYICLCAGLRIGEVCALVWDDIDVENGV
                     INVSKTIQRIYVIESGEKYTEVIIDTPK"
BASE COUNT          337 a          190 c          227 g          344 t
ORIGIN      
        1 gacacgaatc ttttttaact cgcttgaaga aatttctaaa aaagttgagc cgcacgacct
       61 ccttaacatc tctttcttaa tagtcgatac gagataataa aggaattcgg aagttacttt
      121 atttattttt gctactaatg attggaatcc ttgatttgta gtacactcat taagggttat
      181 ggaacattca ccaatagtag ccctcgtgct taatagcacc gctccttgtg gtagtatttt
      241 agcgcttgaa tttttaagac ctatatccga gatagtcctt tcgcttaaac ttacatattt
      301 ctcttttcct atttctgaag gagtaaacca ctgaatatta ccaccccaat attcagacac
      361 ggatgtattg ggcgtaccac ctcctattat atctgcaata tctgataaat gacacctttc
      421 ccactcctcg gtaaattcgg ggaatctcaa atttggcaca ttgaggttac aaggctatag
      481 cgagggaaat tgaggattct cgtaatatag tggtgttttt taaaccttaa tattatgaga
      541 aacaaatcgt ttgaagagat tgttgtgcta tggcaagccg acaagaaaca gtatgtcaaa
      601 aggtcgacat actccgccta ttcactattg attaacaatc atctcctacc tgcatttacc
      661 ggtgttgatg atgtaacgga aacccttgta caaagctttg tttttacaaa attggaacaa
      721 gggttaagtc aaaagtcagt caaggatatt ttgattgtgc tgaaaatgat tctccgttat
      781 gctgttaagc aaggatatat gttgcacaga gaaattgatg tgaagttccc gacagaaagg
      841 gagaagcagg agcttgaggt tttaagtcgg acaaatcaaa agcgtatcat ggagtatgtg
      901 cagagccatt ttacctttat gaatctggga atctatattt gcctttgtgc cggattgaga
      961 atcggagaag tttgcgcttt ggtttgggat gacatagatg tagagaatgg ggtaatcaat
     1021 gtatcgaaga ccatccagag gatttatgtt attgagagcg gagaaaaata tacagaagtg
     1081 ataattgata ctcctaag
//
LOCUS       DAOSIE010000372         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_117079_length_1097_cov_4.995202, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000372 DAOSIE010000000
VERSION     DAOSIE010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117079_length_1097_cov_4.995202"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLI69_07825"
     CDS             complement(<1..376)
                     /locus_tag="PLI69_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOG42432.1"
                     /translation="MDNYTIQETALLACLNDSQPLKKKDNIDYFRDLTLVHNIFTGVF
                     EHRRNIQEAYPEAVRPVIKRVEGARDKQLLDHYTDIIRRYQQKNIHIIRITDNEYPPK
                     LAKISDPPFVLYLKGRVESLSRP"
     gene            complement(369..998)
                     /locus_tag="PLI69_07830"
     CDS             complement(369..998)
                     /locus_tag="PLI69_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42433.1"
                     /translation="MCGNPIPDSFAETHPYCGVCKDSSDKHNPLVRVRAFGKYLFEDE
                     YPDDRLSKDIRRMKIDSTIIPLLQECLYYSMDRQYPELQELGVVVPVMSGSSDRGYNQ
                     AALLARGVASRYNLSYRDVLYAKEPYRPMHEIHDLAEKEREIAGKIGCNHQFEGDSIL
                     LIDDTCINMVTKRECTKVLKDHGAGEVWALVLGRMVTREHLNILRDRNG"
BASE COUNT          251 a          272 c          254 g          320 t
ORIGIN      
        1 caggtcttga taggctttct accctgcctt ttagataaag tacaaacggc ggatcagaga
       61 tttttgcgag tttgggggga tattcgttat ctgtaatccg tattatgtgg atgttttttt
      121 gttgataccg cctgatgatg tcggtatagt ggtcaaggag ttgtttatct cgtgcacctt
      181 caactctctt gatgaccggt ctgaccgcct cgggataggc ctcctgtata ttccggcggt
      241 gttcaaagac gccggtgaat atgttgtgaa caagtgtcag atcacggaaa taatcgatat
      301 tatccttttt cttcaggggt tggctatcgt tgaggcaggc gagcagtgca gtctcctgta
      361 tggtgtagtt atccattacg atccctcaag atattgagat gttctctggt aaccatccgc
      421 cccagaacaa gtgcccagac ttctccggct ccatgatctt tgagcacctt ggtacattca
      481 cgtttcgtta ccatattaat gcatgtgtcg tcgatgagga ggatagaatc cccttcaaat
      541 tggtgattac atcctatctt tcccgcaatt tccctctctt tctctgccag atcatgtatt
      601 tcgtgcattg gccgataagg ctcctttgca tagagtacat cccggtacga gagattatac
      661 cgggaagcaa cgcccctggc aaggagtgct gcctggttgt aacctctatc gctggaccca
      721 ctcattaccg ggactacgac accaagctcc tgcaactcgg ggtactggcg atccatggag
      781 tagtacaggc actcctgaag gagcgggatg atggttgaat ctatcttcat cctgcggata
      841 tccttactca gtctgtcatc agggtactcg tcttcaaaga ggtacttccc gaacgcccgg
      901 acacggacga gcggattgtg cttatccgaa ctatctttgc atactccaca gtaaggatga
      961 gtctccgcaa aactgtccgg tatcgggttg ccgcacacct cacaatacgg cggatagaga
     1021 atttcaagtt ttaccccatc agaccttaca aaaacctcca ccatattttt cactccttag
     1081 tatcctttgg aatcctg
//
LOCUS       DAOSIE010000373         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_118579_length_1088_cov_3.011617, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000373 DAOSIE010000000
VERSION     DAOSIE010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118579_length_1088_cov_3.011617"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLI69_07835"
     CDS             <1..704
                     /locus_tag="PLI69_07835"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOG42434.1"
                     /translation="GRVFSIREYEPDLIELSDVLLRAAKQVLVKLSPMVDISATLGEV
                     PCVKEVHVLSTDNECKELLLLMSGSGELLPSEAVPIHAVELSRDRESRFTFTIKEEKI
                     AQCPIATPGRYLHQPGRAILKAGAFKLPCVRFGISKIATSTHLYTSDTLIEGFPGKSF
                     RVEHIIPFNKEGIRTISREYPFINCVALNFPIDTNALKQRLKIRDGGNLHLFATTLAS
                     GEKVMIISDPLPVTF"
     gene            complement(694..>1088)
                     /locus_tag="PLI69_07840"
     CDS             complement(694..>1088)
                     /locus_tag="PLI69_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG42435.1"
                     /translation="YGIAGAGVYDWHLYDSHYTERYMDRPQDNPKGYEETNVLGRLSN
                     YKGDKTNMIRLTHGTGDDNVHFQNTLQLIDVLQKQNKDFELMIYPEAMHGYRGAQGRH
                     SAMQDYIFWYRYLLDSELPSDLLEYFKK"
BASE COUNT          282 a          259 c          237 g          310 t
ORIGIN      
        1 ccggcagagt cttctccatt agagagtacg aacccgatct gattgagctt agcgatgtgc
       61 tactcagggc tgcaaaacaa gtcctggtca aactctcgcc catggtcgac atttctgcta
      121 ctttgggaga agttccgtgt gtcaaagagg tacacgtcct ttctactgac aacgaatgta
      181 aggaactgct actgcttatg agtggctcag gagagctgtt gccttccgag gctgttccca
      241 ttcatgccgt tgagctaagc agagatagag agagtcgttt tacttttacc atcaaggagg
      301 aaaaaatagc ccaatgccca attgctactc cgggaagata ccttcatcag cccggtcggg
      361 caattcttaa ggcaggagca ttcaagcttc cctgcgtacg tttcgggatt tccaagattg
      421 ctacgagtac ccatctctat acatcggata ctcttataga gggatttccg ggaaaatctt
      481 tccgtgtcga acatattata cccttcaaca aagagggtat ccgcaccata tcgcgcgaat
      541 accctttcat aaattgcgtg gcactcaatt ttcctattga tactaacgca ttgaagcagc
      601 gtctcaagat tcgtgatggc ggtaatctac atctttttgc cactaccctc gcctccggcg
      661 agaaagtaat gattatctcg gatccactgc ctgttacttt ttgaaatatt ccagtaggtc
      721 ggaagggagt tccgaatcca gaagatagcg gtaccagaag atataatcct gcattgcaga
      781 gtgacgaccc tgtgcaccac ggtaaccatg cattgcctcg gggtaaatca taagttcaaa
      841 atccttattc tgtttctgta gtacgtcaat caactgaagt gtattctgga aatgaacgtt
      901 gtcgtcaccg gtaccatggg taaggcgtat catattagtt ttatcacctt tataattgga
      961 aaggcgtcct aacacattag tttcttcata accttttggg ttgtcctggg gcctatccat
     1021 atatctttcc gtgtagtgtg agtcatacag atgccaatca tacactccgg ctcctgcaat
     1081 tccatatt
//
LOCUS       DAOSIE010000374         1057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_123922_length_1057_cov_2.834331, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000374 DAOSIE010000000
VERSION     DAOSIE010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123922_length_1057_cov_2.834331"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1057)
                     /locus_tag="PLI69_07845"
     CDS             complement(<1..>1057)
                     /locus_tag="PLI69_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG42436.1"
                     /translation="SADSIQVLEGLEAVRKRPSMYIGDIAARGLHHLVYEVVDNSIDE
                     ALAGFCSEIAVVINPDNSITVSDNGRGIPTDTHEKEGRSALEVVLTVLHAGGKFSKDS
                     YKVSGGLHGVGVSCVNALSSHLTAEIHREGKIFKQEYSQGKPLYDVRVVGDTTKTGTI
                     ITFKPDDTIFTISTVYDYDTLARRLRELAFLNKGVRLTLMDDRAAEMVIDDHGNEVEA
                     PARKDVFYSQEGLLESVAYLDGSRDKLTENPIYLETDKIIPIEIAMQYNTDFKENIYA
                     YVNNINTIEGGTHLTGFRRGLTTTLKSYAEKNGFLAKLKFDLDPSDFREGLTAVISVK
                     VAEPQFEGQTKTKLGNS"
BASE COUNT          263 a          264 c          238 g          292 t
ORIGIN      
        1 tcgctgttac cgagtttggt cttggtctgg ccttcaaact gaggttcggc aaccttgacc
       61 gagatcaccg cggtaagtcc ctctctgaaa tcggaaggat cgaggtcaaa tttgagtttt
      121 gcgaggaaac cattcttttc agcatagctc ttgagagtgg tagtcaaacc gcggcggaag
      181 ccggtgaggt gcgtacctcc ctctattgta ttgatattgt tgacataggc atagatattc
      241 tccttgaaat cggtattata ctgcattgca atttcaattg gaataatctt gtctgtctcc
      301 agatatatgg gattctccgt aagtttatcg cgcgatccgt ccagataagc gacagactcc
      361 agtagtccct cctgagagta gaagacatcc ttcctggcgg gagcctcaac ttcgttgcca
      421 tgatcatcta taaccatttc tgcggccctg tcatccataa gggtaagtct cacacctttg
      481 ttgagaaatg cgagttccct caggcggcga gccaaagtat cataatcata tacggtggat
      541 atagtgaaaa tcgtatcatc gggcttgaag gtgatgatag ttcctgtttt tgtagtatct
      601 cctaccactc tcacatcgta tagcggcttg ccttgcgaat attcctgttt gaatatcttg
      661 ccttcacgat gaatctcggc agtcagatgt gaagaaagcg cattcacaca cgacacaccc
      721 acaccatgga gacctcctga aaccttgtaa ctgtctttgc taaattttcc tccggcatga
      781 agtacagtaa gtaccacctc gagagccgaa cggccttctt tttcatgagt gtccgtagga
      841 ataccacggc cgttgtccga aaccgttatg gaattgtcag ggtttatcac tacggctatc
      901 tcgctacaaa agcctgcgag ggcctcatcg atagagttgt caaccacttc ataaaccaga
      961 tggtgcaaac cacgtgcagc gatgtcaccg atatacatcg aagggcgctt acgcaccgct
     1021 tccagtcctt ccagcacctg aatactatct gcggaat
//
LOCUS       DAOSIE010000375         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_126303_length_1044_cov_4.327604, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000375 DAOSIE010000000
VERSION     DAOSIE010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126303_length_1044_cov_4.327604"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1044
                     /locus_tag="PLI69_07850"
     CDS             <1..>1044
                     /locus_tag="PLI69_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG42437.1"
                     /translation="AYYKWTQWAFLEMFNSYYCNGRRKARPISELIDHFSASGTEGVN
                     AACTEELNFTAEQWASFSEKEKADILMNYRIAYQGETAVNWCPMLGTVLANDEVKEGF
                     SIRGGFPVEQKKMRQWQLRVSAYAERLLDNLETLEWTDSLKEMQRNWIGRSQGAEMVF
                     KVSNGSQEYDMAIFTTRADTIFGVTFMVLAPESEWVEKLTTAEQRKAVDDYLVTAAKK
                     TERERMAETHKVTGVFTGSFAVNPLTEEKIPVWISEYVLAGYGTGAIMAVPAHDSRDY
                     LFAKHFNLPIVPLIEGCDISQESFDAKEGIMCNSGFLNGMSVQEAIPAAIDYVEAKGI
                     GQRKINYRLRDAI"
BASE COUNT          275 a          240 c          286 g          243 t
ORIGIN      
        1 ctgcctacta taaatggacc cagtgggcat ttctcgagat gttcaacagc tattattgta
       61 atggcaggcg caaggcacgt cccatatcgg agcttataga tcacttttca gcttccggaa
      121 cggaaggtgt aaatgcagct tgcacggaag aactcaattt tactgcggag cagtgggctt
      181 cattctccga aaaggaaaaa gccgatatac tgatgaacta ccgcatcgct tatcagggcg
      241 agactgcggt caattggtgc cctatgctgg gtaccgtact ggctaatgat gaggtcaagg
      301 agggattctc catccgtggc ggatttccgg tggagcagaa gaagatgaga cagtggcagc
      361 tgagagtctc tgcatacgct gaaaggctgc tggataattt ggaaaccctc gaatggaccg
      421 actcgctaaa ggagatgcaa cgcaactgga tcggccgttc gcaaggagcc gagatggtct
      481 tcaaggtctc aaacggctcc caggagtatg acatggccat ttttaccacc cgtgcagaca
      541 ccatcttcgg agttacgttt atggtgcttg cacctgaaag tgaatgggta gagaaactca
      601 ctaccgctga gcagcggaag gcagttgatg attatctggt gacggccgca aagaagaccg
      661 agcgcgaaag aatggcggag actcacaaag ttacaggagt attcaccgga tcttttgccg
      721 taaatcctct cactgaagag aagatccccg tatggatatc ggagtacgtg cttgcaggat
      781 atggtaccgg tgccataatg gcggtacctg cgcatgatag ccgtgactat ctctttgcaa
      841 agcacttcaa cctccccatt gtgccgctta tcgagggttg tgacatatcg caggagagct
      901 tcgatgcaaa agagggtatc atgtgcaatt ccggatttct caatggcatg agtgtccaag
      961 aggccatccc cgcagccatc gactatgttg aggctaaggg tatcggtcaa cgtaagatca
     1021 actatcgcct gagagatgct attt
//
LOCUS       DAOSIE010000376         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_130792_length_1021_cov_2.768116, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000376 DAOSIE010000000
VERSION     DAOSIE010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130792_length_1021_cov_2.768116"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1021
                     /locus_tag="PLI69_07855"
     CDS             <1..>1021
                     /locus_tag="PLI69_07855"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOG42438.1"
                     /translation="REVTCDTPKSVSDYIFSLGEGNTVLIVADMEWKQDGKVEKLLDL
                     KQEGFVRVASPEGIIFRIDEILQNMTDFEKIELYLLVDRIIVSDDEETRSRLLDSVQT
                     AFTRGNGRIAVFSDGLFKEFSNIFECDGIVFDRPAEHMFSYNNPLGACSGCGGFGKVM
                     GIDEALVIPDPSLSVYEGAVACWKGETMSRFLNDFIMESTQTGFPIHKPYNKLSQQEK
                     NILWDGVQGSTAITPFFKWIESKKYKIQFNYMLSRYSGKTTCRLCNGTRLKEQARWVK
                     IGGKDITELMQMQVGDLAKHFEALLPGSKSGIELDEYDYGIASRALKEIGQRLGYIKS
                     VGLSYL"
BASE COUNT          297 a          218 c          262 g          244 t
ORIGIN      
        1 gcagagaagt gacctgcgat acgcctaaaa gcgtctccga ttatattttt tcccttggtg
       61 agggtaacac agtgctaata gtagcggata tggaatggaa gcaggatggc aaggtagaga
      121 aacttctgga tctgaagcag gagggatttg tccgagtcgc ctcaccagag gggattattt
      181 tcaggataga tgagatcttg caaaatatga cggattttga aaagatagaa ctctatctgc
      241 tagtggaccg tatcatcgta agtgacgacg aagagacacg cagtcgactc ctggactccg
      301 ttcagactgc atttacccgg ggaaacggac gcatagccgt tttcagcgat ggtctattta
      361 aagagttttc aaacattttc gagtgcgatg gaatagtgtt cgaccgtccg gccgaacata
      421 tgttcagtta caacaatcca cttggcgcct gttcggggtg tggcggtttc ggcaaggtaa
      481 tgggtattga tgaagctctt gtaatcccgg atccctctct aagtgtctat gaaggtgcag
      541 tggcatgctg gaagggagag actatgagcc gttttctgaa tgatttcata atggaatcca
      601 cacagaccgg atttccgatc cacaaaccat ataacaagct ttcccaacaa gaaaaaaaca
      661 tactgtggga tggagtacag ggcagtacgg ccatcactcc tttcttcaag tggattgaga
      721 gtaaaaagta caagatacaa ttcaattata tgctctcccg ctactccgga aaaaccacct
      781 gccgtctctg caacggtacc agacttaagg aacaggctcg ctgggtaaaa ataggcggca
      841 aagatattac cgaactgatg cagatgcagg taggagatct ggcaaaacac tttgaagcac
      901 tgctgcccgg atccaaatcc ggaatcgaac tggatgaata tgactacggt atagcatcca
      961 gggcgctcaa ggagatagga cagcgactgg gctatataaa atccgtggga ctctcctacc
     1021 t
//
LOCUS       DAOSIE010000377         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_132364_length_1013_cov_2.855950, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000377 DAOSIE010000000
VERSION     DAOSIE010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132364_length_1013_cov_2.855950"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /gene="radC"
                     /locus_tag="PLI69_07860"
     CDS             <1..569
                     /gene="radC"
                     /locus_tag="PLI69_07860"
                     /inference="COORDINATES: protein motif:HMM:NF000642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOG42439.1"
                     /translation="GHANLNAIDLSREILLKAGNSLNILSTFTAQQLMRINGVGQAKA
                     LSILAAFELGRRMAVEIPEPMPQIHSPLQVNGIMSPIISHLPHEECWVLYLNRSNRLI
                     GKERVSQGGISSTIFDIRIIVKKAVEHLATGIILVHNHPSGNPRPSGEDMRQTEALRD
                     AAALFDISLTDHIIISKNKFYSFSDENL"
     gene            606..>1013
                     /locus_tag="PLI69_07865"
     CDS             606..>1013
                     /locus_tag="PLI69_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOG42440.1"
                     /translation="MKIINLGEKNSLLNRYIAEIRDVVIQKDSMRFRRNLERIGEIFA
                     YEISKTLTYFPKEVETPLGIANCNLSNDKIVLSTILRAGLPLHQGLLNYFDKAENAFV
                     SAYRKYKDALKFDIFIEYIASPDISGKTLIITDP"
BASE COUNT          300 a          264 c          203 g          246 t
ORIGIN      
        1 ccggccacgc aaacctcaac gcaatcgatc tctcgagaga gattctcctg aaggcaggaa
       61 acagcctaaa catcctttca actttcacgg cgcagcaact gatgaggatc aacggagtag
      121 gtcaggccaa ggctctctcc atccttgcag cattcgaact cggaagaaga atggccgtgg
      181 aaattcccga accgatgccg cagattcata gcccacttca ggtaaacggc attatgtcgc
      241 caatcatcag ccatctcccc cacgaagagt gttgggtact ctacctcaac cgttccaacc
      301 gtctcatagg caaggaacga gtcagtcagg gcggtatcag ttccaccata tttgacatca
      361 gaatcatagt caaaaaggcc gtggaacacc ttgctaccgg cataatactg gtacataacc
      421 acccttccgg caaccccaga ccgagcggtg aagatatgcg tcagaccgag gccctgcgcg
      481 atgctgctgc attgttcgat atttctctta ccgaccacat tatcatttca aaaaacaaat
      541 tttatagttt ttccgacgaa aatttgtaac ttagtagtcg agtataaaca aaacgaccac
      601 tgcatatgaa aatcattaac ctaggcgaaa agaactctct cctaaaccgc tacattgcgg
      661 aaattaggga cgtagttatt cagaaagaca gtatgcgttt ccgcagaaat ctggaaagga
      721 tcggcgaaat ctttgcttat gagatcagca agaccctcac ttattttccc aaagaggtgg
      781 aaactcctct gggcattgcc aattgcaatc tctctaatga caagattgta ctcagcacca
      841 ttctcagggc cggtcttccg cttcaccagg gccttctcaa ctattttgac aaagctgaaa
      901 acgcatttgt ctctgcctac cgtaaataca aagatgcttt gaagttcgat atatttattg
      961 aatacatagc ttcgcctgac attagtggta aaacactgat aattactgac ccc
//
LOCUS       DAOSIE010000378         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1709
            NODE_133605_length_1007_cov_3.596639, whole genome shotgun
            sequence.
ACCESSION   DAOSIE010000378 DAOSIE010000000
VERSION     DAOSIE010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863238
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:23:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,575
            CDSs (total)                      :: 1,551
            Genes (coding)                    :: 1,538
            CDSs (with protein)               :: 1,538
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 5 of 13
            Pseudo Genes (incomplete)         :: 7 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133605_length_1007_cov_3.596639"
                     /isolate="AMR_MDS_1709"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLI69_07870"
     CDS             <1..208
                     /locus_tag="PLI69_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG42441.1"
                     /translation="VTVGRSTGWSRCGHGNERSDGHMKCYIMEWELTLQDLALPHLIF
                     SSSVPQTVVEVLVPSSPKVTPEAP"
     gene            complement(315..>1007)
                     /locus_tag="PLI69_07875"
     CDS             complement(315..>1007)
                     /locus_tag="PLI69_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG42442.1"
                     /translation="ILKEVTRKRFACEPDAQQALEEARKRLRRHPLWKVDLSLETLVT
                     EKNPRGRPGKTPRPTIQITEWVIRASDPQLQQKVYLAELRKAESFVLMTNVTEEDTPA
                     REILRLYKGQKMVEDNFSAIKRPTMVDTLYLKKPERIAALVTLLAVTLLIQAVIRVLV
                     RRNLDALPEPPGLDHGCKPLIRPGLKKILRFIGYHSIITAGGERWPWCISLNHEKNLE
                     IWLRLLEFNVDR"
BASE COUNT          208 a          277 c          263 g          259 t
ORIGIN      
        1 ggtgaccgtg ggtcgctcga cagggtggag tcgttgtggt cacgggaatg agcgatctga
       61 tgggcatatg aagtgctaca ttatggaatg ggagctcact ttgcaagacc ttgctttacc
      121 tcacctgata ttttcctctt ccgtccccca aaccgtagtt gaggtgctgg tcccctcctc
      181 tcctaaggta actcctgaag caccgtagct cagcatcctc ccccgtagtc tcctaaaaaa
      241 cgctttacaa cgttgctttc atgagcgctt ccgctccctg tctttcctgc aagaggtcgt
      301 caagagcgaa ggtgttacct atctacattg aattccagga ggcgcagcca gatttccaga
      361 ttcttctcat ggttcagtga gatgcaccag ggccagcgtt caccaccggc ggtgatgatt
      421 gaatggtagc cgatgaaccg gaggatcttc ttcaagcctg gtcgaatgag tggtttgcac
      481 ccatgatcga gtcccggagg ctctggcaga gcatccaggt ttcgacgtac caatacccgg
      541 atcactgcct ggatcaggag agttaccgcc agcagcgtca cgagcgccgc aatacgctca
      601 ggtttcttca agtaaagagt atcgaccatt gtcggtcgtt tgatggccga aaagttatct
      661 tcgaccatct tctgcccctt atagagacgg agaatctcac gtgctggtgt atcttcctca
      721 gtaacgtttg tcatcaggac aaacgactct gccttgcgta gttcagccag atagaccttc
      781 tgctgcaact gcgggtcgct ggcccggatc acccactcgg tgatctggat cgttggccgg
      841 ggagtcttcc ccggccgtcc ccgtggattc ttctcggtga cgagagtttc aagagacaga
      901 tcaactttcc agagcggatg cctgcggagt ctcttacgtg cttcttcgag ggcttgttgc
      961 gcatcaggtt cacaggcaaa ccgtttcctg gtcacctcct tgagaat
//
